LOCUS       DAOSUD010000001       130124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_88_length_130124_cov_26.615235, whole genome shotgun sequence.
ACCESSION   DAOSUD010000001 DAOSUD010000000
VERSION     DAOSUD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 130124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 130124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130124
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_130124_cov_26.615235"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1100
                     /locus_tag="PLG49_00005"
     CDS             216..1100
                     /locus_tag="PLG49_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ15725.1"
                     /translation="MDINFELYKIFYYVAENLSFSKAAAKLYISQSAVSQAIKTLEDN
                     LNIKLFFRNTKQVKLTNDGEILFRHIEQAYNLIKNGERMIDEINSLERGEIRIGASDT
                     ICKYYLLNYFKEFHKLYPKVKILVTNGTSPKCVELLKQGSVDFIISNLPNQYADSSMK
                     IKKIKAIQDVFVAGNQFKELKNREIYLKDLENYPFLMLEKQTATRDFFDNMLVEYGTH
                     IIPEIELGSIDLLVEMAKIGLGISFVWEKCIEEQLKSDEIFIVNVKEKIPERYLGIIT
                     HKHIPLSIGAKKFIELLH"
     gene            1204..2280
                     /locus_tag="PLG49_00010"
     CDS             1204..2280
                     /locus_tag="PLG49_00010"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOQ15726.1"
                     /translation="MKLPSLKIGHLVADIPIIQGGMGIGVSRANLASAVANEGGIGII
                     SGVQIGFEEPDFETNNHEANIRGLKKEIKKARELSPKGIIGINILTAVNHYKELVLTA
                     VKEKIDLIICGAGLPVDLPELVKNSASKIIPIVSSAKAASVIIKIWDKKYQYLPDALI
                     VEGPKAGGHLGFPLEILNTHPLPSLSNILKEVLSVIKPFEEKYNKKIPVIAAGGIFNG
                     KDIAKYLKEGASGVQMATRFVATHECDAHINFKKAYLDAVKESIQIIKSPVGMPGRAL
                     RNEFTQSLEKNKEKIHKCYNCLKPCNPATTPYCISKALIHAVRGDTKKGLIFAGTTAY
                     KLNNIISVKELIRELVQEAELTLT"
     gene            complement(2335..2493)
                     /locus_tag="PLG49_00015"
     CDS             complement(2335..2493)
                     /locus_tag="PLG49_00015"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HOQ15727.1"
                     /translation="MLKTRIEQTREHLNDLIILGEENISSKDVMLVSQVLDDLLVEYL
                     KELDKDEL"
     gene            complement(2703..3959)
                     /gene="purD"
                     /locus_tag="PLG49_00020"
     CDS             complement(2703..3959)
                     /gene="purD"
                     /locus_tag="PLG49_00020"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOQ15728.1"
                     /translation="MKVLVVGSGGREHAIVWKLSQSKKVDKIFCAPGNAGIAQLAECV
                     NIKATDIEALADFAQDKAIDFTVVGMDDPLMLGIVDVFQERGLKIFGPTKNAAIIEGS
                     KSFSKNLMKKYNIPTANYEVFDDSKDALEYLKSQSFPIVVKADGLALGKGVLICNNYE
                     EAKEAVIDIMENKKFGDAGNKVVIEEFLVGQEVSVLSFCDGDTIVPMVSAQDHKRALD
                     NDQGLNTGGMGTFSPSRIYTQEIQNYCMEKIFKPTLEAMKKEGRPFTGILFFGLMVTE
                     NGVKVLEYNARFGDPEAQVVLPRLKGDLLEILEACVDKRLHEIKVEWEDNAAVCVVLA
                     SGGYPLSYDTGFPIFGLDNLHENNIIVFHAGTAKKDDQIVTNGGRVLGVTGIAKTLDE
                     AIRTAYQGVEKIRFENMHYRKDIGIK"
     gene            complement(3978..4607)
                     /gene="purN"
                     /locus_tag="PLG49_00025"
     CDS             complement(3978..4607)
                     /gene="purN"
                     /locus_tag="PLG49_00025"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ15729.1"
                     /translation="MYKIGVLVSGGGTNLQAIIDAIENGKIKNGKIVTVVSNKPDAYA
                     LERAKKHQIEGICISPKDFDSKEVFTDCLIKHFEERQVDLVVLAGYLLVLDEKFVKRF
                     ENRIMNIHPSLIPSFCGAGYYGLKVHQAVLDRGVKITGATVHFVDEGTDTGPIILQKA
                     VQVKEEDTPESLQRRVMEEAEWEIYPKAIQLFCDNKIMIKDKKVKILED"
     gene            complement(4595..5620)
                     /gene="purM"
                     /locus_tag="PLG49_00030"
     CDS             complement(4595..5620)
                     /gene="purM"
                     /locus_tag="PLG49_00030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOQ15730.1"
                     /translation="MDYKSAGVDVEAGYEAVLLMREHVKATFRPEVLTDIGGFGGLFS
                     IANIKMKEPVLVSGTDGVGTKLKIAFLADKHDTIGIDCVAMCANDVVCSGAEPLFFLD
                     YIACGKNIPKKIAEIVKGVAEGCKQAGAALIGGETAEMPGFYPEDEYDLAGFCVGIVD
                     KEKIIDGSKIKKGDLLIGLASSGIHSNGYSLIRKIFNPNKNNINEYVELLGCTLGEEL
                     LKPTKIYVKTIQKLKEEVEIKGISHITGGGFIENIPRIFPEGLGGKIYEGSWDIPPIF
                     KLAEELGNVSRMSMYNTFNMGIGMVVIISETEKEKALSLLSGMGEMAYVIGEVVESET
                     GVEICTK"
     gene            complement(5610..7022)
                     /gene="purF"
                     /locus_tag="PLG49_00035"
     CDS             complement(5610..7022)
                     /gene="purF"
                     /locus_tag="PLG49_00035"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOQ15731.1"
                     /translation="MQRELLDDKFHDECGVFGIYNKNNWNTAEMTYFGLYALQHRGQE
                     SAGIAINDNGTILYHKEMGMVSEVFDEVIINHLHGQMAIGHVRSAMSGENQKENTQPL
                     VLKYTKGNMALAHNGSLVNAAKIRNDLEEKGFLFQSYSDAEVIAALLSKERIRHHSIE
                     ESLVEVMKQIKGAYSLLVMTPHKLIAARDPLGMRPLCIGKKDDSYVFSSETAALETIG
                     ANFVRDVKPGEIVVVNEEGITSIQTEAPKESKMCIFEFVYFARPDSIIDGADVYEARL
                     EAGKILAREHPVDADLVIGVPDSGLTAALGYAMESGIPYGDGLMKNRYIGRTFIRPSQ
                     EMREQAVRLKLNPLKSQVDGKRIIMIDDSIVRGTTSKRIVKMLKEVGAKEVHIRVSSP
                     PVYYPCYFGIDTPHKENLIANHYSIEEIAELIGADSLAFLSIEGLKKTPIGAKCGFCT
                     ACFDGNYPINVDKEEKEYGL"
     regulatory      complement(7202..7303)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(7380..7898)
                     /gene="purE"
                     /locus_tag="PLG49_00040"
     CDS             complement(7380..7898)
                     /gene="purE"
                     /locus_tag="PLG49_00040"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOQ15732.1"
                     /translation="MVEVGIIMGSDSDLPVMIEAARVLDEFGIEYELTIVSAHRTPQR
                     LYEYAKNAKEKGLKVIIAGAGGAAHLPGMVASITSVPVIGVPVKTSTLSGIDSLYSIV
                     QMPPGIPVATVAINGAKNAGILAAQIIGSFNLEVGKKVEAYKESLKDMVEEKAQELEK
                     IGGESYLLKMKK"
     gene            complement(7913..9082)
                     /locus_tag="PLG49_00045"
     CDS             complement(7913..9082)
                     /locus_tag="PLG49_00045"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOQ15733.1"
                     /translation="MTYGILNQTIGIIGGGQLGKMMLLEAKKMGFYAVILDPTFHCPA
                     HSLADEHIVASFDDEVAIRKLADRVDVITYEFEHIGVEVLKELEEEGHIIYPTPKSLA
                     IIQNKYKQKELLKKKRIPVPDFIYVSSVEDMKEAGKLFGYPFILKSCRGGYDGKGNMV
                     VNSPEELLFAFESLGGETEELMAEEFVPFTKEISVLACRGIDGETAVYPVGENIHQNN
                     ILIKTMVPAQISKTLTSRAMNLANRVMKVFEGVGMFCVEMFVTEDKKLLVNEVAPRPH
                     NSGHYSIEGCVTSQFEQHIRAITGIPLGDTSLIQPTVMRNILGEELGAGEPEICGVKE
                     ALSIPGVFLHIYGKEKTSPRRKMGHITAVAKTLEEAIQKVDTAAEFIKIYPVNYK"
     gene            complement(9130..9780)
                     /locus_tag="PLG49_00050"
     CDS             complement(9130..9780)
                     /locus_tag="PLG49_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15734.1"
                     /translation="MGNFREMNLKEKIEHVIYYYKWHFIFGVVILVFAINIILSIFQA
                     DKNIPLMTFSLQNGDLIDLEKKANWEKIFTEKIIKEEDLGRSVRVDFYIINTEEPNEA
                     TNISIQKLATYSAVGELDIVCFEENYYKEQMKIGAFLPLDTIPELSSVLENNRDKLVS
                     TVLENGEEENHVYGIKADDIKAFEGLEYDTKGKIVGISAGTKRLNYSIEAFKHLFE"
     gene            complement(10031..10777)
                     /locus_tag="PLG49_00055"
     CDS             complement(10031..10777)
                     /locus_tag="PLG49_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HOQ15735.1"
                     /translation="MMRDFFGENYKGKAFELFSAEHITVIVIIFACCLMIYLFHHRLK
                     EEKINRRFRHGLAFLLITIEVLYYIWVYVNHLWSFSRELPLHLCDMALIGTIILLFTK
                     SYFLYEFLYFWAMCGVLAALITPELSGYNATHFLFYHFFIGHGLIVIAIIFMTFVNEY
                     RPKVSSIFKTMLISNLYMLFVAAVNLITGGNYLFICRKPQVVSIMDFLGPWPWYILSM
                     ELVALVAFLIAYLPFIIYIRLKKSHARKGM"
     gene            complement(10901..11482)
                     /gene="pyrE"
                     /locus_tag="PLG49_00060"
     CDS             complement(10901..11482)
                     /gene="pyrE"
                     /locus_tag="PLG49_00060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOQ15736.1"
                     /translation="MLNKERVIEIFKETEVLLEGHFLLTSGRHSNQYMQCAKILQYPN
                     YAEEIAKGLAEEFKDDQIDIVVGPAMGGIIIAYELARQLNCKNLFAEREDGKMTLRRG
                     FTIPEGARVLVAEDVITTGGSVKEVIDIVKEQGGELAGVAVLVDRSNGNIDFGTKLRA
                     ALTVDVVSYEAEECPLCKEGKLPLVKPGSRKIK"
     gene            complement(11526..12440)
                     /locus_tag="PLG49_00065"
     CDS             complement(11526..12440)
                     /locus_tag="PLG49_00065"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOQ15737.1"
                     /translation="MIKEKHNLKVNIAGVELKNPVTTASGTFGSGREYGEFVDLNQLG
                     AVTVKGVSSTPWKGNPAPRIAETYGGMLNSVGLQNPGVEEFIKNDIPFLRQYDTKIIV
                     NVAGKSVSEYCEVVERLSHADIDLIELNISCPNVKEGGVTFGTDVKMAHKVTKEVKRY
                     AKQPLIVKLSPNVTDITEIARAVVDGGADGISLINTLLGMAIDIHKRKPILANTVGGF
                     SGPAVKPVALRMVYQVARAVSVPIIGMGGISTGEDAIEFILAGATAVAVGTANFRNPR
                     ATIDVLEGIKEYMVRYGIQSLDEIRGMI"
     gene            complement(12433..13206)
                     /locus_tag="PLG49_00070"
     CDS             complement(12433..13206)
                     /locus_tag="PLG49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOQ15738.1"
                     /translation="MKEIKHALILDNQLIADNIYKMTLEADGIAREVKAGQFVNLYCK
                     GEAKLLPRPISICEIDKDRGRITLVYGVVGQGTKEFSTLKAGEKIEVLGPLGNGFKID
                     ESKAKNIIVGGGIGTPPLVELVKQLKGEKEVYLGFRSQSILLEAFKVYGANVHVATED
                     GSEGFKGNVLELLKKNNAKGEIIYSCGPKPMLKAIAAWAKENEIAMQVSLEERMACGI
                     GACVGCVCKIKEKDELDWQHKKVCKDGPVFWSDEVVWDD"
     gene            complement(13193..16414)
                     /gene="carB"
                     /locus_tag="PLG49_00075"
     CDS             complement(13193..16414)
                     /gene="carB"
                     /locus_tag="PLG49_00075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOQ15739.1"
                     /translation="MPRDLSIKKVMVIGSGPIVIGQAAEFDYSGTQACQALREEGVEV
                     VLINSNPATIMTDKEMADSIYIEPITIEAIERIIEKERPDSLLAGMGGQTGLNMAVEL
                     YDKGILDKYGVRIIGTSIESIKEGEDREGFKKLMEKIGQPVVESAIVTNVEDGIKFAE
                     KIGYPLIVRPAYTLGGTGGGIANNVEELKEILAHGLHLSRVGQVLLERSIKGWKEIEY
                     EVIRDEDGNRIVVCNMENIDPVGVHTGDSIVVAPCQTLSEKENKMLSNAALDIIDAVG
                     IKGGCNVQFALNPESFEYAVIEINPRVSRSSALASKATGYPIAKVATKIALGYRLDEI
                     KNSVTGKTYACSEPTIDYVVIKIPKWPFDKFHGAKRSLGTKMMATGEVMAIGNNFEAA
                     LLKGIRSLEIGQYSLNKKSSSGRSLDELKKRVQVPDDERIFDLAEMLRRSYRVEKVCK
                     ITGMDPFFVEKIKWIVEQEENLKQMQPEDLTKEYLYLLKKKGFSDKGIADLMGVSPQL
                     IYQLREKWDIRPVYKMVDTCAGEFDAESPYYYSTYDEYDEVEVSNKKKVMVIGSGPIR
                     IGQGIEFDYCSVHSILSLKKAGIETIIVNNNPETVSTDFNISDKLYFEPLTEEDVLNI
                     VEKEKPDGVILQFGGQTAIKLAKFFREMNIPILGTKPEQIDMAEDREKFDALLEELKI
                     KRPKGKAVWTLEEGLEEAGELGYPVLVRPSYVLGGQGMEITYSEEELKQYLGEAFERD
                     SKNPVLIDRYLLGREIEVDAICDGKDILIPGIMEHLERAGIHSGDSITIYPSQNISKE
                     IKEKILDYTKRISLALNVIGMVNIQFIEYENELYIIEVNPRSSRTVPYISKVTGVPVI
                     DIATRVMLGESLKDIGYGTDLYKEAELIAVKVPVFSTEKLPMVEVSLGPEMRSTGEVL
                     GVGKTLEEALYKGFLAAGIKIPKGNGTILATIKPYDQEEFLPIAKAFADYGYRFIATE
                     GTAKLLEKANIPVSEVKKISEGVPNILDIIRSGMIDMVINTPTKGNDSRRDGFRIRRA
                     AIEASVDLFTSLDTVKALLKVMDSKISFNDIEVYNLGGSHERN"
     gene            complement(16399..17454)
                     /locus_tag="PLG49_00080"
     CDS             complement(16399..17454)
                     /locus_tag="PLG49_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOQ15740.1"
                     /translation="MRAKLILEDGTTFNGNAFGYMKEAVGEVVFNTQMTGYQELLTDP
                     SYYGQIVAMTYPLIGNYGINLEDIESETVKVKGLIVREKADFPNNWRCEMELDGYLKQ
                     NKVIGIEGIDTRALTKILSKKGTMRGIITVRDLTASQIEQKIRSYHNINAVLEVTTKE
                     KYVIEAKGHHIAIIDFGVKQSIIECLKERNCAMTVFPAGTTPEEILESNPHGILLSNG
                     PGNPCDISWAVENIKKLIGKKPILALDLGCQLLALALGGTVKKHGHRGANHPVKDLGT
                     NKILITTQNHSYGIEELPGDTIKTHVNINDDTIEGFVHKDLPLFGVQFYPGNRDIGNI
                     YSCFIRAMEGESVCQGI"
     gene            complement(17451..18377)
                     /gene="pyrF"
                     /locus_tag="PLG49_00085"
     CDS             complement(17451..18377)
                     /gene="pyrF"
                     /locus_tag="PLG49_00085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOQ15741.1"
                     /translation="MIDRLIHQIEMKENPTVVGLDPRLDFVPAFIKEEAYESWGKTPK
                     AAAWAFLKYNKAIIDAVYDLVPAVKPQVAMYEQFGPEGIQAYIDTILYAKEKGLIVIG
                     DIKRSDIASTAEAYADGHIGKVKVEDEAFEVYHEDSITLNPYLGYDSIEPYIKHCKDY
                     DKGLFILVKTSNPNSGEIQDLDLGGEKLYERVGKLVSDWGKELIGKKGYSQVGAVVGA
                     THPEQAKRLRELMPHTFFLVPGYGAQGATAKDLAVCFDKNGLGAIVNSSRGIIAAYKL
                     DKYKKDYSEKEFALASRQAVIDMKKDLQGAFA"
     gene            complement(18393..19682)
                     /locus_tag="PLG49_00090"
     CDS             complement(18393..19682)
                     /locus_tag="PLG49_00090"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOQ15742.1"
                     /translation="MKLLIQNGRLLDPLSRTDGKKDILVEDGKIIALADKIEDSADRI
                     IDAEGLWVTPGLIDVHVHLREPGYEYKETIKTGSRAAAKGGFTTICCMPNTNPVIDNE
                     ILVEYIKMKAEREALVNVLPIGSITKGQKGEELSNIGKMVEAGICAISEDGKSVENAG
                     LLKKAMKYASMYNIPVLSHCEDKSLAGDGVMNAGEMAQRLGMKGISADSEEVIVARDI
                     ILGRSTGVKLHICHISTAGSVQLIKEAKMRGEKVTAEVCPHHFSLTDEAVEGYDANTK
                     MNPPLRSKKDVESIKQALKENIIEIIATDHAPHHIDEKNCEYDKAAFGIVGLETAVAL
                     GITHLVEEGVLSPLELIEKMSTNPAKLLGINKGSLETGKIADITLIDPNEEYVIDVNH
                     FESKSKNSPFHGYKVKGRVKYTIVAGKIVLDNGEIVD"
     gene            complement(19669..20604)
                     /locus_tag="PLG49_00095"
                     /pseudo
     CDS             complement(19669..20604)
                     /locus_tag="PLG49_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
     gene            complement(21210..21914)
                     /locus_tag="PLG49_00100"
     CDS             complement(21210..21914)
                     /locus_tag="PLG49_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15743.1"
                     /translation="MTKIYYYNEKLMKKIRKNKVSKKEKIKDFISIILFFVVAFGVTL
                     FIFNYKGFGFINSDKMIIQIIEFLLMYPIVCFIIVLLMALLEFLLAILKSIINKILGK
                     DSGKRYFSDTFRSNFARKNKIKLVLADDKMTVLYEYYEGAGDWTTDAYGIDTIYYSDI
                     KRIVYTPADFYIHIYCKYTFNKTLSDYNRIYKTYDHEGWYNFCLLFEDAEGFLKDLAL
                     KANFQIEKIYDEKYDL"
     gene            complement(21982..22683)
                     /locus_tag="PLG49_00105"
     CDS             complement(21982..22683)
                     /locus_tag="PLG49_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15744.1"
                     /translation="MFEEISMNKNLIRDSFLKERYNNIKKKLTILAVLFPLIITLIIG
                     MGLVLFFYIEGFDLIIDTDVIDTDVYIPLFFLLVFGTVFIRALIFGFIIKYIVQWVYR
                     KKLVKKDRRTYRYVNCNIDNRENIKTFFTDSSLLYQYDRYNSPGLENTESNSKIKVEI
                     KYSDVERLVRSTVDEVLHVYCNYEEEIDNFSSNKRGWYHLPLVYINNMYFIDNFAQKT
                     GRICEIIEDEKYSLN"
     gene            complement(22811..24508)
                     /locus_tag="PLG49_00110"
     CDS             complement(22811..24508)
                     /locus_tag="PLG49_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HOQ15745.1"
                     /translation="MIIGIDLGTTNSLVSYFTEDGPKIIPNRLGKKLTPSVVSIDEND
                     QVYVGETAKERMILYPDTCASVFKRDMGSKKKYTLGHRTFLPEELSSFVLRSLKEDAE
                     VYLGEKVTEAIISVPAYFNDARRKATKRAGELAGLKVERIISEPTAAAIAYGLYQSNK
                     DMKFLVFDLGGGTFDVSILELFGNILEVHAVAGDNFLGGEDFTQVLIDMFMKEHNLKA
                     EELDIKTLSHIRKQAEECKKKFAQESSSVMSCNINNELMEMKIDIDSYEKNCEILLER
                     IKQPIKRSLSDANIKLSDIDQVVMIGGATRLPIIHKMVSKLFKMIPNTSINPDEAVAL
                     GAAIQGAMKERNQAVKEVILTDVCPFTLGTEVVREIEENRFESGYYCPIIERNTVIPA
                     SRTERLYTVYDNQTKIRVKILQGESRFASNNVLIGELTVEVPKGRAGEEAVDVTYTYD
                     INSILEVEVKVVSTGKTERIIIKGQDVDMTDEEIEKRMQELSYLKIHPREHEANKLLL
                     LKAERLYEESIGEKRKRLEYELLKFDSALNSRDKVKIEEARKRLQTVLDFIIDESDF"
     gene            complement(24505..27909)
                     /locus_tag="PLG49_00115"
     CDS             complement(24505..27909)
                     /locus_tag="PLG49_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ15746.1"
                     /translation="MDKKKVFNILEIQETKDESIIKAAYRKKLLVTNPEDDPEGFKLL
                     REAYEEAIRLSKIEEEEKEDTPVSRWIEKVDSLYRSLSRRNNIDAWEELFQEEVCRNF
                     DTFEEVRDALLLYLMDHFRLSFDIWELINNTYHLPEIKEELYEKFPINFIDFVIRAPE
                     ADKWFDYKLFEGKEDGDVDEFINCYYALRRMNDTKEYGEFHKVLDTIEKTNLWHPYLD
                     VEKMIYYMNSNELETAKEIGQKLMERKYDNLFIKYYLMLLTMESGDIEKAYKEAEEII
                     KESPRHYGARKAVYKYFYKKGDYKQSKEKLLELLEEWSYDQSLIEDLQKVNEHLIEIY
                     KEQIEKEPENKKLKLELGWSLFQNNLFEECLNLIKDMELDDKIYYEYYNLMTRNYMVM
                     ENYEQAYPYAKIWLEEILKIKEKIKDKDDADISDENKKIIRRLSLAYYFIAKCYYHFS
                     KSKTDENYIDKCIEYYDLAIETETNEGDLLKFLCEKAQLYLELGKNELCVDVCDRIIS
                     KDSGYFPAYVYRQEAFYNLKMGREVLDDYYKAVEIYDGYPKIYILAVKVLIIFERYEE
                     AKRVLDKAKEAGIKSNELAYQELRIKRIKATTKEEKREIADEIKKLYDKVQKEKTEQE
                     EKNGQEIVNDIEDSSVLLYELSFCYYEMDEFDLALKAINERLKVSDDVSSIELKGDIL
                     YYLKKYKEAINVFKKLINDCPNYYYGYYVTGYCYEELNNDQKALEYYLKTVELEPEHS
                     YVNHKIKDIYRRWYNKYFKREHYELAVKHAKRQVEIQPNGYYYNELGLIYSDGYEMEK
                     AIEAFKKAVEYDKEDMYPYNNIGWSYKVLGNFDEAYKYYQLALKYCTTNELITHRNLS
                     TYYMVVGEFEKTIELYEEIYEKTENSDFLEYIAYVYVRLKNWEMALAYYKRKLDIDKN
                     YVNQYWLDVGYLYSCKGDNKNAIKFYKKGIFQDVIGSEPYRYMGDHIFWLLRDYKKAL
                     KYYKKAYMYFEYYNYDNVYFEELTECILYVLKILGKEKLAKKYFEKTLDYINRKFNSL
                     DSWLNNPNERKERLYYMASWHLSLGEYEKAKEYVEQMKKCLSCQRCSFLKCAKLYFIE
                     GLFLEAEQDYHSALEKYQIALKLDENDIKYIGKVNEMKEKIGEKQL"
     gene            complement(28149..28577)
                     /locus_tag="PLG49_00120"
     CDS             complement(28149..28577)
                     /locus_tag="PLG49_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15747.1"
                     /translation="MADKKKELQNKQKSSYKVYQEEHSKSSLGNFFLGLILLCSGIYL
                     IFQNTIISTGFSLSRILGFTPSFGFVLLPLLIGIIMLFFNTKSKLAWFFIAFGIIIIL
                     LGLLMGLRITFMPVTLYETILMFGLTAAGLGLVLRGLLGR"
     gene            complement(28684..29751)
                     /gene="prfA"
                     /locus_tag="PLG49_00125"
     CDS             complement(28684..29751)
                     /gene="prfA"
                     /locus_tag="PLG49_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOQ15748.1"
                     /translation="MFSKLEEINERYDEISKKISDPEVINNQTEWQSLMKEYGDLKPL
                     VEKYTEYKKVKEAIDEAHQLLEDNLEEDFRQLVKEELAENKQKIVKIEEELKILLLPK
                     DPNDEKNVIVEIRGGAGGDEAALFAGDLFRMYSMYAEKNNWKVEIMDSNEIGVGGFKE
                     IIFMIQGRGAYSKLKYESGVHRVQRIPITESGGRIHTSTVTVAVLPEVEEVDVEININ
                     DIRVDVFRSSGHGGQSVNTTDSAVRITHLPTNIVVTCQDEKSQLKNKEKALKVLRAKL
                     YEIEREKQEKELAETRRSQVGTGNRNERIRTYNFPQGRVTDHRIGLTLHKLDSILDGD
                     LDEIIDSLIAFDQMERLKKGS"
     gene            complement(29775..30635)
                     /gene="prmC"
                     /locus_tag="PLG49_00130"
     CDS             complement(29775..30635)
                     /gene="prmC"
                     /locus_tag="PLG49_00130"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOQ15749.1"
                     /translation="MKKTIDELLREAKGLLKDKGIKTWALDSELLLSHLFSFSRVQLY
                     THSKEEVEEAIQEKYRDLILERIKGMPVQYLTNTKEFMSLPFYVDQNVLIPRNDTEVL
                     VEKALELIDEKESMILDMCTGSGCIAISIAYYKKKSKVLGLDLSAPALKVAQYNARLN
                     GVDDRVYFIQSNLFESLQEDYLSKFDMIISNPPYIPTEDISRLEVEVKAYEPFMALDG
                     GKDGLDFYRKIIEKSKAYLKDKGILIFEIGYNQGKAVTEIFKSHGFSDISIKKDLAGL
                     DRVVFGKFIK"
     gene            complement(30619..31536)
                     /locus_tag="PLG49_00135"
     CDS             complement(30619..31536)
                     /locus_tag="PLG49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOQ15750.1"
                     /translation="MKRTSVGGQAVIEGVMMRSPKGYAVAVRKPDKEIVIEKKELTAL
                     TEKYPVLKLPLLRGIVTFIDSMVIGIRTLSYSAQFFDIEEAEPSKFEKYLDNKFGSKK
                     LEDFIIGFTIFFAFVLGIALFMVTPLLISRLFKNFTESKWLLNLFEGLIRIIIFLSYI
                     YLVSKMKDIRRVFEYHGAEHKTINCFEQEEELTIENVKKYSRLHKSCGTSFLFIVVLV
                     SVITFIIFNVENFWLRLVSRVVLVPLIAGISYELIRWARRSKSKAAQMISIPGMWLQK
                     VFTTREPDDDQIEVAIAALKGVLEDEEDH"
     gene            complement(31638..31847)
                     /gene="rpmE"
                     /locus_tag="PLG49_00140"
     CDS             complement(31638..31847)
                     /gene="rpmE"
                     /locus_tag="PLG49_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOQ15751.1"
                     /translation="MKSDIHPNYGEATVICNCGNTFKTGSVKKEIRVEVCSNCHPFYT
                     GRQKTTAAKGRIDMFNKKYGINQEQ"
     gene            complement(31985..33289)
                     /gene="rho"
                     /locus_tag="PLG49_00145"
     CDS             complement(31985..33289)
                     /gene="rho"
                     /locus_tag="PLG49_00145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOQ15752.1"
                     /translation="MENEYNNKTLAELREIAKDLGVKNITKYKKQELIEEILTKENDT
                     ENNSSEKTKVPSSIEQLDSGIVEEGVLEVLVDGYGFLRSENYLPGTKDIYVSPSQIRR
                     FNLKTGDLVKGNIRIPKEGEKFSALLYVKEVNGDNPEVAAKRPNFEDLTPIYPTEKLH
                     LEYDQLDLSTRLIDLIAPIGKGQRGMIVAPPKAGKTVLLKKIANAIVKNHPEARLIVL
                     LIDERPEEVTDMKNSISGENVEVVYSTFDEQPEHHKRVAEMVLERAKRLVEQKKDLII
                     LLDSITRLARAYNLTIPPSGRTLSGGLDPAALHMPKKFFGAARNIQEGGSLTILGTAL
                     IETGSKMDEVIFEEFKGTGNMELVLDRKLSEKRIFPAIDIYKSGTRKEELLLKQNELE
                     VVYGIRKAMSRMTPAEVTESIIETLMHTKNNEEFIKLIEKKL"
     gene            complement(33308..34192)
                     /locus_tag="PLG49_00150"
     CDS             complement(33308..34192)
                     /locus_tag="PLG49_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ15753.1"
                     /translation="MEIKDWIEDDEFENLANLDETEKKKILDELEELFNKPASFDNNE
                     ENHNNFLRPSIKSKYRSRRKKKNIIMDKIITSAIVMGSVVLIAAVIWGGYSLVRLFFN
                     NDEDILFVEDNVSSEQVALLQSEINELRKKNQELIAENNELRQKLQELRTVVDIELER
                     LKVQIQEESSYEDNNSPQSNSDSQTSSINTSNTNQTKNNLTANQNSQGNQTSQANQAN
                     RTNQTNQASQNNSSDNTQSNNDDQFTYYTVKQGDTLWSISMDFYGSGQYYKKIMQYNN
                     MRSERELYAGKRLKIPKL"
     gene            complement(34304..34924)
                     /locus_tag="PLG49_00155"
     CDS             complement(34304..34924)
                     /locus_tag="PLG49_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15754.1"
                     /translation="MLINELQTIVFQCAACGGYCFDIISLFDFSGNKDEKIQCKCSRS
                     SVQLSLNDKKQIILSVPCFLCSDIHTYTYKINQFFSDSLKILQCPKKNMKIGFIGNDT
                     KVRSALDSHENSLNEILKEMGMPNLCNNVEVAIESINYIHDLAEQGRISCECGSNKIE
                     MELLANQVKLSCYFCGSETVLLTRNNEDLKYLQSQPNILLKKMQVY"
     gene            complement(35014..35850)
                     /locus_tag="PLG49_00160"
     CDS             complement(35014..35850)
                     /locus_tag="PLG49_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ15755.1"
                     /translation="MKIKIVADSSCDLTTEMKKDMNIKIVPLTLQLGDKIYIDDENLD
                     IKEYLTAMKEYEGSPKTACPSPEQFMKSYEGDESTFVVTLTSALSGTYNSAVLAKDMF
                     LEEISDKFIHVFDSLSASIGEVLVCLKIHELSKLNIGDMEIVKRVNHYIKEMKTFFLL
                     ESLEHLAKAGRLNPIIAKLVSVLSIKPIMGATEEGTIRMVEKIRGYKRAFDRLVSIIG
                     EEGKNLEEKILGIAHCNCIERALKFKEEVMKRYNFKDIIIVEMNGLSSSYAGEGGLVI
                     AF"
     gene            36335..36526
                     /locus_tag="PLG49_00165"
     CDS             36335..36526
                     /locus_tag="PLG49_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOQ15756.1"
                     /translation="MIAKIDRDGCIGCGTCPDVCPEVFRMADDGLAEVYVDEVPEDAK
                     EAAIEAQEACPVSVITVEE"
     gene            36667..37551
                     /locus_tag="PLG49_00170"
     CDS             36667..37551
                     /locus_tag="PLG49_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOQ15757.1"
                     /translation="MNSIKKVLYSIIFISMVLYAMLPNKPDIARYETFDDTPLITFYK
                     DETIDEAINVSAQTYPYLYAKGGIVLDRNLHLWQVGEDVLQIKKALKKIGYPISTNSY
                     IFDENMQKIVMQFQSDHDLNSDGIVGKATLNAINKILIENKMTAPTVQTIVTNAPTSG
                     YWIAINKSSNTLTLFKGEQVIEKFPVATGKEPKLTPEGKWKINNKVINPAWKNIKGGV
                     PENPLGYRWLGLNIGDGYIYGIHGTNNENSIGYYVSKGCIRMENEDVNKLFSIVKNGT
                     PVWIGNQKQLSQWGIVIK"
     gene            complement(37571..38116)
                     /gene="yfcE"
                     /locus_tag="PLG49_00175"
     CDS             complement(37571..38116)
                     /gene="yfcE"
                     /locus_tag="PLG49_00175"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOQ15758.1"
                     /translation="MKLFFLSDIHGSVYYAKKALDIFQKEGADYLILLGDQLYHGARN
                     PLPREYNPKELAALLNQMKDKIIAIRGNCDSEVDEMVLDYPIMATYSNILYYNRRLFI
                     THGHLYNENNLPELCKGDVFVYGHTHIPVAKKEGDIYIMNPGSITFPKEDSPNSYAIL
                     EDNLFKIKDLEGNIFKELLLE"
     gene            complement(38185..39186)
                     /locus_tag="PLG49_00180"
     CDS             complement(38185..39186)
                     /locus_tag="PLG49_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15759.1"
                     /translation="MQGHKRKVFIFLMILVMIPVFFVMHKAEANRTLKAQWIKDYIEG
                     KDQINNKENILEIVLKDTNYTEWHDKKDKIGLNILKADLLGDSKKELLLTLFLAPKKS
                     LIVVYQQENGDYKYVDILDEFFEIKEIQAIPIEKRGKDLIVVREFVDQMLGAFEKGNY
                     IRGYLWQDGKFNMVISLIENYVAYWNESWDQAKKPNPLWLKIEEKSIIQWENGPYPVI
                     KVLENQFFYQSKTANLKEMPEEEDYQLVNSKELVQTYYWNEKYQHFLIAEGKDVKTGE
                     SIGIIEDLSLSPFQLAGFEKNQYRIKRKNGKIEFVPKSQIIRTDKNNPLRKSEDFVI"
     gene            complement(39286..39714)
                     /locus_tag="PLG49_00185"
     CDS             complement(39286..39714)
                     /locus_tag="PLG49_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15760.1"
                     /translation="MQLRNCRRCGKLFNYVGKPICNECVQQDEKDFELVKAYIYDHPK
                     ATITEVSKETGVSVKKITKFLKEERLELAEDSPAWLTCESCGVEIRTGRFCKECSMRL
                     KNNIGQYSSLDKDKNKEDAIDIKRTNYNSKMHLRHHLEKD"
     gene            complement(39806..40822)
                     /gene="trpS"
                     /locus_tag="PLG49_00190"
     CDS             complement(39806..40822)
                     /gene="trpS"
                     /locus_tag="PLG49_00190"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOQ15761.1"
                     /translation="MTAENQKDKKVIFSGMQPSGNITLGNYLGALKNWTKLQDEYNCV
                     FCIVDLHALTVRQDPAELRRRSREVLMLYIAAGLDPEKNIIYYQSHVGAHAELAWILN
                     CYTYMGELNRMTQFKEKSQKHSENINAGLFTYPVLMAADILLYQANLVPVGNDQSQHL
                     ELCRDVAIRFNNLYGETFIVPEGYFPKVGARIMSLQDPTKKMSKSDENENSYITLLDS
                     PDTILRKIKKAVTDSEAKIAYRDEQPGIKNLLNIYCSITEEPIEKVVERFSSLGYGEL
                     KAKVAEVVVEELRPIQERFYQLEKDKSYIDSIIKNNAERASRIAYKTLSKVQRKIGLP
                     AKIR"
     gene            complement(40920..41435)
                     /locus_tag="PLG49_00195"
     CDS             complement(40920..41435)
                     /locus_tag="PLG49_00195"
                     /inference="COORDINATES: protein motif:HMM:NF028409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HOQ15762.1"
                     /translation="MKVKQLTTSAILLALGAILHAVVPGTFAGLKPDFLLSMMFLAIF
                     INFNPTNIAATSIACGIISAMTTNFPGGQLPNIIDKCITGVVVGLMIYALKKLSVPES
                     VKMGIIGFIGTLLSGLAFLGSAALIVGLPGAFGTLFAGVLFAAVGNVIFVVLLHQVYI
                     RAGLNKFTVSQ"
     misc_binding    complement(41497..41759)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41834..43219)
                     /locus_tag="PLG49_00200"
     CDS             complement(41834..43219)
                     /locus_tag="PLG49_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOQ15763.1"
                     /translation="MRKSRNIIIAALVLVILLGAYLYINHQSQENGIEEETDSELTIS
                     NTSRDKITKMILTSSKGEITIERKDDIWVLTGNEDIELDQDKVNDLAYSFAVIFAERI
                     VEENPQDLEKYGLKSPVATVKAFFDDGDTREYYLGNKTPEGTTYYLMKKDDPKVYTVW
                     MNHGENFSSGLDDLRNKKLTAINTLELDYLYIKQEDKPVIEIVSNINEELNKYGLDLW
                     MLKQPYNHTYAVGSKDFMELLEKGFEFTIEEFVEDKPSDLSKYGLDEPRIEIQIKDTS
                     NSLHLLVGSNKDDNLVYAKTADKDTVFTMAFSKLQPLLDLKPIDLIDRFVYIIDINLI
                     DAIEIEHEGKVDKLTLSRTTKKSEDSDEEETITTYKVNGKEVEEEAFKDYYQNLIGIT
                     FDAELDKEVEKTEPEIRTTFTLNSGEKVTVEYVPYDDDFYTVYRDGVSDFVASKKKVK
                     TMLDSLKELTK"
     gene            complement(43216..44661)
                     /locus_tag="PLG49_00205"
     CDS             complement(43216..44661)
                     /locus_tag="PLG49_00205"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOQ15764.1"
                     /translation="MKNNIWESFKDKRVRYGGYASMMTAFVVAILIIINLMVGQFDYK
                     LDLTENKMFSLSQQTLELLDRLDQNITVYVLTETGKENLIFDETLKQYSAHSNKIKVE
                     YKDPVLYPQFATKYDKEGKGISNDSLIVEGENGKFKIIPPSQLFNYSYNSGSYIVESL
                     AVEERVTGAIQYIISEKNPVIYTLKGHDEPSLPYYVKTQLEKENYEIKDLNLLTDKEI
                     PEDADILMIYAPVRDISKEEGAKITEFLENQGRAIILTNVLTNVKEDQMPNLQEVLKK
                     YGLSIRKSMILEMDAKYQFANPLYIIPNLQGHEITNPLKSSGLPVLVAVAQNIETLQV
                     KRDSLEISPLLTSSETSYAKTNFQSEVAEKEEGDIDGPLDIAVAVTDTWYENNEEKIT
                     KLVVVGNTEFLNETINAQSAGGNIDFFMNTVNWLVDKEESISIRPKSLLTTRLKINVS
                     QVYIYAGITVVLIPLIIGIIGLTVWLRRRNR"
     gene            complement(44676..45539)
                     /locus_tag="PLG49_00210"
     CDS             complement(44676..45539)
                     /locus_tag="PLG49_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ15765.1"
                     /translation="MLAIINKELRNYFASATGYIFMSFFLLISGIFFSITNLFEQNPV
                     YNNVLSSITFIFLFVVPMLTMRLIAEETRQKTDQLLLTSPLKVSEIVLGKYFAALLLF
                     LLTLFITVLYPVILSFHGKIAVGEIVGGYIGFFLLGAAFISVGLFISSLTDNQVVSAV
                     VTVITLLVLWIMEGIIMGLPTDRKSSIVFMAILVIAIAALFYNSTKNIFISAAIVIIG
                     AIIMVAVYLINPALYDGVIVRIFQWFSLLERYQSFSYGIFDVSSVVYFITFSCAFIFL
                     TIQTIEKRRWS"
     gene            complement(45523..46563)
                     /locus_tag="PLG49_00215"
     CDS             complement(45523..46563)
                     /locus_tag="PLG49_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ15766.1"
                     /translation="MIEVINLTKQYGQHKAIDNISFTVEKGEILGFLGPNGAGKSTTM
                     NIITGYISATEGTVKVDGYDILEQPEEVKKRIGYLPELPPLYMEMTVEEYLKFVCHIK
                     KVKPQKQKEVLGKVMNAVKITDMRKRLIKNLSKGYRQRVGLAQAMIGEPQVLILDEPT
                     VGLDPKQIIEIRNVIKSLGKDHTIILSSHILSEVSAVCDRVLIINKGKIVAIDTPENL
                     AKRLSQSNKMLLRIKASKDKALNIVKGVKSVKTAEITGSREPNTIDIVVEGKEGLDIR
                     EDLFYAFKSTDYPILMMKSLDLTLEEIFLQVTTQEQAIDHESISISEENDAQHEETDG
                     QQREDGEDVSNN"
     gene            complement(46833..49961)
                     /locus_tag="PLG49_00220"
     CDS             complement(46833..49961)
                     /locus_tag="PLG49_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ15767.1"
                     /translation="MKLSKMSIQRPVTTVMLILIAVLLGTISIRRLPVDLLPQFSLPY
                     AIVMTQYSGAGPHEIETLVTRPLEGAVATVSNAKNITSISSNGSSIVMVEFNDGTDMD
                     MAMLDMREKIDMIKGYLPEDADEPLVLELDPNMMPIMQIGISGNKDLEALTRTVQNNV
                     IGKIEKIEGVASVSLSGGQEKEIRVTLLPDKLKGYNLTPASIAQFLALENLNLPAGEV
                     KQGKTTLTLRSIGEFNNIDEIRNLPITTSGGIIYLRDVAEVEEVYKKMNSYAYINGNP
                     SISLSVQKQSTANTVKVSRAIMEEISQLKEDPDLKDIEIRIIYDSAEFINMSVENVAS
                     TAIIGGILAIFILFVFLRNIRSTFIIGLSIPISIIVTFAFMFFVDLTLNIVSLGGLAL
                     GVGMLVDNSIVVLENIYRHREKGKSRVDAAEIGTSEVGLAVTASTLTTIVVFLPIVFV
                     EGWAAKIFKEMALTVTFSLIASLIVALTLVPMMASKILRVEKVDEIKRNKLITKILDK
                     WAAGLNKVDVFYKRVLNWTLHHRKRAVGITLTVFTATLSIPIFGLVGMEFFPATDQGQ
                     FTINIELPKGTVIEETFEVVEKIQTRIEEIKEIQEVFVNIGGGAGLLGTETASNSASM
                     TINIGSVKNRKRSVDEIADEVRNLTKDIPGAQISVTGSSFMAGLSGSSPISIDISGDD
                     FEQLEVIANDFVEIVRSVDGTREVSHSVEEGTPEAQIVINRSKASMYGFNMTTISNAL
                     RTAVQGSVATKYKVNGTEIDVRIQYDSSQLEYLKDIKDISVTSALGVSIPLSEIADIS
                     INKSPVSIMRDNQKRMVTVSSSVFGRDINSVKKDIEEKLKKYHMPEGYNYEFSGEIEQ
                     MMSSLESLSLALILAVILVYMVLASQFESFLHPFTILFSVPISLTGAILALFITRRTI
                     NMSSFIGFIILVGVVVNNAIVLIDYIIQLRSRGYDRTEAILEAGPTRLRPILMTTLTT
                     VLGMIPMSLGLGEGGEITAPLATAMIGGLSLSTILTLVVIPLNYTIFDDIAEKLKKLF
                     KRNKKQIQQKSL"
     gene            complement(49980..51254)
                     /locus_tag="PLG49_00225"
     CDS             complement(49980..51254)
                     /locus_tag="PLG49_00225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ15768.1"
                     /translation="MTIKKLTSVLLLPVFLLSACSNTGGNNEIEEKVTPVYIQKVSKG
                     QISDTFTYGGKINPKQQITVSSKVAGKVKEVYFDIGDYVKEGVVLFQLDETDLQNNIK
                     ALEAQLKSAQATVNMGQIGLSSAKGSQYKQQKSQMETALKNAEIQLQDSKKAYEDMKV
                     LYEMGSVSKQQLDQSESAYQMALLAYQAAKNNYDLFMNNLSKESVQLAQNQLEQAVAA
                     KESLEVQIANAKEALNDTAVRSPIDGIVSSRMIDAGEMVAGAIAPFTIIQIDQVFVDV
                     YVSEQIINKIEKNQKVDVYVRSASTDKFEGKIAAISPAADERTFTYLVKVEIDNQGGL
                     LKPGMFAEVEFVLDSANNVIVIPREAILTEGDRDFVYIVEGDRAKKIEVKLGLDNGKE
                     AAILEGLKEGMEIVIKGQEYLLDGGKVQIVEQ"
     gene            complement(51290..51751)
                     /locus_tag="PLG49_00230"
     CDS             complement(51290..51751)
                     /locus_tag="PLG49_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ15769.1"
                     /translation="MDHKKTKEVSLQFLNLIIHLTHWLHGKYFKEFNKNGKTNRIITD
                     RQFSILILVYQLKLCTLSDLQKELNVSSSSLSIGVSKLVKEGYLTRTYPSEEEDRRKI
                     YLSITPKGADVLMEVHKNVLFVFEEFYENLSDDKKKDFEEGINKLKKVFNQ"
     gene            51947..52426
                     /locus_tag="PLG49_00235"
     CDS             51947..52426
                     /locus_tag="PLG49_00235"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOQ15770.1"
                     /translation="MKKIASFTVNHLKLLRGIYVSRKDALGSEIITTFDIRMKVPNKE
                     AVMNTAEIHTIEHLGATFLRNHEKYGNQIIYFGPMGCRTGFYLVIGGDFSSETILPLI
                     REMFEFIANYDGDIPGASPEECGNYTDMDLKTAKFEAKKFLEEVLLNIKKENLNYPD"
     gene            52505..52687
                     /locus_tag="PLG49_00240"
     CDS             52505..52687
                     /locus_tag="PLG49_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15771.1"
                     /translation="MATFPNPFFRKFTYPKNSFLRISSLNKIFPHKNHVLFNYDEEYA
                     KENNEDFKNNQEKIPH"
     gene            complement(52699..55089)
                     /gene="pheT"
                     /locus_tag="PLG49_00245"
     CDS             complement(52699..55089)
                     /gene="pheT"
                     /locus_tag="PLG49_00245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ15772.1"
                     /translation="MNIPMSWLKDYVPVDCDIKTFADAMTMSGSNVENYVEQGKEISN
                     VVVGKILSVEKHPDADKLLVTKVDVGTEVLQIVTGAHNIKEGDYVPIALHGASLPGGI
                     KIKTGKLRGVESQGMMCSINELGFEKEDFPDAPEDGIYILSEAYPLGKDIKEVFGLND
                     IVVEYEITSNRPDCFSVVGIAREAAATFKLPFKYPEINVKESGGKASDYVSIEIANSE
                     LCPRYAARIIKNVKIQPSPKWMRQRLLAAGIRPINNIVDITNYVMLELGQPMHAFDYD
                     KLKNKKILVRNAKAGEKIMTLDGETRDLDETMLVITDGENPIAVGGVMGGEESKVTED
                     TKTILFESANFNGANIRVTAKKLGLRTDASVKYSKGLDPNLVIDVLNRAAQLVIELGA
                     GEVVEGIVDVYPNKREAKTIPYSVEKINGLLGITLSEDEIINYFTSLEFKVDREAKTV
                     TIPTFRPDIEGNADLAEEVARLYGYDKITPSLERGKPTVGKKTYSQRIEDLIKITMEA
                     QGFYEIMNYTFESPKVFEKLNISEDSDLRKVITISNPLGEDFSIMRTQTLNGMLTSLS
                     INYNRRNEEVLLYEIGKVYIPKELPLKELPEEKLKLTLGMYGNVDFYDLKGSIEVLFE
                     KLGMSEKVEYSLEQNLSWMHPGRCASISCKNKKIGYIGEVHPNVAENYDINTRVYVGV
                     IDVSSLVEEADLERVYKPLPKYPAVSRDIAMLVKDEIPVKDIENIIRQRGGKILESYS
                     LFDVYKGKQIQEGYKSVAYSITFRAQDRTLTEEEVNKAMQKIINGLESNLGAHLRE"
     gene            complement(55104..56123)
                     /gene="pheS"
                     /locus_tag="PLG49_00250"
     CDS             complement(55104..56123)
                     /gene="pheS"
                     /locus_tag="PLG49_00250"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ15773.1"
                     /translation="MKEKLQNIRNQAISLLNDVKELKALDEIRVEYLGKKGQLTQVLR
                     GMKDLSPEERPIIGQLANEIRDEIEQKLEEVKNLLILKEQEKKLKEEVIDVTMPGRKR
                     KTGGRHPMNLVLNEIVEIFLGMGYKIAEGPEIETDYYNFEALNIPKDHPARDEQDTFY
                     INQEFMLRTATSPIQVRVMEKQKPPVRIICPGKVYRSDAVDATHSPVFHQIEGLVVDK
                     NITFADLKGVLEVFAKELFGEKVNVRFRPHFFPFTEPSAEMDVSCVVCGGKGCNVCKG
                     SGWTEILGCGMVHPNVLRMAGIDPEEYTGFAFGMGLERLAMSKYGIKDLRLFVENDIR
                     FLKQF"
     misc_binding    complement(56176..56425)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(56496..56747)
                     /locus_tag="PLG49_00255"
     CDS             complement(56496..56747)
                     /locus_tag="PLG49_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15774.1"
                     /translation="MENRLVRMLVYLVIGVVTVVFGVPLLFKAIGLLFKLIGLSFSLA
                     FGLLALVISALPFILIGCVIYHLYFKKNSAVTNSNTNTN"
     gene            complement(56859..58028)
                     /locus_tag="PLG49_00260"
     CDS             complement(56859..58028)
                     /locus_tag="PLG49_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ15775.1"
                     /translation="MSILLYLGIMLLAGLLFGRLVKFIGLPNVTGYLIAGLLMGPYVF
                     NILSFETVEHISVVSEMALAFIAFSIGSEFSVSYLKEMGIIPIVIAFFGAILTAVFVA
                     LGLIIYGLDPALAVMLGTIASATAPAATVMVIKQYQANGPVTKTLLSVVAIDDAIALI
                     AFGFALTFINSSNEAGGSVLLSLLSPFIELTLSAALGLILALLLLIPLHFFKKESNRM
                     CAIIGILFITSSLADYLGASALLACMFLGAVLVNIEKEANLIFKIADSITPPVFMLFF
                     VLSGAELDVSIIPSIGVIGVIYILMRIFGKVFGAWFGAVLTKSVSTVKKYVGWTLLPQ
                     AGVAIGLSLVVDKALPQYSSQVRAVVLCSVLVFELIGPIASKIALTKAGEIEYKS"
     gene            complement(58025..58381)
                     /locus_tag="PLG49_00265"
     CDS             complement(58025..58381)
                     /locus_tag="PLG49_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15776.1"
                     /translation="MKLFVLILNRTEKLNDLMMTFLKEGIAGATILESTGMVRELYHF
                     KHDEEEINFLGSIRRYLSGDENKKSKTILTVIRDDQEETVVRITEEVVGDFSAPDTGI
                     MFTLPLDFVRGKGLDK"
     gene            58572..59639
                     /locus_tag="PLG49_00270"
     CDS             58572..59639
                     /locus_tag="PLG49_00270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ15777.1"
                     /translation="MLNNFLNKMLLITSILTLGSILFKKYLFQQNKYKKEFLGFFGGT
                     IGILFFAYPDLIHDNALYIGTLWIILSATSLFGNFSSSLIAGLMLLCFDLIYFMSLDI
                     TIISLLFILICGIADILPIRQKYKWLVLNSSWFLIFLSYSKSLSIPVYPFLMICITAS
                     SYVLLLLYLIRKNYISYMNLKIQATKDYLTGLNNKRQFNLILKKTFSNLGKKDKLALI
                     MIDIDYFKYINDTYGHPTGDKILKQFTEVLKKLCPTNNISRLGGEEFGILLSNYSLSK
                     TYDLAEKIRSGIQESPFKIDEENTINITVSIGISVYPDTAEDISSLIKTADQALYKAK
                     KFGRNRVNLPLKIYNIKEPAS"
     gene            complement(59677..61074)
                     /gene="gltA"
                     /locus_tag="PLG49_00275"
     CDS             complement(59677..61074)
                     /gene="gltA"
                     /locus_tag="PLG49_00275"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOQ15778.1"
                     /translation="MPNMSLNKVKMPEQDPQVRNQNFDEVALGYTKEQAMEEATRCLN
                     CKHKPCVSGCPVNVPIPEFIKEVTQGNFEKAYELITKENNLPAICGRVCPQENQCEKN
                     CVRGIKGEPVGIGRLERFVADYHMQQGKKSSPDIKKNGIKVAVVGGGPAGLTCAGDLA
                     KLGYDVTIFEALHTLGGVLMYGIPEFRLPKKLVEAEIQNIIDLGVKIEKNVVVGRSVT
                     IDELIEEGYKAVFIGSGAGLPKFMGIEGENLNGVYSANEFLTRINLMKAYDFPNSPTP
                     VKVGEKVAVIGGGNVAMDAARTAKRLGAKEVYIIYRRGEEELPARKEEIIHAREEGII
                     FKFLSNPKKINGENGWVKSIECVEMELAEADESGRRRPVEKKGSNFTVDIDTVIVAIG
                     QGPNPLIRQTTPGLLTKEWGGIVVHEETMETSKENVYAGGDAVTGAATVILAMGAGKK
                     AARAIHQKLQEDKSK"
     gene            complement(61076..61900)
                     /locus_tag="PLG49_00280"
     CDS             complement(61076..61900)
                     /locus_tag="PLG49_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOQ15779.1"
                     /translation="MYKILEKEKLNDLVELMVIHAPFVARKCEPGQFVILRVDEKGER
                     IPLTIADFDREKESVTIIYQILGYSTKLLSEKKVGDCLQDFVGPLGKASELKKHKRVL
                     GIGGGVGAAPLYPQIKKLAEMGVEVDVILGGRSKEFILLADKFEAICNQVYFATDDGT
                     KGKKGFVTDLLKELLNSGNKYDEVIAIGPLLMMKAVVAITKPEKIKTTVSLNPIMVDG
                     TGMCGGCRVTVGGEIKFACVDGPDFDGFLVDFDECIRRQGMYKEEEHICRIGLGGV"
     gene            complement(62156..63775)
                     /locus_tag="PLG49_00285"
     CDS             complement(62156..63775)
                     /locus_tag="PLG49_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOQ15780.1"
                     /translation="MSLNFYQEDQSFNSQSSNACMELAIEGANDGFWFWDIKNNKIYY
                     SKKLNELSDISGSSHYVFYDAINHIIHPQDIDGLSHFLNKYLKKEIQHFKIECRIKFN
                     TAYEWVLIQGKAKWDEDDEAIHMAGSYTFLTDLNNYNETQKMLRETVAMLNVIFNNLP
                     DPICIKDKTDKIIQCNPAALIFWKQSQEEIVGKKCNSMCISPCQETVTQKSLKYKKPY
                     KVQKYIETMKKWVDIMAYPVLDDENNVQFLIEHIRDITDLKRSEMILQKHIGLQERLL
                     EEANEYDKLKTEFFANISHELKTPLNVIIASLQILEKYYTPADNGTKYTKIMKQNCYR
                     LLRLLNNLIDMTKIDTGFVRLHLENKDIVSFIKHITMSVAHYIENKSIKLSFNSTKDK
                     IIMAFDSEKIDRIMLNLLSNAAKFTPSGGEIRVSISEENNQAVISVKDTGVGIPTEKQ
                     KLIFERFGQVDSPTTRHNEGSGIGLSLVKSLVELHGGTINFYSEEDKGTEFIIKLPIK
                     TIDEDKATEKSQNLIINNEENLYMELSDILG"
     gene            complement(64040..65371)
                     /locus_tag="PLG49_00290"
     CDS             complement(64040..65371)
                     /locus_tag="PLG49_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOQ15781.1"
                     /translation="MKNPINVYKLRKLIVGINRKVPLMDDRSGIEINFDNGATTPPLL
                     PVVEAVNRYLPYYSSIHRGVGYKSRVSTWIYEKARERVADFVRADFNKDVVIFTSNTT
                     QAINKMARRLFQKEGRGLVLSTAMEHHSNDLPWRSQFDLDYIGVDQYGRLDIKDLEYK
                     LEKYKGKVKLVTVTGASNVTGYINPVYKIAKLAHQYGARIFVDGAQLVPHVSVDMKAH
                     DDPEHIDFLAFSGHKMYAPFGTGVLIGHKDFFEGGRPSEPGGGTVKFVSHKDVLWNDA
                     PLNEEAGSPNAVGVVALAEAIKFLKKIGMENIYQYETKLSDYAIRRLREIDDIIIYGE
                     AFQKSGRVGIISFNIKGLPHEVTALALAYEGAISVRSGCFCAQPYVQKLLDISEEESK
                     GYMKNDKGRPGLVRISFGFYNTFQEIDQLIRLLKIISKNKSYFIKKYGISE"
     gene            complement(65508..65969)
                     /locus_tag="PLG49_00295"
     CDS             complement(65508..65969)
                     /locus_tag="PLG49_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOQ15782.1"
                     /translation="MNKYTKFLTQAAAIAAIYVVITLTFGFMGFGPVQFRISEALTVL
                     PYFTPAAIPGLFIGCLISNLVGSSLLDIIFGSLATLIAAILSYKMPKKFLVPLPPVLV
                     NAIVVPIILKITMNAPFLMSIVTVGLGQVLACYGIGYPLILLLDKNKQKIF"
     gene            complement(66071..67843)
                     /locus_tag="PLG49_00300"
     CDS             complement(66071..67843)
                     /locus_tag="PLG49_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ15783.1"
                     /translation="MTVNKDDKITQKSNIEIIMRLFAYLKPYKLEIAETLFLMSIVIA
                     VELLNPYFMKRGIDEYIVQKDIKGLFLLGGIMIIANILALVCSRKRIRIMAKVSNKII
                     LTIRQELYVHIQSLSFSFFDSRPTGKILARIIGDVNSLNDLFSNSVTNLIPDMIKIII
                     VVMIMLSMNFQLALLSLTTLPFLLLSMFCIQIVSRKRWQTYRKKNSLMNAYIHEDFSG
                     IKVIHSFAAEEYTSKRFYTHLNDLKTAFVHAVKMNDLFWPITEFSWGLGSIIVFWFGV
                     SMLNSGDITVGLLVAFTGYISIFWRPIVNISDFYNLLVINMAGAERIFNIMDIKADIK
                     DMENARVLPEIKGDVVFENVSFSYDDDNIVLNDVSFQVKAGETIALVGPTGAGKTTIV
                     NLVSRFYEAQKGRVLIDGYDVKEVTLESLRGQMGIMTQDTFMFSGTIKDNIRYGKLDA
                     SDEEIINAAKAVSAHDFIMKLEKGYDTDINERGSRLSVGQRQLIAFARALLANPRILI
                     LDEATASIDTQTERLIQKGIERLLAGRTSFVIAHRLSTIQNADRIMVIDEGKIKEMGT
                     HRELLEKKGLYYDLFKAQFNFLND"
     gene            complement(67853..69595)
                     /locus_tag="PLG49_00305"
     CDS             complement(67853..69595)
                     /locus_tag="PLG49_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ15784.1"
                     /translation="MRRLFRYVNKYRKIYLMGFIVMMLNLILDMFNPYFSRIVVDEVI
                     VEGHLEKFKGALLGLLIITVTRGILGYVKDMTFDWVSAKTIVDIRKDLFDHIQSLSFS
                     FFDKMNTGELMSRIKEDTDNIWQAVSFGIMLFLEQIFYFIIASCLLFILHWKLALVSL
                     SLMPLIGFFAYKFERKAGETYEKISDQGAVLNTTAQENIAGVRLVKAFGREKYEIKKF
                     LIENKKNYELNLEQASVLAHYNPMIEFLSNLVIVIGIVAGGLMVIREKISIGLLVAFS
                     NYIYMLIWPMRLMGWLTNLLARALASSKKIEKIFNEEALIKNPENPVVPEKIKGNITF
                     KNVDFEYDGHKALEDISFELKAGNTIGIMGETGSGKSSLIHLIGRYYDCTRGSILIDG
                     IDVKEMDLKTLRRNISVVMQDTFLFSDTIEENIRFGNREASKEDLIKAAIDAEVHSFV
                     KEMPQGYETLIGERGLGLSGGQKQRISIARALVKNSPILILDDATSALDMETEYKIQK
                     ALEKRKDITKIIIAHRISAVKDADEILILEDGKIVERGNHKSLLDKKGKYYEIYCEQF
                     KGLKEISELETRKV"
     gene            complement(70052..70480)
                     /locus_tag="PLG49_00310"
     CDS             complement(70052..70480)
                     /locus_tag="PLG49_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ15785.1"
                     /translation="MKQKILVCITIQQNSRRLIKKGAELAEQLGGELHILHVEQGNNI
                     LSKNDTGELLQELFDYASKLGAEVHVVCDDNVPERIVSFIREHNITELVLGETMKNKI
                     YRLLTNDIASYIASTTPEVEVIVLEREKKLNVKDQEAFSS"
     gene            complement(70553..72364)
                     /locus_tag="PLG49_00315"
     CDS             complement(70553..72364)
                     /locus_tag="PLG49_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOQ15786.1"
                     /translation="MERRRSMNWASYAQRLIYENGEYHLIIYLPEDAEFAKEFGELSL
                     EKEKNNILSYIKTAYPAVKIQSVKFMAGSIITATLMLHNPTTKVNAASSSFNQTKSIQ
                     LRINNRYVQSDTPPVIENGRVLVPVRIVGESLGAYVYWDSKEKVAIIKKGDTTIRLQA
                     NSLKAFIDGKTHFLDAAPKIINGRLMVPIRFVSEAFKIPVDWNHEAKVVLINSSLPKV
                     SDYVVQKGDTLYGIAIKHKITVEELKEWNQLKSDVIYVGQLLRVVPPSIIIEDPGSPL
                     EEMEPIAYQFDTVLGFTVKYYEGHLSSYNSLKSNHQKITDVVTFSHGIKTDGSLESDY
                     DQGEILAYSREKKIDSSMLIHNAKGGSFHKELAEEVLEDPILRNQLVKNILTEIEKSG
                     YKGVTIDIEGIGPDVRAEFSSFIKELKAELGPKKYILSVCVPAKMADHPKDNWTGAFD
                     YKTIGQYADRVIIMTYDEHWSGGSPGPVASYDWVENVINYAVGEISPKKILMGIAAYG
                     YDWPIEGKNGKAVTVSEINDYLKKYGGKILWNDTFKVPYYRYKDESGKERIVWFENLQ
                     SSQYKMQLAKKKGLEGIAIWRLGLENKEFWDGIKNKQ"
     gene            complement(72431..73321)
                     /locus_tag="PLG49_00320"
     CDS             complement(72431..73321)
                     /locus_tag="PLG49_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOQ15787.1"
                     /translation="MNTLKRISRVFETAEEILFDDSSKIIIMSDCHRGDGSWADDFAR
                     NQHIYFYALSYYFNKGYTYIELGDGDELWENSDMTEIVQTHSHVFWLLSKFYREGRLY
                     CIYGNHDIVKRSEKFVEKKLWEYYDEAQKRHVPLFKNIKFHEGLILKHKITGKKIFLL
                     HGHQGDLLNDQLWFLARFLVRYFWRPLNVFGIRNPTRTAKNYYKKIAVRKRLTKWVEK
                     EKHILIAGHNHKPSFPELSESPYFNDGSCVHPRCITGIEIIDGYIMLIKWHVKTRDDG
                     TLFITREILDGPRKLNDYKG"
     gene            73512..73835
                     /locus_tag="PLG49_00325"
     CDS             73512..73835
                     /locus_tag="PLG49_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ15788.1"
                     /translation="MAIDDTSIIDWIAMSQDEQNLVMLITDHLDRDEDFEQTHLFLLQ
                     EKINVYLAFIDSREYIEVYPGKTFNHFVIEVHFLNAMSQNCKNFIDTVNRELEKFNIR
                     IEAFN"
     gene            73997..75286
                     /locus_tag="PLG49_00330"
     CDS             73997..75286
                     /locus_tag="PLG49_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15789.1"
                     /translation="MKIIKENEQLILQSSLINKLINSLVVLGIFLFVSFMIFKNMHSF
                     DLLVLIFPAIYLILFLFVFFKKDLYQCIFDKDHKSITIIWRNFFFSHKKYFSFNDLIA
                     LQIDKRSDDDYNDYILYIVFSEQKIFVHSARQRSIVEDMANAIINFVDNPALKINPAT
                     IIYESHTSNTNNMPAFYKYYSKGREGKFTGSLFVKKVSLKRTDNKLILKIRKSFLSVL
                     AFLLLLFFFFIFTKDILTIESITFNKINNNKITCSIKNYLLGNENMEISSLHLEHFKD
                     VRLDVDDEGGSIFIILYDKNLEYSSINFDQKAFEQVKNFIEDKEETELILWESFSFFD
                     LFWFLLLAFLIIAYMFIPYISCIIIDKDNFIIQIKYKGFRMNKHEKHSIHVINSISYN
                     ESGKQYYLEIELFGKKIELGVLTDKEKISEIIDFLNS"
     gene            complement(75290..75697)
                     /locus_tag="PLG49_00335"
     CDS             complement(75290..75697)
                     /locus_tag="PLG49_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOQ15790.1"
                     /translation="MMSSLFQFLLSTKSSQEFLDITHLLKQAIEKTGIKEGIAVVYCP
                     HTTAGITINENADPDVVRDILVTLDKVFPVKGDYRHFEGNSHAHLKSSYVGAEKTIII
                     HEGKPVLGTWQSVYFCEFDGPRTRKVFIKIIEG"
     gene            complement(75812..76645)
                     /locus_tag="PLG49_00340"
     CDS             complement(75812..76645)
                     /locus_tag="PLG49_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ15791.1"
                     /translation="MKDRIADLHIHSYYSDGTMSPKKILSVALKKGLGLLAIADHNVL
                     EGSKELQALCQGYDIKFLSAVELDSLDEGEDIHILGYGMNFNDKAFCQFVQRNRFLLD
                     EISVKLISEMEKDFDNISLEDFLSFNYDRTKGGWKALHYFIEKGITESLREGFELYPR
                     YNCSYDCVEFPSVKEVCDYIHQAGGKAVLAHPGETIKDRNMPFFKKKIERYLAFGLDG
                     IECYYPTHTEEITKLCLEICKEKNLLITAGSDCHGEFGKAQIGEMNVRLENLYLGELL
                     G"
     gene            complement(76740..77450)
                     /locus_tag="PLG49_00345"
     CDS             complement(76740..77450)
                     /locus_tag="PLG49_00345"
                     /inference="COORDINATES: protein motif:HMM:NF022674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HOQ15792.1"
                     /translation="MCFDMPELLELLIGLECINARINLDNNITLDLKNKELNNWDDEN
                     KDDKGWKLVSESCAWRIIKDSTIICGQYDEAEDIIPVLNELVASVIIDFKQTSPYDIS
                     LSLSNGCEIQFLAESLRDTVISIYSPNNRCFAFESGNIWTEMPANITEKELNREEELL
                     DKHSERCFKRWSKVVNQVPINRCSNCAYFLRLKGMFYFWDFGLCSNEASINDGRVVGI
                     CSGCDAFKEELEELEELE"
     gene            complement(77692..78156)
                     /locus_tag="PLG49_00350"
     CDS             complement(77692..78156)
                     /locus_tag="PLG49_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15793.1"
                     /translation="MDISQDKLLDILENEKDKEIIQKAVIYSALILEKCTCNVSTFEE
                     NDCINEKNYNKENLIEGLICLFHRKVIDPTVIIYALSKSYDISLKDFYLGVLKEAFEK
                     MDEAYNLLFQTIIALENIGEKIVASNDLSLSEVKENYLSVKKYLCQVVKEIN"
     gene            complement(78527..79081)
                     /locus_tag="PLG49_00355"
     CDS             complement(78527..79081)
                     /locus_tag="PLG49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOQ15794.1"
                     /translation="MVNNAMTADFLRSAYGGESMAHMRYLIWGEIARKEGFNNIGILF
                     EAIAHAEYVHANNHFNVLKAQKGDYTVPAGGVFGNTTTVENLQGAIDGELHEVEQMYP
                     VYLNTAEFQNEKDAKRSFHYALEAEKIHAKMFKEAQEAAKAGKDYDLEAVYICPVCGY
                     TVLDEAPEVCPICGAKKEVFKEFK"
     gene            79286..80392
                     /locus_tag="PLG49_00360"
     CDS             79286..80392
                     /locus_tag="PLG49_00360"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOQ15795.1"
                     /translation="MRLVPVSCLKGGEIIAKTVIDENGSRLLAKNTSFKKYYKKKLEA
                     RGIDRIYIADKLSEGIEIKDVISEKTRIKTINIVKDTLAIFKATQQIDVSQLETSVIQ
                     IVEDILSNKEILYDMVDIKTKDDYTYGHSVNVAVLCVMLGRKIGGDKSYLKKLALGGL
                     LHDFGKILVPDEILNKPDKLTEEEFDIIKQHPFQGYQIFKDNPLLTPISKVMILMHHE
                     KINGDGYPLGMTKNQIHEGARICSVCDVFDAMTSKRAYRNEYTIVNTLNEMERMTKNH
                     LDEDLFKTFRGMICYYPVGTTVLLSNETVAIVEQNKIGSIDKPTVRVIYDLKTKQEVF
                     RRVDLSATKEVKIERELISEEYINNPAILRAMRP"
     gene            complement(80426..80971)
                     /locus_tag="PLG49_00365"
     CDS             complement(80426..80971)
                     /locus_tag="PLG49_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15796.1"
                     /translation="MSKEYEANNENYKGIEESFRQQPMMNNKPYPSNMYPMGVYPNGM
                     MYYDDIDERDMEYMKRMYPELNQRIQKHIEYECDKMDYDGSPIYNEYPDQEIIEQMID
                     RVYERIIQEMPDLLDEGEMERQRIPRYRLVRSLAGALLLTELFGRRRRYRRRIPFYGY
                     PSYQYPYYQYPYQYSYPYPYY"
     gene            complement(81074..83521)
                     /locus_tag="PLG49_00370"
     CDS             complement(81074..83521)
                     /locus_tag="PLG49_00370"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ15797.1"
                     /translation="MKFFFDKEEFKEEVQETVKTLTKKTIDKASKQEIYQAVAFVLKD
                     LIADRWIETDNVYKKEDVKIVYYLSMEFLTGRALGNNLINLAVKKEVEEALAELGINY
                     NEIEEEEPDPGLGNGGLGRLAACFLDSLSTLGYPAYGCGIRYRHGIFEQKIIDGYQVE
                     YPDNWLEYGNPWEIFRPEYACEVKFGGNVRVEKKENGEYKFIQENYQSVIAVPYDIPI
                     LGYNNWTVNTLRLWDARAVNRFDLKKFDQGEYEKALEEQNLAQIIVKVLYPSDNHIKG
                     KELRLKQQYFFISASIQQAIKRFKANHTDFKELPDKVAFQMNDTHPSLAVAELMRILV
                     DEEELPWDEAWDITKKVCSYTNHTIMSEALEQWPIELFSRLLPRIYQIVEEINRRFCY
                     ELINQYKFDSEKIRRMAIIADGQVRMAYLAIVGSHSVNGVAALHTEILKHEVLKDFYE
                     IYPERFNSKTNGITQRRWLLKANPNLAQFITDTIGDGWITDLTELRKLLPYKEDEAFR
                     KRFMEIKYENKVKLAEYIKAHNNIEVDPNSIFDIQIKRLHEYKRQLLNILHVMYLYNQ
                     LKANPSMDFHPHTFIFGAKAASAYKMAKLIIKLINSVADVINNDASIDGKIKVVFIEN
                     YNVSAAEKLFPAADISQQISTAGKEASGTGNMKFMLNGAVTIGTLDGANIEIKEEVGD
                     DNIFIFGLTAEEVMNYYKNGGYNPWDIYNMNQDVRLVLTQLINGFLCPENTELFRDIY
                     ESLLNGGDPYFVLKDFESYAKTHKKMVEAYIDRERWVKMQIVNVALSGKFSSDRTIKE
                     YATEIWKLKSVKIRHDE"
     gene            complement(83572..84864)
                     /locus_tag="PLG49_00375"
     CDS             complement(83572..84864)
                     /locus_tag="PLG49_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOQ15798.1"
                     /translation="MDNTLAKELISFINDSPSPFHVVKQLEMALKDRGFTALDLREKW
                     ILEKNNKYYTIINDSAIFAFDIGEGNIEEEGFKIIGSHTDSPGFRIKPAPEIKSNRYM
                     KLNTESYGGPILYSWLDRPLSLAGRVLLKGDSPFKPEKILVDFKEPVAIIPSLAIHMN
                     RNVNQGIEFNKQKDTLPLLGMIKDELEENHKMIRFIADHLNIDTDKILDFDLFLYPVE
                     KASLAGIHKEFISAPKLDDLAMVHAGLKAMMNTKAKRGINIIFCFDNEEVGSSSKEGA
                     DSPTASYILERILLALGKSREDYFRALASSFMISADMAHAFHPNYEEKQDPTNRPLIN
                     GGPVIKLSANKEYASDGSTTAVFEQICKEAGVPVQYFVNRSDIKGGTTIGPIMARQLG
                     ISCIDVGNPILSMHSIRELGGVLDHKYIINAFEKYYSL"
     gene            complement(84982..88101)
                     /gene="ileS"
                     /locus_tag="PLG49_00380"
     CDS             complement(84982..88101)
                     /gene="ileS"
                     /locus_tag="PLG49_00380"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ15799.1"
                     /translation="MYNKVSTDLNFVEREKEILKFWKENNIFEKSISEREGCPTFTFY
                     DGPPTANGKPHIGHILTRVIKDIIPRYKTMKGYKVLRKAGWDTHGLPVELEVEKMLGI
                     SGKPEIENYGVEPFIKKCKESVWKYQSEWEKMSDRVGYWADMKNPYVTYHNEYIESVW
                     WSLKKIWDMGLLYKGHKIVPYCPRCGTSLSSHEVAQGYKDVKEASIYVKFAVKGEKQV
                     YLMAWTTTPWTLPSNVALVVNPNEKYIKAKCGDEVYILAEALAGTVLNEEYEVLDTMI
                     GQDLVGTEYEPLFDFAKPDKKAYYVVSDNFVTLTDGTGIVHAAPAFGEDDAKVGRIYD
                     LPFVQLVNEQGKFVDAVTPWKGVFVKDADPEIIKHLDGKNLLYKAIDYEHSYPFCWRC
                     DTPLLYYARDTWFIKMTEVRDRLINNNQKINWHPEHIRDGRFGNFLENVIDWGLSRER
                     YWGTPLPIWECSCGYRHVIGSIAELKEMGQNVPDDIELHKPYIDNVYLTCPECGGSMK
                     RVSEVIDCWYDSGSMPFAQWHYPFENKEIFEENFPANFISEAVDQTRGWFYTLHAIST
                     LLFDRPAYENVIVLGLVQDKDGLKMSKHKGNVVDPWTVLDKQGADAVRWYFYTTSAPW
                     LPSRFYGEAVSEAQRKFMGTLWNTYAFYVLYANIDKFNPKEYQLEYDKLSLMDKWILS
                     KLHSLIKTVDNHLDNYRIFEASRALQDFVDDLSNWYVRRSRERFWYKDMPQDKINAFM
                     TLYTVLVDLVKLIAPFTPFMAEEIYRNLVINVDKTAPESVHLCYYPQAEDKYIDKVLE
                     DNMDQVLNVVVLGRACRNTANIKNRQPLSKMYVKTGKELPELFKDIIAEELNIKEVIL
                     TDDVADFTSYSFKPQLKTVGPKYGKLVGKIREALANIDGNKAMDELRKGNPLSFEFDG
                     EKVELFEEDLLIETKQTEGFIAETERDLTVVLDTNLTEELIEEGFVREIISKIQTMRK
                     EAAFEVQDHIKVFYSGNEKLAQIIERNKEEIAAEVLGDHIAEDSSKEGYSKEWKINGE
                     PIELTIQRI"
     misc_binding    complement(88163..88387)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            88610..90073
                     /locus_tag="PLG49_00385"
     CDS             88610..90073
                     /locus_tag="PLG49_00385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOQ15800.1"
                     /translation="MSGLNLTLLTDLYQLTMMQGYREANVKNNVVVFDLFYRTNPSNS
                     GFAIAAGLQQVIEYIENLSFSNEDIDYLRTTNLFNEDFLQDLKNFRFTGDIYAVPEGT
                     VVFPKEPLLRIKAPIFEAQFIETALLNIVNHQSLIATKAARVCWAAEGDTVLEFGLRR
                     AQGPDAGIYGARAAIIGGCASTSNVLAGQLFNVPISGTHAHSWVMSFPDELTAFREYA
                     KVFPNTCILLVDTYDTLKSGVPNAIKVFKELKEKNIKPKLMGIRLDSGDLAYLSKKAR
                     EMLDQAGFPEAVISASSDLDEYLIADLKRQGAKINVWGVGTRLITSKDCPAFGGVYKL
                     SAEADKDGNFIPKIKLSENPDKVTNPGVKKIIRVYDKTTGKIKADLIALEDESFSEKE
                     PLRLFDPVNTWKQMKLKPGEFSLRELLVPVFINGKCVYQSPSVMEIRSYCQQELSTLW
                     DECRRLTNPHILPVDLSQKLYDLKKKMIDEIRQSNAD"
     gene            complement(90124..90552)
                     /locus_tag="PLG49_00390"
     CDS             complement(90124..90552)
                     /locus_tag="PLG49_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15801.1"
                     /translation="MKNRGFLFIIGLLSVLVLVSACTNNRTARNIRDDDGILDNGNDM
                     IDNDWGYDYNDDYGYDANDDLDLTPDGINAREENIRRGITTQVPTRTLPNPLNGTDMN
                     NRITNTRNNTNNTNVNNRNNVNTRTTTNTTRGNTMNQNNR"
     gene            90691..91410
                     /locus_tag="PLG49_00395"
     CDS             90691..91410
                     /locus_tag="PLG49_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ15802.1"
                     /translation="MRTLICILFILISGIISLLFSIKYAFLKKFSTQEKQIQYAYKSL
                     QRLTKGLLFCAGTRLNVKGKENIPKNQTLLFICNHKSLFDIPILVSSIDIPMAFVGKI
                     QLKKVPIISFWMKKINCIFMDRDDIRQSLKAINQGIEQLKNGQSLLIFPEGTRIKGEE
                     LGEFKKGSLKLANKSNVPIIPIYIKNADKILENQFPWIKATHIDVRIGKPIIIEKLDT
                     NSQKNLSEYVKKQIEILKADI"
     gene            complement(91453..92895)
                     /gene="cls"
                     /locus_tag="PLG49_00400"
     CDS             complement(91453..92895)
                     /gene="cls"
                     /locus_tag="PLG49_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOQ15803.1"
                     /translation="MEVVNWLLFLLLIVNILFAVLLVFFERRNPSTTWAWLMILFFVP
                     VLGFFLYLFLGQDLRKKKIFAAKEADDRLEKMIHQQEKILSRKDIELAYPSIKNSKDL
                     IYLHLVAHNALFTHDNTVEIFNDGNEKFDKMLEDIKKAKKFIHMEYYIIRNDWLGKQI
                     IELLTLKAKEGVEVRLLYDGMGCIRLSDKFFQPLLEAGGKISCFFPPFLPYINVRINY
                     RNHRKICVIDGQTAYVGGINIGKEYLGLSKKMGYWRDTHLRIKGGAVDYLELRFLLDW
                     RFSTKEPYLIKDKYFPPKEGLGYTGIQIVSSGPDSKWSSIRNGYLKMITLAKKRIYIH
                     TPYFIPDDSLLEALKLAALSGVDVRIIIPNKPDHMFVYWASLSYIGELLQAGVKCYTY
                     EKGFLHSKMILVDDVISTVGTANLDIRSFKMNFEVNAFIYDRIINNQLQEQFMKDLAD
                     SKEITREIYEKRSFGIKFKESISRLLSPIL"
     gene            93052..93435
                     /locus_tag="PLG49_00405"
     CDS             93052..93435
                     /locus_tag="PLG49_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15804.1"
                     /translation="MQVWQIILIILAVIVLILTVLYFVGKRMQTKMDQQQSLINQHKM
                     TTSILVIDKKKERIINSNLPKAVIDQFPKVFRYKKMPLVKAKIGPQITTLICDNKVFK
                     DLPTKKVVKVEIAGLYILGIKSVKK"
     gene            complement(93469..93780)
                     /locus_tag="PLG49_00410"
     CDS             complement(93469..93780)
                     /locus_tag="PLG49_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOQ15805.1"
                     /translation="MIKHIVMWRVKDETKYGSKEEALRQAKEKLEALKSKISEIVFLE
                     AGINYNTTEAAYDLILYSEFKTDEDLQTYQVHPEHLKVVEFMKEIVVPNSRVVVDYES
                     N"
     gene            93908..94801
                     /locus_tag="PLG49_00415"
     CDS             93908..94801
                     /locus_tag="PLG49_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOQ15806.1"
                     /translation="MKNWNIIAIIHEIYCRFQDDEVPALSAQLTFYLTLSFFPFLIFV
                     LTILNYTPISSEIFLSLLSNLLPKETYYLVYHTIKETVSTSNRTLLSFGMVTTLWSAS
                     NGVNSVIRGLNKAYDEEETRSFFRLRGISLIFTIVLSITILLSLALIVFGRYIGIFMV
                     THLGFNHSVSSVWNTFRQIIMITTIFVVLLFLYKYMPNRKLLYRDVLPGTIFSTFFWF
                     VLSNLFSKYIENFGKYNIMYGSIAGIIILLLWIYMSSIVLLLGGEINGVILFAKNGKV
                     KPRAKKFGFPIPFCKKKKNHS"
     gene            complement(94819..95547)
                     /locus_tag="PLG49_00420"
     CDS             complement(94819..95547)
                     /locus_tag="PLG49_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15807.1"
                     /translation="MKPEFKKYERRKNFIFKIVLALLVISLFYAVYMLFTAPAMEALD
                     KPYDRIKSDYVLMILQCLVGIIVMFLPSQIERRYRIEIPDLMEIIYFIFLFCAIYLGE
                     VRNFYFKIPYWDLILHGFSAAMLGAMGFFIVSYLNNMERPFVHLSPFFVALFAFCFAL
                     ACGTIWEIYEFAADCILGTNMQKFITEEGTVLTGQKALADTMEDLIVDAIGSLVVVTV
                     GYIYLKKKQIQSVRVEKAIKTEAD"
     gene            complement(95832..96653)
                     /locus_tag="PLG49_00425"
     CDS             complement(95832..96653)
                     /locus_tag="PLG49_00425"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOQ15808.1"
                     /translation="MSKNEGFLITNKNLYFSMKDPETDNLFKGKIDGKKIQELKVKQD
                     ADKMTVQINSVDLFVMDIKYHSDDLANFKGYIDRLTTGHLKIEDKEISKAIEKRLDKK
                     LYNRIKGFLYEDEYLLYVAWGLDSLSARDYVICTNQQLMIINREVLGISEQIKRFEYK
                     DIVSVSTAKSNNGGLITDMLSTAFKQCDLEIYVAGTSFRIETLLRLEAERIIKIVNEY
                     KRKGNKEEKVIIQEKVVQPKENVLDQIEKLAALKNSGILTEEEFQAKKTELLKRL"
     gene            complement(96845..97522)
                     /locus_tag="PLG49_00430"
     CDS             complement(96845..97522)
                     /locus_tag="PLG49_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15809.1"
                     /translation="MSLFDNFKKISDDVKKALDEANIMEKAKTATENIKKKAEDITGN
                     MSKTQRESFTMNRRKAEAIEILSHNLALENYFKDLQVNKDIYEIQGKFFKMTGTDGGR
                     GKIKITFFDEGETETKVVVDITVPTDTAGTMERNYKNIYNALLKSIDEKMIVKKEEFK
                     GLYKEVKEERQQKVEQIINKGIELVDKQTEKKRLEREEQNRVSQMIFSKKIKSIEENL
                     IDNKATL"
     gene            complement(97788..98267)
                     /gene="rlmH"
                     /locus_tag="PLG49_00435"
     CDS             complement(97788..98267)
                     /gene="rlmH"
                     /locus_tag="PLG49_00435"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOQ15810.1"
                     /translation="MKITIIAVGKLKEKYFHMGIAEYAKRLSRYCKLEIIELADEKAP
                     ENMSLAQEEEVKNKEGKRILDHIKEDTYVIALAIEGKVFSSEEMAKKIEDLSLKGRSH
                     ITFIIGGSIGLSKEVLNRADFQLSFSKMTFPHQLMRLILLEQVYRWFKIIKGEPYHK"
     gene            complement(98326..99582)
                     /locus_tag="PLG49_00440"
     CDS             complement(98326..99582)
                     /locus_tag="PLG49_00440"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ15811.1"
                     /translation="MEELIVNGGKRLEGEVFVSGAKNAAVAMIPAAILANDVCVIENL
                     PYIDDVISLADTLNEMGVKCDFLDPHTLRIDSRNLNNFCATYEAVKKIRASYYLLGAL
                     LGRYKQAEVAFPGGCNFGTRPIDQHIKGFTALGAEVVVEHGMIKARADKLVGTQIYLD
                     VVSVGATINIMLAAVLAEGTTTIENAAKEPHVVDTANFLNSMGANIKGAGTDVIRIVG
                     VKKLSGTNYMIIPDQIEAGTYMIAGAITRGDVTVHNLIPKHMESVSAKLLEMGVELEE
                     GDDYIRVKAKDRLKAVNIKTLPYPGFPTDLQPQMSALLSIAEGTSIITESVWENRFQY
                     IDELKRLGADIKVEGRVAVIKGVKKLSGAQVSATDLRAGAGLVLAALAAEGKTTINNL
                     QYIDRGYEAIEKKLNAMGAEIIRVKK"
     gene            complement(99778..100677)
                     /gene="yycI"
                     /locus_tag="PLG49_00445"
     CDS             complement(99778..100677)
                     /gene="yycI"
                     /locus_tag="PLG49_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HOQ15812.1"
                     /translation="MNWAKVKNILIVIFLILNIVLAYMNYQKNSSIYTLTSQQESNIK
                     KLLYENNIMLYTLLPKQYPPMRKLILAPVIFSEDEKDQIIKTFFNTNDGISIYIEKIE
                     PDIIQTVYKKDGKTIGFSDGRISYSEELNFKEQVIYSKEDAKKLADNFLKKMDYNLSD
                     LKVDFMEEEKGVYRFLYYETYKDSYIYNSYIEILIRAEGIEYADIYKMKPVKYTEFSR
                     SIYAPDEILFGFMNQFKKIEDNKSIIVIQKIDIGYLIESDKRTDSGWEAIPYYRITLA
                     DGRSFFLNAYTSKMSFENAKIQF"
     gene            complement(100709..102091)
                     /locus_tag="PLG49_00450"
     CDS             complement(100709..102091)
                     /locus_tag="PLG49_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15813.1"
                     /translation="MDKSRLKTLLLFALIIISCIQTGELWFGNLSSRNFFYNLWAKNT
                     SNIIKYSAEPISALQPARIIINLGFEGGVYNVLNRSSEEFKEAQEEMGDILVRALFEG
                     RFIKEESLDWRKIFSQKSILYAFTDSIPLSGFGNENSTISNIGEFNQLLVIPDKNINQ
                     EINCYFINEKSNKVYIVAIKQDQLNIYNLINKISEESTGLIYNSTKQIMMNQFENNVF
                     LPAFNEFPSYQLIRMKNSLKEAGKLNEEKLDKLVNPLFINPSLKRKQKISDGTIFYIE
                     GNVIVKYFPNGVLEYTSQTSSTVSTKTSFLESYKIAKDFLDEHEKAFSNNSSLETFLY
                     LSEQKETEKGWEFYFDYIINDMPYIFSREIAEETGMNHAVKIIVENKKVKQYKRLTWE
                     GYLLDKEVDLNVSFLEAINRFNFLVEEEAKIHDMYWAYYQESLDKDPEIFWIIHTEDM
                     IFPIKATQED"
     gene            complement(102115..102621)
                     /locus_tag="PLG49_00455"
     CDS             complement(102115..102621)
                     /locus_tag="PLG49_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15814.1"
                     /translation="MRKFLYIGIFALVMFFSTAEVRANSLENDPVSSVNIEKSGILDL
                     DMIKITAGITKKHEVTFDRYRNISGEADKGMVITFVVYQEGKKDNAKIYTQVVGASGL
                     FSQLIELKEGNNYIIIVAKQYEQEKAFKFRIDCKDTTIKKELEEIKIINILDTAVDGK
                     NALETLVK"
     gene            complement(102726..104486)
                     /locus_tag="PLG49_00460"
     CDS             complement(102726..104486)
                     /locus_tag="PLG49_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ15815.1"
                     /translation="MKSIQWRLVFIYLTLVLIVMISSGTFIIWQIRDNEYKKIHNELK
                     NTAESIRENIVSDDGSINPRWKDINTWVGDRSIFINKKIYILDDQGNIIIPDTEENGQ
                     EQWNKRVVYETIISKKPAVGDPVQILEGGLVQEYVDYAYPILVGDVLKNIIFIRASTQ
                     ETSNSIAIITNIIVIASFLALFITVIFGTYFAKTLTEPIKILTRKAKEMAEGHLDTKI
                     DVKSNDEIGQLTESFNHMAEELNQMLLTISGEKNKLETVITHMADGILAFDRKGHLIH
                     ANPAAHTMLDMTDKEQYFYDIFKRNGIELSFDEFLTMKPGYIKQHLMLVKDKYINGCF
                     ATYGRKKGESDGVIIVLQDVTEQKELEEMRKEFVANVSHELRTPLTTVKSYAETLLDG
                     AVDNKDLALSFLQVINNEADRMTALVQDLLELSRLDNKQVKFNLKSVNIAQLIIKSVE
                     QHQILAQKKNQNLGYSITNEKIIINIDPERIQQVLSNVISNAIKYSPEEAKINVSLSE
                     EEDFVVVKVTDTGFGIPESDLPRIFERFYRVDKARSRQMGGTGLGLAIAKEIMEHHKG
                     KIYAESEYGKGTTITMKFPK"
     gene            complement(104491..105189)
                     /gene="yycF"
                     /locus_tag="PLG49_00465"
     CDS             complement(104491..105189)
                     /gene="yycF"
                     /locus_tag="PLG49_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HOQ15816.1"
                     /translation="MAQKILVVDDEKSIADIIAYNLEKEGYEVLIAYDGEEALDLVFS
                     EDPDLILLDIMMPKIDGFQVCRKIREKKETPIVMLTARAEEVDKVLGLELGADDYVTK
                     PFSVRELMARVKANLRRIAVVEKEIKEEKNKNVIVHNNLKIDTEKYEVTKDGKIIELT
                     LREFELLKFLARQKGQVFSREVLLEKVWGYEYYGDIRTVDVTVRRLREKIEDDPSRAE
                     YILTKRGVGYYFKG"
     gene            complement(105342..106706)
                     /locus_tag="PLG49_00470"
     CDS             complement(105342..106706)
                     /locus_tag="PLG49_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ15817.1"
                     /translation="MINLERIQSIDMIKYFRLFVLIIVLTAVFYLGFEISQTPNAFSV
                     MLDGEVAGIILEEETADNLLNEVKLSIEKDLGGSIEVCSELFVEPVRASQKEIISKDE
                     LLTKLKNSLSYDIKAYVIKVDGVEQATLINNKEAEEVLEKIKAKYIEEGIKPELISFV
                     EEVKVEPKCIKVSDEMIAVNDAVELLTKSTEAQRIYTIEEGDTLWGISERYGLSIEDI
                     LKANPGLEEEGILQIGQEINLNIPKPILSVMTKEEFTYTEPIEKPVRYVEDDTQYKTY
                     IKVIEPGEEGEKEITAYKIKINGYEEGTEIISEKVIKEPKESVVVVGTKTPPPKSATG
                     SFRMPTSGTLTSTFGYRWGRFHAGIDLAAPIGTPVYAADGGTVVEAGWHGGYGYLVRI
                     NHGNGFESYYAHLSKIYVQVGQKVAKGEQIAAMGSTGNSTGSHLHFEIRLNGEAKNPY
                     NYLK"
     gene            complement(106862..109297)
                     /locus_tag="PLG49_00475"
     CDS             complement(106862..109297)
                     /locus_tag="PLG49_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOQ15818.1"
                     /translation="MKFGYFDDKNREYVITTPKTPYPWINYLGNKDFFGLISNTGGGY
                     CFYRDARLRRITRYRYNNIPIDNGGRYYYLFDNGDFWTPGWMPVKKDLDFYECRHGLG
                     YTSIAGERNDVRVNVTYFVPLGYNGEVHRVKVKNNSNADKQLSLFSFVEFCLWDAYDD
                     MTNFQRNLSTGEVEIEGSAIYHKTEYRERRNHYAFYWVNTEINGFDTDRDSFLGLYNN
                     LDAPQVVTQGKSNNSVASGWSPVGSHNINFTLKAGEEKVFVFVLGYVENDKDNKWIAL
                     NVINKEKAYAMQEQFKDNASVEKALEDLKVYWDGLLSKYILDSHDEKLNRMVNIWNPY
                     QCMITFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHQIPDRARERILDLAATQFEDG
                     SAYHQYQPLTKKGNHDIGGGFNDDPLWLILAVSAYIKETGDFAILDEEVPFDCNPDKT
                     GSLFDHLTASFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSTEPGESFQTYGDPNGRV
                     AESVFIAGMFVFIGKEYVELCKRRGLEEEAKKAQKHIDDMIKATIEYGYDGEWFLRAY
                     DAFGGKVGSKENKEGQIFIEPQGFCVMAGIGVEEGLAQKALDSVKERLETPYGIVLQQ
                     PAYTTYDYKLGEITSYPPGYKENAGIFCHNNPWIACAETVIGRGDRAFEIYKRIAPAY
                     LEEISEIHKLEPYVYAQMIAGKDAPNQGQAKNSWLTGTAAWNFVTISQWILGIKPDYD
                     GLKVDPCIPKAWDGYTVTREFRGTRYKIKIENPDHVSKGVKKVILDGKELDSNIIPPL
                     SDNKEHEVVVVMG"
     gene            complement(109654..111678)
                     /locus_tag="PLG49_00480"
     CDS             complement(109654..111678)
                     /locus_tag="PLG49_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOQ15819.1"
                     /translation="MKIISKAVISLSLAFCMSTSVFAQVSYIDVPENHWAYDVVQKAT
                     EMNWMTGNLQNEFKPQEVIDYFYFSGIAAKIAGYKDPLENINITEAEKKYADEAYQKY
                     KSVLDQYTKNFKKWDEAYYKNFNEEIAYLLQKGILKTEDLNKFIIKQADGTEQVASLS
                     REDMAVFIVRLIGKEEYAINNYTKTRFLDDALISENAKAHIAYLNDAGVLQGGENVKF
                     DPKGKVYKAVLAKVLVESVLGTGSQTSSNQTTSTDKEVYLQGKVEYFNSDYHYLGINS
                     GNKTSCYRIKENATYYIGTKETNKESIKKGDNVMIQLVNEGNIDYVVKITRIDTTSPV
                     SPDSSGNYTNTGSTSNHSYTDYQDNLNVSARKVEGIINSVKDSDVEIIVKYVNYKGEI
                     KEESETYNVNSNTIITKNNKELSLSDIKKGDIIVAQVKGSLLTKGEVIESQREVTGTL
                     IEKKRTNGLYKLVLEVKNDTVEYSIGSDVTIAKKGVDEAAWNDLRIGDKITLGLEYEK
                     VIDVWAEGYSSRVTGIIQEVLVSANQSKVTIEHEDGTIKTYPLMYGAKIVDRLTDEEI
                     SIYDIRLGQEAVLYLDSMEVEELVLSKQIKLSTVQGYIENISFSGDYIDIRTESDFIG
                     TKRIRMGKGVEIFKDNRRLSRRDLKKGMLVVITMKTYGGDQAEKITILSK"
     gene            112155..115265
                     /locus_tag="PLG49_00485"
     CDS             112155..115265
                     /locus_tag="PLG49_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ15820.1"
                     /translation="MKNLKKKLLSLALAAAVVLSSTNFAFAGNLSNADKLNILTNLGV
                     ITGEGNGVIGTQTMTRYRAFVMQLKLMGKYDEMNNFAWEGKATFKDINSSHSQFIRKL
                     AAYLKAHPEIGISGDHLGNLNPMAPISGREYMKIMLVRLGYVENVDFTWASIPTFAYS
                     KGLIDNPTEVEGSNIQLLQIADFTYDALMSKPVGSEKTLGEQLGLFNFDITLDKVDAT
                     TENETIIISGSITNDGTVTVNGKAASVNNKKFSAEVALTLGENQITITAVDSNGIKVE
                     KTIKVVREYKDLKVLKVEGYNLKQFAIEFSKELDKDSVTNSRLGLGANDIFEISKDGK
                     TILVTLDTPLRQTTDTKYTIKDIKDITGKKIAKTEIVVNVFDNELPEVEKVIAKGNQY
                     ITVKFSEPVENADLLSSYRIDESLVRGSIEANSKKTEFTINLKTALADGEHTLSISND
                     IKDAAGFNLKAVDIDFTVVKDEDAPEAEIISASQTKVVVEFNEEIKGLDKSQVSWKSG
                     SKKGVASSVVQSDNDEFVYEITFAEGNYVPLNGATLILENVKDFSDNIAKKIEINFVP
                     EVDLERPEVIDVEADENNQNILYVTFSEDVNTNGKYVLIDKNNKEKTASSIAYADSDA
                     KNKIKVTFTGVAAGDYTLEVSEVTDLSPLANELLPVLLEITINDLERVSIDSWGRIVS
                     GDSKRILIRFTEEVDEKKATNLNNYKFRFVSGALYSLPDDTEINLLGDDKTVEIIFPE
                     EWKLNGRTYDLDDFESIKYIQVEKVTDVAGNEMYAQLLDIDAGTLTLDSEAPVVVSAA
                     VTGENTIELELNNPIDEKSLDRRDFIVKLVSGLSLNVYRVDYSDSDDEYKLTLYVREK
                     LDQNAKFDGTGLRLTLAETVSTKNIYGSALSMASALAIEDKYNPEATVASAVYGNKTE
                     IVFEVGEAVQFGFGSGLALVTSESVEDGVTTVTLERGSAANDLLISRFTVKKDGKKLN
                     IEKIQIVDGSKIVFVINDGNEDWDGDKLEITYNGLDNPAYSITDASGNILVNFDDVEV
                     SIENIN"
     gene            complement(115344..116939)
                     /locus_tag="PLG49_00490"
     CDS             complement(115344..116939)
                     /locus_tag="PLG49_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOQ15821.1"
                     /translation="MRRHKNWLITGLIITFLMPSSLRADTSGVSLLWNGERYLLKNQP
                     IVKSGTSLIGMRELFEILGAEVEWDANKKIIKAQKDENYIELKVGTKDAYKNGEKIEL
                     SLAPEIKDGVTFVSLRWIAEALDAQVEWNKDEAAIYITTKENINPAEEDPSGEKLSDE
                     KTRDESVPMDNNLVENNIPKLKIEAIEYKNARELSLEDVKRMAVGGNISLRLQNLAAE
                     RAEEAAVIASRTPMVSDGLGGYVAIEEDAVVSLLRRSTADMGAEAAKKQKELIKESVQ
                     FMAEKAYYEIVELEEQLAITQASVELSRENLDIIRKRYELGLESRYNLSQAEMEQEQL
                     EKQLETLKLSLDSKYTELNNLIGQPTNMRYILNKNIGYKPLEDISLKGHISDMISKDF
                     YVWYSKKAKELKDLERKSHSTTWTPNGESYSMKDNEYKQAELSEEATIKNLEKSLESR
                     YNQILQLEKQIEAQKLNIEKAKESYRILIQQYKLGMIIPLKLKQGELAIHNAELELQK
                     LMAQHNQLKTAFYNPALMPDYAN"
     gene            complement(117095..>119486)
                     /locus_tag="PLG49_00495"
     CDS             complement(117095..>119486)
                     /locus_tag="PLG49_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ15822.1"
                     /translation="IGEVGGSYLTIEEKEYLMEEIEPGAYFNLILKNGIWENVSSEEP
                     TLYNIYGQKVTNQVYGQKVTNQVYQIVAFDDFDGFAMKKLTDTKVQIQVYRKVYGKTS
                     APGNFQFAIPLDNVKCTEEADVELTIDDYNSGVSKGTYVIAVFAKDATKAKIDSKSFN
                     NVLDLNTLEIDELKIGTLKDGELRLEILNDDFAWIWNPGVAREVTVTGSGTLSNAKMK
                     AVIDVDKSSNSVLVLDIKGINAVGRRNQRATLYISGLQIIAKRNADFGEVEVEIDGSH
                     DDGCGVTKEVLKVGEYVDFGIKVSVAEEKDLVAGYDKQTYKITLEEVIQGSWASERRT
                     TIKFPDYVRIADKSAVKIIAADCENIAERRTNYSYVDDDKNVVKSPYAYVECDDHWID
                     FANIKKEDPTKTTKLVFEVTLEAKAASSGDITATFEGNALLGYEEELVLGEVTVPFTV
                     DVEAKELKAGLKVQDAGTITVTETDKGKLTKGTKLYFELEDYVSWETKPEVKVDGDIE
                     VEASILEEKAVDKYFETVPIGVYTYEVYEYDVNTKDYTKFRATASTWEEVLGICGAAD
                     GTIDKDKYYVRRRTKTSTLSRIYEYEFAKTSGRVLVVEVKKASRKQASTITLEGAKLL
                     VDRTIPEGTYDMAVRTLGDKWNGWGSWYKDLYVDLTTPNTEDIEQSKNAKYTATFTLD
                     SVNYTVDGVTLQADVAPYAKDGRIMVPVRYVAQGLGVNPDDVLYSNKTVTIMNGNRIV
                     QLVLGSNVMKVNGAEITMDTVAEAKDGRIFVPMGWIAKGLGVPVQWDNTTKTATFN"
     assembly_gap    119487..119586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(120066..120383)
                     /gene="trxA"
                     /locus_tag="PLG49_00500"
     CDS             complement(120066..120383)
                     /gene="trxA"
                     /locus_tag="PLG49_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ15823.1"
                     /translation="MAKELNAQEFQTEVLEASELVLVDFFATWCGPCKMMAPIVDQVS
                     EEMKGKAKVYKIDVDQARDLAAKYRIMSVPTFIFFKNGEVVEKIPNAVSKETLIEKIN
                     ELA"
     gene            complement(120557..121237)
                     /locus_tag="PLG49_00505"
     CDS             complement(120557..121237)
                     /locus_tag="PLG49_00505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ15824.1"
                     /translation="MRKVIRFLSFLTLLSLILTGCNSGEEAASPQFKLPEKGEEIAVL
                     TTDMGVIKIRFFPEYAPKAVENFKTHAKNGYYDGLTFHRIINDFMIQGGDPNGDGTGG
                     ESIWGQPFEDEFSTEVLHYRGAVAMANSGPNTNGSQFFIVQKPVVEEELLQQMKLAGY
                     PEEIIHLYRKKGGTPWLDFNHTVFGQVFEGMEVVDKIAAVEMADPNSGIPKNPVIIKK
                     IEIITYEE"
     gene            complement(121328..122878)
                     /locus_tag="PLG49_00510"
     CDS             complement(121328..122878)
                     /locus_tag="PLG49_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOQ15825.1"
                     /translation="MKPLKPEKEIEKLLQYALDKGLITEHDMIPTRNALMDLLEIKEP
                     YKGAIDKEDLKEDVYDILDKLLDYAFEKGILKENSITCRDLLDTKIMGILTPRAGEVI
                     KKFERLEREQGIEKATADFYAFSKATTYIRMNRILKNLYWTVHTEYGELEITINLSKP
                     EKDPKEIAAAKNAAQENYPKCLLCLENVGYPGRINHPARQNHRVIPLKLSDEAWYFQY
                     SPYVYYNEHCIVFNREHVPMQLTRKTFERMFDFIERFPHYFIGSNADLPIVGGSILSH
                     DHFQGGRYIFPIEKAKVLYGFKHPDYKNVSCEILSWPMSVIRLKAADRKALEELAFFI
                     LESWRSYFDKEADIIAFTLEKDKKIAHNTITPILRKRRKGQWELDLVLRNNRTSPQYP
                     DGIFHPHPDVHHIKKENIGLIEVMGLAILPGRLNRELEAIEKILTGEISVHKQMEKKP
                     ELVKHKAWIEEMQFKYGTVKEGRNILQKEVGMKFKKVLEDAGVYKQTPEGIKSFVEFL
                     EYMSFEKI"
     gene            complement(122892..123881)
                     /gene="galE"
                     /locus_tag="PLG49_00515"
     CDS             complement(122892..123881)
                     /gene="galE"
                     /locus_tag="PLG49_00515"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ15826.1"
                     /translation="MSVLVCGGAGYIGSHAVEELIATGEEVVVVDNLQKGHKKAVNPK
                     AKLYIGDLRDLNFMDRVFKENPIHAVMDFAAESLVGESVTNPLDYYDNNVYGMICLLK
                     KMKEYQVKNIVFSSTAATYGEPENIPLMEDDKTFPTNPYGESKLAVEKLLKWCDQAYG
                     IKYVVLRYFNAAGAHANGRIGEDHRPETHLIPLILQVALGKRKEIMIFGDDYNTHDGT
                     CIRDYIHVTDLAHAHILALEKLKKDGPSAIYNLGNGQGFSVKETIEAAKKVTKKDIPV
                     KIVGRRAGDPAVLVASSDKIKKELGWEAQYKSLEEIIETAWNWHLSHPNGYAD"
     gene            complement(123883..125052)
                     /locus_tag="PLG49_00520"
     CDS             complement(123883..125052)
                     /locus_tag="PLG49_00520"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOQ15827.1"
                     /translation="MNTKQLMSKFTEIFGEIGKPDVFFAPGRINLIGEHIDYNGGRVL
                     PCALDFGTTALARKRKDSIIKLASMNYPLQVTLDLNRITFENKDAWGNYPKGVIDQFL
                     RRGFTLGGFEALYYGTIPTGAGLSSSASIELVTAMILKSFFDCDISIIDMIKLCQDAE
                     NQFVGVNCGIMDQFAIGMGKKNMAIYLDCDSLNYEYVPIPLKGIKLVIGNTNKKRTLA
                     DSKYNERRRECERALAALQNRLDIKSLCNLTPGQWEENKDIILDPVIRKRAEHVIYEN
                     ARVKEAVEKLSQGDIMTFGKLLNQSHESLRDLYEVTGFELDSMVEEAWKQEGVLGSRM
                     TGAGFGGCTISLVKEEAIEEFINRVGENYKKKTGLQGDFYIAGIGDGVRKIRLEG"
     gene            complement(125096..126124)
                     /locus_tag="PLG49_00525"
     CDS             complement(125096..126124)
                     /locus_tag="PLG49_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ15828.1"
                     /translation="MAATISDVARLAGVSITTVSRVINNNYPVKEETRRKVERAIKEL
                     DYKPNLLARSLINKKTKSLGVVVPSITNLFFPVVVKGIQNISDREGFSLFLCDTDSRG
                     EKEKKQIQNLIEKQVDGILVIDPLMDNIENDFYEKIALNIPLVIVNGYHKGIRANFVL
                     SDQEMGMIQAMEYLIGLGHKDIAFLRGKDSYSYDLKEALYYELLKKHKIAVKEENILK
                     IKDGNGLETVALTIEIVEERLEKANGPTAIIACNDWMGIGAVQAAKKLGKSVPKDLSV
                     IGYDNILISELSEPRLTTVDQNMYALGETAAKLLIEIIEDTATNFKKIIMDTHLIIRD
                     SCAAPAKI"
     gene            complement(126254..127330)
                     /locus_tag="PLG49_00530"
     CDS             complement(126254..127330)
                     /locus_tag="PLG49_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOQ15829.1"
                     /translation="MHLKRIILTGGGTAGHVTPNLALIPKLQKEGWDIRYIGSKTGIE
                     KELIEEKMIPYYGISSGKLRRYKDIKNLTDPFRVIAGLFEAIQLINKLKPSVIFSKGG
                     YVTVPVVMAGWLKRVPVIIHESDITPGLANKLAFPFAKVICANFPETLSYIPKSKGIL
                     TGTPLREELYRGDRETGRKICNFKNDKPVIMMMGGSQGSIILNTVLRNILPVLLEPFN
                     VIHICGKNNIKDSLNDIAGYKQFEYVDKELPHLFAAADLLISRAGANSIAEIVSLKKP
                     NILIPLSARASRGDQILNARSFYKQGFSQVLEEESLTEESLFNSIMEVYKNREKYIES
                     MKNSGISNGIEKIMEIINVCAKEG"
     gene            127711..129534
                     /gene="asnB"
                     /locus_tag="PLG49_00535"
     CDS             127711..129534
                     /gene="asnB"
                     /locus_tag="PLG49_00535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOQ15830.1"
                     /translation="MSGFCGFIGNTVDKEQILKDMTNAIIHRGPDSDGEYIDDTIAIA
                     FRCLSISNPQDDAQPLFNEDEQLVLVSNSRIYNYESLKELLEEKGHLFKSQSDAEVLI
                     HAYEEFGTKMLDHLRGMFSFVIWDKKEKRLFGARDIFGIKPFYYYKDNDYFIFASEIK
                     SILKHRSVHKELNPDALENYLSFQYSALEETFFKNIYRLPPAHFFIYNNNEMHLQRYW
                     EPVFETENKSLEEYVDEIDKTIQESIELHKMGSAEVGSLLSSGVDSSYLASCFGGDKT
                     FTVGFDYDQYNEIDYAKNLTKEIGINNYHRLITTKEYWDILPKVQYYMDEPLADPAAI
                     ALYFASELAREHVKVVFSGEGADEFFGGYNIYKEPLDIKVLTSLPMPIRRFLGKLASI
                     LPFDIKGKNFFIRGSKRVEERFIGNAFIFTEKERKELLKHKSNAASPFSLTKAFYNKV
                     KDKDDITKMQYIDIHMWLPGDILLKADRMTMAHSLEVRAPLLDKEVFNLAAKLPTKYK
                     VNKKSTKYAFRLAAKRHIPENVANKKKLGFPVPTRVWLREEKYYSIVKEAFSSPIAEK
                     FFNTDKLLLLLNEHYSGKKDNSRKIWTVYIFLIWYQQYFEA"
     gene            complement(129613..130032)
                     /locus_tag="PLG49_00540"
     CDS             complement(129613..130032)
                     /locus_tag="PLG49_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOQ15831.1"
                     /translation="MNSQECLFCKIAKGEIPSATIFENSEFKVILDKFPANKGHILII
                     PKEHMENIYEMDSELGGKLFSLAINIAKILKKELNIEGLNILQNNGKIAGQSVFHFHL
                     HLIPRYQDDEVNITWKPLKPTNEELTDLANKLYKSLQ"
BASE COUNT        40468 a        25942 c        18003 g        45611 t
ORIGIN      
        1 ttatggatta ttatattgta tattatcaaa aaagtccatt ttaccaaacc attatattgc
       61 ttattaaaaa aataaatcct cattccatat catcattttt aagactcgat tggtttataa
      121 ataatagttt attttattta caatttaatt ctgatatgtt atgcttaata aataaaatta
      181 ataactttag cacaatttat aagggtggat aataaatgga tattaatttt gaactgtata
      241 aaattttcta ttacgttgct gaaaacctca gcttttctaa agcagctgct aaactttaca
      301 tttctcaatc tgccgtaagc caggcgatta agaccttaga agacaattta aatatcaaat
      361 tatttttcag aaacacaaaa caagtcaaat taaccaacga tggggaaatt ttattccgcc
      421 atattgaaca agcttataat cttatcaaaa acggcgaaag aatgattgat gaaatcaact
      481 ctcttgaacg aggcgaaatt cgtatcgggg ccagtgacac catatgtaaa tattatcttc
      541 ttaactattt caaagaattt cataaactct atccgaaagt aaaaatcctt gtaaccaacg
      601 gcacttctcc taaatgtgtt gagctcttaa aacagggcag tgttgatttt attatatcta
      661 atctgcctaa ccagtatgcc gattcttcca tgaaaatcaa aaaaataaaa gctatccaag
      721 atgtttttgt agcaggaaac cagtttaaag aattgaaaaa cagggaaata tatttaaagg
      781 atttagaaaa ctatcctttt ttgatgctgg aaaaacaaac tgccacaaga gatttttttg
      841 acaatatgtt agtggaatat ggcacccata taatccctga aattgaatta ggcagcatag
      901 atcttttagt agagatggca aaaatcggat tgggtattag ctttgtctgg gaaaaatgta
      961 ttgaagaaca gcttaaatcc gatgaaatat ttattgtcaa tgtaaaagaa aaaatacctg
     1021 aacgttactt aggtattatt acccataaac acattcctct ttctattggc gctaaaaaat
     1081 ttattgaact gctccattaa tgaccataaa aagaagcctg tagaataaga ttgacttcat
     1141 caattttttc ctatatactt tgattatcaa aatatttgta aatcaaataa aaggagtggt
     1201 gtaatgaagc ttccttcatt aaaaataggt catcttgttg ccgatattcc tatcatccaa
     1261 ggtggtatgg gaattggagt atcaagggcc aatttggctt cagccgtagc taacgaaggc
     1321 ggaataggta taatttctgg cgttcagatt ggttttgaag aaccggattt tgaaacaaat
     1381 aatcatgaag caaatatccg aggcctcaaa aaagaaataa aaaaagcaag agaattaagt
     1441 cctaaaggta ttataggcat caatatttta acggctgtca accattataa agaactggtt
     1501 ttaacagccg taaaagaaaa aattgatctt atcatatgtg gtgcaggtct gccagtagat
     1561 ttacctgaac tggttaaaaa ttctgcttcg aaaattattc ctatagtatc ttctgccaaa
     1621 gctgcatccg tcatcattaa aatctgggac aaaaaatatc agtatcttcc tgacgccctt
     1681 attgttgaag gaccaaaagc aggaggacat ttaggttttc ccttggaaat tctaaatact
     1741 catcccttac ccagcttaag caatatcctg aaagaagttt tatccgtcat taagcctttt
     1801 gaagaaaaat acaacaaaaa aatccctgtt attgcagcag gaggtatttt caatggaaaa
     1861 gatattgcaa aatatcttaa agagggagcc tcaggcgtac aaatggcaac ccgttttgtt
     1921 gcaactcatg agtgcgatgc ccatattaat tttaaaaaag catatcttga tgccgtaaaa
     1981 gaaagtatcc aaatcataaa aagtcctgtt ggtatgcccg gtcgcgccct tagaaatgaa
     2041 tttacacagt ccttagaaaa aaacaaagaa aaaatccaca aatgttataa ttgcttaaaa
     2101 ccttgtaatc ctgccaccac tccttattgt atatcaaaag cattaattca tgctgtcagg
     2161 ggtgatacta aaaaaggatt aatttttgcc ggaacgactg cttacaaatt gaataatatc
     2221 atctccgtaa aagaattaat aagagagttg gttcaggaag ctgaacttac tttaacctaa
     2281 taaataattt aaaaaaaatc tactaactaa tttatattag ttagtagatt tttcttacag
     2341 ctcgtcctta tctaattctt taagatattc gaccagtaaa tcatccaata cttgacttac
     2401 tagcatgaca tctttacttg aaatgttttc ctctcccaaa ataatcaagt catttaggtg
     2461 ttcccttgtt tgctcaattc ttgttttcag cacttcgagc ctactatacg acatttaaat
     2521 tccccacttt aaaatacttt aatatacctt aagtcaattt caataatttt tcaatgagca
     2581 tcaagtatgt agtttttgat aatataaata aaaactagaa aaattgatta agtcttctat
     2641 tttataataa ccttaattta attatcatgc aatagcaaat aattactaat actataatac
     2701 tattacttta ttcctatatc ttttctgtaa tgcatattct caaaccggat cttttcaacc
     2761 ccctgataag ctgtgcggat tgcttcatcc agtgtctttg ctattcctgt aacaccaagc
     2821 acccttcccc cattggttac tatctgatca tcttttttag ctgttcccgc atgaaataca
     2881 attatattat tttcgtgtaa attgtcaagt ccaaagattg gaaatcctgt atcataagaa
     2941 agaggatagc caccagaagc taataccaca cacactgccg cattatcttc ccattcaacc
     3001 tttatctcat gaagcctttt atccacacaa gcttcaagta tttccaaaag atctcctttt
     3061 agtcttggga gcacaacttg agcttcagga tctccaaatc ttgcattata ttctaaaact
     3121 ttaacgccat tttctgtaac cataagacca aaaaacaata ttcctgtaaa aggtcttcct
     3181 tcttttttca tagcttctaa agtaggctta aagatttttt ccatacaata attctgaatc
     3241 tcttgtgtat aaatccggct tggagaaaag gttcccatac caccagtgtt taagccctgg
     3301 tcattatcta aagctctttt atggtcctgg gcacttacca taggtacaat ggtatcacca
     3361 tcacaaaaag acaatactga aacctcttgt ccaactaaaa attcttcaat aacgacttta
     3421 tttcctgcat caccaaattt tttattttcc atgatatcga taacggcttc tttagcttct
     3481 tcataattat tgcagattaa aacacccttg cccaaggcaa gtccatctgc ttttacaact
     3541 atggggaaag attgcgactt aagatattct aaagcatcct tcgaatcgtc aaatacttca
     3601 taatttgctg tggggatatt atattttttc attagattct ttgaaaatga tttactgcct
     3661 tcaataattg ctgcattctt tgttggacca aaaattttaa gtcctctttc ctgaaaaaca
     3721 tccacaatac caagcattaa ggggtcatcc atcccaacaa cggtaaaatc aattgcctta
     3781 tcttgtgcga aatctgctaa agcttctata tctgttgcct ttatattaac acactcagcc
     3841 aattgagcta ttcctgcatt accaggtgca caaaatattt tatcaacttt tttactttga
     3901 gataatttcc agacaattgc atgttctctt ccaccactgc caacgactaa tactttcatt
     3961 gctttacctc catttactta atcttcaagt atttttactt ttttatcttt aatcataatt
     4021 ttattatcac aaaacaattg tatggcttta ggatatattt cccactcagc ttcctccatt
     4081 actcttcttt gaagactttc aggtgtatct tcttctttaa cttgaaccgc tttttgtaaa
     4141 ataatgggtc ctgtatctgt tccttcatct acaaaatgaa ccgtcgcacc tgttattttg
     4201 acacccctgt ccaatactgc ctgatgaact ttaagtccat aataaccagc tccacaaaaa
     4261 gaagggatta aagaggggtg aatgttcata attctattct caaagcgttt aacaaacttt
     4321 tcatccaaaa ccagcaaata gcctgccaaa acaaccaggt ctacttgtct ttcttcaaaa
     4381 tgtttgatta agcagtcggt aaatacctct ttgctgtcaa aatctttagg tgaaatacaa
     4441 attccttcaa tttgatgttt ttttgccctt tctaatgcat atgcatcggg cttgttactt
     4501 acaacggtta caatttttcc attctttatt tttccatttt caattgcatc aatgatggct
     4561 tgcagattgg ttccccctcc ggaaaccaaa acacctattt tgtacatatc tccacccctg
     4621 tttcactttc aaccacttct ccgataacat aagccatttc tcccatacct gaaagcaagg
     4681 aaagggcttt ttctttttcg gtttcactga taataacaac cattcctata cccatattaa
     4741 aagtattata catactcatc ctgctgacat tacccagttc ttctgctaat ttgaaaatag
     4801 gaggaatatc ccagctgcct tcataaatct ttcctccaag tccttcaggg aaaatcctgg
     4861 gaatattttc aataaaaccg ccaccagtaa tatgactaat gccctttatt tcaacttctt
     4921 cttttaattt ctgaatggtt ttgacataaa ttttagtggg ttttaaaagt tcttctccta
     4981 aagtacagcc caaaagttct acatattcat tgatattgtt tttattagga ttaaaaatct
     5041 ttcttataag agaatagcca ttgctgtgaa taccagatga agccaaacca attaataaat
     5101 cgcctttttt tattttgctt ccgtcaataa ttttttcttt atcaacaata cctacacaaa
     5161 agcctgctaa atcatattca tcttcaggat aaaaacctgg catttctgcc gtttctcctc
     5221 cgattaaagc tgccccggcc tgtttacaac cttctgcaac acccttaaca atctcagcaa
     5281 ttttctttgg tatatttttt ccacacgcaa tataatctaa gaaaaacaaa ggctctgctc
     5341 ctgaacatac gacatcattg gcgcacatgg caacacaatc aatgccaatg gtatcatgtt
     5401 tgtcagcaag aaaagcaatt tttaactttg taccaacacc atctgtgcct gaaaccaata
     5461 caggttcttt cattttaata ttggcaatag aaaataggcc accaaaacca ccaatatccg
     5521 ttaatacctc aggtctaaaa gtagccttga cgtgttctct cattaaaagc acagcttcat
     5581 aacctgcttc aacatccaca ccagcacttt tataatccat actctttttc ctccttatca
     5641 acgttaattg gataatttcc atcaaaacaa gcagtacaaa aaccgcattt tgctccaata
     5701 ggtgttttct taagaccttc tatcgataaa aaagcaagac tgtcggcacc gattaattca
     5761 gcaatttctt caatggaata atgattggca attaaatttt ctttatgagg tgtatctatt
     5821 ccaaaataac atgggtaata tacaggaggt gagcttactc tgatatgaac ttcttttgct
     5881 cctacctctt ttaacatttt gacaatcctc ttgcttgtag ttcctcttac tatagaatca
     5941 tctatcataa taattctctt accatcaacc tgacttttta aagggtttaa ttttaatctg
     6001 accgcttgtt ccctcatttc ctgacttggt cgtatgaaag ttcttccaat atagcgattt
     6061 ttcattaagc catccccata aggaatacca ctttccatag cataacccag ggctgcagta
     6121 agaccagaat ctggtacacc gataactaaa tctgcatcta cggggtgctc tctggcaaga
     6181 atttttccgg cttctaaacg tgcttcatat acatcagccc catcaataat gctatcaggt
     6241 ctggcaaaat atacaaactc aaaaatacac attttggatt ccttaggtgc ttctgtttgt
     6301 atagaagtta taccctcttc gttaaccact acaatttctc ctggctttac atctcttaca
     6361 aaatttgccc ctatggtttc cagggctgca gtttctgaag aaaatacata ggaatcatct
     6421 ttcttgccaa tacataaagg cctcatccct aaaggatctc ttgcagcaat taatttatga
     6481 ggcgtcataa ctaataaaga atatgcccct ttgatttgct tcatcacttc aaccaatgat
     6541 tcttctatac tatgatgtct tattctctcc tttgaaagaa gagcagcaat aacttcagcg
     6601 tcgctataag attgaaataa aaatcctttc tcctctaaat catttcttat tttagctgca
     6661 ttcaccagac ttccattatg agcaagagcc atattgccct tagtatattt aagaactaaa
     6721 ggctgagtat tctctttttg gttttctcca gacatagccg atcttacatg tccaatagcc
     6781 atttgaccat gtaaatgatt aataatcacc tcgtcaaata cttctgatac cattcccatt
     6841 tctttgtgat aaagaatggt tccgttatca ttaatagcaa tccctgcact ctcttgtccc
     6901 cgatgctgta aagcatataa accaaagtaa gtcatttcag cggtattcca gttatttttg
     6961 ttataaatac caaaaacgcc gcattcatca tgaaacttat catctaataa ttcccgttgc
     7021 atagtaaagc tcctttcgaa aaaatataac cattttaata aaatataacc attttaatag
     7081 gagatatatt tattatgcct gaattatcca tgatgtctat tttttgggat gcaattaaaa
     7141 acagattcca tatgcatgaa atctgtccaa atatataata cctttatttc agtcgaacat
     7201 tctttcagac actttcaccc atagtcagga aatttacggt tccctggtag aaacgctcaa
     7261 accatattat tgagcatata tgagtattaa tttgatatct atatatgtac cataggcaag
     7321 atgctactgt caatacaatt atcgaatttt atctttaata aataaaaaaa cgttcggttt
     7381 tattttttca ttttcaataa ataggattct ccacctattt tttctagttc ctgggctttt
     7441 tcttcaacca tatctttaag gctttcctta taagcttcta ctttcttacc aacttctaaa
     7501 ttaaaggagc caataatttg ggcagctaat attcctgcat ttttagcgcc attgattgca
     7561 accgtcgcaa caggaattcc cggcggcatt tgcacgattg aataaagaga gtctatccca
     7621 cttaaagtag aagttttaac tggaacacca atcactggaa cagaagtaat cgaagcaacc
     7681 atgccaggaa gatgcgctgc tcctcctgca ccagcaataa taacctttaa gcctttttct
     7741 tttgcattct ttgcatattc atataatctt tgaggagtcc tgtgtgcaga aacaatggta
     7801 agctcatatt caataccaaa ttcatctaat actctggctg cttcaatcat taccggaagg
     7861 tcagagtcac tacccataat tattccaact tcaaccatac cacttgtcct ctctacttat
     7921 aatttactgg ataaattttt ataaactctg ctgcagtgtc cactttttgt attgcctctt
     7981 ccaaagtttt ggcaacagca gttatatgtc ccattttcct tcttggactg gttttttctt
     8041 tcccgtaaat atgaagaaaa acgcccggaa tacttaaagc ttctttaacg ccacagattt
     8101 ccggctcacc agcacccagt tcttctccaa gtatatttcg cataactgtt ggctgaataa
     8161 gagaagtatc tcctaaagga atacctgtaa tggcacggat atgctgttca aattgagaag
     8221 tcacacagcc ttcaatagaa taatgacctg aattatgagg ccttggcgca acttcattta
     8281 ccaatagttt tttatcttcc gtgacaaaca tttcaacaca aaacatgcct acaccttcaa
     8341 aaactttcat caccctattg gcaagattca tggcccggct ggttaaagtc tttgaaattt
     8401 gcgcaggtac catggtcttt atcaggatat tattttgatg tatattttca ccgacaggat
     8461 aaacagccgt ttcaccatct attcccctgc aagccaatac agagatttcc tttgtaaaag
     8521 gaacaaattc ctcagccatt aattcttctg tttccccacc aagactttca aaagcaaaaa
     8581 gaagttcctc tgggctgtta actaccatgt tgccttttcc gtcatagcct ccccggcaac
     8641 tttttaatat aaaaggatat ccaaaaagtt taccagcttc tttcatatcc tccacagagg
     8701 atacataaat aaaatctgga accggtattc tctttttctt taataattct ttttgcttgt
     8761 atttattttg aataattgca aggcttttgg gcgtcggata aataatatgg ccttcttctt
     8821 ctaattcttt taatacttca acacctatat gctcaaattc ataagtgata acatctaccc
     8881 tgtcagccag ttttcttata gcaacctcat catcaaaaga tgcgacaata tgctcatctg
     8941 ctaaactgtg ggcaggacaa tgaaaggttg ggtcaagaat aactgcgtaa aaacccattt
     9001 tttttgcttc cagcaacatc atctttccta actgtccgcc accaataatt ccaatcgttt
     9061 gatttaatat tccatatgtc atgatctcac ctctaccatc aatcaaattt agcaacttta
     9121 aatcttcggt tattcaaaca gatgcttaaa ggcttcaatc gaataattca gccgttttgt
     9181 accagcagaa attcctacaa ttttaccctt cgtatcgtat tcaagacctt caaaagcttt
     9241 aatatcatct gctttaatcc cataaacatg gttttcttct tctccatttt ccagtacagt
     9301 cgaaacaagc ttgtccctgt tattttcaag aacagatgat aattcaggaa ttgtatccaa
     9361 aggtagaaaa gctcctatct tcatttgctc tttataataa ttttcctcaa aacatactat
     9421 atctaattct cctactgcag aatatgttgc cagcttttga atacttatat tagtcgcttc
     9481 attgggttct tctgtattga ttatataaaa atcaactctg acagaccttc ccagatcttc
     9541 ttcttttata attttttctg taaatatttt ttcccaattt gcttttttct ctaaatcaat
     9601 taagtcccca ttctgtaatg aaaaagtcat cagaggaata tttttgtctg cctgaaaaat
     9661 acttagtatg atattgatcg caaagactaa aatcaccaca ccaaaaataa aatgccactt
     9721 ataataataa ataacatgtt ctattttttc ttttaaattc atttctctaa aattccccac
     9781 actccatcac tccatagcct gattattctg tccttaaact ctccaaattt tatgcgcctt
     9841 actattataa caaatatttg ataaataatt aatattatat tttataaata atttatagga
     9901 ttctaaaaaa ttattgtgtc attttaacga tatgcttttt caacaacttc attatttttt
     9961 gctaataacc tgattttctt ttatgattaa aatgtgtttt tataaaatat atctttaatt
    10021 tttaagaaaa tcacatacct tttctggcat gtgatttttt taatcggatg taaatgataa
    10081 agggcaaata agctatcaaa aaagcaacta acgcaaccaa ttccattgaa agaatatacc
    10141 atggccaggg acctaaaaaa tccattatac taacaacttg tggctttctg cagataaaaa
    10201 ggtaatttcc tcctgtgatc aaattaactg cagctacaaa aagcatgtac aaattactta
    10261 ttagcatagt cttgaatata gaagaaacct taggtctgta ttcattgaca aaagtcataa
    10321 atattatagc aataacaata agtccgtgac caataaaaaa atgataaaat aaaaaatgtg
    10381 tagcgttgta accggataac tctggtgtaa tgagcgccgc caaaacaccg cacatggccc
    10441 aaaaatacaa aaattcatat aaaaaataag atttagtgaa taataagatg atggtgccaa
    10501 taagagccat atcacataaa tgaaggggta attcccttga aaaagaccat aagtgattta
    10561 cataaaccca gatataataa agaacttcta tagtaatcaa taaaaaagca agaccatgcc
    10621 tgaaacggcg attaattttt tcttccttta atcgatgatg aaataaataa atcatcaggc
    10681 agcaagcaaa aatgattact ataacagtaa tatgctcagc agaaaatagc tcaaaagctt
    10741 tccccttgta attttcccca aaaaagtccc tcatcataat actctactcc taaagcaaag
    10801 cttttaaact attatatgca aatcaagttt tttatagaca tcttgtcttg ttgtgtttct
    10861 tctaaaaaag attgccttat aggcaatcct tttttaatct ttactttatt ttccgacttc
    10921 ctggttttac taaaggcagt tttccttctt tgcataaagg acattcttct gcttcatagg
    10981 aaacgacatc tactgttaaa gctgctctta actttgtacc aaaatcaatg ttaccattgc
    11041 ttctgtcaac taagaccgct acacctgcta attcgccgcc ttgctcttta acaatgtcga
    11101 ttacttcctt aacagaacca cccgttgtaa tgacatcttc tgcaaccaga accctggcac
    11161 cttcaggaat agtaaaacct cttcttaagg tcattttccc gtcttctctt tctgcaaata
    11221 aatttttaca gtttaattgc cttgccaatt cataggctat gatgatacct cccatagcag
    11281 gaccaacaac aatatcaatc tggtcatctt taaattcttc cgctaaacct tttgcaattt
    11341 cttccgcgta attgggatat tgtaagattt tcgcacactg catatattga ttgctatgtc
    11401 tgcctgaagt taacaaaaaa tgtccttcaa gcaaaacttc tgtttcttta aagatttcaa
    11461 tcacacgctc tttgtttaac atcttcaatc ctccattatc attgtattta tttttcttac
    11521 tgatactaaa tcatacctct tatttcatcc aaggactgga taccataacg aaccatatat
    11581 tctttaatgc cttcaaggac atctatcgtt gctcttggat tcctaaaatt agctgtccct
    11641 acagcgactg ctgttgctcc ggctaaaatg aattctatag catcttctcc tgttgatata
    11701 ccgcccattc caatgatggg aaccgatact gccctggcaa cttgataaac cattcgaagg
    11761 gcaacgggtt ttacagctgg tcctgaaaaa ccacctactg tatttgcaag gattggtttt
    11821 cttttatgaa tatcaatggc cattccaagt aaagtattaa tcagggatat accatcggca
    11881 cccccatcaa caactgccct tgctatttct gtaatatccg ttacattagg gcttaattta
    11941 acaatcaggg gttgtttggc ataacgttta acttcttttg taaccttatg agccatttta
    12001 acatctgtcc cgaaggttac gccgccttct ttaacattgg gacaagaaat attcagttca
    12061 attaaatcaa tgtctgcatg ggacagtctt tctacaactt cgcaatattc tgatacagac
    12121 tttcctgcaa cattaacaat aatttttgta tcgtattgac gtaaaaaagg aatatcgttt
    12181 ttaataaatt cttccacccc tgggttttgt aaacctacac tgtttaacat tcccccgtag
    12241 gtttctgcaa ttcttggagc aggattgcct ttccagggtg tactggatac gccttttaca
    12301 gtaacagctc ccagttgatt gagatctaca aattcaccat attccctgcc agaaccaaaa
    12361 gtcccggatg ctgtggtaac aggattcttt aactcaacac ctgcaatatt cactttaaga
    12421 ttatgttttt ctttaatcat cccataccac ctcatcactc caaaagactg gtccatcctt
    12481 gcagactttt ttatgctgcc agtctagttc atctttttct ttaattttac atacacagcc
    12541 tacacaagcc cctatgccac atgccattct ttcttctaag gatacttgca tagctatctc
    12601 attttctttt gcccaagctg caatggcttt taacatgggc ttaggaccac agctatatat
    12661 aatctctcct ttagcattat tctttttaag aagttcaaga acattcccct taaaaccttc
    12721 tgaaccatcc tctgtcgcta cgtgaacatt ggctccataa actttaaatg cttcaagtaa
    12781 tatagattga cttctgaagc ccaaataaac ttctttttcg ccttttaact gctttaccaa
    12841 ttctaccaga ggaggtgttc ctatgccacc ccctacaatg atgtttttag ctttactttc
    12901 atcaatttta aaaccatttc ccaaagggcc aaggacttca attttttctc ccgctttcag
    12961 ggtactgaat tcctttgtcc cttggccaac cactccataa accagagtaa ttctacctct
    13021 atctttatca atttcacaaa tacttatggg ccttggtaaa agcttagcct cccctttaca
    13081 atataagttt acaaactggc ctgctttgac ttccctggca ataccgtctg cctccagggt
    13141 cattttgtat atattgtctg ctatcaattg attatctagt ataagtgcat gtttaatttc
    13201 tttcatgact tcctcccagg ttatatacct caatatcatt aaaactgatc tttgaatcca
    13261 tcaccttaag taaagcttta actgtatcta aagaagtaaa taaatctaca gaggcttcaa
    13321 tggctgctct tcttatcctg aaaccgtctc ttctggaatc attgcctttg gtcggtgtat
    13381 taatgaccat atcaatcata ccgcttctta taatatctaa tatattagga acaccttcac
    13441 taatcttttt tacctcggat actggaatat tggctttttc cagtagcttt gctgttcctt
    13501 ctgttgctat aaagcggtag ccgtagtctg caaaagcctt agctataggc aaaaattctt
    13561 cctggtcata aggcttaatg gtagcaagaa ttgtgccatt tcctttagga attttgatac
    13621 ctgctgctaa aaagcctttg tacagagctt cttcgagtgt tttaccaacc cccaggactt
    13681 ctcctgtaga acgcatttca ggtcctaaac ttacctctac cataggcagc ttttctgttg
    13741 aaaaaacagg aactttaacg gcaattaatt ctgcttcttt atacaggtct gtaccataac
    13801 ctatatcttt taaactttct cccagcatca ctcttgttgc aatatcaata acaggtaccc
    13861 ctgtcacctt actaatataa ggaactgtac ggcttgaacg gggattaact tcgataatat
    13921 acaattcatt ttcatattct ataaattgaa tgtttaccat accgataaca tttaaagcaa
    13981 gagaaatcct ttttgtataa tctaaaattt tttctttaat ttccttagaa atgttttgac
    14041 ttggataaat ggtaatactg tctcctgaat gtatcccagc cctttctaaa tgttccataa
    14101 tgccagggat taaaatgtct ttaccatcac atatcgcatc cacttcaatt tctcttccaa
    14161 gcaaatatct gtcaatgagg acaggatttt tagaatctct ttcaaaagcc tctcccaaat
    14221 actgctttaa ttcttcttca gaataggtaa tttccatgcc ttgtccgccc aaaacatatg
    14281 aaggacgaac cagtaccgga taacctaatt ccccggcttc ctccagacct tcttcaagag
    14341 tccagactgc cttaccttta ggccgtttaa tcttgagctc ttctaaaagc gcatcaaact
    14401 tttccctgtc ttctgccata tcgatttgtt ctggttttgt ccctaaaatg ggtatattca
    14461 tctctctaaa gaatttagct aatttgatgg ctgtttgccc cccaaactgg agaattactc
    14521 cgtcaggctt ttctttttcc acaatattta aaacatcttc ttctgttaaa ggttcaaaat
    14581 acaacttgtc tgatatatta aaatccgtac tgacggtttc cggattattg ttaacaataa
    14641 tggtttcaat gcccgctttc ttcaaggaca aaatgctgtg aacagaacaa tagtcaaatt
    14701 ctattccttg tccaattcta atgggaccag aacctataac catcactttt ttcttattgg
    14761 aaacttctac ttcatcatat tcatcataag tagaatagta ataaggcgat tcagcatcaa
    14821 attcccctgc acaagtatct accatcttat agacaggacg gatatcccat ttttctctta
    14881 actgataaat aagttgtgga ctaaccccca taagatcagc aataccttta tcagaaaaac
    14941 cttttttctt tagcagataa agatattctt ttgttaaatc ttctggctgc atttgcttta
    15001 aattttcttc ctgctctaca atccacttaa ttttttccac aaagaaagga tccattcctg
    15061 taattttaca cactttttcc acccggtagc ttcttcttaa catttctgcc agatcaaaaa
    15121 ttctttcatc atcaggaacc tgcacccttt tctttaactc gtccaaactt cttcctgaag
    15181 aagatttctt atttaatgaa tactgaccta tttctaaaga acgaataccc tttaaaagag
    15241 ctgcttcgaa gttatttcct atagccatga cttccccagt tgccatcatt ttagttccca
    15301 gtgaacgttt tgctccatgg aatttgtcaa aaggccattt tggtattttt ataaccacat
    15361 agtctatagt aggttcagaa caagcataag tcttgcctgt tacactattt ttaatctcat
    15421 ctaaccgata tcctaaagcg atctttgttg ctacttttgc aatgggataa cccgtagctt
    15481 ttgaagccag agcagaagaa cgactcactc ttggattaat ctctattacg gcatattcaa
    15541 aactttcggg attaagagca aattgtacat tacagcctcc tttaatccca actgcatcaa
    15601 taatatccaa agcagcgttg cttaacattt tattttcttt ctccgataag gtctgacatg
    15661 gggcaaccac aatactgtcc cctgtatgta cccctacagg atcgatgttt tccatattac
    15721 atacgacaat acgattaccg tcttcatccc gaatcacttc gtattctatt tctttccaac
    15781 ccttaatact tctttcaaga agtacctggc ctacacgact taaatgtaat ccatgggcca
    15841 gtatttcttt tagttcttcc acattattgg caattccgcc tcctgtccct cctaaggtat
    15901 aagcaggacg tacaatcaga ggatagccaa ttttttcagc aaattttatt ccatcttcta
    15961 cattagtgac aatggcactt tctacaacag gctggcctat tttttccatt aattttttaa
    16021 agccttctct gtcttctcct tctttaatgg actctatgga agtcccaata attcttacac
    16081 catatttatc aagaatacct ttatcataaa gttctacagc catatttaag cctgtctgac
    16141 cgcccatacc tgccagcaga ctgtctggtc tttctttttc aataatccgt tcaatcgctt
    16201 caatggtgat gggttcgata taaatactat ctgccatttc cttatccgtc ataatggttg
    16261 ccggattgct attgataagc acaacttcta cgccttcttc ccttaaagcc tgacaagcct
    16321 gagttccaga gtaatcaaat tctgccgcct gacctattac aatagggcca gaacctataa
    16381 ccattacctt ttttatactc aaatcccttg gcatactgat tctccctcca ttgctctgat
    16441 aaagcagcta taaatattgc caatatctct gttcccggga tagaattgaa ccccaaaaag
    16501 aggtaaatct ttatgcacaa aaccttctat cgtatcatca ttgatattga catgagtctt
    16561 tatcgtatct cctggcaatt cttctatacc ataagaatga ttttgagttg ttataagaat
    16621 tttgtttgtt cctaaatctt taaccggatg atttgcacct ctgtgtccat gctttttaac
    16681 tgtgccccca agcgccagag ctaaaagttg acaacccaaa tccaaagcta atatgggttt
    16741 tttaccaata agctttttaa tattttctac tgcccaagaa atatcacaag gatttcccgg
    16801 accattagat aacaaaattc catgaggatt actttctaat atctcttccg gagttgttcc
    16861 cgcaggaaaa actgtcatcg cacaatttct ttcctttaaa cattcgataa tactttgttt
    16921 aactccaaaa tcgattatag caatatggtg ccctttagct tctatgacat atttttcttt
    16981 tgtagtcact tctaaaacgg cattaatatt gtgatagctc cttatttttt gttcaatttg
    17041 actagctgtt aaatctctta ctgtaataat acctctcatg gttccttttt tacttaaaat
    17101 tttcgtcaaa gctctggtat ctataccttc aattccaatc actttatttt gttttaaata
    17161 tccgtccaat tccatttcac aacgccagtt gttcggaaaa tctgcttttt ctcttacaat
    17221 aagccccttc accttaaccg tttcagattc tatatcttct aaattgatac cataattacc
    17281 gatgagagga taagtcatgg caacaatttg tccataataa gagggatctg tcaataactc
    17341 ctgataacct gtcatttgcg tattaaatac aacttcccca accgcttctt tcatataacc
    17401 aaaggcattt ccattaaagg ttgtaccatc ttctaaaatc aattttgctc tcatgcgaat
    17461 gccccctgca aatctttttt catatcaata actgcttgtc ttgaagcaag cgcaaattct
    17521 ttttcactat aatctttttt gtatttatcc aatttataag cagcaataat tcctctggat
    17581 gaatttacaa tagcccccaa gccattctta tcaaaacaaa ccgccaggtc tttagctgta
    17641 gccccctggg caccataacc cggcaccagg aaaaaagtat gaggcataag ttccctaagt
    17701 ctttttgcct gttcaggatg agttgctcca acgacagcac ctacctgact ataacctttt
    17761 ttccctatta actccttacc ccagtcagaa accaacttgc ccactctttc gtaaagcttt
    17821 tctcccccta agtctaaatc ctgaatctct ccactgttgg gattagaggt tttaaccagg
    17881 ataaataagc ccttatcata atccttacag tgcttaatat aaggttctat ggaatcataa
    17941 cctaaataag gattaagggt aatgctatct tcatgataaa cttcaaaggc ttcatcttct
    18001 actttaactt ttccaatatg cccatcggca taagcttctg ccgtggaagc aatatcgctt
    18061 ctttttatgt ctccaatgac aataagtcct ttttctttag cataaagaat agtatcaata
    18121 taagcttgaa taccttccgg tccaaattgt tcatacatcg ctacctgagg tttaactgcc
    18181 ggtactaaat catatacagc atcaatgatg gctttattat attttaagaa agcccaggcg
    18241 gctgcctttg gagtcttacc ccagctttca taagcttctt cttttataaa agcgggtaca
    18301 aaatctaatc ttggatccaa ccctacaact gtaggatttt ctttcatttc tatctgatgg
    18361 attagtctgt ctatcatcct tatcctcccc ttctaatcaa caatttcccc attatctaaa
    18421 acaatctttc cagctacaat agtatattta acccttcctt taaccttata accgtgaaaa
    18481 ggagagtttt tactctttga ttcaaaatgg tttacatcaa tcacgtattc ctcattaggg
    18541 tcaatgagag tgatgtctgc aatttttcct gtttctaaag aacctttatt gattcccaaa
    18601 agctttgcag gatttgtact cattttttct atcaattcca gagggcttaa taccccttct
    18661 tcaaccagat gcgtaattcc taaagccaca gctgtttcta aacctacaat gccaaaggct
    18721 gccttatcat attcacaatt tttttcatcg atatgatggg gtgcatgatc tgtagcaata
    18781 atttctataa tgttttcttt taaggcttgt ttaatgcttt ctacatcctt tttactcctt
    18841 aaaggcggat tcattttggt attggcatca tagccttcta ccgcttcatc tgtaagagaa
    18901 aaatgatgtg gacaaacttc agcagttacc ttttcacccc gcatcttagc ttctttaatc
    18961 aactgtacac tacctgctgt actaatatgg caaatatgaa gtttcacccc tgtactacgg
    19021 cctaagatga tgtctctggc tacaattacc tcttcagaat ctgcactgat gcctttcatc
    19081 cctaatcgct gagccatttc tcctgcattc attaccccgt ctccagcaag acttttatct
    19141 tcacaatgag acaagacagg aatattatac atacttgcat atttcattgc tttctttaat
    19201 agacctgcat tctccaccga tttgccgtcc tcactaatgg cacatatccc tgcttctacc
    19261 attttcccaa tattagaaag ctcttctcct ttttgtcctt tggtaatact gccaatagga
    19321 agaacattca caagcgcttc tctttctgct ttcatcttta tatattctac aagaatttca
    19381 ttgtcaatca ccggattggt atttggcata cagcatatgg tagtaaaacc tccttttgct
    19441 gctgccctgc tcccggtttt tatcgtttct ttatattcat aaccaggctc tcttagatgt
    19501 acatggacat caataagacc tggtgtaacc catagacctt ctgcatcgat gattctgtct
    19561 gccgaatctt ctatcttatc tgcaagagct atgattttcc cgtcctcaac caatatatct
    19621 tttttcccat ccgtcctgga caagggatcc agaagccggc cattttgtat cagtaacttc
    19681 atctttcttt ctcctttcca ttaatagatc tattatagcc attctaaccg caacgccatt
    19741 tttcacctgt tcatgaatca aagactgttc cccgtcaata agctcagttg ttaattctac
    19801 cccccggttc actggaccag gatgtaaaag aatagcatct tctttggcat attgcattaa
    19861 ctctttattg acaccaaagg tttgactata ttctctaagg tcagggaaaa ggccaccttt
    19921 ttgtctttca agctgaatac gcagccccat caccacatct gcatctttca gtgcttcttt
    19981 tatatccgtt gttacctttg ccccaagaca ttcaatattt ttatccacta aggtcgccgg
    20041 accacataca accacttcgg cacctaattt cttcaggcca aagatattgc ttcttgctac
    20101 ccggctgtga acgatatctc caataattgc tacttttaaa tcctgaaagt tctttttctt
    20161 ttcatatatc gtaaaaaggt ctaataaggc ttgtgtcgga tgttcattga ttccatcacc
    20221 ggcattaata acagaggcct taacatttct tgctaacaga ttagccgtcc ctgacatact
    20281 atgacggata accattatat caaaacccat ttgatccagt gtgattcccg tatctattaa
    20341 actttcaccc ttattaacgc tgctggttgc aacgcccagg tcagtcacct ttgctccaag
    20401 atacttacca gccatggaaa atgacatttt tgttctcgta ctgttttcat aaaaaagagt
    20461 aacgatatta taattttcaa ggatattttc tcttaatttt ttattatcta tcttcaactt
    20521 cattttttct gccgtatgga taatagacaa aatttcaaaa ggacttaaat acctgattcc
    20581 taacaagtcc ttattttcca acatttaatt tcctcctttt ttgatttcat taaaaaaagc
    20641 aataaagaga ataattattc tctttattgc ttttaattgg taataataaa aatggaaata
    20701 taaaagcagg tagaaaatct aaaacaaaag ctggttctac ccccatattt ccacaagtta
    20761 caacttgatt atattgattt tttacataat aaaaaaacgc accgatgtct cgcatcttca
    20821 ctcttccttt ccggcctcac aggaccaaat taaaggttaa tatgcgttcg agtaagttac
    20881 ctatctgttg ccttatttat tatcttaata atcatatcac ggtcaatata ctttgtcaac
    20941 gaagtttaag aaattagtat atgtttttat ttttagactt tttagatttc tattttttaa
    21001 taaagaatgt aattcataac ttaatattgt attaaagtaa ttccatcggc ttgacaaacc
    21061 agatcaaagc tactacatat attttataat gcatgttttg ctttattaat ttttttattg
    21121 aattattcca ccttaaaaag aatatattct ttaataaatc taagtcatct taaaataata
    21181 aataaaatat atcttagcta atcttaagct tataaatcat atttttcgtc gtaaatcttt
    21241 tcaatttgaa aattagcttt taaggctaag tcctttaaaa aaccctctgc atcttcaaat
    21301 aataaacaaa aattatacca gccttcatgg tcataggtct tatatattct gttataatcg
    21361 cttaatgtct tattaaatgt atacttgcaa taaatatgta tataaaaatc tgccggagta
    21421 taaacaattc ttttaatatc tgaataataa atggtatcga tgccataagc atctgtggtc
    21481 caatcccctg caccctcata atactcatat aaaactgtca ttttatcatc tgccagtacg
    21541 agtttaatct tattttttct tgcaaaatta ctcctgaaag tatctgaaaa atatctcttc
    21601 cctgaatctt ttcctaaaat tttattgata atgcttttaa ggattgccaa caagaactct
    21661 aacagagcca ttaacaaaac aatgataaaa caaactatcg gatacattaa taaaaactct
    21721 ataatttgaa taatcatctt gtcactattg ataaagccaa aacccttata attaaagatg
    21781 aaaagtgtca ccccaaaggc cactacaaaa aacaaaatga ttgatataaa gtctttaatc
    21841 ttttcttttt tactgacttt attttttctt atttttttca ttagtttttc attataataa
    21901 taaatttttg tcacggaaaa atcactccaa aataataaat taattatttt tattcaatcc
    21961 attcgtccaa taaacccttt attaatttaa agaatacttt tcatcttcaa taatttcaca
    22021 gatccttcct gtcttctgag caaaattgtc aataaaatac atattattaa tataaactag
    22081 cggaagatga taccatccgc gtttattgct ggaaaagtta tcgatttcct cttcataatt
    22141 gcaataaaca tgtaatacct cgtcaaccgt agaccttaca agcctttcaa catcagaata
    22201 cttaatttca actttaattt tactgtttga ctcagtattc tctaagcccg gactattata
    22261 tctgtcatat tggtacaata aactgctatc ggtaaaaaac gtcttaatat tttccctgtt
    22321 atcaatatta cagtttacat accgataagt tcttctatcc tttttgacca gcttttttct
    22381 ataaacccat tgaacaatat atttaatgat aaaaccaaaa ataagtgctc taataaaaac
    22441 tgtgccaaaa accagtaaaa aaaataaagg aatatacaca tctgtatcta ttacatctgt
    22501 atctattata agatcaaaac cttcaatata aaaaaataat accagcccca tcccaataat
    22561 caaagtgata atcaagggga aaagaacagc taatatagta agttttttct taatattatt
    22621 atatctttct tttaaaaagc tgtctctgat taaattttta ttcatactaa tttcctcaaa
    22681 catatttacc cccactcttt ttttgatttt tcttctttat tttatcataa gttatctttt
    22741 tatacaaatc aaattgacta aaatttaaaa tataagtaaa aatccaatac aaaatgcttg
    22801 tattggattt ttaaaaatca ctttcatcta tgatgaaatc taaaaccgtc tgaagtctct
    22861 tacgggcttc ttcaattttt actttatctc tgctgttaag ggcactgtca aattttaaaa
    22921 gctcatattc caatcttttt cttttctctc caatactttc ttcataaagt ctttcagctt
    22981 ttaacagcaa gagtttattt gcttcatgtt ctcttggatg tattttaaga tatgataatt
    23041 cctgcatgcg tttctcaatt tcctcatccg tcatatctac atcctgaccc ttgatgatta
    23101 ttctttctgt ctttcctgta gatacaactt taacttcaac ttcaagtatg gaattaatat
    23161 cataggtata agtgacgtcc accgcttctt ctccggctct tcccttagga acttctacag
    23221 ttaattctcc tattaaaaca ttatttgaag caaaacggct ttctccctga agaattttaa
    23281 cccttatttt tgtctgatta tcataaaccg tgtataatct ttctgtcctg ctggcaggaa
    23341 ttacagtgtt acgttcaatg ataggacaat aatagccaga ttcaaaccga ttttcttcaa
    23401 tttctcttac gacttccgtt cccagtgtaa aaggacatac atcggttaaa atcacttctt
    23461 taaccgcctg attcctttct ttcatagctc cctggatggc tgcccctaaa gcaacagcct
    23521 catcaggatt aatactggta ttaggaatca tcttaaaaag tttactaacc attttatgta
    23581 taataggtag tctggttgca ccaccaatca taaccacttg atcaatatcg gaaagcttta
    23641 tattggcatc agataaactt cttttaattg gctgtttaat acgttccaaa agaatctcac
    23701 aatttttttc gtagctgtca atgtcaatct tcatttccat cagttcatta ttgatattgc
    23761 atgacataac cgaactagat tcctgagcaa attttttctt acattcttcg gcttgctttc
    23821 taatatggga taaggtttta atatcaagct cttcagcttt taaattatgt tctttcataa
    23881 acatatctat caatacctgg gtaaaatctt ctcccccaag aaaattgtct cctgcgactg
    23941 catgaacttc taatatattg ccaaagagct ctaaaataga tacatcaaag gtacctccac
    24001 ctaaatcaaa aactaaaaat ttcatatctt tattgctttg gtaaagacca taagcaatag
    24061 cagctgcagt aggctcactg ataatacgtt ctacctttag acctgccaat tctccggctc
    24121 tttttgttgc ttttcttctg gcatcattaa aataagccgg aacactgata atggcttctg
    24181 tgaccttttc acccaaatat acttctgcat cttctttaag agagcgaaga acaaaagaag
    24241 acaattcttc aggtaaaaaa gtcctgtgac ctaaagtata tttctttttt gaccccatat
    24301 ctcttttgaa aacactggca caagtatccg gatatagtat catgcgttcc tttgctgtct
    24361 cacccacata aacctggtca ttttcatcaa tacttacaac tgatggagta agctttttcc
    24421 ctaaacggtt gggaattatt ttaggtccat cttctgtaaa ataactcaca agactattgg
    24481 ttgtacctaa atctattcct ataatcataa ctgcttctct cctatttttt ctttcatttc
    24541 atttacttta ccaatatatt ttatgtcatt ttcatcaagt tttaaagcta tttgatattt
    24601 ttccaaagca gaatggtaat cctgttctgc ctctaaaaac aagccttcta taaaatataa
    24661 tttagcacat ttgagaaaag aacatctttg gcagcttaaa cattttttca tctgttccac
    24721 gtattcttta gctttttcat attcgccaag ggacaaatgc caggaagcca tataatatag
    24781 tctttctttg cgttcattgg gattatttaa ccaactgtct aaactattga attttctgtt
    24841 tatgtagtca agagtttttt caaaatattt tttggccaat ttttcctttc ctaatatttt
    24901 taaaacatat aaaatacact ccgtcaattc ttcaaaataa acattatcat aattataata
    24961 ctcaaaatac atatatgctt ttttataata ttttaatgcc tttttataat ctctaagcag
    25021 ccaaaaaata tggtctccca tatatctata tggctcagaa cctataacat cttgaaaaat
    25081 acctttttta taaaacttta tagcattctt attatcacct ttacaggaat acaaataacc
    25141 cacatccaac cagtattgat tgacatagtt tttatcaatg tctaacttcc ttttataata
    25201 agctaaagcc atctcccagt tttttaaacg cacataaaca taagcaatat attcaagaaa
    25261 atcactattc tccgtttttt cataaatctc ttcataaagt tcaattgtct tctcaaattc
    25321 cccaacaacc atataataag tagataaatt tctatgagtg attagttcat tcgttgtaca
    25381 gtacttaagg gccaattgat aatacttgta tgcttcatca aagttgccaa gaaccttata
    25441 ggaccagcct atattattat aaggatacat gtcctctttg tcatattcta ctgccttctt
    25501 aaaagcctca atagcttttt ccatctcata accgtctgaa tagattaaac ctaattcatt
    25561 atagtaatag ccattaggtt gaatttctac ctggcgcttt gcatgtttta cagctaattc
    25621 ataatgttcc cttttaaaat acttgttgta ccatcttcta tagatatcct tgattttatg
    25681 attaacataa gaatgctctg gctctaattc aacagtcttt aaataatatt cgagggcttt
    25741 ttgatcattg tttagttctt cataacaata tcctgtaaca taatacccat aatagtagtt
    25801 aggacaatca ttaattaatt ttttaaagac atttatagct tctttatact ttttaagata
    25861 ataaagaata tctcctttta attcaatact actcacatcg tcacttactt ttaatctctc
    25921 attaattgct ttaagagcca aatcaaattc gtccatttcg taataacaaa acgataattc
    25981 atataataag acagaagaat cttctatgtc attaactatt tcttgtccat ttttttcttc
    26041 ctgctctgtt ttttcttttt gaactttatc atatagtttt tttatctcat cagcaatttc
    26101 tcttttttct tccttagtgg ttgcttttat tcttttaatc cgtagttcct gataagcaag
    26161 ttcattactt ttaattccag cttctttagc cttatctaaa acccttttag cttcttcata
    26221 tctttcaaaa ataattaaaa cttttactgc caatatgtat attttaggat aaccatcata
    26281 aatttctaca gccttatagt aatcatcaag aacttccctg cccattttta agttataaaa
    26341 agcctcttgt ctatacacat atgctgggaa gtaaccacta tctttagata ttatcctgtc
    26401 acaaacatct acacataatt catttttacc caattctaaa tacaattggg ccttttcaca
    26461 taaaaattta agcaaatctc cttcatttgt ttctgtttca atagctaaat cataatactc
    26521 tatacattta tcaatatagt tttcgtctgt ttttgattta ctaaaatgat aataacattt
    26581 agcaataaag taataagcta aagacaatct tctaatgatt tttttgtttt catccgaaat
    26641 gtcagcatca tctttatctt taattttttc ttttatttta agtatctctt ccagccaaat
    26701 cttagcataa ggataggcct gttcataatt ttccatgacc atataattgc gggtcattaa
    26761 attataatac tcataataaa tcttatcatc taattccata tcctttatta aattcagaca
    26821 ttcttcaaat aaattatttt ggaacaaact ccaacctaat tctaatttta gctttttatt
    26881 ttctggctct ttttcgattt gctctttata aatttcaatc aaatgttcat tcactttttg
    26941 caaatcttct attaagcttt ggtcataact ccactcttca agtaactcta acaatttctc
    27001 ttttgattgt ttatagtctc cttttttata aaaatattta taaactgctt tcctggcccc
    27061 ataatgcctg ggtgattctt ttataatctc ttctgcttct ttgtaggcct tttcgatatc
    27121 tccggactcc atggtcagta acatcaaata atattttata aatagattat catattttct
    27181 ttccatgagt ttttgtccta tttccttagc tgtttcaagt tcattggaat tcatatagta
    27241 tatcattttt tctacgtcca aatatggatg ccagagattc gtcttttcta ttgtatctaa
    27301 aactttatgg aattccccat attcttttgt atcattcatt ctccttagag cataataaca
    27361 attaataaac tcatctacat ctccatcttc ttttccttca aagagtttgt aatcaaacca
    27421 cttatccgct tctggcgccc tgatcacaaa atctataaag tttattggga acttttcata
    27481 taattcttct ttaatttctg gtaaatgata agtattatta atcagttccc aaatatcaaa
    27541 acttaaacgg aaatggtcca tcaaatataa aagaagggca tcacgtactt cttcaaaagt
    27601 atcaaaattc ctacacactt cttcctgaaa taattcttcc caggcatcaa tattatttct
    27661 tctggataag cttctgtaaa gagaatctac tttctctatc catctgctaa caggtgtatc
    27721 ttctttttct tcctcttcga ttttactaag tcttatggct tcttcataag cctctcttaa
    27781 aagcttaaaa ccctcagggt catcttcagg attcgtcaca agaagctttt ttctataggc
    27841 tgctttaata atagattcat cttttgtttc ttgtatttcc aatatattga ataccttctt
    27901 cttatccatt ttgtaaccac cttattaaaa attatatgat agcattatac cattaataaa
    27961 ataattatca catatgtatt ataaaatcaa atttttatat tatttttcta tttagttctt
    28021 ttctacaaga tttacacaag attatataat aaaacttatt ttaaaataat agattctcaa
    28081 aaaaaaacta catgttaaat atctctttca tacaatttaa caatgtagtt ttgttattat
    28141 catattttct atcttcccag taaacctcta agtacaagac ctaagccagc agccgttaaa
    28201 ccaaacatga gtattgtttc atataaagta actggcataa aagtaattct caaacccatt
    28261 agtaacccta acaaaatgat aattattcca aaagcaataa aaaaccacgc aagctttgac
    28321 ttagtattaa aaaacaacat gataatccct atcagaagtg gtaaaagaac aaagccaaat
    28381 gatggtgtaa aacccagtat tctacttaag gaaaagccag tacttataat tgtattctgg
    28441 aatattaaat aaattcctga acacaacaag atgagtccta aaaaaaagtt tcctaagctg
    28501 ctcttggagt gttcttcctg atatacttta taagatgatt tttgtttatt ttgaagttcc
    28561 ttcttcttat ctgccataat tcccctccga ataaaaaata acaaataaag atttttattc
    28621 attataccac aaaaatcatt ttatccaagt ataataatta taaaattata aagtataaaa
    28681 aaattaactt ccttttttca gtctttccat ttggtcaaag gcaattaaac tatcaataat
    28741 ttcatccaaa tctccgtcta aaatactgtc cagtttatgc agcgtcaagc ctatcctgtg
    28801 atcggttact cttccttgag ggaaattata ggttctgatt ctttcatttc tgtttccagt
    28861 tcctacctgg cttctccttg tttcagcaag ttctttttct tgtttttctc tttcaatttc
    28921 atataatttt gcccgaagaa ctttcaaggc tttttcttta ttttttagct gtgatttctc
    28981 atcttgacaa gtcacaacaa tattggtagg aaggtgagta attcttactg cagagtccgt
    29041 tgtattaaca ctttgtcctc catgccctga agaacggaaa acgtctaccc tgatatcatt
    29101 tatattaatt tctacatcaa cttcttctac ttcaggaagt actgcaacgg ttactgttga
    29161 agtatgaatt cttcctcctg actcggttat gggaattctt tgaacacggt gaaccccgct
    29221 ttcatactta agcttagaat aagctcctct tccttgtatc ataaatataa tttctttaaa
    29281 gcctcctaca cctatttcat tgctgtccat aatctcgact ttccaattat ttttttctgc
    29341 atacatggaa tacatcctga aaaggtctcc ggcaaaaagg gcagcttcat ctccgcctgc
    29401 acctccacgt atttcaacaa taacattctt ttcatcgttg gggtctttag gcagtaataa
    29461 gatttttaat tcttcttcta tctttacaat tttttgtttg ttttctgcca actcttcttt
    29521 taccaattgt ctgaaatctt cttccagatt atcttctaag agttgatggg cttcatctat
    29581 ggcttcttta acctttttat attcagtata cttttcaact aaaggtttaa gatctccata
    29641 ttctttcatc agactttgcc attctgtttg attattaatc acttcaggat cactaatctt
    29701 ttttgaaatc tcatcatatc tttcattaat ttcttctaac ttactaaaca ttatctcacc
    29761 tctattccca aggattattt aataaatttc ccaaaaacta ccctgtctaa gccggctaaa
    29821 tccttcttta tacttatatc agaaaatcca tgacttttaa agatttcagt tacagctttt
    29881 ccttgattat atcctatttc aaaaattaat atccctttat cttttagata agccttactt
    29941 ttttctataa ttttacgata gaaatccaag ccgtcttttc caccatctaa tgccataaag
    30001 ggttcatagg ctttaacttc aacttcaagt cgagaaatat cttctgtcgg aatatatggc
    30061 ggattggata taatcatatc aaatttacta agatagtctt cttgtaagct ttcaaataaa
    30121 ttactttgaa taaaataaac tctgtcatca acaccattta atctggcatt gtattgagcc
    30181 actttcaaag ctggtgcaga caaatccaga ccaaggactt tactcttttt cttataataa
    30241 gcaatgctta ttgcgataca gcctgaacct gtacacatgt ccaaaatcat gctttctttt
    30301 tcatcgataa gctccagagc cttttccact aaaacctccg tatcatttct gggaatcagt
    30361 acattttggt ctacataaaa aggtaaagac ataaattctt tagtattagt taaatattga
    30421 acaggcatac ctttaattcg ttctaaaatt aagtctctat atttttcttg aatagcttct
    30481 tcaacttctt cttttgaatg ggtatacaac tgcaccctcg aaaaagaaaa cagatgtgaa
    30541 agaagcagtt cactatctaa agcccaagtc tttataccct tatcttttaa tagtcctttg
    30601 gcttctctta agagttcatc aatggtcttc ttcatcctcc aaaactccct tcaatgcggc
    30661 aatggctacc tctatctggt catcgtcagg ttctcttgta gtaaagacct tttgaagcca
    30721 cattccggga atactaatca tttgggctgc tttggatttt gaccttcttg cccaccttat
    30781 cagttcataa gatatccctg caattaaagg aacaagaacg actctgctta ctaaacggag
    30841 ccaaaagttc tctacattaa agataataaa agtaatcacg ctaaccaaaa caacaataaa
    30901 aagaaaactt gttccacaac ttttatgcag tctgctgtat tttttaacat tttctatggt
    30961 caattcttct tcttgctcaa aacaattaat tgttttatgc tcagcgccat gatattcaaa
    31021 cacccggcgg atatctttca tcttggagac taaataaatg tatgaaagaa aaatgataat
    31081 tctaattaaa ccctcaaata aattaagaag ccatttgctt tcagtaaaat ttttaaacaa
    31141 cctggatatt aaaagaggcg tgaccataaa taaggcaatt cctaaaacaa aagcaaaaaa
    31201 tatagtaaat ccaataataa aatcttccag ctttttactg ccaaatttat tatctaagta
    31261 cttttcaaat ttagatggct ctgcttcttc tatatcaaaa aattgtgctg aatagctcaa
    31321 agttcgaata ccaataacca tagaatcaat aaaagttaca atgcctctta acaagggtaa
    31381 ttttaataca ggatattttt ctgttaaagc ggttaattct tttttttcta tgacaatttc
    31441 tttatctggc ttccttacgg ctacagcata gccctttggg cttctcatca tgaccccttc
    31501 aataactgcc tgaccgccaa cacttgttct tttcattgat tcactcccta attacataat
    31561 taatcctata aacaataaag tatattacat tagttatctt ttgcaaaaaa aaggttgagt
    31621 acaaccgcct caaccactta ttgttcttga ttaattccat attttttatt gaacatatcg
    31681 atacgacctt tagccgcagt tgtcttttgt cttcctgtat aaaacggatg acaattagaa
    31741 catacttcaa cgcgtatttc tttctttact gaacctgttt tgaaagtatt gccacagtta
    31801 caaatcaccg tagcttctcc ataattagga tgaatatctg acttcatttt ttcacctctt
    31861 tcaaaaaaat acttcatcat tacaatccgt tctctattat aactttaagc ttagctatag
    31921 cgacaacatt cgtattataa catatagctt tttctgtttc aagagtatta tgatatttaa
    31981 tttctcacaa ttttttctcg attagtttaa tgaattcttc gttatttttt gtatgcataa
    32041 gcgtttcaat aatactttct gtcacttctg caggcgtcat ccgactcatg gcttttcgta
    32101 ttccatatac aacttctaat tcattctgct taagaagtaa ttcttctttt ctcgttcctg
    32161 atttataaat atcaatagca gggaaaatcc gcttttctga taattttctg tctaatacca
    32221 attccatatt tcccgtacct ttaaattctt caaaaatgac ctcatccatt ttactgcctg
    32281 tttcaataag ggcagtccca aggatagtta agctgccccc ttcctgaata tttcttgcag
    32341 caccaaagaa tttcttcggc atatgaaggg cagcagggtc aagaccaccc gataaggtcc
    32401 tcccacttgg cggaatggta agattatatg cccttgccaa acgggtaatg ctgtctaaca
    32461 gaataatcaa atcttttttc tgctcaacca gtctcttagc tctttccaga accatctcag
    32521 caacccgttt atgatgctct ggctgttcgt caaaagtaga ataaactact tcaacatttt
    32581 caccacttat cgaattcttc atatccgtta cttcttctgg tctttcatca attaataata
    32641 caatcaacct ggcttcaggg tgatttttaa caatagcatt ggcaatcttt tttaaaagaa
    32701 cggtctttcc tgctttaggc ggtgccacaa tcatgcctct ttggcctttt cctattggag
    32761 caattaaatc aatcagtctg gtagataaat ccaattgatc atattccaaa tggagttttt
    32821 ctgttggata tataggggtt aagtcttcaa aattcggtcg ttttgctgca acttccgggt
    32881 tgtctccatt aacctctttg acatataaca aagcactaaa tttttctcct tcttttggaa
    32941 ttcggatatt tccttttacc aaatcccctg tttttaaatt aaaccgtcgt atctgagaag
    33001 gagaaacata aatatcttta gtgccgggaa gataattttc tgaccgtaaa aaaccataac
    33061 catcaactaa tacttccagt acgccttctt caacaatacc actatcaagc tgctcaatag
    33121 aagaaggaac tttcgttttt tcactggaat tattttctgt atcattttct ttagtaagta
    33181 tttcttcgat taattcttgt tttttatatt ttgtaatatt tttcacaccc aaatccttag
    33241 caatttctct taattctgcg agcgtcttat tgttatattc attttccatt gttaccacct
    33301 ttgtgtttta caattttggt atttttaatc ttttcccagc atataattct ctttcacttc
    33361 tcatattatt atattgcata atctttttat aatattgacc agatccataa aagtccatac
    33421 tgatactcca caaagtatcg ccttgtttaa cggtataata tgtaaactgg tcatcattat
    33481 tgctttgggt attatcagat gaattgtttt gactcgcttg gtttgtttga tttgtcctgt
    33541 ttgcttgatt tgcctgactc gtctgattgc cttgactatt ttgatttgct gtcaggttat
    33601 tctttgtctg atttgtatta cttgtgttta tactgcttgt ttgagaatca ctattgcttt
    33661 gtggactatt attatcttca taagaagatt cttcttgtat ttgaaccttt aatctttcta
    33721 attcaatgtc tacaacagtc cttaactctt gtaacttttg tcgtaattcg ttattctcag
    33781 caattaattc ttggtttttt ttccttaatt cattaatttc actttgtaat aatgcgactt
    33841 gttctgagga tacattgtct tccacaaata atatatcttc atcattattg aaaaaaagtc
    33901 tgactaagct atagccaccc catataacag ctgcaataag cactacactg cccataacaa
    33961 tagctgaagt aataatttta tccataatta tatttttctt cttccttcta cttctatact
    34021 tggatttaat agagggacga agaaaattat tgtgattttc ttcattgttg tcaaaagaag
    34081 caggcttatt aaataattct tctagctcat ctaagatttt ctttttttcc gtttcatcaa
    34141 gatttgctaa attttcaaat tcatcgtctt ctatccaatc ttttatttcc accaaataca
    34201 accccttaac tatctttcaa tttatagtaa gaatatacca ttatttttga gatatgtcta
    34261 caacttcaga caaatgctgc atctacacgg ctgacttggc tgattaataa acctgcattt
    34321 ttttaagtaa aatatttggt tggctttgta aatattttaa atcctcatta tttctggtca
    34381 ataagactgt ctcactgcca caaaaataac agcttaattt tacttgattg gccaaaagct
    34441 ccatttcaat tttattgctc ccgcattcac agctaattct tccctgctca gccagatcat
    34501 gaatgtaatt aatactctca atagccactt caacattatt gcataaatta ggcattccca
    34561 tttctttcaa aatttcattt aaagaatttt catgagaatc caatgcactt ctcaccttag
    34621 tatcatttcc tataaagcca attttcatgt tttttttagg acactggaga atttttaaac
    34681 tgtcagaaaa gaattggttg attttatagg tataggtatg gatgtcgctg caaagaaaac
    34741 aaggaacaga taagattatt tgctttttat catttaaact aagctgaact gaactacgag
    34801 aacacttaca ttgtattttc tcgtctttat tcccggaaaa atcaaataaa gatataatgt
    34861 caaaacaata accgccacaa gctgcacact gaaatacaat ggtttgcagt tcatttatca
    34921 acatccctat cacctacctt taagtattaa ttcttcaaaa acagaagaat tccttcttag
    34981 gtgacagaaa tttgcctaaa gataaactta ccgtcaaaaa gcaataacta atccgccttc
    35041 tcctgcataa gaagaactaa gtccgttcat ttctactata ataatatctt tgaaattata
    35101 tcttttcatg acttcttctt taaattttag agccctctct atacaattac aatgagcaat
    35161 tcccaaaatt ttttcttcca ggtttttacc ttcttctcca ataatactta ccagtctgtc
    35221 aaaagccctt ttatatcctc ttattttttc caccatgcga atggtaccct cttcggttgc
    35281 ccccattatc ggcttaatgg ataaaactga aaccagtttt gcaatgatag gatttaaccg
    35341 tcctgccttc gctaaatgct ccagactttc cagcaagaaa aaagttttca tttcttttat
    35401 ataatgattt actcttttaa caatttccat atctccaata tttaattttg acagttcatg
    35461 tatttttaaa catacaagca cttcccctat agaagcactg agtgaatcaa aaacatgaat
    35521 aaatttatca gaaatttctt ctaaaaacat atcctttgcc agtactgcgc tgttgtaagt
    35581 cccgcttaaa gctgaagtca aggttactac aaaagtactt tcatcccctt catagctttt
    35641 cataaattgt tcgggtgaag ggcaggccgt tttaggcgaa ccttcgtatt ccttcatcgc
    35701 agtcaaatat tcttttatat ctaaattttc atcgtcaata tatatcttgt ctcctaattg
    35761 aagtgttaat ggtacaattt taatattcat atcctttttc atttcagttg tcaaatcaca
    35821 actactatct gcaacgattt ttattttcac ttataaacct cctaaaattt atttatattt
    35881 atattaatat aattacctta atacgacaag tatatatatc ttaaaaataa taagtggtta
    35941 ttaatacttt ataacaacca tcttaagatt atccagttca atcttctcct agtctttaat
    36001 gatgagccgt atattcacat ttccataata tttttcataa tgtaaattat atctgtcatt
    36061 aaaagataag gaggtgccct tatgtctgaa gctcaatcag gattttttaa agatgattta
    36121 ttattatggt taataattat tatcttattc attccttact tttctgggcg aagattttat
    36181 gggtttgagg caaacaatta aaaaatgact tttaagaggc catccttttt gctattatct
    36241 tcttttaaaa aaagaaaaaa tagattagaa taataatata aagttttgga aaaaatataa
    36301 aaataaattt tataagttag aaaggaagat aaaaatgata gcaaaaattg acagagatgg
    36361 ctgcattggt tgtggaactt gcccggatgt atgtcctgaa gtattcagaa tggctgatga
    36421 tggtttggcg gaagtatatg ttgatgaagt tcctgaagat gcaaaagaag ctgccattga
    36481 agcacaggaa gcttgccctg tatctgttat tactgttgaa gaataaaata ttacacgatc
    36541 ttaaagttat aaaaaaggag ggttttagaa atattctaaa agactccttt tttatttgtc
    36601 ataaagcagt tctatttttc tataaataaa aataaaaata tataagcttt ataatctgta
    36661 atgggggtga attctataaa aaaagttctc tatagcatta tttttattag tatggtcctg
    36721 tatgccatgc ttccaaacaa gcctgatatt gcccgctatg aaacttttga cgacactcca
    36781 ttaattacat tttataagga tgaaacaata gatgaagcga taaatgtcag tgctcaaact
    36841 tatccttatc tctatgccaa gggagggatt gttttagatc ggaacttgca cctgtggcaa
    36901 gtaggtgaag atgtcctcca gataaaaaaa gctttaaaga aaataggcta tcctattagt
    36961 accaattcat atatttttga cgaaaatatg cagaagattg tgatgcaatt tcaatcagac
    37021 catgacttaa acagcgacgg aattgtaggc aaagcaaccc ttaatgccat caataaaatc
    37081 ctcattgaaa acaagatgac agctccaacc gttcagacaa ttgtaaccaa tgcaccgacc
    37141 agcggctact ggattgccat taacaagtct tctaacaccc tgacattatt taaaggggaa
    37201 caggtcattg aaaaatttcc tgtagcgaca ggaaaagaac caaaacttac tcctgaaggc
    37261 aaatggaaaa ttaataataa ggtcatcaat cctgcctgga aaaatatcaa aggcggtgtt
    37321 ccggaaaatc ctttaggcta ccgctggctg ggattaaaca ttggcgacgg atatatctac
    37381 ggcattcacg gtaccaacaa tgaaaacagt atcggttatt atgtctcaaa aggctgtatc
    37441 cggatggaaa atgaggatgt caataaatta ttttctattg tgaaaaacgg aacccctgta
    37501 tggattggaa accaaaaaca actcagccaa tgggggattg tgatcaaata aatatctcaa
    37561 gcttactgac ctattctaat aataattctt taaagatatt tccttctaaa tcctttattt
    37621 taaacagatt atcttccaat atcgcataag aattagggct gtcttctttt gggaaagtaa
    37681 ttgaccctgg attcatgata taaatatctc cctctttctt cgcaacaggt atatgagtat
    37741 gaccgtaaac aaaaacatca cctttacaaa gctcaggaag attattttca ttataaaggt
    37801 gtccgtgtgt tataaacaaa cgcctgttat aatataaaat attagaataa gtcgccataa
    37861 tcggataatc taataccatt tcatcaactt cgctatcgca gtttcctctt atggcaataa
    37921 ttttatcttt catttggttt aataaagctg ctaattcctt tggattatat tctctgggca
    37981 gaggatttcg tgcaccatgg tataattggt ccccaagtaa aattaaataa tctgcccctt
    38041 ctttttgaaa aatatccaaa gctttttttg cataataaac tgaaccatga atatccgata
    38101 aaaaaaataa cttcatttct cttcccccta taaataattt tataagatta ctttctgcct
    38161 cttcaatcat tatatacaaa atctttaaat tacaaaatcc tctgactttc tcagaggatt
    38221 atttttatcc gttcttatga tttgactttt tggaacaaat tcaatttttc cgttctttct
    38281 ttttatccgg tattgatttt tttcaaatcc tgctaactga aaaggactga gagataaatc
    38341 ctctataatg ccaatacttt caccagtttt aacatccttg ccttctgcta taagaaaatg
    38401 ctgatatttt tcattccaat aataagtttg gacgagttct ttagaattaa ctaactgata
    38461 gtcctcttct tccggcatct ctttgaggtt agctgtctta gattgataaa agaactgatt
    38521 ttccaatacc ttaataacgg gataaggccc attttcccac tgaattattg atttttcttc
    38581 tatttttagc cataaaggat taggcttttt ggcctgatcc cagctttcat tccaataagc
    38641 aacgtaattt tcaataaggg aaataaccat gttaaacttt ccatcctgcc agagatatcc
    38701 tctgatatag ttgccttttt caaaagcccc taacatttga tcaacaaatt cccgaacaac
    38761 gataaggtct tttccccttt tctctattgg aattgcctgg atttctttaa tctcaaaaaa
    38821 ttcatctaat atatccacgt acttataatc cccattttcc tgttgataga ctacaataag
    38881 tgacttttta ggagccagaa aaagagttaa taacaattct tttttagaat ctcccaataa
    38941 atctgccttt aaaatattca gaccaatttt atctttctta tcatgccatt ctgtatagtt
    39001 tgtatccttt aatacaattt ctaaaatatt ctctttattg tttatctggt cttttccttc
    39061 tatataatcc ttaatccatt gagcctttaa ggttctatta gcttcagcct tatgcatcac
    39121 aaaaaataca ggaatcatca caagaatcat taagaaaata aagacctttc ttttatgccc
    39181 ctgcatattt ttcccccata ttataagctt atttatttat atgagggcta aaataaaaaa
    39241 atgtctacat gaaatagaca tttcataaaa aacaacccta tccatttaat ctttttccag
    39301 atgatgtctt aaatgcattt tagaattata gttggtcctt ttaatgtcaa tagcatcctc
    39361 tttgttctta tctttatcta aactgctgta ttgaccaata ttatttttta atctcatgga
    39421 acattcttta caaaatcttc ctgttcgtat ttccacacca cacgactcac acgttaacca
    39481 ggcaggagaa tcttctgcca gttctaatcg ttcttctttt aagaacttgg tgattttttt
    39541 tacagacact ccagtttcct tagaaacttc agtaatagtc gcttttgggt ggtcataaat
    39601 gtatgctttg acaagttcaa aatccttttc atcctgctgc acacattcat tgcatatcgg
    39661 cttccccaca taattaaata acttaccgca tctcctacaa ttcctcaact gcattacatc
    39721 acccctcttt tattatacca tacaataaag aaatatttaa taatttggta taatttaaag
    39781 agggttttta tattaaaaaa attttttatc ttatcttagc aggtaaacct atttttcttt
    39841 gaactttgct aagcgtctta tatgcaatac gggatgctct ttctgcattg tttttaataa
    39901 tggaatcaat ataactttta tctttttcca attgataaaa cctttcctgt ataggtctta
    39961 attcttctac aaccacttca gcaacttttg cttttaattc cccataacct aaagaagaaa
    40021 atctttctac aactttttca ataggttctt cagtaatact gcaataaata tttaataagt
    40081 ttttaatacc aggttgctca tccctataag ctattttagc ttcggaatcg gtgacagcct
    40141 tttttatttt tcttaaaatg gtatctggcg agtctaaaag cgtaatatag gaattttcat
    40201 tttcatcaga cttagacatt tttttcgtag gatcctgtaa actcataatt cgggctccta
    40261 ctttagggaa gtatccttct ggaacaataa aggtttcccc ataaaggttg ttaaagcgga
    40321 tggcaacatc cctgcaaagt tctaaatgct ggctttggtc atttccaaca ggcactaaat
    40381 tcgcctgata aaggagaata tctgcagcca ttaaaacagg ataagtaaac aaaccagcgt
    40441 taatattttc actatgtttt tgagatttct ctttaaattg tgtcatgcgg tttaattcac
    40501 ccatgtaagt atagcaattt aatatccatg ctaattctgc atgagcaccg acatgagact
    40561 gataatatat gatatttttt tcaggatcaa gtcctgcagc aatatagagc attaaaactt
    40621 ctctggaacg tcttcttaat tctgccggat cctgcctgac tgttaaagca tgaagatcta
    40681 caatacaaaa tacgcaatta tattcatcct gaagcttggt ccaattcttt aatgcgccta
    40741 aataattccc taatgttata tttccagaag gctgcatgcc actaaaaata accttcttat
    40801 ctttttgatt ctctgctgtc atttgttttc acctcattta tttttaaaat ccacttaaat
    40861 aagtatataa cgttaaagtg aaaacatcaa gtagttgtaa gtttcatcct ttctcaaatt
    40921 tactgggata cggtaaattt gtttaaacca gcacgaatat atacctgatg caataataca
    40981 acaaaaatta catttcccac agcagcaaaa agaacacctg caaataatgt accaaaagca
    41041 cctggtaatc caacgattaa agccgcactg cctaagaaag ccaagccgct taaaagggtt
    41101 ccaataaaac ctataatacc catttttacg ctttcaggaa cagataattt tttaagggca
    41161 tagatcatta agcctacaac taccccagta atgcatttat cgataatatt gggcaactgt
    41221 cctcccggaa agtttgtcgt catggcagaa atgataccgc aagcaataga agttgcagca
    41281 atattggttg ggttaaaatt aataaaaata gctaaaaaca tcatagataa caagaaatct
    41341 ggtttaagac ctgcaaacgt tcctggtacc actgcatgta agatagctcc taaagctaat
    41401 aagattgcgg atgttgttaa ttgttttact ttcattttaa tttccccttt catttttcat
    41461 tattgttaat taaataaaaa aagcatttca tccctgcaaa gggacgaaat gctattattc
    41521 cgcggtgcca ccctaattag atagtaaact atctctctca gccagatacg gagtaagtcg
    41581 ttcttactcg atatcctgct cctgtaacgg gaagccccgg cgaccaatac tttctcataa
    41641 gagatttcat ggtgcttctc atggaaccat tcaatataag tctctatatc ggacctcaca
    41701 ccaaacgccg actcgctgaa tagattctta tatctactct ttccaatcat tgaatttaaa
    41761 gatatcttag atgtcttata tgatatcaca tctgttgaat cttttcaaga acttatacga
    41821 agcttttata aatttattta gtcagctctt taagactatc cagcattgtt tttactttct
    41881 ttttggatgc cacaaagtct gatacgccat ctctgtaaac tgtgtagaaa tcatcatcat
    41941 aaggtacata ttccacagta accttttctc ctgaattaag ggtaaaagta gttcttattt
    42001 caggctctgt cttttctacc tctttatcca gttcggcgtc aaaggttata ccaattaagt
    42061 tttgataata atctttaaaa gcttcttctt ccacttcttt accattgact ttataagtag
    42121 taatggtttc ttcttcatcg ctgtcctctg actttttcgt cgttcttgaa agagtcagtt
    42181 tatcaacttt accttcatgt tctatttcaa tagcatctat taaattaata tcaataatgt
    42241 atacaaacct gtcaattaaa tcgatgggtt ttaagtccag caaaggttga agcttagaaa
    42301 aagccattgt aaagactgtg tccttatcag ctgtcttagc atataccaaa ttatcatctt
    42361 tgtttgaacc caccagtaaa tgcaaactat tactggtatc cttaatctga atttctattc
    42421 tgggttcatc aagaccatat tttgaaagat cggatggctt atcttctaca aattcttcga
    42481 tagtaaattc aaaacctttt tctaaaagtt ccataaaatc tttggaacca acagcatatg
    42541 tgtgattata aggctgcttt agcatccaga ggtccaagcc atatttatta agctcttcat
    42601 tgatattcga aacaatctca atgacaggtt tatcttcctg tttaatatat aaataatcta
    42661 attccagtgt attaatggct gtcagctttt tattgcgtaa atcatcaagg cctgaactaa
    42721 agttttcacc gtgattcatc caaacagtat aaactttagg atcatctttc ttcatcaagt
    42781 aataggtggt tccttcaggc gttttattgc ccaaatagta ttctcttgta tccccgtcat
    42841 caaaaaatgc ttttactgtt gcaacaggcg atttgaggcc atacttttct aaatcctgag
    42901 gattttcttc aactattctt tctgcaaaaa taactgcaaa actataagct aagtcattca
    42961 ctttatcctg gtcaagttct atatcctcgt tacctgtcaa tacccatata tcatcttttc
    43021 tttcgatggt tatttctccc tttgaagaag tcaaaatcat cttagtaatt ttatctctgg
    43081 aagtattgga tatcgttaat tctgaatccg tttcttcttc aataccattt tcctgagatt
    43141 gatgattgat atacagataa gctcctaata aaatgactaa aactaaagca gctataataa
    43201 tattccgaga cttcctcatc tatttctcct cctcagccat acagtcagcc caataatacc
    43261 aatgataaga ggaataagca ctacagtaat acctgcataa atatacacct gagatacatt
    43321 tattttaaga cgcgttgtaa gtaagctctt tggtctaata gaaatacttt cttctttgtc
    43381 cactaaccaa ttgactgtat tcatgaaaaa gtcgatatta ccgcctgcac tttgggcatt
    43441 aattgtttca tttaaaaatt ctgtattccc aacaaccact aatttagtaa tcttttcttc
    43501 attattttca taccaggtat ctgtaacggc cacagcaata tccagaggac catctatgtc
    43561 tccttcttcc ttttctgcca cttcagactg gaaattcgtt tttgcataag aagtttcgga
    43621 tgacgttaaa agaggactta tttccagaga atccctttta acttgaagcg tttcaatatt
    43681 ttgagcaaca gcaaccaaaa caggaagccc gctggacttc aaaggatttg taatttcatg
    43741 tccctgtaaa tttggaataa tgtaaagagg atttgcaaat tgatatttcg catccatttc
    43801 caatatcata gattttctga tactgagtcc atacttttta agtacttcct gaagattagg
    43861 catttgatct tctttcacat tcgtaagaac attggtaaga ataatagctc ttccttgatt
    43921 ttccaggaac tctgtaatct tagccccttc ttctttggaa atatctctta caggagcata
    43981 tatcattaaa atgtctgcat cttcaggaat ctctttatcg gttaataaat ttaaatcttt
    44041 aatctcataa ttttcctttt ccaattgggt tttaacataa taaggaaggc tcggttcatc
    44101 atgacctttt aaagtataaa taacaggatt tttttctgaa ataatatatt gaatggctcc
    44161 ggtcactctt tcttccactg ctaaggattc aacaatataa gaaccgctgt tgtaactata
    44221 attaaataat tgagaaggcg gaataatctt aaatttacca ttttcacctt caacaattaa
    44281 actgtcatta ctaatccctt tgccttcttt atcatatttt gttgcaaatt gaggatataa
    44341 aaccgggtct ttatattcta ctttaatttt attagaatga gcactgtatt gtttcagggt
    44401 ttcatcaaaa ataagatttt ctttaccagt ttctgttaaa acgtacactg tgatattttg
    44461 atccaaacga tctaatagct ccagcgtttg ctgagacagt gaaaacattt tattttcagt
    44521 taaatctaat ttgtaatcaa actgtcccac catcagattg atgatgataa ggatagcaac
    44581 aacaaaagct gtcatcattg aagcatatcc accatatctg actcttttat ccttaaaaga
    44641 ctcccatata ttattcttca ttattgcttc ccctcctagc tccatctacg cttttcaata
    44701 gtttgtatgg ttaaaaagat aaatgcacat gaaaaagtaa taaaatacac aacagaacta
    44761 acatcaaaaa taccatagga aaagctctga tacctttcca ataaagaaaa ccactggaat
    44821 attcttacta taacaccgtc atataaagcc gggtttatta aatacaccgc aaccattatg
    44881 atggctccaa taatgactat agctgcactg ataaaaatat ttttagttga attataaaac
    44941 agagctgcta ttgcaataac gagaattgcc ataaatacta tggaagactt tctgtctgtg
    45001 ggcaatccca tgataattcc ttccataatc cataaaacca ataaagtaat tactgtaaca
    45061 acagcagata ctacctggtt gtcagtcagc gaagaaataa acagacctac agaaataaat
    45121 gccgctccaa gcaaaaagaa acctatgtat cctcctacaa tttctcctac agcaattttt
    45181 ccatgaaaac ttaaaatgac tggatataaa acggtaatga ataaggttaa aaggaataat
    45241 aacaatgctg caaaatactt accaagaaca atttctgata ctttaagggg tgaagttaac
    45301 agcaactggt ctgtcttttg tcttgtttcc tctgcaatca atcgcatagt aagcattggc
    45361 acaacaaaaa gaaaaataaa agttatactg gataaaacat tattatatac cggattttgt
    45421 tcaaataagt tggttattga aaagaagata ccagaaatta aaaggaaaaa gctcataaaa
    45481 atataacctg tagcagaagc aaaataatta cgaagctctt tattaattat tgctaacatc
    45541 ttccccatcc tccctttgtt gaccatcagt ttcttcgtgt tgagcatcat tttcctcaga
    45601 aatgctgata ctttcgtggt caattgcttg ttcctgagtg gttacctgta agaagatttc
    45661 ttctaaggtt aaatctaaag atttcatcat taaaatagga taatctgttg atttaaaggc
    45721 atagaataaa tcttcacgga tatcaagacc ctctttacct tcaacaacaa tatctatggt
    45781 attgggttct ctggaaccag ttatttcagc cgttttaaca gatttaacac cttttacaat
    45841 atttaaagct ttatcttttg aagccttaat acgcagcagc attttgttgc tttgggaaag
    45901 tcgttttgct aaattctcag gtgtatcaat cgcaacaatc ttgcctttat taataattaa
    45961 aactctgtcg catactgcac taacttctga caaaatatga gaactaagaa taatggtatg
    46021 atctttacct aaactcttaa tcacattacg tatttcaata atttgcttgg gatctaaacc
    46081 tacagtaggt tcgtccagaa ttaaaacctg aggctctcca atcatagcct gggctaaacc
    46141 tactctttga cgataaccct ttgacaagtt cttaattaat ctttttctca tatcagttat
    46201 cttaaccgca ttcatcactt tgcccaaaac ctctttttgt ttttgaggtt ttactttttt
    46261 aatatgacat acaaatttta aatattcttc taccgtcatc tccatataaa ggggaggcaa
    46321 ttcgggtaaa tagcctattc tcttttttac ttcttcaggt tgttctaata tatcataacc
    46381 atctacttta actgttcctt ctgtggcaga aatataacct gttataatat tcatggttgt
    46441 ggattttcct gcaccattag gacctaagaa acctaatatt tcaccttttt ccacagtaaa
    46501 actgatgttg tcaattgctt tatgctggcc atactgcttt gtcaaattga taacttcaat
    46561 caaagtactc cctcccgtgt ttataattgt aaaatagttt atcacaaaat cttgaactaa
    46621 atatgaataa attataaata cttttagaca attgttcaag cttttacaaa attataagga
    46681 ttgaacttta aatttcacgt tattgcaaaa aatgcgccta taatagaata taaatatttc
    46741 ttttaaaaat aaaatttaaa ataaatctta taaaaaatca atattaaagt ccgcctttgg
    46801 cggactttgc gtacacactg aaatctataa atctacaggc ttttttgttg tatttgtttt
    46861 ttatttctct taaaaagctt tttaagtttt tcagctatat cgtcgaaaat tgtataattt
    46921 aaaggaatga ccactaaggt taatattgta gataaggaca aaccgcctat catggctgtt
    46981 gccagaggcg ccgttatttc tcctccttca cccagtccta aagacatagg tatcataccc
    47041 agaaccgtgg taagtgttgt cataagaatc ggacggagcc tggttggtcc tgcttccaga
    47101 atagcctctg ttctgtcata gcctctgctg cgaagttgga taatataatc gattaataca
    47161 atcgcattat taacaacaac tcctactaaa ataataaaac ctataaaaga agacatattg
    47221 atggttctcc tggttataaa cagagcaaga atcgctcctg taagggaaat tggcactgaa
    47281 aaaagtattg taaaaggatg caaaaaagat tcaaactggg aagccaaaac catataaacc
    47341 agtataacag ccaatatcaa tgccaaggaa aggctttcca aagaagacat catctgctca
    47401 atttctcctg aaaattcata attatagcct tctggcatat gatatttttt taatttttct
    47461 tcaatatctt tcttaactga attaatatct cttccaaaaa cggatgaact tacggttacc
    47521 attcgcttct gattgtctct cataatactt acaggacttt tgttaatcga gatatctgct
    47581 atttctgata aaggaatact cacgcctaaa gctgatgtaa cagaaatatc tttaatgtct
    47641 ttaaggtatt ccaattgaga actgtcatat tgtatacgga catctatttc tgtaccattg
    47701 actttatatt ttgttgctac cgaaccttgt accgccgttc ttaaagcatt ggatatggtc
    47761 gtcatattaa aaccatacat cgatgcctta ctcctattga taacaatttg agcttccggc
    47821 gttccttctt cgactgaatg gctaacttct ctggtaccat ccacacttct tacaatttct
    47881 acaaaatcat ttgctattac ttccaattgt tcaaaatcat ctccggaaat atctatagat
    47941 atgggtgaac taccagataa gcctgccata aaactgctgc ctgttacaga tatctgggct
    48001 cctggaatat ccttcgttaa atttcttacc tcatcggcaa tttcatcaac ggaacgtttt
    48061 ctatttttaa cagagccaat attaatggtc atacttgcac tattggaggc cgtttctgta
    48121 ccaagcagtc ccgctcctcc cccaatattc acaaagactt cttgaatttc cttaatctct
    48181 tctattcttg tttggatttt ttctaccact tcaaaagttt cttctataac tgttccttta
    48241 ggtagctcaa tattaattgt aaattgcccc tggtctgtag cagggaaaaa ttccattccc
    48301 accagtccaa atattggtat gcttaaagta gctgtaaata cagtaagtgt aatccctaca
    48361 gctctttttc tatggtgtaa ggtccaatta agaactcttt tgtaaaatac atcaacctta
    48421 tttaatcctg ctgcccattt atctaaaatt ttagttatca gtttatttct cttaatctca
    48481 tcgacttttt caactcttaa tattttagaa gccatcatag gcactaaagt taaagcaaca
    48541 attaaagaag caattaaaga aaaggttaca gtcaaagcca tttctttaaa tatctttgct
    48601 gcccagcctt caacaaacac aatgggcaaa aatacaacaa ttgtagtaag ggttgaagca
    48661 gtaactgcta gaccaacttc tgatgttcca atttccgctg cgtccactct gctttttccc
    48721 ttttctctat gcctgtatat attttctaaa acaacaatgg agttatctac aagcattcct
    48781 acccctaagg ctaagccccc taaagaaact atgttcaggg ttagatctac aaaaaacata
    48841 aatgcaaaag taacaatgat tgaaatggga atagaaagcc cgataataaa agtacttctt
    48901 atgttacgta aaaatacaaa caggatgaat atcgcaagaa taccacctat aatagcagtc
    48961 gatgcaacat tttcaactga catattgata aattcagcac tatcataaat aatgcgtatt
    49021 tcaatatctt ttaagtcagg atcttctttt agctgactga tttcttccat gattgctctt
    49081 gagaccttaa cagtatttgc tgttgattgt ttttgaacag ataaactgat actgggattg
    49141 ccattgatgt atgcatacga attcattttc ttataaactt cttcaacttc tgcaacatct
    49201 cttaaataaa tgatgcctcc cgatgtcgtt ataggaagat ttctaatttc atcaatgttg
    49261 ttaaattctc ctatagaacg aagggtaaga gtcgtctttc cttgtttgac ttcgccagca
    49321 ggaagattta aattttctaa tgctaaaaat tgtgcaatgc tggcaggcgt cagattataa
    49381 ccttttaatt tatccggaag caaggtaaca cggatttcct tttcttgtcc tccgcttaaa
    49441 ctaacagaag ccacaccttc tattttttca atttttccaa tgacattgtt ttgaacggtt
    49501 cgtgttaaag cctctaaatc tttatttcca ctaatgccaa tctgcataat gggcatcatg
    49561 ttaggatcta attccaaaac aagaggctca tcggcatctt ctggcaaata accttttatc
    49621 atatcaattt tttctctcat atccagcatg gccatgtcca tgtctgttcc gtcattaaac
    49681 tccaccatga caatagaaga accattggaa gaaatcgatg taatattttt cgcattgctt
    49741 accgttgcaa ccgctccttc taatggcctt gttaccaggg tttcaatttc atgaggtcct
    49801 gcaccactat attgcgtcat tacaattgca tagggaagac taaactgagg taataagtcc
    49861 actggcagtc tccttatgga aatcgttcct aataaaacag caattaagat taacatgaca
    49921 gtggttactg gacgctgtat cgacattttc gaaagcttca taactgattt cctcctatcc
    49981 tattgctcaa caatttgaac ttttccaccg tcaagcagat attcctgacc ttttataaca
    50041 atttccattc cctctttcaa accttcgagg attgcagctt ccttaccatt atcaaggcca
    50101 agtttgactt ctatcttttt tgccctgtct ccttctacaa tataaacaaa atctctgtct
    50161 ccttcagtca atatggcttc tcttggtata acaataacat tgttagccga atcgagtaca
    50221 aattccactt cagcaaacat accaggcttt aataagccgc cttgattatc aatttcaact
    50281 tttaccagat aagtaaaggt tctttcatct gctgccggac tgatggctgc aatttttcct
    50341 tcaaacttat ccgtagaagc agaacggaca tacacatcta ctttttgatt tttttctatt
    50401 ttattaatga tttgttcaga aacatataca tccacaaaaa cctgatcgat ttgtataatg
    50461 gtaaaaggtg caatggcgcc agctaccatt tctcctgcat ctatcattct gctgctgaca
    50521 attccgtcaa tagggcttct gactgccgta tcatttaagg cttcctttgc atttgcaatc
    50581 tggacttcca gactttcttt tgctgctacg gcttgctcca gttgattttg agccaactgg
    50641 acactttctt tagaaagatt attcataaat aaatcataat tattttttgc agcctgataa
    50701 gccagtagag ccatttgata tgcactttct gactggtcta attgctgttt tgaaacagag
    50761 cccatttcat ataatacttt catatcttca taggcttttt tgctatcctg cagttgaatt
    50821 tcagcatttt ttaatgccgt ttccatctgg cttttttgtt gtttatattg acttcctttt
    50881 gcactgctca gaccaatttg ccccatatta accgttgcct gagctgattt taattgggct
    50941 tctaaagctt ttatattatt ctgaagatcc gtttcatcta attgaaataa gaccacgcct
    51001 tctttaacgt agtctcctat atcaaaataa acttctttaa cttttcctgc aaccttactt
    51061 gagactgtaa tctgttgctt tggattaatt ttccctccat aagtaaaagt atccgatatc
    51121 tgacctttag ataccttttg aatatataca ggagtaactt tttcctcaat ttcattatta
    51181 ccacctgtat tgctgcaagc ggacagtaaa aacaccggca ataaaagcac ggatgttaat
    51241 ttcttaattg tcatatccta ctcctcctaa tataattttt tcttaaaaat tattggttaa
    51301 ataccttttt taatttattg ataccttctt caaaatcttt tttcttgtca tcacttaagt
    51361 tttcataaaa ctcttcaaag acaaataaaa catttttatg tacttccata agaacatctg
    51421 ctccctttgg ggttatggat aagtatatct tccttctgtc ttcttcctcg gaaggataag
    51481 ttctcgttaa atagccttct ttgactaatt tagatacacc tatggacaag ctgctgctac
    51541 tgacatttaa ttctttttgt aagtcagaca aagtacacaa cttcagttgg tacaccaaaa
    51601 ttaaaattga aaattgtctg tccgtaatga ttctgttcgt cttaccgttt ttattaaatt
    51661 ctttaaagta ttttccatgg agccaatggg ttaaatgaat aattaaattc agaaattgaa
    51721 gactgacttc ttttgttttt ttatgatcca tatccatcac ctatatttcc ataaaatata
    51781 tttataataa aagtatttta ttataaacat tattggtttg caatcataag aactttttta
    51841 ttttgatttt tattattaaa aatattatga accgcagcca tattttatca ttgtttatta
    51901 atgatttatc ctgcataata taaataaacg aaaggaggaa atatctttga aaaaaatagc
    51961 cagctttaca gtaaatcatt taaaattatt aagaggcatt tatgtttcca gaaaagatgc
    52021 cctgggcagt gaaataataa cgacttttga cataagaatg aaagtaccaa acaaagaagc
    52081 tgtcatgaat acagcagaaa ttcataccat tgaacattta ggtgctactt ttctcagaaa
    52141 tcacgaaaaa tatggaaatc aaatcattta ctttggtccc atgggctgcc gaactggttt
    52201 ttacctggta ataggcgggg atttttcatc agaaacaatt cttcctttaa tcagagaaat
    52261 gtttgagttt attgcaaatt acgatggaga tattcctggt gcatctcctg aagaatgtgg
    52321 aaactacaca gatatggacc ttaagaccgc aaaatttgaa gcaaaaaaat ttttagaaga
    52381 agtactttta aacattaaaa aagaaaatct taactaccct gactaaaagt catatataat
    52441 tcccagtata tctggccttc tatttgtata tactaagata gaaaaatata aaaggagact
    52501 agatatggct actttcccta atccattttt ccgcaagttt acttatccaa agaacagctt
    52561 tttaaggata agttcattaa ataaaatatt tcctcataaa aaccatgtcc tctttaatta
    52621 tgatgaagaa tatgccaaag agaataatga agactttaaa aataaccagg aaaaaatccc
    52681 ccattaaggg ggactttttt attctcttaa atgggctcct aaatttgatt ccagaccatt
    52741 aataattttt tgcattgcct tattgacttc ttcttccgtt aaggttcggt cctgtgccct
    52801 gaaggtaatg gaataagcca cagacttata accttcttga atttgttttc ctttatatac
    52861 gtcaaaaaga ctataagatt cgagaatctt tccgccacgc tgtctgataa tattttcaat
    52921 gtctttaaca ggaatttcat ctttaaccag cattgcaata tctcttgata cagcaggata
    52981 tttgggcaaa ggtttatata ctctttctaa atcagcttct tcaactaagc tggatacgtc
    53041 aataactccg acatataccc tggtattaat atcataattt tcagctacat tgggatgaac
    53101 ttctcctata tagccgattt ttttattctt acaagaaatc gaagcacaac ggccaggatg
    53161 catccaggat aaattttgtt ctaaactata ttctactttt tcagacatac ccaatttttc
    53221 aaagagaact tctatgctgc cttttaaatc ataaaaatct acatttccat acatacctaa
    53281 agttaattta agcttttctt ctggtaattc tttaagcggt aattcttttg gaatatatac
    53341 ttttcctatt tcataaagaa gaacctcttc atttcttcta ttataattga tagataagga
    53401 agtcagcata ccatttaaag tttgggttct cataatacta aagtcttccc ctaaaggatt
    53461 ggatatggta atgaccttac gtaagtcact atcttcacta atattgagtt tttcaaatac
    53521 tttgggactt tcaaaagtat agttcatgat ttcatagaag ccttgtgctt ccattgtaat
    53581 tttaatcagg tcttcaatcc tttgactata agtctttttg ccaaccgttg gctttcctct
    53641 ttccaagctg ggcgtaatct tatcgtaacc gtataatctt gcaacttctt cagccaaatc
    53701 ggcattgcct tcaatatccg gacggaaagt aggaatagta accgttttag cctccctgtc
    53761 aactttaaat tcaagggaag taaagtaatt tatgatttca tcttcggata aagtaattcc
    53821 taaaaggcca ttaatctttt ctacagaata aggaatggtc tttgcttccc ttttattagg
    53881 ataaacatca acaatacctt ctaccacttc tccagcgcct aattcaatga ccaattgggc
    53941 cgcccggttt aaaacatcta ttacaagatt gggatctaaa ccttttgaat attttacaga
    54001 agcatccgtt ctaagaccta atttctttgc cgtaaccctg atattggctc cattgaaatt
    54061 agcagactca aataagatag tttttgtgtc ttctgttacc ttactttctt ctcctcccat
    54121 cacaccgcca acagcaatgg gattttctcc atcagtaatc acaagcattg tttcatccaa
    54181 atctcttgtt tccccatcca gggtcatgat tttttctcct gcttttgcat tacggacaag
    54241 gattttctta ttttttaatt tgtcataatc aaaggcatgc atgggttgac ccagctcaag
    54301 cattacatag ttggtgatat caacaatgtt attaataggt cttatacctg ccgcaagcag
    54361 tctctgacgc atccatttag gtgatggttg tattttcaca tttttaataa ttcttgctgc
    54421 ataacgggga cataattctg aattggcaat ttctatggat acataatcag aagccttgcc
    54481 accactttct ttaacattta tttctggata cttaaatgga agtttaaaag ttgctgcagc
    54541 ttctctggct atccctacaa cactgaaaca atccggacgg ttggatgtaa tttcgtattc
    54601 tacaacaata tcatttaatc cgaaaacttc tttaatatct tttcctaagg gatatgcttc
    54661 ggataaaata tagatcccat cttctggtgc atcaggaaaa tcttcttttt caaaacctaa
    54721 ttcattaatg gaacacatca tgccttggga ttctacacca cgcaattttc ctgtttttat
    54781 ctttatacca ccaggtaagc tggcaccatg aagagctata ggaacataat ctccttcttt
    54841 gatgttatgc gctcctgtga caatttgaag gacttctgtt cctacatcga cttttgtcac
    54901 caacaattta tccgcatcag gatgcttttc aacagataaa attttcccaa ctacaacatt
    54961 acttatttct ttaccttgtt caacataatt ttctacatta gaacctgaca ttgtcattgc
    55021 atcagcaaaa gtctttatat cacagtcaac aggcacataa tctttaagcc aggacatagg
    55081 tatattcatc gccatactcc tttctaaaat tgttttaaga aacggatatc attttctaca
    55141 aacaaacgta aatccttaat accatatttg ctcatagcta atctttccaa acccatacca
    55201 aaagcaaaac ctgtatattc ttcaggatct attccagcca tacgtaaaac gttagggtga
    55261 accattccac aacccagtat ttctgtccaa ccgcttcctt tacaaacatt acagcctttt
    55321 cctccgcaca caacacaaga gacatccatt tcagcactgg gttctgtaaa ggggaagaaa
    55381 tggggtctga atcggacatt tactttttca ccaaataatt ctttggcaaa cacttctaaa
    55441 actcctttaa ggtccgcaaa ggtaatattt ttatcgacca ctaaaccttc tatctgatga
    55501 aaaacaggtg aatgggttgc atctactgca tcggaacgat aaacctttcc cggacatatg
    55561 atacgtacgg gaggtttttg tttttccata acccgaactt gtataggaga ggttgcagta
    55621 cgaagcataa attcctgatt gatataaaaa gtatcctgct catcccttgc cggatgatct
    55681 ttaggaatgt ttaaagcttc aaaattataa taatctgttt caatttcagg accttctgca
    55741 attttatagc ccatgcccag aaaaatctct acaatttcat ttaaaactaa attcatagga
    55801 tgacgaccac ctgtttttct cttcctgcca ggcatggtta catcaataac ttcttctttt
    55861 aactttttct cttgctcttt tagaatcaaa agatttttta cttcttcaag tttttgttca
    55921 atctcatccc ttatttcatt agccaattgt ccgataattg gccgttcttc cggggataaa
    55981 tctttcatac cccttaatac ctgggttaac tgaccctttt tccctaaata ttctactctg
    56041 atttcgtcta atgctttaag ctcttttaca tcatttaaaa gacttatggc ctgatttctt
    56101 atgttctgca acttctcttt cattacaaaa ctcctttctc aaaaatttaa agcaccatta
    56161 aaaaactccg tcccaaaaag ggacggagca aatgtccgcg ataccaccct agttcctgct
    56221 atttaaacat aataatagca agcactcttg tcctttaacg gtggaaaacc ggcacaacct
    56281 actaaccagt tcagtcatgc agctccggtg ggaatttcga tagcaattga acctaagaat
    56341 gctttcagcc tatggcattc tttctcttta aggaaatttg ctatctactt agacgctaaa
    56401 cgtcttgcac cttcatagct tttatcattt atgatacatt ttaacacatc cttttttatt
    56461 gtcaagacta caaaaaatcg gattttgcaa tccgattaat ttgtatttgt attagaattt
    56521 gtaacagcag aattcttttt aaagtaaagg tggtatatca cacagccaat caagataaag
    56581 ggaagggctg aaatcaccag cgccagtaaa ccaaaagcta atgaaaaact aagaccaatc
    56641 agtttaaaaa gaagcccgat ggctttaaat aacaaaggaa ctccaaacac tacagtaaca
    56701 acaccaataa ctaaataaac aagcattctg actaatctat tttccatatt cttcacacct
    56761 ttcgttttgt tttttatttg cctatatata cgcaaaaaac aaaacgaagt ttgaaaaaaa
    56821 cataaaaatt gttcaaaaca gtttattgtt taaacttatt atgatttgta ttctatttcc
    56881 ccagctttag tcagggcaat ttttgaagca attggtccta ttaattcaaa aactaataca
    56941 ctgcaaagaa ccactgcccg cacttgtgat gaatattgag gtaaagcctt gtcaactacc
    57001 agagaaagtc ctatagcaac ccctgcctga ggcagcaagg tccagccgac atattttttg
    57061 accgttgata cggattttgt aagtaccgcg ccaaaccaag ccccaaagac tttaccaaat
    57121 atacgcatta gaatataaat aactcctatc accccgatag aagggataat cgagacatcc
    57181 aattccgcac ctgataatac aaaaaagagc ataaagacag gcggtgttat actatcagca
    57241 attttaaaaa ttaaattggc ttctttctct atattgacca gtacagcacc taaaaacata
    57301 catgcaagca aagcagatgc tcccagataa tccgccagtg aggaagtaat aaacaaaatt
    57361 ccgatgattg cacacattct attggattct ttcttaaaaa agtgaagagg aatcaataaa
    57421 agtaaagcaa gaatcagtcc tagagcagca gacagagtaa gttctataaa aggacttaac
    57481 agtgataata atacacttcc accagcttca tttgatgaat taataaaggt cagagcaaaa
    57541 ccaaaggcaa tcaaagcaat ggcatcatca atcgcaacaa cactaagaag tgttttagta
    57601 acaggtccat ttgcctgata ttgcttaata accattacgg ttgctgcagg agctgttgca
    57661 gatgcaatgg ttccaagcat tacagcaagt gctggatcca aaccataaat aatcaatcct
    57721 aaagcaacaa aaacagccgt taagatggct ccaaaaaagg ctataacaat aggaattatg
    57781 cccatttctt ttaagtaaga aacactaaac tcagaaccta ttgaaaaagc aataaaagcc
    57841 aaagccattt cagaaacaac actaatatgc tcaacggtct caaaagacaa tatattaaat
    57901 acatatggtc ccatcaaaag acctgcaatc agatatcctg tcacattggg caaacctata
    57961 aattttacaa ggcgtccgaa taaaagtcca gcaagcagca tgatacctaa atatagaaga
    58021 atgctcattt gtctaatccc ttccctctaa caaaatccag aggaagcgta aacataatgc
    58081 ccgtatcggg tgctgaaaaa tcccctacga cttcttctgt aatacgtaca accgtttctt
    58141 cctggtcatc tctgatgact gttaagatgg tcttactttt tttattttca tctccgctta
    58201 aatatctgcg aatagaacct aaaaaattaa tttcttcttc atcatgttta aaatgataaa
    58261 gttcccggac catgcctgta ctttccagaa tagttgcccc ggctattccc tctttaagaa
    58321 aagtcatcat caggtcattt aacttttccg tccggtttag gattaaaaca aataacttca
    58381 tatatttaac ctccttatta aataataaat aatccacctt atatacaacg atgttctaaa
    58441 aaactacatt tttctaaata ttttttataa atatgtttgt atttttcaaa aaattattta
    58501 taatgataat ataagtagtt ttcataagca taatgccatc tgtctaaata aatcataaag
    58561 aaggggttct tatgcttaat aattttttga ataagatgct tctaatcact tctattttaa
    58621 cactgggaag cattttattt aaaaaatatt tatttcaaca aaataaatat aaaaaagaat
    58681 tccttggatt cttcggagga accattggga tattattttt tgcttatccc gatttaatac
    58741 atgataatgc cctatacata ggaacccttt ggattatcct ttccgctacc agcttatttg
    58801 gtaatttttc ttcttccctg attgcaggct taatgcttct ttgctttgac ttaatctatt
    58861 tcatgtcatt agatattacc atcatcagtc tattattcat tctcatttgt ggaatcgccg
    58921 atatcttacc tatacgccaa aaatacaaat ggctggtctt aaacagttcc tggtttctta
    58981 tatttttatc ctattctaaa tctttgtcaa tacctgttta tcctttttta atgatttgta
    59041 ttacagcaag ttcttatgtt cttctgcttt tatatcttat acgtaaaaat tacatcagct
    59101 atatgaatct taaaattcag gctacaaaag attatttaac tggtcttaac aataaacgtc
    59161 agttcaatct gatattgaaa aaaacttttt ctaatcttgg gaaaaaggat aaactggctt
    59221 taattatgat cgatatcgat tattttaaat atatcaatga tacttatggt catccaaccg
    59281 gtgataaaat cttaaagcaa tttacagaag ttttaaaaaa gctttgtccg acgaataata
    59341 tttcaagatt aggtggagaa gaatttggca tcttactttc caattattcc ttatcaaaaa
    59401 cttacgattt agcagaaaaa atccgttcag gcattcaaga aagtcctttt aaaattgatg
    59461 aggaaaatac aataaatatt accgtatcta ttggtatatc cgtttatcct gatacggcag
    59521 aagatataag cagtttaatt aaaacagcag accaggctct ttataaagct aaaaaatttg
    59581 gtcgcaaccg ggtcaatctt ccactaaaaa tttacaacat aaaagaacct gcctcttaaa
    59641 caaaaattta agacaggttc tttttaatgt aaaaacttat ttgcttttat cttcctgaag
    59701 tttctgatga attgccctgg ctgccttttt ccctgcaccc atggctaaaa taaccgtagc
    59761 tgctccagtt acagcatcac caccggcata aacattttct ttgctggttt ccatagtttc
    59821 ttcatgaaca acaataccac cccactcttt ggtcaaaagc ccaggggttg tctgacgaat
    59881 caaagggtta ggaccttgtc caatggcaac aatcaccgta tcgatatcga cagtaaaatt
    59941 actgcctttt ttctcaacgg gtcttcttct tccagattca tcggcttcag ccaattccat
    60001 ttcaacacat tcaatagact tgacccagcc attttctcca ttaattttct ttggattgct
    60061 taaaaactta aagataatgc cttcttccct ggcatgaata atttcttctt ttctcgcagg
    60121 taattcttct tctcctcttc tatatataat ataaacttct tttgccccta atctttttgc
    60181 ggttcttgcg gcatccattg cgacatttcc tcccccgatg acggcaactt tttctccaac
    60241 ctttacggga gtagggctgt taggaaaatc ataagctttc atcagattaa ttctggttaa
    60301 aaactcattg gcagaataaa cgccatttaa attttctcct tcgattccca taaattttgg
    60361 aagacctgcc ccgctgccaa taaatacagc cttatagcct tcttcaatca gttcgtcaat
    60421 ggtaaccgaa cggcctacaa ctacattctt ttcgattttt acccctaaat caatgatgtt
    60481 ttgaatttct gcttccacaa gttttttagg cagacgaaac tcaggtatac catacatcaa
    60541 tacgcctcct aaggtatgaa gggcttcaaa aatggttaca tcataaccta gttttgctaa
    60601 atcccctgca caggtaaggc ctgccggacc cccgcctaca acagccactt taattccatt
    60661 tttcttaatg tccggactgc tctttttccc ctgctgcata tgataatctg ccacaaatct
    60721 ttcaagcctt ccaataccta caggttcccc tttaattccc ctgacacaat tcttctcaca
    60781 ttgattttcc tgagggcata ccctgccaca aattgcaggc aaattatttt cttttgttat
    60841 taattcataa gctttttcaa aattgccttg ggttacttct tttataaatt ctggtatagg
    60901 cacattaacc gggcaacctg atacacaagg tttatgctta caatttaaac accttgttgc
    60961 ttcttccatc gcttgttctt ttgtataacc taatgccact tcatcaaaat tctgatttct
    61021 tacctgagga tcctgttcag gcatttttac tttatttaaa ctcatattag gcatcttaaa
    61081 ctcctcccag tccaatacga caaatatgct cttcttcttt atacatcccc tgtcttctta
    61141 tacattcatc aaaatcaact aaaaaaccat caaaatcagg gccatcaacg catgcaaact
    61201 taatttcgcc tcctacagtt accctgcagc ctccacacat acctgtacca tccaccataa
    61261 ttggatttaa agatacagtg gttttaattt tttccggctt tgtaatggcc accaccgcct
    61321 tcatcataag caaaggacca atggctataa cctcatcgta tttatttcca ctgtttaata
    61381 attcttttaa taggtctgtt acaaaacctt tttttccttt tgtcccatca tccgttgcaa
    61441 aatagacctg attgcaaatg gcttcaaatt tatcagcaag gaggataaat tctttactcc
    61501 tgccacctaa aatcacatca acctcaacac ccatttctgc cagctttttt atctgaggat
    61561 ataaaggggc tgcgccaaca ccgcctccaa tccccaatac ccgtttatgt ttttttaatt
    61621 cactggcttt acctaaggga cctacaaaat cctgcagaca gtctcctact tttttttcac
    61681 ttaataattt agtggaataa cctaagatct gataaataat tgtaacgctt tctttttccc
    61741 ggtcaaagtc tgcaattgta agaggaattc tttcaccttt ttcatccaca cggagaataa
    61801 caaattgccc aggttcacat tttcttgcca caaaaggggc atgaattacc attaattcaa
    61861 caagatcgtt gagtttttct ttttctaaaa ttttatacat aatttcctcc atcctattct
    61921 atctcaacca ttttatcttt ttatgtctca tattaaaatt ctattcgtca agtatcaaaa
    61981 catctactga tattgaatga tgtcttttta tatcccctgt taaaatgcat aaaataatta
    62041 aataaaaaag ccctcatgag tcaagtaaaa gggcttttaa tcattataca taatacaaac
    62101 taataaatca ataattggta taaacttgct taaccataaa ttatctattt aaaagctaac
    62161 ccaaaatgtc agacaactcc atatacaaat tttcctcgtt attaatgata agattttggc
    62221 ttttttcagt tgctttatct tcatcaatcg tttttatagg aagcttaatg ataaattctg
    62281 tccctttatc ttcttcacta taaaaattga tcgttccacc atgtagttct actaaagatt
    62341 ttacaagaga aagtcctata ccactgcctt cattatgccg ggttgtcggc gagtcaactt
    62401 gtccaaatct ttcaaaaatc aacttttgtt tttctgttgg aataccaacg cccgtatctt
    62461 ttacggaaat aactgcctga ttattttctt cactaatact tacccggatt tctcctccag
    62521 aaggcgtaaa tttagccgca tttgataaaa gatttaacat aatcctgtct attttttctg
    62581 agtcaaaggc catgataatt ttatcttttg tactattaaa agaaagtttt atggatttat
    62641 tttctatgta gtgagctaca gacatggtaa tatgcttaat aaaacttaca atgtccttat
    62701 tttctaaatg caatcttaca aagccagtat caatttttgt catatcaata agattgttaa
    62761 gtagcctcaa taatctataa caattttgtt tcataatttt tgtatatttt gtaccgttat
    62821 ctgcaggtgt ataatacttt tctaatatct gtaaagatgc aataataaca tttaatgggg
    62881 tttttaactc atgagatata ttggcaaaaa actccgtttt taatttatca tattcattgg
    62941 cttcttccag taatctttcc tgaagaccaa tatgtttttg caaaatcatt tctgatcttt
    63001 tcaaatctgt aatatccctg atatgttcaa tcagaaactg aacattattt tcatcatcca
    63061 atacaggata agccataatg tctacccatt ttttcatcgt ttcaatatat ttttgcactt
    63121 tgtaaggctt tttatatttt aaagattttt gagtcaccgt ttcttgacat ggactaatgc
    63181 acatactatt acactttttt ccgacaatct cttcttgcga ctgcttccaa aagataagtg
    63241 cagcaggatt acactggatt atcttatccg ttttatcttt tatacaaatc ggatcaggta
    63301 aattattaaa aataacattg agcatcgcaa ccgtctctct taacattttt tgtgtttcat
    63361 tataattatt taaatccgtt aaaaaagtat aagaacctgc catatgtatg gcttcatcat
    63421 cttcatccca ttttgctttt ccctgaataa gaacccattc ataagcagta ttgaatttga
    63481 ttctgcattc tattttaaaa tgctgaattt ccttttttaa atacttgttt aaaaaatggc
    63541 ttaatccatc aatatcttga ggatgaatga tatgatttat ggcatcataa aatacataat
    63601 gtgatgaacc ggatatatcg gaaagctcat tgagcttttt actataataa attttattat
    63661 tttttatatc ccagaaccaa aaaccatcat tagctccttc aatcgctaat tccatgcagg
    63721 cattactgga ctgactattg aaggattgat cctcctgata aaaatttaaa ctcatgtcac
    63781 catctcccaa aaagagataa aatctcatct gtatatacac ttctttaaat ttaattatac
    63841 atcatttaaa gaattatgca aattatttta cattatacta tattttttaa ctccagtata
    63901 taaataagat ttttaaaact ttcatgcaaa aataaaagat taaataataa catttgtacg
    63961 cttttaacta taacaaaaaa ttataataaa gccgtgactt atcatacatt ccattatgca
    64021 taattgttta tccctttatt tattcactta ttccatactt ttttatgaaa taacttttat
    64081 ttttcgaaat gatttttaac aaccttatta attgatcgat ttcctgaaaa gtattataaa
    64141 agccaaagct aattctgacc aagcctggtc ttcccttatc atttttcata tagcctttag
    64201 attcttcttc agatatgtcc agcaattttt gaacataggg ctgggcacaa aaacagccgc
    64261 ttcttactga aatagcccct tcataagcta aagcaagagc cgttacttca tggggcaaac
    64321 ctttaatgtt aaaagaaatg attcccaccc tgccactctt ttgaaaggct tctccgtaaa
    64381 taattatatc gtctatttct cttaaacgtc ttatcgcata atcagaaagt tttgtttcat
    64441 attgatatat attttccatc ccaatttttt taagaaactt tatcgcttca gccaaagcca
    64501 caacgcctac tgcattaggg gaaccggctt cttcatttaa tggcgcatca ttccataaga
    64561 catctttatg gcttacaaat tttacagtac ctcctccagg ttcggatggt ctgcctcctt
    64621 caaaaaaatc tttatgtcct attaaaacgc ccgtcccaaa aggtgcatac attttatgtc
    64681 ctgaaaaggc taaaaaatcg atatgttccg gatcatcatg tgctttcata tccactgaaa
    64741 catgagggac taattgagcg ccatcaacaa atatcctggc tccatattga tgggcaagtt
    64801 ttgcaatttt atagacagga ttaatataac ccgttacatt tgaagcccct gtaactgtta
    64861 ccagttttac tttaccctta tatttttcca gtttgtattc caaatcctta atatccagtc
    64921 tgccatattg gtcgactcca atatagtcca aatcaaactg actcctccaa ggtaaatcat
    64981 tggaatgatg ttccatagct gtagatagta caagccctct tccctccttt tgaaaaaggc
    65041 gccttgccat tttattaatt gcctgcgtcg tattgcttgt aaaaatcact acatctttat
    65101 taaaatctgc tcggacaaag tcagccaccc tttcccttgc tttctcataa atccaggtag
    65161 aaactctgga cttgtaacct actcccctgt gaatggaaga ataataaggc aaatatctgt
    65221 taacagcctc cacaaccggt aaaagaggag gtgttgttgc cccattatca aaatttattt
    65281 ctataccaga cctatcatcc attaatggca cttttctatt tatcccaaca attaattttc
    65341 ttaatttata aacattaata ggatttttca taaccacacc tgcccaacat actctatatt
    65401 attttattat cgaacactta aaatgtgctt gggcttagtt taaagtccat taaaaaaagc
    65461 ttttatttac cgtccaggta aacaaaagct tttttacttt atcttattta aaatattttt
    65521 tgcttgtttt tatctaaaag caagattaaa ggataaccta tgccataaca agcaagtact
    65581 tgacctaagc ccactgtaac aatagacatc agaaaaggag cattcattgt aatcttcaaa
    65641 ataattggca caacaattgc gttaacaagc acagggggca aaggaaccaa aaacttcttt
    65701 ggcatcttat aagataatat ggcagcaata agggtggcca aacttccaaa aataatatct
    65761 aaaagagaac ttcctactaa attagatatc agacaaccta taaaaagtcc gggaatagct
    65821 gctggtgtaa agtatggtaa caccgtcaaa gcctcagaaa tcctaaattg aacaggacca
    65881 aaacccataa aaccaaaagt taaggtaatc accacataaa ttgctgcaat ggctgccgcc
    65941 tgtgtcaaaa attttgtgta cttattcatt ttcattcctc ctagttttgt ttatagtcag
    66001 gttggctttc gaactgactg aattacaact ataacatatt ttaggctaaa ataaaagata
    66061 attttaagaa ctaatcgttt aagaaattaa actgtgcctt aaacaaatcg taatataatc
    66121 ctttcttttc taaaagttcc ctatgggtac ccatttcttt tatctttccc tcatcaataa
    66181 ccattatcct atcagcattt tgtatggtag aaagacgatg ggcaattaca aaagaagttc
    66241 ttcctgcaag aagtctttca attccttttt gaatcagtct ttctgtctgg gtatcaatac
    66301 tggccgttgc ctcatccaaa attaaaatcc tcggatttgc cagtaaggct cttgcaaaag
    66361 ctatcagttg tctttggcca acggataatc tggaacctct ttcattgata tccgtatcat
    66421 atcctttttc caacttcata ataaaatcat gagcagatac cgctttagca gcattgataa
    66481 tctcctcatc actggcatcc aatttgccgt agcggatatt atcttttatt gtccctgaaa
    66541 acataaaggt atcctgggtc ataatgccca tttgccccct cagactttcc agggtaactt
    66601 ctttaacatc atacccatca attaagactc tgcctttttg tgcttcataa aaacggctga
    66661 ctaagttaac aatggttgtt ttccctgctc ctgtaggtcc tactaaagca atcgtttcgc
    66721 ccgcttttac ctgaaaactg acgtcattta aaacaatatt gtcatcatcg taagaaaaac
    66781 tgacattctc aaacaccaca tcgcctttta tttcaggcag aaccctggca ttttccatat
    66841 cttttatgtc tgctttaata tccatgatat taaaaattct ttcggcccct gccatattaa
    66901 tcacaaggag attataaaaa tcactgatat taacaatagg tctccaaaaa atcgaaatat
    66961 atcccgtaaa agccactaac aagccaacag taatatctcc gctgtttaac atgctaacac
    67021 caaaccaaaa gactataatt gaacctaagc cccaggaaaa ctctgttata ggccagaata
    67081 aatcattcat ctttaccgca tgaacaaaag ccgtttttaa atcatttaaa tgcgtataaa
    67141 atctcttact ggtatattct tccgctgcaa aactgtgtat cactttaatc cctgaaaaat
    67201 cttcatgaat ataagcattc ataagagaat tcttctttct gtatgtctgc catctttttc
    67261 tcgatacaat ttgtatgcaa aacatcgaca gtaataaaaa aggtaaagta gtcagagata
    67321 acagggcaag ttgaaaattc atagaaagca taatcatgac aacgataatg attttaatca
    67381 tatctggaat caaattggtc acactattgg aaaataagtc atttaaggaa ttcacatccc
    67441 caatgattct tgctaaaatt tttcctgtgg gcctgctgtc aaaaaaagaa aaggataaac
    67501 tttgaatatg gacatacaat tcttgtctaa tcgttaagat aatcttgtta cttacctttg
    67561 ccataatcct tatcctcttt ctgctgcaaa ctaaggcaag aatattggca ataatcatga
    67621 taccccccaa taaaaacagg cctttaatat ctttttgaac aatatattcg tcaatacccc
    67681 ttttcataaa ataaggatta agtaattcaa cagcaatgac aatactcatt aaaaataacg
    67741 tttctgctat ttctaactta taaggtttta aataagcaaa gagcctcata ataatttcta
    67801 tatttgattt ttgagtaatc ttatcatctt tatttactgt cataaacatc acctatactt
    67861 ttcttgtctc taattcagaa atctctttca gacctttaaa ttgttcacaa taaatttcat
    67921 aatacttgcc tttcttatcc agcaaactct tgtgatttcc ccgttctaca attttaccat
    67981 cttccaaaat aagaatttca tcagcatctt ttacagcaga aattctatgg gcaataatga
    68041 ttttcgtaat atcctttctc ttttctaagg ctttttgaat tttatattct gtttccatat
    68101 caagagcaga ggtcgcatcg tctaagatta aaatcggact gtttttaacc agggcccttg
    68161 ctatagagat acgctgcttt tgcccacctg ataatccaag acccctttcc ccaatgagtg
    68221 tctcataacc ctgaggcatt tcttttacaa aggaatgtac ttctgcgtct atcgctgctt
    68281 taattaagtc ttctttgctt gcttcccggt taccaaagcg gatattttcc tcaatggtat
    68341 cagaaaatag gaaggtatcc tgcataacaa cagatatatt acgtcttaaa gtcttaagat
    68401 ccatttcttt tacatcaata ccatctatca aaatagaacc tctggtacag tcataataac
    68461 gacctataag atgaatcagg gaacttttac cagagcctgt ctcccccata atacctatcg
    68521 tatttccggc cttaagttca aaagaaatat cctctaaagc cttgtgacca tcatattcaa
    68581 aatccacatt cttaaatgta atgtttcctt ttatcttttc tggtactaca ggattttcag
    68641 ggttctttat caaagcttct tcattaaata ttttttctat ttttttactt gaagccaaag
    68701 ccctggccaa tagatttgta agccacccca tcagacgcat gggccaaatc aacatataaa
    68761 tgtaattact gaatgccacc aaaagtccta tggatatctt ttcacggata accataagcc
    68821 cccctgccac aatcccgata acaatgacca gattggacaa aaattctatc atggggttat
    68881 agtgcgccag aacgcttgcc tgctctaaat ttaactcata atttttttta ttctctatta
    68941 aaaatttctt tatttcatat ttttctctgc caaaggcttt aaccagcctt acccctgcta
    69001 tgttttcctg agccgttgta tttaaaactg ccccctgatc gctgattttt tcataagttt
    69061 ctccagcttt tctttcaaat ttatatgcaa agaaaccgat cagaggcatt aaagacaagc
    69121 taacaagagc caatttccaa tgaagaataa aaagtaaaca tgaagcaatg ataaaataaa
    69181 aaatttgttc taaaaataac ataatcccaa aactgacagc ttgccagata ttatccgtgt
    69241 cctctttaat tctggacatc agttctcctg tattcatttt atcaaaaaat gaaaaagata
    69301 agctttgaat atgatcaaat aagtctttac ggatatctac aatcgtcttt gcactcaccc
    69361 agtcaaatgt catatcctta acatagccta aaattcctct ggtaaccgta atgataagca
    69421 aacctaaaag ggctccttta aatttttcta aatgtccttc cacaataacc tcatctacaa
    69481 cgattctgct aaaataagga ttaaacatat ccagtattaa gttaagcatc atgacgataa
    69541 aacccattaa gtaaatcttt ctgtatttgt ttacataacg aaataagcgt ctcatcctat
    69601 acctcctaaa aattaagcac cccaggtcag cttatcttat ttgcaattaa aatacatgta
    69661 aaaaagtcat ggtaacagcc atgacctttt taagagataa aaaaggccat aggtaataat
    69721 acccatgacc ttaatatcta tttcaaagga taaattgcta aaacacaacg gctccttttg
    69781 aggcagggct attactgctg gttaaaaaaa caaatataca acttttaaag aattgattaa
    69841 aaacattacg gctataatta ttgttaccat tattactata atacatataa tttaaaatca
    69901 tattcctgcc tcctttcata atatttacat gctatttatg ataatgcagg caaaatgacc
    69961 ttgtcaatag ctttaatgaa aatataacaa agaaaacatt aaaaaaacgt acataaagta
    70021 cgtttttatt ttataactct tactattgga tttaagaaga aaaggcttcc tgatccttta
    70081 catttaactt cttttctctt tctaaaacaa tcacttccac ttctggcgtt gtagaagcta
    70141 tatagctggc tatatcattg gtaagtaacc tgtaaatctt atttttcatg gtttctccta
    70201 ataccaattc cgttatatta tgttctctga taaaagatac aattctttca ggtacattat
    70261 catcacaaac cacgtgaact tcagcaccta atttagaagc ataatcaaat aattcctgga
    70321 gtaactctcc tgtatcattc tttgataaaa tgttattccc ctgttcgacg tgaagtatat
    70381 gtaactcccc acccaattgt tctgctaatt cagcaccttt tttaataagc cttctactgt
    70441 tctgctgaat cgtgatgcaa accaaaatct tttgtttcat aaaatcccct cctcatggag
    70501 ataaataggc ttactacacc tattatattg tatcatattt tataaaaata tactattgct
    70561 tatttttaat cccatcccaa aactctttat tctccaaacc cagtcgccag atggcaattc
    70621 cctccaaccc cttttttttg gccaattgca ttttatactg gctgctttgc aaattttcaa
    70681 accagacaat tctctctttt ccactttcat ctttatagcg gtaataagga accttaaaag
    70741 tgtcattcca taagattttc ccaccatatt ttttcagata atcattaatt tcagagactg
    70801 taacagcctt tccatttttc ccttcaatgg gccagtcata accgtaagct gcaattccca
    70861 tcaatatttt tttgggtgaa atttctccta cggcataatt gataacgttt tctacccagt
    70921 cgtaagaagc tacaggaccc gggcttcctc ctgaccaatg ctcatcataa gtcatgatga
    70981 taaccctgtc tgcatattga cctatagttt tataatcgaa agcccctgtc cagttatcct
    71041 ttggatgatc agccattttt gcaggcacac atactgaaag tatatatttt tttggtccta
    71101 attcagcctt taattctttt ataaaagaac taaattccgc tcttacatct ggtcctatcc
    71161 cttcaatgtc tatggtcaca cctttgtagc cggacttttc tatttctgtc aggatatttt
    71221 ttacaagctg atttctaagg ataggatctt ccagaacttc ttcagccaat tctttatgaa
    71281 agctacctcc cttagcattg tgaataagca tagaagaatc tatctttttt tcacgggaat
    71341 aagctaatat ttctccttga tcataatcgg attctaagct tccatccgtt ttaatgccat
    71401 gactaaaagt cactacatcc gttatttttt ggtgattgct ttttaaagaa ttataagagc
    71461 ttaaatgacc ttcataatat ttgacggtaa aaccaagaac cgtatcaaat tgataagcta
    71521 taggctccat ttcttctaac ggacttcccg gatcttcaat aattatagaa ggcggaacga
    71581 ctcttaaaag ttgtcctacg taaataacat ctgatttgag ttgattccat tcttttagct
    71641 cttcaacagt tattttatgc tttatagcaa tcccataaag ggtatcccct ttttgtacca
    71701 cataatcaga aactttgggt aaactgctat tgattaaaac cactttagct tcatgattcc
    71761 aatccacagg aattttaaaa gcctctgaaa caaaacgaat aggaaccatc aacctgccat
    71821 ttataatttt gggtgctgca tctagaaaat gtgtcttgcc atctataaaa gctttaaggg
    71881 aattggcttg taaacggatt gttgtatctc cttttttaat gattgcaact ttttctttag
    71941 aatcccagta tacataagct cccaagcttt ctcccacaat tctgacagga accaggaccc
    72001 tcccgttttc tatgacagga ggcgtatcag attggacata acgattatta atacgaagtt
    72061 gaatggattt tgtctgatta aaagaagaac ttgcagcatt tacttttgtc gttggattat
    72121 gtagcatcaa agtagctgta ataatagaac cagccataaa cttgacggat tgaatcttta
    72181 cagcaggata agccgtttta atataactta atatattgtt tttttctttt tctaaagaca
    72241 attctccaaa ttcctttgca aactcagcat cctcaggtaa ataaataatt aaatgatatt
    72301 caccattttc ataaattaat ctttgtgcat aagaagccca attcatactt ctccttcttt
    72361 ccataataaa agccacttga taaagaggct ttaaatagtt ctattattat tatggaaaaa
    72421 atttttaaat ttatccctta taatcgttta attttctcgg cccgtctaag atttccctgg
    72481 ttataaataa agtcccgtca tccctggttt tgacatgcca tttaattaac ataatatagc
    72541 cgtcgataat ttcaattcct gtgatacagc ggggatgaac gcaactccca tcattaaagt
    72601 aaggggattc gcttaattcc gggaatgaag gcttgtgatt atgccctgct ataagaatat
    72661 gtttttcttt ttcaacccat tttgtcagcc tttttctaac ggctattttt ttataataat
    72721 tttttgctgt cctggtagga ttccttattc caaacacatt taaaggtctc cagaaatacc
    72781 tgactaaaaa tctggccaaa aaccacaact ggtcattaag caaatccccc tgatggccat
    72841 ggagtaaaaa aatctttttt cctgttattt tatgttttaa aatcagtcct tcatgaaact
    72901 ttatattttt aaacaaagga acatgtcttt tttgggcttc atcataatat tcccataatt
    72961 ttttttctac aaacttttct gagcgtttta caatatcgtg attgccataa atacaatata
    73021 gcctgccttc cctgtaaaac ttcgataaaa gccaaaagac atggctgtgg gtttgaacaa
    73081 tttcagtcat atcactgttt tcccacagct catctccgtc tcctaattca atataagtat
    73141 atcccttgtt aaaatagtaa gataaagcat aaaaatatat atgttgattt ctggcaaaat
    73201 catctgccca gctaccatca cccctatggc aatcactcat aataataatc tttgaactgt
    73261 catcaaaaag aatttcttct gcggtctcaa acaccctgga tatccgcttt aatgtattca
    73321 ttttcccacc attaaaaata atcctattcc atttatattc taaagcctct tttaaaagaa
    73381 cagataagta atcttttaat cttgattgtc atttagattt tttattgcta taaccaattt
    73441 attctttccc tagtttcaac aagacctttt aaaacttttt attaatatta atatccttgg
    73501 aggggttttt tatggccatt gatgatacaa gtataattga ttggattgca atgagccagg
    73561 atgagcaaaa cttagttatg ctaattaccg atcatctgga tagggatgaa gattttgaac
    73621 aaactcattt atttttgcta caggaaaaaa ttaatgtata tttagccttt attgacagtc
    73681 gtgaatatat agaagtctat ccaggcaaaa catttaatca tttcgttatt gaagttcatt
    73741 ttttaaatgc aatgagtcaa aactgtaaga atttcattga tactgtaaac agagaattag
    73801 aaaaatttaa tatccgaatc gaagccttta attaattcgt aaaaataaaa gctattgatg
    73861 aaaaatattc atcaatagct tttttctttc aaagctaaag attaaaaatt tacttagatt
    73921 tctatataat aattgacatt ataataaaaa tattagaaca ttataattaa ataatcattt
    73981 gacaggagcg ttttcaatga aaataataaa agagaatgag caactcattt tacaatccag
    74041 cttaatcaat aaacttataa attccctggt tgtccttggc atatttttat ttgtatcatt
    74101 tatgatcttt aagaatatgc attcttttga tctccttgtc ttaatatttc ctgcgattta
    74161 tcttatatta tttttgtttg tattttttaa aaaagattta tatcaatgca tttttgataa
    74221 agaccataaa agtataacaa tcatctggcg caacttcttc ttttctcata aaaaatattt
    74281 ttcttttaat gatttaattg cactgcaaat cgataaaaga tccgatgatg attacaatga
    74341 ctatattctt tacattgttt tttctgaaca gaaaattttt gttcattctg ccaggcaaag
    74401 aagtatcgtt gaagatatgg ccaatgcaat cattaacttt gttgacaatc ctgctcttaa
    74461 gattaatcca gccaccatta tttatgaaag tcataccagt aatacaaata acatgcctgc
    74521 cttttataaa tactattcaa aaggcagaga aggaaaattt accggcagcc tgtttgtcaa
    74581 aaaagtttcc ctgaaacgga cagataataa attaatctta aaaatcagaa aatctttcct
    74641 gtccgtactt gcttttttac ttcttctttt tttctttttc atttttacaa aagatatctt
    74701 aacaattgaa agtattactt tcaataaaat caataataac aaaataacct gcagtatcaa
    74761 aaactattta ttgggtaatg aaaatatgga aataagctct cttcatttag agcattttaa
    74821 agacgtccgt ttagatgttg atgacgaagg cggttcaatt tttataattc tttacgacaa
    74881 gaatcttgaa tacagcagca taaattttga tcaaaaagct tttgaacaag ttaaaaattt
    74941 tattgaagac aaagaagaaa cagaattaat tctttgggaa agttttagtt tttttgatct
    75001 cttttggttc ttactgctag ccttcttaat catcgcatac atgtttatac cctatatttc
    75061 ttgtattatc attgataaag acaactttat cattcaaatt aaatataaag gattcagaat
    75121 gaacaaacac gaaaagcatt caatccatgt tattaacagc ataagttata atgaatcagg
    75181 aaagcaatat tatttagaaa tagagttatt tggaaaaaaa atcgaattag gtgttttgac
    75241 tgataaagaa aaaatcagtg aaatcattga ctttttgaat tcctagtgcc tatccttcaa
    75301 taatttttat aaatactttt ctggttctcg gaccatcaaa ttcacaaaaa tatacactct
    75361 gccaggttcc taatacaggt ttgccttcat gaataataat ggtcttttct gctccgacat
    75421 aagaagattt aagatgagca tgggaattcc cttcaaaatg tctgtaatct cctttaaccg
    75481 gaaatacctt atcaagggtt accaggatat ctcttacaac atccggatca gcattttcat
    75541 taattgtaat gcctgctgtt gtatgaggac aataaaccac agctataccc tctttaattc
    75601 cagttttttc aatggcttgt tttagtaaat gagtaatatc taaaaactcc tgactacttt
    75661 ttgtagaaag taaaaattga aataaactac tcatcatcct acctctttct atattccttc
    75721 atatttttgc ttttatttag attatatcaa taaaccatcg attcgcaaca aaaatgagca
    75781 tatattatat atgctcaccg gaaaacactc attatcctaa aagttctcca agataaagat
    75841 tttctagacg gacattcatt tctccaatct gggcttttcc aaattcacca tgacagtctg
    75901 agcctgctgt aatcagcagg tttttttctt tacaaatctc taagcataac tttgtaattt
    75961 cctcagtatg ggtcggataa taacactcta taccatccag accaaaagcc agatatcttt
    76021 caattttctt tttaaaaaaa ggcatattcc tgtctttaat ggtttctcca ggatgggcta
    76081 aaactgcttt tcccccagcc tgatgaatgt aatcacaaac ttctttaaca gagggaaatt
    76141 ccacacaatc atatgaacaa ttataccgag gataaagttc aaaaccttcc ctgaggcttt
    76201 cagtaattcc tttctctata aaataatgga gtgctttcca tccgcctttt gtacggtcat
    76261 agttaaaaga cagaaagtcc tctaaagaaa tattatcaaa atccttttcc atttcactga
    76321 ttagtttaac actaatttca tccagcaaaa aacggtttct ttgaacaaat tggcaaaagg
    76381 ctttatcatt aaaattcatt ccatatccaa gaatatgaat atcctcgccc tcatcaagag
    76441 aatctaactc aacagccgaa agaaatttta tatcatatcc ctgacaaagg gcttgcagtt
    76501 cttttgaacc ttccagcaca ttatggtcag caattgccaa caatcctaaa ccttttttta
    76561 aagcaaccga taaaattttt tttggagaca tcgttccatc tgaataatag gaatgaatat
    76621 gtaaatctgc aattctgtct ttcattacct acctcctttg ataaaaacag gcagtaagac
    76681 caatgatgtc ttctgcctgg ttctttaatc attataattc ccttttttca aaaaataatt
    76741 tattctaatt cctctaactc ctctaactcc tctttaaagg cgtcgcagcc agaacagatt
    76801 cctacaaccc ttccatcatt aatagatgct tcattactac aaagaccaaa atcccagaaa
    76861 taaaacatgc cctttaatct taaaaagtaa gcacaattac tgcatcgatt gataggtact
    76921 tgattaacaa ctttactcca acgcttaaaa cagcgttctg aatgtttatc taagagctct
    76981 tcttccctgt taagttcttt ttcagtaata ttagcaggca tttcagtcca gatatttccg
    77041 gattcaaatg caaaacatct attattagga gaataaatac taataaccgt atctcttaaa
    77101 gattctgcca gaaattgaat ctcacagcca ttacttaaac ttaaggatat atcataagga
    77161 ctcgtttgct tgaaatcaat aatgacggaa gccactaatt catttaaaac aggtataata
    77221 tcttctgctt catcatattg accacatatg attgtagaat cctttattat cctccatgcg
    77281 catgattctg aaactaattt ccaaccttta tcgtctttat tctcatcgtc ccaattattt
    77341 aattctttat tttttaaatc taaagtaata ttattatcta aattaattct ggcatttata
    77401 cattctaaac caattaataa ctcaagcaac tctggcatat caaaacacac aatagttcct
    77461 cccttcctaa aactcataac ttatcacaca tcatgctgca acatattctt acaaaatatg
    77521 tagaattata tcatatatct tcataattat agaagataaa tatcaaaaca aatcaataaa
    77581 taaaagctaa catactaata atactataat ataaaaatat agtcaatttt tgaaattaaa
    77641 taatctttat tttgctcttc ttgcaaaata aagatttgca aaagattatt tttaattaat
    77701 ctctttaaca acctgacata aatatttctt tactgacaga taattttctt tgacttctga
    77761 taaacttaaa tcattggatg ctactatctt ttctcctata ttctcaagtg caataatggt
    77821 ctgaaataag agattatagg cttcatccat cttttcaaag gcttctttta aaacgccaag
    77881 ataaaaatct ttgagactaa tatcataact tttgcttaat gcataaataa taactgttgg
    77941 atcaatgact tttctatgaa ataaacagat cagaccttct attaaattct ccttattata
    78001 attcttctca ttaatacaat cattttcttc gaatgtactg acattacaag tacatttttc
    78061 aagtattaaa gcagaataaa tgactgcttt ttgtattatt tctttatctt tttcattttc
    78121 taatatatct aatagcttgt cttggctgat gtccatcata atttctccta atttagcaat
    78181 cattcgatac tataatgtta taattttgaa ttttttattt aataatacta tatcgtttaa
    78241 ttatcgtcaa ttatcaaagc ataaattttc ttttatggaa ttaaggatat atttttaaca
    78301 aaaataggac taaaagcaga caggcggctc aacataataa aatagtacta tttacatata
    78361 tcaataaaag attgtatact gtatttaata atgaaaataa aaataatagt ttgattatta
    78421 acaaacattt ttatacagct tctttttata ttctttttgc tttatcactt taaattaaaa
    78481 aaataaaagg aacggtattt accgctcctt tcaatgatta tagtaattat ttaaattctt
    78541 taaatacttc ttttttagct ccgcagattg gacatacttc tggcgcctca tccaaaactg
    78601 tataaccaca aacagggcat atgtaaacag cttcaagatc ataatctttc cctgctttgg
    78661 ccgcttcttg tgcttcttta aacatcttag catgaatttt ttcagcctcc agtgcataat
    78721 gaaaagaacg ttttgcatcc ttttcatttt gaaattctgc agtatttaag tatacgggat
    78781 acatttgttc cacttcatgg agttctccgt caatggcacc ttgaagattt tcaaccgttg
    78841 tggtattacc aaaaacgcct cctgccggaa cagtataatc tcctttttgt gcttttaata
    78901 cattaaagtg attattggca tgaacatatt ctgcatgggc aatggcctca aaaagaatac
    78961 caatgttgtt aaatccttct ttcctggcaa tttcacccca aataagatat cgcatatggg
    79021 ccatgctttc accaccatag gcagaacgta aaaaatcagc agtcattgca ttgttgacca
    79081 tgttatacct ccttaattat acaatatatg gcttatgata tgcaaattat gcttttttat
    79141 accttcatga agctattaaa tattcatttt atcatcatag gatttgtcaa attatgtaga
    79201 aaatatttat taattatttt ataatgtata tacaaaatgt tttgtttatg cggcatccta
    79261 ttgaaatgga tggggggata attcgatgcg tttagttcca gtgagttgct taaaaggtgg
    79321 ggaaattatc gcaaaaaccg ttatagacga aaatggttcc agattactcg caaaaaacac
    79381 aagttttaaa aagtactaca aaaagaaatt agaggccaga ggaattgaca ggatatatat
    79441 tgctgataaa ttatctgaag gtattgaaat taaggatgtt atttcagaaa agaccagaat
    79501 aaaaactata aatattgtaa aagacacctt agctattttt aaagcaaccc agcaaatcga
    79561 cgtctctcaa cttgaaactt cggtgatcca aattgtagaa gatatcctca gcaataaaga
    79621 aattttatac gatatggtcg atataaaaac aaaagatgat tatacctacg gacactcagt
    79681 caatgtcgct gttttatgtg ttatgctggg aagaaagatt ggcggagata aatcctattt
    79741 aaaaaaactg gccctgggag gtttactcca tgattttgga aaaatattag ttcctgatga
    79801 aatattaaat aagcccgata aactcacaga agaagaattt gatattatta agcagcatcc
    79861 tttccaaggc tatcaaattt ttaaagataa ccctttgtta acaccaatta gtaaagtgat
    79921 gattctaatg caccatgaaa aaataaatgg cgatggctat cccttaggaa tgacaaaaaa
    79981 ccagattcat gagggggcaa gaatctgttc tgtctgtgat gtatttgatg ccatgaccag
    80041 taaaagagcc tatcggaatg aatatacgat tgtaaatacc ttgaatgaaa tggaaagaat
    80101 gacaaagaac cacctggatg aagatttatt taaaaccttt aggggaatga tctgttatta
    80161 tcccgttgga acaacggtat tattaagtaa tgaaaccgtt gcaattgtag aacaaaataa
    80221 aattggttca atcgataagc caactgtaag agtcatttat gatttaaaaa caaaacaaga
    80281 agtctttaga agagtagatt tatctgctac aaaagaagta aaaattgaaa gagaattgat
    80341 ttctgaagaa tatattaata atcctgctat tttaagggcc atgcgcccat aaaaaattaa
    80401 aagggcagct ttaagctgtc cttttttaat agtagggata aggataagaa tattgataag
    80461 ggtattgata atatggatat tgataagatg gatatccgta aaaaggtatt ctcctgcgat
    80521 agcgtcttct tcttccaaaa agctctgtca gtaagagagc ccctgccagg gaacgaacca
    80581 gtctgtatct tggaattctc tgtctttcca tttctccttc atcaagaaga tccggcattt
    80641 cttgtataat cctttcataa actctgtcaa tcatttgttc tataatttcc tgatctggat
    80701 attcattgta gatagggctt ccgtcataat ccattttatc acattcatat tctatatgtt
    80761 tttgaattct ttgatttaat tcaggataca ttcttttcat atattccata tctctttcgt
    80821 caatatcatc ataatacatc ataccatttg gataaactcc cattggatac atattagatg
    80881 gataaggctt attattcatc attggttgct gtctgaaaga ttcttctatc cctttataat
    80941 tttcattatt ggcttcatat tcttttgaca tttatacttc ctccttatac atgattttat
    81001 atccaataac aatatatgca tgagctagta attggttctt gcctaaaaaa aaatccctga
    81061 aaaatcaggg attttattcg tcatggcgaa ttttcacaga tttaagtttc catatctcag
    81121 tggcatattc tttaatggtc ctgtcactgg aaaacttacc acttaaagca acatttacaa
    81181 tttgcatttt aacccatctt tctctgtcta tataagcttc caccattttt ttatgggtct
    81241 ttgcataact ttcaaaatct ttgagaacaa agtaaggatc accgccgttt aataagcttt
    81301 cataaatatc tctgaacagc tctgtatttt ccgggcataa aaacccattg attaattggg
    81361 ttaaaaccaa acggacatct tgattcatat tatagatatc ccaaggattg tatccgccat
    81421 ttttataata attcatgact tcttctgccg ttaaaccaaa gataaagata ttgtcgtctc
    81481 ctacctcttc tttgatttca atattggcac catctaaagt tccaatggtc actgccccgt
    81541 ttaacataaa tttcatatta ccagtaccag aagcttcttt gcccgccgtt gaaatttgct
    81601 gactgatgtc cgcagcaggg aataattttt cagctgcaga tacattatag ttttcaataa
    81661 atacaacctt aatctttcca tctatagaag catcgttgtt aataacatct gccacagaat
    81721 taataagctt tataatcagc ttagccattt tataagcaga agctgctttt gcgccaaaaa
    81781 taaaagtatg aggatgaaaa tccatacttg gattggcttt caattgatta tacaagtaca
    81841 tcacatgaag gatgtttaaa agctgtcttt tatattcatg aagtctttta atctgaatat
    81901 caaaaattga attaggatcc acttcaatat tgttgtgtgc tttaatatat tctgccagct
    81961 taactttatt ttcatattta atttccataa aacgctttct gaaggcttca tcttctttat
    82021 aaggcagaag ctttctaagc tccgttaagt ccgtaatcca gccgtctcca atcgtatcag
    82081 taatgaattg ggcaagattt gggtttgcct ttaacaacca tcttctttga gtaatcccat
    82141 tggttttgct gttaaatctt tcaggatata tttcgtaaaa atctttaaga acttcatgtt
    82201 ttaatatttc tgtatgcaaa gctgctaccc cgtttaccga atgactgccg acaatcgcaa
    82261 gataagccat tctcacctga ccatctgcaa taatagccat tcgccttatc ttttcactat
    82321 caaatttata ttgattaatt aactcataac aaaatctgcg gtttatttct tctacaattt
    82381 gataaattct cggaagcaga cggctgaata attcaatagg ccattgctcc agcgcttcag
    82441 acataatggt gtggttggta taggaacaga ctttttttgt aatatcccat gcttcatccc
    82501 aaggcaattc ttcttcatct accagaattc gcatcagttc tgccacagcc aaagaaggat
    82561 gggtatcgtt catttgaaag gcaactttat caggcagttc tttaaaatct gtatgatttg
    82621 ccttaaatct ttttatcgcc tgctgtatgc tggcagatat aaagaaatac tgttgcttta
    82681 accttaattc cttacccttt atatgattgt cactgggata taatactttt acaatgattt
    82741 gggctaaatt ttgttcttct aatgcctttt catattcacc ctggtcaaat ttctttaaat
    82801 caaaacgatt aactgctctg gcatcccaaa gtcttaaagt atttacggtc caattattat
    82861 aacctaaaat gggaatatca tagggaaccg caataaccga ctgataattt tcctgaataa
    82921 atttgtattc tccgttttcc tttttttcaa cccttacatt accaccaaat ttaacttcgc
    82981 aagcatattc aggtctgaat atttcccatg gattgccgta ttccagccaa ttatccggat
    83041 attccacctg ataaccgtct attatttttt gctcaaaaat gccatgtctg tatcggatgc
    83101 cacagccata agccgggtaa cctaaggtag acaaagaatc taaaaaacat gcagccagtc
    83161 ttcccaagcc accattacca agtcccgggt caggttcttc ttcttcaatt tcattataat
    83221 taatccccaa ttctgccaaa gcttcttcca cttctttttt aaccgccaga ttaatcagat
    83281 tgttccctaa agcccggccc gttaaaaatt ccatggataa ataataaacg atttttacat
    83341 cttctttttt atagacattg tctgtctcaa tccacctgtc ggcaatcaag tcttttaaaa
    83401 caaaagcaac tgcctggtaa atctcttgct ttgatgcttt atcgatggtc tttttagtca
    83461 atgttttcac ggtttcctgt acttcttcct taaattcttc tttatcaaag aaaaacttca
    83521 ttgaattccc cccatttctt aatggcttgt ttttaaacca tcatctatct tttaaagaga
    83581 ataatacttt tcaaaggcat taatgatata tttatggtct aaaacgcctc ctaattccct
    83641 tatcgaatgc attgaaagaa ttggattgcc tacatctata caactgatac ctaattgtct
    83701 tgccataatc ggccctatgg ttgttccacc ttttatatct gaacggttga caaaatattg
    83761 aacaggcacg ccggcttcct tacaaatttg ttcaaataca gccgttgtac tcccgtcact
    83821 ggcatattcc ttgttcgcac taagcttgat aacaggacca ccgtttatta aaggcctgtt
    83881 agtaggatct tgtttttctt cataattggg atgaaaggca tgagccatat ccgcagaaat
    83941 cataaaggaa ctggcaaggg ccctgaaata atcttccctg ctcttaccta aagcaagaag
    84001 aattctttca agaatatagg aagcagtcgg ggaatctgcc ccttctttgc ttgaactgcc
    84061 aacttcttca ttatcaaagc aaaagatgat attaattcct cttttggctt ttgtattcat
    84121 catggcctta agacctgcat gcaccatggc caaatcgtcc aatttaggtg cagaaataaa
    84181 ctccttatga atacctgcca gggaagcttt ttcaacagga taaagaaaca aatcaaaatc
    84241 caatatttta tcagtatcta tattcaaatg atcggctata aaccttatca ttttatgatt
    84301 ttcttccagc tcatctttaa tcatacctaa aagcggcaag gtatcttttt gcttattaaa
    84361 ttctatgcct tgattcacat ttctgttcat atgaatcgca aggcttggaa taattgcaac
    84421 gggctcttta aaatcaacca gtattttttc aggcttaaaa gggctgtccc ctttcaacag
    84481 aactctgcct gcaagagata acggtctgtc caaccagcta tataaaattg gtccgccata
    84541 ggattctgta tttaatttca tataacggtt acttttaatt tccggtgcag gctttatcct
    84601 aaaacctgga ctatctgtat gactgccaat aatcttaaaa ccttcttctt caatatttcc
    84661 ttctccgata tcaaaagcaa aaatagcaga atcgttaatg attgtataat atttgttgtt
    84721 tttttctaag atccattttt ctcttaaatc cagagctgta aaacctctat cttttaaagc
    84781 catttcaagt tgtttaacaa cgtgaaatgg tgaaggactg tcattaataa atgaaatcag
    84841 ttctttagct aatgtattat ccatatatat cccctttata tgattttagg ggagccttaa
    84901 aggcctcccc tattattata gcataaagtt ttttatttgt ttgtttaatt cttaagtttt
    84961 gggaaataag gactataggt cctatattct ttgaatggtc aactcaatag gttctccatt
    85021 aatcttccat tcttttgaat aaccttcttt gctgctgtct tcagcgatat ggtctcccag
    85081 aacttcagca gcaatttctt ctttatttct ttcgataatt tgagctaatt tttcattacc
    85141 ggaatagaaa actttaatat gatcttgtac ttcaaaagca gcctcttttc tcatagtctg
    85201 aatcttgctg atgatttctc ttacaaaacc ttcttcaatt aattcttccg taagattagt
    85261 atctaaaaca acggttaaat ctctttcagt ttctgcaata aagccttccg tttgtttggt
    85321 ttcaatcagc aaatcttctt caaaaagttc taccttttcc ccgtcaaatt caaagcttaa
    85381 aggattaccc tttctaagtt catccatcgc tttattgcca tcgatatttg ctaaagcttc
    85441 acggattttt cctaccaact taccatattt ggggcctaca gtttttagct gaggcttaaa
    85501 ggaataactt gtaaagtctg ctacatcatc cgtaagaata acttctttga tatttaattc
    85561 ttcagcaata atatctttaa agagctcagg caattctttt cctgtcttaa catacatctt
    85621 gcttaaaggt tgcctgttct taatattggc tgtatttctg catgctctgc ctaacacaac
    85681 aacattaagc acctgatcca tattgtcttc aagtacctta tcaatatatt tatcttctgc
    85741 ctgtggataa taacataaat gtacactttc aggagctgtt ttatccacat taatcacaag
    85801 atttctgtag atttcttcag ccataaatgg tgtaaatggc gcaattaatt ttactaaatc
    85861 cacaagaact gtataaaggg tcataaaggc attaatttta tcctgtggca tgtctttata
    85921 ccaaaaacgt tctctgcttc ttcttacata ccagttactt aaatcatcta caaaatcttg
    85981 cagtgccctt gaagcttcaa aaattctata attatccaga tgattgtcta ccgttttaat
    86041 taaagaatga agttttgata atatccactt atccattaag gataatttat catactctaa
    86101 ttggtattct ttcggattaa atttgtcaat gttggcataa agcacataaa aggcataggt
    86161 attccaaagc gtacccatga attttctttg tgcttcactc acagcttcac cataaaatct
    86221 gcttggtaac catggtgcac ttgtcgtata gaaataccat ctgaccgcat cggcaccttg
    86281 tttatctaaa acagtccagg gatctacaac atttccctta tgtttactca tctttaagcc
    86341 atctttatct tgtaccaacc ctaaaacaat aacattttca taagctggtc tgtcaaataa
    86401 aagggttgaa atagcatgta aggtatagaa ccatcctctt gtctggtcta cggcttcact
    86461 gatgaaatta gccgggaagt tttcttcaaa aatctctttg ttttcaaaag gatagtgcca
    86521 ttgtgcaaaa ggcatggaac cagaatcgta ccagcagtca atcacttctg atacccgttt
    86581 catactgccg ccgcactcag gacaagtaag atatacatta tcaatataag gcttatgcag
    86641 ttcaatatca tcaggtacgt tttgtcccat ttcttttaat tctgcaatgc tgccaatcac
    86701 atgacggtaa ccacaactac attcccagat tggaagaggg gttccccaat atctttcacg
    86761 gcttaagccc cagtcaataa cattttcaag gaagttcccg aaacggccgt cccggatatg
    86821 ttcaggatgc cagttaatct tttgattatt attaatgagt cggtctctta cttctgtcat
    86881 cttaataaac caggtatctc ttgcataata caaaagcggt gtatcgcatc tccagcagaa
    86941 tggataggag tgttcataat ctattgcttt ataaagcaga ttttttccat ctaaatgctt
    87001 aataatttca ggatctgcat cctttacaaa aacacctttc catggtgtaa cggcatcaac
    87061 aaattttcct tgttcattga ccaattgaac aaaaggaaga tcataaatac ggcctacttt
    87121 tgcgtcatct tcaccaaatg caggcgcagc atgaacgata cctgtaccat cggttaaagt
    87181 aacaaagtta tccgatacca catagtatgc ttttttatca ggttttgcaa aatcaaacag
    87241 aggttcatat tccgttccaa ctaaatcctg cccaatcatt gtgtccaaaa cttcatattc
    87301 ttcatttaaa accgtgcctg ctaaagcttc tgccaaaata taaacttcat cgccgcattt
    87361 tgccttaata tatttttcat ttggattaac cactaaagcg acgttagaag gtaaggtcca
    87421 gggagtggtt gtccatgcca tcaaataaac ttgcttttca ccttttacag caaacttaac
    87481 ataaattgaa gcctctttta cgtccttata accttgagcc acttcatggc tggataaaga
    87541 agttccacaa cgaggacaat aaggtacaat tttgtgacct ttatataaaa gtcccatatc
    87601 ccagattttt ttcaaagacc accaaacgga ctcaatatac tcgttgtgat aagtcacata
    87661 agggtttttc atatcggccc aatagcctac cctgtcgctc attttttccc attcactttg
    87721 atatttccaa acactttcct tacatttttt aataaagggc tctacgccgt aattttcaat
    87781 ctcaggttta ccgctaatac ccagcatttt ttccacttcc agttccacag gaagcccatg
    87841 ggtatcccag cctgcttttc taagcacctt ataccctttc atcgttttat atcttggaat
    87901 aatatctttt ataacccggg taaggatatg accaatatga ggtttaccat ttgccgtagg
    87961 aggcccgtca taaaaagtaa atgtaggaca accttctctt tctgaaatac ttttttcaaa
    88021 aatattattt tccttccaaa acttgaggat ttctttttcc cgttcaacaa aattaagatc
    88081 tgttgagact ttgttataca cagtataacc tccattctct tctcttttat tcccttctat
    88141 aaacaaaaaa ccttccatcc tttaacagga cgaaagggtt cctttcgtta taccacctga
    88201 tataatcaaa aacatgcata cctaatgata tgccttcctt ttaacggagg aaatccgtca
    88261 gagcctacta ttatttcggt ctgccactcc aaggtgattt tccgtattcc cttcatcatg
    88321 ggctcccacc atccccattt cgctgcagac tagagaaata cgtactcgtc cttttccttg
    88381 tgtttattcc tttaactcta ccacaactca atataatatt ataaaaagat tttcctgatt
    88441 tgtaaaggct tttatctaat ttatataaat ttatctatag aaaatactct atttttttta
    88501 tttttttgga ataatgaatt cactaatata tgctaaattt ctttgataaa aaaccgatat
    88561 aggtgtatac ttattctaaa ataactatgt aaatgaaagg aagtctaaaa tgagcggact
    88621 taatttaact ctccttacag acttgtacca actgacaatg atgcagggat acagggaggc
    88681 aaatgtaaaa aataatgtcg ttgtttttga tttgttttac cggacaaatc catctaattc
    88741 cggctttgcc attgctgcag gacttcaaca ggtcattgaa tatattgaaa acctgtcttt
    88801 ttccaatgaa gatattgact atctgagaac gaccaattta tttaatgaag actttttaca
    88861 agacttaaaa aattttagat ttacaggaga catttacgct gttcctgaag gaacggttgt
    88921 atttccaaaa gagcctttac ttaggataaa agcacccatt tttgaagccc aatttattga
    88981 aacagctctt ttaaacatcg ttaatcatca aagccttatt gcaacaaaag cagcaagagt
    89041 atgctgggca gctgaagggg ataccgtact ggaatttggc ttaagacgtg ctcaggggcc
    89101 tgatgcagga atttatggtg cacgagctgc tataattgga ggctgtgctt ctacttccaa
    89161 cgttttagca ggtcagctgt ttaatgtccc aattagcggc acccatgccc acagctgggt
    89221 tatgagtttc cctgatgaac tgacggcttt cagggaatat gcaaaggtat tccctaacac
    89281 ctgtatcttg ctcgtagata cttacgatac tcttaaatct ggcgttccta atgccattaa
    89341 agtttttaaa gaattaaaag aaaaaaatat caaaccaaag ctcatgggca tccgcttaga
    89401 tagcggagat ttggcttatt tgtctaaaaa agccagagaa atgttagatc aggcaggttt
    89461 tcccgaagca gttatcagtg cttccagcga tctggatgag tatcttatcg ctgatttaaa
    89521 acgacaaggt gctaaaatta atgtctgggg tgttggcaca cgcttaatta cttctaaaga
    89581 ctgtcctgct tttggaggcg tttataaatt atctgctgaa gcagataaag acggtaactt
    89641 tattccaaaa attaagttat ctgaaaatcc cgataaagta accaatcctg gagttaaaaa
    89701 aatcatccgg gtatatgata aaaccacagg taaaattaaa gcagacttaa ttgctttaga
    89761 ggatgaaagt ttcagcgaaa aggaaccttt aagattattt gatcccgtga acacatggaa
    89821 acaaatgaaa ctaaaacctg gtgaatttag cttaagagaa ttactggttc cagtttttat
    89881 taatggaaaa tgtgtttatc agtctccttc ggttatggaa atacgaagct actgtcaaca
    89941 agaactgtct accctctggg atgaatgtag gagattgacc aatcctcaca ttctgccagt
    90001 agacttgtcg caaaaattat atgacttaaa gaaaaaaatg attgatgaga ttaggcaaag
    90061 caatgccgat taattttaat aaaagataaa ataaaagtgt gcaagcacac ttttatttta
    90121 tctttatctg ttattttgat tcatcgtatt tcctcttgtc gtattggttg tcgttcttgt
    90181 attcacatta tttctattat ttacatttgt attatttgta ttattccttg tatttgtaat
    90241 tctattattc atatctgttc catttaaagg attgggtaaa gttcttgttg gtacctgagt
    90301 tgtaatacct cttcttatat tttcttctct ggcatttatc ccatcaggtg ttaaatccaa
    90361 atcatcatta gcgtcataac cataatcgtc attataatca taaccccaat cattatctat
    90421 catatcgttt ccattatcca gtattccatc gtcatcacgg atatttcttg ctgttctgtt
    90481 attggtgcag gctgaaacaa gtacaagtac ggataaaaga ccaatgatga atagaaatcc
    90541 tctgtttttc attaaaatac ctccttttat aatttcaata ttattgtttc tctattttaa
    90601 agaaaaatgt tgtaattttt ttggcaaaga tgcaaaatag ttatgatata ataaatgtat
    90661 atcactgcat ttttaaaaca ggaggaaatt atgcgaactt taatttgtat tctatttata
    90721 ctgataagcg ggataatatc tcttttattt tcaataaaat atgcattttt aaaaaaattt
    90781 tctacacaag aaaaacaaat ccaatatgct tataaaagtc ttcaaagact tacaaaaggt
    90841 ctgctttttt gcgcaggtac ccgattaaat gtaaaaggga aagaaaatat tccaaaaaat
    90901 caaacacttt tgtttatttg taatcataaa agtctctttg atatcccaat cttagtgagc
    90961 tctattgata ttcctatggc ttttgttggt aaaatacaac tgaaaaaagt acctatcatc
    91021 tcattttgga tgaaaaaaat caattgtatt tttatggaca gagatgatat ccgccagtct
    91081 ttaaaagcaa ttaatcaggg tatcgaacag cttaaaaacg gacaatccct attaatattc
    91141 ccggaaggaa caagaattaa aggggaagaa ttaggagaat ttaaaaaggg cagcttaaag
    91201 cttgccaaca aaagcaatgt acccattatt cctatttata taaaaaatgc tgataaaata
    91261 ttggaaaatc agtttccatg gataaaagca acccatattg acgttcgtat tggcaaacca
    91321 attattatag aaaaattaga taccaacagt cagaagaatc tctcagaata tgttaaaaag
    91381 caaattgaaa tattaaaagc agatatttaa agcggaggct tttttgcctc cgctttttat
    91441 atactgttaa ttttataaaa ttggtgataa tagtcttgaa atggattctt taaattttat
    91501 accaaaactt cttttctcat aaatctccct ggtaatttcc ttactatcag ccagatcttt
    91561 cataaattgc tcttgcagtt gattgttgat aatccggtca tatataaatg catttacttc
    91621 aaaattcatc ttaaaacttc tgatatcgag atttgctgtt ccaacggtac tgatgacatc
    91681 atcaactaaa atcattttac tgtggagaaa gcctttttca taagtgtaac atttaacccc
    91741 ggcctgcagt aattctccaa tataggataa ggaagcccaa taaacaaaca tatgatctgg
    91801 cttattaggg ataataatcc gcacatctac tccggataaa gcggccagtt ttaatgcttc
    91861 taataagctg tcgtcaggaa taaaatatgg tgtatgtata tagattcttt tctttgctaa
    91921 agtgatcatt tttaaataac cgtttctaat ggaactccat tttgaatccg gaccgctgga
    91981 tacaatttgt attcccgtat aacctaaacc ttctttagga ggaaaatatt tatcttttat
    92041 caaatacggt tccttcgtag agaatcgcca atccaataaa aatctaagct ccaaataatc
    92101 aactgctcca cctttaattc ttaaatgagt atctctccaa taacccattt ttttggataa
    92161 tccaagatat tcttttccta tattaatacc accaacatag gctgtttgtc catcaataac
    92221 gcatattttt ctatgattcc tataattgat acggacatta atataaggta aaaagggtgg
    92281 gaaaaagcaa ctaatctttc caccagcctc aagcaatggc tgaaaaaatt tgtctgaaag
    92341 cctgatacaa cccatgccat cataaagaag ccttacttca accccttcct tggctttaag
    92401 ggttaaaagt tctataatct gtttacctag ccaatcgttt cggataatat aatactccat
    92461 atgaataaac tttttagctt tttttatgtc ctcaagcatt ttgtcgaatt tttcattacc
    92521 atcattaaaa atttctactg tattatcatg agtaaaaaga gcattatggg caaccaaatg
    92581 aaggtaaatt aaatccttac tgtttttaat actgggataa gccaattcta tatctttccg
    92641 gcttaatatt ttttcttgct gatgaatcat tttttcaagt ctgtcgtctg cttctttggc
    92701 agcaaatatt tttttctttc ttaaatcctg acctaaaaac aaatataaga aaaaacctaa
    92761 tacaggaaca aaaaacaaaa tcatcagcca ggcccaggtt gttgaaggat ttcttctttc
    92821 aaaaaagacc agtaaaacag caaacagaat attaacaatt aaaagtaaaa ataaaagcca
    92881 gttaaccact tccaaaacaa tcctcctttc aattgatcat tgcaaaaaac acagaatata
    92941 ttattttaac aaattcctat ttcatatttt taaattcaat gatacaatta taggggaatt
    93001 atatctcaat tattactacc caagtagatt agaaacaaag gaggacaacc agtgcaagtt
    93061 tggcaaatta ttttaattat tcttgcggtt atagtgctta ttcttactgt tttatatttt
    93121 gtaggcaaaa gaatgcaaac taaaatggac cagcagcaaa gcttaataaa ccaacacaaa
    93181 atgactacat ctatccttgt tattgataag aaaaaagaac gaattattaa ttctaacctt
    93241 ccaaaagccg tcattgatca atttccaaaa gtgttccgct ataaaaaaat gccacttgtt
    93301 aaagcaaaaa taggtccaca aattactacc ttaatctgtg ataacaaagt attcaaagac
    93361 ctgccaacta aaaaagtcgt taaagtagaa attgccggac tttatattct tggtatcaaa
    93421 tcagtaaaaa aataagcttt tcaaaagccc tccttctgga agggcttttt aattactttc
    93481 ataatctaca acaacacgag aattaggaac aacaatttct ttcataaact caaccacttt
    93541 aagatgttca ggatgaacct gataagtttg caggtcttca tccgttttaa attctgaata
    93601 taggattaag tcataagccg cttcagtggt attataatta attcctgctt ctaaaaaaac
    93661 aatttcacta atcttgcttt ttaaagcttc cagtttttct ttagcttgtc ttaaagcttc
    93721 ctctttgctg ccatattttg tttcgtcttt aacacgccac attacaatat gttttatcat
    93781 ttgctttcct cccagtaaaa ttttgattat aattaaaata tctgtttaaa tatatatata
    93841 ataatataaa tataaaagct tatccagcct tttgatattt atttatgggt aattattgga
    93901 ggtcaatatg aaaaactgga atatcattgc cattattcac gaaatatact gtcgctttca
    93961 agatgatgaa gtccctgctt taagtgcaca gttgactttt tatcttactt tatctttttt
    94021 tccatttttg atatttgtcc tgacgatcct aaattataca cctattagtt ctgaaatatt
    94081 tctatcacta ctatccaatt tacttccaaa agaaacctat tatttagtat atcatactat
    94141 taaagaaact gtaagtacca gcaacagaac cctattatct tttggaatgg ttacaacttt
    94201 atggagtgct tcaaacggcg ttaattccgt tataagaggt ttgaataaag catacgatga
    94261 agaagaaaca cgctcttttt tcagactgag aggaatttct ttaattttta caattgtttt
    94321 atctataacc atcctcttgt ctttggcact aattgtcttt ggcaggtata ttggcatttt
    94381 tatggttact catttaggct ttaaccactc agtcagcagc gtatggaata cgttcaggca
    94441 aatcattatg attactacca tctttgttgt attgctcttt ttatataagt atatgcctaa
    94501 ccggaaactt ttgtacaggg atgtattgcc tggcactatt ttttcaactt tcttctggtt
    94561 tgtgctttcc aacctttttt ctaagtacat tgaaaacttc ggaaaatata atattatgta
    94621 tggaagtatt gctggtatta ttattttact tctatggata tatatgagca gcattgttct
    94681 tcttttaggc ggagaaatta atggcgttat cctttttgca aaaaatggga aagtgaaacc
    94741 cagagctaaa aaatttggct tccctatccc cttttgcaaa aaaaagaaaa atcattctta
    94801 aattaaaggt ataattgctt aatccgcttc tgtttttata gccttttcta ctctcactga
    94861 ttgtatctgc ttctttttca agtaaatata acctactgtg actacgacta aagaacctat
    94921 cgcatcaaca atgagatcct ccatcgtatc tgccagggct ttttgccctg tcagtaccgt
    94981 cccttcttca gtaataaact tctgcatatt agttcctaat atacagtcgg cagcaaattc
    95041 atatatttcc caaatagtcc cacaagctaa agcaaaacaa aaagcaaaca aggctacaaa
    95101 aaaaggactt agatgaacaa aaggtctttc catattattc agataactaa caataaaaaa
    95161 tcccatagca cctaacatgg ctgcactaaa gccatgcaaa atcaaatccc agtaaggaat
    95221 tttaaaatag aagttcctga cttccccaag atagatggca caaaataaaa atataaaata
    95281 tattatttcc attagatcgg gtatttctat acgatatctt ctctcaatct gtgaaggtaa
    95341 aaacatcact ataatcccga ccagacattg aagtatcatc agtacatagt cactttttat
    95401 tctgtcatac ggcttatcca aagcttccat tgcaggtgct gtaaaaagca tataaaccgc
    95461 ataaaaaagc gatatcacca gtaaagctaa aacaatttta aaaataaaat tctttcttct
    95521 ttcatacttt ttaaattctg gtttcaattt aatccccttt actattttat tagtataact
    95581 atatttatca ttaatattta ttatgattaa aaaccgtatt cttttctgtc aagcggcagc
    95641 tgtttaacca aatgcgttta aaaaagaccc caaatcttag ttcatatttt ttatattaga
    95701 atatgtaatt atattataac atatttttct cctaatagat tcagagggcc ctttcaagtt
    95761 agtaaaataa atatatggaa taattaaatg aaaccagatt aacccaaaaa caagtataaa
    95821 tatgactttc attataatct tttcaataat tctgtttttt tcgcctggaa ttcctcttct
    95881 gtcaaaatcc cactattttt aagagcggct aatttttcta tttgatctaa tacattttct
    95941 tttggctgga ctactttttc ctgaataatc accttttctt ctttgttccc ttttctctta
    96001 tactcattta ctattttaat aattctttct gcttccagtc ttaaaagtgt ctcaatcctg
    96061 aagcttgttc cagcaacata tatttccaga tcacattgct taaatgcagt agacagcatg
    96121 tccgttatca aacctccatt attggattta gcagtagaaa ctgaaacaat atctttatat
    96181 tcaaagcgtt ttatttgttc tgatataccc aaaacttccc tgttaataat cattaattgt
    96241 tgattcgtac aaataacata atccctggct gataaagaat caagtcccca tgcaacatag
    96301 agtaaatatt catcttcata aagaaagccc tttatcctgt tatataattt tttgtctaaa
    96361 cgcttttcta ttgcttttga tatttcctta tcctctatct ttaaatgacc agttgttaac
    96421 cggtcaatat atcctttaaa attcgctaaa tcatcggaat gatatttgat atccattaca
    96481 aaaagatcca cggaattgat ttgaacagtc attttatctg catcttgttt gacttttaat
    96541 tcttgaattt ttttaccatc aatttttcct ttaaagagat tatctgtttc cggatctttc
    96601 atagaaaaat ataagttttt attcgtaata agaaaacctt cgttcttgct caattcatat
    96661 gcgactaaaa ctgtttcatt atcttttaaa cttttagcat atgtatcttt tgcattatct
    96721 aatgtttttt cagaaatata agaataaaat tcaaagttac tgttgctgta tttttctcct
    96781 attgtacata atttttcata atattcctgt gtaaacctaa gcagctgatt gttatcaaat
    96841 ctatttaaag agttgcttta ttgtctatta aattttcttc tatactttta atttttttac
    96901 taaatatcat ttgagaaact ctattttgct cttctctttc taaccttttc ttttctgttt
    96961 gcttatccac caattctata cctttattta taatctgttc tactttttgc tgcctttctt
    97021 ctttaacttc tttataaagc cctttaaatt cctctttctt aacaatcatt ttttcatcta
    97081 tagattttaa taaggcatta taaatgttct tataatttct ttccattgta cctgctgtat
    97141 ccgttggaac ggttatatct acaactactt tcgtctcagt ttccccttca tcaaaaaaag
    97201 ttattttaat ttttcctctt cctccatctg tccctgtcat cttgaaaaac tttccttgta
    97261 tctcataaat atctttattg acctgcaggt ctttaaaata attctctaat gctaaattat
    97321 gagaaagtat ctcaatcgct tctgctttcc tccgattcat tgtaaaagac tctctttgtg
    97381 tctttgacat attacccgtt atatcttctg cttttttctt aatattttct gtagccgttt
    97441 ttgctttttc cattatgttc gcttcatcta atgctttttt tacatcatct gaaattttct
    97501 taaagttatc aaataagctc atcttctctc tccttaaatt gattattaaa attttgtaaa
    97561 attaacatca ataaattaaa gatttgaaaa cagctctttt attacacctc cttctttttc
    97621 aaagaataaa ttgcttattt ctccataagc attgaaataa tacaattatt atactatatt
    97681 gatgagctct cttctaccaa tttatggttt attttgagtt ttctcgacaa gaatcgataa
    97741 gctataaaag aaaaagtcca gaaaagctaa aatctcttct ggactaatta tttatgatat
    97801 ggttcacctt ttatgatttt aaaccaacga tatacctgct ccaataaaat cagcctcatc
    97861 agctgatgcg gaaaagtcat ttttgaaaag ctaagctgaa aatcagcccg gttcagaact
    97921 tcttttgaga gtccaatgga gcctcctatg ataaaagtga tatgacttct tccttttaaa
    97981 cttaaatctt ctattttttt agccatttct tctgaagaaa agactttgcc ttctatagct
    98041 aaggcaatta cgtaggtatc ttcttttata tgatctaaaa tcctttttcc ttctttgttt
    98101 tttacctctt cttcctgagc caggctcata ttttcagggg ctttttcatc agccagttcg
    98161 ataatttcca gtttacaata ccttgataag cgttttgcat attcggctat gcccatatga
    98221 aaatattttt ctttaagttt tcctacggca ataatcgtta ttttcatatg atctccttat
    98281 acaattaaaa ggcaagccaa aagactgccc ccttaatgtc ctttattatt tttttactct
    98341 tataatttca gcacccatgg catttaactt cttttcaatg gcttcgtaac ctctgtcaat
    98401 atattgaaga ttatttatag tggtttttcc ttctgctgcc aaagcagcta acacaagtcc
    98461 tgcacctgcg cgaaggtctg tcgcagaaac ctgtgcacct gaaagctttt tcacaccttt
    98521 tataacggct actctgcctt ctactttaat atctgccccc aggcgtttaa gttcatctat
    98581 gtactgaaaa cggttttccc aaacactttc tgtgataata ctggtcccct ctgcaatact
    98641 taaaagagct gacatctgag gttgtaaatc ggttgggaaa ccaggataag gcagggtttt
    98701 aatattgact gccttaagtc tgtcttttgc tttaaccctt atgtaatcat cgccttcttc
    98761 caactcaaca cccatttcaa gaagtttagc tgaaacagat tccatatgct taggaatcaa
    98821 attgtgaacc gttacatcgc ctctggtaat tgcaccagct atcatataag tacctgcttc
    98881 aatctgatct ggtataatca tgtaattggt gccagataac ttcttaacac ctacaatacg
    98941 gataacatcc gtacctgcac ctttaatgtt agctcccatg ctgtttaaga agttggctgt
    99001 atccaccacg tgaggttctt tagccgcatt ttctatggtt gtcgtcccct cagctaaaac
    99061 ggctgccaac ataatattaa ttgttgcacc tacactgacc acgtccagat aaatttgggt
    99121 acctactaat ttgtctgcac gcgcttttat cataccgtgt tctacaacaa cctctgcacc
    99181 taaagccgta aagcctttaa tatgttgatc tatcggtctg gttccaaaat tacatcctcc
    99241 cggaaaggcc acttctgcct gtttatacct accgagaagg gctcctaaca ggtaatatga
    99301 agcccgaatt ttcttaacag cttcatacgt tgcacaaaaa ttattcaaat ttctgctgtc
    99361 aattctcaag gtatgagggt ctaaaaaatc gcattttaca cccatttcat ttaatgtatc
    99421 tgctaaactg atgacatcat cgatataagg taaattttca ataacacata catcattagc
    99481 aagtatagct gcaggaatca tagcaacagc ggcatttttg gcgccgctta caaatacttc
    99541 tccttcaagt ctttttccac cgtttacaat taattcttcc aatattaaca cctcttaaat
    99601 cggatttatc tactttgtaa aatcataatt atcaattggt tataaattaa gaaatagctc
    99661 tcagaaaaaa acaatagaaa cttttctata tacatactaa aattttcaaa ttctatacct
    99721 caattcatta taacattaaa atttaataga agaaagcccc tttattaaaa atattatcta
    99781 aaattgtatc tttgcatttt caaatgacat tttgctcgta tatgcattca gaaaaaagct
    99841 tcttccgtct gccagggtta tcctgtagta agggattgct tcccagccac tgtctgtcct
    99901 tttatccgat tctatgaggt agccaatgtc aattttttga atcacaataa tactcttgtt
    99961 atcttcaatc tttttaaatt gattcataaa accaaaaagt atttcatcgg gcgcataaat
   100021 gcttcttgaa aactcggtat attttacagg tttcatttta taaatatcag catactctat
   100081 tccttccgcc ctaataagta tctctatata actattgtat atataactgt ctttataagt
   100141 ttcataatac aaaaacctat ataccccttt ttcttcttcc ataaaatcta cttttaaatc
   100201 cgataaatta taatccattt tttttaaaaa attatcggct aattttttag catcttcttt
   100261 tgaatatatg acttgttcct taaaattaag ctcttcactg taactaattc ttccgtctga
   100321 aaatccaatc gtttttccat cctttttata gactgtctga ataatgtccg gctcaatttt
   100381 ttctatatat atactaattc catcatttgt attaaaaaaa gttttaataa tctgatcttt
   100441 ttcatcttct gaaaaaataa caggagcaag gattaatttt ctcatagggg gatattgctt
   100501 aggcagcaaa gtataaagca tgatattatt ttcatataat aactttttta tattactctc
   100561 ttgctggctg gttaaagtat aaatactgga gttcttttga taattcatat aagccaatac
   100621 gatatttaaa ataagaaaaa tgacaataag tatgtttttt accttcgccc aattcataca
   100681 gcattacccc tataataata aaataaaatt aatcttcttg cgttgcttta ataggaaaaa
   100741 tcatgtcttc tgtgtgtata atccaaaaaa tttccgggtc tttatctaaa gattcttgat
   100801 aataagccca atacatatca tgaatttttg cttcttcttc aacaaggaaa ttaaagcggt
   100861 taatggcttc gaggaaggat acatttaaat cgacctcttt atctaataaa taaccttccc
   100921 aggtcaatct tttatattgt ttaacttttt tattctctac gatgatctta acagcatgat
   100981 tcattcccgt ttcttctgct atttcccggg aaaaaatata aggcatatca ttaataatat
   101041 aatcaaaata gaattcccat cctttttccg tttccttttg ctctgacaag taaagaaaag
   101101 tttcaaggga agaattattg ctaaaggctt tttcatgctc atctaaaaaa tctttagcta
   101161 ttttgtaact ttccaaaaag cttgtttttg tactaacggt tgaactggtt tgactggtat
   101221 attccaaaac gccatttgga aagtatttaa cgattacatt tccttcaata taaaaaatcg
   101281 ttccatcact aatcttttgc tttctcttta agcttggatt aataaaaaga ggattgacca
   101341 gtttgtccaa cttttcctca ttgagcttac ctgcttcttt taatgaattt ttcattctga
   101401 ttaattgata gctggggaat tcattaaaag caggtaaaaa aacattgttt tcaaattgat
   101461 tcatcatgat ttgcttggtt gagttatata tcaggcctgt agattcttca ctaatcttgt
   101521 taatcaaatt atatatattc aattggtctt gtttgattgc cacgatatag actttattgc
   101581 ttttttcatt aataaaataa caattgattt cctgattaat atttttatct ggtataacca
   101641 aaagctgatt aaactcgcct atattagaaa tcgtactgtt ttcatttcca aagcctgaca
   101701 aggggatgga atctgtaaaa gcatataaaa tagacttttg agaaaaaatt tttctccagt
   101761 ccaaactttc ttcttttata aagcgtcctt caaaaagagc cctgactaaa atatctccca
   101821 tctcttcctg agcttcttta aattcttcac tggaccggtt aagaacattg taaaccccac
   101881 cttcaaatcc taaattgata ataattcttg caggttgcaa ggcagatatg ggctcagcag
   101941 aatattttat aatattcgat gtattttttg cccataaatt ataaaaaaag ttacggcttg
   102001 acaaattgcc aaaccataac tctcctgtct gaatacaact tattataatc aatgcaaata
   102061 atagtaatgt ttttaatctt gatttatcca tctaaatcac caaatgatta ttatctattt
   102121 taccagtgtc tctaaagcat ttttcccatc cacagctgtg tcaagaatat taataatttt
   102181 tatttcttct aattctttct tgatagtcgt atccttacaa tctatcctga atttaaaagc
   102241 tttttcctgt tcatattgct tagcaacaat aataatataa ttattacctt ctttaagttc
   102301 tattaattgc gaaaacagtc cagaggcccc aaccacttgc gtataaattt ttgcattatc
   102361 ttttttacct tcctgatata caacaaaggt tataaccatt cctttatctg cttcaccaga
   102421 tatatttcta tatctgtcaa aagttacttc atgttttttc gtaatacctg ccgtaatttt
   102481 tatcatatcc aaatccaaaa taccagattt ttctatatta acagaactta ctggatcgtt
   102541 ttctaatgaa tttgctctta cttcggcagt tgaaaaaaac atcaccaaag caaaaatccc
   102601 tatatataaa aacttacgca ttgggttacc tccttccata tgatatattt aaatcactgc
   102661 tattatatta tacaagatat atattacaaa aacattacat gatgcttaat tttaaattac
   102721 aagctttatt ttgggaattt catagtaatg gttgttccct taccatactc actttctgca
   102781 taaatctttc ctttatgatg ctccataatt tctttagcaa ttgccagacc cagaccggta
   102841 cctcccattt gtctggacct tgccttgtct accctgtaga aacgttcaaa aatccgaggt
   102901 aaatcacttt ccgggatacc aaatcctgta tctgtaactt ttacgacaac aaaatcctcc
   102961 tcttctgaca gacttacatt aatctttgct tcttcaggac tatattttat ggcattactt
   103021 atcacattgc ttaaaacttg ctgaattctt tccggatcaa tatttataat gattttttca
   103081 ttcgttatgg aataaccaag gttctgattt ttcttttgtg caagaatttg atgctgctct
   103141 acacttttaa taatcagttg ggcgatatta acggacttta aattaaattt gacttgcttg
   103201 ttatctaacc tggataactc cagtaaatct tgtaccaatg cagtcatcct gtctgcttca
   103261 ttattaataa cctggaggaa agaaagcgct aaatctttat tatccactgc accatccaac
   103321 aaagtttctg cataactttt tactgtcgtt aagggcgttc ttaattcatg agaaacgttt
   103381 gccacaaact cttttctcat ttcttctaat tctttttgtt ctgttacgtc ctgcaataca
   103441 ataataaccc cgtcactttc accctttttc cttccataag tcgcaaaaca gccatttata
   103501 tatttatctt ttacaagcat caaatgctgt tttatatacc ctggcttcat cgtaagaaac
   103561 tcatcaaagc ttaactcaat accatttctc ttaaagatat cataaaaata ctgttctttg
   103621 tccgtcatat ccagcattgt atgtgcagca ggattggcat ggatcaaatg accttttctg
   103681 tcaaatgcta aaataccgtc tgccatatga gtaataaccg tctctaattt attcttttca
   103741 cccgatatgg ttaataacat ttgatttaat tcttcagcca tatgattaaa gctttcagtt
   103801 aactgaccta tttcatcgtt agactttaca tcaattttag tgtctaaatg accttctgcc
   103861 atttcttttg cttttctggt caatattttt atgggttcgg ttaaagtctt ggcaaagtat
   103921 gtgccaaaaa tgactgtaat aaagagcgct aaaaaggatg caataacaat aatatttgta
   103981 atgattgcaa tgctattaga ggtctcctga gtagaagcgc ggataaaaat gatatttttc
   104041 agtacatctc caactaatat gggataagca taatcaacat attcttgaac taagcctccc
   104101 tctaaaattt gaaccggatc tcctactgca ggttttttac taataatcgt ttcatagaca
   104161 actcttttat tccattgctc ctgtccattt tcttcagtat ctggaatgat aatgtttccc
   104221 tggtcatcta atatgtatat tttcttatta ataaaaatac ttctgtcgcc aacccatgta
   104281 ttgatatctt tccacctggg attaatagag ccgtcatccg atacaatatt ttccctaatg
   104341 gattctgctg tattttttaa ttcattatgt atttttttat attcattatc tcttatttgc
   104401 cagataataa aggttccact ggaaatcatt acaatgagaa ctaaggttaa gtatatgaat
   104461 actaacctcc actgtatgct tttcatacca ttacccctta aaataatagc ctacccctcg
   104521 ttttgttaaa atatattctg cacgactggg atcatcttca attttttctc ttaagcgtct
   104581 tacggttaca tctactgtcc ggatatcacc ataatattca taaccccaca ctttttctaa
   104641 caatacctct cttgaaaaga cctgaccttt ttgtctggcc aaaaacttca ataattcaaa
   104701 ttccctcagg gttaattcga tgatttttcc gtccttcgtc acttcatatt tttcagtatc
   104761 aatctttaaa ttattatgga caatgacatt tttatttttt tcttctttta tttctttttc
   104821 aacaacggca atccgtctta aattagcttt tactcttgcc attaattctc ttacactaaa
   104881 aggtttggtg acataatcat ctgcacctaa ttccagacct aaaactttat caacttcttc
   104941 tgctctggct gttaacatta caataggtgt ctcttttttt tctcttattt ttctacatac
   105001 ctgaaagccg tctattttgg gcatcattat atccagaaga attaagtccg gatcttctga
   105061 aaagactaaa tccagcgctt cttccccatc ataagctatc aatacttcat agccttcttt
   105121 ttctaaatta taagcaataa tatctgcaat acttttttcg tcatctacca ctaaaatttt
   105181 ttgtgccact taaatcacca tcccattaga tccatgatat atacattatt aaaatgcata
   105241 tttctatctt gatgctaatt ttattgtatc aaaaataata aattgtgtaa aatagattat
   105301 tataaaaggc gaacattctg atatgttcgc cctgaatctt tttatttcaa ataattataa
   105361 ggattttttg cttctccatt tagtcttatt tcaaagtgca ggtgtgaacc tgtactgttg
   105421 ccggtacttc ccatggctgc aatctgctca cccttggcaa ccttttgacc tacctgaacg
   105481 taaattttac ttaaatgagc ataatagctt tcaaatccat tgccatgatt aattcttaca
   105541 agatatccgt atcctccatg ccagcctgct tcaaccactg tacctccatc agctgcatat
   105601 acaggcgtac caattggtgc agcaagatct attcctgcat ggaatctgcc ccaacggtaa
   105661 ccaaaagttg aagttaatgt tcctgaagta ggcatcctga aagaacctgt tgcacttttt
   105721 ggtggtggtg tttttgttcc tacaacgact acggattctt tgggttcttt tatgactttt
   105781 tcggatataa tttctgtacc ttcttcatag ccatttatct taatcttata agcagttatt
   105841 tctttttctc cttcttcacc tggctcaatc actttaatgt aagtcttgta ttgtgtgtca
   105901 tcttctacat atctgacagg tttttcaata ggctcagtat aagtaaattc ttcttttgtc
   105961 ataaccgaaa gtataggctt aggtatattc aggttaatct cctgaccaat ttgtaatatt
   106021 ccctcttctt ctaatccagg attggctttt agtatatctt ctattgaaag accgtatctt
   106081 tctgaaattc cccacagggt gtctccttct tcgatggtat aaattctttg cgcctcagtg
   106141 ctctttgtta agagttcaac tgcatcatta accgcaatca tctcatctga aaccttgata
   106201 cacttgggct ctactttaac ctcttcaacg aaagaaataa gttcaggctt tatgccttct
   106261 tcaatatatt tagcctttat tttttcaagc acttcttctg cttccttgtt attaatcagt
   106321 gtagcctgtt ctacaccatc gactttaata acataagctt ttatatcata agaaagcgaa
   106381 ttctttaatt tagtcagtaa ttcgtctttt gaaataatct ctttttggga agcacggaca
   106441 ggttctacaa ataattcact gcaaacctca atgctgcctc ctaaatcttt ttcgattgaa
   106501 agcttaactt cgtttaataa attatctgct gtttcctctt caagaataat acccgccact
   106561 tctccatcca gcataacaga aaaagcattt ggagtttgag aaatttcaaa tcccagataa
   106621 aaaactgctg taagtacaat aatcaataca aaaagcctga aatactttat catatcaata
   106681 gattggattc tttccaaatt aatcatattt ttgtcctcca taaaaattat tatggctttt
   106741 taataagtat gtaacacatt tgtaaaaaat acaatcttat tttaacaaat agataatgtt
   106801 ttgtaaaggc atatataaaa aaaatagccc caaaattttg gggctaataa ggtgacaaaa
   106861 attatcccat tacaacaaca acttcatgtt ctttattgtc agataaagga ggaattatgt
   106921 tggaatctaa ttcttttccg tctaaaataa ctttttttac accctttgat acatgatctg
   106981 ggttttctat cttgatttta tatcttgtac ctctgaattc tcttgttacg gtataaccat
   107041 cccatgcttt aggaatacat ggatcaactt ttaatccgtc ataatccggc ttaattccta
   107101 aaatccattg ggaaatggtt acaaagttcc atgcagcagt tcctgtaagc catgaattct
   107161 ttgcctgtcc ttgattgggg gcatctttac ccgcaatcat ttgtgcataa acataaggtt
   107221 ctaatttatg aatttcactg atttcttcta agtaagccgg tgctattctc ttatagattt
   107281 caaaggcacg gtctccacgg ccaataacgg tttcagcaca tgcaatccat ggattgttat
   107341 ggcagaaaat acctgcattt tccttatatc caggaggata gctggtgatt tctcctaatt
   107401 tataatcata agttgtgtaa gccggttgtt gcaatacaat tccataaggt gtttctaatc
   107461 tttcttttac agaatctaat gctttctgag ctaaaccttc ttctacgcca attccagcca
   107521 ttacacaaaa gccttgtggc tcaataaaga tttggccttc tttattttcc ttactgccta
   107581 ctttacctcc aaaagcatcg taagcacgaa ggaaccattc tccatcgtaa ccatattcaa
   107641 tggttgcctt aatcatatca tcaatgtgct tttgagcttt tttggcttct tcttctaaac
   107701 ctcttctctt acaaagttca acatattctt taccgataaa tacaaacata ccagctataa
   107761 atacagattc agccactctt ccatttggat ccccataagt ttggaaggat tctcctggtt
   107821 ctgtagagaa gcagttaaga ttcagacagt cattccagtc tgctcttccg attaaaggaa
   107881 gtccatgagg tcctaagtta ttcaccacat gatagaaaga tgctgttaag tgatcaaaca
   107941 gggaacctgt tttatcagga ttacaatcaa aaggtacttc ttcatctaaa atagcaaagt
   108001 cccctgtttc ctttatgtac gcactgactg ccaggattaa ccacaagggg tcatcattga
   108061 agcctccgcc aatatcatga tttccttttt tggttaaagg ctgatattga tggtatgcac
   108121 ttccatcctc aaattgtgta gcagccaaat caagaattct ttctctcgcc ctgtcaggaa
   108181 tctgatgaac aaaacctaaa agatcctggt ttgaatctct gaagcccata cctcttccaa
   108241 ttcctgattc gaagtaagaa gcacttctgg acatattaaa ggtaatcata cattgatatg
   108301 gattccagat atttaccatg cggtttagct tttcatcatg gctgtccaag atatattttg
   108361 aaagaagtcc atcccaatat acttttaaat cttccaatgc tttttctacg ctggcattgt
   108421 ctttaaattg ctcttgcatt gcataagctt tttctttatt gattacattc agggcaatcc
   108481 atttattatc tttgtcattt tcaacatatc caagaacaaa tacaaatacc ttctcttctc
   108541 cagctttaag ggtgaagttg atattatgag aaccaacagg tgaccatcca ctggcaactg
   108601 aattattgga tttaccttgt gtaactactt gtggcgcatc caaattatta tatagaccaa
   108661 ggaaactgtc cctgtcggta tcaaaaccat tgatttcagt atttacccag taaaaggcgt
   108721 aatggtttct tctttccctg tattcagtct tgtgataaat agcactgcct tctatttcca
   108781 cttctcctgt acttaaattt ctttggaaat tggtcatatc atcataagca tcccatagac
   108841 aaaactctac aaaagaaaac agagataatt gtttatcggc atttgaatta ttttttactt
   108901 ttactctatg aacttctcca ttgtatccca atggaacaaa ataagttaca ttaactctga
   108961 catcatttct ttcaccggca atggatgtat agcctaaacc atgtctgcat tcatagaagt
   109021 ctaaatcttt ttttacaggc atccagccag gagtccagaa gtctccgtta tcaaaaagat
   109081 aataataacg gcctccatta tcaatgggaa tattgttgta gcggtatctt gtaatccttc
   109141 ttaatctggc atcacggtaa aaacaatatc cccctcctgt gttagaaata agaccaaaga
   109201 aatctttgtt ccccagataa ttaatccagg gatatggggt ttttggagtc gtgataacat
   109261 actccctgtt tttatcgtca aaatagccga atttcatatg agccctcctt aaaaaatata
   109321 ttttaatatt ttactttcta ctttattagt gcataaatat cgcatgaaca cgctttcatt
   109381 tttgcatata aagctacacc taataaaata gaaaaactac gcttaaaaaa caagaatgat
   109441 tactaagatt tttaaatact aagccattta aacagctctc tatcttatag attactagta
   109501 aatctaaaaa taatcaagta aaaagaaata aaaattaaag attatttatt tttatctaaa
   109561 tttgctatca aataaacaaa aaagaggata ctggcatatt gttttccagt ctcctcttat
   109621 caatttatgc ctgcaacaag atcttccgtt actttatttt gataaaatcg ttattttttc
   109681 tgcctggtct cccccataag ttttcattgt aatgactacc agcatacctt ttttcaaatc
   109741 tcttctgctt aatcttctgt tgtctttaaa aatttctaca cccttaccca tacggattcg
   109801 ttttgttcct ataaaatctg attctgtcct gatatcaata taatcacctg aaaaacttat
   109861 attttctata taaccttgta cagtactcag ttttatttgc ttgctaagaa ctaattcttc
   109921 aacttccata ctgtctaaat ataatacagc ttcttgtccc aaacggatat catatatgga
   109981 gatttcttca tcggttaatc tgtcaacgat tttagcacca tacattaggg gataagtttt
   110041 tattgtacca tcttcatgct ctatggtcac cttagactga ttggctgaaa caaggacttc
   110101 ctgaataatt cctgttactc tggagctgta accctcagcc caaacatcaa ttactttttc
   110161 atattccagt cctaaggtaa ttttatcccc tattcttaaa tcattccagg ctgcctcatc
   110221 gactcctttt ttagctatgg ttacatcact gccaatacta tactctaccg tgtcattttt
   110281 tacttcaagc acaagtttat ataagccgtt cgttcgtttt ttctctatca aagtgcctgt
   110341 gacttccctt tggctttcaa taacttctcc cttagtcaga agactgcctt tcacctgagc
   110401 aacaataatg tctccctttt taatatctga taagcttaat tctttattgt tctttgtaat
   110461 aatggtatta ctattgacat tataagtttc tgattcttct ttgatttctc ctttatagtt
   110521 aacatatttt acgatgattt ctacgtcgga gtcttttaca ctatttataa ttccttctac
   110581 ttttcttgct gaaacattga gattatcctg ataatccgta taagaatgat ttgatgtact
   110641 gcctgtgttt gtataattac cagaagaatc cggagataca ggacttgtag tatctatcct
   110701 ggtaatctta acaacatagt caatgtttcc ttcattaacc agctgaatca ttacattgtc
   110761 acctttttta atactttctt tattggtttc ctttgttcca atataatatg tggcattttc
   110821 ttttatacga taacaagagg ttttatttcc tgaattaatc cccagataat gataatcact
   110881 gttaaaatat tctactttcc cctgcagata aacttcttta tccgttgaag ttgtttgatt
   110941 tgaacttgtc tgactgcctg tccctaaaac agattcaacc agtacctttg ccagtacggc
   111001 tttatacact ttacccttag gatcaaactt gacattttct cctccctgca aaacgcctgc
   111061 atcgttcaga taagcaatat gggcttttgc attttcagaa attaaagcat catcaagaaa
   111121 tctcgtcttc gtataattat taatcgcata ttcttctttt ccaattaatc ttacaataaa
   111181 aacagccata tcttccctgc tgagagaagc tacttgttct gtgccatcag cctgttttat
   111241 aataaactta ttcaaatctt ctgtttttaa aatgcccttt tgtaaaagat atgcaatttc
   111301 ttcattaaaa tttttgtaat aagcttcgtc ccatttctta aagtttttag tatattggtc
   111361 taaaacggat ttgtatttct gataagcttc atcagcgtat ttcttttcag cttcagtaat
   111421 attgatattt tctaaaggat ctttataacc tgcaattttg gctgcaatgc ctgaaaaata
   111481 aaaataatcg atgacttcct ggggtttaaa ttcattttgt aaatttcccg tcatccagtt
   111541 catttctgtg gccttttgaa cgacatcata agcccagtga ttttcaggca catctatgta
   111601 acttacttgt gcaaaaacag aagttgacat acaaaaagcc aggcttaaag aaatcactgc
   111661 tttacttatt attttcatat taatcctcct tgcgatggaa taaactgctc taaaaacctt
   111721 ataatctaac tattatttta agtcaaaaga agtgcatatg caagtatttc catataaatt
   111781 gtcccagggc ttttttctgt ccatttttaa gaaaaatcat taaaaaaaat atctgctcct
   111841 ttcaattgcc agttttttta tattgcctgc ctcttcgaaa ttggctacaa tagctttgta
   111901 aagttgattg acataagtcg ccaacataat ctcttctttt acatcttatt tttttatttg
   111961 tattgtgtat aagggcttac cccttgagat tgttcctgtc actgccaccc tcttttctgt
   112021 aaatacatga ttaatttgcc aaggaatatc acttgattat gttaaccatc tcacctataa
   112081 tattatattg ttggtagaca taagtcatca acataattct atgttttaac atagtaaata
   112141 aggaggaaag ctgaatgaag aatcttaaaa agaaactctt atccttagca ttagctgctg
   112201 ctgtggtatt atcttccaca aacttcgctt ttgcagggaa cttatccaat gccgataagt
   112261 tgaatatttt aacaaatctt ggcgttatca ctggtgaagg taacggcgtt atcggaaccc
   112321 agacaatgac aagatatcgt gcctttgtca tgcaacttaa attaatgggt aaatatgatg
   112381 aaatgaataa ttttgcatgg gaaggcaaag caacatttaa agatattaat tccagtcata
   112441 gtcaattcat tcgtaaattg gctgcgtact taaaagcaca ccctgaaatt ggtatttcag
   112501 gagatcacct tggaaatctt aatccaatgg ctcctatttc cggcagggag tacatgaaaa
   112561 tcatgttagt acgtcttggc tatgttgaaa atgttgactt tacctgggca agtattccaa
   112621 catttgcata cagcaaaggt ttaattgaca atccaactga agttgaagga tcaaatattc
   112681 aacttcttca aatcgccgac tttacttatg atgctttaat gtctaagcct gttggcagtg
   112741 aaaagacttt aggcgaacaa ttaggacttt ttaatttcga tattacattg gataaagtcg
   112801 atgcaacaac cgaaaatgaa accatcatta tttcaggttc tataaccaat gacggcactg
   112861 taaccgttaa cggaaaagct gccagtgtaa ataataagaa attttctgca gaagttgcat
   112921 taactttggg tgaaaaccaa attaccatta ctgctgttga ttctaacggc attaaagttg
   112981 aaaaaacaat taaagttgta agagaataca aagacttaaa ggtacttaaa gttgaaggtt
   113041 acaacttaaa acaatttgct attgaattct caaaagaatt ggataaagat tctgtaacca
   113101 acagccgctt aggtcttgga gcaaatgata tctttgaaat ttccaaagac ggtaaaacta
   113161 tattggtaac cttagataca ccattacgtc aaacaactga taccaagtat accatcaaag
   113221 atattaaaga cattactggc aaaaagattg ccaaaactga aattgttgta aatgtatttg
   113281 ataatgaact tcctgaagtt gaaaaagtta tcgctaaagg aaaccaatac attactgtga
   113341 aattcagcga accagttgaa aatgctgact tattaagctc atacagaatc gacgaatctt
   113401 tagttcgtgg ttccattgaa gctaacagta aaaaaactga gtttactatt aacttaaaaa
   113461 ctgcattagc agacggcgag catacccttt ctatcagcaa tgatattaaa gatgctgcag
   113521 gattcaacct taaagctgtg gatattgatt ttactgttgt aaaagatgaa gacgctccgg
   113581 aagctgaaat catcagtgca agccaaacca aagttgtcgt tgaattcaat gaagagatta
   113641 agggattaga taaatctcag gtttcatgga aatctggcag caaaaaaggt gttgcttcca
   113701 gcgtggtaca aagtgacaac gatgaattcg tatacgaaat cacttttgct gaaggcaatt
   113761 acgttccttt aaatggcgca acccttattc ttgaaaatgt taaagatttc tctgacaata
   113821 ttgctaagaa gattgaaatt aacttcgtac ctgaagttga ccttgaaaga cctgaggtta
   113881 ttgacgttga agcagatgaa aacaatcaaa acatccttta tgtaaccttc agtgaagacg
   113941 ttaataccaa tggtaaatat gtattaattg ataaaaataa caaagaaaag actgcaagtt
   114001 caatcgcata tgcagattct gatgcgaaga ataagattaa agttaccttt acaggcgttg
   114061 cagcaggtga ctatactctt gaagtaagtg aagtaacaga tctttctcca ttagccaatg
   114121 aacttctgcc tgttctttta gaaatcacca taaatgattt agaaagagtg tctattgact
   114181 cctggggcag aattgtatca ggcgatagca agagaattct tatcagattt actgaagaag
   114241 tagatgaaaa gaaggcaaca aacctcaaca attacaaatt ccgttttgta agcggtgcac
   114301 tttacagctt accagatgat actgaaatca acttattagg tgatgataaa accgttgaaa
   114361 ttatcttccc tgaagaatgg aaattaaacg gacgcactta tgacttagat gatttcgaaa
   114421 gcattaaata cattcaagtt gaaaaagtaa ccgatgtagc tggcaatgaa atgtatgctc
   114481 aattgctgga tattgatgct ggtactttaa cacttgattc tgaagcacca gtcgttgtat
   114541 ccgctgctgt taccggtgaa aatacaattg aacttgaatt aaataaccct attgatgaaa
   114601 aatctttaga cagaagagac ttcattgtta aattagtttc cggtttatca ttaaatgtat
   114661 atagagttga ttattctgat tcagatgatg aatataaatt aactttatat gtacgtgaaa
   114721 aattagacca aaatgctaaa tttgacggaa ctggcttaag attaaccttg gcagaaactg
   114781 tatccacaaa gaatatttat ggttctgccc tgtcaatggc ttcagctctt gcgatagaag
   114841 acaaatataa tcctgaagca actgtagcaa gtgctgtata tggaaacaaa actgaaattg
   114901 tatttgaagt tggcgaagct gtacaatttg gatttggcag tggcttagct ctggtaactt
   114961 ctgaaagcgt tgaagatggt gttacaactg taacacttga aagaggaagc gctgctaacg
   115021 acttgttaat cagccgattc acagttaaga aagatggtaa gaagttaaac atcgaaaaga
   115081 tccaaattgt tgacggtagc aaaattgtat ttgtaattaa cgatggtaac gaagattggg
   115141 atggagataa gttagaaata acttataacg gattagataa tcctgcatac tccattaccg
   115201 atgcaagcgg caacattctt gtgaactttg atgatgtaga agtatccatc gaaaacatta
   115261 actaataact gcaaaaagac tagggaattt tccctagtct ttttctgtct ttaaaagtct
   115321 tttctcagga acgctttgtt tatttaattg gcatagtccg gcatcaaagc aggattataa
   115381 aaagctgttt ttaattgatt gtgttgagcc attaattttt gtaattccaa ttctgcatta
   115441 tggattgcca gttccccttg ttttaatttc aaaggaataa tcattcccag cttatattgt
   115501 tgtattaata tacggtaaga ttcctttgcc ttttctatat tgagcttttg tgcttcaatt
   115561 tgtttctcaa gctgtaggat ttgattatac ctggattcaa gagatttttc aagattcttg
   115621 atcgttgcct cttcagagag ttctgcctgc ttatattcat tgtccttcat tgaataactc
   115681 tctccatttg gagtccaggt tgtactgtgg gattttcttt ctaagtcttt taactccttg
   115741 gcctttttac tgtaccatac ataaaaatct ttggaaatca tatcactgat atgacctttt
   115801 aagctaatgt cttcaagggg cttatatccg atatttttat tcaatatata acgcatattt
   115861 gttggttgtc cgatgagatt attaagttca gtatatttag agtccagaga aagctttaaa
   115921 gtctctaatt gcttttccaa ttgttcttgt tccatttctg cctgagaaag attgtatcgg
   115981 ctttctaatc cgagttcata tctttttctt attatatcaa ggttttccct ggataactcg
   116041 acagatgcct gtgtaatggc taattgttct tcaagttcaa caatttcata ataagctttt
   116101 tctgccataa actgcacaga ttcttttatc aattcttttt gcttctttgc agcttcagct
   116161 cccatgtccg cagtacttct tctcagtaaa gaaacaacgg catcttcctc aattgctaca
   116221 taacctccaa gaccatcaga aaccataggc gttcttgatg caatgacagc tgcttcttcg
   116281 gcccgctcag cagctaaatt ctgcaggcga agtgaaatat ttcccccgac agccattctc
   116341 ttaacatcct ctaaacttaa ttccctggca tttttatact caatggcttc tatcttaagc
   116401 tttgggatat tattttctac aagattatta tccattggaa cgctttcatc tcgcgttttt
   116461 tcgtctgaca gtttttcgcc tgaagggtct tcttctgccg gatttatatt ctcttttgtt
   116521 gttatatata tcgctgcttc atctttattc cattctacct gggcatccag tgcttcagca
   116581 atccatctta aagatacaaa agtcacgccg tctttaattt cgggagcaag gcttagctca
   116641 attttttctc cattcttata agcatctttt gttcctactt taagttcaat atagttttca
   116701 tctttttggg ctttgataat ttttttattt gcatcccatt ccacttctgc acccaggatt
   116761 tcaaaaagtt cccgcattcc tatcagacta gtgcctgatt ttacaatggg ctggttttta
   116821 aggagatatc tctcaccatt ccacaggagc gatacgccac tggtatcagc ccgaagactt
   116881 gaaggcatta aaaaagtaat gataagtccc gtaataagcc agttcttatg tcttctcacc
   116941 taatcccccc taatcatttt aattatcttt tgttgctttt tatatatacg atatacatga
   117001 attaaagtat gataaatttt accatgtttt tattttgcta gaataaaaac agacctcagt
   117061 tgaggtctgt ttttattaaa ataatatctt aatcttagtt aaatgttgct gtcttagttg
   117121 tgttatccca ttgtacaggt acgcctaagc ctttagcaat ccatcccatt ggaacgaaga
   117181 ttctgccgtc tttagcttca gctactgtat ccatggtaat ttcagcacca ttaactttca
   117241 taacgttgct tccaagtact agttgaacga tacggttacc attcataata gtaactgtct
   117301 tgttggagta cagaacgtca tctgggttaa ctcctaagcc ttgagcaacg tatcttacag
   117361 gaaccatgat acgtccgtct ttagcatatg gagctacgtc tgcttgtaat gttactccat
   117421 caactgtgta atttacagaa tcaagagtaa atgtagcagt gtatttagcg ttcttgcttt
   117481 gttcgatgtc ttcagtgttt ggagtagtaa ggtctacata taagtctttg taccagctgc
   117541 cccatccgtt ccacttgtca ccaagtgttc taacagccat atcgtaggtt ccttcaggaa
   117601 ttgtacggtc aactaaaagt tttgcacctt caagagtaat tgtggaagct tgtttacgtg
   117661 aagctttctt aacttcaact acaagtacac gtccgcttgt ttttgcaaat tcatattcgt
   117721 atattctact taatgttgaa gtttttgttc ttcttcttac atagtattta tctttatcaa
   117781 ttgttccatc agcagcacca caaataccta atacttcttc ccaagttgat gctgtagcac
   117841 ggaatttagt ataatcttta gtatttacat cgtattcgta tacttcataa gtgtatactc
   117901 ctattggaac tgtttcaaag tatttgtcaa ctgctttttc ttctaagatg ctagcttcta
   117961 cttcgatgtc gccatcaact tttacttctg gcttagtttc ccaagatacg taatcttcaa
   118021 gttcgaagta taattttgtt ccttttgtta atttaccttt atcagtttca gttactgtaa
   118081 ttgtaccagc gtcttgaact ttaagacctg ctttaagttc tttagcttca acgtctactg
   118141 tgaatggtac agtaacttca ccaagtacta attcttcttc atatcctaat aatgcattac
   118201 cttcgaatgt agctgtgata tctccggaac tagcagcttt agcttctaag gtaacttcaa
   118261 atactaattt tgttgtctta gttggatctt ctttcttaat gtttgcgaaa tcaatccaat
   118321 gatcatcaca ttccacatat gcatatggtg atttaactac atttttatca tcatcaacat
   118381 atgagtaatt tgttcttctt tcagcaatgt tttcgcagtc agcagcgata atttttacag
   118441 cgctcttgtc tgcgattctt acgtagtcag ggaatttaat tgttgttctt ctttcagaag
   118501 cccaagaacc ttgaattact tcttcaagtg taattttgta tgtttgtttg tcataacctg
   118561 ctactaaatc tttttcttct gcaacagaaa ctttaatacc aaagtcaaca tattctccta
   118621 ctttgagaac ttctttggtt acgccgcagc catcgtcatg gcttccgtcg atttcaactt
   118681 caacttcacc aaaatctgcg ttacgttttg caatgatttg taatccgcta atgtaaagtg
   118741 ttgctctttg attacgtcgt ccaactgcat tgattccttt aatatcaaga acaagaacag
   118801 agttggatga tttgtcaaca tcgattactg ctttcatttt agcatttgat aatgtgccgc
   118861 tacctgttac agtaacttct cttgctacgc caggattcca aatccaagca aaatcatcgt
   118921 taaggatttc taatcttaat tcaccgtctt taagtgtacc aatttttaat tcatcaattt
   118981 caagagtatt taaatctaaa acattgttaa agctcttaga atcaatttta gcttttgtag
   119041 cgtctttagc aaatactgca attacatatg tgcctttgct tacgccactg ttataatcat
   119101 caattgttaa ttctacatct gcttcttcag tgcattttac attatccaaa ggaatagcaa
   119161 attggaaatt acctggtgca ctggtctttc cataaacttt tctataaact tgaatctgca
   119221 ctttagtgtc tgtaagtttt ttcattgcga aaccatcgaa atcatcaaag gccacaattt
   119281 gataaacttg gtttgttacc ttttgaccat aaacttggtt tgttaccttt tgaccataaa
   119341 tgttatacaa tgttggttct tcagaagata cattttccca tataccgttt ttcaatatta
   119401 aattgaaata tgcacctggc tcaatttctt ccattaaata ttctttttct tcaatggtta
   119461 agtatgagcc acctacttcg ccaatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   119521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   119581 nnnnnncatt aaatattctt tttcttcaat ggttaagtat gagccaccta cttcgccaat
   119641 ggtctgatct ttagcaatta caggtaccct tgaaacgcga tttgtagaag ctgcgaagga
   119701 taccattggt aaagatgtga gaaccatagc gcctgctaat aaagcagcta actttcttct
   119761 catttttttt cctcccttaa ttttttttta gagatttggt aaatcattct aatctctagt
   119821 ttacagaaat gattatagca ccgcaaattt tgccagtcaa ctaaattaat agatatgtaa
   119881 taataatgta atattgtcca ttattcttgt aatattgtta aactggcaaa agatgctcgc
   119941 ggagattatt ataatatgga attattttct ttgcaagttt tttttgcttt gtaacagttc
   120001 cgtaacaatg caacaataaa aagaccgctg atacggtcct ttatcatgca agttcttatg
   120061 ttttcttacg ctaattcatt aattttttct attaaagttt ctttgctgac ggcattaggt
   120121 attttttcta ccacttctcc atttttaaag aaaataaaag tcggtacact cataattctg
   120181 tatttggctg ctaagtctct tgcctggtca acatctattt tatatacttt tgcttttcct
   120241 ttcatttctt ctgatacctg atcaacgatt ggcgccatca ttttacaggg tccgcaccag
   120301 gtagcaaaaa agtcgaccaa aaccaattct gaggcttcta atacttctgt ttgaaattct
   120361 tgagcattta attcttttgc catatgaatt ctcctttcat tcaggaatat taaatatttg
   120421 ttagtatcct ttattttgct gcagggccca gatgcaggtc tgcttgctta cagtataagt
   120481 atgcctgaaa atatttatat tataagtaaa gtatattgcc tgtttaatat ttatgcaaga
   120541 aattataatg gtttatttat tcttcatatg taataatttc aatcttttta atgatgacag
   120601 gatttttggg tatacctgaa ttggggtcag ccatctctac agcagcaatt ttatcgacta
   120661 cctccatgcc ctcaaaaacc tggccaaaaa ccgtatggtt aaagtccagc cagggtgtac
   120721 ctcctttttt tctgtataaa tggatgattt cttctggata acccgctaat ttcatctgtt
   120781 gcaaaagttc ttcttccact acaggctttt gaacaataaa gaactgactt ccattcgtat
   120841 tggggccgct atttgccatg gcaactgccc ctctgtaatg tagaacttct gtgctgaatt
   120901 catcttcaaa aggttgtccc cagatactct ctcctccagt tccatcacca ttgggatctc
   120961 ccccttgaat cataaaatca ttaataatcc tgtgaaatgt taagccatca taatagccat
   121021 tttttgcatg cgttttaaaa ttttctaccg ctttaggcgc atattcagga aaaaaacgaa
   121081 ttttaataac acccatatcc gttgttaaaa cagcaatttc ttctcctttt tcgggtagct
   121141 taaattgagg gctggctgct tcttcaccag aattacagcc tgttaaaatg aggcttagta
   121201 aagttaaaaa agataaaaat ctgattactt ttctcaatga atttcttcct ttctttctaa
   121261 aatcctccta tgctcaaaca ttataccaca gatcaagaaa agatatagcc attttaatga
   121321 attactttta tattttttca aaagacatat attctaaaaa ctccacaaaa gattttatac
   121381 cttcaggcgt ttgtttatag accccggcat cttccaaaac ttttttaaac ttcattccta
   121441 cttccttttg caagatattt cttccttctt ttacagtacc atatttaaat tgcatttctt
   121501 caatccaggc cttatgtttc accaattccg gcttcttttc catttgctta tgcacagata
   121561 tttctcctgt taatattttt tctattgctt ctaattccct gttaagccgt cctggcagta
   121621 tagccaggcc cataacttca atcaggccaa tattttcttt tttaatgtga tgaacatctg
   121681 gatgaggatg aaaaatcccg tcaggatatt gagggcttgt cctgttattt cttaaaacca
   121741 ggtcaagttc ccattggcct tttcttcttt tgcgcaaaat aggcgttatg gtattatgag
   121801 caattttttt atctttttcc agcgtaaaag caataatatc tgcctcttta tcaaagtagc
   121861 tcctccagct ttcaagaata aaaaaggcta actcttctaa tgctttcctg tccgctgctt
   121921 ttagtctgat aacagacata ggccagctaa gaatttcaca actgacgttc ttataatcag
   121981 ggtgtttaaa accatacaga acttttgctt tttctatagg gaaaatatac ctgccgcctt
   122041 gaaaatgatc atgacttaaa atggagccgc ctactatcgg caaatctgca ttggagccta
   122101 taaagtaatg gggaaatctt tcaataaaat caaacattct ctcaaaagtc ttcctggtta
   122161 gctgcatagg tacatgttct ctgttaaaaa caatgcaatg ttcattataa tagacataag
   122221 gagagtactg aaaataccat gcttcatcag aaagtttaag gggtatgaca cggtgatttt
   122281 gtcttgcggg atgattgatc cttccgggat atcctacatt ttccaggcaa agaagacact
   122341 tgggataatt ttcttgtgct gcattttttg ccgctgcaat ttcttttgga tccttttctg
   122401 gtttggataa attgatggta atctctaatt ccccgtattc cgtatgaact gtccaatata
   122461 aattttttaa aatacggttc atgcggatat aggttgtcgc cttggaaaag gcataaaaat
   122521 ctgctgtagc tttttctata ccttgttctc tttctaatct ttcaaacttt tttatcactt
   122581 cccctgccct tggtgtcagg atacccataa ttttcgtatc caataaatcc cggcaagtga
   122641 ttgagttttc ttttaaaatt cccttttcaa aggcatagtc caaaagctta tcgagtatat
   122701 cataaacatc ttcttttaaa tcttctttat ctatggcacc tttataaggt tctttaattt
   122761 ccagtaaatc catcagggca tttcttgtgg gaatcatgtc gtgctccgtg ataagtcctt
   122821 tatccagggc atattgtaaa agcttttcta tttctttttc aggctttaac ggcttcattg
   122881 atgactcctt tctaatccgc atatccattg ggatgagata aatgccagtt ccatgctgtt
   122941 tctataattt cttccaatga cttatattga gcttcccagc ctaattcctt cttaatttta
   123001 tcagaagaag ctacaagaac tgcaggatct cctgctcttc ttccgactat ttttacagga
   123061 atatcttttt tagttacttt ttttgctgct tctatggttt ctttaacact aaagccctgt
   123121 ccattaccca ggttgtatat ggcagaagga ccgtcctttt ttaatttttc cagagctaaa
   123181 atatgggcat gagctagatc tgttacatga atataatccc tgatgcaagt accatcatgg
   123241 gtattgtaat catcaccaaa tatcattatt tcttttcttt ttcctaaagc cacctgcaaa
   123301 ataagtggta ttaaatgtgt ttctggcctg tggtcttctc caattcttcc gttggcatgg
   123361 gctcctgccg cattgaaata gcgaagaact acatatttta taccataagc ctgatcacac
   123421 cattttaaca atttttcaac agccagctta ctttcaccat atggattcgt tggaaatgtt
   123481 ttatcatctt ccataagagg aatattttca ggctctccat aagttgctgc tgtggaagaa
   123541 aagacaatat tctttacttg atattctttc atttttttca aaaggcaaat catcccgtag
   123601 acattgttgt catagtaatc taaaggattg gttacacttt ctcctacaag agattctgca
   123661 gcaaaatcca taactgcatg aataggattt tctttaaaaa ctctatccat aaaatttagg
   123721 tctcttaaat cacctatata gagttttgcc ttgggattta cagctttctt gtgacctttt
   123781 tgaagattat ccacaacgac aacctcttca cctgttgcaa ttaattcttc tactgcatga
   123841 cttccaatat aaccagcgcc accacatact aatactgaca tcttaaccct ccagtcttat
   123901 ttttcttacg ccatctccaa ttccggcaat ataaaagtct ccctgaagac ccgttttctt
   123961 tttgtaattt tctccaaccc tgtttataaa ttcttctatg gcttcctctt taacaagaga
   124021 aatcgtacag cctccaaaac cagctcctgt catcctggaa cctaaaacgc cttcttgttt
   124081 ccaagcttct tctaccatac tgtccaattc aaatcctgtt acttcatata aatcccttaa
   124141 agattcatga gattggttta aaagcttacc aaaggtcatg atatcccctt gactcagttt
   124201 ttccacagct tccttcaccc tggcattttc ataaatcaca tgttctgctc tttttcttat
   124261 gactggatcc aaaattatat ccttattttc ttcccactga ccaggcgtca gattgcataa
   124321 agattttata tccagcctgt tttgtaaagc tgcaagggcc ctttcacatt cccttcttct
   124381 ttcattatat ttagaatcag ctaaagtcct ttttttattg gtattaccaa taaccagttt
   124441 tattcctttt aaaggaattg ggacatattc gtaatttaaa ctgtcacaat ccagatatat
   124501 ggccatattc ttttttccca tgcctatggc aaattgatcc atgataccac aattgactcc
   124561 tacaaattga ttttctgcgt cctggcaaag ttttatcata tctattatgg aaatatcaca
   124621 atcaaagaaa ctctttaata tcattgctgt caccaattct atagatgcag aagatgaaag
   124681 gcctgcacct gttggaatag ttccataata taaggcttca aaacctccca gagtaaaacc
   124741 tcttcttaaa aattggtcaa ttactccctt gggataattc ccccaggcat ctttattttc
   124801 aaatgtaata cggtttaaat ccagtgtgac ttgcaatgga taattcatag atgccagttt
   124861 aatgatagaa tcctttcttt ttcttgccag ggcagttgtc ccaaaatcca gggcacaggg
   124921 cagtaccctg ccgccgttat aatcaatatg ttctccaatt aaattaatcc ggccaggggc
   124981 aaaaaataca tctggttttc ctatttctcc aaaaatctct gtgaatttac tcataagctg
   125041 ttttgtattc atcgtctgtc ctccttaaat ggtctgactt tgattattca aataactaaa
   125101 tttttgccgg agcggcacag gaatccctga taatcaaatg ggtatccatt atgatttttt
   125161 taaaatttgt agctgtatct tctatgattt caatcagaag ctttgctgcc gtttccccca
   125221 gggcgtacat attttgatct acggttgtaa gcctgggttc acttaattca gaaattaaga
   125281 tattatcata gccaataacc gataagtcct tgggcacgga ttttcccagt ttttttgctg
   125341 cctgtacagc acctatcccc atccagtcat tgcaagcaat aattgctgta ggtccatttg
   125401 ccttttcaag cctttcttct acaatttcta tagttaaggc aaccgtctcc aggccatttc
   125461 catctttaat ttttagtata ttttcttctt taacagcaat tttatgcttt tttagcagct
   125521 cgtaatataa agcttctttc aaatcataag aataactgtc ttttcccctt aaaaaggcaa
   125581 tgtctttatg acccagacca atcagatatt ccatagcctg aatcatgccc atttcctggt
   125641 cgcttaaaac aaaatttgcc cgaattccct tatgataacc attcacaatc actaagggaa
   125701 tgttcaaggc tattttttca tagaaatcgt tttctatatt atccatcaaa ggatctatga
   125761 ctaaaatacc atccacctgt ttttctatga gattttgaat ttgttttttt tccttttctc
   125821 ccctgctgtc agtatcgcat aaaaacaagc taaaaccttc ccgatcagat atattttgta
   125881 tgccttttac aaccacagga aaaaataaat tcgtaatact gggtacaaca actccaagac
   125941 ttttcgtctt tttattaata agacttcttg ctaaaagatt gggcttataa tccaattctt
   126001 taatggccct ttctactttt ctcctggttt cttccttaac aggataatta ttattgataa
   126061 cccttgatac cgttgtaata gaaaccccgg caagtcttgc cacatcactt atggttgctg
   126121 ccattatttc acctccagga tagaaaacct tttctatatt gagaatatca tataaacttc
   126181 tttttttcaa gtaatctttt tcatagaatt caaatttggc tccaaaaaaa taataaaaaa
   126241 ggatagacaa atgctatcct tcctttgcac aaacattgat tatttccatg attttttcaa
   126301 ttccattgga tataccgcta tttttcatgc tttcaatata tttttccctg ttcttataaa
   126361 cttccataat ggaattaaat aagctttctt ctgtcaggct ttcctcttct aatacctgac
   126421 tgaaaccttg tttataaaat gaacgggcat ttaaaatctg atctcccctg cttgctcttg
   126481 ctgataaggg aatcaggata ttaggctttt ttaaactgac tatttcagca atggaattgg
   126541 cccctgccct tgaaattaaa agatcagcag ctgcaaataa atgcggaagt tctttatcaa
   126601 catattcaaa ttgcttatag ccagctatgt catttaatga gtcctttata ttatttttcc
   126661 cgcaaatatg tataacatta aaaggctcta ataaaacagg taatatattc ctaagaaccg
   126721 tatttaaaat aatcgatccc tgactgcctc ccatcatcat aatgacaggc ttatcgtttt
   126781 taaaattaca aatcttccgg cctgtttccc tgtctcctct atataattct tctcttaaag
   126841 gtgttcctgt aagaattccc tttgattttg gaatatagga taaagtttca gggaagttcg
   126901 cgcaaattac ttttgcaaat ggaaaagcca gcttattagc aagacctggc gtaatgtcag
   126961 attcatgaat aataaccggt acccttttaa gccagcctgc cataacaaca ggaaccgtca
   127021 cataacctcc ctttgaaaaa ataactgagg gctttaattt gttaattaat tgaatagcct
   127081 caaataaacc agcaataact ctaaaaggat ctgttaaatt ttttatatcc ttataccttc
   127141 tgagttttcc tgatgaaata ccataataag gaatcatctt ttcttctatt agttcttttt
   127201 ctattccagt tttactgcca atgtagcgga tatcccagcc ttctttttga agttttggaa
   127261 tcagagccag attgggcgtc acatgaccag ccgtaccgcc accagttaat ataatccttt
   127321 tcaaatggat cacctcttca ttctttatta tttatcccgg aacctttgcg atcgttttcc
   127381 ttctaaaata tataaatgct ttattattaa cagaaagaat taaccatctg ctgtccccct
   127441 gctaaaaaat ttataaaaat ccattattat gcatttttgt atcatataag actattttaa
   127501 tataattata tcatagtgaa aaaatttagt ctatttattc acttttcact aatgattttt
   127561 aactcatttt tagtcctcca aaagtttatt atatgttatt ttttgagcat aataaataat
   127621 gaacttttat tgatttataa aaaaaagtat gttaaagttt acatatgttt tttgagtttt
   127681 acattttcta tttctgaaag gggaaaacac atgtccggtt tttgtggatt tattggcaat
   127741 actgtggata aagaacaaat attaaaagat atgaccaatg ctattattca tagagggcct
   127801 gatagtgacg gcgaatacat agatgatacg atagccattg cttttagatg tcttagcatc
   127861 tcaaatcctc aggatgatgc acaaccgctt tttaacgaag atgaacagtt ggtcttagtt
   127921 tccaacagca gaatatataa ttatgagtcc cttaaagaat tattggaaga aaaagggcat
   127981 ctttttaaaa gccagtccga tgcagaagtc cttattcatg cttatgaaga atttggtaca
   128041 aaaatgctgg accatctgag aggaatgttt tcttttgtaa tatgggacaa aaaagaaaaa
   128101 cgcttatttg gagcaagaga tatctttggc attaaacctt tttactatta taaagataat
   128161 gattacttta tttttgcttc agaaatcaaa agtattctaa aacatcgttc agtgcacaaa
   128221 gaattaaatc ctgatgcctt agaaaactat ctgtctttcc aatattctgc tttagaagaa
   128281 actttcttta aaaatattta tcgattacct ccagcgcatt tctttattta taataataat
   128341 gaaatgcatc tacaaagata ttgggaaccg gtatttgaaa cggaaaataa atctttggaa
   128401 gaatatgttg acgaaattga taagaccata caagaatcta tagaacttca taaaatgggc
   128461 agcgcagagg taggttctct attatccagt ggggtcgatt ccagctattt agcctcatgt
   128521 tttggaggtg ataaaacctt caccgtaggt tttgattatg accaatacaa cgaaattgat
   128581 tatgcaaaaa atctgacaaa agaaatcggt attaataact atcacaggct tatcaccact
   128641 aaagagtatt gggacatcct tcctaaagtt caatattaca tggacgagcc tttagcagac
   128701 cctgctgcta ttgctcttta ttttgccagt gaactggcaa gggaacatgt aaaagttgtt
   128761 ttttccggtg aaggtgctga cgaatttttt ggcggctata atatttataa agaacctctg
   128821 gatatcaaag tccttaccag tcttcccatg cccataagaa gatttcttgg aaaactggcc
   128881 tctatacttc cttttgatat taaaggaaag aacttcttta taaggggcag taaaagagtt
   128941 gaagaaagat ttattggaaa tgcttttata ttcacagaaa aagaaagaaa agaattgctt
   129001 aaacataaaa gtaatgctgc ttctcctttt agtctgacta aggctttcta taataaagta
   129061 aaagataaag atgatattac caaaatgcaa tatatcgaca ttcatatgtg gcttccggga
   129121 gatatcctgc ttaaagcaga cagaatgacg atggctcatt cccttgaagt aagggcacct
   129181 ttattagata aagaagtatt taaccttgca gcgaaacttc ctacaaagta taaagtcaat
   129241 aaaaaaagta caaaatatgc cttcaggctt gcagcaaaaa ggcatatacc tgaaaacgta
   129301 gccaataaaa agaaattagg ctttcctgtg cctacccgtg tatggctcag agaagaaaaa
   129361 tattattcca ttgtaaaaga agcctttagt tctcctatag ctgaaaaatt ctttaataca
   129421 gacaaattac tcttacttct gaatgagcat tattcaggca aaaaggacaa cagcagaaaa
   129481 atttggaccg tctatatttt cttaatttgg tatcagcaat attttgaagc ataaaaaacc
   129541 taggttataa cctaggtttt tctttttaca agcattaagc ttgtaagctt aatttacgta
   129601 tacatatctc ttttattgta aagacttata caatttattc gctaaatcag tcaattcctc
   129661 attcgtcggt tttaatggtt tccatgtaat atttacttca tcatcctggt atctgggaat
   129721 taaatgaaga tgaaaatgga atacactttg tcctgcaatt ttaccattgt tctgtaaaat
   129781 gtttaagcct tcaatattga gctccttttt aaggatcttt gcaatattaa ttgccaaaga
   129841 gaataacttc ccgcctaatt ctgaatccat ttcatagata ttttccatat gttctttcgg
   129901 tataatcagt atatgcccct tattagcagg gaatttgtct aaaatcactt taaattcgct
   129961 attttcaaat atcgtcgctg aaggtatttc tccttttgca attttacaga ataagcattc
   130021 ttgtgaattc aaagtatttc ctcccttatt taagtccgta agcctatatt atagcaatat
   130081 tatagcatat tttttgaaaa gtaaaaataa aaaaattggc taag
//
LOCUS       DAOSUD010000002        60992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_334_length_60992_cov_27.591677, whole genome shotgun sequence.
ACCESSION   DAOSUD010000002 DAOSUD010000000
VERSION     DAOSUD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 60992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60992
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_60992_cov_27.591677"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..156)
                     /locus_tag="PLG49_00545"
     tRNA            complement(65..156)
                     /locus_tag="PLG49_00545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Ser,seq:gct)
     gene            complement(257..1300)
                     /locus_tag="PLG49_00550"
     CDS             complement(257..1300)
                     /locus_tag="PLG49_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOQ15832.1"
                     /translation="MKKKAIVFWIFVLGLVTAYLIVYSKQLKEISQASKMIQNDRSAN
                     IQEPTVFEVYYKNKNFKSFKTKEEAIEYSKKFKYTYVKASNSDKWIYNNFKPFILFDG
                     DLFINDYDSFADAVFVAREKENSVIYFKNNGNIIWSKTEKLQNNVLLDVPVILQYPEL
                     PRGCEVTSLAMLLNYAGIEVDKIELAKKIKKDETPFSIKGNRVYFGNPNDGFVGDMYS
                     LKAPGLGVFNGPVEELMKEYLPDETINLTGCEFEDLFHFISRGYPVWAITNTMYVKLD
                     NSEFEIWMTPNGPVEITYKLHAVVITGYDENNIYLNDPFYNQKNIKVNKERFKESWNQ
                     LGRQAISYIGLRK"
     gene            complement(1330..3774)
                     /gene="gyrA"
                     /locus_tag="PLG49_00555"
     CDS             complement(1330..3774)
                     /gene="gyrA"
                     /locus_tag="PLG49_00555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOQ15833.1"
                     /translation="MEEKELKKRIIPVEIEEEMRKSYIDYAMSVIVSRALPDVRDGLK
                     PVHRRILYAMNELGLTPDKPHKKSARIVGETMGKYHPHGDSSIYDAMVRLAQDFSIRY
                     PLVDGHGNFGSVDGDSPAAMRYTEARLSKIALELLADIEKDTIDYNPNFDESLKEPAV
                     LPSRFPNLLVNGTSGIAVGMATNIPPHNLTEVINGVVKIIDNYVEENRETDIEELLPI
                     IKGPDFPTGAVILGRRGIEEAYRTGKGKIKVRAVSEIESMSNNKQRIIVTEIPYMVNK
                     AKLIEKIAELVKEKRIDGISDIRDESNREGMRIVIEIKKDYNANVVLNQLYKYTQLQD
                     IFSVNILALVNNEPKVLNLKDMLIHYLEHQKDVVTRRTQFDLRKAEERSHILEGLKIA
                     LDHIDEVISIIRSSQNTPEAKQRLIESFALSEQQAQAIVDMRLRTLTGLEREKIDEEY
                     RELQETIKELKAILASEKRLYGVIKEELLIILQKYGDDRKTKFAHDDGEIDIEDLISD
                     EQNVVTLTRLGYIKRLPLNTYRSQNRGGRGIIGMQTREEDILEELFVTSTHSYILFFT
                     NKGKVYRLKTYEIPEASRTARGVAIVNLLQIDGDKKITAIIPVKEYKEGSYLLMLTKN
                     GIIKKTSINEYENIRKSGLNAINLRENDELIQVINIADEDEIFIGTKFGQGILISGKE
                     IRAIGRNATGVRAMNLNEGDEIVGAGLISEGKEILFVTEKGLGKRTDINEFKLQRRGG
                     KGVKCYRLTEKTGKIIGIKMVSEDEEIMMITSEGIIIRMHVSQISKTGRVTQGVKLMN
                     LSEDEKVVAVAKVVEE"
     gene            complement(3798..5699)
                     /gene="gyrB"
                     /locus_tag="PLG49_00560"
     CDS             complement(3798..5699)
                     /gene="gyrB"
                     /locus_tag="PLG49_00560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOQ15834.1"
                     /translation="MVPGYDENQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVD
                     NAIDEALAGYCDEIKVTINKDNSITVIDNGRGIPVGIHPQKKIPAVEVVFSTLHAGGK
                     FGGGGYKVSGGLHGVGAAVVNALSKYLKVQVFTDGKIYEQVFERGKLVQPLKVVGETD
                     KRGTLVHFLPDDEIFEEVEFQFDILRQRLRETAFLTKGINITLTDLREENKKEKVFHY
                     EGGIKEFVAHLNKHKDVLYDEIFYCEGRKDNVEVEIAFQHNDTYVENIFSFVNNINTT
                     EGGTHLIGFKSAITKTINDYGRKANLLKETDKNLSGDDVREGLTAIISVKVPEPQFEG
                     QTKTKLGNSEVRGIVEAVLSEELMYFLEQNPTIAKTILQKGIMAARAREAARKARDLT
                     RRKSALESNSLPGKLADCSERDPKLCEIFIVEGDSAGGSAKSARSRQTQAILPLRGKI
                     LNVEKARLDKILGNEEIRAMITAFGTGIGEEFDIEKLRYDKIIIMTDADVDGAHIRTL
                     LLTFIYRYLRELIEKGHVYIAQPPLYKVEKNKKVYYVYNDKELEKLFSEIGRDNIKMQ
                     RYKGLGEMNPEQLWETTMDPESRILLKIDLNDAAAADEVFTALMGDKVEPRREFIREY
                     AKTVKNLDI"
     gene            complement(5730..5990)
                     /locus_tag="PLG49_00565"
     CDS             complement(5730..5990)
                     /locus_tag="PLG49_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOQ15835.1"
                     /translation="MFLHIGADTEIRVKDIVGIFDIKSLMTSKTAQSFLNIVQNEDLH
                     KLISKEIKSVIITEENGKSLFYFSPISSVALQKRISHYQTGF"
     gene            complement(6021..7142)
                     /gene="recF"
                     /locus_tag="PLG49_00570"
     CDS             complement(6021..7142)
                     /gene="recF"
                     /locus_tag="PLG49_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOQ15836.1"
                     /translation="MYISEIDLYQFRNYDNTNIKLHPKLNILYGNNAQGKTNILEAIY
                     MCATARSHRTSREKETIKWDKEEAHIRLFLNKGDRLDKIDLHLNKNGKKYVFINEVPV
                     QKLSNLLGIMHIIMFSPEDLQLIKSGPKERRRFIDIELCQIDKIYLYNLQQYYKVLKQ
                     RNHLLKNINYDKGQEAMLEVWDEQLVFYGEKIIETRKHFINKISHIASVIHGNITSNK
                     EYLEIEYFPNVEKENFKDKLKSFLEKDIKTGITSIGPHRDDVLFKINHNDVRTYGSQG
                     QQRSVILSLKLAEIDLIKNEVKDNPILLLDDVLSELDENRQADLLSNISDIQTILTCT
                     GIEDIILKKLHKGFLFYIENGFVEPRDSNSCNISINKIK"
     gene            complement(7156..7365)
                     /gene="yaaA"
                     /locus_tag="PLG49_00575"
     CDS             complement(7156..7365)
                     /gene="yaaA"
                     /locus_tag="PLG49_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HOQ15837.1"
                     /translation="MEKVKISTEYIKLDQFLKYVNIVASGAEAKYFILEKDIKVNGEI
                     VKQRGKKLKNGDKVEIDGNEYIIIN"
     gene            complement(7472..8575)
                     /gene="dnaN"
                     /locus_tag="PLG49_00580"
     CDS             complement(7472..8575)
                     /gene="dnaN"
                     /locus_tag="PLG49_00580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ15838.1"
                     /translation="MRLTCSNVDLLNGVNIVSKAVSNRTTLPILECILLEAEGNYLKL
                     TGNDLEIGIESKVIANVEKTGSIALEAKIFSEIVRKLPDGEVLISVDSNNMTTIKCEN
                     SEFKISGQNGDNFPELPKVEKDKHFVLKQAVLKDMIRQTIFSIATEETRPVLTGELFQ
                     IRDNHLRLVSVDGYRVSYRQAPLSVEREEQEVIVPGKTLGEINKILSSDEEDVYIYFT
                     NKHILFDLGDSMVVSRLLEGEFLKYENSFFEDCETMIQVNRRELLMSIERAALITREG
                     RKNPIKIDITGDKMIITSNTELGTAKEELPVDTEGKDLTIAFNPKYLIDALRVIDDEE
                     IKIQFISSLNPCIIKPVDNEDYKYLILPIRLNG"
     gene            complement(8821..10158)
                     /gene="dnaA"
                     /locus_tag="PLG49_00585"
     CDS             complement(8821..10158)
                     /gene="dnaA"
                     /locus_tag="PLG49_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ15839.1"
                     /translation="MLNQITKQWPSILEILESETSTVSFQTWFKNLKPVDVRDNTLFI
                     ETIDDFSKDILKNRYIRLIQNAIIQVTHHEFEIQIVTSNEFNQSQKYNSIKDNDTDPL
                     DNQSNLNPKYVFDTFVIGNSNRMAHAAALAVAEAPAKAYNPLFLYGGVGLGKTHLMHS
                     IGHYILSQNPNVKVLYASSEKFTNELINSIKDDKNQAFRNKYRNIDVLLIDDIQFIAG
                     KERTEEEFFHTFNTLYEANKQIIISSDRPPKEIKTLEDRLRSRFEWGLIADIQPPDLE
                     TRIAILRKKAEIEKLIVPNDVILFIAKSIVSNIRELEGALNRIIAYSTLANKEITVDL
                     ASEALKDLMSADKPKTITTELIQEVVASYYNIKPEDLRSPKRTRNIAYPRQIAMYLCR
                     KLTDLSLPKIGEKFGGRDHTTVIHGYEKIEKEILQDFDLKQTIEDLEKRITGK"
     gene            10656..10790
                     /gene="rpmH"
                     /locus_tag="PLG49_00590"
     CDS             10656..10790
                     /gene="rpmH"
                     /locus_tag="PLG49_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOQ15840.1"
                     /translation="MKRTFQPKKRQRQKEHGFRKRMKTASGRAILARRRARGRKRLSA
                     "
     gene            10868..11206
                     /gene="rnpA"
                     /locus_tag="PLG49_00595"
     CDS             10868..11206
                     /gene="rnpA"
                     /locus_tag="PLG49_00595"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ15841.1"
                     /translation="MKFTDSLKKNYQFRIVYNKGISSANRLLVMYLLKNGKDINRLGI
                     SVSKKVGNSVKRNRITRLIRESYRLIEDNIDRGWDIVIIARLPCNGSNFKEISDALIH
                     LLKKNNLYIA"
     gene            11222..11443
                     /gene="yidD"
                     /locus_tag="PLG49_00600"
     CDS             11222..11443
                     /gene="yidD"
                     /locus_tag="PLG49_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOQ15842.1"
                     /translation="MNIFLKKILLFMISFYRKFISPLKPAVCRFYPTCSAYSYQAIEK
                     YGVGKGLYLSVRRILKCHPFHPGGYDPVP"
     gene            11466..12437
                     /locus_tag="PLG49_00605"
     CDS             11466..12437
                     /locus_tag="PLG49_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOQ15843.1"
                     /translation="MIDALAKILGVVFNFIFDLVYKVTPVGSLGITIILFTILVRLLM
                     IPLVAKQQSSLKRMQEIQPELQKIQDKYKNKQDPESQRQMSIELNEFYRKHNVSPFSG
                     CLLLFIQMPIFIALWRVLQKAGTYISKLHDLYYNLASQIVSADGYQEIVKNILSGNKR
                     IKLNDEILASAEGLQSILSQVSTSEWNKLSETLGTISNTLIEQKNSIEYFFGISLVDS
                     PADLIKQGLNIPALIVVILFPIMAALTTFLSSKLLSMNNKNNKQKQNDQTEQIQKSMN
                     LVFPVITGMMTYSLPAGLGIYWITSNIIQIIQQYVINKSMDKSGEGE"
     gene            12441..13070
                     /gene="jag"
                     /locus_tag="PLG49_00610"
     CDS             12441..13070
                     /gene="jag"
                     /locus_tag="PLG49_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOQ15844.1"
                     /translation="MDFVEKESRTVEDAITEGLIELGTTRKNVEIEILDKGSRGLFGL
                     IGAKMAKVRLTKKVNPVDLAVNFLKDVFDKMNLDAEIIVEEKEEGNYVFNIQGDDMGI
                     LIGKRGQTLDSFQYLANIVANKNKNNHIHITLDTENYRERRKETLETLAKNLAKKVKM
                     TKKSIVLEPMTPYERRIIHFALQNDKHIKTHSEGEEPYRKVIISPVIDK"
     gene            13125..14501
                     /gene="mnmE"
                     /locus_tag="PLG49_00615"
     CDS             13125..14501
                     /gene="mnmE"
                     /locus_tag="PLG49_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOQ15845.1"
                     /translation="MIDDTIAAISTPPGVGGIGIIRVSGRDAFSIVNSIFRSKKVKDL
                     SSVKSHTIHYGHIVDGDSLIDEVLVSVMKAPNTYTKEDVVEINCHGGMVSVKKVLEQV
                     LKNGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIINSKTENSLKLAVNQLEGRLSKEI
                     KKIMDKIIEIMAHIETSIDYPEYDLEEINYDNLENNIKEIRKEIRHLLETADSGKIMR
                     EGVKTVIVGKPNVGKSSLLNALLREQRAIVTSIPGTTRDVLEEYINISGIPFKIIDTA
                     GIRETNDIVEKIGVDRSKELLELADLIIFIIDLSVPLTEEDRYIMDLVKERKTLVILN
                     KTDKETVINKKEIYDKFINNKVLEMSTKNPEDIERLEETLKEMFDEGHFNIDDSAVIS
                     NMRHKNALIKANNSLEDVLKSISMNMPVDFLSIDLSDAYNFLGEITGETIEEDLINKI
                     FSEFCLGK"
     gene            14528..16423
                     /gene="mnmG"
                     /locus_tag="PLG49_00620"
     CDS             14528..16423
                     /gene="mnmG"
                     /locus_tag="PLG49_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOQ15846.1"
                     /translation="MGYVVSDIDVAVIGGGHAGCEAALATARMGLNTVLFAISLDSIA
                     MLPCNPSIGGTSKGHLVREIDALGGEMGKNIDKTFIQSRMLNTGKGPAVHSLRAQADK
                     HNYSKQMKMTLERTENLRIKQAEIVKIHVENNKVKAVETATGAIYNCKAAIIATGTYL
                     KARCLVGDVIEYTGPNGLKAANNLSENLKELGIELFRFKTGTPARVDRRSIDFSKLEE
                     QPGDEKIVPFSFSTNAKDIQREQISCWLTYTSEKTHQIIKENLHRSPLYAGEIKGVGA
                     RYCPSIEDKVVRFADKERHQVFLEPEGENTTEMYVQGMSSSLPEDVQIKMLRTLPGFE
                     KCEVMRVGYAIEYDCINPIQLRHTLEFKKIDGLFSAGQFNGTSGYEEAAAQGIVAGIN
                     AALKILGKEPLYIDRSEGYIGVLIDDLVTKGTNEPYRMMTSRAEYRLLLRQDNADLRL
                     MEKGYRVGLVSKERYEKLLEKKRLIDEEINRLKKTVIGVNQKVQDFLKAHNSTPIKSG
                     ITLSELIKRPELDYYKIREIDKDYVDLPEDVQEQVNIQIKYEGYIKRQMQQVEQFKKL
                     EKKYLREDIDYTKIKGLRIEAAQKLQQIKPRSIGQASRISGVSPSDISVLLVYLEQVS
                     RNKNTNQ"
     gene            16434..17153
                     /gene="rsmG"
                     /locus_tag="PLG49_00625"
     CDS             16434..17153
                     /gene="rsmG"
                     /locus_tag="PLG49_00625"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOQ15847.1"
                     /translation="MELKDYLIEGSKQLGVHLEDYQTEQFFQFKDLLLEWNNKINLTA
                     IVDDKEIIIKHFLDCLSIGQFFDCQTACNVIDVGTGAGFPGIPLKIAYPNLKTTLLDS
                     LNKRISFLKEVVLNLQLKDVTCIHGRAEELGQNKEYRELYDLCVSRAVAHLSVLSEYT
                     LPFIKTGGTLIAMKGLNVDEELNESKKAIEVLGGEIIDIKDVVIPFSDIKHRLIMIKK
                     VRLTPTKYPRKAGKPGRNPIK"
     gene            17277..18083
                     /gene="noc"
                     /locus_tag="PLG49_00630"
     CDS             17277..18083
                     /gene="noc"
                     /locus_tag="PLG49_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOQ15848.1"
                     /translation="MQAEKSKELKIQWIPIDKIRPNPYQPRKNFDRAALDELTNSIKE
                     YGVMQPISVRLIGKSNYELVAGERRLRACKNAGLSEIPAVLVEVNDKESALLSLIENL
                     QREDLNFIEESEAYFNLIQDYGFTQEELARSLGKSQSTIANKLRLLKLSPTVKKIILE
                     NDLSERHARVLLKLPDEDMQLEVLEKVIIHGLNVKNTEELVEEMIERIIHESEEKKQQ
                     KMKRVLRDIRLFTNTINQAIDLIKKSGVEAKYTMEEKEDHYEIKIKIPIY"
     gene            complement(18122..19069)
                     /locus_tag="PLG49_00635"
     CDS             complement(18122..19069)
                     /locus_tag="PLG49_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ15849.1"
                     /translation="MKNKKKLLNFAMILLSIQLLNQLIFLTSKLKFNKIKKSYHWYPW
                     KFGKIHYRVQGSGPPLLLIHSTGNGASSCEWRKNIPYLSKNFRVYAIDLLGYGLSAKP
                     KLTYTAFLYVQLINDFIKDIIKEPAYVIANSQAASFAIMSSSYNPSLIKKMILIAPTG
                     INKLASYPTQKSKLMKLFIELPFIGTSIYQMISSKLYTYYFMQKYLYNSKKLISKDLI
                     DTYHGMSHDKGPSSRYTLASFIGGYMNIDVKKAFSKMKTDVLILWGKDNTLVPLSLLE
                     EWKKVKASVSTYIFKNSKAMPHEEEALTFNQICETFLLK"
     gene            complement(19989..20732)
                     /locus_tag="PLG49_00640"
     CDS             complement(19989..20732)
                     /locus_tag="PLG49_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15850.1"
                     /translation="MKKILIYTTFLSILFISLSQLKHKEDSNSISYDKSKLKEKILIV
                     EIEDFNFNQLKNKGVYSLLHVYPRKNFKDLYFDLKRKQDVTLMNFNEYIKREYSSISP
                     IIILEKVKINQFEQITNFKKDDTSLIIVNLEKQMNPLFIESDLNGILQSNFCKREGII
                     KYTELINIINNHLESGSLSVQNTKKANEKIQKIYYQYTSLYYGRYILITFILIQFTLF
                     VFSKQNKILFQYFIFIPFFFVFCIRFKYI"
     gene            21114..22268
                     /locus_tag="PLG49_00645"
     CDS             21114..22268
                     /locus_tag="PLG49_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOQ15851.1"
                     /translation="MAICMKYNEMDKLDQILNRTIESIEKSKTEMYEIYEKSYREYLS
                     LKNELSELKKKIRLSTRLIEGYEKQLARSKVNLMEVSKNFDQSTEEKIKEVYDETDQL
                     RIDLAVEKEREKSLINKRNELEVRIKALKEIVNKANNLINNVSVAMKFLTGDLKNLSS
                     QIGDIKEKRNIGIKILIAQEEERRRIAREIHDGPAQYMSNLVLKAEYCIKLLDNNLDE
                     AKMELGKLKELLRSGIQELRGIIYDLRPMSLDDLGLVPTIERYISKVMEEKDIVIKLS
                     ASGSKQNISNVIKLTIYRLAQEAISNIIKHSNATEAKIKLTFLQNYIELIVSDNGEGF
                     DISELKKQRIDGESGFGLCSMKERTELLDGKFVIQSKPKKGTRYYFRIPC"
     gene            22325..22987
                     /locus_tag="PLG49_00650"
     CDS             22325..22987
                     /locus_tag="PLG49_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ15852.1"
                     /translation="MKDIVSVIIADDHSMVREGLKQLIELEDWIRVVDQAANGLEVIE
                     KAYNKNPDIILMDINMPVLNGIEAAKRLKRNACHSKIIMLTIHNEVEYLFETVEIGVD
                     GYILKDSESEVLINAIKSVYNGKSYIQPNMAAQLVKRVNSIHGDFNGEDHNKKHLTKR
                     EIEVLKLITEGMLNKEIAQKLCISEKTVKNHVSNIFKKINVCDRTQAAVFAIKENIVN
                     IN"
     gene            23127..24830
                     /gene="argS"
                     /locus_tag="PLG49_00655"
     CDS             23127..24830
                     /gene="argS"
                     /locus_tag="PLG49_00655"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ15853.1"
                     /translation="MMDFKLEIAKLLSGQFTDLSLEEILSMIEIPPNPDMGDYAFPCF
                     RLAKILRKAPAQIAEDAVKNIEKADFIDEIKNVTGYINFYINKSVFAAAVLSELLKAK
                     ESYGQSDIGKGKNIVIDYSSPNIAKPFHVGHLRSTVIGGSIYRIFKALGYNTIGINHL
                     GDWGTQFGKLIVAYKKWGSKEAIEKGGIKELMDIYVRFHSEAKEDPSLEDEAREWFAK
                     MEAGDEEALSLWNWFKDISLKEFNRVYKLLDISFDSYAGESFYNDKMDAVIKELEEKN
                     LLKESEGAKIVDLEDANMPPCLITKKDGSSLYATRDITAAIYRKKTYDFEKCIYVTAL
                     AQNLHFAQWFEVIKRMGYDWHDKLVHVSFGMVSMETGKLSTRAGNVVLLEDLLNEAIS
                     KTLDIINEKNPDLEDKEQVAEKVGIGAVIFNDLINNRIKDVVFSWDKVLNFDGETGPY
                     VQYTHARASSVLRKANVDIDSLDIDYSLLTDDSSIEVIKLIGQFPEKVKDAADKLEPS
                     FIARHIVDLAQAFNKFYHDNPVLVEDEKIRNARLALVYSVKTVIKSGLYLLGIAAPEK
                     M"
     gene            24842..25738
                     /locus_tag="PLG49_00660"
     CDS             24842..25738
                     /locus_tag="PLG49_00660"
                     /inference="COORDINATES: protein motif:HMM:NF009605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOQ15854.1"
                     /translation="MKKIKLFYNPVSGDKKFPQQLDTFIYKFQNAGYEVAIYRSEFNG
                     DITKAVKKIVPDEYSILAVAGGDGTINEMVNGMVEAGIDIPLAIFPVGTANDFANHLN
                     LPKSVADCCDVVLRNNIKEIDIGRVNNKYFINVCSGGLFTSVSQSVDLNVKNTLGKLA
                     YYIKGIEQLPNFRPFKLRIETSGDTIEDFFYLFLVLNGNGAGGFERLSRDASVQDGLF
                     DFVGVKAKPFHELAILFLKILKGEHLDDPNILFIRDKKFKITCLEEDRRFYESDVDGE
                     KGPDLPLNIVVVEKKLKVFTNL"
     gene            26037..26804
                     /locus_tag="PLG49_00665"
     CDS             26037..26804
                     /locus_tag="PLG49_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ15855.1"
                     /translation="MGTTIAVFNQKGGVGKTTTTVNVSAGLAEKGAKVLAVDIDPQGN
                     TTSGLGVDKNKLKKTIYDVLLGDLSMKEVKKPTPMKNLDLIPSNMDLAGAEIELLDIN
                     KREYILRKNLEDIKNDYDYILVDCPPAVNILTLNALAAANSVLVPIQCEYYALEGLTH
                     LISTIKLVQQRINPELYIEGIIFTMYDARTNLSLQVVEEVKRYFPKEIYTTLIPRNVR
                     LGEAPSHGLPIMLYDEKSKGAESYRLLVEEILERKEA"
     gene            26801..27655
                     /locus_tag="PLG49_00670"
     CDS             26801..27655
                     /locus_tag="PLG49_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ15856.1"
                     /translation="MVAKRGLGKGLSALISSEMSEENQIKMISINSIEPNKEQPRKYF
                     DEDKLEELASSIKEHGIIQPLIVKQEEDYYVIVAGERRWRAARLAGLKEIPVIIQDYT
                     TKELMEISLIENIQREDLNAIEEAKAYETLMNQFSLTQEELAGRIGKSRPAIANTLRL
                     LQLDEKIQELIIQGEITEGHGRAILRLPDKEAQLALAKKIIKDHLSVRETEKKVDEML
                     KKPKKKTIKDEKEVIYKEIEDKIKNILGTKVQITRGKKKGKIEIEYYNENDLERILYL
                     LESIREAN"
     gene            27667..28176
                     /locus_tag="PLG49_00675"
     CDS             27667..28176
                     /locus_tag="PLG49_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOQ15857.1"
                     /translation="MNLIKILLDNQVWLLITTSIISLVLLVITILLMIKINCLQKKYD
                     YFMGGEKEKPIEDLLLAYIDRVSQVEKDNKVISRDIKNIRKDLSKCINKVGIIRYNAF
                     ENMGGDLCFAIALLDENDDGIVINGIHSRDGSNTFAKPIKKGTSAYTLSKEEKEAIEI
                     AQSNSKMNI"
     gene            28188..28499
                     /locus_tag="PLG49_00680"
     CDS             28188..28499
                     /locus_tag="PLG49_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HOQ15858.1"
                     /translation="MEIIYIVNPTAGNGKAKMLIPKIHEIMKDKNLSYQVIETTKKGD
                     ATLLAKKAVNEDAKKVIAVGGDGTINEVVNGIEGSKVVLGIVPAGSGNDVARLLKLPK
                     K"
     gene            28589..29059
                     /locus_tag="PLG49_00685"
     CDS             28589..29059
                     /locus_tag="PLG49_00685"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15859.1"
                     /translation="MGLDAEIAAYTQVSKKYFKGKWAYIASLIKNLFCYKPIHAVITI
                     DGKEMERNLLLFAVGNGQYYGGGFKILPQAIMDDAYFDACIVSHIPRWKIPLALPALI
                     KGEHDKYPFIEFIKLKKIKIKTSKEVKLNLDGEISQGSYFEFEIIPNALNIIGH"
     gene            29109..29399
                     /locus_tag="PLG49_00690"
     CDS             29109..29399
                     /locus_tag="PLG49_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HOQ15860.1"
                     /translation="MIIGIQRGLDGLKKKLEEKGYQVYFEDEYPYAIDAFIFNKSLTL
                     PDMDYHDSPVLTASLEKIPCQSNKGVLLICSKNKSLEEIEEILNNRVYSPLF"
     gene            complement(29416..30096)
                     /gene="thiT"
                     /locus_tag="PLG49_00695"
     CDS             complement(29416..30096)
                     /gene="thiT"
                     /locus_tag="PLG49_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOQ15861.1"
                     /translation="MFSEEVLNEFFGSLLGKSITAVVTIILIGLFVINLHKKKNLSVK
                     AIAYSGIAIALATILSYIRLFQLPQGGSITPFSMLFIVLVGYWFGPFEGIIAGITHGT
                     LQLLIDGYVVHPAQLLLDYPLAFGALGLSGFFRKGQKSIIPGYIVGSTGRFICSFLSG
                     WIFFGSYAWEGYSAVAYSFLYNLSYIGIEMALTLVILFIPSFRKALNHIRNTVVINES
                     SLTEKKTA"
     regulatory      complement(30136..30240)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            30467..31273
                     /locus_tag="PLG49_00700"
     CDS             30467..31273
                     /locus_tag="PLG49_00700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOQ15862.1"
                     /translation="MNYKMLVTDMDDTLLMDDLTISKENKEAISKALEAGVRVILCSG
                     RATNSMAKYVKELELNKKEEYAISYNGAVIFDIRTLEHIYEENISHEDAKFLFEIGKK
                     NNLFVQTYQDDYVYVEKKTPYSDRYAKISGLEVKEVGDLSKWLKGDVIKVLFQGEKEK
                     LDQLAKELKPVVEGRFHMFFSKPTYLEFTNIHVNKGLTVKRLREKLGLKKDEVICVGD
                     SFNDLYMIKEGGLGVAVANAHPAIRQEADYITKSSNNEHAIKEVIEKFIL"
     gene            complement(31470..31739)
                     /locus_tag="PLG49_00705"
     CDS             complement(31470..31739)
                     /locus_tag="PLG49_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ15863.1"
                     /translation="MDVKAYEKKAEILKALGHPARLCIVKGLLDNDGCNVSYMQTCLG
                     LPQSTVSQHISKLKSAGIIEGVRSGLEITYKVVSKEAIQIINAIF"
     gene            31872..31959
                     /locus_tag="PLG49_00710"
     tRNA            31872..31959
                     /locus_tag="PLG49_00710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31906..31908,aa:Ser,seq:gct)
     gene            32143..32580
                     /locus_tag="PLG49_00715"
     CDS             32143..32580
                     /locus_tag="PLG49_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ15864.1"
                     /translation="MQDLLMDYLTQIISNISQAQSEVLKEKQLKYKISPNELRIIEIV
                     GTSRKPKMMKDIAELMSMTKGGMTFLIDKLEKKGVVRRRQNDFDRRVLYIELTEEGEK
                     IFEDYNRNKYAILYKWVENMNASTKKIMTEEFHRALQLAEKSV"
     gene            32653..32820
                     /locus_tag="PLG49_00720"
     CDS             32653..32820
                     /locus_tag="PLG49_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15865.1"
                     /translation="MDDKKLRLKKDIDLSRNFKDELSAELTDKTTEDNKKDQSEMAFK
                     IIENCNPDDFE"
     gene            32962..33624
                     /locus_tag="PLG49_00725"
     CDS             32962..33624
                     /locus_tag="PLG49_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ15866.1"
                     /translation="MGSLVDSFSLREINFAKTRLNILKVVKDRITQKDFDDITVDEIC
                     KYAEISRGTFFNYFPSKNHIFNYYIRVWMIQLGLEMEKQKFKSSQEKIKKIYDKVIEE
                     DTNYPGFIENYLKYLLESKEINNEIKLTQAELAYKFSLENIKKENMEMFNSLSIEKIF
                     ENFINEGVSNGEFKKDINKENTLLLLLSLFFSPAVMKFINKSFDLKRYYEEALDNIFH
                     NV"
     gene            33685..33758
                     /locus_tag="PLG49_00730"
     tRNA            33685..33758
                     /locus_tag="PLG49_00730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33719..33721,aa:Arg,seq:tcg)
     gene            33811..34683
                     /locus_tag="PLG49_00735"
     CDS             33811..34683
                     /locus_tag="PLG49_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HOQ15867.1"
                     /translation="METYMSAVGFGSIKKKKQWDKLIEEVLNHADKRYVTNHDEDHVF
                     IEYYKSYGKNIGVVLRGLLDNEKDIDFETCDPYFESKYIMEVSEVEVEEDEEDYFYFA
                     ATEEEESGVEIVFQLQNIIEYLEIKDDKHASIEGIKVVGLASKGTIILPVEKSENDLE
                     YENEQKEWRKELLKRIKAGDEEARKILETEAEEAAEIIEERLRHEDILSVIEGYFIPV
                     DYLEATYSILGEIKEIELIINEETREEIYWMLVESMGMSIEIAINANELVGTPLVGMR
                     FMGICWMQGTIVFS"
     gene            34777..34850
                     /locus_tag="PLG49_00740"
     tRNA            34777..34850
                     /locus_tag="PLG49_00740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34811..34813,aa:Arg,seq:cct)
     gene            34990..36195
                     /locus_tag="PLG49_00745"
     CDS             34990..36195
                     /locus_tag="PLG49_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HOQ15868.1"
                     /translation="MKRFKHFLLGFVAGALVFTSPQVKAFDGLQTIKVLFSNIPIYID
                     GNRVELSHKAILHEGTTYLPMRDIAEALGKEVLYNNNTKEIYIYEKEGKNSDKSNTKT
                     DNNDLSRNQQISGEFSVNGIALGDSAEDVSDVLGEPNRQDVSEYGFTWYIYNKDYSNY
                     MQVGIKDNKVVALYTNSVSNWNFKKGIRFGSTAASVRKAYGQPLSFIQKGQYRFYFNY
                     KKGEAETYSIDDAFVTFFYDIHNNQTVTSILIIDKNTELSHESLYGEASEELRTAYTY
                     QIFDLTNAVRVRNGLKALTWDEQAAKSSYLHSKDMAENNFFDHDNLKGEDPFDRMKKQ
                     GIHFMIAGENIAGGQTNAIYAHEGWMNSKGHRENILFENFERLGVGVYFGGRYKIYYT
                     QNFYTPLKR"
     gene            36220..36702
                     /gene="tadA"
                     /locus_tag="PLG49_00750"
     CDS             36220..36702
                     /gene="tadA"
                     /locus_tag="PLG49_00750"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOQ15869.1"
                     /translation="MEDKAITFMKEALKLAEKAKEADEVPIGAVIVYNDEIIGRGYNQ
                     RNSQKNPLAHAEIQAIEEAAKYIGDWRLEGCSIYVTLEPCPMCAGAIVQARIDKIIFG
                     TRNPKAGCGGSILNILNEPRFNHQVEIIEGICQEECSQILKTFFTQMRNKRLKMEVDI
                     "
     gene            36813..37075
                     /gene="ffs"
                     /locus_tag="PLG49_00755"
     ncRNA           36813..37075
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG49_00755"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            37253..38854
                     /gene="dnaX"
                     /locus_tag="PLG49_00760"
     CDS             37253..38854
                     /gene="dnaX"
                     /locus_tag="PLG49_00760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOQ15870.1"
                     /translation="MSYMALYRKKRPKTFEDVIGQEHIVITLKNQIKANRIAHAYLFC
                     GTRGTGKTSTAKIFAKAVNCENSQDGEPCGHCSLCQSIDEGRTMNIIEIDAASNNGVD
                     NVREIREEVKYTPTEGKYKVYIIDEVHMLSTGAFNALLKTLEEPPAHVIFILATTDPQ
                     KIPATILSRCQRFDFRRISTDSITKRLKAYMKEENIEVEDKALSYIARLAEGSMRDAL
                     SILDQCISFNYGERISFEKVLDVMGAVDYKVFFEFASILNKKESLETMELIDKIVMQG
                     RDLSQFVMDFIAHLRNLLIVSSSQEAEKILDLSQEQIELLKDQSKEIASSTILRWIRI
                     FSELSNELKYTDQKRVMLEVAVIKICQPQMDDSKDALLERIKVLEEKLAEGIVVQATE
                     KPKTQENKKSSPKAKPKKPKAVPEDIKKAVSLWNDIKTSFSDSLNGLLMDTKAAYLED
                     DVYYIVFKDAFIIEAIKKYKDEIIARIEELLKKEVNVHFISAEAYQEKYQEIYGDSPE
                     KSEERDQIEEVIEYFEKLGVSYEIK"
     gene            38920..39261
                     /locus_tag="PLG49_00765"
     CDS             38920..39261
                     /locus_tag="PLG49_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOQ15871.1"
                     /translation="MAKRNFGGIPGNMNSLLKQAQKMQKEMEKMQQELEEKDVTATAG
                     GGAVEVVFTGKKELKSITIKPEVVDPDDVEMLQDLILAAVNEGLRQVDELANQQMSKL
                     TGGLNLPGGLF"
     gene            39262..39861
                     /gene="recR"
                     /locus_tag="PLG49_00770"
     CDS             39262..39861
                     /gene="recR"
                     /locus_tag="PLG49_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ15872.1"
                     /translation="MNHYGDTMSRLIAALSDLPGVGVKTAQRLAFHIINMPEENVENL
                     SKAIIEAKRNVKYCSVCCTLTDKEKCDICSDVRRDSKTIMVVEDPRDLAAYEKTKEYK
                     GLYHVLHGAISPMLGVGPKDIKIKELLERLKSDDVEEVILATNPNIEGEATAIYISKL
                     IKPLNIKVTRIANGVPVGGDLEYIDEVTLSRALEGRREM"
     gene            40097..40558
                     /locus_tag="PLG49_00775"
     CDS             40097..40558
                     /locus_tag="PLG49_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15873.1"
                     /translation="MDYHHLMNLNCYIEFCGLTEPETNCLRLYFARCKTEETAEDLII
                     GDTVIKNTYPIIIDKDLPVLQLDFESYIAYSVTNESFTSWDEYEQFEGKAFRIYTKSR
                     YLDFIKEHTFAAQVCVDVYPPFRHYGIVCLNHIINIVSTDTPKITEIYKQQ"
     gene            40650..41153
                     /locus_tag="PLG49_00780"
     CDS             40650..41153
                     /locus_tag="PLG49_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOQ15874.1"
                     /translation="MNDIRKKVIDFLEKMNIPYDMIEHPAVYTIDEMDQLNINISNKE
                     NNEIVKNLFVRDDKKKNYYLIVLQKDKQANFKEIRKALDSRPLTFASEEDLQKYMGLT
                     KGSVSPFGILNDADCKVEIVFDKDILSFERIGVHPNDNTATVWLSLKDLENIIKSHGN
                     SISYINI"
     gene            41724..41927
                     /locus_tag="PLG49_00785"
     CDS             41724..41927
                     /locus_tag="PLG49_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOQ15875.1"
                     /translation="MLIGENGIKVIGENFTEMIPKEIISDIGVYDDMILIFLIGYKSY
                     FIPTRYLTRENKEQFINKLMALK"
     gene            42001..42129
                     /locus_tag="PLG49_00790"
     CDS             42001..42129
                     /locus_tag="PLG49_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15876.1"
                     /translation="MVWRNQSGSTYQEQSCYSFGSVVIINIPDNVLAAGDLCRLSV"
     gene            42515..43045
                     /locus_tag="PLG49_00795"
                     /pseudo
     CDS             42515..43045
                     /locus_tag="PLG49_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            43845..44351
                     /locus_tag="PLG49_00800"
     CDS             43845..44351
                     /locus_tag="PLG49_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15877.1"
                     /translation="MTFIVTLIPQNIYAANSIESVLTEETDLEKDSDADHLTGDFEIK
                     FGRNPYKIDVYIDVYRNFIIDLFEFAEQVYSSKVDTDGDGLPDGYELLYLGTNPNSKD
                     SDNDGILDRDEDLDEDGLLDGDEVKLGLDPLKAYTYESILDSEYKIKQHLSEEIFNNI
                     KILFLQKR"
     gene            complement(44627..44866)
                     /locus_tag="PLG49_00805"
     CDS             complement(44627..44866)
                     /locus_tag="PLG49_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15878.1"
                     /translation="MKKILIKIIVFLIILILLIPVPIYVKDGGTIVYQAILYSVTNYH
                     SLYPDDTNEKDHKILTGIEIKILGFTIYKNTRFES"
     gene            complement(44936..45178)
                     /locus_tag="PLG49_00810"
     CDS             complement(44936..45178)
                     /locus_tag="PLG49_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15879.1"
                     /translation="MKKKWITIIAILIALILLIPQKSYLNDGGTVIYDAILYRVTNYH
                     SMYPDENDESQIRYLKGIEVKILGFTVYNNVKFEKD"
     gene            complement(45385..46020)
                     /gene="deoC"
                     /locus_tag="PLG49_00815"
     CDS             complement(45385..46020)
                     /gene="deoC"
                     /locus_tag="PLG49_00815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ15880.1"
                     /translation="MNIAKKIDHTVLKADATVETVKKYCQEAKEYGFASVCVNSCHVP
                     LVAQELKGSGVKTCCVVGFPLGAMLTSAKAFEAAECVKAGAEEVDMVINIGAVKDKNW
                     DLVLEDIKAVVEASKPAIVKVIIETCLLTDEEKVKVCELSKEAGAAFVKTSTGFSTGG
                     ATVEDVKLMKQVVGDELEVKASGGIRTAEFAKELLEAGADRIGASNGIALL"
     gene            46278..47330
                     /locus_tag="PLG49_00820"
     CDS             46278..47330
                     /locus_tag="PLG49_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ15881.1"
                     /translation="MNIYDIAREAGVSPATVSRVINGNKNVREETRKKVLKVIENNNY
                     APSSVARNLSIGESQNIAFIVPDVENPFFSQILQGITDKASDSNYNVFMFGTKEDPER
                     EHRILESLKGELIKGLIIIPVSEIDEETAFRLTNFEREGIPVVLIDRDIKGSNFDGVF
                     SEDIQGAYEAVECLIQEGHKKIAMITGPETSRPGHDRLRGYKMALTNYNIEINEDYII
                     NGNFIEEDAYKAMKTLMELKEPPTAVFSSNNMTTLGCLRYLKEHGLKLVEDISLIGFD
                     DIKELQYTDINLTVVARPVYEMGQEVMELLERRFSYKKEGDRKKMIVRRNYVKTWLIK
                     RGSEKYKKFQEGNVNE"
     gene            47323..48246
                     /gene="rbsK"
                     /locus_tag="PLG49_00825"
     CDS             47323..48246
                     /gene="rbsK"
                     /locus_tag="PLG49_00825"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOQ15882.1"
                     /translation="MNKVAVFGSFVVDLMARSPHLPVPGETVKGSMFKMGPGGKGFNQ
                     CVAAHKAGADVVMITKLGRDNFANVALDTMDELNMPKDHILFSDMTETGIALILVDEN
                     TSQNEIIIVPGACNTITEEEVKGIESVIKESEYVLLQLEVNQNANEMVVDMANQHGCK
                     VIVNTAPYVPISDSFLSKVYMVTPNEVEAESMTGIAVKDLESGKQAAKFFHDKGIEIV
                     VITLGSRGVFVSYDGKAEIIPAFKVNAVDTTGAGDAFNGGLLAALAEGKDIWEAVRFA
                     NALAAISVQRLGTTPSMPERWEIDAFLEAHN"
     gene            48261..48704
                     /locus_tag="PLG49_00830"
     CDS             48261..48704
                     /locus_tag="PLG49_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOQ15883.1"
                     /translation="MLKGIPTQISSDLLKALADMGHGDLIIIADDFYPPKTKTPNGIS
                     IQAKGNTAPEMIDAILQLFPLDVDYCAYPVEYMVPDKDAGISIERPKVWDDVIEVVKK
                     HGYDAKIVGEIERTKFYEKAAKAYVTVCTSERQPYGCFILQKGVM"
     gene            48754..49830
                     /locus_tag="PLG49_00835"
     CDS             48754..49830
                     /locus_tag="PLG49_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOQ15884.1"
                     /translation="MKMKKILASLLMGVMTISLAACGGTKTSTDSSNNSSATASSDDK
                     TFEIIYLTPSTASQFWTHVGIGIENAMKDLEEKEGIKVNYTIVGPAEESQTEEYVTAF
                     EKAIAKNPDAIVTATLAIDATVPKAKEATEKGIVLNFVNCGLGVGDDGAYQDYYNQFY
                     YCSNDTIGEMAAQAFLEAMEKKGIEKGVVGINMNVENEALNHRIQSFRDYMAANAPQL
                     TLTDTYYNGNVVETAQANAENIISTYGSELIGMYSGNNITGDGVCLAVESAGIGSKIV
                     SVAVDSDDIEINALKNGNLDAIIVQDAYAQGYKVMENAILTLLNGSNPESSKQVNCPP
                     VIVTKDNMDSEEMLNLLDPTRLKK"
     gene            49941..51449
                     /locus_tag="PLG49_00840"
     CDS             49941..51449
                     /locus_tag="PLG49_00840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOQ15885.1"
                     /translation="MQETILKIENISKFYPGVKALNKVSFELKKGEVRALVGENGAGK
                     STLIKCIMGVEKPDEGRILMNYNGEWICNTNALEAQRHGVFANYQHVNIAPDLSIAEN
                     YFLGQQPKTQLGFVDWKKMYDDSKKIIDKFQMKVNPTDKIRNLPIAMQAMVTISKISV
                     NEDIRIVIFDEPTALLENEKVEILFKFIEELKQRGVSIIYVSHRLEEIMEICDSVTIL
                     KDGTYVDTKPIEEVTKDSLISMMVGREMTDIYNIKHVEPGEELLRIENFSDSRHFKDI
                     NFTLHKGEILGFAGLVGAGRSEIMRAICGVEKRLTGDVYVRGKKVNIKDPSDAMKHGI
                     SFLTEDRREDGLALILPIKINVNMNSYDMISKRGVISLKKETERAEDYSKRVNVKTPS
                     IMQLAGNLSGGNQQKVVIAKLLCRDPDILIFDEPTVGIDVGAKEEIYKIIEKLIAQGK
                     GVILISSYLPEVMGLADRLIVMSQGKIRAEYGKEELKNLKEEDVLRKASIED"
     gene            51463..52434
                     /locus_tag="PLG49_00845"
     CDS             51463..52434
                     /locus_tag="PLG49_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ15886.1"
                     /translation="MSNTKSAKNSYKNLFQQTEFTLGVIIVLLFVIASVFTDNFFSMY
                     NITNLMKQCAITGVLAVAQTFIIITGGIDISGGAITGLSCMVLALLQRDTGMNFWLTL
                     VIAIIVSIAAGFINGIIVHDFKVPPMIATLGMSTIIRGIIKIISNALTVSGLDERILN
                     MGNSSIFGIIPTLAIIWLLVALAIFLVLKYTIFGRNLYVIGSGINVAKLSGIKVRKMF
                     LSVYALAGLLYGIAGIMLAARVQSALPTGGEGYDMSAIAAAVIGGASLAGGRGAIAGT
                     LLGTILMVLINNAGVQFGLNTHVLEITSGVLIVFAVTMDMLKNRKKA"
     gene            52793..53608
                     /locus_tag="PLG49_00850"
     CDS             52793..53608
                     /locus_tag="PLG49_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15887.1"
                     /translation="MGYDITYHPIKLEQMEEWYFSRLDELKQGDYSKTWEMAQKANMH
                     EFYIDKYLDVMKEAVNIEPDLTFEITHGFNIAVVQGFFLPHYYVRGTAFSFLIEEHPQ
                     MAKYITSWQDIIPQWIKNPIAGGIVENYCGGVFISPEQVHKLLSDYEKDIQIKNLIDN
                     FFGEYLPVFIKALNYSLENGLGLLEATDVVVPNVIDLNKTESYSNLFNCDPEGALIYR
                     EIAMEQIAQALKESGDNRDVNEALKDMAFMISERDEDGNVEDYRIYEHPEKDS"
     gene            53959..54498
                     /locus_tag="PLG49_00855"
     CDS             53959..54498
                     /locus_tag="PLG49_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOQ15888.1"
                     /translation="MSKIAVVYKSKYGSSKKYAEWIADELKAELFEHSKIKKDDLLKY
                     DTIIYGGGLYAGSISGISLITKNFDKLKDKNLIVFTVGTAQTDNTEVFKPIIEKNCSE
                     EVRRAVKFFHFRGSMDYKNMTLTHKMMMKMLKLVLEKKKEEGLSKEDKLFLETFGQKV
                     DFIDKAYISPLIEYVNALS"
     gene            54593..57994
                     /locus_tag="PLG49_00860"
     CDS             54593..57994
                     /locus_tag="PLG49_00860"
                     /inference="COORDINATES: protein motif:HMM:NF040034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6240 domain-containing protein"
                     /protein_id="HOQ15889.1"
                     /translation="MIGQVQSKELILDQNMLDLKFSMSLDWEQGELLEGDIISYGENK
                     VTVEIEGVKLDIKSSEKIGEVGDKVKFLVEKVTENELVLKALTDNQEIRPIKQYDRIQ
                     SVLLKEVIPMTEQNIELAKKMLDSGIQVEKEIFEKVAQAKRQIEQIINTMTREEVREL
                     LESPYDAEKISIDIISRFLCEKGKFSKAISSEELEKELNQYVGEKTEKVKVAMKALLE
                     KELPVTRKNIDSLVLVQEKLEAIKNMDDKAIEQVVKRKLPYTLNHLYAAIFSAGEEEI
                     QIESKLPYGDAGLINTGEHEFSQDEIIKLLKHQEMPIDEEHIEAANFLIKHKLEVNKE
                     NLEAVILLKHEIKKLDEKIVLDKALKFLKLNSNPGKLELFEDIYKKDKSLTENEIKQL
                     VRDIKVMDDEALKRVIRKNLSINLRNLQKELNSIAEEKNVDEESPLELEKGSQFINAK
                     RQLEEVRLKMTLEAALKLNEKIKIDTVPLKELVEELKSLEQEYYREALHKAGAESSKE
                     NLEQLEALYNKLETINKSGRMVLAKVVKNEIPPVIDDLYKQTLQEKEVITKYEQMETR
                     VEPRLGDVIRKIEKQIAPLLKEQGIEPTEENIRCARILIQNNMEVNEENILQVRVLDE
                     KVNFISKNLHPIIATNMIKEGFRPDFIPIDEVIDYIEVFDELLGKDPKEALGQFILEL
                     DEKGALSPQEREGLIGIYRMLHVIEKSNGKVIGWLMKNNIPLTLNNLVEAAKYLDRTR
                     GVKQNMDVLVDDDFGFSEKVIYHEKSIRAQLEKAFGEKVTDSSRFLNFDSISKETTEQ
                     LLDSSSLFKLDYYELILDEFVRNMSSEKFIEWLSKNPDLYEMDLESLYEEYKKEDLIK
                     EGNLSKTPVKEETVEEFTKTLQVIKKVNQETLIFMEKNQIPMNLKNLQLVSGLLENPH
                     KLGEEIESFSNLLKETDYKDELHQAIREINEKLKQGMDPEEAQDELKEKIKEIKGQLL
                     ESLLPVKQTVWKTGNDIEKMIDIGKQLQKQDGFYQIPVIINGKAANMNVYLLKDRDKG
                     ALAKKESISAYVAIETEHLGQVRMVIHMDEKNVDFKISGESKEVTEYLKSKEHLFRAS
                     IENIGYKVLRSQFKEELKELVQSSKGTSLVVNKKKHLDSDFEMVV"
     gene            58005..58172
                     /locus_tag="PLG49_00865"
     CDS             58005..58172
                     /locus_tag="PLG49_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15890.1"
                     /translation="MEEKENKKIPSSLLEVVLEMISTIDEIDKEKGNQEKTLKDYRNP
                     SINKLEKIIKR"
     gene            58183..>59286
                     /locus_tag="PLG49_00870"
     CDS             58183..>59286
                     /locus_tag="PLG49_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15891.1"
                     /translation="MRINHNIPALRALHQLNKTNTTLDKTMQRLSSGLRINCAADDAA
                     GLAIAQKMDTQVRGLHQANRNAMDGISLIQTAEGALNEVHAMLQRIRELAVQVSNGTY
                     DATDRRAVNDEVVQLQNEIQRISEHIEFNERKLLNGEIDRRAFPDNEGVVKIISLSDT
                     VSPGSYKIEVTADATKATIAGDSGKINAGGKISDAQVGTINLNGEEVRIEKGDTADVV
                     FNKLRDLAETIGANLIAYADNDGSPDYASGAQQYNFDNMHLVFEMKEYGSEYKLDLNA
                     SNSALLSALGLSTTVQQGTDAQAKITSTADGFSATATVSTKGNVITVTDINGFEMMFE
                     AQQGVAALTDSTANVKTANVKVLDAGPLDLQIGA"
     assembly_gap    59287..59386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <59387..59806
                     /locus_tag="PLG49_00875"
     CDS             <59387..59806
                     /locus_tag="PLG49_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ15892.1"
                     /translation="ANVKVLDAGPLDLQIGANEGQFMEIRIQNLSPQALGIDKINLST
                     SDGAQKAITVVDKAINMISSVRSKLGAYQNRLEHTVANLSVAAENMTASLSRIQDADM
                     AAEMSEYTQKNVIAQAGVAMLAQANQRPQQILQLLQG"
     gene            59821..60282
                     /locus_tag="PLG49_00880"
     CDS             59821..60282
                     /locus_tag="PLG49_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOQ15893.1"
                     /translation="MENRTAQIANASPGQLVVITYELILEALQEAQDQVPVKDEKKFK
                     RTIDRAQKLLKELMVSLDLSYEISYDLMSLYSYINKTIIKGYVSQKKEPLMEASKLLN
                     TLLEGWKQAVAAEGDNAPVIENAQQIYAGLTYGKGNLNESVIEDANRGFKA"
     gene            60400..60684
                     /locus_tag="PLG49_00885"
     CDS             60400..60684
                     /locus_tag="PLG49_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HOQ15894.1"
                     /translation="MNFNIETEKRGFFPFRFKSKQEEGLKEAEELKEELHKIKKELDL
                     AYNNFEYATEKELIDSCIYKMMALQIKYKYFLDKVKELDASFAKEWEQTS"
     gene            60712..60972
                     /locus_tag="PLG49_00890"
     CDS             60712..60972
                     /locus_tag="PLG49_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOQ15895.1"
                     /translation="MGSNEFLMTLIIIGSVILVLMIYPKPLKILLRIIIQALGGIAGL
                     YIFNFVLSPLGWFVGINWTTILIIGILGIPGLIFLYLLNLIL"
BASE COUNT        21841 a         8816 c        11142 g        19093 t
ORIGIN      
        1 gcgacaaaca ttgtaacaaa actattctag tttgtcaact aactttattt tggaaaaaat
       61 aaattggcgg agaaggaggg atttgaaccc tcgcaccgcg attaacggtc tatgccctta
      121 gcaggggcac ctcttcagcc acttgagtac ttctcctgaa tcaaacgcaa atgtaattat
      181 actaaaagca aagacaaaag tcaacttaat ttttaatgta aatatatgta aaatagtact
      241 attgctggtt gtatctttat tttcttaatc cgatatatga aatagcttgt cttcctaact
      301 gattccagga ttctttaaat ctttctttat taactttaat atttttctga ttgtaaaaag
      361 gatcatttaa ataaatattg ttttcgtcat atcctgtaat aacaactgca tgtagtttat
      421 atgttatttc aactggtcca tttggagtca tccagatttc aaattcactg ttatctaatt
      481 ttacatacat ggtatttgta attgcccaaa ccggataacc ccttgaaata aaatgaaaca
      541 agtcttcaaa ttcacatccc gttaaattga ttgtttcatc tggaagatat tctttcataa
      601 gttcttcaac aggaccatta aagacaccta aacctggtgc ttttaaggaa tacatgtctc
      661 caacaaaacc atcgttggga tttccaaaat aaactctgtt tccctttata ctaaaaggtg
      721 tttcatcttt tttaattttt ttagctaatt caattttatc aacttctatg ccagcataat
      781 taagaagcat tgccaggctg gttacttcac aacctcttgg caattcagga tattgcaata
      841 taacaggaac atcaagcaaa acattatttt ggagtttttc tgttttagac cagattatat
      901 tgccattgtt tttaaaatag ataacagaat tttctttttc tcttgcaaca aaaactgcat
      961 ctgcaaatga atcataatca ttaataaaga ggtcaccgtc aaataagata aatggtttaa
     1021 aattattata aatccattta tcactatttg acgctttaac ataggtatac ttgaattttt
     1081 tactgtactc tattgcctct tctttagttt taaaagattt gaagttttta tttttataat
     1141 agacttcaaa aactgttggt tcctggatat ttgctgaacg atcattttga atcattttac
     1201 ttgcctggct tatttctttt aattgttttg aatatacaat aagataagcc gttactaaac
     1261 ccaaaacaaa tatccaaaaa acaatagctt tctttttcat ttcaaatatc ctcttttagc
     1321 aataatgatt tattcttcaa caacctttgc tacagcaaca actttttcat cttcagaaag
     1381 attcatgagt ttaactccct gagtaaccct gcctgtcttt gaaatctgag agacatgcat
     1441 tcttataatg attccttctg aagtaatcat cataatttct tcatcttcag aaaccatttt
     1501 aataccaatg attttacccg ttttttctgt gagacggtag cacttaacgc cttttcctcc
     1561 tcttctttgt aacttgaatt cattgatatc tgttcttttt cctaaacctt tttcagtaac
     1621 aaagaggatt tctttacctt ctgaaattaa tcctgcacca acgatttcat caccttcatt
     1681 taaattcatg gctctaacgc cagtggcatt tcttccaata gctctgatct cttttcctga
     1741 aataagtatt ccttgtccaa atttagttcc aatgaaaatt tcatcttcat cagcaatatt
     1801 tattacctgg attaattcat cattttctct taaattaata gcatttaatc cagattttct
     1861 aatgttctca tattcattaa tactggtctt tttaataata ccatttttag taagcattaa
     1921 taaatagctt ccctctttat actctttaac cggaataata gctgttattt ttttatctcc
     1981 atcaatttga agcaaattaa caatagcaac tcccctggct gttctggaag cctcaggaat
     2041 ttcataagtc tttaaacgat atacctttcc tttattggta aagaagagga tatagctatg
     2101 agtagaagtc acaaataatt cttccaggat gtcttcttct cttgtctgca tacctataat
     2161 tcctcttccc cctctatttt gacttctgta agtatttaaa ggaagtcttt taatataacc
     2221 cagacgagtc agtgtaacca cgttttgctc atcagaaatc aaatcttcta tgtcaatttc
     2281 cccatcatca tgggcaaatt ttgtttttct atcatcccca tatttttgca ggatgattaa
     2341 taattcttct ttgataaccc catataatct tttttcactg gccaaaattg ctttcaattc
     2401 tttgatggtt tcctgaagtt ctctatattc ttcatcaatt ttttctcttt ccagaccagt
     2461 caaggttcta agtctcatgt cgacaatagc ttgagcctgt tgttctgaaa gagcaaaaga
     2521 ttctattaat ctttgttttg cttccggcgt gttctgagaa cttctaatga tagatattac
     2581 ttcatcaata tgatcaaggg ctatttttaa accttctaaa atatgggatc tttcttctgc
     2641 cttccttaaa tcaaactggg tccttcttgt aacgacatct ttttgatgtt ctaaataatg
     2701 aatgagcatg tcttttagat tcagaacctt aggttcatta ttaacgagag ctaatatgtt
     2761 aacactaaag atatcctgaa gctgagtata tttataaagc tggtttaaaa caacattggc
     2821 attgtaatcc tttttaattt ctatcacaat ccgcatacct tcacggttgg attcatccct
     2881 tatatcagat ataccatcta ttcttttttc ttttacgagt tctgctattt tttctattaa
     2941 ttttgcctta ttaaccatat aagggatttc tgttacaata attctttgtt tgttattgga
     3001 catggattca atttcagaaa ccgctcttac ttttatcttt ccttttccgg ttctgtaagc
     3061 ctcttcaata cctcttcttc caagaataac tgctcctgtt ggaaaatcag gtccctttat
     3121 aataggcaat aattcttcaa tgtcagtttc tctattttct tctacataat tatcaataat
     3181 ttttacaacc ccgttaatga cttccgttaa attatgagga gggatattgg ttgccatccc
     3241 aacagcaatc ccagaagtcc cgttaacaag taaattagga aatctggaag gtaaaactgc
     3301 tggttctttt aaagattcgt caaagtttgg attgtaatca attgtatctt tttcaatatc
     3361 tgctaataat tctaaagcaa ttttacttaa tcttgcttcc gtatatctca ttgcagccgg
     3421 actgtctcca tcaacagaac caaaattacc atgcccgtct actaaaggat atctaattga
     3481 aaaatcttgc gccaatctaa ccattgcatc ataaatggat gaatcaccgt gggggtgata
     3541 tttacccatg gtttcaccga cgatacgggc agatttttta tgaggcttgt ctggagttaa
     3601 acccaattca ttcatagcat ataaaattct tctgtgaact ggttttaatc catcccgtac
     3661 gtcgggcaat gctcttgata caataacgct catggcataa tctatataag actttctcat
     3721 ttcttcttca atttctacag gtataatcct ttttttcagt tccttttctt ccatgactga
     3781 cctccctgat taatccgcta gatatcaaga tttttaacgg tttttgcata ttcccggata
     3841 aactctcttc ttggttctac tttatctccc attaaagctg taaaaacttc atctgcagct
     3901 gcagcatcat ttaaatcaat ctttaaaagt atacggcttt caggatccat cgttgtttcc
     3961 caaagttgtt caggattcat ttcccctaaa cctttatatc tttgcatttt tatattgtcc
     4021 cggccaattt cactaaagag tttttctaat tccttatcgt tatagacata ataaaccttc
     4081 ttattctttt ctactttata aaggggcggt tgcgcaatgt aaacatgacc tttttcgatt
     4141 aattctctta aatacctata aataaaggtt aaaagcaagg ttctgatatg tgccccgtca
     4201 acgtcagcat cggtcatgat aataattttg tcgtatctta atttttctat atcaaattct
     4261 tctccaatac cagtaccaaa agcagtaatc attgccctga tttcttcatt tccaagaatc
     4321 ttatccagtc ttgctttttc tacgtttaaa attttacctc taagaggcag gatagcttga
     4381 gtttgtctgg atctggccga ttttgcagaa ccaccagctg aatccccttc gacaataaag
     4441 atttcgcaaa gtttaggatc tctttcagaa caatcagcta attttccagg caagctattg
     4501 ctttctaatg cactttttct tctggttaaa tctcttgcct ttcttgctgc ttctcttgct
     4561 cttgctgcca tgataccttt ttgtaaaatg gtctttgcta tggttggatt ttgctctaag
     4621 aaatacatta attcttcaga aagaacagct tcaacaatac ctcttacctc actgtttccc
     4681 aacttggttt tggtttgtcc ttcaaattgt ggttctggta cctttacact aatgatagct
     4741 gttaaacctt ctcttacatc atctccggaa agatttttat ccgtttcttt aagtaaattt
     4801 gcttttctgc cataatcatt gatggttttt gttatggctg atttaaagcc gataagatgg
     4861 gttcctccct cagtagtatt aatattattt acaaagctaa agatattttc tacataagtg
     4921 tcattatgtt gaaaagcaat ttctacttca acattatctt ttcttccttc gcaataaaaa
     4981 atttcatcgt acaatacatc tttatgttta ttcagatggg caacaaattc ttttattcca
     5041 ccttcataat gaaagacttt ttctttttta ttttcttctc ttaaatctgt caaagtaata
     5101 ttaatacctt ttgttaaaaa ggccgtttct cttaatcttt gtcttaatat atcaaattga
     5161 aattctacct cttcaaaaat ttcatcatca ggtagaaagt gaaccaaagt acctctttta
     5221 tcagtctctc caactacctt aagcggttga actaatttgc ccctttcaaa aacttgctca
     5281 taaatttttc catctgtaaa gacctgaacc tttaaatatt tcgaaagcgc gtttacaaca
     5341 gcagctccta caccatgaag tcctcctgaa accttatagc caccaccacc aaattttcct
     5401 ccagcatgca aagtggaaaa aaccacttct acagcaggta ttttcttttg aggatggata
     5461 cctacaggta tacccctgcc gttatcaata actgtaatgg aattatcttt atttatcgtt
     5521 acttttattt catcacaata accagctaaa gcttcatcaa ttgcattgtc tactatttca
     5581 tataccaaat gatgaagtcc tcttgaagag gtacttccaa tatacatacc tggtcttttt
     5641 ctgacagctt ctaaaccctc taatacctgt atttgattct catcatagcc tggtaccata
     5701 tcaactcact cctttatatg tacaatgact taaaatccag tttgataatg agatattctt
     5761 ttttgtaaag caactgaaga aatcggagaa aaataaaata aagacttgcc gttttcttct
     5821 gtaataatga ccgattttat ttcttttgaa ataagcttat gtaaatcttc attttggact
     5881 atatttaaaa aactttgagc cgtttttgaa gtcattaatg attttatatc aaatattcca
     5941 acaatatcct taacccttat ttctgtatct gcaccaatat gcagaaacat actttttcct
     6001 ccttataaag tcaactatac ttattttatc ttatttatac tgatgttaca agaattactg
     6061 tccctgggct caacaaatcc attctcaatg taaaataaaa atcctttatg taattttttt
     6121 aaaataatat cttcaattcc cgtacaagtc agtatggttt gtatatcaga aatattagaa
     6181 agtaaatcag cttgtctgtt ttcatctaac tcagaaagaa catcatctaa aagcaaaatt
     6241 ggattatctt tgacctcatt tttaattaaa tcaatttcag ccagctttaa agataagata
     6301 acactccttt gctggccttg agatccataa gttctgacgt cattatgatt gattttaaaa
     6361 agtacatcat ccctatgagg accaatagaa gttattcctg ttttaatatc tttttctaaa
     6421 aaacttttca acttgtcttt aaagttttct ttttcaacat taggaaaata ttctatctcc
     6481 aaatattctt tattagacgt aatatttcca tgaattacag aagcaatatg gcttatttta
     6541 tttataaaat gctttctcgt ttcaataatt ttttctccat aaaagactaa ttgctcatcc
     6601 caaacttcca gcattgcttc ctgaccttta tcataattaa tgtttttcag cagatggttt
     6661 ctttgtttta acactttata atattgctga agattatata aataaatttt atcgatttga
     6721 caaagttcaa tatcaatgaa tcttcttctt tccttaggac cgctttttat taattgtaaa
     6781 tcttcaggag aaaacataat aatatgcata attccaagca ggttacttaa cttttgtacc
     6841 ggaacttcat taataaaaac atatttctta ccatttttat ttaaatgcaa atctatttta
     6901 tcaagcctgt ctcctttatt aagaaataat ctgatatggg cttcttcttt atcccatttt
     6961 atggtttctt tttctcggga agtcctgtga gacctggcag tagcgcacat ataaatagct
     7021 tctaaaatat tggttttccc ctgggcattg tttccatata aaatatttaa tttggggtga
     7081 agtttaatat ttgtattatc ataatttctg aactgataaa ggtctatttc gcttatatac
     7141 attattccca ctccactaat tgattataat atactcattt ccatcaattt ctactttgtc
     7201 tccatttttt aattttttac ctctttgctt tacaatttcc ccattcactt ttatatcctt
     7261 ttctaaaata aaatactttg cttcagctcc agaagccaca atattaacat attttaaaaa
     7321 ttgatctaac tttatatatt cagtactgat tttaactttt tccaaataaa tcaccttttc
     7381 attttaaaat ttaactgata aaaataaaaa gtgcgaatat caccgcacct tttattataa
     7441 catatttaaa tatttaatat aataaatata attatccatt taaacggatt ggtaaaatga
     7501 gatatttata atcttcatta tcaacaggtt ttataataca aggatttaag gaagatataa
     7561 actgaatttt aatttcttca tcatcaataa ccctcagggc atcaattaaa tattttggat
     7621 taaatgcaat ggttaaatct tttccttctg tatccactgg cagttcttct tttgcggtac
     7681 ctaattcagt atttgatgta ataatcattt tatcgccagt gatatctatt tttataggat
     7741 tctttcttcc ttctcttgta attaatgcag ctctttcaat actcattaac aattccctgc
     7801 gattaacctg tatcatcgtc tcgcaatctt caaaaaagct attttcatat tttaagaatt
     7861 ctccttctaa caaacgggag acaaccatac tatcgcctaa gtcaaataaa atatgcttat
     7921 ttgtaaaata aatataaacg tcttcttcat cagaagataa tattttattg atttccccta
     7981 atgtttttcc gggaacgatt acttcttgtt cttccctttc aaccgataaa ggagcttgtc
     8041 tgtaagaaac cctgtatcca tcaacagaaa ccaaacgcag atgattatct cttatttgaa
     8101 ataattctcc tgtaaggaca ggtcttgttt cttctgttgc tatagaaaaa atggtttgcc
     8161 tgatcatatc ttttaaaacc gcttgtttta atacaaaatg cttatctttt tccactttgg
     8221 gtagttctgg aaaattatct ccgttttgtc cggaaatttt aaattctgaa ttttcgcact
     8281 taatggtggt catgttattg gaatctacac tgattaaaac ttcaccatcc ggaagctttc
     8341 ttacaatttc agaaaatatt tttgcttcaa gggcaataga acctgttttt tcaacatttg
     8401 ctattacctt actttctata cctatttcta aatcatttcc agttaattta agataatttc
     8461 cttcagcttc taataaaata cactctaaaa taggtaaagt tgttctgttt gatacagctt
     8521 ttgaaacaat atttactcca tttaataaat caacatttga acaagtcaat cgcattgtgg
     8581 ataactcctt tcctgtcaac tatattaatt attaaaaaag gattgagtag tcatagtagt
     8641 agtagctgtt gaaatgtgga aaacctttaa agatacgata aaataaaaaa aataaagttt
     8701 gataaagttg tggataataa aaataaaata aaaatattat ccactatatc aacattcaat
     8761 catgaaaaga aaaattatca acatcttgtc cacttacttt taacagctca atgtgaataa
     8821 ttatttacca gtaattctct tttctaaatc ttctatggtt tgcttgaggt caaaatcctg
     8881 taaaatttct ttttcaattt tttcatagcc gtgaataacg gttgtatggt ctcttccgcc
     8941 aaatttttct cctattttag gaagagaaag gtcagttagt tttctgcaaa gatacatggc
     9001 tatttgtctc ggataagcaa tatttctggt cctttttgga gaccttaaat cctctggttt
     9061 tatattataa taagaagcta cgacttcttg tatgagttcg gttgtaatcg tttttggctt
     9121 gtccgcactc attaagtctt taagtgcttc acttgccaga tctacagtga tttctttatt
     9181 tgccagggtt gaataagcaa tgattctgtt taatgcacct tcaagttcac ggatatttga
     9241 aacaatagat tttgcaatga ataaaataac atcattgggt acgattaatt tttctatttc
     9301 tgcttttttt cttaagatgg caattcttgt ttctaaatca gggggttgta tatcagcaat
     9361 taaaccccat tcaaatcttg aacgtaagcg gtcttccagg gtcttgattt cttttggagg
     9421 ccggtcactg gaaataataa tttgtttatt tgcttcatat aaggtattga atgtatggaa
     9481 aaattcttct tctgttcttt ctttaccagc aataaattga atgtcgtcaa ttaataaaac
     9541 atcaatattt ctgtatttat ttctaaaggc ttggttttta tcatccttaa tagaattaat
     9601 taattcatta gtaaattttt ctgaagatgc ataaagaacc ttaacattag gattttgaga
     9661 taaaatataa tgtccaatag aatgcatcag gtgggttttt cctaaaccta caccaccata
     9721 aaggaaaaga gggttatatg cttttgcagg agcttcagca acggccaagg cagcagcgtg
     9781 agccattcta ttactatttc ctattacaaa agtatcaaaa acatattttg gatttaggtt
     9841 gctttgatta tccagtggat ctgtatcatt atcttttata ctgttgtatt tttgactttg
     9901 attgaattca ttcgaagtaa caatttgaat ttcaaattcg tggtgagtta cttgaatgat
     9961 ggcattttgg ataagacgga tatatctgtt ttttaagata tcttttgaaa aatcgtctat
    10021 agtttcgata aataaagtgt tatctctaac atcgacaggt tttagatttt taaaccaagt
    10081 ttgaaaacta accgtagaag tttcagactc taaaatttcc agtatgcttg gccattgttt
    10141 tgtaatttga tttagcatta gagattcctc ctaaaatttc ctcctgatat tcaataaaaa
    10201 agtcagatag atattaacat aatatccaca gatgtgaatt ttgttaaaaa aattttaaca
    10261 gcatgtgaat aagagttgat tatttgttga aaggaaaaaa aatattaaca gtttgttgaa
    10321 aagttattaa cagaaaaaat acattattaa tgaagaaatc ggaaaaataa ggtttatgca
    10381 caaacttatc cacagaatgt gcataagttt atgcctttat ataattatta acagtttatc
    10441 ttaatgataa caaaaaaatt gtggataagc aataataaaa aaattatcca caggctgcaa
    10501 atgtgatata aatgtggatt tctttataca gaaataggtt cttgacgaac aaatatatat
    10561 ttaatataat tgtgttagtg cttacaaata aaataaaatg tgggctaagt cgacagtttg
    10621 ctgatttgta tagacgacaa aggaggtgct gaagaatgaa aagaactttt cagccaaaga
    10681 aaagacaaag acaaaaagaa catggttttc gtaaaagaat gaaaacagca agtggcagag
    10741 ccattttagc aagaagaaga gcacgaggta gaaaaagatt atcagcataa acaaggccgc
    10801 attagtgtgg ccttgttgta ctatatgctt tttcctccaa cttaagactg tttttaggag
    10861 gagatttatg aaatttactg actctttaaa aaaaaattat caatttcgta tcgtatataa
    10921 taagggaatt tccagtgcta atcgtttatt agttatgtat ttattaaaaa atggaaaaga
    10981 tattaatagg ttaggtattt cggttagtaa aaaagtcggg aatagtgtaa aaagaaacag
    11041 aataacgcgt ttgatcaggg aaagttacag attaatagaa gacaatatag acagaggttg
    11101 ggatatcgtt attattgcca gattaccatg taatggttca aattttaaag agatttcaga
    11161 tgctctgatt catttattaa agaaaaataa cctttatatc gcttagcttt actaaggaag
    11221 gatgaatatt tttttgaaaa aaattctttt attcatgatt tcattttata gaaaatttat
    11281 ttctccttta aaaccagctg tttgtcgttt ttatccaact tgttctgcat attcttatca
    11341 ggcaatcgaa aaatacggag taggtaaagg tttatatctt tctgtaagaa gaattttaaa
    11401 atgtcatcct tttcatcctg gtggatacga tcctgtcccg taataaattt tgaggaggaa
    11461 atagtttgat agatgcatta gcaaaaattt taggagttgt gtttaatttt atatttgatt
    11521 tggtatataa ggttactccc gttggttctc ttggaataac gattatttta tttactattt
    11581 tagtaagatt gttaatgatt cctttagtag caaaacagca atcttcattg aaaagaatgc
    11641 aggagattca gcctgagtta caaaaaattc aggataaata taaaaataag caagatcctg
    11701 aatctcaaag acaaatgagt atagaattaa atgaatttta taggaagcat aatgtaagtc
    11761 cttttagtgg ttgtttgctt ttatttattc aaatgccaat ttttattgct ttgtggaggg
    11821 tacttcaaaa agcgggtaca tatatatcta aacttcatga cttatattat aatttagcaa
    11881 gtcagattgt ttcagcagat ggttaccagg aaattgtaaa aaatatttta tcaggaaata
    11941 aaagaataaa acttaatgat gaaattttag cttcagctga aggattacaa agtattttat
    12001 ctcaagtatc aaccagtgaa tggaataaat tatctgaaac tttgggaacg atttcaaaca
    12061 cactgattga gcaaaaaaac agtatagaat atttctttgg tattagttta gtagatagtc
    12121 ctgcagattt aataaaacaa ggattaaata taccagcttt aattgttgtg attttattcc
    12181 ctattatggc tgctttaacg acttttcttt cttctaaatt attatcaatg aataataaga
    12241 ataataagca gaagcaaaat gaccagaccg aacaaattca aaaatcaatg aatcttgttt
    12301 ttcctgttat aactggaatg atgacttatt ctttgcctgc cggtttaggt atatattgga
    12361 ttacaagcaa catcatacaa atcatccagc agtatgttat taataaatca atggataaat
    12421 cgggggaagg tgaataattt atggattttg ttgaaaagga aagtaggaca gtagaagacg
    12481 ccattaccga aggcttgatt gaactgggaa caacaagaaa aaatgttgaa atcgagatat
    12541 tggataaggg ctcaagaggt ttgtttggtt taattggtgc aaaaatggca aaagtaagat
    12601 taacaaaaaa ggttaatcca gttgatcttg cagtaaactt cttaaaagat gtattcgata
    12661 aaatgaattt agacgcggaa attatcgttg aagaaaaaga agaaggcaat tatgtattta
    12721 atattcaagg agacgatatg ggtatattaa ttggtaagag aggtcagact ttagattctt
    12781 ttcaatattt agctaatatt gttgccaata agaacaaaaa caatcacatt catattactt
    12841 tagacacaga aaattataga gaaagaagaa aagaaacttt agaaaccctg gcaaagaatc
    12901 tggcaaagaa agtaaaaatg acaaagaaga gtattgtact tgaaccaatg accccctatg
    12961 aaagaagaat cattcatttt gcacttcaaa atgataaaca tattaagact catagtgaag
    13021 gtgaagaacc ttacagaaag gttattatca gcccggttat tgataagtaa aacccagtat
    13081 aaactgggtt ttttaaaatt cagatttaat ggaggtcaaa atatatgatt gatgatacca
    13141 ttgctgctat ttcaactcct ccaggggtgg ggggaattgg aattataaga gtaagcggca
    13201 gggatgcttt ttctattgtt aattcaatat tcagaagtaa aaaagttaaa gatttatctt
    13261 cagtaaaaag tcatacgatc cattatggcc atattgttga tggagattcg cttattgatg
    13321 aagtattggt ttcagtcatg aaagcaccaa acacttatac aaaagaagat gtggttgaaa
    13381 ttaattgtca tggtggaatg gtttccgtta aaaaggtgtt agagcaggta ttaaaaaatg
    13441 gggccagatt agcagaacct ggagaattta caaaaagggc ttttttaaat ggtagaatag
    13501 atttatctca ggctgaggca gttatagata ttattaattc aaaaacggaa aattcattaa
    13561 aactggcagt gaatcaatta gaaggaagat tatcaaaaga aataaagaaa atcatggata
    13621 agatcattga aatcatggct catatagaaa catctattga ttatccggaa tatgatttag
    13681 aagaaatcaa ctatgataat ttagaaaaca atataaaaga aataagaaaa gaaatccgtc
    13741 atttattaga gactgcagat tcggggaaaa ttatgagaga aggtgtgaag accgttatcg
    13801 taggaaaacc taatgtcggt aaatcttctc ttttaaatgc ccttttaagg gaacagcggg
    13861 ctattgtgac cagtatacca ggaacgacca gagatgtttt agaagaatac attaatattt
    13921 ctggtatacc ttttaagatt attgatactg ctggtataag agaaaccaat gacattgtag
    13981 aaaaaatagg tgttgaccgc tcaaaagaat tgttggaact ggcagattta attatattta
    14041 ttattgactt gtcggttcct ttgacagaag aagaccgata tataatggat ttagtaaagg
    14101 aaagaaaaac tttagtgatt ctaaataaaa cagataaaga aacggttatt aataagaaag
    14161 aaatatatga taaatttatc aataataaag tattggaaat gtctacaaaa aatcctgagg
    14221 acattgaaag attagaagaa actttaaaag aaatgtttga tgaagggcat tttaatattg
    14281 atgattcagc tgttatttca aatatgaggc ataaaaatgc tttaatcaag gcaaataata
    14341 gtttagaaga tgtcttaaaa agcatcagta tgaatatgcc tgtagatttt ctgtccatag
    14401 atttatccga tgcatataat tttttaggag aaattacagg agagactata gaagaagatt
    14461 taattaataa gatatttagt gaattttgct taggcaaata atccaaaaaa agagaagagg
    14521 taatggtatg ggctatgttg tttcagatat tgatgttgct gtgattggag gaggacatgc
    14581 aggctgtgaa gctgccctgg caacggcaag aatgggtcta aatacggttt tatttgctat
    14641 tagtttagac agtattgcca tgttgccatg taatccaagt attggaggaa cttctaaagg
    14701 tcatttggta agggaaattg atgctttggg tggcgagatg ggtaagaata tcgataaaac
    14761 ctttattcaa tccagaatgt taaacacagg aaagggcccc gctgttcact ctttaagggc
    14821 tcaggcagat aagcataatt attcaaagca aatgaaaatg actttagaaa gaacagaaaa
    14881 tctgcgtata aaacaagcag aaatcgtcaa gatacatgta gaaaataata aagttaaagc
    14941 agtagaaaca gctacgggag ctatttataa ctgtaaggca gcaattattg ccacaggaac
    15001 ttatttaaaa gcccgttgtc tggtcgggga tgtcattgaa tatacaggac ctaatggtct
    15061 gaaagcagct aataatctca gtgaaaattt aaaagaactg ggtattgaat tattccgttt
    15121 taaaacagga acacctgcca gagttgatag gagatcgatt gatttttcta aattagaaga
    15181 acagccaggt gatgagaaaa ttgttccttt ttcattttca accaatgcta aagatattca
    15241 aagagaacaa atttcttgct ggttaactta tacgagtgaa aaaacccatc agattattaa
    15301 ggaaaatctt cacagatcac ccctttatgc aggtgaaata aaaggggtag gagcccgtta
    15361 ttgtccttct attgaagata aggttgtccg ttttgctgat aaagaaagac atcaggtttt
    15421 ccttgaacca gaaggggaaa atacaaccga aatgtacgtg cagggtatgt cttcttctct
    15481 tcctgaagat gttcaaatta agatgttgcg taccttacca ggttttgaaa aatgtgaagt
    15541 catgcgggtg ggttatgcca ttgaatatga ttgtatcaat ccgattcaat taagacatac
    15601 cttagaattt aagaaaatag atggtctttt tagtgccgga caatttaacg gtacttctgg
    15661 ttatgaagaa gcagctgctc agggtattgt tgctggtatt aatgcagcct taaagatttt
    15721 aggaaaagaa cctctttata ttgacagatc agagggttat ataggtgttt tgattgatga
    15781 tttagtaaca aaaggaacta atgagcctta ccgaatgatg acttcaagag cagaatatcg
    15841 tttgctttta agacaagaca atgctgatct tcgcttaatg gaaaaaggct accgagttgg
    15901 cctggtttct aaagaaagat atgaaaaatt actggaaaag aaaagactca ttgatgaaga
    15961 aattaaccgc ctaaagaaaa cggttatagg agttaaccaa aaggttcagg attttcttaa
    16021 ggctcacaac agtacgccca taaagtcagg cattaccctt tcagaattaa taaaaagacc
    16081 tgaactggat tattataaaa ttagagaaat tgataaagat tatgtagatt tacctgaaga
    16141 tgttcaagaa caagttaata ttcaaattaa atatgaaggt tatattaaaa gacaaatgca
    16201 gcaagtagag caatttaaaa agctagaaaa aaaatatctc agagaagata ttgattatac
    16261 taaaattaaa ggacttagga ttgaagccgc acaaaaattg cagcaaataa aaccaagatc
    16321 gataggacaa gcatccagaa tatccggagt gtctccttca gacatatccg ttttactggt
    16381 ttatcttgaa caggtcagta gaaacaaaaa tacaaatcaa taggagtttt tatatggaac
    16441 taaaagatta cttaatagaa ggtagtaaac agttaggagt tcatttagaa gattatcaga
    16501 cagaacagtt ttttcaattt aaagatttat tattagaatg gaataacaag attaacttaa
    16561 cggctatagt tgatgacaag gaaattatta taaaacattt cctggattgt ctttctatag
    16621 gtcagttttt tgactgtcag actgcctgca atgttattga tgtgggtacg ggagcaggtt
    16681 ttcccggcat tcctcttaaa attgcttatc ctaatttaaa aactactctt ttagattcct
    16741 taaataagag gatcagtttc ttaaaagaag ttgttttgaa tcttcagctt aaagatgtca
    16801 cttgtattca tggacgtgct gaggagttag gacaaaataa ggaatacaga gagctttatg
    16861 atttatgtgt atccagggct gtggctcatt tatcggttct ttcagaatat acattaccct
    16921 ttattaaaac gggtgggaca ctgattgcca tgaaagggct taatgtggat gaagaattaa
    16981 atgaatctaa aaaagcaata gaagtactgg gtggagaaat catagatata aaagatgtag
    17041 ttattccctt tagtgatata aaacacagac ttattatgat aaaaaaagta cgacttactc
    17101 cgacaaaata tccaaggaaa gctggaaaac caggtagaaa cccgataaaa taatatattt
    17161 tatgtagaaa taagaagaaa attcaagtta aaaaaatttt tgctaagtaa attcatgtga
    17221 tataatacaa ataatgtata ttttttaaat gattttttga tatgggggga atagctatgc
    17281 aggcagaaaa gtctaaagaa ttaaaaattc aatggattcc tattgataaa attcgtccta
    17341 atccatatca accaagaaaa aattttgata gggctgcttt agatgaattg acgaattcga
    17401 ttaaagaata tggtgtcatg cagcctatca gtgtccgttt gattggcaaa tcaaattatg
    17461 agttagtggc aggagaaaga agacttcggg cttgtaaaaa tgcaggttta agtgaaattc
    17521 ctgctgtttt ggttgaggtt aacgataagg aaagtgcact attatccctt attgaaaatc
    17581 ttcaaaggga agacttaaat tttattgaag aatcagaagc ttattttaat ctaattcaag
    17641 attatggttt tactcaggaa gaattagcaa gaagtttagg taaaagtcaa tcgaccatag
    17701 ccaataagct aaggttgtta aagttatccc ctaccgttaa aaagattatt ttagaaaatg
    17761 atttatctga aaggcatgcc agagtattac tgaagttacc cgacgaagat atgcagcttg
    17821 aagtattaga aaaagtgatt attcatggtt taaacgtaaa aaatactgaa gaattggttg
    17881 aagaaatgat tgaaaggatt attcatgaat ctgaagaaaa aaaacagcag aaaatgaaaa
    17941 gagtcttaag agatatcagg ctttttacta atacaattaa tcaagccatc gatttaatta
    18001 aaaaatcagg ggtagaagct aagtatacca tggaagaaaa agaggatcat tacgaaatta
    18061 agattaagat acctatctat taaaaaggac tgtacacagc tttagtgtgt acagtccttt
    18121 tttattttaa taaaaatgtt tcacaaattt ggttaaaggt taaagcttct tcttcatggg
    18181 gcattgcttt agagttttta aagatataag tagaaacaga tgcttttact tttttccatt
    18241 cttcaagaag ggacaatggg actaaagtat tgtcttttcc ccataggatg agcacatctg
    18301 tcttcatttt actaaaggct tttttaacat ctatattcat atagcctcca ataaaagatg
    18361 caagagtata cctggaagaa ggacccttat catggctcat gccatgataa gtatctatca
    18421 gatcttttga aattaatttc ttagaattat aaagatattt ttgcatgaaa taataagtat
    18481 ataattttga agaaatcatt tgataaatgc ttgttcctat aaaaggcaat tcaatgaata
    18541 gtttcattaa cttgctcttt tgggtaggat aagaagccag tttattgatt cctgtaggag
    18601 cgatcaaaat cattttttta attaaggacg gattataaga agaagacatg atggcaaaag
    18661 aagctgcttg agaattggct attacataag ctggttcttt aataatgtct ttaataaaat
    18721 cattaattaa ttgtacatat aaaaaggcag tataagtcag tttaggttta gctgaaaggc
    18781 cataacctaa taaatcaatt gcatagaccc tgaagttttt tgaaagataa ggaatgtttt
    18841 ttctccattc acaggaagaa gccccattgc ctgtgctatg gattaataat aagggaggac
    18901 cagagccctg aaccctgtaa tgtattttcc caaacttcca gggataccaa tgataacttt
    18961 ttttgatttt attaaatttt aattttgaag ttaagaagat gagttgattc aataattgta
    19021 tggatagcaa tatcattgca aaatttaaaa gttttttctt attcttcatt ataagaatgc
    19081 gcccctttca ctccttttat tatattatct cttaaatagt aaaaaatgca aagaaaaagt
    19141 cgtaaattta aattaaacgg atattgtttt ttatggtata aggtataaca aaataaaaaa
    19201 gcataaaaat agctgataaa agaccagaat catttaataa aataacagaa agagaaatca
    19261 ggaaaaaaat attcttccaa agcttatcat aataattatc gttcttattt ttaaatagaa
    19321 ttagccactc taaaaatagt aagatattta gaggaggaat gattaaaaat aaaagaattt
    19381 cttttatttt agtgcaaaga attattaaga ctgtttgaaa ttcattattt tcaattaaat
    19441 tgataaatga agcaagatgg gtggaatatt tttgataatt atcaaactca ataaagtagt
    19501 tttgaaatat taaaaaaacc gcaatgaaaa caatagtata caaaaacttt ttataatttg
    19561 atagttcaaa aaatattgtt aaaataataa tagatataaa acagttaagc attcctccaa
    19621 tatttgcgcc gtataaggga gaaaatatta tgatataaat aaaaaatatt gcagctaaaa
    19681 aatacagaag ttttttgtca ggataaatat gattgatgta aatcattcca agtaaagaac
    19741 caataaagat tccgattaag tcattattcc agccatataa gcggttacca tgcagtatat
    19801 tattgaatcc tataggactt tgatacaaaa aaattttaga gaaactaaaa taaatcaaaa
    19861 atagtaaatt agaataccag ataattaaca ttgaactttt ataatcaaac tttttataat
    19921 atagaatagt accgggaatg aaagaaagca agatgattat taatgcttta tatataccat
    19981 ttaaagagtt aaatatattt aaacctgata cagaagacaa aaaaaaacgg aatgaaaata
    20041 aaatattgaa ataagatttt attttgctta gaaaaaacaa ataaagtaaa ctgtattaag
    20101 ataaaagtta tgagaatata gcggccataa tacaatgatg tatattgata atagattttt
    20161 tggatttttt cgtttgcttt ttttgtattt tgaacagaaa gagaaccact ttctaagtga
    20221 ttattgatga tattaattaa ttctgtatat ttaataatcc cttctctttt gcagaaatta
    20281 ctttgtagaa taccatttag atcagattca atgaataaag gattcatttg cttttctaaa
    20341 ttaacaataa ttaaacttgt atcatctttt ttaaaatttg ttatttgttc aaattgattg
    20401 atttttacct tttctaatat tattatagga cttattgaac tgtattcccg ttttatatat
    20461 tcgttaaaat tcattaaggt cacatcttgt tttcttttaa ggtcaaaata taagtcttta
    20521 aagtttttcc tgggataaac atgtaagaga ctatatactc ctttgttttt aagttgattg
    20581 aagttaaaat cttctatttc aacaattaga attttttctt ttaatttgga tttatcatat
    20641 gaaatagaat ttgagtcttc tttatgtttt aactgactta aacttataaa tagtatactg
    20701 agaaaagtgg tataaattaa aatttttttc ataaaattca cctttgtctt taatgtttaa
    20761 gaaagttata acagaggctt tatgtaattt gtaaatataa aactaagtaa gtaaaagatg
    20821 ctcatcatca ataagaaaag cgcataataa aatgaaatta aattttttga aggtggttga
    20881 tattttttaa aggcgcagat aaaaaataca atcgaaaaaa taatctgaaa catttttttc
    20941 accgtttaca tgttattaat ttattatagg attgtattat aggatatgag tatttatttt
    21001 ttttatccat tgataaaaaa atgtttaaaa aagaggattt taatatgaat taatcgaaat
    21061 atatacaaaa acgaatttat tgttatcata atatcaattt agctggggaa cggatggcga
    21121 tatgcatgaa gtacaatgaa atggacaaat tagatcaaat tttaaacagg accattgaaa
    21181 gcatcgaaaa aagtaagact gaaatgtatg aaatttatga aaaatcatat agagaatatt
    21241 taagtttaaa aaatgaatta agtgaattaa aaaagaaaat caggctttct acccgtttga
    21301 ttgagggcta tgaaaaacaa ttggcgcgaa gtaaagtaaa tttaatggag gttagtaaaa
    21361 attttgatca atcgacagaa gagaaaataa aagaagttta tgatgagacc gatcaattac
    21421 gcattgactt agcagttgaa aaagaaagag aaaagagttt aattaataaa agaaatgaat
    21481 tggaagttcg aataaaggca ttaaaagaaa ttgtaaacaa agccaataat ttaataaata
    21541 atgtgtctgt tgccatgaag tttttaacgg gagaccttaa aaacctttcc agccaaattg
    21601 gagatataaa agaaaaaaga aatatcggaa ttaaaatatt gattgcacaa gaagaagaaa
    21661 gaagaaggat tgcccgtgag attcatgatg gccctgccca atatatgtct aacttggtat
    21721 taaaggcgga atattgtatt aaacttctcg ataacaactt agatgaagca aaaatggagt
    21781 taggaaaatt aaaagagttg ttaaggagcg gtatccagga gttaagaggt attatttatg
    21841 atttaagacc tatgtcttta gatgatttag gtcttgttcc tacaatagaa cgttatatat
    21901 cgaaagttat ggaagaaaaa gatatcgtta taaaattatc agccagcgga agtaagcaaa
    21961 acatttcaaa tgttataaaa ttaacaattt acagactggc tcaggaagca ataagtaata
    22021 ttataaagca ttctaatgct acagaggcaa aaataaaatt aacattttta caaaattata
    22081 tagagctaat tgttagtgat aatggggagg gattcgatat ttctgaatta aaaaaacaaa
    22141 gaatcgatgg ggaatctggt tttggtcttt gcagcatgaa agagaggaca gaattattgg
    22201 atggaaagtt tgtcattcaa tctaaaccta agaaaggaac tcgatattat tttagaatac
    22261 cttgttaatt atttgttggc ttggaccatt gattgggttt tttgatggag taaaggaggt
    22321 aaaaatgaaa gatattgtaa gtgttatcat tgcggatgat cattcaatgg taagggaagg
    22381 tttaaagcaa ttaattgaat tggaggactg gattagggtc gttgatcaag ctgcaaatgg
    22441 tctggaagtc attgaaaaag cttataataa aaatccagac attatcttaa tggatattaa
    22501 tatgcctgtc cttaatggta ttgaggctgc aaaaaggtta aaaagaaatg cttgtcattc
    22561 aaaaattatt atgctgacca ttcataatga agttgagtat ttgtttgaaa cagtggaaat
    22621 tggagtggac ggatatatat taaaagattc tgaatcagaa gtgcttatta atgcaattaa
    22681 atctgtttat aatggaaaat cttatattca gcccaatatg gctgcacagt tggtaaaaag
    22741 ggtaaatagt atccatgggg attttaatgg ggaggatcat aataaaaagc atttaacaaa
    22801 aagagaaata gaagtactaa agctgattac tgaagggatg ttgaataagg aaattgcaca
    22861 aaaactgtgt attagcgaaa aaacagtaaa gaatcatgtt tctaatatat ttaagaaaat
    22921 aaatgtttgt gacagaaccc aggcggctgt ttttgccata aaagaaaaca tagtaaacat
    22981 taactaatac ttaattttat tctgtgaatt tatatcttct ttaccttgat tttttttgtt
    23041 actatgacta aaattatatt gaaaggaagt gattactctc gtctttttgg cgagagtttt
    23101 tagtattata gaagaaggga agaaatataa tggattttaa attagaaata gctaaactgc
    23161 tttccggaca atttacggat ttaagccttg aagaaatcct atcgatgata gaaatacctc
    23221 ctaatccgga tatgggagac tatgcttttc cttgtttccg tctggcaaag atcctgagaa
    23281 aggctccggc gcaaatcgct gaagatgctg ttaaaaatat tgaaaaagca gattttatag
    23341 atgaaattaa aaatgttacc ggttatataa atttttatat caataaatcc gtttttgctg
    23401 cagcagtgtt atcagaactt ttaaaagcta aggagtccta tggtcaatcg gatattggaa
    23461 aaggaaaaaa tatcgttatt gattattctt cacccaatat tgcaaagcct tttcatgtgg
    23521 gtcatttaag atcaactgtt attggtggaa gcatatatcg tatatttaag gcattaggct
    23581 ataataccat tggtattaat catttaggtg attggggtac tcaatttgga aaacttattg
    23641 ttgcatataa aaagtggggt agtaaagaag ccattgaaaa aggcggcatt aaagaattaa
    23701 tggatatcta tgttcgtttt cacagtgaag ccaaagaaga tccttcttta gaagatgaag
    23761 ccagggaatg gtttgcaaaa atggaagctg gtgatgaaga agctctttct ttatggaact
    23821 ggtttaagga tatcagctta aaagaattca acagggttta taaactttta gatattagtt
    23881 ttgattctta tgcaggtgaa agtttttata atgataaaat ggacgctgtc ataaaagaat
    23941 tggaagaaaa gaatctgtta aaagaaagcg aaggggcaaa aattgtagat ttggaagacg
    24001 ccaatatgcc accttgctta attactaaaa aagatggcag tagcttatat gcaaccaggg
    24061 atattacagc agcaatttac agaaaaaaga cctatgattt tgaaaagtgt atttatgtga
    24121 ctgctcttgc tcaaaatctt cactttgctc aatggtttga agttataaaa agaatgggct
    24181 atgactggca tgacaaactg gttcatgttt cttttggtat ggtgagtatg gaaacaggaa
    24241 agctttcaac cagagctggc aacgtggttt tacttgaaga ccttttgaat gaagcaattt
    24301 ctaaaacctt agatataatt aatgaaaaaa atcccgattt agaagataaa gaacaagtag
    24361 ctgaaaaagt gggtattggt gccgttatct tcaatgattt aattaacaat agaattaaag
    24421 atgtggtttt ctcatgggat aaagttttga actttgacgg ggaaacaggc ccttatgttc
    24481 aatatactca tgcaagggca tccagtgtct taagaaaagc taatgttgac atcgacagct
    24541 tagacattga ctattcttta cttaccgatg attcatccat tgaggtgatt aagcttatag
    24601 gtcagttccc tgagaaagta aaagatgctg ctgataaatt ggagccttct tttattgccc
    24661 gtcatattgt tgatttggca caggccttta ataaatttta tcatgataat cctgtgcttg
    24721 ttgaagatga aaaaattaga aatgcaagat tagcgcttgt ttattctgtt aaaacagtaa
    24781 ttaaatcagg tctttatctt ttaggaattg ctgctccgga aaaaatgtag ggaggagtaa
    24841 aatgaaaaaa attaagttat tttataatcc tgtctctggt gataagaaat ttccgcagca
    24901 actggatact tttatatata aatttcaaaa tgcaggttat gaagttgcaa tatatcgaag
    24961 tgaattcaat ggtgatataa cgaaagcagt aaaaaagatt gttcctgatg agtattccat
    25021 tttagcggtt gctggcggtg atggaaccat taatgaaatg gtcaatggga tggtggaagc
    25081 aggcattgat atacctcttg ccattttccc tgttggaact gccaatgatt ttgctaatca
    25141 tttaaactta cccaaatcgg ttgctgactg ttgtgatgtt gttttaagaa ataacattaa
    25201 agaaattgac attggcaggg ttaataataa atattttatt aatgtatgca gtggtgggct
    25261 ttttacttct gtatcccaga gtgtagactt aaatgttaaa aataccctag gtaaattggc
    25321 ttattatata aaaggtattg agcaacttcc taacttcaga ccttttaagt tacgtattga
    25381 aacttctggg gatacgatag aagacttttt ttatttgttt ttggttttaa atggcaatgg
    25441 ggcaggaggt tttgagcgtt tatcccggga tgcttccgtt caggatggtt tatttgactt
    25501 tgtaggagtt aaggcaaaac cttttcatga attggctatt ttgtttttaa aaatattaaa
    25561 aggtgagcat ttagatgatc ctaatatatt atttatccgg gataaaaagt ttaagattac
    25621 ttgtttagaa gaagacagaa gattttatga atctgatgtt gacggggaaa aaggaccaga
    25681 tttaccgctt aatatagtgg tcgttgagaa aaaacttaaa gtatttacta atttataatt
    25741 aagattctta tattcatttt ttataaattt aaaataaggt ttactatgat taaaaatcca
    25801 agatattcag gcttggattt ttattttgat ttttttatgt aatgtttcat gtgaaacatt
    25861 gcataaaaat taaaagctaa tgtttcacgt gaaacatttt tacttgtatc aagatatctt
    25921 tgtttcacgt gaaacattac taactacagt aaaaataatc taaatttttt taaaggattt
    25981 cattcaataa aaaacatgct ataatagatt aaaaagaccc atgtgaggtg aaaaatttgg
    26041 gaactacaat cgcggtattt aaccaaaaag gcggggtggg aaagacaaca acgacggtga
    26101 atgtctcagc tggattagca gaaaaaggag caaaggtcct tgctgtggat atcgaccctc
    26161 aaggcaatac caccagtggt ttaggcgttg ataaaaacaa attaaaaaag acgatttatg
    26221 atgttctttt aggcgattta tctatgaaag aggttaaaaa acctacacca atgaaaaatc
    26281 tggatttaat tccatccaat atggatctag caggagcgga aatagagctt ttagatatca
    26341 ataaaaggga atatattcta agaaaaaacc ttgaggatat aaaaaatgat tacgattata
    26401 tccttgtaga ctgtcctccg gctgtcaata tactaacctt aaatgccctg gctgcggcca
    26461 attctgtatt agtacccata cagtgcgaat attatgccct cgaaggctta acacatctga
    26521 tcagtacgat taaattagtt cagcaaagga taaacccgga attatatata gaaggtatta
    26581 tttttactat gtatgatgca agaacgaatt tatccttaca agttgtagag gaagtcaaaa
    26641 gatattttcc aaaagaaatc tacacgactt tgattccaag aaatgtaagg ttgggtgaag
    26701 cccccagcca tggactacct attatgcttt acgatgaaaa gtcaaaggga gcagaaagtt
    26761 atcgcttatt ggttgaagaa atattagaaa ggaaggaagc atagtggcaa aaagaggatt
    26821 aggaaaaggg ctttccgctt taatctcttc agagatgtct gaggaaaatc aaattaaaat
    26881 gatttccatt aattcgattg agcccaacaa agaacaaccg agaaaatatt ttgatgaaga
    26941 taaattagaa gaactagcaa gttccataaa agaacatgga attattcaac cattgattgt
    27001 aaaacaagaa gaagattatt atgtaattgt agcaggtgaa agaagatggc gggctgccag
    27061 gttggcaggt cttaaagaaa ttccagtaat tattcaggat tacacaacaa aagaattaat
    27121 ggaaatatcc ctgatagaaa atattcaaag ggaagattta aatgccatag aagaagctaa
    27181 ggcatatgaa acccttatga atcaattctc cctgactcag gaagaactgg ctggcaggat
    27241 aggcaaaagc cggccggcta tcgccaatac cctacgtctg cttcagcttg atgagaagat
    27301 acaagagctt atcatacagg gggaaattac ggaaggccat ggcagggcta ttttaaggct
    27361 tccagataaa gaagcccaat tagccttggc aaaaaaaata ataaaagacc atttaagtgt
    27421 cagggaaaca gaaaagaaag ttgatgaaat gcttaaaaag ccaaaaaaga aaaccataaa
    27481 agatgaaaag gaagtcatct acaaagaaat agaagataaa ataaaaaata tacttgggac
    27541 taaagtacag attacaagag gaaagaaaaa aggtaaaata gaaatcgaat attataatga
    27601 aaatgacctg gaaagaatct tatatttact ggaatcaata cgcgaagcta actagaggtg
    27661 aactgaatga atttaataaa aatattgtta gacaatcaag tgtggctctt aataacaaca
    27721 agcattattt cattagtctt attggtcata acgattctgc tgatgataaa aatcaactgt
    27781 ttacaaaaga aatatgatta ttttatggga ggcgaaaaag aaaagcctat agaagactta
    27841 cttttggcat atatcgacag agtcagtcag gttgaaaaag ataataaagt cattagcaga
    27901 gatataaaaa acataagaaa agatctgagt aaatgtatta ataaagtagg tatcataaga
    27961 tataatgcct ttgaaaatat gggtggtgac ttgtgttttg ccattgccct tttagatgaa
    28021 aacgatgatg gtattgttat caatggaatt cactctagag atggttcaaa tacttttgca
    28081 aaacccatta aaaaaggcac atcggcttac accttatcaa aagaggaaaa agaagcgatt
    28141 gaaattgcgc aatctaactc aaaaatgaat atttaaggag aaatagaatg gaaattatat
    28201 atattgtaaa tcctacagca ggaaatggaa aagcaaaaat gcttattcct aaaattcatg
    28261 aaatcatgaa ggataaaaat ttaagttatc aagtaataga aaccactaaa aaaggcgatg
    28321 caacgcttct ggccaaaaaa gctgttaatg aagatgccaa aaaagtgatt gctgtaggtg
    28381 gagacggaac aattaatgaa gttgtcaacg gaattgaagg cagtaaggtt gttcttggga
    28441 ttgttccggc aggttccgga aacgatgtgg caagattatt aaagcttcca aaaaaataga
    28501 agattccata gaatttgcaa tcaatggcag agtaagaacc gttgatttag ggaaagttta
    28561 taaaggctat tatatcaata ttgcaagtat cggccttgac gcagaaatag ctgcttacac
    28621 ccaggtgagt aaaaaatatt tcaaaggcaa atgggcatat attgcctcat taataaaaaa
    28681 tttattttgt tataaaccca ttcatgctgt tataacaata gacggcaaag aaatggaaag
    28741 aaatcttctt ctatttgccg taggaaatgg tcagtactat ggaggaggat ttaagattct
    28801 tcctcaggcc atcatggatg atgcctattt tgatgcttgc atcgtatccc atattcccag
    28861 atggaaaatt cctttggcat taccagcttt aatcaaagga gaacacgata agtatccatt
    28921 tatagaattt ataaaattaa aaaagataaa aataaaaacc agcaaagaag tcaaattaaa
    28981 tttagacgga gaaattagcc agggtagtta ttttgaattt gaaattatac caaatgcatt
    29041 aaatatcata ggtcattaag gtgaataaaa acacctccag gttaaagaat aatacaggag
    29101 gtgtttttat gattataggg attcaaaggg gtctggatgg actaaaaaag aaactggagg
    29161 aaaaagggta tcaagtttat tttgaagatg aataccctta tgccatagat gcttttatat
    29221 ttaataagtc cttgactctg ccggatatgg attatcatga tagcccagta cttacggctt
    29281 ctttagaaaa aataccgtgt caaagtaaca aaggcgtttt acttatttgc agtaaaaaca
    29341 aaagcctgga agaaatcgaa gaaatcttaa ataacagggt ttattctcca ttattttaaa
    29401 aaggcagctt aatttttaag ccgtcttttt ttctgttagt gaagattcat tgattacaac
    29461 agtatttcga atgtgattta aagccttcct aaatgaagga ataaaaagaa taacaagagt
    29521 aagagccatt tctataccga tataagacaa attatacagg aaagaataag cgacagcact
    29581 ataaccttcc caggcataag aaccaaagaa aatccagcct gataagaaag aacaaataaa
    29641 gcgaccagta gaaccaacaa tataacctgg gataatggat ttttgtcctt ttctgaaaaa
    29701 accagaaagc cccaaggcgc caaaggctaa aggatagtca agcagtagtt gtgctgggtg
    29761 aaccacataa ccgtctataa gaagctgtaa agttccatgg gttattccgg caatgattcc
    29821 ttcaaaaggc ccaaaccaat aaccaacaag tacaataaac aacatcgaaa aaggtgtaat
    29881 agaaccgcct tgtggcagtt ggaataatct aatataagat aaaatggtcg caagggcaat
    29941 ggcaatacct gagtaagcaa ttgcttttac agacagattc tttttcttgt gaagattaat
    30001 cacaaaaaga ccaattaaaa taattgtcac aactgcagtt atagatttac ctaacaaaga
    30061 accaaaaaat tcatttaaaa cttcttcaga aaacataaaa aaccctcctt gtacatatac
    30121 acttgtccgc aggaggtttt ccaaatgacg cttcccttcg ccagtattac ctggatcagg
    30181 tacaaagggt tagtgcaata ttgcacactc tcagccacaa gggctcccct agcggacgat
    30241 atttaattta agaatgcatt cttaaaagca taatataaca aaataataca tttgtcaaac
    30301 gtataatttg ctaattttaa tttagctaat gcggtttgtc tttttgttat atcatgctat
    30361 aataatataa tgtcaaagaa tatgtcgttt attaagattt tttgtatgtt aataatctta
    30421 ataagactta tatatattag aaaatctgat gaaaggaaag aatcaaatga actataaaat
    30481 gcttgtaacg gatatggatg atacattact aatggatgat ttaaccattt caaaagaaaa
    30541 taaagaagcc atcagcaagg ctctagaggc tggtgtcaga gtgatcctgt gctcaggaag
    30601 ggcaaccaat tctatggcaa aatacgttaa agaattagaa ttaaacaaga aagaagaata
    30661 tgccatttcc tataacggtg cagtgatttt tgatatcagg accttagaac atatttatga
    30721 agaaaatata tctcatgaag atgcaaaatt tctatttgaa attggtaaga agaataattt
    30781 atttgttcaa acgtatcaag atgattatgt atatgttgaa aaaaagaccc cttattctga
    30841 tcgctatgcc aaaataagtg gactagaggt aaaagaagtt ggtgacttgt caaaatggct
    30901 taaaggagat gttattaagg ttttatttca aggcgaaaaa gaaaaattgg atcaactggc
    30961 aaaagaatta aaacctgtcg ttgaaggaag atttcatatg ttcttttcta aacccactta
    31021 tctggaattt accaatattc atgtcaataa aggattaact gtaaaaaggc tcagagaaaa
    31081 gctaggtctt aaaaaagatg aggtcatctg tgtaggtgac agctttaatg atttatatat
    31141 gataaaagaa ggcggcctgg gagttgctgt tgccaatgcc catcctgcga tcagacaaga
    31201 agcagactac attactaaaa gcagcaacaa cgagcatgcc attaaagaag tcattgaaaa
    31261 atttatatta taatttctat caagagtaag atatattata ctaaaacacg cattcaaata
    31321 gaaaaataaa aaaagacctg ggcttcaggg gggagaagca ccaggttgag gggggattta
    31381 gcgatgtacc tctcgctggg tacactaaaa taataaccat tttaggtaat ttctatacat
    31441 taaatccccc tttttaataa aaatttatat taaaaaattg cattaataat ctggatggct
    31501 tctttactga ccaccttata ggtgatttcc aggccagatc tgacaccctc aataattccg
    31561 gctgatttta gttttgagat atgttgggag acggtagact ggggaaggcc aagacaagtt
    31621 tgcatataag aaacgttaca accatcatta tctaatagcc cttttacaat acaaagtctg
    31681 gcgggatgac caagggcctt taatatttca gctttttttt cgtacgcttt tacgtccata
    31741 agatcacctc ggagttttta atattataat attatgataa catgagctag tcaataggat
    31801 accatttata taaagtaaat ttctgccttt tattgatata acagattaat catgatataa
    31861 taactttaca tggagaagta ctcaagtggt cgaagaggtg cccctgctaa gggcataggc
    31921 tgcgaaagtg gcgcgagggt tcgaatccct ccttctccgt tttttaaccc tgtatttttg
    31981 aatacagggt tttttatttt agcatcagat ttttttcatt taaacacgtg tttatatttt
    32041 tataatataa tagttgaaat atttaaaaat tgttgctata ctatatttta aaaataaacc
    32101 ttatttaggt aaagagagaa atttttatgg aggggtaaaa aaatgcaaga cttgttgatg
    32161 gattatttaa cgcaaattat tagtaatatt tcacaagccc aaagtgaagt gttaaaagaa
    32221 aaacaattga aatataaaat ttctccaaac gaattaagga ttatagaaat cgtaggaaca
    32281 agcagaaaac caaaaatgat gaaagacatt gcagaattga tgtcaatgac taaaggaggc
    32341 atgacatttc ttattgacaa gctggagaaa aaaggcgttg tcagaaggag acaaaacgat
    32401 tttgacagaa gggtattata tattgagtta acagaagaag gagaaaaaat cttcgaagat
    32461 tataacagaa ataagtacgc aatcttatat aaatgggttg aaaatatgaa tgcttcaacc
    32521 aaaaaaatta tgaccgaaga attccacagg gctttacaac tggctgaaaa gtctgtttag
    32581 aaaaaatagt actttttgat gtatagaatt tatcacctcc atccaaacta gctttgaatg
    32641 gaggtgtttt taatggacga taaaaaatta agattaaaaa aagacattga cttaagccgt
    32701 aattttaagg atgaattaag tgcagagctt actgataaaa caacggaaga taataaaaaa
    32761 gatcaatctg aaatggcctt taaaataatt gaaaactgca atccggatga ttttgagtaa
    32821 ggaaaaaagt aaagtcatta tgactttact tttttattgg aattaaataa ctgttaattt
    32881 aatttgacag tttacatatt tgaatataca attaaaaaca gaccagagta taacagtgaa
    32941 attacagaaa ggatggaaag aatgggctca ctagtggaca gcttttcttt gcgtgagatt
    33001 aattttgcta aaacaagatt aaacatttta aaggtggtaa aagacaggat cactcaaaaa
    33061 gattttgatg atattacagt cgatgaaatt tgtaaatatg ccgaaatatc cagaggcact
    33121 ttttttaatt attttcccag taaaaatcac atttttaatt attatataag ggtatggatg
    33181 atacaattag gccttgaaat ggaaaagcaa aagtttaaaa gttcacagga aaaaattaaa
    33241 aagatttatg ataaagtgat agaagaagac acaaattatc ctggttttat tgaaaattac
    33301 ttaaaatact tattagaatc aaaagaaatc aacaatgaga taaaattaac ccaggcagaa
    33361 cttgcctata aattttcttt agaaaacatt aaaaaagaaa atatggaaat gtttaacagc
    33421 ttgtcgatag aaaaaatatt tgaaaacttt ataaacgaag gcgtaagtaa tggagaattt
    33481 aaaaaagata ttaataaaga aaatacttta ttattgctgc tctcattatt ctttagtccg
    33541 gctgtcatga aatttatcaa taaatccttt gatttaaaga gatattacga agaagctctc
    33601 gacaacatat tccataatgt gtaataaaaa tagcattgta ttttataaat ttttatgtta
    33661 caattgttta cgcccaaaaa gaatgcacgc atagctcagc tggatagagc atctgacttc
    33721 ggatcagagt gtcgagggtt cgaatccttc tgcgtgcgtt aacccctatg tttatagggg
    33781 gtttttttct atggggagag gaggatgcgt atggagacct atatgagtgc agtaggtttt
    33841 ggaagtataa agaaaaagaa acaatgggac aagttaatcg aagaagtgtt aaatcatgcg
    33901 gataaacgtt atgtaaccaa tcatgatgaa gatcatgttt tcatagaata ctataaaagc
    33961 tatggaaaaa atattggggt cgttttaaga ggtctattag ataatgaaaa ggatatcgat
    34021 tttgaaacct gcgatcctta ttttgaatca aaatacatca tggaagtttc agaagtagaa
    34081 gtcgaagaag atgaagagga ttatttttat tttgctgcta cggaagaaga agaaagtggt
    34141 gtagaaatcg tttttcaact ccaaaatatt atagagtatc tggaaattaa agatgacaaa
    34201 catgccagta ttgaagggat taaagtagta ggtctggcat caaaaggaac cattattctt
    34261 cccgtagaaa aatcagaaaa tgatttagag tatgaaaatg agcaaaaaga atggagaaaa
    34321 gaacttttaa aaagaatcaa ggccggagat gaagaagcaa gaaaaatttt agaaacagaa
    34381 gcagaagaag cggctgaaat tatagaagaa agacttcggc atgaagatat tttatccgta
    34441 atagaaggct attttattcc ggttgattat ctggaagcaa cctattctat cttaggtgag
    34501 ataaaagaga tagagcttat tattaatgaa gaaaccaggg aagaaattta ttggatgctg
    34561 gtagaaagca tgggcatgtc catcgaaatt gctataaatg ccaatgaatt agtgggtact
    34621 cccctggtag gtatgagatt tatgggcata tgttggatgc agggaacaat tgtattttcc
    34681 taaagtctta ataatccttg actatccgct tataagtctg taaaatatta aagtggctat
    34741 taattattga ccaaatttgg gtcaatttta atatttgttt ccgtagctca gtaggataga
    34801 gcgactgcct cctaagcagt aggccgtggg ttcgaatccc gccgggaaca aagataaaac
    34861 ctcgtatatt taggtatacg gggtttttta ttcattaaaa acttttcata tcaagtaggt
    34921 atctattagt gacaaaggca aggaataact ttataattga ggtatacata atctaaaaag
    34981 ggtggttgga tgaaaaggtt taaacatttt ttactgggtt ttgtggcagg agcactcgtt
    35041 tttacaagcc cgcaagttaa ggcttttgac ggtttacaaa ccataaaagt attatttagt
    35101 aatataccca tttacataga tggaaacaga gtagagcttt cccataaagc catattgcat
    35161 gaaggaacca cttatttacc catgcgggat attgcagagg ccttgggaaa agaagttctc
    35221 tacaataaca atacaaaaga aatttatatt tatgaaaaag aaggtaaaaa cagtgataag
    35281 tcaaatacaa agacagataa taatgatcta tcccgaaatc agcaaatttc cggggaattt
    35341 agcgtaaatg gcatagccct tggagacagc gctgaagacg tatctgatgt attgggtgag
    35401 cccaacaggc aggatgtttc tgaatatggt tttacctggt atatatacaa taaggactat
    35461 agcaactata tgcaagttgg tataaaagat aacaaagttg ttgcccttta tacgaattca
    35521 gtatccaatt ggaactttaa aaaaggaatt cgttttggtt caacggccgc ttctgtaaga
    35581 aaggcttatg gacaacccct tagttttatt caaaaaggac aatacagatt ttattttaat
    35641 tataaaaaag gcgaagccga aacctattcc attgatgatg catttgttac attcttttat
    35701 gatattcata ataatcaaac agtaacatcc atactaataa tcgataaaaa tacagagctc
    35761 tcccatgaaa gtctttatgg agaagctagt gaagaactta gaacagccta tacttatcaa
    35821 atttttgacc tgaccaatgc tgtacgggta agaaatggct taaaagctct gacatgggat
    35881 gaacaggccg ctaaaagcag ttaccttcat tcaaaagata tggcagaaaa caactttttt
    35941 gaccacgata atttgaaagg agaagatccc tttgacagaa tgaaaaagca aggaatacat
    36001 tttatgatag cgggtgaaaa tatagcagga gggcagacta atgccattta tgcccacgaa
    36061 ggctggatga attcaaaagg gcacagagag aatatcttat ttgaaaactt tgaaaggctt
    36121 ggagtaggtg tatactttgg aggaagatat aaaatctatt atacacaaaa tttttatact
    36181 ccattaaaaa gataaatttt aaaaataagg agtttaccca tggaggataa agcaattact
    36241 tttatgaaag aagctttaaa actggccgaa aaggccaaag aagcagatga agttccgatt
    36301 ggtgctgtta tcgtttacaa tgacgaaatt ataggaagag gctacaatca aagaaattcc
    36361 caaaaaaatc ccctggccca tgcagaaatc caggctatag aagaagcggc aaaatatata
    36421 ggggactggc gcctggaagg ttgcagcatc tatgtgactt tagagccttg tcccatgtgt
    36481 gcaggtgcca ttgtgcaggc cagaatcgat aaaattatct ttggtacaag aaatcccaaa
    36541 gcaggctgcg gcggttctat tttgaatatc ttaaatgagc ccaggtttaa tcaccaggtc
    36601 gaaattatcg aagggatttg ccaggaagaa tgtagccaaa ttcttaaaac tttctttacc
    36661 caaatgagga ataaaaggtt aaaaatggag gttgacatat aatcagaaag attgtatact
    36721 aagaaatgcc accatttacc cccaaatgcc accatttgta ggtaaatgga gcacttacaa
    36781 gtatttacta gcattaatat gtcataaagt ttccgtgcta gccggggagt tagcggtgcc
    36841 ctgtacctgc aatccgctac agcaggggcg atgcctgccc gaggcagtat tttttagggt
    36901 ctggcttttg caagtggcga tgagaatcgg gtcttgcgca acagaagttt gtgaaccgtg
    36961 tcaggtccgg aaggaagcag cactaagcaa atatctctgt gtgccgcgag gtagcctaat
    37021 ttgagctaac tgcaaaagta acgcctgggg agcgctgtcg acggcaggtg cacggattta
    37081 ttatattcat acaacagatg gaaaaccgtc tgttttttta atgcctaata gcctttttga
    37141 ggctgaattt ttttatactg ccttttggac ttttattata tataaagtta tatttgtggt
    37201 acaataataa aagtagtggc gcttttagtt ttttgataaa aggggtggat ttatgtccta
    37261 tatggcttta tataggaaaa aacgtccaaa gactttcgaa gacgttatag gacaagaaca
    37321 tattgtgata actttaaaaa accaaataaa ggccaatcgc attgcccatg catatttgtt
    37381 ctgcggaacc aggggaacag gaaaaacttc aacggcaaaa atatttgcaa aagccgtaaa
    37441 ctgtgagaat tcccaagacg gggaaccctg tggccactgc agtttgtgtc agtctataga
    37501 cgaaggaaga acgatgaata tcattgaaat agatgcggct tccaacaatg gggttgataa
    37561 tgtcagagaa ataagggaag aagtaaaata tacccctaca gaaggaaaat acaaggttta
    37621 tattatcgat gaagttcata tgttatcgac gggcgctttt aatgctttac tcaagactct
    37681 ggaagaaccc cctgcccatg ttatttttat attagccaca accgaccctc aaaagattcc
    37741 cgccacaata ctgtccaggt gccagcgctt tgactttaga agaatatcca ctgacagtat
    37801 cacaaagcgg cttaaagcct atatgaaaga agaaaatata gaagtcgaag acaaagccct
    37861 ttcatatatt gcccgcttag cggaaggttc tatgcgggat gctttaagta ttttagacca
    37921 gtgtatttcc tttaattacg gagaacgtat cagttttgaa aaagtcctgg atgttatggg
    37981 tgcagttgat tataaggtat tctttgaatt tgcttccatc ttaaacaaga aagaaagctt
    38041 agagaccatg gaacttatcg acaaaatcgt aatgcaggga agagacctga gtcagtttgt
    38101 catggacttt attgcacacc ttagaaatct tttaattgtc tccagcagtc aggaagcaga
    38161 aaaaatctta gatttaagtc aggaacaaat tgagctttta aaagaccagt ctaaagaaat
    38221 agcttcttca accattttaa gatggataag aatcttttca gagttatcca atgaattaaa
    38281 gtatacggac caaaaaagag ttatgcttga ggtagcagtt atcaagatct gccagcctca
    38341 aatggatgac tcaaaagatg ctctgcttga acggataaaa gtacttgaag aaaaactggc
    38401 agaaggcata gtcgttcagg caacagaaaa gccaaaaaca caggaaaata aaaaaagcag
    38461 tccaaaagca aagcccaaaa aacccaaagc agttcctgag gatatcaaaa aggctgtaag
    38521 cttgtggaat gacattaaaa caagtttcag tgactctcta aatggtcttc ttatggatac
    38581 aaaagcggct tatttagaag atgatgtgta ttacatcgtt tttaaagatg cctttataat
    38641 agaagcaatt aaaaaatata aagatgaaat aattgccagg attgaagagc ttctaaaaaa
    38701 agaagtgaat gtccatttta tttcagcaga agcttatcag gaaaaatatc aagaaatcta
    38761 tggcgattcc cctgagaaat ccgaagaaag ggaccaaata gaagaagtca ttgaatactt
    38821 tgaaaaatta ggtgtttcat acgaaattaa gtaagatatt tattttttgc tttaatcatg
    38881 ctattatatt tgttataaag gagtaagagg aggataatta tggcaaaaag aaatttcggc
    38941 ggaattccag gaaatatgaa tagtctttta aaacaagcac aaaagatgca aaaagaaatg
    39001 gaaaaaatgc aacaagaact tgaagaaaaa gatgttacgg cgacagctgg cggaggagcg
    39061 gttgaagtcg tatttactgg taagaaagag ttaaaatcta ttaccataaa accagaagtt
    39121 gtggatcctg atgatgtaga aatgcttcag gatttaatcc ttgcagcagt taacgaaggt
    39181 cttcgccagg tggacgaatt agccaaccag caaatgagca agcttacagg aggattaaat
    39241 ttaccgggag gactctttta gatgaaccat tatggagaca caatgtcaag attgattgct
    39301 gccctttcag atcttccggg ggttggcgta aagacagcac aaagattggc atttcatatc
    39361 attaacatgc cggaagaaaa tgtagaaaat ctttcgaaag ccattataga agccaagagg
    39421 aacgtaaaat actgcagtgt atgctgtacc ctgacggata aagaaaaatg tgacatctgt
    39481 tccgatgtaa gaagagacag caaaaccata atggtcgtcg aagacccaag agacctggca
    39541 gcttacgaaa aaaccaaaga atataaagga ctttatcatg tcctgcatgg agccatatct
    39601 cccatgctag gtgttggtcc taaagatata aaaataaaag agttgttaga aagattaaaa
    39661 agtgatgacg tcgaagaagt catcctggca accaatccaa atatagaagg cgaagcaacc
    39721 gccatctaca tcagcaaact cataaagcct ttaaacataa aagtaaccag aatcgctaat
    39781 ggcgttcctg taggcggaga cttggaatac atagacgaag tcaccttgtc cagggctctg
    39841 gaagggcgaa gagagatgta gattttttaa atggctggaa aaaataaaat catttcatca
    39901 ttataaaatg aaaataaaaa gcaattggaa acatggttta gcagaattct ttgagactct
    39961 gtgaaccatg tttttattaa gactttttaa aatttgcagg ttcacttaaa aggatgggct
    40021 atattgattt cacgtttttg atgaaacaga aaaatagcaa aacccttaat ttatttcatg
    40081 aataaaggat gatggcatgg attatcatca tttaatgaat cttaactgtt atatagagtt
    40141 ttgtggatta acagaacctg aaacaaattg tttaagattg tattttgcac gttgcaaaac
    40201 agaagagaca gcggaagatt taattattgg agatacagtg attaaaaaca cttatccaat
    40261 tattatagat aaggatttac cagttttgca actggacttt gaatcttata tcgcttattc
    40321 tgtaaccaat gagtcattta cctcatggga tgagtatgag caatttgaag gaaaggcatt
    40381 taggatttat acaaaatcaa ggtatttgga ttttattaag gaacacactt ttgcagctca
    40441 agtttgtgta gatgtgtatc cgccttttag acattatggg atagtatgct taaatcatat
    40501 tattaatatt gtttcaaccg atacaccaaa aattacagaa atatataaac agcaataaat
    40561 atcaggaagt acgagagctg ttttttcaga tttataaagt taaatattag tcctgagcct
    40621 tttgatgttt tagcaggagg aaaaattcaa tgaatgatat acgtaaaaaa gttatcgatt
    40681 ttttggaaaa gatgaatatt ccttatgata tgattgagca cccggcagtc tatactatag
    40741 atgaaatgga ccagttaaat attaacatta gtaataaaga aaataatgaa attgttaaaa
    40801 acttatttgt ccgggatgat aaaaagaaaa attattactt aattgttctt cagaaagata
    40861 agcaggcaaa ttttaaagaa atcagaaaag cactggattc cagaccctta acctttgcct
    40921 cagaagaaga tttgcaaaaa tatatgggac tgacaaaagg ttccgtatca ccttttggaa
    40981 tattaaatga tgcagactgt aaggtagaga ttgtttttga taaagatatt ttatcttttg
    41041 agcgaatcgg agttcatccc aatgataata cagcaacggt ttggcttagt ctgaaggatt
    41101 tagagaatat tataaaaagt catggaaaca gcatcagcta cataaatatt taatatttaa
    41161 agaataggtt attaaagctt atgaaatatc atctttgtaa aaaacatgct ttaacaggga
    41221 aaagcttcga tcggcaaatg ctaataaata ttttgaatat agtttaatct taataaattt
    41281 aacagttttt ttaatatggt ttaaagtttt ttaaaataaa aaacatagat acatttaatt
    41341 taaacaaaat tatgtgtaag ataacattat atcataagca tttgtaaatt tgtatagaga
    41401 aaggaagatg ttatggaaat caatatgaag caacagaaga ggagtttatt aaattttaca
    41461 tttattttca caaaaaatat aataaaatgg tgcgttttaa ggtaaagtat ttgttatctt
    41521 tttttatgca gcaggaatat ttttaatact ttcaaacatc agaaagaata cctatacttt
    41581 tatattgggt gttagtgttg caattttgtt ctttatatta tggataatag cttttcaaaa
    41641 aatagaagaa aaaaataatc agaaagaaag cggcctctct tttaaaaaaa ggagacagga
    41701 ctcgcctgct aggtaagaaa attatgctta tcggtgaaaa tggtattaag gttattggtg
    41761 aaaactttac tgaaatgata ccaaaagaaa tcatatcaga cataggggta tatgatgata
    41821 tgatacttat tttcttaata ggttataaga gttattttat tccaacaaga tatttaacaa
    41881 gagaaaataa ggagcaattt ataaacaaat taatggcttt aaaatagaat aattaataca
    41941 tatatctaaa tttagtatcg gatacgaata tggtattgat attaagtcag gagatagtgc
    42001 atggtctggc ggaatcaatc cgggagtaca tatcaggaac aatcttgtta tagttttgga
    42061 agtgttgtta tcataaatat acctgataat gtcctggcag caggggattt atgcaggtta
    42121 tccgtgtgat tatagaacag gataagttaa attctattgt ttattggatt aggaatagtt
    42181 gacatatttt taattttttt atataataat tgctacccaa tcaataaagt aataaaaaga
    42241 gggtgatccc taccctgggt gggatttaaa agtggaagag gggtttaaaa agcggaggac
    42301 cttactataa gtaggagcaa aaaaagcggt gagtccctac cataagtggg aattaaaaaa
    42361 agaggagttg ggcaaattgt tcagctccta tattattgca gaaaggtgat gagagtatgc
    42421 atttatatct ctatcatgta gatgcctcct actgtgattt tctccgcaaa tttgaccaga
    42481 aagttcttcg tactcttgaa caaaaaaacg gccttatgta ggtcttgctt ttgaactggt
    42541 aaatggaata aaatattaca tcccacttac ttctccaaag aaaaaacatt cagaaatgaa
    42601 aaataacgta gattttcata aaataaatag cggtatgtgg ggagccatta atttcaataa
    42661 tatgatacca gttcatgaac agagtttaag aagagtaaat aacaaaatta atctgaatga
    42721 cagcaaagaa gatatacaat ataaaaaatt attatccaat caactggatt ggtgtaatgc
    42781 aaaccgtgac ttgatcataa ggaaagccaa aaaactttat aatatgatca ctagagagat
    42841 agcacctgaa aaattagtaa gaagatgctg tgattttaaa aaattagagc ggggattaag
    42901 agaatactgt aaggcacata atctgaaatt aaataacagg tatcaaaaaa tcataattga
    42961 atatattgaa gatgatcaag gacgaacagt ggcaaaacaa aaacatggaa agatgattgc
    43021 ttgcaggtgg cttattttgc cttaggagga ttaactaaca aaataactta ttataaaata
    43081 aagaataaga gaaggtctga ttagactttc tctttttttg gatctttaac aaatagtgtt
    43141 ggtgaataaa acaagagtta atagaagaaa aatgtaatcg attggcttgg aagtaacatt
    43201 tttctaagct atttttatat ttactaaatt ttgtataata agaattctgc ttttaaagtg
    43261 aaagaagcaa ggaaaattgc gttttaaaat attaatctta ttaaaagatt ctcaaaagtg
    43321 ttgtttatgt aattatgctt aaaaaagttt ttaatataac ttacatattt ttttatatta
    43381 ttaatagaga atatcatata attagaggtg tttattaata ttgttatcta acactttttt
    43441 aaagatatta taattcttgt atttaaaaga aaaattcata tattgatata gttggtaata
    43501 ggctctaatt tggagttcaa ttaaaaggat aattaaacat caaattcacg tattgttgtt
    43561 ttgaagcgac tgagatatct aatagttgat atgacgacta cattaatatc ttggaatggt
    43621 agttttcaat tcatgaaaaa tattttctca tcaaagacat gcctaaaggt tagctagtct
    43681 gtcttattta gtgttgtaat taatttttta gtgaacaaaa tatacaaaag ccgccagcta
    43741 ttgtaaaata atataataat attgtaaaat acatttatag gagtcaaaac tgggaggtgt
    43801 aatatttttc cagactaaaa agtattttct catggatttt gcttttgacc tttatagtga
    43861 ctttgatacc acaaaatata tatgcagcca atagcattga atctgttctt actgaagaaa
    43921 cagacctgga aaaagactcc gatgcagatc acttgacggg tgattttgaa ataaaatttg
    43981 gaaggaatcc ttataaaata gatgtatata tagatgtata taggaacttt attatagatc
    44041 ttttcgaatt tgctgagcaa gtttattcta gtaaggtaga tactgacggg gacggattac
    44101 ccgatggata tgaattatta tatttaggaa caaatcccaa cagtaaagat tcagataacg
    44161 atggtatttt agacagagat gaggatttgg atgaagatgg tcttttggat ggcgatgaag
    44221 taaaacttgg tcttgatccg ttaaaggcct atacatatga gagcattttg gatagtgaat
    44281 ataaaataaa acagcattta tcagaagaaa tatttaacaa tattaaaata ttgtttcttc
    44341 agaagaggtg agctcagaag aaaaagaaga tgttaaagaa gagggtgaat atgaaaataa
    44401 aattatagaa gatgcaaacg ataaatataa cattgaaaca taggacgaaa taggttccaa
    44461 atatggttag tatcttttta agtatggtta aactaaaagc attatttttg gttgttgaat
    44521 gaaagatatg ggttatttat aagaaatttg tataaaagaa aaaaggcagc aagagaagtt
    44581 tttcttgctg ccttaatgtt taattcaggc tgtttttatt ttagacttag ctttcaaatc
    44641 ttgtattttt ataaatggta aaaccaagaa ttttaatttc gattcctgta agaattttat
    44701 gatccttttc atttgtatca tcaggataga ggctgtgata attggttacg ctatataaaa
    44761 ttgcctggta gacaatcgtt ccaccgtctt taacgtatat tgggacaggg atcagtaaaa
    44821 tcaaaataat aaggaagacg ataatcttaa tcaggatttt tttcatatgg ctcatccttt
    44881 catgggatag aaaatttaat ttatgctctt tcattttgat attttaaaaa atgggttaat
    44941 ctttttcaaa ttttacatta ttgtaaacgg taaagccaag aatttttact tctatacctt
    45001 taagatatct gatttggctt tcatcatttt catcggggta catactgtga taattggtta
    45061 cacgatataa gatggcatca taaataaccg ttcccccatc attaagatat gacttttgtg
    45121 gaattaataa tattaaggca ataaggatgg ctataatagt aatccatttt ttcttcattt
    45181 gtctcatcct ttcgtactat taaaaacttt cagatataag acgtttctga atttgctttt
    45241 gttcccaaaa agtattttga tttcagaaat agatcagtta cgattattat aagaaaaata
    45301 aaagcccctt tttggggctt ttatttttgg ctatgaaagg ataaaaaaag gaagtaagag
    45361 aggggttcct tacttccttt tttattacaa taatgcgata ccgttgcttg cgccgatgcg
    45421 gtctgcacca gcttctaata attcttttgc gaattctgca gtacggatac cgccgctggc
    45481 tttcacttct aattcatctc caacaacttg tttcattaac ttaacatctt ctacggttgc
    45541 accgccagtt gagaaaccgg ttgaagtttt tacaaaagca gcacctgctt ctttggagag
    45601 ttcacatact tttacttttt cttcgtcggt taaaagacag gtttcaataa tgactttcac
    45661 gatagcaggt ttggaagctt ctacaactgc tttgatatct tcaagtacca aatcccagtt
    45721 tttatcttta actgcgccaa tattgataac catgtccact tcttctgctc cggcttttac
    45781 acattcagca gcttcgaatg ctttagcaga agtcagcatt gcaccgagag ggaaaccaac
    45841 aacacagcag gtttttacgc cactgccctt taattcctgg gcaactaaag gtacatgaca
    45901 actgttgaca catacggaag caaaaccgta ttctttagct tcctggcaat attttttaac
    45961 ggtttcaacc gttgcgtctg cttttaaaac agtgtggtca atttttttcg caatattcat
    46021 tattgtattc tcctttatta ataaatttat tgtttttttc atcatatcat aagtcattta
    46081 aaatttcaat tgaaaccgat tacattttta aaaaatatca tgaaaaccat gaaatagtgg
    46141 ggaaaagcaa caacagcatt gacattttaa cataaaaaac cgacaaaaat caacaaaaaa
    46201 atgattgaca cttctatatt attgtgctat atttataaca taatatgaaa gcggttacat
    46261 ttgggggtta tttagcgatg aatatttacg atattgctag ggaggcaggg gtttctcccg
    46321 ctacagtatc cagagtaatc aatggaaaca aaaatgtaag ggaagaaact agaaaaaaag
    46381 tattaaaagt tattgaaaat aataattatg cacctagttc agtggccaga aatctaagta
    46441 tcggtgaatc tcagaacatt gcttttatcg ttccggacgt agaaaaccca ttttttagtc
    46501 aaatattaca aggcattacg gataaggcct cagattcaaa ttataacgta tttatgtttg
    46561 gtacgaaaga agatccagaa agagaacacc gtattttaga gtctttaaaa ggcgaactga
    46621 ttaaagggct tattattata cccgtttcag aaatagatga agaaaccgct ttcagactga
    46681 ctaatttcga aagagaaggt atcccggtag tccttatcga ccgtgatatc aaaggctcaa
    46741 attttgatgg ggtcttttct gaagatattc agggagcata tgaagcagtt gagtgtttaa
    46801 tccaggaagg ccataaaaag attgcaatga ttacagggcc tgagacatca aggccggggc
    46861 atgaccgatt aagaggttat aagatggctt tgaccaatta taatatagaa atcaatgaag
    46921 actatattat aaatgggaac tttattgaag aagatgccta taaggccatg aagactttaa
    46981 tggaattaaa agagccgcct actgcagtct tttcttcaaa caatatgacg accctgggtt
    47041 gcctgagata tctgaaagaa catggtttaa aactggtcga ggatatatct ttaattggat
    47101 ttgatgacat aaaagaacta caatatacag atattaatct tacagttgtt gcaagacccg
    47161 tctatgaaat gggtcaggaa gtgatggaac tcctcgaacg cagatttagt tataagaaag
    47221 aaggtgatag gaagaagatg atcgtacgaa ggaattatgt taagacctgg ttaataaagc
    47281 gggggtctga aaaatataag aaattccagg agggtaatgt taatgaataa agttgcagta
    47341 tttggtagtt ttgtggttga tttaatggca agaagccctc atcttccagt tcccggagaa
    47401 accgtaaagg gttccatgtt taaaatgggt ccgggaggaa aaggctttaa tcaatgcgtt
    47461 gcggcacaca aagcgggtgc agatgttgtg atgattacta aactgggccg cgacaatttt
    47521 gcgaatgttg cactggatac aatggatgag ctgaatatgc caaaagacca cattcttttt
    47581 tctgatatga ctgaaaccgg tatcgcatta attttagttg atgaaaacac cagtcaaaat
    47641 gaaatcatta ttgttcctgg tgcatgcaat accatcacag aagaagaagt aaaaggcata
    47701 gaatccgtta tcaaagaatc tgaatatgtt ttacttcaat tagaagtgaa tcagaatgcc
    47761 aatgaaatgg ttgtggatat ggcaaaccaa catggatgta aagttattgt aaatacggct
    47821 ccttatgtgc cgatttctga tagcttttta tcaaaagtat atatggtaac tcccaatgaa
    47881 gtcgaagcag aatctatgac tggtattgcg gtaaaagatc ttgaatctgg aaagcaagca
    47941 gctaagtttt tccatgataa aggcatagaa atcgttgtca tcacattggg atccagggga
    48001 gtatttgttt cttatgacgg taaagcagaa atcatcccgg catttaaagt aaacgcagtt
    48061 gatacaacag gtgcagggga tgcatttaat ggaggactgc ttgctgcttt agcagaaggt
    48121 aaagatatct gggaagcagt aagatttgca aacgccctgg ccgcaatatc tgtacaacgc
    48181 cttggaacaa caccttccat gcctgaacgc tgggaaatag atgctttctt agaggctcac
    48241 aattaaaagg aggtaataaa atgctaaaag gtattcctac tcaaattagc tcagatctgt
    48301 taaaggcgtt ggctgatatg ggtcatggtg atttaattat tatcgcagat gatttttatc
    48361 caccaaaaac caaaacacct aatggtataa gtattcaggc taaaggcaat acagcgcctg
    48421 aaatgatcga tgcaatttta caattgtttc cacttgatgt agactactgt gcttatcctg
    48481 tagaatatat ggtacccgat aaagatgctg gcatcagtat cgaacgtcca aaagtatggg
    48541 acgatgttat cgaagtggtt aaaaagcatg gatatgatgc aaaaattgtt ggtgaaattg
    48601 aaagaaccaa gttttatgaa aaagcggcaa aagcttatgt gaccgtttgc accagcgaac
    48661 gtcaacctta tggctgcttt atcttacaaa aaggtgttat gtaatttaat ataacattta
    48721 atataatttt aaaaataatg ggggattata attatgaaaa tgaaaaaaat attagctagt
    48781 ttattaatgg gcgttatgac tatttcttta gctgcttgtg gtggcacaaa aacctctaca
    48841 gacagctcaa acaattcatc tgctactgct tcatcagatg ataaaacctt tgaaattatt
    48901 tatctgactc cttctactgc atctcaattc tggacacacg ttggaattgg tattgaaaat
    48961 gctatgaagg acctggaaga aaaagaaggt attaaggtta actacacaat cgtaggacct
    49021 gctgaagaaa gccaaacaga agaatatgtt acagctttcg aaaaagcaat tgctaaaaat
    49081 ccggatgcaa tcgtaacagc aacgcttgca attgatgcaa cagttccaaa agcaaaagaa
    49141 gcaactgaaa aaggtattgt tcttaacttt gttaactgtg gtttaggtgt aggcgatgac
    49201 ggagcatatc aagattacta caatcaattc tattactgct caaacgacac cattggtgaa
    49261 atggcagctc aagctttctt agaagcaatg gaaaagaaag gtattgaaaa aggtgtagtt
    49321 ggtattaaca tgaacgttga aaatgaagct ttaaatcaca gaattcaaag cttccgtgac
    49381 tacatggcag caaatgcacc acaattaaca cttacagata cttactataa tggtaacgta
    49441 gtagaaacag cacaagcaaa tgcagaaaac attatctcaa cttatggttc tgaattaatt
    49501 ggtatgtact caggtaacaa tatcacaggt gacggtgtat gtcttgcagt tgaatctgca
    49561 ggtattggca gcaaaattgt atctgttgct gtagactctg atgacattga aatcaatgct
    49621 ttaaagaatg gaaatcttga cgctattatc gttcaggatg cttatgcaca aggttacaaa
    49681 gtaatggaaa atgctatttt aacattgctt aatggttcca atcctgaatc tagcaaacaa
    49741 gtaaattgtc ctccagttat cgtaacaaaa gataacatgg acagtgaaga aatgttgaat
    49801 ttacttgatc ctacacgttt aaaaaaataa tagaaaacat aactgaactg actggaatat
    49861 aaaatatcta aaaaaacagg aaggacagtt gtgtatgtaa aagtatgcaa ctgtccttta
    49921 aatatggagt ggggttaatg atgcaagaaa caatcttaaa aattgaaaac atttctaaat
    49981 tttatcccgg cgttaaagct ttaaataaag tgagctttga attaaaaaaa ggtgaagtcc
    50041 gggcattagt aggagaaaat ggagcaggaa aatctacctt aatcaaatgt atcatgggtg
    50101 ttgaaaaacc agatgaaggt agaattttaa tgaactacaa tggtgaatgg atttgtaata
    50161 caaatgcttt agaagcacaa agacatggtg tttttgcaaa ctatcagcat gttaatattg
    50221 cacccgattt aagtatagca gaaaattact tcttaggtca acaacctaaa acacaactgg
    50281 gatttgttga ctggaagaag atgtatgatg acagcaaaaa aattattgat aaattccaaa
    50341 tgaaagtcaa tccaacagac aaaataagaa atcttccgat tgctatgcaa gcaatggtaa
    50401 ccattagcaa aatatcagtt aatgaagata tacggattgt tattttcgat gaacctacag
    50461 cacttcttga aaatgaaaag gttgaaatct tatttaagtt tattgaagaa ttaaaacaaa
    50521 gaggcgtcag cattatttat gtatcccatc gtttagaaga aattatggag atttgtgaca
    50581 gtgtaaccat cttaaaagac ggaacttatg tagatacaaa gccaatagaa gaagtaacga
    50641 aagattcttt aataagtatg atggttggac gtgaaatgac agatatttat aatatcaagc
    50701 atgtagaacc tggtgaggaa ttattaagaa tcgaaaactt cagtgatagt cgtcatttta
    50761 aagatataaa ctttactttg cataaaggag aaattcttgg ctttgcaggc ctggtaggcg
    50821 ctggtcgcag tgaaattatg cgtgccatct gtggagttga aaagcgcctg actggagatg
    50881 tctatgtccg tggcaaaaaa gttaatatta aagacccaag cgatgcgatg aaacatggta
    50941 ttagttttct gacagaagac agacgggaag acggacttgc cctgatactt ccgattaaaa
    51001 tcaatgtcaa catgaattcc tatgatatga ttagtaaacg cggagtcatt tcactgaaga
    51061 aggaaactga aagagcagaa gactactcta agagagttaa tgtaaaaaca ccaagtatta
    51121 tgcaactggc aggtaactta tctggaggaa atcagcaaaa agttgttatt gctaaacttt
    51181 tatgcagaga tcctgacatt ttgatatttg atgagcctac cgttggtatc gacgttggtg
    51241 caaaggaaga aatctataaa atcattgaaa aactaatagc ccagggtaaa ggcgtaattc
    51301 tcatatcatc ttatttacca gaagttatgg gcttagccga ccgcttaatt gttatgtcac
    51361 aaggtaaaat tagggcagaa tatggaaaag aagaactaaa aaatctaaaa gaagaagatg
    51421 ttttacgtaa agcttcgatt gaggattagg ggaggataag taatgagcaa cacaaaaagt
    51481 gctaaaaact catataagaa tctatttcaa caaacagaat ttactttggg tgttatcatt
    51541 gtcttgctgt ttgtaatagc ttctgttttt acagataatt tcttttcaat gtacaatatc
    51601 acaaacttaa tgaagcaatg tgccataacc ggggttttgg ctgttgcaca aacctttatc
    51661 atcatcactg gtggtattga tatcagtggc ggtgcgatta caggtttatc ctgtatggta
    51721 ctcgcattac ttcaaagaga tacaggaatg aatttctggc tgacacttgt tattgcaatc
    51781 attgtatcca tcgctgcagg ttttattaat ggtattattg ttcatgattt taaggtacct
    51841 cctatgatag caactttagg tatgtcaaca attatccgtg gtatcattaa aatcatcagt
    51901 aatgcattaa ccgttagcgg actggatgaa agaatactca atatgggaaa tagcagtatc
    51961 tttggtatta ttcctacgct ggcgattata tggctgttgg tggctttagc aattttcctg
    52021 gttttaaaat atacaatctt tggacgtaac ttatacgtta ttggtagtgg tatcaacgta
    52081 gcaaaactca gtggtattaa agttagaaaa atgttcttaa gcgtatatgc tttagcaggt
    52141 cttctttacg gtattgcagg tattatgctt gcagcccgtg tacaaagtgc tcttccaacc
    52201 ggtggtgaag gttacgatat gagtgctatt gcagcagctg ttatcggtgg tgcttccctt
    52261 gctggtggac gtggagctat cgcaggaacc ctgcttggaa caatccttat ggtattaatc
    52321 aataacgccg gtgttcaatt tggattaaac acccacgttc ttgaaatcac cagtggtgta
    52381 ttaattgtat ttgcagtaac aatggatatg cttaaaaaca gaaaaaaagc ttaaatcgtt
    52441 aggagtaagg aagcacggga ttgcctgctt ccttttctta ttgagaaact gaaacaagga
    52501 accgtcccca tgtttcaccc gcgtttaaaa ttatcataca attaatttta aaatatctat
    52561 tgaaaataga tttaatttaa taatataatc aaaaaaatgc ctgtttttta ttcctgtaag
    52621 aagaattgta aaatttaata ttcatttaag caatcgtttg gctattataa acagctcctc
    52681 tacaggatta aaatccggca aagttcaaaa gattggacca taagcagggc tcaaaagtat
    52741 taaccaacat atttgcagta agaagaaaaa agctcatagg aaggagaata aaatgggata
    52801 tgatatcact tatcatccaa taaaattaga gcaaatggag gaatggtatt ttagccgctt
    52861 agacgaattg aagcaaggcg attattccaa aacatgggaa atggctcaga aggcaaatat
    52921 gcatgaattc tacatagata aatatctgga tgtgatgaaa gaagcagtaa acatagagcc
    52981 tgatctgaca tttgaaataa cgcatggttt taatatcgct gttgtgcaag gttttttcct
    53041 tcctcattat tatgttaggg gaacagcctt ttcattttta attgaagaac atccacaaat
    53101 ggcaaaatat ataacttcat ggcaggatat cataccccag tggataaaaa atccgatagc
    53161 agggggaatc gttgaaaatt actgtggtgg agtattcata tcacctgaac aggttcataa
    53221 actgctttcg gattacgaaa aggacattca gataaaaaat cttattgata acttctttgg
    53281 agaatattta ccagtattca taaaagcatt aaattattct ttggaaaatg gtttaggatt
    53341 gttggaagca acggatgttg ttgtgccaaa tgtgattgat cttaacaaaa cagaatctta
    53401 ttccaatttg tttaattgtg atcctgaagg tgcattaatt tatcgggaga tagccatgga
    53461 acaaattgca caggctttaa aagaaagcgg tgataacaga gatgtaaatg aagcattaaa
    53521 ggatatggcc ttcatgatat cagaacgtga tgaggatgga aatgtagaag attaccgcat
    53581 atatgaacat ccagaaaaag attcttagat aagatggaca tataaaaaac ttataaaaat
    53641 ttttagagac cgtaaaggtc tcttttttct aaaataatat cattggacgg agttaataag
    53701 atgatgcatt aaatggacaa aacttttcga acgacaatat ttgactataa gattacatgt
    53761 atcaaatcat gtttacaata taaaagcaat atatttttta ttttgagata gtttaataaa
    53821 gaagaataaa aaataaacaa ataataatta tgccttgaat ttattaaaag aatagatgaa
    53881 aataaatcta acagaaaaaa gtttcgaaag gaaacaaaag aactgtctta gtatttcttt
    53941 tgtttcaggg gaggttttat gagtaaaatc gcagttgttt acaaatcaaa atatggcagt
    54001 agcaaaaagt acgcagaatg gattgcagat gagcttaagg ctgaactttt tgagcattcg
    54061 aagattaaaa aggatgattt attaaagtat gataccatca tttatggcgg gggtctttat
    54121 gcaggtagta ttagtgggat atcgctcatt actaaaaact ttgataagtt aaaagataag
    54181 aatctgattg tttttacagt gggtacggct caaacggata atacagaagt ttttaagccc
    54241 atcatcgaaa aaaattgttc agaagaagtc agaagagcgg ttaagttttt tcacttccga
    54301 gggagcatgg attataagaa tatgacattg acacataaaa tgatgatgaa gatgttaaaa
    54361 ttggtcttag aaaagaaaaa agaagaaggg ttatccaagg aggataaact gtttttggag
    54421 acttttgggc agaaagtaga ttttattgat aaagcctata tcagtccatt aattgaatat
    54481 gtgaatgctt taagttaagc atctgttaaa cagatgcatt ttgttttttc tatcctaaat
    54541 ctttttaagg cttttgtcga aattaatagt agtaagattt aggggagata gtatgatagg
    54601 acaagtacaa agtaaagagt taattcttga tcaaaatatg cttgatttaa aattttctat
    54661 gtcccttgac tgggagcagg gtgagcttct ggaaggggat attatttcct atggagaaaa
    54721 taaagtaacc gttgaaattg aaggcgtaaa attagatatt aaaagcagtg aaaaaattgg
    54781 agaagtcgga gataaagtta aatttttagt tgaaaaggtt acagaaaatg aactggtatt
    54841 aaaagctttg acagacaatc aggaaattcg tcctattaag caatacgacc gaatccagtc
    54901 tgttttgtta aaggaagtta tccccatgac ggaacaaaat attgaacttg ctaaaaagat
    54961 gcttgacagt ggtattcaag ttgaaaaaga aatttttgaa aaggttgctc aagcaaaaag
    55021 acaaattgaa cagattataa ataccatgac cagagaagag gtaagagaac tcctggaaag
    55081 cccctatgac gccgaaaaga tttcaattga tattatttca agatttcttt gcgaaaaagg
    55141 caaattcagc aaagccatca gcagtgaaga gttagaaaag gaattaaatc aatatgttgg
    55201 tgaaaaaact gaaaaagtta aagttgctat gaaagccttg cttgaaaaag agcttcctgt
    55261 tacgagaaaa aatatagatt ctcttgtttt ggttcaggaa aaactggaag ccataaaaaa
    55321 tatggatgat aaggccattg aacaggttgt aaaaagaaaa ttgccttata cccttaatca
    55381 tttatatgct gcaatttttt ctgccggaga agaagagatc cagatagaaa gtaagcttcc
    55441 ctatggagat gcagggctta taaatacagg agaacatgaa tttagccagg atgaaatcat
    55501 taagctttta aaacatcagg aaatgcctat agatgaagaa cacatcgaag cggctaattt
    55561 tcttattaag cataagctgg aagttaataa agaaaatctg gaagcagtga ttttattaaa
    55621 gcatgaaata aagaaattag atgaaaagat tgtattggat aaagccttaa aatttttaaa
    55681 gcttaacagc aatcctggaa aactggagtt atttgaggat atctataaaa aagataagtc
    55741 attaactgaa aatgaaatta agcaattggt cagagatata aaagttatgg atgatgaggc
    55801 cctaaaaagg gttatcagaa aaaatctttc tattaattta aggaatttac aaaaagaatt
    55861 aaacagcata gcagaagaaa aaaatgttga tgaagaaagt cctttagaat tagaaaaagg
    55921 ttctcaattt atcaatgcga agaggcaact ggaagaggtc cgcttaaaaa tgaccctgga
    55981 agcggccctg aaattaaatg aaaaaataaa gattgataca gtgcctctta aagaattggt
    56041 agaagaactt aagagcttag agcaggaata ctaccgtgaa gcccttcata aggcaggagc
    56101 tgaaagcagc aaggagaacc tggagcagtt agaagcgtta tataataagt tagaaacgat
    56161 taataaatcg ggcagaatgg ttttagctaa ggttgtaaaa aatgaaatcc cacctgttat
    56221 tgatgattta tataaacaga ccttgcaaga aaaagaggtc atcacaaaat atgagcaaat
    56281 ggaaaccagg gtggaaccaa ggcttggaga tgttataagg aaaatagaaa agcagattgc
    56341 acctttatta aaagaacagg ggatagagcc tacggaagaa aatatccgat gtgcccggat
    56401 cttgattcaa aataatatgg aagtcaatga agaaaacatt ctgcaggtaa gagtattaga
    56461 tgagaaggtt aattttatca gtaaaaatct ccatccgatt attgctacta atatgattaa
    56521 ggaaggcttc cgcccggact ttattccaat tgatgaggtt attgactata ttgaagtttt
    56581 tgatgagctt ctgggaaaag atcctaaaga ggccttgggt caatttattc tggagctcga
    56641 tgaaaaaggt gctttaagtc ctcaagaaag ggaagggctt attggtattt accgtatgct
    56701 tcatgttatt gaaaagagta atggaaaagt cataggctgg ctgatgaaaa acaatatccc
    56761 tttgactttg aataacctgg ttgaagcagc taagtatctg gacagaaccc gtggtgtcaa
    56821 gcaaaatatg gatgtccttg tagacgatga ttttggcttt tctgaaaaag tgatttacca
    56881 tgaaaaaagt atcagggcac aattagaaaa ggctttcgga gagaaagtca cagattcttc
    56941 aagattcctt aattttgaca gtatcagtaa agagacaaca gaacaacttc tggattcttc
    57001 gagtttattt aaactggatt attatgaatt gatattagat gaatttgtaa gaaatatgtc
    57061 ttctgaaaag tttatagaat ggctcagtaa aaatccagat ttatatgaaa tggatttaga
    57121 aagcctttat gaagaatata aaaaagaaga tttaattaaa gagggcaatc tttccaaaac
    57181 tccagttaag gaagagacgg tagaagaatt tacaaagacc ttgcaggtca ttaaaaaagt
    57241 taatcaagaa accttaatct ttatggaaaa aaatcagatt cccatgaatc ttaaaaactt
    57301 acaattggtt tctggtctgt tggaaaatcc gcataagctt ggggaagaga tagaaagctt
    57361 ttctaatctt ttaaaagaaa cagactataa agatgagctg catcaggcca taagagaaat
    57421 caatgaaaaa ttaaaacaag gaatggatcc tgaagaggct caggatgaac tcaaagaaaa
    57481 aataaaagaa ataaaagggc agttgttaga atccctcttg ccagttaaac aaacggtctg
    57541 gaagacggga aacgatattg aaaaaatgat agatatcgga aagcagttgc aaaagcagga
    57601 cggcttttac caaataccag ttatcattaa tggtaaggcc gccaatatga atgtgtattt
    57661 acttaaggac agggataaag gagctcttgc aaaaaaagaa agtatcagtg cttatgttgc
    57721 tattgaaaca gagcatcttg gtcaggtccg tatggtcatt catatggatg aaaaaaatgt
    57781 agactttaaa atcagtgggg aaagcaaaga agtaacagaa tacttaaaga gtaaagagca
    57841 tctttttagg gcttctatag agaacatagg ctataaagtg ctcagaagcc aatttaaaga
    57901 agaacttaaa gaattagttc aaagctcaaa agggacttct cttgtagtca ataagaagaa
    57961 acatctggat tcagattttg aaatggttgt ttagggggtg actcatggag gaaaaagaaa
    58021 ataaaaaaat accctcatct ttattggaag tcgttttaga aatgatttct acgatagacg
    58081 aaattgacaa agaaaaagga aatcaagaaa aaacattaaa ggattatcga aatccgtcga
    58141 taaataagtt agagaaaata ataaaaaggt aggtgtgtat acatgaggat taaccataat
    58201 atcccagctc ttagggcact tcatcaatta aataaaacca atacgacgtt ggataaaacc
    58261 atgcaaagac tttcctcagg tctaagaatt aattgtgctg ctgatgatgc agcaggttta
    58321 gccattgcgc aaaaaatgga tacacaagtt cgtggacttc atcaagctaa ccgtaatgcg
    58381 atggacggta tttccctaat tcaaactgct gaaggcgctt taaatgaagt tcatgcaatg
    58441 ttgcaaagga ttagagaact tgcggttcaa gtatctaatg gaacttatga tgcaaccgac
    58501 agaagagcgg taaatgatga agtggttcaa cttcaaaatg aaattcaaag aatttcagaa
    58561 cacattgaat ttaatgaaag aaaacttctt aacggggaaa ttgacagaag agctttcccg
    58621 gataatgaag gtgttgtaaa gattatttct ttaagtgata cggttagtcc tggaagttat
    58681 aagatagaag taacagcgga tgcaaccaaa gcaaccattg caggagattc tggaaaaatt
    58741 aacgcaggcg gcaaaatttc tgatgcccag gtaggaacta ttaacctaaa cggggaagaa
    58801 gtgcgcatag aaaaaggaga tacagctgac gttgtattta ataaattaag agaccttgca
    58861 gaaaccattg gagcaaactt aattgcatat gcggataatg atggaagtcc tgattatgcc
    58921 agcggggctc agcaatacaa ttttgataat atgcatttag tatttgaaat gaaagaatat
    58981 ggttcagaat acaaattgga tttgaatgca tctaacagtg ccttgctatc tgcattaggt
    59041 ttatccacaa cggttcaaca aggaacggat gcacaagcaa aaatcaccag tactgcagat
    59101 ggattctcag caacagcaac cgtatccact aaaggaaatg ttataactgt tacagatatt
    59161 aacggatttg aaatgatgtt tgaagcacaa caaggtgtag cggctttaac agacagtact
    59221 gctaatgtta aaactgctaa tgttaaagta ctggatgcag gtccattgga tttacagatt
    59281 ggtgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcta atgttaaagt
    59401 actggatgca ggtccattgg atttacagat tggtgctaac gaaggacaat tcatggaaat
    59461 tcgtattcaa aacttatcac cacaagcact aggtattgat aagattaatc tgtctacttc
    59521 tgatggtgct caaaaggcta ttaccgtagt agacaaggct attaatatga tttcttctgt
    59581 acgttctaaa cttggggcat atcaaaaccg ccttgaacat actgtagcaa acttaagtgt
    59641 agctgctgaa aacatgacag cttccttatc cagaattcaa gatgcggata tggcagctga
    59701 aatgtctgaa tatactcaaa agaacgtgat tgcacaagca ggagttgcaa tgctggctca
    59761 ggcaaatcaa aggccgcaac aaattttaca attattacaa ggataagggg ataggccatt
    59821 atggaaaata gaactgcaca aattgccaat gcaagtcctg gtcaattggt tgtgattacc
    59881 tatgaattaa ttctggaagc attacaagag gcacaggatc aagttcctgt aaaagatgaa
    59941 aaaaagttta aaagaaccat tgacagagcc cagaaattac taaaagaatt aatggtttcc
    60001 cttgatttat cttacgagat ttcttatgat ttaatgtctc tttattcata tattaataaa
    60061 accatcatta aaggttatgt atctcagaaa aaagaacctc taatggaagc aagcaagtta
    60121 ttaaataccc ttttagaggg atggaaacaa gctgtggctg cagaagggga taatgcccca
    60181 gtgattgaaa atgcacagca aatttatgca ggtcttactt atggaaaagg caatttaaat
    60241 gagtcagtca ttgaagatgc caataggggc tttaaagcct gagacaatta tagaaccggt
    60301 aactctttta taagagttac cggtttcttt tatgaataaa attaagaagg ctggactata
    60361 cttatagtaa ccaaacttat ccaagggagg tatagcctag tgaattttaa tatagagact
    60421 gaaaaacgag gttttttccc ttttcgcttt aaatctaaac aggaagaggg cttaaaagaa
    60481 gcagaagagc ttaaagaaga acttcataaa attaaaaagg aactggattt agcttacaac
    60541 aattttgaat atgccacaga aaaagagcta atcgacagtt gtatatataa aatgatggca
    60601 cttcagataa aatacaaata ttttttagat aaagtcaaag aattggacgc ttctttcgca
    60661 aaagaatggg aacagacctc ttaaggattt taaaatttat gggggattga catgggaagt
    60721 aatgaatttt taatgacatt aattattatt ggttcagtta tactggttct tatgatctat
    60781 ccaaaacctt taaaaatttt attaagaatt atcattcaag ctttaggggg tattgcagga
    60841 ctttatattt ttaactttgt tctgtcgccg cttggatggt ttgtagggat taattggacg
    60901 accatattga ttattgggat attaggtatt ccagggttaa tatttttata tcttttaaat
    60961 cttattttat aaattcctgt gaaatatata at
//
LOCUS       DAOSUD010000003        57665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_381_length_57665_cov_23.314546, whole genome shotgun sequence.
ACCESSION   DAOSUD010000003 DAOSUD010000000
VERSION     DAOSUD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 57665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57665
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_57665_cov_23.314546"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="spoIVB"
                     /locus_tag="PLG49_00895"
     CDS             <1..822
                     /gene="spoIVB"
                     /locus_tag="PLG49_00895"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOQ15896.1"
                     /translation="KTGDLILKVNEKSIENKEELISAIENSNGKVLKMTIKRDNELLE
                     IYVTPIKSEIDKSYKIGAWVRDETQGIGTITYYNPKSYTFGALGHGITDIDTRKIIPI
                     KNGKIMKAEITSIKKGRRGIPGELTGAIYDTEEAQLGTIELNTSQGIFGKLDPKKMKA
                     FPKKSFPIAFQNEIHEGKAIILSNISGDKIEEFEIVIQKVSRFQQDISKGMIIKITDP
                     RLLDKTNGIIQGMSGSPIIQDEKLVGAVTHVFVHDPTKGYGIFIENMLKNESDLE"
     gene            996..1796
                     /gene="spo0A"
                     /locus_tag="PLG49_00900"
     CDS             996..1796
                     /gene="spo0A"
                     /locus_tag="PLG49_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOQ15897.1"
                     /translation="MNTDKIKIVLADDNKDFCEILAEYLNRQSDMEVIGTANNGTEAY
                     EMILEKTPDVVILDVIMPHLDGIGVIEKLKKVQMEKRPVFVMLSAVGQDKITEKALNL
                     GAEYYIIKPFDMETLINRIRQLKGHHEIIKSRGSTMSLSKGSYVPTAHNLETEVTNII
                     HEIGIPAHIKGYQYLRDAIIMSINDMEILNSITKQLYPSIAKQYNTTPSRVERAIRHA
                     IEVAWSRGKMDTINKLFGYTINNGKGKPTNSEFIALIADRLRLELQVG"
     gene            1978..2955
                     /gene="steA"
                     /locus_tag="PLG49_00905"
     CDS             1978..2955
                     /gene="steA"
                     /locus_tag="PLG49_00905"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOQ15898.1"
                     /translation="MSIVGVAKIDSSTKKLIHRLTPKDIAIIDHEDLDYVCAYALASK
                     KVKAVINKSSFSSGKYPNRGPYILLEHQIPLYENIDHSIFKYIKENDKIEIKDNLLYK
                     NEIAVPLKSISKRDCDQFQKKSLNNLKNEMFAFIDNTLYYMKKEKGKLFHPPRLPAIN
                     IPVAHRPVLLVTRGEYYIEDLMRLENFIKKQQPVLLGVDGGADGLAFLNYIPDIIFGD
                     MDSVSDESLKKAKNIVLHSYDNGICPGEARIKKLGLPYSKYSCFGTSEDGAILMCYYM
                     NASYIVIVGSHNNLIDFLEKNRKGMASTVLTRILVGSKLIDAKGFFKLF"
     gene            2971..3678
                     /locus_tag="PLG49_00910"
     CDS             2971..3678
                     /locus_tag="PLG49_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ15899.1"
                     /translation="MNEKISIIIPVYNEEDFIKDTLKGLLEADIKNLGEIIVVDDGSV
                     DETKKKVCEIKSAFIYYYSLARNRGKGAALRKGLELSRYPVIVFLDGDLGYSSKEIKK
                     LLKAIDDKSVDFVIADFPPPLHKGGFGFVKKLSQKGVYFLTGQKIKNPICGQRIFRKE
                     ILADIAIPDGFAVEVGMLIDLLNHGFRVREIPVFMRHRESKRDLKGFIHRGKEFIQIL
                     QILIKKYFQYKNPWNRW"
     gene            3719..4501
                     /locus_tag="PLG49_00915"
     CDS             3719..4501
                     /locus_tag="PLG49_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15900.1"
                     /translation="MSSFFWRDIILDFLIKYNFKKVNYRNKKVPVGMGILLFFSTLIA
                     AYFIIFFTEQAFIYYLFLFGLSLIAFAGILDDSIDEKEVKGLKGHLRKLFKGELSAGA
                     IKALAGIFAAFYIAFLLSGTLSDLILNLLLILFSINIINLFDLRPGRALKVFFLMSAI
                     IWLIGDFSDKYLTFLLIGSSFPIWKGDLKEQYMLGDIGANILGYSLGFTAVISLTPYY
                     KMLSIGLFLFLHLIAEKKSLSEIILKVPFLHYLDNLGRDKIQ"
     gene            complement(4521..6287)
                     /locus_tag="PLG49_00920"
     CDS             complement(4521..6287)
                     /locus_tag="PLG49_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOQ15901.1"
                     /translation="MALDGLVISNIVYEIKKLMLAGKIDKIYQPESDEIILQIRGNGA
                     NYRLLLNSNSNYPRSHITNIQRKNPLSPPMFCMLLRKHLSGGKLIDVVQPDFERIIEF
                     HIENINELGDLTVKKLIIEIMGRHSNIILTDEKNCILDSVIRVSKDKSSVREVLPGKI
                     YQRPPSQDKINPLSLEEADFVTQLKGNKGTKLQQTIYKTFTGISPLVASEICHRANID
                     VSAYLEELRDEDLKRLSKVMNGIFKQVKANLFQPQIIYDPHTGEPVEFSSIDLSQFTG
                     YAKKYYDSVSEAIEDYYKVKDRLSRIKEKSRNIHKIIQNNLERCYKKKEIQIRQLQEV
                     SDCDYLRVFGELITSNIYSISKGMTSFTTVNFYDENMTELTIPLDPVLTPAQNAQYYF
                     NKYNKAKRTKNAVQEQLKRVNEEISYLESLLSSLEASTDIEDIEDIKKELQEEGYIKN
                     KKGKSSNEKVKSKPLHFKSAEGFDIYVGKNNRQNDELTLHSAPTDLWLHTKDIPGSHV
                     IIKTENKEVPEETLLLAAHLAAYYSKARNSSNVPVDYTLRKNVKKPRGAKPGMVIYEN
                     QKTLYITPDEIKIKQLISKSIQ"
     gene            6470..7348
                     /locus_tag="PLG49_00925"
     CDS             6470..7348
                     /locus_tag="PLG49_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOQ15902.1"
                     /translation="MIRSMTGYGQGKLISDDRKFTVEIRSVNHRYNDINVRLPRTLNY
                     LEDKIRTFIKERVSRGKIDVFVSFETTSKDDFEVYLNENLLDAYLEQLNIIKNKSTVI
                     DDISVSLLARFPEVIIVNKKEDDKDILWQLLEEALKEAFNDFLAMREKEGENLKEDLL
                     AKLKVCENYLAKIKERSPNLAEEYKNKLEKRLQELLPDHSIDENRLAMEIAIFADHCS
                     IDEEIVRLESHINQFRNILNHEEIVGRKLDFLVQEMNREVNTIGSKANDLQISQSVIE
                     LKSEIEKIREQIQNLE"
     gene            7369..7644
                     /locus_tag="PLG49_00930"
     CDS             7369..7644
                     /locus_tag="PLG49_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOQ15903.1"
                     /translation="MGIKLINIGFGNIVSANRIISIVSPESAPIKRIIQEARDRGMLI
                     DATYGRRTRAVIIMDSDHVILSAVQPETVANRLTSKNQDSLNTEIDE"
     gene            7660..8268
                     /gene="gmk"
                     /locus_tag="PLG49_00935"
     CDS             7660..8268
                     /gene="gmk"
                     /locus_tag="PLG49_00935"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOQ15904.1"
                     /translation="MNNTKGIIVVVSGPSGSGKGTIVSELIKNEQYALSISATTRKPR
                     LGEEEGIHYFFKSKEEFMDMIEKRELVEWAEFCDNYYGTPKFYVESKMNEGKDVILEI
                     EVQGALQVKKLYPEAVLIFIVPPSLKELKNRLINRGTESSEIIEKRLRRALEELEFIN
                     DYDYLVINDCIEQAVMDINTIIKAEHLKVNKRMSLIEKFKEE"
     gene            8274..8582
                     /gene="rpoZ"
                     /locus_tag="PLG49_00940"
     CDS             8274..8582
                     /gene="rpoZ"
                     /locus_tag="PLG49_00940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOQ15905.1"
                     /translation="MLKPSYSDLIEKLNEKSSDEEKPIITSRYSIVIATAKRAREIVD
                     KGDPRVESKSNKPVSVAVEELYQGKTIIKYNESKTGHNVEEIEEVFEEDVKASDNEDN
                     "
     gene            8682..9890
                     /gene="coaBC"
                     /locus_tag="PLG49_00945"
     CDS             8682..9890
                     /gene="coaBC"
                     /locus_tag="PLG49_00945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOQ15906.1"
                     /translation="MMNKTVVLGVTGGIAAYKACEIASRLVKSGIEVNVIMTRSAQEF
                     VRPLVFQSLTNRPVATDMFKEPVTWDIEHISLAKKADLFLIAPATANIIGKIAAGIAD
                     DMLSTTVMATKAPVVIAPAMNTAMYNNVIVQRNIQTLSDLGYHFISPAEGRLACGDTG
                     IGKLADVDIIVEKVLELLDQQPQDYKGKKVVVTAGPTREALDPVRYISNHSSGKMGYS
                     LAEAAVKRGAEVILISGPTHIKPPCGCQTIFVTTTEEMYQEVIKHYENVDVLIKAAAP
                     SDYTPEFVSDHKIKKNKEGLTIKLKLNKDILKELGRLKKKQILVGFAAETDNELEYGK
                     KKLEEKNLDMICINNVAQENVGFGYDTNKITLVKKTGEEKSLPLMSKKELANMILDEI
                     KDLLMEQDHI"
     gene            9903..12377
                     /gene="priA"
                     /locus_tag="PLG49_00950"
     CDS             9903..12377
                     /gene="priA"
                     /locus_tag="PLG49_00950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ15907.1"
                     /translation="MIAEVIVGISHEKLDKIFHYIVPKELESKAQIGVRVLVPFGKSN
                     KKIEGYIIGFCENSSIETDKLKELYSIVDKSPLFNDHMLALAKWMREYYGANMVDCLR
                     CILPSGLKIKAQTFVRLNPDFKNPEVLESEKRTEKEKEILLYLLNKDSYVLMEDLLDL
                     FGGQIKKELKKLRDEKLLEVREKELIKDYKCVIKYVSLHKEMTMEQLENILMEYQSKK
                     NYRAQCRIIEFLMMNETIPLNEMKKILEVSSPSINALLKKDIIEIEEIEVSRDSYRHL
                     YNEKTEAFTPTFEQANVIRYVLNKIDSLKGGKILLHGITGSGKTEVYLQLIEEVIKRE
                     GQAIVLIPEISLTPQTVQRFKSRFGEKVAVTHSKLSLAERYDQWKRARDGEVSVMIGP
                     RSAVFTPFSSLKLIIIDEEHENTYKSEFAPKYHAREVAEKRCELTGAVCLIASATPSL
                     EAYYGAKTGIRDLLELTYRANNSSLPQVEIVDMRNELEEGNRSIFSNYLKEEMGKKLK
                     NKEQIILFLNRRGFSNFVSCRKCGYVLKCKSCSISYTYHAYNHSLVCHYCGDRILSPS
                     VCPQCGSKHIRYFGVGTERVENEVKKLFPEASVLRMDLDTTKGKYGYEKIIEQFRTQK
                     ADILIGTQMVAKGHDFPNVTLVGILAADLTLHIQDFRSSERTFQLLTQVSGRAGRGDL
                     PGKVIVQTYDPEHYSIVAAKNHDFLTFYNQEISIRKELDYPPFTHIFTVLLVASNEKE
                     VIQSAYKLMEIMKYYNRKGNFKLYGPVPAMISKIKNKYRWRIIIKCNDRNLLMQYAFY
                     CIDQYKKKFDNNQLLIQTDIDPLMIY"
     gene            12396..12857
                     /gene="def"
                     /locus_tag="PLG49_00955"
     CDS             12396..12857
                     /gene="def"
                     /locus_tag="PLG49_00955"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOQ15908.1"
                     /translation="MALRQIRTDGDEILRKKAKKVEQITPSIITLLEDMVETMYNADG
                     VGLAAPQVGVLRRVVVIDIGEGVIELINPELIETEGEQIGPEGCLSVPGVTGEVERPL
                     KVKVKALNRKGEEFIIEGEGLLARALCHEIDHLDGIIFTDKVIRYLDEEEE"
     gene            12874..13830
                     /gene="fmt"
                     /locus_tag="PLG49_00960"
     CDS             12874..13830
                     /gene="fmt"
                     /locus_tag="PLG49_00960"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ15909.1"
                     /translation="MKVVFMGTPDFAVPTLQKLIDEKYEIAAVVTQPDRPRGRGKKTL
                     APPVKELALQYHIPVYQPEKVKTPEFIDSLQKIHPDLIVVVAFGQILPKAILEMPQYG
                     CINVHASLLPKYRGAAPIQWSIINGEEITGVTTMFMDEGMDTGDILLKKEVKIYPEDT
                     AQELHDRLSVEGASLLIETLKQLQTGQLIRQKQNDQEATYAPMLQKEMGCIDWSKSSK
                     EIINLIRGLSPWPSAYTYYKDQMIKVWKAEEYHEKYDAHVMGEITEIIKDKGIVVKTG
                     DGSLMITEIQAENKKRMSVVDYLRGHKIEKNIVLGNNRNEIK"
     gene            13845..14534
                     /locus_tag="PLG49_00965"
     CDS             13845..14534
                     /locus_tag="PLG49_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOQ15910.1"
                     /translation="MYIDTYYIIFVLPAILLTIYAQARVHSTFNKYLKVRNLRGMTGA
                     EAARIILQNQGIYDVTIERIPGNLTDHYDPRTKTLRLSGPVYDGTSIASVGVAAHEAG
                     HAIQHNESYSFLKVRHSIFPVVSISSRMAIPLIMLGFVLSFSFLVELGIILFCAVVFF
                     QIITLPVEFNASKRALVALEGQGILSDNEIRPARKVLSAAALTYVAAAAVAVANLLRL
                     ILLANGGRRRR"
     gene            14655..16004
                     /gene="rsmB"
                     /locus_tag="PLG49_00970"
     CDS             14655..16004
                     /gene="rsmB"
                     /locus_tag="PLG49_00970"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOQ15911.1"
                     /translation="MEKLKPREIAVNIISEINEEGAYSNISLKDNLSYYGYLTDLDKA
                     FITNIVNGTVRYLLTIDYVINSFSKIKTKKMKAIIRSILRASVYQILFMDKVPPSAAC
                     NEAVEITKKKGLSGLSAFVNGVLRNIVRHKDMISYPDPKKDFLNYLSVRYSFPQWILD
                     YWLQYYEADFVEELCQASNINPPISIRCNLLKNDISSLAKSLLKEGVQVSQGLYLKEA
                     LYISKTSSLGDLESFNKGLFQVQDESSMLVGHILAPEKGERILDVCSAPGGKATHLGE
                     LMHNEGYILARDIHEKKLNLVADTAKRLGICIIETEEKDATELDEKLVNTMDKVLVDA
                     PCSGLGIIRKKPDIKWKKENRDLESLINIQRKILEISSQYLKRNGVLVYSTCTISEKE
                     NLENIKWFIKNFDFELEDLNPYIPESIQNHDTKKGYIQLYPHIHNTDGFFIARLRKK"
     gene            16014..17063
                     /gene="rlmN"
                     /locus_tag="PLG49_00975"
     CDS             16014..17063
                     /gene="rlmN"
                     /locus_tag="PLG49_00975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ15912.1"
                     /translation="MQDREIASLTVEELKKLMLEMGEKEFRGEQIFHWIHGKRTVAFD
                     EMTNLPGELREKLKASFDCNLVSLEQKYISKIDKTIKYLFRLKDNNIIESVLMSYNYG
                     NSACISSQVGCRMGCAFCASTLEGLIRNLSAGEMAGQIYQIEKDSNRKISNIVIMGAG
                     EPLDNYDNILKFISIINHPKGANIGQRHITLSTCGLVDKIYDLADKNLQITLAISLHA
                     PNDRIRQEIMPIAKRYYMDELLKACSYYIEKTHRRITFEYALINQVNDREEHAYELAD
                     KLKALLCHVNLIPMNPVKESHYRESHQERINYFKKILESKGIQTTIRRRLGSDIDAAC
                     GQLRRRYLEGNGVEK"
     gene            17060..17809
                     /locus_tag="PLG49_00980"
     CDS             17060..17809
                     /locus_tag="PLG49_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOQ15913.1"
                     /translation="MISAGQCHIGRHRNKNEDAIFLHDQPYGCLPNLYIVADGMGGHQ
                     AGEYASSLAIQCFCEFVREHRDHEITRDNLNAFLHDAILYTNNHVYSKAQNDPNYNGM
                     GTTFLVAVFLEDKIYVAHVGDSRVYLIQGDRIRQLTLDHSFVEEMVRKGEITEKEALH
                     HPNRNIITRAVGTNSTVDVDFYTEDITDETLTLMCSDGLSNMLDEEEILHIVKGNADL
                     EDLSQALVDRANEKGGFDNISVIIIRGREGR"
     gene            17814..19862
                     /gene="pknB"
                     /locus_tag="PLG49_00985"
     CDS             17814..19862
                     /gene="pknB"
                     /locus_tag="PLG49_00985"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOQ15914.1"
                     /translation="MLQKGYILSNRYEIIEKIGAGGMSIVYKAKCNKLQRYVAIKVLR
                     EEFVSDEEFVTRFRVEAQSAASLSHPNIVNIYDVGNEDNLYYIVMEYIQGKTLKQMID
                     EEAPFSSTKTLKFGLDIASALQHAHKNNIIHRDIKPQNILVTDEGVLKVADFGIARAV
                     DSSTVVTTGNAIGSVHYFSPEQARGGFVDKTSDIYSLGIVMYEMITKTLPFEGDSPVT
                     VALKHINEELPDPSTVNPDIFPSLEDIILKATQKKTENRYKSIDEMIRDMERAIDEPT
                     GKFVYMNQRDLIDSPTIEMTQDEMKILRGEYQNNYSKELNGLKDDMDAEFEDFDNEDS
                     EDEAFEEKNPKEKWVVAGAVFTSLIIIVILSAVGIKMIRSYLTPKVVEVPSLLNMTVE
                     EAKALLDERKLILIEEEEIFDDEIEEGKIAFQDPQEGTVININSEVVVKVSKGYQSFE
                     VPDVVDLDYDTAIRMLEEYFDVSVDSQYSDEVEENHIISQEPEAGTLLKEGDTVKITI
                     SLGKEIQTIPVPKVVNLSEEAAVNQLRNSGLNVGSRTYVPSDEVEKGYVISQSVAPGE
                     LVREGYAVDLVISEGSNKTEEPDKPTDNNNTGGPVEKVIEIMAPTDIDLPIVNVKIRL
                     NRENRFVYDKNHDISEFPIKVPIVGEGEGFIEVYINDVIKYRENISFTNPVNTVEETE
                     "
     gene            19862..20746
                     /gene="rsgA"
                     /locus_tag="PLG49_00990"
     CDS             19862..20746
                     /gene="rsgA"
                     /locus_tag="PLG49_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ15915.1"
                     /translation="MVGTIVKGIAGFYYVRVDDKVYECKAKGKFRNINLIPYVGDKAE
                     ITLLNEENRGSIVNILPRKNYLIRPPVANVDQAVIVFAVKNPNVNLQLLDRFLILAEE
                     QGLEIVICFNKIDLEEEKVCKELAAIYKKAGYKTILSSTVSNEGILELKEALADKTTV
                     FAGPSGVGKSSLLNAIDTSLKLATGMISEKIKRGKHTTRHVELLPLDNGGFVLDTPGF
                     SSLSLSHLDSMKLQDYYREFVPYNNACKFIPCSHIHEPQCKIKEMVEEGLIDFERYQR
                     YKSLYNELESNEQRRWKK"
     gene            20743..21381
                     /locus_tag="PLG49_00995"
     CDS             20743..21381
                     /locus_tag="PLG49_00995"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOQ15916.1"
                     /translation="MKILIIAGGDIEDYSFARSYADDSDYIICADRGISHARAMSLVP
                     DLIVGDFDSASKEDMHYYQDKNIIFKSFPSKKDKTDTELALDIAIEKNPREIYIIGGT
                     GNRLDHTLANIHLLYYGLQKKIAISLITSTQKIYLIDKEITLKGEKGNFVSLIPFSLE
                     VHGVTTKGLEYPLNEAVLYSGSSYGISNVMTASTASICIKAGFLLVIQVLKE"
     gene            21471..21614
                     /locus_tag="PLG49_01000"
     CDS             21471..21614
                     /locus_tag="PLG49_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15917.1"
                     /translation="MKSRILGLFLFSVGMGMLLVFLLPGTSFVVITAILFLFLGYIFF
                     KKY"
     gene            complement(21741..21932)
                     /gene="rpmB"
                     /locus_tag="PLG49_01005"
     CDS             complement(21741..21932)
                     /gene="rpmB"
                     /locus_tag="PLG49_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOQ15918.1"
                     /translation="MAKCDVCQKGVHFGIQLSHSHRRSNKQWKPNVKKVKVLLDNGTK
                     KRMNVCTRCLRSNLVTRAN"
     gene            22118..22474
                     /locus_tag="PLG49_01010"
     CDS             22118..22474
                     /locus_tag="PLG49_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOQ15919.1"
                     /translation="MSGKIVNEYGNIFIDNEVIARIAGLAAIECYGIVGMAIKNVTDG
                     LVQLLKRESLTKGVKVIVEEDAVSIDFHVIVEYGTKISAVADNLISTVKYKVEESTGL
                     EVKKINIFVEGIRVDN"
     gene            22496..24157
                     /locus_tag="PLG49_01015"
     CDS             22496..24157
                     /locus_tag="PLG49_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOQ15920.1"
                     /translation="MNIPKIDGEMLKRMIIAATNNLGKNKRMVDELNVFPVPDGDTGT
                     NMYLTILSAAREVEKTESKDVFEIAKAAASGSLRGARGNSGVILSQLFRGFSKGLQGY
                     SVADTKALANAFDSGVKTAYKAVMKPKEGTILTVARGMAEKAQALAEETDNNNIESFL
                     QDIFAHGHQVLKKTPDMLPVLKQAGVVDAGGRGLLEILEGAISALYISEDISLVDNSL
                     TAKDAGTYSALASVSQEEITFGYCTEFIINCSENNESAADELRQYFNTLGDSIVVVED
                     DEVIKIHVHTNHPGKVIEKGLSFGPLTNIKIDNMREQHSSHLSIEKEESKAKESPSEK
                     KEIGFIAVSAGEGLKEIFKGLGVDYVIQGGQTMNPSTEDILNAVEKVNADTIVVLPNN
                     KNIILAAEQAQKLSQEKRIEVIPSKTIPQGIAAMIGYEPHRTIDDTIESMKESMSYVK
                     TGQITFAIRDTVIGDREIKEGNILGLIEGDIAVVSDDLDKATLELIDKIVDDDSEIIS
                     IYYGEDVDESQTDKIRKYIEQVYPDCEVEIHYGGQPLYYYIFSVE"
     gene            24215..26269
                     /gene="recG"
                     /locus_tag="PLG49_01020"
     CDS             24215..26269
                     /gene="recG"
                     /locus_tag="PLG49_01020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOQ15921.1"
                     /translation="MNLSDSVKELKGVGEQTLKKLNRLDIYTIEDLLYHFPRDYEDRS
                     EIKKIEEITFNEINTIIAQVSALPQNIKKGNLILTKVRIKDETGSIFAVWYGQPYLKK
                     HFKPNDSYIFTGKVLYKYGEIQMESPEYELNNGKEVLSFGRIIPVYPSTYKLSQKLIR
                     QMIYQAIKETKNQLTEFLPKWIRKKYQLADYNYSMLNIHFPQSRESFFIARRRLVFEE
                     LFLLQTGLLRIKNKIKNNKKAIKFEDVEEVNDLIRHLPFELTQAQLRVINEIKKDMLS
                     DRSMNRLVQGDVGSGKTIIAALALYMAVKNNYQGVMMAPTEVLANQHYQSLLSLFEKE
                     NINIGLLTGSLTKKNKELLLKEIEEGRIDIVIGTHALIQEKVAFKSLGLVITDEQHRF
                     GMEQRRLLFEKGKNPDVLVMTATPIPRTLALILYGDLDISIIDELPPGRQKVDTYCVN
                     TSYRERIYRFIKKELASGRQAYVICPAVEESDIEGLQSVIQYTEQIKKVFNEYKVCFL
                     HGKMKAKEKQEIMEAFQKGEIDLLVSTTVIEVGVNVPNASIMLIENAERFGLSQLHQL
                     RGRVGRGKHKSYCILVTDSDNKITKERMKVMTKSNNGFEISETDLLLRGPGEFFGTRQ
                     HGLPDLKIANLYKDMDILKEVQEASKELLSLDPDLKAEEHYLLKEKINKFFEEKTKNI
                     SL"
     gene            26376..26588
                     /locus_tag="PLG49_01025"
     CDS             26376..26588
                     /locus_tag="PLG49_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOQ15922.1"
                     /translation="MASRNRKAVPEAKAALDRFKFEIANEIGVPLKQGYNGDLTSAQN
                     GSVGGYMVKRMIEQQERQMAGNTTLQ"
     gene            26829..27383
                     /gene="rsmD"
                     /locus_tag="PLG49_01030"
     CDS             26829..27383
                     /gene="rsmD"
                     /locus_tag="PLG49_01030"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOQ15923.1"
                     /translation="MIAGVAKGHKLKAPKGMDTRPTTDKIKESLFNIIAPDLYDCNFL
                     DLYSGTGAIGIEALSRGAKKAVFIDRSRICRKIIEDNLNHTKLIDKAVIYQNDVNKGL
                     DILKEKGEIFDIIFMDPPYLDNDIEKILEKISKLNLITPSGYIIVEHSSEKRIQAIAD
                     LWIWKEKIFKTTRMTFLKVVEESL"
     gene            27380..27856
                     /gene="coaD"
                     /locus_tag="PLG49_01035"
     CDS             27380..27856
                     /gene="coaD"
                     /locus_tag="PLG49_01035"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOQ15924.1"
                     /translation="MKIAIYPGSFDPVTNGHIDIIFRASEMVDHLIIGVLSNVKKQPL
                     LTVEERIDLLKATTKYLKNVEVEAFSGLLVDFAAKKNATIIVRGLRAMSDFEYELQMA
                     QTNRSLNKNIETIFLATNIHYSFLSSSVVKEIAMFGGDIHALVPPVVEEYLKSKFK"
     gene            27868..28377
                     /locus_tag="PLG49_01040"
     CDS             27868..28377
                     /locus_tag="PLG49_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOQ15925.1"
                     /translation="MDSILQLLEAIEDMLDSSSAVPFTGKVMIDKDELYEIITDIRLK
                     LPNEIKQSKWVIEERNKILIDAQKEAENTLKEADAKLAKLVDEHEITKRAYEQAEEII
                     EAAKQNAREMRLGALDYADEILAEVEKSLKQALEVIHNQNMELEDSLGRVINTIYTNR
                     QELKGNAQK"
     gene            complement(28385..29578)
                     /gene="ylbJ"
                     /locus_tag="PLG49_01045"
     CDS             complement(28385..29578)
                     /gene="ylbJ"
                     /locus_tag="PLG49_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HOQ15926.1"
                     /translation="MRYKDTLSLSRLITVCIIILLTTGIIIFPQEVLEASKRGIQLWF
                     NTLFPSLFPFMVATYLLIELGVVHFIGILFEPVMGPIFNVPGEGAFPWMMGIISGYPI
                     GAKITADLRCKGKISQVEAQRLIAFTNNSGPLFILGAVSAGMLSKAETGFFLLAIHYL
                     SSLTVGLLFRFYKKEKKVHGKKYKNIIKKSFQQLSLIQKNNSKKIGLILKESIMSSMD
                     TVVQIGGFVILFSVTSQLLELSSLLDLFTKLFKPFFKLIVFSPKLISAFLLGIIEITN
                     GISRIGLCSASLKEELALVSFLIAFGGLSVFMQTVSVISSSDINIPIYACAKLLQGLF
                     AFIYSVTFYPQMTKISAYTQTVFNPISYNITNSFWFKSGPYLCAFICILFILFNIKLF
                     RTKEN"
     gene            complement(29650..30909)
                     /locus_tag="PLG49_01050"
     CDS             complement(29650..30909)
                     /locus_tag="PLG49_01050"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOQ15927.1"
                     /translation="MKVLGIVAEYNPFHNGHLYHLEESKRISHSDFTVVVMSGNYVQR
                     GEPAIVDKWTRTQMALESGADLVLELPLIYATSSAEFFAFGAISILHHSNIVDHLSFG
                     SESGSIDLLSQIASFLFLESEHYKMKLKEYLSEGLPFPSARNKALISDMSDKNTSIPV
                     EELSQVLSSPNNILAIEYLKALKRLNSPIKPFTIKRKGASYHSTQLDTLFASATGIRH
                     ALKNKDHSILYKTMPKKAADLFLEAIHLGKAPVYYDDFSFELQYLLRSMPMEEIKKIM
                     EVDEGIENRINKAAMNHFYIKEIIESVKTKRYTMTRIQRTLLHILLQIYSEDFWELHR
                     HGGPQYIRILGFRKSAAPLLKKLKINSKLPIISNVRRAYRDFSGLPKKMLEKEIQSTD
                     IYSLHYPNLCQRKSGIEFSMPLIIINK"
     gene            31080..32078
                     /gene="pta"
                     /locus_tag="PLG49_01055"
     CDS             31080..32078
                     /gene="pta"
                     /locus_tag="PLG49_01055"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOQ15928.1"
                     /translation="MDFIQTIKQRAKSDKKTIVLPESLELRTLKATEKILKEDIANII
                     LIGKENEILEKAEGLDISGVKIIDPENYAQMNDFIDTLVELRKNKGMTREKAEELLKN
                     PLYFAVMMVKKGIADGMVAGAVNSTADVLRPSLQILKTAPNTKLVSAFFVMVVPDCEY
                     GANGTFIFSDAGLVPNPNSEELAAIAVSSAKSFEHLVATEPIVGMLSFSTKGSACHPD
                     VDKVIEATKIAKELAPDLKIDGELQLDAAIVPSVGASKAPGSEIAGKANVLIFPDLDA
                     GNIGYKLTQRLAKAEAYGPITQGIAKPVNDLSRGCSDDDIVGVVAITCVQAQINEN"
     gene            32188..33384
                     /locus_tag="PLG49_01060"
     CDS             32188..33384
                     /locus_tag="PLG49_01060"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOQ15929.1"
                     /translation="MNILVINCGSSSLKYQLINMVEEKVYAKGICDRIGIKGSVLKHT
                     PAGKDTVMKEVAMENHKDAVREVLSALTHEEYGVIRSMDEIAAVGHRVVHGGEKFSAS
                     VIIDDEVIKAIEECSDLAPLHNPPNLIGIEACRNLMPDTPMVAVFDTAFHQTMPSRAY
                     LYAIPYELYEEHKIRRYGFHGTSHKYVAHRAAEMLNKPIEELKIVTCHLGNGASICAV
                     KNGQSIDTSMGFTPLAGLVMGTRCGDIDPAIVGFLMDKENISSEEVDVILNKKSGILG
                     LSGVSSDFRDIEAAISNGNKRAEIAMEVFVYTVAKYIGAYAAAMNGIDVIVFTAGLGE
                     NNSEVRRDVCKYLDFLGVKVDEEKNRCRGIERDFSAEDAKVRTLVIPTNEELMIARET
                     RNLLKK"
     gene            33447..33962
                     /locus_tag="PLG49_01065"
     CDS             33447..33962
                     /locus_tag="PLG49_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOQ15930.1"
                     /translation="MILNVSKIINNPGSSMELSFDEKIDFIECGKEKIKLIEPVRFVG
                     EISCGDGKELYLTGKLNTRIELPCSRCLQPVEVTIELSLKEYFTKKGNLKADDEEFHP
                     IEGNEIDLGPVFIKDILLNIPMKNLCNAHCKGLCPKCGQDLNKGTCNCKEDVIDERFA
                     VLKTLFNGSNS"
     gene            33981..34157
                     /gene="rpmF"
                     /locus_tag="PLG49_01070"
     CDS             33981..34157
                     /gene="rpmF"
                     /locus_tag="PLG49_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOQ15931.1"
                     /translation="MAVPKGKVSKARKNKRKANWKLTAPNLVKCEKCGELMMPHRACK
                     SCGTYNNKVVVNVE"
     gene            34243..35250
                     /gene="plsX"
                     /locus_tag="PLG49_01075"
     CDS             34243..35250
                     /gene="plsX"
                     /locus_tag="PLG49_01075"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOQ15932.1"
                     /translation="MSYKTVIALDAMGGDNAPSAIVKGAVLAAREDIKIILVGDENKL
                     KTELSQYGMDSSRLSIHHASEVITMDDSPVAAIRSKKDSSMVVGLKLLKEKEVDAFIS
                     AGSTGALLAGGTFLVGRARGIERPALAPLIPNIKGFSLLIDCGANMDAKASYLAQFAL
                     MGSIYMESIMGIPSPKVGLINVGTENEKGNQLTKEAFSLLEKSDINFIGNIEGRDISM
                     GVADVIVCDAFTGNIILKHTEGLALSLFNIIKEELMNDNLSKIGALLSKSAYKRVKKR
                     FDYTEYGGAPMLGLQGLVVKAHGSSNAKAIQSAIAQAVKFKEEKIVEKIEEKINQKIN
                     Q"
     gene            35327..35563
                     /gene="acpP"
                     /locus_tag="PLG49_01080"
     CDS             35327..35563
                     /gene="acpP"
                     /locus_tag="PLG49_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ15933.1"
                     /translation="MVFEKVRQIIAEQLNISESEITEDTSFQDDMGADSLDIFQIIMA
                     LEEEFDIEISNEDAEKISTVKDAVEYIQNMISAE"
     gene            35674..36381
                     /gene="rnc"
                     /locus_tag="PLG49_01085"
     CDS             35674..36381
                     /gene="rnc"
                     /locus_tag="PLG49_01085"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ15934.1"
                     /translation="MEKDNLELIAEFENIIGYKFKNKKWIMEALTHSSYSNENKKKNL
                     TNNERLEFLGDAILDLIISEYLFNLYPDLPEGELTKLRASIVCELSLSTVANKYNFGK
                     YLFLGKGEELTGGRERPSILADAFEAILGAVYIDGKMESAVDFIKRILIPSIKNNQAF
                     RDYKTMLQEEIQKSSHEPIEYSIISESGPDHDKEFFVEVKHSNVTLGKGKGKSKKEAE
                     QNAAYNALNAYQDKKEK"
     gene            36410..39994
                     /gene="smc"
                     /locus_tag="PLG49_01090"
     CDS             36410..39994
                     /gene="smc"
                     /locus_tag="PLG49_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOQ15935.1"
                     /translation="MYLKRLEIQGFKSFADPLKLDFSKEITAVVGPNGSGKSNIADAL
                     RWVLGEQSPKTLRGAKMEDVIFSGTQNRKPLGFAEVSIVLDNSEGKIPIAFDEVRITR
                     RVYRSGENEYFINKSSCRLKDIHQLFMDTGVGKEGYSIIGQGQIDRILSTKPEDRRFL
                     FEEAVGIVKYKHRKLEAEKKLEQEKQNLIRVEDIISELDKQLGPLASQAEKARVYLEL
                     KEKVKKLELNLFIYEADNQKKELDSLEEMFLNNQEELSRQKEIQLKDKENYTALKREL
                     EDIENNIYDNQNSISDIRANIEKTEGNNKVLEQQIEHYILDIKRLNNESEKLDKRIEG
                     LSQEKIELSAKFNALSLELKTKEKLLQEEENHFSGLIHFLLTGESEIENYKSDIIEKM
                     NESSELKTLIQKIDSEIQYIEERKKQILSEKELLDRQYREQSVHLKAIKKLEETLKEE
                     RKKLNQKINELISINNQFIADVQKESTALNDLKEEMHSIKSRKDVLELMENEYEGFNK
                     SVKSLMRLKKDKPNQLKGICGVVAGLIEVPKGYETAIDLALGNSLQNIVTETEEDAKD
                     AIDYLKQNNLGRATFLPLSAIRGKSLGADEQKILNEQGVLGIASSIVHYDELYKDVIS
                     YLLGRVIIVDNLNHAIWIAKKYQHKYKIVTIDGEVLNPGGAMTGGSIQQKSMNIFLRS
                     RELQELKEKLIKLTEEIKNREERIRAYEDKKNQNLAKIEDLKSKIQELDINILSNQHE
                     IDKCEEMLNQLKDKESQLNLEEEQINIQHKENTNSLFEKREEMSRIENEIKAIQSKIE
                     MNQSKLEKEKEAKDILTDKITKLKIEISSLEQKRDYILENKKRLDSEIEKQKAEKKQR
                     EEEIQGILKDKALKENEVNENVREIEKLRQTLIEKTGQQERFQEVKKEKLAVYKALEE
                     KIEESSKTIELLQTEMNRLENKKTKLKLEQEVLYNKIWEEYEETYHSALKYKSTELSI
                     SDMKKLLAKHKNEMKDLGNVNINAIEEYEKVKERYDFLTAQREDILMAEKKLFGIIKE
                     LTASMEKQFIQNFATISANFNQVFTELFAGGKAYLKLTDEENILESGIEIIAQPPGKK
                     LQNMMLLSGGEKALTAIALLFSILKMKPSPFCVLDEIEASLDDANVMRFASYLKKFRD
                     STQFIIITHRKGTMEVADTLYGVTMEEQGISKLLSVKLSDIEENEEAS"
     gene            40007..41134
                     /gene="ftsY"
                     /locus_tag="PLG49_01095"
     CDS             40007..41134
                     /gene="ftsY"
                     /locus_tag="PLG49_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOQ15936.1"
                     /translation="MSLFDKWLNRDKNKGQEESIPEESQLEEFVHDKTEEDIGFSEES
                     YEIKESVEEEENPPEEEGQEESVKEKKGFFSKLVKGLAKTRNNIMDGIDNVLKSFNKI
                     DEELYEELEETLIMADIGVNASIKIMNGLREKVKERKINEPAALKQLLIEEITEILSK
                     GNRDLNIQEGPAVILVIGVNGVGKTTTIGKLAHLIKKENKKVLLAAADTFRAAAIEQL
                     EIWGNRTGVDVIRHQENSDPGAVVFDAIQAAKARKIDVLICDTAGRLHNKKNLMEELR
                     KIFKIINKEYPEARKEVLLVLDATTGQNALQQVKLFKEVTDISGIILTKLDGTAKGGI
                     IIAINSEMDIPIKYIGVGEGIEDLRPFNPKEFAEALFTDEE"
     gene            41222..41578
                     /locus_tag="PLG49_01100"
     CDS             41222..41578
                     /locus_tag="PLG49_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HOQ15937.1"
                     /translation="MEKLLQITLLYDFYGELLTDKQKNIFELYYQNDFSLAEIADYLK
                     ISRQGVYDTLKRTENILYDYENKLKLVERFEKNKTYIKEIVNTINEAEKHLDDLNKVH
                     ICFAELKRIANQLIIE"
     gene            41595..42929
                     /gene="ffh"
                     /locus_tag="PLG49_01105"
     CDS             41595..42929
                     /gene="ffh"
                     /locus_tag="PLG49_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOQ15938.1"
                     /translation="MAFESLSDKLQEVFKKLKGKGRLTEKDVNEALREVKLALLEADV
                     NFKVVKSFINKIKERAVGHDVLESLTPGQQVIKIVNEELTELMGSTQSKLTFSNTPPT
                     IYLMVGLQGSGKTTTSGKLAAQIKKMGKKPLLVACDVYRPAAIKQLQVVGSKVGVPVF
                     SMGDATNPVDIAKAAVSYAKNNNHDLVIIDTAGRLHIDEELMNELYEIKEVTKPQEIL
                     LVVDAMTGQDAVNVAESFNEKLGIDGVILTKLDGDTRGGAALSVRAVTQKPIKYVGMG
                     EKLEDLQPFHPDRMASRILGMGDVLSLIEKAQENFDQKKAQELEKKIRKMEFNFEDFL
                     EQMQQVKKMGPLNQLIGMIPGMNMQKLGNVEISDKEIAHIEAIIQSMTLEERRNPEII
                     NPSRKRRIAAGSGRDIQEVNRLIKQFEQMKKMMKQLSGMTKGKKGKFKLPFM"
     gene            42968..43213
                     /gene="rpsP"
                     /locus_tag="PLG49_01110"
     CDS             42968..43213
                     /gene="rpsP"
                     /locus_tag="PLG49_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOQ15939.1"
                     /translation="MAVRIRLKRMGAKKAPFYRIVVADSRAPRDGRFIEEIGYYDPTK
                     DPAVIKVDEEAVQKWLKNGAQPSDTVRGLLKKANVLK"
     gene            43234..43464
                     /locus_tag="PLG49_01115"
     CDS             43234..43464
                     /locus_tag="PLG49_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOQ15940.1"
                     /translation="MRELVEVIAKALVDNPDAVQVNELEGERSVILELKVAPEDMGKV
                     IGKQGRIAKAIRTVVKAAAIRENKHVVVEILQ"
     gene            43554..44069
                     /gene="rimM"
                     /locus_tag="PLG49_01120"
     CDS             43554..44069
                     /gene="rimM"
                     /locus_tag="PLG49_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036250.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOQ15941.1"
                     /translation="MSNYMEIGKIVNTQGIRGDLRIIPTTDDPKRFELLDTVWVESKE
                     GTKEYEIEKVWYHKQFVILKLKGISSINEAEKLKNSIVKIPRELALPLGEGEYYIGDL
                     YDLEVYTNDEEYLGKIVDILFTAANDVYVVKKEDSKKELLIPAIKQCILEVDLNNRRM
                     TVNLLEGLREL"
     gene            44066..44797
                     /gene="trmD"
                     /locus_tag="PLG49_01125"
     CDS             44066..44797
                     /gene="trmD"
                     /locus_tag="PLG49_01125"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ15942.1"
                     /translation="MNFYILTLFPDMIESAMSHSITGRAQEAGIIKINCINIRDFANN
                     KHNQVDDYPYGGGPGMVLQPQPVYDAYQSIILKKNIKPRVIYMTPQGKIFSQAVAKDL
                     AKEKDLMIICGHYEGIDQRVIDEIVTDEISIGDYILTGGELAAMVVIDAVSRLIPGVL
                     GKEESYQDESFSNHLLEYPQYTRPLVFRNRKVPEVLLSGHHAKIEKWRREQSLKLTFE
                     KRPDLLEKAELTEEDKAYLDSLRKM"
     gene            44936..45283
                     /gene="rplS"
                     /locus_tag="PLG49_01130"
     CDS             44936..45283
                     /gene="rplS"
                     /locus_tag="PLG49_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOQ15943.1"
                     /translation="MNEIIKEIEKEQLKTDITPFEVGDTIRVYSKVKEGNRERLQMFE
                     GIVIKKQHGGIRETFTVRRLSYGVGVERTWPLHSPSIDRIEVVRKGKVRRAKLNYLRQ
                     RVGKAAKVKEIIE"
     gene            45342..45923
                     /gene="lepB"
                     /locus_tag="PLG49_01135"
     CDS             45342..45923
                     /gene="lepB"
                     /locus_tag="PLG49_01135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ15944.1"
                     /translation="MENKDNDSAESKEKTEKINKNFKELLEFIRDLIIIFVLAMFLRT
                     FVFENTRVLGPSMEPTLHDGDLIIVNKLEYYFTDPERGEIIVFPYKGDPSKQYIKRII
                     ALPGEVIDIREGKVYINGELYEEEYILEEMQLRGDIDFPYAIPADTYFVMGDNRNNSS
                     DSRYQDVGTIEKDKIIGHAVFRLWPINKFGQIK"
     gene            45946..46497
                     /gene="lepB"
                     /locus_tag="PLG49_01140"
     CDS             45946..46497
                     /gene="lepB"
                     /locus_tag="PLG49_01140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ15945.1"
                     /translation="MNEAVNKKIIKELFEWLRDIIIAALVTLFIINFLFQNTQVIGDS
                     MEPSLEDGHSVIINKFIYRFKAPQRGDIIAFKSPVNPSEHHIKRIIGIPGDTVEIKEG
                     KVYLNGILLKEDYILEPMDAKMAGDMEYPLMIGPDSYFVMGDNRNISYDSRYKEIGLI
                     DESSISGKALIRIWPLNKIGRVK"
     gene            46516..47049
                     /gene="lepB"
                     /locus_tag="PLG49_01145"
     CDS             46516..47049
                     /gene="lepB"
                     /locus_tag="PLG49_01145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ15946.1"
                     /translation="MGQKNKSFIFNEAMSWLRDIIIAVIIALFVRQFAFAHTVIPTGS
                     MIPTIQIDDHIFVSKLSYYFSDPQRGDIVVFHHDVDLIKRVIALPGEEIDLRDGKVYI
                     NGEPLEEDYLNEPMDTGITGFLEFPYKVPKDCYFVMGDNRNYSKDSRYFGPVHRDQIF
                     AKAGFRIWPLKSFGFVK"
     gene            47074..47931
                     /gene="ylqF"
                     /locus_tag="PLG49_01150"
     CDS             47074..47931
                     /gene="ylqF"
                     /locus_tag="PLG49_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOQ15947.1"
                     /translation="MNIQWYPGHMSKTRKIILENLKLVDLVIELLDARIPYSSKNPDI
                     DVLAANKNRIIVMNKSDLADSNITSLWIQHFKNYGYHAISVNSMDGKGISQVENLARE
                     LLKEKIEKDKKRGRIFRPIRAMVVGIPNVGKSTFINKLVGKASAKTGDRPGVTKGKQW
                     IKIKKDFELLDTPGILWPKFEDQTVGLHLAFIGSIKQEILDSHNLSLELLALLAKKFP
                     DCLSERYKITDIDSKSPIELLTEIGQKRGYLIRGGEVDYSRTSLMLLDELRSGALGHI
                     SLESPEEFY"
     gene            47977..48747
                     /locus_tag="PLG49_01155"
     CDS             47977..48747
                     /locus_tag="PLG49_01155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ15948.1"
                     /translation="MEKFSLQSIEKQLKEVSYDELPNFLEEIKKDSRIGVQKLVQKYE
                     RKYETYQVELKRIQEMSQYEKYYYQKGVKYIAGIDEAGRGPLAGPVVAAAVILPKDCF
                     IMGINDSKKLSLIKREELFEIINKKALSIGIGIVDPQTIDRINILQATFEAMKKALDA
                     LSLKPELVLVDGNQNIPDISVPQEDIIKGDQKSISVAAAAIVAKVTRDRIMDKFHELY
                     SEYGFNKHKGYGTKEHIEAIKKYGLCPIHRESFTRNLY"
     gene            48757..50382
                     /locus_tag="PLG49_01160"
     CDS             48757..50382
                     /locus_tag="PLG49_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15949.1"
                     /translation="MKINRYQQLASNSQKVYAANEKSLLRLKAGDIFKGQIIDIKQGK
                     LSLLLETGATVEAKLQSNVYELKIGQSLFFRVQSLTNEQIFIEILPDQWSDSKSNIIL
                     EALEAANLLPTDENYKMVEKLLEKQLPVDKDNLHAINQFIKSNKNVTLDKIIFLMDKQ
                     IPVNEKNISQLDGYIEQHIHLHKQIENLLDKIIKLSHSPIAEKILDCFLETGDNALTD
                     KDINPEEIINKADLLDKEAQRVRAGETELKNQLTEIRKEVELLTFMKDNQHIIETFMN
                     QHEFFTEEELFSFLMSEFPTSEIKALMSNDNHFFMFFAENMKYLLENKTDNQYLEKVL
                     NNKIRTFLNVLKEELYLPLEKLKNKEELKERFNKLYKYMNRLENLLTGQDSAQVKDLY
                     KTCQEIKNNLEFMNQFSKFDAFIQIPIKLNHHHSEAQLYVFRKKKTAKKSSHSLSVLI
                     ALDLAYMGHTEVFVQKNDKDLYLQFKLENDTYIKIMKEYIHRLLDVLSYKSYKIRSLN
                     FHKLEDSFNLLKTEENDYNINEEPRRFSFDMRV"
     gene            50404..50688
                     /locus_tag="PLG49_01165"
     CDS             50404..50688
                     /locus_tag="PLG49_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOQ15950.1"
                     /translation="MENNKRIKKAAALHYEKGSTAPKVIAKGKGVIAEKIIEKGLEEE
                     IPVYEDEKLANVLTELEIGELIPVELYEIVAEILVFVGDIDRLQEKIKNG"
     gene            50681..51040
                     /locus_tag="PLG49_01170"
     CDS             50681..51040
                     /locus_tag="PLG49_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOQ15951.1"
                     /translation="MVNKRLIGDWGEDIASDFLEKEGIKIIEKNFRCKLGEIDIIAEE
                     DDYLLFIEVKYRKDLSHGYPREAVTFHKQRTIAKVALWYLKKYNLWHRSCRFDVLEIL
                     GEKPDLKICLIRNAFVV"
     gene            51073..51900
                     /gene="pgeF"
                     /locus_tag="PLG49_01175"
     CDS             51073..51900
                     /gene="pgeF"
                     /locus_tag="PLG49_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOQ15952.1"
                     /translation="MYHNLSNQLSFHNKNDLYYITFPAFDASQQVRHCFSTRLGGLSQ
                     GPYQSLNLGLGRGDDDEKVYGNYKLLCEAIGINHEKLIFTNQIHDNHIKIIRKEDLEK
                     YNMKNWVKGTDGLVTNIPNVPLVTLYADCVPLFFLDTKKKVIGLAHAGWRGTVKKIAL
                     RMIEIFINEFNSNPKDILAAIGPSIGKCCFEVDFSVADAFQKAFKNTHKIIFPIENEK
                     FMIDLWEANKEALTEGGLPLSNITVTDLCTKCHHDIFFSYRADKGQTGRLAAVMELI"
     gene            51961..53298
                     /locus_tag="PLG49_01180"
     CDS             51961..53298
                     /locus_tag="PLG49_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOQ15953.1"
                     /translation="MKNYKEHKIKEVLPGSIAEELGIEAGDLLIAINGQEIEDVFDYR
                     YLISDEFVTLTICKKDMEWWEYEIEKDVHEDIGLVFENALMDHLKSCRNKCVFCFIDQ
                     LPPSMRKSVYFKDDDWRMSFLNGNYITLTNMKEKDFERLIYYHLSPVNISVHATDPEV
                     RIKMLNNRFAGDVLGKIKRIVDAGIEVNCQIVLCKGLNDGLVLKKTLDDLEKFFPGIK
                     SISIVPVGISKYRENLYPLIPFEKDDAKEIIRMVHHLQDFYKEKYGSYILYCADEFYI
                     TAGEEIPPVHRYEDFPQIENGVGMLALMEEEFNAAFLDLPDEISGKIDLSVATGIAAF
                     PFIKKLVNKLLDKYSNLTIRVYPIKNDFFGDKITVSGLMTGQDIIKQLNGKELGSKLL
                     LPENAFRNNDTIFLDDVYLDDLEKEFRIPVEIVGVSGKDFIHAILNAKENRDE"
     gene            53291..54616
                     /gene="der"
                     /locus_tag="PLG49_01185"
     CDS             53291..54616
                     /gene="der"
                     /locus_tag="PLG49_01185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOQ15954.1"
                     /translation="MSKPIVAIVGRPNVGKSTLFNRLAGERIAIVEDTPGITRDRIYA
                     DTEWLNHKFTIIDTGGIEPDSDDIILKQMKRQAEMAIEMADVIIFLTDAKQGLTDADH
                     EVANMLRKSKKPVVLAVNKVDHWGKDQMEVYEFYNLGLGDPIPISASQPLGLGDMLDE
                     VVKYFPQQEVSDYDEDVIKVAIIGKPNVGKSSLINKILGEERVIVSDIPGTTRDAVDV
                     PVTIGEDKFVFIDTAGLRRKNKIKENIERYSIFRTVAAIEKADVAVLLIDAEAGVTEQ
                     DTKIAGIAHERGKASIIAVNKWDKIEKDDKTMNKYLKDIEVKFSYMSYAPKVFISALT
                     GQRINKLFEMIKVVSQNHALRIETGVLNELLYEAMAMNEPPSDKGKRLKIYYMTQVSV
                     KPPTFVLFVNDKELMHFSYERYIENQIREAFGFKGTPLHFIIREKQGRE"
     gene            54621..55229
                     /gene="plsY"
                     /locus_tag="PLG49_01190"
     CDS             54621..55229
                     /gene="plsY"
                     /locus_tag="PLG49_01190"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOQ15955.1"
                     /translation="MFRLVCIIIGYFLGCFQTAYIIGKKVQKIDIRNYGSGNAGTTNV
                     IRVMGWKVGIITFIGDFLKAVIAVFICKLIFPDCPEAGLYAGLGTIIGHNWPVFLKFK
                     GGKGIAATMGTLLAFDYRLGLICITIMALVLIISRIVSLGSIIMSISIPICLYLFYND
                     LEITFLGVLFTISAILRHRDNIRRLFKGQETELGQRTNEKYQ"
     gene            55253..56293
                     /locus_tag="PLG49_01195"
     CDS             55253..56293
                     /locus_tag="PLG49_01195"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOQ15956.1"
                     /translation="MEKIAVMGAGSWGTALAILLAKKGHHVTLWCYLESEKEQLESVR
                     ENLNYLPGVPIPQNIIITNDYKEAIVNNEIIVIAIPSKFLRSNMEQFSPYLTPRHLVV
                     NVSKGLENHTLLRMSQVIEETAPQCPVAVLMGPSHAEEVARDIPTACVSSSRSQKIAE
                     RVQDLFMTPTFRVYTNPDLIGVELGGAIKNVIALAAGVVDGLGYGDNTKAALMTRGMV
                     EITRLGLAMGADIQTFNGLTGIGDLIVTCTSMHSRNRRAGILIGKGKSLDEALNEVQM
                     VVEGVHSAKAVYELSRKYNVEMPIIREINQVLFEGKSPREAVMDLMMREKRPEHQTEE
                     IHLAHKVSWKDE"
     gene            56422..>57665
                     /gene="spoIVA"
                     /locus_tag="PLG49_01200"
     CDS             56422..>57665
                     /gene="spoIVA"
                     /locus_tag="PLG49_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOQ15957.1"
                     /translation="MDNFNIYQDIADRTNGDIYIGVVGPVRTGKSTFIKRFMDLLVIP
                     NIENSYNKERAKDELPQSAAGKTIMTTEPKFVPNESVSISLNDEIELNVRMIDCVGYM
                     VEGATGHLEGESPRMVNTPWFDYEIPFTEAAEIGTRKVISEHSTIGIVVTTDGTITDI
                     PRKDYEEAEERVIRELKELNKPFVVLLNSAKPYDAQTQELNEELSQRYDVPVITCNCA
                     QLKMDDINRIMEKVLYEFPVQEIQFMLPKWVETLDRDHWMKKRIIEAVKDTIYPVYSF
                     RDLKDAISSFEDYDFVKKVFQEKINLGKGLVKIDITVSDELFYRVLSESTGMEIEGEH
                     QLISLIVKLAAIKKEYDKVADALKEVRQKGYGVVMPLLDELKLEEPEIVKQGNRFGVR
                     LRASAPSIHLIRADIETEVSPIV"
BASE COUNT        20679 a         7894 c        11705 g        17387 t
ORIGIN      
        1 aaaacgggag atttaatttt aaaagtcaat gaaaaaagta tagaaaataa agaagaactg
       61 atttctgcta ttgagaattc caatggaaaa gtcttaaaaa tgaccataaa aagagataat
      121 gagctgcttg aaatttatgt taccccgata aaaagtgaaa tcgataaaag ctataaaata
      181 ggagcctggg tacgggatga aacccagggc attggaacga ttacttatta taatcctaaa
      241 agctatacct ttggtgcttt aggtcatggt ataacggata tagataccag gaagattatt
      301 cctataaaga acggaaaaat catgaaagca gaaattactt ccataaaaaa aggtcgaaga
      361 ggaatacctg gagaactgac cggagctatc tatgacacag aagaagctca gttagggacc
      421 attgaattaa ataccagtca aggtattttt ggaaaattag accctaaaaa aatgaaagct
      481 tttcctaaaa aaagttttcc tattgctttt caaaatgaaa tacatgaggg aaaggcaatc
      541 attctatcga atatttcggg tgacaaaata gaagaatttg aaattgtaat acaaaaagta
      601 tccagatttc aacaggatat ttcgaaagga atgattatta aaatcactga tccaagacta
      661 ttggataaga cgaatggtat tatccaggga atgagtggca gtcctatcat acaagatgaa
      721 aaactggtag gtgcagtaac ccatgttttt gttcatgatc caacgaaagg ttacggaata
      781 tttatagaaa atatgctaaa aaatgaaagt gatttggaat aaaattgaaa tatatactct
      841 attttattaa gaagttcttt tttgtgaata atcaatcttg attttatttt atgcattcat
      901 tataatagga atagattgga tatttctttg gaaagtgagt ttttcaaggc ttatgctaag
      961 ataagccgtt atcaaattac aagggggtca tttttttgaa tacggataaa attaaaatcg
     1021 ttttagcaga tgataacaaa gatttttgcg aaattttagc tgaatacctc aatagacaaa
     1081 gtgatatgga agttattggt acagctaata atgggacaga agcatatgag atgattttag
     1141 aaaagactcc ggatgttgta atattggatg tcattatgcc ccatttagac ggaataggtg
     1201 taattgaaaa gcttaaaaaa gttcaaatgg aaaaaagacc agtatttgtg atgctttctg
     1261 ctgtaggaca agataaaatt actgaaaaag cattaaattt aggggcggaa tattatatta
     1321 taaaaccttt tgatatggaa acccttatta atcgtattcg tcagttaaaa ggacatcatg
     1381 aaattataaa aagcagaggt tcaacgatgt cccttagcaa aggttcttat gtacccactg
     1441 ctcataattt agaaacggaa gtgaccaata ttattcatga aataggtata cctgctcata
     1501 ttaaaggata tcagtatctc agggatgcaa ttatcatgtc tattaatgat atggagatat
     1561 taaattctat tacaaaacaa ttgtatccat ccattgctaa acaatataat acgacgccca
     1621 gcagggtaga aagagccatt cgccatgcca ttgaagtagc ctggagcaga ggaaaaatgg
     1681 ataccattaa taagcttttt ggttatacaa ttaataatgg caaagggaag cctacgaaca
     1741 gtgaatttat agctcttatt gcagaccgtt taagattaga gttacaagtg ggctaatatt
     1801 ttataaaaag ctttcttact tattcgaagt aaggaagctt ttttatttgt tatatttatt
     1861 gaaaaatcat ccatactaat tatagataag gtagtttgag atgttcagtg tagggagata
     1921 atgagcagga ttgatcataa aaggaaaagg cttgtttgtg ctgaaaggag tgaaaaattg
     1981 agtatagtag gcgttgccaa aattgacagc agtactaaaa aactcatcca ccggctgact
     2041 cctaaagata ttgcgattat cgatcatgaa gatttagatt atgtatgtgc ctatgcatta
     2101 gcttctaaaa aagttaaagc agtcatcaat aaatcctctt ttagttcagg aaaatatcct
     2161 aatcgggggc cttatatcct tttagagcat caaattccct tatatgaaaa tatagaccat
     2221 tcgattttta agtatattaa agaaaatgac aaaattgaaa taaaagataa tttattatat
     2281 aaaaatgaaa ttgccgttcc tttaaaatct attagtaaac gggattgtga ccaatttcaa
     2341 aaaaaatcct tgaacaatct taaaaatgaa atgtttgctt ttattgataa caccttatat
     2401 tatatgaaaa aagaaaaagg taaattattc catcctccca gactccctgc tatcaatatt
     2461 cctgttgccc accggccagt attgcttgta accaggggag aatattatat tgaagactta
     2521 atgagactag aaaattttat taaaaaacag cagccagttt tgctgggtgt tgatggtgga
     2581 gcagacggac tggcatttct aaattatatt cctgatatta tttttggtga tatggacagt
     2641 gtatcggatg aaagtttaaa aaaagccaaa aatattgtat tacatagtta cgacaatggt
     2701 atctgtcctg gagaggcgcg tataaaaaaa cttggtctgc cttattctaa atactcttgc
     2761 tttggtacca gtgaagatgg ggcaatttta atgtgttatt atatgaacgc ttcttatatt
     2821 gtcattgtag gaagtcataa taacttaatc gactttttgg aaaaaaaccg aaaaggtatg
     2881 gcgagtactg ttttaaccag aatcttagta ggttcgaaac tcattgatgc aaaaggattt
     2941 tttaagcttt tttaaagatg gtgattattt ttgaatgaaa agatatccat aattattccg
     3001 gtttacaatg aagaagactt tataaaggat acattaaaag gacttttaga agcggatata
     3061 aaaaacttag gggaaattat tgttgtggac gatggttccg tagacgaaac gaaaaagaaa
     3121 gtctgtgaaa tcaaaagcgc ttttatttat tattattctt tagcccgtaa cagggggaaa
     3181 ggggctgcct taagaaaagg tctggaactt tccagatacc cggtgattgt ttttttagat
     3241 ggggatttag gctattcatc taaggaaatt aaaaaacttt taaaagcgat tgatgataag
     3301 agtgtagatt tcgtgattgc tgattttcct ccccctttgc ataagggagg ttttggtttt
     3361 gtaaagaaac tttcgcaaaa aggcgtttat tttttgacag gtcaaaagat aaaaaatcct
     3421 atatgcggac aacggatttt tagaaaagag attcttgcag atattgcaat tcctgatggt
     3481 tttgcagtgg aagtgggcat gcttattgat cttttaaatc atggtttccg agtgcgggaa
     3541 attcctgttt ttatgaggca cagagaaagt aagagagatt taaaaggttt tattcataga
     3601 gggaaagaat ttattcaaat tttgcagata ttaataaaaa aatattttca gtataagaat
     3661 ccctggaatc gatggtgaag ctatgatttt ttatttgatt ggaggactta taggtttttt
     3721 gagctctttt ttttggagag atattatatt agatttttta ataaaatata attttaaaaa
     3781 agttaattac agaaataaaa aagttcctgt aggaatgggt atcttattat ttttttcaac
     3841 cctgattgct gcttatttta ttattttttt tactgagcag gcttttatct attatctttt
     3901 tttatttggt ctttcgctca ttgcatttgc tggtattctg gatgacagta ttgatgaaaa
     3961 agaggtaaaa ggattaaaag gacacttaag gaaattattt aaaggagagt tatcagctgg
     4021 tgcaataaag gcattagcgg gaatttttgc agctttttat atagcctttt tattatcagg
     4081 gactttaagc gatttaattc ttaacttatt gttgattctt ttttctataa atatcatcaa
     4141 tttatttgat ttaagaccag gaagggcttt aaaggtattt ttcttaatga gtgctataat
     4201 ttggctgatt ggggattttt ctgacaagta tttgactttt ttactgatag gcagtagttt
     4261 tcctatatgg aagggggatt tgaaagaaca gtacatgttg ggggatatag gcgctaatat
     4321 attgggctat agtctgggtt ttactgccgt gatttcctta acgccttatt ataaaatgct
     4381 tagtattgga ttatttcttt ttcttcattt aattgcagaa aaaaaatccc tttctgaaat
     4441 cattcttaaa gttccttttc tacactattt agataatctt ggaagggata aaatacaata
     4501 aggctggttt ttaccagcct ttattgtatt gactttgaaa tcaattgttt aatttttatt
     4561 tcatcggggg tgatataaag ggttttttga ttttcataga tgaccattcc aggcttagca
     4621 cctctgggtt ttttaacgtt ttttcttaag gtatagtcta caggaacatt actggagttt
     4681 cttgctttac tataatatgc ggcaagatgg gcagccagaa ggagggtttc ctctggtact
     4741 tctttatttt ctgtttttat aatcacgtgg gaaccaggaa tgtcttttgt gtggagccat
     4801 aaatccgtag gagcagaatg cagagttaat tcatcatttt gacggttgtt ttttccaacg
     4861 taaatatcaa agccttcggc agatttaaaa tgcaggggtt ttgattttac tttttcatta
     4921 gaactttttc cttttttatt tttaatatag ccttcttctt gtaattcttt tttgatatcc
     4981 tcaatatctt caatatctgt agaagcttct aaggaagaaa gcaaggattc cagatagctg
     5041 atttcttcat ttacccgttt aagctgctct tggacggcat ttttcgtcct ttttgcctta
     5101 ttgtatttat taaaataata ctgggcattt tgagctggtg ttaaaacagg gtctaaggga
     5161 atggttagtt ctgtcatatt ttcatcataa aaatttacgg tagtgaatga ggtcattcct
     5221 tttgaaatag agtatatatt agaagtaatc agttctccaa aaacccttaa ataatcacag
     5281 tctgatactt cctgtaactg gcggatctga atctcttttt tcttatagca tctttccagg
     5341 ttgttttgaa tgattttatg aatgttcctt gacttttctt tgattctgga aagcctgtct
     5401 tttaccttat aataatcttc aatggcttct gaaacagagt cataatattt ttttgcatag
     5461 cctgtaaatt ggcttaaatc aatagaagaa aactcaacag gctcaccagt atggggatca
     5521 taaataattt gaggctgaaa aagattagct tttacttgtt taaaaatccc attcatgact
     5581 ttagatagcc gttttaaatc ttcgtctctt aattcttcta agtaagcaga aacatcgata
     5641 ttagccctgt gacatatttc tgaagcaaca agaggactga tgccagtaaa agttttgtaa
     5701 atggtttgtt gcagttttgt acctttattg cctttaagct gtgttacaaa gtcagcttct
     5761 tcaaggctta aaggatttat tttatcctga gatggtggcc tttgatagat ttttcctggt
     5821 aatacttctc tgacagaact tttatccttt gaaacccgga tcacactgtc taagatacag
     5881 tttttttcgt ccgttaagat aatattgcta tggcgtccca tgatttctat aatgagcttt
     5941 ttaaccgtta agtcacctaa ttcattaatg ttttcaatat gaaattctat gattctttca
     6001 aaatctggct gaacaacatc gattaatttg cctccgctta aatgctttcg aagaagcata
     6061 caaaacatcg gaggacttaa gggatttttt ctttgaatat ttgtaatgtg gcttctgggg
     6121 taattgctgt tagaattcag caataagcgg taattggccc cgtttcctct tatttgaaga
     6181 ataatttcat ccgattccgg ttgataaatt ttatcgattt tgccagccag cataagtttt
     6241 ttgatttcgt aaacaatatt agaaattaca aggccgtcta atgccataaa aattcctcct
     6301 tttagtctta gattagtata ccattaagtc ttttgcttta aaagtattta tctttacgat
     6361 ttaaaaggaa gtatactgtc cctgtctctt ttattgactt agccaaaaag ctgcgatata
     6421 atacaaaaat aagagaaatt caaaggaaaa aaagggaaga gtgaataaaa tgattcgtag
     6481 catgactgga tacggtcagg ggaagcttat ttcggatgac agaaagttta cggttgaaat
     6541 tcgttctgtt aatcatagat ataatgatat taatgtacgg cttccaagga ctttaaatta
     6601 tcttgaagac aaaataagaa cctttataaa agaaagggtt tcaaggggta aaatagatgt
     6661 ctttgtttct tttgaaacga cttcaaaaga tgattttgag gtttacttaa atgaaaactt
     6721 attggatgcc tatttagaac aacttaatat aattaaaaat aaaagcactg tgattgatga
     6781 tatatcggta tctttattag caagatttcc tgaggtcatt atcgttaata aaaaagaaga
     6841 cgataaagat attttatggc agttgttaga ggaagcttta aaagaagctt ttaatgattt
     6901 tcttgccatg agagaaaaag aaggagaaaa tttaaaagag gatttattgg ctaaattaaa
     6961 agtttgtgaa aattatttgg ctaaaattaa ggaaagaagc ccaaatctgg cagaggaata
     7021 taaaaataaa ttagaaaaaa gattgcagga gcttttaccg gatcattcca tcgatgagaa
     7081 ccggcttgct atggagatag caatttttgc tgaccactgt agtatcgacg aagaaattgt
     7141 ccgtctagaa agtcatatta atcaatttag aaatatctta aatcatgaag aaatcgttgg
     7201 acgcaaatta gattttttag tacaggaaat gaaccgggaa gtcaatacca taggctctaa
     7261 agccaatgac cttcaaatta gtcagagtgt gatagaatta aaaagtgaaa tagaaaaaat
     7321 tagggaacaa attcaaaatc tcgagtaaag attgtttagg aggttaatat gggtataaaa
     7381 ttaattaaca tcggatttgg gaacattgtt tctgctaatc gaatcatctc catagtaagc
     7441 ccggaatctg cccccattaa gagaatcatt caggaagcaa gagaccgtgg aatgttaatc
     7501 gatgcgactt acggcagaag aacaagggcc gtgattatta tggatagtga tcatgtgatt
     7561 ttatctgccg tacagccaga gactgttgcc aaccggctga cttctaaaaa tcaggatagt
     7621 cttaatactg aaatagatga ataatatagg tgataaatca tgaataatac taaaggaatc
     7681 atcgtagttg tctcgggccc ttctggttca gggaaaggca ccatcgtcag tgaattaata
     7741 aaaaatgagc agtatgccct ttctatttca gccacgacac gaaaacccag attaggagaa
     7801 gaagaaggga ttcactattt ctttaaaagc aaagaagaat ttatggatat gattgaaaag
     7861 agagaattag ttgaatgggc tgagttttgt gataattatt atggtacacc caaattttat
     7921 gtagaaagta agatgaacga aggtaaagat gttatattag aaatagaagt ccagggggca
     7981 ttgcaggtta aaaaacttta tcccgaagct gttttgattt ttattgttcc tccgagttta
     8041 aaagaactaa agaacagact gattaatcgg ggaacggaaa gcagtgaaat cattgaaaaa
     8101 aggcttagac gtgccctgga agaattagaa tttataaatg actacgatta tctggtcatc
     8161 aatgactgca ttgaacaggc tgtcatggat atcaatacga ttattaaggc tgaacattta
     8221 aaggtgaata aaaggatgtc tttaattgaa aaatttaaag aggagtgatg attgtgctta
     8281 aaccttcata ttcagattta atagaaaaat taaatgaaaa aagttctgat gaggaaaaac
     8341 cgattattac gagccgctat tccattgtta tagcgactgc caaaagagcc cgcgaaattg
     8401 tagataaagg ggatccaagg gtagaatcta aatctaataa acccgtatcg gttgctgtag
     8461 aagagcttta ccagggaaag accattatta aatataatga aagtaaaaca ggccataatg
     8521 ttgaagaaat agaagaagta tttgaggaag atgtgaaggc tagtgacaat gaagataatt
     8581 aatcagagat aattttttgc aatcgccctt tgggcgattt tattatacta gagcaaagaa
     8641 ccatatataa atactatttg ctaaagaagg gaagaaatta aatgatgaat aaaaccgttg
     8701 ttctgggagt aacaggaggt atagctgctt ataaagcctg tgaaattgcc agccgcctgg
     8761 ttaagtcagg aatagaagtg aatgtgatta tgaccaggtc tgcacaagaa tttgtaagac
     8821 ctttagtttt tcagtccctt acgaacaggc ctgtagcaac agatatgttt aaggaacctg
     8881 ttacctggga tattgagcat atttctcttg ctaaaaaagc tgacttgttt ttaattgcac
     8941 ctgcaacggc aaatattatc ggaaaaattg cggcagggat tgcggatgat atgctttcaa
     9001 caacggtaat ggcaacaaaa gctccggtag tcattgctcc agcaatgaat actgccatgt
     9061 ataataatgt tattgtacaa agaaatatac aaactctatc ggatttagga tatcatttta
     9121 taagcccggc agaaggccgt ttggcttgcg gggatacagg tataggaaaa ctggctgatg
     9181 tagatattat tgttgaaaaa gtcctggagc ttttagacca gcaaccccaa gattataaag
     9241 gtaaaaaagt cgttgttacg gcaggaccta ccagagaagc tttggatcct gtccgataca
     9301 ttagtaatca tagttctggg aaaatgggct atagtcttgc agaagctgct gtaaaaagag
     9361 gggcggaagt tattttgatt tcaggtccta ctcatattaa gcctccttgt ggttgtcaaa
     9421 ccatttttgt aacaacgaca gaagaaatgt atcaggaagt cataaagcat tatgagaatg
     9481 tcgatgttct gattaaggct gcggcgccca gtgattacac tcctgaattt gtatcagacc
     9541 ataaaataaa aaagaacaaa gaaggactca ccataaagtt aaaattaaat aaagatatac
     9601 taaaagaatt aggcagatta aaaaagaagc aaattcttgt tggttttgca gctgaaactg
     9661 ataatgaact ggaatatgga aaaaagaaat tagaagaaaa aaatttagac atgatttgta
     9721 ttaataatgt tgcacaggaa aatgtcggct ttggctatga taccaataaa ataactcttg
     9781 ttaagaaaac aggagaagaa aaaagtctgc ctttaatgag caaaaaagag ttagccaata
     9841 tgattttaga tgaaataaaa gacttgttga tggagcagga ccatatttaa agggtggaaa
     9901 aaatgattgc agaagtaatt gtaggcatat ctcatgaaaa actggataag atttttcatt
     9961 atattgtacc gaaagaatta gaatcaaaag ctcaaatagg ggtgagggtt cttgtacctt
    10021 ttggaaagag caacaaaaaa atagaaggct acattattgg tttttgtgaa aacagttcta
    10081 tagaaacaga taaattaaaa gaactttatt ccattgtaga taagagtcct ttgtttaatg
    10141 atcatatgct tgctttggcc aaatggatgc gggaatatta tggtgctaat atggtagatt
    10201 gcctgcgctg tattctccct tctgggctaa aaataaaggc acagactttt gtcagactta
    10261 atccggattt taagaacccg gaagttttag agtcggaaaa acgaacagaa aaagaaaaag
    10321 aaatcctttt atatttattg aataaagaca gctatgttct tatggaagat ttattggatt
    10381 tatttggagg acaaattaaa aaagaactaa aaaagttaag agatgaaaaa ctgcttgaag
    10441 ttcgagagaa agagcttata aaagattata agtgtgttat aaaatacgta tctcttcata
    10501 aagaaatgac catggagcaa ttggaaaata tcctgatgga atatcagtcc aaaaaaaatt
    10561 acagggcgca atgtagaata atcgaatttt taatgatgaa tgagaccatc cctcttaatg
    10621 aaatgaaaaa aatattagaa gtatccagtc cttcaattaa tgccctgtta aaaaaagata
    10681 tcattgaaat tgaagaaata gaggtcagca gggattcata cagacactta tataatgaga
    10741 aaacagaagc ttttacacct acttttgagc aggcaaatgt gataagatat gtattaaata
    10801 aaatagatag cctaaaagga ggaaaaattc ttctccatgg tattacagga agtggaaaga
    10861 cagaagtcta tctccaactg attgaagaag tcataaaaag agagggtcag gcgattgtcc
    10921 tgattcctga aatatcttta accccacaaa cagttcagag atttaaaagc cgctttggtg
    10981 aaaaagtggc tgtgacccac agtaaacttt ctctggcaga aagatatgat cagtggaaaa
    11041 gggcaagaga tggggaagtt tctgttatga ttggtcccag atcggcagtt tttacgcctt
    11101 tttcttcttt aaaattaatt attattgatg aagagcatga aaacacctat aaatcagaat
    11161 ttgctcccaa atatcatgcc agagaagtag cagaaaaaag atgtgaattg actggagcgg
    11221 tatgcttaat tgcttcagca acgccttccc tggaagctta ttatggagca aaaacaggga
    11281 taagagactt attggaattg acttatagag cgaataatag tagccttcct caagtagaaa
    11341 ttgtcgacat gagaaatgag ttagaagaag gcaaccgctc aatttttagc aattatctta
    11401 aagaagagat gggaaaaaaa cttaagaata aagaacaaat tattttattt ttaaacagga
    11461 gaggcttttc taattttgtt tcttgcagaa aatgtggata tgtattaaaa tgcaaaagtt
    11521 gcagtatttc ttatacctat catgcctata accattcttt ggtttgtcat tattgcggtg
    11581 acaggatatt aagcccttct gtttgtcccc aatgtggctc aaagcacatt aggtattttg
    11641 gagtgggaac agaaagggta gaaaatgaag tcaaaaaatt atttcctgaa gcttcagttt
    11701 tgagaatgga tttggataca actaaaggaa aatatggata tgaaaaaatc attgagcaat
    11761 ttagaactca aaaggcagat attttaattg gtactcaaat ggtagcgaaa ggtcatgatt
    11821 ttcccaatgt gactttggta ggtattttag cagctgattt aacacttcat attcaggatt
    11881 ttcgttcgtc tgaaaggact tttcagcttc tgactcaggt aagtggaaga gcaggcagag
    11941 gtgatttacc aggaaaagtc attgtccaga cctatgatcc ggagcattat agtatcgtag
    12001 cggctaaaaa tcacgacttt ctgacttttt ataatcagga aatcagcatt agaaaagaac
    12061 tggattatcc accttttact catatcttta cggttttatt ggttgcttcc aatgaaaaag
    12121 aggtcatcca atctgcttac aaactgatgg agataatgaa atattataac aggaaaggga
    12181 attttaaatt atatggacct gtacctgcca tgatttctaa aataaaaaat aaatacaggt
    12241 ggcggatcat tattaaatgt aatgacagga atctattaat gcaatatgct ttttattgca
    12301 ttgaccagta taagaaaaag tttgataata accagctatt aattcaaacg gatattgacc
    12361 cgctgatgat ttattaagct aaaggaggaa aaaatatggc tttaagacaa atcagaaccg
    12421 acggagatga aatattgaga aaaaaggcaa aaaaagttga acaaattacg ccttctatta
    12481 ttacgttact ggaagatatg gtagaaacga tgtataatgc tgacggggtg ggtctggcag
    12541 cacctcaggt aggcgtttta agaagagttg ttgtgattga tataggcgag ggggttattg
    12601 aactcattaa ccctgaactc attgaaacag aaggagagca aatcggacca gaaggttgct
    12661 taagtgtgcc aggtgttaca ggagaagtgg aaagaccttt gaaagttaaa gtaaaagcct
    12721 taaacagaaa aggtgaagaa tttattatag aaggagaagg ccttcttgcc agggccttgt
    12781 gtcatgaaat cgatcacttg gatggcatca tttttaccga taaggtaatc cgctatttag
    12841 atgaggaaga agaataagaa aaggggtatg agaatgaaag tcgtttttat ggggacacca
    12901 gattttgctg ttccgacttt acaaaaatta attgatgaaa agtatgaaat tgctgctgtg
    12961 gttactcagc cagaccgtcc caggggcagg gggaaaaaaa ccctggctcc tcctgtaaaa
    13021 gaattggctc ttcaatatca tattcctgtt tatcagccag agaaagttaa aacacctgaa
    13081 tttatagaca gccttcagaa aattcatccg gatttgattg tggtggtggc ctttggtcag
    13141 atacttccta aggctatatt ggagatgccc caatatggct gtattaatgt tcatgcttct
    13201 cttttaccta aataccgggg agctgctcct atccaatggt ccatcattaa tggagaagaa
    13261 atcacaggtg taaccaccat gtttatggat gaaggaatgg atacaggaga tattttatta
    13321 aagaaagaag ttaaaatata tcctgaagat acggcccagg aacttcacga cagactctct
    13381 gttgaggggg ccagcctttt aatagaaaca ttaaaacaac tgcaaacagg gcaattaatt
    13441 aggcaaaaac aaaatgacca ggaggcaact tatgcaccga tgttgcaaaa agaaatgggc
    13501 tgcatcgatt ggtcaaaatc ctcaaaagaa attattaatt taattagggg tttatctccc
    13561 tggccaagtg cctatactta ctataaagat caaatgataa aagtttggaa agctgaagaa
    13621 tatcatgaga aatatgatgc tcatgtcatg ggagaaataa ctgaaatcat aaaagataaa
    13681 ggtattgttg taaaaacagg agatggaagc cttatgataa cagaaattca ggctgaaaat
    13741 aagaaaagaa tgtctgttgt tgattattta agaggacata agatagaaaa aaatattgtt
    13801 ttaggcaata atagaaatga aataaaataa gcaggggtga tagaatgtat attgatacct
    13861 attatatcat ttttgtatta ccagcaattc ttttaaccat ttatgctcag gcaagagtac
    13921 attcaacttt taacaagtat ttaaaggtta gaaatttaag aggtatgact ggggcagagg
    13981 cggcaagaat catactgcaa aaccagggga tatacgatgt aaccatcgaa agaattcctg
    14041 gaaatttaac tgatcattat gatccccgaa cgaaaaccct tcgactgtct ggtccggtat
    14101 atgatggtac aagtatcgca tcggtagggg ttgctgccca tgaagcgggt catgccatac
    14161 agcataatga aagctatagt ttcttaaaag taagacattc tatttttcca gttgtcagta
    14221 ttagttcaag aatggccatt cctttgatta tgcttggatt tgttttgtcg ttttctttcc
    14281 ttgttgaatt agggataatc ttattttgtg cggtggtatt ttttcaaatc attactttgc
    14341 cagtagaatt taatgcgtcc aaaagagctt tagttgcttt agaaggacag gggatattgt
    14401 cggacaatga aataagacct gcaaggaaag ttttgagcgc tgctgccctg acttatgtcg
    14461 cagcggctgc agttgcagta gcaaatttgc tccgcttaat attacttgct aatggtggtc
    14521 gcagaagaag gtagcagaaa agtcatctat aaaaagcgtt actatgtaac gctttttatc
    14581 atgaatgata tataaaaatt aaggagaata ttagattcta ttgaaatgta atttattgat
    14641 taaggagtac gcatatggaa aaattaaagc caagggaaat agcagtcaat attatttctg
    14701 aaatcaatga agaaggtgca tatagcaata tatctcttaa ggataattta agttattacg
    14761 gttacttaac ggatttagac aaagccttta ttacaaatat tgttaatgga acagtccgct
    14821 atcttttaac cattgactat gtcattaatt ctttttccaa aataaaaaca aaaaagatga
    14881 aagcaattat tagaagtatt ttaagagctt cggtatacca gatattgttt atggataaag
    14941 tacctccttc agcagcttgt aatgaagccg ttgaaataac aaagaagaaa ggtttatccg
    15001 gcttatcggc ttttgtaaat ggagtattaa gaaatatcgt acggcataag gatatgattt
    15061 cttatccgga tccaaagaag gattttctga attatttgtc agttagatac tcttttcctc
    15121 aatggatttt agactactgg ctgcaatatt atgaagcaga tttcgtagaa gaattatgtc
    15181 aggcatcgaa tatcaatccc ccgatcagta ttcgttgtaa tttattaaaa aatgatattt
    15241 cctctttagc aaaatcttta ttaaaagaag gagttcaagt ttcccaaggt ttgtatttaa
    15301 aagaagctct ttatatttct aagacttcat cacttggaga cttagaatcc tttaataagg
    15361 gtttatttca agttcaagat gaaagttcca tgctggtagg acatattttg gctccagaga
    15421 aaggggaaag aattcttgac gtttgcagtg ctccaggagg gaaagcaacc catttaggag
    15481 aattgatgca taacgaaggt tatatattgg ctagggatat tcatgaaaaa aaattaaatt
    15541 tagtggcaga cactgctaaa agattaggga tttgtatcat tgaaaccgaa gaaaaagatg
    15601 caactgaact ggatgaaaag ctggtaaata caatggataa ggttttagtc gatgcccctt
    15661 gttctggtct tggaatcatc cgtaaaaagc cagatatcaa atggaaaaaa gaaaacaggg
    15721 atttagagag cctgataaat attcaaagaa agatattaga aatttcttcg caatatctga
    15781 agaggaatgg ggttctggta tacagcactt gcactatctc tgaaaaagaa aatcttgaaa
    15841 atataaaatg gtttattaaa aattttgatt ttgagctcga agaccttaat ccttatattc
    15901 ctgaaagtat tcaaaatcat gatacaaaaa aaggatatat acaattatac cctcatattc
    15961 ataataccga tggtttcttt attgcaagac tgagaaaaaa ataggaggat aaaatgcaag
    16021 atagagaaat tgcttcatta accgtagaag aattgaagaa gcttatgctg gaaatgggag
    16081 aaaaagaatt ccgtggagag caaatatttc attggattca tgggaaaagg acagtagctt
    16141 ttgatgaaat gacaaatctt ccaggagaat taagggaaaa gcttaaagca agttttgatt
    16201 gcaatcttgt tagcctggaa caaaaatata tctctaaaat tgacaaaaca ataaaatact
    16261 tgtttcgtct aaaggataat aatataatag aaagtgtttt gatgagttat aattatggaa
    16321 atagtgcttg tatttcatct caagtgggtt gcagaatggg atgtgctttt tgcgcatcaa
    16381 ctttagaagg gttgatacgt aatttgtctg ctggtgaaat ggcaggacaa atttatcaaa
    16441 ttgaaaaaga tagcaataga aaaatatcaa atattgttat tatgggtgct ggagaaccct
    16501 tagataatta tgataatatt cttaagttta tctcgatcat caatcatcca aaaggtgcaa
    16561 atattggtca aagacatatt accttatcaa cctgtggttt agtggataaa atatatgatt
    16621 tggcagataa aaatttacaa attactttag cgatttcttt gcatgcgcct aacgatagga
    16681 taagacagga aattatgcct atagctaaaa gatattatat ggatgaatta ttaaaagctt
    16741 gttcttatta tattgaaaag acccatcgaa gaattacttt tgaatatgct ttaattaatc
    16801 aggtaaatga cagggaagaa catgcttacg agctggcaga taaattaaaa gcgttattgt
    16861 gtcatgtcaa tttaattccg atgaatcctg ttaaggaaag tcattatcgt gaaagtcacc
    16921 aggaaaggat taactatttc aaaaagattt tagagtcaaa aggtattcaa acgaccataa
    16981 gaagaagatt gggcagtgat attgatgcag cctgcggaca attgaggcga aggtacttag
    17041 aaggaaatgg ggtggaaaaa tgatttcagc agggcaatgc catattggaa gacacagaaa
    17101 caaaaatgaa gatgccattt ttttacacga tcagccatat ggttgtcttc caaatcttta
    17161 tattgttgct gatggcatgg gtggtcatca ggcaggagaa tatgccagtt ctttagccat
    17221 acagtgcttt tgcgaatttg ttagagagca tagagatcat gaaattacgc gggataattt
    17281 aaatgctttt ttacatgatg caattcttta tacgaataat catgtttatt ctaaagctca
    17341 aaatgatccc aattataatg gaatggggac aactttttta gtagctgttt ttctggaaga
    17401 taaaatctat gtagcacatg taggtgacag tcgagtatat cttattcaag gtgaccgtat
    17461 ccgtcagttg accctggacc attcttttgt ggaagaaatg gttcgaaaag gagaaattac
    17521 tgaaaaagaa gccctgcatc atcctaatcg taatatcatt acaagggcag taggaacaaa
    17581 ttctactgtc gatgttgatt tttatacaga agatattacg gatgaaaccc tgaccttaat
    17641 gtgttcagat ggtttatcca atatgttaga tgaagaggaa attttacata tagtaaaggg
    17701 aaacgcggat ttagaagact tatctcaagc tttagttgac agagccaatg aaaagggagg
    17761 atttgataat atttccgtca tcattataag gggaagggaa ggaaggtgag aaaatgctgc
    17821 aaaaaggata tatactaagc aatcgctatg aaataattga aaaaattggt gctggcggca
    17881 tgtctattgt atataaagcg aaatgtaata aattacaacg ttatgtagca ataaaagtat
    17941 taagagaaga atttgtcagt gatgaggagt ttgtgacaag attcagggtt gaagcgcagt
    18001 ctgcagcaag cttgtctcat cctaatattg taaacattta tgatgtcggc aatgaagata
    18061 acctatatta cattgtcatg gaatatattc agggaaaaac ccttaaacaa atgattgacg
    18121 aagaagcacc cttttcttct accaaaaccc tgaagtttgg gttggatatt gcatcggcat
    18181 tacagcatgc ccataagaat aatattatcc atagggatat aaaacctcaa aatatattgg
    18241 taaccgatga aggcgttttg aaagttgcag attttggaat tgcaagagca gttgattctt
    18301 caacagtcgt tacaacgggc aatgcgattg gctctgtaca ttatttttct cctgaacagg
    18361 ccaggggcgg ctttgttgac aaaacaagtg atatatattc tttaggaatt gttatgtatg
    18421 agatgattac aaaaactttg ccttttgaag gagatagtcc ggttacagta gcgcttaaac
    18481 atattaatga agagcttcct gatccgtcaa cggttaatcc tgatattttt ccaagtctcg
    18541 aagatattat tttaaaagcc actcaaaaga aaactgaaaa cagatataaa agtattgatg
    18601 aaatgataag ggacatggaa agagctatag atgagccaac aggaaaattt gtttatatga
    18661 atcaaagaga tttaatagat tcccctacga ttgaaatgac tcaggatgag atgaaaattt
    18721 taagaggaga atatcaaaac aattattcaa aagaacttaa cgggctaaaa gatgatatgg
    18781 atgcagaatt tgaggatttt gataatgagg attccgagga cgaggcattt gaagagaaaa
    18841 atcctaaaga aaagtgggtg gttgcaggag cagtattcac ttcattgatt attattgtta
    18901 ttttaagcgc tgtaggaatt aaaatgattc gcagttattt aacacctaag gttgtggaag
    18961 ttccgtcatt attaaatatg actgttgaag aagcaaaagc tttattagat gaaaggaaac
    19021 ttattcttat tgaagaggaa gaaatatttg atgatgaaat tgaagaaggg aaaatagctt
    19081 ttcaggatcc acaagaagga acggtcatta acattaattc tgaagttgta gtaaaggtaa
    19141 gcaagggtta ccagagtttt gaggtaccag atgtggttga tttagattat gatacggcta
    19201 ttcgtatgtt agaagaatac tttgatgtat ccgttgattc ccaatatagc gatgaagtag
    19261 aagaaaatca tataatttct caggaacctg aagcgggaac tttattaaaa gaaggcgata
    19321 cagtaaaaat taccataagt ttaggaaaag aaatacaaac gattcctgtg cccaaggtag
    19381 tcaatttaag tgaagaagct gctgttaacc aattgcgtaa ttcaggtttg aatgtaggca
    19441 gcaggactta tgtgcctagt gatgaagtag aaaaaggtta tgtcattagt cagagtgttg
    19501 ctccaggtga gttggtaaga gaaggttatg cagtagattt ggtaattagt gaaggttcca
    19561 ataaaactga agaaccagat aaacctactg acaacaataa tacgggagga ccggttgaaa
    19621 aagttataga gattatggca ccaaccgata ttgatttgcc aatagtcaat gtaaaaatca
    19681 gactcaaccg ggaaaacaga tttgtttatg ataaaaatca tgatatttct gagtttccta
    19741 taaaagttcc aattgttgga gaaggcgaag gatttatcga agtctatatt aatgatgtta
    19801 taaaatatag agaaaatata tcttttacta atcctgtaaa tactgtggag gagacagaat
    19861 gattgtagga acgattgtaa aaggtattgc tggcttttat tatgtcagag ttgatgacaa
    19921 ggtatatgaa tgtaaagcta aggggaaatt tagaaacatc aatttaattc cttatgtagg
    19981 tgataaagca gaaataacac ttttaaatga agaaaaccgt ggcagtattg tgaatatatt
    20041 gccacggaag aattacttaa ttcgtccacc ggtggccaat gttgatcagg cagtgattgt
    20101 atttgctgta aaaaatccta atgtcaatct gcagcttctg gacagatttt taattttagc
    20161 tgaagaacaa ggtctggaga ttgttatttg ctttaataaa attgatttag aagaagaaaa
    20221 ggtttgtaaa gagctggcag ccatttataa gaaggctggt tataaaacca tattaagcag
    20281 tacagtatcc aatgaaggta ttttagaact aaaagaagct ttagcggata aaacaacagt
    20341 ttttgctggc ccctcaggtg ttggtaaatc ttctttgctg aatgccattg atacttcttt
    20401 aaaattagca acaggaatga ttagtgaaaa aataaaaaga ggaaaacata cgacaagaca
    20461 tgtagaactg ttacccctgg ataatggtgg ttttgttctt gatacgccag gcttcagttc
    20521 cctttccctt tctcatttgg attcaatgaa actgcaagat tattacaggg agtttgttcc
    20581 ttataataat gcttgcaaat ttataccttg tagtcatata catgaacctc aatgtaaaat
    20641 taaggaaatg gtagaggaag gtctcattga ttttgaacgg tatcagcgtt acaagagtct
    20701 ctataatgaa ctcgaatcca atgagcaaag gagatggaaa aagtgaaaat attaattatt
    20761 gccggcggag atattgagga ttattctttt gccagaagtt atgcggatga ttcagattat
    20821 attatctgtg cagacagggg tataagccat gcaagggcaa tgtccttagt tccggattta
    20881 atcgtaggcg attttgattc tgcttcaaag gaagatatgc attattatca agataaaaat
    20941 atcattttta aaagttttcc ttccaaaaaa gataaaaccg atacggaact tgccctggat
    21001 attgccatag aaaaaaatcc ccgggaaatt tatattattg gtggaactgg aaaccggctg
    21061 gaccatactt tagcgaatat ccatttatta tattatggtt tacaaaagaa gatagctatt
    21121 tcacttataa cttcaactca gaagatttat ttaattgata aagaaattac ccttaaggga
    21181 gaaaagggta attttgtctc acttattcct ttttctttag aagtccatgg tgttacgaca
    21241 aaaggtttag aatatccttt gaatgaagcg gtcttataca gtggcagttc atatgggatc
    21301 agtaatgtga tgactgcatc tacggcaagc atctgcataa aggcgggttt tcttttagtc
    21361 attcaagtat taaaagagta aaggtctcaa atcgattgag accttttttt gcacacattt
    21421 ttataagtat gaataaaata taatgaaaaa attaagggaa gagaaaggat atgaagagca
    21481 ggatacttgg cttatttctt ttttcagttg gaatgggtat gcttttggtg tttcttcttc
    21541 cggggactag ttttgttgtt attactgcca tactattttt atttttagga tatatctttt
    21601 ttaaaaaata ttagtgaggt gaaaaaaatg aggataatgg tagttaaaat gccaaaaata
    21661 ttatctaagc tgattagaaa aatatggggt ataaaaaacg aataaaaaaa acaaagttct
    21721 tctgaacttt gttttaataa ttagttggca cgggtaacaa gattagaacg taagcatctt
    21781 gtacaaacat tcattctttt tttggtcccg ttgtctaata ataccttcac ttttttaacg
    21841 ttaggtttcc attgtttatt acttcttcta tgagagtgac tcagttgtat accaaagtgc
    21901 acaccttttt gacaaacatc acattttgcc atagcactac acctccttta taaccatagc
    21961 cctataaaca caacaaagat attttatcag atactcgaaa agaatgcaag aaaaattcta
    22021 gctatattat tttcaattct attttgaaaa ataataaaag atgatataat atataataaa
    22081 gtttttatat gaaaagaata tactcaggag ggttagtatg tcaggtaaaa ttgtgaatga
    22141 gtacgggaat atttttatag acaatgaagt tattgctcga atagctggtt tagctgctat
    22201 agaatgttat ggaatcgtag gtatggccat aaaaaatgtt accgatggtt tggttcaact
    22261 cttgaaacgc gaaagtctta caaaaggtgt taaagttatc gttgaagaag atgcagtaag
    22321 catagatttt catgttattg ttgaatatgg tacgaagata tcagcagtgg cagataactt
    22381 aattagcaca gttaagtata aagttgaaga atctactggt ttagaagtta agaaaataaa
    22441 tatatttgtc gaaggtatca gagtcgacaa ttaagggtac gaaggaggca gcaaagtgaa
    22501 tatacctaag atagacggag aaatgttaaa acgtatgatc attgccgcta ccaataattt
    22561 aggtaaaaat aagcgcatgg tcgatgaatt gaatgtattc ccggttcctg acggagatac
    22621 tggaaccaat atgtatctga ccattttgtc tgctgcaaga gaagtagaaa aaacagaatc
    22681 taaagatgtt tttgaaattg cgaaagcagc agcatctggt tcattaagag gtgcaagagg
    22741 taattctgga gttattttat ctcaattgtt cagaggtttt tcaaaaggac ttcagggtta
    22801 ttcggttgcg gatacaaagg ctttggccaa tgcgtttgat tcaggggtaa aaacagctta
    22861 taaggctgtt atgaagccca aagaaggaac cattttaacc gtggcaagag gtatggcaga
    22921 aaaagcccag gcacttgcag aagaaacgga taataataat atagagagct ttttacaaga
    22981 tatttttgct catggacatc aggtattaaa aaagacaccg gatatgcttc cagtattaaa
    23041 acaagcaggg gttgtagatg ctggtggaag gggtctttta gaaattttag aaggggcaat
    23101 ctctgcgctt tatatttccg aagacattag tttagtagat aacagtttaa ctgcaaaaga
    23161 tgcaggaact tattctgctt tggcttctgt aagtcaagaa gagattactt ttggttattg
    23221 cactgaattt attataaact gttcagaaaa taatgaaagt gcagcggatg aattaagaca
    23281 atattttaat actttaggag attctatcgt tgtggttgaa gatgatgagg tgattaagat
    23341 tcatgtacat acaaatcacc ccggaaaagt cattgaaaag ggattatcct ttggtcctct
    23401 aaccaatatt aaaatagata atatgagaga acagcacagt agccatctca gtattgaaaa
    23461 agaagaatca aaagcaaaag agtctccttc tgaaaagaaa gaaataggtt ttattgctgt
    23521 atccgcagga gaaggcctta aagaaatatt taaaggtttg ggcgtggatt atgtgattca
    23581 gggcggccag accatgaatc ccagtacaga agatatttta aatgcagttg aaaaagtgaa
    23641 tgcagatacc atagttgttt taccgaataa taaaaacatc atattggctg cagaacaagc
    23701 tcagaaatta tcacaagaaa aaagaattga agtcattcct tcaaaaacca ttcctcaagg
    23761 tattgcagcc atgataggtt atgagccaca caggacgatt gatgatacca tagaatccat
    23821 gaaagagtcc atgtcttatg ttaagacggg acagattacc tttgctataa gagataccgt
    23881 gattggcgac agagaaataa aagaaggcaa tattttagga ttgattgaag gagatattgc
    23941 tgttgtttcc gatgatttag ataaggcaac tttagaactc attgataaga ttgttgatga
    24001 tgatagtgaa atcatcagta tatattacgg agaagatgtt gatgaaagtc aaaccgataa
    24061 aataagaaaa tatatcgaac aagtttatcc ggattgtgaa gtagaaattc attatggtgg
    24121 tcaaccattg tattattata ttttctctgt agaataaaga ttatttccag ggcacccaca
    24181 gggtgccttt ataaaattat gaaaggggtt ttttatgaat ctttccgatt ccgtaaaaga
    24241 gctgaaaggt gtcggagaac aaaccttaaa aaagcttaat agattggata tttataccat
    24301 agaagattta ttatatcatt ttccgcggga ctatgaagac aggtcagaaa ttaaaaaaat
    24361 agaggaaatt acttttaatg aaatcaatac aattatcgct caagtatctg ctttgccaca
    24421 gaatattaag aaaggaaatc ttattctgac caaagtcaga ataaaagatg aaacaggaag
    24481 tatttttgcg gtttggtatg gacaaccata tttaaaaaag cattttaaac caaatgattc
    24541 ttatatcttt accggaaaag ttttatataa atatggggaa attcaaatgg aatctccgga
    24601 atacgaatta aataatggca aagaagttct ttcttttggc agaattattc cagtttatcc
    24661 atcgacctat aagttgtctc aaaagcttat acgtcaaatg atataccagg ccataaaaga
    24721 aacgaagaac cagttaacgg agtttttacc caagtggata aggaaaaaat accaattagc
    24781 agattataat tattccatgc tgaatattca ctttccccag tccagagaaa gtttttttat
    24841 tgccaggaga cgtttggtat ttgaagaatt atttcttctt caaacaggct tgctgagaat
    24901 aaaaaataaa attaaaaata ataaaaaagc cataaaattt gaagatgtgg aagaagtaaa
    24961 tgatttaatt cgccatttgc cttttgaatt aacccaggca cagttaaggg ttataaatga
    25021 gataaagaag gatatgctat ccgacaggtc tatgaaccgc ctggttcagg gagatgtagg
    25081 ttcaggtaaa accattattg ctgctctggc tctttatatg gccgtaaaaa ataattatca
    25141 gggggttatg atggcaccga cagaagtcct ggccaatcag cattatcaaa gcctgctttc
    25201 gttatttgaa aaagaaaata ttaacattgg tcttttaaca ggttctttaa ctaagaaaaa
    25261 taaagagttg ctcttaaaag aaatcgaaga aggcaggatt gatattgtca ttggaaccca
    25321 tgccttaatt caggaaaagg tagcttttaa atctttaggc ttagtgatta ccgatgagca
    25381 gcatcgcttt ggtatggaac aaagaaggct tttattcgaa aaaggcaaaa atccggatgt
    25441 cctggtcatg actgccacac ctataccaag aactctggct ttaatccttt atggggattt
    25501 ggatatttca ataattgatg agcttccccc aggccggcaa aaagtagata cttactgtgt
    25561 caatacatct tacagggaga ggatttaccg atttattaag aaagaattag caagcggaag
    25621 acaagcatat gtgatttgtc cggctgttga agaatcagat attgaaggtc ttcaatcagt
    25681 tattcaatac acagaacaga ttaaaaaagt gtttaacgaa tataaagtct gttttttaca
    25741 tggaaaaatg aaagcaaagg aaaagcagga aatcatggaa gcttttcaaa aaggggagat
    25801 tgatcttttg gtttctacta ctgttattga agtaggggtt aatgttccca atgccagcat
    25861 tatgctgata gaaaatgcgg aaagatttgg tttgtctcaa ctccatcaac taagaggaag
    25921 ggtaggcaga ggaaaacata aatcctattg cattttggtg acggattcag acaataaaat
    25981 aacaaaagaa agaatgaagg ttatgacgaa aagcaataat ggttttgaga tttcagaaac
    26041 cgatttgctt ttaagaggac caggggaatt ttttggtacc cgtcagcatg gcctgccgga
    26101 tttaaaaatt gctaacttat acaaggatat ggatatttta aaagaagtac aggaagcatc
    26161 aaaagaatta ttaagccttg acccagattt aaaagcggaa gagcactact tattaaaaga
    26221 aaaaattaat aaattttttg aagaaaaaac aaaaaatatt tctttataag aatgccataa
    26281 tttactatta tattcgacaa aaaattgcac ataatatgaa tgtaccttaa acaaggtata
    26341 tcaaaaaatg gctgaaatta aaaggaggta atattatggc aagtagaaac cgtaaagcag
    26401 ttcctgaagc aaaagcagcc cttgacagat ttaaatttga aattgcaaac gaaattggag
    26461 tacctttaaa acaaggatat aatggagatt taacttctgc ccaaaacggt tctgttggcg
    26521 gatacatggt taaaagaatg attgaacaac aagaacgtca aatggcagga aatacaactc
    26581 ttcaataata aaagataaaa aggaatggca ttttatgtca ttccttttta tataaaatta
    26641 attacatcaa taaaatatcg atgtaattag ggagaggaaa cagaaagaat atttaaggat
    26701 tcgctattat tatgaacata ataattgact tttattcagt ttattattaa attataggta
    26761 tgaagtaatt tttcttattt tgatagaaaa tgagttttta tgttcaaatt ggaggaatat
    26821 gtttgagagt gattgccgga gttgctaaag gacacaaact gaaagcgcca aaaggaatgg
    26881 atacccggcc aacgacagat aagataaaag aaagcctttt taatataatt gccccagatc
    26941 tttatgattg caatttttta gatctgtata gtggtacggg agccattggg atagaagcat
    27001 taagcagagg agctaaaaaa gcagttttta tagatagatc gcgtatttgc cggaaaatta
    27061 ttgaagataa tttgaaccac acaaaattaa ttgataaggc tgttatttat cagaatgatg
    27121 tcaataaagg attagatatc ttaaaagaaa aaggagaaat atttgacatc attttcatgg
    27181 atcctcctta tttggacaat gatatagaga aaattttgga aaagatttca aagctgaatc
    27241 ttattactcc ttcaggttat attattgttg agcattcttc agaaaaacga atacaagcta
    27301 ttgctgattt atggatatgg aaagaaaaaa tttttaagac gacaaggatg acttttttaa
    27361 aagtagtgga ggaatcttta tgaaaatagc gatttatccc ggtagttttg accccgttac
    27421 caatggacat attgatatta ttttcagggc ttcagaaatg gtagaccact taataattgg
    27481 tgttttgtct aatgtaaaaa aacagccttt attaactgta gaagaacgta ttgatctttt
    27541 aaaagccact acaaaatatt taaaaaatgt agaggtagaa gctttttctg gtttacttgt
    27601 tgattttgct gccaagaaaa atgctacaat tattgtaaga ggcttacggg caatgtcgga
    27661 ttttgaatat gagcttcaga tggctcagac aaaccgtagt ttaaataaaa atatagaaac
    27721 gatatttctg gccaccaaca tccattattc atttttaagt tccagtgttg taaaggaaat
    27781 tgcaatgttt ggcggagata tacatgcatt ggtacctcct gtagttgagg aatacctgaa
    27841 atcaaaattt aagtgaggtg ggatggtatg gatagtattt tacaattatt agaggcaatc
    27901 gaagacatgc tggattcaag cagtgcagta ccttttacag gtaaagtgat gattgataaa
    27961 gacgaattat atgagattat tacggatatc cgtttaaaac ttcctaatga gattaagcaa
    28021 tccaagtggg ttattgaaga aagaaataaa atattgattg atgctcaaaa agaagctgaa
    28081 aataccttaa aagaagcaga tgcaaaactg gcaaaactgg tggacgagca tgaaattaca
    28141 aaaagagcct atgaacaagc tgaagaaatc attgaagctg ccaagcaaaa tgccagggaa
    28201 atgcgtttag gtgctttaga ttatgcagat gaaattttag cagaagttga aaaaagctta
    28261 aagcaagctt tagaagttat acataatcaa aatatggaat tagaagatag tttgggcaga
    28321 gtcattaata ccatttatac caaccgtcag gaattaaaag ggaatgctca aaagtaatcc
    28381 tttgttaatt ttctttagtc cgaaagagtt tgatattaaa gagaataaaa aggatacaaa
    28441 taaaggcaca aagataaggt ccgcttttga accagaagct gttagtgata ttgtaagaaa
    28501 taggattaaa aaccgtttgt gtataagcag atatttttgt catttgggga tagaaggtta
    28561 cagaataaat aaatgcaaaa aggccttgca gtagttttgc acaggcatag atgggaatgt
    28621 taatatccga agaactaatg acactgacag tctgcatgaa tacagacaga cctccaaagg
    28681 caattaaaaa gctgactaat gcaagttctt ctttaagact cgctgaacaa agaccaatcc
    28741 tgctgatacc attggtgatt tcaataatcc ccagaagaaa tgcacttatt agtttgggac
    28801 taaaaacaat cagcttaaaa aatggtttaa acaatttagt gaataaatct aacagggaag
    28861 ataattcaag caattgtgat gtgactgaaa aaaggatgac aaagccacct atttgaacaa
    28921 cagtatccat ggaagacata atgctttctt taagaattaa acctattttt tttgagttgt
    28981 tcttttgaat gagggaaagt tgttggaagg atttcttaat aatatttttg tatttttttc
    29041 catgtacttt tttttctttt ttatagaatc taaaaagcaa gccaacggtt aaggaagaaa
    29101 gatagtgaat agctaataaa aaaaaccctg tttcggcttt tgaaagcatg cctgcagaga
    29161 cggcccctaa aataaataag gggccgctat tatttgtaaa agctataagc ctttgggctt
    29221 caacctggct gatctttcct ttacaccgta aatcagcagt tatttttgcc ccaatgggat
    29281 aaccggaaat aattcccatc atccagggaa aggcgccttc tcctgggaca ttaaaaatag
    29341 gtcccatcac aggttcaaac aaaataccga taaagtgaac gactcctaat tcaattaaca
    29401 gataagtagc aaccataaaa ggaaataaag aagggaacaa ggtattaaac cataattgta
    29461 ttcctctttt agaagcctcc agtacttcct ggggaaagat aataatgcca gttgttaata
    29521 aaataataat gcagacagta ataagtctgg ataaagaaag cgtatcctta tatctcatca
    29581 tatccctcaa ttttttaaaa ataactctta tataaaatat gtttcaggag ggataatcat
    29641 acgtgtgttt tatttattga tgatgattaa aggcatagaa aattcgatgc cgctttttct
    29701 ttgacaaaga tttggatagt gaaggctgta aatatctgtt gactgaattt ctttttcaag
    29761 cattttttta ggaagaccac taaaatccct gtaggctctt cttacattag aaataatagg
    29821 gagttttgaa tttatcttta gcttttttag taaaggtgct gcactttttc taaagccaag
    29881 aattcttata tactgaggtc ccccgtgtct atgaagttcc caaaagtctt cagaataaat
    29941 ctgcaaaaga atatggagca aggtcctttg aattcttgtc atcgtatatc tttttgtttt
    30001 aaccgattca atgatctctt taatgtaaaa atgattcatg gcagctttat ttattctgtt
    30061 ttcaatacct tcatccactt ccataatttt tttaatttct tccataggca tacttctaag
    30121 gaggtattgt aattcaaaag aaaaatcatc gtaataaaca ggagccttgc ccaaatggat
    30181 ggcttccaaa aataaatcag ccgctttttt tggcatggtt ttgtataata tactatggtc
    30241 tttattctta agggcatgcc ggatgcctgt agctgaagca aagagcgtat ctaactgggt
    30301 tgaatggtat gaagcccctt ttcttttaat ggtaaaaggt ttaataggac tgtttaagcg
    30361 ttttaaagct tttaaatatt caatggctaa aatattattt ggggaagaaa gtacctggct
    30421 taattcttct acaggaatgc ttgtattttt atctgacata tcgctgatta gggccttatt
    30481 tcttgcactg gggaaaggaa ggccttccga caaatattct ttaagtttca ttttataatg
    30541 ctctgattcc aagaataaaa aactggctat ttgtgataat aaatctatag agcctgattc
    30601 gctgccaaag gataagtggt caacaatatt tgaatgatgc aggatggata ttgctccaaa
    30661 ggcaaaaaac tcagcgctgg atgtggcata tataagcggt aattcaagaa ccaggtcagc
    30721 tcctgattct aaagccatct gtgttcttgt ccacttatca acgatagctg gttcgcctct
    30781 ttgaacataa ttgccactca taacgacaac ggtaaagtca gaatggctga ttcttttgga
    30841 ttcttctaaa tgatacaaat gtccattgtg aaaaggattg tattcagcaa caatgcctaa
    30901 tactttcata atcattctcc ctatcaagat gataattata ttattctata tgccatattt
    30961 ttatgtcaat atagctgtcg aaaaactaaa aaaaaagata aactttcctt aagactattg
    31021 tatacaataa gcagtttgtt tataatagtt agtggaaaat tattgagagg agtattgttg
    31081 tggactttat acaaacaatc aagcaaagag caaaaagtga caaaaaaact attgtattgc
    31141 ccgaatcatt agagttaaga actttaaagg caacagagaa aattttaaaa gaagatatag
    31201 ccaatattat tcttattggg aaagaaaatg agatcttaga aaaagcagaa ggactggata
    31261 tctccggagt taaaatcata gatccggaaa actatgccca aatgaatgat tttattgata
    31321 cattagttga attaagaaaa aataaaggta tgaccagaga aaaagcagaa gagcttctta
    31381 aaaatccttt atattttgct gtgatgatgg taaaaaaagg catagcagat ggcatggtag
    31441 caggtgctgt aaattctact gctgatgttc tcagaccttc tctccaaatt ttaaaaacgg
    31501 ctcccaatac aaaattggtt tctgcatttt ttgttatggt tgttccagat tgtgaatatg
    31561 gagcaaatgg aacttttatt ttctcagatg ctggtttagt tccaaatccg aattctgagg
    31621 aattagctgc gattgctgtt agttcagcaa aatcctttga acatttagta gctactgaac
    31681 ctatagttgg aatgctttct ttttcaacaa aaggcagtgc ttgtcacccc gatgtagaca
    31741 aggtgattga agcaaccaaa atagctaaag aattggctcc ggatttaaag atagatggag
    31801 aattacaact ggatgcagcg attgtaccaa gtgtaggcgc aagtaaagca ccaggcagtg
    31861 aaatagctgg taaagccaat gtattaatct ttcctgattt agatgcgggt aatattggct
    31921 ataagttgac tcagagactg gcaaaagctg aagcttatgg tccaataact cagggaattg
    31981 caaaaccagt caatgatttg tccagaggtt gcagcgatga tgacattgtt ggtgttgtag
    32041 ccattacatg tgtacaagca caaattaatg aaaattaata atacaatata aatccaatta
    32101 ttgtatttcc attctaaata ctttattaaa gcactgaagg aataaaaaat aaattttata
    32161 taaatattat atggggagac gataataatg aatattctag ttattaattg cggcagttct
    32221 tctttaaaat atcagttgat taatatggtc gaagaaaaag tatatgcaaa aggaatatgt
    32281 gacagaatcg ggattaaagg ttctgtactt aaacatactc cggctggcaa agatacagtt
    32341 atgaaagaag tcgctatgga aaatcataaa gatgctgtaa gagaagtttt gtctgcatta
    32401 actcatgaag aatatggggt tatccgcagt atggatgaaa ttgcagccgt tggtcacagg
    32461 gtagttcatg gaggagaaaa attcagtgct tctgttatta ttgacgatga agtgattaaa
    32521 gccattgaag aatgtagtga tttggcaccc ttacacaatc ctcccaattt aattggaatt
    32581 gaagcttgca gaaatttaat gccagataca cctatggtgg cagtttttga tactgcattt
    32641 catcaaacca tgccgagtcg ggcatattta tatgctattc cttatgaatt atatgaagag
    32701 cataaaatcc gcagatatgg tttccacgga acttctcaca aatatgtggc acaccgggct
    32761 gcggagatgt tgaataagcc aattgaagaa ttaaagattg ttacatgcca tttaggaaac
    32821 ggagcaagta tatgcgcagt aaaaaatggt caatccattg ataccagtat gggatttaca
    32881 cctcttgcag gtctggtgat gggaacccgt tgcggagata ttgaccctgc aattgtagga
    32941 ttcttaatgg ataaagaaaa tatcagctca gaagaagtag atgttatttt aaataaaaag
    33001 tccggaatac ttggtttatc aggtgtatcc agtgatttca gagacattga agctgctata
    33061 agcaacggaa ataaaagagc agaaattgca atggaagtat ttgtttatac tgtagcaaag
    33121 tacataggtg cttatgcagc tgccatgaat ggtattgacg ttattgtatt tacggcaggt
    33181 ttaggtgaaa ataatagtga agtaagaaga gatgtttgca aatatcttga tttcttaggt
    33241 gtaaaggttg atgaagaaaa gaatagatgc agaggcatag aaagagattt ttctgctgaa
    33301 gatgccaagg ttcgtacttt ggttatacca accaatgaag aattgatgat tgcaagagaa
    33361 acaagaaact tattaaaaaa ataatttatt gacaaatatt atacctctat gtataatatc
    33421 aagagtgaac ctgcagaggt gatatcatga tacttaatgt atctaaaatc attaataatc
    33481 ctggctcctc aatggaatta tcttttgatg aaaagattga ttttattgag tgtggcaagg
    33541 aaaaaattaa gcttattgaa ccagttcgat ttgttggtga aataagctgt ggagatggaa
    33601 aagagcttta tctgacgggt aaactaaata cccgtataga attgccttgt agccgctgtt
    33661 tacaaccagt ggaagtaacc attgaattgt ctttaaaaga atattttact aaaaaaggca
    33721 atctaaaagc ggatgatgaa gagtttcatc cgatagaggg aaatgaaata gatttaggac
    33781 ctgtttttat taaagatatt ttattaaata ttcccatgaa aaatctttgc aacgctcatt
    33841 gtaaaggctt atgtccaaaa tgtgggcaag atctgaacaa aggaacttgt aattgtaaag
    33901 aagatgtaat tgatgagcga tttgctgttt taaaaacctt atttaacggg tctaacagtt
    33961 aacctaagga ggtgtaggaa atggcagtac caaagggaaa agtttcaaag gcgagaaaaa
    34021 ataagagaaa agcaaactgg aaattaactg ctcccaattt agtaaaatgt gagaagtgtg
    34081 gagaattaat gatgcctcat agagcatgta aatcatgtgg aacatataat aataaagtgg
    34141 tagtaaacgt agaataagat taaacagaca gcttttgctg tcttttattc tatataaata
    34201 tgatttaaag cctattaggc cttaggcgga atggaggtta agatgtctta caaaacagtg
    34261 attgccctgg atgcaatggg gggagacaat gcaccttctg caattgtaaa aggagctgta
    34321 ttagctgcca gagaggatat aaaaattatt ttagttggag atgaaaataa gcttaaaact
    34381 gaattaagcc agtatggtat ggattcatct agattatcca ttcatcatgc ttcagaagtc
    34441 attaccatgg atgattctcc agtggctgcc ataagatcaa agaaagattc atctatggtt
    34501 gttggtttaa aattattaaa agaaaaagaa gtggatgctt ttatatcagc aggaagtaca
    34561 ggagcccttc tcgcaggagg aactttcttg gttggaaggg caaggggaat tgaacgacct
    34621 gctcttgcgc ctttaatacc aaatataaaa ggtttttccc tattaattga ctgtggggcc
    34681 aatatggatg ctaaagccag ttatttagcc caatttgctt taatgggttc catttatatg
    34741 gaaagtatta tgggtattcc ttctcctaag gtcggcctta taaatgtagg aactgaaaat
    34801 gaaaaaggga atcagttaac aaaagaagct ttttctctat tggaaaaatc agatattaat
    34861 tttataggca atattgaagg cagggatatt agtatgggtg tcgcagatgt cattgtctgt
    34921 gatgctttta cagggaatat tattttaaaa catacggaag gtttagccct ttcattgttt
    34981 aatattatta aagaagaact tatgaatgat aatctttcaa aaattggtgc tttgctttcg
    35041 aaatctgctt ataaacgggt taagaaacgt tttgattata cggaatatgg cggagctccc
    35101 atgcttggtc tacaaggctt agttgtaaaa gctcatggaa gttctaatgc aaaagccatt
    35161 caaagtgcta tagcacaagc ggttaagttt aaagaagaaa aaattgtaga aaaaatcgaa
    35221 gaaaaaataa atcaaaaaat taatcaataa ctattttaat attgacggtt ataagagtat
    35281 atactattat taatgtataa taaataataa taaatggggt gatagggtgg tatttgagaa
    35341 ggttaggcaa attattgctg aacagttaaa catatctgaa tcagaaatta ctgaagacac
    35401 ttcatttcaa gatgacatgg gtgccgattc attggatata tttcaaatta taatggcatt
    35461 agaagaagaa tttgacatag aaatttcaaa tgaagacgca gaaaagattt ctactgtcaa
    35521 agatgctgtt gaatatattc aaaatatgat atccgcagaa taaaagtcct taaaaaggac
    35581 ttttgttttg taataaggaa gttcttacga ttaaaataat tggcaagatt aaggagagat
    35641 aaagattgct aatccttttt gaaggtgaaa tgaatggaaa aagataattt agagctcata
    35701 gcggaatttg agaatattat tggttataaa tttaaaaata aaaaatggat tatggaggct
    35761 ttaacccata gttcctattc taatgaaaat aaaaagaaaa atcttactaa taatgaacga
    35821 ttggaatttc ttggagatgc catattagat ttaattatta gtgagtatct ttttaattta
    35881 tatcctgatt tgccagaagg tgagcttaca aaattaagag ccagtattgt ctgcgaatta
    35941 tctttatcaa cggttgcaaa caaatataat tttggcaagt atctttttct gggcaagggt
    36001 gaagaattaa ctggtggaag agaaaggcct tctatccttg cagatgcttt tgaagcaatt
    36061 ttgggtgcgg tttatattga tggtaaaatg gaaagtgctg ttgattttat taaaagaatt
    36121 ctaattcctt caataaagaa taatcaagct ttcagagatt ataagacgat gctgcaggaa
    36181 gaaatacaaa aatcaagcca tgaacctata gaatacagta ttatatcgga atcaggtcca
    36241 gaccatgaca aagaattttt tgtagaggta aagcattcga atgttaccct tggaaaggga
    36301 aaaggaaaaa gtaaaaaaga agcagaacaa aatgcagcct ataacgcatt aaatgcttat
    36361 caggacaaaa aagaaaagtg atttttgtac tcggataaga ggtgaatata tgtacttaaa
    36421 aagattggaa attcagggct ttaaatcttt tgctgatccc ttaaagttgg attttagtaa
    36481 agaaataacg gctgttgtcg gtcccaatgg aagtggaaaa agcaatattg cagatgctct
    36541 tcgctgggta ttgggggaac aaagtcctaa aacccttaga ggagcgaaga tggaagatgt
    36601 tattttttca gggactcaaa accgtaaacc tttaggtttt gcagaagttt ccatcgtttt
    36661 agataattct gaggggaaaa ttcccattgc ttttgatgag gtaagaatta ccagaagggt
    36721 atatcgttca ggagaaaatg agtactttat taataaatct tcctgtcgct taaaagacat
    36781 ccatcagctt tttatggata cgggtgtagg aaaagaaggg tattctatta ttggacaagg
    36841 tcaaattgac cgaatcctca gtacaaagcc tgaagacaga agatttttat ttgaagaagc
    36901 cgttggtata gtaaagtaca aacacagaaa actggaagcc gagaaaaagc ttgaacaaga
    36961 aaagcaaaat cttatccggg tagaagatat tatttctgaa ttagacaagc aattaggtcc
    37021 tttagcttct caagctgaaa aagcaagagt ctaccttgaa ttgaaagaaa aagtgaaaaa
    37081 attagaatta aatttattta tttatgaggc agacaatcaa aagaaggaat tagacagtct
    37141 ggaagaaatg tttttaaaca atcaggaaga attgtcccgt caaaaagaaa ttcaattaaa
    37201 agataaagaa aattataccg ctctaaaaag agaattagaa gatattgaaa ataatatata
    37261 tgacaatcaa aacagcattt cagatatcag agccaatatt gaaaaaacag aaggaaataa
    37321 caaagtatta gaacagcaaa tagaacatta cattttggat attaaaaggc ttaacaatga
    37381 atctgagaaa ttggataaaa gaatagaggg acttagtcag gaaaaaatag agttgtcagc
    37441 aaaattcaat gcgctttcct tagaattaaa aactaaagaa aaattattgc aagaagaaga
    37501 aaatcatttt agcgggctca tccatttttt gctcacagga gaatctgaaa ttgaaaatta
    37561 caaatcggat atcatcgaaa aaatgaatga gtcttctgaa ttgaaaactc ttattcaaaa
    37621 aatcgacagt gaaattcaat atatagaaga aagaaaaaag caaattttat ctgaaaaaga
    37681 actgttggac agacaataca gggaacaaag cgtacattta aaagctataa agaaactgga
    37741 agaaacatta aaagaagaaa ggaaaaaatt aaatcagaag attaatgaat taattagcat
    37801 taataatcag ttcattgcgg atgttcaaaa agaaagcact gctctgaatg atttaaagga
    37861 agaaatgcat agcattaaat ccagaaaaga tgttctggaa ttaatggaaa atgaatatga
    37921 aggttttaat aagagtgtaa aaagtcttat gcgcttaaaa aaagataagc ccaatcagtt
    37981 aaaggggatt tgcggggttg tagctggttt aatcgaggta cctaagggtt atgaaacggc
    38041 aatcgattta gctttaggaa acagtctgca aaatattgtt accgaaacag aagaagatgc
    38101 caaagatgcc atagattatt tgaaacaaaa caatttaggc agggcaactt ttttacctct
    38161 ttctgctatt aggggtaaga gcctgggggc cgatgaacag aaaattttaa atgaacaagg
    38221 ggttttaggc attgcttctt caattgttca ttatgatgaa ttgtataaag atgttatttc
    38281 atatctttta ggcagggtca ttatcgtgga caatctgaat catgccattt ggattgccaa
    38341 aaaatatcag cacaaatata aaattgtaac gatagatggg gaagtgctta atcctggtgg
    38401 agccatgacc ggaggaagta tccagcaaaa aagcatgaat atctttttaa ggtcaagaga
    38461 attacaagag ttaaaagaaa agcttataaa attgacagaa gaaattaaaa acagggaaga
    38521 aagaatcaga gcctatgaag ataagaaaaa tcaaaacctg gctaaaatag aagatttaaa
    38581 aagcaaaatc caggaactgg atattaatat actatccaat cagcatgaga tagataaatg
    38641 cgaagaaatg ctcaatcaat taaaggataa ggaaagtcag ttaaatttag aagaagaaca
    38701 gattaatatc cagcataaag aaaatacgaa tagtttattt gaaaaacggg aagaaatgag
    38761 ccgtattgaa aatgaaataa aagccattca gtctaagatt gaaatgaatc aatctaagct
    38821 ggagaaggaa aaagaagcca aagatatctt aacagacaag atcacaaaat taaaaataga
    38881 aatttcttcc ttagagcaaa aacgggatta catattggaa aacaaaaagc ggctggatag
    38941 tgaaattgaa aagcagaaag ctgagaaaaa acaaagagaa gaagaaattc aaggtatttt
    39001 aaaagacaaa gcattaaagg aaaacgaagt caatgaaaat gtccgggaaa ttgaaaagtt
    39061 aagacaaact ttaattgaaa agaccgggca gcaagaaaga tttcaggaag ttaaaaaaga
    39121 aaaactggct gtatataaag ctttagaaga aaaaattgaa gaaagttcca aaaccattga
    39181 attacttcaa acagaaatga atcgcctgga gaataagaag actaaattaa aattagaaca
    39241 agaagtttta tacaataaga tttgggaaga atatgaggaa acttaccatt ctgctttaaa
    39301 atataaaagt acagaattat caatttcaga tatgaaaaaa cttttagcaa agcataaaaa
    39361 tgaaatgaaa gatttaggta atgtgaatat aaatgcgatt gaagaatatg aaaaggttaa
    39421 agaacgttat gatttcttaa cagcccaaag agaagatatt ttaatggctg agaagaaatt
    39481 gtttggcatt ataaaagagc tgacggcttc gatggaaaaa caatttattc aaaattttgc
    39541 tacaatttct gctaacttta atcaggtatt taccgaactc tttgctggtg gtaaggctta
    39601 tttgaagctg acggatgaag aaaatatatt agaatccgga atagaaatta ttgcacaacc
    39661 acctggaaag aagttgcaga acatgatgct tttatccgga ggagaaaaag ctttaacggc
    39721 cattgcactt ttattttcta tattaaagat gaagccttcg ccattttgtg ttttagatga
    39781 aatagaagct tctctggatg atgcaaatgt catgcgcttt gccagctatt taaagaaatt
    39841 tagagatagc acgcaattta ttattattac tcacaggaaa ggaaccatgg aggttgcgga
    39901 taccttatat ggggttacaa tggaagaaca aggtatttca aaattactgt ctgttaaact
    39961 ttcagacata gaggaaaatg aagaagcaag ttaaaggagt ggaataatga gcttatttga
    40021 caaatggcta aacagagata aaaacaaagg acaggaagaa tccataccag aagaatccca
    40081 gttggaggaa ttcgttcatg acaaaactga ggaagatata ggatttagcg aagaaagtta
    40141 tgaaataaaa gaaagcgtag aggaagaaga aaatccgcca gaagaagaag ggcaggaaga
    40201 aagcgttaaa gagaaaaaag gtttcttttc gaagctggtt aaaggactgg ctaagacaag
    40261 aaacaatatc atggatggta ttgataatgt tttaaagtct tttaacaaaa ttgatgaaga
    40321 attgtatgaa gaattagaag agacacttat tatggcagat ataggtgtca atgccagtat
    40381 aaaaataatg aatgggctaa gagagaaggt caaagaaaga aaaataaacg agcctgcggc
    40441 ccttaaacaa ttgttaattg aagaaatcac tgaaatactt agtaaaggga accgggactt
    40501 aaatattcag gaagggcctg cagtgatttt agtcattggt gttaatggcg taggtaaaac
    40561 aacaacgatt ggcaaactgg cccatctgat taaaaaagaa aataaaaagg ttcttttggc
    40621 agctgctgat acctttagag cggcagccat tgagcagttg gaaatatggg gaaacaggac
    40681 aggagtcgat gtgatccgtc atcaggaaaa ttccgaccca ggtgcagttg tttttgatgc
    40741 aatacaagcg gcaaaggcaa gaaaaattga tgtcttaatt tgtgatacgg ctggaagact
    40801 tcataataaa aagaatctca tggaagaact tagaaaaata tttaagatta ttaacaagga
    40861 atatcctgaa gcaagaaagg aagttttatt ggttttagat gcaaccacgg gtcaaaatgc
    40921 ccttcagcaa gtaaaattat ttaaagaagt gacggatatt tcaggtatta tactgacgaa
    40981 actggatgga acagctaaag gcggcattat tattgccatt aattccgaaa tggatattcc
    41041 tataaaatat attggtgtag gagaaggcat agaagattta cgtcctttca atccaaaaga
    41101 atttgcagaa gctttattta cggatgaaga ataaaatatt tcttgacaaa caacagcctt
    41161 tactataaaa tacatctgta aagtatataa gctttacact ttccaaaaac gtggtggtaa
    41221 catggaaaaa cttttgcaaa ttaccctgct ttatgatttt tatggagaat tactcacaga
    41281 caagcaaaaa aatatatttg agttatacta tcaaaatgat ttttcacttg ctgaaattgc
    41341 agattacctt aaaataagca ggcagggagt ttatgatacc ttaaaacgca ctgaaaatat
    41401 tctttatgat tatgaaaata aattaaaact ggttgagcgt tttgagaaaa ataagactta
    41461 tattaaagaa attgtcaata cgataaatga agctgaaaaa cacctggatg atttaaataa
    41521 agttcatata tgttttgctg aattaaagcg aattgctaat caattaatta ttgaatgaat
    41581 tggaggcttg ccctatggct tttgaaagtt tgtctgacaa gcttcaggaa gtatttaaaa
    41641 aattaaaagg taaaggaagg cttacagaaa aagatgtcaa tgaagcctta agagaagtta
    41701 aacttgcttt attagaagca gatgtcaatt ttaaagtggt taaatctttt attaataaaa
    41761 ttaaagaaag agctgttggg catgatgttc ttgaaagtct tactccagga caacaggtga
    41821 taaaaatcgt taatgaagaa ttgacggagc taatgggaag tacccaaagc aagttaactt
    41881 tttccaatac accaccgacg atttatctga tggtagggct tcagggttca ggtaagacga
    41941 caacttccgg gaaactggca gcccaaatta aaaaaatggg caaaaagcct ttattggttg
    42001 cctgtgatgt ctatcgccct gcagcaatta aacagttaca agtggttggt tcaaaagttg
    42061 gtgttcctgt attttccatg ggagacgcaa ccaatcctgt cgatatcgct aaagcagcag
    42121 tttcctatgc aaaaaataat aatcatgatc tggttatcat tgatacagca ggccgtcttc
    42181 atattgatga agaacttatg aatgaattgt atgagataaa agaagtgaca aagcctcagg
    42241 aaattctttt ggttgtcgat gctatgactg gtcaggatgc tgtgaatgtc gctgaaagtt
    42301 ttaatgaaaa attgggcatt gatggagtaa ttttgacaaa gcttgatggt gatacaagag
    42361 gcggtgctgc actatccgtc cgtgctgtga cccagaaacc tataaaatat gttggtatgg
    42421 gtgaaaaatt agaggattta cagccttttc atcctgacag gatggcttca cgtattttag
    42481 gtatgggcga tgtattatct ttaattgaaa aagcccagga aaactttgac caaaagaagg
    42541 cccaggaatt agaaaagaaa atcagaaaaa tggaatttaa ttttgaagat tttttagaac
    42601 aaatgcaaca agttaagaaa atggggcctc ttaatcaact tataggaatg ataccaggca
    42661 tgaatatgca aaagcttggt aatgtagaaa ttagcgataa agaaattgcc catatcgagg
    42721 cgattattca atccatgact ctggaagaaa gaagaaatcc tgaaatcata aacccttcaa
    42781 gaaaaagaag gattgccgct ggtagcggaa gggatataca agaagttaat cgcttaatca
    42841 aacaatttga acaaatgaaa aagatgatga aacaattatc gggtatgacc aagggcaaaa
    42901 aaggaaaatt taaacttcct tttatgtaat atattcattt gatactgatt aaggggaggt
    42961 gaaaaacatg gctgttagaa ttcgattaaa aagaatgggt gcaaaaaaag cgcctttcta
    43021 tagaattgtt gtagcggatt ccagagctcc aagagatgga agatttattg aagaaattgg
    43081 atattatgat ccaacaaaag atcctgcagt gattaaagtg gatgaagaag ctgttcaaaa
    43141 atggcttaaa aatggcgcac aaccttcaga tacagttaga ggattgctaa aaaaagctaa
    43201 cgtattgaaa tagtaagaat ggaggtttaa gttatgagag aacttgttga agtcattgca
    43261 aaagctttag tggataatcc tgatgcagta caagtcaatg agttagaagg agaacgctcc
    43321 gttattttag aactgaaagt tgctcccgaa gatatgggaa aagtaattgg taaacaagga
    43381 cgaattgcaa aggctatcag aacagttgtt aaggctgctg caattcgcga aaacaagcat
    43441 gttgttgttg aaattttaca ataagtcaaa ggttaggaat tttcctagcc ttttttgcag
    43501 acttttatag aataccagcc aatagatgaa aaaaagctta aggaggattt ctattgtcca
    43561 attacatgga aattggtaag attgtaaaca cccagggaat ccggggagat ttaagaatta
    43621 ttcctacaac ggatgatcct aaacgctttg aattgctgga tactgtatgg gtcgaaagca
    43681 aagaaggaac aaaggaatat gaaatagaaa aagtatggta tcataagcag tttgttattt
    43741 taaagttaaa aggaatatct tcgattaatg aggctgaaaa actaaagaac tctattgtta
    43801 aaataccaag agagctggcc ttacccttag gtgaaggcga atattatatt ggagatttat
    43861 atgatttgga agtctatacg aacgatgagg aatatttagg taaaattgtg gatattcttt
    43921 ttacagctgc caatgatgtt tatgtcgtga aaaaggaaga ttcgaagaaa gaattgctta
    43981 ttcctgctat aaaacaatgt attttagagg tggatttaaa taatcggcgg atgacggtca
    44041 atttattgga aggattgagg gaactatgaa tttttatatt ttaaccctct tccctgatat
    44101 gattgagtct gccatgtctc atagtattac tggcagagct caggaagcag gaattattaa
    44161 aataaattgc attaatatcc gcgattttgc caataataaa cataatcaag tggatgatta
    44221 tccatatggt ggagggccag gtatggtact ccagccccaa cccgtttatg atgcctatca
    44281 aagcattatt ttaaaaaaga atataaaacc ccgggttatc tatatgacgc ctcaaggaaa
    44341 aattttttcc caggctgttg caaaagattt agctaaggaa aaagatttaa tgattatttg
    44401 cggacattat gagggaatcg accaaagagt gattgatgaa attgtgacgg atgaaatatc
    44461 tattggcgac tatatattaa caggagggga attagcagct atggtcgtta tagatgcggt
    44521 ttccaggcta attcccggag tgcttggcaa agaagaatcg tatcaggatg aatcctttag
    44581 taatcatttg ctggaatatc cacaatatac gagaccttta gtttttagaa acaggaaagt
    44641 ccctgaagtt ttgctttcgg ggcatcatgc aaaaatagaa aaatggagaa gagaacaatc
    44701 gctaaaactg actttcgaga aaagacctga tttacttgag aaagcagagc taacagagga
    44761 agataaagcc tatctggata gtttaagaaa aatgtaaatt ttttaaatgt tttgttgtaa
    44821 ttaaattaat attgcaaata aagatataat atgctatact ttcataatgt gagtacggac
    44881 ggtcctctgc ttaaaatact aagtatgaac gtctaagatg gaaggagggt acttcatgaa
    44941 cgaaatcata aaagaaatag aaaaggaaca gttaaaaact gatattacac cttttgaagt
    45001 tggagatact atccgtgtat attctaaagt aaaagaagga aaccgtgaaa gacttcaaat
    45061 gtttgaaggt attgtaatca aaaagcagca tggaggtatc agagagactt ttactgttag
    45121 aagactttct tatggtgttg gggtagaaag aacatggccc cttcattcac caagtattga
    45181 tagaatagaa gtggtaagaa aaggtaaagt aagaagggct aaattaaatt atctccgtca
    45241 aagagtgggt aaggctgcaa aagtaaaaga aatcatcgaa taaaaaaggg gactaaccag
    45301 tccctttttg tgtacttgaa agagggggaa ttatggtttt tatggaaaac aaagataatg
    45361 actctgctga aagtaaagaa aaaacagaaa agattaataa gaattttaag gaattattag
    45421 aatttataag agatttaatc attatatttg ttcttgcaat gtttcttaga acttttgttt
    45481 ttgaaaatac aagagttctt ggtccttcca tggaaccaac tttacatgat ggagatctta
    45541 taattgtaaa taaattagaa tattatttta cagatccgga aagaggagaa ataattgttt
    45601 ttccttataa aggagaccct tcaaaacaat acattaagcg cattattgct ttacctggag
    45661 aggttattga tataagagag ggtaaagttt atattaatgg agagctttat gaagaagaat
    45721 atattctgga agaaatgcaa ctgcggggag atatagattt tccatatgcc attccagcag
    45781 atacgtattt tgtaatggga gataatagaa ataacagttc ggacagccgt tatcaggatg
    45841 ttggtaccat tgaaaaagat aagattattg gtcatgctgt ttttcgttta tggccaatta
    45901 ataaatttgg acaaataaaa taaaattgtg agttgtgatt gggatatgaa tgaagctgta
    45961 aataagaaaa tcataaaaga gcttttcgaa tggttaagag atattatcat tgctgcgttg
    46021 gttacactat ttattataaa ttttttattt caaaataccc aagtgattgg agactcaatg
    46081 gaaccatctt tagaagatgg tcattcagtg attatcaata aatttattta tcgttttaaa
    46141 gcacctcagc ggggagatat tattgccttt aaaagtcctg tgaatccatc ggagcatcat
    46201 attaaacgaa ttataggaat tccaggagat acggtagaaa ttaaagaagg caaagtttat
    46261 ttaaatggca ttcttttaaa ggaagactat attcttgaac caatggatgc taaaatggct
    46321 ggggacatgg aatatcctct aatgattggt ccggatagtt attttgttat gggcgataat
    46381 agaaatataa gttatgacag tcgttataaa gaaataggtt taattgacga atcttcaata
    46441 agtggtaaag ctttaatacg gatatggcca ttaaataaaa tcggaagggt taaataagat
    46501 tttggaggtg attatatggg gcaaaaaaac aagtctttca tatttaatga agcaatgagt
    46561 tggctaagag atattataat tgctgttatt attgccttat ttgtcaggca atttgctttt
    46621 gctcatacag ttattcccac tggctctatg atcccaacga ttcaaatcga tgatcatatt
    46681 tttgtaagta aattgtctta ttatttttca gatcctcaaa ggggagatat tgtcgtattt
    46741 catcatgatg ttgatttgat taaaagagtc attgctttgc caggagaaga aattgatctg
    46801 agagatggca aggtttatat taacggggaa cctttagaag aagattacct taatgaacct
    46861 atggataccg gtataactgg atttctggaa tttccttata aagtccctaa agattgttat
    46921 tttgtcatgg gagacaatcg caattatagt aaagacagca gatatttcgg acctgtccat
    46981 agggatcaaa tttttgctaa agcaggtttt agaatttggc ctttgaaatc ttttggtttt
    47041 gtaaaataat tattttgtaa agacggtgaa atcatgaata tacaatggta tcccggacat
    47101 atgtcaaaaa caagaaaaat aattttagaa aatttaaaac ttgtggatct tgtgattgaa
    47161 ctattagatg caagaatacc ttatagcagt aaaaatcctg atattgatgt tttagctgca
    47221 aataaaaata gaattatcgt catgaataaa agtgatctgg ctgattctaa tatcacttct
    47281 ctttggattc aacatttcaa gaattacggc tatcatgcca tttctgtcaa ttcaatggat
    47341 ggaaaaggaa tttcccaagt tgaaaattta gcaagagaat tattaaaaga aaagattgaa
    47401 aaggataaga aaagaggtcg cattttcagg ccgatccgtg caatggtcgt tggtattccc
    47461 aatgttggaa agtcgacctt cattaataag ctggtaggta aggccagtgc aaaaacagga
    47521 gacagacctg gagtaaccaa aggaaaacag tggattaaaa taaaaaagga ctttgaatta
    47581 ttggatacgc cgggaatttt atggcctaaa ttcgaagatc agacagtcgg tttacattta
    47641 gcttttattg gctcaattaa gcaagaaata ttggatagcc acaatttatc tttagaactt
    47701 ttagcgttat tggctaaaaa atttcccgat tgtttaagcg agcgttataa aataacagat
    47761 attgatagca aaagtccaat agagcttctt acagaaattg gccaaaaaag aggctatttg
    47821 ataagaggcg gggaagtgga ttattccaga acctctttaa tgcttttgga tgaactaaga
    47881 tcaggggctt taggacatat ttcacttgaa tctccggaag agttttatta aattaaatga
    47941 gtatagccaa taatattttc taatgctggt gataaaatgg aaaagttttc tttacaatcg
    48001 atagaaaaac aactcaagga agtttcttat gatgagcttc ctaatttttt agaagaaatt
    48061 aaaaaagatt caagaattgg tgtgcaaaaa ttagtccaaa aatatgaaag aaaatatgag
    48121 acttatcagg tagaattaaa acggattcag gaaatgagtc aatatgaaaa gtattattat
    48181 caaaaaggtg taaaatacat tgccgggatt gatgaagcag gcaggggacc tttagctgga
    48241 ccagttgttg ctgcagctgt tatacttccc aaagattgtt ttattatggg tattaatgac
    48301 tccaaaaaac tgtccctaat taaaagagaa gaattgttcg aaataatcaa caagaaggct
    48361 ttgtccatag ggattggaat tgtcgatcct caaacgattg accgtatcaa tatattacag
    48421 gccacttttg aagccatgaa aaaggcttta gatgctttaa gtcttaagcc agaacttgtt
    48481 ttggttgacg gtaatcaaaa tatacccgat atttctgttc ctcaggaaga tattattaag
    48541 ggtgaccaaa aaagcatatc tgttgctgca gcagctattg ttgcaaaggt aacgcgtgac
    48601 aggataatgg ataaatttca tgagctttac agtgaatatg gctttaataa gcataaaggt
    48661 tatgggacta aggaacatat cgaagcaatc aaaaaatatg gtttatgtcc aattcataga
    48721 gaaagtttta ccagaaatct ttattgaggt gatggtatga agattaaccg ctatcagcag
    48781 ctggcttcta atagtcaaaa ggtgtatgct gctaatgaaa aaagtttatt aaggttaaaa
    48841 gctggggata tttttaaggg gcagattata gatataaaac aagggaagtt gtctttactt
    48901 ttggaaacag gggctactgt agaggcaaaa cttcaaagca atgtatatga gttaaaaatt
    48961 ggtcaaagct tattctttag ggtacaatcc ttaacaaatg aacaaatctt tatagaaatt
    49021 cttccggatc agtggagcga ttccaagtcc aatattattc tggaagcttt agaagcagct
    49081 aaccttttac ctacggatga aaattacaaa atggtagaaa agcttttaga aaagcagttg
    49141 cctgttgata aggataatct tcatgccatt aaccagttta ttaagtccaa taaaaacgtt
    49201 accctggata aaatcatatt tttgatggat aaacaaattc ctgtcaatga gaaaaatatc
    49261 agtcaattgg atggttatat tgaacaacat attcatttac ataagcaaat tgaaaatctt
    49321 ctggataaga tcataaagct cagtcatagt cctatagctg aaaaaattct ggattgtttt
    49381 cttgagactg gagataatgc tttaacggat aaagacatca atccggaaga aatcataaat
    49441 aaggccgact tattggataa agaagctcaa agggtcaggg caggagagac agaattaaaa
    49501 aatcaactga cagagataag aaaagaagtt gaattattaa cttttatgaa agataatcag
    49561 cacataatag aaacttttat gaatcagcat gaatttttta cagaagaaga gcttttttca
    49621 tttttgatga gtgagttccc aaccagtgaa ataaaagccc ttatgagtaa tgacaatcat
    49681 ttctttatgt tctttgctga aaatatgaag tacttactgg aaaataaaac agataatcaa
    49741 tatcttgaaa aagtattaaa caataaaatc aggacatttt tgaacgttct taaggaagag
    49801 ctttacctgc ctttggaaaa gttaaaaaat aaagaagagc tcaaagagcg tttcaataag
    49861 ttatataaat atatgaaccg gctagaaaac ctgttaacgg gacaggattc tgcacaagta
    49921 aaagatttgt acaaaacgtg tcaggaaata aaaaacaacc tggaatttat gaatcagttc
    49981 agcaaatttg atgcttttat acaaattcct ataaaattaa atcatcatca cagtgaggct
    50041 cagttatatg tctttcgtaa aaagaagact gcaaaaaaat ccagccattc actttccgta
    50101 cttattgctt tagatttagc ttatatgggt catacggaag tttttgtaca aaaaaatgat
    50161 aaggatttat atttgcaatt taaattagaa aatgatacat atataaagat tatgaaagaa
    50221 tatattcata ggcttcttga tgttttaagt tataagagct ataaaataag atctttaaac
    50281 tttcataaac tggaagattc atttaatctg ttaaagactg aagaaaatga ttataatata
    50341 aacgaagaac ccagaagatt ttcctttgat atgagggtat aaataatggt agcaggtggt
    50401 taaatggaaa ataataaaag aataaaaaag gcggcagccc tacattatga aaaaggaagc
    50461 accgctccaa aagttattgc aaaagggaag ggtgttattg cggaaaagat cattgaaaaa
    50521 ggtctggaag aagaaatccc tgtttatgaa gatgaaaagc tggctaatgt cctgacggag
    50581 ttggagatag gagagcttat tccagttgaa ttgtatgaaa tcgttgcaga aatacttgtt
    50641 tttgttgggg atatcgaccg tttacaggag aagattaaaa atggttaata aaagattaat
    50701 tggagactgg ggcgaagaca ttgcatcaga ttttttagaa aaagaaggta taaaaattat
    50761 tgagaaaaat ttcagatgta aacttgggga aatagatatt attgctgaag aagatgacta
    50821 tttacttttt attgaagtaa agtacagaaa agacctttcc catggctatc caagagaagc
    50881 ggttactttt cataagcaga ggaccatagc caaagtggct ttatggtatt taaaaaaata
    50941 taatttatgg catcgctcct gccgctttga tgttctggag atattaggtg aaaaaccaga
    51001 tttaaaaata tgcttaataa gaaatgcttt tgttgtataa tttttattat ttttaaaaaa
    51061 ggaagaatca gaatgtatca taacttatcc aatcaattat cttttcataa taaaaatgat
    51121 ttatattata ttacctttcc tgcttttgat gccagtcaac aggtcaggca ttgtttcagt
    51181 acccgtttgg gagggcttag ccagggacca tatcaatcat taaatttggg tcttggcaga
    51241 ggggatgatg atgaaaaagt ttatggtaat tataaattac tttgtgaggc tataggcatt
    51301 aatcatgaaa agctaatttt taccaatcag attcatgaca atcatattaa aattatccgg
    51361 aaagaggatt tggaaaaata taatatgaaa aactgggtca aaggtacgga cggcctggtt
    51421 accaatattc ctaatgttcc tttagtgact ctatatgccg attgtgtgcc tctttttttt
    51481 ctggacacaa agaaaaaagt tattggtctt gcccatgcgg gttggcgggg aacggttaaa
    51541 aaaatcgctt taagaatgat agaaattttt atcaacgaat ttaattccaa tcctaaagat
    51601 attttagcgg caattggtcc ttccattggg aaatgctgct ttgaagttga tttttctgtg
    51661 gcagatgcct ttcaaaaagc ttttaaaaac acccataaga taatttttcc tatagaaaat
    51721 gaaaaattta tgattgattt atgggaagcc aataaagaag ctttgacaga aggaggattg
    51781 cccctttcta acattactgt gacagattta tgcacaaagt gtcatcatga tatcttcttt
    51841 tcataccgcg cagataaggg gcaaacaggc agattggcag cagtaatgga attaatttga
    51901 taaatttttt attttggata taataaaaac agattagata atacaggagt tgttttaaat
    51961 ttgaaaaact ataaagaaca taaaataaaa gaagtccttc ctggcagtat tgctgaagaa
    52021 ttgggaatag aagcaggcga tttgcttatt gctataaatg ggcaagaaat agaagatgtt
    52081 tttgattatc gctatttgat ttcagatgaa tttgtgactt tgaccatttg caaaaaagac
    52141 atggaatggt gggaatatga aatagaaaag gatgttcatg aagacattgg tttagttttt
    52201 gaaaatgctt taatggatca tttgaaatct tgcagaaaca aatgtgtttt ttgttttatt
    52261 gaccaactgc cgccttctat gagaaaaagc gtctatttta aagacgatga ctggagaatg
    52321 tcttttttaa atggaaatta tatcacttta accaatatga aagaaaagga ctttgaaaga
    52381 ctgatttatt atcatttatc gcctgttaat atttcagttc atgcaacaga tcctgaagta
    52441 aggataaaaa tgttaaataa ccgttttgca ggagatgtat tgggaaaaat taaaagaatc
    52501 gtggatgcag gcatagaagt caactgtcag attgttttgt gtaaaggttt aaacgacgga
    52561 cttgtactta aaaaaacttt agatgactta gaaaagtttt tcccgggaat aaaaagcatc
    52621 tctattgttc ccgtaggcat ttctaagtac cgggaaaatc tttatccttt aatacccttt
    52681 gaaaaagatg atgcaaaaga gattatccgc atggtacatc atcttcagga tttctataaa
    52741 gaaaaatacg gcagttatat cttatattgt gcggatgagt tttatataac agctggagaa
    52801 gaaatcccac cagtccatcg atatgaagac tttcctcaaa ttgaaaatgg ggtaggtatg
    52861 ttggctttaa tggaggaaga atttaatgct gcttttttgg atttgccgga tgagatttct
    52921 ggaaagatcg atctgtctgt tgcaacaggt atagcagcct ttccatttat taaaaaatta
    52981 gtgaataagt tattagataa gtattccaat cttaccatcc gtgtttatcc cattaagaat
    53041 gacttttttg gggacaaaat tacagtgtct ggtcttatga ccggccagga tattatcaag
    53101 cagttaaatg gcaaagaatt aggcagcaaa ctattactgc ctgaaaatgc tttccgtaat
    53161 aatgacacga tcttcttaga tgatgtctat ttggatgatt tagaaaagga atttaggatt
    53221 ccagttgaaa ttgtaggcgt ttctggtaaa gattttattc atgcaatttt gaatgcaaag
    53281 gagaatagag atgagtaaac ctattgttgc aattgtagga agacctaatg tgggaaaatc
    53341 tactctcttt aaccgtttag caggtgaaag aatagccatc gtcgaagata cgcccggaat
    53401 aacaagggat agaatttatg ctgacaccga atggcttaat cataaattta ccatcattga
    53461 tactggagga attgaacctg attctgacga tattatttta aaacaaatga aaagacaagc
    53521 agaaatggct attgaaatgg ctgatgtcat tatctttctt accgatgcca aacaaggact
    53581 gacagacgca gaccatgaag tggctaatat gctcaggaaa tctaaaaagc cagtcgtttt
    53641 ggcagtgaat aaggtggatc actggggaaa agatcaaatg gaagtttatg aattttataa
    53701 tttaggttta ggagatccta ttcccatatc tgcttctcaa ccattaggct taggggatat
    53761 gctggatgaa gttgtgaaat attttcctca gcaggaagtc agtgattacg atgaagatgt
    53821 gattaaagtg gctattattg gtaagcccaa tgtaggaaaa tcttctttaa ttaataaaat
    53881 cttaggagaa gaaagggtta ttgtcagtga tattcccggc accacaagag atgctgtgga
    53941 cgtaccagtg accattgggg aagataagtt tgtttttata gatacagctg gcttaagaag
    54001 gaaaaataaa attaaagaaa atattgaaag atacagtatt tttaggaccg ttgctgctat
    54061 tgaaaaagca gatgtggctg tcttactgat tgatgcagaa gcaggggtaa ccgaacaaga
    54121 taccaaaatt gcaggaatcg cccatgaaag aggtaaagct tctatcattg cagtgaataa
    54181 atgggataag attgaaaaag acgacaaaac catgaataaa tacttaaaag atattgaagt
    54241 taaattcagt tatatgtctt atgccccaaa agtctttatt tctgctctta caggtcagag
    54301 aatcaataaa ctttttgaaa tgataaaagt tgtgtctcaa aatcatgctt taagaattga
    54361 gaccggggta ctcaatgagc ttttatatga agctatggca atgaatgagc ccccatccga
    54421 taaggggaag agattaaaaa tatactatat gacacaggta agtgtgaagc ctccaacctt
    54481 tgtgcttttt gtcaatgata aagaactaat gcatttttcc tatgaaagat atatcgaaaa
    54541 ccaaataaga gaagcctttg gttttaaagg aacgcctctg cattttatca taagagaaaa
    54601 gcagggaagg gaataagttt atgtttagac tggtatgtat aataataggt tattttttgg
    54661 gctgttttca aactgcgtat atcataggaa aaaaggttca aaaaattgac ataagaaatt
    54721 atggcagcgg gaatgccgga acaacaaatg tgattagagt aatgggttgg aaagtaggca
    54781 ttataacctt tataggagat tttttaaaag cagtcattgc tgttttcata tgcaaattga
    54841 ttttccctga ttgtcctgaa gcgggtttat atgcaggtct gggaactatt attggtcata
    54901 attggcccgt ctttttaaaa tttaaaggtg gaaaaggtat cgctgctacc atgggaacat
    54961 tattggcctt tgattaccgg ctgggcttga tttgtattac aattatggct ttagttctta
    55021 ttataagtcg tattgtttct ttaggttcta ttattatgtc tatatctata ccaatttgtc
    55081 tttatctatt ctataatgat cttgaaataa cgtttttggg agtattgttt acaattagtg
    55141 caattctaag gcatagggat aatatccgac gcttgtttaa aggtcaggag acagaattag
    55201 gtcagcgaac caatgaaaaa tatcagtaaa aataatttta acggaggtag ttatggaaaa
    55261 gattgctgta atgggagcag gaagttgggg aacggcttta gccatattat tagcaaaaaa
    55321 agggcatcat gtgacgcttt ggtgttattt ggaaagtgaa aaagagcaat tggaatcggt
    55381 cagggagaat cttaattatt taccaggtgt acctattcca caaaatatta tcattaccaa
    55441 tgattataaa gaagctattg taaataatga aatcatcgtt attgccatac cttcaaaatt
    55501 tctccgctcg aatatggagc aattttctcc ttatttaact cccaggcacc ttgttgtcaa
    55561 tgtttctaaa ggtttggaaa atcatacttt gctcagaatg tcacaggtga tagaagagac
    55621 agccccccaa tgtcctgtag cagtcttaat gggtccaagt catgcagaag aggtggcaag
    55681 agatattcct acagcttgtg tctcttcttc cagatctcaa aaaatagctg aaagagttca
    55741 ggatttattt atgacgccta cttttagagt ctacaccaat ccggatttaa ttggtgtgga
    55801 attaggtggt gccattaaaa atgtgattgc attagcagca ggtgttgtgg acggtttagg
    55861 ttatggggat aatacaaagg cagcgcttat gacgagaggt atggttgaaa taaccaggtt
    55921 gggtctggct atgggagcag atattcaaac ctttaatggt ttaactggga ttggtgattt
    55981 aattgtgact tgtacaagta tgcatagccg taaccgtcgg gcaggtattt tgattggaaa
    56041 aggaaaaagc ttagatgaag ctttaaatga ggtacaaatg gtggttgaag gtgtacatag
    56101 tgcaaaagct gtttatgaat tatccagaaa atataatgta gagatgccga ttataagaga
    56161 aatcaatcag gtcctttttg agggtaaatc ccccagggaa gcggtcatgg atttaatgat
    56221 gcgggaaaaa agacctgaac atcagacgga agaaattcat ttagcccata aagtttcgtg
    56281 gaaagacgag tagaccactc gtctttttat tttttataaa agttttaatt ggtataattc
    56341 cattttttag tcatatattt taaatgtacc catacatttt atattgatcg gggaaattat
    56401 cttaaggaaa tggaggatag tatggataat ttcaatattt atcaggatat tgctgacagg
    56461 acaaatggag atatttacat aggagttgtg ggacctgtac gtactggaaa atctacgttt
    56521 atcaagcgtt tcatggattt actggttatt cccaatattg aaaacagcta taataaagaa
    56581 agggctaaag atgaattacc tcaaagtgct gcaggtaaaa ccattatgac aacagaacct
    56641 aaatttgttc ccaatgaatc tgtttcgatt tctttaaacg atgaaattga gctcaatgtt
    56701 agaatgattg actgtgtagg ttacatggtg gaaggtgcca cgggtcattt ggaaggggaa
    56761 agcccaagga tggtcaatac tccctggttt gactatgaaa taccttttac agaagcagct
    56821 gagattggca ccagaaaagt aatcagtgaa cattctacca ttggcattgt ggtaaccacg
    56881 gatggaacta ttacagatat tccaaggaaa gattatgaag aagcagaaga aagggttata
    56941 agagaattaa aagaacttaa caaacctttt gtagttttgt taaattcggc aaaaccttat
    57001 gatgctcaaa cacaagaatt aaatgaagaa ttatctcaaa gatatgatgt accagtgatt
    57061 acatgtaatt gtgctcaatt aaaaatggat gacattaacc ggattatgga aaaagtttta
    57121 tatgaatttc ctgtgcaaga aattcaattt atgttgccaa aatgggttga aacgttagac
    57181 agggatcatt ggatgaaaaa aagaattata gaagcagtta aagatacgat ttatcctgtt
    57241 tatagtttta gagatttaaa agacgccatt tcttcttttg aagattatga ttttgttaaa
    57301 aaagtttttc aggaaaaaat taatcttgga aaaggtcttg taaaaattga tattaccgta
    57361 tcagatgaac tcttttatag ggtgttgtca gagtcaacag gtatggagat tgaaggagaa
    57421 catcagttaa tttctctcat tgtaaaactg gctgcaatca agaaagagta tgataaagtt
    57481 gctgatgcac ttaaggaagt aaggcaaaag ggttatggag ttgtgatgcc tttattagat
    57541 gagttgaaac ttgaagaacc tgaaattgta aagcaaggga accgatttgg tgtcagactt
    57601 cgtgccagcg ctccttcgat tcatttaatc cgggcagata tcgaaacaga agtatcgcca
    57661 atcgt
//
LOCUS       DAOSUD010000004        53825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_435_length_53825_cov_23.704873, whole genome shotgun sequence.
ACCESSION   DAOSUD010000004 DAOSUD010000000
VERSION     DAOSUD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 53825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53825
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_53825_cov_23.704873"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLG49_01205"
     CDS             <1..205
                     /locus_tag="PLG49_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ15958.1"
                     /translation="RLKLYEIEERLDKNYFVRISNSEIINLRKVKSFDLSFTGTICVK
                     LSNDTVTYVSRRRVGKVKKILGI"
     gene            215..661
                     /locus_tag="PLG49_01210"
     CDS             215..661
                     /locus_tag="PLG49_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HOQ15959.1"
                     /translation="MKKQVLLRSLLGIPMGIAVGYIITIIISVGWGGGHYYPCVPALV
                     NRMNSEICAVILQAVLCGLLGSVFGASSVIWEIENWSIVKQTGIYFLIIALVLMPIAY
                     LANWMKHSFFGFMTYFGFFVLIFILIWLIQYLFLRKTINKINGRIK"
     gene            927..1334
                     /locus_tag="PLG49_01215"
     CDS             927..1334
                     /locus_tag="PLG49_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15960.1"
                     /translation="MILSFSEEVDLAGNVINLRLKNFGHPEFNEKGEKFFNFQAEGEW
                     IFEIPLDYPAYKADSAIDRVLEVKGNPSTIMNLSLTPIRLNVEFLFPYTEDPDGKYSC
                     LFDDFENCYLITEDGEKLYVIKAFHLQRSGMMN"
     gene            1537..1659
                     /locus_tag="PLG49_01220"
     CDS             1537..1659
                     /locus_tag="PLG49_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15961.1"
                     /translation="MAKELIMENDSAVLWYHPESKIVHHQIKKYVYGKNCKNYY"
     gene            1674..1940
                     /locus_tag="PLG49_01225"
     CDS             1674..1940
                     /locus_tag="PLG49_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15962.1"
                     /translation="MKEKGAIKWLSDDRNSNAIRKADAEWGASVWFPLTVQYGWKYWA
                     IVQPEKLIGQLDMKRRIEQYAEKGITAKMFSDLDEALEWLEAQK"
     gene            complement(2167..2538)
                     /locus_tag="PLG49_01230"
     CDS             complement(2167..2538)
                     /locus_tag="PLG49_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOQ15963.1"
                     /translation="MEKLSFVMENYLEAIYELSKDNTGVRVSDISERLGVTKASVNNA
                     MATLAEKGLVINERYKEVYLTPAGLERAKLTANKHCIIRKFLTEVLKVDEEIADEDAC
                     AIEHVISWESIHAMQEFMEKN"
     gene            3012..3188
                     /locus_tag="PLG49_01235"
     CDS             3012..3188
                     /locus_tag="PLG49_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15964.1"
                     /translation="MIRAACMEDFKILCDLDEYISAKALEKKIKDGEVFVYEEEGKII
                     GWLRYNFFGMSIPL"
     gene            3185..3415
                     /locus_tag="PLG49_01240"
     CDS             3185..3415
                     /locus_tag="PLG49_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ15965.1"
                     /translation="MNMLYIIDGYRGKGIGKKLVSYWEEKMRQEGYHYLMTSTLANEE
                     AQHFYRKLGYKDIGGFLMPNEPLEIMLIKVLS"
     gene            4366..5073
                     /locus_tag="PLG49_01245"
     CDS             4366..5073
                     /locus_tag="PLG49_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15966.1"
                     /translation="MNEKNKKFIKITGITAIILIITGIILFWLLDLGSRLIRHPLNFQ
                     DMSKIKLEKPVNSVEEGYPYALKRAREWREDVVLTEVEIISSAIGTIDEIDDIEDRNE
                     IIRYTFEFEYMNEKQPSGFILVYLNVKTNCIDMVSAYHDDNNLFSPKDELDPEAISDK
                     IGKVYDAALKAIGKENILGSAGLYFSAKINSNFAVVSVYREIDNEEGNFENYEITIDM
                     STFEVTEMEIKEARREE"
     gene            5086..5592
                     /locus_tag="PLG49_01250"
     CDS             5086..5592
                     /locus_tag="PLG49_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15967.1"
                     /translation="MIKKGMMFIILMSVFVLVICSCAFTGDEKSETKNVQEQVNDADA
                     EFRTDIEPISKTFPNLGEVLECYWKADFFGGEASFRVPGPNPYWIQGFAKISEDDINK
                     FKEEYDWTLVEEGKKPDLETEILNLDEEDFEWYYSEEFNKYVKPAKYSGVFYIDFKNN
                     IIYFYVCV"
     gene            5962..6102
                     /locus_tag="PLG49_01255"
     CDS             5962..6102
                     /locus_tag="PLG49_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15968.1"
                     /translation="MKKYEKAMERIDQAFALEESAGDEHIIQIDGGVFIEPYLSHDDF
                     LV"
     gene            complement(6142..7275)
                     /locus_tag="PLG49_01260"
     CDS             complement(6142..7275)
                     /locus_tag="PLG49_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOQ15969.1"
                     /translation="MTIKKIGLFFLINTFILLTACGKNETQETTTELPPPVDRYIEAF
                     GIIKAGKIKNINLDFPAAIKKIHVSNGQKVNLNDPLITLDLSNYNKQLQDKENEYRIA
                     QLELQRIQAGETPSSQKESQRIKNELANKRKALSSNSDPDIQKLMNDLDLAQKSYEDA
                     KRDYEKQQILYQQGAISESEFDKIKRNLEQSKNNVESLKLSVEALKNNKQKEISELER
                     SLDLTNASLNSSNIEVEIQKQRILSLENEIALLKEKMNKPFIKEDKIVSDLKNAVVYE
                     IGYTEGDNTDQSKKVLSLLDLDSLIIEANVSEEFIKDVKAGAPVDIILLADSSKEYKG
                     KVTKISNMAIKENGETVVPIEISVDNKDSFLRPNFNVDVKIYK"
     gene            complement(7272..7961)
                     /locus_tag="PLG49_01265"
     CDS             complement(7272..7961)
                     /locus_tag="PLG49_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ15970.1"
                     /translation="MENHIENVIETRNLCKTFQLGDTSVEVLKDINISIKKGEFVSIM
                     GPSGSGKSTLLYLIGGLDKPTSGSIKINGKELSVMNDKEESTMRRQEIGFVFQFYNLI
                     PNLNVEENIMLPILLDGKKIKNYKDKLNEILEIVGLEDRRKHTPRELSGGQQQRVAIA
                     RALINEPDIILADEPIGNLDSKTGTDIMILLKKINEEKNKTIVQVTHSTEAAEYGNRI
                     IKVKDGKVWNQ"
     gene            complement(7979..10471)
                     /locus_tag="PLG49_01270"
     CDS             complement(7979..10471)
                     /locus_tag="PLG49_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ15971.1"
                     /translation="MGIIIKFLIKNIKEKKLRTFLIIFSITLSTALFFASNALSTTLT
                     DMLIKEMRTQIGSAEIIIYSNELSPTPYVDPKPAENLGNKLEYVIGTIELTGYYKAST
                     DDILNINILGYDYGELQKMNPIHLISSQNLEPFTGRKIIISETFAEKYKLELGSPMDL
                     EIQGTKQRFRVSAIAASKGLLKENDRNIPVIIPRETIASLAKARGKSFLLYIKTAEGE
                     NIPEILNELSALYNRYTVREFFSEEEILQQLSMIRTPFLLITLLVLFMSIFIIYTSFK
                     VITMERLPVIGTFRSIGATKKMTSGVLLLESILYGIIGGVSGCVLGIGVLYGMAAVIA
                     NDPYMQIDVSIKFTFGQLRQALTLAIVLSSLSALLPVLKISKIPVKDIVLNNIEKKYK
                     KKKWKLYFGIGFLSGALILPPIIPHSIAFIINAILMIAVFVALIYLIPYLSKVFICLF
                     EKIYIFFFGNIGVLGAKNLRENKSILNNISLLTIGIASIIMINSVNYSMNIEVLDAYK
                     ELNYDIQFYIYGMDRRFEQVIKSTPGIAASYGIYGSYDNILVKESGQSLGYVQGIDVS
                     RFLDFYDYDIEPELLKKLDTGRYILLTYAQGKKHGVNIGDTLTLNMKKGERKYTVLGF
                     FNALTLMYNGQYALIAQKYYKNDMDSPFYDELALKTSKDPEEMVEILNKKFKRNYPYI
                     NSVNQLRKDIMQSNSQMMGLLQGFSFMTVIIGIFGVLNNLLISFLERKRQLAIMRSIG
                     MEKKQNTKMLFIESLTGGLIGSITGVIGGIFVNYVIEHLLEAMNLPMNIHLSPSLFVS
                     SFIIGTMVMVMASISPNLKSSKYNIIEAIKYE"
     gene            10754..10933
                     /locus_tag="PLG49_01275"
     CDS             10754..10933
                     /locus_tag="PLG49_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15972.1"
                     /translation="MVQMDELKVKLREYGIMCYETIKKDGFPTIYMPDNDLESFLDFV
                     MNNQIKNFFKSSDIM"
     gene            11173..11514
                     /locus_tag="PLG49_01280"
     CDS             11173..11514
                     /locus_tag="PLG49_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15973.1"
                     /translation="MLPRHQKLAEIINKQVHVIERIYQEQRLKEEQEKKASERLLEEF
                     KNFVLNDPDFKTCTFPGARRDYAREVFTTRKDTAKYRHLFYAEDDVDYIDNQRAYYFI
                     ESIWREYRSPN"
     gene            11763..11936
                     /locus_tag="PLG49_01285"
     CDS             11763..11936
                     /locus_tag="PLG49_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15974.1"
                     /translation="MGSLFNIISIITYWGTARAVLAFIFFLIDTGGFPWLIYSMAKEN
                     WWREEKDKRERKK"
     gene            12112..13530
                     /locus_tag="PLG49_01290"
     CDS             12112..13530
                     /locus_tag="PLG49_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOQ15975.1"
                     /translation="MISLHPRLLVLKCSIINTLIKGEPKIDKKNVIIIGAAGRDFHNF
                     NTYFRNNPNYNVVAFTAAQIPDIDNRKYPAELAGDLYPQGIPIYSQDRLPELIKELDV
                     DECVFAYSDVPYQTVMGLSAIVNAAGADFKLLGPKHTALKSKKPVIAVCAVRTGSGKS
                     QTSRKVIELLMAKGLKVVAVRHPMPYGDLNAQRVQRFATIEDLKKHKCTIEEMEEYEP
                     HITRGNIIYAGVDYGDILRSAENDPDGCDVILWDGGNNDFPFYQPDLTITIVDPHRPG
                     HELSYYPGEVCLRIADVAVINKIDSASPEAIATVEANIKKVNPSAKIIKAESKIIVDN
                     PDVIKGKRVLAVEDGPTLTHGEMKLGAGTVAAERFGAKSLVDPRPYTVGKLTETFKIY
                     PNIGTLLPAMGYGEQQLKDLETTINNTDCDSVIIGTPIDLSRVININKPYTRVYYDLD
                     EVSSPNLNDIIDEFVKKFNLEK"
     gene            13794..14408
                     /gene="wrbA"
                     /locus_tag="PLG49_01295"
     CDS             13794..14408
                     /gene="wrbA"
                     /locus_tag="PLG49_01295"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOQ15976.1"
                     /translation="MTNVKLAIIYYSMTGTNYQLARWAEEGAKEEGAEVRLLKVPEIA
                     PQSVIDSNPAWKTHLEATKDVPVATTEDMDWADAIIFSAPTRFGGIASQMKQFLDTTG
                     GFWAQGKTVNKVVSAMTSAGNVHGGQEATILSLYTSMYHWGAIVVTPGYTDASIYEAG
                     GNPYGTSVAIDNSGKMKDDVEKAVKYQAKRVIKVAAALKKGWGN"
     gene            complement(14467..15810)
                     /locus_tag="PLG49_01300"
     CDS             complement(14467..15810)
                     /locus_tag="PLG49_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ15977.1"
                     /translation="MDDNPFIWLLLLQLVFIFFNAMFACAEIAVITMNDNKLAKLAAS
                     GDKRALRLSKLTEQPSAFLSTIQVGITLVNLLSSAVATENFSDRLVNFFIDLNLNIPK
                     AIINAFSVAIITVLLTYFTVLFGELIPKRLAMKNAEKIALGMSSLIYGLSRLFTPAVW
                     LFTVSTNSILRLFGIDPNSETEENAEEEILMLLDAGKQKGTILPDEQNMIQNIFEFDD
                     ISAAEIMTHRTEVALLWLEESDAQWEKTINESRHSVYPICSDSPDNIVGILHTKDYFR
                     LKNKNRSEVMKKAVQKPYFVPETVRADVLFRNMKKSRNHFAVVVDEYGGMSGIITMND
                     LLEELVGDLEDDISIPEEAPPIERIDSKTWRIQGTAPLDEVSKELGVMLPEEEYETFA
                     GLVFGLLGTVPSDGSTPELEEYGLIIKVTEIKERRLESALVCLAEAEPPTSTADN"
     gene            16372..16647
                     /locus_tag="PLG49_01305"
     CDS             16372..16647
                     /locus_tag="PLG49_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ15978.1"
                     /translation="MVKQQEYKVIRNTVRVETEEKGQSIYDAPDHMKYIITSAPQLGI
                     LKSLGIVKNASVYKEMTHRFGGPVLLKVESSKIAIGKDIAEKILVRR"
     gene            16652..18436
                     /locus_tag="PLG49_01310"
     CDS             16652..18436
                     /locus_tag="PLG49_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOQ15979.1"
                     /translation="MGECHEVISDENIYEKPDKILLMGNPNVGKSVFFSHLTGIHVVS
                     SNYAGTTVTYTEGTLQLGDKTYTLIDVPGVYSLEATSEAEAVAVSFLNSNPVAILCVL
                     DATNLERNIKLGLELKKYNVPIVYALNLLDVAKRHGVEINVGLLAKELEAPVIPTVAV
                     KDEGINEVKEALGKLLKHSFLNCSHCTSCPECPGIKKDEMEFWNRAREITAKVRKNVD
                     SKLSFLDRLGNSMLKPWPGIPIALLVILLSLGVIVGGGKILQEGLLLPWVNKIIVPFF
                     KSIFASFIPEGILLNILIGEYGIFVIGFEWIITLILPYVFLFYVVFSFLEDSGILPRL
                     SVLFDNIMRKMGVQGGSMITILMGYGCAVPAIISSRTATTRKERLIISTVVCFAVPCI
                     SQTGALISLFAAFSPLLLILMILLSLVIMMIVSGVLSRMLKGTVDPMVIEIPNLLMPN
                     AHAYGKKLMIRMKYFLLEAEVPMLISILIAAVLKETGLLAYIAAYLEPIVSGWLGLPR
                     EAVIALILGIVRREMAVTPLLAIEGLTALQAFVGGTVALLYLPCLSVFGVLTKEFGPK
                     VAVAIACSTVVTAFTVAGLINQIAHIFI"
     gene            18611..18913
                     /locus_tag="PLG49_01315"
     CDS             18611..18913
                     /locus_tag="PLG49_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HOQ15980.1"
                     /translation="MSKELRFNELKEEHLKILGQYVPVVAKVHGESHPEFHEVHKIFE
                     KINEKIKAGDLEKPNLDEEFASLRKVTNNYTVPADVCETYEKVYRLLAELDQAYQA"
     gene            18931..20781
                     /locus_tag="PLG49_01320"
     CDS             18931..20781
                     /locus_tag="PLG49_01320"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ15981.1"
                     /translation="MKKVILKQKNQIAFISGILIALAFISKLLFKNELVFNGLLIIAA
                     FLGVSPIAIQGFQALKVKLVSIDILVTIAVLGAFVIKNYEEAAIVTFLFLFGAYLEQR
                     TLNQTRSAIKELTEMAPDTALKQTEAGEFEEVDLDEVDAGDILLVKTGAKIPVDGRVL
                     TGEGHVNEAMITGESVPVSKKKGFKVYAGTILENGTLQIVADRVGEDTTFGRIIELVE
                     EAQDSKSQVERFIDKFSKYYTPAVLLFAFVVWIFSKDIDLSITILVLGCPGALVIGVP
                     VSNVAGIGNGARKGVLLKGSEVIHSFSKVDTIVFDKTGTLTIGNPKLADIKFYTKDET
                     EVLNYLASVEKESDHPLAKAIIEYLEGARFYTVEQTNVVKGGGIIAYVNGRRIAVGNL
                     ELMRQENIPLDEHIIKDICQFEDKGNSLVLTAIDGQLKVLMGIRDKIRPDVKENLQKL
                     KTLGVKNLIMLSGDNQGTVDLVARELGLTKAYGNMLPEDKSAYIKKLQAKDQIVAFVG
                     DGVNDSPSLALADIGIAMGNGTDVAIETSDIVLMNSDFSRLVYALGLARATVWNMRQN
                     IIIAVGVVFVLLFSVFFSDWMNMSLGMLIHEGSILAVILNGMRLVRYKLR"
     gene            20846..21073
                     /locus_tag="PLG49_01325"
     CDS             20846..21073
                     /locus_tag="PLG49_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOQ15982.1"
                     /translation="MKKATIQLETLVCPSCSQKITNGIKALEGVDEESVTVSFNSSKV
                     KLNFDEEKLSIKEIEDAISVLGYEVIKSQVK"
     gene            21317..22261
                     /locus_tag="PLG49_01330"
     CDS             21317..22261
                     /locus_tag="PLG49_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15983.1"
                     /translation="MGVMGIAEIMDRAVNIFKKRIGYFALFSLISGGIFIVLYYLITT
                     PFSLLLLSFSEIWGLLLFIAVLFLILMIFAFSYAIGIMEIIRQDIFFNKLSVASAIAS
                     VFKNIPKTIAIVIINCILFFPLIAVYIFFLYYFDVVLSENIIIDLVTTDALYIIIFVI
                     LCLLGNIIFVAFMGFTLFCLFVVVEENCGIIDALNKALWLIKNEIFKILWSLTVIEMT
                     VFSIYLSLISFSLIISGIIIAICMSFFKLNELMTTLLVLPLSLLNWSLSQITFLIIGI
                     LAIIMACLLCYNQKFKKEGYDIIIRLTQLENRKRSVKQ"
     gene            22258..23874
                     /locus_tag="PLG49_01335"
     CDS             22258..23874
                     /locus_tag="PLG49_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15984.1"
                     /translation="MNLLIMKTPTKPVFDETVKKVLMKHGYIREEKEPLDISLPAFNL
                     DKNVMIVVMVMIIMLLVTVLIIQAKKSYKRKKQIKEIMGVKIEENTTPYSLKNKANVH
                     EERGEFRYAIRFNYIALLFLMHESNVLYLDETQTNKEIIQYLMKNGFNLVPQMSFLME
                     YFNHYWYGHKKCDMDSYQQWKNVLEMLWNEIQSGKNKTSKNTVFSTCLILAIVLFTTA
                     CSGSSGPYSVKDKGPFGLSVFYITLKELDYPVKRSLKPIENHGHNTVQIVADNNYLDI
                     NNEKIQEWVKEGGIIVFLKDSTSENILYGEIKEELIGEVYSYGKGYIIVNDVSVVING
                     MLTESTEEAYNLLKQIDQFSYEKIYFNEEHFYSKNETQNEDKTEEDNELPDLNTESLW
                     KYLPASVKMIIFQLILVMMAYFYFKGKRFGKIIPLYEEVERSENEYLYSVAYLYKKAK
                     CMDIILEIYYKSFIRKIPMAWHDDWLKYWEEEGLASYSQAKKIYHLMNNQERNLNRKE
                     LISYINTIEKLKKVLDKRRDLYWKTLKSDL"
     gene            23847..24812
                     /locus_tag="PLG49_01340"
     CDS             23847..24812
                     /locus_tag="PLG49_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ15985.1"
                     /translation="MENAEIRFIKYHIDRIKKELRKVIIEQNELLEYSLIALIAGGHV
                     LLEGVPGLAKTLMIRSLAKTLSVDFKRIQFTPDMMPADITGTKVFNMQTRTFELKKGP
                     IFTNFLLADEINRTPPKTQAGLLEAMAESSVSIDGEVMPLPNPYMVFATQNPLEYEGT
                     YPLPEALVDRFLMKIVIKYPSLQGEKEILKRHHEGFSSDDLDRANIEAVCKKEEILAC
                     RQLVSKVKVDESLMEYIVQIIAETRNNPVIEVGSSPRGGIALLNCSKACAAYYGRDYV
                     IPEDIKNMAIPTLRHRIILKPEVELEGITADQVLMNILSKIKVPR"
     gene            24828..26150
                     /locus_tag="PLG49_01345"
     CDS             24828..26150
                     /locus_tag="PLG49_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ15986.1"
                     /translation="MNITKRYIYLLFLGAVILTVFSFLNSWLISFTIFLIYNGVCFLL
                     LMIDYFTSPGKEVFEISRWGNNHLSIYEKEEIAFMVYYKGYKKTRVEMKDEIPDFHFK
                     TDKKVIKAELLPHSKTKLTYLVTPTKRGAFLFGKLHVRYEGNWKLCKKAFIVDLSLEY
                     KVYPNMKNLRKYRMTGLNNHQWKQGQKHLKMLGKGTSFESLREYVPGDEYRKINWKAT
                     ARGDKPIINQYEPEKDQHVYFFIDTGRPMSYSIRGYNKLDIVVNTALILSDVVNQNGD
                     LSGLLLFNTEVDNMIAPGKGIEHRNKILETLYHIQHTNYTSNYEEAFYYFKRRERHRS
                     IIFFFTDFDSFEEAEMMFKVLSVITRNNLVVIALMKDEQVEQIASSDIKSELDLYNKG
                     VALELMDERKKIIRLLNQKGVFCLESTPEKMELATVNKYIQIKNKLIF"
     gene            complement(26140..27090)
                     /locus_tag="PLG49_01350"
     CDS             complement(26140..27090)
                     /locus_tag="PLG49_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOQ15987.1"
                     /translation="MKEEQFIKSKSATWKTLECLSAHIDKKGITSLNSKEIKEFLHTY
                     KLTSHHLAYARTHYPNSNTVTYLNSLIGKCHNHVYLVRKSSFKESFHSFFYDFPNALK
                     ELKAYVLISFGIFFFGFLLSLIMVFYDDSLARFFLNETMIEAISDGQRLGSGQWDYPL
                     ISSKIMTNNISVAFKAFAYGISFGIGTIYILFTNGALLGALTGLVYLYNDPVRFWSLI
                     LPHGVIELTAIFISGAAGIMIGKSLLIPGEYSRKHALIAQAKRSVSLLIGVVVLLVIA
                     AIIEGFFTPLEIDEDIKLIFAAFAGILLILYFSLPYLIKK"
     gene            27168..27860
                     /locus_tag="PLG49_01355"
     CDS             27168..27860
                     /locus_tag="PLG49_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ15988.1"
                     /translation="MKIKIITPENIEVEYSLANLGSRAAAFIIDSIIEGLIFSILLLI
                     LFCIYKINEPFWEAFYGWIWGIFTIVSFVIYQGYFIFSEMNMNGKTIGKRLMRIRTIR
                     RNGQSLTFKHSLIRNLFRTFIDVFGIGVVLMFLNKEHKRLGDMVASTIVVAEEDKTRP
                     VSLESLLKSNQGTYNYYLSQHEQDLLREYYSRRYYIEDSEELRKRMRLYFAEKFEREG
                     LLEEFKDFINNL"
     gene            28022..28663
                     /locus_tag="PLG49_01360"
     CDS             28022..28663
                     /locus_tag="PLG49_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ15989.1"
                     /translation="MRVVKTADERRNEILDAAEKLFIQKGFDATSTNDILEEVGIARG
                     TLYYHFESKEAILDALIERYNKKVLDKAKEIARDKSLSIIERLMGAVMALNIKQKSER
                     EIIEEIHKPQNALMHQKVQRLILTEVTPILAGIIEDGIREGLFNTPYPYETIEMLIAY
                     TNTVFDDEIVKMTEDEKKRRIAAFIFNLERLLAAPKGTFISIMKAFEDSEKNE"
     gene            28656..29948
                     /locus_tag="PLG49_01365"
     CDS             28656..29948
                     /locus_tag="PLG49_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ15990.1"
                     /translation="MNKEKVLFRKFMILWLGQFISAIGTGLTSFGLGVYVYELTKKAS
                     LVALISLFAFLPSILLGPLAGVLADRYDRRLLMIIGDSFSALGPLFILFSILFGKIEL
                     WQIFLGVLISSVFSSLLEPAFKATITDLLDENQYSKASGLNQLAGSAKYLISPLLAGM
                     LLVISDIELLLMIDIFTIVITVCTTIIVRKGIAVKVQEERHSFIADFKEGWNEISKKE
                     GVKVLVAVTSLMTFYVGIIQTLTTPLILAFSNSAVLGMIETLSALGMLFTSFVIGIIS
                     IKSGYLKMLSKSLFFAGICMFFFGLRENIFLICISGFVFFAMLPFANTSLDYLIRINI
                     NNFVQGRVWGLIGLISQSGYVIAYAVSGLLADYLFTPLFIEGGLFYNSLGKIIGTGTG
                     RGTGFLIMITGLLLSVTAIFLYHIKSIQKLEKRGEICI"
     gene            29939..32257
                     /locus_tag="PLG49_01370"
     CDS             29939..32257
                     /locus_tag="PLG49_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ15991.1"
                     /translation="MYLKIIRNDITKSKLITVITVLFISAAALLISLVAVLAVNLLGA
                     VDTLMTQGKAPHFLQMHSGDIDLKRLEQFARENDKVDEFQVLEFLNLDGEKIQFEDMT
                     LRDSVQDNGFTIQSKKFDYLIDLEGKVIQPVKGELYVPVSYMKENITKLGETVKICGK
                     EFIVAGFLRDSQMNSSLCLSKRFLISEEDYQEIKNDGNVEYLIEFRLKDLSDLAEFEN
                     EYIAAGLESNGPVITYPVFKIINGLSDGIMIALILLVSILVILIAFMCIHFTLLAKIE
                     EDYREIGVMKAIGLYVSDIKKFFLAKYILISAAGCLAGFFLSFFFREPLLYNIRLYMG
                     ESPKSHLSLYVAIIGVVFVFYTILTYVKKILNRFKRISPSDAIGFGVSKEKTKYVKYF
                     LLSRSKLPEINLFLGINDVLSKKKLYLTMFMVFVISNFIMIVPQNLHQTVSSKNFIKY
                     MGIGASDIRIDIQQLEDIHAKSMEIIKRIKEDQTIKKYAVFTTKAYRLIHSEGQEEIL
                     KVELGDHTLFPIEYAKGHPPMTENEIALSVIKAEELNKNLGDSISLLIDGNEKSFEVC
                     GIYSDVTNGGKTAKANFRDDNTDTMWVIIQLELSDKTLLSEKTQEYAQAFPFAKISGI
                     DAYIGQTFGQLIHSVRIASLVSVGVAAFLSILITLLFMKMLITKDRYAIALMKSLGFN
                     NTDIKFQYLFRSLFVLFISIIIGTILANTLGELMAGAALSYFGASYFKFEINYLKAYL
                     LYPLLLMCCVITGTIIGSLRAGEIEISESIKE"
     gene            32264..33022
                     /locus_tag="PLG49_01375"
     CDS             32264..33022
                     /locus_tag="PLG49_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ15992.1"
                     /translation="MKKIIVAEKVVKSFGADGQCRKVLDNVSLTVYEGQFISVMGPSG
                     SGKSTLMFCLSGMDKIDSGRVIFDGKVLSDLKEKEFADIRRNEMGFVFQQPTLLKNLN
                     ILDNIILSSMKDLNKNKEQIIKKAQKLMKKLGISELEKRDITQASGGQLQRAGICRAL
                     MNNPKIIFADEPTGALNSKSAEEIMDIFDNINKEGTTIMLVTHDAKVAARTERILFIK
                     DGKIVSELKLPKYEGKNMEERLEKVTDAMLELGI"
     gene            33198..33989
                     /locus_tag="PLG49_01380"
     CDS             33198..33989
                     /locus_tag="PLG49_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ15993.1"
                     /translation="MIEANELCKIFTRYVKDEEVTNKRKKIKTKKEDFFAVDHISFKA
                     RQGEIYGILGPNGAGKTTLLRMLGGILTPTSGEIKLAGYDYNLDRKKVKRKIGYLSGN
                     TKLYGRITPRELFNIFGRLYEMSKNEIEEMTEKIIEIMDMKDFADNRIEHLSTGQMQR
                     TSIARCLLHSPEVYIFDEPTLGLDVISSHAIIEFMKSERERGKTVLYSTHYMEEAESL
                     CDRILMIHKGKLIAEGTPESLKKESHENNLRDVFIRLVKERGEWT"
     gene            33986..36019
                     /locus_tag="PLG49_01385"
     CDS             33986..36019
                     /locus_tag="PLG49_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOQ15994.1"
                     /translation="MRKNIVLSIFQKEMLDLLRDKKTLFMMIILPIIMYPVLFILFSF
                     LMIMGISSLQEKELPIAFNKSPDAAIIAKILGGKEKTGKLKIVEVEDYQKALEEREIT
                     AYIEIEEEDGQIQYKIYMNSSVEDSNESTGRIKDLLEEYKDELVKEKIEAEGLDVDLI
                     LEPIVYETVDLAKKTEKAGYLMGQILPVIFIIGILLGAVYPATDVIAGEKERGTLETL
                     LTLPVSNGELVMGKYLAVSLSAIISAILSVLSISLSLLFLMGNFKEAGAIDLEGINYN
                     ELIFPLIITLICVCLFAMVVSAISMCVCSFAKNYKEAQNYISPIMLILMIPSYVSVMP
                     NMELNGVNAAIPVVNISLLIKNVLSFNYNLAAISVVLVTNIAFVLLSIYFLSKIFKSE
                     DILFGDKREFSFLEKRSNIKKGSLPTLSDGLILYAIALLVLIYLGGFLQVKYQILGMA
                     LTQFMIIAFPLFFGYYIKTDFAKTFSIRVPRLKDFLGGFILWIGVSIASLLLAQFILY
                     LFPENMEVAEALSEFITGDYPFILSLLVFALLPAICEEALFRGFLMTAFKDKKAILRA
                     IILSSLLFGVMHIDFIRIIPTTILGLALGYAVYCTGSIFIPMLMHFLNNGIVLFSGYF
                     PEHIISKAYSCLEIDFSNLDFGHLISLIVLSIVFTIIGLVLLKKPKNEKVPGS"
     gene            36146..37069
                     /locus_tag="PLG49_01390"
     CDS             36146..37069
                     /locus_tag="PLG49_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ15995.1"
                     /translation="MNLQYLRYALEVEKTGSISKAAENLLMSQPNLSRAIKELEESLG
                     ITIFKRTSKGVSATYQGEEFLSHAKRILSQVDEVEAMYKNRKANKQRFLISVPRASYI
                     ACAFINFINKIDINQDTEIYYKETNSLRAINNILQADYKLGIIRYQTSFEQYFNSMLY
                     EHGLASREILSFNSVLLLSKEDPLARKDKIEPSDLRGYIELAYPDPYVPSLPLTDARK
                     AEHSKCVEKRIYIFERASKFDLLKNIKNSFMWVSPMPQELLDLHQLVQKPCKFNKKIY
                     KDVLIYRKDYYLSDLDQAFIDEIDKMKEKIK"
     gene            37262..39871
                     /gene="adhE"
                     /locus_tag="PLG49_01395"
                     /gene_synonym="adhC"
     CDS             37262..39871
                     /gene="adhE"
                     /locus_tag="PLG49_01395"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HOQ15996.1"
                     /translation="MDKNKTYTVVDSVEKLDQAIQKVRKAQEVFATYSQEQVDKIFFA
                     AASAANKERIRLAKMAVEETGMGVVEDKVIKNHYASEYIYNAYKDVKTCGIIEEDKAY
                     GIKKIAEPIGVVAAVIPTTNPTSTAIFKTLIALKTRNGIIISPHPRAKKCTVEAAKVV
                     LEAAVAAGAPEGIIDWIDVPSLEMTNYLMSEADIILATGGPGMVKAAYSSGKPAIGVG
                     AGNTPAIIDDSADILMAVNSIIHSKTFDNGMICASEQAVIVLNGVYNKVKKEFARRGC
                     YFLNKDEIDKVRKTIIINGALNAKIVGQTASTIAQLAGIEVPENTKILIGEVESVDIS
                     EEFAHEKLSPVLAMYKAKDFKDALDKAEQLIADGGYGHTSALYIDTVNEKEKLKEFEA
                     RMKTCRILINTPSSQGGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGVKHLLNIKTVA
                     ERRENMLWFRAPEKVYIKKGCLPVALEELKNVIGKKKAFIVTDTFLYQNGYTKVITDK
                     LDEMGIKHTTFYDVEPDPSLASAKEGAKQMAAFEPDCIIAVGGGSAMDAAKIMWVMYE
                     HPEVDFMDMAMRFMDIRKRVYTFPKMGEKAYFIAIPTTAGTGSEVTPFAVITDEETGI
                     KYPLADYELMPDMAIVDPDMMMNAPKGLTAASGIDALTHSLEAIASMLATDYTDSLAL
                     RAIKIIFEYLPRAYDNGPNDPVAREKMANAATMAGMAFANAFLGVCHSMAHKLGAFYK
                     LPHGVANALMINEVLRFNAEEVPVKMGTFPQYAYPKTIEKYAEVADYLNLGGKSNEEK
                     LENLIKAIEELKEKVGIKKTIKDYGIDEKVFLENLDEMTEQAFDDQCTGANPRYPLLS
                     EIKQMYLNAYYGR"
     gene            39885..40634
                     /locus_tag="PLG49_01400"
     CDS             39885..40634
                     /locus_tag="PLG49_01400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOQ15997.1"
                     /translation="MKLDTILAVRKSKTIYRDGDKVIKVFDKDFSKADILNEALNQAR
                     IEETGLNIPKIIEVTTVDGKWAIVSEYIEGKTLEVLMKENPDKFDVYLEMFVDLQIEV
                     HSKKSPLLNKLKDKMIRKINETDLDEHIRYELRTRLEGMPKHKKVCHGDFNPSNIIIK
                     PDGTPYILDWSHVTQGNASADVARTYLLFSLAGESDTAEKYLDLFCKKTNTSKRYVQG
                     WMPIVAASQLVKGKPEEREFLRRWATVVEYE"
     gene            40787..41317
                     /locus_tag="PLG49_01405"
     CDS             40787..41317
                     /locus_tag="PLG49_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ15998.1"
                     /translation="MKEKTKKIRSFRFKWYEFFLIYGLINYYFFTAINSSLFSLQREM
                     INCYSDNLEIDGTDFVPFFNFMSFSFLTGLWIIVAIIIIMISIISLLIFRFIYFRSVI
                     NQEEINKLLKYMKNTIILLIMVNIAASTILFYIEYHSFNYIIIHLLGFLGVYVTIPLF
                     TYLLIYVKLKKIHNIS"
     gene            41353..42273
                     /locus_tag="PLG49_01410"
     CDS             41353..42273
                     /locus_tag="PLG49_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ15999.1"
                     /translation="MYANNPSWKVLQNFLPEKNRITERITPEEKYIKIKDMEIHLDEY
                     NNNSDTSIIMFHGVGGNGRLLSFIAVPLFKAGYNVLCPDLPGYGYSRYNKLLTYSDWI
                     DTGAAIVERELAKSKKVFVMGLSAGGMLAYNVVCKNQEVQGLIVTNLLDNREEEVRIH
                     SAKNKLQAKYGIQLLNHFPGFLKTIKLPIRMVTNMNAIVNHKEVLKVILKDKLGAGNK
                     VSLNFLLSMMNSIPLIEPEDFKKVPVLMVHPGNDLWTPVRISDLFFNRIASSKYRVIL
                     KNAGHFPVEEEALVQMEREIVHFIRSLLQK"
     gene            42350..43465
                     /locus_tag="PLG49_01415"
     CDS             42350..43465
                     /locus_tag="PLG49_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16000.1"
                     /translation="MFKYKKFCLLPALLIFVFFFSACSKFIAGEKQNDKTEIPQKTNE
                     VITENKEKKKLTREEVIAIEKAFNERIFQLEKVEDSNEILNFKSKEELMDYVKEVAGE
                     ELATLFIENFYEEIDGKLYVIPKGTPPRLIYDSPFEINKIDDNTYEIVQDEENEMYGP
                     YRLIIEFKYIDGKWKMTDRKAEGRENANQDDSNFSKEERIDSETAKAIISDTANRLIY
                     ALSIKDVNTIAEYVHPEKGVRFTPYTFVSLEEDLVFSQEEIKNFFEDKNIYTWGYYDG
                     SGEKINLTPAEYYDSFVYSADFINAPEIGYNEVLSQGNMLENQFEVYHNPIIVEYYFP
                     GFNPEYEGLDWQSLRLVFEEYEGTWKLSGIIHNQWTI"
     gene            43570..44598
                     /locus_tag="PLG49_01420"
     CDS             43570..44598
                     /locus_tag="PLG49_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HOQ16001.1"
                     /translation="MKIRWYGKKITAGLLLTVLMVPTFSQGADVNLVSENIEIIPVRY
                     EYKHWAQPAVEILSQNYDIGQFFEGKHLNDFISAEDFQILIQKTIDKDYNGVPDALTR
                     EAVVYELVKIWAKESHQDLNTIGVIEMLIYSDTDRISPDYNHAITIAYMKNIAKGKGS
                     GIFDPKAKVSYGELAALINNTIAAIEKESSQQDIASEKFETRGSYDIKDDKVILDFEL
                     MNHYNNPKDLMFSSGQQFEISIIDEEGNEVYRYSDGKFFTMALVYKTINPGESLKWQD
                     VWDMTNKDGEKVKTGKYTARISILCQEINSDEAALEESVSALDLSSVLETEKEKKSEF
                     TTIIEIDL"
     gene            44907..46268
                     /locus_tag="PLG49_01425"
     CDS             44907..46268
                     /locus_tag="PLG49_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ16002.1"
                     /translation="MKLRYVMDMTEGNEVSLMIKFSLPMLIGNIFQQFYNMVDSIIVG
                     KFVNANALASIGVTSSISFLFFSLCLGLAVGVGIIIAQYFGARDDKNVKSAIANSIYV
                     IGSTSIFISILGIFLARPVLEFMKTPVEILDDAVLYLQIIAGGTLAVGAYNAMSFILR
                     ALGDSKTPLFFLIIASVINVILDLVFVLVFGWGVAGAAIATIISQIAAATGCIVFAVK
                     TEYYFRLKKEELKVNKNIIKETIKVGLPVAIQNSTIAFSLLALQNIVNRFGRVAVASY
                     TAVMRIEQLIQQPFNSLGAAMSTFSAQNMGANKIDRIKKGYHKMIFIIAAFALMAFSG
                     AQFGGRYIMRMFVNEEDVIALGAKGIRISSCFYFFLGMIYITRGLLNGAGDTVYAMIN
                     GFVEVIGRVGFSSILYFFPVFGVMSIWITTGLTWFITAGASVVRYIQGKWKTKSIVKV
                     NEQ"
     gene            46475..47167
                     /locus_tag="PLG49_01430"
     CDS             46475..47167
                     /locus_tag="PLG49_01430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOQ16003.1"
                     /translation="MKNRTEKTKNITWREMMDILIRNEVKEDYKQVEELTREAFWNLY
                     LPGCSEHYLVHVLRKHPDFIAELDFVAVLEGKIVGNIMFTQSRLINEEDDSIAVLTLG
                     PVSVLPEYQKKGIGSKLIQHAVKKAAEKGYKVIVLEGYPHNYCKHGFLGSKSLNVSDK
                     NGRYPYSLLVLELEKGLLKNHSWKYHPSDAYDIDENEANEFDKMFPPKKKAYQPSQEE
                     FNIACNAYIEDL"
     gene            47337..48350
                     /locus_tag="PLG49_01435"
     CDS             47337..48350
                     /locus_tag="PLG49_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16004.1"
                     /translation="MRRNYEQVCSNIKSLFYFGLFLLISIGLTACVQQGASDLTSDEV
                     VHSEIEVVEKTEMAKEKTEYLDKNKAEKSNEEINHFETNPEDFFNLQENIIRNVLEVN
                     KEYDDEVFELIVRGKYDLNKDGSLDSIEARLKYRDESFLRINNTEFLMTSDNPCDMYL
                     IDLVEDDSFIEIVVYDDGPSGDPVSTFYRYDGKNIYELGHFHTHIDTEDTIYSYYGNV
                     LTDGKGNFLPPDCIEKFILPNIITGYYSIEAQQLVYHPIDYEKYLVDEYTIAEDFGAY
                     FHEEDVYENFNDEELYLTWDSEQIRQFSKGEKTKIIVFGDYWYGIEREDGTRGILYFW
                     TGD"
     gene            48432..49025
                     /locus_tag="PLG49_01440"
     CDS             48432..49025
                     /locus_tag="PLG49_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16005.1"
                     /translation="MYGLKIENLALEKKAGELPGYIHHRISNEVSDFSFFFKDFVERL
                     DDYYWILEKGSTYISWESPEKDDHNFEFGLFKDFYEEVYEEYSVYRMYDNKVFKKYIH
                     YIIDDWNTIFCFKKEKIKLKDFIISINTKRGQDKENFLKENIDFIFLNIDGLFWLFFS
                     NDAFYLNDIRLSLAKFESKVKLTDCCLSDYINMIAFT"
     gene            49644..49931
                     /locus_tag="PLG49_01445"
     CDS             49644..49931
                     /locus_tag="PLG49_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ16006.1"
                     /translation="MGAEFWSCLAAMFSLVIFCYLIQLHNDILRLNRKVNKLAEHMGL
                     ADNMEPLNDEAFKELIMAGEKIKAIKRYRQLTGEGLKEASDYVDALLLKNK"
     gene            50154..50618
                     /locus_tag="PLG49_01450"
     CDS             50154..50618
                     /locus_tag="PLG49_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16007.1"
                     /translation="MIRITESRDADLLALLNYDVQEIHAEIEPMLFKHHNQENMKALF
                     KEALEDENIYAFVAWDNDRPVGYVMISKINFEENYFRKSYSVVYIEHICVLKDYKGHG
                     IGKQLIEKVKEFARDNNIRRVELDYWNKNEKAGQFFRSQGFKTFNERMYLEV"
     gene            50640..51260
                     /locus_tag="PLG49_01455"
     CDS             50640..51260
                     /locus_tag="PLG49_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16008.1"
                     /translation="MKFNVYELPLESKLYKLYDVIEVPVQLRVVYPQGKTPAIFANAE
                     GFAFLESIFKIASVSMKENCLIRIKDNSKTVESFHAWYPASTEFHLDLVIFNHQYTQI
                     SSKKIKRLLMMLDYCKKKIMNIRVPEVHYKNDNWWSLEGELHVNKRSNFLVISSNSNG
                     YLYMADEADRFCNIEDNEEEYSAHSHLFALCKEEDLLDFRYYYEKS"
     gene            51901..52041
                     /locus_tag="PLG49_01460"
     CDS             51901..52041
                     /locus_tag="PLG49_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16009.1"
                     /translation="MKILDFNKAFLEACVHLYIETFNAAPWNDHWDYETALRRFIKHQ
                     VF"
     gene            51990..52319
                     /locus_tag="PLG49_01465"
     CDS             51990..52319
                     /locus_tag="PLG49_01465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16010.1"
                     /translation="MGLRNCFKKIYKTPGFLGKVVLDNGEGKGAVMRNIEAWYEGDFY
                     DVKEFFLHKVLRRKGVGSRLMYQLCQELKDRGVKTVWLITAKIVWQRIFLYKKRLFKS
                     RKNDYDA"
     gene            complement(52336..53064)
                     /locus_tag="PLG49_01470"
     CDS             complement(52336..53064)
                     /locus_tag="PLG49_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOQ16011.1"
                     /translation="MSQKADKHSNNGHHCCHTDKRHNNQSCITLVPIFNHLERHQMEE
                     ISKSIKSLSYKKGEIIYRPGDESDSLYIVKSGKVKIYRLSESGKEQLLHILNPGDFTG
                     ELALFRQSVHESFAEAMENTNICMITRPDLQKFLEKYPSISIKMLAILSERLDAIEKQ
                     STSFATLSVEKRLVDFLIESAQNQDKEFTLPMSQKDLASYLGTTPESISRKLTALEDE
                     GYIRQLPRRKIKIINIKGLLEKYQ"
     gene            complement(53231..53530)
                     /locus_tag="PLG49_01475"
     CDS             complement(53231..53530)
                     /locus_tag="PLG49_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16012.1"
                     /translation="MRRVSEAEFDRRGKGCDKGRNSATLPANIRELLKGLIDEEVIIV
                     LKSGKYEEVEVLAVERNSLIARYDGKIKFVDIDCICEVLTDPEDFIEAIFKGDKC"
BASE COUNT        18281 a         7204 c        10463 g        17877 t
ORIGIN      
        1 acgacttaaa ctttatgaaa tagaagaacg actggacaag aattattttg tacggatttc
       61 aaactctgaa attattaatc tcagaaaagt taaaagtttt gatttaagtt ttacaggaac
      121 aatttgcgtt aagttgagta atgatacggt tacatatgtt tcaaggcgaa gggttggcaa
      181 agtaaaaaaa atattaggaa tatgaggtga gaaaatgaaa aaacaagttt tattacgcag
      241 tcttttaggt atccctatgg gaatagctgt gggatatatt attacgatta taatctctgt
      301 tggctgggga ggaggacatt attatccttg cgtaccagct ttggtgaatc ggatgaatag
      361 tgaaatttgt gcagtgatat tacaagctgt gctgtgtggg cttttaggtt cagtttttgg
      421 tgccagttca gtaatatggg aaatagaaaa ctggagcatt gtaaagcaaa cggggattta
      481 ttttttgatt attgctttag ttttgatgcc aattgcatat ctggcaaact ggatgaagca
      541 tagttttttc ggctttatga cttattttgg attttttgtt cttatattta ttttaatatg
      601 gttgattcaa tatttattct tgagaaaaac aattaataaa attaatggaa ggattaaata
      661 aaacgaaaaa ataagattgg aagaaattta gaactttacg acatatccca tggtacggaa
      721 tttaaaatta tatcggttat aggagacagg cagaactgct atctttattg cgagatgact
      781 gcagctgaag ataagtctct aaatgtttgc gaaaattcat tatttgatga aattgacatt
      841 catgttcaat gtggaaacca agtttatagc agtggatatt ccattgtaga ttttaagaat
      901 gaacgcagta atataatatt tttttaatga tattatcgtt ttcagaagaa gttgatttag
      961 caggaaatgt tatcaattta agattaaaaa attttggcca tcctgagttt aatgaaaaag
     1021 gtgaaaaatt ctttaatttt caagcggaag gggaatggat ttttgagatt cctttagatt
     1081 atccagcgta taaggcagat tcagcaatag acagagtgct ggaagtaaag gggaatcctt
     1141 caaccattat gaatctttcc ttaacaccta taagattaaa tgttgaattt ctttttccat
     1201 atactgagga tcctgatgga aaatattcat gcctgtttga cgattttgaa aactgctatt
     1261 taataacaga ggacggcgaa aagttatatg ttattaaagc ttttcatcta cagcggagtg
     1321 gaatgatgaa ttaaagacac catacttttt tcgtgtgcaa tacgaattta gtcaacctgt
     1381 taaagtagaa aatataaaaa ccattgtatt acatggcact gaaattgaca taagatagac
     1441 aattcaataa aaaaatcttt ttcactactt attgaataat gttaatagtt atgctatgct
     1501 aacagtacaa aaaataaact agcgagggat gactttatgg caaaagaatt aattatggaa
     1561 aatgattcag ctgttttatg gtatcaccct gaaagtaaaa ttgttcatca ccaaattaaa
     1621 aaatatgtgt atggtaagaa ttgcaagaat tattattaaa aggaacggag cttttgaaag
     1681 aaaaaggagc aattaaatgg ctatctgatg acagaaacag taatgccatt agaaaagcgg
     1741 atgcagaatg gggagccagc gtttggtttc ctttgacggt tcagtatgga tggaaatatt
     1801 gggcaattgt acaacctgaa aagcttattg gacaattgga tatgaaaagg cggatagagc
     1861 aatatgctga aaaaggaatt acagcaaaaa tgttcagtga tttagatgag gctttagaat
     1921 ggttagaggc tcaaaaataa agacggtttc cccccgattc aattttttat tgactggcaa
     1981 aatagaagcc agtcattttt tgtgatttga tttagtttat cctgatcttt tactgatatt
     2041 ttataattgg cttttctgat actacaattg gaaatgacag gcttcataaa taaagggctt
     2101 tttgtatctg cctttagagc agcttgtaaa aagccctgtg ttttattaaa atgacaagta
     2161 tttattttaa ttcttctcca taaattcctg catggcatga atggattccc aactgatgac
     2221 atgttcaatg gcacatgcat cttcatcggc aatttcttca tcaaccttta atacttctgt
     2281 taaaaacttt ctgataatac aatgtttatt ggcagtcagt tttgctcttt caaggccggc
     2341 aggagtcaga tatacttcct tatatctttc attaatgacc agtccttttt cagcaagggt
     2401 tgccatcgca ttgttaacgc ttgctttagt aacgcccaga cgttcggata tatcagaaac
     2461 tcttacgccg gtattatctt tagaaagctc ataaatggct tcgagatagt tttccatgac
     2521 aaaactaagt ttttccataa gactccccct aaggccaaag ttttatgaat cataacagtt
     2581 ttatttgtat tatacacttt attgatgtga aaataaagca gagtttgtaa ataaaataaa
     2641 tatttgactt attttgatat ctcattgcag cattagctgc aggactttct gtactgcagg
     2701 aagtgatttg attttaaagt cagggatata taaaaattaa aaaatattaa atatttttca
     2761 cggaatagga taactttaac aattgattat tttatgatag aatgatataa aaaaagagaa
     2821 ttttaaaatg atatgttaga tgatttttct tcagtggaag gaatacgaaa attatcatca
     2881 tgttgtctat ccaaggtaaa gaatataatc ttttattttt tttaatacgt aaaaatattg
     2941 ctgtggcggg ggaaagaatg atatcagaag tatgggaatt tgctttgata tcatcaatga
     3001 gggggtaaaa gatgataaga gctgcttgta tggaagattt taaaatactt tgtgatttag
     3061 acgagtatat ttcagctaaa gcattagaaa agaaaattaa agatggtgaa gtatttgttt
     3121 atgaggaaga ggggaaaatc ataggttggc taaggtacaa tttttttggg atgagcatcc
     3181 ctttatgaat atgctctata tcattgacgg ttatagagga aaaggtatcg ggaaaaagct
     3241 tgtcagctac tgggaagaaa aaatgcgaca ggaaggctac cattacttga tgacaagtac
     3301 cttagctaat gaagaagccc aacactttta cagaaagctt ggctataaag atataggagg
     3361 ctttcttatg cccaatgagc ctttagaaat tatgttaatc aaggttctaa gctaatattt
     3421 tgttttaaca ggacttggac aaatttaaga aagacatgac attgacgggt aaatcaaggg
     3481 atttcttatg tttgtatgaa ctttgagacg gacaatatag caactttatg gatatagcaa
     3541 aaatctaaag atgtaagaag gaattctgag gatatttatg aaacaattta ttcaagaaga
     3601 tttgaatata tagtattaat gagattcgtt aatcctctag ctgatttact tttaccagaa
     3661 agatatttta aagagcttga agagctcgga aagataaggg gaattatgtg agaaacgaaa
     3721 taaagagact tttaataaaa aacagacaaa agtaaggtaa atgacattta ccttactttt
     3781 ttatatggaa tatttcttat atttcatctg tcttttttgg attttcatta agaaaaatga
     3841 aaaactataa ataaatgctt ctatcactgt cttcttcatt aagttttgag aagatttcaa
     3901 aggagaattg tgcataatga tttataatat ccgagttaat attttctttt tcaaaagtgg
     3961 ttcttttagt acatcctgca aggaaggata ttagagtcaa aacaattata gtgaaccgag
     4021 ttattttttt catttttttc gccaccaatc aatttgatta aaaattaaat atattatatc
     4081 atttttttga cgtagtagat tatcatttgg tttcaaaaga cagattttat tttcctttat
     4141 tttataaaca acgaaaacct ttattttgaa tgtatctgaa aagtattgca tgatattttt
     4201 taaataatgt aaaagcatcc atacgattta cgggttagtt tttgtatttg ttgtagaaat
     4261 cattttgtgg taatggtaaa atgttcataa aaagtcttaa agaagaatcc tgtgtaaaaa
     4321 tgagctgaaa tctatgtata atagaaatga agggagatta cttgtatgaa tgaaaaaaat
     4381 aaaaaattta taaaaataac aggaataaca gcaattattt taatcataac agggataatt
     4441 cttttttggc ttttggatct cggttccaga ttaatcaggc atcctctgaa ttttcaggac
     4501 atgagtaaaa taaaattaga aaagcctgtt aactcagtcg aagagggata tccatatgcc
     4561 ttaaagcgtg cacgtgaatg gagagaagat gtggtattga ctgaagtgga gataatatca
     4621 tctgcaatag gcacaataga tgagatagat gatatagaag acagaaatga aatcataaga
     4681 tatacttttg agtttgaata tatgaatgaa aaacaaccat ctggttttat cttagtttat
     4741 cttaatgtaa agacaaattg catagatatg gtatcggcat atcatgatga taataatttg
     4801 ttttcaccaa aagatgaatt agatccagaa gctatatcag ataaaattgg taaagtttat
     4861 gatgctgctt taaaagcaat tggaaaagag aatattttgg gaagtgcggg actctatttt
     4921 agtgcaaaaa ttaattcaaa ttttgctgtt gtatctgttt atcgcgagat agataatgag
     4981 gaagggaatt ttgaaaatta tgagataacc attgatatgt caacatttga agtaacagaa
     5041 atggaaataa aagaagcccg tcgggaagag taggaggtaa tggttgtgat aaaaaaagga
     5101 atgatgttca ttattttaat gagtgttttt gtattagtaa tttgtagctg tgcttttaca
     5161 ggagatgaaa aatcagagac aaaaaatgtg caagaacaag ttaatgatgc agatgcggag
     5221 tttagaacag atatagaacc cataagcaaa acattcccta atttagggga agttttggaa
     5281 tgttactgga aagcggattt ttttggcggc gaagcttcat tcagagtgcc tggacccaac
     5341 ccatattgga ttcagggctt tgcgaaaata agtgaagatg atattaataa atttaaggaa
     5401 gaatacgact ggactttagt tgaagaaggc aagaagccgg acttagagac agaaatttta
     5461 aatttagatg aggaggactt tgaatggtat tatagcgagg aatttaataa gtatgttaaa
     5521 ccagcaaaat attctggtgt tttttatatc gattttaaga ataacattat ttatttttat
     5581 gtatgtgtat aatgatattc atattactct gatatcatgt tataaaaatt gtgttatgtg
     5641 tatttgtcaa agtaaatgca aaacaagatg tttttaattc gttcatgttc caataagaat
     5701 gaggcaaatt ctgatttaac gaatcaattg gcagttgcaa gcggaaggat caacggagga
     5761 gatagatttt aaaagttaca gcagtcatta gtattcagag gattatacat agaattgggt
     5821 gaaagcgggc ggacagtttt ttctggtacg gaaattattt tagatatgga agatgaaatc
     5881 attcaaatta ttacaacttc aaaagcacca acatttaaat ctttccagaa ttaagaatcc
     5941 acagaaaaag ataaggaaag aatgaaaaag tatgaaaagg ctatggagag aatagatcaa
     6001 gcattcgctc ttgaagagag tgctggggat gagcatatca ttcaaattga tgggggtgtg
     6061 tttatagagc catatttgag tcatgatgat tttttagttt aaaagtccca atattaagat
     6121 gatattggga cttttaaaaa gttatttata aatttttaca tcaacattaa agtttggacg
     6181 taaaaagcta tctttattat caacggagat ttctattggc actacggttt ctccattttc
     6241 tttaatagcc atgtttgaga tttttgtaac ctttcccttg tattcttttg atgagtcggc
     6301 cagtaagata atatcaacag gggcacctgc tttaacgtct ttaatgaatt cttcagatac
     6361 gttggcttca attatcaggc tgtctaaatc gagtaagcta agaacttttt tgctttggtc
     6421 agtgttgtca ccctcagtat aaccaatttc atatacaacc gcatttttta aatcggatac
     6481 gattttatct tctttaataa atggtttatt cattttttct tttaaaagag caatttcatt
     6541 ttctaaagaa aggattcttt gtttttggat ttcgacttcg atatttgagc tatttaaact
     6601 tgcattggtc aggtctaagc ttctttccaa ttcactgatt tctttttgtt tgttattttt
     6661 taatgcttca acagaaagtt ttaaggattc aacattattt ttactttgtt caagatttct
     6721 ttttatctta tcaaattctg attctgagat agcaccttgt tgatataaaa tctgttgttt
     6781 ttcatagtcc ctttttgcat cttcatagct tttttgggct aaatccaaat cgttcattaa
     6841 tttttggata tcggggtctg aattactgga taaggccttt ctcttatttg ctaattcgtt
     6901 ttttatcctt tgggattcct tttgactaga aggtgtttct cctgcctgga ttctttgcag
     6961 ttctaattgt gcaattctat attcgttttc cttgtcttgc aattgcttat tgtaattgga
     7021 aagatcaagg gtgattaaag gatcatttaa gttaactttc tgaccattgc ttacatgaat
     7081 tttttttatg gcagcaggga aatccagatt aatattttta attttccctg cttttataat
     7141 cccaaaagct tcaatgtaac gatcaaccgg aggagggagc tccgttgttg tttcttgtgt
     7201 ttcgtttttt ccgcaagctg tcagcaggat gaatgtattg attaaaaaga agagaccgat
     7261 cttttttatt gtcattgatt ccacaccttt ccgtctttca ctttaataat ccggttaccg
     7321 tattctgctg cttctgttga atgggttacc tgaacaatgg ttttgttttt ttcttcattt
     7381 atttttttaa gaagaatcat gatatctgtt cccgttttgc tgtccaggtt accgatgggt
     7441 tcatcagcca gaatgatatc cggctcattg atgagagccc ttgctatggc aactctttgc
     7501 tgctgtcctc cggacaattc ccgcggagta tgctttcttc tgtcctcgag accaacaatt
     7561 tctaagattt cattgagttt atctttgtaa tttttaattt tttttccatc aaggagaatc
     7621 gggagcatga tattttcttc aacgttcaga ttagggatta agttgtaaaa ttgaaagaca
     7681 aagcctattt cttgtctcct catcgtactt tcttctttat cgttcatgac agacagttct
     7741 ttaccattaa ttttgatgct gccggaagtt ggtttatcca atcctcctat taaatataaa
     7801 agtgtgcttt taccagagcc tgaaggtccc atgatagaga caaattctcc ttttttgata
     7861 cttatattta tatcttttaa tacttctaca ctggtatcgc ctaattgaaa agtcttacat
     7921 agatttcttg tttcaataac attttcaata tgattttcca taaacttacc tccagttttt
     7981 attcatattt aatggcttca attatattgt attttgaaga tttaaggtta gggcttatag
     8041 aagccataac cattaccata gtacctatta taaaagaact aacaaataag gaaggagaga
     8101 gatgaatgtt cattggtaaa ttcattgctt ctaataaatg ttcaatgaca taatttacaa
     8161 aaatacctcc aatgacacct gttatacttc cgataaggcc tccagtcagg gattcgataa
     8221 agagcatttt tgtattttgc tttttttcca ttccgatgga gcgcataatc gcaagctgcc
     8281 gttttctttc taaaaaacta atcaaaagat tatttaatac cccgaatata ccaataatga
     8341 cggtcataaa ggaaaaaccc tgaagaagcc ccatcatttg tgaattagac tgcataatgt
     8401 cttttcgaag ttgattgaca gaattaatat aggggtaatt tcttttaaat tttttattta
     8461 agatttcaac catttcttct ggatctttag aagttttgag agccaactca tcgtagaaag
     8521 ggctatccat atcgttttta taatattttt gagcaatgag tgcatactga ccattataca
     8581 taagagtaag agcgttaaaa aagcctaaaa cagtgtactt tcgctctcct tttttcatgt
     8641 ttaaagtcaa agtgtctcct atgtttactc catgtttttt cccttgggcg taggttagaa
     8701 gaatatacct tcctgtatcc agttttttta acaattctgg ttcgatatca tagtcataga
     8761 aatccaggaa ccgactgaca tctattcctt gcacatagcc caggctttga ccgctttctt
     8821 tgaccaggat gttatcataa cttccatata ttccataact ggcggctatt ccaggggtag
     8881 actttattac ttgctcaaat cttctatcca ttccatagat ataaaactga atatcataat
     8941 ttaactcttt ataagcatct aaaacttcaa tattcataga atagtttact gaattaatca
     9001 taataatgct ggcgatgcca atagttaaca gggatatatt atttaaaatg ctcttgtttt
     9061 ctcttaaatt ttttgcccct aaaactccga tatttccaaa gaaaaagata tatatttttt
     9121 caaacagaca gataaacact ttgcttaaat aaggtatgag ataaataaga gcaacaaaaa
     9181 ctgcaatcat taaaatcgca ttaattataa aagcaatgct gtgtgggata ataggcggta
     9241 aaatcagtgc tcctgacaaa aagcctattc caaagtacag tttccatttc tttttcttat
     9301 attttttttc gatgttgtta agaacaatat cctttactgg tatttttgat atttttaaaa
     9361 cgggtaataa agcactgaga cttgaaagaa caatagctaa ggtgagtgcc tgccttaatt
     9421 gcccaaaggt aaatttaata ctgacatcaa tctgcatata gggatcattg gctataacag
     9481 cagccattcc atataaaacg ccaatcccta aaacacagcc tgatacgcct cctatgattc
     9541 cataaaggat actttcaaga aggagtacgc cactggtcat ttttttagta gcgccgatgc
     9601 ttctgaaggt tcctattaca ggcaggcgtt ccatggtaat tactttaaaa gaggtataga
     9661 tgataaatat actcataaat aagacaagta aagtgatcag taaaaaaggc gttctaatca
     9721 tactcaattg ctgaaggatt tcttcttcag aaaaaaattc tcgcacggta tagcgattat
     9781 aaagggctga cagttcattt aaaatttctg gaatgttttc gccttcagcc gttttgatat
     9841 acaataagaa agatttacct ctggcttttg ctaaagatgc aatcgtttct cttggaataa
     9901 taactggtat atttctgtcg ttttctttta agagaccttt tgaagctgca atagcagata
     9961 cgcgaaaacg ttgctttgtt ccttgaattt ctaaatccat tggactgcct aactctaact
    10021 tatatttttc agcaaaagtc tcactgataa tgatttttct gccagtaaag ggttccaggt
    10081 tttggctgct aataagatgg atgggattca ttttttggag ttcaccgtag tcatatccta
    10141 ggatattaat atttaaaata tcatctgttg aagctttata ataacctgtt aattcaatgg
    10201 ttccaatgac atattcgagt ttattgccta agttttctgc aggcttagga tccacataag
    10261 gtgtgggaga cagttcattg ctatagatga taatttctgc acttccaatt tgggttctca
    10321 tttctttgat tagcatatcg gttaaagtgg ttgataaggc attggaagca aaaaaaagag
    10381 ctgtagaaag tgttatagaa aatataatta gaaaagtccg taattttttt tctttaatat
    10441 ttttgataag gaattttatg attatcccca ctactgcacc tcaattcatt ccaaattact
    10501 tttgtatata cgatagataa ttacaaaagt ttatttaaat tttttatttg gtattatatt
    10561 caaaaaatat gatttaatca gtatttaaaa agggattttt tatgaatcat taaaagatga
    10621 cttttttaaa aaatataatt aatgagttgg aaatatggca gaatagtacg aaatatgtta
    10681 agatgaaaat aattgtgttt tataacagtg aattatatta atagataata ttaatatttt
    10741 aggagaaata acaatggttc aaatggatga gttaaaagta aagcttcgag aatatggaat
    10801 tatgtgttat gaaaccatta agaaagacgg ctttccaaca atatatatgc cagataatga
    10861 cttagaatcc tttcttgact ttgttatgaa taaccaaata aagaattttt ttaaaagctc
    10921 agatattatg taaaagatca attttatatt catgaagaag atataaaatc tttcagttta
    10981 aaacagatat ctcaaaagat ccagcctata gatttaacaa agagtacaga gataaaagaa
    11041 gcagtcgtca aatataatga gtcattagat gctatagatt tttcaagacc ttctggtatg
    11101 gaattatatt gtatatacga gagcaaatgt atcgctgtca atttttatga cccatgacat
    11161 ttagaatttg gattgctgcc cagacatcaa aaattagcag agattattaa caaacaagtt
    11221 catgtcatag aaagaatata tcaggagcag cgtttaaaag aagaacaaga gaagaaagca
    11281 agtgaaaggc ttttagaaga attcaaaaat ttcgttttaa atgatccgga ttttaaaaca
    11341 tgtacttttc cgggtgcaag acgagattat gcaagagaag tgtttacaac aagaaaagat
    11401 acagccaagt ataggcacct gttttatgca gaagatgatg tggattatat agataaccaa
    11461 agggcgtatt attttataga aagtatatgg agggaatatc ggtccccaaa ttagaagcat
    11521 ttggatattg attgcaaata ccttgtaagg agatttagaa atgttttaat aaatggttct
    11581 tgaatatttt agtcaattgt gaagggattt aaggaattat ggcaataaaa atatttatat
    11641 ggatttttta ttttggagca tttattagta ttttgaattt ttatctttca tggattcgtt
    11701 atccaattta ttgtttgtta agaaaaaagg aatcttataa atttaactcg gggattcctt
    11761 tattgggtag tttatttaat atcatttcca ttattactta ttggggcact gcaagagcag
    11821 ttttagcatt tatctttttt cttattgaca caggaggatt tccatggtta atttatagta
    11881 tggcaaagga aaattggtgg agagaagaaa aagataaaag agaacgaaaa aaataagatt
    11941 ttcttttgca tttatggact ttgtttgctc attacatctt gagtagagaa gtaaaatatt
    12001 atgagttttt tcaattatta taccaatatt gtaaaatgaa gaaagatttt ttattcaaaa
    12061 atagcatttt tttcaaaata ctctgttgaa aacaataaaa atttagataa aatgatatct
    12121 ttacatccca ggcttttagt tttaaaatgt agtattatca atactttaat aaagggggaa
    12181 cctaaaatag acaagaagaa tgttattata atcggagctg ctggacgtga tttccacaat
    12241 tttaacactt atttccgcaa caatccaaat tacaatgtag tagcatttac tgctgctcaa
    12301 attccggata ttgataatag aaaatatccg gcagaattag ctggtgattt atatccccag
    12361 ggtattccaa tttattctca ggatagattg ccagaattaa tcaaagaact tgatgtagac
    12421 gaatgtgttt ttgcttacag tgacgtgcca tatcaaactg taatgggcct ttcagctatt
    12481 gtaaatgctg caggggcaga tttcaagctt cttggaccaa aacatacagc acttaaaagt
    12541 aaaaagccag taattgcagt ttgtgctgtc agaactggtt caggaaaaag ccagacatca
    12601 agaaaagtta ttgaactgct tatggcaaaa ggtcttaaag ttgttgcagt tcgtcaccca
    12661 atgccttatg gtgaccttaa tgcacaaaga gtacaaagat ttgcgaccat tgaagattta
    12721 aagaaacaca aatgtaccat tgaagaaatg gaagaatatg aaccacatat tacccgtggc
    12781 aatatcattt atgctggtgt agactatggt gacattttac gctcagcaga aaatgatccg
    12841 gatggttgcg atgtaatcct ttgggatgga ggaaataatg acttcccatt ctatcaacct
    12901 gatttaacca ttacgattgt tgacccacac agaccaggtc atgaattaag ctattatcct
    12961 ggtgaagttt gccttagaat agctgatgta gcggttatta ataagattga tagtgcaagt
    13021 cctgaagcga ttgctacagt agaagccaat attaaaaaag taaatccttc tgcaaaaatt
    13081 atcaaagcag aatctaagat tatagttgat aatcctgatg ttattaaagg taagagagtt
    13141 ttagcggttg aagacggtcc aactttgacc catggtgaaa tgaagcttgg tgcaggaact
    13201 gttgctgcag aaagatttgg tgcaaagagt cttgtagatc caagacctta taccgtagga
    13261 aaactcacag aaacctttaa gatttatcct aatattggta cacttttacc tgcaatggga
    13321 tacggcgaac aacaattaaa ggaccttgaa acaaccatta acaatacgga ctgtgattca
    13381 gtgattattg gaacacctat tgatttaagc agggttatta atatcaataa accttatacc
    13441 cgtgtttatt atgatttgga tgaagtttca agtccaaatc ttaatgacat cattgatgag
    13501 ttcgtaaaga aatttaactt agaaaaataa taaatttgaa ttaattgtgg gatgaaaaag
    13561 gcatcgtggc taatcacgat gcctttttta tttacaacaa tgttttattt tttggtagta
    13621 tgcattatta aaaagatata aaagacgata tatagtataa atgtattact ttaattaaaa
    13681 tatctgtttt ttaagtttaa acataaagtg taaaacgaaa aactttatag tttacaatta
    13741 tttttatata aatagaaatt tagtgaaata atattctgga aggaggtttt tatatgacaa
    13801 atgtaaaatt ggctattatt tattacagca tgactggcac gaattatcaa ctggcaagat
    13861 gggctgaaga aggggctaag gaagaaggtg ctgaagtcag gttattaaag gttcctgaga
    13921 ttgcgcctca gtctgtaata gacagcaatc ctgcatggaa aacacatctt gaagcaacca
    13981 aagatgttcc agttgcaaca acggaggaca tggattgggc agatgccatt attttcagtg
    14041 cacctaccag atttggcggg attgcttcac agatgaagca atttttagat acaaccggtg
    14101 gcttttgggc acaaggcaag actgttaata aagtggtcag tgccatgaca agtgcaggta
    14161 atgtccatgg tggacaagaa gcaaccattt tgtctcttta tacatccatg tatcactggg
    14221 gtgctatcgt tgtaacccct ggttataccg atgcttctat atatgaggct ggcgggaacc
    14281 cctatggaac aagtgttgct attgataata gcgggaaaat gaaagatgat gtagaaaagg
    14341 cagttaagta tcaggcgaaa agggtcataa aagtggcagc tgctttaaag aaaggttggg
    14401 gtaattaatt taaaaagccc atgtcattta ttgacatggg ctttttaatg aatagacatg
    14461 tctgttttaa ttatcagcag tacttgttgg tggttcagct tctgccaaac ataccagagc
    14521 gctttctaac cgtcgttctt taatttcagt gactttaatg attaaaccat attcttcgag
    14581 ttctggtgta ctgccatcgc tggggactgt tcccaaaagt ccgaaaacaa ggccagcaaa
    14641 ggtttcatat tcttcttcag gaagcatcac accaagttct tttgatactt catcaagagg
    14701 agcggtccct tgtattctcc aggtttttga atcgatacgc tcaatgggtg gtgcttcctc
    14761 aggtatagaa atatcatctt caaggtctcc aaccagttct tctaataaat cattcatggt
    14821 gatgattcca ctcataccgc catattcatc gactacaact gcaaagtgat ttcttgattt
    14881 tttcatgtta cggaataaga cgtctgcccg gaccgtttct ggtacaaagt aaggtttttg
    14941 tacagccttt ttcatgacct cactacggtt tttattttta agcctgaaat agtctttggt
    15001 gtgtaaaatt ccaacaatat tatccgggct gtctgagcaa atgggatata cagaatgcct
    15061 gctttcatta atagtttttt cccattgcgc atcggattct tcaagccata agagtgcaac
    15121 ttcagtacgg tgtgtcataa tttctgcggc agagatatca tcaaactcaa agatgttttg
    15181 aatcatgttt tgctcgtccg gtaatatagt acctttttgc ttacctgcat ctaataacat
    15241 aaggatttct tcttctgcat tttcttccgt ttcgctgttc ggatcaatac caaaaagcct
    15301 taaaatactg tttgtagaaa cagtaaacaa ccaaactgct ggggtaaata atcgcgaaag
    15361 cccgtaaata aggctggaca tgccaagggc aattttttca gcatttttca tggcaagtct
    15421 tttaggaatt aattccccaa aaagtactgt aaagtaagtc agtaaaactg taataatggc
    15481 tacagagaaa gcattaataa tggcttttgg aatattaaga ttaagatcaa tgaagaaatt
    15541 taccaggcgg tctgaaaagt tttctgttgc aacagcactg cttaacaggt taaccaaagt
    15601 aatacctact tgaatggttg aaagaaaagc agaaggctgt tctgttaact tactaagccg
    15661 taaagccctt ttgtcaccgg atgcagcaag ttttgccagc ttattatcgt tcattgtgat
    15721 aacagcgatt tcagcacagg caaacatagc attaaaaaag ataaatacga gttgcagtaa
    15781 aagcaaccaa ataaaaggat tatcgtccaa attataccct cctaaaatac atataatagt
    15841 gttttgattt tttaatgtta gaaaagattt aaaattaaaa cttttattac aataataaaa
    15901 aaaagcacaa caaaaaggac aatacaggat tgccatttgt catgcggtga tttgttatgc
    15961 aattagctat atttttatat tttacaggtg accaaaattt attaaatacc tcattaggag
    16021 gtacagcttc agagtcagca gaataatcca ctgtaaaatc actcctttta atacacaagt
    16081 atactaaact tttttatgaa tgtcaaatgc ttatagcctt taatcatatt ttttactttt
    16141 gttataaagc aaaaacaaga agtttaattt aaaaacatta aaagtaaaat taagtttcag
    16201 ttataaataa gaatggaaaa gcataaaaag acaatgagaa ttgttttcaa aatacatcaa
    16261 aaatattgac aagaattaca caaagtgatt taatataatt gtcaatatgt tagagagaac
    16321 taacttttcg cataagaact tagttttatt atttgtaagg agatgagctt aatggttaag
    16381 cagcaggaat ataaagtaat aagaaataca gttagggttg agacagaaga aaaaggtcaa
    16441 agcatatatg atgcacctga ccatatgaaa tatattataa ccagcgctcc tcaactgggg
    16501 attttaaaga gtttgggtat tgttaaaaat gcatcagttt ataaggagat gacgcatcgc
    16561 tttggtggtc ctgtgttgct taaggtagaa tccagtaaga ttgctatagg aaaagatatt
    16621 gctgaaaaga tcctggtgag gaggtaatgg tatgggagaa tgtcatgaag ttatcagtga
    16681 tgaaaatata tacgaaaagc cagataaaat tcttttaatg gggaatccca acgtcggtaa
    16741 gagtgttttt ttctcacatc ttacaggaat tcatgttgtc agttccaact atgccggaac
    16801 aacggtaact tacacggaag gaacgcttca attaggagat aagacttaca cattgataga
    16861 tgtacctgga gtgtattctt tagaagccac atcggaagct gaggcagtag ccgtaagttt
    16921 tttgaacagc aatcctgtgg ctattttatg tgtattagat gcgacgaatt tagaaagaaa
    16981 tattaaactg ggtttggaac tgaaaaaata taatgttcct attgtttatg cattaaatct
    17041 gttagatgtt gcgaagcgtc atggtgtaga gataaacgtt ggcttattgg ctaaagaatt
    17101 agaagcaccg gttattccga cggttgccgt aaaagatgaa ggaattaacg aagtaaaaga
    17161 agctttaggt aaacttttaa agcattcttt cctcaactgc agtcactgca cttcttgtcc
    17221 tgaatgtcca gggataaaaa aagacgaaat ggagttctgg aacagggcaa gggaaatcac
    17281 tgctaaagta aggaagaatg tagatagtaa attaagcttt ttggacagat tagggaatag
    17341 catgttaaaa ccatggccgg gtatcccaat tgcactgctg gttatattat tatctttagg
    17401 agttattgta ggtggcggta aaattttaca ggaaggtttg ttactacctt gggtcaataa
    17461 aataatcgtt ccatttttta agtcaatatt tgcttctttt ataccagaag gtattttatt
    17521 aaatattctg attggtgaat atggaatatt tgtgattggt ttcgaatgga ttataacctt
    17581 aattcttcca tatgtatttc tattttatgt agtgttttca tttttggagg attcaggtat
    17641 attgccgcgt ttaagtgttt tatttgataa tattatgcgt aagatgggcg tgcagggggg
    17701 cagtatgatt actattttaa tgggttatgg ctgtgctgta ccagctatta ttagttccag
    17761 gactgcaaca acaagaaaag agcgtttaat tatatctaca gtagtatgtt ttgcagtacc
    17821 ctgcatttct cagacagggg cattaataag tttatttgct gctttttcgc ccttattgtt
    17881 aatcttaatg atacttttgt ctttagtcat tatgatgatt gtatctggtg ttttaagccg
    17941 aatgctaaaa ggaacagttg atccaatggt tattgaaatt cctaatttat tgatgcccaa
    18001 tgcccatgcc tatgggaaaa aacttatgat tcgcatgaaa tattttctgt tggaagcaga
    18061 agtgcccatg ttaatatcta tccttattgc agctgtgtta aaagaaacgg gcttattggc
    18121 ttatattgca gcatatcttg aacccattgt gagcggatgg cttggcttac ccagggaagc
    18181 ggttattgca ttaatcttag gaattgtccg tcgtgagatg gccgttacgc ctcttttagc
    18241 tattgaagga ctgacagccc tacaggcctt tgttggtggt acagtggctt tattgtactt
    18301 gccttgtctg tccgtatttg gtgttttgac caaagaattt ggacctaaag tggctgtggc
    18361 aattgcctgt tctaccgtag tcactgcatt tactgtggct ggattaatta atcaaattgc
    18421 acatattttt atataatttc tgtgataatg cggatttatt aaattttggt ctattaagct
    18481 tgttctgtca gacagagctt aaaaagacct ttttttattg atttaaatca atttttttta
    18541 aaaagtttca tgatagaatg aatacagaaa gggaaagaat gaataataag ataagaaaga
    18601 gggagatatc atgtcaaaag aattacgatt taatgaacta aaagaagaac atttgaaaat
    18661 attagggcaa tatgtgcctg ttgttgcaaa agtacatggt gaaagtcatc ctgaatttca
    18721 tgaagtacac aaaatttttg aaaagataaa cgaaaaaatt aaagcagggg atttggaaaa
    18781 gcccaattta gatgaagaat ttgcaagttt gcgtaaagtc acgaataact atacggttcc
    18841 tgctgatgta tgcgaaactt acgaaaaggt ttacaggctt ttggctgagt tggatcaagc
    18901 ttatcaagcc tgaacggaaa agtgataatt atgaaaaaag taatcttaaa gcaaaagaat
    18961 caaattgctt ttatcagtgg gattttaatt gctttagcat ttatcagcaa acttcttttt
    19021 aaaaatgaat tagtctttaa tggcctgtta ataattgctg cttttctggg tgttagtccg
    19081 attgccattc agggttttca ggctttaaag gttaagcttg tcagtattga tatattggtg
    19141 acgattgcag ttcttggggc atttgtgatt aaaaactatg aagaagcggc tattgttaca
    19201 tttttattct tgtttggagc atatttggag caaagaactt taaatcaaac ccgttctgcc
    19261 ataaaagaat taactgaaat ggcacctgat acagctttaa aacaaacaga agctggagaa
    19321 tttgaagaag ttgacctgga tgaagtggat gcaggagata ttttattggt caaaacagga
    19381 gcaaaaatac ctgttgacgg cagagtttta acaggtgagg gccatgtcaa tgaagcaatg
    19441 attacaggag agtctgttcc ggtcagtaaa aagaaaggtt tcaaggtata tgccggaacg
    19501 attttagaaa atggaaccct acaaatagta gctgacaggg ttggggaaga tacgactttt
    19561 ggaaggatta tagaattagt ggaagaagca caagattcaa aatcgcaagt agaacgcttt
    19621 attgataaat tttctaaata ttatacaccg gcagttttac tttttgcctt tgtggtttgg
    19681 attttttcca aggatattga tttgtctatt accattttgg ttttaggttg cccgggagcc
    19741 ctggtcatcg gggtacctgt ctcaaatgtt gcaggtattg gaaatggagc aagaaaaggt
    19801 gttttgctaa aaggcagcga agtaatccat agttttagta aggtagatac gattgttttt
    19861 gataaaacag gaacccttac catagggaat cctaaactgg cggatattaa attttatacg
    19921 aaagacgaga cagaagttct taattatctg gcaagtgttg aaaaagaatc ggaccatcct
    19981 ttggcaaagg caattataga atatcttgaa ggtgccaggt tctatacggt tgaacagacc
    20041 aatgttgtaa aaggcggtgg aattattgct tatgtcaatg gtcgcagaat tgcagtggga
    20101 aatctagaac tgatgaggca agagaatatt cctttagatg aacatattat aaaggatatc
    20161 tgtcagtttg aagataaagg caattctttg gttttaacgg ccattgacgg tcagttaaaa
    20221 gttttaatgg gaatccgtga caaaatccgt cctgatgtta aggaaaattt acaaaagctt
    20281 aagacattag gggttaaaaa ccttatcatg ctttcaggtg ataatcaggg gacagtggat
    20341 ttagtagccc gggagcttgg actgacaaaa gcttatggaa atatgctgcc agaggataaa
    20401 tcagcttata tcaaaaaact acaagcaaaa gatcagattg ttgcttttgt tggagatggt
    20461 gttaacgaca gtccatcgtt agctttggcg gatataggaa ttgccatggg aaatggtacc
    20521 gatgtagcta ttgaaacttc agatattgtc ttaatgaatt ctgatttcag ccgcctagtt
    20581 tatgctttag gtctggctag agcaacagtc tggaatatgc gtcaaaacat tattatagct
    20641 gttggagttg ttttcgtttt actttttagt gtgtttttta gtgattggat gaatatgtca
    20701 cttggtatgt tgattcatga aggcagtatt ctggctgtaa ttttaaatgg aatgagatta
    20761 gttcgttata agctgagata agaattaaag taattcaatt taatcataaa aatcacaagt
    20821 gataattatg ttaggaggga tagcaatgaa aaaagcaacc attcaattag aaaccttagt
    20881 ttgtccatca tgctcgcaaa aaataacaaa tgggataaag gctttggaag gtgtcgatga
    20941 agaaagtgtt acagtatctt tcaacagcag taaggtcaaa ttaaattttg atgaagaaaa
    21001 gctttcaatt aaagaaatag aagatgctat tagtgtttta ggctatgaag ttatcaagtc
    21061 acaggtgaaa tagaaagttg acttataata taaaagtcag tgatttttaa tatatttaaa
    21121 tagaaatgct cttttagata aattctaaaa gagcatttct atttaaaagc atcttgaaag
    21181 tatattttaa gatgtctgtt ttttgcagtc agaaactggt taactattta ttgaatatta
    21241 aaaaatattc tttatatcat ttagaatatt tggtaatata taattgttat tatttttact
    21301 ggtaaggagc tttcatatgg gggttatggg aatagcagag atcatggacc gtgctgttaa
    21361 tatctttaaa aaaaggattg gatattttgc gcttttttca ttgatatctg gaggtatttt
    21421 tattgttctt tattatttga ttacgacacc tttttctttg ttattattat ctttttcaga
    21481 gatttgggga ttattactct ttattgcagt tttgtttctc attcttatga tttttgcatt
    21541 ttcatatgct attggtatta tggagattat cagacaagat atctttttta ataaactttc
    21601 tgttgcatct gctatagcca gtgttttcaa aaatattcct aaaactatag cgatagttat
    21661 tattaattgc atactctttt tcccgcttat cgccgtatat attttctttt tatattattt
    21721 tgatgtggtt ttatcagaaa atattattat cgatcttgtt acgacggatg ctttatatat
    21781 tataatattt gttatccttt gtcttttagg caatattatt tttgtagctt ttatgggatt
    21841 tactttattt tgtttatttg tagtggtaga agaaaactgt ggaatcattg atgctttgaa
    21901 taaagcttta tggttgataa aaaatgagat ttttaagatt ttatggtctt taactgttat
    21961 tgaaatgacg gtcttttcta tttatttgtc tttaatcagt ttttccctaa ttatcagtgg
    22021 tataatcatt gccatatgta tgtcattttt taaattaaat gaacttatga caaccttatt
    22081 agtgctgcct ctttcgcttt taaattggtc actgtctcag attactttct taatcattgg
    22141 tattttagca attatcatgg cttgtttatt atgttataat caaaaattta aaaaagaagg
    22201 ttatgatatt attataagac ttacgcagtt agaaaatagg aagaggagcg taaaacagtg
    22261 aatctgttaa tcatgaaaac accaacgaaa cctgtatttg atgaaacggt aaagaaagtt
    22321 ttaatgaaac atggctatat ccgtgaagaa aaagagccct tagatatttc tttacctgct
    22381 tttaaccttg ataaaaacgt tatgatagtg gttatggtaa tgattattat gttgcttgtt
    22441 actgtgttaa ttatccaagc caaaaagtct tataaaagaa aaaaacagat aaaggaaatt
    22501 atgggggtaa aaattgaaga aaatacgaca ccatacagct taaagaataa agcaaatgtc
    22561 catgaagaaa gaggagaatt cagatatgct atccgtttta attatattgc attgctcttt
    22621 ttgatgcatg aaagcaatgt tttgtattta gatgagaccc aaaccaacaa agaaatcata
    22681 cagtatctca tgaagaatgg ttttaatctg gtgcctcaaa tgtctttttt aatggaatat
    22741 tttaaccatt attggtatgg gcataaaaaa tgcgatatgg attcctatca acaatggaaa
    22801 aatgttttag aaatgctttg gaatgaaatt cagtcaggaa aaaataaaac gagtaaaaat
    22861 acggtttttt ccacatgcct tattttggca atcgttctat ttacaactgc ctgttcggga
    22921 agctcgggac cttattcagt taaagataaa ggtccctttg gtcttagcgt attctatatc
    22981 actttaaaag aattggatta tcctgtaaaa agaagtctga agcctataga gaaccatgga
    23041 cataatacag ttcaaattgt agcagataac aattatttag atatcaataa tgaaaagata
    23101 caagaatggg taaaagaagg aggaattata gtatttttga aagattcgac ctccgagaat
    23161 atcctatatg gagaaattaa agaagaactt ataggcgaag tatattctta tggtaaggga
    23221 tatattattg ttaatgatgt atcagttgtt ataaatggta tgttgacaga gtcaacggag
    23281 gaagcctata acttgttaaa acagattgat cagttttcct atgaaaaaat atattttaat
    23341 gaagaacact tctattcaaa aaatgaaact cagaatgaag acaagacaga agaagacaat
    23401 gaactacctg acttaaacac agaatcactg tggaagtact tgccggcaag tgtaaaaatg
    23461 ataattttcc aattaattct agtgatgatg gcttattttt actttaaagg caagcgattt
    23521 ggaaagatta tccctttgta tgaagaagtc gaacgaagtg aaaatgaata tttatattcc
    23581 gttgcttatc tttataaaaa ggcaaaatgt atggatatta tattagagat ttattataaa
    23641 agctttatta gaaaaatccc catggcgtgg catgatgatt ggttaaaata ctgggaagaa
    23701 gaaggattgg cttcttattc ccaggcaaaa aagatatatc atttgatgaa taatcaggaa
    23761 cgaaatctta acagaaaaga attgatatct tatattaata ccatagaaaa gctaaagaaa
    23821 gttttagata aaaggagaga tttatattgg aaaacgctga aatcagattt ataaaatatc
    23881 atattgacag aattaaaaaa gaattgagaa aagttattat tgaacaaaat gaattattag
    23941 agtattcatt gattgcttta attgctggtg gtcatgtttt attggaaggg gttcccgggt
    24001 tagcaaaaac ccttatgatc cggagccttg ccaagaccct ttcagttgac tttaagcgca
    24061 ttcagtttac cccggacatg atgcctgctg atattacagg gacaaaagta tttaatatgc
    24121 agacaaggac attcgagtta aaaaaaggtc ctatatttac caattttttg ttagcagatg
    24181 aaattaaccg tacaccgcca aaaacccagg caggcttgtt ggaagcaatg gctgagtcca
    24241 gtgtgtctat tgatggggaa gttatgcctc ttcctaatcc ttatatggta tttgcaactc
    24301 aaaacccact ggaatatgaa ggaacttatc ctctgccaga agcattggtc gacagatttt
    24361 taatgaagat agtaattaag tatccttctt tacaaggaga aaaagaaata cttaaacgcc
    24421 atcatgaagg tttttccagc gatgatttgg atcgagccaa tattgaagca gtatgtaaga
    24481 aagaagaaat tttagcgtgc aggcaattgg tttcaaaagt caaagtggat gagagtttaa
    24541 tggaatatat tgtacaaatt attgcagaaa ccagaaataa tcctgttatt gaagtcggta
    24601 gttcaccaag aggaggcatt gctctgctta attgttctaa ggcatgtgct gcatattacg
    24661 gaagggatta tgtgattccg gaagatatta aaaatatggc gattccaaca ttaagacaca
    24721 gaattatatt aaagcccgag gttgaattgg aaggcattac agcagatcag gtattgatga
    24781 atattttgtc aaaaatcaaa gtacccaggt agagagtagg gatggccttg aatataacaa
    24841 agcgctatat ttatttatta tttttaggag ctgttatttt aacggttttt tcctttttaa
    24901 attcctggct tattagtttt acaatatttc tgatttataa tggtgtatgt tttttattat
    24961 taatgataga ttattttacc agtccgggaa aagaagtttt tgaaatatcc cgatggggta
    25021 ataatcatct ttctatttat gaaaaagaag aaatagcttt tatggtatat tacaaaggct
    25081 ataaaaagac cagggtagaa atgaaagatg aaataccaga ttttcatttt aaaacagata
    25141 agaaagtaat aaaagctgaa cttttgcctc atagcaaaac aaaactgact tatcttgtga
    25201 caccaaccaa aagaggggca tttctttttg gtaagcttca tgtacgttat gaaggtaatt
    25261 ggaaattatg taagaaagca tttatagttg atttgagctt ggaatataaa gtttacccta
    25321 atatgaaaaa cttacgaaaa tacaggatga caggattaaa taaccatcag tggaagcaag
    25381 gacagaagca tttaaaaatg ttaggtaaag ggacttcttt tgaaagtttg agagaatatg
    25441 ttccagggga tgaatacagg aaaattaact ggaaggctac agccagggga gataagccaa
    25501 ttataaacca atacgagccg gaaaaagacc agcatgttta tttttttatt gataccggaa
    25561 gacctatgag ttattccatt agaggttata acaagttgga cattgtggta aatacggctt
    25621 taatattatc tgatgttgtg aatcaaaatg gtgacttatc gggcttatta ttgtttaata
    25681 cagaagtgga taatatgata gctcccggga aaggaataga gcacagaaac aagatattag
    25741 aaactttgta tcatatccag catacaaatt atacttcgaa ttatgaagag gctttttatt
    25801 attttaagcg aagagagcgt cacaggagta tcatattttt ctttacagat tttgattctt
    25861 ttgaagaagc tgaaatgatg tttaaggtac tttctgttat tacaagaaat aatttggttg
    25921 ttattgctct gatgaaagac gagcaagtgg aacaaattgc ttcttctgat ataaaaagcg
    25981 aattagacct ttataacaaa ggcgttgccc ttgaattaat ggatgaaaga aagaaaatta
    26041 tccgtcttct caatcaaaaa ggggtatttt gtttagaaag cacccctgaa aaaatggaat
    26101 tagctacggt taataaatat attcagataa aaaataaact cattttttga ttagataagg
    26161 aagcgagaag tacagtataa gaagaatgcc agcaaatgca gcaaagatta attttatgtc
    26221 ttcatctatt tccaatgggg tgaaaaatcc ttcaataata gctgctataa caagcaaaac
    26281 aacgacacca atcagtaaag agactgaacg tttagcctga gcaatcaagg cgtgtttgcg
    26341 ggagtattct ccaggaataa ggagactttt tccaatcata attcctgctg caccagaaat
    26401 aaatattgca gtcagttcaa taacaccgtg aggcagaata agggaccaaa aacggacagg
    26461 gtcattgtaa agatatacca gaccagtcaa agcgcccagc aatgccccat tagtaaataa
    26521 aatataaatg gtgccaattc caaagctgat tccgtaggca aaagctttga aagccaccga
    26581 aatattattg gtcataattt tactggatat aagaggatag tcccactggc cggatccaag
    26641 ccgttgacca tctgaaatgg cttctatcat cgtttcattt aaaaaaaatc tggccagaga
    26701 atcatcataa aaaaccataa taaggcttag taagaagcca aaaaagaata taccaaagga
    26761 aataagaaca taagctttta attcttttaa tgcattgggg aagtcgtaaa aaaaagaatg
    26821 aaagctttct ttaaaagaag atttccttac aagataaaca tggttgtggc attttccaat
    26881 taaagagttt aaataagtta cagtattgct attgggataa tgggttctgg cataggccaa
    26941 atgatggctg gtcagtttat aagtatgcaa aaattcttta atttctttag aatttaagct
    27001 tgttattcct tttttatcaa tgtgagcaga caaacattct aatgttttcc aagtagctga
    27061 ctttgattta ataaactgtt cttctttcaa ttgtctcacc tcatgaatat tttaacaaaa
    27121 atttaaagga atgtatacag aaaggataag taattggagt gattttaatg aaaattaaaa
    27181 ttattacccc ggaaaatatt gaggtagagt attccctggc taatttaggt tccagggcag
    27241 ctgcttttat tattgatagt ataattgaag gtttgatatt ttcaatatta ctgctcattc
    27301 ttttttgtat ctataagatt aacgagcctt tttgggaagc attttacggt tggatctggg
    27361 ggatttttac aattgtttct tttgtaattt atcaggggta ttttattttt tcagaaatga
    27421 atatgaatgg gaaaaccata ggaaaaaggc ttatgagaat ccgtaccatc agaagaaatg
    27481 gacagagttt gacctttaag cattctttaa tccggaattt attcaggact ttcatagatg
    27541 tttttggaat cggtgttgta ttgatgtttt taaataaaga gcacaaaaga ttaggcgata
    27601 tggtagcttc aaccattgtt gttgcagaag aagacaaaac ccggccggtt tccctggaaa
    27661 gccttttaaa atccaatcag ggaacttata attattattt atctcagcat gagcaggatt
    27721 tattaagaga gtattatagc cgaagatact atattgaaga tagtgaagag ttaaggaaga
    27781 ggatgagatt gtattttgca gagaaatttg aaagagaagg gttactggaa gaatttaaag
    27841 attttattaa taatttataa aacaatacaa gttaaaaatt gatatcactt atatataaga
    27901 gtaagaggac tttggaggtc ctttttttat taatagaatt aaaaattgta ttgacaactc
    27961 taaatcactt atagtatgat taaatagaca gacagtcggt ctataaattg gaggtgtaat
    28021 tatgcgggtc gtaaaaacgg ctgacgaacg cagaaatgaa atcctggatg cagctgaaaa
    28081 gctttttata caaaaaggtt ttgatgcgac aagtaccaat gatattttag aggaagtcgg
    28141 aattgccagg ggaactttat attatcattt tgaatctaag gaagctattt tagacgccct
    28201 gattgaacgc tataataaaa aagttttaga caaggcaaaa gaaattgcaa gagacaaatc
    28261 cttatccatt attgagcggc ttatgggcgc agttatggca ctgaatatta aacaaaaaag
    28321 tgaaagagaa atcattgaag agattcacaa gcctcaaaat gctttgatgc atcaaaaagt
    28381 gcaaagatta atactgacag aggttacgcc tattttggca ggaattattg aggatggtat
    28441 cagagaaggc ttatttaata cgccatatcc ttatgaaacc atagaaatgc ttattgctta
    28501 taccaatacg gtttttgatg atgaaattgt taaaatgact gaggacgaaa agaagagaag
    28561 gatagcagcc tttattttta atctggaaag attattagca gcaccgaaag gaacttttat
    28621 ttctattatg aaggcttttg aggatagtga aaaaaatgaa taaggaaaaa gttttattta
    28681 gaaaatttat gattttatgg ctgggacaat ttatatcggc cattggaaca ggacttactt
    28741 cttttgggct aggcgtatat gtttatgagt taacaaagaa agcctctctc gtagcgctga
    28801 tatctttgtt tgcttttttg ccttctatac ttttaggtcc cctggcaggc gttttggcgg
    28861 accggtatga cagaagactt ttaatgataa ttggcgacag cttttctgct ttaggtcctt
    28921 tgtttatttt gttctcgatt ctttttggta agattgaact ttggcaaatt tttttagggg
    28981 ttttaataag ttctgttttt tcatcccttt tagagcctgc ttttaaggca acaattacag
    29041 atttactgga tgaaaatcag tattcaaagg ccagcggtct taatcaattg gctggctcgg
    29101 caaaatatct tatatcacct cttctggcag ggatgctcct tgtgatttca gatattgaat
    29161 tattgctgat gattgatatt tttactattg tcattacagt ttgcacaaca attatcgtca
    29221 ggaaggggat tgctgtaaaa gtacaagaag agcgtcattc atttatagca gactttaagg
    29281 aaggctggaa tgaaatatct aaaaaggaag gcgttaaagt gttggttgca gtgacatcct
    29341 taatgacttt ttacgtagga attatacaga ctttgaccac acctttaata cttgcttttt
    29401 ctaatagcgc tgttttaggt atgatagaaa ctctttctgc tttaggtatg ctctttacta
    29461 gttttgtgat tggaatcatc agtataaaat caggttattt aaaaatgctt tcgaaatctt
    29521 tgttttttgc tggtatttgt atgtttttct ttggacttag agaaaatatt tttttaattt
    29581 gtataagcgg ttttgtgttt ttcgcaatgt tgccctttgc caatacgagt ttagattatc
    29641 tcatcaggat taatatcaat aattttgtcc agggaagagt atggggactg attggtttaa
    29701 tatctcagtc aggttatgtg atcgcttatg cagtatctgg attattggca gattatcttt
    29761 ttacgccatt atttattgaa ggaggtctgt tttataatag tcttgggaaa atcattggga
    29821 caggtacagg cagagggaca ggctttttaa ttatgattac cgggctcttg ttatctgtta
    29881 ctgcaatctt tttatatcat ataaaatcca ttcagaaact tgaaaaaaga ggtgaaatat
    29941 gtatttaaaa ataatccgaa acgatattac taagagtaaa ctgataaccg taataactgt
    30001 tttatttatt tcagcagcag ccttgttaat ttccttagtg gctgttttag ctgtgaatct
    30061 tttgggcgct gtcgataccc ttatgacaca agggaaggca cctcattttt tacaaatgca
    30121 ttcaggtgat attgatttga aaagactgga gcagtttgct cgggaaaatg ataaagttga
    30181 tgaatttcag gtcctggaat ttttgaatct ggatggggaa aagattcaat ttgaagatat
    30241 gactttaaga gatagtgttc aggataacgg atttacgatt caaagtaaaa agtttgacta
    30301 tcttatagac cttgagggaa aggttattca gccagttaaa ggggagcttt atgttcctgt
    30361 atcttatatg aaggaaaata taacaaagct tggagagacc gtaaagatat gtgggaaaga
    30421 atttatagtt gcaggatttt tgagggattc acagatgaac tcttccctgt gcctttcgaa
    30481 aagatttctg attagtgaag aagactatca ggaaattaaa aatgacggga atgtagaata
    30541 tttaatcgag tttagattaa aggacttgtc agatcttgct gaatttgaaa atgaatatat
    30601 agcagcagga cttgagtcca atggaccagt cattacatac ccggttttta aaataattaa
    30661 tggtctttct gacgggatta tgattgcttt gatattatta gtgagtattc tggtcatttt
    30721 aattgccttt atgtgtatac attttaccct tcttgcaaag attgaggaag actacaggga
    30781 aattggtgtt atgaaggcga taggtttata tgtttcggat ataaagaaat ttttcctggc
    30841 taaatacata cttatatctg ctgcaggttg tttagctggt ttttttcttt cttttttctt
    30901 cagagagcct ctgctttata atatacgcct ttatatggga gaaagcccta aaagccattt
    30961 atctttatat gttgctatta taggtgtcgt ctttgttttt tatactattc ttacttatgt
    31021 aaaaaaaatc ttaaatcgtt ttaaaagaat ttcgccttct gacgcaatag gttttggcgt
    31081 ttctaaagaa aagacaaagt atgtgaaata ctttttatta agccgtagta aattgcctga
    31141 gattaatctc tttttaggaa taaatgacgt gctttcaaag aaaaagcttt atttaacgat
    31201 gtttatggtc tttgttattt caaattttat tatgattgtt cctcaaaatc tacatcagac
    31261 ggtttcgtca aaaaatttta taaaatatat gggtatagga gcatctgata ttcgtataga
    31321 tattcagcag cttgaggata ttcatgcaaa aagcatggag atcataaaaa ggataaaaga
    31381 agaccaaacg attaaaaaat atgctgtttt cacaacaaag gcatataggc ttatccattc
    31441 agaagggcaa gaagaaattt taaaagttga gttaggtgat catacattat tccctataga
    31501 gtatgccaaa ggccatccgc caatgactga aaatgaaatc gccctttctg ttattaaagc
    31561 agaggagcta aataaaaatc tgggagacag cataagctta cttattgatg gaaacgaaaa
    31621 aagttttgaa gtatgcggta tttattcaga tgtgaccaat ggcgggaaaa ctgctaaagc
    31681 gaattttagg gatgataata cggataccat gtgggtcatt attcagttag agctttcaga
    31741 taaaactttg ctctctgaaa agacccagga atatgcccaa gcttttcctt ttgcaaaaat
    31801 ttcaggtatt gatgcgtata tagggcaaac ttttggacag ttaattcatt cagtaagaat
    31861 agcttctttg gtttccgttg gagtcgctgc ctttttatcc atacttatca ctttattgtt
    31921 tatgaaaatg ttgataacaa aagacagata tgccattgct cttatgaaat ctcttggttt
    31981 taacaataca gacattaaat ttcagtatct tttccgtagt ttatttgttc tgtttatatc
    32041 gattattatt ggtacaattc ttgcaaacac tttgggagag ctaatggctg gagcagccct
    32101 atcctatttt ggagcttctt attttaaatt tgaaataaat tacctaaaag catatttact
    32161 ctatccctta ttattgatgt gttgtgtaat tactggtaca atcattggca gtttaagggc
    32221 aggagaaata gaaatatctg agagtataaa ggagtagccg cctatgaaaa aaataattgt
    32281 tgctgaaaaa gttgtaaagt catttggagc agatggtcaa tgccggaagg ttcttgataa
    32341 tgtctctctt acggtttatg aaggtcagtt tatttctgtg atgggtcctt ccggttcagg
    32401 caaatccact ttaatgtttt gtcttagcgg aatggataaa atagatagcg gcagagtaat
    32461 atttgacgga aaagtcttat cagacttaaa agaaaaagaa tttgctgata tccgaagaaa
    32521 tgagatgggc tttgttttcc agcagcccac cttactaaaa aatcttaata tccttgataa
    32581 cattattctt tcatctatga aagaccttaa taaaaataag gagcaaatca taaaaaaggc
    32641 tcaaaagctt atgaaaaagt tagggattag tgaattagaa aaacgggata ttacacaggc
    32701 ttcaggagga cagcttcaaa gggcagggat ttgcagagct ttaatgaata atcctaaaat
    32761 tatctttgca gatgaaccta caggagcact taattcaaaa tcagcagagg aaattatgga
    32821 tatctttgat aatataaaca aagaaggtac gacaattatg cttgtcactc atgatgcaaa
    32881 agttgcagca aggacggaaa ggattttatt tataaaggac ggaaagattg tcagtgagtt
    32941 aaaacttcca aaatacgaag gtaaaaatat ggaggaaagg ttagaaaaag taacggatgc
    33001 aatgttagaa ttgggcatat aattgatatc aatcgccaaa gcgtagctta ttctggttta
    33061 tccaggataa gctatctttt atttataaaa gttgaattga caaagaataa aaattgtagg
    33121 atgattaaaa ggaaatattt ataagttttg tatggttttt ttatctaaga atgtgtttaa
    33181 aaaaaagagg tgtcatcatg atagaagcaa atgagctatg taaaattttt acccgctatg
    33241 tgaaagatga agaagttaca aacaagagaa aaaaaataaa aacaaaaaaa gaagattttt
    33301 ttgcagttga tcatatcagc tttaaagccc gacagggaga aatatatggt attttaggtc
    33361 ctaacggggc aggtaagacc actttactta gaatgctggg aggtattttg acgcctacat
    33421 caggggaaat aaagctggct ggttatgatt ataacctgga taggaaaaag gtaaaaagga
    33481 aaattggtta tttatcgggt aataccaaat tatatggaag aattacgccc agagaacttt
    33541 ttaatatttt tggccgttta tatgaaatga gcaaaaatga gattgaagaa atgactgaaa
    33601 aaatcatcga aattatggat atgaaagatt ttgcagataa ccggattgag catctttcaa
    33661 caggacaaat gcagcggact tctattgcca gatgtctttt acattcgccg gaagtttata
    33721 tttttgatga acctacctta ggtctggatg ttatcagcag tcatgccatt atagaattta
    33781 tgaaaagtga aagagaaaga ggaaaaaccg tattatattc tacgcattac atggaagaag
    33841 cagaaagttt gtgtgataga attttgatga ttcacaaggg taagttaatt gcagaaggaa
    33901 caccagaaag cctgaaaaaa gaaagccatg aaaacaatct gagagatgta tttatcaggc
    33961 tggtaaagga aagaggggaa tggacatgag aaaaaatata gtattatcga tatttcaaaa
    34021 ggaaatgctg gaccttttgc gagataaaaa aaccctcttt atgatgatta ttttacccat
    34081 tattatgtac ccagttttat ttatcttgtt ttcttttctg atgataatgg gaataagcag
    34141 tttacaggaa aaagaattac cgattgcctt taataagtca cctgatgcag ccattattgc
    34201 aaagattttg ggaggtaaag aaaagacagg gaaattaaaa attgttgaag tggaagatta
    34261 tcaaaaagct ttagaagaaa gagaaattac ggcttatatt gaaattgaag aagaagacgg
    34321 acaaattcaa tataaaatat atatgaattc ttctgttgaa gattctaatg aatctacggg
    34381 aagaattaaa gatttactgg aagagtataa ggatgaactt gtaaaagaaa aaattgaagc
    34441 ggaaggtttg gatgttgatc tgatacttga acccattgtg tatgaaactg tggatttggc
    34501 taaaaagaca gaaaaagccg gctacctgat gggacaaatt cttccggtga tatttattat
    34561 aggaatttta ttaggtgctg tatatcctgc taccgatgtt atagctggag aaaaggaaag
    34621 aggaaccctt gaaacccttt taactttgcc agtatccaat ggagaactgg ttatgggtaa
    34681 atatctggct gtatctctta gtgcaattat ttcagcaatt ttaagcgttt tgtcgatttc
    34741 tcttagttta ctatttttaa tgggaaattt taaagaagca ggagcaattg atttagaagg
    34801 cattaattat aatgagttaa tttttccttt aatcattacc ttaatttgtg tatgtctgtt
    34861 tgctatggtt gtatctgcca tcagtatgtg tgtttgttct tttgctaaga attataaaga
    34921 ggcacaaaat tatatttcac ccattatgct tattcttatg attccttcat atgtatctgt
    34981 aatgccgaat atggaactta acggggttaa tgctgctata ccggttgtca atatatccct
    35041 tttaataaaa aatgtattat catttaatta taatttagct gccatttcgg ttgtattagt
    35101 aaccaatatt gcgtttgtac tgctttcaat ttattttctt tcaaaaatat ttaaatccga
    35161 agatatcttg tttggggata aaagagaatt ctcttttctc gaaaaacgtt ccaatattaa
    35221 aaaaggcagt ttgcctactt tgagtgatgg tttaatttta tatgccatag ctttgctggt
    35281 gttgatttat ttaggtggtt ttttacaggt taaataccag attttaggaa tggccttgac
    35341 gcagtttatg attattgctt ttcctttatt ttttggatat tatataaaaa cagattttgc
    35401 aaagactttt tcaataagag ttccgcggct aaaagatttc ttaggtggtt tcattctgtg
    35461 gataggcgta agtattgctt ctttgttact ggctcagttt attttgtatt tgttcccgga
    35521 aaatatggaa gttgctgaag cacttagtga gtttattaca ggagattatc cttttatttt
    35581 gtctttactt gtttttgccc ttttgcctgc aatttgtgaa gaagctttat tcagaggctt
    35641 tctgatgaca gcttttaaag acaagaaagc cattttaagg gctattattt taagtagtct
    35701 tttgtttggt gtaatgcata ttgactttat caggataatt cctacaacca tccttggtct
    35761 tgccctgggc tatgcggtat attgtacagg ttctattttt attccaatgc tgatgcattt
    35821 tttaaacaat ggtattgtcc ttttttcagg atattttcct gagcatatca tcagcaaagc
    35881 ctattcttgt ttagaaatag atttttctaa tctggatttt ggtcatctta tttcactgat
    35941 tgtattgagt attgtcttta caatcattgg ccttgtatta ttaaaaaaac caaaaaatga
    36001 aaaagtacca ggttcttaaa cttttttctg acttagaaaa agattcattg aagacaagag
    36061 caaataaaaa tgtcatatgg taaaatataa tgattcaggt taatggtcag tcggaaagag
    36121 taaaaatgtt taaggagagg cgtttatgaa tcttcaatac ttaagatatg ctttggaagt
    36181 agaaaaaact ggttctatca gtaaagcagc cgagaattta ttgatgagcc agcctaattt
    36241 aagccgtgcg attaaagaac tggaagagtc attgggaatt actattttta aacggacttc
    36301 aaagggagta tcggcgacct atcaggggga agaatttttg tctcatgcca aaagaatact
    36361 tagccaggtg gatgaagttg aagccatgta taaaaacaga aaagcaaata aacagagatt
    36421 tttgatttct gttccccggg ccagttacat cgcctgtgct tttataaatt ttataaataa
    36481 aattgatatc aatcaggata cagaaatata ttataaagaa accaattcct taagggctat
    36541 caataatatc ctgcaagctg attataaatt ggggatcata cgttatcaaa caagttttga
    36601 gcaatatttt aattctatgc tttatgagca tggtttagct tcaagagaaa tactaagttt
    36661 taatagtgtt ttacttttat caaaggaaga tccccttgcc cgaaaggata agatagagcc
    36721 ttcagattta agaggttata tcgagctggc ttatccggat ccttatgtgc cttctttacc
    36781 tttgaccgat gcaagaaagg cagagcattc aaaatgtgtt gaaaagcgga tttatatttt
    36841 tgaaagagca agtaaatttg acttattaaa aaatataaaa aatagtttca tgtgggtttc
    36901 gcccatgcct caggaacttt tggacttgca tcagttagtt caaaaaccat gcaagtttaa
    36961 taaaaaaatt tataaagatg tcttaattta tagaaaagat tattatcttt cagatttgga
    37021 tcaagctttt attgacgaaa tagataaaat gaaagaaaaa ataaaataat ttttgaaatt
    37081 ttttataaga gtcagtaaga catatcattt ttgatatggc tttatttatt atttatatta
    37141 cacagataag taaaagaatg ttaatatatt aacagagatg aatgttaaat aattaacata
    37201 agaaaaaaat tcatttgatt atagatcaag ctttatcaaa atgaaagaag gaggaattag
    37261 tatggataaa aataagactt atacagtagt ggacagcgtt gaaaaattag atcaggcaat
    37321 tcagaaagtc agaaaggcac aagaggtatt tgcaacctat agtcaggaac aagtagacaa
    37381 aattttcttt gctgctgcat ctgcagcaaa taaagaaaga atccgactgg caaaaatggc
    37441 tgttgaagaa accggcatgg gtgttgtaga agacaaagtg attaaaaacc attatgcatc
    37501 tgagtacatt tataatgctt ataaggatgt caaaacttgt ggtataattg aagaagacaa
    37561 ggcatatggc attaaaaaaa tagcagaacc tattggtgtt gttgcagcgg ttattccaac
    37621 gaccaatcca acatccacag cgatatttaa aacacttata gcacttaaaa caagaaatgg
    37681 tattattata agccctcatc caagagcaaa aaagtgtaca gtagaggctg ctaaagttgt
    37741 actggaagct gctgttgctg caggagctcc ggaaggtatt attgactgga tagatgtacc
    37801 atccttagaa atgaccaatt atcttatgag tgaagcagat attattcttg caacaggtgg
    37861 acctggtatg gtgaaggctg cttattccag cggtaaacct gcaattgggg ttggagcagg
    37921 gaatacacca gccattattg atgattctgc agacatttta atggcagtta attcaattat
    37981 tcattccaag acctttgata atggtatgat ttgtgcatct gaacaggcag tgattgttct
    38041 aaatggtgtt tataataagg tgaaaaaaga atttgccaga aggggttgtt attttcttaa
    38101 caaagatgaa attgataaag ttcgaaaaac cattattatc aatggggctt taaatgctaa
    38161 gatagttgga cagacagcat caaccattgc ccaattggca gggattgaag ttcctgaaaa
    38221 cacaaagatt ttaattggtg aagtagaaag tgttgatatt tccgaagaat ttgctcatga
    38281 aaagctctct ccagttttag caatgtataa ggctaaagac tttaaggatg ctctggacaa
    38341 ggctgaacaa ttaattgctg acggaggata tggccatacg tctgccttat atattgatac
    38401 agtaaacgaa aaagaaaaat taaaagaatt tgaagcccgt atgaaaactt gtcgtattct
    38461 gattaataca ccatcttccc agggtggtat aggagacatt tataatttta aactggctcc
    38521 ttctttaact ctcgggtgcg gttcctgggg cggaaattct gtttctgaaa acgttggggt
    38581 aaaacacctt ttgaatatta aaacggttgc tgaaaggaga gaaaatatgc tttggtttag
    38641 agcacctgag aaggtttata ttaagaaagg atgcctgcct gtagcccttg aagaacttaa
    38701 gaatgtaata ggcaaaaaga aagcctttat tgtaaccgat acattccttt atcaaaatgg
    38761 ttatacaaaa gttataaccg ataaactgga tgaaatgggc attaagcata cgaccttcta
    38821 cgatgtagag ccagatccat ctcttgcatc tgctaaagaa ggtgcaaaac agatggccgc
    38881 ctttgaacct gactgcatta ttgctgtggg tggaggttct gcaatggatg ctgctaaaat
    38941 tatgtgggta atgtatgaac atccagaagt tgactttatg gacatggcta tgagatttat
    39001 ggatatccgc aaacgggtat atacattccc taaaatgggc gaaaaagcat attttatagc
    39061 gattccaact actgccggaa caggttctga agtgacacct tttgcagtta ttaccgatga
    39121 agaaacaggt attaaatatc cgcttgctga ctatgaatta atgcctgata tggccattgt
    39181 tgacccggat atgatgatga atgcacctaa gggtctgaca gctgcatcgg gtattgatgc
    39241 tttaacccat tccctggaag caattgcttc tatgctggct acagattata cagatagctt
    39301 agctttaaga gcgataaaaa tcatttttga atacttgcca agagcttatg acaatggacc
    39361 gaatgatcca gttgccagag aaaagatggc taatgcggcg actatggccg gaatggcatt
    39421 tgcaaatgca ttccttggtg tttgccactc tatggcacat aaattaggtg ctttttataa
    39481 attacctcat ggtgtagcca atgcccttat gattaatgaa gtattacgtt ttaatgctga
    39541 agaagtacct gttaagatgg gtacattccc tcaatatgct tatcctaaga caatagaaaa
    39601 atatgctgaa gtagctgatt acttgaatct tggtggaaag agcaatgaag aaaaattaga
    39661 aaacttaatt aaggcaattg aggaacttaa agaaaaagtt ggcatcaaaa agaccattaa
    39721 agattatggt atcgatgaaa aagttttcct tgaaaactta gatgaaatga cagagcaagc
    39781 ctttgatgat caatgcacag gtgcaaatcc aagatatcct ttattgagtg aaatcaaaca
    39841 aatgtattta aacgcttatt atggtagata agggggaaga agcaatgaag ttagatacaa
    39901 ttcttgccgt aagaaaatcc aaaacaattt accgggacgg agataaagtt ataaaagttt
    39961 ttgacaagga tttttcaaag gcagatattc ttaatgaagc attaaatcaa gccagaattg
    40021 aagaaacagg ccttaatatc cctaaaatta ttgaggttac tacagttgat ggtaaatggg
    40081 ctattgtcag tgaatatatt gaaggaaaga ccttagaagt attaatgaaa gaaaatccgg
    40141 ataaattcga tgtgtacctg gaaatgtttg tggatttaca aatagaagtt cattccaaaa
    40201 agtctccttt attaaataaa cttaaggata aaatgataag aaaaattaat gaaactgatt
    40261 tagatgagca tattcgctat gaattacgaa cgagactgga aggaatgccc aaacataaga
    40321 aggtatgtca tggagacttt aatcccagca atattatcat aaagccagat ggaacccctt
    40381 atattttaga ctggtcccat gttactcagg gaaatgcttc tgcggatgtt gcaagaactt
    40441 atttgttgtt ttctcttgcc ggagaatctg atactgcaga gaaatattta gatttattct
    40501 gtaagaagac caatacctct aagagatatg ttcaaggatg gatgcctatc gttgcagcat
    40561 cccaactggt aaaaggtaaa ccggaagaaa gagaattttt aagacgctgg gctacagttg
    40621 ttgaatatga ataaaatgcg atggatgtaa gtgacatttt tagagttaaa ggtaggaaaa
    40681 attcctacct ttaatctttt atttgactta atattttgga taatttaaga tgaaataagg
    40741 gtattttaaa tgcttaaact ttttaagatt attcggggga ttcgtcatga aggaaaaaac
    40801 aaaaaaaatc agatctttta gatttaaatg gtatgagttt tttctgatat atggtttgat
    40861 taattattat ttttttacgg caataaattc ttctttgttt tctttgcaaa gagagatgat
    40921 aaattgttac tcagataatt tagagattga tggaacagac tttgttcctt tttttaattt
    40981 tatgtcattt tcttttctta cgggtttatg gataatcgtt gcaattataa tcattatgat
    41041 ttctattata tccctgctca tttttagatt tatatatttc agatcagtaa ttaatcagga
    41101 ggaaatcaat aagttattaa aatatatgaa aaatacaata atccttttaa ttatggttaa
    41161 tatagcagct tcaacgattt tgttttatat agaatatcat tcttttaact atataataat
    41221 tcacttattg ggctttttag gagtatatgt tacaatacct ttatttactt atttattgat
    41281 atatgttaag ttaaaaaaga tacataatat aagttaggaa tctttaatac ttgttgtaag
    41341 caggtgagaa gaatgtatgc caataatcct tcgtggaaag tattgcaaaa ctttttgcca
    41401 gaaaaaaacc gaataacaga gcgtataaca cccgaggaaa aatatataaa aataaaagat
    41461 atggaaattc atttggacga atacaataat aattctgata caagtatcat tatgtttcac
    41521 ggagtcgggg gtaatggccg cttactgtct tttattgctg ttcccttatt taaggctggt
    41581 tataatgtct tatgtcctga tttgccgggg tatggttaca gcaggtataa taagttactt
    41641 acatattcag attggattga tacaggggct gctatcgtgg aaagggaatt ggctaaatca
    41701 aagaaggttt ttgtgatggg cttaagtgca ggtggaatgt tggcttataa tgttgtttgt
    41761 aaaaatcagg aggttcaggg tcttattgtt acaaatttgc tggataaccg tgaagaagaa
    41821 gtcaggatac attctgctaa aaataagtta caggctaagt atgggattca gttattaaat
    41881 cattttccag gttttttaaa gacgattaaa ctccccatcc gtatggttac caatatgaat
    41941 gccattgtga atcataaaga agttttaaag gttatattaa aagataagct gggggcagga
    42001 aataaggtaa gtcttaattt tttattgtct atgatgaaca gtattccttt aattgaacca
    42061 gaagatttta aaaaagtacc tgtccttatg gtacatccag ggaatgattt atggacaccc
    42121 gtaagaatca gtgatttatt ttttaataga atagcatcca gtaaatatag agtcatttta
    42181 aaaaatgccg gacattttcc tgtagaagaa gaagcattgg tccagatgga aagagaaatt
    42241 gttcatttta ttaggagttt gttacaaaaa tagaatagaa tgaataaaaa aggaactaat
    42301 aatataaagg cttcgtctaa agagtaagat actcagagga ggaatagtga tgttcaaata
    42361 taaaaagttt tgcttattac cagccttatt aatttttgtt tttttcttta gtgcttgttc
    42421 aaagtttatt gcaggagaaa agcaaaatga taagactgaa attccacaaa agacaaatga
    42481 agtgataacg gaaaataaag aaaaaaagaa attaacaaga gaagaagtta tagccataga
    42541 gaaggcgttt aatgaaagga tttttcagtt agaaaaagta gaagacagta atgaaattct
    42601 gaattttaaa agcaaggaag aactgatgga ctatgttaag gaagtagctg gtgaagaatt
    42661 agcaacttta tttatagaaa acttttatga agagatagat ggaaaattat acgttattcc
    42721 taagggaaca cctccaaggc ttatttatga ctctccattt gaaataaaca agatagatga
    42781 taatacttat gaaattgttc aggacgaaga aaatgaaatg tatggaccct acagattgat
    42841 cattgagttt aaatatatag atggtaagtg gaaaatgaca gacagaaaag cagaaggcag
    42901 ggaaaatgca aaccaggatg acagtaactt ttcaaaggag gaaaggatag attcagaaac
    42961 ggctaaggca atcattagtg atacagccaa tcgtcttatt tatgccttga gtataaaaga
    43021 tgtcaatacc atagccgagt atgtgcatcc ggaaaaaggg gtaagattta caccttatac
    43081 ttttgtttca ttagaagagg atttggtttt tagtcaggaa gaaatcaaaa atttctttga
    43141 agataaaaat atatatacct ggggttatta tgacggcagt ggagagaaaa ttaatttaac
    43201 gcccgcagaa tattatgata gctttgtgta ttcagcagat tttataaatg ctccggagat
    43261 aggctataat gaagtattaa gtcaagggaa tatgcttgaa aatcagtttg aggtttacca
    43321 caatcccatc atagtagaat attattttcc tggttttaat cctgaatatg agggattgga
    43381 ctggcaaagc ctgaggcttg tttttgagga atatgaaggc acttggaagc tgtcaggaat
    43441 tatacacaat caatggacca tttaaaagca gataagaatc attttaatga ttgaatggta
    43501 taaaaggtgg aaccaattat ttttttcatc gtctaagaga tgaaaaaaat aataaaagga
    43561 agtgatcaaa tgaaaattcg ttggtatggt aaaaagataa cagcaggatt attattaacc
    43621 gttttaatgg ttcctacttt ttcacaaggt gcagatgtca atttagtatc tgaaaatata
    43681 gaaattattc ctgtcaggta tgaatacaag cattgggcac agccggctgt agaaatttta
    43741 agtcaaaatt atgatattgg gcagtttttt gaaggcaaac atcttaatga ctttattagt
    43801 gcagaagatt tccaaatact gattcaaaaa actattgata aagattataa tggagtacct
    43861 gacgctctca ccagggaagc agttgtgtat gaattggtta aaatttgggc taaggaaagc
    43921 catcaggatt taaatactat tggggttatc gaaatgctga tttattcgga tacagacagg
    43981 atttctcctg attataatca tgccattact atagcctata tgaaaaatat tgcaaaaggc
    44041 aaaggctcag ggatttttga tcccaaggca aaggttagtt atggagagtt agcagccctt
    44101 attaataata cgattgcagc gatagaaaag gaatccagtc agcaggatat agccagtgaa
    44161 aaatttgaaa ccaggggaag ttatgatatt aaagatgata aagttatact ggattttgag
    44221 ttaatgaatc attacaacaa ccctaaagat ttaatgttta gttcaggtca gcaatttgaa
    44281 atttctataa tagatgaaga aggtaatgaa gtttaccgtt attccgatgg taagttcttt
    44341 accatggccc ttgtttataa aaccatcaat ccgggagaat ctttaaaatg gcaggatgtc
    44401 tgggatatga ccaataaaga cggtgaaaaa gttaaaacgg gcaaatatac agcaaggatt
    44461 agtatcttat gccaggaaat taatagtgat gaagccgctt tagaggagag tgtcagtgcc
    44521 ttagacttgt cttctgtttt agagaccgaa aaagagaaaa agagtgaatt tacaactatt
    44581 attgaaatag atttataaga tgcagaaata aaataaataa tctttcttag atcattatag
    44641 cctgtctggc atgttcagac aggctttgtt attgtattat aaaagatttc aagttaaaat
    44701 atagatatga aaaggcaaac tatataaatt atttaaaaat catagtgata gtggaaaaga
    44761 catgatataa tattattgct gattaagaca cgtaagtaaa aaataaaaag gttaggtcga
    44821 aaagatagaa atcagaatta aaaataaagt gatgattaga gcagaagaaa aatttgtaat
    44881 aataagttaa atgtaaggag atatttatga agttaagata tgtaatggac atgactgaag
    44941 gtaatgaggt atccttgatg attaagtttt cgttacccat gctgattggt aatatatttc
    45001 aacagtttta taatatggtt gattctatta ttgtgggcaa atttgttaat gccaatgcct
    45061 tagcatctat tggcgtgacc agttccataa gttttttatt tttttcttta tgtttgggct
    45121 tggcagttgg agtaggcatt ataatcgcac aatattttgg agcaagagat gataagaatg
    45181 taaagagtgc tattgctaat tccatttatg tcataggaag cacatccatt tttataagta
    45241 ttttggggat atttttagcc agaccggtcc tggaatttat gaaaacacct gttgaaatac
    45301 tggacgatgc ggtgctgtac ttgcaaatta tagcaggagg aaccctggct gtaggtgcat
    45361 ataatgccat gtcttttatt ctcagagcat taggagattc aaagacgcca ctgtttttcc
    45421 tcattatagc ttctgttata aatgtaattt tagacttggt ttttgtgctt gtttttggct
    45481 ggggagtagc aggggcagct attgctacaa tcatttctca aattgctgct gctacaggtt
    45541 gcatcgtctt tgctgttaag acagaatatt attttagact gaaaaaagag gaattaaaag
    45601 ttaataagaa tattattaaa gaaaccatta aagtggggct tccagtagca atacaaaatt
    45661 caacgattgc cttttcatta ttagcacttc aaaatattgt aaatcgattt ggaagagttg
    45721 ccgttgcatc ctatacggcg gttatgagaa tagaacaatt aatacagcag cctttcaatt
    45781 ccctgggagc agctatgtct acattttctg ctcaaaacat gggagctaat aagattgacc
    45841 gtattaaaaa aggctatcac aaaatgattt ttattattgc tgcctttgct ctgatggctt
    45901 tttcaggagc tcagtttggc ggacgttata ttatgagaat gtttgttaat gaagaagatg
    45961 ttattgccct cggggcaaaa ggaatcagga tttcgagttg tttttatttc tttttgggta
    46021 tgatttatat caccagaggt ttattaaatg gtgctggaga cacggtttat gccatgatta
    46081 acggctttgt tgaagtgata ggaagagtag gtttttcaag tattctttac tttttccctg
    46141 tttttggggt tatgagtatc tggattacga caggtctgac ctggtttatt acagcagggg
    46201 ccagtgtggt acggtatata cagggaaaat ggaaaacaaa atctatcgta aaagtgaatg
    46261 aacagtaatt tatcttgaac ccctatacat tagtataggg actttttgtc cgtggactat
    46321 ttaatttttt atactacttg ctaagaatga agcaaggtgg cttgtataat cggtcaatac
    46381 ttcagtatgt ttatctggat ataaggttta aatcgtttac aaaagtaaaa aaattataaa
    46441 taattatttg acttgtcatc ggagtatggt ataattgaaa aaccgtacag aaaaaactaa
    46501 gaatattaca tggagagaaa tgatggatat tctgataaga aatgaagtaa aagaggatta
    46561 taaacaagta gaagaattaa cccgggaagc attttggaat ttatatttac ctggctgcag
    46621 tgaacactat ttggttcatg ttctaagaaa acatccggat tttattgcag aacttgattt
    46681 cgttgcagta ttggaaggaa aaattgtggg gaatattatg tttactcaat cacgtttaat
    46741 taatgaagaa gatgatagta ttgctgtatt aacattagga ccagttagtg tattgcctga
    46801 atatcaaaaa aagggaattg gctctaaatt aatacaacat gctgtaaaga aggctgctga
    46861 aaaaggttat aaagtgattg ttctggaagg ctatcctcat aattattgta aacatggttt
    46921 tctaggctct aaaagcctga atgtcagtga taagaatggc agatatccat atagtttatt
    46981 ggttcttgaa ttagaaaaag gattattaaa gaatcattcc tggaaatatc atcccagtga
    47041 tgcttatgat attgatgaaa acgaagccaa tgaatttgat aagatgtttc cacctaagaa
    47101 gaaagcatac cagccatccc aggaagaatt taatattgca tgtaatgcat atattgaaga
    47161 cttatagaaa atctataagt cttttttata ttttacagta tataaaaata atttttattt
    47221 tgtaataaaa catgctataa taaatgtata aatttacctt gttcgagaat tagagaagaa
    47281 tatggaaaga atttatggga tctaatcata tttttatttt tcaggaggta taaataatga
    47341 ggagaaatta tgagcaagtg tgttccaata taaaatcgct tttttatttt ggtttatttt
    47401 tgttgataag tattggactg actgcttgtg ttcagcaagg ggcttctgac ttaacttcag
    47461 atgaagttgt tcattctgaa atagaagttg tagaaaaaac tgagatggca aaagaaaaaa
    47521 cagaatatct tgataagaat aaagcagaaa aaagcaatga agaaataaat cattttgaaa
    47581 cgaatcctga ggattttttt aatttacagg aaaatatcat cagaaacgtt cttgaagtaa
    47641 ataaagaata tgatgatgaa gtttttgagt taattgtgag aggaaaatat gatttgaaca
    47701 aagatggcag tcttgattcc atagaagcaa gattgaaata ccgagatgag tcatttttaa
    47761 ggataaataa tacggagttt ttaatgacat ctgataaccc ttgtgatatg tatttaatag
    47821 atcttgtaga agacgatagt tttatcgaaa ttgtggtcta tgatgatgga ccaagtggag
    47881 atcctgtgtc aactttttac agatatgatg ggaaaaatat ttatgaatta ggtcattttc
    47941 acactcacat agatacagag gatacaatct atagttatta tgggaatgtt cttactgatg
    48001 ggaagggaaa ttttttacca ccagactgta ttgagaaatt tattttgcct aatattataa
    48061 caggttatta ttctatagaa gcgcaacaac tggtttatca tcctatcgac tatgagaaat
    48121 atttagtgga tgaatatact atcgcagaag attttggagc atatttccat gaagaagatg
    48181 tttatgaaaa ttttaatgat gaagaattat atcttacatg ggattctgag caaataagac
    48241 agttcagcaa gggagaaaaa actaaaataa ttgtttttgg tgattattgg tatggtattg
    48301 aaagggaaga cggaacaaga ggaattttat atttctggac aggagattaa gtattagctg
    48361 agccgggatg attcaaaatg gttggtgcag gagaaataga ttatttagtc taaataagga
    48421 gtttattata catgtacggt ttaaaaattg aaaatttagc tcttgaaaaa aaagcaggag
    48481 agttgcctgg ttatattcat catagaattt ctaatgaagt ctctgatttt agttttttct
    48541 ttaaagactt cgttgaaaga ctggatgatt attattggat tcttgaaaaa ggaagtactt
    48601 atatatcatg ggagagccca gaaaaagatg atcataattt tgagttcggt ttatttaaag
    48661 atttttatga agaagtgtac gaggagtatt ccgtttatag aatgtatgat aataaagttt
    48721 ttaagaaata tattcattat attatagatg attggaatac aattttttgt tttaagaagg
    48781 aaaagattaa attaaaagat ttcattatta gtattaatac taaaagagga caggataaag
    48841 agaacttttt aaaagaaaat atagatttta tttttttaaa tattgatggc ttattctggt
    48901 tatttttttc aaatgatgcg ttttatttaa atgatattag attaagtctt gcaaagtttg
    48961 aatcaaaagt caaacttaca gattgctgtt tatcagatta tatcaatatg attgctttta
    49021 cctgaatctt attattaaag acagtattga tagtatttga gcatatattt ttactaaaga
    49081 cgatttgact gctgaataag tatttgtgtt tgggtgttgt ttatgattag ttttattaaa
    49141 tcttataaaa ataaatcact tctttatttt aaatgaagtg agaaagaaac agaagattat
    49201 ccaggtataa gatttgatat caaaatatcc aatgaatact ttagaactat aatagctgat
    49261 gtttgtttcg aaattgaaga actgaagaca tttgtttcta attgtcaggc gttattaaat
    49321 aatcatctaa atgaatctgc acgggtgtca atgaatccat atcagattaa gtttgagata
    49381 atgctacttg atagagagga cattatttaa tagaatatta ttataccaat tacagatata
    49441 tagaaaggca tttgctggaa tataatcttt cagtggattt ggaacagaat atcagagtct
    49501 gtataacttt agaaattcta cagataaaat attaatgatg gtatgataag atattatcag
    49561 atttgcaata attaagcttg taagctgcaa tatcttatca cattgagata aaagtaataa
    49621 aaaattggat aggagtgaaa atgatgggtg ctgagttttg gtcttgtctg gcagctatgt
    49681 tttctttagt aattttttgc tacttaattc aattgcataa tgatattttg cgattgaaca
    49741 ggaaggttaa taaattggca gaacatatgg gactggctga taatatggaa ccattaaatg
    49801 atgaagcgtt taaagagttg ataatggctg gagaaaaaat taaggccatt aaaaggtaca
    49861 gacaattaac cggggaagga ctaaaagaag catcagatta tgtagatgcc ttattactga
    49921 aaaataagta acattattaa agttaacttc tatgtagtaa agtcacctct tatcaagcaa
    49981 ggataggagg tgattttgtt tgaatatttt ttattgacta caaatttaac ataaattatt
    50041 atgataataa tatgtaaaag tcaaagtata ataattaata ttgttctttt gtctttaata
    50101 atttttgtaa aatgaatttt gagcttatat atttgaagta gggaggcttc tttatgatta
    50161 gaataacaga gagcagagat gctgatttat tagctttatt aaattatgat gtgcaagaga
    50221 ttcatgcaga aattgaacct atgcttttta agcatcataa tcaggaaaat atgaaagctt
    50281 tgtttaaaga ggcattagaa gatgagaata tttatgcttt tgttgcatgg gataatgaca
    50341 gaccagtagg ctatgtaatg atatcaaaaa tcaatttcga agaaaattat ttcagaaaaa
    50401 gttattcagt tgtttatatt gagcatattt gcgtgttaaa ggattataaa ggacatggta
    50461 ttgggaagca attgattgag aaggttaaag aatttgcaag agataataat attagaagag
    50521 ttgaattaga ttattggaat aagaatgaaa aggcaggtca gttttttaga agccaaggtt
    50581 ttaaaacatt caatgaaagg atgtatctgg aagtatagaa ttttgtcagg gaggaaatta
    50641 tgaagtttaa cgtttatgag ttacccctgg aatcaaaact atataaatta tatgatgtaa
    50701 tagaagtacc agtccaattg agagtagttt atccccaggg aaaaacacca gcgattttcg
    50761 caaatgcaga gggttttgct tttttagaaa gcatttttaa aattgcttct gtaagcatga
    50821 aagaaaactg tttaatcagg ataaaggaca attctaaaac agttgaaagt tttcatgcct
    50881 ggtatcccgc ttcgacggaa tttcatttag atctggttat ttttaatcat caatatacac
    50941 aaataagcag taaaaaaata aagagattat tgatgatgct ggattattgt aaaaagaaaa
    51001 taatgaatat ccgggttcct gaagttcatt acaagaatga taactggtgg tcactggagg
    51061 gtgaacttca tgtaaataaa agaagtaatt ttttggtgat ttcatcaaat agtaatggtt
    51121 atttgtatat ggctgacgag gcggacagat tttgtaatat agaagataat gaagaagagt
    51181 attcagctca ttctcactta tttgcactgt gtaaagagga agatcttttg gatttccgct
    51241 attattatga aaaaagttga gagttcttca tagtgggttg tcaggaaaag gtagatagaa
    51301 gaagagaaat aatatggaga aagaaaaaac agtcttgaat aataataaaa acattttgca
    51361 gaccattttt catattatta tgggattctt gatatcagtc ttcagtcaaa ttttaaggat
    51421 agtcatcatt gacagaacat attttttaac tagactgaat gagggtttat ttactttttt
    51481 gacttttatc cattattttt acttcttttt atttattttc aggaatgagg tatgctggaa
    51541 taaaatctgc tgatgtaata tgattattta taacaggtat aacgactagc gttatgtttt
    51601 cattgattac ttatttatat aataacgttt ggaatgcaat tgttatacat attttgtgga
    51661 atttattttt ttctggattt ttttggatat cgccgctgga aaatgatgct gctttaatca
    51721 attatattat tgacagtaag aatgttttaa ttacgggtag cagatttgga atcgggtctg
    51781 gcctgccagc aataatggta tacactgtag ttattattat gcttttattc ctggggagaa
    51841 atcaaaaagc tgacaggaag gatgtaaacc atatcggatg aagaaggaga gaaggttttc
    51901 atgaagattt tagattttaa taaagcgttt cttgaagctt gtgttcattt atatattgaa
    51961 acttttaatg ctgccccctg gaatgaccat tgggattacg aaactgcttt aagaagattt
    52021 ataaaacacc aggtttttta ggaaaagtcg ttcttgataa tggagaaggt aaaggagcag
    52081 tcatgcgaaa tattgaagcc tggtatgaag gggattttta tgatgtgaag gagttctttt
    52141 tacataaagt gttaagaaga aaaggagtcg gtagtcggtt aatgtatcag ttatgtcaag
    52201 aattaaagga tagaggagtt aaaactgtat ggttaatcac tgctaaaata gtatggcaga
    52261 gaattttttt atataaaaaa cggttattca agagcagaaa aaatgattat gatgcttaaa
    52321 gagattgatt agggcttatt ggtatttttc taataagccc tttatgttta tgattttaat
    52381 ttttcggcgg ggcaattgac ggatatatcc ttcatcttct aaggctgtaa gttttctgct
    52441 gatactttca ggggtggttc ctaagtaaga ggccagatct ttctgactca tcggtaaagt
    52501 gaattcctta tcttgatttt gggcagattc aattaaaaaa tcaacgagtc ttttttcaac
    52561 ggaaagggta gcaaaactgg tgctttgttt ttcaatagca tccaggcgtt ccgaaagtat
    52621 agcaagcatt ttaattgata tggatgggta tttttctaaa aacttttgca agtcaggacg
    52681 agtaatcatg cagatatttg tattttccat ggcttctgca aaagattcat gaacggattg
    52741 acgaaacaaa gccaattctc ccgtaaagtc tcccggattt aagatatgca gcagttgttc
    52801 tttgccagac tctgataagc ggtagatttt aactttaccg cttttaacaa tataaagaga
    52861 atcggattcg tctcctggtc tatagatgat ttcgcctttt ttgtatgaaa gggatttaat
    52921 ggattttgaa atttcttcca tttgatgtct ttctaaatgg ttgaatatag gcaccagagt
    52981 tatgcaggat tgattattat gccttttatc agtatgacag caatgatgac cattgttcga
    53041 atgtttatct gctttttgac tcatgatttc actcctttgt aataaaaagc agcaaggtgc
    53101 tttagtaata atatatattt ttttatgcca gatagcaatt tataatttat attacggaag
    53161 caataaacga aaaaatctgc tttaagtata atacttaaag cagattgaaa gggggaatta
    53221 ttcaggcttt ttagcattta tctcctttga aaattgcttc gatgaagtct tcaggatcgg
    53281 tgagtacttc acagatgcag tcaatgtcaa caaatttgat tttaccatca tatcttgcaa
    53341 taagagaatt tctttctaca gcaagaactt ctacttcttc atattttccg gattttaata
    53401 cgataatcac ttcttcatca ataagtcctt ttaataattc tctgatatta gcgggaaggg
    53461 tagcagaatt gcgacctttg tcacagcctt ttcctcttct gtcgaattca gcttcggata
    53521 ctcttctcat tttttcacct cctttttgtc tcttttgata ctaatatatg aatctatgaa
    53581 gggacatgtt acaaaaattc gacaaaaaaa tttattttgt tacagaggat tacttattta
    53641 taatccttat ttttcttaaa atagataata tcataatttt tataatttct tactatttat
    53701 taaaggttat ttaattaaac taagataaga agataggctt atttttaact acttttcact
    53761 gtgctattat aatggttaga gaattactag aaggatgggg gataatatga ctttgcagca
    53821 attaa
//
LOCUS       DAOSUD010000005        45998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_547_length_45998_cov_23.702240, whole genome shotgun sequence.
ACCESSION   DAOSUD010000005 DAOSUD010000000
VERSION     DAOSUD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 45998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45998
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_45998_cov_23.702240"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PLG49_01480"
     CDS             complement(<1..1603)
                     /locus_tag="PLG49_01480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ16013.1"
                     /translation="MKKMSFNKMILLLSLILTLQSFLPNMLYAATLTETTPSGIPYDD
                     LGKEIEKYIEAHKDVCASVSVAVFKNNETLFSTQYGFMNIEKQIPVNEETVYEWGSIS
                     KLLIWTSVMQLYEQGKIDLNKDIREYLPDNFLTKLSYDEPITMLHLMNHSAGWQETTY
                     ALEVSSIDEIVDLETALRNTEPPQVNKPGTVTAYSNWGAALAALIVQEVSGENYVDYV
                     HKHILEPLGMNQTSIAADYSDNQWVQEQRKLLNCYWITDTYRENLGDGISYILLYPAG
                     AATGTLKDLMTFAKAFVPKEGETSPLFQKKETLERMKETSAFYGDTDIPRVCHGFLTL
                     QYTKDIMGHPGNTNGFTSTLMFEPESGLGVVIMSNQLGETVFNYGILSLIFGDYGNHQ
                     RVLDNPVLLKNDISGIYTSCRSYEKGFASIYKYIGGLLPLSKTEDENIYKLSLGKGKV
                     IRIADHQYIMDNENALRYLMVETKNSDGHTVLQMMGCVDMVKEDPLSFGVKMISILLF
                     FIIILISIFKILFKGLFFVIRKIGRK"
     gene            complement(1718..2659)
                     /locus_tag="PLG49_01485"
     CDS             complement(1718..2659)
                     /locus_tag="PLG49_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOQ16014.1"
                     /translation="MVNFLKNKAFKEISIIIFLGILYLLLVHDSLDNNMRKQESKLEN
                     EIKKEAGEKEPFKENVHFILNETGDTIEKRYNPPEGFERIPVGEDSFAFYLRNHKLKA
                     YGEKALYYHGKEKNPQGIYDSVFDVEIGDRDLHQCADAIMLLRAEYLYQQKRYDEISF
                     DFTNGFKADYQNWLEGHRIKINGNHVTWVKEASYDDSYSNFRKYMDLVFSYAGTLSLE
                     KQLMSVNMEDMQIGDVFIQGGSPGHAVIVVDMAENKASGEKVFMLAQSYMPAQQTQIL
                     INPLDKDISPWYRLDSLEESLITPEWVFKRTDLKRFP"
     gene            complement(2853..3317)
                     /locus_tag="PLG49_01490"
     CDS             complement(2853..3317)
                     /locus_tag="PLG49_01490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16015.1"
                     /translation="MIHYKKLNLSDLKEARELVWNVFLEFVAPDYSTEGVENFKKFID
                     ENNLKDNVNTGNMEFYGAFTEGRIVGVIAVRGCNHICLFFVDKEFHGQGIARNLFELV
                     KKNLSVGDKVEKVITVNASPYAVKIYEKLAFNVVDNEQEKDGIRFTPMKYVF"
     gene            complement(3356..3853)
                     /locus_tag="PLG49_01495"
     CDS             complement(3356..3853)
                     /locus_tag="PLG49_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16016.1"
                     /translation="MDDIVIIKAKIDYVESFCKAVDTVAREKKYLASTEGFPLETTRA
                     FVEIIEKNDLAQFYALKDGIVIGWCDILPKSFEEMKHVGSLGMGVLAEYRGKGIGSRL
                     IETTIQHAKTRNGIEKIELEVFESNTNAIKLYEKYGFVYEGKRFKARKLDGKYDNIIL
                     MAKFL"
     gene            complement(3907..4725)
                     /locus_tag="PLG49_01500"
     CDS             complement(3907..4725)
                     /locus_tag="PLG49_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16017.1"
                     /translation="METILKFVEVKDYLTKSNLPDCDYVINPYIGCTHACKYCYASFM
                     KRFTGHKEDWGSFIDIKLCHKPVNLKKIENKSVFLASVTDCYNPFEEEYQITRKILEQ
                     LVHANCHVGISTKSKLILRDLDLLRKIKDLTVSISINTLDEKFREAMDQASSIQDRIA
                     TLKELHKQGIHTVLFMSPIFPFITDFKAIIEETKNFVDEYWFENLNLRGSYKKTILDY
                     IQLAYPQFINDYKRIYFRGDKQYWIELSEEIEAYCKQNNINYINYFYHEELVKK"
     gene            complement(4917..5504)
                     /locus_tag="PLG49_01505"
     CDS             complement(4917..5504)
                     /locus_tag="PLG49_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOQ16018.1"
                     /translation="MKKDLSEMSKEELWRLFPIILKEHNPQYKEWYESEKNSLLQLIK
                     KEDIYRINHIGSTAVSGLMAKPTVDILLELNQCCDYFQLIKRLKENHWILMSEEYEPG
                     LWLVFNKGYTAEGFEEKVFHLHVRYLGDWNELYFRDYLIDHPEVSKAYGLLKMSLWKK
                     YEFNRDAYTQAKSEFINSYTKKARLEYPRRYDVYS"
     gene            complement(5646..6083)
                     /locus_tag="PLG49_01510"
     CDS             complement(5646..6083)
                     /locus_tag="PLG49_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16019.1"
                     /translation="MDETKKEQMISVLDKVEEDLMQEDIYLKFGAIGHQKYICATKEG
                     LIKLGIFLIKSAFSQNRMISLCSEDSWLDWVDPESEEAIDYIEIVDNNCIEEIKKSNE
                     KRKLSVFESTILIFIVILVLIAIGLMPLLIIQLLEFLIEKFMV"
     gene            complement(6190..7134)
                     /locus_tag="PLG49_01515"
     CDS             complement(6190..7134)
                     /locus_tag="PLG49_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ16020.1"
                     /translation="MDIKKFFTKKHYVSLIAIFCSLLWGSAFPVLKVSYEEIGLKADD
                     LYAKVVFAGMRFCLASVLLFIIIHWIMKISLRINKKQFLSFFGLGVMQTTLQYFFFYN
                     GLANVSGIKGSVLGSISAFFVVLFAHFIYSNDKINLRKIIGLMAGFGGVIIASLGKEG
                     QNTFSLSFSVIGEGFMILSGITSALGSIWSKELAKKTHPFVITAWQMFLGSLVLLLFG
                     IGRVSVETFTFTPKAWILFIYSAFLSATAFSLFNTLLKYNKAGEVSIYKIMVPVSGSL
                     LSAMFIPGENFTIKLFLGLVLVSIGIGVVNSYKSENPS"
     gene            complement(7512..8051)
                     /locus_tag="PLG49_01520"
     CDS             complement(7512..8051)
                     /locus_tag="PLG49_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HOQ16021.1"
                     /translation="MERSFFVRFAVLSAVIIGGAFLFHMYNTNHFESKEEAYKYIMQD
                     IETYFINIENYFEVAGRDEELIVDKLMDDGSYQAKNPFNLRTVTFEKYDKLNCAKKFT
                     NKQPKYEELDTSMRNLIKEFNQALKITEDLLQYYVHKDYEKDYFDKARKLHKKLLIQV
                     EVLREAKEKTDALYEQASF"
     gene            8420..9091
                     /locus_tag="PLG49_01525"
     CDS             8420..9091
                     /locus_tag="PLG49_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ16022.1"
                     /translation="MKKLLYITVNSKPEELSASKTVGRAFVNKFIKKYPEFYVEELDL
                     YKEHIPRLEYQYFEDRNCVVNEEAMKKLPEKEQKEIQRINQLCNQFIEADLYVIAAPM
                     WSLSFPAPLKEYIDCIVQNHKTIHFPEKNDKPEGLLNDKERAVIYIQSSGGNIPWLMG
                     PIFNKGIDYVEDIMKLMGINQFEKILVDGTGTTQEERKTAIEKAMEKIDKVIENIKIK
                     EAVLL"
     gene            complement(9139..10113)
                     /locus_tag="PLG49_01530"
     CDS             complement(9139..10113)
                     /locus_tag="PLG49_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ16023.1"
                     /translation="METVVKTKGKRLLKRLIKILVVIMLILVIAFLGFSYYLGKEIAV
                     QMLMQNEGNDTNANSYKQLEEWGYDYKYFESLYKGEKINLIAGDGNEVPVTFFSTDGN
                     YDRDTVILVHGAGGDHVSMYPIAEFYLQNNWNVAAMDQRASGTSKNPYVTFGHFEKND
                     IVPVVEFIKKNTNNKKIVVHGQSMGAATAGLYAATEHANQNIDYCILDSSYDSMEGIF
                     SVIWQSMNTGLPYEYAVTCGDWYLKHRYGFSFEDVDVVAAQTNNKIPTLVIHGTKDDF
                     CFASMGKAIYKNISSDKKELWLVESRHIEACIDYPEEYEQKVLSFLAD"
     gene            complement(10162..10593)
                     /locus_tag="PLG49_01535"
     CDS             complement(10162..10593)
                     /locus_tag="PLG49_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16024.1"
                     /translation="MAGWETEIIRLFKRISEMIKPFYGDIFILNDYIHSRTVLCQDDM
                     TQKHPIMGYWWNGIPRKSGLAVVFGEPLLKYIKISRDYESLDNGCKLFQKTRTIKKMN
                     YTEVLKYQLGFISLMLSIIKFLIWEVFLVFIQEFGLLKDQN"
     gene            complement(10539..11288)
                     /locus_tag="PLG49_01540"
     CDS             complement(10539..11288)
                     /locus_tag="PLG49_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16025.1"
                     /translation="MSYDLKIYTTQKQDYNLLVNQFNMIENEECLILIHKDYQIVISR
                     EAVLNDEDIPQHIYNQLPGIRFLIECSLEPGIAEVKKIEELYKVAKVLAKNGIGLIED
                     LQTNDIVLPRGIKRLPDIDKTERFSIVKLSWWFNHYLNKEDINGLLKIIERQIPEILP
                     KRYGFCEPPKEKFTSLEAFEAFFAENIHNNMIWYPHKPVESVYITVPDPIGPGPIGYR
                     FGYLPFQLMQQYYPWQVGKQKSSDCLNVLVK"
     gene            11619..12323
                     /locus_tag="PLG49_01545"
     CDS             11619..12323
                     /locus_tag="PLG49_01545"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOQ16026.1"
                     /translation="MNKLENHHSNRIWEIDFLRGIALILMMLFHFLYDLNAFYHVEIP
                     YWDSFWVIEGKISAVLFMILAGISSTFSKNNLKRGLKILLVAMGISLVTYIIFPSEYI
                     RFGILHMLGSSMILYHFIYNISLSFTLMISLIIFLLGYLFNHFYIDTNLLIPFGIMSR
                     DFVSMDYYPLFPWLGVFLIGTIIGKSIYKEKKSILQKFYRNNFLSFLGRHSLIVYLIH
                     QPVILLLLYFFFSVRN"
     gene            complement(12403..12591)
                     /locus_tag="PLG49_01550"
     CDS             complement(12403..12591)
                     /locus_tag="PLG49_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16027.1"
                     /translation="MFESVLMVFIACASITTMVITKLYFSHKEKMEEIRANARQDHLN
                     KKQEDYFVDLRDEELHPN"
     gene            complement(12737..14407)
                     /gene="ilvB"
                     /locus_tag="PLG49_01555"
     CDS             complement(12737..14407)
                     /gene="ilvB"
                     /locus_tag="PLG49_01555"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOQ16028.1"
                     /translation="MQLTGAQILIECLKEQGVDTIFGYPGGAVLNIYDELYKHQNEIR
                     HILTSHEQGAAHAADGYARATGKVGVCLATSGPGATNLVTGIATAYMDSVPLVAITGN
                     VAVPLLGKDSFQEVDITGITMPITKHNYIVKDVNELADTIRKAFYIAKEGRPGPVLID
                     IPKDVTALKAEYKYREPKPIPAYSERIREEDIERALKLINRAKRPFVYAGGGVGYAGA
                     DQELLTFVEKINSPIACSLMGQGAFPATHRLYTGMIGMHGTKASNIAATECDLLIAIG
                     ARFSDRVVSNLQRFAPRAKILQIDIDPAEINKNVRTFCYILGDIKTALTMLNDRLKPR
                     EANEWNEKIEKLMQEYPVTYTKNGNLKPQAIIEKIYEKTQGDAIITTEVGQHQMWVPQ
                     YYKFTKPRTFLSSGGLGTMGYGTGAAIGAKIGMPDKRVVHIAGDGSFRMNCNELATIN
                     AYKLPIIIVLINNGTLGMVRQWQTMFYEGRYSATTLDRGPDFVKLAEAYDIKGYRVTS
                     EEELEKALNEAFALNEPVLLDCHVGIDEKVLPMVPPGKAIEDVITDDC"
     gene            complement(14450..16111)
                     /gene="ilvD"
                     /locus_tag="PLG49_01560"
     CDS             complement(14450..16111)
                     /gene="ilvD"
                     /locus_tag="PLG49_01560"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOQ16029.1"
                     /translation="MRSDLAKSGVEKAPHRSLFKAMGYTNEEIKQPLVGIVNSKSEII
                     PGHMHLNTIAEAVKAGVRMAGGTPVEVPAIGVCDGIAMGHIGMHYSLASRELIADSIE
                     TLAMAHSFDALVLIPNCDKIVPAMLMAAARINIPAIVVSGGAMLPGKIEEKRLSLTDV
                     FEAVGAVKSGKMTEEELYQYEETACPGCGSCSGMFTANSMNCLTEVLGMGLPGNGTVP
                     AVYAERIRLAKKAGMMVMKLLKNSIRPRDIMTKEAFYNALTVDMALGCSTNSMLHLPA
                     IAHEAGVELNLEIANEISERTPNLCKLAPAGPHFIVDLYEAGGVQAVMKELSKKNLLN
                     LSLITVTGKTLGENIASAVNRNPNVIRPIDNPYSKTGGIAVLKGNLAPDGCVVKRSAV
                     AEEMLQHRGPARVFNSEEEVIEAIFSGRINKGDVVVIRYEGPKGGPGMREMLAPTSAL
                     AGMGLDKDVALITDGRFSGATRGASIGHISPEAAEGGPIALVEEGDIISIDMINGTIE
                     LEVSDEELKRRKEKWQAPEPKVKSGYLARYAKLVTSASTGAILKN"
     gene            complement(16123..17007)
                     /gene="ilvE"
                     /locus_tag="PLG49_01565"
     CDS             complement(16123..17007)
                     /gene="ilvE"
                     /locus_tag="PLG49_01565"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOQ16030.1"
                     /translation="MSDELIIYLDGEYVKESEAKVSVFDHGLLYGDGVFEGIRAYNGR
                     IFKCKEHIDRLYAAAKAIMLEIPLTKEEMKEVLLETCRRNHLRDAYIRLVVTRGKGDL
                     GLSPTKCPKPSVFCIASSITLYPPEMYEKGMPIITASQRRNKATIVDPQIKSLNYLNN
                     ILAKIEANRAGVPEALMLNHDGIVAECTGDNIFIVKDGVLYTPPIHIGILDGITRRTI
                     IDLAKDMGIEVYEKEFTLFNVYNADECFLTGTAAEAIAVTNVDGRVIGNGVAGPITKK
                     LLEAFQKYANSTGEPIYE"
     gene            complement(17033..18103)
                     /gene="leuB"
                     /locus_tag="PLG49_01570"
     CDS             complement(17033..18103)
                     /gene="leuB"
                     /locus_tag="PLG49_01570"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOQ16031.1"
                     /translation="MNFNIATIPGDGIGPDIVEQTILVLDKIGEKYGHRFNFTEVLAG
                     GVAIDKTGEPLPQETIDICKKSDAVLLGAVGGPKWDNLPGDKRPEKALLGLRGELGLF
                     ANLRPALLHKPLKDACPLKAEIIGDSLDILVVRELTGGIYFGERGRRTGKYGEEAYDT
                     ETYSVKEVERIARVGFEAAMKRNKKVTSVDKANILESSRLWRAVVEEVAKDYPQVELN
                     HMYVDNAAMQLIRNPKQFDVIVTSNMFGDILSDEASMLTGSIGMLPSASLAEGSFGLY
                     EPIHGSAPDIAGQNKANPIATILSAAMMLRYSFGLEEEAQAIENAVTKVLEQGYRTGD
                     LMSEGMKLVGTKEMGQLIINEL"
     gene            complement(18297..18785)
                     /gene="leuD"
                     /locus_tag="PLG49_01575"
     CDS             complement(18297..18785)
                     /gene="leuD"
                     /locus_tag="PLG49_01575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOQ16032.1"
                     /translation="MKVQGRVFKYGDNVDTDVIIPARYLNTSDPQELAKHCMEDIDPE
                     FAGKVRKGDIIVADKNFGCGSSREHAPIAIKASGVSCVIASTFARIFYRNAINIGLPI
                     LECEEAVKGIDAGDEVEIDFSTGTITNKTKNETYKAQPFPEFMQKIIASDGLIKYIQQ
                     KF"
     gene            complement(18798..20060)
                     /gene="leuC"
                     /locus_tag="PLG49_01580"
     CDS             complement(18798..20060)
                     /gene="leuC"
                     /locus_tag="PLG49_01580"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOQ16033.1"
                     /translation="MGMTMTQKILAAHAGLDSVKAGQLIEANLDLVLGNDITTPVAVK
                     EFRKIGVDKVFDKSKVAIVPDHFTPNKDIKAAEQCKFIREFAYEKGIENYFEVGEMGI
                     EHALIPEKGLVVAGDVVIGADSHTCTYGALGAFSTGIGSTDMAAGMATGKAWFKVPAA
                     LKFVLKGKMSKWVSGKDVILHIIGMIGVDGALYKSMEFAGDGIASLTMDDRFTIANMA
                     IEAGAKNGIFPVDEKTIEYMKEHSTREYKIYEADEDAEYEKVYEIDLSKIRPTVAFPH
                     LPENTRTIDEVGDIKIDQVVIGSCTNGRMEDLRVAAKILRGRKVAKGVRCIVFPGTQN
                     IYLQALKEGLVEDIVKAGAVFSTPTCGPCLGGHMGILAKGERAVSTTNRNFVGRMGHP
                     ESEVYLASPAVAAASAITGKITNPEDLE"
     gene            complement(20076..21668)
                     /gene="cimA"
                     /locus_tag="PLG49_01585"
     CDS             complement(20076..21668)
                     /gene="cimA"
                     /locus_tag="PLG49_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOQ16034.1"
                     /translation="MKKQIAIFDSTLRDGAQGEGISFSVADKLKIVETLDELGVAYIE
                     AGNPGSNPKDLEFFERVKNMSLKNARLAAFGSTRRRDTTVEEDNNIQSLLKADTPVVV
                     IFGKSWDFHVTDIIKTTLEENLAMIKETLRFFKEKGKEVIFDAEHFFDGYKANPEYAM
                     ATLSAAVEGGADCIVLCETNGGAFPDEVYEITKKVVENFNLHVGIHPHNDGGMAVANA
                     IMAVKAGASHVQGTFIGIGERCGNVNLSTVIANLQLKKNYQCIPDDKMVALTQTARRI
                     AEIANVSLTDGMPYVGKSAFAHKGGMHIDGVSKASHSFEHIDPELVGNERRFLMSEVA
                     GRSTILAKIQKFNAGIDKDSPETKNIIDKLKKLEHIGYQFEGAESTFELIIRKELGKY
                     KPFFELEHFKIIGEHPAGEESFSSSALIKVKVDGQTEITAAEGDGPVNALDKALRKAL
                     EVFYPELRDVHLTDYKVRVLDTKQATAAKVRVLIESTDGEDIWTTVGVSSDIIEASWI
                     ALVDSIEYKLIKDIEKKFKAYL"
     gene            complement(21698..22693)
                     /gene="ilvC"
                     /locus_tag="PLG49_01590"
     CDS             complement(21698..22693)
                     /gene="ilvC"
                     /locus_tag="PLG49_01590"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOQ16035.1"
                     /translation="MAKLYYEHDCNLSLLKGKKVAIIGYGSQGHAHALNLHESGVDVI
                     VGLYKGSKSWPKAEAAGLKVATAAEAAKEADIIMILINDEKQPKLYKESIEPNLSPGK
                     SLAFAHGFNIHFGQIVPPEDINVFMVAPKGPGHTVRSQFLEGKGVPCLVAVYQDATGN
                     AKDLALAYAAGIGGARAGILETTFKEETETDLFGEQAVLCGGVSELMKAGFEVLVEAG
                     YQPESAYFECMHEMKLIVDLVNQGGLSYMRYSISDTAEYGDYSVGKRIITEETKNEMR
                     KVLKEIQDGTFAKNWILENQANRPSFNARRKMEQEHPIEVVGRELRKMMSWIQSK"
     gene            complement(22730..23230)
                     /gene="ilvN"
                     /locus_tag="PLG49_01595"
     CDS             complement(22730..23230)
                     /gene="ilvN"
                     /locus_tag="PLG49_01595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOQ16036.1"
                     /translation="MKRYVLSVLVENHSGVLSRVSGLFSRRGYNIDSLTVGVTEDPNT
                     SRMTIVVKGDKYIVEQIKKQLNKLIDVIKVIELKPESSVYRELILIKVSATTKQRAEI
                     IEIVNVFRGRIVDVVNDSLIIELTGDESKIEALINMLEPYGIKEIIRTGLTALERGNK
                     VIKEHN"
     gene            complement(23266..24888)
                     /gene="ilvB"
                     /locus_tag="PLG49_01600"
     CDS             complement(23266..24888)
                     /gene="ilvB"
                     /locus_tag="PLG49_01600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOQ16037.1"
                     /translation="MKATDAIVECFRKENVSCVFGYPGAAIMPLYDALRRSEIEHVLV
                     RQEQSAAHCASGYARASGKVGVCTVTSGPGATNLITGIATAYMDSIPIVAITGQVKST
                     LIGRDVFQEVDITGATEPFVKHSYLIKNAKDIPRIIKEAFYIAKTGRPGPVLIDIPSD
                     ILVEDIDFYYDEEVNIRGYKPTYVGHAGQVKKAFTRLKESKKPIICIGGGVISSHAEK
                     ELLDFVEKTKIPVVSTLMGLGGIDHNSSYYIGMIGSHGHHYANRAVAEADVIMFIGAR
                     LGDRATGENKLFKNEPDIIHIDIDPAEIGKNLGTSIPIVGDAKNVLKQLLEMAQPLQT
                     EDWLRELWELKEKHKKFKEDIKTNKEYVNPKYAIELLSDVLNHDAILVGDVGQNQIWA
                     ALNFKISGDRKFFISGGLGTMGYSLPAAIGSAIANKNRQIVAVVGDGGFQMSLYELAT
                     IKQNHLKLIILLFNNMGLGMVREMQYKFYEAEYAVDLSSNPDFIQLARAYGIEGIRVS
                     SNEDLKEAFTKAMNTESAFLIECIVDPKESTL"
     misc_binding    complement(25005..25258)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(25305..26930)
                     /locus_tag="PLG49_01605"
     CDS             complement(25305..26930)
                     /locus_tag="PLG49_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ16038.1"
                     /translation="MDNKWNNKEKKKFKEFSKRGLFLLDNTYYKITTLGILLLLISMF
                     FTVIMRFNWNNNLFYEDKSSSSLIFFVLLALELIIFTIIIAFIMSQQKSQKKIFQLAY
                     FDSLTDLPNRKYFEDILPDFVEEVKKYNYKAALIYIDLDNFKVVNDTIGHALGDRLLK
                     QIGHLIRKCLRHSDFVCRFEGDEFVILLPGINNKDDVISVVERIIESLQIPFTSYEKK
                     FYITASIGIAIYPDDSDDIYTIVKYATMAMNRAKENGKNGYCLFESHMNTKLLEKYKL
                     EEDLRQALINQEFVLYYQPQIDLMTGEMVGVEALIRWMHPEKGCLSPLEFIPLAEETG
                     LIVPIGEWVIRAACNQSVKWQKRGAKDLRISVNLSARQFQQPDLVDMITEIIEETGMD
                     PKLLDLEITESVAMRDLELTKKVLGELREKKINISLDDFGTGYSSLNYLKQLPINTVK
                     IDRTFVDNITNDINQQTIAKAVIDLSHNMELYVTAEGVETWEQFNFLKYQKCDKVQGY
                     LFSMPLPLEEIEKIIFQDRNFIDKSDLVLSKSY"
     gene            complement(27089..27748)
                     /locus_tag="PLG49_01610"
     CDS             complement(27089..27748)
                     /locus_tag="PLG49_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOQ16039.1"
                     /translation="MEKNIELLKRILVFSKLSEEQLKDIAKLEIRKEYKKGSVIFFEG
                     EKGDAFYYILSGKVKMYKTDDEGKEIILNIFGEGSVIGEVTMFNDIDYPATAEVIEDA
                     VLGMIYNREIENLVASHKDLALQIIKVLTKRLYHSQMNVKEMALTNVYLRTKNVLLNL
                     AKEHGIKTSRGIELNLDITRQDIANIVGTTRETASRVMSELKKAGEIDIIGRKIIIKN
                     I"
     gene            complement(27891..29381)
                     /locus_tag="PLG49_01615"
     CDS             complement(27891..29381)
                     /locus_tag="PLG49_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOQ16040.1"
                     /translation="MKLAFLLLFILVMIIVGVYSKTRIKSSDDFFLGGRSMGAWMSAF
                     AYGTSYFSAVIFIGYAGGLGWRFGVSTSWIGIGNAVFGCWLAWKLLARRTRDITQRLD
                     ARTMPEFFEKRYQSKNMKFIAALVVFIFLVPYTASVYKGLGYIFESAFGISFNTVVLI
                     MALLTAVYLILGGYVATAINDFIQGIIMIIGSILMVFFVLKSPEVGGFSAALSKLSDL
                     EPGSGSFIPNASQFIPLMSMVLMTSFGVWGLPQMVHKFYAIKDEAAIKRGTIISTLFA
                     FIIGVSAYFTGALGRLFFIENGVAVMPQNNADMIVPLMLEKALPDALLGIIIVLILSA
                     SMSTLAALVLVSSSTISIDFIKGFVKNDLSDKKTTYYMRFFCIIFVLLSYIMAIFQSN
                     TIVYLMSLSWGVVSGMFLGPYVWGIWSRKVTSFGAWAGFIGGGIVIGGYIILQTFGIL
                     TVDMPIVSTIAMLVSFITVPLASVLGKEMSHEFIEKVFGKVEKTTV"
     misc_binding    complement(29431..29689)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(29798..31270)
                     /locus_tag="PLG49_01620"
     CDS             complement(29798..31270)
                     /locus_tag="PLG49_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOQ16041.1"
                     /translation="MFSFVYKEGEVNVYKNLCNGQWVESEKKQRIKIYSPVDDSLIGE
                     IQAQTKNDVDYAIECAKEAQKSWAFMPIRDRADIIHKAARIMEKYIEEIANILVKEIA
                     KSKSSAISEVKRTIDFMHFTAETAKRMTGETIYGDAFPGFDKTKISIVDRVPLGVILC
                     ISPFNYPVNLSVSKVAPALVGGNSVIFKPPTQGSISAMYLGHIFDEAGIPPGVLNIIT
                     GKGSEIGDYLITHPGIDMINFTGSTEVGQHIAKEAGMIPLLLELGGKDAAIVLEDCDL
                     DETAQNIVKGAFNYSGQRCTAVKRVLVMEEIADELVAKMVAEVKKLKVGKPEEDADIT
                     PLISDKAADFVQELIDDAINKGAKLLIGNKREGRYIWPTVFDYVTEDMRLAWEEPFGP
                     VLPVMRVKSIEEAIDISNKSEYGLQGCVFTKNIDKAFAIAQKLEVGTVQINNKTERGP
                     DHFPFLGVKASGLGTQGIRYSIEAMTRSKAIVINLDNDYK"
     gene            complement(31291..31620)
                     /locus_tag="PLG49_01625"
     CDS             complement(31291..31620)
                     /locus_tag="PLG49_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YddF family protein"
                     /protein_id="HOQ16042.1"
                     /translation="MKNYPIALFNGTVATTDGLYRVQGISVDDAKKLIEENGFISAIG
                     HKATAEIMSDLLGINIPMNRIDFKQEIGQKAVVFKLKKRPPEGSVLTKEQIEATGYEF
                     KLMERIE"
     gene            31777..32580
                     /gene="zupT"
                     /locus_tag="PLG49_01630"
     CDS             31777..32580
                     /gene="zupT"
                     /locus_tag="PLG49_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOQ16043.1"
                     /translation="MENVMFAFGITLFAGLATGIGSILAFFAKKTNTKFLSVALGFSA
                     GVMIYVSMIDIFTKAKDSLVLVLGETKGSWITVISFFGGMFLIGLIDTFIPSTENPHA
                     VHTVEDMNQPLSPKKDDLFRMGIFTALAITIHNFPEGLATFTAAIKDPSLGIAIAVAI
                     AIHNIPEGIAVSVPIYFATGSKKKAFIYSFLSGLSEPLGASVGFILLSGLFNDLTFGI
                     LFAAVAGIMIYISLDELLPAAEKYGEHHLSIYGVIAGMMVMALSLLLFI"
     gene            complement(32638..33036)
                     /locus_tag="PLG49_01635"
     CDS             complement(32638..33036)
                     /locus_tag="PLG49_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16044.1"
                     /translation="MINENRLYKDITGMEKILSIHPQFSWKKIKYIEAFNYYIDLNEF
                     GERSSYLKILFGLELSNHGLMKACFKFNGINNLIMRSIGGKYNQIMGFEIIDRLFLGW
                     DLEQRYLVRDYENGTIEFSCKAIELLQVDI"
     gene            complement(33242..34876)
                     /gene="hcp"
                     /locus_tag="PLG49_01640"
                     /gene_synonym="priS"
     CDS             complement(33242..34876)
                     /gene="hcp"
                     /locus_tag="PLG49_01640"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOQ16045.1"
                     /translation="MFCYQCQETAKGQGCTLRGVCGKSADVANLQDLLIYVLKGISMY
                     TTEGRKVGYESKEVNEFIMENMFSTITNANFDRNDFIHRIERALEVRENVKKAVAEAG
                     GKVEKITHDAAIFTVSKEEFDKKAETVGILTTENEDIRSLRELITYGLKGMSAYGKHA
                     INLGYDKNEIHAFLQKALAATLDDHLTIDDLVALTLETGKYGVDVMALLDEANTSSYG
                     HPEITKVNIGVRNNPAILISGHDLKDLEQLLEQTKDTGVDVYTHGEMLPAHYYPAFKK
                     YEHFVGNYGNAWWKQNEEFEKFNGPILMTTNCLVPPKESYKDRVYVTGSVGFEGIQYI
                     SAGKDGKKDFSAIIEHAKRCPAPVEIEKGEIIGGFAHNTVFSLADKIVDAVKSGVIKR
                     FFVMAGCDGRMKSRDYYTEFAKSLPEDTVILTAGCAKYRYNKLDLGDIGGIPRVLDAG
                     QCNDSYSLAVIALKLKEIFELNDVNELPISYNIAWYEQKAVIVLLALLYLGVKNIHLG
                     PTLPAFLSPNVAKVLVEQFGIGGITNVEDDLKMFLG"
     gene            complement(34995..36230)
                     /locus_tag="PLG49_01645"
     CDS             complement(34995..36230)
                     /locus_tag="PLG49_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ16046.1"
                     /translation="MKLQGKVVVAQGGGPTAVINQSLVGVVLESRKFQQVTKVYGAVN
                     GVAGIVNEDFVDLTQETTNNLEQVARTPSSALLSTRDKPDEKYCKEIFDVLKAHDVRY
                     FFYIGGNDSADTVRIVNENAKKSDYEFRAIHIPKTIDNDLVLNDHTPGYGSAARFVAQ
                     AFIGVNLDNRALPGVYIGVVMGRHAGFLTAASSLAQKYPDDGPHLIYLPERPFSIDKF
                     LSDVRKVYYQYGRCVVAVAEGIQDEKGVPVITKLMEDVEKDAHGNVQLSGTGALGDLL
                     AKCVKDKLKINRVRSDTLGYLQRSFIGCVSDVDQNEAREVGEKAAQFAIWYDVDGSIT
                     IQRTGFYSVNYELRRLEEIAGKTRVMPDHFINKEGNNVTDAFKYYLSPLVGSNFPVAH
                     RIRAPKAPKILDYRKTVGE"
     gene            37017..37253
                     /locus_tag="PLG49_01650"
     CDS             37017..37253
                     /locus_tag="PLG49_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16047.1"
                     /translation="MNSFKKCICLLISTLLLISSLSGCGILNNSKSTGSSQSGNTVSE
                     DTDNKKQEKQVGALIILCPWVSLFPGKMNIPVIF"
     gene            37199..37711
                     /locus_tag="PLG49_01655"
     CDS             37199..37711
                     /locus_tag="PLG49_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16048.1"
                     /translation="MPLGESFSWEDEYSSDILDPIAYTGTYTACIKSVKKLEDSEYEQ
                     YDLEKDENADAYLISIEMSGDITPTVGKVSYNSTIPNISGTRVPEGGGYMGAVTSGFD
                     GSISRVISDKYGLSTLLNNNENNQVDYSGEFIVYGYKEYENLLMIKVQKKYLDENVPS
                     DEKYIYFKLK"
     gene            complement(37716..38603)
                     /locus_tag="PLG49_01660"
     CDS             complement(37716..38603)
                     /locus_tag="PLG49_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOQ16049.1"
                     /translation="MMKELSLKKALKFVLLLFYIYVFYSIYTGNFVTEVSKKQNQWNQ
                     NNPFAVYREKQFLGAFETFAQASAHARRYQKSYIYYYNNKKMIWTNTIGLKESVILEA
                     PMISQLPELARGCEVTSLAMMLQSAGVQVDKMTLAKEIKKDPTPYQKKNGKIYYGHPN
                     AGFVGDIYSFENMGYGVYHKPIKALADQYLPGKVIDLTGCHWEDILYFLSQGSPVWVI
                     SNGSFKELSDDYFQEWHTPYGKIRITMKEHSVLITGYDKDYIYINNPLKNAPNQKVSR
                     KDFYASWKQMGRQAISYIK"
     gene            complement(38653..39306)
                     /locus_tag="PLG49_01665"
     CDS             complement(38653..39306)
                     /locus_tag="PLG49_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HOQ16050.1"
                     /translation="MRKGFFDLDGPFNRIGTFVFDMVFLNLLWFLFSIPLVTFGASTT
                     ALFYVVGKKVRKEDGYIFKDFWKSFKMNFKQATAIWLILILLISVLVFNMSNVALYGE
                     LGRYIFIVQAAVLLQLLIISLYIFLLLSRLYLTLSSAFKMAFVIGNKHFFTTILCIIL
                     FEILIIAIYIYPPVILFGVVIYAYLSYYLIGKILKRYLPKAEDGGQENEKQEQEMEE"
     gene            complement(39358..41514)
                     /locus_tag="PLG49_01670"
     CDS             complement(39358..41514)
                     /locus_tag="PLG49_01670"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOQ16051.1"
                     /translation="MGKILVIAEKPSVGRDIARVLGCKEKADGCITGERYIVTWAMGH
                     LVELLMPEDYDDKYKKWSLDDLPIIPEPMKIKPVKKTIKQFNIIKKLMHSKEVDSLIC
                     ATDSGREGELIFRYIYEAAGCKKPFKRLWISSMTDEAIKKGFESIRPGEEYDKLYYSA
                     KCRSEADWLVGMNASRAYTIKYNVLLSIGRVQTPTLSILVQRQKEIENFVSEDYSEVR
                     VDYGNFYGKWIDLDKNETKITDEAKVDAIVNLVKDKKGMVIEIQEEEKNQLPPLLYDL
                     TELQRDANKYYGFSASKTLSVAQVLYEKKKMITYPRTDSRYLSSDMKAVLVSVLNKLN
                     IPAYSQFVQPILEKESLPVTKRIIDDSKVTDHHAIIPTDTVPKLEKLTSDEKKIYDLI
                     VKRFLAVFYPRYEYTITKLITEIEKEHFISKGKRIRQLGWMIFYKEDSGKKKEEEMEL
                     PSVKKGDELFVKMAEKEKKQTSPPKPYTEAALLSAMENAGRFVEDEELKEQLKESGLG
                     TPATRAAIIERLIEVGYVERKGKVLYPTEKGMKLIEIVPEELKSPETTGKWEKGLSSI
                     AKNKMDPERFMDSIIEYVKQIVDESKKSDIKVSFPKESKKNSKRSSKKTKGLGKCPLC
                     NKGSIMENSKAFYCSQWKAGCKFALWKNSLEIYGLTITAEMVERLLKDKVIKGISYTL
                     PQTQERYMGDLSFMKNGKGMVEFINGKKLKNESSTV"
     gene            41707..42084
                     /locus_tag="PLG49_01675"
     CDS             41707..42084
                     /locus_tag="PLG49_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOQ16052.1"
                     /translation="MADESMNNRNRNDESFGIGHAIIRLIVTAIVLAITAFLTPGFSV
                     DGIWALIVASIVITLLDYLIEKITGIDASPFGRGVVGFIVSAAIIYVTQYFVAGFDVT
                     FWGAIIGALVIGVINAITPGRAM"
     gene            complement(42116..43411)
                     /gene="nagZ"
                     /locus_tag="PLG49_01680"
     CDS             complement(42116..43411)
                     /gene="nagZ"
                     /locus_tag="PLG49_01680"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOQ16053.1"
                     /translation="MLKKIGVLIMLILAFCLGCSAYYNNESSYDKDSTDKSKEEVKQE
                     DNNSEDNSSDKNNNEDNSSGLNPSEEIDPIKEKIKEMSLEEKIGQLVIVGFEGQSIDE
                     ETEKMIKEYYVGGVILFKRNVNDAGQLLTLINTLKRLNEDKIPLFISVDEEGGLVSRM
                     PAELINLPDSKSVGEKNDKDYSYKIGKIIAREIKAFGFNMNFAPVLDINSNPNNPVIG
                     PRAFGADVITVKEQGLAVMEGMQSEGIISVVKHFPGHGDTQVDSHLNLPLIEKDLEEM
                     KQFELVPFIEAINNGCDAVMIAHILFDKVDSQNPASLSQVIIGDILRNQFGFQGLVIT
                     DDLTMGAIADHYEMGEAAVKAINAGADIVLVCHGYNNEIKVLERIKKAVVEGEISGQR
                     LDESLYRILSMKMKYNINNEEIPSVDIDAINQEIRSLIN"
     gene            complement(43547..43684)
                     /locus_tag="PLG49_01685"
     CDS             complement(43547..43684)
                     /locus_tag="PLG49_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16054.1"
                     /translation="MRIKKEKVKDNCIRFVFKGFTNKNDKAGFLIILENAISSIGKKW
                     K"
     gene            complement(43761..45422)
                     /gene="leuA"
                     /locus_tag="PLG49_01690"
     CDS             complement(43761..45422)
                     /gene="leuA"
                     /locus_tag="PLG49_01690"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOQ16055.1"
                     /translation="MNYKKYKPYPTVDLKNRKWPNRTITKAPIWCSVDLRDGNQALKV
                     PMNLEQKLKFFDFLVKMGFKEIEVGFPAASETEFTFLRKLIEDNLIPDDVTIQVLTQA
                     REHIIRKTFESLEGVKRAIVHLYNSTSELQRRVVFKKSKEEIIDLAVEGAHIVKEFAE
                     KMPKGSIVYEYSPESFTGTELDYAIEICEAVLDVWKPSKENKAIINLPATVEMSTPNV
                     YADQVEYFIDHIKNRENIILSVHPHNDRGTAVAAAELAMLAGADRVEGTLFGNGERTG
                     NVDIITLGMNMFSQGVDPELDFSDADAIIKIYEESTKMEVSPRHPYVGELVYTAFSGS
                     HQDAIKKGMEAYKKDKPDHWEVPYLPIDPKDVGRSYDPIIRINSQSGKGGVSYVLEND
                     FGFRLPKKMQQDFSLIITDVSDRNQTELTPQMIHDIFIKEYMNIVEPYSLKNYKIDSQ
                     EDDIVVRVIIKKADQEEEIEGRGNGPLDAFCNGIKEHLSIDFKIVSYDEHSLDKGSSS
                     RAVSYIHIEDEEGNSFFGAGINTNISTSSIKALISAINKMISNRQ"
     misc_binding    complement(45481..45888)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        14207 a         9413 c         6376 g        16002 t
ORIGIN      
        1 cttttcttcc aatctttctg ataacaaaaa ataagccctt aaataaaatc ttaaaaatag
       61 aaatgaggat aataataaag aataataata tcgaaatcat ttttacccca aatgaaagag
      121 ggtcttcttt taccatatct acacagccca tcatctgcaa tacagtatgt ccatcgctat
      181 ttttcgtttc aaccattaaa taacgtaaag cattttcatt atccataata tattgatggt
      241 ctgcaatccg tatgactttt ccttttccaa gggagagttt ataaatattt tcatcctctg
      301 tttttgataa gggcaaaagt cctccaatat atttatatat tgaagcaaaa cctttttcgt
      361 aggacctgca tgatgtataa atcccactga tatcattttt taaaaggaca ggattatcaa
      421 gtactctttg atgattgcca tagtcaccaa agattaaaga caaaatacca taattaaaaa
      481 ccgtctcacc caattgattg ctcataataa caacacctaa accagattcc ggttcaaaca
      541 taagggtcga tgtgaaacca ttggtatttc caggatggcc cataatatct ttagtatatt
      601 gtaaagtcaa aaaaccatga caaactctcg gaatatctgt atctccataa aaagcgcttg
      661 tctctttcat cctttcaagg gtttctttct tttgaaacaa aggacttgtt tccccttctt
      721 tgggtacaaa ggcttttgca aaagtcataa gatcctttag agtccctgtt gcagccccag
      781 caggatataa taagatataa ctgatcccgt ctcctaaatt ttcacgataa gtatctgtaa
      841 tccaatagca atttaataat ttcctttgtt cctgtaccca ttgattatca gaataatctg
      901 ctgcaattga agtctgattc attcctaaag gctctaatat atgtttatgt acataatcta
      961 cataattttc accacttact tcctggacaa tcaaggcagc taaagcagct ccccagttag
     1021 agtatgccgt cactgttccc ggcttattca cttgcggtgg ctccgtattt cttaaggcag
     1081 tctctaaatc aacgatttca tcaatactgc ttacttccag ggcataggtg gtttcctgcc
     1141 agccggcact atgattcatt aaatgaagca tcgtaatggg ttcatcataa gaaagtttcg
     1201 tcaggaaatt atcaggtaaa tactctctta tatccttgtt taagtcaatt ttcccttgtt
     1261 cgtataattg catgacactt gtccatatca gcaattttga aatagaaccc cattcataaa
     1321 cggtctcttc gttgactggt atttgttttt ctatattcat aaagccatat tgtgtagaaa
     1381 aaagcgtctc attgttttta aaaacagcaa cagaaaccga tgcacaaaca tctttatgcg
     1441 cttctatata cttttcgatt tcttttccta aatcatcata aggaatacct gaaggtgttg
     1501 tttcagtaag agtcgccgca tatagcatat tgggaagaaa agactgtaaa gtaagtatta
     1561 gactcaataa taaaatcatt ttattaaaac tcattttttt cataaaaact ctcctttgtt
     1621 cacagcaaaa agattttacc tttcttttta taattactta cgcaataata aattctattg
     1681 tctgaaaaag ctgaacacag acaatagaat atccatttta aggaaatctt tttaaatctg
     1741 ttcgtttaaa gacccattca ggcgttatta agctttcttc caaactgtcc agcctgtacc
     1801 atgggctgat atccttatcc agaggattga ttaaaatctg agtctgctga gctggcatat
     1861 aactttgagc caacataaag actttttctc cactggcttt attttctgcc atatctacaa
     1921 caataacggc atgacctgga ctgcctccct ggataaagac gtcacctatt tgcatatcct
     1981 ccatattaac agacatgagt tgcttttcca atgacagggt ccctgcatag gaaaaaacca
     2041 aatccatgta ttttctaaaa tttgaataac tatcatcata agaagcttct tttacccaag
     2101 tcacatgatt tccgttgatt tttattctat gaccttccag ccagttttga taatctgcct
     2161 taaagccatt ggtaaaatca aaagaaattt catcatatct tttttgttga taaagatact
     2221 ctgccctcaa aagcataatc gcatccgcac attgatgcaa atccctgtct cctatctcga
     2281 catcaaatac actgtcatat atcccctgtg gatttttttc ttttccatga taatataagg
     2341 ccttttcgcc ataagctttt aatttatgat ttctcaaata aaaggcaaaa ctatcttccc
     2401 cgacaggaat tcgctcaaaa ccttccggtg gattatatct cttttcaatt gtatcccctg
     2461 tctcatttaa aatgaaatgg acgttttctt taaatggttc cttttctcct gcttcttttt
     2521 ttatttcatt ttctaacttg ctctcttgct ttctcatatt gttgtctaaa gaatcatgga
     2581 ctaataaaag atataatatt cctaaaaaaa taattatact tatttctttg aaagccttgt
     2641 ttttcaaaaa gttcaccatc tttctacctt ctcgatcttt ttagccattt agccttctaa
     2701 caaaatatgt atattccttt ttattatact ttattttaaa aatattttaa atgtaatcta
     2761 aacactttta atgctaaaag cttcctgtga ttaattcata aaaaacctcc ttttttattc
     2821 aaaataaagg aggtctgtca atcttttata aattaaaata catacttcat aggtgtaaat
     2881 cttatcccat ctttttcttg ttcattatct acaacattaa aagctagctt ttcgtaaatt
     2941 ttaacagcat aaggtgatgc attaacggtt atgacttttt caactttatc gcccaccgat
     3001 aaattctttt taaccaattc gaatagattt ctggctatgc cctgaccatg gaattcttta
     3061 tccacaaaga aaagacatat atgattacac ccacgtacag ctatgactcc aactattctt
     3121 ccttctgtaa atgcaccata aaactccata ttccctgtat taacattatc ttttaaatta
     3181 ttctcatcaa taaacttttt aaaattctca acgccttcag tggaataatc cggcgctaca
     3241 aattctaaaa aaacattcca tactaattct cttgcttcct ttaaatctga aagattaagc
     3301 tttttgtaat gaatcattct tttattcaaa tcctttctat aacattatta attttttata
     3361 aaaactttgc cattaaaata atattgtcgt attttccgtc taactttcgg gccttaaacc
     3421 tttttccttc ataaacaaag ccatattttt cataaagctt aatggcattt gtgttgcttt
     3481 caaaaacttc taattcaatt ttttctatac cgtttcttgt ctttgcatgc tgaatcgttg
     3541 tttcaatcaa acggcttcct attccttttc cccggtattc agcaaggaca cccattccta
     3601 aactaccaac gtgtttcatt tcttcgaagc ttttaggtaa aatatcacac caccctataa
     3661 caatgccgtc ttttaatgca taaaattgag ctaaatcatt tttttctatg atttctacaa
     3721 aagctcttgt agtttccaga ggaaaacctt ctgtcgaggc caaatacttt ttttccctgg
     3781 ctacagtatc gactgcctta caaaaggatt ccacataatc tattttagct tttataataa
     3841 cgatatcatc caccaaatct attccccctt ataataaaaa aatctctcct ttataaccag
     3901 ttagctttat ttttttacta attcttcatg ataaaaataa tttatgtaat taatattatt
     3961 ttgcttgcaa taagcttcta tctcttctga taactcaatc cagtactgct tatcccctcg
     4021 aaaataaatc cttttgtagt cattaataaa ttgaggataa gccaattgaa tgtaatctaa
     4081 gatggttttt ttatagctgc ctctaagatt taaattttca aaccagtact catcaacaaa
     4141 atttttagtt tcttcaatga ttgctttaaa atctgtgata aacgggaaaa taggcgacat
     4201 aaacaaaacc gtatgaattc cttgcttatg caattctttt aaagtagcaa tcctatcctg
     4261 gatgctgctg gcctgatcca ttgcttctct aaacttttca tccagtgtat ttatggagat
     4321 ggatacagtc aaatctttaa tctttcttaa taggtccaaa tctcttaata tcagtttaga
     4381 ttttgtagaa atccctacat gacaattggc atgaactaat tgttctaaaa tttttctggt
     4441 gatttggtat tcttcttcaa acgggttata acaatctgta accgaagcaa gaaacactga
     4501 cttattttct atttttttta aattaacagg cttgtggcat aatttgatat caataaaact
     4561 gccccaatct tccttgtgac ctgtaaaacg tttcataaat gaagcatagc aatacttaca
     4621 agcatgtgta cagccaatat agggattaat aacataatca caatcgggta aattagattt
     4681 tgtcagatag tctttaactt ctacaaattt taggatggtt tccatacatt tatcccctgt
     4741 ctattcaaat cattcagctt accatatatt aaaattgtta cccttcgtct caacgcctga
     4801 ccaaataaaa ataaatgaaa tttacagcaa aagctgataa gaagattgca ttgttttttt
     4861 atagtatctt catactataa taaaactttg ataaataatc cgttaaagat tatcatttat
     4921 gaataaacgt cataacgtct aggatattcc agtctcgctt tttttgtata gctattaata
     4981 aattcagatt tggcttgggt ataagcatcc ctgttaaact catatttttt ccacaaactc
     5041 atttttagca gaccatacgc tttggatact tccggatgat ctatcagata atctctgaaa
     5101 tacaactcat tccagtctcc caaataacga acatggagat gaaaaacttt ctcctcaaat
     5161 ccctctgctg tataaccttt gttgaataca agccacaaac ctggttcgta ctcctcagac
     5221 attaaaatcc aatgattttc ctttaatctt ttgataagct gaaagtagtc acagcattga
     5281 ttcaattcta ataaaatatc tacggtcggt tttgccatta aaccacttac cgcagtactt
     5341 ccaatatgat taatccgata tatatcttct tttttaatca actgaagaag gctgtttttt
     5401 tcactttcgt accattcttt atattgagga ttatgctcct ttaaaataat aggaaataat
     5461 ctccacaact cttctttgct catttctgat aaatcttttt tcatattgcc tccataatcc
     5521 attactttcc taatagccca aggcttacgt ctgctttttt acttatacgg atcaaataat
     5581 actctgatat aatccctctt tattggctgc acatcataaa tttttcactt tgctttcagg
     5641 aaaaactaaa ccataaactt ttcaataaga aattctaata attgtattat tagtagtggc
     5701 attaaaccaa tcgctattaa tacgagtata acaataaata tcaatatcgt cgactcaaaa
     5761 acagacagtt ttcttttttc atttgatttt ttaatctctt cgatgcaatt attatcaaca
     5821 atctcaatat aatcaattgc ctcctcagat tcaggatcta cccagtccag ccaggaatct
     5881 tcagaacaaa ggctaatcat tcgattctga gaaaaggctg attttatcaa aaatattcct
     5941 aattttatca aaccttcctt tgtagcacaa atatatttct gatgccctat ggcaccaaat
     6001 ttaagataaa tgtcttcttg cattaaatcc tcttcgacct tgtccaaaac actaatcatt
     6061 tgttctttct ttgtctcatc cattttatgc ccccttttaa atacacatta aaagttacag
     6121 gctataaata attatctgtt ataaatattc tgtataaata taatgctttt tattggcaca
     6181 atacgttttt tacgaaggat tctctgattt atatgaatta accacgccaa tacctatgga
     6241 tactaagaca agccctaaga aaagcttaat tgtgaagttt tctcctggaa taaacattgc
     6301 tgaaagcaga ctgcccgaaa ctggaaccat aatcttgtaa atagatactt cccctgcttt
     6361 attatacttt aataaagtat taaataatga aaaagctgtc gctgacaaaa atgcagaata
     6421 aataaataaa atccatgctt taggcgtaaa tgtaaaagtt tcaacagaaa ctcttcctat
     6481 gccaaataaa agcagaacaa gagagcccaa aaacatctgc caggctgtta tcacaaaggg
     6541 atgggttttc tttgccagtt cttttgacca aatagaaccc agagcactgg taatacctga
     6601 taaaatcata aaaccttccc ctattactga aaaagataaa gaaaaagtat tttgtccttc
     6661 ttttcccaaa ctggcaataa tcactccacc aaatcctgcc ataagaccaa tgattttgcg
     6721 aaggtttatt ttgtcgttgc tatatataaa atgagcaaaa agcacgacaa aaaaagcact
     6781 gatagaaccc agtacggatc cttttattcc tgatacattg gcaagcccgt tataaaaaaa
     6841 gaagtattgc agagtcgtct gcatgactcc caatccaaaa aaacttaaaa actgcttttt
     6901 gtttatacga agagatattt tcattatcca gtgaattatg ataaacaaca aaacagatgc
     6961 gaggcaaaat ctcattcctg caaaaacaac ctttgcatat aaatcatctg ccttaagacc
     7021 tatctcttca tagcttactt ttagtaccgg aaatgcactt ccccataata gtgaacaaaa
     7081 aatagcaatc agtgatacat aatgcttttt agtaaagaat tttttaatat ccatgttctt
     7141 gtcctttcta ctgttgtagt taaactctcg tcaatacttt tttatttaat tagacttatt
     7201 ttatcatatt atgttgttaa atatatttta tttttaattt ataagattac aagtcacttt
     7261 ataaaaaaag tctaaaaaaa taaaaaataa atcgatgata ttgttgtgac aaacaatcct
     7321 gaaactaaca ctcaggttca gaatgaaaaa tcctcactaa taatgaattt gtaaaataat
     7381 aaaagaaaaa gcataaaaat gaaacgatat ctatcattga catagataaa aaaaaagcct
     7441 cctgggcttt tttaaatttt ttataacata tttattgtaa aaaattgtta aatttattat
     7501 tttagattaa attaaaacga agcttgctca taaagtgcat ctgttttttc ttttgcttct
     7561 cttaatactt caacttgtat aagaagcttc ttatgtaatt tccttgcttt atcaaaataa
     7621 tctttttcat aatctttatg tacataatat tgaagtagat cttctgttat cttcaatgct
     7681 tgattaaatt ctttaataag atttctcata ctggtatcaa gttcttcata cttaggttgc
     7741 ttgttggtaa actttttagc acaattaagt ttatcatatt tttcaaatgt aactgttctg
     7801 agattaaaag gattttttgc ctgataacta ccgtcatcca ttaatttatc cacaattaat
     7861 tcttcatctc ttccagctac ttcaaaataa ttttcaatat taataaaata agtctctata
     7921 tcttgcataa tatatttata agcttcttct ttagattcaa aatgattggt attgtacata
     7981 tgaaatagaa atgcacctcc aattataacg gcagataaaa ctgcaaatcg tacaaaaaag
     8041 cttctttcca tataaatcac tcctcatcta aaactatttt ttgaggcaat ccacactatt
     8101 taataaagtt ataagtccaa atttttataa aaaaaattaa taataccctt ttatagtcag
     8161 attacccttc gcctatttta ccttataaaa aactttttgt aaattattat aagttataaa
     8221 tttttatatt aataaatatt ttccatttga tatttgtttt actcattaaa gtaatctttt
     8281 attcatttat ccgtttaatt tatggaaaag aaaattaatc tctccttttc ttatcttctc
     8341 acgcataaat ttctagatat aattttttta gatcccaaga aactattaat ataaccaatg
     8401 cataaaagga gagattgctt tgaaaaaatt actatatatt accgttaact caaaacctga
     8461 agaattatct gccagtaaaa ccgtaggaag agcttttgta aataaattta taaaaaaata
     8521 tccggagttt tatgttgaag aactggattt atataaagaa cacatcccca gattggaata
     8581 tcaatatttt gaggacagaa attgtgttgt caatgaagaa gccatgaaaa agttgcctga
     8641 aaaagagcaa aaggaaattc aaagaattaa tcaactctgt aatcaattta ttgaagcaga
     8701 tctttatgtt attgcagctc ctatgtggag tttatcattc cctgcccctt taaaagaata
     8761 tatagactgt atcgttcaaa atcataagac cattcatttt cccgaaaaaa atgataagcc
     8821 ggaaggtctc ctgaacgaca aagaacgggc agttatttat atccaatcct ccggtgggaa
     8881 tattccctgg ttaatgggtc ccatctttaa caaaggcatc gattatgtag aagatataat
     8941 gaaactaatg ggaattaatc aatttgaaaa aatactagtc gacggaactg gtacaacaca
     9001 agaagaaaga aagactgcga ttgaaaaggc catggaaaaa attgataaag tcatcgaaaa
     9061 tataaaaatc aaagaagcag ttttacttta attacatttt aaaaggatac tcttattagg
     9121 gtatccttta aatattcttt aatccgctaa aaagcttaaa actttttgtt cgtattcctc
     9181 aggataatcg atacatgctt caatatgtct gctttcaact aaccataatt cctttttatc
     9241 agaagaaatg tttttatata tagcttttcc cattgaagca aagcaaaaat catcttttgt
     9301 gccatgaata actaaagtag gtatcttatt attagtctgg gcagcaacca catctacatc
     9361 ttcaaaagaa aagccataac gatgttttaa ataccaatcc ccacaggtaa cagcatattc
     9421 atatggaaga cctgtgttca tcgattgcca tatgactgaa aaaatgcctt ccatactgtc
     9481 atatgaagag tccaaaatgc aataatctat attttgattg gcatgctctg ttgctgcata
     9541 taagccggcc gttgctgcac ccatagactg cccatgaact acaatctttt tattatttgt
     9601 attctttttt ataaattcaa caaccggaac gatatcattt ttctcgaaat gcccaaaagt
     9661 aacataagga ttttttgacg ttccgctggc acgctggtcc atggcagcaa cattccagtt
     9721 attttgcaaa taaaattcag caataggata cattgacaca tgatctcctc ctgccccatg
     9781 tactagtatg accgtatccc tgtcataatt accgtctgta ctaaaaaaag ttactggtac
     9841 ttcgtttcca tctcctgcaa ttaagtttat cttttcaccc ttgtataaag attcaaagta
     9901 cttataatca tatccccact cttccaattg cttataacta tttgcattcg tatcatttcc
     9961 ctcattttgc ataagcattt gcacagcaat ctcttttcca agataataac tgaatcctaa
    10021 aaaagctatg actaaaatca acataataac aactaatatt tttataagcc tttttaataa
    10081 tctttttcct ttagttttta ctacagtctc cattgattac caccctttgc ttaaggttaa
    10141 acaaatattg aaaatactat tttagttttg gtccttcaaa aggccaaatt cttggataaa
    10201 taccagaaaa acctcccata ttaaaaattt tatgatagaa agcatgaggc tgataaagcc
    10261 caactggtat tttaagacct ctgtataatt catctttttt atcgtccttg ttttttgaaa
    10321 taatttacaa ccattatcca acgattcata gtctctgctt atttttatat atttaagaag
    10381 tggttctcca aaaaccacag ccaaaccaga ttttctaggt atgccattcc accaatatcc
    10441 cataatcgga tgcttttgtg tcatatcatc ctggcataaa accgtgcgag aatggatata
    10501 atcatttaaa ataaagatat ctccataaaa tggctttatc atttcactaa tacgtttaaa
    10561 caatctgatg atttctgttt cccaacctgc catggataat actgctgcat caattgaaat
    10621 ggaagatatc caaagcggta tccaatcgga ccaggaccaa taggatcagg aaccgttata
    10681 taaactgact caacaggctt atgaggatac catatcatat tattatggat attttctgca
    10741 aaaaaagcct caaaagcctc cagactcgta aatttctcct taggcggttc gcaaaaacca
    10801 taacgctttg gtaaaatttc aggaatctgc ctttctatga ttttaagcaa accattaata
    10861 tcttctttat tgagataatg attaaaccac caggacaatt ttactatgga aaatctttct
    10921 gtcttatcta tatcaggaag tcgtttaata cctcttggta aaacaatatc gtttgtttga
    10981 aggtcttcta taagacctat tccatttttt gctaatactt ttgcaacttt atataattct
    11041 tcaatctttt ttacttctgc aattcccggt tcaaggctac attcaatgag aaaccggata
    11101 cctggtaact gattataaat atgttgaggt atgtcttcat catttaagac agcttccctg
    11161 ctgataacaa tttgataatc cttatgaatt aaaataaggc attcctcatt ttcaatcatg
    11221 ttaaactgat tgactaataa attatagtct tgtttttgtg tggtatatat cttcaaatca
    11281 taactcatga tatttactcc tcttatcgta tcgtatagaa tactcgttaa taaagttcga
    11341 aataattata tttattattt ttcagtcact taatttttgt aattagcatt tttttaaaaa
    11401 acatcttatt ccaatacctc ttcattaaac aaaggattct gttttaatta tacgatattt
    11461 tttataaaag gtatgaaatt ttataaatga ataaaaagtt tgtatttttt attctcaatt
    11521 gaccaatgca agatattttt atactctata ggataaaata atgacttttt tctttcagac
    11581 aggtataatt ttattaaaat aatcgaaggg gtaagaaagt gaataaatta gaaaatcatc
    11641 attcaaaccg catatgggaa attgactttt taagaggtat tgctttaatc cttatgatgc
    11701 tttttcattt tttatatgat ttgaatgctt tctatcatgt ggaaatcccc tactgggaca
    11761 gtttttgggt tatagaaggg aaaatatcag ctgtcctttt tatgattctg gctggcatca
    11821 gcagtacctt cagtaaaaat aatttaaaaa gagggcttaa aattttgctt gttgccatgg
    11881 gtataagtct tgttacatat attattttcc cttcagaata catccgcttt ggtatccttc
    11941 atatgttggg aagcagcatg attttatatc attttattta taatatctct ctttcgttta
    12001 cgctgatgat ttctttaatc atttttcttt taggctattt atttaatcat ttttacatag
    12061 ataccaatct cctgattcct tttggaataa tgagcaggga ctttgtatca atggactatt
    12121 atcccttatt tccctggctg ggtgtttttt taattggaac aatcatagga aaaagcattt
    12181 ataaagaaaa aaagagcatc ctccaaaaat tctatagaaa taactttcta tcttttcttg
    12241 gaagacactc tcttatagtt tatcttattc atcagccagt gattttatta ttattatatt
    12301 tctttttttc agtaagaaat taaaaaaagt aaggaaagat tttctttgct ttatataaaa
    12361 caacccaaaa ttatgggttg tttttactta tatatttata ttttaatttg ggtgtaattc
    12421 ttcatccctt aaatctacaa aataatcctc ttgtttttta ttcaaatgat cttgtctggc
    12481 attagcacgt atttcttcca tcttttcttt gtgactaaaa tataatttcg taatcaccat
    12541 cgttgttatt gatgcacatg cgataaatac cattaacaca ctctcaaaca taatctatcc
    12601 ccctagtaag attctcataa ataattacgg ctaaatgaat cataagtatg ctaaataagt
    12661 caaaaaaact ttttacattt tttatttctt aaggaataag aagaagcaag agaaccgtcc
    12721 tcttgcttct ttcaaattag cagtcgtctg ttatgacatc ttcaattgct tttcctggtg
    12781 gcaccatggg cagtactttt tcgtcgatac ctacatgaca gtctaataat actggttcat
    12841 ttaaggcaaa agcttcgttt aaagcctttt ccagttcttc ttcactggtc actctgtaac
    12901 cttttatatc ataagcttct gccagtttca caaaatcagg acctctgtcc aaggtcgttg
    12961 cagaatacct gccttcataa aacatggtct gccactgtct gaccattcct agtgtaccat
    13021 tattaataag tacgataata ataggaagtt tataggcatt aatggttgct aattcattac
    13081 agttcattct aaaacttccg tctcctgcaa tatgtacaac ccttttatct ggcataccga
    13141 tctttgcccc aatagcagca cccgtaccat atcccatagt tcctaaacca ccagaggata
    13201 aaaaggttct tggttttgta aatttataat attggggtac ccacatttga tgctgcccaa
    13261 cttctgttgt aataatcgca tctccttgag tcttttcata tatcttttca ataatggcct
    13321 gaggttttag attgccgttc ttagtataag taacaggata ttcctgcatg agcttttcaa
    13381 ttttttcatt ccactcattg gcttctcttg gttttaatct atcgttgagc atcgtcaggg
    13441 cagtttttat atcccctaag atatagcaaa aagttctgac atttttattg atttccgcag
    13501 ggtcaatatc aatttgcaat attttagccc tgggagcaaa acgttgcaaa ttgctgacaa
    13561 ccctgtcact aaagcgggca ccaattgcaa tcagtaaatc acattccgta gcagcaatat
    13621 tggaagcttt tgttccatgc ataccaatca tacctgtata aagtctgtga gttgccggga
    13681 atgccccctg tcccataagg ctgcaggcaa taggagaatt gattttttca acgaatgtga
    13741 gtaattcctg atcggctcct gcataaccaa ctccccctcc tgcatacaca aaaggtcttt
    13801 ttgcccggtt aatcaattta agtgctcttt caatatcttc ttcacggatt ctttctgaat
    13861 atgcaggaat tggcttaggt tctctgtatt tgtattcagc ttttaaagcc gttacatctt
    13921 ttggaatatc aataagtacc ggccctggcc gaccttcctt tgcaatataa aatgcttttc
    13981 gtatggtatc tgctaattca ttgacatctt ttacaatgta attatgcttt gttataggca
    14041 tagtaatccc tgtaatgtcg acttcctgaa agctgtcttt tccaagcaaa ggtaccgcaa
    14101 cattacccgt tatggctact aaaggtacag aatccatata agctgttgca atccccgtta
    14161 ccagattggt tgcccctggt cctgatgtgg caagacatac accaacctta cctgttgctc
    14221 tggcatatcc gtctgctgca tgggcagctc cttgttcatg agaagtcaga atatgtctta
    14281 tttcattttg atgtttatat aattcatcgt atatgtttaa aaccgcgcct cccggataac
    14341 caaaaatcgt atctacgccc tgttctttta aacattcaat taagatttgt gcgccagtta
    14401 attgcatagc tttcctcctt tgcaaaaaca gggtgcatga acaccccgtt tagtttttaa
    14461 gtattgctcc agtacttgct gaagtaacta atttagcata tctggcaaga taaccgcttt
    14521 taacttttgg ttctggtgcc tgccattttt cttttcttcg ctttaactct tcatcagaaa
    14581 cttctaattc aatggttccg ttaatcatgt ctatggaaat aatgtctccc tcttctacca
    14641 aagcaatggg acctccttct gctgcttcag gagagatatg gccaatggaa gcacctctgg
    14701 ttgcaccact aaaacgtcca tctgtaatta aggccacatc tttgtccagc cccatacctg
    14761 ccaaagcgga agtaggcgca agcatttccc tcatccctgg accgccttta gggccttcat
    14821 agcggataac gaccacgtct cctttattaa ttcttccgga aaaaattgct tcaatcactt
    14881 cttcttcaga attaaatact cttgctggtc ctctatgctg aagcatttct tccgctacag
    14941 ccgatctttt tacaacgcag ccgtcggggg ctaagttacc ttttaataca gcaataccac
    15001 ctgtcttgct gtaaggatta tcaataggac gaataacatt ggggttgcgg tttactgcag
    15061 atgcaatgtt ttctcctaaa gttttacctg ttactgttat cagtgataaa ttcaacaggt
    15121 ttttctttga taattctttc attacggcct ggacaccgcc agcttcatat aagtctacga
    15181 taaagtgagg tcctgctgga gccagtttac aaagatttgg tgttctttca cttatttcat
    15241 tagcaatttc aagatttaat tcaacacctg cttcatgggc aatagcagga agatgcagca
    15301 tactgttggt actgcatccc agggccatat ctacggtaag agcattgtaa aaagcctctt
    15361 tagtcataat gtctctgggt cttatactat ttttaagtaa tttcatcacc atcataccag
    15421 cttttttagc taaacggatt ctttctgcat aaacggctgg aactgttcca tttccaggta
    15481 agcccatacc caaaacttcg gttaaacaat tcatgctatt tgcagtaaac attcctgaac
    15541 atgatccaca gccaggacag gccgtttctt cgtactggta aagttcttct tcagtcattt
    15601 tacctgactt aacagcacct actgcttcaa aaacatcggt taaacttaat cgtttttctt
    15661 ctattttacc tggcagcata gcgccaccgc ttacaacaat agccggaata ttaattctcg
    15721 cagctgccat cagcattgcc ggaacaatct tatcacaatt gggaattaaa actaatgcat
    15781 caaagctgtg agccatagct aaagtttcaa tggaatcggc aattaattct ctggaagcca
    15841 gagaataatg cattccaata tgacccatgg ctatcccgtc gcatacccca atagcaggca
    15901 cttcaacagg tgttcctcca gccatgcgga cacctgcttt aacggcttca gctatggtat
    15961 ttaaatgcat atgcccggga ataatttcac tttttgaatt tacaatccct actaaaggtt
    16021 gttttatttc ttcattggta taacccatcg ctttaaaaag agagcgatgc ggagcttttt
    16081 ccacaccgct ttttgcaaga tcacttctca ctctatcact ccttattcat atataggttc
    16141 gccagtgctg tttgcatatt tttggaatgc ttctaataat ttcttagtga taggtcctgc
    16201 aacaccattt ccaataactc ttccatccac gttcgtaaca gcaattgctt cagcagcagt
    16261 acccgttaag aagcattcat cagcattata aacattaaat aatgtaaatt ctttttcata
    16321 tacttcaata cccatatctt ttgctaaatc aataatggtt cttcttgtga taccatccag
    16381 aattcctata tgaattggag gtgtataaag cacgccatct ttcacaataa aaatattatc
    16441 tcccgtacat tccgctacaa tgccatcatg atttaacatc aatgcttcag gaactcctgc
    16501 acggttggct tcaatttttg ctaagatatt atttaaataa tttaaagatt ttatttgcgg
    16561 gtcaacgatg gttgccttgt ttcttctttg ggatgctgtt ataattggca tacctttttc
    16621 gtacatttcc ggaggatata atgtaatact ggaagcaatg cagaaaacgg aaggtttcgg
    16681 acattttgta ggacttagac ctaaatcccc ttttcctctg gttacaacca aacgaatgta
    16741 cgcatctctt aaatgatttc ttctgcaagt ttcaaggagt acttctttca tttcttcttt
    16801 tgttaaagga atctctaaca taatggcctt tgctgctgca taaagtctgt caatatgttc
    16861 tttgcatttg aaaatcctgc cgttataagc cctgattcct tcaaaaaccc cgtctccgta
    16921 taaaagacca tggtcaaata cagatacctt tgcttccgat tccttaacat attccccatc
    16981 taaataaata attaattcat ctgacatttg atttcctcct tttataaaaa tcctataatt
    17041 cattaataat taactgaccc atttcttttg taccaaccaa tttcatacct tcactcataa
    17101 ggtcccctgt acggtaacct tgttccagta ccttcgttac agcattttct atggcttgag
    17161 cttcctcctc taagccgaaa gaataacgga gcatcattgc tgcagaaaga atcgtcgcaa
    17221 taggatttgc tttgttttgt cctgcaatat ccggggcaga accatgaatc ggttcataaa
    17281 gtccaaagct tccttcagcc agactcgcag aaggtaacat accgatagag cccgtcaaca
    17341 tactcgcttc atcagataaa atatccccaa acatattgct tgtaacaata acatcaaatt
    17401 gctttggatt tctgattaac tgcatagctg cattatccac atacatatga ttgagttcta
    17461 cttgcggata atcctttgca acttcttcaa ccactgctct ccagagtctg gaagattcta
    17521 agatgtttgc tttatcaaca ctcgtcacct ttttatttct tttcatagct gcttcaaagc
    17581 caactcttgc gattcgttct acttctttta cagaataagt ttctgtatca taagcttctt
    17641 caccgtattt ccctgttctt cttcctcttt ctccaaaata aataccccca gttaattcac
    17701 gtactacaag aatatctaaa ctgtccccta taatttctgc ttttagaggg caagcatctt
    17761 tcaatggttt atgaagtaag gcaggcctga gatttgcaaa gagtcctaat tcgcctctta
    17821 aacctaataa ggctttttca ggtctcttat ccccaggaag gttatcccat ttgggacctc
    17881 ctactgctcc cagtaaaact gcatcacttt ttttacaaat atctatggtt tcttgtggca
    17941 gaggctcacc agtcttatct atagctaccc ctccagctaa aacttctgtg aaattaaaac
    18001 gatgaccata tttttctcca attttatcta aaaccaaaat ggtttgctca acaatatccg
    18061 ggcctattcc atctccagga atggttgcta tattaaagtt cattgaatcc gcctccattt
    18121 atcgaatatt ctgttttttg attctaaaaa gttaacacga ataattttta accatttaaa
    18181 gcctaatgag tttcttagtc tggctaatga tgaaacaact attgctctgg cgcaaactaa
    18241 gaaactcaaa ggctaaaaaa ttctataaaa actttataag tcattcattt tttactttaa
    18301 aacttttgtt gaatatattt aatcaagccg tctgaagcaa tgatcttttg cataaattca
    18361 gggaaaggtt gtgctttata agtttcattt tttgttttat tggtaatcgt acctgtggaa
    18421 aaatcgattt ctacttcatc tcctgcatcg atacctttta cagcttcttc acattcaagg
    18481 ataggaagtc cgatattaat cgcattacgg taaaaaattc tcgcaaaagt gctggctata
    18541 acgcaggaaa ctcccgaagc ctttatggca attggcgcat gttctcttga agaaccacag
    18601 ccaaagtttt tatccgctac aataatatca ccttttcgca ctttacctgc aaactcagga
    18661 tctatatctt ccatgcaatg ctttgctaat tcctgtggat cagatgtgtt taaataacgg
    18721 gctggaatga ttacgtccgt atctacgttg tctccatact taaataccct tccttgtact
    18781 ttcatcgtaa cgcctcctta ttctaaatct tccgggtttg tgattttacc agtaatagcg
    18841 gatgctgccg ctactgccgg gcttgccagg tacacttcag attcaggatg tcccatgcgg
    18901 ccaacaaagt ttctgttggt ggttgaaaca gctctttctc cttttgccag gatacccata
    18961 tgtcctccaa gacaaggacc gcatgtaggc gtactaaata ccgctcctgc ttttacgata
    19021 tcttccacta aaccttcttt taaagcctga agataaatat tttgtgttcc tgggaataca
    19081 atacaacgta cacctttagc cacttttctg cctcttaaaa tttttgctgc aactcttaag
    19141 tcttccatac gcccattagt acaagaacca ataaccacct ggtctatttt gatatcccct
    19201 acttcatcaa tggttcgggt attttcaggt aaatgaggaa atgctacggt tggacggatt
    19261 ttacttaagt ctatttcata taccttttca tattccgcat cttcatcggc ttcataaatc
    19321 ttatattctc ttgtggaatg ttctttcata tattcaatcg tcttttcatc tacagggaaa
    19381 ataccatttt tagcccctgc ttcaatagcc atatttgcaa tggtaaaacg gtcatccatt
    19441 gttaagcttg cgataccatc tcctgcaaac tccatagatt tatacaatgc cccgtctaca
    19501 ccaatcatgc cgataatatg aaggataacg tcttttccac ttacccattt tgacattttt
    19561 ccttttaata caaatttcag ggcagcaggt actttaaacc atgccttacc cgttgccatt
    19621 cctgcagcca tgtcagtact tccaatacct gttgaaaaag ctcctaaagc accataagta
    19681 caagtatgag agtctgcacc aataacaaca tctcctgcta caacaagacc tttttcggga
    19741 ataagggcat gttcaattcc catttctcca acttcaaagt aattttcaat tcctttttca
    19801 taagcaaatt cacggatgaa tttacattgt tctgctgcct tgatatcttt attaggcgta
    19861 aaatggtccg gcacaatggc tactttactt ttatcaaaaa ctttatcaac ccctattttt
    19921 ctaaattctt ttacagctac aggggtagtt atatcatttc caagaactaa atctaaattg
    19981 gcttctatta attgccctgc tttaacactg tcaagacctg cgtgggccgc taaaattttt
    20041 tgtgtcatcg tcattcccat agtatcatct ccttgttaca aatatgcttt aaactttttt
    20101 tcgatatctt taattaattt atactcaatt gaatccacta gagctatcca actggcttcg
    20161 ataatgtcag aagacacgcc tactgtggtc caaatatcct ctccatccgt agattcaata
    20221 agaaccctta ccttcgcagc agtcgcttgc ttggtgtcta aaactctaac cttgtagtcc
    20281 gtcagatgaa catctcttaa ttcaggataa aagacttcta aagcctttct taaagctttg
    20341 tctaaagcat taacaggacc atccccttct gctgctgtaa tttctgtctg tccatccact
    20401 ttcactttta tcaaagcaga agagctaaag ctttcttctc ctgccggatg ttccccaata
    20461 attttaaaat gctccaactc aaagaaaggt ttatatttcc ctaattcctt tctgataatg
    20521 agttcaaatg tgctttcagc cccttcaaat tgataaccaa tatgctctaa tttctttaat
    20581 ttatcaatga tatttttagt ttccggagaa tctttatcaa tacctgcatt aaacttttgt
    20641 atcttagcta aaatggtgct tctgcctgcc acttctgaca ttaaaaatct tctttcattt
    20701 cctacgagtt ccggatcaat atgttcaaaa gaatggctgg ctttagaaac accatcaata
    20761 tgcataccgc ctttatgggc aaaagcactt tttcccacat aaggcatccc gtccgttaag
    20821 ctgacattag ctatttctgc tattctcctg gcagtctgag ttaaagccac cattttatca
    20881 tcaggaatac actgataatt cttttttagt tgaagattag caattaccgt tgacaagttg
    20941 acatttccac aacgttcacc aataccaata aaggttccct gaacatgtga ggcacccgcc
    21001 tttacagcca taatcgcatt ggcaaccgcc attcccccgt cattgtgggg atgaatacca
    21061 acatgcagat taaagttttc tacaactttt ttggttattt catagacttc atccggaaaa
    21121 gcacctccat tggtttcaca aagaacaata cagtctgctc ctccttctac agctgctgaa
    21181 agagttgcca tggcatattc aggatttgct ttatagccat caaagaaatg ttctgcatca
    21241 aagataactt ctttaccttt ttctttaaaa aatctcaaag tttctttaat cattgccagg
    21301 ttttcttcta aagttgtttt aataatatcg gtcacatgaa aatcccagct ttttccgaat
    21361 ataaccacta cgggagtatc tgctttcaaa agggattgaa tattattatc ttcctccaca
    21421 gttgtatctc ttcttctggt actcccaaaa gcagctaacc tggcattctt taagctcata
    21481 ttttttactc tctcaaaaaa ctccaaatcc tttggattgg agccgggatt acctgcttca
    21541 atgtaagcaa cccctaactc atccaaagtt tcaactattt ttaatttatc tgcaactgaa
    21601 aaagatatgc cttctccttg cgccccatcc cttaaagtag aatcaaaaat tgcaatttgc
    21661 tttttcattg taaccaccac tccattttat ggttaactta tttagactga atccaactca
    21721 tcattttacg aagctctctt ccaaccactt caattggatg ttcttgttcc atctttcttc
    21781 ttgcattaaa tgaaggacgg ttggcttgat tttcaagaat ccagttctta gcaaaagtac
    21841 catcctggat ttcttttaat acttttctca tttcattttt tgtttcttca gtaataatac
    21901 gttttccaac actgtaatcc ccatattccg ctgtatcact gattgaatat ctcatatagc
    21961 ttaagccgcc ttgattcact aaatcgacaa ttaatttcat ttcatgcata cattcaaaat
    22021 atgcactttc tggttgatat cctgcttcaa ccaaaacttc aaaaccagct ttcatcagtt
    22081 ctgatacgcc accgcaaagt actgcctgct ctccaaaaag gtctgtttct gtttcttctt
    22141 taaaggttgt ttccagaatt cctgctcttg caccaccaat accagccgca taagcaaggg
    22201 ctaaatcttt agcattaccg gttgcatcct gataaactgc taccagacat ggaacgcctt
    22261 ttccttcaag gaactgactt ctaactgtgt gccctggtcc ttttggtgca accatgaaaa
    22321 cgttaatgtc ttccggaggt acaatttgcc caaaatgaat attaaatcca tgagcaaaag
    22381 ctaaagattt tccaggactt aaattaggtt caatgctttc tttatataat ttgggttgtt
    22441 tttcatcatt aattaaaatc atgatgatat ctgcttcttt tgcagcttct gctgctgtag
    22501 ctaccttaag accagcagct tcagcctttg gccaggattt gcttcctttg taaagaccaa
    22561 caatgacatc aacacccgat tcatgcaaat taagagcatg tgcatgacct tgactaccat
    22621 aaccgataat tgctactttt tttcctttta ataaagataa attacaatcg tgttcatagt
    22681 ataattttgc cattttttaa ctcctccttg tatttattaa ttttattgat taattgtgtt
    22741 cttttataac tttattgcct cgctccaggg ctgtaagccc ggtgcggatt atttctttaa
    22801 taccataagg ttccagcata ttaattaatg cctcaatttt agattcatcc ccagttaatt
    22861 cgatgatcag ggaatcgtta accacatcca cgattcttcc tctgaataca ttgacaattt
    22921 ctataatttc tgctctttgc tttgttgttg cagagacttt aattaatatc aattctctat
    22981 aaacagatga ttcgggtttt aactcaataa ctttaatcac atcaatcaac ttgtttagtt
    23041 gctttttaat ctgctctacg atgtatttgt ctccttttac aacaatcgtc atacgcgatg
    23101 tattaggatc ttcggtcaca ccaacagtaa gactgtcaat attatatcct ctgcggctaa
    23161 ataagccaga tactctgctt aatacccctg aatggttttc aaccaaaaca gataaaacat
    23221 accgtttcat attatccccc ttactcttaa aaatctaaga atttattaaa gtgtactttc
    23281 tttaggatct acaatgcatt caattaagaa agcagactca gtattcattg ctttggtaaa
    23341 tgcttctttt aaatcttcat tactggaaac tcttatccct tctatgccat aagcccgggc
    23401 taactgaata aaatcaggat tactgcttaa gtcaacagca tattcagctt cataaaattt
    23461 gtactgcatt tccctgacca tacctaaacc catattatta aataggagaa taattaattt
    23521 taaatgattt tgcttaattg tggctaattc ataaaggctc atttggaaac caccatctcc
    23581 tacaactgca acgatctgcc ggtttttatt tgctatggca gaaccgatgg ccgcaggtaa
    23641 actatagccc atggttccca atcctccaga aataaagaat tttctgtctc ctgaaatttt
    23701 aaaatttaag gcagcccata tttggttttg ccctacatct cctactaaga ttgcatcatg
    23761 attaagcaca tctgataaaa gttcaatggc atacttcgga ttcacatact ccttattcgt
    23821 tttaatatct tctttaaatt ttttatgctt ttcttttaat tcccaaagtt ccctaagcca
    23881 gtcttctgtt tgtagaggct gggccatttc cagtaattgt ttcaaaacgt ttttggcatc
    23941 tcctacaatt ggaatcgaag ttcctagatt cttaccaatt tctgcaggat caatatcgat
    24001 atgtatgata tcaggttcat ttttaaataa tttattctca cctgtagccc tgtccccaag
    24061 cctcgctcca ataaacataa tcacgtctgc ttcagctaca gctctgttgg cataatgatg
    24121 tccgtgagaa cctatcattc caatatagta agaagaatta tggtctattc cgcccagacc
    24181 cattagtgtc gatacaactg gaattttggt tttttcaaca aagtctaaaa gttccttttc
    24241 tgcatgagaa ctgatgactc ccccaccaat acatataatg ggttttttac tttctttcag
    24301 ccttgtaaag gcttttttaa cctgacctgc atgaccaacg taagttggtt tgtaacctct
    24361 gatattcact tcttcatcat aataaaaatc tatatcttca acaagaatat ctgaaggaat
    24421 atcaattaat acaggtcctg gcctcccagt ttttgcaata taaaaagctt cttttattat
    24481 tctgggaata tctttggcat tttttatgag atagctgtgt ttcacaaaag gttctgtggc
    24541 gcctgtaata tcgacttcct gaaaaacgtc ccgcccaatc aaagtagact ttacctgacc
    24601 agtaattgcc actatcggaa tagaatccat ataagccgtt gctatacctg taattaaatt
    24661 agtagctccc ggaccagatg ttacagtaca tacccctact tttccagaag ctctggcata
    24721 gccactggca cagtgagcgg cagattgttc ctgacgcact agaacatgct caatttccga
    24781 tcttcttaag gcatcataca agggcatgat ggcagcaccc ggatatccaa atacgcaaga
    24841 cacattttct tttctaaagc attctactat ggcatctgtc gctttcaccc tattcctcct
    24901 tactaaaatt ctaaacttga gattattaat tgattataag cactttttat cctatttgta
    24961 aataatcaac gattttccca gatataaaaa acctcgtccc cacatgtagg gacgaggata
    25021 tactccacgc gttaccaccc ttattaactt cattttacaa taaagttcac ttactaggtt
    25081 ccaacaaacc tttgcctgta acggggcata ccgtttatct tctactcata gccaaaggct
    25141 atatttcgaa gaaactgctc aggagggatt catattttgc gataggatcg gttcgcacca
    25201 accaccgact ctctgcacct atctggtgca aaatatcagt ctccatcatt gcatttttct
    25261 gaaaactaaa tttgtaagta ttgtagcagt atttaaaaat attgtcaata actttttgat
    25321 aaaactaaat cacttttatc aataaaattt ctatcttgaa aaataatctt ttcaatttct
    25381 tctaatggta atggcatact aaataaatag ccttgcactt tatcacattt ctggtatttt
    25441 aagaaattaa actgttccca tgtttctacc ccttcagcag taacatatag ttccatatta
    25501 tgagataaat caataactgc ctttgcaata gtttgttggt taatgtcatt agtgatatta
    25561 tcaacaaagg tcctatcaat ttttactgta ttaattggta gttgttttaa gtaattgagt
    25621 gaagaatatc ccgtcccaaa atcatccaac gaaatattga ttttcttttc ccgcaattcg
    25681 ccaagtactt ttttcgttaa ttccagatcc ctcatggcca cactttcagt aatttctaaa
    25741 tcaagaagtt tgggatccat accagtttcc tctataattt ctgtaatcat atccactaaa
    25801 tctggctgtt gaaattgcct tgccgacaga ttcacggaaa tacggagatc tttagccccc
    25861 ctcttctgcc attttacact ttgattgcaa gcagcccgga taacccattc accaattgga
    25921 acaattaatc cagtttcttc agctaaaggg ataaactcta aaggagaaag acagcctttt
    25981 tcaggatgca tccagcggat taaagcttct actcctacca tttcaccagt cataagatcg
    26041 atttgtggct gataatataa gacaaattcc tgattaatca atgcttgtct taaatcctct
    26101 tctaatttat acttttccaa taatttagta ttcatgtgcg attcaaataa acaatagcca
    26161 ttcttaccat tttcttttgc acgattcatt gccatcgttg catattttac aatcgtataa
    26221 atgtcatccg aatcatcggg ataaatggca attccaatac ttgctgtaat ataaaatttt
    26281 ttctcgtaag aagtaaaagg aatctgaagg ctttcaataa tgcgttccac cacactgatg
    26341 acgtcatctt tattattgat gccaggcaat agtataacaa attcatctcc ttcaaaacga
    26401 catacaaaat cactatgtct taaacacttt ctaattaaat gtcctatttg ttttaataat
    26461 ctgtcaccta atgcatgtcc gatggtatca ttgacgactt taaaattatc caaatcaata
    26521 taaattaaag cagctttata attgtatttt tttacttctt caacaaagtc aggtaagata
    26581 tcttcgaaat attttctgtt gggaagatca gttaaactat caaaatacgc cagctgaaaa
    26641 atcttttttt gagatttttg ctgtgacatt ataaaggcaa taataattgt aaaaatgatg
    26701 agctctaaag ctaaaagaac gaaaaaaatt aatgagctac tggatttatc ttcatagaac
    26761 aaattgttat tccagttaaa tctcattatt actgtaaaaa acatacttat taatagtaaa
    26821 agaattccta gggttgtaat tttatagtaa gtattatcga gaagaaacag gcctcttttt
    26881 gaaaactctt taaatttctt tttttcctta ttgttccact tgttatccat tttcacatta
    26941 tcaactccat ttggcccgac gttatcatag aacaaacaaa agcaacatga ccacaaatta
    27001 tgctattatt attatatacg aatattttgt cgatgtaaac aatgcaatta tcttaattat
    27061 cataatgcat tatcttttat ttaaactttt aaatattctt aattattatt ttcctgccaa
    27121 taatatcaat ttctcctgct tttttaagtt cactcatcac ccggcttgct gtttcccgtg
    27181 ttgtaccaac gatatttgca atatcttgcc tggtaatgtc taaattgagt tctatccctc
    27241 tgcttgtttt tattccatgt tcttttgcca gatttaaaag cacatttttt gttcttaaat
    27301 atacattggt caaagccatt tcttttacat tcatttggga atggtataac cttttggtta
    27361 aaacttttat aatctgcaaa gccaaatctt tgtggcttgc aactaaattt tcaatttcac
    27421 gattatatat catccctaaa accgcatctt ctatgacttc tgcagtcgct ggatagtcta
    27481 tatcattaaa catggtcact tctccaataa cagagccttc tccaaaaatg tttaatataa
    27541 tctcttttcc ctcatcatca gttttataca tttttacctt acccgataaa atataataaa
    27601 aggcatctcc cttttcacct tcaaaaaata tcactgaacc ttttttatat tcttttctta
    27661 tttccagctt tgcgatgtct tttaattgtt cttcagaaag ctttgaaaag actaatattc
    27721 tttttaataa ctcaatattt ttctccataa aatccctcgt tttactatca gtttactata
    27781 atatatagtg tacaatattt ttttttataa atcaaatagg tataatataa atgaaattaa
    27841 ttccatcaaa acaacaaaag actgcataat tttatgcagt ctttattgat ttatacagtt
    27901 gttttttcaa cctttccaaa gactttttca ataaattcat gactcatttc ttttcctaat
    27961 acacttgcta aaggtaccgt aataaaggat accagcatag caatggtaga aacaattggc
    28021 atatcaaccg ttaatatacc aaaagtttgc aaaatgatat agccgccaat cacaatacct
    28081 ccaccgataa aacctgccca ggcgccaaaa cttgtaactt ttctggacca gattccccaa
    28141 acgtaaggtc ctaagaacat cccagaaaca acaccccagg ataaggacat taaataaaca
    28201 atggtattgg attggaagat cgccataatg tatgataaaa gaacaaaaat aatacaaaag
    28261 aatctcatat aataagtcgt cttcttatca gataaatcat tttttacaaa accctttata
    28321 aaatcaatgg aaatggttga gctcgaaaca agcactaaag cagcaagggt tgacatagaa
    28381 gctgaaagaa tcagtacaat aataatacca agtaaagcat ccggtaaagc tttttccaac
    28441 ataagcggta caatcatatc tgcgttgttt tgaggcataa ccgctacacc attttctata
    28501 aaaaacagac gtcctaatgc accggtaaaa taggcactaa ccccaatgat aaaagcaaaa
    28561 agcgttgaaa taatggtacc tcttttaatc gctgcttcgt ctttaatggc ataaaactta
    28621 tgaaccattt ggggtaagcc ccaaacacca aaactcgtca taaggaccat gctcattaaa
    28681 ggtatgaatt gagaagcatt gggaataaaa gaaccagaac ctggttcaag gtctgacaat
    28741 ttacttaagg ctgcactaaa ccctcccact tcagggcttt tcagcacaaa aaataccatc
    28801 aaaatactgc caataatcat aataattcct tgaataaagt cattgatagc ggttgccaca
    28861 taccccccca agataaggta tacagcggtt aataaagcca tgattaaaac cactgtatta
    28921 aagcttatac caaaggcgct ttcaaagatg tagcctaaac ctttatatac ggaagctgta
    28981 taaggaacta aaaagataaa caccactaaa gccgctataa acttcatatt tttactttga
    29041 tatctctttt caaaaaactc cggcatggtt ctggcatcca aacgctgtgt tatatcacgg
    29101 gtccttctag ccaatagttt ccatgcaagc cagcaaccaa aaacagcatt gccaatccca
    29161 atccaactgg ttgaaacacc aaatctccag ccaagtcctc cggcatagcc tataaaaatt
    29221 acagctgaaa aataagaagt cccataagca aaagcactca tccaagctcc catgcttctt
    29281 ccgccaagaa agaaatcatc gctgcttttt atcctggtct tactatatac accgactatt
    29341 atcataacca gaataaaaag tagtaaaaag gccaacttca tttcataccc tctcctttta
    29401 aatctaaatt aaaaaaactc ccgtcctttc ttaaaggacg agagtcagct cccgtgttac
    29461 caccttcatt cgctgttgtt tcacaacaac aacctcttca agtacaccat atctaactta
    29521 tgggatactc tagcactata acgggtgcat tccgttttcc cctacttgga tgccctttca
    29581 ggaaaacact cgaagatgta ttcgaattaa tacactttgc cccttccacc agccgggtgc
    29641 tctctgtaaa agcttaatta attctacttc ttcttctcat cgctttaata agttcgattt
    29701 attttcttca ttatatgatg tctaaactac cttgtcaata gccttctcct atgaattgac
    29761 taaatcaatc aaaactaaca aattttaata attgttttta tttataatca ttatctaagt
    29821 taatcacaat agcttttgat ctagtcatgg cttcaatact atatcgaatt ccctgagtcc
    29881 caagaccgga agccttaacg cctaaaaacg ggaaatgatc cggacctctt tcagttttat
    29941 tattaatctg cacagtgcca acttccagtt tctgtgcaat agcaaacgct ttatcgatat
    30001 ttttagtaaa aacacagcct tgaagaccat actcagactt gttagaaata tcaatagctt
    30061 cttctatact ctttacacgc ataaccggta atactggtcc aaaaggttct tcccatgcca
    30121 gccgcatatc ttctgtcaca taatcaaaaa ccgtcggcca gatatatctg ccttctctct
    30181 tattgcctat taaaagtttt gctcctttat ttatagcatc atcaattaat tcctgaacaa
    30241 agtctgcagc cttatcactg atcagcggtg tgatatctgc atcctcttca ggtttaccga
    30301 cttttagttt ttttacctca gccaccattt tagcaaccaa ttcatcagca atctcttcca
    30361 tcaccaaaac tctttttact gccgtacacc tttgtccaga ataattaaaa gctcctttta
    30421 caatattttg agccgtttca tccaaatcac agtcttccag gactatagct gcatcttttc
    30481 ctcccaattc caataagaga ggaatcatgc ctgcttcttt agcaatatgc tgacccactt
    30541 ctgtactgcc ggtaaaatta atcatatcaa taccaggatg agtaatcaga taatcgccaa
    30601 tttcagagcc cttacctgta atgatgttta aaacccccgg tggaattccg gcttcatcga
    30661 aaatatgtcc cagatacata gcgctaatag aaccctgagt gggtggttta aaaataacac
    30721 tatttcctcc cactaaagca ggtgcaactt tagataccga taaatttacg ggatagttaa
    30781 atggggatat gcataaaata acccctaagg gaacccggtc tacaatcgat atttttgttt
    30841 tatcaaaacc aggaaatgca tccccataaa tcgtttcccc agtcatccgc tttgcggttt
    30901 ctgctgtaaa atgcataaaa tctatggttc ttttgacttc cgaaatcgct gaactttttg
    30961 acttggcaat ttcttttact aaaatattcg ctatctcttc aatgtatttt tccattattc
    31021 ttgcggcttt gtgaataatg tctgcccggt ctctaatcgg cataaaagcc catgattttt
    31081 gtgcttcttt agcacactca atcgcataat ctacatcatt tttagtttgt gcctgtattt
    31141 ccccaattaa tgaatcatct acaggagaat agattttaat tctttgcttc ttttctgatt
    31201 ctacccattg accattacat aaatttttat aaacattcac ttctccttct ttatatacaa
    31261 aagaaaacat cctatcacct ctttcttcta tcattctatt ctttccatta atttaaattc
    31321 atatccagtt gcttctattt gttcttttgt taatacactt ccttcaggcg gtcttttttt
    31381 cagtttaaat accactgctt tttgccctat ttcctgctta aaatctatcc ggttcatagg
    31441 tatatttatg cccaataaat cagacattat ttctgcagtg gctttatgcc ctatagcaga
    31501 aataaaacca ttttcttcaa ttaatttttt cgcatcatct acagaaatgc cttgcactct
    31561 gtataatcca tctgttgtag caaccgttcc attaaataat gctatcggat agtttttcat
    31621 aaaacgctcc atccgccttc ccaataattt tttactagtt taacctaatt caaaattttt
    31681 tacaatatta ttttattttt attaataaga acttgtatat aataattttt ttctataaaa
    31741 tataatcaat atcagtcata aaggagggac cctgctgtgg aaaatgtaat gtttgctttt
    31801 ggaataaccc tctttgcagg cttagcaaca ggaatcggaa gtatcctggc cttctttgct
    31861 aaaaaaacaa atacaaaatt tttgtccgtt gctttagggt tttctgctgg tgtaatgatt
    31921 tatgtctcta tgatagatat ttttacaaaa gcaaaggatt cccttgttct tgttttaggt
    31981 gagacaaaag gctcatggat tacagtcatc agcttttttg gcggaatgtt tttaatcgga
    32041 ctgattgata cctttattcc ttcaacagaa aatccccatg cagtgcatac cgttgaagat
    32101 atgaaccaac ccctttctcc caaaaaggat gaccttttcc gcatgggtat ttttacagcc
    32161 cttgcaataa ctattcataa ctttcccgaa ggacttgcta cttttactgc agcaataaaa
    32221 gatccttccc ttggcattgc aattgctgtc gctattgcta ttcataatat tcctgaaggc
    32281 atcgccgtat ctgtgccaat ttattttgct acagggagca agaaaaaagc ctttatttat
    32341 tcttttttgt ccggactttc ggaacccctg ggggcaagtg taggatttat cctcttgtca
    32401 ggcttattta acgatttaac ctttggaatc ctgtttgctg ccgttgcagg aattatgata
    32461 tatatttctt tagatgaatt attgcctgct gctgaaaagt atggggaaca tcacttatcc
    32521 atatatggtg ttatagcagg catgatggtg atggccttaa gtttgttatt atttatttaa
    32581 aaagcaactg catcacagga tgcagctgct ttttgattat cagcaatcta ataatgatta
    32641 aatatctact tgtaaaagct ctatggcttt acatgaaaac tctatcgttc cattttcata
    32701 atcccttact aaatatcttt gttccagatc ccaacctaaa aacaatcggt caataatctc
    32761 aaatcccatg atttgattat atttacctcc aatagagcgc atgattaagt tatttattcc
    32821 attaaattta aaacaggctt tcattaaacc atgattggat aattccagcc caaataatat
    32881 ttttaaataa gagcttcttt ctccaaattc atttaaatct atataataat taaaagcttc
    32941 tatgtacttt attttcttcc aggaaaactg cggatgaatg cttaatattt tctccatccc
    33001 tgtaatatct ttataaagtc tgttctcgtt aatcatagca tgcccctttc tttttcaagc
    33061 cgaaaaatct ctcatcattt catattcgat atagaataag gtttttggaa acagcttatt
    33121 cattttttat tattatacta tagtaattat tgaaaaaaag tctaattttg tttaatcttg
    33181 atttaaaaag caccttttat cataaaatga caaaaggtgc tttttttaaa attaaaagtc
    33241 tttatcctaa aaacattttt aaatcatctt ctacgtttgt aattccgcca ataccaaatt
    33301 gttctactaa tacttttgcc acatttggag aaaggaaagc tggaagcgtt ggaccaaggt
    33361 gtatgttttt tacacccaga tataaaaggg caagaagtac gatgaccgct ttttgttcat
    33421 accatgcaat attataggaa attggcaatt cattgacatc atttaactca aagatttctt
    33481 ttaatttaag cgcaataacc gctaaagagt aagaatcatt acattgtcct gcatcaagaa
    33541 ctcgtggaat accgccaata tcacctaaat ctaacttatt atatcggtat ttggcacagc
    33601 ctgctgttaa aataaccgta tcctctggaa gagatttggc aaattctgta taataatctc
    33661 tgctcttcat tctgccatcg caacctgcca taacaaagaa gcgtttaata acacctgatt
    33721 ttaccgcatc cacaatttta tctgctaagg aaaacacagt attgtgcgca aatcctccta
    33781 taatttctcc cttttctatc tcaacaggtg ccggacatct tttagcatgt tcaatgattg
    33841 ctgaaaaatc cttctttccg tcttttccgg cagagatata ttgaattcct tcaaaaccta
    33901 ctgaacctgt tacataaact ctgtctttat aagattcctt aggtggtacc agacagtttg
    33961 tggtcattaa tatcggacca ttaaatttct caaattcctc attttgtttc caccatgcgt
    34021 tcccatagtt cccaacaaaa tgctcatact ttttaaatgc cggataataa tgagcaggca
    34081 acatttctcc atgagtatac acgtcgaccc cagtatcctt tgtttgctca agcaattgtt
    34141 ccaaatcctt caaatcatga ccactgatta aaatagctgg attgtttctt acaccgatat
    34201 taactttggt aatttcagga tgaccataac ttgaagtatt ggcttcatca agaagagcca
    34261 tgacatctac accatattta cctgtttcta aagtaagtgc taccaaatca tctatggtta
    34321 aatggtcatc taaagtagcc gctaaagctt tctgaaggaa agcatgtatt tcatttttat
    34381 catagcctaa attaatcgca tgtttcccat aagctgacat tccttttaaa ccatatgtaa
    34441 ttaattctct taaggatcgg atatcttcat tttctgtcgt taatatgcct acggtttcag
    34501 cttttttatc aaactcttcc ttgcttactg taaaaattgc agcatcgtgg gtaatctttt
    34561 caacctttcc tcctgcttct gccactgctt tttttacatt ttcacggact tctaaagctc
    34621 tttcaattct atgaatgaaa tcatttctgt caaaatttgc attagttatg gttgaaaaca
    34681 tattttccat gataaattca ttgacttctt tactttcata gccaacttta cgaccttccg
    34741 ttgtatacat tgaaatcccc ttaaggacat agataagtaa atcttgcaga ttagctacat
    34801 ctgcactttt tccacatact cctcttaaag tacagccttg tccttttgct gtttcttgac
    34861 attgataaca aaacatatta ttttcctcct tttaaaattt aattaaaccc acaggtttac
    34921 tttgtttaaa tatataataa gtcataaaaa aagaaaaaac cgtgacataa atcacggatt
    34981 gaaaattttt tattttattc gccaaccgtt ttcctgtaat ctaatatctt gggcgcctta
    35041 ggtgccctga ttctatgagc aacaggaaaa ttcgaaccaa ccagaggact tagataatat
    35101 ttaaatgcat cggtaacatt attcccctct ttattaataa aatgatccgg catgactctt
    35161 gtcttgcctg caatttcttc taatctcctc aattcatagt ttactgaata aaaacctgtt
    35221 cgctgaatag taatggaacc atcgacgtca taccagatgg caaattgcgc tgctttttcc
    35281 cctacttctc ttgcttcatt ctggtccaca tctgaaacac aacctataaa agagcgctgt
    35341 aaatagccta aagtatcaga acgcaccctg ttaattttca atttgtcttt tacacacttt
    35401 gccagcaagt cccctaaagc acctgtcccc gaaagctgca catttccatg agcatctttt
    35461 tcaacatctt ccattaactt tgttataacc ggaacaccct tttcatcctg gattccttct
    35521 gcaactgcaa ccacacagcg tccatactgg taatatacct ttcttacatc agacaaaaat
    35581 ttatcgatac taaatggtct ttccggcaga taaatcaaat gaggtccatc gtcaggatat
    35641 ttttgcgcta aagaagaagc tgctgtcaaa aaacctgcat gtcttcccat aacaacacct
    35701 atgtaaaccc ctggcaaagc tcggttatca agatttacgc ctataaaggc ctgagctaca
    35761 aatctggcag cagaaccata acctggcgta tggtcattta aaaccaaatc attatcaatc
    35821 gtttttggaa tatgaatggc tctgaattca taatcggatt tttttgcatt ttcattaaca
    35881 atccgtacag tatctgcaga atcattacca ccgatataga aaaaatatct cacatcatgt
    35941 gcctttaaaa catcaaagat ttccttgcaa tacttttcat ccggtttatc cctggttgaa
    36001 agcagggccg atgaaggtgt acgtgctacc tgctctaaat tgtttgtggt ctcttgtgtc
    36061 aaatccacaa aatcctcatt aacaatgcct gcaaccccat tgactgcccc atatactttc
    36121 gttacttgct gaaatttcct ggattctaaa accactccca caagagactg gttaataaca
    36181 gcagtaggtc ctcccccttg agccacaacg acttttccct gcagcttcat aaaattcccc
    36241 cttttatatt ttccattttt tgtatattat aacatttatt taaatgattt tccattgaat
    36301 tttttattat ttctgtatta tttcagtaaa ctttattaaa taaaaatatt agataagcat
    36361 tgaaattaag atattaaaat tatatgcttc taaaataata ccctattcct tgaaaggaat
    36421 agggtagtta ttatttcata ctaattttaa tttaactaaa aatttaataa aacaaaattt
    36481 ctcgacagtt gttacgataa aatatatact ttttacttga catactatta ttttaatata
    36541 aggagaggaa ctatagcatg agaaaagatt cattattcat tcttactatt atgctaacat
    36601 taacttaagt atgtgcttgt tcttctaaaa ggctgaagac gcaaaaagca caaacagcaa
    36661 tatttcagga gaaatacaat caaattcagg atcaaaaaat gaatccaata aaatcactga
    36721 tttaaataca gaaaaatcaa gtgaaaaccc ttcaagcgag aaagaatatt atcactgatg
    36781 gaggtaaaat aattggattc ccacgagcat tataaatgct ctgctaataa taatataagc
    36841 aaaaaatgca gttttaacaa aattcttaaa ggtaaaagcg ctaatattaa ttacaatgga
    36901 gatgtaattg ttttaggttt tcgtggaaaa gattatttat tacaactgtc ttaagcaaat
    36961 taaaatcttc tggatgttta tttcaaatta aattaatctt ttaggaggaa ttaaaaatga
    37021 attcttttaa aaaatgcatc tgtttattaa tcagcacttt attattaatt agttcccttt
    37081 ctggctgtgg cattcttaac aattcaaaat caactggtag ttcacaaagt ggaaatactg
    37141 tttctgaaga tacagataat aaaaaacaag aaaaacaagt tggtgccttg ataatcctgt
    37201 gcccctgggt gagtcttttt cctgggaaga tgaatattcc agtgatattt tagatcctat
    37261 agcttataca ggaacctata cagcctgtat taaatctgta aaaaaattag aagacagtga
    37321 atatgaacag tatgacttag aaaaagatga aaatgcagac gcttacttaa tttccattga
    37381 aatgtccgga gatatcacac caactgtagg aaaggtatct tacaactcta ccattccaaa
    37441 tatttctggt acaagagtac cagaaggcgg tggatatatg ggcgctgtaa ccagcggatt
    37501 tgatggttct atcagtcgag taatatctga caaatatggt ttatccactt tactgaacaa
    37561 caatgaaaat aatcaggttg attacagtgg agaattcatt gtttatggtt ataaagaata
    37621 tgaaaactta ttgatgataa aagtgcaaaa aaaatatctt gatgaaaatg ttccatctga
    37681 cgaaaaatat atttacttta aattgaaata gtatattatt ttatataact tatggcttgc
    37741 ctgcccatct gtttccagga agcataaaaa tccttcctgg aaactttttg attgggcgca
    37801 ttttttaatg gattatttat ataaatataa tccttatcat aacccgtaat caaaacagaa
    37861 tgttctttca tcgtaatccg tatttttcca tagggcgtgt gccattcctg aaaataatca
    37921 tcgctaagtt ccttaaaact accattggaa atcacccaga caggactccc ctgactgaga
    37981 aaatataaga tatcttccca atgacaacct gttaaatcga taacttttcc tggcagatac
    38041 tgatctgcta atgcttttat aggtttatga tagacaccat agcccatatt ttcaaaacta
    38101 tatatatctc ctacaaagcc tgcattggga tgtccgtaat aaatcttacc gtttttcttt
    38161 tgataaggag taggatcttt ttttatttct tttgccaggg tcattttatc cacttgaacc
    38221 cctgccgatt gaagcatcat agcaagactt gttacttcac agcctctggc cagttcaggc
    38281 aactgtgaaa tcataggtgc ctctaaaata acgctttctt ttaagccaat tgtatttgtc
    38341 catatcatct ttttattatt gtagtaatat atataagact tttgatacct tctggcatga
    38401 gcagaagcct gggcaaaagt ttcaaacgcc ccgagaaact gcttttcccg ataaacagca
    38461 aaaggattat tttggttcca ctgattttgc tttttactga cctctgtgac aaaatttcca
    38521 gtataaatac tgtaaaaaac ataaatgtaa aatagcaata gtacgaattt aagagctttt
    38581 tttaaagata actctttcat catttcaatc ctttttaatt cgttaataca ttatactaat
    38641 aatgccagaa tattattctt ccatctcctg ttcctgcttt tcattctctt gtccgccatc
    38701 ttccgctttc ggcaggtatc gctttaaaat ttttccaata agataatagc taagataggc
    38761 atagataaca acgccaaaaa gaataactgg tggataaatg tatatcgcaa taatcaatat
    38821 ttcaaataat atgatacata gaatggtcgt aaaaaaatgc ttattgccaa tgacaaaagc
    38881 cattttaaaa gccgatgaaa gggttaaata taacctggac aacagtaaaa atatataaag
    38941 gcttataata agtaactgta acaaaacagc agcctgaacg ataaaaatat aacgtcccaa
    39001 ttcaccatac agagcaacat tagacatatt aaatactaaa actgaaataa gtaaaattaa
    39061 gatcagccaa atagctgttg cctgtttaaa attcatttta aaacttttcc aaaaatcctt
    39121 aaaaatatag ccatcttctt tcctgacctt ttttcctacg acataaaaca aagcagtcgt
    39181 tgaggcccca aaggtaacca gcggaatact aaataaaaac cataaaagat ttaaaaatac
    39241 catatcaaac acaaaagttc ctatacggtt aaaagggcca tccaaatcaa aaaatccttt
    39301 tctcattttt gtttcctcca tcattttttc gtgaaatgcc agtccatttt tcaaaactca
    39361 tactgtgctg ctttcattct ttaatttttt cccattaata aattcaacca ttcctttgcc
    39421 atttttcata aaagataaat cgcccatgta tctttcctga gtctggggca aagtatagct
    39481 tattcccttt attactttat ctttaagcaa tctttcaacc atttctgctg ttattgtaag
    39541 accataaatt tccagactgt ttttccaaag ggcaaattta cagccagctt tccattggct
    39601 gcagtaaaag gccttactgt tttccattat actgccttta ttacataaag gacacttccc
    39661 aagccctttt gtttttttag aagacctttt actattcttt ttactctctt tcggaaatga
    39721 aacttttata tcactctttt tagattcatc tacgatttgt ttaacatatt cgataatact
    39781 atccataaaa cgctcaggat ccattttatt tttagctatg gaagataaac ctttttccca
    39841 ttttcctgtt gtttcagggg attttaattc ttcagggaca atttcaatta atttcatccc
    39901 tttttcagta ggatacaaca ctttaccctt tctttcaaca tatccaactt caataagcct
    39961 ttcaatgata gcagcccttg ttgcaggagt tcctaaaccg ctttccttga gttgttcttt
    40021 cagttcttca tcttcgacaa aacgccctgc gttttccata gctgacaaaa gagcagcttc
    40081 tgtataaggc tttggcggcg aagtctgttt tttttccttt tctgccattt ttacaaaaag
    40141 ctcatctcct tttttaacag atggcagttc catttcctct tctttctttt tccccgaatc
    40201 ctctttgtaa aaaatcatcc agcctaactg tcggatcctt tttccctttg aaataaaatg
    40261 ttctttttca atttctgtaa taagctttgt tatggtatat tcataccttg gataaaatac
    40321 ggctaaaaac ctttttacaa ttaaatcata aattttcttt tcatcagagg ttaatttttc
    40381 taatttaggt actgtatccg ttggtataat cgcatgatga tctgttactt tgctatcatc
    40441 aataattctt ttcgttacag gtaagctttc tttttcaaga attggctgga caaactggct
    40501 ataagcagga atattcagtt tatttaatac actgacaaga acggctttca tatccgaact
    40561 taaataccga ctatcggttc tgggataagt aatcattttt ttcttttcgt aaagcacctg
    40621 ggcaactgat aaggttttgc tcgctgaaaa accataatac ttattagcat ctctttgcag
    40681 ttccgttaaa tcatataaaa gaggaggtaa ttgatttttt tcttcttcct gaatttctat
    40741 aaccatcccc tttttatcct ttacaagatt aacaattgca tctacttttg cttcatctgt
    40801 tatttttgtt tcattcttat ctaaatcaat ccatttgcca taaaaattac cataatcaac
    40861 tctgacttcc gagtaatcct ccgaaacaaa attttcaatt tccttttgcc tttgaaccaa
    40921 tatacttagg gtaggtgtct gcaccctgcc aatcgataaa agaacattgt attttatagt
    40981 ataagcccgg cttgcattca tacctaccag ccaatccgct tcagaacggc atttagcaga
    41041 ataatagagc ttgtcatatt cttctcctgg tcttatgctt tcaaaacctt ttttaatagc
    41101 ttcatccgtc atactggaaa tccaaagcct cttaaagggt tttttacagc ctgctgcttc
    41161 ataaatatag cggaaaatca gttccccttc ccggccgctg tctgtcgcac atattaaaga
    41221 atctacctct tttgaatgca ttagtttttt tatgatatta aattgtttta tagtcttttt
    41281 aacaggctta attttcattg gttcaggaat aattggcaaa tcatctaaag accatttttt
    41341 atatttatca tcataatctt ctggcattaa caattcaacc aaatgtccca ttgcccaggt
    41401 tacaatatat ctttctccgg tgatacagcc atcagccttt tccttacaac ctaaaaccct
    41461 tgcaatgtct cttcctacag aaggtttttc tgcgattacc aaaatttttc ccatactctc
    41521 ttcctttcgc aaaacaatta ataatatttt agcatagatt gttttcttcg ttaaccaaac
    41581 atttttttga aaaaatttac atagctttat tgaatgatta cgtactaatt gggaaagcta
    41641 catatataaa ttaaatattt ataagaagtt ttttacttta aataataaat tttggaggga
    41701 atgtatatgg ctgatgaatc aatgaataac agaaaccgca atgatgaatc cttcgggatt
    41761 ggtcatgcca tcataagatt aattgtaaca gctattgtac ttgctataac agctttttta
    41821 accccaggtt tttctgttga tggtatctgg gcattaattg ttgcttccat tgtaattact
    41881 ttactggatt atttaattga aaaaatcact ggcattgatg cttctccttt tggaagaggt
    41941 gttgtaggtt ttattgtatc tgcagcaatc atttatgtta cgcaatattt tgtagcaggt
    42001 tttgatgtta ctttctgggg tgcaatcata ggtgctttag taataggcgt catcaatgcc
    42061 attaccccag gaagagctat gtagcactaa taaattaggg atgcttacag catccctaat
    42121 ttattagtga acggatctcc tgattgattg catcaatatc aacggacggt atttcttcat
    42181 tgtttatatt atatttcatt ttcattgata atattcgata aagactctcg tccaatcttt
    42241 gtccggatat ttctccctcc actactgctt tttttatacg ctcaaggact tttatttcat
    42301 tattatatcc atggcacacc aatacaatat ccgctcctgc attaatggcc tttacggcag
    42361 cttctcccat ttcataatga tctgcaatgg cacccatggt taaatcatca gtaatcacta
    42421 aaccctggaa accaaattga tttcttaaaa tatctccgat aatgacttgt gaaagggatg
    42481 ccggattttg tgaatctact ttatcaaaca gtatatgggc aatcataact gcatcacagc
    42541 cattgttaat agcttctata aaaggaacta attcgaattg tttcatttct tccaaatctt
    42601 tttctatcaa aggtaaattt aaatgagaat ccacctgggt atctccatga ccaggaaagt
    42661 gctttacaac agaaataata ccttctgatt gcataccttc catgactgcc agaccctgtt
    42721 cttttacagt gattacatct gccccaaatg ctcttggtcc aataactgga ttattgggat
    42781 tactgttaat atccagtaca ggtgcaaaat tcatattaaa gccaaaagct tttatttctc
    42841 tggcaataat ttttcctatt ttatatgaat aatctttgtc atttttttcg cctacacttt
    42901 tactatcagg taaattaatc agttctgcag gcatccggct gactaaacca ccttcttcat
    42961 cgacagaaat aaaaagcggt atcttatctt cgttaagtct tttaagagta ttaattaaag
    43021 tcaaaagttg acccgcatca tttacgtttc tcttaaataa aataacgccc cctacataat
    43081 actcttttat cattttttct gtttcttcat ctatagactg tccttcaaaa cctacaataa
    43141 ccaattgtcc tattttttcc tccaggctca tttcttttat cttttctttt ataggatcaa
    43201 tttcttcaga cggattaagt cctgaactgt tatcttcatt gttattctta tcgctgctgt
    43261 tatcttcact attattgtct tcctgtttta cctcttcttt tgatttatct gtactgtcct
    43321 tatcataaga actttcatta ttataataag cagaacatcc aaggcaaaag gctaaaataa
    43381 gcataattag tacaccaatt tttttcaaca ttatatcatt cctcatctat acctaaatat
    43441 caagtccatt atatactatc cattttcata ttaaaatatc acgaaataat ttcttaatta
    43501 tttaatattt taaaggatct cagaaccctg cttttttctc aatgtattat ttccattttt
    43561 ttcctattga acttatggca ttttccagta ttatcaaaaa tcctgcttta tcatttttat
    43621 tcgtaaagcc tttaaataca aaacggatgc aattatcttt aactttttcc ttttttatcc
    43681 tcatcaataa aaaaacacca caaaagcatt tcaaaagttt agacacagct ttcaaaatgc
    43741 tttaatatgg tgtctttagc ttattgcctg tttgaaatca ttttatttat ggcactgata
    43801 agcgctttta tagaagaagt actgatattg gtattaattc ctgcaccaaa gaaagaattt
    43861 ccttcttcat cttcaatatg aatataagat actgccctgg aactggaacc tttatctaaa
    43921 gaatgttcat cataagatac aatcttaaag tctatagaca aatgctcttt tataccatta
    43981 caaaatgcat ccaatggtcc attccctctg ccttctattt cttcttcctg atccgctttt
    44041 ttaatgatta cccgaacaac aatgtcatct tcctggctgt ctatcttata gttttttaag
    44101 ctataaggtt ccactatatt catatattct ttaataaaaa tatcgtgaat catttgtggt
    44161 gtaagttctg tttgatttct gtcagacaca tcagtaataa ttaaactgaa atcttgctgc
    44221 atttttttag gaagtctgaa accaaaatca ttttctaaga cataactaac gccgcctttt
    44281 cctgactgac tgttaatacg gataatcgga tcatagcttc ttcccacatc tttcgggtca
    44341 ataggaagat aaggtacttc ccaatgatct ggtttatctt ttttataggc ttccatgcct
    44401 tttttaatgg catcctgatg agagccggaa aaagctgtat aaaccaattc tcccacataa
    44461 ggatgtcttg gggaaacctc catttttgtg ctttcttcat aaatcttaat aatagcatcc
    44521 gcatcactaa aatccagttc cgggtcaact ccctgagaaa acatattcat acccaaagta
    44581 ataatatcca catttcccgt tctttccccg tttccaaaaa gagttccttc tactctgtct
    44641 gcccctgcta acattgccag ctctgctgct gctacagcag ttcctctgtc attatgggga
    44701 tgaacactaa gaatgatgtt ttctctattt ttaatatgat ctataaaata ctcaacttga
    44761 tccgcataaa cattaggcgt agacatttct accgtagcag gcaaattaat aatggcttta
    44821 ttttctttgg aaggtttcca aacatcaaga actgcttcac aaatttcaat cgcataatcc
    44881 aattctgttc ctgtgaaact ttctggagaa tattcataaa caatagaacc tttgggcatt
    44941 ttttcagcaa attcttttac aatatgtgcc ccttccacag ccaaatcaat aatttcttct
    45001 ttactctttt taaatacaac tcttctttga agctcagagg ttgaattata taaatggaca
    45061 atggctcttt taaccccttc taaagattca aaggtttttc ttataatatg ttcccttgcc
    45121 tgtgtcaata cttgtatggt gacatcatcg ggaattaaat tgtcttcaat gagttttctt
    45181 aaaaaagtaa attccgtttc agaagcagcc ggaaaaccta cttcaatttc cttaaagccc
    45241 atttttacaa gaaagtcaaa aaattttaac ttttgctcta aattcattgg taccttcaaa
    45301 gcctgattcc catcccgaag gtcaacacta caccatatgg gtgcttttgt tatagtcctg
    45361 ttgggccatt ttctgttttt taaatctact gttggataag gtttgtattt tttataattc
    45421 atacccattg atatacctcc ctgttgtttt tcaataataa aaaagtcttt catctctact
    45481 agcagagacg aaagactgaa cttccgcggt accactcttc ttcatccaaa aaggatgcaa
    45541 ctttaaagga tactgcaaca ataaatatat tctcctgata aataatttaa cttttgcaaa
    45601 agctaatccc ttcaaaaata ttctatccta ataaaaaaat ctttcatctc taaagagata
    45661 aaagattatt aaccactctt tatacacagt tttagggata caagcagaga atatacttat
    45721 gatttgctga tatcctgtca tgataacggt tgacttccgt ctcgtcctac tgaacctttc
    45781 agcgagcaac tccgaggcga gttcagtttt tttctgatgc tgtttcacac caaccaacag
    45841 ctctctgaaa tcttccaaaa acctactact cctcttcatt gtttttaatt atttgaattg
    45901 tttaatacta tagcatggaa attgactaaa gtcaataact tattttaatt ttttataaaa
    45961 attaatcaaa ttttgctgtc gataaaagct cagtgatc
//
LOCUS       DAOSUD010000006        45619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_556_length_45619_cov_24.594417, whole genome shotgun sequence.
ACCESSION   DAOSUD010000006 DAOSUD010000000
VERSION     DAOSUD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 45619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45619
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_556_length_45619_cov_24.594417"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1634
                     /locus_tag="PLG49_01695"
     CDS             126..1634
                     /locus_tag="PLG49_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOQ16056.1"
                     /translation="MKIGFDQKKYLEEQSRYILERVNNYDKLYLEFGGKMMYDLHAKR
                     VLPGFDENGKIKLLERLKDKVEVIICVYAGDIERNKIRGDFGITYDMDTLRLIDDLRN
                     HELLVNSVVITRYDEQPAAKMFINKLERRGIKVYKHKAIKGYPTDLDTIVSDEGYGKN
                     PYIETTRPIVVVTGPGPGSGKLATCLSQLYHEYKRGKVAGYSKFETFPVWNIPLKHPL
                     NIAYEAATADLRDVNMIDSFHFDAYSEIAVNYNRDIETFPILKRIIEKITGKDSVFKS
                     PTDMGVNRIGFGIIDDEVIREASKQEIIRRYFKTGCDYKKGYVDKETFEKVKLIMDEL
                     NLKEEDRRVVMPARERAARLKETASKSDICTAVAIEMPDGKILTGKSSKLMDATAAAI
                     LNAVKYLAHISDDILLISPVILEPIKYLKNDILKSANPALNCEEILIALSICAATNPV
                     AQIAMDKLPLLNGCQAHSTAILNRNDEQTLRKLGIDITCDPEYASQDLYYNN"
     gene            complement(1681..2370)
                     /locus_tag="PLG49_01700"
     CDS             complement(1681..2370)
                     /locus_tag="PLG49_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOQ16057.1"
                     /translation="MSIYSLSNFLQEYAQKPDVVEAFQLESDQMLKIDLNGDVWIKTG
                     SMVAYKGVIKFTREKMMEHGLGKAIMKSITGEGATLTKAQGRGELFLADNGKRISVIY
                     LDSNAITVNGNDLLAFSPSIQWDIKFLKNVSSIMAGGLFNMQLSGRGYVAITTHYKPL
                     ILMVTPDKPVVTDPYATVAWSSNLIPELKTDINLGTFIGRSSGETFQMFFRGQGFVVV
                     QPYEEVTFQHS"
     misc_binding    2676..2916
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3058..4242
                     /gene="trpB"
                     /locus_tag="PLG49_01705"
     CDS             3058..4242
                     /gene="trpB"
                     /locus_tag="PLG49_01705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOQ16058.1"
                     /translation="MSKARFGIYGGQFVPETLMNALIELEETYNRYKEDPGFVNELND
                     LLNNYAGRPSLLYYAEKMTKDLGGAKIYLKREDLNHTGSHKLNNVLGQVLLAKKMGKS
                     RVIAETGAGQHGVATATVAALMDMECEIFMGKEDTERQALNVFRMELLGAKVHAVTSG
                     TQTLKDAVNETLREWTRRIADTHYVLGSVVGPHPFPTIVRDFQSVIGKEVKKQILEKE
                     GRLPDVVMACVGGGSNAMGIFYDFIENKEVRLIGCEAAGHGIDTDKHAASMTKGTVGI
                     FHGMKSYFCQDEYGQIAPVYSISAGLDYPGVGPEHSYLKDIKRAEYVPVTDDEAVEAF
                     EYLAKVEGIIPAIESAHAVAYARKLAPTMDKDKIIVICLSGRGDKDVAAIARYKGVNI
                     YE"
     gene            4235..5014
                     /gene="trpA"
                     /locus_tag="PLG49_01710"
     CDS             4235..5014
                     /gene="trpA"
                     /locus_tag="PLG49_01710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOQ16059.1"
                     /translation="MSNIENVFKKGKAFITYITAGDPSMETTIKLVIEMAKAGADVIE
                     LGIPFSDPVAEGPVIEEAHARALSKGITTDQIFDGIKEIRKNSDVPIVIMTYANPIFT
                     YGTERFMKRCQEVDVSAVIVPDIPFEEKEELLPFCQEYNITMISMITPTSKERIRMIA
                     QEAEGFIYCVSSLGVTGVRKSIGNEAEEMIKLVKEVKEIPCAIGFGLSTPQQAAQMAK
                     IADGIIVGSAIVQMIAEYGENSVPYVYEYVKEMKKALMCSF"
     gene            5201..5473
                     /locus_tag="PLG49_01715"
     CDS             5201..5473
                     /locus_tag="PLG49_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16060.1"
                     /translation="MLKIPIEDFIEEIKAEIMSYEELGEKKALEWEKNFISLIHEKDK
                     LIKNIEEENGKKYYCLEDESELFRIVDMYLAAVDSGEEKEYWERWI"
     gene            complement(5470..5883)
                     /locus_tag="PLG49_01720"
     CDS             complement(5470..5883)
                     /locus_tag="PLG49_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16061.1"
                     /translation="MSSTKIIVLQLKEIIYTLLFAIIGFILLMVLIFYFIPGKKESAQ
                     LYESGVYTAPVSLDYAQFNVSVVVEDNQITSVELTDFDDNMALIYPLVEPTMAHLNRE
                     IVKKQSLDVDSPEDASQTANILLDGIKKALSNPQN"
     gene            complement(5951..6136)
                     /locus_tag="PLG49_01725"
     CDS             complement(5951..6136)
                     /locus_tag="PLG49_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16062.1"
                     /translation="MTKYTKLKADANSQTFLYLLEKPAEKKYLIFKKAIYPNKKNIYL
                     FFKDLQVAESHFNQLIS"
     gene            complement(6239..7351)
                     /locus_tag="PLG49_01730"
     CDS             complement(6239..7351)
                     /locus_tag="PLG49_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRK06851 family protein"
                     /protein_id="HOQ16063.1"
                     /translation="MDKKGRIKHVFPGGNTTKGFMSFYNNILFQEEANKIIIIKGGPG
                     VGKSSFMKKISDRFLNLGYNLEYHHCSSDNNSLDAIVIVPLKVAILDGTAPHVVDPIS
                     PGAVDEILNLGDYWNEEGIKIHKEEIIRTNKEIKRVFNKAYNYLKAAYQIYEAWTNTE
                     KLAFNKAKANLLTDKLIKELFEKYPVAKQSGKDRHLFGTAITPGGLTEYLHTIIGESK
                     QIYMIKDSPGASAKDLMNKISEEALIRGFYLECYHSPIDVDKIEDIIIPELSCAITVV
                     NDYHKAKVIPTNIIDLTSCLDSSVTKILKEETDKDKLLFDDLIEKAILIIQKAKKFHD
                     RLESYYVPNMDFDKINDLAGKICKRILEISQADTDK"
     gene            complement(7424..8233)
                     /gene="spo0A"
                     /locus_tag="PLG49_01735"
     CDS             complement(7424..8233)
                     /gene="spo0A"
                     /locus_tag="PLG49_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOQ16064.1"
                     /translation="MVKNTISVLLADDNKEFCEILEDYFKKQSDMEVVGVAKDGIEAY
                     NMIIEKVPDVVILDTIMPRLDGLGVLEKLHSSNIKKIPICIMLSAVNQDKVTQKAMRL
                     GAEYYIAKPFDMETLMLRIRQLTDLAPITPKSLTANNPVENNYGNQIPSPHQLESEVT
                     YILREIGVPANIRGYHYLRHAITMAVNDLDVLNSITKELYPSIAKKFNTTSSRVERAI
                     RHAIEVACNRGSVETMHKLFSYTINNNKGKPTNSEFIALIADRLRLNLKIG"
     gene            8602..9546
                     /locus_tag="PLG49_01740"
     CDS             8602..9546
                     /locus_tag="PLG49_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ16065.1"
                     /translation="MNTSQEFLEKIIENVEKVIVGKRDVIELTIIALICNGHVLIEDV
                     PGVGKTSLVSALAKSLNASFKRIQFTPDVLPSDITGFSIYNQKTGEFEFKPGAIMSQI
                     ILADEINRTSPKTQASLLEVMEENQVTVDGVTYPLPKPFMVLATQNPVEYLGTYPLPE
                     AELDRFFMKFSIGYPTNYEEERILKNFQKENPLEELRTVADYNEILEIQRQVKEVYVD
                     PLIISYIVNIVNETRRHPDLILGASPRGSLSLFKAAQGWAFYKGRNYVIPDDVIKMAI
                     PVLSHRLIIKQEAKLRNVFAEAIIESIIKNKVRVPIIK"
     gene            9730..10788
                     /locus_tag="PLG49_01745"
     CDS             9730..10788
                     /locus_tag="PLG49_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ16066.1"
                     /translation="MLIKYTQKIDKKNLIKGEKIHFNFSIHNEFFLIYPFIRVVFYEM
                     DYIFGRQTKALEFALNPFSHKSFECEWECKYRGDYTVGIDYIEIKDFLGLFSFFYYIR
                     EPKEIKVYPRISMLNYFNLNTSYISESDSIVKSNKEDMSNISDIRKYVYGDTLRKIHW
                     KATAKRNELMVKNYDNTAQIYVNLLLDLKENHFGEEKNMIIEDQVIECIVAVTHYCLR
                     HWIPVKLVYYNEGIKSIEGKNALDFEIFYDFMRKAKFNQNINFSDVISTYLSNEMEKS
                     NWILFTVNLEYDLYDQLYEMSCAGYNIILIYISPEDLVTINKDEEEKIIEGLKDIGVK
                     VIKVNIRDDIKQVLGCSL"
     gene            10785..13076
                     /locus_tag="PLG49_01750"
     CDS             10785..13076
                     /locus_tag="PLG49_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOQ16067.1"
                     /translation="MMRGKFHEFLIDFFLMFLVSFSFAFMGLTDVGFSPAPLFIIRIL
                     VTILLIYSIIFINKKTTVLSLTLSMFSLAIWIIYILKTGRAEIVLIKHYRVLIWLYEY
                     IYNDISVNWTYAKVLSILICSSICLGIYLFTKKKWHYLILTLIGSAYFLYLWYLDYLT
                     VPWTMYLFLFSCLLYFFRHRYYKYGKLIEKENIVAFKWFMLWGMLISTIVFIFSYYYS
                     QKHVIQLQWVRNIVDKYTAEPAYYYESDTFSVMSACFGEGDGRLGGNLTPNEILVLKI
                     SSPKRVYLKGIMKDTYTGHSWLYKGEPYDSFHKTYVGALECREGIKILSDENVDFEKY
                     FEECDLEVEFQNIITKTIFVPENLIKLETDEHVTDRGDGIFLENAKGKGYRYSVKFYY
                     PKKVQEDFIELIRKSKKGLYKERYNQIIQDRESIDEHSKLLLEQLIEQSEDINEQYLN
                     LPESLPERVRDLAYEITKDKETDYDKVKAIEDYFLTNFHYTYNPGDVPLGRDFVDYFL
                     FDSKEGYCTYYATAMTVLVRSIGLPARYVEGYCLTRDTWKGKNEYWVTNMQAHAWTEV
                     YFEGIGWISFEPTPPYRREFDENYGNQNISDFYRNYYQNLLDRTEETNINNEVRTGSE
                     IKINIRIKALYMLLSIFIILIMILVMNLFKVLLKIRKMIHGDGKESAILIYEEYLKIA
                     NLLGYPIKETETPLLYSERVHKIFNDEEITPLFLKARYSPNALNHEEMAVMYNFYKNL
                     IKDTKDKIGKYKFFIWRFILGKI"
     gene            13219..14823
                     /locus_tag="PLG49_01755"
     CDS             13219..14823
                     /locus_tag="PLG49_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOQ16068.1"
                     /translation="MKGVKSFYILMAFLLLFLFSFPDIVHAVDIPYEVRIGLEYKYKN
                     TDRINIYNKEILAGYDTKSGYIPEAIIKSDSGFYMVPSYSYYVSYNQSFDSYEEALYF
                     SQELKSDTFSAFPANFYLAKWYVYVGEFNSYQEAEAAANQLSGKTGRVFIVKEPKSSR
                     IALKNHTGTLVVFEGEEGYPQFYGVDHSPYQGLINLGERQYRGKMEFGRYDGKGITAV
                     NVLPFEEYLYGVVPAEMPASWDIEALKAQAIVARNYAINCMGKHSASGYDLCDGEHCQ
                     VYKGYTTETERSNRAVDETEGELLYYNNELVSTFYFSSSGGYTENSEDVWVTALPFLR
                     AVEDYYELNTYTWTKSFSREEIEVLLKNNGKDIGKILDIQIGSYSTGGRVMELTIIGS
                     NGSVTLTKESIRSFFKKDGASLDSRKFQLVKGGTTNHSYFSVIGGNSKINSNISLNQI
                     YALGADGNITNLSGNLASIVAEGKNGQEHLSLVHSTSAEGDFVFVGRGRGHGVGMSQW
                     GAKGMAEAGFNYRQILAHYFNGAEIR"
     gene            14884..15306
                     /locus_tag="PLG49_01760"
     CDS             14884..15306
                     /locus_tag="PLG49_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16069.1"
                     /translation="MAQGLYDKDNDIEDFDIENERNVSIDKYLPGNLYEPYERGDILL
                     SFCLYTSVEKAGEICTIWGEIQPILEENCKEGVCFQKIKYKYQINIVSYGQKRIILRK
                     KRCQNEYYLLKMTCNQDYLYYKINPVAIIWNILPDLFF"
     gene            15373..15720
                     /locus_tag="PLG49_01765"
     CDS             15373..15720
                     /locus_tag="PLG49_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16070.1"
                     /translation="MDKSNIWFVPKVIGIWLLFVVFPVINQVLAKLMDKEEQITHFIY
                     GTMFCGVLLTLFFAVLLMLTYNREKTLRKYFMRRRRTRIETRDGYYQCLYCGNEMVKQ
                     EDIICSVCGVRFL"
     gene            15790..16815
                     /gene="queA"
                     /locus_tag="PLG49_01770"
     CDS             15790..16815
                     /gene="queA"
                     /locus_tag="PLG49_01770"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOQ16071.1"
                     /translation="MNVHDFYYELPKELIAQDPLEDRSASRLLVLDKNTGQMEHDYFK
                     NILSYLNPGDCLVINDTRVIPARLIGARKDTGAKVEILLLTRKENDRWETLVKPGKKA
                     RPGDVIIFGNGELKAEVEEIIDDGKRIVHLIYQGIFEEVLDRLGEMPLPPYITHQLKD
                     KDRYQTVYAKHRGSAAAPTAGLHFTEELLEKIRNNNIVIANVTLHVGLGTFRPVKVED
                     ITEHKMHSEYFWIEKEEADKINRAKQAGGRIIAVGTTSCRTLESCADESGIIRPQSGW
                     TDIFIYPGYQFKIVDALITNFHLPESTLIMLVSALAGKDNILNAYKTAVEMRYRFFSF
                     GDAMFIR"
     gene            16895..17851
                     /locus_tag="PLG49_01775"
     CDS             16895..17851
                     /locus_tag="PLG49_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ16072.1"
                     /translation="MSNDYNSHELPASNGGNSEILPDNGPVPNSDENQNDDFLPNRPG
                     KKRNWILIAIIALLLLNNFDLTGKLSMFWGKSVNIEENINDCSHLFLKAKNGSIKAKG
                     YNGEKIKLTVRYSKSFFGGNVDFKLRKNGKEARLDYDGKVSGLSIEASIPYDQFSSMT
                     LQSSNGTIETKDLNILNTHIKTSNGSVSVDQWTGEKLTIETSNGRVNLTDVLGDEAEI
                     KTSNGAIDFKAKEFVKDKEYDYSFKTSNGSIHLDLPKDNQIAYKIKAKTSNATIHHNL
                     DHFIGEDDSDSRELSGRTSNYDEAATKVNIEMKTSNASITID"
     gene            complement(17881..18984)
                     /gene="aroC"
                     /locus_tag="PLG49_01780"
     CDS             complement(17881..18984)
                     /gene="aroC"
                     /locus_tag="PLG49_01780"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOQ16073.1"
                     /translation="MAGSIFGKHFQISTWGESHGKALGVVVDGCPAGIELCEEDIQKD
                     LDRRRPGNSPFASPRKEKDAVEILSGVFEGKTTGTPISLIVYNTNQRSYDYSEISKLY
                     RPGHADFTYDQKYGFRDYRGGGRSSGRETTARVAAGAIAKKILQEFNVDITAYTLSIG
                     PVEIDKSKFDKSEIYNNFLAMPDKEAAKAAADYVQNVMDNEDSAGGIIECIVTGLPTG
                     VGEPVFEKLDAMLAKAIFSIGAVKGLEFGSGFEAAKKTGWENNDFFRYDENGKITKLT
                     NHAGGISGGISDGFPIVFRTAIKPTPSIHRMQQTINKNGENADIQVEGRHDPIIVPRA
                     VVVVEAMTALTLVDYLFQRIFSRIDLIKKALLD"
     gene            19172..20314
                     /gene="tgt"
                     /locus_tag="PLG49_01785"
     CDS             19172..20314
                     /gene="tgt"
                     /locus_tag="PLG49_01785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ16074.1"
                     /translation="MYKLLKIEGRAKRGEFHTPHGIIQTPVFMNVATVAAIKGALSSM
                     DLKDVKCQVALSNTYHLHLRPGDQVIHKLGGLHKFMVWDRPILTDSGGFQVFSLTSLR
                     TIKEEGVYFSSHIDGRKIFMGPEESIQIQSNLGSTIAMAFDECPPYPCDKAYMTASVS
                     RTTRWLKRCQAELRRLNQLDTTVNKNQMLFGINQGGVYKDIRIEHAKEISELNLDGYA
                     IGGLAVGESHEQMYYILEETVPYLPQDKPTYLMGVGTPENILESVERGVDFFDCVLPA
                     RNGRHGHVYTNKGKLNLFNAKYELDSSPIDETCTCPTCRNYSKAYIRHLLKAKEMLGM
                     RLCVLHNLYFFNNMMEEIRQALDNNNFAHYKKLKLEGFQEKEEESE"
     gene            20311..20799
                     /gene="yajC"
                     /locus_tag="PLG49_01790"
     CDS             20311..20799
                     /gene="yajC"
                     /locus_tag="PLG49_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOQ16075.1"
                     /translation="MSQLFVLADAAANNSANTTSAGVITTFVYFGVIILIMWFFIIRP
                     QKKREKEILDMQNSVKVGDTVLTNGGIYGKVVDVINDLCIVEFGLNKGVRVPLQKNYI
                     AAIKEPDMTAKKEETEEKIKEVKEEKNKKGKSKKNKTNENKENVKEIQDQIEETEIKE
                     EN"
     gene            complement(20839..21684)
                     /locus_tag="PLG49_01795"
     CDS             complement(20839..21684)
                     /locus_tag="PLG49_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ16076.1"
                     /translation="MKCIDLHVHSSSSDGTLSPRELVLYAKEKNLAAFALTDHDVVDG
                     IEEALAEGQKQGIEIVPGIELAAQYQDREIHILGFFIDYKDPYFLERLQWIQKERKRR
                     NQKMLEKLNDLGFDITLEELKSVAGKDLISRAHFGKLLQEKGYAASITDAFQLYLSPG
                     KAAYVQREVYSPKECIHFIHEAKGLAVLAHPTLYDLSDEEIKDLIKILKAEGLDGIEA
                     IYSLYTNEEEAKLRSWAHHYNLFITGGSDFHGKNKRQIDLGTGRGNLKIPYELLEEMK
                     KRLKK"
     gene            21798..22211
                     /locus_tag="PLG49_01800"
     CDS             21798..22211
                     /locus_tag="PLG49_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOQ16077.1"
                     /translation="MSKNNYKAVQTTKNEKEWRLFEILKGNVIAYMITAIIFIAAALL
                     LTYTGLPETSISLIAILTTIISAFIAGYDAAKGAKARGWFWGMSSGMVYAILLILIGS
                     LVGKSLALTSRTITLLIIAIGSGALGGMIGINVKK"
     gene            22314..22454
                     /gene="scfA"
                     /locus_tag="PLG49_01805"
     CDS             22314..22454
                     /gene="scfA"
                     /locus_tag="PLG49_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOQ16078.1"
                     /translation="MKHVKTLNADTLKKSMSKGGCGECQTSCQSACKTSCTVANQSCE
                     NN"
     gene            22525..23880
                     /gene="scfB"
                     /locus_tag="PLG49_01810"
     CDS             22525..23880
                     /gene="scfB"
                     /locus_tag="PLG49_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOQ16079.1"
                     /translation="MIHKYSIDGMNIVLDIYSGAVHVVDDIVYDLLDFYKEIPNDELI
                     EKFKEKYEIVQIEEAIEEIESLEKEGLLFTEDAYVDYLTEFKNRNPVVKALCLHVAHD
                     CNLKCKYCFAGEGAYKGSRSIMTKEVGIKAIDFLIEASGNRRNLEIDFFGGEPLLNFE
                     LVKEIIEYARSIEKEHNKNFRFTITTNGLLLNDEIQNYINENMHNVVLSIDGRKEIHD
                     KMRYTKNNQGSYDLILPKLKKMAESRKQQNYYVRGTFTRENLDFAQDVIHLADLGFQQ
                     ISVEPVVAPLEMPYALREEDLPVLFNQYEVLAKELIRRNEEGQAFNFFHFMIDLSQGP
                     CVAKRLAGCGSGSEYLAVTPEGDLYPCHQFVGLEDFKIGTVFEGITNHELQKQFQDCN
                     VYAKEECRSCWAKFYCSGGCAANAYQFHKDIHIPYKIGCELEKKRVECALYLKAKLAG
                     L"
     gene            24027..25241
                     /gene="secD"
                     /locus_tag="PLG49_01815"
     CDS             24027..25241
                     /gene="secD"
                     /locus_tag="PLG49_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOQ16080.1"
                     /translation="MLLTLVITGLMVFTAFYGFGVNHYLGADNIRLGLDLAGGVNIVY
                     EADKENPTSAEMAAAKELIQGRLDRGNYTEAEVSIEGEKRLRVDIPGVDDPEKAIEDI
                     GSTALLQFISPEGEVILTGNEVKDARRDVITDNNGISRPVVVVEFNDKGRDAFAEATQ
                     KYINQVIVIALDETIISYPVVEDVITTGTAFISGNFSIEEADELAARIKAGALPFKLN
                     VLQSKSVGAKLGTDALNTSLKAGLIGIILVLLFMLIFYRVSGLAADIALIFYCGTIIV
                     ILSAMEATLTLPGVAGIILSVGMAVDANVIIFERIKEELNSGKSIRAAVDAGFSKAFS
                     AILDGNITTLIAAAILFWLGTGLIKSFAQTLAIGIILSMFTALVVTRIIIKQFVNIGL
                     KNPKLYCANKEA"
     gene            25244..26146
                     /gene="secF"
                     /locus_tag="PLG49_01820"
     CDS             25244..26146
                     /gene="secF"
                     /locus_tag="PLG49_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOQ16081.1"
                     /translation="MDIVGSRKITFTIAIIVILAGLLAMPINAGMGKGILDFDVEFVG
                     GTVMEIHIGQEFDINNDIRPIVIDITGDDSPQITRIDNQGVAIKTKSIDTKTRRELFD
                     ALKEKYQLDENEALLNAEDVSATIGTEMQLKAIKALVVASILMLIYITFRYKDWKFGL
                     SAVIPLLHDVLVVLAVYSILRVPVNNSFIAVILTILGYSINNTIVIFDRIRENKKSVS
                     RQELKELVNQSVNQTMTRSINTTVTTLLTITVLYFLGVPSIKEFALPLIVGLIAGTYS
                     SIFIASPVWYMLSTKKTRTAKSQA"
     gene            complement(26194..26571)
                     /locus_tag="PLG49_01825"
     CDS             complement(26194..26571)
                     /locus_tag="PLG49_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ16082.1"
                     /translation="MSEKFQVGQIVEGTINSIKPFGAFVTLDESTQGLVHISQISQGY
                     LKDIHEAVSVGDKVKVKILSIDEKNNKISLSMKEASETKAADNTVNSTQASPGKDRLE
                     DMLKDFLKVSNEKQAALNKRNNR"
     gene            26729..29149
                     /gene="recJ"
                     /locus_tag="PLG49_01830"
     CDS             26729..29149
                     /gene="recJ"
                     /locus_tag="PLG49_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOQ16083.1"
                     /translation="MIESKYLWINKGQFFDETDELEKTGLLPVTVKVLKNRGINNIDD
                     MVAFLNPDYSGLYNPFLLKDMDKAVNRILTALEKKEKITIYGDYDVDGITSTSILFLF
                     LKENGAMVDYYIPRRLEEGYGLNPEAVHKIKENGTDLMITVDTGIAAVNEVALAKESG
                     IDVIITDHHECQEEIPKADAVIDPKQKDCSYPFKSLAGVGVTFKLIQALAQKLNKAES
                     IWKYMDIVSVGTVADIVPLVDENRIFVKKGFDMIPNTQNTGLEALLKVSGYKGGTVTA
                     GLIGFAVGPRLNASGRIGDAKKGIELFITKDKEKAKIIADELNEENKKRQAIEQEILD
                     EAIQLIEREVDFQKTKVIVLAGEGWHQGIIGIVASKIVDKYYRPTIMLTIENGVAKGS
                     ARSVEGFNLFEALCASSQFLTKFGGHEMAAGLTMAVENIEAFRMYINEYANNKMDEET
                     LIPKLYLDDCIHISEVSLELISQLKKMEPFGMGNPQPVFSIEGEISNLSLLGKEKQHL
                     RIVLEDSNETIDAVGFGFGEYYDKLLPGQKVLAAGCLDINEWNNTVKAQLMIKDLKST
                     IEDEIKFHYYFSLYKLFLSLNDECTKIDFQSFEELSADDILNEDSLILIQDLELLLEL
                     LRYFGNAKIMFNNRSKICYTNKYSLKNKIEIIVNPIMETIDFSAYKRIIISDALWVNK
                     TFSIAKDKKERFFKLEKKRFSSHKSFSPYSLERKDFEVLYRFLALLSRLKQNSITMDA
                     LIKECNKSFKTNPFKILLSLDIFMELGLIQYEYKNDKLSFEIVSGKKISLEDSKILQK
                     LRKLNEKT"
     gene            29174..29686
                     /locus_tag="PLG49_01835"
     CDS             29174..29686
                     /locus_tag="PLG49_01835"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOQ16084.1"
                     /translation="MDFKSLIRTIPDFPEEGVLFRDITPVLQNREGLKAAIDQMQACI
                     KDIEFDLIVGPESRGFIFGVPLAYNLNKGFIPVRKKGKLPYKTLQKEYALEYGTAIVE
                     MHVDAIQPGQKIVIVDDLLATGGTSKAIVDLVKEAGGEVVKLVYLIELEALKGREALK
                     GYNVESIIKY"
     gene            29771..30463
                     /locus_tag="PLG49_01840"
     CDS             29771..30463
                     /locus_tag="PLG49_01840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ16085.1"
                     /translation="MDIPILYEDGHIIVALKPPKIPSQADKSGDKDMLSYLKEYLKEQ
                     YPMAQNPYIGLVHRLDRPVGGLMVFAKSKWANAKLSEQIQNKQFNKEYLAVVTGKPEK
                     VQGELVDYLLKNQKLNLSKVVSKETKGAKEAILEYECLDSVEFENEGWLSLLKIVLKT
                     GRHHQIRVQLSHAGIPVWGDNKYNPAFVKRREWTQIALWAYSLSFYHPKTNQLCLYIE
                     KPEQYPFNQFNM"
     gene            30592..32829
                     /locus_tag="PLG49_01845"
     CDS             30592..32829
                     /locus_tag="PLG49_01845"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOQ16086.1"
                     /translation="MSDQIIYEKLISLLKSYHPSNDFSLVEKAYRFAKEAHEGQHRKS
                     GEPYVIHPLEVAVILAELELDIESITAGILHDILEDTDHTFEELAGLFGEEVARLVDG
                     VTKLGKINFSSNNKELQKEEIQAENYRKMFLAMAQDIRVILIKLADRLHNMRTLRFMP
                     PEKQKEKANETLHIYAPLAHRLGISKIKVELDDLALRYLEPEVYYDLAEKIAKRRIER
                     IEYVERIVKEVKTKLDEAGIKATVEGRPKHFFSIYKKMIGKNVTLDQIYDLFAIRAIV
                     ETVKDCYGVLGIVHEMYKPIPGRFKDYIAMPKPNMYQSLHTSLIGPEGEPFEVQIRTR
                     EMHKVAEYGIAAHWRYKEGKKGTKVTDKSQDKTEEKLAWLRQILEWQREMSDNKEFLD
                     ALKMDLDIFSDQVYAFTPKGDVISLPYGSTPIDFAYMIHSAVGNKMVGAKVNGKIVTF
                     DYQIKNGDRVEILTSQNSRGPSRDWLKNVKSSQARSKINQWFKKQYKQENINRGKELL
                     EKDAKKRGLNINDLLKAEWIEKVLRKYGFQDWDAVCAAVGHGGIKEGQVIHRLYEEYR
                     KENAKNLIKEETITIQDDSKKPVKKSKSGVVIEGVGDVAVRFSKCCNPVPGDEIIGFI
                     TRGRGVSIHRTDCINILNISEEEKDRLIEAEWLVAEKDFKNITYQTEIKVIGEDRMGL
                     IVDISRVLTEEKIPVKALNARTTKNNESIINITIEISGIQQLETIIKKIRSIPGINDI
                     IRTTT"
     gene            32839..33474
                     /locus_tag="PLG49_01850"
     CDS             32839..33474
                     /locus_tag="PLG49_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ16087.1"
                     /translation="MILKVLSVGLIGTNCYICGDEESKKGVIIDPGADAEFIISELKK
                     ENLDIEYILITHGHFDHIGALEKVKEFTGAKVLIHEEGVAYLEDAYLNLSKAFFDKEF
                     VKSADGTLRDGDMIEVGNLSFRVIHTPGHTSDGVNYYEEKNGILFSGDNLFRTSIGRT
                     DFPGGDMHLLVSSIKEKLLSLPEDVVVYPGHGEPTSIGYEKQNNIYLSENS"
     gene            33485..34897
                     /gene="hemZ"
                     /locus_tag="PLG49_01855"
     CDS             33485..34897
                     /gene="hemZ"
                     /locus_tag="PLG49_01855"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOQ16088.1"
                     /translation="MTPYELVGHTHSYQIENILRLYEKYIEDISIKSILKENICRAEI
                     YKNNHLLIYKEEKDIKDPRLQKQKLASVLYQALVEMTGKEMPWGFLTGIRPTKIVHEM
                     MEENLTDEEIKKRLLEFYFIREDKIQLMMEVAKKEKEILDKNKDNELSIYIGIPFCPT
                     RCLYCSFTSYAIDKREKQVEDYLDALEKEILSSRKYLKDKFIRSLYIGGGTPTSLNAA
                     QLERLLIQINKSFFMDEIEEFTVEAGRPDTITKEKLNLLKKYHVNRISINPQTMDNET
                     LVRIGRKHNKEDIIKAFYMARELGFDNINMDLILGLPGEDRKNLSYTMSQIQKLSPDS
                     ITVHTMAIKRASRLKETLDDYSLIDENEIEKMIEMSYESAIQMGMQPYYLYRQKNMLG
                     NFENVGYAKVGKESVYNIQIMEEKESILALGAGAITKLVDKENNKIERIANVKNVDEY
                     IKRIDEMILRKEAGMDFFEK"
     gene            34992..36251
                     /gene="hisS"
                     /locus_tag="PLG49_01860"
     CDS             34992..36251
                     /gene="hisS"
                     /locus_tag="PLG49_01860"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOQ16089.1"
                     /translation="MLTKAPRGTKDLFGDEMNAWLEMEKIIRDLCKDFGYGEIRTPVF
                     EHTELFQRGVGETTDIVQKEMYTFQDKKGRSITLKPEGTAGAVRAYLEQKLYAGIQPT
                     RLYYITPAFRYEKPQAGRMRQFHQFGIELLGSDSPTADAEVITVAYELLKRVGISKVK
                     LHINSLGGPECRKKYNEKLKEYLKDNLDHLCETCRDRFERNPLRILDCKTESCKKIVS
                     DAPTVLDVLGDECRAHFEKVKALLDEMGIPYIIDPGIVRGLDYYTKTVFEFISTDIGA
                     QGTICGGGRYNNLVEELGGQPTPAVGFAVGLERLLLVIEAENGKKTYPVTKDIFIGSI
                     GDAALLKAHSLIYQLRQAGVTAEGDHFNKSVKAQMKYADKIGAKYSMIIGDDEINNGK
                     AILKNMETKEEREVHLDELVTTMKSIL"
     gene            36272..38059
                     /gene="aspS"
                     /locus_tag="PLG49_01865"
     CDS             36272..38059
                     /gene="aspS"
                     /locus_tag="PLG49_01865"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ16090.1"
                     /translation="MGESMLGLKRSHYCTQVNESLIGQEVVVMGWVLKRRNLGSLIFI
                     DLRDRTGILQIVFDEKDLNANDFSKVESLRSEFVIAVKGLVRNRESINPNLATGMIEV
                     VAKELRILSEAETPPFAIEEGSSVKEDLRLKYRYLDLRRPDLQRNLMLRHKVAQSVRK
                     FLDSEGFLEIETPMLARSTPEGARDYLVPSRIHKGSFYALPQSPQTFKQLLMVSGFDK
                     YFQIVKCFRDEDLRADRQPEFTQIDMELSFVDVDDVIDVNERLLKTVFKEALGIDIPL
                     PIPRLTYQEAMDRFGSDKPDIRFGLELVDISDVVQDCGFKVFEEAIANGGSVRGINAK
                     NCANFSRKQIDALVEVGKTYGAKGIAWLAIGEDGTIKSTFTKFLSEEICQEIIKAMDG
                     QKGDLLLFCADKNSVVYDALGNLRLEIAKRLNLINKNEYSFVWVTEFPLLEWDEEENR
                     YVAKHHPFTMPMDEDIELLNTDPGKVRAKAYDICLNGVELGGGSIRIYRKDIQEKMFN
                     ALGFTKEAAYENFGFLLNAFKYGVPPHGGLAYGFDRMIMLMTQADSIRDVIAFPKVKD
                     ASCPMTDAPGKVTEQQLDELGLKLDENNE"
     gene            38215..38856
                     /locus_tag="PLG49_01870"
     CDS             38215..38856
                     /locus_tag="PLG49_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16091.1"
                     /translation="MSNRKNQILVGLIIVAMGLFTLFGQWISIPFIRKNNLFGLFIAT
                     TLLLLYCTKKKTWALVAGMFIGFFSVLGIFPNVNFNTATFIAPMVFILPGFIFIILYY
                     SKYNIGFLIPGSILMWFGIFIFLVTSGLMRGMMIPVAWFGSLGAAFLSMYLLGRHKIG
                     KWPLIPGGILLGMAFLLFAGASLGFIFRLGPKLIPVTLIIIGLFIVIKSRKSN"
     gene            38948..39700
                     /locus_tag="PLG49_01875"
     CDS             38948..39700
                     /locus_tag="PLG49_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOQ16092.1"
                     /translation="MLHAFSRTELLLGKEAMEKLYKSKVAIFGIGGVGSFVVEGLVRS
                     GIGKFILVDDDSVCLTNLNRQLHATRKTVGRAKVEVMKERILEINPRAEVKVHQTFFL
                     PETSEEIIDDDCDYIVDAIDTVTGKIELVLQAKRKNIPVISCLGAGNKLDPTKFEVDD
                     IYNTSICPLAKVMRRELRKRGIESLKVVYSKEVPIKPKEEEVMTCREGCICSNKERTC
                     LEKRQIPGSVSFVPSVAGLIIAGEVVKDLISR"
     gene            39726..40166
                     /locus_tag="PLG49_01880"
     CDS             39726..40166
                     /locus_tag="PLG49_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOQ16093.1"
                     /translation="MKYRKRGEVGQVIDVKQNIAIISMTRNEACQQCGACSHGHRSEE
                     MILEAKNLCNAKVGDKVVIDLSSSDFLKATFIMYGLPLILLFTGFIIGYYGSYKLGYT
                     SLQEPMGVLFGFLFMTFSYFWIRANEEKWEGQNYRPVAVKIVEE"
     gene            complement(40221..41729)
                     /locus_tag="PLG49_01885"
     CDS             complement(40221..41729)
                     /locus_tag="PLG49_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16094.1"
                     /translation="MTKERINYHIIKQSTGNLWAFFYDDSEGICYMTCDNDHWSKKQR
                     LYSSGSKNFSVYLDKQDNIYLYCQDSSGNIILYQNTAGKWESQVLLYSKNGFIDSLSF
                     DAIIENDNFYLFYNLKDPNSNRHALVEQKATSGKQWKTPALIDYIYPLDKKPFTVYKD
                     NQDNIFISYQKYSEYYQLGYKKYSASSKNWSDFLNFDKSHFSFTDESILASQDELFNL
                     YIKKEAHYSTLYFRSKTDNQWKEAKKLFEKADISSCSLFMIDDHLWAAWICQDKLYSC
                     YSTDRGNSFSEAAIHLIEEPALILKAHYQSNFPEEEKRLLCNEIYILTNPQIKFLILP
                     EVFPAINGTSIQINHTFIPATEKVDNYLNEIKIHLNQLYEEIYICKKQLREKENQINQ
                     LKYSLKVKNKDISKTAYDFSKINEQQKKELTALKERIKKLEEELTKKNEKIKQLEEKN
                     TALKKGMDILKKELETRKAPLIITTDSGENKKEKGNKPSIFKKIFSFEEENS"
     gene            complement(41878..43236)
                     /locus_tag="PLG49_01890"
     CDS             complement(41878..43236)
                     /locus_tag="PLG49_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16095.1"
                     /translation="MACFLKNPDGTLIMVNYSPRKGLYYQIFMDDHTSRPNLLLANGT
                     YEYSAFLDENSNLHVIAKNDKSQIIHFQLLSNKIEKEIILDDYANEFKISDIFALSFK
                     NQVHLLYYADNPSTKNTELIYQLLKKPYCAPVPLGEIPNREINFDCIRQKDNVLLSMI
                     SKEQDDYTINIKALNPQSNKWVDFYQPVKSKFPVKYCKLILDQSGICHLLYIQEQYGQ
                     YQLIYKNNKEGWSNSHPLYSCGYFIKPVLFIYQDALWINWNENGTFKSLLSVDMGDSF
                     SETIHNSIQDSGASIHYYENKLSDSPLICNQLYGISHYPPQFVLLYNLDMDGIHPHLK
                     VNNELKLYLTHIKKQLNISVSKRKLEEENHRLKDELEKMTNIQMDISHQYNELAELAR
                     KIQEEGKKWRNLYKEAKSEIEFHKKRIKQLEKISEAKISTENQKQVELPKEDEKKEKE
                     EI"
     gene            43375..44319
                     /locus_tag="PLG49_01895"
     CDS             43375..44319
                     /locus_tag="PLG49_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16096.1"
                     /translation="MACCNNNQVRGRFFQRPTAGECPVCKPDEIINPAEDACVCPPLG
                     EPKLLTVAAPVVFDECGINLCRVVDNLDKIFCNVDFERDDVQGIELKVIDIDFNFCND
                     DGSNVETLVRRPNCVRVTLTEIDVTFAAKVIDRNCRVIDEECFTIRYLPGNEEDPYFD
                     NDANPVSISVDIYAPYGVSYNLNGDCTPVISFLGFVENGSEGNNALRQGLVAQALAKV
                     VNLDIDSLSAAIGLTIYLKSIYFVQYKIPHKGLCVPPKCIPVDIEAENACLDFVEGDL
                     LEQSIQPLELCCKPKSIKHGFSAVEDPTPAEECGKKRS"
     gene            44510..45325
                     /locus_tag="PLG49_01900"
     CDS             44510..45325
                     /locus_tag="PLG49_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16097.1"
                     /translation="MGRRDDECIIALKVYDQCRQQDCLTPEMLDRVFSLESGECVVGD
                     QIIAAGRGSIINLPVGTVDIAVKDFKISKIETDRKQDVFNPDYYFVHVKYFFKFKLSF
                     LDGDGCPIAITCDGVERDAMEAGTAFKKTVKLYGSEGSDIAISSNLFAPESHVLEAAP
                     YVLVEAKAMLLDAQIDTNDGLTCECGEEAIGEINLTIGLFTIIKLYRLVNLLVESSGF
                     CEPEECDEVASVDPCSIFENMDFPFDIFNPPQKNDARCGRRGSGSDRDDYEED"
     gene            45403..>45619
                     /locus_tag="PLG49_01905"
     CDS             45403..>45619
                     /locus_tag="PLG49_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16098.1"
                     /translation="MSKCREHRRDCIDDKKCIISNAINGLQKTLNSCCCCVIRKLEEL
                     INEQRSAFCIANSVCEAAEEGDAEIAVI"
BASE COUNT        15441 a         6458 c         9038 g        14682 t
ORIGIN      
        1 gtggacatat ttttttaatt tccagatagt aatatcttta ctttatgaga aacctatgta
       61 ataattaagg tttgagttta taattattta tgcttgaaaa atatactaaa aagtggggaa
      121 agaacatgaa aataggattt gatcaaaaga agtatttaga agaacaatca agatatattt
      181 tagaaagggt taataattat gataagcttt atctggaatt tggcggcaaa atgatgtatg
      241 atttgcatgc caagagagta ttgccgggtt ttgatgaaaa cggaaaaatt aaacttttag
      301 aaagactaaa ggataaagta gaggttatta tttgtgtata tgctggagac atcgaaagaa
      361 ataaaatccg aggagatttc ggtatcactt acgatatgga taccttgcgc ttaattgatg
      421 atttaaggaa tcatgaattg cttgtcaata gcgttgttat taccagatat gacgagcagc
      481 cagcagcgaa aatgtttatt aacaaattag aacgaagagg gattaaagtt tataaacata
      541 aggctataaa aggttatccg acagatttag ataccattgt aagtgatgaa ggctatggta
      601 aaaatcctta tattgaaaca acaagaccta tcgttgttgt aacaggtccc ggaccaggca
      661 gtggtaaact ggctacatgt cttagccaat tgtatcatga atataaaaga ggaaaagtgg
      721 caggttattc taaatttgag acttttccag tatggaatat tcctttaaag catcctctga
      781 atattgctta tgaagctgca acagctgatc tcagggatgt caatatgata gactcctttc
      841 attttgatgc ctattcagag atagctgtta attacaacag ggatatagaa actttcccta
      901 ttttaaaaag gattattgaa aagataacag gaaaggattc ggtatttaaa tctccgacgg
      961 atatgggtgt taacagaata ggatttggaa ttattgatga tgaagtgata agagaagctt
     1021 ctaaacaaga aatcattaga agatatttta aaacaggttg tgattataaa aaaggttatg
     1081 ttgataagga gacatttgaa aaagttaaac tcataatgga tgagcttaat ctgaaagaag
     1141 aagacagaag ggtagttatg ccagccagag agcgagctgc caggttaaaa gaaactgcaa
     1201 gtaaaagcga tatttgtaca gcagtagcta tagagatgcc agatggaaaa attttaaccg
     1261 gaaaaagttc gaaattaatg gatgcgactg cggcggctat attaaatgct gttaagtatt
     1321 tagcacatat atcggatgat atcctgttaa tttcacctgt cattttggaa cctatcaaat
     1381 acttaaaaaa cgatatactt aagagtgcaa atcctgcttt aaattgtgaa gaaattttga
     1441 ttgcacttag tatatgtgct gctacaaacc cagtggccca gatagcaatg gataaattac
     1501 cattattaaa tggctgtcag gcacattcta cagcaatact caacagaaat gatgaacaaa
     1561 ctttaagaaa attgggcatt gatattactt gtgatccaga atatgcttct caggatttat
     1621 attataataa ttaataataa cccggaattc atgaatgaat tccgggttat tttcataaac
     1681 ctaagaatgc tgaaaagtaa cttcttcata aggctgtact acaacaaatc cctgacctct
     1741 aaagaacatt tgaaatgttt cgccgctgct tcgaccgatg aatgttccca gattaatatc
     1801 agtttttaat tctggtataa ggtttgaaga ccaggccaca gtagcatagg gatcggttac
     1861 aacaggttta tcaggagtaa ccattaaaat aaggggttta tagtgggttg taatagctac
     1921 ataacccctg ccacttaatt gcatattaaa cagaccacca gccataatac tggaaacatt
     1981 tttaaggaat ttaatatccc attggattga agggctaaag gctaataaat catttccatt
     2041 aactgtaatt gcattggaat ccaggtaaat aacagagatt cttttaccat tatctgctaa
     2101 gaataattct cctcttccct gggctttagt cagggttgct ccttcacctg taatagattt
     2161 cattatagct ttaccaagcc cgtgttccat cattttttcc cttgtaaatt taatgacacc
     2221 cttgtaggca accatagagc cagtttttat ccagacatct ccgttcaagt ctattttgag
     2281 catttgatcg ctttctaatt gaaaagcttc aacaacatct ggtttttgtg catattcctg
     2341 aagaaaattt gataaagaat aaatactcat tgaagacctc cttgtagttt tattaccagg
     2401 cataagcctt agttatgata taaagtgcat cgatggtcta tgttgatttg aaggatcttt
     2461 ctcataagct tttccaaaaa caaaagctta gtctatggga agcattagaa ttccttgaaa
     2521 attatttaaa ctattataaa ttatataatg aaatataaat ttttaaaagt gtaattattt
     2581 taaaataata gctctttata ctagaataat ttattgacaa aataaaatta aaatgctatt
     2641 atcaagctta aatataagga atttttgatc aaaaaattaa accgatgagc aagagtagta
     2701 gaaataatta ctgtattcag ggagtcgctg tttggtgaga atgcgattac aagtgttatt
     2761 tcgaatggac ttgcgaggga agcccgaaat taatagtagg cgcttacggg aaccctgacc
     2821 gttatcaaaa gggtgtatat gaaagtatac aaatagagtg ggttgtttca accaatttgg
     2881 gtggcaccgc agaagcgata agcttttgtc ccttacggga taaaagcttt ttttattaat
     2941 gaaacaaggg atggttccga aacacggtga cggttccttg tttcaaaaga ttaataaaac
     3001 aaggacccgt caccgtgttt catgaaaaat aaaataatca attaaaggag aatggaaatg
     3061 tctaaagcaa gatttggaat ttacggaggt caatttgttc ctgaaacctt aatgaatgct
     3121 ttaattgaac tggaagaaac ctacaatcgc tataaagagg atcctggttt tgttaatgaa
     3181 cttaatgatt tgttaaataa ttatgctggt cgtccttcac tgctttatta tgcggaaaaa
     3241 atgactaaag atttgggcgg ggcaaaaatt tatttaaaaa gagaagatct caatcataca
     3301 ggttctcata aattaaacaa tgtcttaggg caagtgcttt tggctaaaaa gatgggcaaa
     3361 agcagagtta ttgcagagac aggagcaggt caacatggtg ttgcaacagc aacggttgct
     3421 gcccttatgg atatggaatg tgaaatcttt atggggaaag aagataccga gcgacaggct
     3481 ttaaatgtct ttagaatgga acttttaggg gctaaggtcc atgccgtaac ttccggaacc
     3541 cagacattaa aagatgctgt taatgaaact ttaagggaat ggaccagaag aattgccgat
     3601 acccattatg ttctgggctc agttgtagga ccccatcctt ttcctacaat tgtaagggat
     3661 ttccaaagtg ttatcggaaa agaagttaaa aaacagattt tagaaaaaga aggaagacta
     3721 ccagatgtgg taatggcttg tgttggcgga ggcagtaatg ccatggggat tttttatgac
     3781 tttattgaaa acaaagaagt ccgtttgatt ggctgcgaag cagcaggtca tggcattgat
     3841 acggataaac atgcagcttc tatgacaaaa ggaaccgtag gaatattcca tggaatgaaa
     3901 tcttactttt gtcaggatga gtatggtcaa attgctccgg tatattccat atctgctggc
     3961 ctggattatc cgggggtagg accagagcat tcttacctga aagatattaa aagagcagag
     4021 tatgtaccag tgactgatga cgaagcagtg gaggcttttg aataccttgc aaaagtcgaa
     4081 gggattattc ctgccattga gagtgcccat gcggttgctt atgcacgaaa attggctccc
     4141 actatggata aggataaaat cattgttatt tgtctttcag gcagaggaga caaagacgtg
     4201 gcagcaatag cacgttataa gggggttaat atttatgagt aatatagaaa atgtatttaa
     4261 aaaaggaaag gcttttatta cttatattac tgctggagat ccttctatgg aaacaaccat
     4321 taaactggtt attgaaatgg ctaaggcagg agccgatgtt attgaacttg gaattccttt
     4381 ttccgatccg gttgcagaag gacccgttat cgaagaagct catgcccggg cgctttcaaa
     4441 aggtatcaca accgaccaga tatttgatgg cataaaagag atcagaaaga attctgatgt
     4501 cccaattgtt atcatgacct atgctaatcc gatttttacc tatggaacgg aaagatttat
     4561 gaaaagatgt caggaagttg acgtttcagc agttattgtt ccagatattc cttttgaaga
     4621 aaaagaagag cttttacctt tttgtcagga gtacaacata acaatgattt ctatgattac
     4681 gcccacttct aaggaaagaa tacgtatgat tgcccaagag gcggaaggtt ttatttactg
     4741 cgtttcttct ttaggagtaa cgggtgtgag aaaatccata ggcaacgaag cagaagaaat
     4801 gattaagctg gttaaggaag taaaagagat tccttgtgct attggctttg ggttatctac
     4861 tccccagcaa gccgctcaaa tggcaaaaat agcagatggg attattgttg gcagtgccat
     4921 tgtacagatg attgcagaat atggagaaaa ctcagttcct tatgtgtatg aatatgttaa
     4981 agaaatgaaa aaagcattaa tgtgttcatt ttaaaaaata agcgagaaaa aaatgccggt
     5041 atttgttaaa taccaggcat tttttatttt acagaaatta aaaatgtttt ataatgattt
     5101 aaaaggcttt tgaaatgttt atttggaata aaataaagaa acattttcaa ataataaaga
     5161 taaaataaca ataatataat tttccaggag gaatgttttt atgctaaaaa ttccaataga
     5221 agattttatt gaagaaatta aagcagaaat aatgtcttat gaagaacttg gagaaaagaa
     5281 agcacttgaa tgggaaaaaa attttatatc tttaattcat gaaaaagata aattgattaa
     5341 aaacattgaa gaagaaaatg ggaaaaagta ttattgttta gaagacgaat cagaattatt
     5401 cagaatcgtt gacatgtatc tcgctgctgt agattctggt gaagaaaagg aatactggga
     5461 aagatggatt tagttctgag gattacttaa tgcttttttt attccgtcaa gaagaatatt
     5521 agcggtttgt gaagcatctt caggagaatc tacatcaagg ctttgttttt taacaatttc
     5581 tctattcagg tgagccatag taggttcaac aagaggatat attaatgcca tattgtcatc
     5641 aaaatccgtc agttctactg atgtaatctg attgtcttct acaacaacag aaacattaaa
     5701 ttgagcataa tccagtgaaa caggagcggt atagactcct gattcataaa gttgtgcgct
     5761 ttcttttttg ccaggaataa aataaaatat gagaaccatc aataatataa aaccaataat
     5821 tgcaaataat aatgtataaa tgatttcttt taattgtagc acgattattt tggtggaaga
     5881 cataaaaagc ctccttcata tcttcgttac aatttatata tatgaaagaa ggtataaaat
     5941 atgccttcaa ctatgaaatc aattgattaa agtggctttc tgccacttga agatccttaa
     6001 aaaaaagata aatatttttc ttgtttggat aaatggcttt tttaaaaatc agatattttt
     6061 tttctgctgg tttttccagc aggtaaagaa atgtttgtga atttgcatct gctttaagtt
     6121 tagtatactt agtcatttaa ataccccttt ctatttaatt ccttgaagag tttaccatat
     6181 attaacatta ttttcaatag aaagaattat cgaaaaaagt agggattttc tccctacttt
     6241 acttgtctgt atcggcctga gaaatttcta aaattctttt gcagattttt cctgctaaat
     6301 cattgatttt atcaaaatcc atatttggca cgtaataact ttctaatcta tcatggaatt
     6361 ttttagcttt ttgaataatt aagatggcct tttcaataag atcatcaaaa agtaatttgt
     6421 ctttatctgt ttcttctttt aagattttcg taacagagct atccaaacaa ctggtcaggt
     6481 caatgatatt tgtaggaatc acttttgctt tatgatagtc gttaactacc gttatggcac
     6541 aactaagctc cggaataata atatcttcaa ttttatcaac gtcaatcgga gaatgatagc
     6601 attccaggta aaaaccccgt atgagagctt cttcagagat tttgttcatt aaatctttag
     6661 cagaagcacc tggtgaatcc ttaatcatat atatttgttt actttctcca ataatggtat
     6721 gcaggtattc ggtcagaccg cctggggtta tagctgttcc aaacagatgt ctgtcttttc
     6781 ctgattgttt tgccacagga tatttttcaa acagttcctt aatgagttta tcagtaagaa
     6841 gatttgcttt ggctttgtta aaggctagtt tttccgtgtt agtccaggct tcatagattt
     6901 gataggcagc ttttaaataa ttataggctt tgttaaatac tctttttatt tctttgtttg
     6961 ttcttataat ttcttcttta tgaattttga tgccttcttc attccagtaa tcacccagat
     7021 ttaggatttc atcgactgct ccagggctaa tgggatctac aacatgggga gcagtaccgt
     7081 ctaaaatagc cacttttaga ggaacaataa cgatcgcatc taaagagtta ttatctgatg
     7141 aacagtgatg atattcaagg ttataaccta aattaagaaa tctatcgctg atttttttca
     7201 taaatgaaga cttccctacc cctggtccgc ctttaataat aatgatttta ttggcttctt
     7261 cctgaaatag tatattatta taaaaagaca taaaaccctt agttgtattg cctccaggaa
     7321 aaacatgctt aatgcgtcct tttttatcca tgaactccct cctttaataa taaaaattgg
     7381 ggtattaaat aaatgtccct aaggaattcc ttagggatat ttgttatcca atttttaaat
     7441 ttaaccttaa gcggtcagca ataagagcga taaattcgct gttggttggt ttccctttgt
     7501 tattatttat ggtataacta aaaagtttat gcatggtttc aacactgccc cggttacagg
     7561 caacttctat ggcatggcgg atggctcttt ctacacggct tgaagtggtg ttaaattttt
     7621 tagcgataga aggatagagt tcttttgtaa tactgttaag gacgtccaga tcatttacgg
     7681 ccatggtaat cgcatgacgt aaataatgat agcctcttat attggcaggg acaccaattt
     7741 ctctaagtat gtaggtgact tcactttcta attggtgagg ggaagggatc tgatttccat
     7801 aattgttttc aacagggttg tttgctgtca aactttttgg agttattgga gcaaggtctg
     7861 taagttgtct tattctcaac attaatgtct ccatatcaaa gggttttgca atataatatt
     7921 ctgcaccaag acgcattgct ttttgagtca ctttatcttg attaactgct gataacataa
     7981 tgcagattgg gatctttttt atatttgatg aatggagttt ttctaataca cctaaaccat
     8041 ctaagcgggg cataatcgta tctaatatca caacatcagg aactttttct ataatcatat
     8101 tataggcttc tattccgtct tttgctaccc caaccacctc catatcactt tgttttttga
     8161 aataatcttc aagaatctca caaaattctt tgttatcatc tgccagtaat acactaattg
     8221 tatttttcac aatgaatctc ctttctctta agtcattttc tttttctaaa tacaatttat
     8281 atacaaaaat accataaaag aatattacaa tatggaatta taccataaat gtaaaaaaat
     8341 tcaaatagat aaaattaata tcctatatcc tttcttatga atttgtcatt ttgtatatga
     8401 aaaatataca tcttttagta aaattataca aatcatttat agaattgagt tagtttgtta
     8461 aattttaaat aagataataa gattgttttt atcaaaaata tttagaatat gtttcctaag
     8521 ataaaatctt ttgatataat ttatttatgt aaaagagttg aatattaaag tcttttcaaa
     8581 aaaccattgg gggggaaaaa aatgaatacg agccaggaat ttttagaaaa aatcattgaa
     8641 aatgtcgaaa aagttattgt tgggaaaaga gatgttatag aattaacgat tattgcatta
     8701 atatgtaatg gccatgtttt aatagaagat gtaccaggtg taggaaaaac aagtctggtt
     8761 tctgcactgg caaaaagcct gaatgcttct tttaaaagaa ttcagtttac acctgatgta
     8821 ttgccttcag atataacagg tttttcaata tataatcaga agacaggaga atttgagttt
     8881 aagccaggtg ctattatgag tcaaattatt ctggctgatg aaattaaccg gacatcacct
     8941 aaaactcagg ccagtttatt agaagttatg gaagaaaacc aggtcactgt tgatggggta
     9001 acttatccat taccaaaacc ttttatggta ttagccaccc aaaaccctgt agaatattta
     9061 ggaacctatc ctttaccaga ggcagaatta gaccgttttt ttatgaaatt tagtattggt
     9121 tatcctacca attatgaaga agagagaata cttaaaaact tccagaaaga aaaccctctt
     9181 gaagaattac gaacagttgc agactacaat gaaattttag aaattcaaag gcaggttaaa
     9241 gaagtatatg tggatccgtt aattatttct tatattgtga atatcgtcaa tgaaaccagg
     9301 agacatccgg atttaatttt gggtgccagc cccaggggtt ctttgtcttt gtttaaggca
     9361 gctcagggat gggcttttta caagggacga aactatgtta taccggacga tgtgattaaa
     9421 atggcgattc cggttttgtc ccaccgctta attattaaac aggaagccaa attaagaaat
     9481 gtttttgctg aggcaatcat tgaatccatt attaaaaata aggtcagagt gccaataatt
     9541 aaataagatt tgcactcttg aatgagcgga ggttgtgata atgtggtaaa gacgaagccc
     9601 ctatatttta ttatttttcc tgtcatttta gtatttattt actttttcca gggggagatt
     9661 ccttccatat acttttatat ccctcttttc ttggtaatta tttcttttat ttatggtgga
     9721 atgctttggt tgttgattaa atatactcaa aaaattgata aaaagaattt aataaaagga
     9781 gaaaaaattc attttaattt tagtattcat aatgaatttt ttctgatata tccttttata
     9841 cgagttgttt tttatgaaat ggattatatt tttggcaggc agacaaaagc tttagaattt
     9901 gcactgaatc cctttagtca taaaagtttt gaatgtgagt gggaatgtaa atatagagga
     9961 gattatacag taggtatcga ttatattgaa ataaaggatt tcttaggttt gttctctttt
    10021 ttttactata ttagagagcc aaaagaaata aaggtctatc ccagaattag catgcttaat
    10081 tattttaatt taaatacttc gtatatttca gagtcagaca gcattgtaaa aagcaataaa
    10141 gaagacatgt caaatatatc cgatatcaga aagtatgttt atggtgatac tctaagaaaa
    10201 attcactgga aggcaacagc aaaaagaaat gaattgatgg ttaaaaatta tgataatact
    10261 gcccagattt acgtcaattt attattagat ttaaaagaaa atcattttgg tgaagaaaaa
    10321 aatatgatta ttgaagacca ggtaattgaa tgtattgtag cggttaccca ttattgtctc
    10381 cgacattgga ttcctgttaa attagtttat tataatgaag ggataaaaag cattgaagga
    10441 aaaaatgctt tagattttga aatattttat gatttcatgc gaaaagcgaa atttaatcaa
    10501 aatattaact tttctgacgt gattagtaca tatttaagta atgaaatgga aaaaagcaat
    10561 tggattttat ttacggttaa tttagaatac gatttatatg atcagttata tgagatgtct
    10621 tgtgcgggct ataatattat actgatttat atatcgccgg aagatcttgt tacaatcaat
    10681 aaagatgaag aggaaaaaat tattgagggt ttaaaagata ttggtgttaa agttattaaa
    10741 gtaaacatca gggatgatat aaaacaggtt ttggggtgta gcttatgatg agggggaaat
    10801 ttcatgaatt tttaattgac ttttttttga tgtttcttgt aagtttttct tttgctttta
    10861 tggggctgac tgatgtgggg tttagccctg cccccctgtt tattatcaga atacttgtaa
    10921 caatactttt gatatattct ataatattta tcaataaaaa aacgactgtt ttaagtttaa
    10981 ctttatcaat gttttctcta gcgatatgga ttatctatat tttaaaaaca ggcagagcag
    11041 aaattgtatt aattaaacat tatagagttc tgatatggtt atatgagtat atttataatg
    11101 atatttcggt caattggact tatgcgaagg tattatccat attaatctgt agttcgattt
    11161 gtctcggaat atatctgttt acaaaaaaga aatggcatta tcttatcctt acattgattg
    11221 gaagtgcata ttttttatat ttatggtatc ttgactatct gactgttccc tggaccatgt
    11281 atttattttt attctcttgt ttgctatatt tcttccggca cagatattat aaatatggta
    11341 aattaataga aaaagagaat atcgttgcat tcaaatggtt tatgttatgg ggaatgctta
    11401 taagcacaat agtatttatt ttttcttatt attattccca aaagcatgtt attcaattgc
    11461 aatgggtgag aaatattgtt gataagtata cagcagagcc ggcttactat tatgaatcag
    11521 atactttttc agtcatgtct gcttgttttg gtgaaggaga cggcaggctt ggaggcaatt
    11581 taacacctaa tgaaatttta gttttaaaaa tatcgtctcc caaaagggtc tatttaaaag
    11641 gaattatgaa agatacatat acaggtcata gttggctata taaaggagag ccatacgatt
    11701 ctttccataa gacatatgtg ggtgctttgg aatgcaggga aggaattaag attttgtcag
    11761 atgagaatgt tgattttgaa aagtatttcg aagaatgtga tttagaagta gagtttcaaa
    11821 atattattac aaagacaatt tttgttccgg aaaatttaat taaactggaa acagatgaac
    11881 atgttacaga caggggagac ggaatatttt tagaaaatgc aaaaggaaag ggttatcgtt
    11941 attctgtcaa attttattat ccaaaaaaag ttcaggaaga ttttattgag ttgataagaa
    12001 agagtaaaaa aggcttatac aaggaaagat ataatcaaat tattcaggat agagaaagca
    12061 tcgatgagca tagtaagctt ttgttggagc agcttattga acaatctgaa gatatcaatg
    12121 aacaatattt aaatcttcct gaaagtcttc ccgaaagagt cagggatttg gcttatgaga
    12181 ttacaaagga caaagagacg gattatgata aggtaaaggc tattgaagat tattttttga
    12241 caaactttca ttatacttat aatccaggag atgttccttt gggaagagat tttgtagatt
    12301 attttttgtt tgattcaaaa gaaggctatt gtacctacta tgctacggcg atgacagtac
    12361 ttgtaagaag cataggtctt cctgcaagat atgtagaggg ttattgtctg accagagata
    12421 cctggaaagg aaagaatgaa tattgggtaa ccaatatgca ggcacatgca tggacagaag
    12481 tatattttga agggataggc tggatttctt ttgagccaac gcctccttac agaagagagt
    12541 ttgacgaaaa ctatggaaac caaaacatat cggattttta caggaattat tatcaaaatc
    12601 ttttagatag gacagaggaa acgaacataa ataatgaagt tagaactggt tccgaaatta
    12661 aaataaatat aagaataaaa gcgttataca tgcttctatc tatttttatc attttaataa
    12721 tgattcttgt catgaatctc tttaaagtcc ttttaaaaat tagaaaaatg attcatggag
    12781 atgggaaaga gtccgctata ttgatttatg aggaatactt gaaaattgca aatttattag
    12841 gatatccaat taaagaaaca gaaacgcctt tgttgtattc tgaaagggtt cacaaaatat
    12901 ttaacgatga agaaataaca cctttattct taaaagcaag gtatagccca aatgctttaa
    12961 accatgaaga aatggcagtc atgtataatt tctataagaa tttaataaaa gatacaaagg
    13021 ataaaattgg aaaatacaaa ttttttattt ggagatttat attagggaaa atatgacaaa
    13081 aaggggcata aggctccttt ttttgtctct tttcataaga ggatatagat ttttgtaata
    13141 ttttcctata taatgatgat gtataaattc tgttttcctg gcagaagttt ttcaaatcat
    13201 aataagaggg ggatttatat gaaaggggtc aaaagctttt acatattgat ggctttttta
    13261 ctgcttttct tattttcatt tcctgatatc gttcatgctg tggatatacc ttatgaagta
    13321 agaattggtc tggaatataa atataaaaat acagatagaa ttaatatata taataaagaa
    13381 atcctggcag gatatgatac gaagagtggt tatatacctg aagctattat taaatcagat
    13441 agtggttttt acatggttcc atcttattcc tattatgttt cctataatca atcttttgac
    13501 agttatgaag aagcacttta tttttctcag gaattaaaat cagacacttt ttctgctttc
    13561 ccggctaatt tttatttggc aaagtggtat gtttatgtag gggaatttaa ctcatatcag
    13621 gaagcggaag ctgctgcaaa tcaattaagt ggaaaaacag ggagggtttt tattgttaaa
    13681 gagcctaaat caagccgtat agcattaaaa aaccatacag gtactttggt tgtatttgaa
    13741 ggagaagagg gatatcctca attttatggt gttgatcatt caccatatca aggtcttata
    13801 aatttaggtg aaaggcaata tcggggaaaa atggagtttg gaagatatga cggaaagggg
    13861 attacagctg ttaatgtcct gccttttgaa gaatatttat atggagttgt tcctgctgaa
    13921 atgcctgcat catgggatat cgaagcgttg aaggctcagg ctattgtagc cagaaattat
    13981 gcaataaatt gcatgggaaa gcatagtgct tcaggatatg atttatgtga tggggagcat
    14041 tgtcaggttt ataaaggcta caccacagaa acagaaagaa gcaatcgggc tgttgatgag
    14101 acggaaggag aacttctgta ttacaataat gaattggtaa gtacttttta tttttccagc
    14161 agcggaggtt acactgaaaa ctcagaagat gtttgggtta cagctcttcc ttttttaagg
    14221 gcagtagaag attattacga actgaatact tatacttgga caaaaagttt tagcagagaa
    14281 gaaatagaag tgttgctaaa aaataatggc aaagatattg gaaaaatatt agatattcaa
    14341 attggatctt attcaactgg tggcagggta atggaattga ccattattgg gagcaatggt
    14401 tcagtgacat taacaaaaga gagtatccgt agttttttca aaaaagatgg tgcttcatta
    14461 gatagcagga agtttcaact ggttaaggga ggaaccacta atcattctta tttttcagtt
    14521 attggtggaa attccaaaat aaattcaaat atatctttaa atcagattta tgctttggga
    14581 gccgacggca atatcacaaa tttatcaggt aatcttgctt ctatagtagc agaaggaaaa
    14641 aatgggcagg aacatttatc gcttgttcat tcaacttctg ctgagggaga ttttgttttt
    14701 gtaggacgag gcaggggtca tggtgtcgga atgagtcagt ggggtgctaa aggcatggca
    14761 gaagcaggct ttaattacag gcaaatactg gcccattatt ttaacggtgc agaaataaga
    14821 tgaaataaga tattaatact ctttttgtag caggttattt accacaataa agcagtaaaa
    14881 gctgtggcac aagggttata tgacaaggat aatgacatag aagactttga tattgaaaat
    14941 gaacggaatg tttcaattga taaatattta cccggaaatc tttatgagcc ttacgaaaga
    15001 ggggatatat tactgagttt ttgcttatac acttcagtag aaaaggctgg agaaatatgt
    15061 acaatatggg gtgaaatcca gccaatattg gaagaaaact gtaaagaagg ggtctgtttt
    15121 caaaaaataa agtacaaata ccagataaat attgttagtt atggtcaaaa gcggattatt
    15181 ttgagaaaaa aaagatgtca aaatgaatat tatctgttaa aaatgacttg taatcaggat
    15241 tatttatatt ataaaataaa tccggtagcc attatatgga atattctacc ggacttattt
    15301 ttttgatttg tttaattgat aatagtggca aattttagta tcatataaaa aatatatttt
    15361 ggggggagtt gaatggataa aagtaatatc tggtttgtcc caaaagtgat aggaatatgg
    15421 cttttatttg tagtattccc tgtgattaat caagtattgg ccaaattaat ggataaagaa
    15481 gaacaaatta cacattttat atatggaacg atgttttgtg gtgtattact tacccttttt
    15541 tttgctgtac tgctgatgct tacttataac cgggaaaaaa cgcttaggaa atattttatg
    15601 cgaagaagaa gaacccggat agaaaccagg gatggttatt atcaatgtct ttactgcggc
    15661 aatgaaatgg ttaaacagga agatattata tgctcagtat gtggtgtaag atttttataa
    15721 tttgttgtta tgaaagccat ttgacgaaaa ctttatagca tagattttag tagaatgggg
    15781 tattttacga tgaatgtaca tgatttttat tatgaattac caaaggaatt aattgctcag
    15841 gatcctttag aagacaggtc tgcctcgcgg cttttagttt tggataaaaa cacaggtcag
    15901 atggagcatg attattttaa aaatatttta tcttatttaa atcccgggga ttgtcttgtc
    15961 atcaatgaca ccagggtgat tccggcaaga ttaattggtg ccagaaaaga tacaggggca
    16021 aaagttgaaa ttttactgct gacaagaaaa gaaaatgaca ggtgggaaac attggttaaa
    16081 ccaggaaaga aagcccgccc gggggatgtg attatttttg gaaatggtga attgaaagcg
    16141 gaagttgaag aaatcattga tgatggtaag cgtattgttc atttaattta tcaaggtatt
    16201 tttgaagaag tactggatcg tttaggggaa atgcctctac ctccctatat aacccatcag
    16261 ctaaaagaca aggacaggta tcagactgta tatgcaaaac acaggggttc ggcagcagca
    16321 ccaacggcag ggctccattt tacagaagaa ttattggaaa agataagaaa taacaatatt
    16381 gttattgcca atgtcacttt gcatgtcggg ttagggactt tcagacctgt aaaagttgaa
    16441 gatattacag agcataaaat gcattccgaa tacttttgga ttgaaaagga agaggcagat
    16501 aaaattaacc gggcaaagca agcaggcgga cggattattg cagttggaac gaccagttgc
    16561 agaaccctgg aatcttgtgc agatgaatct ggcattatac gccctcaaag tggttggacg
    16621 gacattttta tttatcctgg ctatcaattt aaaattgtag atgctttaat tacaaatttt
    16681 catttgcctg agtcaacatt aattatgctg gtttctgctt tagccggcaa agacaatata
    16741 ttaaatgcat ataaaacggc agtagaaatg cgctacaggt tttttagctt tggagatgca
    16801 atgtttatcc gatagcatct ttaatgattc attaaacttt ttatagtatc cttcgtatct
    16861 attgatatga atgggatgat gaggaggata ttatatgagc aatgattata acagtcatga
    16921 attaccagcc agtaacggtg gaaacagtga aatcctgcca gataatggac cagttccaaa
    16981 ttctgatgaa aatcaaaacg atgatttctt gcctaatcgg ccagggaaaa aaagaaattg
    17041 gatactgata gcaatcattg ctttgttatt attaaataat tttgatttga cggggaaatt
    17101 atctatgttt tggggaaaaa gtgttaatat tgaagaaaat attaatgatt gcagtcattt
    17161 gtttttgaaa gcaaaaaacg gctccattaa ggcaaaagga tataatggtg aaaaaattaa
    17221 gctgacagtt cgttatagca aaagtttttt tggaggcaat gttgatttta aattaagaaa
    17281 aaatggtaaa gaagccagat tagattatga tggaaaagtt tctggtttat ctattgaagc
    17341 gtctattcct tatgatcagt tttcttcaat gactttacag tccagtaatg gaacaataga
    17401 aacaaaggat ttaaatattt taaacactca tattaaaact tctaatggca gtgtgtctgt
    17461 tgaccagtgg acgggagaaa aattaacgat tgagacttct aatggaagag ttaatttaac
    17521 agatgtttta ggagatgaag ccgagataaa aacctccaat ggggcgattg attttaaagc
    17581 aaaagaattt gtaaaagata aggaatatga ttactcattt aaaacttcta acggcagtat
    17641 tcatctggat ttgccaaagg ataatcaaat tgcttacaaa ataaaagcta aaaccagtaa
    17701 tgcaacgatt catcataatt tagaccattt tatcggggaa gacgattcag atagcaggga
    17761 attaagcgga agaaccagta attatgatga agccgctaca aaagtaaata tagaaatgaa
    17821 aacgagtaat gcttccatta ctatagatta aacaacctgc agggaatcct gcaggttatt
    17881 ttaatccagt aaggcttttt ttatcagatc aattctggaa aaaattcttt ggaacagata
    17941 atcaaccaga gtaagggcag tcattgcttc aacgacaacg accgctcttg gtacaataat
    18001 ggggtcgtgt cgcccttcca cctgaatatc cgcgttttct ccatttttat taatcgtttg
    18061 ttgcattcta tgaatggaag gtgtgggttt tattgcagtc ctgaacacaa tagggaaacc
    18121 atcactgata ccgccggaaa ttccacccgc atgattggtt aattttgtga tttttccatt
    18181 ctcatcatat ctaaaaaaat cgttgttctc ccagcctgtc ttcttagcgg cttcaaaacc
    18241 gctgccaaat tctaaacctt tgacggcacc aatagaaaat atagcttttg caagcatggc
    18301 atccaatttc tcaaagacag gctctccgac accagttggc aggcctgtta caatacattc
    18361 aatgatacca ccggcggaat cttcattgtc catgacattt tggacgtaat ctgctgctgc
    18421 ttttgctgct tctttatcag gcatggccaa aaaattattg taaatttcac ttttatcaaa
    18481 tttgctttta tcaatttcaa caggtccaat gcttaaagtg taggctgtta tgtccacatt
    18541 aaattcttgc aggatttttt ttgcaatagc accagcagca actcttgcag tggtttcacg
    18601 gcctgaggaa cggcctcccc cgcgataatc cctgaatcca tatttttggt cataagtaaa
    18661 gtcagcatgt ccgggccggt ataatttcga tatttctgaa tagtcgtagg atctttggtt
    18721 ggtattatat acgattaaag aaataggggt tccagttgtt tttccttcaa aaacgcctga
    18781 taaaatttct actgcatctt tttcttttct gggagaagca aaaggtgaat tgccaggtct
    18841 tcgtctatct aaatcttttt gaatgtcttc ttcacaaagt tcaatgccgg cagggcaacc
    18901 gtcaacgaca actcccaggg ctttaccatg agattcgccc caagtagaga tttgaaaatg
    18961 tttaccgaag atagaacctg ccatgaaatg tctcctttcg ttgagaattt tttaaatttt
    19021 atcattgatt gacttctttt tcaagcctat aaaaattttt atacaatcat aatttcccct
    19081 tgaaaaaaaa gttcattcac tgtacactga tatatagctg catgctggta tgcttaattt
    19141 gttaattgct ttggaaagag gaagaaaaga tatgtataag ctattaaaga ttgaaggaag
    19201 agctaaaaga ggagaatttc atacacccca tggcatcatt caaacgcccg tttttatgaa
    19261 tgtagctacc gtcgctgcga ttaaaggggc tctgtcgagc atggatttaa aagatgttaa
    19321 atgccaggtg gctctttcca atacatatca tcttcacctt agaccaggcg atcaagtgat
    19381 tcataaatta ggtggcttac ataagtttat ggtttgggac agacctatac tgacggattc
    19441 aggcggtttt caggtctttt ccctgacttc cttaagaacg attaaagaag aaggtgttta
    19501 cttttcttct catattgatg gcagaaagat ttttatggga cctgaagaaa gcattcaaat
    19561 tcaatccaat ttgggttcta cgattgcgat ggcttttgat gaatgccctc cttatccttg
    19621 tgataaggct tatatgaccg cttctgtttc ccggacaacg agatggctga aaagatgcca
    19681 ggcagaactt agaagactta accaactgga tacaacggtt aataaaaatc agatgttgtt
    19741 tggtattaat cagggagggg tctataaaga catcaggatt gaacatgcca aagaaatttc
    19801 tgagcttaat ttggatggtt atgccattgg aggtttggcg gtaggagaaa gccatgaaca
    19861 gatgtattat attttagagg aaaccgttcc atatttgccg caagataaac caacctatct
    19921 gatgggtgta ggaacaccag aaaatatttt agaatccgtg gaaagaggcg tggacttttt
    19981 tgattgtgtt ttaccagcaa gaaatggccg gcatggacat gtttatacaa acaagggcaa
    20041 actgaatctt tttaatgcta aatatgaatt agattcaagt ccaatcgatg aaacttgtac
    20101 ttgtccgact tgcagaaatt acagtaaggc ttatataagg catttactca aagcaaaaga
    20161 aatgttgggg atgcgtcttt gtgttctcca taatttatat tttttcaaca acatgatgga
    20221 agaaatacgg caggcattag acaataataa ttttgctcat tataaaaaat taaaattaga
    20281 aggctttcaa gaaaaagagg aggaatcaga atgagtcaac tattcgtttt agcagatgcg
    20341 gctgctaaca attcggcaaa tacaacatcc gcaggtgtca taacgacatt tgtatatttt
    20401 ggagttatta tactgattat gtggtttttt attataagac ctcaaaagaa aagagaaaaa
    20461 gaaatcttag acatgcaaaa tagtgttaaa gtgggcgaca ccgttttaac caatggtggt
    20521 atctatggaa aagtagttga tgtaatcaat gatttgtgta ttgtagaatt tggtcttaac
    20581 aaaggcgtca gagtaccact gcaaaagaat tatattgcag caattaaaga acctgatatg
    20641 actgccaaaa aagaagagac agaggagaag ataaaggaag taaaagaaga aaagaataaa
    20701 aagggaaaat caaaaaagaa taaaacaaat gagaataaag aaaatgtaaa agaaatacaa
    20761 gatcaaatag aagaaactga aataaaagaa gaaaattaat tttaaaataa acacatccag
    20821 ctttatacat ggatgtgttt atttttttaa cctttttttc atttcttcca gcagttcgta
    20881 tggaatttta agatttcccc ttcccgtacc caaatcaatt tgtcttttgt tttttccatg
    20941 gaagtcactg ccacctgtta taaaaagatt ataatgatgt gcccatgagc gtaatttggc
    21001 ttcttcttca tttgtataaa gagagtaaat ggcttctata ccatccagtc cttctgcttt
    21061 taaaatttta attaaatctt taatttcttc atcagataaa tcatacaggg taggatgggc
    21121 taagacagct aaaccttttg cttcatggat aaagtgaata cattcctttg gagaatagac
    21181 ttctctttga acataagcag ctttccccgg ggacagatac aactggaaag catctgttat
    21241 actggctgca tagccttttt cctgcagaag ttttccaaaa tgggctctgg aaattaagtc
    21301 tttgccggca acgcttttta attcctccag ggttatatca aagcccaggt catttaattt
    21361 ttcaagcatt ttttgatttc ttctttttcg ttccttttga atccattgaa gacgttccaa
    21421 aaagtaagga tctttataat ctataaaaaa acccagaatg tgaatttcac ggtcctggta
    21481 ttgtgcagcc aattcgattc caggaacaat ttcaattccc tgtttttggc cttcagcaag
    21541 ggcttcttct ataccatcaa ccacatcgtg gtctgttaaa gcaaaagcag ccagattttt
    21601 ttcttttgcg tataaaacca attcacgtgg agataaggtt ccatccgatg aactagagtg
    21661 cacatgaagg tcgatgcatt tcatggaata accacctttc aaaaatctat ctgcctttat
    21721 cataaatgat taatcataat cgtacaatag taaagagata aattatagct gtgctataag
    21781 ttgaaatgga ggcaaaaatg tcaaaaaata attataaagc ggtgcagacg acaaaaaatg
    21841 aaaaggaatg gagactgttt gaaatcctga aaggcaatgt aattgcttat atgattacgg
    21901 ctatcatttt tattgcggct gctttattat tgacttatac gggattgcct gaaactagta
    21961 tttcccttat tgcaattctt acaaccatta tttctgcttt tatagccggt tatgatgcag
    22021 caaaaggagc aaaagcccgc ggctggtttt ggggaatgag ttcaggtatg gtatatgcaa
    22081 ttttattaat tcttattggg agtttagttg gaaaaagcct tgcgctgaca agcaggacaa
    22141 ttactttatt gattattgcc ataggaagtg gggctttagg aggtatgata gggattaacg
    22201 tgaaaaaata atagcttttc aagaataata aaatgtgtta taataggtaa gctgatgggg
    22261 ccaatgtaca taataacttc cgtcttatct attatttgaa ggaggcgtat ttgatgaaac
    22321 atgtaaagac tttaaatgcg gatacattaa agaaaagcat gtcaaaaggt ggctgcgggg
    22381 aatgccagac atcttgtcaa tctgcttgta aaacttcttg tacagtagcc aaccaatctt
    22441 gtgaaaacaa ttaattttgg cagtggcaat tatgctactg ctttttcaat gaaatgattt
    22501 aattaactat atggagtgaa agaaatgatt cataaatatt ctatagatgg aatgaatatt
    22561 gttttagata tttatagcgg tgcggttcat gtagtcgatg atattgtcta tgatttgctg
    22621 gatttttata aagaaatacc taatgatgaa cttattgaaa agtttaaaga aaaatatgaa
    22681 atcgttcaaa ttgaagaagc catagaagaa atcgaaagtt tagaaaagga aggtttatta
    22741 tttacagaag atgcttatgt tgattatttg actgaattta aaaacaggaa tcctgttgta
    22801 aaagctttat gccttcacgt agcccatgac tgtaatttga aatgtaagta ttgttttgca
    22861 ggagaaggag cttataaggg cagccgttct ataatgacta aagaagttgg tataaaagcc
    22921 atagattttc ttattgaagc ttctggtaat cgccgtaatt tggaaattga tttttttggt
    22981 ggagagccct tgttaaattt tgagctggtt aaagaaatca tagaatatgc aagaagcatt
    23041 gagaaggaac ataataaaaa ttttcgtttc accataacaa ccaatggact tttattaaac
    23101 gatgaaatac aaaattatat taatgaaaat atgcataatg tagtattaag cattgacggc
    23161 aggaaagaaa tacatgataa aatgcgctat actaaaaata atcagggcag ttatgatctt
    23221 atccttccta aacttaagaa gatggctgaa agcaggaagc agcaaaacta ttatgtaaga
    23281 ggaactttta caagagaaaa cttagatttt gcccaggatg tgattcattt agcagattta
    23341 ggttttcaac agatttctgt agaacccgtg gtagcccctt tagaaatgcc ttacgcttta
    23401 agggaagaag atttgccagt tttatttaat caatatgaag tattagctaa agaactgatt
    23461 cgaagaaatg aggaaggaca agcctttaat ttctttcatt ttatgattga cctttctcag
    23521 ggaccttgtg tagcaaaaag attagcaggt tgtggttcgg gctctgaata tctggcagtt
    23581 acaccagaag gggatttata tccttgtcat caattcgttg gtcttgaaga ttttaagata
    23641 gggacagtct ttgaaggaat aactaatcat gaacttcaaa aacagttcca ggactgcaat
    23701 gtttatgcaa aagaagaatg ccgctcttgt tgggcaaaat tttactgcag tgggggctgt
    23761 gcggcgaatg catatcaatt tcataaagat atccatatac cttataaaat tggttgtgag
    23821 ttagaaaaga aaagggtcga atgtgccctt tatctgaaag ctaaattagc aggtttgtaa
    23881 aataaagcta ttaattttct ttaatggtaa aatgtaataa aaagatgtaa aaatattgac
    23941 cttttttttg ataaagcata taattaactt tgtgtattta caatgaaggg agataaggac
    24001 aaatgaaggt taaaagcggg ttgatattgt tattaacact agttattacc ggcttgatgg
    24061 tatttacagc cttttatgga tttggtgtaa accattattt aggagctgac aatataagat
    24121 taggtttgga cctggcaggt ggggttaata ttgtatatga agcagataaa gaaaatccta
    24181 ccagtgcgga aatggcagca gcaaaagaat taattcaagg tcgtttggac agaggcaatt
    24241 atacagaagc agaagtatct atagaagggg aaaaacgtct gcgtgttgat attccaggtg
    24301 ttgatgaccc tgaaaaagcc attgaagata ttggttcaac agcacttctt caatttatta
    24361 gtccagaagg agaagttatt ctaacaggaa acgaagttaa agatgccaga agagacgtta
    24421 ttacggataa taatggtatt tcaagaccgg tggttgtagt agagtttaat gataaaggcc
    24481 gggatgcttt tgctgaagca acacaaaaat acattaatca agttattgtg attgccttag
    24541 atgagacgat tattagttat cctgttgttg aagacgttat tactacaggt actgcattta
    24601 tcagcgggaa tttttcaatt gaagaagctg atgaattggc tgccagaata aaggctggtg
    24661 cactcccttt taaattaaat gttcttcaaa gcaaaagtgt tggcgcgaaa ttgggtaccg
    24721 atgcactcaa caccagttta aaagcaggct taattggtat tattctggtt ttactgttta
    24781 tgttgatctt ctatagagta tctggcttag ctgcagatat tgcattaatc ttttattgtg
    24841 gtacaatcat tgttatttta agtgctatgg aagcaacttt aacccttcca ggtgtagcag
    24901 gaatcattct ttctgttggt atggcagtgg atgctaatgt catcatcttt gagagaataa
    24961 aagaagaatt aaatagtggc aaaagtatcc gggctgctgt tgatgcggga tttagtaagg
    25021 ctttctcagc aattttagat ggcaatatta ccactttaat tgctgcagca atcttatttt
    25081 ggttaggtac cggacttatt aagagttttg cacaaacctt agctatcggt attattcttt
    25141 caatgtttac tgctttagtt gttacacgaa tcataataaa gcagtttgta aacattggcc
    25201 ttaaaaatcc aaaactttac tgtgcaaata aggaggcctg aagatggata tagtaggaag
    25261 cagaaaaata acctttacca ttgcaattat tgttatttta gctggacttt tagcgatgcc
    25321 cattaatgca gggatgggca aaggaatttt agattttgat gttgaatttg ttggcggaac
    25381 agtaatggag attcatattg gacaagaatt tgatattaat aatgatataa gaccaattgt
    25441 tatagatatt accggagatg acagtccaca aataacaaga attgataatc agggagttgc
    25501 cattaaaaca aaatccatcg ataccaagac aagacgggaa ttatttgatg cccttaaaga
    25561 aaagtatcaa ttggatgaaa atgaagcttt attaaatgca gaggatgtat ccgcgactat
    25621 tggtacagaa atgcaactta aagcaataaa agcattagtt gttgcttcaa ttttaatgtt
    25681 gatatatatt acttttagat ataaagactg gaagtttggt cttagtgcag ttattccttt
    25741 gctgcatgac gtactggttg ttcttgcagt gtattctatt ttacgggttc cagtcaataa
    25801 ttcatttatt gctgttatat taactatttt aggttattcg attaataata ccattgttat
    25861 ctttgacaga atcagagaaa acaagaaaag cgtaagcaga caggaattaa aagaattagt
    25921 aaatcaaagt gttaatcaaa ccatgacacg ttctattaat acgaccgtaa caaccttatt
    25981 gactattacc gttttatatt tccttggtgt accttctata aaagaatttg ccctgcctct
    26041 tattgtagga ttgattgcag gtacttattc ttcaatcttt attgcaagcc cagtatggta
    26101 tatgttaagc actaaaaaaa caagaacggc taaaagccag gcataaaatg aagatataaa
    26161 aaaccttcgg cccgatttga gccgaaggtt tttttagcgg ttatttcttt tatttaaagc
    26221 cgcttgtttc tcgtttgaaa cttttagaaa atcttttaac atgtcttcta aacgatcttt
    26281 tcctggagaa gcttgggtac tattaacagt attatcagca gcttttgttt cagaggcttc
    26341 tttcatagaa agagatattt tattgttttt ttcatctatg gacaagattt taactttaac
    26401 tttatctcct actgaaacgg cctcatgaat atcttttaaa taaccttgac tgatttgaga
    26461 aatgtgaact aaaccttgag tggattcatc taaagtaacg aatgctccaa aaggtttaat
    26521 actgttgatg gtaccttcta ctatctgtcc aacttgaaat ttttctgaca tattaacact
    26581 cctatataat attgctatat tattatagca cattgctaca gttttgggta cataaaaaac
    26641 gaattatgac atttaagata gacttttttt tatccctgac atataatatt tttatgtgaa
    26701 atatgaggat atatagaatg gtgataggat gatagaatca aaatatttat ggatcaataa
    26761 gggacaattt tttgatgaaa cagatgaatt agagaagaca ggtttgttgc cagtaaccgt
    26821 aaaggtttta aaaaatcgag ggataaataa tattgatgat atggttgctt ttttaaatcc
    26881 agattattca ggtctgtaca atcctttttt attaaaagac atggataagg cagtgaacag
    26941 gattttaact gcattggaaa agaaggagaa aataacaatt tacggggact atgatgtaga
    27001 tggcattaca agtacatcta ttcttttttt gtttttaaaa gaaaatggag cgatggttga
    27061 ttattatata cccagacgtc ttgaagaggg ttatggttta aatcctgaag ccgttcataa
    27121 aataaaagaa aatggcacgg atttaatgat taccgttgat acaggaattg ctgcggtcaa
    27181 tgaagtagct ctggcaaaag aatcaggtat agatgttatt ataacagatc atcacgaatg
    27241 ccaggaagaa attccaaaag cagatgcggt cattgatcct aagcaaaagg attgtagtta
    27301 tccttttaaa tctttggcag gggttggggt gacatttaaa ttaattcagg ctttagccca
    27361 aaaactaaat aaggcagaaa gcatttggaa atacatggat atcgtttcag ttggtacggt
    27421 ggcagacatt gtacccctgg ttgatgaaaa tcgaattttt gtaaaaaaag gttttgatat
    27481 gattccaaat acccaaaata caggcctaga ggctctttta aaagtatcgg gctataaggg
    27541 gggaacggtg acggccggct taattggttt tgctgtaggg cccaggctta atgcctcagg
    27601 aagaattggt gatgcgaaaa aaggaataga attatttatt acaaaggata aagaaaaggc
    27661 aaaaataatt gcagatgaat taaatgaaga aaataaaaag cggcaggcta ttgagcaaga
    27721 aatattagat gaggccatcc agcttattga aagagaagtc gattttcaaa aaacaaaggt
    27781 aattgtcctt gctggggaag gctggcatca gggaattata ggaatcgttg cttcaaaaat
    27841 tgttgataaa tattatcggc caacgattat gttgacgata gaaaatggtg ttgctaaagg
    27901 ttctgccaga agcgttgaag gctttaatct ttttgaagcc ctttgtgcaa gcagccagtt
    27961 tttgacaaaa tttggtggtc atgaaatggc cgcgggttta accatggctg ttgaaaatat
    28021 tgaagcgttt agaatgtata ttaatgaata tgctaataat aaaatggatg aagaaacctt
    28081 aattcctaaa ctatatctgg atgattgcat tcatatttca gaagtatctt tggaacttat
    28141 ttcgcagctt aaaaaaatgg agccttttgg gatgggcaac ccacagcctg ttttttctat
    28201 agaaggagaa ataagtaatc tttctctttt aggaaaagaa aaacaacatt taagaatcgt
    28261 tctggaagat tccaatgaaa ccattgatgc ggtaggtttt ggctttggtg agtattatga
    28321 caagctttta ccagggcaga aagttcttgc tgctggctgc cttgatatca atgagtggaa
    28381 taatacggta aaagctcagt taatgattaa agatttaaaa agcaccatag aagacgaaat
    28441 caaatttcat tactattttt ctttgtataa attattttta tctttaaatg atgaatgtac
    28501 taaaatagat tttcagtctt ttgaagagct ttctgcagat gatattttaa atgaagatag
    28561 tcttatttta attcaggatc tggaactttt attggagttg cttcgatatt ttggcaatgc
    28621 aaaaattatg tttaacaaca ggtctaaaat ttgttatact aataaatata gtttaaaaaa
    28681 caagatagaa attatcgtca atccgattat ggaaacgatt gatttttcag cttacaaaag
    28741 aattattata tcagatgcct tatgggtaaa taagactttt tcgattgcca aagataaaaa
    28801 agaacgtttt tttaaacttg aaaaaaagag atttagcagt cataaaagct ttagtccata
    28861 tagcttagag cgtaaagatt ttgaggtgct ctataggttt ttggccttat taagccgttt
    28921 aaaacaaaat tcaattacaa tggatgccct tatcaaagaa tgtaataaat cgtttaagac
    28981 taatcctttt aagatacttc tgtcgttgga tatttttatg gaattgggtc tgattcaata
    29041 cgaatataaa aatgataaac tttcttttga aattgtttct ggtaaaaaaa tatccttaga
    29101 ggattcaaag atcctgcaaa aattaaggaa attaaatgaa aaaacatagg agaaaacaga
    29161 gggggaagga aaaatggatt ttaaatcact tataagaacg ataccggatt ttccggaaga
    29221 aggtgttttg tttagggata ttacacccgt cttacaaaac agggaaggct taaaagctgc
    29281 tattgatcag atgcaggcct gcataaagga tatagaattt gaccttattg taggacctga
    29341 gtcaagaggt tttatttttg gagttccttt agcttataat ttaaataaag gttttatacc
    29401 tgttagaaaa aaaggaaagt tgccttataa aacgctgcaa aaagaatatg ccttagaata
    29461 tggtactgcc attgttgaaa tgcacgtaga tgccattcag ccaggccaaa agattgtcat
    29521 agtagatgac ttacttgcaa ctggtggaac ttctaaggca attgttgatt tagtaaaaga
    29581 agctggcgga gaagttgtaa agcttgttta tctgattgag ttagaagcat taaaaggcag
    29641 agaggcttta aaaggataca atgtagaatc aataattaaa tactaataaa agacactttc
    29701 tttttaagaa agtgtctttt atcaatttaa agccaggctt caattaagct ttatagaaag
    29761 aggtaaaaga atggatattc ctattctgta tgaagacgga catatcattg tcgctttaaa
    29821 accaccaaaa attccttccc aggctgacaa aagcggggat aaagatatgc ttagttattt
    29881 aaaagaatat ttaaaagaac aatatcctat ggctcagaat ccctatattg gacttgttca
    29941 ccgcctggac agacccgttg gcggactgat ggtatttgct aagagcaaat gggcgaatgc
    30001 aaaactctcg gagcaaatac aaaataaaca gtttaataaa gaatatttgg ctgtggtaac
    30061 cggaaaacct gaaaaagtac aaggagagct tgtggattat cttttaaaaa atcaaaaact
    30121 caatttatca aaagtggttt caaaggagac aaaaggtgcc aaagaagcca ttcttgaata
    30181 tgagtgttta gacagtgttg aatttgaaaa tgaaggatgg ttaagcctgt taaaaattgt
    30241 attaaagacg ggcagacatc atcaaatccg cgttcagttg tctcatgctg gtatacctgt
    30301 ttggggagat aacaaatata accctgcttt tgtaaaaaga agagaatgga cccagattgc
    30361 tttatgggca tacagtttgt ctttttatca tcctaaaacg aatcaattgt gtttatatat
    30421 agaaaaacct gaacagtatc cctttaacca gtttaacatg tagtttgtaa gaaacgatta
    30481 ttttccgtga aacagatttt ttgacaatct tgcatttagc aatgataatc aatataataa
    30541 aaagaaatgc ttattgcaga tatgattctg tattgtgaat gggggaagag tatgtctgac
    30601 caaattattt atgaaaagtt aatatctctg cttaagtcat atcatccctc taatgatttt
    30661 tcgttagttg agaaggctta tcgttttgcc aaagaagccc atgaaggtca gcatcgaaaa
    30721 tcaggagaac cttatgtaat tcaccctctt gaggtagcgg ttattttggc agagttagaa
    30781 ttagatatag aatccattac tgccggaata cttcatgata ttctggaaga tacggatcat
    30841 acttttgaag aattggcagg tctttttgga gaagaagtgg cacggctggt ggatggcgta
    30901 accaaattag gtaaaattaa tttttcttca aacaataaag aattgcagaa agaagaaatc
    30961 caggcagaaa actatagaaa aatgttttta gccatggcac aggacattag agtgattctc
    31021 ataaaattgg cagacagact tcataatatg agaacccttc gctttatgcc cccggaaaag
    31081 caaaaagaga aagctaatga aactttgcat atttatgcac ctttagcaca tcggttaggt
    31141 atttcgaaaa ttaaagtaga acttgacgat ttggctttaa gatatcttga gccagaagtt
    31201 tattatgacc tggcagaaaa aattgcaaag cgcaggatag aacggataga gtatgttgaa
    31261 cgcattgtaa aagaagttaa aacaaaatta gatgaagcag gcataaaagc aacagtagaa
    31321 ggaagaccaa agcacttttt tagtatttat aaaaaaatga ttggtaaaaa tgtcacatta
    31381 gatcaaatct atgatttatt tgctattcgt gcaattgtgg aaacggttaa agactgttac
    31441 ggggttttag ggattgtcca tgaaatgtat aagccaattc ccggaaggtt taaggattat
    31501 attgccatgc ctaaacccaa tatgtatcaa tcccttcata caagcctaat cgggcctgaa
    31561 ggggaacctt ttgaagtgca aatccgaacc agagaaatgc ataaagttgc agaatatggg
    31621 atagcagctc actggagata taaagaaggc aaaaaaggaa cgaaggttac tgacaaatct
    31681 caggataaaa cagaagaaaa attggcatgg ctcagacaaa ttttggaatg gcaaagagaa
    31741 atgtctgata acaaagaatt tcttgatgct ttaaaaatgg acttagatat atttagcgat
    31801 caggtatatg cttttacccc gaaaggagac gttattagtc tgccttatgg ttcaacgcct
    31861 attgattttg cttatatgat tcatagtgcc gttggaaata aaatggttgg tgcaaaggtc
    31921 aatggtaaaa ttgttacctt tgattatcaa ataaaaaatg gagacagggt tgaaatttta
    31981 acttcccaaa attcaagagg accgagcagg gattggctta aaaacgtaaa gagttcccag
    32041 gcaagaagta aaattaacca gtggtttaaa aaacagtata agcaggaaaa tattaaccgt
    32101 ggtaaagagc ttcttgaaaa agatgcaaaa aaacggggtt taaatataaa cgatttatta
    32161 aaagccgaat ggatagaaaa agttttaaga aaatatggtt ttcaggactg ggatgccgtt
    32221 tgtgctgccg taggtcatgg cggtattaag gaagggcaag tgattcaccg cttatatgaa
    32281 gaatacagaa aagaaaatgc gaagaatttg attaaagaag agacgatcac cattcaggat
    32341 gacagtaaaa aacctgttaa aaaatcaaaa agtggcgtag tgatagaagg tgttggagac
    32401 gtggcagtgc gtttttccaa atgctgcaat ccagtacctg gtgatgaaat tataggtttt
    32461 attacaagag gaagaggtgt atcgatccac agaaccgact gtatcaatat tcttaatatc
    32521 agtgaagaag aaaaagaccg cttaattgag gctgagtggc ttgttgccga gaaagatttt
    32581 aaaaatatta cttaccagac tgaaattaag gttattggag aagacaggat gggcttaatt
    32641 gttgacattt caagagtttt gacagaagaa aagatacctg taaaagccct caatgccaga
    32701 acaaccaaaa ataatgaatc gattattaat attactattg aaattagtgg tatacagcaa
    32761 ctggaaacca ttatcaaaaa gatcaggagt atacctggaa ttaatgatat cattagaaca
    32821 acgacttagg agggccatat gatattaaaa gttctttccg ttgggttaat tggtaccaat
    32881 tgttatattt gcggagacga agaaagcaaa aaaggcgtta tcatagaccc tggtgctgat
    32941 gcagagttca ttatttctga gttaaaaaaa gaaaatttgg acatagaata tattttgatt
    33001 actcatggtc attttgacca tattggtgcc ctggaaaaag taaaagaatt cacgggtgca
    33061 aaagtcctta ttcatgaaga aggtgtagct tatttagaag atgcttatct caatctttca
    33121 aaggcatttt ttgataagga gtttgtaaaa agtgctgatg gaactctaag ggatggggat
    33181 atgattgaag ttggaaattt atcttttaga gtcattcata cccctggtca tacttcggat
    33241 ggtgtaaact attatgagga gaaaaatggc atcctatttt ccggtgacaa tttatttaga
    33301 acttctatag gaagaacgga ttttcctgga ggcgatatgc atcttttggt ttcttcaatt
    33361 aaagaaaagc ttttgagttt accggaagat gttgtggttt atcctggtca tggagaacct
    33421 acaagtattg gttatgaaaa gcagaataat atttatttaa gtgaaaacag ttaggatgga
    33481 aaagatgaca ccttatgaat tagttggtca tacacacagt tatcagatag aaaatatttt
    33541 aagactttat gaaaaatata tagaagatat ttctataaaa agtattttaa aggaaaatat
    33601 ttgcagagct gaaatctata aaaataatca tctgctcatc tataaagaag agaaagacat
    33661 aaaagacccc aggctgcaaa agcaaaagct ggcctcagtc ttatatcagg ctttagtaga
    33721 aatgacagga aaagaaatgc cctggggttt tttaactgga atccggccta ctaaaatcgt
    33781 ccatgaaatg atggaagaaa atttaaccga tgaagaaatc aagaagcgct tgctagaatt
    33841 ttattttata agagaagaca aaatccaatt aatgatggaa gttgcaaaaa aagaaaaaga
    33901 aatcttagat aaaaataagg ataacgaatt aagtatctat ataggaattc ctttttgtcc
    33961 aacgagatgt ttgtattgtt cttttacttc ttatgccata gataagcggg aaaaacaagt
    34021 ggaagattat ttagatgctt tggaaaaaga aattttatcg agcaggaaat atttaaagga
    34081 taaattcatc aggagcctct atattggtgg cgggacacct acttctttaa atgctgcgca
    34141 gcttgaaaga ttattaattc aaattaataa atcttttttt atggatgaaa tagaagaatt
    34201 tacggttgaa gcaggaagac ctgatacgat tacgaaagaa aaattgaacc ttttaaaaaa
    34261 atatcatgtc aataggattt ctattaatcc tcaaaccatg gataatgaga ctttagtaag
    34321 aataggcaga aaacataaca aagaagatat tattaaagct ttttatatgg ccagggaact
    34381 tggctttgat aatattaata tggatcttat tttaggtttg cctggtgaag acagaaaaaa
    34441 cctttcttat accatgagcc agattcaaaa attgtctcct gacagtataa cggttcatac
    34501 tatggccata aaaagggctt caaggcttaa ggagactttg gatgattatt ctctcattga
    34561 tgaaaatgaa atagaaaaaa tgattgagat gagttatgaa tcagccatcc aaatgggaat
    34621 gcagccctat tatttatata gacaaaaaaa tatgcttggc aattttgaaa atgtaggtta
    34681 tgcaaaagtt ggtaaagaat ctgtttataa tattcaaatc atggaagaaa aagaaagcat
    34741 tcttgctctg ggagcaggag ctattaccaa gcttgtagat aaagaaaaca ataagataga
    34801 gcggattgcc aatgtgaaaa atgtagatga gtatataaaa agaattgacg aaatgatact
    34861 tagaaaagaa gccgggatgg atttttttga aaaatagttt ttataatttt ataatttaag
    34921 ttaaaataaa tgttaaagta aattttaaaa taagtggatt tttatggctt taaacataag
    34981 gaggtaaata tatgttgaca aaagctcctc ggggaacaaa agatttattt ggggatgaaa
    35041 tgaatgcatg gttggaaatg gagaaaataa taagggactt atgtaaggat tttggttatg
    35101 gtgaaatccg gactcctgtt tttgaacata cagagctttt tcaaagaggc gttggagaga
    35161 cgactgatat tgtacaaaaa gaaatgtata cctttcagga taaaaaaggc agaagtatta
    35221 ctttaaaacc agaaggcact gccggggcag tcagggcata tctggaacaa aaattatatg
    35281 caggtattca gcccaccaga ttatactata ttaccccagc tttcagatat gaaaagccgc
    35341 aggcaggtag aatgcgacaa tttcatcaat ttgggattga acttttaggt tctgattctc
    35401 caacggcaga cgcagaagtc ataacggttg cctatgaact tttaaaacgt gtgggaataa
    35461 gtaaagttaa actgcatata aacagtttag gcggaccaga atgcagaaaa aaatacaatg
    35521 aaaagttgaa agaatatctt aaggacaatt tagatcatct ttgtgagact tgcagggaca
    35581 ggtttgaaag aaatccttta agaattttag attgcaagac agaaagctgt aaaaaaattg
    35641 tttctgatgc ccccacggtt ttagatgttt tgggcgatga atgcagggcg cattttgaaa
    35701 aagttaaggc cttattggat gaaatgggga ttccttacat tatagatccg ggcattgtaa
    35761 ggggattaga ttattataca aagactgttt ttgaatttat ttccacagat attggtgccc
    35821 agggaaccat ttgtggtgga ggcagatata ataacctggt tgaagagttg ggtggacaac
    35881 caacaccagc cgttggcttt gccgttggtc tggagcggct tttactggtt atcgaagcag
    35941 aaaatggcaa gaagacctat ccggtaacaa aggatatttt tattggttct ataggagatg
    36001 ctgctctcct taaagcccat agtttaatat atcagctcag acaagctggt gttacggctg
    36061 aaggagacca ttttaataaa agtgttaagg cacaaatgaa gtatgcagat aaaattgggg
    36121 caaaatattc tatgattatt ggtgatgatg aaatcaataa tggaaaagcc attcttaaga
    36181 atatggagac aaaagaagaa agagaagttc atctcgatga acttgtaacg acgatgaaat
    36241 ctatacttta atgattgaat ggaggagtat tatgggagaa tcaatgcttg gcttaaaaag
    36301 aagccattat tgtactcagg tgaatgaaag tcttattgga caagaagttg ttgttatggg
    36361 ctgggtgcta aaaagaagaa accttgggag tttgattttt atagatttaa gagacagaac
    36421 tggtattttg caaattgttt ttgatgaaaa agacttaaat gcaaacgatt ttagcaaggt
    36481 tgagagctta agaagtgaat ttgttattgc agtaaaaggg ctggttcgta acagagaaag
    36541 cattaatccc aatttagcaa ctggaatgat agaagtggtg gcaaaagaat taagaattct
    36601 ttctgaagca gaaactccgc cttttgccat agaagaaggc agtagtgtaa aagaagattt
    36661 aagacttaaa taccgttatt tagatttaag aagaccagat cttcaaagaa atttaatgct
    36721 gcgtcataaa gtggcgcagt ctgtaagaaa gtttttggat tctgaaggct ttcttgaaat
    36781 agaaacccct atgctggcca gaagcacacc agaaggggca agggactatc tggttcccag
    36841 ccgtattcat aaaggaagtt tttatgcctt gcctcaatca cctcaaacct ttaaacaatt
    36901 attaatggtt tcaggttttg ataaatattt tcagattgta aaatgtttca gggatgaaga
    36961 tttaagggca gacagacagc cggaatttac acaaattgat atggaattat ccttcgtgga
    37021 tgtggatgat gtgattgatg tcaatgaaag gcttctaaag actgtattta aagaggccct
    37081 tggtattgat attccccttc ccattccaag attgacttat caggaagcta tggaccgctt
    37141 tggcagtgat aaaccagata tccgttttgg tcttgaactg gttgatattt ctgatgtcgt
    37201 acaagattgc ggctttaaag tgttcgaaga ggctattgcc aatggcggca gtgtacgcgg
    37261 tattaatgca aaaaactgtg cgaacttttc aagaaagcaa atagatgctt tggtggaagt
    37321 tggaaaaact tatggagcca aaggaattgc ctggcttgct attggtgaag acggcaccat
    37381 aaaatctact tttaccaagt ttttatcaga agagatttgc caggaaatta tcaaagctat
    37441 ggatggtcaa aaaggtgatt tgcttctttt ctgtgccgat aaaaattctg tcgtatatga
    37501 tgctcttggc aatcttcgtc ttgaaattgc taaacgttta aatttaatta ataagaatga
    37561 atactctttt gtatgggtaa cagaatttcc tttattagaa tgggatgaag aagaaaacag
    37621 atatgtagca aagcatcatc cctttaccat gcccatggat gaagatattg aattattaaa
    37681 tacagaccct ggaaaagtaa gagcaaaagc ttacgatatt tgcctgaatg gcgttgaatt
    37741 aggcggaggc agcattcgta tttaccgaaa ggatattcag gaaaaaatgt ttaatgcttt
    37801 aggttttaca aaggaagctg cttatgaaaa ctttggcttt ttattgaatg cctttaaata
    37861 tggtgtacca cctcatggtg gtttggcata tggctttgac agaatgatta tgttgatgac
    37921 gcaggcagac agcataagag atgttatcgc attcccaaaa gtaaaagatg cttcttgtcc
    37981 tatgacagat gcaccgggaa aagttaccga acagcagttg gatgagttgg gactaaagct
    38041 tgatgagaat aatgaataaa aattcttcgc ttatgcgaag aattttttaa gtctcgtata
    38101 aacagacttt tttacaggaa atgagtatat ataattaaat aatatttctt atataataaa
    38161 gataaagatt tttattttac tgccttatgt aaggaagata caggaggtaa tcatatgagt
    38221 aacaggaaaa atcaaatatt ggtaggtctt attattgttg cgatggggct gtttacttta
    38281 tttgggcaat ggattagtat tccctttatc cggaaaaata atttatttgg cttgtttata
    38341 gcaacgaccc ttttactgct ttattgtaca aagaaaaaaa cctgggccct tgttgcaggt
    38401 atgtttatag gtttttttag cgtcctgggc attttcccaa atgtaaattt taataccgct
    38461 actttcattg ctccaatggt ttttattttg ccaggtttta tttttatcat attgtattat
    38521 tctaaataca atattggttt tttaattcct ggttcgattt taatgtggtt tggtatattt
    38581 atttttttag taaccagcgg tttgatgcgg ggaatgatga ttccagttgc ctggtttgga
    38641 agtctggggg cagctttctt atccatgtat ttattaggtc ggcataagat aggaaagtgg
    38701 cctttgattc caggaggaat cttgctggga atggcatttt tattatttgc aggcgcatct
    38761 ctgggcttta tcttcaggtt aggacctaag ttaattcctg ttactctgat tattattggt
    38821 ttattcattg ttattaaaag cagaaaaagt aattaaaagc tgtagcatat cttaacacct
    38881 ttccaaaaat gaataattga caaagaactt tgctgattgt aaaattaaca tctgaaggag
    38941 agatgttatg ctacatgctt tttcaagaac tgaattatta ttaggtaaag aagccatgga
    39001 gaaactatac aaaagcaaag ttgctatatt tggcattggc ggcgttggaa gttttgttgt
    39061 agaaggttta gtccgttctg gtataggaaa gtttattcta gttgacgatg actcggtatg
    39121 tttaaccaat ctcaaccgcc aattacatgc gaccagaaaa acggttggaa gagcaaaagt
    39181 agaagtgatg aaggaacgga ttttagaaat caatcccagg gcggaagtta aagttcatca
    39241 gacatttttt cttcctgaga catctgaaga aattattgat gatgattgtg attatattgt
    39301 ggacgctatt gatacagtaa cgggcaaaat agagcttgtt ttacaggcca aaagaaaaaa
    39361 tatacctgtt ataagttgtc tgggagcagg gaataaactg gatccgacta aatttgaagt
    39421 ggatgatatt tataatactt ccatatgtcc tttagccaaa gttatgagaa gagaattaag
    39481 aaaacgagga atagaatctt taaaagtggt ttattcaaaa gaagttccta ttaaacccaa
    39541 agaagaagaa gtgatgactt gcagggaagg ctgtatttgc agcaataaag aaagaacttg
    39601 cctggaaaaa agacaaattc caggaagtgt ttcttttgtt ccatctgttg caggtttaat
    39661 tattgcagga gaagttgtaa aagatttgat ttccaggtaa tgatgataaa aggtggtttt
    39721 gctttatgaa gtatagaaaa agaggagaag ttggtcaagt catagatgtt aagcaaaata
    39781 ttgccattat cagtatgacg agaaatgaag cttgtcagca atgtggtgct tgtagtcatg
    39841 gacaccggag tgaagaaatg atattagaag caaaaaattt atgcaacgcc aaagtaggag
    39901 acaaggttgt catagattta tcttcttctg attttctaaa agcaacgttt attatgtatg
    39961 gcttaccttt aattttattg tttacaggat ttattatagg ttattatggt tcttataaat
    40021 taggttatac gtccttgcag gagccaatgg gtgtattatt tggttttctt tttatgacat
    40081 tttcttattt ttggataaga gccaacgaag aaaaatggga aggtcaaaac taccgtccag
    40141 tagctgtaaa aattgtagaa gaataaaaat aaaatgtaaa cctcctttta tttataaaat
    40201 gaaaaaggag gttttcttta ttagctattt tcttcctcaa aactaaatat ctttttaaag
    40261 atagaaggtt tattgccttt ttctttttta ttttccccac tgtctgttgt aataatcagg
    40321 ggggcttttc tggtttctaa ttcttttttt aatatatcca tccctttttt gagggcagta
    40381 tttttttctt caagttgttt gattttttca tttttcttag tcagttcttc ttcaagtttt
    40441 tttatccgct cttttagtgc agtcaattct tttttttgct gttcatttat tttgctaaag
    40501 tcgtaagccg ttttagaaat atctttattt ttaactttta aagaatattt caattggtta
    40561 atttggtttt ccttttcccg taattgcttt ttacaaatat agatttcttc ataaagttga
    40621 tttaagtgaa ttttaatttc attcaagtaa ttatcgactt tttctgttgc ggggataaaa
    40681 gtatgattaa tttggatact tgttccattg attgcaggaa aaacttctgg caggataaga
    40741 aattttattt gaggatttgt aagaatatag atttcattac ataacaggcg tttttcttct
    40801 tcaggaaagt tactttgata atgggcttta aggataaggg caggttcttc tatcagatga
    40861 atagctgctt ctgaaaaact gtttcccctg tcagtggaat aacagctata gagtttgtct
    40921 tgacaaatcc aggctgccca aagatgatcg tcaatcataa acaaggaaca tgagcttata
    40981 tcggcctttt cgaaaagttt ttttgcctct ttccactgat tatccgtttt tgacctgaaa
    41041 tagagggttg aatagtgtgc ttctttttta atgtatagat taaaaagttc atcttgactt
    41101 gccagtatgg attcatctgt aaaggaaaaa tgacttttat caaagttcag aaaatctgac
    41161 cagtttttag aggaagctga atatttttta taacccagtt gatagtactc actgtatttt
    41221 tgataagaaa taaaaatgtt atcctggtta tctttgtaga ctgtaaaggg ttttttatcc
    41281 aggggataaa tgtagtcaat aagggcaggg gttttccatt gctttccgct ggtggctttt
    41341 tgctctacta aggcatgcct gttgctgttt ggatctttta agttataaaa caagtaaaag
    41401 ttatcatttt ctatgatggc atcaaaagaa agagagtcaa taaaaccatt tttactgtaa
    41461 agcagaacct gagattccca tttgcctgct gtgttttggt aaaggataat attaccggaa
    41521 ctatcctggc aataaaggta tatattgtcc tgcttatcca gataaacaga aaaattttta
    41581 cttccgctgc tgtaaagcct ttgttttttt gaccagtggt cattgtcaca ggtcatgtag
    41641 cagataccct ctgaatcgtc ataaaagaaa gcccataaat tacctgtact ttgttttata
    41701 atgtggtagt ttattctttc ttttgtcatg gaagccctcc attccgccta tttggctgaa
    41761 ttttttatat tataagcttg tattaaaatt gaaatttaga aaaagcaatt taaactaggg
    41821 caggacaaga tctgccctaa ttcatttata tgatttgtaa aaaagaaata atactattta
    41881 tatttcttct ttttcttttt tttcatcttc tttaggaagc tcaacctgtt tctgattttc
    41941 ggtacttatt tttgcttctg agattttttc taattgctta attcttttct tgtgaaattc
    42001 tatttctgat tttgcttctt tatatagatt acgccacttt ttaccttctt cttgtatttt
    42061 tcttgctaat tctgccagtt cgttatattg atggcttata tccatttgaa tatttgtcat
    42121 cttttctaac tcgtctttaa gacgatgatt ttcttcttcc aattttcttt tagatacaga
    42181 aatatttaat tgctttttaa tatgtgtaag atatagtttc aattcattat tgacctttaa
    42241 atgaggatgg atgccgtcca tatccaggtt ataaagaaga acaaattgag ggggataatg
    42301 actgataccg taaagctggt tgcaaattaa tgggctgtca gagagcttgt tctcataata
    42361 atgtatgctt gcaccagaat cttgtataga attgtgaatt gtttctgaaa aactatctcc
    42421 catatcaacg gataaaaggg atttaaaagt accattttca ttccagttaa tccataaagc
    42481 atcttgataa atgaataaaa ccggcttaat aaaataacca caggaatata aagggtgact
    42541 gttggaccag ccttctttat tatttttata aattaactga tattgtccat attgctcctg
    42601 aatatagagc aagtggcaaa taccagattg atccaaaatc aatttacaat actttacagg
    42661 aaattttgat tttactggct gataaaaatc aacccattta tttgactgag gatttaaagc
    42721 tttaatgttg attgtataat cgtcttgctc tttactaatc atagaaagca aaacattatc
    42781 tttttgtctg atgcaatcaa aattaatttc acggttaggg atttcaccta aggggacagg
    42841 agcgcaatag ggctttttaa gcagttgata gattagttct gtatttttgg tagaagggtt
    42901 atcggcatag taaagcaaat ggacctggtt tttgaaggat aaagcgaata tatcgctaat
    42961 tttaaattca ttggcgtaat cgtccagaat gatttctttt tctattttat ttgataaaag
    43021 ttgaaaatgg atgatttgac ttttatcatt ttttgcaatg acatgtaaat tactattctc
    43081 atctaaaaaa gcggaatatt cataagtccc attggccagt aataaattag gacgagaagt
    43141 atgatcatcc ataaaaattt gataatataa gccttttctg ggactataat taaccattat
    43201 taaagtcccg tcaggatttt ttaaaaagca tgccatatta aacctcctaa actccttttt
    43261 atcatattat gtatattcag gaaaatattt tttatgttta gtaacacagt cgacaatttt
    43321 cacatagtat aatattgaat ttaaatcatt aaaaaaataa ggagggaatt atttatggca
    43381 tgttgtaata acaaccaggt aagaggcaga ttctttcaaa gacccacagc aggtgaatgt
    43441 cctgtatgca aaccagatga aatcataaat cctgcagaag acgcatgtgt ttgcccacca
    43501 ttaggagaac ctaaattatt aactgtagct gcaccagttg tatttgacga atgtggaatt
    43561 aatctttgca gagtagtgga taatttagat aaaatatttt gtaatgtgga tttcgaaaga
    43621 gacgatgttc aaggcattga attaaaggtt atcgacattg attttaattt ttgcaatgat
    43681 gatggctcta atgttgaaac cttagtaaga agacctaatt gtgtaagggt aaccttaaca
    43741 gaaatcgatg taacctttgc agctaaagtt atcgacagaa attgcagggt aattgacgaa
    43801 gaatgtttca cgattagata tttgcctggc aatgaagaag atccttactt tgacaatgat
    43861 gcaaatccag tctcaatttc tgttgatatt tatgcacctt atggcgtttc ttacaactta
    43921 aacggagatt gcactcctgt aatcagcttt ttaggttttg ttgaaaatgg ttctgaagga
    43981 aacaatgcac ttcgtcaagg tcttgtggct caagctttag caaaggttgt taatctggat
    44041 attgattcct tatctgcagc gattggctta accatttatc tgaaatctat ctacttcgtg
    44101 caatataaaa tacctcataa gggtctctgt gttccaccta aatgtattcc agtggatatt
    44161 gaagcagaaa atgcatgtct tgactttgtt gaaggagatc ttcttgaaca aagcattcaa
    44221 cctcttgaac tttgctgcaa gcctaaatcc attaaacatg gattttcagc agtagaagat
    44281 cctactcctg cagaagaatg tggaaagaaa agatcataaa cgggcctgaa aagacagata
    44341 aaaatacact tctcaaattt gagaagtgta tttttatcta agttttataa tacttttatt
    44401 tcctgaaatt ttcttcaaaa tggcacatta tttttatcaa aacatattat ggtattgagt
    44461 aagaacataa aaatcaatta attaaaaaca gaattaaggg ggaaattata tgggtagacg
    44521 agacgacgaa tgtattattg ccttaaaggt atatgaccaa tgcagacaac aggactgttt
    44581 gacacctgaa atgctggata gagtattctc gttagaatct ggagaatgtg ttgtaggtga
    44641 tcaaatcatt gctgcaggaa gaggctctat tattaatcta ccagtaggaa cagtggatat
    44701 tgctgtaaaa gactttaaga tttcaaaaat tgaaacggac agaaaacaag atgtatttaa
    44761 tccagactac tattttgtac atgtgaagta tttctttaag tttaaactta gctttttaga
    44821 tggcgatgga tgcccgattg ccattacatg cgacggtgtt gaaagagatg ccatggaagc
    44881 aggcactgct ttcaagaaaa cagtaaaatt gtatggaagt gaaggttcag acattgctat
    44941 ttccagcaat ttatttgctc cggaatctca tgttttggaa gctgcacctt atgtattggt
    45001 agaagcaaaa gctatgcttt tagatgcaca aattgataca aacgatggct taacttgtga
    45061 gtgcggcgag gaagcaatag gagaaattaa tttgaccatt ggcttattta caattataaa
    45121 actatatagg ttggttaatt tattggttga atccagtggc ttctgtgaac cagaagaatg
    45181 tgatgaagtt gcttctgtgg atccatgcag catttttgaa aatatggatt tcccatttga
    45241 tatttttaat ccgcctcaaa aaaatgatgc acgatgtgga agacgcggtt ccggttctga
    45301 cagagacgat tatgaggaag attaaaagta attagtattt ataattttat gaatgcctga
    45361 acaatattat gcataatttg caaagaataa gggggtatta atttgagtaa gtgtagagaa
    45421 cacaggagag actgtatcga cgataaaaaa tgcattatat ccaatgccat taacgggctt
    45481 caaaaaactt taaattcatg ctgttgctgc gttataagaa aattagagga acttatcaat
    45541 gaacaaagat ctgctttttg tatcgctaat tccgtttgtg aagcagcaga agaaggagac
    45601 gctgagattg ctgtaatat
//
LOCUS       DAOSUD010000007        43032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_613_length_43032_cov_23.940084, whole genome shotgun sequence.
ACCESSION   DAOSUD010000007 DAOSUD010000000
VERSION     DAOSUD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 43032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43032
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_43032_cov_23.940084"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLG49_01910"
     CDS             <1..595
                     /locus_tag="PLG49_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOQ16099.1"
                     /translation="LGRALHQRGYFFSVSVPSRVSDEPFNPFSDPFQYAPIGAAVDEF
                     VVMLYNEHGWPGSGPGPGPVVSIGWMDRVLKYTMTKMPRDKIVAAVSVFGFDFNLDTG
                     RNTYVTYQMAINRAQQYNAQIIFDEVTQTPMYSYTDEQGQRHEVWFEDAKSIRAKVQK
                     AWDLGIKGIALWRLGMEDPAIWTMLRDETVVRKDIGL"
     gene            complement(639..1487)
                     /locus_tag="PLG49_01915"
     CDS             complement(639..1487)
                     /locus_tag="PLG49_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOQ16100.1"
                     /translation="MEKTGLVLEGGGLRGMYTAGVLDFLIDKKLFFPYIIGVSAGACN
                     AISYISRQRGRNRDINLNYIQDKRYISLSNLLREKELFGTKFLFDDIPNKLCPFDYDS
                     FQQAKETFVVCTTDCVSGKPMYFYKDNSEDFFEAVKASMSLPFISHIVEFRNHYLLDG
                     GITDAIPIKKSIEDGNTKNVIVLTRHKGYRKRPSKSHKFAQRFYADYPNLVEALDKRH
                     IMYNETLDYIEQLENEGKAFVIRPSTPVKVKRIEKNPKKLAALYEQGYQDIQNLYGSL
                     MEWLNH"
     gene            1651..2214
                     /locus_tag="PLG49_01920"
     CDS             1651..2214
                     /locus_tag="PLG49_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ16101.1"
                     /translation="MTRDEAYELLKKYTESESLIKHALAVEAAMRGYAKKFNEDVEFW
                     GITGLIHDLDYELYPDKHPLEGVKILEENGYPVELIEAVKGHADHTNTPRQTLLAKTL
                     YAVDELSSFIIAYILVRPNKSFEDIQLKSIKKKLKDKAFARGVDRDIIKKGAEELGVD
                     LDEHIMTVADALVEREKELNQMGVSLV"
     gene            2327..3517
                     /locus_tag="PLG49_01925"
     CDS             2327..3517
                     /locus_tag="PLG49_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOQ16102.1"
                     /translation="MSNMEWIKRGNKVIMNTYKRFPLALTEGEGVYLKDIEGKKYLDF
                     ASGIAVNSLGHGNKTLIKALEEQMNKIMHCSNLYWNLPAIEAAEMLIQNSIFDKVFFC
                     NSGAEAIEAALKLARKFGKKRHGEHCYEIITMKNSFHGRTFGAITATGQTKYQKDLNP
                     LMDGILYADYNDFESVKKLVSDKTCAILLEPIQGEGGIRPADKDFLLKLRDLCNEKEI
                     LLMYDEVQCGIGRTGYLFAYEAYGVAPDVISLAKGLGGGFPIGAMMAVEKYSDLFGPG
                     DHASTFGGNPAACSAAKVVLTALIQEGILENVKAQGAYLRERLNILKEKYTIITDVRG
                     MGLMQGIELSLPVANIVELCIQKGLLLVGAGEKVIRFVPPLIVGKNEIDEAINILETV
                     LKEV"
     gene            3568..4509
                     /gene="argF"
                     /locus_tag="PLG49_01930"
     CDS             3568..4509
                     /gene="argF"
                     /locus_tag="PLG49_01930"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOQ16103.1"
                     /translation="MGLNLRGRSFLTLKDFTPAEIDYLLDLAENLKRKKKMGIQGDLL
                     RGKNIALLFEKPSTRTRCAFTVACIDEGAHPEYLGKEDIQLGSKESVEDTARVLGRLF
                     DGIQFRGFKQETVETLAKYSGVPVWNGLTDKYHPTQVLADFLTIKEKFGCLKGIRLVY
                     IGDGRNNMANSLMIGASKMGMDFVICAPKSLWPEDILVEECKAFAKESGAKVTITDDI
                     DSAVFGADVIYTDVWCSMGEEAKAEERIKLLKAYQVNADLMKKTGKEETIFMHCLPAV
                     KGNEVTEEVFESKASVVFDEAENRMHTIKAVMVATLS"
     gene            4648..5730
                     /gene="serC"
                     /locus_tag="PLG49_01935"
     CDS             4648..5730
                     /gene="serC"
                     /locus_tag="PLG49_01935"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOQ16104.1"
                     /translation="MDRVYNFSAGPSMLPLEVLQKAQKEMLNYENSGMSVMEMSHRSK
                     VFDEIIQGAEEVLRDIMNIPHNYKVLFLQGGASTQFAMIPLNLFRNSKKADYVNTGQW
                     SKKAISEAKKYGTVNVVASSEDTTFNYIPDLDKSTFSPDADYFHITSNNTIYGTRYVE
                     LPDTGDVPLVSDMSSCILSEPCDVTKFGVIYAGAQKNIGPAGLTVVIIREDLIGHAMD
                     ITPTMLNYKTHADNNSLYNTPPTYGIYIAKLVFEWIKEKGGLAEMEKINKEKANILYD
                     FLDNSSFYKGTVVPKYRSLMNIPFVTPSKELDEKFVKEATANGLVNLKGHRTVGGMRA
                     SIYNAMPIEGVKALVEFMKKFEMENR"
     gene            5741..6916
                     /locus_tag="PLG49_01940"
     CDS             5741..6916
                     /locus_tag="PLG49_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HOQ16105.1"
                     /translation="MYKIKTLNKISEVGLQHFDRNKYEISEDCPNPDGIIVRSAKMHD
                     MELPASLKAIARAGAGVNNIPVDLCSEKGIVVFNTPGANANAVKELVIAGLLLSSRKV
                     VDAISWAKSLKGKGEEVPKLVEKGKSAFAGPEIKGKKIGVIGLGAIGVLVANAAKSLG
                     MEVYGYDPYISVDAAWGLSRAVHKADSLEEILADSDYITIHVPLLEDTKNMMNKERFA
                     LMKKGARLLNFSRAELVNTADLLEAIKEGIIACYVTDFPTDELLDVENVIAIPHLGAS
                     TPESEDNCAVMAVQQMMDYLENGNIKNSVNFPDCEMTRTTDYRTVVAHKNIPGMVGKI
                     TTTIANDGVNIMDMMNKSKKECAYTIIDSDKPVSDEVVEKLIHSDGILTVRTLNRIK"
     gene            7034..7870
                     /locus_tag="PLG49_01945"
     CDS             7034..7870
                     /locus_tag="PLG49_01945"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HOQ16106.1"
                     /translation="MNNTKIGFFTKLTRTIFDFNIYPHVIGESTGRAVAFLLLFSLLL
                     GIIGSLITSARYLLSGGLTVITDLMPDFEYDNGILTVEDMEEPFIYEMDSNKIFVIDM
                     NDEVDADILEYYSEGIMLKQDRVIAVQYGNIQEVSYTEFLDSYNIYHFSKEDLSKILP
                     VIDIGIVVIMIGFGVIGSVIGKMISSLFVSVLGLIVNAVSEAKLTYSDIYKTGVYALI
                     LPSLIKLILSSAGLNVGFIRMIFFVVYYGIVIFYLVKAITTIKRNKQEMMYQQYQQNM
                     MQ"
     gene            complement(7950..9395)
                     /locus_tag="PLG49_01950"
     CDS             complement(7950..9395)
                     /locus_tag="PLG49_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOQ16107.1"
                     /translation="MDYFVSIQLDRNSKIPLYQQLADALYDLISKDILKANTKLPTIR
                     KLANKLKVNNVTVINAYKYLEKKGLVYSQVGSGTYVSPIPVKDMPSPAYEEKKEIKQI
                     MITEDSINFASISPSTDLFPVESFKCIVNEILDKDKGNAFSYQESQGYEKLRESVADY
                     LKSYGINSSKDRIQIISGAQQGIDILSKALLQYGDTLLVEKPTYHGAIAAFQSRGARI
                     IEVPLETDGMDLEILENYLKIYHPKFIYLMSYYQNPTGYSYSLKKKRTILDLAEKYNT
                     YIIEDDYLSDLNYSEEKSIPVKALDHRNRVIFLKSFSKILMPGLRLGFLVVPKNLLDN
                     ILSAKQTTDITTSGFIQRVFDLYLRKGLWKSHINEIRKIYKKRYIYLMKLLDEHLKNE
                     ISYIPPDGGLSLWIKLKASISVDKLSDILLSRNVIISPGSLFSLSKESLNYFRLSFAA
                     VNEKQIHEGILILKEVLNNMNSDPASVEPIL"
     gene            9582..10394
                     /gene="pdxS"
                     /locus_tag="PLG49_01955"
     CDS             9582..10394
                     /gene="pdxS"
                     /locus_tag="PLG49_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOQ16108.1"
                     /translation="MSNKYDITESLKGGVIMDVTNAKEAKIAQEAGAVAVMALERVPA
                     DIRREGGIARMSDPKLIKEIKEAVSIPVMAKCRIGHFVEAQILEALDIDYIDESEVLT
                     PADDKYHIDKKLFRAPFVCGARDLGEALRRIGEGASMIRTKGEAGTGDIVQAVKHMRT
                     MMAQIRQLQNMAKEERMAFAKEIAAPVHLVEEVAELGRLPVANFAAGGVATPADAALM
                     MQLGSDGVFVGSGIFKSGNPAKRAKAIVEAVKYYNDPKKLAEISEDLGEEMN"
     gene            10577..11122
                     /locus_tag="PLG49_01960"
     CDS             10577..11122
                     /locus_tag="PLG49_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ16109.1"
                     /translation="MKELSLHILDITENSIRAKATEIYIEVEEDLNNNLFSFVIKDNG
                     KGMDEEFLKAVKDPFTTSRTTRKVGLGIPLLEAACKRCNGGLEIISVVNQGTQIKAWM
                     DYNHIDRAPLGDMVSTISNLIISNPDIDFVYCHKYNEKSFKIDTREIKNILDGVPIND
                     LSVINWLKNYIKDELTEIKQK"
     gene            11380..12705
                     /gene="rsxC"
                     /locus_tag="PLG49_01965"
     CDS             11380..12705
                     /gene="rsxC"
                     /locus_tag="PLG49_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOQ16110.1"
                     /translation="MQLLTFKRGIHPPDGKHLTENKPIEYLLPKGDLVYPMVQHIGAP
                     CEPIVKKGDRVLVGQKIGETKAYVSSPIHSSVSGTVKNIAPMLHPNGNKVLSVIVEND
                     GLYEEHESIGPKGDYKDLSREQIIEIIKEAGIVGMGGAGFPTFIKLSPPPEKKIDTII
                     VNGAECEPYLTSDYRVMLEETDRVVTGLKIILKLFPEAKGYIAVENNKMKAVEALLEE
                     TKNVDNIEVKVLKTKYPQGSEKQLIYALTGREVPSGGLPADVGCIVQNIDTVVAIHRA
                     IVRGRPLMRRIVTVTGGAIKDPKNFKVKIGTSYRELIEAAGGFVEEPVKIISGGPMMG
                     IALPSLDVPVCKGTSAILCLTREEAQLPQEQNCIRCGRCISACPMGLMPLELNRYAIH
                     KDIEQFETMHGMDCMECGSCSYICPAKRHIVQSIRTAKKAVLENRKKNA"
     gene            12718..13653
                     /locus_tag="PLG49_01970"
     CDS             12718..13653
                     /locus_tag="PLG49_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOQ16111.1"
                     /translation="MANGKYTVSSSPHIRSTDTIQSIMQDVFIALLPAAFAGVWFFRF
                     SALWIILISIVTCVGAEALWQKLTNQKITINDFSAAVTGLLLALNLPPTVPFWIPIVG
                     GVFAIIVVKQFFGGLGQNFMNPALAARVFLLTSWTPQMTSWTFDGMTTATPLGILKEG
                     GAQLPSLADLLIGRIGGCIGETSAIALLIGAAYLLYKKVISIRIPATFIGTVFVLTWL
                     LGRGGMFYSNALYEIFTGGLILGAFFMATDYASSPVTEKGQYIMGLGCGIITAVIRLY
                     GGLPEGVSYAILIMNLAVPLIERYTRPHYFGEVKQ"
     gene            13650..14216
                     /locus_tag="PLG49_01975"
     CDS             13650..14216
                     /locus_tag="PLG49_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOQ16112.1"
                     /translation="MKEILKLGLILFIITAVSGGLLGFAYEITKEPIAEQVRLANEEA
                     MVKSFPQGEKFKKIEAEIPEDSNVVEIHAVYKGNVLEGFVVKVLSKGFGGPIEMTVGV
                     SKDGMVQGVSILGHSETPGLGAKAQEDDFLSRYQGKSGILTVVKTGASKDNEIEAISG
                     ATITSKAVTDGINEVLDFIENHLDADNF"
     gene            14250..14855
                     /locus_tag="PLG49_01980"
     CDS             14250..14855
                     /locus_tag="PLG49_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOQ16113.1"
                     /translation="MKTIAERIKNGVLVENPTFVQVIGMCPTLAVTTAAKNGIGMGLA
                     TTAVLIGSNVFISLLRKFIPSKIRIPAYIVIIASFVTLVEFLMQGFVPALYKQLGLFI
                     PLIVVNCIILARAEAYASKNDILTSLFDGLGMGLGFTLALTVIGSVREILGAGQWFGI
                     QIMPSSYQPAIIMILAPGAFFTLGILMAILNYIRNRNTKKA"
     gene            14867..15439
                     /gene="rsxA"
                     /locus_tag="PLG49_01985"
     CDS             14867..15439
                     /gene="rsxA"
                     /locus_tag="PLG49_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOQ16114.1"
                     /translation="MEIFIIFLSAILVNNFVLSRFLGICPFLGVSKKVETAIGMGVAV
                     TFVMTLASFITYIVYHWMLVPLRITYLYTIAFILVIAALVQFVEMVIQKTSPTLYQAL
                     GVYLPLITTNCAVLGVAVININEGYNLIKSVINGFGAALGFTLAIVLFAGIRERMEHN
                     NILNAFKGFPIALITAGLMSIAFLGFQGLL"
     gene            15455..16270
                     /locus_tag="PLG49_01990"
     CDS             15455..16270
                     /locus_tag="PLG49_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOQ16115.1"
                     /translation="MDITSILYPVISIGGLGVLFGAGLGYASKKFAVEVDERIPQVRD
                     LLPGANCGGCGYAGCDAFAKAVVEEGVDICTCSVNSPEAIEKIAALLGVAAGCSIKKV
                     AQVKCKGTCSNSKNKYEYYGIMDCQDAAATPGGGAKACSYGCLGLGTCKKVCMFDTIR
                     IEDGIAVIDEEKCTGCGNCVEACPKAVIELVPQINQVRVVCNSKDKGKDVKEACSVGC
                     IGCSLCVKTCEYNAIDFKDNLAHIKYDQCTQCMACVQKCPTKVIAGQLELAEL"
     gene            16386..16646
                     /locus_tag="PLG49_01995"
     CDS             16386..16646
                     /locus_tag="PLG49_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOQ16116.1"
                     /translation="MNIKKGKNNWVLFIFILMGIVIGGFIGHYLGQFPYFQWLNFGKE
                     FGFQKPVALDLQIIYIEIALKIEITISSILGVVAAILIYRKI"
     gene            16689..17291
                     /locus_tag="PLG49_02000"
     CDS             16689..17291
                     /locus_tag="PLG49_02000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ16117.1"
                     /translation="MEKIILASASPRRKMLLNQIGVSFEVMVSDVEEKINYELPPDEL
                     VKELSRQKAMNVAKRLGEGFLVIGADTVVVFDGKILGKPKDKEDAFKMLFMLQGHWHE
                     VYTGVTIIKTKDMLVESDCEKTEVYMRKLSSSLINQYINTGEPLDKAGAYGIQEKGGL
                     FIDKINGDYNNVVGLPVKLIVALAEKMGVDLYQNIFKIRS"
     gene            17321..18001
                     /gene="radC"
                     /locus_tag="PLG49_02005"
     CDS             17321..18001
                     /gene="radC"
                     /locus_tag="PLG49_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOQ16118.1"
                     /translation="MSMKDLPKLERPYEKLEYFGPQALSEAELLAIIFRTGSRELRAV
                     ELAQRLLALEKEEGIYGLYQYSLDELMKVKGIGRVKAIQVKAVLELSKRISKHQASLK
                     YRINSPGSIALIYMEEMRYLKQEHFKIILLDTKNQIIGCQEVSKGSINSSIVHPREVF
                     KYAIKKSAVSIIMLHNHPSGDPAPSQEDIKVTERMKEAGELIGIHVLDHIIIGDGVYV
                     SLKERGII"
     gene            18252..19271
                     /locus_tag="PLG49_02010"
     CDS             18252..19271
                     /locus_tag="PLG49_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOQ16119.1"
                     /translation="MFFSRDLGIDLGTANTLVFVKGKGIIVNEPSVVAENKTTKEILA
                     VGDEAKQMIGRTPGTIVATRPLKDGVIADFATTEAMLKYFIGKAYKKGLFTGKPRVII
                     CVPSGVTAVEMRAVYDAARNAGAKEAHLIEEPMAAAIGAGLPVGDPTGSMVVDIGGGT
                     SEVAIISLGGIVTSKSLRVAGDEIDEYIVNYIKKEYNLMIGERTAEDIKMTIGAAFPK
                     EREESKEIRGRDLVTGLPKTLKITSTEIMEAIRDPINAIVDAIKYTLEKTPPELAADI
                     MESGIMLTGGGALLSGFDKKISQETGMPVRIAEEPLNCVALGTGLALEQIETLRNILI
                     SPKKI"
     gene            19299..20156
                     /gene="mreC"
                     /locus_tag="PLG49_02015"
     CDS             19299..20156
                     /gene="mreC"
                     /locus_tag="PLG49_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOQ16120.1"
                     /translation="MRRSFKIKEKYVIVIVTIVLLITIILTAGKRYNITFIEKGLGYI
                     VMPVQKTFSSVGEWISDQIYFIKNIKELEKQNTELAKKVDELSYENKILQQYKDENTR
                     LRKLLELDKKYPDYPKIGAEIIGKDPGNWYNVFLIDKGSNDGLEVDMVVLSGNGLVGH
                     IIEVAPNYSKVLSIIDDRNAVSSKVLRTGDLGIVKGDLTLLNEGLCKMEYVDAEADII
                     VGDEIVTSNLSDIYPPGIMIGVVKEIETESHGLTKYALIEPVVDFRHLEEVLVINMKW
                     NKKDDATKE"
     gene            20162..20680
                     /gene="mreD"
                     /locus_tag="PLG49_02020"
     CDS             20162..20680
                     /gene="mreD"
                     /locus_tag="PLG49_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOQ16121.1"
                     /translation="MRRILAITVILLLTNILQGTYFQGLEIRGIVPNFYIIIIVSFAL
                     LRGSKEGAIVGFFAGLLQDIYYGTSIGFYALLGVYIGYFSGKLNKSFYRESFFLPLIL
                     TIVSSFFYDMAIYIFTYLIRGRIQISYYFYNIILPGMVYTGVFSLFVYQGIYYINEKL
                     EIREKKYRNFFK"
     gene            20718..21404
                     /gene="minC"
                     /locus_tag="PLG49_02025"
     CDS             20718..21404
                     /gene="minC"
                     /locus_tag="PLG49_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOQ16122.1"
                     /translation="MINDNSVIFKGTKNGLTILLSPDIDFNILKNHLEKKIEDAKDFF
                     KDAKTSIMFKGRDLTEEEEYQLLQIITKKTELNISYIFDEETSIDMPEKIEKSLRSVQ
                     LNDEREQIDKTKYYKGTIRSGQCLEFDGSVVVIGDVNPGGEIYAEGNIIILGSLKGIV
                     HAGCKGYDKAFIAALIMKPMQLRICNIITRSPDNPDYSTHAKVFPQVAYLSDGNIYIE
                     PIDYKLLENL"
     gene            21521..22318
                     /gene="minD"
                     /locus_tag="PLG49_02030"
     CDS             21521..22318
                     /gene="minD"
                     /locus_tag="PLG49_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOQ16123.1"
                     /translation="MSQVIVVTSGKGGVGKTTTTANIGTGLALKGHKVVLLDADIGLR
                     NLDVVMGLENRIVYDLIDVLEGHCRIKQALIKDKRYSGLYLLPAAQTRDKTAVNPEQM
                     QKLCSQLKEEYDYVIIDCPAGIEQGFKNAIAGADKAIVVTTPEVSAVRDADRIIGLLE
                     ASELRNPTLILNRIRMNMVKRGDMMAMEDVVEILAVDLLGVVPDDENIVISTNRGEPA
                     VTNPDSLAGQAFRNIVERLLGNNVPLLDLDINEGFMDKIKKLFGIGN"
     gene            22334..22615
                     /gene="minE"
                     /locus_tag="PLG49_02035"
     CDS             22334..22615
                     /gene="minE"
                     /locus_tag="PLG49_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOQ16124.1"
                     /translation="MDLFSFFSRKPSSKDVAKDRLKLLLIHDRINCSPDLLEMMKTDI
                     IKVISNYMDIDEDELDIQIGQTKAEGSDGTVPVLYANIPIKNVRKVGNQ"
     gene            22668..23795
                     /gene="rodA"
                     /locus_tag="PLG49_02040"
     CDS             22668..23795
                     /gene="rodA"
                     /locus_tag="PLG49_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOQ16125.1"
                     /translation="MISNKYLKSFDIVLVFLVTALVTLGIIAISSAQHVNSGADPIYV
                     KRQILFFVVGLIFMLITAFIDYHFLGDLYIVAYIAGILLLISVLLFGDASKGAVRWLD
                     LGFTRIQPSEFVKIIIILFFSKLIDKRQQQINKFSTLLLLGILLVIPVLLIMKQPDLS
                     TSLVFVFIFLMLLYVGGISYKYIISAILIGIPLIFLILIYIQNPNQKLLKDYQRDRIM
                     AMIHKDNKEYADDYFQTEQSVHAIGSGQLYGKGLYKGTLNQLSYLPEPHTDFIFSVIG
                     EEFGYIGCISIIVLFFLLIVRSLWIAKDAKDLFGKLLIVGVVSMIGFQAFVNIGVVTA
                     LLPNTGLPLPFISAGGSSLWANMIGIGLILNVSMRRKKSFY"
     gene            23807..24202
                     /gene="mgsA"
                     /locus_tag="PLG49_02045"
     CDS             23807..24202
                     /gene="mgsA"
                     /locus_tag="PLG49_02045"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOQ16126.1"
                     /translation="MNIGLVAHDAKKKLMENLVIAYRHILSKHNLYATGTTGRLVEEA
                     ANVTIHKYLAGHLGGEQQLATQIAHNQIDMVIFLRDPVSRKLHEPDEEKIMRLCDIHN
                     IPVATNLATAELLIKALERGDLGWRDIVK"
     gene            24303..24794
                     /locus_tag="PLG49_02050"
     CDS             24303..24794
                     /locus_tag="PLG49_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16127.1"
                     /translation="MEKKRNIYSYHGRRNSRNRKNNSKDIFLNFIMKQFVVSTFLFLL
                     IIILNLLPLNITMNLMQQINERIYYSMTWDEAVETVKYTASRIPKVKNWLEATDETEQ
                     QRNSKSQESIDNNNDMLLEDSDIDTENKMDSTDHLEDVFDSNEDIFIIEPDLGESQEG
                     IIP"
     gene            24842..25234
                     /locus_tag="PLG49_02055"
     CDS             24842..25234
                     /locus_tag="PLG49_02055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ16128.1"
                     /translation="MDGIITSFFGERINPVLDKKEFHDGIDIAAPKDEPIVAVKSGIV
                     VKVGVSNTYGNFVEYKVEGGYIIKYAHCSEIFVKEGDYLEQGQIIASVGNTGLATGYH
                     LHYGISKKGDYIDPIAYVELPVLETLLE"
     gene            25329..27182
                     /locus_tag="PLG49_02060"
     CDS             25329..27182
                     /locus_tag="PLG49_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOQ16129.1"
                     /translation="MTKLRIADKILKQVEKPARYIGQEVNSVIKDKNKVDIRFAFCFP
                     DVYEVGMSHLGMQILYYFLNKREDTYCERVFAPWTDMEEKMRENHIHLFALESQDEIK
                     NFDFIGFTLQYEMSYTNIINLLDLAGVSPWSKDRKEDDPIVCAGGPCAYNPEPLADFI
                     DFFYIGEGEASLNQVLDIYKEHKKQGGTKEAFLEKLLDIEGIYVPRFYEVKYKDNGLI
                     ESFTPIHKKAKAKIKKQLVKDLDDSYYPSKVILPWIQTVHDRVNLELFRGCIRGCRFC
                     HAGFVYRPVREKSKEILIEQGKKLLKNTGYEEISLSSLSTSDYSAFKEFSEELLETCQ
                     KAGVNISLPSLRIDKFSIDLMNKIQTVRKSSLTFAPEAGSQRLRNVINKRITEEDILK
                     GSQFAFEGGWDRIKLYFMIGLPTEKEEDILEIKELAYKILDTFYSIPKEKRARNIKIV
                     VSSSCFVPKPFTPFQWEPQCRYEEFMDRQNLLKKSIKRKEIQYNYHDATISSLEGLLS
                     RGDRRVGQVIYYAWKYGAKFDSWTEKFNMAAWKKAFELTNIDPAFYANRKRAYDEILP
                     WDHISIGVSKAFLIKEHQKALDEKTTPNCREKCAGCGAKIFERGVCHEIQN"
     gene            27202..27858
                     /locus_tag="PLG49_02065"
     CDS             27202..27858
                     /locus_tag="PLG49_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOQ16130.1"
                     /translation="MKFIGHLDLLRLMQRCIKKAELPVAYSKGFNPHQLIYIALPLSL
                     GATSEGEYMDIELTEDSLMTETEIKDKLNAVFPAGLKVEKVSRVKDKAKSGMAAVDGA
                     EYKVFISKEKMPVYFNEKTEAFFEQKEIWIEKTGKKETKKINIKDKIYRYCVQEDKDF
                     WIIHLLLAAGSKDNLKLETLIEALYQYIALDYNGYDIDIHRVDIYSEVDNQLIPLSNL
                     "
     gene            27875..29302
                     /locus_tag="PLG49_02070"
     CDS             27875..29302
                     /locus_tag="PLG49_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOQ16131.1"
                     /translation="MFTDIIVDVGIHQIRVGVVENQKLVELYIEEDNKEKTVGNIYRG
                     IVKRILPGIQAAFIDIGKNKNAYLNLRENQFLGENGQQLKQGDHITVQVQKEAMGSKG
                     YKVTSHFSFAGKYCVLIPGEKSIGISRKINDEKERLRLKELFNSLNLDNYGIIFRTNS
                     LGKEEEELIKEIQYLKNQCEVIKQKERYVKAPALLYKELSFPFIAARDLMTKEIRHFI
                     INDKEMYEEILAFLDLMDASLKEKLVYKEEKNLFAYFLLETQIERALSRHIWLKSGGM
                     IIIDHTEALTVIDVNTAKYTGKKDMEKTILQTNIEAAQEIAKQLRLRNIGGIIIIDFI
                     DMKEEAHKEMLIKILEEELKKDRVKTSIFGFTKLGLVELTRKKTGPSLTSLLLGKCPY
                     CSGRGMVASVKFIADKIAKEIDYIFTETIYNHITIEANKDIIHWFCSGDYSYQQAFEE
                     RYEKKLEFIENNQLAIDQYKLIKKS"
     gene            29448..29759
                     /gene="rplU"
                     /locus_tag="PLG49_02075"
     CDS             29448..29759
                     /gene="rplU"
                     /locus_tag="PLG49_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ16132.1"
                     /translation="MYAIFETGGKQYKVQEGDVVIVEKLGLAAGEKVTFDKVLAVSKD
                     DKLSVGTPYVNNASVAASVVEEGKGKKVIVYKYKPKKGYHKKRGHRQPYTKIKIESIN
                     C"
     gene            29762..30100
                     /locus_tag="PLG49_02080"
     CDS             29762..30100
                     /locus_tag="PLG49_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOQ16133.1"
                     /translation="MLKVVFYLNKNKQLCGFEISGHAGYGKYGSDIVCAAVSTLAINT
                     VNSIEVFSSDPICIETDKKDGLMIFSLDEKAKAYPADETLLFLKSLELGLKNIQKNYK
                     KHILISYKEV"
     gene            30103..30381
                     /gene="rpmA"
                     /locus_tag="PLG49_02085"
     CDS             30103..30381
                     /gene="rpmA"
                     /locus_tag="PLG49_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ16134.1"
                     /translation="MLRMDLQFFASKKGVGSSKNGRDSISKRLGAKRADGQFVKAGNI
                     LYRQRGTKIHPGINVGRGKDDTLFALIDGRVKFERKGRDKKQVSVYPA"
     gene            30583..31863
                     /gene="obgE"
                     /locus_tag="PLG49_02090"
     CDS             30583..31863
                     /gene="obgE"
                     /locus_tag="PLG49_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOQ16135.1"
                     /translation="MFVDRVKIYIESGSGGNGSVSFRREKYVPNGGPDGGDGGKGGDI
                     IFEVDEGLNTLMDFRHQKHFKAERGEDGKKKKCNGKNGKDLIIKVPPGTIIKEAETGK
                     IMADMTGETKSKVLLKGGKGGRGNQHFATASRQAPRYAERGQEGKGYWVILELKVIAD
                     VGLVGFPNAGKSTLLSVLTNAEPKIADYPFTTLHPNLGVVRNPYGRDFVIADIPGIIE
                     GAHQGIGLGHDFLRHIERTKILLHMVDVASIEGRNPAEDVEKINFELKAYNEELLKRP
                     QIIVANKIDVMENEDNLNLLKETYETKGIKVIPISAATQKGLKDLLIEISKILDQVDE
                     EPVKFEEDFDYFEEPEEIKEPFTIKKISSSYYVVEGTGVEKVIGYTNFESEKGFAFFQ
                     KYLRDKGIIDALEKAGIQEGDTVKIYDLEFEYYK"
     gene            31893..32183
                     /gene="yhbY"
                     /locus_tag="PLG49_02095"
     CDS             31893..32183
                     /gene="yhbY"
                     /locus_tag="PLG49_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOQ16136.1"
                     /translation="MTSKQRAYLRSLANTIEPIFQVGKSGITPDLTQGIHEALEKREL
                     VKINVLNNCIMSAKEVAVILQERTNSEIVQVIGNKIVLFRQSKKKPRIFLSE"
     gene            32214..32849
                     /gene="nadD"
                     /locus_tag="PLG49_02100"
     CDS             32214..32849
                     /gene="nadD"
                     /locus_tag="PLG49_02100"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOQ16137.1"
                     /translation="MRNVFNGLEHPESIAIMGGTFDPIHYGHLVTAEAVRHKYNISQV
                     IFMPTGQPPHKKDQEISHSEHRYLMSVLATATNPYFNVSRMEIDRKGTTYTIDTIKSL
                     RELYGDETELYFITGADALHYIFTWKDTEELLKLCTFVAVTRPGYNKEKLIEAIEIIK
                     NNYESKIHFLEVPALSISSTDIRKRVKEGRPIKYLLPESVENYIYKFKLYI"
     gene            32874..33452
                     /gene="yqeK"
                     /locus_tag="PLG49_02105"
     CDS             32874..33452
                     /gene="yqeK"
                     /locus_tag="PLG49_02105"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOQ16138.1"
                     /translation="MIDYDEMKQKLQSSLSPKRFLHTLGVVETAEELAKIHGVDKEKA
                     IISAYLHDCAKDIPEKMKIRFCKEFHIPLDKWIEKNIELCHSFLGAEIAKREYGIIDE
                     EILNAIRYHTTGRKNMTDLEKLIYLADYIEPNRNYFQGLESIREYARKDLNQAMIFAL
                     TNTIEYVKKNKGILHPLTLEALEDLKSKKGDQ"
     gene            33454..33822
                     /gene="rsfS"
                     /locus_tag="PLG49_02110"
     CDS             33454..33822
                     /gene="rsfS"
                     /locus_tag="PLG49_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOQ16139.1"
                     /translation="MADLKNNSQEILKIAYKILDDKMGEDIKILDIRELSPLADYFII
                     VSGKNINHVKTLADELEDHLYNNGFQMINKEGYASGGWILLDFGLVVFHIFDKEMRNF
                     YNLERLWRDAKLVSIEELNE"
     gene            complement(33863..35218)
                     /locus_tag="PLG49_02115"
     CDS             complement(33863..35218)
                     /locus_tag="PLG49_02115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOQ16140.1"
                     /translation="MFVFRLFSKKYVALFLITIFFFELTLSFKIFAQIEPIIQSDSAI
                     VMDSKTGAILYGKNIHRKQYPASITKIMTALLAIEKGNPDDIITFSDNAVYSIDWGSS
                     HIGMREGEQITLNDALYGMLLMSANEVSNAIAEHIDGSVENFADSMNEKAKELGAYNT
                     HFVNPHGLHDPDHYTTAYDMALITREALKYDKFREVAGTLTYVIPPTNLVNEPRYLAH
                     QHRLYNNKAYPNSYYEGCEGGKTGYTNEARHTLVTFAKREDLELIVVVLKSEKQEMYE
                     DTKRLLDYGFENFEPSILIEAGASAGKVTLGQNEGLKEEKSVEVYAEKTLETVLPKEL
                     TEDIKQEITLSNKLSFPIYEGDVIGSLNYIYKNEIIASVNVLSAENVEGNDSILESLS
                     QNDIKKKSASITDNKVIRLSIMSVLTILILVLFFLTINLINRLRQKRRYWDLFKDYNK
                     R"
     gene            35304..36038
                     /locus_tag="PLG49_02120"
     CDS             35304..36038
                     /locus_tag="PLG49_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ16141.1"
                     /translation="MNKQLLIKGCIIIVFIICGILYINNQFDSIDSAKNQSTFLENDM
                     REDVELFSELSSDNTEEVNTNDKEERADADKERKHTEEKTEEVFLYVYICGAVENPGV
                     YSLREGSRIIDVLNMAGGALEDADLNLLNLAERIVDEQKIYIPKIGEEIDKTQIKVEN
                     RNDNALSIEPKAAGSDTVQNSLININTASSEQLQSLPGIGKTIAQNIIDYRNTNGPFQ
                     SINEIKNVPRIGEKTFEKIKDKITVN"
     gene            36049..36735
                     /locus_tag="PLG49_02125"
     CDS             36049..36735
                     /locus_tag="PLG49_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ16142.1"
                     /translation="MKSKVLVVDDEKLIVKGIKFSLEQEDLEVDAAYDGEEALEMVKK
                     EKYDLIILDVMLPKIDGLNVCRQIREFSNVPIIMLTAKGEDMDKIMGLEYGADDYITK
                     PFNILELKARIKAILRRVTNIQKESNNKRIKISGLEMDISSRRVFIDGKEVNLTAKEF
                     DMLELFASNPGKVYSREALLETIWGYDYPGDVRTVDVHVRRLREKIEKNPSQPDYIHT
                     KWGVGYYFKD"
     gene            36770..38146
                     /locus_tag="PLG49_02130"
     CDS             36770..38146
                     /locus_tag="PLG49_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ16143.1"
                     /translation="MILVNFLPLIIFSMTVYKRLENYFIEERKAELLRQANVTAGHIA
                     IGKYMLDDTKKELFAYEIEQISKELESRIIVVDAQGFEIYDSSVKEQKEEGKKSHVYK
                     EMLIALEGKDLAVKQADNTVDAFASIVDQDQILGVVRISAPIDNLVSETLDTLKNQLY
                     IFTVLISAIIGIISFFASEIITNPLKGMVKVVQKITEGHLDQKVDISGHDELAELGIA
                     FNNMSSKLLQIDQSRQEFVSNVSHELKTPLSSIKVLSESLMLQEDVPVEMYKEFLKDI
                     DSEIDRLTAIINDLLLLVKMDQKEVPMTINSFNLNQLVQDILKRLYPLANKKDIELIY
                     ESFREVLIEADEMKVTLAISNLVENGIKYTPEGGRVKVIVDADHQNAFIKVIDTGIGI
                     PEEDYEKIFQRFYRVDKTRDRETGGTGLGLSITHRTVLLHHGSIKVNSKEGEGSEFIV
                     RLPLKQNS"
     gene            38322..39272
                     /locus_tag="PLG49_02135"
     CDS             38322..39272
                     /locus_tag="PLG49_02135"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOQ16144.1"
                     /translation="MNSKKTVFVSTAILALSLLVVVFAGYYMSKNKEDKHINQLKLYF
                     IDSANSELVEEKREVDLESKEDLIRYAVEQLQKMPNTVGLKPAIPEALKINKIIIEDD
                     NVKLDISSYYNEMSPQEQVLLRAALIKTLTELDFVYSVEFLIDGKPLAGYDGKAIGAI
                     TQEDILLDAKGNDVASTNVQTIKLYFADQNGEKLVLEEREIVVNPNVPLEKYVVEQLI
                     LGPERNDLEMTVPKETVIKDIKTKDGICYVDLSSDFQTKHWGGSAGETLTIYSIVNSL
                     TELPNIKKVQFLIEGEKQATFKGHYDFSIPFERDESLIAD"
     gene            39290..41734
                     /locus_tag="PLG49_02140"
     CDS             39290..41734
                     /locus_tag="PLG49_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOQ16145.1"
                     /translation="MKRPLVWITFFYLFGIIGGRYSLSASYSVLIFFILSSSVLILYK
                     KFTWKGILFFPLVFLTGFININKALNSGSAVKAFVDSHQTRNFKILGTVEDISYNQDG
                     KITCIIYAKKLIKDSNSIEKPIKIKAYINSKQKIEWGDFIEVEGEISEFEPCRNPGGW
                     NEAVYMKSRRIEYKFYGELSRVITSSDSASKLIFLLRERLSNVYECLLAEKEAAIIKA
                     MILGEKAGLGQEEKTLYQQGGISHILAISGLHISIISFSLEKLLKILKMKKIFKNLFI
                     VSFLSIYCVLTGFGVSTIRAVIMISVILLGEVLLREHDFCSSLSFAALLILLWQPLFL
                     WDAGFQLSFTAILGLIFISPLFKRLYFLPKSIRNLLSASLGAGFATLPIVAYHFYYIS
                     TIAFLLNIIVVPLAIFLVAFGFLAGLVGLFWIDGAAFIMGIVHYILSFYEYMLHLFLK
                     IPYAYIITGRFSPWQMIFYYLSLFIPVGYIRLKRKNKLNKKLWIYSFIFTTFVWLFLF
                     IKPDSFHIVFLDVGQGDSIAIHTKSKKHVLIDGGEKDSSKVLLPYLRYKGIECLDAVF
                     LTHPDKDHLGGLVGLLKSIKIKKIYVTVKDIGSDELYRDFIIQANHSGIPCHLLKQGD
                     KINFDELVFYCLYPENNEIYLENGWNRASMTLKMVYKEQSFLFTGDIEKEEEEKMTHQ
                     YKPMDVDFLKVPHHGSKTSSTMEFIEWCNPEYAVISCGKNNRYGHPHEEVLKRYADKN
                     VKVINTADSGAITIYSDGKKFSIHTMLDNRGNEDDNIKRRIEKKYFSVSLLILWGGAL
                     FAQLLFGGNTKKVNSR"
     gene            41685..42596
                     /gene="holA"
                     /locus_tag="PLG49_02145"
     CDS             41685..42596
                     /gene="holA"
                     /locus_tag="PLG49_02145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ16146.1"
                     /translation="MLNYYLGEIQKKLIPGDLEIMNMSLFEGEEITANQVIESAQALP
                     FMNDKRLVIIKNSGFFTAKRKKEAELILQFLDDIPPFICLVFAEENVDKKNKLYKKLS
                     QMKAVVEFQFPKEQDLLNWIEKKFKKYNKEIDRRTSLHLVRTVGRSMIDLENEINKLI
                     AYKEDKINIEDIDKICVKSLEAHIFDLLKAIGYKKTDEALQIYVNLIRNKEAPIKILT
                     MLTRQIRLILQVKYLSEQGYNAKKITETIQEAFFVVNECLKQAQLFSMDQLKQGLKAC
                     LEADLASKTGKMDPGLAVEVLIIEFNK"
     gene            complement(42627..42893)
                     /gene="rpsT"
                     /locus_tag="PLG49_02150"
     CDS             complement(42627..42893)
                     /gene="rpsT"
                     /locus_tag="PLG49_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ16147.1"
                     /translation="MANIKSAKKRIKIIEKRTLRNRIIKSKVKTAIKKVVLAVNEGNL
                     EQAKAAYALAAKEIDKAVSKGVFHKNNGARKKSSLAKLVNSIGA"
BASE COUNT        14685 a         5812 c         8850 g        13685 t
ORIGIN      
        1 acttggccgt gcattgcacc aaagaggtta tttcttttct gtttctgttc cttcaagagt
       61 aagtgatgaa ccatttaatc ctttttccga tcctttccag tatgcgccga taggggcagc
      121 agtggatgaa tttgttgtca tgctatataa tgaacacggc tggcctggca gtggtcctgg
      181 tcctggtcca gtggtatcaa taggctggat ggacagagtc ttaaaatata caatgacaaa
      241 aatgcccagg gataagattg ttgctgctgt atcggttttt ggctttgatt ttaatctgga
      301 tacaggcagg aatacttatg tgacttatca gatggccatt aacagggcac aacaatacaa
      361 tgctcaaatc atttttgatg aagtaaccca gacgccgatg tattcataca cagatgaaca
      421 aggccaacgg catgaagttt ggtttgaaga tgcaaaaagt attcgggcaa aagttcaaaa
      481 agcatgggat ttaggaatta aagggatagc gctttggaga ttgggtatgg aagatccagc
      541 catctggacc atgctcagag atgaaacagt tgtcagaaaa gatattggtc tataaatttt
      601 aagatattat taaaaaagtg tgctattgca cacttttttt aatgatttaa ccattccatt
      661 aaggaaccat aaagattttg aatatcttga taaccctgct catacagagc agctaatttt
      721 tttggatttt tttcgattct tttgactttg acaggagtag aagggcggat aacaaaggct
      781 tttccttcat tttccaactg ttcaatataa tctaatgttt cattgtacat gatatgacgc
      841 ttgtccaggg cttctactag attgggataa tccgcataaa atctttgagc aaatttatga
      901 cttttagaag gcctttttct gtaaccctta tgcctggtta atacaataac attttttgta
      961 ttcccgtctt caatagattt ttttattggt atggcgtctg taattcctcc atccaataaa
     1021 taatgatttc taaattcaac aatatgactg ataaagggaa gactcataga agctttaacg
     1081 gcttcaaaaa agtcttctga attgtcttta taaaaataca tgggctttcc tgaaacacaa
     1141 tcggttgtac agaccacaaa agtttctttt gcctgttgaa aactgtcgta atcaaaggga
     1201 caaagtttat tcggtatgtc atcaaaaaga aattttgtgc caaataattc tttttcccga
     1261 aggaggtttg aaagacttat gtacctttta tcctgaatat aattcagatt aatatctcgg
     1321 tttctccccc tttgcctgga gatatatgaa atcgcattac aagctcctgc ggatacacca
     1381 ataatatagg gaaaaaataa ttttttatca attaagaagt ccaagacccc agcagtatac
     1441 attcctctca atccgccacc ttccagaaca agtccggttt tttccaaagt cttgcctcct
     1501 ttctttggta ataattgtaa tgatacgctt ttcaaatgaa aatgtcaagt ttctgacaaa
     1561 agataatatt tgacagttta aagtaaacgc agtaaattaa taggtaaata aataagctca
     1621 tgcatttgga taaaaggagg tctttttaaa atgacaaggg atgaggcata tgaattatta
     1681 aagaagtata cggagtcaga aagcctgatt aagcatgctt tagccgtaga agctgccatg
     1741 agagggtatg caaaaaaatt taatgaagat gtagagtttt ggggaataac gggtttaatt
     1801 catgatctgg attatgaatt atatccagac aagcatcctc ttgaaggggt caagatttta
     1861 gaggaaaatg gttacccggt cgaattaatt gaagcagtaa aaggccatgc agaccatacc
     1921 aatacgccaa gacagaccct tttggctaaa accctttatg cggttgatga gctttccagt
     1981 tttattattg catatatatt agtcagaccg aataagagtt ttgaagacat tcaattaaaa
     2041 tccattaaga aaaaacttaa ggataaagcc ttcgccagag gtgtggacag agatatcatt
     2101 aaaaaaggtg ccgaggaatt aggagtagac ttggatgaac atattatgac tgttgcagat
     2161 gctttagtag aaagagaaaa agaactcaat caaatgggag taagtctggt ataaatattc
     2221 atgcactaat tgaaatttaa tgaatattta aaggaatgat attgcataaa aaaacaaaca
     2281 ggtgtataat tatgaaatca ttatttaatt ttttagggga gatattatga gtaatatgga
     2341 atggatcaaa cgtggtaata aagttattat gaatacttat aagcgttttc cccttgcctt
     2401 aacagaaggt gaaggtgttt accttaagga tattgagggg aaaaagtatt tggattttgc
     2461 ttcaggcatc gctgtaaatt ctttgggaca tggcaataag accctgatca aagctttaga
     2521 agaacaaatg aataaaatca tgcattgttc aaatttatat tggaacctgc ctgctataga
     2581 agctgcagaa atgcttatcc aaaacagcat ttttgataaa gtgttttttt gtaacagtgg
     2641 ggctgaagcc atcgaagcag ctttaaagct ggcaagaaaa tttggtaaaa agagacatgg
     2701 ggaacattgc tatgaaatta ttacgatgaa aaattctttt catggcagga cttttggtgc
     2761 cattacagca acggggcaga caaaatatca aaaagattta aatcccttga tggatggaat
     2821 cttatatgca gattataatg attttgagtc ggttaaaaag ttagtatctg ataagacatg
     2881 tgctattttg ttagagccta ttcagggaga aggcggtata agaccagctg ataaagattt
     2941 cttattgaaa ttaagagact tgtgtaatga aaaagaaatt cttctgatgt atgacgaagt
     3001 tcagtgcggt attggtcgta caggttattt atttgcttac gaggcttatg gtgtcgcacc
     3061 agatgtgatt tcccttgcta aaggcttagg aggcggtttc cctatagggg ctatgatggc
     3121 tgttgaaaag tattctgatc tttttggacc cggggatcat gcttcaactt ttggaggaaa
     3181 cccagctgca tgttctgctg ccaaagtggt tctgactgca ttaatccagg aaggtatttt
     3241 ggaaaatgta aaagcacagg gagcatatct gagagaaaga ctgaatatct taaaagaaaa
     3301 atatactatt attacagatg tcagaggcat gggtttaatg cagggtatag aattaagtct
     3361 tcctgtggct aatattgttg aactgtgtat acaaaaaggt ctgcttctcg ttggtgcggg
     3421 agaaaaagtc attcgatttg ttccaccttt aattgttggg aaaaatgaaa tagatgaagc
     3481 cataaatatc cttgaaacgg ttttaaaaga agtttaaata agattttcaa ccggtctgag
     3541 aatgataata attttagggg gttgcttatg ggattaaatt taagaggaag aagttttttg
     3601 actttaaaag attttacgcc tgctgaaata gattatttac tggacttagc ggaaaactta
     3661 aaaagaaaga aaaaaatggg aatacaggga gacttgttaa gaggtaaaaa tattgcactg
     3721 ctttttgaaa aaccttcaac cagaacccgt tgtgctttta ctgttgcatg tattgatgaa
     3781 ggtgcgcatc ctgagtattt aggtaaagag gatattcagt tgggaagcaa agaatcggtt
     3841 gaagatacgg caagagtatt aggccggctt tttgacggaa tccagtttcg gggctttaag
     3901 caggaaacgg tagaaacctt ggcaaagtat agcggtgtac ctgtttggaa cggattaaca
     3961 gataaatatc atcctacgca agttcttgct gattttttaa caataaaaga aaagtttggt
     4021 tgcttaaaag gaatccgatt ggtatatata ggtgatggac gaaacaatat ggcaaacagc
     4081 ttaatgattg gggcctccaa aatgggaatg gattttgtta tttgtgcacc gaaaagtctc
     4141 tggcctgaag atatcttagt tgaagaatgc aaagcttttg caaaagaatc cggggcgaag
     4201 gttacaatta cagatgatat agatagtgct gtgtttggag cagatgtcat ttataccgat
     4261 gtatggtgct ctatgggcga agaagcaaaa gcagaagaaa gaattaaatt attaaaagcg
     4321 tatcaggtga atgcagattt gatgaaaaag acaggaaaag aagaaactat ttttatgcat
     4381 tgtcttcctg cagttaaagg gaatgaagtg acagaagaag tgtttgaaag taaagcatct
     4441 gtggtatttg acgaagcaga aaaccgtatg cataccatta aagcggtgat ggttgcgact
     4501 ttatcataaa atttttctga ttcattgatt aattcaataa atataattaa attcattgac
     4561 tagagaatta tttttagcta taatttttac aaacattgtt atttatatgt caattgattt
     4621 tattattttt attttagggg gattaaaatg gatagagtgt ataatttttc tgctggacct
     4681 tcaatgctgc ctttggaagt tttacaaaag gctcaaaaag aaatgttaaa ttatgaaaac
     4741 agtggtatga gtgttatgga aatgagccac cgttctaaag tttttgatga aataattcaa
     4801 ggtgcagaag aagttttaag ggatatcatg aatattcctc ataattataa ggtgcttttc
     4861 ttacaaggcg gggcttctac acaatttgca atgatacctt taaatctttt tagaaatagt
     4921 aaaaaagcag attatgtcaa taccggacaa tggtcaaaga aagcgatttc tgaagctaaa
     4981 aaatatggta cagtaaatgt cgttgcttct tcagaagaca ctacctttaa ctatattcct
     5041 gatttagata aaagcacttt ttcaccggat gcagattatt tccatattac atccaacaat
     5101 actatttacg gaacccgtta tgtagaattg ccggatacag gagatgttcc attggtatct
     5161 gatatgtctt catgcatttt atctgagcct tgtgatgtaa caaaatttgg cgtaatttat
     5221 gctggagcac aaaagaatat tggtccggcg ggtcttacag tggttattat ccgggaagac
     5281 ttaattggtc atgctatgga tattacacca acaatgctga attataagac tcatgcagac
     5341 aataattctt tatataatac accacctact tacggaattt atattgcaaa attagttttt
     5401 gaatggatta aagaaaaagg cggcttagcc gaaatggaaa aaatcaacaa agaaaaagca
     5461 aatattctat atgatttctt agataattcc agtttctata aaggaactgt tgttccaaaa
     5521 tatcgttccc taatgaacat accttttgtg actccttcta aagagttaga tgaaaaattt
     5581 gtaaaagaag caacagccaa tggtttagta aatttaaaag gtcacagaac ggttggcggc
     5641 atgcgtgcaa gcatttataa tgctatgccc attgaaggag taaaagcttt agttgaattt
     5701 atgaagaaat ttgagatgga aaatcgttag ggggattaga atgtacaaaa ttaagacatt
     5761 aaataagatt tctgaagtag gacttcaaca ttttgataga aataaatatg aaatatcaga
     5821 ggattgtcca aatccagacg gaattatagt tcgaagtgca aaaatgcatg atatggaatt
     5881 gcctgcttca ttaaaggcca ttgccagagc aggggccggg gtaaataaca ttcctgtaga
     5941 tttatgctca gaaaaaggaa ttgttgtatt caatacccct ggagcaaatg ccaatgccgt
     6001 aaaagaattg gtaatcgctg gtttgttatt atcttcaaga aaagttgtcg atgcgattag
     6061 ctgggctaag agtttaaaag gtaaagggga agaagttccc aaacttgttg aaaaaggaaa
     6121 atcagctttt gcgggtcctg aaataaaagg caaaaaaata ggtgttattg gtttaggggc
     6181 cattggtgtt ttagtagcta atgcagcaaa atctcttggt atggaggttt atggatacga
     6241 cccttatatt tctgttgatg cagcctgggg cttatcaaga gcggttcata aagcggatag
     6301 ccttgaagaa attcttgctg attctgacta tattaccatt catgttcctt tacttgagga
     6361 tacaaaaaat atgatgaata aagaaagatt tgccttaatg aaaaaaggtg caagactgct
     6421 taatttctca agagctgaat tagttaatac ggctgattta cttgaggcaa taaaagaagg
     6481 tattatagct tgttatgtta cagatttccc aactgacgaa ttattagatg tagaaaatgt
     6541 aattgctatt cctcatttag gtgcatccac accagaatct gaagacaact gtgctgttat
     6601 ggctgtgcaa caaatgatgg attatcttga aaacggaaat attaaaaact cagtaaattt
     6661 ccctgattgt gaaatgacca gaacaaccga ttaccgtaca gttgtggctc ataaaaatat
     6721 acctggtatg gttggtaaaa ttacgacgac aatagcgaat gacggagtca atatcatgga
     6781 tatgatgaac aagagtaaaa aagaatgtgc ctataccatt attgattccg acaagcctgt
     6841 atcggatgag gttgttgaaa aactcatcca ttcagatggt attcttacag ttaggacttt
     6901 aaacagaatc aaatagcaac taagactcag acaacagatt tttgcattgc ttaaatctgt
     6961 tgttttttta agacataaat attgtaaata attatattat aaattatgga atagggtgag
     7021 gtgttatttt attatgaata atacaaagat tggatttttc actaagttaa caagaaccat
     7081 atttgatttt aatatttatc ctcatgttat tggggaaagc acaggaagag ccgttgcttt
     7141 tttacttttg ttttctttac ttttaggaat aatcggttct ttaattactt cagccaggta
     7201 tcttctttcg ggtggtttaa cagttatcac tgatttaatg cctgattttg aatatgataa
     7261 tgggattcta accgtagaag atatggaaga gccttttatt tatgaaatgg attccaataa
     7321 aatatttgtt atcgatatga atgatgaagt ggatgcggat attttagaat attattctga
     7381 aggaatcatg cttaaacaag atagagtgat tgcagttcaa tatggaaata ttcaggaagt
     7441 atcttatacg gaatttttgg attcttataa tatatatcac tttagcaagg aagatttatc
     7501 aaaaatcctc ccagtcattg atattggtat tgttgtgata atgattggtt ttggagttat
     7561 tggaagcgtt attggtaaaa tgatcagttc tctttttgtc agtgtattgg gattaatcgt
     7621 aaatgcagtt tcagaagcta aattaactta tagcgatatc tataaaacag gggtttatgc
     7681 tttgattctt ccttctttaa taaagctaat tctttcatcc gcaggtctta atgttggctt
     7741 tataagaatg atattttttg ttgtctatta tggtattgtc attttctatc ttgtaaaggc
     7801 tattacaacg ataaaaagaa ataagcaaga aatgatgtat cagcaatatc agcaaaatat
     7861 gatgcaataa aaaaagaaac ttaaaaacat ggggacagtt ccttgtttca ccggcaatct
     7921 ggtgaaacaa ggaatcgtcc ccatgtttct taaagtattg gttcaacaga agcaggatct
     7981 gaattcatat tgttgagtac ttctttaaga attaaaatgc cttcgtgaat ctgtttttca
     8041 ttgacggctg caaaactaag gcggaagtag ttaagggatt ctttagataa agaaaataat
     8101 gaacctggtg aaataatgac atttcttgat aaaagtatgt cagacagttt atctacagag
     8161 atagaagctt ttaatttaat ccataggctt aagcctccgt caggaggaat gtaactgatc
     8221 tcatttttta aatgttcatc aagtaatttc attaaataaa tatatctttt cttatatatc
     8281 ttacgaattt cattaatatg gcttttccag agccctttcc gaagatataa gtcaaatacc
     8341 cgttgtataa aacctgatgt agtaatgtca gtggtttgtt ttgcagataa aatattgtct
     8401 aataagtttt ttggtaccac taagaagccc aggcggagtc ctggcattaa tatttttgaa
     8461 aagcttttta aaaagatcac ccggtttcga tgatccaggg cttttactgg tatgcttttc
     8521 tcttcagaat aatttaaatc acttaaataa tcatcttcaa taatataggt attatatttt
     8581 tcagccaaat ctaatatagt ccttttcttt tttaatgaat atgaataacc tgtaggattt
     8641 tgataatagg acataagata tataaatttt ggatggtata ttttcaggta gttttcaaga
     8701 atttctaaat ccatgccatc tgtttctaag ggaacctcaa tgattcgtgc gccccggctt
     8761 tggaatgcgg caatggcacc atgataagtt ggcttttcta ccaataaagt atctccatat
     8821 tgcagcaggg ccttggaaag aatatcaatt ccttgttgtg ctcctgaaat aatttgaatt
     8881 ctatcttttg agctatttat cccataagat ttaagatagt cagcaacaga ttccctgagt
     8941 ttttcatatc cttggctttc ctgatagcta aaagcattac ctttatcttt atccagaatt
     9001 tcattgacaa tgcacttaaa actttcaaca gggaacaaat ccgtacttgg ggatatactg
     9061 gcaaaattga tgctgtcttc tgtaatcatt atttgtttta tctctttttt ttcttcataa
     9121 gcaggagaag gcatatcttt tacaggaata ggcgatacat aggtgccact tcctacttga
     9181 gagtaaacaa gccccttttt ttccagatat ttataggcat ttatgacagt tacattgttg
     9241 acttttaatt tattggcaag tttacggatg gtaggcagtt ttgtattggc ttttaagata
     9301 tctttagaaa ttaaatcata aagagcatca gctagttgct gataaagagg aattttacta
     9361 tttctatcca attgtatcga tacaaagtaa tccataatga aaaatcctcc ttaatatatt
     9421 gaaaacagct agtatatttg tataataatg catatataaa aattcatttt tttctttatc
     9481 ctgaaaaatt gtatcgatac aattttatca ttgttttaga cagctgtcca gacgttatca
     9541 aaatctttca tgaaactttt tgattaagga ggtttatgaa tatgagtaat aaatatgata
     9601 taacagaaag tttaaaaggt ggggtgatta tggatgtcac caatgcaaaa gaagcaaaaa
     9661 ttgcccagga agcaggagca gtagctgtta tggcattaga acgggttcct gcggatatca
     9721 gaagagaagg aggcatagca aggatgtcag atcctaaact catcaaagaa atcaaagaag
     9781 ctgtcagtat tcctgttatg gcaaaatgca gaataggtca ttttgtagaa gcacagatat
     9841 tagaggcttt ggacattgat tatattgatg aaagtgaagt tttaacacct gctgatgata
     9901 aataccatat tgataaaaaa cttttcaggg ctccttttgt atgtggcgcc agggatttag
     9961 gagaggccct gagaaggatt ggagaagggg cgtctatgat ccggacaaaa ggagaggctg
    10021 ggacaggaga tattgtccag gcagtaaagc atatgaggac aatgatggct cagattagac
    10081 agttgcaaaa tatggcaaag gaagaaagaa tggcctttgc aaaagaaatt gctgcgcctg
    10141 ttcatttggt tgaagaagtt gctgaattag gaagacttcc tgtagctaat tttgctgccg
    10201 gaggcgtagc aactcctgcc gatgctgctc ttatgatgca attaggcagt gatggagttt
    10261 ttgtaggttc agggatattt aaatccggaa atcctgcaaa aagagccaaa gcgattgtag
    10321 aagcggttaa atattataat gatccaaaaa aattagccga gatatcggaa gatttgggag
    10381 aagagatgaa ctagtaaaaa agcgatagga agtaggaaga agttaattcc atgcttccta
    10441 tttttaattg catctttctt gcatttttta tttctaattt ttaacttttt atttccagtt
    10501 tctaacttct ttcttgcttt atttttaaat cagtgtaaaa ttcattgtag gacaatttta
    10561 aagaggagtg gaagtaatga aagaactatc actgcacatt ttagacatta ctgaaaatag
    10621 tattagggcc aaagcgacag aaatatatat agaagtagaa gaagacctta ataacaatct
    10681 tttttcattt gtaataaagg ataatggaaa aggaatggat gaagaatttt taaaggcagt
    10741 aaaagatcct tttacaacgt ccaggactac gagaaaagtt ggtctgggaa ttcccctttt
    10801 agaagcagcc tgtaaaagat gtaatggcgg tcttgaaatt attagtgtag ttaatcaggg
    10861 aacacagatt aaggcatgga tggattataa ccatatagac cgggcacctt taggggatat
    10921 ggtcagtacc atatccaatt taataatatc caatccggat atcgattttg tttattgcca
    10981 taagtataat gaaaaatcat ttaaaattga tacgagagaa attaagaata tccttgatgg
    11041 tgtgccgatt aacgatcttt ccgttataaa ctggctgaaa aattacataa aagatgaatt
    11101 gacagaaatt aaacaaaaat aatttaaaaa aaagtaaatt attacttttt aaaagagaaa
    11161 atgattaaat aaaatagtat attgcataca agatacgcga aaacacagca gaatttttta
    11221 attaaaatgg taaataaatc taggtgaatt gtgcattttt tatcatttct tgatgaattt
    11281 actgctttgt attatgatat ataatatatt atgatattta cttgaaataa ctaaatctgt
    11341 ttttttgcat aattttattc aatgaatagg gggatttata tgcagttgct tacttttaaa
    11401 agaggcattc accctcctga tggaaaacat ttaactgaaa ataaaccaat agaatatctt
    11461 cttcctaaag gagatttggt atatcctatg gttcaacaca taggagcacc atgtgagcct
    11521 atcgtaaaaa agggagacag ggtcttagtt ggtcaaaaaa ttggagaaac taaggcttat
    11581 gtttcatcac ccatacattc aagtgtatcg ggtacggtaa aaaatattgc acccatgctt
    11641 catcccaatg gaaataaggt tttatctgtc atagttgaaa atgatggttt atatgaagaa
    11701 catgaatcta ttggaccaaa aggggattat aaagatcttt ccagagagca gattattgaa
    11761 attataaaag aagctggtat tgttggtatg ggtggggcag gattccctac ttttatcaaa
    11821 ctgtctcctc ctccggagaa aaagatcgat acgattattg taaacggggc ggaatgtgaa
    11881 ccttatttaa cttctgacta tagagtgatg ttagaagaaa cggacagagt ggttactggc
    11941 ttgaaaatta ttttaaaact gtttcctgag gctaagggct atattgctgt agaaaataat
    12001 aaaatgaaag cggttgaagc tcttctggaa gagacaaaaa atgtagataa cattgaagta
    12061 aaggttctaa aaaccaagta tccccaggga tcagaaaaac aattaattta tgccttaaca
    12121 ggcagagaag taccttctgg aggattgcct gcggatgtgg gttgtattgt gcaaaacatt
    12181 gatacggttg tagccatcca tagggctatt gttagaggaa ggcctttaat gagaagaata
    12241 gtaacagtta ccgggggagc aattaaagat ccaaagaatt tcaaagtaaa gatcggaaca
    12301 tcttacaggg aactgataga agccgctgga ggctttgtag aagaaccagt gaaaattata
    12361 tctggtggac cgatgatggg aattgcgctt cctagtttgg atgtgccagt atgtaaggga
    12421 acttcagcca ttttatgttt aaccagagaa gaagcacagt taccccagga acaaaattgt
    12481 atccgctgtg gcagatgtat ttctgcctgc cctatgggtt taatgccttt ggaacttaat
    12541 cgatatgcca ttcataaaga catagaacaa tttgaaacaa tgcacggaat ggattgcatg
    12601 gaatgcggca gttgttctta tatatgtccg gcaaaaagac atattgttca gtccatcaga
    12661 acggcaaaaa aagcagtatt agagaacagg aagaaaaatg cgtaaagggg gagcttaatg
    12721 gctaacggaa agtatacagt atcctcatcg cctcatatac gttcaacgga taccattcaa
    12781 agtattatgc aagatgtttt cattgcttta cttcctgcag cgtttgcagg ggtttggttt
    12841 tttagatttt cagctttatg gattattttg atatccatag tgacatgtgt tggagcagag
    12901 gccttatggc aaaaactaac aaatcaaaaa attacaataa acgattttag tgcggcagta
    12961 acgggtttac ttcttgccct taatttgcct ccgaccgttc ctttttggat tcccatagtg
    13021 ggaggagttt ttgcaattat tgttgttaag cagttttttg gaggcctggg acaaaacttt
    13081 atgaatccgg ctttggccgc cagggttttt ttactcactt catggactcc tcaaatgacc
    13141 agttggactt ttgacggtat gacaacggca acacctttgg gaatattaaa agaaggcgga
    13201 gcacaattac cttctctggc ggatttattg ataggacgca ttggcggttg tattggagaa
    13261 acatctgcca ttgccttgtt gattggagca gcatatttgc tttataaaaa agtgatatca
    13321 ataagaattc ctgcaacttt tataggaacc gtttttgtcc taacctggtt acttggaaga
    13381 ggcggtatgt tttattccaa tgctttgtat gaaattttta caggaggact tattttagga
    13441 gcatttttta tggcgacaga ttatgcttct tctcctgtga cagaaaaagg tcagtatatt
    13501 atgggcttag gttgtgggat tattactgct gtcatccgtt tatacggagg cctgccggaa
    13561 ggggtatcct atgccattct tattatgaat ctggcagttc cattaattga aagatataca
    13621 agaccccact attttgggga ggtgaagcaa tgaaggaaat tttaaaactt ggcctgatat
    13681 tatttataat aacagctgtt tctggcggat tattgggttt tgcctatgaa attactaaag
    13741 aacccatagc agaacaggtt aggctggcca atgaagaagc tatggtaaaa tcttttcctc
    13801 aaggtgaaaa atttaaaaaa atagaagcag agataccgga agattctaat gttgtagaaa
    13861 ttcatgccgt atataagggg aatgttttag agggttttgt agtaaaagtt ttatcgaaag
    13921 gttttggtgg ccctatagag atgactgttg gcgtatcaaa agacggaatg gttcagggcg
    13981 ttagtatact tggtcacagt gaaacgcctg gtttgggggc taaggctcaa gaggatgatt
    14041 tcctttctcg ttatcagggg aaatctggca tattgacggt tgtgaaaaca ggagccagta
    14101 aggataatga aatagaagct atttcaggtg ctactattac gtcgaaggca gtgacggatg
    14161 gaataaatga agtattagac tttattgaaa atcatctgga tgcggataat ttttaaggtt
    14221 tttttgattt taattaaagg aggattacat tgaagacaat agcagaaaga ataaaaaacg
    14281 gggttttggt tgaaaatcca acctttgttc aggtaatagg aatgtgtcct accttagctg
    14341 taacgacagc agcaaaaaat ggaataggta tgggtttagc aacaacggcg gttttgattg
    14401 ggtctaatgt atttatttct cttttaagaa aattcattcc atcaaagatc cgcattcctg
    14461 cttatattgt gattatagcg agttttgtga ctcttgtaga atttctgatg cagggatttg
    14521 tacccgcttt atataaacaa ttaggtttat ttattccttt aattgttgtg aactgtatta
    14581 ttcttgcaag ggctgaagcc tatgcttcaa aaaatgacat tttaacttca ctttttgatg
    14641 gtttagggat gggacttgga tttaccctgg cattaacagt aattggttct gtcagggaaa
    14701 ttttaggtgc aggccaatgg tttggtattc aaatcatgcc ttcctcctat caaccagcga
    14761 ttatcatgat tttagctccc ggtgcattct ttacgttagg aattttaatg gcgattctaa
    14821 attacatcag gaatagaaat acgaaaaaag cataggggga gttacaatgg aaatatttat
    14881 tattttttta agtgccattt tagttaataa ttttgtttta tcaaggtttt tagggatttg
    14941 tccttttctt ggggtttcta aaaaagtaga aaccgccata ggaatgggtg ttgcagtaac
    15001 ctttgtaatg actttagctt catttattac ttatattgtc tatcattgga tgctggtacc
    15061 acttagaatt acctatttat acaccattgc ttttatcttg gttattgcag ctttagtaca
    15121 atttgttgaa atggttattc aaaagacaag tccaaccctt tatcaggctt tgggtgtgta
    15181 tcttcctctt attacaacca actgtgctgt attaggggtt gctgtaatca atataaatga
    15241 aggttataat ttaattaaga gtgtgattaa tggttttggt gcagctttag gttttaccct
    15301 ggcaattgtt ttatttgccg gaatcagaga aagaatggag cataataaca ttttaaatgc
    15361 atttaaaggt ttcccgattg cattgatcac ggcaggttta atgtccattg ctttcttagg
    15421 attccaggga ttgctttaga gaggagggga aactgtggat ataacaagca ttctttaccc
    15481 ggttatcagt ataggcggtt taggtgtatt atttggagca ggtttaggtt atgcctcaaa
    15541 gaaatttgct gtagaagtag atgaaaggat tcctcaagtg agggatctgc ttcctggggc
    15601 aaactgtggt ggttgtggtt atgcgggttg tgatgctttt gcaaaggccg ttgtagaaga
    15661 aggtgtagat atttgtactt gttcagtaaa cagtccggaa gcgatagaaa agattgctgc
    15721 ccttttaggt gttgcggcag gttgcagtat aaaaaaagtt gctcaggtaa aatgtaaagg
    15781 gacttgcagt aacagtaaaa ataaatacga atattatggt attatggact gtcaggacgc
    15841 tgcagctacg cccggtggtg gtgcaaaggc ctgtagttat ggctgtttag gccttggaac
    15901 ttgtaaaaag gtttgtatgt ttgatacgat aagaatagaa gatggcattg cggtgattga
    15961 tgaagagaaa tgtacgggtt gtggaaattg cgtagaagcg tgtcctaaag cagtgattga
    16021 attggttcct caaattaatc aagtgagggt tgtatgtaat tcaaaggata aaggaaaaga
    16081 tgtaaaagag gcctgttcag taggttgtat tggctgtagc ctttgtgtaa agacttgtga
    16141 atacaatgcc attgatttta aagataatct tgctcatatt aagtacgatc aatgtactca
    16201 atgtatggct tgtgttcaaa aatgtccgac taaagtcata gctggccagc ttgaattagc
    16261 ggaactataa ccgtaaaagg tttccggtgc ttattctttt gagataagca ccatttttta
    16321 ttgttttaat tcctacgggc ttgtgctaaa ataggttaat aaataatttt taggagtgat
    16381 ttgccttgaa tataaaaaag ggaaagaaca actgggtatt atttattttt atattaatgg
    16441 gtattgttat tggaggtttt attggacatt atttaggaca atttccatat tttcagtggc
    16501 ttaattttgg aaaagaattt ggctttcaga aaccagttgc attagatctg caaataatat
    16561 atatcgaaat tgctttaaaa atagaaataa caatttcaag tatattaggt gttgttgcgg
    16621 ctatacttat atatagaaaa atatagcaat caatggtaca agtttataaa gcaggtggtg
    16681 tagtttttat ggagaaaatt attttagcat ccgcttcacc aagaagaaag atgcttttaa
    16741 atcaaatagg agtttctttc gaggttatgg tgagtgatgt agaggaaaaa attaattatg
    16801 aacttcctcc agacgaactg gttaaagaat tatccaggca aaaggcgatg aatgtagcca
    16861 aaagattggg agaaggtttt ttagtgattg gtgctgatac ggttgtggtc tttgatggaa
    16921 aaattcttgg aaaacctaaa gataaagaag atgcttttaa aatgcttttc atgcttcagg
    16981 gacattggca tgaagtatat acaggggtga ctattattaa gacaaaagat atgttagtgg
    17041 aatcagactg tgaaaaaacg gaagtttata tgagaaaact atcttcttcc ctaattaatc
    17101 agtatattaa cacaggagaa cctttggata aagcaggtgc atatggcata caagaaaaag
    17161 gcggactttt tattgataaa ataaacggag attacaataa tgtcgttggt ctgcctgtaa
    17221 aattaattgt ggctttagca gaaaaaatgg gggtggattt ataccaaaat atttttaaaa
    17281 tcaggagttg agaaaagtga ctgtttcaaa tgaggtacat atgtctatga aggatttacc
    17341 gaagttggaa agaccttatg aaaagttaga gtattttggt ccacaagctt tatccgaagc
    17401 tgaattattg gccattattt tcaggactgg atcccgagag ttaagggcgg tggagttggc
    17461 acagagatta ctggctttag aaaaagagga agggatttat ggcttgtacc aatatagtct
    17521 ggatgaatta atgaaggtga aaggaattgg cagggtcaaa gctattcaag ttaaagcggt
    17581 tttggaatta tcaaagagga tttcaaaaca tcaggccagt ttaaaatacc ggattaattc
    17641 cccgggaagt attgctttaa tttatatgga agaaatgcgc tatttaaaac aagagcattt
    17701 taaaattata ttattggata caaaaaacca gataataggt tgtcaggaag tcagcaaagg
    17761 aagtatcaat tcttccatag tccatccaag agaagtattt aaatatgcta taaagaaaag
    17821 tgcagtaagt attatcatgt tgcataatca tcccagtggg gatccagctc ccagccagga
    17881 agacattaag gttactgaaa ggatgaaaga agcaggagaa ctgatcggca ttcatgtgct
    17941 cgatcatatt attattgggg acggggttta tgtgagttta aaagaaagag gcattatata
    18001 gactgaaaaa atactaaagc ttaaggactt aagcaattat tgtttatgtc ctttttaagt
    18061 tttcgatatt ttttgttgaa aaaaataata aaaagggtta tgatgatact agcataggga
    18121 aagatgttaa tgataatatc attgtcatta attttgtata taaattgtag ctagactaaa
    18181 taatattagt ataatttttt taatacactc ttattgtggc attaattttt ggatgactag
    18241 gaggaaaagt tatgtttttt tcaagagatt taggtataga tttaggaaca gctaatacgc
    18301 tggtatttgt taaaggtaaa ggaattattg ttaatgaacc ttcagtggtc gctgaaaata
    18361 aaacaacaaa agagatttta gctgttggtg atgaagcaaa acaaatgata ggaagaacac
    18421 ctggtacaat tgttgccaca aggcctttaa aagatggtgt tattgcagat tttgctacaa
    18481 ctgaagctat gttgaaatat ttcataggga aagcttataa aaaagggctt tttactggca
    18541 agccaagagt aattatttgt gttccttcag gggttacggc agtagaaatg cgggccgttt
    18601 atgatgctgc gagaaatgct ggtgcaaaag aagcccacct gattgaagaa cctatggcag
    18661 cagccattgg tgcagggctc cccgttggag atcctacagg aagtatggtt gtggatattg
    18721 gaggaggtac cagtgaagta gccattattt ccttaggggg aatcgtaacc agtaaatctc
    18781 tgagggtagc cggggatgag atagacgaat atattgttaa ttatataaag aaagagtaca
    18841 atcttatgat tggagagcgt acggcagaag acattaaaat gaccattggt gctgcttttc
    18901 caaaagaaag agaggaaagt aaagaaataa gaggcaggga tttggttaca ggacttccca
    18961 aaactctgaa gataacttcc actgaaatta tggaagcgat tagagatcca attaatgcaa
    19021 ttgttgatgc aataaaatat actttagaaa aaacccctcc tgaactggcg gcagacatta
    19081 tggaatcagg cattatgcta acaggtggag gtgctctgct ttcaggattt gataaaaaga
    19141 tttcccagga gacaggcatg cctgtacgga ttgctgaaga gcctttaaat tgcgtcgctt
    19201 taggaacagg tcttgcctta gaacaaattg agaccttaag aaatatcttg atatctccta
    19261 aaaaaattta gtaaatttat tgataggaga gagagttcgt gagacgctca tttaaaatta
    19321 aagaaaaata tgtgattgta attgttacaa ttgttttatt aattacaatc attttgactg
    19381 ccggtaaaag gtataatatt acctttattg aaaaaggcct gggttatatt gttatgcctg
    19441 ttcaaaagac ttttagcagt gtgggggaat ggatcagcga tcagatatat tttatcaaaa
    19501 atattaaaga acttgaaaag caaaatactg agctggctaa aaaggtggat gaattgtctt
    19561 atgaaaataa gattttgcag cagtataaag atgaaaatac ccgtttacga aaactattgg
    19621 aattagataa aaaataccca gattatccta aaataggcgc tgaaataatt gggaaagatc
    19681 ctggaaactg gtataatgta tttttaattg ataaaggcag caatgatggc ttagaagttg
    19741 atatggttgt tctttcagga aatggccttg tagggcatat tatagaagtt gctcccaatt
    19801 attctaaggt attatccatc attgatgacc ggaatgcagt gagttcaaaa gtattaagaa
    19861 caggggattt gggtattgtt aaaggagatt tgaccttatt aaatgaaggt ttgtgtaaaa
    19921 tggaatatgt agatgctgag gcagatatta ttgttggaga tgaaattgtg acttctaatt
    19981 taagtgatat ttatccgcca ggcatcatga ttggtgttgt taaagaaatt gaaacagaaa
    20041 gccatggact caccaagtat gcgcttattg aacctgtagt agattttcgg caccttgaag
    20101 aagtcttagt cattaatatg aaatggaaca aaaaagatga tgcaacaaag gagtaattta
    20161 attgcgtcgg atattagcca taacagtcat tttattgctt actaatattt tacaggggac
    20221 atattttcaa ggccttgaaa taagaggtat tgtccctaat ttctatatta ttattatcgt
    20281 atcttttgct ttactaaggg gaagtaaaga aggtgctatt gttggttttt ttgcaggtct
    20341 gttacaagat atttattatg gaacatccat tggattttac gcccttttag gtgtttacat
    20401 agggtatttt tcaggcaaat taaacaaaag tttttatagg gaaagttttt ttcttcctct
    20461 tattttaacg attgtcagta gcttttttta tgatatggcc atttacatat ttacttattt
    20521 aataagagga aggattcaga tatcttatta tttttataat atcattcttc cgggtatggt
    20581 ttatacaggc gtcttttctt tatttgttta tcaaggcatt tattatatca atgaaaaatt
    20641 agaaatccgg gaaaaaaagt acaggaattt tttcaaataa gtccaaagtc tattaaaggc
    20701 ttaaaggtgg ggaaaatatg atcaatgata attctgttat ctttaaagga accaaaaatg
    20761 gattaacaat cttattaagc cctgatatag attttaatat tttaaaaaat catttagaaa
    20821 aaaaaataga agatgctaaa gattttttta aagatgctaa aaccagcatt atgtttaaag
    20881 gcagagattt aacggaagaa gaagaatatc agcttctgca aattattacg aagaagacgg
    20941 agctgaacat tagttatatt tttgatgaag aaaccagtat agatatgcct gaaaagatcg
    21001 aaaaatcatt aaggtctgtt caattaaatg atgaaagaga gcaaattgac aaaacaaagt
    21061 attataaagg gaccatccgc tccggacaat gtctggaatt tgatggcagc gtagtggtca
    21121 ttggtgacgt taatccagga ggagaaattt atgcagaagg caatattatc attcttggtt
    21181 cattaaaagg cattgttcat gctgggtgta aaggttatga taaagccttt attgctgctt
    21241 tgatcatgaa gcctatgcag cttaggatat gcaatattat tacaagatct cctgataatc
    21301 cggattatag tactcatgca aaggtatttc ctcaggttgc ctatttatcg gatgggaata
    21361 tatatatcga accaattgat tataaattat tagaaaattt gtgactttta taattatatg
    21421 gttttttttg taaaaataaa atgggtgcat tttacttcat tatctgttaa aataatatta
    21481 ttagatttag attatgtttt gccaacaagg gggaaataac atgagtcagg ttattgttgt
    21541 tacttcaggt aaggggggcg ttggaaaaac tacgacaacg gctaatattg gaaccggact
    21601 tgcattaaaa ggtcacaaag ttgtattact ggatgcagat attggactta gaaatttaga
    21661 tgttgtgatg ggattagaaa atcggattgt ttatgattta attgatgttt tagaggggca
    21721 ttgcagaatt aagcaagcac ttattaagga caagcgttat tcaggattat atttgcttcc
    21781 tgcagcccaa acccgggaca aaacggcagt aaatcctgag caaatgcaaa aattgtgcag
    21841 tcaattgaaa gaagaatatg attatgttat tattgattgt cctgcaggta ttgaacaggg
    21901 ctttaaaaat gccatagctg gtgcggataa ggctattgtt gttacaacac ctgaagtatc
    21961 tgccgtaaga gatgcagacc gtattattgg ccttttagaa gcaagtgaat taagaaatcc
    22021 aacccttatt ttaaatcgta ttcgtatgaa tatggttaaa cggggcgaca tgatggccat
    22081 ggaagatgtt gtagaaattt tagcagttga tttactaggt gttgttcctg atgatgagaa
    22141 tattgttatc tcaacaaacc gtggagagcc tgcagttact aatccagact ctttagcagg
    22201 acaagccttt agaaatattg ttgaacgcct tttaggcaac aatgttcctc ttttagattt
    22261 ggatataaat gaaggtttta tggataagat taaaaaatta tttggaatag gtaattaaag
    22321 gatgtgaatt accatggatt tattcagttt ttttagccgg aaaccaagct caaaggatgt
    22381 tgcaaaggac agactaaaat tattacttat tcatgacaga attaattgtt ctcccgacct
    22441 tttagaaatg atgaaaacgg acattattaa ggtaatttcc aattatatgg acattgatga
    22501 agatgagttg gatatacaaa ttggtcaaac gaaggcagaa ggttcagatg gtacggttcc
    22561 agtattgtat gcaaatattc ccattaaaaa tgtgcgcaaa gtgggtaatc aataaaattt
    22621 taagcaggaa tatattcctg cttaattcat tatgaaaggc gatcagtatg atttcgaata
    22681 aatacttaaa atcatttgat atagtccttg tgtttttagt gactgcatta gtaactttgg
    22741 gcattatagc aatcagcagt gcacaacatg ttaattcagg tgcagatcct atatatgtca
    22801 aaagacaaat cctgtttttt gttgtgggac ttatatttat gcttattact gcatttatag
    22861 actaccattt tttaggagat ttatatattg ttgcttatat tgcaggaatt cttctgttga
    22921 tctctgttct gttatttggc gatgccagta aaggcgctgt aaggtggctg gatttaggtt
    22981 ttactagaat tcagccgtct gaatttgtaa aaatcattat tatcttattt ttttcaaagc
    23041 ttattgataa aagacagcaa caaattaata aattttctac tctattgttg cttggtatct
    23101 tattggtaat tcctgtactt ttaattatga agcagccaga tttatcaacc agtttggtat
    23161 ttgtttttat atttttaatg ttattatatg ttggtggaat aagttataaa tatattatta
    23221 gtgcgattct tattggcatt cccctgatat ttctgatttt gatttatatt caaaatccca
    23281 atcaaaaact attaaaagat tatcagaggg atagaattat ggctatgatt cataaagata
    23341 ataaagaata tgccgatgat tatttccaaa cggaacaatc cgttcatgcc ataggctcag
    23401 gtcaacttta tggtaagggt ttatataagg gaactctaaa tcagttaagt tatttgcctg
    23461 aaccacatac cgattttatt ttttcagtaa ttggtgaaga atttggttat ataggatgta
    23521 tttcgattat tgtcttgttc tttttgttaa ttgtacgaag cttatggata gcaaaagatg
    23581 caaaagacct ttttggaaag ttattaattg taggtgtggt ttctatgata ggctttcagg
    23641 cctttgtgaa tattggggtt gttacagcac ttctccccaa tacgggttta cctctgccat
    23701 ttattagtgc aggaggaagt tccttatggg caaacatgat tggaatagga ttaatcttaa
    23761 atgttagtat gagaagaaaa aaatcattct attaggaggg aattttatga atattggttt
    23821 agttgcccat gatgcaaaga agaagcttat ggaaaactta gtcattgctt accgtcacat
    23881 attgtctaag cataatcttt atgcaacagg caccacagga agattagtgg aggaggcagc
    23941 caatgtaacc attcataaat atcttgctgg tcatttaggg ggagaacagc aattggcaac
    24001 tcaaattgct cataatcaaa ttgatatggt tatttttctg agggatcctg tatcaagaaa
    24061 acttcatgag ccagatgaag aaaaaattat gagattgtgt gatattcata atattccggt
    24121 tgcaacaaat ctggctacag cagaacttct gattaaagcc ctggagcgcg gagatttagg
    24181 atggagagat attgttaaat aaatatttca taaaagcccc tgctttgtaa tataatttat
    24241 tgtatcctat tttggattaa ttctaatcca cagaaaagat aaaatagcat aggaggggct
    24301 ttatggagaa aaaaagaaat atctatagtt accatggacg aagaaacagt aggaatcgta
    24361 aaaacaacag taaagatatt tttttaaatt ttataatgaa acaatttgta gtcagtactt
    24421 ttttgttttt attaattatt atacttaatt tgctgccttt aaatattacg atgaacctga
    24481 tgcagcagat taatgaaaga atttattatt caatgacctg ggatgaagca gttgaaacag
    24541 tgaaatatac agcttcacgg atacctaaag taaaaaactg gttagaagct acggatgaaa
    24601 cggaacaaca gaggaatagt aaaagccagg aaagtattga caataacaat gacatgcttt
    24661 tggaggattc agacatagat actgaaaata agatggatag tacagatcat cttgaagatg
    24721 tttttgacag taatgaagat atatttataa tagaaccaga cttgggggag tctcaggagg
    24781 gaataattcc ataaatttaa ggagggaatt aagaagctat aatgaagagt ggattaatcc
    24841 cgtggatgga attattacct ctttttttgg tgaaaggatc aatcctgttt tagataaaaa
    24901 agaatttcat gatggcattg atattgcggc gcctaaagat gagcctattg tggcagtaaa
    24961 aagcggcata gtggtaaagg ttggtgtttc aaatacttat ggaaattttg ttgaatataa
    25021 agtcgaaggt ggttatataa taaagtatgc ccattgtagt gagatatttg taaaagaagg
    25081 tgattatctg gaacaaggtc aaattattgc ttcggtaggt aatacgggct tggcaacagg
    25141 ttatcatctt cattatggta tatcaaaaaa aggggattat atagatccaa tagcgtatgt
    25201 ggaactgcca gttttggaaa ctttattgga ataatcaaat gaatcatttg attatttttt
    25261 ttatgtaatg tatcatataa aagatacatt taaaatgata caagcgcaaa attactggag
    25321 gaaaatttat gacaaagtta agaatagctg ataaaatttt aaaacaggtc gaaaaacctg
    25381 caagatatat tggacaggaa gtgaatagtg ttattaaaga taaaaataaa gtggatatcc
    25441 gctttgcttt ttgttttccc gatgtgtatg aggttggtat gtctcattta ggaatgcaga
    25501 ttttatatta ttttttaaat aaacgtgaag atacctattg tgagagggtt tttgcaccat
    25561 ggacagacat ggaagaaaaa atgagagaaa atcatattca tttatttgct ttagagtctc
    25621 aggatgaaat caaaaatttt gattttattg gttttaccct gcaatatgaa atgtcttata
    25681 cgaatattat taatttacta gatttagcag gagtttctcc atggagtaaa gatagaaaag
    25741 aagatgatcc tattgtttgt gccggaggtc cttgtgctta taatccagaa ccattggcag
    25801 attttattga ctttttttat attggggaag gagaagcttc actaaatcaa gttttggata
    25861 tttataaaga acataaaaag cagggaggta cgaaagaggc ctttttagag aaactattgg
    25921 atatagaagg aatttatgtg cctcgttttt atgaagtaaa atacaaagac aatggcctta
    25981 ttgaaagctt tacacctata cacaaaaaag ctaaagcaaa aataaaaaaa caacttgtaa
    26041 aggatttaga tgactcatac tatccttcaa aagttatctt accatggatt caaacggttc
    26101 acgacagagt taatttagaa cttttcagag gttgtatccg tggctgccga ttttgccatg
    26161 caggttttgt ttaccgtcct gtaagagaaa aatcaaaaga gattttgatt gaacaaggaa
    26221 aaaaactatt aaaaaataca ggttatgaag aaatatcctt atcttcccta agtaccagtg
    26281 attacagtgc attcaaagaa ttttcagaag aattactgga aacttgtcaa aaggctgggg
    26341 tcaatatttc tttaccttct cttagaattg ataaattttc aattgattta atgaataaaa
    26401 ttcaaactgt aagaaagagc agcttaactt ttgcacctga agcaggcagt cagagactca
    26461 ggaatgtgat taataaaaga atcactgaag aagatatttt aaagggctcc cagttcgcct
    26521 ttgaaggtgg atgggatcgg attaaattat attttatgat tgggttgcca acagaaaaag
    26581 aggaagacat tttggaaatc aaagaacttg cttataaaat tcttgatact ttttatagta
    26641 ttccaaagga aaagagagca agaaatatta agattgttgt cagttcttcc tgttttgttc
    26701 cgaaaccttt tacacctttt caatgggaac cacagtgtcg ttatgaagaa tttatggaca
    26761 ggcagaactt attaaagaaa agcataaaga gaaaagaaat ccagtataat taccatgatg
    26821 ctactataag ttctttagaa ggcttattat cccgggggga caggagagtt ggacaagtca
    26881 tttattatgc ctggaaatat ggcgcaaaat ttgacagttg gacagaaaag ttcaatatgg
    26941 ctgcatggaa aaaagctttt gaactgacca atattgatcc tgctttttat gcaaacagaa
    27001 aaagagccta tgatgaaatt ttgccctggg atcatatttc cataggagtt agtaaggctt
    27061 ttttaataaa agaacatcaa aaggccttag atgaaaaaac aacgcctaat tgcagggaaa
    27121 aatgtgcagg ttgtggggct aagatatttg aaaggggcgt atgccatgaa attcagaatt
    27181 aaattttcaa aggaggggca tttgaaattt atcgggcatt tagatttgct ccgtttaatg
    27241 caaaggtgta ttaaaaaagc agaacttccg gttgcctatt caaaaggttt taatcctcat
    27301 cagctcatat atatagccct gcccctttct ctaggtgcga ccagtgaagg ggaatatatg
    27361 gatatagaat taacagagga ctctcttatg acggagacag agattaaaga caagttaaat
    27421 gcggtttttc cagcaggatt aaaagtagaa aaggtaagcc gcgtgaaaga taaggcaaaa
    27481 tcaggtatgg cggctgttga tggggcagaa tataaggttt ttatatctaa agaaaaaatg
    27541 cctgtttatt ttaatgaaaa aacagaggct ttttttgagc aaaaagagat ttggattgaa
    27601 aaaacaggta agaaagagac taagaaaatt aatataaaag ataagatata ccgttattgt
    27661 gttcaggaag ataaagattt ctggattatt catttattat tagcagcggg cagtaaagat
    27721 aacttaaaac ttgaaacact gatagaagct ctttatcaat atattgcttt agattataat
    27781 ggctatgata tagatattca cagagtcgac atttattctg aagtcgacaa tcaattgatt
    27841 cccctgtcaa atctataaaa gggaagtgaa tatagtgttc acggatatta ttgtagatgt
    27901 aggcattcat caaatccggg tcggtgttgt agaaaatcag aagctggttg aattgtatat
    27961 tgaggaagat aataaagaaa aaaccgtggg taatatttac cgggggattg taaaaagaat
    28021 attgcctggg atacaagcag cttttattga tattggaaaa aataaaaatg cttatttaaa
    28081 tttaagggaa aatcaatttt tgggagaaaa cggtcaacaa ttaaaacaag gggatcatat
    28141 tactgttcag gttcaaaagg aagctatggg aagcaaaggt tataaagtaa ccagtcattt
    28201 tagctttgca ggaaagtatt gtgtccttat tcctggagaa aaatcgattg gaatttcaag
    28261 aaaaataaat gatgaaaagg aaagacttcg cttaaaagag ctttttaaca gtttaaattt
    28321 agataattac ggaattatct tcagaaccaa tagtctgggg aaagaggaag aagaactgat
    28381 aaaagaaatt caatatttaa aaaaccagtg cgaagtgata aagcagaaag aaaggtatgt
    28441 taaggcacct gccttactgt ataaagagtt gtcttttcct tttattgcgg caagagattt
    28501 aatgactaaa gaaataagac attttattat taatgataag gaaatgtatg aagaaatact
    28561 tgcgtttctg gatctaatgg atgccagttt aaaagaaaag ctggtctata aagaagaaaa
    28621 gaatttattt gcttattttt tactggaaac ccagattgaa agggctttaa gccgccatat
    28681 ctggctgaaa agtggtggca tgattattat tgaccacact gaagccctga cagttattga
    28741 tgtgaatacg gcaaaatata ctggaaaaaa agatatggaa aaaaccattc ttcaaaccaa
    28801 tatagaagct gcccaggaaa ttgccaagca gcttaggctc agaaatatag gtggtatcat
    28861 tatcatagat tttatagata tgaaagaaga agcacataaa gagatgctga taaaaatact
    28921 tgaggaagag ttaaaaaaag acagagtaaa aactagtatt tttggcttta caaaattagg
    28981 cctggtggaa ttaacaagaa aaaagacagg accttcttta acctctttac ttcttggcaa
    29041 atgtccgtat tgttcaggta ggggcatggt ggcttcggta aaatttatag cagataaaat
    29101 agcaaaagaa attgattata ttttcacaga aaccatttat aatcatatta ccattgaagc
    29161 gaacaaagat attattcatt ggttttgcag tggagattat tcctatcaac aggcttttga
    29221 agaaagatat gagaaaaaac ttgaatttat tgaaaataat caattagcta ttgaccagta
    29281 taagctcatt aaaaaaagct aaaaatggaa caggaagatt tttgagctat cttgacaaat
    29341 atataatgtt gtgttatcat ctaacggtgt gtagccgcac gaagaggttt aaacttcatt
    29401 tgatgaagta ccgtattcgg cgagtttggg tttaaggagg tgtacgtatg tacgcaattt
    29461 ttgaaacagg tggaaaacag tataaagtac aagaaggaga cgttgttatt gtagaaaagc
    29521 ttggacttgc tgcaggagaa aaagtaactt ttgataaagt tcttgcagtg tctaaagatg
    29581 ataagctttc tgtaggaact ccttatgtaa acaatgcgtc tgttgccgct tctgtagtag
    29641 aagaaggtaa aggaaagaaa gtgattgttt acaagtacaa acctaaaaaa ggttatcaca
    29701 aaaaacgtgg tcatagacaa ccttacacaa aaatcaaaat tgaaagcatt aattgttaat
    29761 tatgttgaaa gtcgtttttt atttaaataa aaacaagcaa ttatgcggat ttgaaattag
    29821 tggtcatgcg ggatatggaa aatacggctc tgatattgtc tgtgcggccg ttagtacttt
    29881 agctattaat acggtaaatt ccatcgaggt cttttcttca gatcctattt gtatagaaac
    29941 agataaaaaa gatggactaa tgatatttag tctggatgaa aaggcaaaag cttatcctgc
    30001 ggacgagacc ctgttatttt taaaatccct ggaattaggt ttgaagaata ttcaaaaaaa
    30061 ctataaaaag cacatcttaa tttcctataa ggaggtgtaa ttatgttaag aatggacctt
    30121 cagtttttcg ctagtaaaaa aggagtagga tcttctaaaa acggtcggga ttctatatct
    30181 aagagattag gtgcaaaacg tgcagacgga caatttgtga aagcaggaaa cattttatat
    30241 agacaaagag gaactaaaat tcatcctggt attaatgtag gtagaggtaa agatgatact
    30301 ttatttgctt tgattgacgg tcgtgttaaa tttgaaagaa aaggtagaga taaaaaacaa
    30361 gtttctgttt atcctgcata agaatttatt gagtgacgaa aggcttcaaa tcagttcatt
    30421 tgaggccttt tttatttata agatatagca ggaattgtat tattcgtata taatattgtt
    30481 gttattggtc agttagatga tgaatttgtt aatgattgta gtaataatgg gagatactaa
    30541 agagaatact aattatagtt taattaagaa ggtgaagcaa agatgtttgt cgatcgtgta
    30601 aaaatttata tagaatctgg cagtggcggg aatggatctg tatcttttcg ccgggaaaaa
    30661 tatgtaccca atggaggacc tgatggaggt gacggaggaa aaggtggaga catcattttt
    30721 gaagtggatg aaggtctgaa taccttaatg gatttccgcc atcagaaaca tttcaaagca
    30781 gaaagaggag aagatggtaa gaaaaagaag tgtaatggca aaaatggtaa agatttaatt
    30841 attaaagttc ctccagggac catcataaag gaagcagaaa ctggaaagat tatggcagat
    30901 atgactgggg aaaccaagtc aaaggtttta ttaaaaggcg gaaaaggcgg caggggtaat
    30961 cagcattttg ccacagcaag cagacaggcg ccgagatatg cagaaagagg acaggaaggt
    31021 aaaggttatt gggttatttt agagcttaag gttattgccg atgtgggttt agttggcttc
    31081 cctaatgctg gtaaatcaac gcttttatcg gttttgacca atgcagagcc caaaattgcc
    31141 gattatcctt ttacaacttt acatcctaat ctgggggttg tcagaaaccc ttatggaaga
    31201 gattttgtta ttgcagatat tccgggaatt attgaagggg ctcaccaggg gataggctta
    31261 ggccatgatt ttttgagaca tattgaaaga acaaaaattt tgctccatat ggtagatgtt
    31321 gcatcaatag aaggccggaa tcctgcagaa gatgttgaaa aaattaattt tgagctaaaa
    31381 gcttataatg aagagttgtt aaaacgacct caaattattg tagcaaataa aattgatgtg
    31441 atggaaaatg aggacaatct gaatttattg aaggagactt atgaaacaaa aggtattaaa
    31501 gtaattccaa tttcagcagc tactcaaaaa ggcttaaaag atttgttaat tgaaattagc
    31561 aaaatattag atcaagtaga tgaagaacct gtaaaatttg aagaagactt tgattacttt
    31621 gaagagcctg aagaaataaa agaacccttt acgattaaga aaatatcttc ttcttactat
    31681 gttgtagaag gaacaggtgt ggaaaaagtc atcggttata ccaatttcga atcagaaaaa
    31741 ggctttgctt tcttccaaaa atatctcaga gataagggaa ttatcgatgc cttagaaaaa
    31801 gcaggaattc aggaaggcga tacggtaaaa atatatgact tagaatttga gtattataaa
    31861 taatgatttt aaggagccag gtgatacaga tgattacaag taaacaaaga gcatatttaa
    31921 ggagtttagc caataccata gagcctattt tccaggttgg taaaagtggt ataacacctg
    31981 atttaacaca aggaattcat gaagctctag aaaaaagaga gttggtaaaa attaatgtct
    32041 taaataattg tattatgtca gccaaagaag tggctgttat ccttcaggaa aggaccaatt
    32101 cagaaattgt tcaagtgatt ggtaataaaa ttgttttatt caggcaatct aaaaagaagc
    32161 cccggatttt tctgtctgaa taatatggaa taacaaaatg agagggtatt tttatgagga
    32221 atgtatttaa tgggttagaa catccggaat ccatagccat tatgggaggg acttttgatc
    32281 ccatacatta tggtcatttg gttacagcag aggcagtacg acataaatac aatatcagtc
    32341 aggttatttt tatgcctacg ggacagcctc cccataaaaa ggaccaggaa attagtcata
    32401 gtgaacacag gtatttaatg agtgttttag ctacagctac caatccctat tttaatgttt
    32461 ccagaatgga aattgaccgg aagggaacca cttatacgat tgataccatt aagagtttaa
    32521 gagaacttta tggtgatgag acggaacttt attttataac gggtgctgat gcccttcatt
    32581 atatttttac ctggaaagat accgaggaac tgctcaaatt atgtactttt gtggcagtaa
    32641 cccggccagg ttataataaa gaaaaattaa ttgaagccat tgaaatcata aaaaataatt
    32701 atgaatccaa aattcatttt ttggaagttc cggccctttc tatatcttct acagatatca
    32761 ggaagcgcgt aaaagaaggt agacccataa aatatttact gcctgaatct gtagaaaact
    32821 acatatataa atttaaacta tacatataaa ttatggttta aggaaggacg cttatgatag
    32881 attatgatga aatgaagcaa aaactccaat cttctctttc tcctaaaaga tttttacata
    32941 ctttaggcgt agttgaaacg gctgaagaat tagcaaagat tcatggcgtt gacaaggaga
    33001 aagctattat atcagcttat ctccatgatt gtgccaaaga tatacctgaa aaaatgaaaa
    33061 ttcgtttttg caaagaattt catattcctt tggataaatg gattgaaaaa aacattgaat
    33121 tgtgtcatag ttttcttgga gctgaaattg caaaacgaga atatggtatc atagatgaag
    33181 aaatattaaa tgcaattcgt tatcatacga ccggaagaaa aaatatgaca gacctggaaa
    33241 aactcattta tttagcagat tatatagaac caaaccggaa ttattttcaa ggtttagaga
    33301 gcatacgaga atatgcaagg aaagatttga atcaagccat gatttttgcg ttgacgaata
    33361 ctatagaata tgttaagaaa aacaaaggaa ttcttcatcc tctaacttta gaagctttag
    33421 aagatttaaa atctaagaaa ggagatcaat aagttggcag atttgaaaaa taattcgcaa
    33481 gagattttaa aaatagctta taaaatttta gatgataaaa tgggagaaga tattaaaata
    33541 ttagatatcc gtgaattatc tcccttagca gattatttta taattgtttc cggtaaaaat
    33601 attaaccatg ttaaaacctt agcagatgaa ttagaagacc atttgtataa taatggattt
    33661 caaatgatta ataaagaagg ctatgcttct ggtggttgga tattactgga tttcggactg
    33721 gtggtgttcc acatatttga taaagaaatg agaaattttt ataatttaga acgtttatgg
    33781 agagatgcaa aacttgtctc catcgaagag ttaaatgaat aatgattaaa aaatgggcaa
    33841 aaacttttgc ccatttttat ttttaacgct tgttataatc tttaaataaa tcccagtatc
    33901 ttcttttttg cctaagccgg ttaattaaat tgatagtaag aaagaaaagg acaagaatga
    33961 ggatggttaa tacggacata atgcttaaac gaataacttt attatctgta atggaagcac
    34021 tttttttctt aatatcattt tgggacagac tttctaatat actgtcattt ccttcaacat
    34081 tttcagcaga aagaacattt acagaggcaa tgatttcgtt tttatagatg taattcagac
    34141 taccaatgac atctccttca taaatgggaa aagataattt attggaaaga gtaatttctt
    34201 gtttaatatc ttctgttaat tcttttggaa ggaccgtttc caatgtcttt tcagcataca
    34261 cttctacgct tttttcttct tttaaacctt cattttgacc cagggtaact tttccggcag
    34321 aagcacctgc ttcaattaat atacttggtt caaaattttc aaaaccataa tctaacaagc
    34381 gtttggtgtc ttcatacatt tcttgttttt ctgatttaag tacaacaacg ataagctcca
    34441 ggtcttccct ttttgcaaaa gtgactaagg tatgccttgc ttcattggta tatccggttt
    34501 ttccaccttc acaaccctca taataggaat ttggataagc tttattattg taaagtctgt
    34561 gctggtgtgc cagataacgg ggttcattaa ccaggtttgt aggaggaata acatatgtaa
    34621 gggttccggc aacttccctg aatttatcat atttgagcgc ttctctggta ataagggcca
    34681 tatcgtatgc agttgtgtaa tggtcagggt catgtaaacc atggggatta acaaagtgtg
    34741 tattataggc tcctaactct ttggcttttt cattcatact gtctgcaaaa ttttcaacag
    34801 aaccatcgat atgttctgct atggcattgg aaacttcatt agcagacata agaagcattc
    34861 catataaggc atcgtttaag gtaatttgtt ctccttctct cattcctata tgactgcttc
    34921 cccagtcaat gctgtaaacc gcattgtcag aaaaagtaat gatatcgtca ggatttcctt
    34981 tttctatggc taaaagggca gtcataattt ttgttatact ggctggatat tgttttctat
    35041 gtatattttt accataaagg attgcaccag ttttagaatc cataacaatt gcagaatctg
    35101 attgtataat tggttcaatt tgggcaaaga ttttaaaaga caaagtaagt tcaaaaaaga
    35161 aaatggttat aagaaacaga gcaacatatt ttttgctaaa aagacgaaag acaaacatta
    35221 tctagctcct ctctatagtg tcaaaaagcg gtctttttta atatgaattc ccttaataaa
    35281 tatgaatgga aggatgatga tggatgaata agcaattatt gattaaggga tgcattataa
    35341 tagtatttat tatatgtgga atattataca tcaataatca atttgattct atagatagtg
    35401 cgaaaaatca gagcactttt ttagaaaatg atatgcggga ggatgttgaa ttgttttctg
    35461 agctttcttc tgataataca gaagaggtga atactaatga taaagaggaa agggctgatg
    35521 ctgataaaga aaggaagcat acagaagaaa agacggaaga agttttttta tacgtataca
    35581 tatgtggtgc agtagagaat ccaggggttt attcattaag agaaggcagc aggattattg
    35641 atgttctgaa tatggcaggg ggggctttgg aagatgcaga tttaaattta ctgaatctgg
    35701 ctgaaaggat tgtcgatgaa caaaagattt atattcctaa aatcggagaa gaaattgaca
    35761 aaacacaaat aaaagtagaa aatagaaatg ataatgcttt atcaattgag ccgaaagctg
    35821 ccggatccga tacagttcaa aatagcttaa tcaatataaa tactgcttcc tctgagcaac
    35881 tgcagagttt acctggcatt ggaaaaacga ttgcacaaaa tattattgac tatcgaaata
    35941 caaatggccc ctttcaatcc atcaatgaaa taaaaaatgt tcccagaatt ggggaaaaaa
    36001 cttttgaaaa aataaaagat aaaattactg taaattagga ggttagctat gaaaagcaag
    36061 gttcttgtgg tggatgatga gaaattaatt gttaaaggta ttaagtttag tttggaacag
    36121 gaagatttag aagtggatgc tgcctatgat ggggaagaag ctttggaaat ggtaaaaaag
    36181 gagaaatatg atttaattat attagatgtg atgcttccta aaattgatgg tttaaatgtc
    36241 tgcagacaaa tccgggagtt ttctaatgtg ccgattatca tgcttaccgc taagggtgaa
    36301 gatatggata aaatcatggg gcttgaatat ggtgcagatg attatattac aaaacctttt
    36361 aatattttgg agcttaaagc caggattaag gcaatactgc gaagagtgac caatatccaa
    36421 aaagaaagta ataataaaag gattaaaatc agtggcttgg aaatggatat tagcagcaga
    36481 agagtattta ttgatggaaa agaagtaaat ctgacggcaa aagagtttga tatgctagaa
    36541 ttatttgctt ctaatccagg aaaagtttat agtagagaag ctctgttaga gactatatgg
    36601 ggttatgatt atccagggga tgtcagaacg gtggatgtac acgtcaggcg tttaagagaa
    36661 aaaatagaaa agaatcccag tcagccagat tatatacata caaaatgggg ggtaggttat
    36721 tatttcaaag attaggttta aaagcttacg atggaaaata ttagtaagca tgattttggt
    36781 gaattttctt cctttaatta ttttttcgat gacggtctac aaacggctcg aaaattattt
    36841 tattgaagaa agaaaagctg agttgctaag acaggcaaat gtgactgccg gtcatatagc
    36901 catagggaag tacatgctgg atgatacaaa aaaagaactg tttgcttatg aaatagagca
    36961 gatcagtaaa gaacttgaat ccagaattat tgtcgtcgat gctcagggtt tcgaaattta
    37021 tgattccagt gtaaaagaac aaaaagaaga gggaaaaaag agtcatgttt ataaagaaat
    37081 gcttatagca ctggaaggaa aggatttggc ggtaaaacag gcagataata cggttgatgc
    37141 ctttgcttct attgtggatc aggaccaaat tttgggtgtt gtaagaattt cagccccaat
    37201 agataatttg gtgagtgaga ctttagatac tttaaaaaac cagttgtata tttttacggt
    37261 tttaattagt gcaataatag gaattataag tttttttgct tcagaaatta ttacaaatcc
    37321 tttaaaaggg atggtaaaag ttgttcaaaa aatcacagag gggcatttag atcaaaaagt
    37381 ggatatcagt ggtcatgatg aattggcaga gttgggaatt gcgtttaata atatgtcaag
    37441 taaattatta caaattgatc aatccagaca ggaatttgtt tctaatgttt ctcatgaatt
    37501 aaaaacacct ctgagttcaa taaaagtttt gagtgaatct ttaatgctgc aggaagatgt
    37561 tcctgtagaa atgtataaag aatttttaaa agatattgat tcagaaattg acagactgac
    37621 ggccattatt aatgatttat tacttttggt taaaatggat caaaaagaag tacctatgac
    37681 gataaatagt ttcaatttga atcagctggt tcaggacata ctaaaacgcc tctatccttt
    37741 ggcgaataaa aaagatattg aacttattta tgaaagtttt cgtgaagtgc ttattgaggc
    37801 agatgaaatg aaagtaacac ttgctatttc taatttggtt gaaaacggaa tcaaatatac
    37861 acctgagggg ggcagggtaa aagttattgt tgatgcagac catcaaaatg cttttataaa
    37921 agtaattgat acaggtattg gcattcctga agaggattat gaaaagattt ttcaacgttt
    37981 ctacagggta gataagacaa gagacaggga aacaggagga acaggattag ggctgtcaat
    38041 tactcacaga acggttttac tgcaccatgg aagtataaaa gtcaatagta aggaaggaga
    38101 aggctcagag tttattgttc ggctaccttt aaaacaaaat tcatagtttt gtaataacta
    38161 tctgttatga ttataataga agttctaatt tgattagata gaatacttct gattgattta
    38221 gatagccgta aaaaactcaa aaattttgaa gcttacaagg tggaaacttg taaagttatt
    38281 tcatctaaaa caggggcttc aaatgaggaa tgggggataa aatgaactct aaaaaaacag
    38341 tattcgtttc gacagcaata ttggctttat cgcttttagt tgttgtattt gcaggatact
    38401 atatgagtaa aaacaaagaa gataaacata taaaccaatt aaagttatat tttattgaca
    38461 gcgctaattc tgaactggtg gaagaaaaaa gagaagttga tttggaaagt aaagaggact
    38521 tgattagata tgctgtagag caattacaaa aaatgcccaa tactgttgga ttaaaacctg
    38581 caattccaga agctttaaag ataaataaaa ttattataga agacgacaat gtaaaacttg
    38641 atatttcttc ttactataat gaaatgtcac cacaagaaca ggttctgtta agagcagctt
    38701 taatcaaaac tttaacagag ttagattttg tttattcagt agagtttttg attgatggaa
    38761 aaccccttgc aggttatgat gggaaagcga ttggtgctat tacacaggaa gatattcttt
    38821 tagatgctaa gggcaacgat gtggcttcaa caaatgttca aacgataaag ctatattttg
    38881 ctgatcaaaa tggagaaaaa ttagttttgg aagaaagaga aattgttgtt aatccgaatg
    38941 ttcctttaga aaaatatgtc gtggaacagc ttattttagg acctgaaaga aatgatttag
    39001 agatgacggt tcctaaagaa acagttataa aagatattaa aacaaaagat ggtatttgtt
    39061 atgttgactt aagcagtgat tttcagacaa aacattgggg aggttcagca ggagaaaccc
    39121 tgacgattta ttccattgtc aattccctga cagaattacc caatataaag aaagttcaat
    39181 ttttaattga aggggaaaaa caagcaacct ttaagggaca ttatgatttt agtattcctt
    39241 ttgagagaga tgagagttta attgcggact aaatctttgg aggttaatca tgaagcgtcc
    39301 actggtttgg attacatttt tttatctgtt tggtattata gggggacggt atagcttatc
    39361 agcaagctat tccgtcctta tttttttcat tttatcttcc agtgtattga ttttatataa
    39421 aaaatttaca tggaagggga tattattttt ccctttagtt tttttaacag gctttataaa
    39481 tattaacaaa gccctgaact caggaagtgc tgttaaggct ttcgttgaca gtcatcagac
    39541 caggaatttt aaaattttag gaacggtaga agatatcagc tataatcagg atggaaaaat
    39601 aacttgtatt atttatgcaa aaaaactgat aaaggatagc aattctattg aaaagccaat
    39661 aaaaataaag gcctatatta attctaaaca aaaaattgaa tggggcgatt ttattgaggt
    39721 cgaaggagaa atatcagaat ttgagccctg cagaaatcca gggggctgga atgaagccgt
    39781 atatatgaaa agtaggagaa ttgaatacaa attttatgga gaactgtcgc gggtgattac
    39841 aagttctgat tctgccagta agctaatatt tttgctaaga gaaaggcttt caaatgttta
    39901 tgaatgtttg ctggctgaaa aagaagcagc tataataaaa gcaatgattc ttggagaaaa
    39961 agcaggttta ggacaggagg aaaaaacgct ttaccaacag ggaggtattt cacatatttt
    40021 ggctatatcg ggcttgcata tctcgataat atctttttct ttagaaaagt tactgaagat
    40081 cttgaaaatg aaaaaaatct ttaaaaattt atttattgtt tccttcttat caatttactg
    40141 tgtattgacg ggctttggag tttctactat aagagctgtt attatgattt cagtgatttt
    40201 actgggtgaa gttttattaa gagagcatga tttttgcagt tctttaagtt ttgcagcttt
    40261 gttaattctt ttatggcagc cacttttttt atgggatgca ggttttcaat tgtcatttac
    40321 agcaattctg ggtctcattt ttatcagtcc tctttttaaa agattatatt ttttacctaa
    40381 aagtatccgt aatttattaa gtgcttcttt aggggcaggc tttgcaactt tgcctattgt
    40441 tgcctatcat ttttactaca tttcaaccat tgctttttta ttaaatatca ttgtggtacc
    40501 tcttgcgatt ttccttgtgg cttttggatt tttagccgga ctggtgggac ttttttggat
    40561 agatggcgca gcttttatca tggggattgt tcattatatt cttagttttt atgaatacat
    40621 gctccatcta ttcttaaaga ttccttatgc atacattatc acagggagat tttcgccttg
    40681 gcagatgatt ttttactatt taagcttatt tattcctgta ggctatattc gtttaaaaag
    40741 aaagaataaa ttaaacaaaa agttatggat ctatagcttt atttttacta catttgtatg
    40801 gctttttctt tttataaagc cagattcttt tcatattgtt tttttggatg tgggtcaggg
    40861 tgattccata gccattcata caaaaagtaa gaaacatgta ttgattgacg ggggagaaaa
    40921 agattccagt aaggtattgc tgccctattt gcgttacaag gggatagaat gtttagatgc
    40981 agtatttttg acccatccgg ataaagatca tttaggaggt cttgtagggt tattaaaatc
    41041 cataaaaatt aaaaaaatct atgtcactgt aaaagacata ggaagtgatg aactttaccg
    41101 ggattttatt atccaggcaa atcattcagg aatcccttgt catttattaa agcaaggtga
    41161 taaaataaat tttgatgaat tggtttttta ttgcttatat cctgagaata atgaaatata
    41221 tttggaaaat ggctggaata gggcttctat gacccttaaa atggtttata aagagcaaag
    41281 ttttttgttt acaggggata ttgaaaaaga ggaagaagaa aaaatgactc atcagtataa
    41341 gccaatggat gtggattttt taaaagttcc ccatcatggt tcgaagactt cttcaaccat
    41401 ggaatttata gaatggtgta atcctgaata tgctgtcatt agttgtggta aaaataaccg
    41461 ttatggtcat ccccatgaag aggttttaaa aagatatgca gataaaaatg tcaaggttat
    41521 taatacggca gacagtggag ctattacgat atattctgat ggaaaaaaat ttagtattca
    41581 tacaatgcta gataataggg gtaatgaaga tgataacatt aaaagaagaa ttgaaaaaaa
    41641 atatttttcg gtcagtttac ttattttatg gggaggagcg ttatttgctc aattattatt
    41701 tgggggaaat acaaaaaaag ttaattccag gtgatttgga aataatgaat atgtctcttt
    41761 ttgaagggga agagattacg gctaatcaag tgatagaaag tgcgcaggcg cttcctttta
    41821 tgaatgataa aagattagtt attataaaaa attctggttt ttttactgca aaaagaaaaa
    41881 aagaagcaga gctaatactt caatttttag atgacattcc accttttatt tgtcttgttt
    41941 ttgcagaaga aaatgttgat aaaaagaata aattatataa aaagttaagt caaatgaaag
    42001 ctgttgtaga atttcaattt ccaaaagagc aggatttgtt aaactggatt gagaaaaaat
    42061 ttaaaaaata caacaaagaa attgatagaa ggacttctct tcacttagtc agaaccgtcg
    42121 ggagatcaat gatagatctg gaaaatgaaa tcaataaatt aatagcctat aaggaagata
    42181 agattaatat tgaagatatc gacaagattt gtgtaaaatc tttagaagct catatctttg
    42241 atttgcttaa ggcaattggt tataaaaaaa cagatgaagc cttgcagatt tatgtaaatc
    42301 ttattcgtaa taaagaagca cctattaaaa tacttaccat gctgacaagg caaattcgtt
    42361 tgattttaca ggtgaaatat ctgtctgagc aaggctataa tgcgaaaaaa atcacagaaa
    42421 ccatacaaga agcctttttt gttgtcaatg aatgtttaaa acaagctcaa ctttttagca
    42481 tggatcaatt aaaacaagga cttaaagctt gtttagaggc ggatctcgcc tcaaagaccg
    42541 ggaagatgga tcccggtttg gctgttgaag ttttaattat tgaatttaat aaataaaaaa
    42601 taaaccgtcc ctgtggacgg tttttattat gcaccgatac tattaacaag ttttgctaag
    42661 ctggattttt tacgtgcacc attattttta tggaaaacac ccttagatac tgctttgtca
    42721 atttctttag cagcaagggc ataagcagct tttgcctgtt ctaaattacc ttcattcact
    42781 gccaatacaa ctttttttat agctgtttta acttttgatt taataatacg gtttcttaag
    42841 gttcgttttt ctatgatttt aattctcttt tttgctgatt taatatttgc catcttgtca
    42901 cctcctcata ttctaaccat gtggaatgag acactttcag tatggggaat acctacttat
    42961 agtttagttc agtcaaacac attatattct aatctaaaat aataaataag tcaatgaata
    43021 taagaaaaat aa
//
LOCUS       DAOSUD010000008        42330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_638_length_42330_cov_23.913116, whole genome shotgun sequence.
ACCESSION   DAOSUD010000008 DAOSUD010000000
VERSION     DAOSUD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 42330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42330
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_42330_cov_23.913116"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1703
                     /gene="rny"
                     /locus_tag="PLG49_02155"
     CDS             150..1703
                     /gene="rny"
                     /locus_tag="PLG49_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ16148.1"
                     /translation="MDALLVVVLLIVTAIIVGVIAFIMGVSYRKRIAEAKIGSAEEKA
                     SKIIDDAITAAEAKKREIMLEAKEETLKAKNELDQEIRERRAELQRLERRIIQKEEAV
                     DRKAESLEKKEESLAEKMEELDKLKEEVAVLQQKEIQELERISGLTSEEAKEYLFRTV
                     ENQVKMESAILIKDIESKAKMEAEKKAREIITNAIQKCAADHVADTTVSVVPLPNDEM
                     KGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSAFDPIRREVARIALEKLILDGRI
                     HPARIEEMVEKAKKEVENMIREEGEAATFETGVHGLHPELVRLLGKLKFRTSYGQNVL
                     KHSIEVAHLCGIMAGELGLDVRLAKRAGLLHDIGKAADKEMEGSHISIGVELCRKYRE
                     SSVVINAIEAHHGDVEPESLIAVLVQAADTISAARPGARRETLETYIKRLQKLEEIAD
                     GFDGVEKSFAIQAGRELRIMVIPEQTDDAQMVLLSREIARKIESELEYPGQIKVHLIR
                     ETRAIEYAK"
     gene            1885..2145
                     /locus_tag="PLG49_02160"
     CDS             1885..2145
                     /locus_tag="PLG49_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOQ16149.1"
                     /translation="MEILKVASKSNPNAVAGALANTIREKNGAELQAIGAGALNQALK
                     AVIIARGYVAPSGIDLICIPAFTDIEINGEERTAIKLIIQAR"
     gene            complement(2195..2362)
                     /locus_tag="PLG49_02165"
     CDS             complement(2195..2362)
                     /locus_tag="PLG49_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOQ16150.1"
                     /translation="MQRPSQQVIQCNVKSCKYNDKTKYCTLDNILVGEHVTNAKTKHE
                     TDCMSFEPEME"
     gene            2462..3394
                     /locus_tag="PLG49_02170"
     CDS             2462..3394
                     /locus_tag="PLG49_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOQ16151.1"
                     /translation="MFIFDAHCDTLTKAMDKKEYLKQNNCQVDLKRLSQFSSPVQFFA
                     ICLHPKEYTNPLIEVLKIIDYFYQETEKNKELIQPALSYIDIENNIKNNKISGILTLE
                     GGEVLEEGLDVLWLLYQSGIRSIALTWNYKNRLGYGAMEKEEKGLTDYGKAVIKEMNQ
                     LGIIVDVSHLSYKGFWDVEKIATKPFIASHSNVQSICCHPRNLTDTQIQAIANAGGVI
                     GINSYPLFLNSSGKATLKDFLLHIDYIVNLVGIDFVGLGCDFDGIESYTEGLEDLSAI
                     KKLSSLLIKEYGNKGAEKILGLNFLRVVKEVLNK"
     gene            3479..4672
                     /locus_tag="PLG49_02175"
     CDS             3479..4672
                     /locus_tag="PLG49_02175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ16152.1"
                     /translation="MELSKKAKNIKPSSTLAVTAKAKKMKAEGIDVVGFGAGEPDFDT
                     PEHIKEAAIQAISDGFTKYTPASGTDELKKAICEKFKKENGLDYAPNQIVISNGAKHS
                     LTNVFMAILNAGDEVIIPGPYWLSYPEMVRLADGVPVIVHGRKEKDYKVTAEDLEKYL
                     SNKTKAIVINSPNNPTGVVYSKDELKEIADFAVKHDLYVISDEIYEKLIYDGLEHVSI
                     ASFNEEIYKRTIVINGVSKSYAMTGWRIGYLAAVPEVAKAISNLQSHATSNPNSIAQK
                     ATVAALTGPQDCVEKMRIEFEKRRNYMLERLSKISSFSVVKPQGAFYVFIDISQLVGK
                     QFAGKSIENADVFSDILLESEKVAVVSCTDFGYPSCIRLSYAISLENIQKGLDRIENF
                     VNQLG"
     gene            complement(4768..5727)
                     /locus_tag="PLG49_02180"
     CDS             complement(4768..5727)
                     /locus_tag="PLG49_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ16153.1"
                     /translation="MGEAIFVFSLFFIFLIILSLSSIRIIRQSTVGIVERLGSFHKKA
                     ETGINFLIPFIDRMKAIVDLRERVIDFPPQPVITKDNVTIQIDTVVYFKVTDPVFYVY
                     EIANPIAAIEYLTATTLRNIIGELDLDETLTSRDIINSKLRVILDEATDKWGIKVNRV
                     ELKNINPPRDIQDAMEKQMRAERERREAILRAEGEKSSAILRAEGHKEAAILKAEAEK
                     EANIRRAEGEKQSQILKSEGQAKAILNIQTAKAEGLKRMLIAMKESQPDESVLAIRSM
                     EALEKIAQGKSSKLILPSEAVNLLASVKSIKEVLTDANEEVKE"
     gene            complement(5757..6203)
                     /locus_tag="PLG49_02185"
     CDS             complement(5757..6203)
                     /locus_tag="PLG49_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOQ16154.1"
                     /translation="MEQMWAIWLIAAIVFAIIEISNTSFFMIWFSVGALAAMITAFII
                     PNIVIQFIVFLIISCILLFLTKNITKKFVQNKSKHKTNVDKLISAQGIVIEEIDNSKG
                     TGLVRVNGEIWSALSESNTIIPVDSKVIVINIQGVKLIVEKDVTLE"
     gene            6351..7151
                     /gene="proC"
                     /locus_tag="PLG49_02190"
     CDS             6351..7151
                     /gene="proC"
                     /locus_tag="PLG49_02190"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOQ16155.1"
                     /translation="MNKIGFIGAGNMGYAMLKGLLSYKNKEDLLFTDKSAERVNWVKN
                     NLNVHAFYSNKELVNSAKYIVMAIKPQDYSFVLEDIKAVFTKEHILISIAPGISIASI
                     KQVLGEDAKIVRAMPNTPALVGAGMSVISFSEDSFTVSERNEIFKIFSSFGEVEEIEE
                     KLMNAVVPISGSSPAYVFMMIEAMADGGVLAGLPRKLSYKLAAQAVMGSAKMFLESQT
                     HPGELKDAVCSPAGTTIEAVAVLEKRAFRSSIIEAMKACLDKANQMKK"
     gene            7163..7576
                     /locus_tag="PLG49_02195"
                     /pseudo
     CDS             7163..7576
                     /locus_tag="PLG49_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
     gene            7773..8834
                     /locus_tag="PLG49_02200"
     CDS             7773..8834
                     /locus_tag="PLG49_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOQ16156.1"
                     /translation="MIHIREGLVVKILNDDKDIIEMLVNVNQKSYKAIAYPAITGRLS
                     AGDRVVLNTTAVDLNLGTGGYHFVIMNKSCKQLTFENRGHLMKLKYTPIQIKTKTVEE
                     EYPQIINDFTSLKGMPIITGSIHSMLPAVVAVIKAKKKDAKIAYIMTDGGALPIAFSH
                     IVRDLKKRALIDCTISCGNAFGGDYEALNLYTALIAAKEIAKADVIIVIMGPGHAGTG
                     TKFGFTGMEMANNAHIIASLGGIPICIPRVSFADKRKRHYGISHHFLTAIGLHCLVPC
                     EIAFPIFPENKKRYIVHQYKKVNLDKKHKIYFLEEDTIAIMKKNQLCIQTMGRSLDED
                     PYFFKTAGACAVLLSKLLF"
     gene            complement(8823..8963)
                     /locus_tag="PLG49_02205"
     CDS             complement(8823..8963)
                     /locus_tag="PLG49_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16157.1"
                     /translation="MKAIIKNNQLTCIGKITDIIYFLTEIQAQYDTVKEYIDDKLKIL
                     KK"
     gene            9089..9634
                     /locus_tag="PLG49_02210"
     CDS             9089..9634
                     /locus_tag="PLG49_02210"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ16158.1"
                     /translation="MTTEFRRISREEIYKGKILTLVKDRVLLPNNKEADREIILHKGA
                     SAVVPVDEAGKIIFVRQYRQPIEEELLEIPAGLLEEGEDPLEAAKRELEEETGYRASQ
                     MRLICSMVSSAGFCNEIVHIYLATELYKGKQNLDEDEFVKIESYSLDDAIRMIFDGRI
                     KDSKTIAGILGTKQLLEDNKK"
     gene            9704..10318
                     /gene="spoIIM"
                     /locus_tag="PLG49_02215"
     CDS             9704..10318
                     /gene="spoIIM"
                     /locus_tag="PLG49_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOQ16159.1"
                     /translation="MKTNKRTISRKESLYGICIFSFLLGIITGAIFANYIDNFQNDEL
                     IQYMNHYFIHFNAKAISHSILLKQALFNHGKTFILIWIFGLGVAGIPFIVINIFLKGF
                     SYGFTSAFLFIHYGVNGLLFSLLSFLPQSFLLVPGMILISAASMNFALSGSNHKNYHK
                     DKKEKWSEYLLVLLVGLVIAVIISMMETFISPLLINIIIKRMSG"
     gene            10526..11416
                     /gene="xerD"
                     /locus_tag="PLG49_02220"
     CDS             10526..11416
                     /gene="xerD"
                     /locus_tag="PLG49_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOQ16160.1"
                     /translation="MEDFVEKYAKYLRDIKGASENTILSYQRDLKQFISFLKKVGIKE
                     IQKVNRTNIVAYLLELQKKGRAPSTVSRNIVSIRSFFQFLLKMHIVDEDPTENIESPK
                     QEKKLPEVLTIEEVDLLLRQPNENDLKGIRDKAMLELLYATGIRVSELISLQKSDVNL
                     TLEYIKCTDHEDSKERIIPLGASALRSLRNYMDKARFAMIKDSNEKYLFVNCSGTPMT
                     RQGFWKLIKVYAKKAKINKTITPHMLRHSFAAHMVANGADLQSVQEMLGHSDISTTQV
                     YAQLNKNKLKEVYTKAHPRA"
     gene            11543..12718
                     /locus_tag="PLG49_02225"
     CDS             11543..12718
                     /locus_tag="PLG49_02225"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOQ16161.1"
                     /translation="MIKRVIWIILDSVGMGAMPDADKYGDAGSNTIANVYKKSGGLQI
                     PHLIQLGLGNIEGMKELPKINEPVGCYARLAEKSNGKDTITGHWEMAGIITERPFPTY
                     PDGFPKEVIEAFEEKAGVKTLGNKVASGTEIIDELGEEHMRTGYPIVYTSADSVFQIA
                     AHEEVIPIERLYEICSIARELLTGEHQVARVIARPFIGEKKGSFIRTANRRDYAILPP
                     HETILDKIKAKNMDVIAIGKINDIFCGQGTTEHLHTQDNMDGVDKTIEFIKKDNRGLI
                     FTNLVDFDSKWGHRNNYEAYGKGLEDFDSRLPEIIAAMKDGDVLMINSDHGCDPTFPG
                     TDHTREYIPFVAYGKGLKQNVDLKTRDSFADIGQTIGELLGTEKIKNGISFAKEIIL"
     gene            12805..14106
                     /locus_tag="PLG49_02230"
     CDS             12805..14106
                     /locus_tag="PLG49_02230"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOQ16162.1"
                     /translation="MRMYDLIMKKRNGGVLSKEEINFIVKGYVEGSIPDYQVSAFLMA
                     VYFKGMNKEETSNLTWAFIHSGDIIDLSPIQGIKVDKHSTGGVGDKISLIVIPLVASL
                     GIPIAKMSGRGLGHTGGTIDKLESIKGFRTQMDIDEFIHNVNTYKMAIVGQTANLTPA
                     DKKIYALRDVTATVDSIPLIASSIMSKKIAAGSDAIVLDVKVGSGAFMKNLEDARELA
                     QTMVDIGKSLNRRVVAVITNMDQPLGHEVGNANEVKEAIEVLKNNGAEDEKTVALTIA
                     SYMAVLGGAYNNFEEAYMNLERKIETGEALEKFKEFIRIQGGDEEVAENPEKLPEAKY
                     HIEIKAQSEGYVNEINAEMIGNAAMLLGAGRKTKEDQIDYSAGISLVKKIGNKVMTGE
                     TIAILHTNKKEIKEAMDLVRDAYKISENQPAKVKYIYDVIM"
     gene            14203..15378
                     /locus_tag="PLG49_02235"
     CDS             14203..15378
                     /locus_tag="PLG49_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOQ16163.1"
                     /translation="MKKRLSMMLVFILFLNIFSFDFVKGEETNKDITLEIESPSAVLM
                     EAKSGKVLYEKNMNEKRFPASTTKIMTLLLIYEAVEKGQISWDDKVVVSEHAASMGGS
                     QVFLEPNEEQTVATMTKCIAVASANDASVAMAEHIAGSEEEFVKLMNEKAKELGMKDT
                     HFVNASGLHDADHVTSAYDIALVSRALITKYPEIHKLTTIWQDHIIHKTRRGEEEFGL
                     TNTNNLIKWYPGANGLKTGFTPESMYCLSGTAERDGLSLIAVVMGAKSKQARNSEVAK
                     MLNYGFANFTIIKGQPAGTVVGTVEVAKGNPDTVEGVIKKEVQLLVNKKLANEKIEFK
                     LELPESIQAPVTKGMKIGEIVYIFKGKEIARSDIVAATDVKKAGFKEMVNSLLKNWF"
     gene            15535..16167
                     /locus_tag="PLG49_02240"
     CDS             15535..16167
                     /locus_tag="PLG49_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOQ16164.1"
                     /translation="MENPLILLLVKVPGIVFGFTFHEFAHGLVAYLFGDNTPKKEGRL
                     TLNPLAHMDIIGLIMIFYMNFGWAKPVHTNPSNFKKRKLATLLVSLAGCVANILLAVL
                     FAGLLKLLFVFNVDTEKFSLLYISVYYAITINLVLGIFNLIPLPPLDGSKILFNLISP
                     RSYFKIIRYEYILQILLIVLVVTGIIPALVNPVIDYCLVLLEMLYKIKLI"
     gene            16198..16980
                     /locus_tag="PLG49_02245"
     CDS             16198..16980
                     /locus_tag="PLG49_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOQ16165.1"
                     /translation="MSITVKLEVFEGPFDLLFHLIEKNKINIYDIPIALITDQYFEYI
                     NSLEYRDMENMSEFLVMAATLLEIKSKMLLPSLKSEEEEEKDPREELVNKLLEYKKMK
                     YISQELKERQEEAQKVFFKESVLPPQIKALLLENDESDISEVLEGVSLKQIFLVFQDL
                     LKRNERKTDPIRSNFKSVKRDIYKVEDKITYILDLLYLSPVLSFKELFSDNCNKMEII
                     VTFLALLELIRHKKIKIKQERPFGDILITTKDRVDADETGAN"
     gene            16961..17545
                     /gene="scpB"
                     /locus_tag="PLG49_02250"
     CDS             16961..17545
                     /gene="scpB"
                     /locus_tag="PLG49_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOQ16166.1"
                     /translation="MKLEQTEAVIEAILFVSGEAVSLNKLSDIINEDLEKTKVIVEHL
                     KKKYEEEARGMRIIEINEAYQMCTSPELFEYIKEFYKNPKKFTMTQAALETLAIIAYR
                     QPVTRAQVEEIRGVNSDHIINKLIEYNLVCELGRMDAPGKPILFGTTEDFLRYFGFKS
                     IEELPQLEEELLKQIENEVNDEVKQIGLFDDQEN"
     gene            17645..18175
                     /locus_tag="PLG49_02255"
     CDS             17645..18175
                     /locus_tag="PLG49_02255"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOQ16167.1"
                     /translation="MKFVIHYDKTADAHRFYLSGYLFNYVKILNIDLKDYFINLLSHF
                     LKEDDNNSNITKSYINYAQAAKIIWCIWKKSKKHIQIPFIGIEIEIANENPALTALLF
                     GIMNACIPAAIYLLDEKISISDYRILIVPNFDLEKMNIKILAEIKMDLILLILIYIQI
                     KRRIKKNDKFQSATSD"
     gene            18144..18641
                     /gene="ytfJ"
                     /locus_tag="PLG49_02260"
     CDS             18144..18641
                     /gene="ytfJ"
                     /locus_tag="PLG49_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOQ16168.1"
                     /translation="MTNSSQQHPIESLLKAAMENIKEMIDVNTVVGNPVENSEGIMIL
                     PITKATFGFAAGGAEYGKLKENTSEDESKASSKRYPFGGGSGAGISIQPVAFMVVEKN
                     HVQIMHIHHNFGTISKILEEGPNFLTKLENCLNLRNKKKIKAEEGTKEKGGRVISKIV
                     EIEET"
     gene            18696..19859
                     /locus_tag="PLG49_02265"
     CDS             18696..19859
                     /locus_tag="PLG49_02265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOQ16169.1"
                     /translation="MKKRLLAMIVIITLIFNIYPLSLYGSEIKEPVIEAKAAILMDAK
                     TGRILWEKNAHEPLAMASTTKIMTCILALEKGKLTDEVVVSKKAASAPRVKMYLQAGE
                     KQLLGDLLYALMLQSYNDAAVAIAEHISGSVEEFCEEMTKKARAIGAKNTVFKTPNGL
                     DAQGHQSTAYDLALITKYAMQNPKFVEIINTKEYIIPQKGNYKSYHLRNANRFLNEFP
                     GANGVKTGYTGKAGYCFVGAARQGEMQLISVVLASGWGPRGKEQKWIDTKRLMKYGFD
                     YYKIETLLEKDKVLKTISVEKSKDEQVSLYTDRCIEYAIRDEEKSKIRVELELPGMIE
                     APVEKGEKLGEARVYIGDEFCGSVNLLADRSIERHDFPNCLKKVLNNWLKIIH"
     gene            20029..20736
                     /locus_tag="PLG49_02270"
     CDS             20029..20736
                     /locus_tag="PLG49_02270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ16170.1"
                     /translation="MRLQKFLANAGIASRRKAEEYILEGKIKVNGQVVTELGTKINPD
                     KDLVYYNNQIVSLPKEKIYLLLHKPEKYVTTVRDQFNRPTVLDLLPPALKAVHPVGRL
                     DYNSSGLLLLTNDGDFTYKITHPKHHIRKIYITKVKGIPAEASLDKLRKGLVIDHYKT
                     SPAKVKLLGKSKTEAVLEITIYEGRNRQVRKMCEAIGHPVISLKRIAIGKVFLGNLPV
                     GEYRFLTKQEIDYFMSL"
     gene            20774..21337
                     /locus_tag="PLG49_02275"
     CDS             20774..21337
                     /locus_tag="PLG49_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ16171.1"
                     /translation="MFIDLKLTDMVHEILKGKIREGDTVVDATVGNGNDTLFLARAVG
                     KTGKVIGFDIQKKALHQTQNKLIQEALADRVLLIHDSHSNMDQYIKEPIHGAMFNLGY
                     LPQGDKNIITKADTTIEALKKVCSLLLTGAFITIISYWGHQGGLEEKEAVEDFLTKLE
                     AKKYICAKFNYLNREGCPPIIYFLQRL"
     gene            21465..23009
                     /locus_tag="PLG49_02280"
     CDS             21465..23009
                     /locus_tag="PLG49_02280"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOQ16172.1"
                     /translation="MSTLSKVNELNSLRSKIMEGGGEAKIKKLHDEGRLTARERIEKF
                     LDENSFVEMGLFVRHRSTDFNMSQKETPADGVVTGYGTVEGRLVYVYSQDATVLGGSV
                     GEMHAKKIINLYDQALKMGAPIVAFLDSSGVRLQESVDALDGYGSLFVKQTMASGVIP
                     QITVVMGDCIGGAAFNTGLSDFTFMVSKKGRLFVNSPNTIEGPESKKANFESIGSATM
                     HSEKSGLVHFQYDDELECINNVRKLVSYLPSNNLEEAPYYNVTDDLNRVDSVLNSIVP
                     DDLNAAFDIKTVICALADDREFMEVKAKYAKNLVTGFLRLNGTTVGILANQSLENEGL
                     LDIDASKKGAEFINFCDAFNIPIVSITDAGGFKASLEEEQRGIVKYVSKLIYAFTNAT
                     VPKVNIIVRKAFGTAYIAMNSKHIGADMVFAWPSAQISVMNPEGAVNIMYADELKAAS
                     DPAALRAEKVKEFSEMQSSPYTVASRGYIDDIIEPAATRKRVIAALEMLYSKREERPA
                     KKHGSL"
     gene            23048..23392
                     /locus_tag="PLG49_02285"
     CDS             23048..23392
                     /locus_tag="PLG49_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOQ16173.1"
                     /translation="MGDLTHGLMVTVIGMGITFIVLLVLSYVLDLFRILSKGPSSKKA
                     DAEEKAEKIKPEPVENISIAEETNSKNDLELVAVITAAIAASLNTTSDQLVVRSFRRI
                     GNRSSQWNNLFY"
     gene            23416..23805
                     /locus_tag="PLG49_02290"
     CDS             23416..23805
                     /locus_tag="PLG49_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ16174.1"
                     /translation="MRRFQVTVNGNIYEVEVEELQGGQVAAPAAPTPVAAPSPAVKVE
                     APKAAPQPAPGGTEGAHKVTSPMPGTILDIKVNIGDSVKVGDTVAILEAMKMENEIVA
                     TADGKIASINVSKGTQVNTGDLIVTIG"
     gene            23845..25017
                     /locus_tag="PLG49_02295"
     CDS             23845..25017
                     /locus_tag="PLG49_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ16175.1"
                     /translation="MSFIEMLLETMRDLLAESGFAGLTFKHVIMIVISLGLMYLAIKK
                     EYEPLLLLPISFGMLLTNLPAAGLMAEPANGEVGGILYYLYQGVKLGIFPPLIFLGVG
                     AMTDFGPLIANPRSILLGAAAQLGIFFAFLGALGLKYIVPFINFTGAEAASIGIIGGA
                     DGPTAIYLTSRLAPHLLGPIAVAAYSYMALVPIIQPPIMRALTTKEERMVKMEQLRPV
                     SKTEKILFPIIVTILVSLILPSAAPLVGMLMLGNLFRESGVVGKLVDTASNALMYIIT
                     IFLGTTVGATASAENFLDPKTLGIIVLGLLAFSVGTAGGVLFGKIMYRTSGGKVNPLI
                     GSAGVSAVPMAARVSQKVGKEENPTNFLLMHAMGPNVAGVIGSAVAAGVLLSIFGA"
     gene            25059..26474
                     /locus_tag="PLG49_02300"
     CDS             25059..26474
                     /locus_tag="PLG49_02300"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOQ16176.1"
                     /translation="MSDKNNVRPVKITDTTFRDAHQSLIATRMTTEDMLPIAEKMDQV
                     GFHSMEVWGGATFDSCLRFLKEDPWERLRKLRATIKNTKLQMLLRGQNLLGYRHYADD
                     VVEYFVQKSIANGIDIIRIFDALNDTRNLETAIKAANKEKGHVQVAISYTLSEVHTLD
                     YYVKLAKELESMGAHSLCIKDMAGLLLPYQAYELVSALKKAVKIPIQLHGHYTSGVVA
                     MSYLKGIEAGADVVDTAMSPFSMGTSQPATEVMVETLKGTPYDTGLNQELLAEIADYF
                     RPLREKAMEEGLLNPKVLGVDIKTLLYQVPGGMLSNLVSQLKTQNAEDRFYEVLEEIP
                     RVRADFGYPPLVTPTSQIVGTQAVLNVLMNERYKMVTKESKALVKGEYGRTPVPISEE
                     IVKKVIGNEKPITCRPADLIEPELEKIESEMKQYKEQDEDVLTYALFPQVAEEFFKYR
                     QAQKTKVDVNLADLENKVYPV"
     gene            26682..27593
                     /locus_tag="PLG49_02305"
     CDS             26682..27593
                     /locus_tag="PLG49_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOQ16177.1"
                     /translation="MYNGNDLIQRIHKNLPKLSKGQKLIANYILNHYEKAVFLTAAKL
                     GAVVGVSESTVVRFANVLGYDGYPKLQRALEELVKNKLTSVQRMEVAYDRLDQKSVLK
                     YVLQSDADKIKHTLEEIDETSFDNTVNAILNARKIYILGVRSSAALASFLGFYFNLLF
                     DNVKLIHTNSVSEMFEQIHKMNSDDVVIGISFPRYSKRTIKAMEFAKSQKATVIAITD
                     SPLSPLVRYADYSLLARSDMASFVDSLVAPMSVLNALIVAISLKKKDEISETLSTLEK
                     IWTEYQVYDNEDEDLNLTYSLNRRQGG"
     gene            27595..28215
                     /locus_tag="PLG49_02310"
     CDS             27595..28215
                     /locus_tag="PLG49_02310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ16178.1"
                     /translation="MLRLYLVRHGETKWNIENKYQGASDIPLSPVGIEQAQALARKIK
                     AFKIDKIYSSDLSRAYETAKMIAKEKNLEIECMPGLREISFGEWEGYTIAQLKELYGD
                     SYKKFFLEPHKYPFPGEGSMVAVQTRITKAVSEIVKKHPRGDILIVSHGGVLKVLIMS
                     LLKIDLSFFKSFWLGNTSLSIIKKTDDDRWVLSLLNDLSHLEELNY"
     gene            28258..28620
                     /gene="aroH"
                     /locus_tag="PLG49_02315"
     CDS             28258..28620
                     /gene="aroH"
                     /locus_tag="PLG49_02315"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOQ16179.1"
                     /translation="MSVCAIRGAITIEENTKENILENTSLLLKEIIHKNNINIDDIIS
                     ITFTATDDIDAAYPAVAARNLNITKAALLCFQEMKVIDSLKMCIRVMLLVRSSKSQDQ
                     VHHVYLKNAVVLRPDLIH"
     gene            28638..29294
                     /gene="cmk"
                     /locus_tag="PLG49_02320"
     CDS             28638..29294
                     /gene="cmk"
                     /locus_tag="PLG49_02320"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOQ16180.1"
                     /translation="MERFAIAIDGPAGAGKSTIAKLLAKRLNIIYVDTGAMYRAVALY
                     CIENAFDYNKKEDVERALENIDIKIVYQDDNQRILLNNRDVTDLIRTGEVSKGSSAVA
                     TIYAVRIKMVELQRNIAKENSVVMDGRDIGTYVLPDANLKIFLTASVEERARRRYNEL
                     MEKGIECNLEQIKKEIEQRDKNDSSREFAPLRKADDAIEIDTTNLTIEEVVEKIIALL
                     "
     gene            29417..30538
                     /gene="rpsA"
                     /locus_tag="PLG49_02325"
     CDS             29417..30538
                     /gene="rpsA"
                     /locus_tag="PLG49_02325"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOQ16181.1"
                     /translation="MNDLENHNNESFEALLNESFVILRSGEIVKGTVINVTDTEVMVN
                     LGYKSDGIISKAEFSNDPHINLKNAVKVGDEIEVYILRVNDGEGIIELSKKRVDIQKG
                     WDILQKAYEAESILTGRVVEAVKGGIIVLNHEVRIFIPASLLGARYIQDLNQFLGQTI
                     HFKIVEFDKKRRKIIGDRKQVLVEERKIKREQLLNSLEVGDLVTGRVSNITGFGAFVD
                     LGGIDGLIHSSELSWKRISKPSDILKIGQEVKARVIQIDKENEKISLSLKKEEENPWT
                     NVEQKYPVGSVVKGKVVRMVPFGAFVELEDGLDGLIHISQIANKHVANAEEELQLGQI
                     IEAKVMEVDEKQKKISLSIKEIKKETEQDNEKENELSQE"
     gene            30590..31372
                     /locus_tag="PLG49_02330"
     CDS             30590..31372
                     /locus_tag="PLG49_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOQ16182.1"
                     /translation="MIVTYEDFKKEIYHMSGIDLNCYKERQMKRRIDALIKKNNYNDY
                     HSYVLALKDNKELYNEFINYLTINVSQFFRNPSQWEVLEKEIIPYLLKSSSDLKIWSA
                     ACSTGDEPYSLVMLLSKFIPLSKIKILATDIDKEALEKAKMGLYNDKSIEGMPKEFFN
                     KFFKPVGEFFQISEEVKKCVTFEHHNLLSDRYPQNCDLIVCRNVLIYFTEEAKNNIYK
                     NFNKSLKMDAILFVGSTEQIIMSHRYGFTPVKSFFYKKIAEL"
     gene            31398..31718
                     /locus_tag="PLG49_02335"
     CDS             31398..31718
                     /locus_tag="PLG49_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ16183.1"
                     /translation="MIYIIISMIIWGSIGLFIRWINMSSIEIAFLRAGIAACFLLFYS
                     FFYKKIDMKKIKENIILLILAGAAVAFNWIMLFQAYRFTSIATATICYYFAPVIVVLL
                     SPFV"
     gene            31781..32266
                     /locus_tag="PLG49_02340"
     CDS             31781..32266
                     /locus_tag="PLG49_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ16184.1"
                     /translation="MMYYSPENVGRGSNNLLGILFGLLAAVFYALVVVLTKKMKDIES
                     FEITCIEIFVAALVLLPFVLYHPQLHIKNIQTGIIVCILGLIHTGLAYILYFKGIRQV
                     KAQNAALLSYIDPLSAICFSTVFLGEIPNKAQVIGALFIFMASFIHEIKIKNPQIKQK
                     A"
     gene            complement(32280..33758)
                     /locus_tag="PLG49_02345"
     CDS             complement(32280..33758)
                     /locus_tag="PLG49_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16185.1"
                     /translation="MTNNSKESLELILNNSFKESVPLAFLKLLNHSVHLYINESLTYI
                     NDINENLYEMSNYAINLFLNLEDVLKENGEEKFKKIETIINYKEKLEKLYRILYAYYT
                     ELNIATLILNDKNNLLSSKDNNIESLDFNLFFEDCFVFINEFISPDDLESVLCQLLSS
                     IPIRVSKNKYYEYVEKSLLLINEPEERLKDIFEFLKEQFNGKHVKGYHDFLPDIAMSI
                     ENCIEKALEKPNKDEIEGILKEIESIEDNLLNLGDLLLSLYDIFNCMSVLLMLQNVDL
                     ESLYNRHVAYKDFYLSSSSLFNEDTDPHEKEILIETLPDLMNQHIGILENNLLKINRK
                     IKDILSNESLKIDSHEELSNIFENHLLIFSHLNQRLMDTLVYNTKNQKDLSVNKELEK
                     YIQKFIQYLDSEFKNMTNPLRKARMQNFLGLIPLAMNSDEFMDYLINAVNMSSDKNKA
                     RIVDNIGRIMAYYGFFDNHHCDCNHDHDENCHCHHSHDHKNH"
     gene            33907..35505
                     /locus_tag="PLG49_02350"
     CDS             33907..35505
                     /locus_tag="PLG49_02350"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ16186.1"
                     /translation="MYKVLCIDEEKNQVNKIVKMIVDSGLDFTVLKTVSTRKEALDFL
                     EKNQLDLIVSDIRLPDYNGYQLFKDLKESHPEIKMILYTSYNEYEYVQKALEEGLIDY
                     IFKPVKEQELRRSLIRAKQLLDDISRRYEEQNRILAKFDESLPILQDRFLTNLIHGYP
                     EKESDIHKTFEYFNIMIPPGYTVMVLKIDYYDKLSLIMEEKDKHMLIFSALYKIESIL
                     EEYKNGIAFINRHDEIVIILGGKLSINESITIGEKIRKSIETDLKITSTIGIGKMYNE
                     ARKINISYKQAKAAIRYNYYLGFNSVIHINYVEPTNIITYEYPLKKEEYLVYETVIGN
                     EKRVLELLEDLFAVLKACQPLPDKLLPKIILDIIVSINRYASEQGINIDSFFSENFSM
                     KDVLKHKEHNDAFNYLSNLLPAICKFIKEKRKKNEEAILVKAKMYVEENFLSNITLNQ
                     TAMHVQTTPENLSVILAEAGCRSFYEYCTKIRIEKAKSLLEETNLSDEGIALKIGYED
                     RNYFRKIFEQMEGMTTDEYRLLKK"
     gene            35675..37885
                     /gene="glgB"
                     /locus_tag="PLG49_02355"
     CDS             35675..37885
                     /gene="glgB"
                     /locus_tag="PLG49_02355"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOQ16187.1"
                     /translation="MKTTVNVTEMYEIIHSEHRDPHHILGMHEIRIQRKKAIAIRAFI
                     PQALSIKVIEENTNKKYDMGKIHESGFFEIVFLNKKEKFRYQLEIMDYHNHQWTIYDP
                     YAFNPIISDLDRYLFNQGTHYKIYEKLGSHPMTVDGVKGILFAVWAPNAKCVSVVGSF
                     NDWDGRRHPMRCLGDSGIWELFIPGVLEGDLYKYEIKTKENQLLLKTDPYGFYTELRP
                     STASIVYDIDKYQWHDEKWMKERAQKDPLNQPLSIYEVHLGSWMRVVEENNRFLTYRE
                     AAHKLVAYVKDMGYTHIQLMPIEEHPYDGSWGYQVTGYYAPTSRYGKPEDFMYFVDYC
                     HQNGIGVILDWVPGHFPKDAHGLARFDGTALYEHEDPRKGEHPDWGTLIFNYGRYEVK
                     LFLIANALYWFEKYHVDGLRVDAVASMLYLDYGRRDGDWIPNSYGGNENLEAVEFLKH
                     LNSIVYQYHPGIMMIAEESTAWPNVSRPTDMGGLGFGLKWNMGWMNDFLRYISKDPIY
                     RKYHHNDLTFGLVYAYTENFILVLSHDEVVHGKHSMIGKMPGDYWQKFANLRVAYGFM
                     YGHPGKKLLFMGGEFAQFDEWSEARSLDWHLLQFDKHREMQAYVRDLNRLYLKETAFW
                     ESDFSWEGFEWINGHDAESSIISFIRKGKEPQNYLIFVCNFTPVPRYQHRIGVPEKGK
                     YKEIFNSDASQYGGSNVINEQEIVSESIEWDGRENSIGLKVPPLGMVVLKRVDL"
     gene            38122..39342
                     /gene="argJ"
                     /locus_tag="PLG49_02360"
     CDS             38122..39342
                     /gene="argJ"
                     /locus_tag="PLG49_02360"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOQ16188.1"
                     /translation="MQIIDGGVTSPKGFMANGSHVGIKKKKKDLSVIYTVFPAKGAAA
                     FTTNVVKAAPVLWNQKIIHERGNIQAIVINSGNANACTGEKGMQDAMLMAKTAADALN
                     LKTEEVLVASTGVIGVPMPMDIICPGIKQTIEGLGDSREAALMAAEAIMTTDTYPKEI
                     AVSFTVQEKTVTIAGMAKGSGMIHPNMATMLSFITTDLNISYELLDKALKESIADSYN
                     MISVDGDTSTNDMVIVLANGAAGNDLIEEENEDYEVFKKALHYVNSHLAKEIVRDGEG
                     AGKFIEVQVKGTKTKEDARILAKSIITSNLVKTAFFGEDANWGRILCAMGYSGVQFDV
                     NHVTIEFRSKAGKIALMEKGLPILFDEELALKILKEKDIQVIACLQEGEEEATAWGCD
                     LSYEYVRINGDYRT"
     gene            39355..40215
                     /gene="argB"
                     /locus_tag="PLG49_02365"
     CDS             39355..40215
                     /gene="argB"
                     /locus_tag="PLG49_02365"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOQ16189.1"
                     /translation="MEKLIEKAKVLVEALPYIKEFYGKTVVIKYGGSAMIDERIKETV
                     IQDIVLMKLVGINPVIVHGGGPEINKVLEQMNKKSQFINGLRVTDKETMEVVEMVLSG
                     KVNKEIVSEIQNQGISSVGISGKDGGMLLAEKKLVDGYDLGYVGHVVAVNPRLLLTLI
                     QTDFIPVIAPIGIDKKGNSYNINADYAAVAVAGALKAEKLVFLTDVEGVLMDVDDSSS
                     VISQLSIKDVDRYIEKGIISGGMIPKVQCCVEGVKMGVKTVHILDGRIEHCLLLEIFT
                     HKGVGTMIMQ"
     gene            40333..41166
                     /locus_tag="PLG49_02370"
     CDS             40333..41166
                     /locus_tag="PLG49_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ16190.1"
                     /translation="MALVGDGIDSFTDIVTGFIILFAAKIMSKPPDREHPYGHQRAET
                     IATVLLAFIIFFAGAQLLINTVPAIFSKEETDLPSAIAIYIALISILGKLFLAFYQYK
                     IGKLTQSSMLIANSKNMKNDILISVGVLFGLLFTFLLKLPVFDFITAIIIALWIIKEA
                     YEIVIESNMELMDGVEDTSIYQEVFDAIDQIEGVYNPHRTRIRKMSNLYIIDTDIEVD
                     QNLTVLEAHKIAMKVEKIIKEKIQDVYDVIVHVEPLGNIEHNESYGLSQKMLREKKDS
                     Q"
     gene            41338..>42330
                     /locus_tag="PLG49_02375"
     CDS             41338..>42330
                     /locus_tag="PLG49_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ16191.1"
                     /translation="MWRFPSTIYVVKAGDSLYQIARQFGVSVQDIININGIVDTIIYP
                     GQRLIISIAYYLVQPGDSLYRLAQKFNTTVESIMRLNNLTNINLRIGQRLQVPQYSEA
                     IVRTALANIRRRPGVNFPIETVMVRGAKLPVTASSNGWYEVALYNGRRGFIAKSLVDF
                     NVYGSSLPLTNILGFYTLEEGPALPSSYESFINNTGEISQLALFMFRIDADNPTEIEK
                     FGGDFSNAYVEDLIRIAHENNIKICPVVHNLLYERGDVTVGKNTVKAMVATPESRAAF
                     IQNIIELIEGYGFDGVNIDIEDVYLEDSDRLTAFYRELGRALHQRGYFFSVSVPS"
BASE COUNT        14729 a         5753 c         8492 g        13356 t
ORIGIN      
        1 gaaatcagaa agatattttc gaataaacca ttattaaatg gatataatgt tcatttctag
       61 tacttgacat aatttttaaa aagtaataca atcattatgt tatactttac aattcgaact
      121 atgaaaactg aataaaggag gtgttatcaa ttgatgcatt attagttgta gtactattaa
      181 tagttactgc gataattgtt ggagttattg cctttataat gggtgtttct tatagaaaac
      241 gtattgcgga agcaaaaatt ggttctgctg aagaaaaagc tagtaaaatt attgatgatg
      301 caattactgc tgcagaagca aaaaaacgcg aaattatgct ggaagccaaa gaagaaacat
      361 taaaggcaaa gaatgaactc gatcaggaaa taagagagcg ccgtgcagaa ctgcagcgct
      421 tagaaagacg aatcattcaa aaagaagaag ccgttgatag aaaagctgaa tcgctggaga
      481 aaaaagaaga atctctggct gaaaaaatgg aagaattaga taaattaaaa gaagaagttg
      541 ccgttctcca acagaaagaa atacaggaat tagaaaggat ttctggactc acctccgaag
      601 aagcaaaaga atacttattt agaacggttg aaaatcaagt aaagatggaa tcagcaattt
      661 taattaaaga tattgagtca aaggcaaaaa tggaagctga aaagaaggct cgtgaaatta
      721 ttacgaatgc tattcaaaaa tgtgctgccg atcatgttgc agatacaaca gtatcagttg
      781 taccattacc taatgatgag atgaaaggga gaattatcgg gcgtgaagga aggaatatac
      841 gtacattgga aactctgaca ggtattgatt tgattattga tgatacgcct gaagcagtta
      901 ttttgtcagc ttttgatcct attcgaagag aagttgcaag aatagctttg gaaaaactga
      961 tattagatgg cagaattcat cctgcaagaa ttgaagaaat ggttgaaaaa gctaaaaaag
     1021 aagttgaaaa catgattagg gaagaaggag aagccgctac ttttgaaaca ggcgtacacg
     1081 gccttcatcc agaacttgtg cgtttgttag gtaaattaaa gttcagaacc agctatggtc
     1141 aaaatgtctt aaagcattct attgaagttg cgcatttatg cggaataatg gcaggggaat
     1201 taggccttga tgtccgactg gcaaaaagag ccggcttatt acatgatata ggtaaagcag
     1261 cggataaaga aatggaaggt tcacatatta gcattggtgt tgaattgtgt aggaaatatc
     1321 gagaatcttc tgttgtcata aatgcaattg aagctcatca tggggatgta gaaccagaaa
     1381 gtttgattgc agttcttgtg caggctgcag atacaatttc agcagcaaga ccgggagcaa
     1441 gaagagaaac cttagaaaca tatattaagc gtttgcaaaa attagaagaa attgctgatg
     1501 gctttgatgg agtagaaaaa tcttttgcta ttcaagcagg tagagaatta agaattatgg
     1561 ttatcccaga gcaaacagat gatgcgcaaa tggtattgtt atccagggaa attgccagaa
     1621 agattgaaag tgaactggaa tatcccggac agataaaagt gcatctgatc agggaaacaa
     1681 gagcaataga atatgcgaaa taaaaagcgt aatttaatta cgcttttttt attttaattt
     1741 tatttgaaat taataatgtc tctatgttaa atttatgata tataaccaat ttttttaaaa
     1801 aaatatattt ttaagaagga atttacaaaa ataagtagaa tatattaaag aagttaattt
     1861 tatctaaaaa agggggtcac aaaaatggaa atattaaaag ttgcttcaaa atcgaatcct
     1921 aatgctgttg ctggtgctct agccaatacc attcgtgaaa aaaatggggc tgagttgcaa
     1981 gcaataggag cgggtgctct caatcaagca ctaaaagcag tgattattgc acgaggatat
     2041 gtagcaccct caggaattga tttaatctgt attccggctt ttacggatat agagattaat
     2101 ggtgaagaaa gaacggcaat taaattaatt atacaagccc gttaaagtct atcttattat
     2161 taaaagcgtg cagatattgc acgcttttct ttgattattc catttctggt tcaaagctca
     2221 tacaatctgt ttcatgtttt gttttagcat tggtcacatg ttctccaact aaaatattat
     2281 ctaaagtaca atattttgtt ttgtcattat acttacaaga tttaacattg cattgaataa
     2341 cttgttgact tggacgttgc atagctttca actcctttta aaaatagtat ataattaata
     2401 aagcaatatc attatttgca agcagtcagt attttattct aagaaatttt cggaggtcat
     2461 tatgtttata tttgatgccc attgtgatac tttaacaaaa gcaatggata aaaaagaata
     2521 tttaaagcaa aataactgtc aggtggattt aaaaaggtta tctcaatttt catcacctgt
     2581 tcagtttttt gccatttgtt tgcacccgaa agaatataca aatcctctta ttgaggtatt
     2641 aaaaattatc gactattttt atcaagagac agagaaaaat aaggaattaa ttcagcctgc
     2701 tctttcatat atagatattg agaataatat aaaaaataat aaaatttccg gtattttaac
     2761 tttagaagga ggagaggtct tagaagaagg tctggatgtt ttatggcttt tataccagtc
     2821 aggaataaga agtattgcct tgacctggaa ttataaaaac aggcttgggt atggagccat
     2881 ggaaaaagaa gaaaaaggcc tgactgatta tgggaaggca gtcattaaag aaatgaacca
     2941 attaggtatc attgtggatg tgtctcattt atcttataaa ggtttctggg acgtagaaaa
     3001 aatcgcaaca aaacctttta tagccagtca ttccaatgta caaagcatct gctgccaccc
     3061 cagaaattta acagacactc aaatccaagc catagccaat gccggcggag tgataggtat
     3121 taatagttat cctttatttc ttaattcatc agggaaagca actttaaaag attttttgct
     3181 acatatagat tatatagtta atttggttgg aattgatttt gtaggtttag gatgtgattt
     3241 tgacgggatt gagtcttaca cagaaggctt ggaagatctg tctgcgatta aaaaattatc
     3301 ttctttatta attaaagaat atgggaataa aggtgctgaa aaaattttag gtttaaattt
     3361 tttaagagtc gtcaaagaag tcttgaataa ataaaaaaga ataattcaaa ttattttctt
     3421 gcaaatgtct gaaaaatata ttacaatatg aagaaaaaat aaatggatgg tgaatcaaat
     3481 ggagctttct aaaaaagcga aaaatataaa accttcgtca actttagctg ttactgcaaa
     3541 agctaaaaaa atgaaagctg aagggataga tgttgtaggt tttggtgcgg gagaaccaga
     3601 ttttgatacg cctgaacaca ttaaagaagc ggcgattcaa gccatttcag atggttttac
     3661 aaaatatacg ccagcttctg gtacggatga gctaaaaaaa gctatttgtg agaaatttaa
     3721 aaaagaaaat ggcttagatt atgcgcctaa tcaaattgtt atttctaacg gagcaaaaca
     3781 ttcattaaca aatgttttta tggccatttt aaatgcaggg gatgaagtga ttattcccgg
     3841 accttattgg ttaagttatc ctgaaatggt cagactggct gacggggtgc ctgtcattgt
     3901 tcatggaaga aaagaaaagg attataaagt aactgctgag gatttagaaa agtatttaag
     3961 taataaaacc aaggcgattg ttattaatag cccaaataat cctacaggcg ttgtatattc
     4021 taaggatgaa ttaaaagaaa tagctgattt tgcagtaaaa catgacttat atgttatttc
     4081 tgatgaaata tacgaaaagt taatttacga tggtcttgaa catgtaagta ttgcttcatt
     4141 taatgaagaa atttataaaa gaaccatcgt gattaacggg gtatccaaga gttatgccat
     4201 gacaggctgg agaattggtt atctggctgc agtacctgaa gttgcaaaag ccatttcaaa
     4261 tttgcaaagc catgcaacat ccaatccaaa ttctattgct cagaaagcaa ctgttgctgc
     4321 tttgacaggg cctcaggatt gcgtagaaaa gatgcgtatt gaatttgaaa aaagaagaaa
     4381 ttatatgtta gaaagattaa gtaaaatttc ttcattttca gtggttaaac cacagggcgc
     4441 tttttatgta tttattgata tttctcaact tgttggtaag cagtttgcag gaaaaagtat
     4501 tgaaaatgcg gatgtatttt ctgatatttt actggaaagt gaaaaagttg ccgtggtttc
     4561 ctgtacagat tttggttatc ctagctgtat aagattgtct tatgcgattt ctttagaaaa
     4621 tatccaaaaa ggtctcgaca gaattgaaaa ttttgttaat caattaggct aaaataaaat
     4681 acctcttgca atggattgga cttttaacag tcaatcaaat aagagactgt aaataaaagt
     4741 tcatatccat aagcaagagg tattttttta ttcttttact tcttcattgg catcggtaag
     4801 aacttctttt atgcttttta ctgaagccag taaatttaca gcttcagaag gcagaattaa
     4861 cttactggac ttgccttgag cgattttttc aagagcttcc atagaacgga tagcaagcac
     4921 agattcatcg ggttgacttt ctttcatagc aataagcatt ctttttaagc cttcagcttt
     4981 agctgtttga atgtttagta ttgcttttgc ctgaccttca gattttaaaa tttgagattg
     5041 tttttcacct tctgcacggc ggatattggc ttctttttca gcctcagctt ttaaaattgc
     5101 agcttcttta tgaccttcag ccctaaggat agcagagctt ttttctcctt cagctcgcag
     5161 gatggcttct cttctttccc gttcagcccg catttgcttt tccatggcat cctggatatc
     5221 tcttggagga ttaatgtttt taagttctac tcggttaact tttatacccc atttgtcagt
     5281 ggcctcatct aatatgacac gtaattttga attaatgata tctcttgaag taagagtttc
     5341 gtccaaatcc agttcaccga taatattacg caaagttgtg gctgttaaat attcgattgc
     5401 agcaattgga ttagcaattt cataaacata aaagacagga tctgtaactt taaagtagac
     5461 taccgtatca atttgaatgg ttacattatc tttagtaatc acaggttgag gaggaaagtc
     5521 tataactctt tctcttaaat ccacaatggc tttcatgcgg tcaataaagg ggattaaaaa
     5581 attgatccca gtttcagctt ttttatgaaa gctaccaaga cgctcaacaa ttcctaccgt
     5641 cgattgacgg ataattctta ttgatgataa agataaaata attaaaaaga taaagaagag
     5701 gctaaaaaca aaaattgctt cacccattta tattcctcct tttaaaatat tataaatcat
     5761 tcaagggtaa catctttttc tacgattaat tttacccctt gaatgttgat gacaatgact
     5821 ttagaatcaa ctggaatgat tgtattactt tcagaaagtg ctgaccatat ttcgccgttt
     5881 acccgtacca gaccagttcc ttttgaatta tcgatttctt caatcacaat accctgggca
     5941 ctaatgagct tatccacatt ggttttgtgt tttgacttgt tttgaacaaa ttttttagta
     6001 atatttttgg tcaggaaaag taatatgcag gatatgatta agaaaacaat aaactggata
     6061 acgatgtttg ggattataaa ggctgttatc atggcagcta aggctcctac agaaaaccaa
     6121 atcataaaga agctcgtatt actgatttct atgatggcaa aaacaattgc tgcaataagc
     6181 catatagccc acatttgctc cataatttca cccccttaat gtgtagtgta gagcaacact
     6241 ctacaattaa ttatagttta tattaacttt catgtcaatg atttcttaaa ttttttatga
     6301 tataatattt taagtattag atattttatt acttttgagg aggtttttca atgaataaaa
     6361 ttggatttat tggtgctggt aatatgggtt atgccatgtt aaaaggtttg ctttcttata
     6421 aaaataaaga agacttattg tttaccgata agtctgcaga aagagtgaat tgggtaaaaa
     6481 ataatttgaa tgtacatgca ttttatagta ataaagaatt ggtaaatagt gcaaaatata
     6541 tagtgatggc aataaagcca caagattatt cttttgtgtt ggaagacatt aaagcggttt
     6601 ttacaaaaga gcatatatta attagcatag caccaggtat tagtattgcc agcattaagc
     6661 aagtccttgg ggaagatgca aaaattgtcc gtgctatgcc taatacacca gccctggtag
     6721 gcgcaggaat gagtgttata agcttttcgg aggattcgtt tactgtaagc gaaagaaatg
     6781 agatctttaa gattttttct tcttttggtg aggtagaaga aattgaagaa aagcttatga
     6841 atgctgtggt acctatttct ggcagttcac cagcttatgt atttatgatg atagaggcca
     6901 tggcagatgg cggggtttta gctggtcttc cacgtaaact gtcttataaa ttagctgctc
     6961 aggcagttat gggttctgct aaaatgtttt tagaaagcca aacacatcct ggcgagttaa
     7021 aagatgctgt ttgttctcct gccggtacga caattgaagc ggtagctgtt ttagaaaaaa
     7081 gagctttcag aagcagtatt attgaagcta tgaaagcttg tttagataaa gccaatcaaa
     7141 tgaaaaaata aggagaatga ttatggaatg ttatgaaatt tataccgatg gtgcctgcag
     7201 taataatcag gcacaaggtc tgcaggcagg aggctgggca gctgtttttg tcaacggtga
     7261 ggaactttcg ggaggggata gggcaacgac caacaaccgg atggaaatga aagctgtcat
     7321 agaggctcta aagaataccc caccaggtgc tcatgttaaa atatattcgg attctgccta
     7381 tattatcaat tgttttaatc agaaatggat tgataagtgg gagaaaaatg gctggatgac
     7441 ttctagcaat aaagcagttg aaaataaaga tttgtggtta gaaatccgtc aattagaaaa
     7501 agaaagaaaa gtggaatgga taaaagtgaa aggtcattcc ggaaatccct ggaatgagag
     7561 ggctgatcag ttagcggttg ctgctattcc taaagaaggt gaaagtcata aaaataatag
     7621 taagaaaagc atcatatctt tgtctgaaga tgaaaaagta aatttaatag agttattaga
     7681 aaaaaatgaa agttttattc cccaatccct gaggggttta ttagctaaat taaaaaatct
     7741 ggaatgatga tatattttaa tggggcgttt atatgattca tataagagaa ggtttagttg
     7801 ttaagatttt gaatgatgat aaggatatta tagaaatgct tgttaacgtt aatcaaaaaa
     7861 gctataaagc cattgcctat ccagcgatta caggaagact ttcagcaggt gacagggttg
     7921 ttctaaatac tacggcagtg gatttaaatt taggaacagg cggatatcat tttgttataa
     7981 tgaataaatc ctgtaagcag ttgacttttg aaaacagagg tcatttgatg aaattaaaat
     8041 atacaccgat acaaataaaa acaaagacag tagaagaaga gtatccgcaa atcattaatg
     8101 attttacatc tttaaaaggt atgccaatca tcactggcag tattcacagc atgcttccag
     8161 ctgttgttgc tgtcattaaa gccaaaaaaa aagatgccaa aattgcctat attatgacgg
     8221 atggtggagc cttacccata gcgtttagtc atattgtaag agatctgaaa aaaagagcat
     8281 tgattgattg taccataagt tgtgggaatg cctttggagg agactacgaa gccttaaatt
     8341 tatatacggc tttaattgca gcaaaagaaa ttgcaaaagc cgatgttatt attgtcatta
     8401 tgggacccgg gcatgcaggg actggcacca aatttggttt tacaggaatg gaaatggcta
     8461 ataatgccca tataatagca tccttagggg gaattcctat atgtattcca agggttagtt
     8521 ttgcagacaa aagaaagcgt cattatggga tcagtcatca ttttttaacg gctatcggat
     8581 tacattgcct ggtaccatgt gaaattgctt ttcctatatt ccctgaaaat aaaaaaagat
     8641 atattgttca tcagtataaa aaagtaaatc ttgataaaaa acacaaaatt tattttttag
     8701 aagaagatac gatagcgatt atgaaaaaaa atcaattatg tattcaaacc atgggcagga
     8761 gcttggacga agacccttat tttttcaaaa ctgcaggagc ttgtgcagtt cttttatcaa
     8821 aattactttt ttaaaatttt taatttatca tctatatatt cttttacagt atcgtactgg
     8881 gcttgaattt ctgtcagaaa gtaaataata tccgttattt ttcctataca ggttaattgg
     8941 ttatttttaa taatagcttt caatgacatc ccacctttaa atgaagattt taaacttata
     9001 tatagcttat tcaaaagata taaaattcat tcatttaaat gatttaaaat aatacttata
     9061 aattattcaa tagatttgga ggattaagat gactacagaa tttcgcagga tttccagaga
     9121 agaaatatat aaaggaaaga tacttacttt agtaaaagac agggttttgt tacctaacaa
     9181 taaagaagct gaccgggaaa ttattcttca taaaggcgcg tcagctgttg ttcctgtaga
     9241 tgaagcagga aagattatct ttgtacgaca ataccgccag ccaatagaag aagaattgtt
     9301 ggaaatacca gctggtttat tagaagaagg agaagatcct ttagaagctg ccaaaaggga
     9361 attggaagag gaaacaggct atagggcctc acaaatgcgt ctgatttgca gcatggtgag
     9421 ttcagcgggt ttttgcaatg aaatagtaca tatttattta gcgacagaat tatataaagg
     9481 aaagcaaaat ttagatgaag acgaatttgt taagatagaa agttatagcc tggatgatgc
     9541 gataagaatg atatttgatg gcagaataaa agacagcaag actatagcag gtatacttgg
     9601 tactaaacag ctattagaag ataataaaaa atagtacaag cagttctatt cttttattag
     9661 ttttcatata tttactagag acgactacga aaggaagaga aagatgaaaa ctaataaaag
     9721 aacgatttcc aggaaagaaa gcttatatgg catatgtatt ttttcatttt tgcttggaat
     9781 cattacaggt gccatatttg ctaattatat tgacaacttt caaaatgacg aactcataca
     9841 atatatgaat cattatttta ttcattttaa tgccaaagcc atttctcatt ccattttact
     9901 aaaacaagct ctttttaatc atggtaagac ttttatcctc atatggatat ttggattagg
     9961 tgtagcagga attcctttta ttgtaatcaa tatattttta aaaggttttt cttatggctt
    10021 tacttcagca tttttattta tccattatgg tgtcaatggc ttattgttta gtcttttgtc
    10081 ttttttacct caaagttttt tgctggtacc aggaatgatt ttgatttcag cagccagtat
    10141 gaattttgct ttgtcaggtt ccaatcataa aaattatcat aaagacaaaa aagaaaaatg
    10201 gtcagaatat ttgttggttt tattggttgg attagttatt gctgttataa tcagtatgat
    10261 ggagactttt atttctcctt tactgattaa tattattatt aaaagaatgt ctggataaaa
    10321 aatttttatg gttaaaatgt ttaataatta atggatagat ttgtataggt ttttttgaaa
    10381 aaatatcgac tttttatgcg aaatatttta taattgaatt gtgccttgga gacttaaagc
    10441 atcgtaagga ttccattatt ttttatttaa agttatttat tgctaataat tgaagtttaa
    10501 atagaaatac aaaggagaac aagaaatgga agatttcgta gaaaaatatg cgaagtattt
    10561 aagagatatt aaaggcgcat ctgaaaatac gattctttcc tatcaaaggg atttaaaaca
    10621 atttatttca tttctcaaaa aggtaggaat caaagaaatt cagaaagtga acagaacaaa
    10681 tattgtcgca tatttattgg agcttcaaaa aaaaggaagg gcaccatcga cagtttctcg
    10741 aaatattgtt tccattcgtt ctttttttca atttttgtta aaaatgcata ttgttgatga
    10801 agatcctacg gaaaatattg aatcaccaaa acaagaaaaa aagcttccgg aggtattgac
    10861 catcgaagaa gtggatctct tactcagaca gccgaatgaa aatgatttaa aaggaatccg
    10921 ggataaggca atgcttgagc ttttatatgc taccggtata agggtttctg aattaatttc
    10981 attacaaaag agcgatgtga atttgacttt agaatacata aaatgtaccg accatgaaga
    11041 ttcaaaagaa agaattattc ctttaggagc atcagctctc agatccttac gtaattatat
    11101 ggataaagca agatttgcaa tgataaagga ttccaatgaa aagtatttat ttgttaattg
    11161 tagtgggact cctatgacga ggcagggttt ttggaaactt ataaaagtat atgctaagaa
    11221 agcaaaaata aataagacga ttacgcctca tatgttaaga cattcttttg cagcacatat
    11281 ggtagccaat ggggctgatt tacaatcggt tcaggaaatg cttggtcatt cagatatttc
    11341 tacaacacaa gtctatgcac aattaaataa aaataaactt aaagaggttt atactaaagc
    11401 gcatccaagg gcataaaaat gccctttttt ctattcatgg ctacataatt tataaaaaag
    11461 ctgataaaat aatgatgaaa taactttaaa tcaatttaag ggtataataa ttagaagaat
    11521 gattttaaag gtggtattga ttatgataaa aagagtgatt tggattatct tagacagtgt
    11581 gggaatggga gcaatgcctg atgcagataa atatggggac gcaggcagta atacaattgc
    11641 caatgtttat aaaaaatcag gaggtcttca gattcctcat ttgattcagc taggtttggg
    11701 aaatattgag gggatgaagg aattacccaa aattaacgag ccagtaggtt gttatgccag
    11761 attggctgaa aaatctaacg gaaaggatac cataactggt cactgggaaa tggcagggat
    11821 tattacggaa agaccttttc caacttatcc tgatggcttt cctaaagaag ttatagaggc
    11881 ttttgaagaa aaagcaggag taaaaacttt aggcaataaa gtggcttccg gaactgaaat
    11941 tattgatgag cttggagaag aacacatgag aacaggctat cctattgttt atacttctgc
    12001 agacagtgtt tttcaaattg cagcccatga agaagtcatt cctattgaaa gattgtatga
    12061 aatctgttct attgcaagag agcttttaac tggagagcat caggtggcaa gagtgatagc
    12121 cagacctttt attggagaaa aaaagggttc ttttataaga acagccaaca gaagggatta
    12181 tgcaatattg cctcctcatg aaaccatact ggataaaatt aaagcaaaga atatggatgt
    12241 tattgctatt ggaaaaatta acgatatttt ctgtggacaa ggaacgacag aacatcttca
    12301 tactcaggac aatatggatg gtgttgataa gacgatagaa tttataaaaa aagataatag
    12361 gggtttaatc tttactaatt tagttgattt tgattccaaa tggggacacc gcaataatta
    12421 tgaagcttat ggaaaaggat tggaagactt tgacagcagg cttcctgaaa tcattgctgc
    12481 tatgaaagat ggggatgtat taatgataaa ttcagatcat ggctgcgatc cgacctttcc
    12541 tggtactgac catacaagag aatatatccc atttgtagct tatggtaaag gactgaaaca
    12601 aaatgtggat ttaaaaacaa gggattcttt tgcagatatt gggcaaacca taggggaatt
    12661 gctgggaaca gaaaaaatta aaaacggaat aagttttgca aaagagatta ttttatagct
    12721 taatatatct tttaatagca atgacttctt caggataatc gggttatata atatttttta
    12781 atagagatag aagaaggtga aatcatgaga atgtatgatt taattatgaa aaaaagaaat
    12841 ggcggggtat taagtaaaga agaaattaat tttatcgtca aaggatatgt ggagggtagt
    12901 atcccggatt atcaagtctc agctttttta atggctgttt attttaaagg aatgaataaa
    12961 gaagaaactt caaatcttac atgggctttt atccactctg gggatatcat cgatttatcg
    13021 cctattcaag gcataaaagt tgataaacat tccacaggag gcgtagggga taaaataagt
    13081 ttaatcgtta ttcctttagt agcttcttta ggaattccaa ttgccaaaat gagtggcaga
    13141 ggtttaggac atacaggggg gaccattgat aaattagaat ctataaaggg ctttagaaca
    13201 caaatggata tcgatgaatt tattcataat gttaatactt ataaaatggc tatcgtagga
    13261 cagacggcta atttgactcc tgcagataag aaaatttatg ccttaagaga tgtaacggct
    13321 acggtagata gtattcctct tattgccagt tccattatga gtaaaaaaat tgcagcggga
    13381 tctgatgcta ttgttttaga tgtaaaagta ggttccggtg cctttatgaa aaatttagaa
    13441 gatgccaggg agttggcaca gaccatggtt gatattggta agtcattaaa tcgcagggtg
    13501 gtagcggtta ttaccaatat ggatcagcct ttaggtcatg aagttggaaa tgctaatgaa
    13561 gtgaaagaag ccatagaagt attaaaaaat aatggtgcgg aagatgaaaa aacggttgct
    13621 ttaaccattg cttcttatat ggcagtttta ggtggtgctt ataataattt tgaagaggct
    13681 tatatgaatt tggaaagaaa aattgaaacg ggtgaagcct tagaaaaatt taaagaattt
    13741 atcaggattc aaggaggaga tgaagaagtt gcagagaatc ctgaaaaatt gcctgaggct
    13801 aaatatcata ttgaaataaa ggcccaatca gagggttatg tgaatgaaat caatgcggaa
    13861 atgataggaa atgctgcgat gcttttggga gccggccgta aaacgaaaga ggatcagatt
    13921 gattatagcg caggaattag tttagttaag aaaattggta ataaggttat gaccggagaa
    13981 accattgcta ttttacatac caataagaaa gaaattaagg aagccatgga tttagtaagg
    14041 gatgcttaca aaatttcaga aaatcagcct gcaaaagtta aatatattta tgatgtcatt
    14101 atgtagaacg gaaagaattt catatattgc tttctggtgc atattttatc aaaaatggtc
    14161 taacatatta ttagacaaaa aattgcacta ggaggcataa atatgaaaaa aagactatca
    14221 atgatgctcg tttttatttt gtttttaaat atattttctt ttgattttgt gaaaggtgaa
    14281 gaaaccaata aagatattac tttggaaata gaatctcctt cggcagtatt gatggaagca
    14341 aaatctggta aagtattata tgaaaaaaac atgaatgaga aacgttttcc tgccagtact
    14401 acaaagatta tgaccttact tttgatttat gaggcagttg aaaaaggaca aatatcttgg
    14461 gatgataagg ttgtggtcag tgagcatgca gcaagcatgg gcggttctca ggtgtttttg
    14521 gagcccaatg aggaacagac ggtagcgacc atgactaaat gtattgctgt tgcttctgca
    14581 aatgatgcat ctgtagctat ggctgagcat attgcaggaa gtgaagaaga atttgttaaa
    14641 ctgatgaatg aaaaggcaaa agaattaggg atgaaagata cccattttgt taatgcaagt
    14701 ggtctgcatg atgctgacca tgtgacttca gcatatgata ttgccctggt ctccagagct
    14761 cttattacaa agtatcctga gattcataaa ttaacaacca tatggcagga ccatattatt
    14821 cataagacaa gacgtgggga agaagaattt ggtttaacca atacaaataa tttaattaaa
    14881 tggtatccgg gggccaatgg acttaagaca ggatttaccc ctgaatccat gtattgtttg
    14941 tcaggaacgg cagaaagaga tggattaagt ttgattgctg ttgttatggg tgcaaaaagt
    15001 aaacaggcaa gaaattcaga agtagccaag atgctcaatt atggctttgc gaattttaca
    15061 attattaaag gacagcctgc aggaaccgtt gtaggcactg tggaagttgc taaaggcaat
    15121 cctgatactg tagagggtgt tattaaaaaa gaagtgcagc ttttagtgaa taaaaaactg
    15181 gcaaatgaaa aaatagaatt taaattagaa ctgccggaat ctattcaggc acctgttaca
    15241 aaaggcatga aaattggaga aattgtatat atttttaaag gaaaagaaat tgcccgttct
    15301 gatattgttg cagcaactga tgtaaaaaaa gctggtttca aggaaatggt aaattcatta
    15361 ttaaagaatt ggttttagtg catttaagca cctgggattt ttctcaggtg cttaaattct
    15421 attgaacttt ttatttatta tgtttacaat gtagatagtc ttcatttgat ggattttaaa
    15481 taatacttaa atgaaaaaca gatgattaaa tttgtttttt aaggaggaca aagtgtggag
    15541 aatccgctaa tattactgct tgtaaaagta cctggaattg ttttcgggtt tacatttcat
    15601 gaatttgccc atggtttagt tgcatattta tttggagata ataccccaaa aaaagaaggc
    15661 agactaaccc ttaatcccct tgcccatatg gatattatag gacttataat gattttttat
    15721 atgaattttg gctgggctaa accggtgcat accaatcctt ctaattttaa aaagagaaag
    15781 ctggcaaccc tattggtttc tttagcaggg tgcgtggcta atatactttt ggcagttctt
    15841 tttgctggct tattaaagct tttatttgtt tttaatgttg atacagaaaa attttctttg
    15901 ctttatataa gtgtttatta tgcaatcacg attaatcttg tattaggtat ttttaacctg
    15961 ataccattac cgcccctgga tggttcaaag attttattta atttaatttc accaagaagc
    16021 tattttaaaa ttataagata tgaatacata ttacaaattt tactgattgt attggtagta
    16081 acaggtataa tacctgcctt agttaatcct gtcattgatt attgccttgt attattagaa
    16141 atgctatata aaataaaact gatttaatca attataaata tgaagagggg tagttctttg
    16201 agtattactg taaaattaga agtgtttgaa ggaccctttg atttactttt tcatttgatt
    16261 gaaaaaaata aaattaatat ctacgatata ccgattgctt tgattaccga tcagtatttt
    16321 gaatatatca attccctgga gtatagggat atggaaaata tgagtgaatt tttagtgatg
    16381 gcagcaactt tattggagat aaaatctaag atgcttcttc cttcacttaa gtcggaagag
    16441 gaagaagaaa aggatccgag agaggaatta gttaataaac tattggaata taaaaaaatg
    16501 aaatatattt cccaggaatt aaaagaaagg caggaagaag ctcaaaaagt tttttttaaa
    16561 gagtcagttt tacctccgca aataaaagct cttttgctgg aaaatgatga atcagatatc
    16621 agtgaagttt tggaaggggt tagtttaaaa caaatctttt tggtttttca ggatttatta
    16681 aagagaaatg aaagaaaaac agatcccata agaagtaact ttaaatcagt gaaaagagat
    16741 atttataaag tagaagataa gattacatat atcctggatt tattatattt atcaccagtc
    16801 ctttctttta aagaattatt cagcgataac tgcaacaaaa tggaaatcat tgttactttt
    16861 ttagctttac ttgaattaat acgacataaa aaaattaaaa ttaaacaaga acgcccattt
    16921 ggagatatct taattactac gaaagatagg gtggatgccg atgaaactgg agcaaactga
    16981 agcagtgatt gaagcaattt tatttgtttc aggggaagcg gttagcttaa ataagctttc
    17041 cgatattatt aatgaagatt tagaaaagac aaaagtaatt gttgagcatt taaagaaaaa
    17101 gtacgaagag gaagccaggg gaatgaggat tattgagatt aatgaagctt atcaaatgtg
    17161 tacaagccct gaactctttg agtatattaa agaattttat aagaatccta aaaaattcac
    17221 tatgacacag gcagctttag aaactttggc tatcatcgca taccgtcagc cggtgacaag
    17281 agctcaggta gaagaaataa gaggtgtcaa ttcggatcac attatcaata aattaattga
    17341 atataatttg gtttgtgaac ttggcaggat ggatgctccc ggaaagccca tattatttgg
    17401 aacaactgag gacttcttaa gatattttgg ctttaaaagc attgaggagc ttccacaact
    17461 cgaagaagaa ttattaaagc aaattgaaaa tgaagtcaat gatgaagtaa agcaaatagg
    17521 cctttttgat gaccaagaga attaacattc ttttggtcat tttttgtatt ttttattact
    17581 tttgcgggaa aaatataata attagaatta tatgcttaaa taaagcttat gagtaggtga
    17641 agtcttgaag tttgtcattc attatgataa aacagcggat gcacatcgct tttatttatc
    17701 aggataccta ttcaattatg ttaagatttt aaatattgat ttgaaagatt attttattaa
    17761 tttattaagt cattttctaa aggaagatga taacaattca aatattacaa aatcatacat
    17821 aaactatgct caagcagcaa aaattatatg gtgtatatgg aaaaaatcaa aaaagcatat
    17881 ccagattcct tttataggca tagagataga aattgctaat gaaaatccag ctttgacagc
    17941 tttattattt ggaatcatga atgcctgcat accagcagct atttatttac tggatgaaaa
    18001 aataagtata agtgactatc ggattcttat tgtgcccaat tttgatttag agaaaatgaa
    18061 tataaaaata ttagctgaaa taaaaatgga tctgatttta ttaatcctga tctatattca
    18121 aataaagagg aggattaaaa aaaatgacaa attccagtca gcaacatccg attgaatctt
    18181 tgttaaaagc agcaatggaa aatataaaag aaatgataga tgttaatact gtagtgggta
    18241 atccagtaga aaattcagaa ggaatcatga ttttaccaat tactaaggct acttttggat
    18301 ttgcggcagg aggagccgag tatggaaaat tgaaagaaaa tacatcagaa gatgaaagta
    18361 aggcatcttc taaaagatat ccctttggag gaggaagcgg ggctggaatc agtattcaac
    18421 ccgtagcctt tatggttgtt gaaaaaaatc atgtacaaat tatgcatatc catcataatt
    18481 tcggtacgat ttctaaaatc ttagaggaag gtccaaattt cctgaccaaa cttgaaaatt
    18541 gcttaaattt aagaaataag aaaaagatta aagcagaaga aggtacaaaa gaaaagggcg
    18601 gaagagtgat ttctaaaatt gtggaaattg aagagactta acttaaaacg aaggtttgcg
    18661 tcgataagaa agaatttttc gaaaaggtga taatgatgaa gaaaagatta ttagcaatga
    18721 tagtcattat aactttaatc ttcaatatat atccgttaag cttatatgga agtgaaataa
    18781 aagagccggt gattgaagcc aaagcagcaa ttttaatgga tgcaaaaacg ggcaggatat
    18841 tatgggaaaa aaatgctcat gagcctttgg ctatggccag tacaaccaaa attatgactt
    18901 gtattttagc cttagaaaaa ggcaaactga cggatgaagt agtggtcagc aaaaaagcag
    18961 ccagtgcacc aagggtcaag atgtatttac aagcaggaga aaaacagtta ttaggagact
    19021 tactgtatgc ccttatgctt cagtcttaca atgatgcagc tgttgccatt gcagagcata
    19081 tcagtggttc tgttgaggaa ttttgtgaag agatgacaaa aaaagcaagg gctataggag
    19141 caaaaaacac tgtttttaaa acacctaacg gtttagatgc ccagggtcac caatccacgg
    19201 cttatgattt agctttgatt acaaaatatg caatgcaaaa tcctaaattt gtagaaatca
    19261 ttaatactaa agaatatatc attccacaaa agggaaatta taaatcttat catcttagaa
    19321 atgcaaaccg ctttttaaat gaatttcctg gagctaatgg cgtaaagacg ggctatacgg
    19381 ggaaagctgg ttattgcttt gtaggagcag ccagacaagg agagatgcag ttgatttcgg
    19441 ttgttttagc cagtggctgg gggcccagag gaaaggagca aaaatggatt gatactaaaa
    19501 ggcttatgaa atatggtttt gattattata agatagagac cttattggaa aaggataaag
    19561 ttttaaaaac aatttcggta gaaaaatcaa aagatgaaca ggtttcttta tatacggacc
    19621 gttgtataga atatgccata agggatgaag aaaaatcaaa aataagagtt gaattagagc
    19681 ttccaggtat gattgaagca cctgtagaaa aaggagaaaa acttggcgaa gccagggttt
    19741 atataggcga tgaattttgt ggaagtgtta atttgttggc agacagaagc attgaacggc
    19801 atgattttcc caactgttta aaaaaagtac tcaacaactg gttaaaaatc attcattaat
    19861 gaataagctt ttataataaa gatgaaagga tttcctttca tctttattat aatattgaaa
    19921 aattttatat tttttaagaa caaattttaa aaaatataat tttgctttat aattataata
    19981 ataaaaattt tacagagcag attttttaaa gagggagatt ttatggaaat gcgtctgcag
    20041 aaatttttgg ctaatgctgg gatagcttcc agaaggaagg ctgaagagta catactggaa
    20101 gggaaaatta aagtcaatgg ccaagtggtt actgaattgg gtacaaagat taatccggac
    20161 aaagatctgg tttattataa taaccaaatc gtaagcctgc caaaagaaaa aatatatctt
    20221 ttgctacaca aaccagaaaa atatgtgaca acggttaggg atcagtttaa cagaccgacc
    20281 gttttagatt tgctgccccc ggctttaaag gctgtccatc cagtaggacg cctggattat
    20341 aacagttccg gccttcttct ccttaccaat gacggggatt ttacctataa gattactcat
    20401 ccaaaacatc atatcagaaa aatatatatt acaaaagtca aggggattcc tgccgaagct
    20461 tctttagata aattaagaaa aggattggtg attgaccatt ataaaacatc tcctgccaaa
    20521 gtaaagcttt tgggcaaatc aaaaacagag gcggtacttg aaattacaat ttatgagggt
    20581 agaaacaggc aggttagaaa aatgtgtgaa gcgataggac atcctgttat ttctcttaaa
    20641 agaattgcta tcggaaaggt ttttttaggg aatttgccag taggggagta cagattttta
    20701 acaaagcaag aaattgatta ttttatgagc ttataatttt aaagtcgtta tattatttta
    20761 agtaggtgat aacttgttta tagatttgaa attaacggat atggttcatg aaatactcaa
    20821 agggaaaata agagaaggag atacggttgt tgatgcgaca gtgggaaatg ggaatgatac
    20881 actttttttg gccagagcag ttggaaaaac aggaaaagtg attggttttg atattcaaaa
    20941 aaaggctctt catcaaactc aaaataaatt gattcaggaa gctttggcag acagagtttt
    21001 attaatacat gattcgcata gtaatatgga tcaatatatt aaagaaccaa ttcatggagc
    21061 aatgtttaat ttaggctatc tcccgcaagg ggataaaaat atcattacaa aagctgatac
    21121 aactattgaa gctttgaaaa aagtctgttc cttattatta acgggagctt ttattacaat
    21181 tattagctat tggggacatc agggaggtct tgaagaaaaa gaagcagttg aagatttttt
    21241 gacaaaactg gaagcaaaaa agtatatttg tgccaagttt aattatctta atagggaagg
    21301 atgtccacca attatatatt ttctgcaaag gttgtagatt ctatattttt ttaatatttt
    21361 atcttcataa attgcttgca ccgattatga gaatcatgta gaatgaaatt tggtgattgt
    21421 tgcgtttttg acagagttta taatttataa atggagggtt ataaatgagc acattatcta
    21481 aagttaatga actcaattca cttcgctcaa aaattatgga gggcggaggg gaagctaaaa
    21541 taaaaaagct tcacgatgaa ggcaggttaa ctgcaagaga acgcattgaa aaatttttgg
    21601 acgaaaacag ttttgtagaa atgggtttat ttgtcaggca ccgcagtaca gactttaaca
    21661 tgagccaaaa agaaaccccc gcagatggtg tagtgactgg atatggtacg gttgaaggac
    21721 gcctggttta tgtctatagc caggatgcta cagtccttgg aggttctgta ggggaaatgc
    21781 atgcaaaaaa aattatcaat ttatatgacc aggcgcttaa aatgggtgca cctattgttg
    21841 cttttttaga ttcttctggt gtgagattac aagaaagtgt tgatgcttta gatggttatg
    21901 gcagcttatt tgtaaaacag actatggctt ctggagtgat tccacaaatt acagttgtaa
    21961 tgggagattg tattggaggt gcagctttta atacggggct ttctgatttt acttttatgg
    22021 tttccaaaaa gggaagatta tttgttaaca gccctaatac catagaaggt cctgaaagta
    22081 aaaaagcaaa ttttgaaagt attggaagtg caacgatgca tagtgaaaag agtggacttg
    22141 tgcatttcca gtatgatgat gaacttgaat gtatcaataa tgtgagaaaa ctggtttctt
    22201 atcttccttc taataattta gaagaagcgc cttattataa tgtaaccgat gacttaaatc
    22261 gtgtcgattc tgttttaaat tctattgttc ctgatgattt aaatgcagcc tttgatataa
    22321 aaacagttat ttgtgcttta gctgatgaca gagagtttat ggaggtaaag gcaaaatacg
    22381 ctaaaaacct tgtaactggt tttctacgct taaatgggac aactgttgga attctggcca
    22441 atcaatcttt agaaaatgaa ggtttattgg atatcgatgc atctaaaaag ggtgcagaat
    22501 ttattaattt ctgtgatgct tttaatattc ctatcgtttc aattactgat gctggtggtt
    22561 ttaaagcttc tttagaagaa gagcaaagag gcattgttaa atacgtaagt aaattaattt
    22621 atgcttttac caatgcaacg gttcctaaag taaatatcat tgtaaggaaa gcttttggaa
    22681 cagcttatat tgccatgaac agtaaacata ttggtgcgga tatggttttt gcatggccta
    22741 gtgcacaaat ttccgtaatg aatcctgaag gagcagtcaa cattatgtat gcggatgaat
    22801 taaaagctgc ttctgatccg gcagccctca gggcagaaaa ggttaaagag ttttcagaga
    22861 tgcagtccag tccatacaca gtggcatcca ggggatatat tgacgatatt attgagccag
    22921 cagctacaag aaaacgtgtt attgctgctc tggaaatgct ttattcaaaa agagaagaaa
    22981 gacctgctaa aaagcatggt agcctataaa gtataatcat ataacgagca aatgaatgga
    23041 ggttaatatg ggcgatctaa ctcatggatt gatggtaaca gtcattggaa tgggtataac
    23101 atttattgtt ttgcttgttc tatcttatgt tttagattta ttcagaattc tttcaaaagg
    23161 gccttcctca aaaaaagcag atgctgagga gaaggcagaa aagataaaac cagaacctgt
    23221 tgaaaacatt tctattgcag aagaaacaaa ttcaaaaaat gatttggaat tggtggcagt
    23281 tatcactgcg gctatagcag cgagtttaaa tacgacaagt gaccaattgg tcgttcgttc
    23341 ttttagaaga attggaaaca gatcatctca atggaacaat ttattttatt aatcaatgat
    23401 ggaggaggaa ataaaatgag aagatttcaa gtaactgtaa atggaaatat atatgaggtt
    23461 gaagtggaag aattgcaagg agggcaagtt gcagcaccag cagcaccaac accagtagct
    23521 gctccaagtc cggcggttaa agtggaagcg cctaaagctg ctccacagcc agctccagga
    23581 ggaacagaag gtgcacataa ggtaactagt ccaatgccag gaacgatttt agatattaaa
    23641 gtaaacattg gagatagtgt aaaagtaggc gatacagttg caattcttga agcaatgaaa
    23701 atggaaaatg aaattgtagc aacagctgat gggaaaattg cttccattaa tgtttcaaaa
    23761 ggtacacaag ttaatacagg tgatttaatc gtaactatag gctaaaaatc ctggccatta
    23821 tttaaaaagg agtgaataaa gcaaatgagt tttattgaaa tgctgcttga aaccatgagg
    23881 gatcttttgg cagaaagtgg ttttgcgggc ttaactttca aacatgttat tatgatcgtt
    23941 atatcactgg gactaatgta tctggcaatt aaaaaagaat atgaaccttt attactttta
    24001 cccatttcgt ttggaatgct ccttactaat cttcctgcag caggcttgat ggcggagccg
    24061 gcaaatggtg aagttggagg tattctttat tacttatatc aaggtgtaaa attaggtatt
    24121 ttcccacccc ttattttctt aggcgtagga gccatgacag attttggtcc tttgattgct
    24181 aatccacgga gtattttgtt aggggctgca gcgcagctcg gtatattttt tgctttctta
    24241 ggagcgttag gtttaaaata tattgtacct tttattaatt ttacaggggc agaagcagct
    24301 agtattggaa taatcggtgg tgctgatgga cctacagcga tttatttaac atccaggctc
    24361 gcaccgcact tattaggacc tattgcagta gcagcttatt cttacatggc attggttccc
    24421 attatccaac cacctataat gagggcgctt acgaccaagg aagaaagaat ggtaaaaatg
    24481 gaacaattaa gaccagtttc taaaaccgaa aaaatacttt tcccaattat cgtaacaatc
    24541 ttagtttcat tgattttacc ttctgctgca cctcttgtag gaatgttgat gctgggtaat
    24601 ctttttagag aaagtggtgt tgtaggtaaa ttagttgata cggcttccaa tgctttaatg
    24661 tatattatta caattttcct gggaaccact gttggagcaa cagccagcgc tgaaaacttt
    24721 ttagatccaa agactttagg aattatcgtt ttgggtcttt tagctttttc cgtgggaaca
    24781 gcaggtggtg ttttgtttgg caaaattatg tatagaactt ctggaggaaa ggtaaatcct
    24841 cttataggtt ctgcaggggt atccgctgta ccaatggctg cccgggtatc acaaaaagtt
    24901 ggtaaagaag aaaacccaac aaatttctta cttatgcatg ctatgggacc taatgtggca
    24961 ggagttattg gttctgctgt tgccgcaggt gtattattgt ctatttttgg agcataatac
    25021 tgttaatata cggactttta tgaataggag gtagaagaat gtcagataaa aataatgttc
    25081 ggccagttaa gattacagat acaacttttc gggatgcgca tcagtcttta atagcaacga
    25141 gaatgactac agaagatatg cttccgattg ctgaaaaaat ggatcaggtg ggttttcatt
    25201 ccatggaagt atggggtggg gcaacatttg attcttgcct gcgcttttta aaagaagatc
    25261 catgggaacg attaagaaaa ttaagagcaa ccatcaaaaa tacaaaatta caaatgttac
    25321 ttagagggca aaatttgtta ggttaccgcc attatgcgga tgatgtggta gaatattttg
    25381 ttcaaaaaag tattgccaat ggaatagata ttatcaggat atttgatgct ttaaatgata
    25441 ccagaaattt agagactgct attaaggcag ccaataaaga aaaaggtcat gttcaggtag
    25501 caatttctta taccttaagt gaagtgcata ccttagatta ttatgttaaa ttagctaaag
    25561 aattagaaag catgggtgct cattccttat gtattaagga tatggcaggt ttgctactgc
    25621 cttatcaggc ttatgaactg gtttctgctt taaagaaagc tgttaagatt cctattcaac
    25681 ttcatggtca ttataccagt ggggttgttg ccatgtctta tcttaaaggt attgaagcag
    25741 gagcagatgt tgttgatact gcaatgtcac cattttcaat gggaacttcc caacctgcaa
    25801 cagaagttat ggtagaaacc cttaaaggaa ctccttatga tactggttta aatcaagaat
    25861 tattggctga aattgcagat tatttcaggc cacttcgcga aaaagctatg gaagaaggtc
    25921 tgttgaatcc aaaagtttta ggtgtagata ttaaaactct tttatatcag gtacctggtg
    25981 gaatgctttc caatttagta tcacaactta aaacacaaaa tgctgaagac cgtttctacg
    26041 aagttttgga agaaattcca agagtaaggg cggattttgg atatcctcca ttagtaaccc
    26101 caacaagcca aattgtagga actcaggcag tattaaatgt attaatgaat gaacgctata
    26161 aaatggttac taaagaatcc aaagccctgg ttaaagggga atacggcaga acgccagttc
    26221 caatttcgga agaaattgtt aagaaggtaa tagggaatga aaaaccgatt acttgcaggc
    26281 cagctgattt aatagagcct gaattagaaa aaattgaatc agaaatgaaa caatacaaag
    26341 aacaggacga agatgtactg acctatgctt tattccctca agtcgctgaa gaattcttta
    26401 aatatcgtca ggcacaaaaa acaaaagttg atgttaattt agcagattta gaaaataagg
    26461 tgtatccagt ataataactg ttaatcattt catcgattta gaaagaatag attgagtcat
    26521 ttaaatctaa tagaagaaag tttttataaa gtgtgcaatt ttgcacactt ttttttaata
    26581 ttatttgtaa aataaatatt tttatatata ataatgatat gactaaagca ttcagggtag
    26641 atttaatagt atcaaaattt agaaatgtga gggactagta tatgtataat gggaatgatt
    26701 tgattcagcg gattcataaa aatttaccca agttaagtaa agggcaaaag ctgattgcca
    26761 attatatttt aaaccattat gaaaaagcgg tttttttaac agcggcgaaa ttaggcgcgg
    26821 tggtaggtgt tagtgaatca accgttgttc gctttgcgaa tgtgttgggt tatgatggtt
    26881 atccaaaact tcagagagct ttagaagaac tggttaaaaa taaattgact tctgtccaga
    26941 ggatggaagt ggcctacgat cgactggatc aaaaaagtgt tttaaaatac gttttacaat
    27001 cggatgcaga taaaatcaaa catactctgg aagaaattga tgaaacatct tttgacaata
    27061 cagtaaatgc aattttaaat gctcgtaaga tctatatttt aggtgtaaga agttcagcag
    27121 ccctggcaag ctttctggga ttttatttta atcttttatt tgataacgta aaattaatcc
    27181 ataccaatag cgtcagtgaa atgtttgaac aaattcataa gatgaattcg gatgacgtgg
    27241 tgattggaat cagctttcca aggtattcaa aaaggacaat aaaagcaatg gaatttgcta
    27301 aatctcaaaa ggctacagtt attgccatta cagacagtcc tttatctcct ttggtccgtt
    27361 atgcggatta tagtctcctt gccagaagtg atatggcttc ttttgtggat tctttagttg
    27421 caccaatgag tgtacttaat gccttaatcg tcgccattag tttaaagaaa aaagatgaaa
    27481 tttctgagac cttaagtact ttagaaaaaa tttggacaga atatcaagtg tatgataatg
    27541 aagatgaaga cttgaattta acctattctt taaatagaag acaaggtggt taagatgctt
    27601 aggctttatt tggtaaggca tggagagaca aaatggaata tagaaaataa atatcaggga
    27661 gcttcagata ttcctttaag cccggtgggt attgagcagg cacaagctct tgccaggaaa
    27721 ataaaagctt tcaaaattga taagatttat tcatcggatt tgtcaagggc ttatgagacg
    27781 gccaaaatga tagcaaaaga aaaaaatctt gaaatagaat gcatgcctgg cttaagggaa
    27841 atcagctttg gtgaatggga aggctatacc attgctcaat tgaaagaact ttatggtgac
    27901 agttataaaa aatttttttt agaaccccat aaatatcctt ttcctggaga ggggagtatg
    27961 gtggcggttc aaacaagaat tacaaaagca gtttctgaaa ttgtaaaaaa acatcccagg
    28021 ggagatattc ttattgtctc gcatggaggt gttttaaaag tattaattat gtctttatta
    28081 aaaatcgact tatccttttt taaaagtttc tggttaggca atacttcttt atcaatcatt
    28141 aaaaaaacag atgatgaccg ttgggtctta agtcttctca atgatcttag tcatcttgaa
    28201 gaactaaact attgataaga ttttaccatt aaaaatataa tatgaggagt tattgttatg
    28261 tctgtatgtg caatcagagg cgcaattaca attgaagaga atacaaaaga aaatatcctt
    28321 gaaaacacta gtcttttatt aaaagaaata attcataaaa ataatattaa tattgatgat
    28381 attatttcaa taacctttac agcaacagat gatattgatg cggcttatcc ggcagtagct
    28441 gcgagaaatt taaatattac aaaagctgcc ttattatgct ttcaggaaat gaaggtgatt
    28501 gacagtttaa aaatgtgtat cagggtgatg cttttggtga gaagcagcaa aagccaggac
    28561 caggttcatc atgtctattt aaaaaatgcc gttgtattaa gaccagattt gatccattaa
    28621 aaattcagga ggttattatg gaacgttttg ctattgctat tgatggcccg gcaggagctg
    28681 ggaaaagtac aattgctaag ctgcttgcaa agcgcttaaa tattatttat gtagatacag
    28741 gagctatgta cagagcagtt gctttatact gtattgaaaa tgcctttgat tataacaaaa
    28801 aagaagatgt tgaaagagca ttagagaata ttgatattaa aattgtttat caggatgaca
    28861 atcaaaggat attgttaaat aaccgggatg taacagattt gattcggaca ggggaagtgt
    28921 ccaaaggttc ttcagccgtt gctacaattt atgcggtacg gataaaaatg gttgagttgc
    28981 aaagaaatat tgcaaaagag aattctgttg taatggatgg aagggatatt gggacctatg
    29041 tattgcctga tgccaatctt aaaatattcc tgacagccag tgttgaggag cgggcaagaa
    29101 gaagatataa tgaattgatg gaaaaaggaa tagaatgcaa tttagagcaa atcaaaaaag
    29161 aaatcgaaca aagagataaa aatgattcaa gcagagagtt tgctccttta agaaaagcag
    29221 atgatgcgat tgaaatcgat acgacgaatc taacgattga agaagttgta gagaagatta
    29281 ttgctttatt atgaatgaaa atgacggata agggatgatt tagagttgaa taatttattg
    29341 tctaatgata aaattggtat agtcacagaa gctaaaaggt ttctgtttgc aaattattaa
    29401 ggaggttttt acaacaatga atgatttaga aaaccataat aacgaaagtt ttgaagcatt
    29461 acttaatgaa tcttttgtta tattaagaag cggtgaaatc gtaaaaggta ctgtgattaa
    29521 tgtaaccgat acagaagtaa tggttaatct tggatataag tccgatggta ttatttctaa
    29581 agctgaattt tctaatgatc ctcatattaa ccttaagaat gccgttaaag tgggagatga
    29641 gatagaagta tatatattaa gagtcaatga tggtgaaggc attatagaat tatctaagaa
    29701 aagagtagat attcaaaagg gatgggatat ccttcaaaaa gcttatgaag cagaaagtat
    29761 tttaacaggt agagtagttg aagcggttaa aggcggcatt attgttctta atcatgaagt
    29821 acggatattt atcccagctt ctttgttagg tgcccgttac attcaggatt taaatcaatt
    29881 tcttggacaa accattcatt ttaaaatagt agaatttgat aaaaaaagga gaaagataat
    29941 aggagaccgt aaacaagtac tggtagaaga acgtaaaata aaacgggaac aattattaaa
    30001 tagtttagaa gtcggagatc tggtgaccgg cagggttagt aatataacag ggtttggtgc
    30061 ttttgtagat ttaggcggca tagatggttt aattcatagt tctgaattat catggaaaag
    30121 gattagtaag ccttcagata ttctgaagat tggtcaggaa gttaaagcca gagtgattca
    30181 aattgataaa gaaaatgaaa aaatttctct ttctttaaaa aaagaagaag aaaatccctg
    30241 gactaatgta gaacaaaaat atcctgttgg ttcagtcgta aaaggaaaag tcgtaaggat
    30301 ggttcctttt ggggcatttg tggaactgga agatggttta gatggtttga ttcatatttc
    30361 tcagattgcc aataagcatg tggcaaatgc tgaagaagaa cttcaacttg gtcaaatcat
    30421 tgaagcaaaa gtgatggaag tggatgaaaa acaaaagaaa attagtttaa gtattaaaga
    30481 aattaagaaa gaaactgaac aagataatga gaaagaaaat gaactttccc aagaataaat
    30541 gcggctattg ccgcatttat tagcttatca ttttttgcgg aggtataaaa tgattgtaac
    30601 ttatgaagat tttaaaaaag agatctatca tatgtctggt attgatttga actgttataa
    30661 ggaacggcaa atgaaaaggc ggatagatgc tttaattaag aaaaataatt ataatgacta
    30721 tcattcttat gtactggcat taaaagataa taaagaacta tacaatgaat ttatcaatta
    30781 tcttaccata aacgtctctc aatttttcag gaacccttca caatgggaag tgttagaaaa
    30841 agaaatcatt ccttacttgc taaaatcctc atctgattta aaaatatgga gcgctgcctg
    30901 ttcaacaggc gatgaacctt attctttagt aatgttatta tctaaattta ttcccctttc
    30961 aaaaattaaa atcttagcga cggatattga taaagaagcc ctcgaaaagg ccaagatggg
    31021 tctttacaac gataaaagca tagaaggaat gccgaaggag ttttttaata aattttttaa
    31081 gcctgtagga gagttttttc agatttctga agaagtaaaa aagtgtgtta cctttgagca
    31141 tcacaattta ttaagtgata gatatcctca aaattgtgat ttaattgtat gtcgaaatgt
    31201 tttgatttat ttcacagaag aagcaaaaaa taatatttat aaaaatttta ataagtcctt
    31261 aaagatggac gctattttat ttgtcgggag tactgagcaa attattatgt ctcatcgtta
    31321 tggttttacg cctgttaagt ccttttttta taaaaaaata gcagaattat gatttttagc
    31381 tgagttggtg aaacacaatg atttatatca ttatttcaat gatcatttgg ggaagtattg
    31441 gtttatttat ccgttggatt aatatgtctt ctattgaaat tgctttttta agagcgggga
    31501 ttgccgcttg ttttctcttg ttttattcgt ttttttataa aaaaatagat atgaaaaaga
    31561 taaaagaaaa tatcatttta ttaattttgg ctggcgctgc ggtagctttt aactggatta
    31621 tgctttttca ggcttaccgc tttaccagta ttgctacggc caccatttgt tattattttg
    31681 caccggtcat cgttgttttg ctttctcctt ttgtttaaaa gaaaaaatga ccctggtgaa
    31741 aattttttct atttttcttt ctatgacggg attgtttata atgatgtatt attcccctga
    31801 aaatgtggga agaggttcta ataatttatt gggaatttta tttggccttt tagcagctgt
    31861 attttatgct cttgttgtag ttcttactaa aaaaatgaag gatatagagt cttttgagat
    31921 tacctgcatt gaaatttttg ttgctgcttt ggttttattg ccttttgttt tatatcatcc
    31981 tcaactacat atcaaaaata ttcagacagg aattattgtt tgcattttag gtcttattca
    32041 tacaggtttg gcttatatac tttattttaa gggaataagg caggttaagg cacaaaacgc
    32101 agctttactg agttatattg atcctctttc ggcaatttgt tttagtacgg tgtttttagg
    32161 tgaaattcct aataaagcac aagtcattgg tgctctgttc atttttatgg cttcatttat
    32221 tcatgaaata aagataaaaa atccacaaat taagcaaaaa gcttaatttg tggatttttt
    32281 taatggtttt tatggtcgtg actgtgatgg caatgacaat tttcatcatg gtcatgatta
    32341 cagtcacagt gatgattatc aaaaaagcca taataagcca taattcttcc aatgttatcg
    32401 acaattctgg ctttattttt atcagaagac atattaactg cattgattaa gtagtccata
    32461 aattcatctg aattcatggc aagaggtata agacctaaga agttttgcat ccgtgctttt
    32521 cttaatggat ttgtcatgtt tttaaattca gaatccaggt attgaataaa tttctgtata
    32581 tatttttcaa gttccttatt gactgacaaa tctttttgat ttttagtatt gtaaactaaa
    32641 gtatccatta aacgctggtt aagatgagaa aatataagca gatggttttc aaagatattt
    32701 gacaattctt catggctatc aatttttaaa ctctcattac tgaggatatc tttgatttta
    32761 cgatttattt ttaataaatt gttttcaagg attcctatat gttgattcat taaatccggt
    32821 aatgtttcaa tcaaaatttc tttttcatgg gggtcagtat cttcgttaaa gagcgaagaa
    32881 gatgacaggt aaaaatcctt ataggccaca tgcctgttgt ataggctttc taaatcaaca
    32941 ttctgaagca tcaaaagcac tgacatacag ttaaagatgt catacaggga gaggagtaaa
    33001 tcgccaagat tcagcaaatt gtcttcgatg gattcaattt cttttaatat accttcaatt
    33061 tcatctttat taggtttttc taaagctttt tctatacaat tttcaatgga cattgcaata
    33121 tccggtaaaa agtcatgata tcctttaaca tgcttgccat taaattgttc ttttaagaat
    33181 tcaaaaatat cttttaatcg ttcttcgggt tcattaatca gtaaaaggct tttttctaca
    33241 tattcataat acttgttttt actcacccgg atagggattg aacttagtaa ctgacaaagt
    33301 actgactcta aatcatctgg actgataaat tcatttataa atacaaaaca atcttcaaag
    33361 aaaaggttga aatctaaaga ttctatatta ttatcttttg aagacagcag attgtttttg
    33421 tcatttaaaa tcagtgtggc aatattaagt tcagtatagt aagcatataa aattctatac
    33481 aatttctcta atttttcctt ataattgata atggtttcta tttttttaaa tttttcttcg
    33541 ccattttctt tgaggacatc ttcaagattt aaaaaaagat ttatagcata gtttgacatt
    33601 tcatataaat tttcgttaat atcattaata taagttaagc tttcatttat atacaaatgg
    33661 acagaatgat taagaagttt taaaaaggca aggggtacac tttctttaaa agaattattt
    33721 aaaataagct ctaaactttc tttgctatta ttggtcaaag tcattctcct ttcatctttt
    33781 gcacatcatt ataacataaa atattctata agatttactg aattttttat agttatatcc
    33841 gatatataat tatataatta tacctgagta aaaaatggta cccttaatca ggtacggagg
    33901 tgtaacatgt ataaggtatt atgtattgat gaagaaaaaa atcaagtgaa taaaattgtg
    33961 aaaatgatag tggattcagg acttgatttt acagttttaa aaacagtaag tactagaaaa
    34021 gaagcattag attttttgga gaaaaatcaa ttagatttga ttgtttcaga tatcaggctg
    34081 ccagactata atggctatca gttatttaag gatttaaagg aaagccatcc agaaattaaa
    34141 atgattcttt atacatctta caatgaatac gaatatgttc agaaagcttt agaagaaggt
    34201 ctcatagatt atattttcaa gcctgtaaaa gagcaggaat taaggaggag cttgattaga
    34261 gcaaaacaac ttcttgatga tatttcaaga agatacgaag aacaaaacag gatattggct
    34321 aagtttgatg aatctctgcc tattttgcaa gacaggtttt taaccaattt aattcatggc
    34381 tatccggaaa aagaaagtga catacataag acttttgaat attttaatat catgattcct
    34441 cctggttata cagtgatggt tttaaagata gattattatg ataagctttc attaattatg
    34501 gaagaaaaag ataagcatat gcttatattt tctgcattat ataagattga atctattttg
    34561 gaagaatata aaaatggaat agcctttatt aaccgacatg atgaaattgt cattatttta
    34621 ggaggaaagc tttcaattaa cgaatctatt acaatagggg aaaaaataag aaaatcaatt
    34681 gagacagatt taaaaattac ttcaaccatt ggaattggta agatgtataa tgaagcaaga
    34741 aaaatcaata tttcttataa gcaggctaag gcagccataa gatataatta ttatctaggc
    34801 tttaattcag taatacatat taattatgta gaacccacta acattattac ctatgaatat
    34861 cccttaaaga aggaagagta tctggtttat gagacggtta taggcaatga aaaaagggtt
    34921 ttggaactat tagaagattt gtttgctgtt ctgaaggcat gtcagcctct tccagataaa
    34981 ttattaccga agattatttt agatattatt gtatctatta accgttatgc cagtgaacag
    35041 ggtataaata ttgacagctt ttttagtgaa aacttttcta tgaaagatgt attaaagcat
    35101 aaggagcaca atgatgcttt taattattta tcaaacttac ttccagccat ttgtaaattt
    35161 attaaggaaa aaaggaaaaa gaatgaagaa gctattttag ttaaagccaa gatgtatgtt
    35221 gaagaaaatt tccttagcaa tattacactt aatcaaacag ccatgcatgt tcaaaccact
    35281 cctgaaaatt taagtgtcat tttggctgaa gcaggatgtc gttcctttta cgaatattgt
    35341 acaaagatta gaattgaaaa agctaaaagt ctgctggagg aaaccaattt aagtgatgaa
    35401 ggtattgctt taaaaattgg ttatgaagac agaaactact ttagaaaaat ttttgaacaa
    35461 atggaaggta tgacaaccga tgaatataga ttattaaaaa aataaaccga ggattctcgg
    35521 tttattggtt tttatatcaa tatttatcaa tgaaaagtcc gtcttcattt attgtttatt
    35581 attttaaagc atgataaaat aaacaagaca ttatagaaaa tttaaaataa tgtgagaaag
    35641 catagaaaat aatagattta taatggaggg caatatgaag acgacagtta atgtaacaga
    35701 gatgtatgaa attattcatt cagaacatag agatcctcac catattttag gaatgcatga
    35761 aattagaata cagagaaaaa aggccattgc cataagggcg tttattcccc aggctttgag
    35821 tataaaggtt attgaagaaa acacaaataa aaaatatgat atgggaaaaa tccatgaaag
    35881 tggctttttt gaaattgtct ttttaaataa aaaagaaaag ttcaggtacc aattggaaat
    35941 tatggattat cataatcatc aatggacgat atacgatcct tatgctttta atccgattat
    36001 atcagatttg gaccgatatc tttttaatca agggactcat tataaaattt atgaaaaatt
    36061 aggttcccat ccaatgacgg ttgatggggt aaaaggtata ttatttgcag tatgggcgcc
    36121 taatgcaaaa tgcgtcagtg tcgttggttc ttttaatgat tgggatggaa gaagacatcc
    36181 catgaggtgt ttaggggata gtggcatttg ggaattattt attccaggag ttttagaagg
    36241 ggatttatat aaatatgaaa ttaagacaaa agaaaatcag cttttattaa aaacagatcc
    36301 ttatggattt tatacagaat taagacccag tacggcttct attgtatacg atattgataa
    36361 atatcaatgg catgatgaaa aatggatgaa ggaaagggcg caaaaggatc ccttaaacca
    36421 gcctctttct atttatgagg tgcatttggg ttcgtggatg cgggtagtag aagaaaataa
    36481 ccgctttttg acctatcggg aagcagcaca taaattggtt gcctatgtaa aagatatggg
    36541 ttatacccat atacaattga tgcccattga agaacatcct tatgatggtt catggggtta
    36601 tcaggtaaca ggctattatg ccccgacaag ccgttatggt aagccggaag attttatgta
    36661 ttttgttgat tactgtcatc aaaacggtat tggcgtcatt ctggattggg ttccaggaca
    36721 ttttcctaaa gatgcacatg gtttggcccg ttttgatgga acagctttat atgaacatga
    36781 agatccaaga aaaggagagc atccagactg gggaacctta atttttaatt atggccgtta
    36841 tgaagttaaa ctatttttaa ttgccaatgc gctttattgg tttgagaaat atcatgtaga
    36901 tggcttaaga gtagatgctg tggcttctat gctttatttg gattatggaa gaagggatgg
    36961 ggactggata cctaattcct atggaggcaa tgaaaattta gaggcggttg aatttttaaa
    37021 gcatttaaat tccattgtat atcaatatca tccaggcatc atgatgattg cagaagaatc
    37081 tactgcatgg cccaatgtat caagaccaac cgatatggga ggattaggct ttggtctaaa
    37141 atggaatatg ggttggatga atgattttct aagatatatt tcaaaagatc ctatatatag
    37201 aaaatatcat cataatgatt taacctttgg actggtttat gcctatacag aaaatttcat
    37261 acttgtgctt tctcatgatg aagtggttca cggcaagcac tctatgattg gtaaaatgcc
    37321 tggagactat tggcagaagt ttgccaattt aagagtggca tatggcttta tgtacgggca
    37381 tcctggtaaa aaattattat ttatgggggg agaatttgcc cagtttgatg aatggagtga
    37441 agcaagaagt cttgactggc acctgttaca atttgataaa catcgggaaa tgcaagccta
    37501 tgtcagagat ttgaacagac tttatttaaa agaaactgcc ttttgggaat ctgatttttc
    37561 ctgggaaggc tttgaatgga ttaatggtca tgatgcggaa agtagtatta tttcttttat
    37621 aagaaaaggg aaagaaccac aaaattattt gatttttgtt tgcaacttta cgcccgttcc
    37681 ccgctatcag catagaatcg gtgttcctga aaaaggaaaa tataaagaaa tattcaatag
    37741 tgatgccagt caatatggtg gcagcaatgt tattaatgag caggaaattg tatctgaaag
    37801 tatagaatgg gatggcagag aaaacagtat aggattaaaa gtgccgcctt taggtatggt
    37861 ggttttgaaa agagtggatc tttaatagaa aaagtatacc gatgtgataa aatcgtcaat
    37921 aagactttca gaccgtcgtt aaagtccgtc atgagacgga cttttttata tggctacaga
    37981 ctctgtgaaa aacttcaatt ttttgaacca gtaaattaaa aaagtattgc ataaaattac
    38041 acaagcatgt ataattatta ataattattt caagaaagat tagtaaaagt cttactattg
    38101 ttttttgtgg gagagatgtc tatgcaaatt attgatggtg gtgtgaccag tccaaagggc
    38161 tttatggcca atggaagcca tgttggaatt aagaaaaaga aaaaagattt atccgtcatt
    38221 tatacggtat ttcctgcaaa aggcgctgcg gcttttacaa ccaatgtcgt aaaagcggct
    38281 cctgttttat ggaatcaaaa aattatccat gaaagaggaa atatccaggc aattgtgatt
    38341 aatagtggta atgccaatgc ctgtactggg gagaaaggta tgcaggatgc aatgttaatg
    38401 gcaaaaacag ctgcagacgc tttaaatctg aaaacagaag aagttttagt ggcttctacc
    38461 ggggttattg gggtacctat gccaatggat attatttgtc ctggtattaa acaaacgatt
    38521 gaaggtttag gtgattcaag agaagctgcc cttatggctg cagaagccat tatgacaacc
    38581 gatacttatc caaaagaaat agcggtttct tttacagtac aagagaagac cgttacaata
    38641 gctggaatgg ctaaaggttc aggaatgatt catcctaata tggccaccat gctgtcgttt
    38701 attacaacgg atttaaatat ttcctatgaa ttattggata aagcactaaa agaaagtatt
    38761 gcagattctt ataatatgat ttctgtagat ggagatacca gtactaatga catggttatt
    38821 gttcttgcta atggggctgc aggcaatgac cttattgaag aagaaaacga agattatgaa
    38881 gtctttaaaa aggccctaca ttatgtgaat tcccatttgg caaaagaaat cgtaagagat
    38941 ggcgaagggg ccggaaaatt tattgaggtt caagtaaaag ggactaaaac aaaagaagat
    39001 gccagaatac ttgccaagtc cattattaca tctaatttag taaagacagc cttttttggt
    39061 gaagacgcca attggggcag gattttatgt gccatgggct attcaggtgt ccagtttgat
    39121 gtgaatcatg taaccattga gttcaggtca aaagcaggaa aaattgcttt aatggaaaaa
    39181 ggacttccta tcttatttga tgaagaattg gccttaaaga ttttaaaaga aaaggatatc
    39241 caggttattg catgtcttca ggaaggagag gaagaagcca ctgcctgggg ctgcgattta
    39301 agttatgaat atgttcgtat aaatggggat tacaggacat agaaaggtga taaaatggag
    39361 aaactaatcg agaaagcaaa agttttagtt gaagcattgc catatattaa agagttttat
    39421 ggaaaaacgg ttgtgattaa atacggcggc agtgcgatga ttgatgaaag aataaaagaa
    39481 acggttattc aagatattgt tttaatgaaa ctggttggta tcaatccagt tattgttcac
    39541 gggggaggcc cggaaattaa taaagtatta gagcaaatga ataaaaaatc ccaatttatt
    39601 aatggcttaa gggtgactga taaagaaacc atggaagttg tcgagatggt tttatccgga
    39661 aaggtcaaca aagaaatcgt cagcgaaatt caaaatcaag ggattagttc tgtcggaata
    39721 agtggaaaag atggagggat gcttttagct gagaaaaaac tggtggacgg ctatgactta
    39781 ggttatgttg gacatgtggt cgccgttaat ccaagacttc ttttgaccct tattcaaacg
    39841 gactttatac cggttatagc acctattggt attgataaaa agggaaattc atataatatc
    39901 aatgctgatt atgctgcagt tgctgtagct ggagccttaa aggctgaaaa attagttttt
    39961 ttaaccgatg ttgaaggggt acttatggat gtagacgata gttcttctgt gatttcccag
    40021 ctttctataa aagatgtgga tcgttatatt gaaaaaggta tcatttcggg aggaatgatt
    40081 cccaaagtac agtgttgtgt tgaaggggta aaaatggggg taaaaactgt ccatatttta
    40141 gacggtcgga ttgagcattg tttactgctt gaaattttca cccataaagg tgttggaaca
    40201 atgattatgc aataaaggag aatcaaatgg ataaaagaga aaagaaaata agcagggctt
    40261 cctgggttgg tattataggc aatggtattc tggcagtttt aaaagtgttt ttttggtctt
    40321 atttctggta gcatggcatt agtaggagac ggtatcgatt cttttactga tatcgtaaca
    40381 ggttttatta ttttatttgc tgcaaaaatt atgtccaagc cgccagacag ggaacatcct
    40441 tatggtcatc agagggcaga gaccattgcc actgttttat tggcatttat catttttttt
    40501 gccggagctc aactgctgat taacacagta ccggccattt ttagtaaaga agaaacggat
    40561 ttaccttctg ccattgctat ttatattgct cttatatcca tactgggaaa actcttttta
    40621 gccttttatc aatacaaaat tggtaagctt actcaaagtt ccatgttgat tgccaatagt
    40681 aaaaatatga aaaatgatat tttaatttcc gtaggtgttt tgtttggttt gctttttacc
    40741 tttttgctta agctgcctgt ttttgatttt ataacagcca ttattattgc cctgtggatt
    40801 attaaagaag cttatgaaat tgtcatagaa agcaatatgg aactgatgga tggtgttgaa
    40861 gatacaagca tctatcagga agtctttgat gcaattgatc agatagaagg ggtctataat
    40921 ccccaccgaa ccagaatcag aaaaatgtct aatctctata tcattgatac agatattgaa
    40981 gtggatcaaa atttaacagt attagaagcc cataaaatag ctatgaaagt agaaaaaatc
    41041 ataaaagaaa aaattcaaga tgtctatgac gttattgtgc atgtggagcc tttaggcaat
    41101 attgagcata atgaaagtta tggcttatct cagaaaatgc ttcgtgaaaa aaaggactcc
    41161 caataggagt ccaagcccta aaaagcttca ttcctggaaa atacctatga cttagtctag
    41221 gtattttttt attgatttat tctttattga aagaataaat ttctgaagtt tacaaacacg
    41281 aataaggatg ggatggaata taataccact agaatgatat gaccaggagt gatatttatg
    41341 tggagatttc ccagtacgat atatgttgta aaagctggag attctctcta tcagatagcc
    41401 cgccagtttg gagtcagtgt gcaggacatt atcaatatca atggaattgt tgacacaatt
    41461 atttatcccg gacaaagatt aattatttcc atagcttatt atctggtcca gccaggagac
    41521 agcctttaca ggctggctca aaaatttaat acaacggttg aaagtattat gcgcctaaat
    41581 aatttgacca atattaactt aaggatagga caaaggcttc aagttcctca atattctgaa
    41641 gcaattgtaa ggacggcttt ggcgaatatt cgcaggaggc caggagtaaa ttttccaatt
    41701 gaaacggtta tggtaagagg ggcaaagttg cctgtaacgg ccagcagtaa tggctggtat
    41761 gaagtcgctt tatataatgg caggcgtggt tttattgcta aaagtcttgt tgattttaat
    41821 gtgtatggtt ccagtttacc tcttacaaat attttaggtt tttatactct ggaagaaggg
    41881 ccagccctac caagttctta tgaatctttt ataaataata caggagaaat atcccaactg
    41941 gctttattca tgtttagaat tgatgccgac aatcctacag aaatagaaaa attcggcgga
    42001 gattttagta atgcttacgt ggaggattta atcagaattg ctcacgaaaa taatattaaa
    42061 atttgtcctg tggtacacaa tttgctttat gaaagaggcg atgtgacagt tggtaaaaat
    42121 acagtaaaag caatggttgc aacgccggag tcccgggcgg cttttattca aaatattatt
    42181 gaacttattg agggttatgg ttttgatggg gttaatattg atatagaaga tgtttattta
    42241 gaagacagtg atcgtttaac ggccttttac agggaacttg gccgtgcatt gcaccaaaga
    42301 ggttatttct tttctgtttc tgttccttca
//
LOCUS       DAOSUD010000009        39455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_714_length_39455_cov_22.361371, whole genome shotgun sequence.
ACCESSION   DAOSUD010000009 DAOSUD010000000
VERSION     DAOSUD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 39455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39455
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_39455_cov_22.361371"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..800
                     /locus_tag="PLG49_02380"
     CDS             132..800
                     /locus_tag="PLG49_02380"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOQ16192.1"
                     /translation="MEIALNFFVPPGQHKLPPLPYDYDALEPIISSESLKIHHDILHR
                     AYVDNLNKTELKLVELREKDDFDLISYWENQLAFNGSGHILHSIYWTNLAAPGTGGRP
                     GSQTLNQINRYFGSFFSFQKQLSHAAEKVQGSGWAIVIWQPAWNRLEILQAEKHQNLT
                     QWGGIPILVFDVWEHAYYLDYQNRRQEYINRLWQLINWNDVERRLLLAMRGLVPIAME
                     NYRY"
     gene            complement(801..923)
                     /locus_tag="PLG49_02385"
     CDS             complement(801..923)
                     /locus_tag="PLG49_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16193.1"
                     /translation="MAIVPVPVGLLFLLGLGGVVIIAALIAIVVAFIANRNNDN"
     gene            complement(1176..1898)
                     /locus_tag="PLG49_02390"
     CDS             complement(1176..1898)
                     /locus_tag="PLG49_02390"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOQ16194.1"
                     /translation="MPYCPRCGVELDYHIKNCPLCQFPIPEIPEENNHINVEKFPRPE
                     NKYFEEILKVKNQIFFTLSILILSAVLVLMTINSIIETHPLAINYSIISVIAAWFYIL
                     ILLGYLSNLYYSILGIGIVTGCLTLALDSVDGKMNWFFTYALPIIILVLLIIYLFLYL
                     YNRSKLLNQFIFIPSYLFIAVALLSVGLECILDFQANQSIHLSWSLIVFIILVSLAVL
                     LLGLYYKLPEMIKEKIKRKLHI"
     gene            complement(1906..3153)
                     /locus_tag="PLG49_02395"
     CDS             complement(1906..3153)
                     /locus_tag="PLG49_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16195.1"
                     /translation="MKQKRAWYKLDNAGKLYPSITSTRVSTVFRISASLYNPVDPEIL
                     QKALNNIIGRFPYFKVNLKRGFFWYYFEYTEKYPQVQKETYYPCMFMLYKNKNSFPFR
                     VLYFNNRISVEFSHSITDGMGGIHFLKSLLIEYFRLLGIQPEFQEKISKEEAEDAFKR
                     YYVKNIPKPAKLKKAFHFPFKLNAKGEYYIITGIIPVNMLLEEAKKYSASLTQFLLAL
                     YFDTIQEFIRLHPCKLLPVVINLPVNLRNIFPSKTMRNFFISLTPQIDFRLGSYSFEE
                     IINYIRNYMAIALNKKHISQHIHLNVKREKTIFIRLIPLFLKNLMMPALYNKFAERNY
                     TTGLSNLGLITLPKSIENLVERFEVYPPPSMGNIIKVTTISYKDKIYISFGSLTKNTE
                     IEKIFFRKIRKMNIPVKIETNRE"
     gene            complement(3356..3778)
                     /locus_tag="PLG49_02400"
     CDS             complement(3356..3778)
                     /locus_tag="PLG49_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16196.1"
                     /translation="MMDYYDDTKKRQRPGPRPRPRPPYYNNNEPGYPQMPPFFSPPQV
                     PGRPPYTQAPSSSSGPPSNPPPAFLPQQSPSLYMIDPGAIRPCTFQFVYLWLEDGRSF
                     WAWLTFVGRRSVAGWRWFGNRWIYFGTDIDNISSFVCY"
     gene            4122..5108
                     /locus_tag="PLG49_02405"
     CDS             4122..5108
                     /locus_tag="PLG49_02405"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOQ16197.1"
                     /translation="MSKIRIGIIGYGNLGKGVELALKKNEDMDLIAVFTRRDPKSVVL
                     QSSQVPVVHIDAIDDYKDKIDVMILCGGSATDLPEQSPKFAKLFNIVDSFDTHSKVEE
                     HFAAVDKAAREGNKVAAISIGWDPGLFSLNRLLGDSILPDGVSYTFWGKGVSQGHSDA
                     IRRIKGVKNAIQYTIPIEEAIEEVRSGSNPELSAGQKHIRECYVVLEENADQTEVEKA
                     IKTMPNYFADYNTIVHFVSEEELKKNHSAMPHGGFVIRSGQTGNGGNKQIIEFSIKLD
                     SNPEFTASVLVAYARAVYRLNQEGQKGAKTIFDIPVAYTSAKSSEELRRAFL"
     gene            complement(5161..5595)
                     /locus_tag="PLG49_02410"
     CDS             complement(5161..5595)
                     /locus_tag="PLG49_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16198.1"
                     /translation="MPDGLTGTNFAALLARYIGETVTIFTSSGGQSGSGFTGVILSVN
                     NSFVRLLTRIGPPPGCSLGNACYDFNVAPWGYGGSPKKYGYEYENEYNAYSGEYGEGP
                     ILPADQNGAITAGGWNGYPVYTVGSVADIPIDSIVAFVHNAV"
     gene            5719..6609
                     /gene="queG"
                     /locus_tag="PLG49_02415"
     CDS             5719..6609
                     /gene="queG"
                     /locus_tag="PLG49_02415"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOQ16199.1"
                     /translation="MKKLLIAFANNLGIEYVGIAPAGPYDDLKEILEKRMVRGLKCEF
                     EEDEIKKRIDPSLTLENAKSVIVCLFPYYTGKIEMANLSMYTYGLDYHKIIMEKLEQI
                     GNFLTSRITDFRYKAFVDSGPLVDRYLAYKAGLGFYGINSHLINEKYGSYVFIGYMIN
                     NYPFEPDLAIEKTCIQCKKCIQACPGTAINGDFTINPFRCRSYISQKKGDLSQEEEEI
                     LRKDKLLFGCDICQDVCPHNERIDKTNLDEFQKNLIPFIDCDELLMISNKEFLRRYKN
                     RAFSWRGKNILIRNDKIIKK"
     gene            complement(6623..8914)
                     /locus_tag="PLG49_02420"
     CDS             complement(6623..8914)
                     /locus_tag="PLG49_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOQ16200.1"
                     /translation="MMKCSVCKKNIAIIFIPKMVRGKEEITGLCISCARKQGIGPVNQ
                     FLQQTGLSEEELDNLNEQMNEMFEDTDISELDEDNQNPFLKFLNNTFAGFRSSKDKDF
                     QPTDESNSEKTGSTTMDNHNNKKRKKRKYLDTYGINLTDKARNNEIDRIIGRQKEIDR
                     IIQILNRRTKNNPILIGEPGVGKTAIAEGLAVRIIEKQVPLKLFNTEIYLLDLTSVVA
                     GTQFRGQFESRMRGIIKEAKESGNIILVIDEIQNIIGAGNAEGGAMNAANILKPALAR
                     GEIQVIGATTLEEYRKHIEKDSALERRFQPVLVEEPTIEESIEIIKGIKDYYEEYHKV
                     SISDEVIEAAVKLSKRYIPDRFLPDKAIDIIDEASSRANLLNEGLVELKILKDELQMI
                     QNKKEEAAAQDNYEKAAEYKVIECRLQAQIAELEKQNKEVAITTDDVAHVIEAWTKIP
                     VQKITHLEAEKLLDLENRLHKRIVGQHKAVESISRAIRRNRSGFKKVQKPSSFIFVGP
                     TGVGKTELVKALTEELFGTEEALIRLDMSEYMEKHTVSKLIGSPPGYIGYDEGGQLTE
                     KVRRQPYSVILLDEIEKAHPDVFNMLLQILEDGRLTDSQGRTVHFENTILIMTSNAGT
                     SLKAHGIGFGKDNYEALENKVQEALKNTFRPEFLNRIDEVIVFTELSKEELRKIVDLM
                     LLEVKKDAKDRNISIHVDDEVKDYILEKGYDKKFGARPLRRTIQRLIEDELAERFLKG
                     EILEGTSVHIKYEDGQIQFTCQK"
     gene            9212..10975
                     /locus_tag="PLG49_02425"
     CDS             9212..10975
                     /locus_tag="PLG49_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16201.1"
                     /translation="MPIRLNELNPTNENEKKLIQYLAKSDDKRIEESLLLIENYTGYK
                     IKDFFGDSAFADIGLMLFIALLEKLSGQNIFKIYNKAFQAIIQKQYDNQLVRNKIIDD
                     ISAKLKISVDFLNELLLQFNDYVTQYATFCSKEEKDTDELLLYNNYNSIFEVFESPSR
                     HLSNSYANHPDDKPSTGMSDSHPGNVYSHRSDNTYPQYGNSPNDSPEDNFYDRELYQP
                     YRGVTTKQRKVYIPVALLTVIIVILFSTVFLSPDLLNIPAFIQDSHELLEVKGLTLKY
                     VLVAVSCEMYDVNVRIRGDQSEFYKEYGKDSYHHYLWIKDTYRQLPKDMKRHLETIYC
                     DGIYEGELIYLLADLEDEAGVNQIVYAIKTSKDIPVEVKAAAEEFYPYFYEEYLREYI
                     RMNRVMYDRHAEEMNKKIQEEKPNILEFIEETSGMQFERKYKPVIYYTMRPGNALGFI
                     YGDQFISLIPGTEHHYESLFFIPYHEFSHGIFNQITSTKEFKQVADTLKSNSKMRRTW
                     ENGFYDVYYSWYDWCEENLVEGFAMYLEYKAKGKLPDSIESIRPYDKAFFQYLVDIDF
                     NPETMNLKDVSIDFLESIADE"
     gene            complement(10976..11596)
                     /locus_tag="PLG49_02430"
     CDS             complement(10976..11596)
                     /locus_tag="PLG49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOQ16202.1"
                     /translation="MERIHFKRLPEHIGIIPDGNRRWALAHNKEKHEGYKYGIKPGFE
                     LYEICLEYGIKEMTFYGFTQDNTKRPAVQTRAFQEACVEAVMKLADRDANLMVIGNTD
                     SPLFPKELLPFANKRVLFGKGLININFLVNYGWKWDLMNNASKAIPRIDLIIRWGGRR
                     RLSGFLPIQSVYADFYVIDDLWPNFKRNHLDEALLWYQNQDITLGG"
     gene            11792..15115
                     /locus_tag="PLG49_02435"
     CDS             11792..15115
                     /locus_tag="PLG49_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOQ16203.1"
                     /translation="MKSLGLCVGASSIGEILIEKDKNNIRVIKQASVSHEGNPRKIIQ
                     EIMNAYKDDLPEKITITGRKFRKMIRASSISEVEAIEYAFEFIRQRDKRLEEVNLIIS
                     GGGESFLVYEIDKEGKIFNLYTGNKCASGTGEFFLQQLKRMNMTLEEARDCASLNEPY
                     KMAGRCSVFCKSDCTHALNKNIDKGRVIAGLCEMMADKIVELVRKSSFKKILFIGGCS
                     HNEGMLYYLKKKLGEVIIPSYADSFEALGAALWGLSHPTQTIDMSEDWLNQHKSSFSF
                     LEPLYLFKDKMTFKEAPKKEASAYDQCILGLDVGSTTTKAVLMRKKDNAIVASYYLRT
                     MGDPIRASKNCYKAIKEQLTSPVEIIGLGVTGSGRQIAALHALSPGIVNEIIAHARAA
                     LYFDSEVDTIFEIGGQDAKYTYIIKGVPADYTMNEACSAGTGSFLEEAAKESLFIDTK
                     EIEKIALESENPPNFNDQCAAFISSDIKNAIQEGIELKDIVAGLVYSICMNYINKVKG
                     NREIGKKIFMQGGVCYNKAVPIAMAALTGKEIIVPPEPGLMGAFGAALYIKEKLQLNL
                     IEPQKFDLEELFLREVNYRKPFVCAGGKEKCDRKCIIQRIEINNQIYPFGGACNKYYN
                     LTEQQEEKNTQTNYVLLREKMIFEEYSRMRAVRLAPKKNKTIGINKSLLTNTFFPLYY
                     QFFYGLGYDILLSDDKEKEGMERKGASFCYPVEISHGALENLIKKDPDIYFLPHIKSI
                     EVKNGFPVSVTCPLVQGEPYYLKASFDELAKKPVLSPVLDFAKGYDDLMDSFIKMAKD
                     LNEDVEIAKKSFQIAVNNQMDFYYKCKEIGKRILLDLEDEKDKLAFVLFGRPYNAFSS
                     WGNMGIPNKFATRNYVMIPFDFLPSDGEEAGDNMYWAMGQLILKAARMVKKHSQLFGV
                     YITNFSCGPDSFLNGYFRSIMDKKPSLVLELDNHTADAGIDTRIEAFIDIVKSYKQLS
                     DHKSSLEQDVFYPAEIEMKKGFAQVYDSSGQSYSLNDQRVHVLIPSMGDMASKLLVAT
                     FNYIGIQASALEEPGEEELKIGRGLSSCKECLPLQLTIGSLMKYLNERKNQDELLVYF
                     MPDTSGPCRFGQYSVLIKN"
     gene            15187..16101
                     /locus_tag="PLG49_02440"
     CDS             15187..16101
                     /locus_tag="PLG49_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16204.1"
                     /translation="MDFLLRGWHSVIISDVLEDIRSAILVLAKDKENGMKIYEEVSQE
                     ILLSARKDSWKKLKETLEASALKLKSIEIKMPVEKAVKVALVGEIYVRRDRFSRKKLV
                     EKLAQKNIMVKVAPVEEWMYYLDYIQKHRFTLNSTFRDRISTSIKGFFKNYYEKTIKQ
                     IFAKSGLYEYKVIEVNKIISNVKDLISPALTGEAILTIGSAITEIVDEVSGVISIGPF
                     GCMPGRIAEAVISEKMNTKKLLISPNKQLIEKVMKKHPALPFLSIETDGNVFPQIIEA
                     KLETFCLQVERLHQSIEKIKNNIEEISS"
     gene            16240..18063
                     /gene="typA"
                     /locus_tag="PLG49_02445"
     CDS             16240..18063
                     /gene="typA"
                     /locus_tag="PLG49_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOQ16205.1"
                     /translation="MIQKREDIRNIAIIAHVDHGKTSLVDELLKQSGIFRQNQEIAER
                     VLDSNDLERERGITILSKNTAVFYKNVKINIIDTPGHADFGGEVERVLKMVNGVILVV
                     DAFEGPMPQTKFVLKKALELELPVIVCINKIDRPEARPEEVIDEILDLFIELDADESQ
                     LDCPFVFASAKNGTASLDINSPGTDMQPLFETIIKHIPAPAGSEDEPLQLLISTIDYN
                     DYVGRIGIGKVERGSIKVNQEAVIVNAIDENKNKRVKITKIYQFEGLKRIPVENAKIG
                     DIIAISGIEDINIGDTVCDVNFPEPLPFVKISEPTIAMTFLVNDSPFAGQEGKFVTSR
                     NLRERLFKELQTDVSLRVEETSSTDAFRVSGRGELHLSILIETMRREGYEFQVSRPEV
                     LYKEIDGKLHEPMEMVTVDVPEEFVGTVIEKLGSRKGEMISMSSVKGAYTRLVFSIPS
                     RGLIGYRSEFLTDTKGNGIINAVFDGYAPYKGDIEKRQWGSLIAFETGEAVTYGLYHA
                     QERGMLFIEPGTKVYEGMIVGQNPKGEDIEVNVCKKKHVTNMRAAGSDEALRLSPPKK
                     MSLEECLEFIEDDELLEVTPKSLRIRKKILNSGERKKARKK"
     gene            18087..19700
                     /locus_tag="PLG49_02450"
     CDS             18087..19700
                     /locus_tag="PLG49_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOQ16206.1"
                     /translation="MKKKNSGGKLVKQAAVLAIASLIVRLMGLIYRWPLTNMIGDDGN
                     GLYGIAFNIYLFFFVISSGGLPAAISKMVSERMALKEYKSAHKVFKTALMLAASTGLT
                     CCLILWFGAQPIAKLLNNTRIVYSMKALAPTLLIVAIMGAFRGYYQGMNTMVPTAISQ
                     IIEQLFNAVFSIVLAAAFLKKGMEYAAAGGTMGTGIGALAGLIFLILLYILLRPRLLK
                     RVNQQTDSHFDESYQDILKTLLSISIPIIIGTAIFSITNLIDAKMVMSILQSLGMSEM
                     EANELYGQLTGKYVTLTNLPVSISSALAAAVVPSIAASMALKERKVVMNKVNLALRVS
                     MILAAPSMVGLFVLGDPILKMLFPNYPGGGDLLRIGSIAIIFLSVVQVSTAILQGVGK
                     VMVPARNAAIGTACKIIINYLLISVPLLNIKGAVISTVICYIIISFLDMKAVRKVTKV
                     KIDILGTYVKPIAASVVMGIFSRIFYKLFLWGSQSNTFATMGSIILSIVVYCSFLIVI
                     GGITKEDIFLLPKGELIYSKLTKMGMMQE"
     gene            19840..20085
                     /locus_tag="PLG49_02455"
     CDS             19840..20085
                     /locus_tag="PLG49_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16207.1"
                     /translation="MIMQRKIWILSAFVFLISSVFAYALYMHGYNKENRNLDHGKKFL
                     SILVRKKALRRPFRTGLSMRKSSLLYKESRTKSIHQL"
     gene            20121..20462
                     /locus_tag="PLG49_02460"
     CDS             20121..20462
                     /locus_tag="PLG49_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BofC C-terminal domain-containing protein"
                     /protein_id="HOQ16208.1"
                     /translation="MIEERVKAPYFLMDLTRDELQELYSDWQVSSFSSEKVVLRKKID
                     SKYSNQYVLGVYNGFVTIFSNNEEGYPEIKEITETPISSLPLEEQNKLEKGIEIYGEE
                     ELIRMLEDYTS"
     gene            20603..21862
                     /locus_tag="PLG49_02465"
     CDS             20603..21862
                     /locus_tag="PLG49_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ16209.1"
                     /translation="MSKKSKVLIIGGGAAGLMASIAAQLNGAQAVILERMNRVGKKIL
                     ATGNGRCNLTNVCLDIKNYHSSNIKFVHGILSRFSVEQTLDFFNQMGIEYKVEDKGKV
                     YPLSEQASSVLDLLRYEIQRLGIQEICEAEVVKIKKDRKKNNNAFTLFLKDGRTITGD
                     RVILATGGKSSPNLGSNGSGYQLVIPFGHKLIEPFPALVQLNLEAWFLKRLKGVKFNG
                     KAAILDKDKFLRTEEGEILFTDYGISGPPILQLSRKYGELLHKKNTRPLISLDLYPDF
                     SKEELKTKILSRFYYHPEKTIEFAFVGMINKRLIPVVLKEAGLNDLNIICKDIKDKEV
                     DNLINLFKDWRIPVKGTQSWMHSQVTAGGISLEDIHPATMESKLLPGLYFAGEVVDVD
                     GDCGGFNLQWAWSSGYIAGESAARTIG"
     gene            21947..22537
                     /locus_tag="PLG49_02470"
     CDS             21947..22537
                     /locus_tag="PLG49_02470"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOQ16210.1"
                     /translation="MDAIFSINSFQYSYIVLPVIIFLCRVVDVTMGTIRVIFVSKGYR
                     VGAALLGFFEVFIWITVIGEIMTNANSIFCYLAYAAGFATGNYVGIICENKLSIGLVV
                     VRIITQKNSDELIEYLRNENYGVTVLEATGSKGKVKIIFTVIKRKDLKAVIENINNFN
                     PKAFYSVEDVRTVNEGVFPKRDFDLMKAKALTRKEK"
     gene            complement(22729..23532)
                     /locus_tag="PLG49_02475"
     CDS             complement(22729..23532)
                     /locus_tag="PLG49_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOQ16211.1"
                     /translation="MFFADYHVHTSFSSDCQESMENQIQKAIELGLKEIALTDHVDFD
                     YPDPQFPFMIDYDKYMIEFDRIKEKYKDKINIILGVEIGYQPHIVNELNDFFSKYDFQ
                     FIICSMHACDRLDLYNGDFFKGKEQKNAYLRYFECVLDNVKKYNYYDVYGHLDYINRY
                     GNYENKILSYEDYSDIIDTILKTIIEAGKGIEINTSGFRYGLGHPSPQFAIVKRYKEL
                     GGEIITVGSDAHRKEDICSHFDCAYEMLKKAGFSKITVFKNRKASFVNI"
     gene            23706..24089
                     /locus_tag="PLG49_02480"
     CDS             23706..24089
                     /locus_tag="PLG49_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOQ16212.1"
                     /translation="MSEQNFKNHQHDDQCECGHEEHHDEDVIYLTLEDDTELKCHVLG
                     FFELDNKDYIALLPEGEYEVLIFNYVEDEEGIEINNIDDDEEFERVSDYFMNEFKDEL
                     EMMADDYDFDDDFDEDYDFDEEDEQ"
     gene            complement(24117..24737)
                     /gene="safA"
                     /locus_tag="PLG49_02485"
     CDS             complement(24117..24737)
                     /gene="safA"
                     /locus_tag="PLG49_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SafA/ExsA family spore coat assembly protein"
                     /protein_id="HOQ16213.1"
                     /translation="MKRKILLSLFVFIIFSVPTFAQSQTKTYIVQPGDSMWKIAVKHQ
                     IGLSEIIAANPQIKNPALIYPNQKLNIPNIDDVKSLENEVIKLVNAERAKNGLSPLKA
                     NWELSRVARMKSQDMINKNYFSHQSPTYGSPFQMMESFGIRYSSAGENIAKGQQSPSA
                     VMNAWMNSPGHRSNILSTSYSEIGVGLAVSKSGVKYWTQMFIRPLK"
     gene            24968..25813
                     /locus_tag="PLG49_02490"
     CDS             24968..25813
                     /locus_tag="PLG49_02490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOQ16214.1"
                     /translation="MREQVLFMKRTIENKEELIDVFSKNKIELITSHSTEETLDILDE
                     KFDKIDLLIIDMEEDPIFGLKSIKTIKKINDYKYIPIVIIANKKDVMKGLTMGAKEYF
                     IPPYDYEKLCSFVQIILKNKRENMHLYTPETSIDMSFEQYFNSEIKRAERGGYELAIL
                     ILTIVSKDHNLYTQERTILTMINQLVDLVRQNLRATDTIVRYNRSNVIAFLPYTSRFN
                     GEIVYEKILEIFNEKILANVDSKNNFEMVYSIASYPQDGDTVEDLLFNAERFLEDNKT
                     FLTGK"
     gene            25936..26798
                     /locus_tag="PLG49_02495"
                     /pseudo
     CDS             25936..26798
                     /locus_tag="PLG49_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
     gene            26860..27693
                     /gene="proB"
                     /locus_tag="PLG49_02500"
     CDS             26860..27693
                     /gene="proB"
                     /locus_tag="PLG49_02500"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOQ16215.1"
                     /translation="MDARKKLREAKRIVIKIGTSSLTHENGRIYYGKMERLARILSDL
                     KNEGKEIVLVSSGAIGIGSERLGWTQKPKQTEMKQAAAAVGQGILMQTYEKFFNEYNQ
                     IVAQILLTKDVLEDSVKKTNAQNTFKQLLKMGVIPIVNENDTVATEEIEESVFGDNDT
                     LSAMVSVLIDADLLIILSDIDGLYTKNPKDYADGVLIDTVEEITKEIEELAGGKGSSF
                     GTGGMITKICAAKIANSNGVDMVIANGDDLKNIIRILNGERIGTLFKKNTDIIPYTSV
                     I"
     gene            complement(27790..28206)
                     /locus_tag="PLG49_02505"
     CDS             complement(27790..28206)
                     /locus_tag="PLG49_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16216.1"
                     /translation="MKLQQQLEGEKSKEESKSSSDEKPGEKNETKQEEITLPEEELPE
                     NYPLLTASDSELKIAQAAVQITKHLPYMSDLFKVKTPSEILTLKYLIRDIKIASKLGE
                     WDLVHKDLKEIEEIWPKLQADITEKKNLSLFSLTKS"
     gene            complement(28224..28427)
                     /locus_tag="PLG49_02510"
     CDS             complement(28224..28427)
                     /locus_tag="PLG49_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16217.1"
                     /translation="MLSVSKKDWNSSAQQVKSLHSTWNSFFSEAYKKGMSAEKAEAFN
                     KDLNQLTSLVIAQAMEESRKKLN"
     gene            28826..29344
                     /locus_tag="PLG49_02515"
     CDS             28826..29344
                     /locus_tag="PLG49_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16218.1"
                     /translation="MNYCRECGKILDENQKECPFCGTYLGDQTAKEETGLSSNPLNQT
                     NPGPSTDNSFNYSSSGPGSSQDYGYKTTTDYSYSSMGGTGSMNNNPYSPMVTEPLNNW
                     VKVILSFVSSWIPLVGIIAGIIFMVKEDQDRKSFGQALLTMSIVVFVLCCICCVILAS
                     SGALAEMNPIIK"
     gene            29359..30009
                     /locus_tag="PLG49_02520"
     CDS             29359..30009
                     /locus_tag="PLG49_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOQ16219.1"
                     /translation="MRFFLYFLGRSVCHQIPERSFFIHNIQFPLCARCTGIYTGMFMG
                     FLYILIRKRHKGNKPPSIKYVILMIIFLLPMIIDGLTSYLGFRSTNNNIRIITGLMFG
                     VIIPVFIVLISNFDVQKHNTLPIIKNYMDILGMLLTIILPFFLIHFEHVIIWWLIASV
                     SVLIIPFIYVKIFYIILKNLFSSLKKKQVYFVASLFYLIFMVSLSLMNKFFLAKFR"
     gene            30020..30589
                     /locus_tag="PLG49_02525"
     CDS             30020..30589
                     /locus_tag="PLG49_02525"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOQ16220.1"
                     /translation="MDEKKNLRTVFLKKRNELSFEEVEKKSDRIYRKFINSNDYFHCK
                     ELFTYVSMKKEVSTVKIIKKALEDGKTVAVPKVDAIKREMFFSMIYSLDELETGHFGV
                     LEPKKECVRPVEASQYTLILVPGVVFDLNKNRIGYGGGYYDSYLRKYKDKVMKTIGLA
                     YNFQLIDRIPAQKHDIPLDLIVTDKDWLY"
     gene            30663..31262
                     /gene="ruvA"
                     /locus_tag="PLG49_02530"
     CDS             30663..31262
                     /gene="ruvA"
                     /locus_tag="PLG49_02530"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOQ16221.1"
                     /translation="MINYLKGTLEYADEGSLIIEVNGVGYKLAVPLSAMEKLPPLGGE
                     LKIYTYLQVKEDELCLYGFLSKEEQSVFEKLITVSGIGPKGAINILSVLSPMDIYMAI
                     VSADIKLLSSVSGIGKKTAQRIVLELKDKMGNLPVMDLNELQTVSLENNGLVEDALSA
                     LMALGYKRAEALQAVRQVYTEGMELEAVIKASLKKLSTI"
     gene            31281..32282
                     /gene="ruvB"
                     /locus_tag="PLG49_02535"
     CDS             31281..32282
                     /gene="ruvB"
                     /locus_tag="PLG49_02535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOQ16222.1"
                     /translation="MKDRIISAELRAEDFEMEAGIRPKTFDDYIGQEKVKNNLKVFIK
                     AAMNRKETLDHVLLYGPPGLGKTTLANIIANEMKVNIKITSGPAIEKPGDMAAILNNL
                     NEGDLLFIDEIHRLNRSIEEILYPAMEDYVIDIVIGKGPSARSIRLELPKFTLVGATT
                     RAGLLTAPLRDRFGVIQRLEFYTPSELAEIVSRSAKVLGIEIDSSGAYEIAKRSRGTP
                     RIANRLLKRVRDFAEVKYNSFIDEKVASLALEALEVDKMGLDRIDRKMIMTMIEKFGG
                     GPVGIDTLAAAIGEENDTIEDVYEPYLIQLGFINRTPRGRVVTDFAYKHFGIEQLKL"
     gene            32449..33039
                     /locus_tag="PLG49_02540"
     CDS             32449..33039
                     /locus_tag="PLG49_02540"
                     /inference="COORDINATES: protein motif:HMM:NF020450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HOQ16223.1"
                     /translation="MGGKKALNEERLMELAKKVSENEQLINFETFNEEMTISQVVRFF
                     KRNGKNFTKTMIQNYVRVGVIPPPVDKRYYTKNHLILLTLVDNLKLIYSLDEIKTLFG
                     PILKNTTSFDDDMIKVSDLYSDYIHLHAKALKDWKQYLPSTLEEVNKKLEDYHINENE
                     KDIASVFMLVLTIMAETIALKKLIDLISQEYLTNNE"
     gene            33114..33563
                     /gene="zapA"
                     /locus_tag="PLG49_02545"
     CDS             33114..33563
                     /gene="zapA"
                     /locus_tag="PLG49_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOQ16224.1"
                     /translation="MEKKNKIEVIINGKVYTLVGNESEEYIQRVALYIDKKMNEIKNA
                     EASKTLSTSMIAILTSINVADDLFKLSESMNHLEKRMKDLEKVLEDKNKQIKSYQSEL
                     ANLRKENRELRDTISKQEVEIKKSQMELEEYINIFDSNVNNRISGDK"
     gene            33654..34670
                     /locus_tag="PLG49_02550"
     CDS             33654..34670
                     /locus_tag="PLG49_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOQ16225.1"
                     /translation="MDKRSVELLAPVGTKESVKAAVSNGCDAIYLGGKAFSARQYADN
                     FTIEELKEIIDYCHLRNVKVYITVNTLYKQKEMDDLFDFLNQLYQLGVDAFIVQDIGT
                     AVEIKKVFPKISLHASTQMTIHNLEGVKFLESLRFDRVVLSRELSLEEISYITERTNL
                     EIEVFVHGALCFSYSGQCLMSSMIGGRSGNRGRCAQPCRLPYRLMGENHREMMSGYLL
                     SPKDIQTLDYIPQMIEAGIKSFKIEGRMKRPEYVGAVAGIYRKYIDLYFEKADDYLVD
                     EQDIKILAQLFNRGGFSKGYLFSQGSKEMMSFENPKHWGIYAGKVKDYDVRTKNALSI
                     QLWI"
     gene            34661..36046
                     /locus_tag="PLG49_02555"
     CDS             34661..36046
                     /locus_tag="PLG49_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOQ16226.1"
                     /translation="MDLKAGDGIEIWRKEKENTGVEISKASKAGEKISIYLNEPVKKG
                     DLVYKTKDKELLEQIQKTYSKDIRKYNIYGNLRFKKHMPLLLQLWDDQGNFFELAGPV
                     VEEAKNQPLTEERIKAQISKTGNTPFIFKSINIDCDQDAYLAISELNLIRRKAIEAFA
                     NIIIEKSRRDTSLNKRNLMVNIKESIMNPNKVFNVLLKNLEDIEYALHPKVKRIYMDL
                     NHYTNADIEKAARLCHKHNKALFLALPRIDLNYKKEKIWYNLENSSIDGYLLRTYGQV
                     YQLKNSNKEKILDYTFNIFNQLSVDYWLNHGMKALTLSPELNYDELKVFYGKGLEIIG
                     YGYLPLMVTKQCVIGNTIGKRSSSCPCHCKGKKERYKLMDRKNEAFPIEQDCSSCLMM
                     IYNSKPLLLLKDLDKILSLPISSLRLDFYLEEKNEIKDIIESYIDKPEIYFKKYKDKE
                     YTKGHYFRGVE"
     gene            36057..37424
                     /locus_tag="PLG49_02560"
     CDS             36057..37424
                     /locus_tag="PLG49_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOQ16227.1"
                     /translation="MLKESIIDILILISRYAFVLFIFIFLWSGYKAIIFEKENETGEF
                     INKIYMQRFMIVLTHVLAFVILMMTSKDSVQIIEIMKLSAFGLAFIILFMIFTSLIYK
                     KSDPVIWNCILFLMVVGLIMLERIDHDLAKKQIIWFLAGTFVMIFIPLVFKIVPRFEA
                     FKYLYLILGWIFILSPFILGKERYGAYNWIYIGNFAFQPSEIVKFLFIFYLASALRAY
                     HSIKDLILPSSMAGGYVLVLVLQRDLGGALIFFLTFLILVYVRTSSSLLFFGGLGGAS
                     LASVLAYKLFSHVQVRVQAWLNPWKEIDTKGYQITQSLFAIGTWGWLGSGLTAGYPKH
                     IPVVESDFIFSAICEEFGNLFSIGIILVFLLIILRGTLIALKSNRIFYSLLAMGAVSL
                     IAIQTFLIIGGVIKMIPLTGVTLPFISYGGSSVIVSIMITGILQWIQSYYATREEVLE
                     YDDSL"
     gene            37411..38808
                     /locus_tag="PLG49_02565"
     CDS             37411..38808
                     /locus_tag="PLG49_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ16228.1"
                     /translation="MIPYKKSIKKVFWLYALMFCALIGYLLKFLMVDSSSVIVNTYNP
                     RLSALEKNIHRGEIRDRDGEVLARTVENGDRLIREYPKGRDFAHVVGYVQKGKTGVEA
                     YGNFILLEVSDRFIQRVNQVFTGDKLKGNNIILTLDADIQHKARELLKGRKGAIVVME
                     PSTGKILAMVSYPDFNPNEIDENWAKLNQDEENSPLLNRASQGLYPPGSVYKIVTAAA
                     TIENMENWQDFHYRCSGEANFKGNSIRCYNSQAHGEVDLEKAFTVSCNTAFSQLGVDL
                     GGEKMQLISERVLFNRDLPYELEYSKGRFALTRNSEEKEIIETAIGQGKTLVSPLHMA
                     MITSAIANGGILMKPYIIDHVETNYGREKNKQIPQKYDVLFSPEVASQLTEMMVRVVN
                     EGTGSQAKIKNVTVAGKTGTAQNASGEDHAWFVGFAPAEKPEVVVAVIIENGGSGGRI
                     AGPIAKELMQMVINQ"
     gene            38822..>39455
                     /locus_tag="PLG49_02570"
     CDS             38822..>39455
                     /locus_tag="PLG49_02570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOQ16229.1"
                     /translation="MLTLFQLALAPVIITCFYIYIRDKYEKEPLRLLLLGLFFGLLIS
                     TPIMAVEILLTNITPEGNHLRETLFTAFVVAAGTEEFFKYLVLYFLIWKNRNFNEPFD
                     GIVYAVFISLGFAGLENILYVLNPYLGGFQTAAARAVFSVPGHALFGVSMGYYYAIAK
                     FNQNKKSMLITAFFVPWLFHGIYDFILILNNSLLMILFVPFVLYLWIQGMR"
BASE COUNT        13293 a         5546 c         7643 g        12973 t
ORIGIN      
        1 tctaattaaa taataacatg atttttataa taatgcaata gaaaaatcta tttggggaat
       61 ttttaaggac tcatgattaa aagaggagac taatatacat attatgtatg atttttttgg
      121 gaggaataag aatggaaatt gctttgaatt tttttgttcc accaggacag cacaagttgc
      181 ccccactacc ttatgattat gacgcccttg aaccaattat tagttctgaa agccttaaga
      241 ttcatcatga tatactgcac agggcctatg tagataattt aaataaaaca gaactcaaac
      301 ttgtagaatt aagggaaaaa gatgattttg atttgatcag ttattgggaa aatcaattgg
      361 cttttaatgg ctcaggacat attttacaca gcatttactg gaccaatctg gctgctccgg
      421 gaactggggg aagaccagga agtcagacat taaatcaaat caaccgctat tttggcagtt
      481 tttttagttt tcaaaaacag cttagtcatg cggctgaaaa agttcaaggg tcaggttggg
      541 ctatagtgat ttggcaacct gcctggaaca gattagaaat tcttcaggct gagaaacacc
      601 agaatttaac ccaatggggt ggcattccca tattggtttt tgatgtttgg gaacatgcct
      661 attatttaga ttatcaaaac agacgacaag aatatatcaa ccgtttatgg cagttgatta
      721 actggaatga tgtagaaaga aggttgttat tggctatgag gggtttagta cccattgcta
      781 tggaaaatta cagatattaa ttaattgtca ttgttacggt tagctataaa tgcaacaacg
      841 atagcgataa gtgctgcaat aataacaacg ccgcctaatc ctaaaagaaa taatagtcct
      901 actggtacag gtactattgc catgtttttc cctcctaatg aatgacttat aaatatagaa
      961 taaaagataa taacttattt ttcaagatat agaatgaatt tttaagattg cttataaaat
     1021 gacaaaaaaa taaccactac ctgattggta gtagctatga atatatgtga attgaaggag
     1081 tatgttactg atttattttt attataatag gaaaatatga actagatgtg aacatatctt
     1141 tacaatttaa ataaaaataa ttggaaaagg taaactcaga tgtgcagttt tctttttatt
     1201 ttttctttaa tcatttccgg aagtttataa tataaaccca gcagcaatac agcaagggaa
     1261 accagtatta taaatacaat cagggaccag gataagtgga ttgactgatt agcctgaaag
     1321 tctaaaatac attccagccc tacagatagt aaagctacag caataaataa atatgaaggt
     1381 ataaatataa attggtttaa aagtttactg cggttataaa gatagagaaa aaggtatata
     1441 atcagcaaaa ctaaaattat gatgggtaaa gcatatgtaa aaaaccagtt cattttacca
     1501 tctacagagt caagagctaa ggttaaacaa cctgttacga ttccgatacc taatatggaa
     1561 taatagaggt tagaaagata tcccaacaag ataagaatat aaaaccaggc ggcaatgaca
     1621 ctgatgatac tataattaat ggccaaagga tgagtctcta tgatagaatt aatggtcatt
     1681 aataccagga cggcacttaa aatcagaatg gataaggtga aaaatatctg atttttaacc
     1741 ttaagaattt cttcaaaata tttattttct ggtcggggaa atttttctac atttatatgg
     1801 ttattttctt ctggtatttc tggaataggg aattgacata gagggcagtt tttaatatga
     1861 taatccagtt ctacgccgca tctgggacaa taaggcattt tctaatcact ccctgtttgt
     1921 ttctatttta actggaatat tcatttttct tatttttcga aagaaaattt tttctatttc
     1981 tgtatttttg gttaagcttc caaaagatat gtatattttg tctttataac taattgtggt
     2041 tactttgatg atattaccca tactgggagg gggatatact tcaaaacgtt cgaccaaatt
     2101 ttctatcgat tttggaagcg taattaatcc taaattggat aagcctgttg tataatttct
     2161 ttcagcaaat ttattgtata aagcaggcat cataagattt tttaaaaaaa ggggaattaa
     2221 acggataaaa attgtttttt ccctttttac attcagatgt atatgttggc tgatatgttt
     2281 tttatttagg gcaattgcca tataatttcg gatataattg atgatttctt caaagctata
     2341 gctgcccaga cgaaagtcaa tctgtggtgt aaggctaata aaaaaatttc tcatcgtttt
     2401 tgatggaaag atatttctta gattaacggg caaattaatg accacgggca gcaatttaca
     2461 aggatgtaag cggataaatt cctggattgt atcaaaatag agggccagta aaaactgggt
     2521 taaactggca ctatattttt tggcttcttc aagaagcata tttactggga taattcctgt
     2581 aataatatag tattctcctt ttgcatttaa tttaaatgga aagtgaaaag ctttttttaa
     2641 ttttgctggt ttagggatat ttttaacata atatctttta aaggcatctt ctgcttcttc
     2701 cttcgagatt ttttcctgaa actctggctg gattccaagc aaacggaaat attctataag
     2761 gagacttttt aaaaaatgaa taccacccat accatctgtt atactgtgag aaaattcaac
     2821 actaatccgg ttattgaaat acaataccct gaaaggaaaa gagtttttat ttttatataa
     2881 catgaacata caaggataat atgtttcttt ttgaacctgg ggatattttt cagtgtactc
     2941 aaaataatac caaaaaaaac ccctttttaa attaacttta aaataaggaa aacgacctat
     3001 gatgttattt aaagcttttt gcaggatttc aggatctaca ggattatata gagaggctga
     3061 aattctaaaa acagtagaga ctcttgtaga agtaatagaa ggatatagtt ttccggcatt
     3121 atcgagctta taccatgctc ttttttgttt cataataatc accaatttgt aattgaaaaa
     3181 tttttatcct taatgtttcc tattatatta tgtataaaaa tgaaagacaa gagttaaatt
     3241 tgtatattaa ttactgaaat ttaaggagca ggaatcaata ttttatgatg aataaaagaa
     3301 gctgtcttaa gattttcaga cagcttcttt tttgattcaa aaatttatgt tatgcttaat
     3361 aacatacaaa gctggatata ttatctatat ccgtaccaaa atatatccaa cggtttccaa
     3421 accatcgcca gcctgcaaca gagcgcctgc ctacaaaggt aagccatgcc cagaaacttc
     3481 ttccatcttc cagccataga taaacaaact ggaaggtgca gggacggatg gcaccaggat
     3541 cgatcatata taaacttgga ctttgctgtg gtaaaaaagc tggaggaggg tttgacggag
     3601 gtccggaaga actggatggt gcctgtgtgt aaggcggcct tccaggaacc tgaggcggac
     3661 taaaaaaagg aggcatttgt ggatagcctg gttcattatt attataataa ggaggtcttg
     3721 gccttggacg aggacctggt ctttgccgtt tctttgtgtc atcataataa tccatcatat
     3781 cactcctaat aattttttag tgtattatat gcagtaaaat atgaaaatgc gacagcaata
     3841 aagactaaaa tttttataaa aaactagaag cattaaaaaa gctttgttaa agaaaaatgt
     3901 ttaaagataa tgaataattc tatgtgaaat gaagacaata aaatcgcgtt caggcgatta
     3961 aaattgctta aaagattatc aatgttaaaa aaatgaagga tataattcaa aaacttgcag
     4021 ttttcttaat caaatgataa aatgataata tcatttgtat tattaaaagg tattttgata
     4081 atatagataa aattcctata aggttaattt aaggaggatt tatgagtaaa ataagaattg
     4141 gtataattgg ttatggaaat ttgggaaaag gtgtggaatt agcactaaaa aagaatgaag
     4201 atatggattt aattgcagtt tttacaagac gggatccaaa aagtgttgtg cttcaaagca
     4261 gtcaggtgcc tgttgtacat attgacgcaa ttgatgatta taaagacaaa attgatgtta
     4321 tgattttatg cggcggttca gcaacagatt tgcctgaaca aagtccaaaa tttgcaaaac
     4381 tttttaacat cgtggatagt tttgatacac acagtaaagt agaagagcat tttgcagccg
     4441 tggataaggc tgcaagagaa ggcaataaag ttgctgctat ttctatcgga tgggatccag
     4501 gtcttttctc tctaaaccgt ttattaggag actccatttt accagatgga gtaagttaca
     4561 ctttttgggg aaaaggtgta agtcagggac attccgatgc tataaggcgt attaaaggtg
     4621 ttaagaatgc cattcaatat actattccta tagaagaagc cattgaagag gtacgctcag
     4681 gttctaaccc tgaattatcg gcaggacaaa aacatatccg ggaatgctat gttgtactgg
     4741 aagaaaatgc agatcaaaca gaggttgaaa aggcgattaa aaccatgccc aattattttg
     4801 cagattacaa tactatcgta cactttgttt ctgaagaaga gctaaaaaag aaccattctg
     4861 caatgcctca tggtggcttt gtcataagaa gcggtcaaac gggaaatggt ggcaataagc
     4921 aaatcatcga gttttctatt aaattagaca gtaatcctga atttacagcc agtgtactgg
     4981 ttgcttatgc cagagctgta taccgcttga atcaggaagg acaaaagggc gcaaagacca
     5041 tatttgatat tcctgttgct tatacatcag ccaaatcttc tgaagaatta agaagagctt
     5101 ttctgtaaga aaaatatacc ccccttgttt acagggggtt tttgctaagt ttttatactt
     5161 ttatacggca ttatgaacaa aggctacaat gctgtcgatg gggatatctg ctacagaacc
     5221 tacggtgtat acgggatagc cattccagcc tcctgcggta atagcaccat tctgatccgc
     5281 aggaaggata ggtccttctc catattctcc cgaataagca ttgtattcat tttcatattc
     5341 atagccgtat tttttaggac ttccgccata tccccatggt gctacattaa agtcatagca
     5401 ggcattgcct aaggagcatc ctggtggagg tccaattctg gttagtaaac ggacaaagga
     5461 attattaacg gataaaataa cgcctgtaaa tcctgaccct gattgtccgc cgctggaagt
     5521 aaatattgta actgtttcac caatatatct tgccagtaag gctgcaaagt ttgttcctgt
     5581 aagaccgtcc ggcatatgat ttcctccttt ttaaaggata aattaaaata tctactttat
     5641 aatattcaaa tttaaaaaaa gtgtgttcca tagacaggat aaaaattatt aatcatatta
     5701 aaataatgga gatttactat gaagaaatta ttaattgctt ttgcaaacaa ccttggaatt
     5761 gaatatgttg gcattgcacc tgcaggacct tatgatgatc tgaaagaaat tcttgaaaaa
     5821 agaatggtca gaggattaaa gtgcgagttt gaagaggatg aaatcaaaaa aaggatagac
     5881 ccttccttaa ctttggaaaa tgcaaaatca gtcattgtct gtttatttcc ctactataca
     5941 ggcaaaatag aaatggccaa tttatcaatg tatacttatg gcttagatta tcataaaatt
     6001 attatggaaa aattagagca gataggcaac tttttaacaa gtaggattac tgattttcga
     6061 tacaaggcat ttgtggattc aggtcctctg gtggataggt atttggctta taaggctggt
     6121 ttgggttttt atggaatcaa ctcgcatctt attaatgaaa agtatggaag ttatgtcttt
     6181 attggctata tgattaataa ttatccgttt gagcctgatc tggctataga aaagacatgc
     6241 atacagtgca aaaaatgtat tcaggcatgt ccagggactg caattaatgg tgattttaca
     6301 attaatcctt ttcgttgcag gtcttatatc agtcagaaga agggcgattt atcgcaagag
     6361 gaagaagaaa tcttaagaaa ggacaaatta ctttttggct gtgatatttg tcaggatgta
     6421 tgtcctcata acgagaggat tgataaaaca aatttggacg agttccaaaa aaatttaata
     6481 ccttttattg attgtgatga gttattaatg atatccaata aagaattttt aagacggtac
     6541 aaaaataggg cttttagttg gagaggaaaa aatatactta ttagaaatga taaaattatt
     6601 aaaaaatagc acccgaaggg tgctattttt gacaggtaaa ctggatttgc ccatcttcat
     6661 atttgatatg aacactggtt ccttcaagaa tttctccttt aagaaagcgc tcagccagtt
     6721 catcttcgat gagcctttga atggttcttc tgagtggtct tgcaccaaat tttttatcgt
     6781 atcctttttc aaggatatag tctttgactt catcatcgac atgaatgctg atattcctat
     6841 cttttgcatc ttttttaact tctagtaaca ttaagtctac gatttttctt aattcttctt
     6901 ttgatagttc cgtaaacacg atgacttcat caattcggtt taagaattca ggacggaatg
     6961 tattttttaa tgcttcctga actttgtttt caagagcttc atagttatcc tttccaaaac
     7021 caataccatg agccttaagg ctcgttcctg catttgaagt cataatcagt atggtatttt
     7081 caaagtggac ggtacgaccc tggctgtctg taaggcgacc atcttccagt atttgaagaa
     7141 gcatattaaa aacatccgga tgagcttttt caatttcatc caggaggatg acagaatagg
     7201 gctgtcttct gactttttca gttagctgtc caccttcatc ataacctata tagccaggag
     7261 gagatccaat gagcttggat acagtatgtt tttccatata ttctgacata tctaagcgga
     7321 ttaaggcttc ttccgttcca aataattctt ctgtgagagc tttgaccagc tcagtttttc
     7381 cgactcctgt aggtcctaca aatataaagg aagaaggttt ttgtactttc ttaaaccctg
     7441 aacggtttct tcgtatggct cttgaaatac tttcaacagc tttgtgctgg cctacaattc
     7501 ttttatgaag tctgttttct aagtctaaaa gtttttctgc ttccaaatga gtgatttttt
     7561 gtacagggat tttagtccag gcttcaatga cgtgggcaac atcatctgtg gtaattgcaa
     7621 cttctttgtt ttgtttttct aattcagcta tttgggcttg cagtctgcat tctattactt
     7681 tatattctgc tgctttttca taattatcct gggctgcagc ttcttctttt ttattttgaa
     7741 tcatttgcaa ttcgtctttc agaattttta attcaactaa accttcattt aaaagattag
     7801 ctcttgaact tgcttcatca ataatatcaa tggctttatc cggcagaaat ctatcgggta
     7861 tatatctttt tgataatttc acagccgctt caatcacttc gtctgatatg gataccttat
     7921 ggtattcttc ataatagtct ttgattcctt ttataatttc tatgctttct tcgatggtag
     7981 gttcttcaac taagacaggt tgaaagcggc gttccagggc agaatctttt tcaatatgct
     8041 tcctgtattc ttctaatgtg gttgcaccaa tcacttgtat ttcaccacgg gccaaagcag
     8101 gttttaaaat attagcagca ttcattgcgc ctccttcggc attaccagcg ccaataatat
     8161 tttgtatttc gtcaatgact aaaataatat tgccagattc tttagcttct ttaataattc
     8221 ctcgcattct gctttcaaat tgtcccctga attgagttcc tgcaacaact gatgttaagt
     8281 ccaaaagata aatttctgta ttaaatagtt ttaatggaac ttgcttttcg ataattctta
     8341 ctgctaagcc ttcagcaatt gcggttttgc caacgcctgg ctctccaata agaataggat
     8401 tattttttgt acggcggttc aggatttgaa tgattcggtc aatttccttt tgtctgccaa
     8461 taattctatc aatttcgtta tttctggctt tgtctgtaag gttaatacca taggtatcca
     8521 ggtattttct ttttttgcgt tttttattat tatggttatc catggtagtt gatccggttt
     8581 tttctgaatt agattcatct gttggctgaa aatccttgtc ttttgagctt ctaaatcctg
     8641 caaaggtatt attaaggaat tttaaaaaag gattttgatt gtcttcatct aattcactga
     8701 tatctgtatc ttcaaacatc tcgttcattt gctcattaag attatctaat tcttcttcag
     8761 ataaaccagt ttgctgtaaa aattgattaa caggaccaat accttgtttt cttgcgcaag
     8821 agatacaaag gccagtgatt tcttccttgc cccttaccat tttaggaatg aagataatgg
     8881 ctatattttt tttgcaaaca gaacatttca tcatagaaac cactccatgt gtgtcaagat
     8941 tattcttagg atattataca taaaaattta ttatgtataa ataaaattat aattacattt
     9001 tttttataaa tagtataaca ttttttgaag aatacaggta tatgaaaata ataatccagt
     9061 caagtatatt aacatatttt atttcatagt taaatcagaa ataaaagatt ttttcaatct
     9121 ggatttttat ttggttatgg tttattatta tattatctta atatattgtc aatttacaga
     9181 aactataatt ttaaggtgga atgataaaaa aatgcctatt cgtttaaatg agcttaatcc
     9241 aacgaatgaa aatgaaaaga aattaataca gtatcttgct aaatcagatg acaaaagaat
     9301 agaggagagt cttttattaa tcgaaaatta tacgggatat aaaattaagg attttttcgg
     9361 tgattcagca tttgctgata tcggattaat gctctttatt gctttattag aaaagttgag
     9421 cgggcaaaat atatttaaaa tatataataa agcttttcag gctattatac aaaaacaata
     9481 tgataatcag cttgtaagaa ataaaattat agatgatata tctgcaaagc ttaaaatatc
     9541 tgtagatttt ttaaatgagc tcttattaca atttaatgat tatgttactc agtacgcaac
     9601 tttttgtagt aaagaagaaa aagatactga tgagcttctt ttatataata attataatag
     9661 catatttgaa gtttttgaaa gtccttcaag acacctgagc aacagctatg ccaatcatcc
     9721 tgatgacaaa ccttcaactg gtatgtctga cagccatccc ggcaatgtct atagtcatcg
     9781 ttcagataat acatatcctc aatatgggaa cagtcccaat gattcacctg aggataattt
     9841 ttatgacagg gaattatatc agccttatag aggtgtaaca accaaacaga gaaaagtcta
     9901 tattcctgtg gctttattga ctgtcatcat tgtgatattg ttttctacag tttttttatc
     9961 acctgatctg ttgaatattc ctgcatttat ccaggattct catgaacttt tagaagttaa
    10021 aggtttaact ttaaaatatg ttttagtagc tgtaagttgt gaaatgtatg atgtgaatgt
    10081 acggattcga ggtgatcaat cggaatttta taaggagtat ggaaaagatt cgtatcatca
    10141 ttatctttgg ataaaagata cctatcggca gcttcctaaa gacatgaaaa ggcatttaga
    10201 aacgatctat tgtgatggga tatatgaagg agaattaatc tatttgctgg cggacttaga
    10261 agatgaagca ggtgtcaatc aaattgttta tgcaatcaaa acgagcaaag atatacctgt
    10321 agaagttaaa gcagccgcag aagaatttta tccgtatttt tatgaagagt atttaagaga
    10381 atatatccga atgaataggg ttatgtatga taggcatgca gaagaaatga ataaaaaaat
    10441 tcaagaggaa aagcctaata ttctggagtt tattgaagaa acttcaggaa tgcaatttga
    10501 aagaaaatat aagccggtca tatattacac gatgcggcct gggaatgctt taggttttat
    10561 atatggagat caatttatat cgttaattcc aggtactgag caccattatg agagtctgtt
    10621 ttttattccc taccatgaat tttcccatgg gatattcaat caaataactt caacgaaaga
    10681 atttaagcag gttgcagata ctttaaaatc aaatagtaaa atgcgcagga catgggaaaa
    10741 tggtttttat gacgtttatt acagttggta tgactggtgt gaagaaaatc tggtagaagg
    10801 ttttgctatg tatttagaat acaaggcaaa aggaaagctt cctgattcca tagaatctat
    10861 aagaccttat gacaaggcat tttttcagta tttagttgat atagatttta atcctgaaac
    10921 gatgaatttg aaagatgtga gtattgactt tttagaaagc attgctgacg aataattatc
    10981 cccccagtgt aatatcttga ttttgatacc ataaaagggc ttcatccagg tgatttcttt
    11041 taaaatttgg ccataagtca tcaatcacat aaaaatctgc ataaaccgat tgtattggta
    11101 aaaaaccgct taatctgcgt cttcctcccc agcggataat caaatcaatc ctgggaatag
    11161 cttttgatgc attattcatt aaatcccact tccatccata atttactaaa aagtttatgt
    11221 tgatgagtcc tttaccaaaa aggactcttt tattggcaaa aggaaggagt tcttttggga
    11281 aaaggggaga atctgtattt ccgatgacca ttaagttagc atctctgtca gccaatttca
    11341 ttacggcttc tacgcaggct tcctgaaagg ccctggtttg aactgcaggc ctttttgtat
    11401 tgtcctgagt aaaaccataa aaagtcattt ctttaatgcc atattctaga cagatttcat
    11461 ataattcaaa tccaggtttg attccatatt tatagccttc gtgtttttct ttattgtgtg
    11521 ccagtgccca tctcctgttt ccatcaggta taatacctat atgctcaggg agtcttttaa
    11581 aatgaattct ttccataaaa gcccttcttt cttaataata acattttatc agaagagttt
    11641 attagtaaga ttccccaaat tcaatttttg tattcaaaat aaaaagctga taagcctgta
    11701 taacctgtat aaaaagtagc gatttaaatg tttgaagcct cgcaaaactg gataaaatct
    11761 caaataagaa ctgatgaatg gagggaagaa tttgaaatct ttaggtttat gcgttggggc
    11821 ttccagtatt ggtgaaattc ttatagaaaa agataagaat aatatcagag ttattaaaca
    11881 ggcttctgtt tcccatgaag gaaatccaag aaagatcatt caggaaatca tgaatgctta
    11941 taaagatgac ttgcctgaaa aaattacaat aactggccgg aaattcagga agatgattcg
    12001 ggcttcatcg atttctgaag ttgaagccat agaatatgca tttgaattta tacgtcaaag
    12061 ggataaaaga cttgaagagg taaatcttat tataagcggt gggggagaga gttttttagt
    12121 ttatgaaata gacaaagaag ggaaaatatt taatctgtat acaggaaata aatgcgcctc
    12181 cggaacggga gaattttttt tgcaacagtt aaaaagaatg aatatgacgc ttgaagaagc
    12241 cagggattgt gcaagtctta atgaacctta taagatggct gggagatgtt cggttttttg
    12301 taaaagtgat tgtacccatg ctcttaacaa aaatatagat aaaggtagag ttattgctgg
    12361 tctgtgcgaa atgatggcag ataagattgt tgaattagtc agaaagagtt cttttaagaa
    12421 aattttattc attggaggtt gttcacacaa tgaaggaatg ctttattacc ttaagaaaaa
    12481 gttaggagaa gttataattc cttcatatgc tgacagtttt gaagctttgg gtgctgcttt
    12541 atggggcctt agtcatccga ctcaaacaat tgatatgagt gaagattggt taaatcagca
    12601 taaaagttct ttttcatttc ttgagccttt atatctgttt aaggataaga tgacttttaa
    12661 ggaagctccc aaaaaagaag cttctgctta tgatcagtgt attttgggtc tggatgtggg
    12721 ttcaaccaca actaaagctg ttttaatgag aaaaaaggat aatgctattg tggcttctta
    12781 ctatctgaga accatgggtg accctataag agcctccaaa aactgttata aagccattaa
    12841 ggaacagctg acctctccag ttgagattat tggtttgggt gtaacggggt ctggcagaca
    12901 aattgctgcc cttcatgccc ttagtccggg tatcgtaaat gagattattg ctcatgccag
    12961 ggctgcttta tactttgatt cggaagtaga cacaattttt gaaattggtg gtcaggatgc
    13021 aaaatatact tacattatca aaggagtgcc tgcagattat acaatgaatg aagcctgttc
    13081 agccggtaca ggttctttcc tggaagaagc tgcaaaagaa tctttattta tcgatacaaa
    13141 ggagatagaa aagattgccc tggaaagtga aaatccgcct aattttaacg atcaatgcgc
    13201 tgcatttatc agcagtgata taaaaaatgc tatccaggaa ggtattgaat taaaggatat
    13261 agtagcaggc ctggtctatt ccatatgcat gaattatatt aataaagtta aagggaacag
    13321 ggagatcgga aaaaagattt ttatgcaagg cggagtatgc tacaacaagg cagtacccat
    13381 agccatggct gcccttacag gaaaagaaat tattgtccca ccggaaccag gattaatggg
    13441 agcttttggt gctgccttat atattaagga aaaactccag ttaaatttaa tagaaccaca
    13501 aaaatttgat ttagaagaat tattcctaag agaagtcaac tacagaaagc cgtttgtatg
    13561 tgccggaggg aaagaaaaat gtgacagaaa atgtatcatt caaagaattg aaatcaataa
    13621 tcagatatat ccctttggtg gcgcctgcaa taagtattat aatctgacag agcagcagga
    13681 agaaaaaaat actcaaacga attatgtcct tttaagagaa aaaatgattt ttgaagaata
    13741 tagcaggatg cgagcagtca gattagcccc aaaaaaaaat aagaccatag gaattaacaa
    13801 atctcttctg accaatacat tttttcctct ttattatcag ttcttttatg gtttaggtta
    13861 tgacattctt ttaagtgatg ataaggaaaa agaaggtatg gaaaggaagg gagcttcttt
    13921 ttgttatcct gttgaaatat cccatggggc tttggaaaat ctgattaaaa aggatccgga
    13981 tatttatttt cttcctcata taaaatctat agaagtaaaa aatggtttcc cagttagcgt
    14041 cacttgccct ttggtacagg gagaacctta ttatttaaaa gccagttttg atgagttggc
    14101 taaaaagcca gttttaagcc cagttttgga ttttgcaaaa ggctatgatg atttaatgga
    14161 cagctttata aaaatggcta aagatttaaa tgaagatgtc gaaattgcta aaaaatcttt
    14221 ccagattgcc gtgaacaatc aaatggactt ttattacaaa tgtaaggaga ttggaaagag
    14281 aatattatta gatttggaag atgaaaaaga taaattagcc tttgttttat ttggcagacc
    14341 ttataatgct tttagcagtt ggggcaatat gggcattcct aataaatttg ccacacggaa
    14401 ttatgtgatg attccctttg attttttacc ttctgatgga gaagaagcag gagacaatat
    14461 gtactgggca atgggacaac ttattttgaa agctgcccgg atggtaaaaa aacattcaca
    14521 attatttggg gtgtatatta cgaattttag ctgtggtcct gattcttttt tgaacggata
    14581 tttcagaagc attatggaca aaaagccttc tttggtttta gaacttgaca atcatacggc
    14641 agatgcgggc attgatacca ggatagaagc ttttattgat attgtaaagt cctataagca
    14701 gcttagtgat cataaaagtt cactcgaaca ggatgtcttt tatcctgcag agatagaaat
    14761 gaagaaaggc tttgcccagg tttatgattc cagtggtcaa agttattctc ttaatgatca
    14821 aagggtgcat gttcttatac cttctatggg agacatggct tctaagctat tggtagcaac
    14881 gtttaattat atagggattc aagccagtgc cctggaggaa ccgggggaag aagaattaaa
    14941 aattggaaga ggcctttctt cctgtaaaga atgtcttccg cttcagttaa caataggtag
    15001 tttaatgaaa tatttaaatg aaagaaaaaa tcaggatgaa ttattggtgt attttatgcc
    15061 ggatacttca ggtccttgta gatttggtca atacagtgtc ctgataaaaa attaattgct
    15121 gagcttaaaa taagagatct agcagtcttt tctcttaact ctgaaaatgg atatgccggc
    15181 tttgggatgg attttttgct tagaggctgg cattcggtga ttatttctga tgtattggaa
    15241 gatatcagga gtgccatatt ggttttagca aaagataaag aaaatggaat gaaaatatat
    15301 gaagaagtct ctcaggaaat tcttttaagt gcccgtaaag atagctggaa gaagttaaaa
    15361 gagacactgg aagcatctgc cttaaaatta aaaagcattg aaattaaaat gcctgttgaa
    15421 aaggctgtaa aagttgcttt agttggagaa atatatgtaa gaagagaccg tttttccaga
    15481 aagaagttag tcgaaaagtt agcccaaaag aatattatgg taaaagttgc tccggttgaa
    15541 gaatggatgt attatttaga ttatattcag aagcaccgtt ttactttgaa ttctactttc
    15601 agggacagaa tcagtacaag tattaagggt ttttttaaaa attattatga aaaaacgatt
    15661 aaacaaatct ttgctaaatc gggcttgtat gaatataaag taatagaagt gaataaaata
    15721 atttcaaacg taaaagattt aatctctcca gctttaacag gagaagcaat tttaactata
    15781 ggttcagcca ttactgaaat cgtcgatgaa gtatcagggg taatatctat tggacctttt
    15841 ggctgtatgc ccggcaggat agcagaagcg gttatcagtg aaaaaatgaa tacaaagaaa
    15901 ttgctcatat ctccaaacaa gcaattaata gagaaggtga tgaaaaagca tcctgctttg
    15961 ccttttttat cgattgaaac ggatggaaat gtatttccac aaatcattga ggcaaaactt
    16021 gagacttttt gtctgcaagt agaaagatta catcaaagca tagaaaaaat taaaaataat
    16081 attgaagaaa tcagttcata atactccccc ttcagggggt tattatttgt ctaaagaata
    16141 ataaaacgta taaactcgct tattgaagta aagattcatt ttgtgataaa atgtgtaagt
    16201 taacaagata gatttttaaa aaggaaagtg ggataaacgg tgattcaaaa aagagaagat
    16261 ataagaaata ttgcaataat tgcccatgtc gaccatggga aaaccagttt agtggatgaa
    16321 cttttaaaac aaagtggtat tttcagacag aatcaggaga ttgcagaaag agttttggat
    16381 tccaatgacc tggaaagaga aagaggcatc accattttgt caaagaatac tgcggttttt
    16441 tataaaaatg ttaaaataaa tattatagat acaccaggac atgctgactt tggaggagaa
    16501 gtagaacggg ttttaaagat ggttaatgga gttatactgg tggttgatgc ctttgaaggc
    16561 cctatgcctc aaacgaaatt tgtattaaaa aaggctttag agcttgaact tcctgtaatt
    16621 gtatgtatta ataaaataga cagaccagag gccaggccag aagaagtgat tgatgaaatt
    16681 cttgatttat tcattgaatt ggacgcggat gaaagccaat tggattgtcc ttttgtattt
    16741 gcttctgcaa agaatggtac ggcttctctg gatataaaca gtccgggaac agatatgcag
    16801 cctttatttg aaacaattat taagcatata cctgctcctg caggcagcga agatgaacct
    16861 ttacagttgc ttatttcaac catagactat aatgattatg taggaagaat aggaatcggt
    16921 aaggttgaaa gaggcagcat aaaagttaat caggaagcgg tcatagtcaa tgctattgat
    16981 gaaaataaaa ataagagagt aaaaatcaca aaaatttatc aatttgaagg tttaaaaaga
    17041 attcctgtag aaaatgccaa aataggagat attattgcta tatctggcat agaagatatc
    17101 aatattggcg atacagtttg tgatgtaaat tttcctgaac ctttgccttt tgtaaaaata
    17161 tcagagccaa ccattgccat gacttttttg gtaaatgaca gcccttttgc tggtcaggaa
    17221 gggaaatttg tgacttcaag gaatttgaga gaacgtttat tcaaagaact gcagaccgat
    17281 gtcagtctga gagtagaaga aaccagttct accgatgcct ttagggtatc cggaagaggt
    17341 gaattgcacc tttctatatt gatagaaacc atgagaaggg aaggttatga gtttcaggtt
    17401 tccagaccag aggttttata taaggaaatt gacggaaaac ttcacgagcc aatggaaatg
    17461 gttacggtag atgttcctga agaatttgta gggactgtta ttgaaaagtt agggagcaga
    17521 aaaggtgaaa tgattagtat gagctctgta aaaggcgctt acacccgctt agtattttcc
    17581 attccttcaa gaggattaat tggataccgt tcagagtttt taaccgatac aaagggaaat
    17641 ggaattatta atgcagtatt tgatggttat gcgccttata aaggggatat tgaaaaaagg
    17701 caatggggtt ctttaattgc ttttgagaca ggagaagctg tgacttatgg attatatcat
    17761 gcacaggaaa gaggaatgct ttttatagaa cctggaacga aagtttatga aggtatgatt
    17821 gtaggacaga atcctaaagg cgaagatatc gaagtgaatg tatgtaaaaa gaaacatgta
    17881 acgaatatga gggcagcggg ctcagacgaa gctttaaggc tatcgccgcc aaaaaagatg
    17941 agtttagaag aatgtctgga gtttatcgaa gacgatgaat tgctggaagt gacacctaaa
    18001 agcctgagga taagaaagaa aattttgaac agtggagagc gcaaaaaagc aagaaaaaaa
    18061 taggagtcta attgaggtgg attattatga agaaaaaaaa ttccggggga aaactggtta
    18121 agcaagcagc ggttttggct attgcaagtt taattgtccg cttgatgggt cttatttacc
    18181 gctggcctct taccaatatg attggcgatg atggcaatgg tctttatggt atagcattta
    18241 atatttattt attctttttt gtcatatcat cgggaggctt gcctgcggct atttctaaaa
    18301 tggtatcgga acggatggca ttaaaagaat ataaaagtgc tcataaggtt tttaagacgg
    18361 ctttaatgct ggctgcttca acaggtctta cttgttgttt aatcctttgg tttggggcac
    18421 aaccgattgc aaagctttta aataacaccc ggattgttta ttctatgaaa gctttagcac
    18481 caaccttatt gattgttgct attatggggg cttttagagg ctattatcaa ggtatgaata
    18541 ctatggtgcc tacagcgatt tcccaaatca ttgaacagct atttaatgca gtttttagta
    18601 ttgttttagc tgctgctttt cttaaaaaag gaatggaata tgcggcagcc ggtggaacga
    18661 tgggaacagg tattggtgcc ttggcagggc ttatatttct tattctttta tacatacttt
    18721 tgcgtccaag attgttaaaa agagtgaacc agcaaacgga tagtcatttc gatgaatcct
    18781 atcaggatat tttgaaaacg cttctttcaa tatccattcc tattattatt ggaacggcaa
    18841 tatttagtat tacgaatctg attgatgcaa aaatggtgat gtctatatta caatctttag
    18901 gaatgtctga aatggaagcc aatgagttat atggtcagct gacggggaaa tatgttactt
    18961 tgacaaatct gccagtttcc atttcttctg ctctggcagc tgcagttgtt cccagtattg
    19021 cagcttccat ggctttaaaa gaaagaaaag ttgtgatgaa taaagtgaat ttagctttaa
    19081 gggtttctat gattcttgca gcgccatcta tggttggttt gtttgtattg ggcgatccaa
    19141 ttcttaaaat gctctttccc aattaccccg gtgggggaga tttgcttaga attggttcaa
    19201 tagcaattat atttttatct gttgttcaag tttcaacagc tattttacag ggtgttggta
    19261 aagttatggt ccctgcaagg aatgctgcta tcgggacagc ttgtaaaata attattaatt
    19321 atttgctcat ttctgttcct ttgttaaata ttaaaggggc agtaattagt acagtcattt
    19381 gctatattat tatatccttt ttagatatga aggctgtaag gaaggttaca aaggttaaaa
    19441 ttgatatatt gggtacctat gtaaaaccta ttgcggcatc tgttgttatg gggatatttt
    19501 ccaggatatt ttataaactc tttttatggg gaagtcaaag taatactttt gctacgatgg
    19561 gttctattat cctttctatt gtggtgtact gcagtttctt aattgttatt ggagggataa
    19621 caaaggaaga tatcttttta cttcctaaag gagaattgat ttatagcaaa ttaacaaaaa
    19681 tgggcatgat gcaggaatga taatgactta ttctgactga ctacctgaac cagaatatgg
    19741 cgcggttatg aatgaataaa tcaatttttc atacagtgat tggtataaaa tgataaacaa
    19801 tggaaaagat ataaacaaat cactgtatgg aggtttaatg tgattatgca aagaaaaata
    19861 tggattttat ctgcctttgt ttttttaatc agttctgttt ttgcctatgc tttatatatg
    19921 catggatata ataaagaaaa ccgtaatctg gaccatggaa aaaaatttct gagtatatta
    19981 gtccggaaaa aggctctcag gcggccattc cggacaggac tgtccatgag aaagagttcg
    20041 ttgctgtaca aggaaagcag gacaaaatca attcatcaac tttgatgatt tatcaatatc
    20101 acgatgaggt caggaataaa gtgattgaag aaagagttaa agcaccctat tttcttatgg
    20161 atttaacaag ggatgagttg caggaacttt attccgactg gcaagttagt tctttttcca
    20221 gtgaaaaagt ggtgttaaga aaaaaaatcg attcaaaata cagtaatcaa tatgtgttag
    20281 gagtttataa cggttttgtg acgatttttt ctaataatga agaaggttat ccggaaataa
    20341 aagaaattac agaaaccccg atttcttctt tgcctctgga agaacagaat aaactggaaa
    20401 aagggataga aatctatggg gaagaagaat tgatccgaat gttagaagat tataccagct
    20461 agcatgagaa aatttttctc atgctatttt tatgagaaat ggagttaagt taacaaaagc
    20521 aatttagctt gatggaacga ttcttatatc ttaaaatatt aataaacagc caaatcaagt
    20581 tataaaaaat aaggagaaga tcatgagtaa aaagtctaag gtattaatta ttggtggtgg
    20641 cgctgctggc ttaatggctt cgattgctgc acagttaaat ggagctcagg ccgttatatt
    20701 agaaaggatg aaccgggtag ggaaaaaaat tttagcaaca ggtaatgggc gctgtaattt
    20761 gaccaatgtg tgtctggata ttaaaaatta tcattcgtca aatataaaat ttgttcatgg
    20821 gatacttagc cgctttagcg tagaacaaac tttagatttt tttaatcaga tgggtattga
    20881 atacaaagtt gaagacaaag gtaaagtcta tccattaagt gaacaggctt ccagtgtatt
    20941 agacctgtta agatatgaaa ttcaaaggtt gggtattcag gaaatttgtg aagcagaagt
    21001 ggttaaaata aaaaaagaca ggaagaaaaa caataatgct tttactttgt ttttaaaaga
    21061 tggtcggacc ataaccggcg acagggtgat tttggcgaca ggaggcaagt catcaccaaa
    21121 tcttggttct aacgggagtg gatatcaatt ggttattcct tttgggcata agttgattga
    21181 gccatttcct gccttagtcc agttgaatct tgaggcatgg tttttaaaaa gattaaaagg
    21241 ggtaaagttt aacgggaaag ctgctatatt ggataaggat aagtttttga ggacagaaga
    21301 aggggaaata ctttttacgg actacggtat ttcaggtcca cccattttac agttaagcag
    21361 aaagtatggc gagttgttgc ataaaaagaa taccagaccc ttaatttctt tagatttgta
    21421 tccggatttt tctaaagaag aattaaaaac gaagattttg agcagatttt attatcatcc
    21481 tgaaaaaaca atcgaatttg cttttgtagg catgatcaat aagcgactta tacctgttgt
    21541 tttaaaagaa gcgggtttaa atgatttaaa tatcatttgt aaagatataa aagataaaga
    21601 ggtagataat ttgattaatc tgtttaagga ctggagaatt ccagtcaaag ggactcagtc
    21661 gtggatgcat tcccaggtga ctgccggcgg aataagtctt gaagatattc atccggcaac
    21721 catggaatca aaactattac cgggacttta ttttgccgga gaggtcgtcg atgtggacgg
    21781 ggattgcgga ggctttaatc ttcaatgggc ctggtcttcg ggatatattg caggagaaag
    21841 tgcggcaagg actattggct gaccatacat aattttgctt taaattctta taatattagg
    21901 aacaagaata acgaaacgaa aaatataata cgaaaggatg cataagatgg atgcaatttt
    21961 ctcaataaat tcctttcaat atagctacat tgtcctgcca gtcattattt ttttatgcag
    22021 ggttgtagat gtaacgatgg gaactatccg tgtgattttt gtttctaagg gatatagagt
    22081 tggtgcagcc cttttaggct tttttgaagt ttttatctgg ataacggtca ttggggaaat
    22141 tatgacaaat gccaatagta ttttttgtta tttagcttat gcagcaggtt ttgctacagg
    22201 caattatgtc ggaataatat gtgagaacaa attatccatt ggtcttgtag tcgttaggat
    22261 tattacacaa aaaaattcag atgaacttat tgaatatctc aggaatgaaa attatggtgt
    22321 gacggtttta gaagccacag gttctaaagg aaaagtaaaa atcattttta cggtgattaa
    22381 gagaaaagat ctgaaagcgg ttattgagaa tattaataac tttaatccta aagcttttta
    22441 ttccgttgaa gatgtgagaa cagtgaatga aggtgttttc cctaaacgag attttgattt
    22501 aatgaaggct aaagccttaa caagaaaaga aaaataaaat aaaataaaaa ataaaagatg
    22561 ggctgttgta ttaacattta aaagcgtaat caacagcctt ttgttttttt acaaaaaaga
    22621 aaatgccttt tactggaaag tcagtttatt ttttgaagtt ataagtaatt aaaatgagta
    22681 atattattta aagaagataa aaatcagtgg atactatgag ttaaaaaact atatattgac
    22741 aaaagaagct tttctatttt taaatactgt aatttttgaa aagcccgcct tttttagcat
    22801 ctcataagca caatcgaaat gggaacagat atcttccttt cggtgggcat cagaacctac
    22861 ggtaataatt tcaccgccaa gttctttata cctttttact atagcaaact gaggactggg
    22921 atgtcctaag ccgtatctaa aacctgaagt gtttatttca attccttttc cagcttcaat
    22981 aatcgttttt aagatggtat caatgatatc tgaataatct tcataagaga gtattttatt
    23041 ttcataattg ccatagcggt taatatagtc taaatggccg tatacatcat aataattata
    23101 tttttttaca ttatctaata cacattcgaa atatcttaaa taggcatttt tttgttcttt
    23161 acctttaaaa aagtccccat tgtataagtc cagccggtca caggcatgca tggaacatat
    23221 gatgaattga aaatcgtatt tactaaaaaa atcatttaat tcattaacaa tatggggctg
    23281 ataaccaatt tctacaccta agatgatatt tattttatct ttgtattttt cctttatacg
    23341 gtcaaattca atcatatatt tatcataatc aatcataaaa ggaaactgag gatcaggata
    23401 gtcaaaatct acatggtctg tcagggcaat ttcttttaaa ccaagttcaa ttgctttttg
    23461 aatctgattt tccatacttt cctggcaatc actcgaaaaa gaagtatgaa catgatagtc
    23521 tgcaaaaaac attttatacc tccattttta ataaaaatat attgtataag ataagtgtat
    23581 cctaattata ctataaagta aaagataaaa atttatataa aataaataaa aatgtttgaa
    23641 aactgcagaa aaatgatata atataagtgt ctttttttct tattaaataa aaaaggagaa
    23701 tgataatgtc agaacaaaat tttaaaaacc atcaacatga tgatcaatgt gaatgtggtc
    23761 atgaagaaca ccatgatgaa gatgtaattt atttaacact agaggatgac acagaattaa
    23821 aatgccatgt attaggcttc tttgaactag ataataaaga ttacattgcc cttcttcctg
    23881 aaggcgaata tgaagtttta atttttaatt atgttgaaga tgaagaaggc attgaaatca
    23941 acaatattga tgatgatgaa gaatttgaaa gagtatccga ttattttatg aatgaattta
    24001 aagatgaact cgaaatgatg gcagatgact atgatttcga tgatgatttt gatgaagact
    24061 atgattttga tgaagaagat gagcaataaa taaaacgccg gtttccggcg ttttatttat
    24121 tttaaaggac gtataaacat ctgagtccaa tatttaactc cgcttttact aacagctaag
    24181 ccaacaccta tctcactata agatgtactt aaaatattac ttctgtgtcc cggtgaattc
    24241 atccaggcat tcataactgc tgaaggactt tgttggccct ttgcaatatt ttcaccagct
    24301 gaagaatagc ggatgccaaa gctttccatc atttggaaag gagaaccata agtcggggac
    24361 tgatgagaaa aatagttttt attaatcata tcctgggact tcattcttgc aactctagat
    24421 aattcccaat ttgctttaag aggcgaaaga ccatttttag ctctttcagc atttactaat
    24481 tttatgactt cattttccaa tgattttaca tcatcaatat taggaatgtt gagtttttga
    24541 ttgggataga ttaatgccgg attttttatt tgcgggtttg ctgcaatgat ttctgataaa
    24601 cctatttgat gttttacagc aattttccac atactatcgc caggctggac aatatatgtt
    24661 tttgtttgtg actgagcaaa agtaggtact gaaaaaatta taaaaacaaa taatgacaag
    24721 agaatttttc ttttcatatg aacacctcct ctattagttt gaaaaaaact acaataatta
    24781 tctattgtat tttatactat tattggaaca aataccctca acgatagcgt ttgtaaatga
    24841 ttaataataa atttaaaaat agacaagcat ttctttgact gtcagggaaa tatttgctat
    24901 tatttaatta tctaattatt attaataaaa ttaattaagt taattaattt tatttagggg
    24961 gttatttatg agagaacaag tattattcat gaaaaggacc atagaaaaca aagaagaact
    25021 cattgatgtt ttcagtaaga ataaaatcga actcattact tctcatagta ctgaagaaac
    25081 cctggatata cttgatgaaa aatttgataa aattgactta ttaattattg acatggaaga
    25141 agaccctata tttggattaa aaagcattaa aacaattaaa aaaataaatg attacaaata
    25201 tattcccatt gtcattattg caaacaaaaa agatgttatg aaaggtctga caatgggtgc
    25261 taaagagtat tttattccac cttatgacta tgagaaactc tgttcttttg tccagattat
    25321 ccttaaaaat aaacgagaaa atatgcattt atatacccct gagacctcta ttgatatgag
    25381 ttttgaacag tattttaaca gtgaaataaa aagggctgaa agaggaggat atgaattagc
    25441 catcctgata ctcaccattg tttcaaaaga ccataacctt tatactcagg aaagaacgat
    25501 tttaactatg attaatcaat tggtggattt ggtacgacaa aacttgaggg caacagatac
    25561 cattgtccga tataatagaa gtaacgtgat tgcttttctt ccttatacct ccagatttaa
    25621 tggagaaatc gtatatgaaa aaattttaga gattttcaac gaaaagattt tagccaatgt
    25681 agatagcaaa aataattttg aaatggttta tagcattgct tcctatcctc aggatggaga
    25741 tacagtagaa gatttattat ttaatgctga aagatttttg gaagataata aaacttttct
    25801 gacagggaaa taataaaaat aagtgttttt atttgtaaaa actattgatt tttaatagag
    25861 tacagtgtaa tatatcccat aagtaccgat tgatttattt tgttaaaaat gctgttttgt
    25921 agaatggagg ataggatgcc aattaaaatt attacagaca gtgcggcaga tttaccaaaa
    25981 gaaatcattg aacaatatga catagatgtt attccgcttt atgtttatca tgaagatgaa
    26041 gaatttttgg atggtcagac cttgcatcct gatgaattat attcaggaat gagggaggga
    26101 aagatatata aaacaggaca agtacctcct gaaactttta aaaggtcttt actgaatatg
    26161 ctcagaaaaa tcaggcttgt atttatgtag gtttttcttc agctttgtct ggcacttatc
    26221 aagcttctat gattgcaaaa gaagatgtcc tggaaatata tcctgatttt gatatcgagg
    26281 tcattgatac gaaatgtgct tcacttggct ttggtttagt agtctataaa gctgctcaat
    26341 taacaaagca aggaaaatcc aaaaaagaaa ttgtagaagc tgttcgatta tattcaaagc
    26401 atatggagca tgtttttacc gtcgatgatt tggaatattt atatagaggc gggagaatca
    26461 gcagaacctc agcggtttta ggtggcattt tacacataaa acccattatc aatgtagaag
    26521 atggaaaact tgttcctatt gaaaagactc gtggaagaaa aaaagctata agacgtctta
    26581 ttgagcttgt aggtgaaaga ggtaaaaatt tagaaaaaca gatccttgct attagtcatg
    26641 gggatattcc tgatgaggca caagaggtta aagaaatgtt gatggaaaaa tataaatgta
    26701 aagatgttat tattaataca ataggatgcg ttattggcgc ccatgtggga ccaggcactt
    26761 tagctttatt ctttcttaat gaagcattgc ctttttaaac ggtttttaac cgtttttttc
    26821 tttgaaatga taaaatatta gagtatgagg ggattatcga tggatgcaag aaaaaaatta
    26881 agagaagcta aaagaattgt tattaaaata ggcacatcat ctttaaccca tgaaaatggg
    26941 agaatatatt atggaaagat ggaacgttta gccagaattc tttctgattt aaaaaatgaa
    27001 ggaaaagaaa ttgttttggt ttcttccgga gcaattggca ttggttctga aagattagga
    27061 tggactcaaa aaccaaaaca aacagaaatg aaacaggctg cagcagcagt agggcaaggg
    27121 atactgatgc agacctatga gaaatttttt aatgaatata atcaaattgt agctcagatt
    27181 ttattgacga aggatgtttt agaggattca gtgaaaaaaa ccaatgcaca aaatactttt
    27241 aagcagcttt taaaaatggg ggttattcct attgtcaatg aaaatgatac tgtggcaacg
    27301 gaggaaatag aagaaagtgt atttggtgat aatgatacct tatctgccat ggtttctgtt
    27361 ttaattgatg ccgatttgtt aattatttta tcggatatag atggacttta tacaaaaaat
    27421 cccaaagatt atgctgatgg ggtattaatt gatactgtag aagaaattac gaaagaaatt
    27481 gaagagttag ctggtggtaa aggaagtagt tttggaactg gtggcatgat tacgaaaatt
    27541 tgtgctgcta aaattgccaa cagtaatggt gtagacatgg ttattgccaa tggtgatgat
    27601 ttgaagaata ttatcagaat tttaaatggc gaaagaatcg gaactttatt taagaaaaac
    27661 actgatataa ttccttatac cagtgttatt taaacaaatc cgtcatgctt tttatgtttt
    27721 ctaaagcaat atcagcttta atggtggtta aggttgaatc cttttgctct ataacatctt
    27781 ttaattcaat taagatttgg ttaaactgaa tagcgagagg ttcttttttt cagtgatatc
    27841 tgcctgaagt tttggccaaa tttcttctat ttcttttaag tctttatgga cgagatccca
    27901 ttcacccagt ttggaagcaa tttttatatc tctgataaga tattttagtg tgagaatttc
    27961 agaaggtgtt ttcactttga ataaatccga catataaggc aggtgctttg taatttgtac
    28021 agctgcctgg gctattttta attcagaatc cgaggcagtt aaaaggggat aattttctgg
    28081 taattcttct tcaggaaggg ttatttcttc ttgttttgtt tcattttttt ccccaggttt
    28141 ctcatcagag gaacttttac tttcttcttt agacttttct ccttccagtt gctgttgtaa
    28201 tttcaaactg gctcgtttgt attctaattc agcttttttc tgctttcttc catggcttgt
    28261 gcgatgacca agctggttaa ttggtttaaa tctttattaa acgcttcagc tttttctgca
    28321 gacatgcctt ttttataggc ttcgctaaaa aaactgttcc atgttgaatg aagggatttt
    28381 acttgttggg cagaactatt ccagtctttt ttgcttacgg ataacaatag ttcttctgtc
    28441 aggttttcaa tttcttcaaa agcttcgtaa gaattgcttt ctttactgga atttttattt
    28501 tttttcttga cttcttttac agctaaccat taaaataata catataaatg ccagtatata
    28561 gaataataaa tattttttca taataacctc cttcccatga atttatagat atacttaaga
    28621 gaacatggaa ttatatatat attattacat atatatctgt gtaaaataca attttaattc
    28681 tgatgttttt gaaatttgat ggaaaatgat atataatgta ataaaaaatg gacgataaat
    28741 caattgagct ttttaggcta atgatgagga tggtattaac cagccttaaa taataaaata
    28801 acaaacttag aaagtgaggg gtataatgaa ttattgccgt gaatgtggaa aaatccttga
    28861 tgagaaccaa aaagaatgtc ctttttgcgg tacttatctg ggagatcaaa ctgcaaagga
    28921 ggaaactggt ttaagcagta atcctttgaa tcaaaccaat ccaggtccat caacagataa
    28981 tagttttaac tatagcagtt ccggtccagg atcaagtcag gattatggat acaagacaac
    29041 aacagattac agttattctt ccatgggggg aacaggttct atgaataata atccttatag
    29101 tccaatggta acagaacctt tgaataactg ggtaaaagta atcttatcct ttgtttcatc
    29161 ctggattccc ttggttggaa ttatagccgg aatcattttt atggttaaag aagatcagga
    29221 tagaaaatct tttggtcaag cacttcttac catgagtatt gttgttttcg tattatgttg
    29281 catttgttgt gtgattttag cttcttctgg cgccttagca gaaatgaatc ctattattaa
    29341 atgaaatagg tgagttagat gagatttttt ttgtattttt taggccgttc ggtatgtcat
    29401 cagatacccg aacgttcttt ttttattcat aatattcaat ttcctttatg tgcaagatgt
    29461 acaggaattt atacggggat gtttatgggt tttttatata tcttaatcag aaaaagacat
    29521 aagggaaata agccaccttc cataaagtat gtaattctta tgattatttt tttactgcct
    29581 atgataattg atggcctgac ttcttacctt ggtttcagga gtacaaataa taatatccgt
    29641 attattacag gacttatgtt tggtgttatt atacctgttt ttattgtatt gataagtaat
    29701 tttgatgtcc aaaaacataa tacccttcca attattaaaa actatatgga tattttagga
    29761 atgctgctta caataatcct tccatttttt cttatccatt ttgaacatgt aattatttgg
    29821 tggttgattg cgtctgtatc tgtattgatt attccattta tttatgtgaa aattttttat
    29881 ataatattaa aaaatctttt tagttccctt aagaaaaaac aggtctattt tgtggcatca
    29941 ctattttact taatttttat ggtcagtttg tcattaatga ataaattttt cctggcaaag
    30001 ttcagatagg agaatttgga tggatgaaaa aaagaactta cggactgtat ttttaaaaaa
    30061 aagaaatgag ctctctttcg aagaagttga gaaaaaaagc gatagaatat atagaaaatt
    30121 tataaattcc aatgattatt ttcattgtaa agagctgttt acttatgtaa gtatgaaaaa
    30181 agaagtcagt acggttaaaa tcataaaaaa ggcactggaa gatggaaaaa cagttgccgt
    30241 ccccaaagta gatgcgataa aaagagaaat gtttttttct atgatttatt cgctagatga
    30301 attagagaca ggtcattttg gtgtccttga gccaaaaaaa gaatgtgtac gtcctgtgga
    30361 ggcaagtcag tatacgctta tattagtccc gggagttgta tttgatttga acaaaaatag
    30421 aataggctat ggtggtggtt attacgacag ctacctgcga aaatataaag ataaagtaat
    30481 gaagacaatt ggtctggcct ataactttca acttatagac agaatacctg ctcaaaaaca
    30541 tgatattccc ctggatttaa ttgttacgga taaagactgg ctatattagt tgtattttat
    30601 ttgcaaatcg tttataatca aagaacagat gttcttctat tatttgtgaa aggatgagtt
    30661 ttttgataaa ctatttaaaa ggaaccttag agtatgctga tgaaggttct ttaattatag
    30721 aagtaaacgg agtaggctat aaactggctg ttcctctttc tgcgatggaa aaacttcctc
    30781 ctttgggcgg tgaattaaaa atttatactt atcttcaggt taaagaagat gaattgtgct
    30841 tatatggttt tttatccaaa gaagaacaaa gtgtctttga aaagttaata acggtcagcg
    30901 gaataggtcc taaaggagca atcaatattt tatccgtttt gtctccaatg gatatatata
    30961 tggccattgt atctgcagat ataaaattgt tgtcgagtgt ctcaggtatt ggtaaaaaaa
    31021 cggcacaaag gattgtttta gagttaaaag ataaaatggg aaaccttcct gtaatggatt
    31081 taaatgaatt acaaacagta tctttggaaa ataatggcct tgtagaagat gctttatctg
    31141 ccttaatggc tttaggttac aaaagagcgg aagctttgca ggcagtcaga caagtgtata
    31201 ctgagggaat ggaacttgaa gcagttataa aagcttcatt aaagaaatta tcaacaatat
    31261 aatgaaaaca ggtgaaatct atgaaagatc ggattattag tgctgaactt cgtgctgaag
    31321 attttgaaat ggaagcaggt atccgtccga agacttttga tgattatatt ggtcaggaaa
    31381 aagtaaaaaa taatttaaaa gtatttatta aagcagccat gaaccggaaa gaaactctgg
    31441 accatgtctt gctttatgga ccaccagggc tgggaaaaac gactttggct aatattattg
    31501 ccaatgaaat gaaggtaaat attaagatta cctccggacc tgctattgaa aaacctggtg
    31561 atatggcagc aattcttaat aatttaaatg aaggggattt attatttatc gatgaaatcc
    31621 atcgtttgaa cagaagtatt gaagagattc tttatcctgc tatggaggat tatgttattg
    31681 atattgttat aggcaaaggt cccagtgccc gatctataag attagagttg cctaaattta
    31741 ctttagtagg tgctactaca agggcaggtt tgctcacagc accgctcagg gacagatttg
    31801 gagttattca aagactggaa ttttataccc cttcagaatt agcggaaatt gtcagtcgat
    31861 ctgctaaagt attaggtatc gaaatagact cttcaggagc atatgaaatt gctaaaagat
    31921 caagaggcac accaagaata gctaatagac ttttaaagag agtcagagac tttgcagaag
    31981 taaaatataa cagttttatt gatgaaaaag tagcttcttt agctttggaa gcattagaag
    32041 tagataagat gggtttagac aggatagaca gaaaaatgat tatgacgatg attgaaaaat
    32101 ttggtggagg tcctgttggt attgatactt tagcagcagc tattggagaa gaaaatgata
    32161 ccatagaaga tgtatatgaa ccctatttga tacaattagg ttttatcaac agaactccaa
    32221 gaggaagagt tgttacggat tttgcgtata aacattttgg aatagaacaa ttgaaactgt
    32281 aataattgaa gagaagaaaa gtggctcaac aagcggcttt cttctctttt taattgacaa
    32341 ctgataatat aatgatataa tgataataga gtagaactct atcctctata gatatcttgt
    32401 atagggttaa atgattaaat tgtttaaaac taaagaaaga ggggttgtat gggtggtaaa
    32461 aaagccttaa atgaagaacg tctgatggaa ttggcaaaaa aggtttcaga aaatgaacag
    32521 ttgattaatt ttgaaacgtt taatgaagag atgacaattt cgcaagttgt ccgttttttt
    32581 aagagaaatg gtaaaaattt tactaagacc atgattcaaa attacgttag agtaggtgtt
    32641 attcccccac cagttgataa aagatattat actaagaatc atttaatttt gcttactctt
    32701 gtagataatt tgaaactgat ttattctttg gatgaaataa aaacgctctt tggtcctatt
    32761 ttaaaaaata ccacaagctt tgatgatgat atgataaaag tgtcggattt atatagtgat
    32821 tatattcacc ttcatgcgaa agccttaaaa gactggaaac aatatctgcc cagtactcta
    32881 gaagaagtga acaaaaagct ggaagattat catattaatg aaaatgaaaa agatatcgct
    32941 tctgtgttta tgcttgtact taccattatg gctgaaacga tagctttgaa aaagttaatt
    33001 gatttaatat ctcaggaata tttaacaaat aatgaataat aatatattgt ttatctgttt
    33061 taatatgtta taatacttat aatagtactt cttttatact aaggagcggt catatggaaa
    33121 aaaagaataa aatcgaagtc attataaacg gcaaagtata taccttggta ggcaatgaat
    33181 cagaagaata tattcaaaga gtagctttat atattgataa aaaaatgaat gagattaaga
    33241 atgctgaagc atcaaaaacc ctgagtacaa gtatgattgc tatattgact tccataaatg
    33301 tggcggatga tttatttaag ttatctgagt caatgaatca tttagaaaaa cgtatgaagg
    33361 atttagaaaa ggtattagaa gataaaaaca agcaaattaa atcttatcaa agtgaattgg
    33421 ctaatttaag aaaagaaaat cgggaattaa gggatactat cagtaaacag gaagttgaaa
    33481 ttaaaaaaag tcaaatggag cttgaagaat atatcaatat atttgattca aatgtaaata
    33541 acaggatttc aggtgacaag taagtaattg aatagaataa acttaaaagg ggagtaatat
    33601 aattattaac tccccttttc tctatactaa atttttttaa cagggtgata gaattggata
    33661 aacgaagtgt agaacttctt gccccagtgg gaacgaaaga aagtgtaaaa gctgcggtca
    33721 gcaacggctg tgatgccatt tatctggggg ggaaagcttt tagtgcaaga caatatgctg
    33781 acaattttac gattgaagag ctaaaggaaa ttatagatta ctgtcatctc aggaatgtga
    33841 aagtctatat tacggttaat accttgtata agcaaaaaga aatggatgat ttatttgatt
    33901 ttttgaacca gttatatcaa ttgggagtgg acgcttttat tgttcaggat ataggtactg
    33961 cggtggaaat aaaaaaagta tttccaaaga taagtctgca tgccagtact caaatgacca
    34021 ttcataattt agaaggtgta aagtttttag agagtcttcg ctttgacaga gttgtattaa
    34081 gcagggaact tagtttagaa gaaatatctt atataactga aagaacaaat ctagaaattg
    34141 aagtgtttgt tcatggtgcc ttatgcttta gttattcagg ccagtgttta atgagcagta
    34201 tgattggcgg cagaagcgga aacaggggaa gatgtgccca gccctgcagg ctcccttacc
    34261 gtttaatggg tgaaaatcat cgtgaaatga tgtcaggcta tcttttaagt cccaaggata
    34321 ttcagacctt agattatatt cctcaaatga ttgaagcagg aataaaatct tttaagatag
    34381 aaggcagaat gaaacggccg gaatatgtag gagcagtagc aggtatctat aggaaatata
    34441 ttgatttata tttcgaaaag gctgatgatt acttagtcga tgagcaggat ataaaaatcc
    34501 ttgcccagct ctttaacaga ggtggttttt caaagggcta tcttttttct caagggtcta
    34561 aggaaatgat gagttttgaa aatcctaaac attggggaat ttatgcagga aaggttaagg
    34621 attatgatgt aagaacaaaa aatgcgttat cgatacaact gtggatttaa aagctggtga
    34681 tggcattgaa atatggagaa aagaaaaaga aaacacaggt gttgaaattt caaaagcaag
    34741 taaggcagga gaaaagataa gtatatattt aaatgagcca gtgaaaaaag gcgatttagt
    34801 atataaaacc aaagacaagg aattgttgga acagatacaa aagacctact ctaaagatat
    34861 tcgtaaatat aatatatatg gaaatttaag attcaaaaaa catatgcctt tattgttaca
    34921 attatgggat gatcaaggta acttttttga attagcaggt cctgtggttg aagaggcaaa
    34981 gaatcagcca ttaaccgaag aaagaataaa agctcaaatc agcaagacag gaaatacacc
    35041 ttttatattt aaatctataa atatagattg tgatcaggat gcctatttag cgataagtga
    35101 attaaacttg atccggagaa aagctattga agcatttgca aatattatca tagaaaagtc
    35161 cagaagagat acatctttaa acaaacgaaa tttaatggtc aatataaaag aaagtataat
    35221 gaatccgaat aaagtgttta atgtcttatt aaaaaatctt gaagacatag aatatgctct
    35281 tcatccaaaa gtaaaacgaa tttatatgga tttaaatcat tataccaatg cagacattga
    35341 aaaagcagcc aggctttgtc acaaacacaa taaagcatta tttttagctt tgcccaggat
    35401 cgatttaaat tacaaaaaag aaaaaatatg gtataattta gaaaacagtt ctatagatgg
    35461 atatttatta agaacttatg gacaagtgta tcagctaaaa aattcaaaca aggaaaaaat
    35521 cctggattat acttttaata tttttaatca attatctgtt gattattggt taaatcatgg
    35581 catgaaagct ttgacgcttt ctccggaact caattatgat gaattaaaag ttttttacgg
    35641 aaaaggtctg gaaattatag gatatggtta tttacctctt atggttacta aacagtgcgt
    35701 tataggcaat actataggca agcgttcatc ttcttgccct tgtcattgta aagggaaaaa
    35761 agaaagatac aagcttatgg accgtaagaa cgaagctttt ccgatagaac aagattgttc
    35821 ttcttgtttg atgatgattt acaatagcaa gcctctttta cttttaaaag atttagataa
    35881 aattttatct ctgccgatca gttctttgcg cttagatttt tatttggaag aaaaaaatga
    35941 aataaaagac attattgaaa gttatattga caagccagaa atttatttta aaaaatataa
    36001 agataaggaa tatacgaaag gtcattattt cagaggagta gagtaggtga aaagacatgt
    36061 taaaggagtc catcattgat atcttaatac tcatatccag atatgctttt gttttgttta
    36121 tttttatttt tttatggtcg ggttataagg caattatttt tgaaaaagaa aatgaaacag
    36181 gtgaatttat aaataagatt tatatgcagc ggtttatgat tgtattgacg catgttttag
    36241 cttttgtcat attaatgatg acttccaaag attctgttca aatcattgaa attatgaaat
    36301 taagtgcttt tggtcttgca tttattattc ttttcatgat ttttacttct ctcatttata
    36361 aaaaatccga tcctgtgata tggaattgta tacttttttt aatggttgta ggactcatta
    36421 tgctggaaag aatagatcac gatttggcta aaaaacaaat tatttggttt ttggcaggaa
    36481 cctttgtaat gatttttatt ccccttgtct ttaaaattgt acccaggttt gaagctttca
    36541 aatatctcta tttaattctt ggttggatat ttattctttc tccttttatt ctgggaaaag
    36601 aaagatatgg tgcttacaac tggatttata tcgggaattt tgcttttcag ccttcagaaa
    36661 tcgtaaagtt cttatttata ttttatttag catctgcatt aagagcttat cattctataa
    36721 aagatttaat tcttccaagc tcgatggctg gtggttatgt actggtgttg gtactgcaaa
    36781 gagatttagg aggagccctg atatttttcc ttactttttt aatattggtt tatgtccgta
    36841 cttccagttc cttgctgttt tttggtggct taggaggcgc ttctttggct tcagttttag
    36901 cttataaatt attcagccat gtacaggtca gggttcaggc ctggttaaat ccctggaaag
    36961 aaattgatac gaaaggctat cagattactc agtctctttt tgccataggg acctggggct
    37021 ggttaggcag tggtctgacg gctggatatc ctaaacatat tcctgttgta gaaagtgatt
    37081 ttattttttc ggctatttgt gaggaatttg gaaatctttt tagcataggc attatcctgg
    37141 ttttcttgtt aatcatttta aggggtactt taattgccct caaatctaac agaatttttt
    37201 attctttgct tgcaatgggt gctgtaagcc taattgctat tcagactttt ttgattattg
    37261 gcggagtcat taaaatgatt cctttaaccg gagtgactct cccttttatc agttacgggg
    37321 gttcttctgt tattgtcagt atcatgataa caggcattct tcaatggata caaagctatt
    37381 atgcgaccag agaggaagtg ttggaatacg atgattcctt ataaaaaaag cattaaaaaa
    37441 gtcttttggc tctatgcttt aatgttttgt gccttaattg gatatttact taaatttttg
    37501 atggtggaca gttccagtgt tattgtgaat acatataatc caagattaag tgctttagaa
    37561 aagaatatac accggggaga aatccgcgac agagatgggg aagttcttgc cagaacggtc
    37621 gaaaatggtg atcgtttaat cagagaatat cccaaaggca gggattttgc tcatgtagtc
    37681 ggctatgtac aaaaagggaa aacgggtgta gaagcttatg gaaattttat acttctggaa
    37741 gtcagtgacc ggttcattca aagagtaaat caagtattta ctggagataa gttaaaagga
    37801 aataatatta ttttaacttt ggatgctgat attcagcata aagcaaggga attgctgaaa
    37861 ggcaggaaag gtgccattgt ggttatggaa ccatctacag gtaaaatcct tgctatggta
    37921 tcttatccgg attttaatcc taatgaaatt gatgaaaatt gggcaaaatt aaatcaagat
    37981 gaagaaaata gtcctttact gaacagagca agtcagggtt tatatccacc agggtccgta
    38041 tataaaattg taacggctgc agcaaccata gaaaatatgg aaaactggca ggattttcat
    38101 tatcgatgta gtggggaagc aaatttcaag ggcaattcta ttcgctgtta taattcccag
    38161 gcccatgggg aagtagatct ggaaaaagct tttacggtct cctgtaatac tgctttttct
    38221 cagcttggtg tggatttggg aggcgaaaaa atgcagctca tttctgaaag agttttattt
    38281 aatcgagatt tgccttatga attagaatat agtaaaggtc gctttgcttt aaccaggaat
    38341 tctgaggaaa aagaaattat tgaaacagct attggtcagg gaaaaacctt agtttctcct
    38401 ttacatatgg ccatgattac ttctgccatt gctaatggtg gaattttaat gaagccgtat
    38461 attattgacc atgtagaaac aaactatgga agagaaaaaa acaaacaaat cccgcaaaaa
    38521 tatgatgttt tatttagtcc ggaagttgcc agtcagttga ctgaaatgat ggtaagagtt
    38581 gtaaatgaag ggacgggtag tcaggcaaag attaaaaatg ttactgttgc tgggaaaact
    38641 ggaacggctc aaaatgcttc aggagaagat catgcatggt ttgttggttt tgctcctgca
    38701 gaaaaaccag aagttgttgt tgctgttatt attgaaaacg ggggatcagg gggaaggatt
    38761 gcaggtccta ttgcaaaaga gttgatgcaa atggttatta atcaataaga aggtgacctt
    38821 ggtgctgact ttatttcaat tggcattggc ccctgtgatt attacatgtt tttatattta
    38881 tatccgtgat aaatatgaaa aagagccctt gcgcttattg cttttaggac tgttttttgg
    38941 cttattaatt tctactccaa ttatggcggt agaaatctta ttgaccaata taacacctga
    39001 gggtaatcat ttaagagaaa ctctgtttac tgcctttgtt gttgctgccg gaacggaaga
    39061 attttttaaa tatctggttt tatatttttt gatatggaaa aatagaaatt ttaacgagcc
    39121 ttttgatgga attgtatatg ccgtatttat ttctttgggt tttgctggtc tggaaaatat
    39181 tctttatgtt ttaaatcctt atttaggagg ttttcaaaca gctgcagcca gggctgtttt
    39241 ttctgtacca gggcatgcct tgtttggtgt ttctatgggt tattattatg ccatagcaaa
    39301 atttaatcaa aataaaaaat caatgcttat tacggctttt tttgtaccct ggctgtttca
    39361 tggtatatat gactttattc tcattttaaa taattcttta ttaatgattt tattcgttcc
    39421 ttttgtgtta tatctgtgga ttcagggaat gcgaa
//
LOCUS       DAOSUD010000010        38398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_738_length_38398_cov_22.897113, whole genome shotgun sequence.
ACCESSION   DAOSUD010000010 DAOSUD010000000
VERSION     DAOSUD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 38398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38398
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_738_length_38398_cov_22.897113"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..1313
                     /locus_tag="PLG49_02575"
     CDS             585..1313
                     /locus_tag="PLG49_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ16230.1"
                     /translation="MKLQRLLSYVRRAVDDYNMIEDGDKIAIGISAGKDSLILLLALR
                     SLQRFYPKKFELEAVTISLGFDNFDLTGVQKMCDEIGVRYTVKETDIAQIVFHERKEK
                     NPCSLCAKMRKGALNDVAKSLGCNKVALGHNKDDVIQTLFLSLFYEGRIYTFPPVSYL
                     DRSDLHTIRPLIYVPEKEIVGFARKEGLPVLKSPCPADGNTKREQVKNLIADLRNQYD
                     NLDNHIFGAIKRSAINGWNLEKHL"
     gene            complement(1310..2023)
                     /locus_tag="PLG49_02580"
     CDS             complement(1310..2023)
                     /locus_tag="PLG49_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOQ16231.1"
                     /translation="MKVICEIGIQTLLAFFSIWFITRILGRKQIAQLTVYEYINGITF
                     GSIAATLATDLNQRTWHHLIGLFLFGILTWGMSYISLKNDGINQLLQGEPVIVIEDGK
                     ILEKNLKRCLYSINDLLEQLRLEKIFDIKEVKYAIVETNGALSVMKYEHLEVPTVQDL
                     GLVTSPKAPFCSIILDGKIIKDNMRLMKIDEDWLFMQLKAKGIQDIKEVMYAAVNQQK
                     EIYIDVYKDYAGGGEKTPL"
     gene            2303..3373
                     /locus_tag="PLG49_02585"
     CDS             2303..3373
                     /locus_tag="PLG49_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ16232.1"
                     /translation="MAEINYHEQEKQKNTLRLRTLLKSLPPFAFEFFRGIEPTTSPRT
                     RIAYAYDLRIFFEYILENHPKLKNKVINELEIKDLEEITPDHIEMFLEYLNYYSRPDP
                     NNPDKIVEYQNDERAKSRKLAAIRTFYLYFYKRRKISTNPPSLVESPKIHEKNIIRLE
                     VDEVARLLDEVESGENLTDTQKKYHHYTRLRDLALITLLLGTGIRVSECVGLNISDID
                     FDVNGLKIIRKGGNEMVIYFGDEVEEALRNYLEERLKKQPKPGHEDALFLSLQNRRLT
                     VRSVQNLVKKYASLVTKLKNISPHKLRSTYGTNLYRETGDIYLVADVLGHKDVNTTRR
                     HYAQIDDDRRRKAAKVVKLRKI"
     gene            complement(3382..3681)
                     /locus_tag="PLG49_02590"
     CDS             complement(3382..3681)
                     /locus_tag="PLG49_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ16233.1"
                     /translation="MQKKKRFRLFLLLAFAISLIQLFSILSTYCSTGHESFNNHLVKK
                     YEEVMIHRGDSLWKLAQKYKPEDKSTKEYVKLIKEFNRLESDRLYAGDTIIIPIY"
     gene            3937..4548
                     /gene="lexA"
                     /locus_tag="PLG49_02595"
     CDS             3937..4548
                     /gene="lexA"
                     /locus_tag="PLG49_02595"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOQ16234.1"
                     /translation="MSNKVGGKQLEILNYLKKEIKEKGYPPSVREICDAVDLKSTSTV
                     HGHLNRLEQKGLIRRDPTKPRAIEILDESFYPMRQELVNVPIIGKVTAGQPILAVENI
                     EDYFPLPVDYVKNDTVYMLHVQGDSMIEAGIFDKDLILVRKQSTAENGDIVVALIDEE
                     ATVKRFFREKDHIRLQPENPAMSPIVVRDVTILGKVIGLFRKF"
     gene            4569..6515
                     /locus_tag="PLG49_02600"
     CDS             4569..6515
                     /locus_tag="PLG49_02600"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOQ16235.1"
                     /translation="MENGFIRVGAAVPKIKLADCKSNSEEIIRLIEEAQNNLVQVLVF
                     PELSLTGYSCGDLFYQDILLEEAQNGLQKILDYTADKDILILVGMPLSVDNQLFNCAV
                     LLLKGNILGVVAKTSIPNHNEFYEKRWFSSGNHAVSKEVNLCGQKVPFGNDLLFQCSN
                     INHLCIGVEICQDLWTTVPPSSLQSMQGATLILNPSASDEYVGKRLYRNHLVLQQSAK
                     CMVGYIYASCGYGESTSDLVYSGHSMISENGTLLEEALPFSHDSYLIFTELDLLRIVS
                     ERKRAPVSGDLMGKNINQFISFRKVYFELNQNKIPTLSRKIEPHPFVPQDPQTREFYC
                     REIFAIQTNGLKKRVEHTGADKLILGISGGLDSTLALLVCVETLDCLKRDRKDIIGIT
                     MPGFGTSGRTYENAIQLMKNLGISMREISIKEACIQHFKDIDHDEKLYDSTYENAQAR
                     ERTQILMDIANKENGLVIGTGDLSELALGWATYNGDHMSMYAVNTGVPKTLLSPLIAW
                     ISNMPKYHNIKTITEDIIHTPVSPELLPPDQNGEINQKTENILGPYELHDFFLYYMLH
                     FGFRPSRILYLAETAFKDKYSRESIIQCLKIFYKRFFIHQFKRSCLPDGPKVCSVSLS
                     PRGDFRMPSDASYRLWLDEIELLE"
     gene            complement(6552..7838)
                     /locus_tag="PLG49_02605"
     CDS             complement(6552..7838)
                     /locus_tag="PLG49_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOQ16236.1"
                     /translation="MHEFVDEILRRNFGLSNEVIQFCKKIEKEIEPYFNEVDHIAEYN
                     QLKILAAMQKNHLSDIHFAATNGYGYNDLGRDTVENIYAEVFKAESGLVRPQLVSGTH
                     ALTVALFGNLRPGDELLSPIGKPYDTLEGVIGIRKMKGSLKEYGVTYKQVDLLEDGFD
                     YKGIENAITPKTKMVTIQRSKGYLWRPTLSVQQIKELIAFIKKIKNDVICMVDNCYGE
                     FVDYIEPSEVGADLVVGSLIKNPGGGIAPIGGYIVGKEEYVENAAIRLTAPGLGKEVG
                     ASLGLNQPILQGLFFAPQVVSGSLKGAIFAARIFEKLDFTVSPASCEKRNDIIQAIQM
                     KKPENVIAFCQGIQKGSPVDSFVIPEPSDMPGYDSQVIMAAGAFVQGSSIELSADAPM
                     KEPYTVFLQGGLSWHHAKLGIMIALQNMMDKKLIHV"
     gene            complement(7854..8096)
                     /gene="hfq"
                     /locus_tag="PLG49_02610"
     CDS             complement(7854..8096)
                     /gene="hfq"
                     /locus_tag="PLG49_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOQ16237.1"
                     /translation="MSKTINLQDVFLNQVRKEKMTVTVFLTNGFQLKGVVKGFDNFIV
                     MLESDGKQQMIYKHAISTIIPAKPVYYNVNDKNEGE"
     gene            complement(8140..9090)
                     /gene="miaA"
                     /locus_tag="PLG49_02615"
     CDS             complement(8140..9090)
                     /gene="miaA"
                     /locus_tag="PLG49_02615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ16238.1"
                     /translation="MKKPLIVIAGPTACGKTNLSIQLAKKIDAEIISADSMQVYKYMD
                     IGTAKPTKEEMENIPHYLIDEIEPDEEFNVAIFQEKAKKYLEMIYNKNKIPILVGGTG
                     FYIQALVYDINFMETNNDYEYRSYLEKLAKEKGNQYIHEILKKVDPVSALKIHPNNVK
                     RVIRALEYYKQTNKPISQHNEEEKNKKVSPYNTAFFCLNMKRETLYKRIEQRVDKMIE
                     AGLIEEVQALLKKGYSPDLVSMQGLGYKEIVPYLQGNISLEEAIYILKRDTRHFAKRQ
                     LTWFKRQENPYWINIDDFGFDMDIILAKMLNYIEEIGIII"
     gene            complement(9103..10971)
                     /gene="mutL"
                     /locus_tag="PLG49_02620"
     CDS             complement(9103..10971)
                     /gene="mutL"
                     /locus_tag="PLG49_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOQ16239.1"
                     /translation="MDPIRRLDDYTINKIAAGEVVERPASVVKELVENSIDAQASAIT
                     VEIKDGGISLIRVTDNGIGIPKEDVTTALLRHTTSKITAVEDLDKIESLGFRGEALAS
                     IAAVSQLEIITKAYGEVTGKRVECYGGKIITEEEVGCPEGTTIIMRNLFYNIPARKKF
                     LKKPATESSYISDMVYKIALGHPEVSFKFINNNSVVFHTSGNNELKNAVFNVYGKEVV
                     KHMLSIEETEQDLKLIGLIGKPSLNRANRTYENFYINGRYIKSKILENAVEDAYNTLL
                     PINKFPVVVLHIFIPANKIDVNVHPTKMEVRFKDEEEIYQFIYNGIHKNLFKEDLIPQ
                     VNLDNKKEKLFNPEPVQGRLPEPFEIKRKNLEYKDISIHPVFSPEDKYIKVKESNQEL
                     NFSHKENLYENKPCGTEKKEACTIFEKEKVHKEDKAPSFRHYKIIGQVFQTYWLVEQD
                     NSLYIVDQHAAHERILYEKFIHDFKQGKVYSQPLLEPVVFEVTPKEKEIVEDNISLFK
                     KFGFEIEEFGESAYLVRELPILFEGPVDSSFFLEIIDTLNDERIKNLYDMKLNHIALL
                     SCKGAVKAKDQLSTVEAKALIEELLNLDNPFTCPHGRPTIISMSQYELEKKFKRVQ"
     gene            complement(10984..13605)
                     /gene="mutS"
                     /locus_tag="PLG49_02625"
     CDS             complement(10984..13605)
                     /gene="mutS"
                     /locus_tag="PLG49_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOQ16240.1"
                     /translation="MLQLTPMMQQYLEIKKKVSDCLLFFRLGDFYEMFFEDALTASKE
                     LEITLTGRDCGLDERAPMCGVPYHSAENYINKLIEKGYKVAICEQIEDPRTAKGIVKR
                     DITRIITPGTQISSNVLDEGKNNYLLSIFASKDGYGLGVVDITTGEFQVTEIKEVSNQ
                     KKLLDEIAKYNPAELLSNSEFNKSADLIEEIKNRFHTFINEYGDWAFDYDSSYKILSE
                     HFKVHSLDGLGLKEFRLGICACGALLQYLRETQKVNLSHVTEITPYYIEEFMILDLSS
                     RRNLELTETLREKSRKGSLLWVLDKTKTAMGARLLRKWLEQPLLDVNEIHKRLDGVEE
                     FKNNPILREELKELLNGIYDLERLMTKVIYGTVNGRDLIALKNSISMIPHIKSLLNEC
                     QAAYQKEIYDSMDSLEDIYELIQQSIVDEAPISVREGGIIKTGYHSEIDKLRRAKTEG
                     KQWIAALENEEREKTGIKNLRIKFNKVFGYFIEVTKSNIDSVPDRYIRKQTLTNAERY
                     ITAELKELEETVLGAEEKLIELEYDIFVQIRERIEKEVERIQKTARLIAIVDVLQSLA
                     EVAEKQNYCKPEVNQGDSIFIEDGRHPVVEKMMPLEQFIPNDTFLDEKDNRLAIVTGP
                     NMAGKSTYMRQVALIVLMAQIGSFVPAKTARIGVVDRIFTRVGASDDLASGQSTFMVE
                     MTEVANILNNATSKSLLILDEIGRGTSTFDGLSIAWAVIEYISDKEKIGAKTLFATHY
                     HELTELEGKLSGVKNYCIDVLEKGDNIIFLRKIKRGGANHSYGIQVANLAGLPNEVIE
                     RAKVILSQLTQADITKNAKNIFNEIQVIQMDLFSMEQNEFKELIKEIANLNLSEISVG
                     QAIDILLDLNKKAKSLL"
     gene            complement(13636..15069)
                     /gene="miaB"
                     /locus_tag="PLG49_02630"
     CDS             complement(13636..15069)
                     /gene="miaB"
                     /locus_tag="PLG49_02630"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOQ16241.1"
                     /translation="MSKRRTPSMMTTEEAQRQETYIKQIRMQNDLEKSKTSKQKHYYI
                     ATFGCQMNAHDSEKLAGMLEQMGYQKTDQENEADFILYNTCCVRENAEQKVYGKLGYL
                     KYLKDMNKDLIIAICGCMMQQDTVLNIIKSKYPHVDIIFGTFNIYKLPELLQTYLETK
                     ETVINIWNEHKDIVEDLPSIRKYKFKASVNIMYGCNNFCTYCIVPYVRGRERSRQPED
                     ILNEIKELVADGVKEIMLLGQNVNSYGKTLKDGISFAQLLRKVNEIEGLKRIRFMTSH
                     PKDLSDELILAMRDCDKVCKSLHLPFQAGSTEILKKMNRKYTKEQYLELAEKIQNTIP
                     DIALTTDIIVGFPGENEEDFQDTLDVVRKVRFSGAYTFIYSKRTGTPAATMEGEVPEE
                     ICQRRFNELQEVLQPIVYEKSQEFYGKTVEILVEEKSKNKDSYLTGRTDSGHLVHFEG
                     DTSLIGQFVNVEIIEAKTYYLIGKLAN"
     gene            complement(15188..15535)
                     /locus_tag="PLG49_02635"
     CDS             complement(15188..15535)
                     /locus_tag="PLG49_02635"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOQ16242.1"
                     /translation="MSEFSKEIFINYTKKYFYFIAVFNFIDMILTLYATTNGYGVEIN
                     PFLARLLHNPPLFIIIKGILPSLLLFLVFNRLKKGSLMEIKKSIFYLKLCSYWYMVVI
                     ASHFVWIYYSLVF"
     gene            complement(15616..15819)
                     /locus_tag="PLG49_02640"
     CDS             complement(15616..15819)
                     /locus_tag="PLG49_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16243.1"
                     /translation="MNNNQFEKLKEKYKEGFRCIRSDESNGELTVHLKNFYNEKIDSL
                     KSDNPEEIRQILDYIQSVTEEFQ"
     gene            15995..16639
                     /locus_tag="PLG49_02645"
     CDS             15995..16639
                     /locus_tag="PLG49_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOQ16244.1"
                     /translation="MYVRNRMNTNVVTVTPDTPVTTVFNLMMEHGYSQFPVVEGNELK
                     GLITERVLKDVSPSKATSLSIFELNYLLSKTKVKDVMITDIITASPDMLIEDAAVLLR
                     SNVVGSLPVVSEDNQLVGIITRTDIIDAFIEFLGTNDHGTRISLEVKNEIGTFAKIAN
                     IIKEHGINIHHVSNYDIDDNKCEMIIRLNTTDVEALVDSLKNEGYNIFSVVKKE"
     gene            complement(16682..17383)
                     /locus_tag="PLG49_02650"
     CDS             complement(16682..17383)
                     /locus_tag="PLG49_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16245.1"
                     /translation="MLKVKNINVYYDAIHAIKDVSFKVNEGEIVTLIGANGAGKTTIL
                     HTVSGLMKAKSGEISFQKQKIDHLEAHKLLQLGIAHVPEGRRVFAEMTVQENLELGAF
                     TKKDKKAIKRNFEMVYEKFPRLKERRKQIAGTLSGGEQQMLAIGRALMSEPKLLLMDE
                     PSMGLAPILVQEIFNIIKEINAAGTTILLVEQNANMALSIAHRAYVLETGKVVLEGNA
                     KDLLGNDEVRKAYLG"
     gene            complement(17395..18153)
                     /locus_tag="PLG49_02655"
     CDS             complement(17395..18153)
                     /locus_tag="PLG49_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16246.1"
                     /translation="MALLETKDLGIAFGGLRAVGDFNIKIDEKELVGLIGPNGAGKTT
                     VFNLLTGVYLPTEGTIYFNGENIVGKKPFEIVEKGITRTFQNIRLFKDLSVLENVKIA
                     FHHKMKYSIAAGIFRLNAYWKEEKEIEEKAMELLKLFDMQDAAYTTAKNLPYGQQRKL
                     EIARALAADPKLLLLDEPAAGMNPQETKELMETIHFIRDKFKIAILLIEHDMSLVMGI
                     CERIIVIDYGMIIATGTPEEIKRNKKVIGAYLGE"
     gene            complement(18157..19143)
                     /locus_tag="PLG49_02660"
     CDS             complement(18157..19143)
                     /locus_tag="PLG49_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOQ16247.1"
                     /translation="MNSRLKANIINTTSIILIYIILVLLIRIGFINNYYEGILIEMGI
                     NIILAVSLNLATGYLGQLTLGHAGFMAIGAYTAAILSARMNLPDLPLLLVSIVLGGLL
                     AAFFAFLMAIPVFRLRGDYLAILTLGFGEIIRVIILNMKITGGGKGLSGILPLTSFST
                     VYFITVITVTIFYTLINSRHGRAIISIREDEIAAEAVGISTKYYKTFAFTLAAFFAGV
                     AGGLFAHHTLVLDPKDFNFIRSIEILIIVVLGGMGNLTGSVLAAIIITILPRLLMGFA
                     DWNMIIYSVLLIAMMLFKPGGLLEKREFSLNGLLSGKKKSQGNTPVKNEGSE"
     gene            complement(19153..20040)
                     /locus_tag="PLG49_02665"
     CDS             complement(19153..20040)
                     /locus_tag="PLG49_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOQ16248.1"
                     /translation="MDVLAKFLQQLINGIHVGSIYALIALGYTMVYGIIKLINFAHGD
                     IIMMGAYFTFVCIVTFNLPIGVALITPVILCLLSGILIERVAYKPLRNSPRLSALITA
                     IGVSFLLENVFFKKFGQNPIPFPQIINPSPISLGPLQISRITFITVLISVLLMILLDL
                     FIKKTKPGKAMRAVSEDPGAAQLMGINVNSTISLTFATGSALGAVGGILYSIAYPLID
                     PFMGVMPGLKAFVAAVLGGIGIIPGAMLGGFIMGIAESLTKGYISSQLADAVVFGILI
                     VVLSLKPSGLLGKNVKEKV"
     gene            complement(20091..21242)
                     /locus_tag="PLG49_02670"
     CDS             complement(20091..21242)
                     /locus_tag="PLG49_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ16249.1"
                     /translation="MRRLRKLMSLTLTGVLLAGALAGCGQKDSGSADSDVIKIGGIAP
                     LTGNVAVYGVAASNGAKLAIEEINASGGILGKKIEYIEYDDKGDTTEAVNAYKKLVNN
                     NKVNAIIGAVTSKPTLAITPLAAKEGIPMITPTATALDVTAAGTNIFRTCFIDPYQGE
                     IMAKFALENLNASKAAVLYNTADDYSLGVAEAFKNYFEANGGTVVSYEGYGADEKDYK
                     AILTNVKTQNPDVLFLPDYYNTVGLIAQQVKEVGINATLLGADGWDGVLTVAPEAVEG
                     GYFCNHYSTDDTAEEVQNFIKKYKETYNEDPVSFAALGYDTVKILAAAIEKAGTTDYD
                     AVVKALAETNITSVTGTITYDENRNPVKDVAIIKIENGQNRLYTKVNPQ"
     gene            21488..22594
                     /locus_tag="PLG49_02675"
     CDS             21488..22594
                     /locus_tag="PLG49_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOQ16250.1"
                     /translation="MIKIKKNCQLDAKGAFLANYNFSDLIFFDIESTGFSIEYSNVYL
                     IGAVYYQKNQWVLEQFFAENLSEEKKIIEEFFTLASAYNYIIHFNGDTFDIPYLEKKA
                     SLHHLEAPFKKITSIDILKLIRPYQKILGLENCKLKTIEHFLDINRKDIYSGKELIEL
                     YQQFCKKHDPKVKENILLHNEEDLLYLIPLLKIFDLIQFIKDLKVNILDNIQAFDITV
                     DNDLLFSFALLKAAPLSLQLKNENWACSITKDSLHLDFKVKLVEDTMYHFFPNYKDYY
                     YLIDEDEAIHKSLGAFLPKDKRKQAKVSNCYTKRKGLFLQTFQPDIRLPVFKKSYDSK
                     EMYILLDDNKIDHQKLNLKDLALAFIDSQIPRSL"
     gene            complement(22624..24270)
                     /locus_tag="PLG49_02680"
     CDS             complement(22624..24270)
                     /locus_tag="PLG49_02680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ16251.1"
                     /translation="MKLTFLGAAKTVTGSCFLLEANNKHILIDCGMFQGADQEENLNY
                     EPWPFDVTKIDYLLLTHAHIDHSGKIPKLCKDGFKGKIYCSRATADLCAIMLPDSGYI
                     QETEAEWKNRKRLRAGKPLIEPLYTYEDAVKCMKFFEPVAYHEVVNLRGNITIKFSDA
                     GHMLGSSILEIWVNENNEETKLVFTGDLGNYDIPILKDPERIYSADYLIMESTYGNRI
                     HKERENKARKLLNIIEETINKGGNVIIPSFAVGRTQEIIYELFKYNEVDEADVNFLNT
                     VPVYVDSPLAISATEVFRKNLDCYDDEARYYIKNGNNPLDFPSLTFTKTPEESKALNE
                     DKEPKIIISASGMCEAGRIKHHLKHNLWREECTILFVGYQAPGTLGRRILDGAKKVKI
                     LGEEISVNARIENIEGYSGHADQAGLLNWLQQFTKMPKKVFIVHGEPEAQATLAKLIK
                     KQFNVDVAIPERGDNYELTLHKAIPQKPYGALKENFLRLQLLAQVDALSEELYFLTKS
                     LNKELLNNKEDDELMAIKAKIEDVYRAIEQINQSINKPAV"
     gene            complement(24338..24877)
                     /gene="pgsA"
                     /locus_tag="PLG49_02685"
     CDS             complement(24338..24877)
                     /gene="pgsA"
                     /locus_tag="PLG49_02685"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOQ16252.1"
                     /translation="MNLPNKLTILRVIMIPLFLLILLTNWLPDPQKRIIAVIIFALAS
                     FTDYLDGYIARKNNLVTNFGKFMDPLADKLLVTSALISLVELGDLSAWIAILIISREF
                     IITGFRLIAASDGIVIAASWWGKIKTVSQMLMIIIVLLNINTTLFNYLEYLLMGAVIL
                     FTIISCIDYIAKNIKVLKE"
     gene            complement(24861..26204)
                     /gene="rimO"
                     /locus_tag="PLG49_02690"
     CDS             complement(24861..26204)
                     /gene="rimO"
                     /locus_tag="PLG49_02690"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOQ16253.1"
                     /translation="MTLKISFISLGCDKNLVDSEVMLYLLNQAGFSLISDESQADVIV
                     INTCCFIQDAKEESIENILEAAEYKKDGNCKAIIVTGCLAERYKDEILSEMPKVDAIV
                     GTASYREIVEVIKEVMAGKKVEKFSDINISLSDDSERILSTAGYYAYLKIAEGCDNAC
                     TYCVIPKIRGKYRSRSMESLIKEAKNLVNQGVQELIIVAQDTTRYGVDLYGEKRLPIL
                     LKELCKIEDLKWIRLLYTYPEEITDDLIEVMATEEKICNYLDMPIQHSNNAVLKRMGR
                     KSTKEALIQLINKLRDKIPDICLRTTLITGFPGETEEEFEDMKEFVKTMKFDRLGVFS
                     YSKEEGTPASRLKDQIPKKIKEQRKNILMALQRQICAEKSKEFIGKHLEVLIDGKIAG
                     EDVYCGRTYRDAPDIDGMVFVKTKGELLSGEFVKVKITEAEEYDLVGEIIDEFTE"
     gene            complement(26224..27069)
                     /gene="folD"
                     /locus_tag="PLG49_02695"
     CDS             complement(26224..27069)
                     /gene="folD"
                     /locus_tag="PLG49_02695"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOQ16254.1"
                     /translation="METLIIDGKKISQDIKDELKAEVEDLKKKGIEPGLAVILVGNNP
                     ASKVYVSNKKKACEYIGIRSFSYELPEETSEEELLKLIKDLNQTKEVHGILVQLPLPK
                     HIDENKILLSIDPSKDVDCFHPYNVGMISIGELNGFLPCTPAGIIELIKRSQIEIEGK
                     KCVVVGRSNIVGKPVANLLLRNNGTVTICHSRTKNLKEICKEADILVAAIGKANFITA
                     DMVKDGAVLIDVGINRLENGKLCGDIDFENCKYKAMAITPVPGGVGPMTIAMLMKNCV
                     KAAIM"
     gene            complement(27081..28751)
                     /locus_tag="PLG49_02700"
     CDS             complement(27081..28751)
                     /locus_tag="PLG49_02700"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOQ16255.1"
                     /translation="MKTDIEIALEAQMKPITEIAAQLDIEPDDLELYGKYKAKISESL
                     FEKVKNNPDGKLVLVTAINPTPAGEGKTTTSVGLGQALCKMGKKAVIALREPSLGPCM
                     GVKGGAAGGGYSQVVPMEDINLHFTGDIHAISTAHNLLSAMIDNHIQQGNELQIDPRQ
                     ILWKRVVDLNDRALRNIVVGLGGKAHGMPREDGFMITVASEVMAILCLAKDLEDLKRR
                     LGEIIVAYNFNGEPVRAKDLKANGAMTALLKDAIKPNLVQTLENTPVIIHGGPFANIA
                     HGCNSIRATKLALKLADIVVTEAGFGADLGAEKFFDIKCRFAGLKPDAVVLVATIRAL
                     KYNGGIPKNQLSIENLDALKKGFANLRKHIENIKLYGVPVVVTLNAFLTDTQAEIDYV
                     KEECEKAGCAFAISEVWEKGGAGGIELAQKVIDTLENKKSEFKVLYDHNAPVKEKIES
                     IAQKIYGASKVNYTSKALKDLENIERNQFDKMPICIAKTQYSLSDDPALLGCPKDFEI
                     TVRELRVSAGAGFIVALTGDIMTMPGLPKEPAAIKIDVNEKGQITGLF"
     gene            complement(28778..31111)
                     /locus_tag="PLG49_02705"
     CDS             complement(28778..31111)
                     /locus_tag="PLG49_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOQ16256.1"
                     /translation="MGKRMTNRKKNNQFKKTGSPLSQEIKGIIIVTIALILAVGIYTK
                     QGGIIGEWIKQLSLGLLGIGAYFLPVVFTVIGILILFKKQGKITKSKLFAASAIIILL
                     STFLHILYLEPDMLITSKDMSLWSFMTYYFHIGNWNNGGLLGALLANLLLKLVGRYGG
                     YIIIISLFIVLFILLTDNSFIEKFKMIIKGISQFFKKLFHLKSGKKKDKIKNQNKSQE
                     KDYIEKTDDLITVLDFTEKKDDEETQLEITANLTEEDYSEINRAKEKENKKNNNNNEI
                     LSIEKSISTKKEPKEPVDYHFPSIDLLQKNPYSPTTDSKNKMLDNAKKLEKTLYSFGV
                     EAKVVQISRGPTVTRYELQPRQGVKVSKIVSLADDIALNLAASGIRIEAPIPGKAAVG
                     IEVPNQKAQAVYLREVIEDEEFQKFPSKLAFALGKDIAGNPVVADIAKMPHLLIAGAT
                     GSGKSVCINTLITSILYKARPDEVKLILIDPKVVELSVYNGIPHLMIPVVTDPKKAAG
                     ALNWAVQEMTNRYKLFAEYNVRDIKGYNHLISAKEESNLEILPQMVIIIDELADLMMT
                     APNEVEDAICRLAQMARAAGIHLVIATQRPSVDVITGLIKANIPSRLAFAVSSGTDSR
                     TILDMTGAEKLLGKGDMLFYPVGANKPVRIQGAFISDSEVEALVNSIKQVKEAEYDQD
                     IIENINDINNFTPDEDLDEYLEKAIEIVVEKEKASISMLQRYLRIGFNRAARLMEEME
                     ERGVVGPEEGSKPRKVLISQEEFRQMKLKSQQNSNNN"
     gene            complement(31226..31441)
                     /locus_tag="PLG49_02710"
     CDS             complement(31226..31441)
                     /locus_tag="PLG49_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HOQ16257.1"
                     /translation="MIYSVISQDIIFADSERDRNIQFEEIYYKGSLLQVYYNENKNYI
                     IERILSTNPYDYLNDELQPGNTIKISY"
     gene            complement(31438..32112)
                     /locus_tag="PLG49_02715"
     CDS             complement(31438..32112)
                     /locus_tag="PLG49_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOQ16258.1"
                     /translation="MTETIKEFGQMPIEENKEDEKSKIHCLTIIGQIEGHILLPPQNK
                     TTKYEHVIPQLVAIRDNEDIKGLLVILNTVGGDVEAGLAIAEMIATLGKPSVSLVLGG
                     GHSIGVPLAVAANYSFIASTATMTIHPVRMSGTVIGIPQTFDYFEKMQERIVEFVARN
                     SGISQKRFKELMLDTGMLAKDVGTILVGEEAVKEKLIDEVGGLDKAIKKLYQLIDENN
                     DKEEAK"
     gene            complement(32284..32871)
                     /locus_tag="PLG49_02720"
     CDS             complement(32284..32871)
                     /locus_tag="PLG49_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HOQ16259.1"
                     /translation="MNRLKGIKIGFALCGSYCTYDKVIPAIQTLIDEGAEIFPIMSNN
                     ASSTDTRFGKAEEHKEKIEKMTGKKIITSIVEAEPLGPNNLIDILVIAPCTGNTLAKL
                     ANAITDTPVLMAAKALLRNEKPVVLAVATNDGLGLNMKNIGILMNSKNIFFVPMGQDN
                     SEKKPNSIMSDMNLILNTVEQALQKKQVQPVIIKL"
     gene            complement(32868..33752)
                     /gene="dpsA"
                     /locus_tag="PLG49_02725"
     CDS             complement(32868..33752)
                     /gene="dpsA"
                     /locus_tag="PLG49_02725"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HOQ16260.1"
                     /translation="MTYHRIRFAVIGGDLRQIKLINLLASKGYQVNMFGFDNKNIDSG
                     VNLFNNIRELISDADIIVGPIPCSRDNKTVYSDYLDSSIMLEDIFKNISEDKIFMAGS
                     ITPQIKEIAEQYPFKVIDLLEREELAILNAIPTAEGAILYAMGNSEITIHGSKCLVLG
                     FGKCGKVLAHKLKGLDADLTVEARKAEDLALIKSYGYKALYLQDLEKFIGDYDFIFNT
                     IPALILNRDLLQKIKKSALIIDIASKPGGVDYSAAKELGIQAILAPSLPGKVAPESAA
                     LIIYETILNVIRDMGVII"
     gene            complement(33879..34331)
                     /gene="dut"
                     /locus_tag="PLG49_02730"
     CDS             complement(33879..34331)
                     /gene="dut"
                     /locus_tag="PLG49_02730"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOQ16261.1"
                     /translation="MSDRLKVFIKKVENAQDLPLPTYMTEQSAGMDLYANVEEDVVLK
                     KGDIKLIPTGIAIQLPKGYEAQIRPRSGLAYKYGIGILNSPGTIDADYRGEIKIIMIN
                     FGSEDFIIKRGERIAQMVINKIESVEWVQVEELDRSERGSGGFGHTGR"
     gene            complement(34316..35584)
                     /locus_tag="PLG49_02735"
     CDS             complement(34316..35584)
                     /locus_tag="PLG49_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOQ16262.1"
                     /translation="MFKKKKLANGVTIVAEKIPSVRSIAFGVWVGNGSRHEKKENNGI
                     SHFIEHMMFKGTSNRTAKQIADEMDRIGGQLNAYTTKEYTCYYFRALDNHFSQALDIL
                     SDMFFNSTFDNREIEKEKQVVLEEINMYEDSPEELVHDLSQTAVWEESSLGQPILGSS
                     KSISRFDSERLFSYYHKRYRPENTVIALAGNFNYNEIFAEIEEKFASWQAKEKDDYVY
                     KIPNYKPQLITKLKDIEQVHVCINFPGIHSGSEKIYDLITLNTIFGGGMSSRLFQKIR
                     EERGLAYSIYSYPTNYKDTGLFTIYGGMNPSQTIEVIKLIMEEIHLLKKEKISHEDLN
                     KTKEQLKSNYILGLESTGSRMSSIGKSQLILNKIKTPDEIIESVDAVTQDKITELIEQ
                     IFDFSKISISIVGKIDHNKLKEIKELCLID"
     gene            complement(35631..37742)
                     /locus_tag="PLG49_02740"
     CDS             complement(35631..37742)
                     /locus_tag="PLG49_02740"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOQ16263.1"
                     /translation="MSKIYSVDLAGRKLTVEIGKVAELANGAALIRYGDTVVLVTATA
                     SDKPREGVDFFPLSVDYEERLYAVGKIPGGFIRREGKPTEKAILTARVIDRPIRPLFP
                     SDYRNDVSVVATVLSVDQDCSPEIAAMIGSSVALSISDIPFYGPTGSVNVGYVNNKFV
                     INPTAEQREISRLSLTVSSTKDRVMMIEAAADEIEEDLMVEAIFLAHEENQKIVKFIE
                     EIQAECGKPKHDYEKHEVPCELYEEIKEFIGTEEMEKAVFTDDKQQRDIQIKELTEKV
                     TAHFLEKNPDYEPYIVEAISKFEKETVRNMILRKHKRPDGRALEEIRPLSSEIDILPR
                     THGSALFKRGQTQVLTVTTLGALGDVQILDGLDALEDTKRYMHHYNFPSFSVGETKPS
                     RGPGRREIGHGALAEKALLPVIPSEEEFPYAIRLVSEVLSSNGSTSQASVCGSTLSLM
                     AAGVPIKAPVAGISVGLVTGESLDDFVLLTDIQGLEDFFGDMDFKVAGTHKGITAIQM
                     DIKIRGLNREIIEKALYQTKRARTYILDEVILKTIPEPRKTLSPYAPLILQTTVDPEK
                     ISEIIGPRGKTINKIIDETGVKIDIEDDGRIYVCGVDKEKTKRAVSIIESIAKDIQPG
                     EIYLGKVVKTTNFGAFVEIAPGKEGLVHISKLAHEHIKNVEDVVKVGDEILVKVTDID
                     SQGRINLSRKDTLAKSEEKEE"
     gene            complement(37965..38228)
                     /gene="rpsO"
                     /locus_tag="PLG49_02745"
     CDS             complement(37965..38228)
                     /gene="rpsO"
                     /locus_tag="PLG49_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ16264.1"
                     /translation="MDKETKREIIKKFGRNENDTGSPEVQIALLTYRINHLTEHLKTH
                     KKDHHSRRGLLKMVGQRRGLLNYLMKKDIDKYRELISELGIRK"
BASE COUNT        11889 a         7184 c         5822 g        13503 t
ORIGIN      
        1 tgggtcaatt tctgtccggc gctatgggtc aattataccc cggcggtgac aatataatct
       61 tttatcttat atacagttat ttattctata ttattattta ttcattaaaa tatttgctgt
      121 ctatttataa attgttttat cctatatttt tattaataat tttatcaatg ctttaattta
      181 tatatatatc ttaataaaat acgtaaaatt tttaaatgta ttttcatttt tattacttat
      241 taatgggtta ttttagattt aagtaatggt ttttaaattt aaattgttta ataatcaatg
      301 gcgctaagct ttatttataa actgaatttt ataatatacc caatttattc ataataattt
      361 acaaatattt acatttaaat cataagtagt gtagccctgt tttcagacgg ccgtatataa
      421 ataattccct attgctatta taaataactt atttatcgta ttaacaatct ttatataaaa
      481 aaggctaata ctttttcttg attccaataa aatctataga tataatatag attgcaatta
      541 ttttatttat acttttatca tctatctata ggaaggaagt tcagatgaaa ttacaaagat
      601 tacttagtta tgttcgccgt gctgttgatg attataatat gattgaagat ggagataaaa
      661 ttgccatagg aatatctgca ggtaaagaca gtttaatcct gcttttagct cttcggtcat
      721 tgcagcgatt ttatcctaaa aaatttgaat tagaagccgt tacaatttct ttaggctttg
      781 ataattttga tcttactggt gttcaaaaaa tgtgtgatga aattggtgtc cgttatactg
      841 taaaagaaac cgacatagct cagattgtat ttcacgaacg taaagaaaaa aatccttgtt
      901 ctctttgtgc caaaatgaga aaaggtgccc tgaatgatgt agcaaaatcc ctgggctgta
      961 acaaagtagc tttgggtcat aacaaggatg atgttattca aacccttttt ctttccttgt
     1021 tttatgaagg tcgtatatat accttccctc cagttagtta tttagacagg tcggatttgc
     1081 atacaatcag acctttaatt tacgttccgg aaaaagaaat cgtgggtttt gcccgaaagg
     1141 aaggtctccc tgttttaaaa agcccctgtc cggctgatgg taataccaaa agagaacagg
     1201 tcaaaaattt gattgcagat cttcgaaatc aatacgataa tctggacaat cacatttttg
     1261 gagccatcaa gagatccgct atcaatggct ggaatttgga aaaacacctc taaagaggtg
     1321 ttttttctcc tccgcctgca taatctttat atacatcgat ataaatttct ttttgctgat
     1381 taacggctgc atacatcact tcttttatat cttgaatccc tttagctttt aattgcataa
     1441 acagccagtc ttcatcaatt ttcattaatc tcatattatc tttaatgatt ttcccatcta
     1501 aaataatgct gcaaaaaggt gccttgggac ttgtaacaag acctaaatct tgtaccgtag
     1561 gtacttctaa atgttcatat ttcatcacac ttaaagcccc attggtttct acaatggcat
     1621 attttacttc tttaatatca aatatttttt caagtctgag ctgctcaagt aaatcattga
     1681 tactataaag gcatcttttt aagttctttt ctaatatttt cccatcttca atgacaataa
     1741 ccggttctcc ctgtaacaat tgattaatgc catcattttt taaagaaata taagacatac
     1801 cccaggttaa tattccaaat aaaaataagc caattaaatg atgccaggtc cgctgattta
     1861 aatccgtggc taaagttgct gcaattgaac caaaagtaat accattaata tattcataaa
     1921 cggtcagttg ggcaatttgt tttctgccga gaattctggt aataaaccat atactaaaaa
     1981 aagctaaaag ggtttgtatg cctatttcac aaattacttt caagtgatca cctcctgaca
     2041 taaaaatacg gattctacta ataatataag ttaaataaac agaatttatc ggaggtaata
     2101 attatgaaat tatggatgca ggccttattc actctaattt ttattattgc ttgttttatc
     2161 attatttatt atttgcacag aaaaaagaaa atttaaatgc tcagctgtct gaaaaatatc
     2221 gtcagaatta aataaatatt tgccatacag aacatatatt tgtataataa atatatataa
     2281 tgtaaaaatg gatggtgttc atatggcaga aattaattat catgaacaag aaaaacaaaa
     2341 aaataccctt cgtttaagga ctttattaaa gtctcttccg ccctttgcct ttgaattttt
     2401 cagaggaatt gagccaacga cttctcccag gaccagaatt gcctacgcct atgatttaag
     2461 gatttttttt gaatatattt tagaaaacca cccaaaactt aaaaataaag taatcaatga
     2521 attagaaata aaagatttag aagaaattac acctgaccat attgaaatgt ttttagaata
     2581 tctcaattat tatagcaggc ccgatcccaa taatcctgac aaaatcgtgg aataccaaaa
     2641 tgatgaaagg gctaaatcca gaaaattagc tgccatcagg accttttatt tgtattttta
     2701 taaaagaaga aagatttcaa cgaacccacc ttctcttgtc gaaagtccta aaatccatga
     2761 gaaaaatatt atcaggctgg aagtggatga agttgccaga ctattagacg aagtggaatc
     2821 aggtgaaaac ttaaccgata cccagaaaaa ataccatcat tatacaagac tcagggactt
     2881 agccttaata acccttttac tgggaacagg tatccgggta tcggaatgtg ttggattaaa
     2941 cattagtgat attgattttg atgtgaatgg tttaaaaatt atccgtaagg gcggaaatga
     3001 aatggttatt tactttggag atgaggtcga agaagccctt cgaaattatt tggaagaaag
     3061 attaaaaaaa cagcctaaac ccggtcatga agatgctctt ttcttgtctt tgcaaaaccg
     3121 gcgcctgact gttcgttctg ttcaaaattt agttaaaaaa tatgcatcct tagtgactaa
     3181 gttaaaaaat atatcccctc ataaattaag aagtacatat ggaaccaact tatatcggga
     3241 aaccggagat atatatctgg tagcagatgt attgggccac aaggatgtca atacaaccag
     3301 aagacattat gctcaaattg atgatgacag gcggagaaag gcagctaaag tggtcaagtt
     3361 aagaaaaatt taaaccggct tttagtaaat gggtataata attgtatctc cagcatataa
     3421 tctgtcgctt tccaggcgat taaattcctt gataagttta acgtattctt tagtactttt
     3481 atcttcaggt ttatattttt gagcaagttt ccaaagggag tctcctctat gtatcatgac
     3541 ttcttcgtat tttttaacca aatgattgtt aaaggattca tgacctgtcg aacagtaagt
     3601 agataaaatc gaaaacaatt gtatcaaact aatagcaaat gcaaggagta aaaataaacg
     3661 gaatcttttt ttcttttgca tattcatcct cctgacataa aaacttttat cgttatgttc
     3721 tgttgttcag aatatatgtt cgattatcat tatatacgaa catatattct gagtcaatga
     3781 tttttttgaa attatttgat aagtttttat ggttagagat atttccttat aaaattaacc
     3841 gtttatcatc gatattttta aagaaaatcg aacataagtt tgatatttcg aaagacttat
     3901 ggtataataa gtcatattat atagtgaggt gactatatga gtaataaagt tggaggtaag
     3961 caactggaaa tactaaatta tttaaaaaaa gaaataaagg aaaagggata ccctccatcc
     4021 gtcagggaaa tatgtgatgc ggtggattta aaatcaactt caacggttca tggccattta
     4081 aaccgtcttg aacagaaagg tttaataaga agggatccaa caaaacccag ggccatagaa
     4141 attttagatg agtcttttta tccgatgcga caagagttgg ttaatgttcc catcatagga
     4201 aaggttactg ctgggcaacc tattcttgct gttgagaaca tagaagatta ctttcctctt
     4261 ccagtggatt acgtaaaaaa cgatacggta tatatgttgc atgtacaggg cgatagtatg
     4321 atagaagccg gtatttttga taaagatttg attttagtcc gcaaacaaag tactgcagaa
     4381 aatggtgata ttgtggttgc tttaatagat gaagaagcaa ccgttaagag atttttcaga
     4441 gaaaaagatc atatcaggct gcaacctgaa aatcctgcga tgagcccgat tgttgttagg
     4501 gatgttacaa ttttaggaaa agtgattggt ttgtttagaa aattttaatg tttctaggag
     4561 ttgatcatgt ggaaaatggc tttattcgag taggagctgc tgtacctaaa ataaagcttg
     4621 cagattgtaa aagtaattca gaagaaatca tccgtttaat tgaagaggcg caaaataatc
     4681 ttgttcaagt tttagttttt cctgaattat ctcttacagg ctatagctgt ggtgacttgt
     4741 tttatcagga tattctcctt gaagaagccc aaaatgggct tcaaaaaatt cttgattata
     4801 cagctgataa agatatttta attcttgttg gtatgcctct ttcagtggat aatcagcttt
     4861 ttaactgtgc tgtgcttttg ttaaaaggta acattttagg tgttgtagca aaaacatcca
     4921 tccctaatca taatgaattt tatgaaaaaa gatggttttc atcaggaaat catgctgtaa
     4981 gtaaagaagt caatttatgt gggcaaaaag tgccctttgg caatgatctt ttattccagt
     5041 gcagtaatat taatcattta tgtattggag tggaaatctg tcaggattta tggacaacgg
     5101 ttccaccaag ttctttgcaa tccatgcaag gtgctaccct tattttaaat ccctcggcaa
     5161 gcgacgagta cgtgggaaaa aggctttata gaaatcattt ggtattacaa cagtctgcta
     5221 aatgtatggt gggctatatt tatgcttcct gtggctatgg agaatcaact tctgatttgg
     5281 tttacagtgg gcattctatg attagtgaaa atgggactct tttagaagaa gcccttccct
     5341 tttcccatga cagttatttg atatttactg aattagacct tttaagaatt gtttcagaac
     5401 gtaaaagggc tcctgttagt ggagatctca tgggtaagaa tatcaatcag tttatttcat
     5461 ttagaaaagt ctattttgaa ttaaatcaaa ataaaattcc aactttatca cgaaagattg
     5521 agcctcatcc tttcgttccc caagaccctc agacaaggga attttattgc agagaaattt
     5581 ttgcaattca aaccaatggt ttaaaaaagc gtgtagagca tacaggggca gataaactta
     5641 tattaggtat ttctggcggt ttagactcta cccttgccct tttggtatgt gtagaaactc
     5701 ttgattgcct aaaaagggac aggaaagata ttatcggtat taccatgcct ggttttggaa
     5761 caagtggacg aacctatgaa aatgccattc aattaatgaa aaatttaggc atttcaatgc
     5821 gggaaatatc tataaaagag gcttgtattc agcattttaa agatattgat catgatgaaa
     5881 agctttatga ttccacctat gaaaatgcgc aggcaagaga aagaactcaa atattaatgg
     5941 atattgccaa taaagaaaat ggtttagtta ttggtacagg tgacttatct gaattggctt
     6001 taggctgggc aacttataac ggagaccata tgtcgatgta tgctgtaaat actggcgttc
     6061 ccaaaacctt attaagtccg ctgatagcat ggatttcaaa tatgcctaaa tatcataata
     6121 taaaaaccat tactgaagat attattcata cacctgttag tcctgaactg cttcctccag
     6181 atcagaacgg agagattaat caaaaaacag aaaacatttt gggtccttac gaacttcatg
     6241 atttcttttt atactatatg ttgcactttg gctttaggcc ttcgcggata ttatatcttg
     6301 cagaaacggc ctttaaagat aaatacagcc gggaaagcat tattcaatgt ttaaaaatat
     6361 tttataaaag attttttatt catcagttta aacgctcttg ccttcccgat ggtcctaaag
     6421 tatgttctgt atccctatcc cccagagggg atttccgaat gcccagtgat gcttcttaca
     6481 ggctctggct ggatgaaatt gaattactgg aataaaaaag agaatggttt taccattctc
     6541 ttttttaatt cttaaacatg aattaatttt ttatccatca tattttgtaa agcaatcatg
     6601 atgcctaact ttgcatgatg ccaggaaaga ccaccttgta aaaatacagt atatggttct
     6661 ttcatcggtg cgtcagcact aagttctata gaagagccct gtacaaaagc acctgctgcc
     6721 ataatgacct ggctatcata acctggcata tcagaaggtt ctggtataac aaagctgtca
     6781 acaggagagc ctttttgtat gccctgacaa aaagcaatga cattttcagg ctttttcatt
     6841 tgaatggctt gaataatatc atttcttttt tcgcatgaag caggacttac ggtaaagtcc
     6901 aatttttcaa aaattcttgc ggcaaaaatg gcccctttca aacttccaga taccacttgt
     6961 ggtgcaaaga acaagccctg gagtatgggt tgatttaaac ctaaacttgc cccaacttct
     7021 ttgcccagac ctggagcagt taaacggatg gcagcatttt caacatattc ttctttacca
     7081 acaatatatc ctccaatagg agcaatacca ccgccgggat tttttattaa agaaccgacc
     7141 actaaatctg caccaacttc agaaggttct atataatcaa caaattctcc ataacagtta
     7201 tccaccatgc aaatgacatc attcttaatc tttttaataa aagcaatcag ctcttttatt
     7261 tgctggacag ataaagtagg ccgccataaa tatccttttg agcgttggat cgttaccatt
     7321 ttggttttgg gcgtaatggc attttcgatt cccttataat caaagccgtc ctctaataaa
     7381 tccacttgct tgtatgtaac accatattcc ttaagggaac ctttcatttt tcgaattcct
     7441 attacccctt caagagtatc atagggttta cctataggag ataataactc atctccaggg
     7501 cgcagatttc caaataaggc cactgttaaa gcatgggttc cagaaactaa ttgaggacga
     7561 accagtccag actctgcttt aaaaacttct gcatatatgt tttctaccgt atccctgcca
     7621 aggtcgttat atccatagcc atttgttgct gcaaaatgta tatcacttag atgatttttt
     7681 tgcattgcag caagaatttt tagctggtta tattcagcta tatgatccac ttcgttaaaa
     7741 tagggttcta tttccttttc aattttttta caaaattgaa tgacttcatt gcttaagcca
     7801 aagtttctgc gtaaaatttc gtcaacgaat tcgtgcattg aactatctcc attctactcc
     7861 ccttcatttt tatcatttac gttataatat acaggttttg ctggaatgat ggttgaaatg
     7921 gcatgtttat aaatcatttg ctgtttgccg tctgactcta acattacaat aaagttatcg
     7981 aaacctttta caactccttt taattgaaaa ccattggtta aaaatacggt tacagtcatt
     8041 ttttctttac gtacctggtt aagaaataca tcttgaaggt taatagtttt gctcacaaaa
     8101 atccactcct cttttgatat taggttatat atatttattc tatatgataa tgccaatttc
     8161 ttcaatataa tttaacattt ttgcaagaat aatatccata tcaaaaccaa aatcgtctat
     8221 atttatccaa tatggatttt cctgcctttt gaaccaggtt aattgtctct ttgcaaaatg
     8281 cctggtgtcc ctttttagta tataaatagc ttcctcaaga gaaatatttc cttgcagata
     8341 agggacaatt tccttataac ccagtccctg catagaaaca agatctggag aataaccttt
     8401 tttaagtaaa gcttgaactt cttcaattaa gccagcttca atcattttat cgactctttg
     8461 ctcaatccgt ttatataatg tttcccgttt catgtttaaa caaaaaaaag ctgtattata
     8521 aggagatact tttttatttt tttcttcttc attgtgctgt gaaatgggct tgttggtttg
     8581 tttataatat tccaatgccc ggataactct tttgacatta ttgggatgaa tttttaaggc
     8641 ggaaactggg tcaacttttt ttaggatctc atgaatatat tgatttccct tttctttagc
     8701 caacttttcc agataacttc tatattcata atcgttgttg gtttccataa aattaatatc
     8761 gtaaaccaac gcttgtatat aaaagccagt accgccaact aaaataggta ttttattttt
     8821 attgtaaatc atttctaagt attttttagc tttttcttga aatatagcta cattaaattc
     8881 ttcatcaggc tcaatttcat cgattaaata atgaggaata ttctccattt cctcttttgt
     8941 tggcttggct gtaccgatat ccatatactt atacacttgc atagaatcgg cagagatgat
     9001 ttccgcatca atcttttttg ccaattggat agaaagattt gttttgccac aagcagtcgg
     9061 tccggcaata acaattaaag gttttttcat agttcctcca ttctattgaa cccgtttaaa
     9121 ttttttttcc agttcatatt gactcataga tatgatggtt ggtcttccat ggggacaagt
     9181 aaatggatta tccagattaa gcaattcctc aattaatgct ttggcttcca cagtactgag
     9241 ctgatctttg gctttaacag ctcctttaca ggataaaagg gcaatatgat taagcttcat
     9301 atcataaagg tttttaatcc tttcatcatt taaagtatcg ataatttcta agaaaaaact
     9361 gctgtcaaca ggtccttcaa aaagaatggg cagttcccgg accaggtatg cagattcccc
     9421 aaattcttca atttcaaaac caaatttttt aaataaagaa atgttatctt ctacaatctc
     9481 tttttcctta ggagtgacct caaaaacaac aggctctaat aatggttggg aataaacttt
     9541 accttgctta aaatcatgaa taaatttttc ataaagaatt cgctcatggg ctgcatgctg
     9601 gtcaacaata taaagactat tgtcttgttc aacaagccaa taagtctgaa aaacttgccc
     9661 tataatttta taatgacgaa agcttggagc cttgtcttct ttatgaactt tttctttttc
     9721 aaaaatggta caggcttctt ttttttcagt accacatggt ttattttcat aaagattttc
     9781 tttatgagaa aagttaagct cctgattaga ttcttttact tttatatatt tatcctcagg
     9841 actgaatacc ggatggatgc ttatatcttt atactccaag ttttttcttt ttatttcaaa
     9901 aggttctggc aatcttccct gaacgggttc gggattaaaa agtttttctt ttttattgtc
     9961 taaattaacc tgaggaatta aatcttcttt aaataaattt ttatgaattc cattatagat
    10021 aaactgatag atttcttctt catccttaaa gcggacttcc atttttgtcg gatggacatt
    10081 aacatcaatt ttattcgctg gaataaaaat atgcaaaacg acaacaggaa acttattaat
    10141 tggcagcaaa gtgttatatg catcctctac tgcattttct aatattttac ttttaatata
    10201 ccttccattg atatagaaat tttcataagt cctgttggcc cggtttaagg aaggcttgcc
    10261 aattaagcca atgagcttta gatcctgttc tgtttcttca atagaaagca tatgctttac
    10321 aacttctttt ccataaacat taaatacggc attttttaac tcgttgtttc cggaagtgtg
    10381 aaaaacaact gaattattat taataaattt aaaggagacc tccggatgtc ccagggcaat
    10441 tttatagacc atatcactga tataagagct ttctgtagca ggctttttta aaaatttttt
    10501 ccttgctggt atattgtaaa aaaggttccg cataataatg gttgttcctt ctggacagcc
    10561 gacttcttct tcagtaatga ttttccctcc atagcattct actctttttc cagttacttc
    10621 tccataggct tttgtaataa tttccaattg agacacagca gcaatactgg ctaaagcttc
    10681 tcctctaaaa cctaaggatt ctattttatc caaatcttca acggcagtaa ttttgctggt
    10741 ggtatgcctg agcaaggctg tcgtaacatc ttccttagga ataccgattc cattatcggt
    10801 tacccggatg agggaaatgc caccatcttt aatttctacc gttatggcac tggcctgggc
    10861 gtctatggag ttttctacga gctcttttac aaccgatgca ggtctttcaa ccacttctcc
    10921 tgctgcaatt ttattaatgg tataatcgtc taatctacgg attggatcca aaaaatcacc
    10981 tcatcatagg agagattttg cttttttgtt taagtctaag agaatatcaa tagcttgacc
    11041 aacagaaatt tctgaaagat ttaaattggc aatttcctta atcaactctt taaattcatt
    11101 ttgctccatg gagaataaat ccatttgaat gacttgaatt tcattaaaaa tattttttgc
    11161 atttttcgta atatctgctt gggtcagttg tgacaagata accttagccc gttcaataac
    11221 ctcattgggc agaccagcca aattagccac ctgaataccg taactgtgat tggcccctcc
    11281 ccgtttaatt tttcttaaga aaataatgtt atcgcctttt tctaaaacat cgatgcaata
    11341 attctttacg ccactcagtt taccttccag ctctgtcagt tcgtgatagt gggttgcaaa
    11401 gagcgtttta gcaccaattt tttctttgtc actgatatat tcaatgactg cccatgcaat
    11461 acttaaaccg tcaaaggtac tggttccccg tccaatttca tccagtatca ataaactttt
    11521 tgatgttgca ttattgagaa tattagccac ttcagtcatt tcaaccataa atgtactttg
    11581 accagaagct aaatcatctg aagccccaac ccgggtaaaa atcctgtcta caacaccaat
    11641 tctggctgtt tttgccggaa caaaactgcc aatttgagcc ataagcacaa tcaaagccac
    11701 ttgtctcata taggttgatt tcccggccat attaggtcct gtcacaatag caagccggtt
    11761 atctttttca tctaaaaaag tatcattggg aataaactgt tctaagggca tcattttttc
    11821 tactaccgga tgcctgccgt cttcaataaa aattgaatcc ccttgattaa cttcaggctt
    11881 acagtaattt tgcttttctg ctacttcagc taatgattgt aaaacatcca caatggcaat
    11941 taatcttgcc gttttttgaa tgcgttctac ttctttttca attctttctc ttatttgaac
    12001 gaaaatatca tattctaatt cgattaattt ttcctctgca cctaaaacgg tttcttctaa
    12061 ttcttttaat tcagccgtaa tatatctttc tgcattggtc aaggtttgct ttcttatgta
    12121 ccggtctggt acagaatcaa tattcgactt tgttacttct ataaaataac caaaaacttt
    12181 attaaactta attcttaaat ttttaatacc agtcttttct ctttcttcgt tttctaaagc
    12241 tgcaatccac tgtttgcctt cagtttttgc ccttcttagt ttatctattt cggaatgata
    12301 acctgtttta ataatgccgc cttctctaac agatatgggc gcttcatcaa caatggactg
    12361 ttgaattaat tcataaatgt cttctaaact gtccattgaa tcataaattt ccttttgata
    12421 agctgcctga cattcgttaa gtaaactttt aatatgagga atcatggaaa tagaattttt
    12481 aagcgcaatt aaatcccgtc cattaactgt tccatatatc accttagtca tcagacgttc
    12541 caagtcatag atgccattta acagttcttt taattcttct cttaaaatag gattgttttt
    12601 aaactcctct actccatcca ggcgcttatg aatttcatta acatccaata agggttgttc
    12661 cagccatttt ctaagaagtc ttgcccccat agctgttttg gttttatcta aaacccaaag
    12721 cagagaccct tttcttgatt tttcccttaa ggtttctgtt aattccaaat ttcttcttga
    12781 agaaagatct agtatcataa attcttcaat ataatacgga gtaatttctg tcacatgact
    12841 taaattaact ttttgagttt cccttaaata ctgaagcaag gccccgcagg cacagatacc
    12901 taaacggaat tcttttaaac caagtccgtc aaggctgtgg actttaaaat gctcagataa
    12961 aattttatac gaactgtcat aatcaaaagc ccaatcccca tattcattga taaaagtatg
    13021 aaacctgttt tttatttctt caattaaatc cgcagacttg ttgaattcac tgttagataa
    13081 aagttctgcc ggattatatt ttgcaatttc atctaacagt ttcttttgat tagaaacttc
    13141 tttaatttct gtgacttgga attcacccgt tgtgatatct acaaccccca ggccatagcc
    13201 atccttagag gcaaaaatac ttaagagata gttgtttttc ccttcatcta gaacattact
    13261 gctaatttgg gttccagggg taataatcct ggtgatatct ctttttacaa taccttttgc
    13321 tgtcctggga tcttcaatct gctcacagat agcaacttta taaccctttt caattaattt
    13381 attaatatag ttttcagcag aatgataagg aacaccacac atgggtgccc tttcatccag
    13441 accacaatct ctgccggtaa gggttatttc taattcttta gatgctgtta aagcatcttc
    13501 gaagaacatt tcataaaaat ccccaagacg gaaaaagagg aggcagtcac tgactttctt
    13561 tttaatttct aaatattgct gcatcatggg tgtaagttgc aaaatgattc cctccgcctc
    13621 attaaaatca atcatctaat tggctaattt accaattaag tagtaagttt ttgcttcgat
    13681 aatttctaca ttgacaaatt gaccaattaa ggatgtatcg ccttcaaaat gtaccagatg
    13741 gcctgaatct gtccggcctg tcaggtaaga atctttgttc ttactttttt cttcaaccaa
    13801 tatttccact gtctttccat aaaattcctg gctcttttca taaacaatcg gttgtaatac
    13861 ctcttgcaat tcattaaatc gtctttggca tatttcttcg ggaacttcac cttccatcgt
    13921 tgctgccggt gttcctgtcc gtttagaata aataaaagta taggccccac tgaatcgtac
    13981 tttcctgaca acatccagtg tatcctggaa atcttcttca ttttctcccg ggaaacctac
    14041 aataatatct gttgttaaag caatgtcagg aatcgtattc tgaatctttt cagccaattc
    14101 caaatattgt tctttggtat atttccggtt cattttttta agaatttctg tgcttccggc
    14161 ctggaaagga agatgaagtg atttacatac tttatcacaa tctctcatgg ccagaattaa
    14221 ttcatcagaa agatccttgg ggtgggaagt cataaagcgg attcttttta aaccttcgat
    14281 ttcattaact tttcttaaaa gttgggcaaa actaattccg tcttttaagg tttttccata
    14341 agaattgaca ttctgtccaa gtaacatgat ttcttttaca ccatctgcaa ctaattcttt
    14401 gatttcattt aaaatatctt caggctggcg gcttctttct ctccctctaa cataaggaac
    14461 gatacaataa gtacagaaat tattacaacc atacataata ttaacgctgg ctttaaattt
    14521 atattttctg atacttggca gatcttctac gatatcttta tgctcattcc aaatattaat
    14581 aaccgtttct tttgtttcca gataggtttg aagaagttct ggcaatttat aaatattgaa
    14641 tgtcccaaaa ataatgtcta catgaggata ttttgatttt ataatattta agaccgtatc
    14701 ctgctgcatc atacaaccgc aaatggcaat gattaaatct ttattcatgt cttttaaata
    14761 ttttaaataa cccaattttc catagacctt ttgctcagcg ttctctctga cacagcaagt
    14821 attgtataaa ataaaatctg cttcattttc ctggtcggtt ttttgatatc ccatttgctc
    14881 taacatacca gctaattttt cggaatcatg ggcattcatt tggcagccaa aggtggcaat
    14941 ataatagtgc ttttgcttgg atgttttgga tttttccaag tcattttgca ttcttatttg
    15001 ttttatatat gtttcttgtc tttgcgcttc ctctgtggtc atcatactgg gtgtccttct
    15061 tttactcatc tattttaatc ctcctaaaac aatctctaaa ctcaaactta attttacata
    15121 atactgcaaa caaatgcaag taaagcaaca aataaaaaaa attctctatt tgcatagaga
    15181 ataatcatta aaatactaat gaataataaa tccaaacaaa atgcgatgca atgaccacca
    15241 tataccaata agagcataat tttaagtaga aaatgctctt tttgatttcc atgagacttc
    15301 ctttttttaa tcggttaaaa actaaaaaga gtaataagct tggtaaaata cctttaatga
    15361 taataaataa aggaggatta tgaagcagcc tagctaaaaa aggattaatc tcaacgccat
    15421 atccgttcgt tgtggcatat aaagtcagaa tcatatcgat aaaattaaaa acagctataa
    15481 aataaaaata cttttttgta taattaataa aaatttcttt agaaaattca ctcataagat
    15541 ttctccttag aataatatct ctaagcttat taatctccag agtgaatttt tctattcata
    15601 aattttactt ttattttatt gaaactcttc tgttaccgat tgtatatagt ctaagatttg
    15661 tctaatttct tcaggattat ctgatttaag actatcgatt ttttcattat aaaaattttt
    15721 aagatgaaca gtcagttcac cattggattc atcgctccga atacagcgaa aaccttcttt
    15781 atatttttct tttaattttt caaactgatt gttattcaaa atgactctcc cctttttgat
    15841 ctttttatta tcttgagcca tttttttaaa tctatacata tgtttaccat aataatttta
    15901 tgtaaatata attcgataat caatttagat tatttaaaaa gaataataag tatgatataa
    15961 tttaaatacg aatacaagcc ttggaggtgt tactatgtat gtaagaaacc gtatgaatac
    16021 caatgtcgtt acagtaactc cggatacccc tgttacgacc gttttcaatt tgatgatgga
    16081 acatggctac tctcaatttc ctgttgtaga gggaaatgag ttaaaaggtc ttatcacaga
    16141 aagagtatta aaagatgtaa gtccttcaaa agcaacttcc ttaagtatat ttgaattaaa
    16201 ttatctttta tctaaaacaa aggttaaaga tgtcatgatt acagacatta ttacagcaag
    16261 tcctgatatg ttaattgaag atgcggctgt tttattaaga agtaatgttg tcggctctct
    16321 tcctgtagtt tctgaagaca atcaattagt tgggattatt accagaacgg atattataga
    16381 tgcatttatc gaatttcttg gtaccaatga tcatggcaca cggatttctc ttgaagtgaa
    16441 aaatgaaata ggtacttttg caaaaattgc caatatcata aaagagcacg gaattaatat
    16501 tcatcatgta agtaattatg acatagacga taacaaatgc gaaatgatta tccgcttaaa
    16561 tacaacagac gttgaggcat tagttgattc tttaaagaat gaaggatata atatcttttc
    16621 ggttgtaaaa aaagaataaa aaacattcta taaaaaaaga aggctttatt gccttctttt
    16681 tttagcctaa ataagccttt cttacttcat cattacctaa taagtccttt gcattgcctt
    16741 caagaacaac ctttcctgtt tctaatacat aagccctgtg agcaatggac aaagccatat
    16801 tggcattttg ttctaccagc aaaatagtgg tacctgctgc attgatttct ttaataatat
    16861 taaatatttc ctgaaccaga ataggtgcca gccccataga aggctcatcc atcagaagca
    16921 gtttcggctc tgacattaaa gcacgtccaa tagccagcat ttgttgttct cccccgctta
    16981 aggttccggc aatctgtttt cttctctctt taagtctggg gaatttctca taaaccattt
    17041 caaaatttct tttgattgct tttttatctt ttttcgtaaa agcacccaac tctaaatttt
    17101 cttgaacagt catttcggca aaaactcttc ttccttcagg aacatgggca atacccagtt
    17161 gtaaaagctt gtgagcttca agatgatcaa tcttttgctt ttgaaaagat atttccccgc
    17221 tttttgcctt catcagacct gatacagtat gaaggatggt cgttttacca gcgccattgg
    17281 caccaattaa agtaacaatt tctccttcat taaccttaaa actgacgtct ttaatggcat
    17341 gtattgcatc ataatagaca ttaatatttt ttacttttaa catatagtaa cctcctattc
    17401 tcccaagtat gcgccaataa cttttttgtt ccgtttaatt tcctcgggag taccggttgc
    17461 aataatcatt ccataatcaa taacaataat tctttcacaa atgcccatca ctaagctcat
    17521 atcgtgttca atcagcagga tagcaatctt gaatttatct ctgataaaat gaatggtttc
    17581 cattaattct tttgtttctt gcggattcat acctgctgcc ggttcatcca ggagtaataa
    17641 tttaggatct gctgccaaag ccctggcgat ttccagtttt ctctgttgac cataaggaag
    17701 atttttagca gtggtataag ctgcatcctg catatcaaat aatttcaaaa gttccatagc
    17761 tttttcttca atttcttttt cttctttcca ataggcattt aatctaaaaa tgcctgctgc
    17821 tatggaatat ttcatcttat gatgaaaagc aatcttcacg ttttccaata cacttaaatc
    17881 tttaaacaat cttatatttt ggaaggtcct tgtaatacct ttttcaacaa tttcaaaagg
    17941 ttttttacca acgatattct ccccgttaaa atatatggtc ccttctgtag gaagataaac
    18001 gccagttaat aaattaaata ccgttgtttt tcctgcccca ttgggaccaa tcagtcccac
    18061 aagttccttt tcgtcgattt ttatattaaa atctccaact gctcttaaac ctccaaaggc
    18121 aattcctaaa tcttttgtct ctaataatgc catctgttat tcactccctt cgttcttaac
    18181 aggagtattg ccttgagatt tctttttccc tgataaaagt ccgttaagag aaaattctct
    18241 tttttccaat aaaccaccgg gtttaaagag catcattgca ataagcagca cagaataaat
    18301 aatcatattc caatcggcaa aacccattaa tagacgtggt aaaatggtta taataattgc
    18361 tgctaaaaca gaacccgtta agttacccat accgccaaga acgacaataa taagaatttc
    18421 tatagaacga ataaagttaa aatccttggg atctaatacc aaagtatggt gagcgaaaag
    18481 gcctcctgct acacctgcaa aaaaggcggc cagggtaaag gcaaaagttt tatagtattt
    18541 agttgaaatt ccaacagctt ctgcagcaat ttcatcttca cggatagaaa taattgccct
    18601 gccatgtctc gaattaatta aagtatagaa aatggttaca gtgataactg taataaaata
    18661 tacagtacta aagctggtaa gaggcagaat gcctgaaagc ccttttccac caccagttat
    18721 tttcatgttt aaaataataa cccgaataat ttctccaaaa cctaaagtta aaatagcaag
    18781 atagtctcct ctgaggcgga aaaccggaat agccatcaaa aaggcaaaaa aagctgccag
    18841 cagtccacct aagacaatag aaaccaatag taaaggcaaa tcaggtaaat tcattctggc
    18901 acttaaaata gctgctgtat aggcaccaat agccataaat cctgcatgtc ctaaagtcaa
    18961 ctgacctaaa taacctgtag ccagattcaa gcttacggct aatataatat taatgcccat
    19021 ctctattaat atgccttcat aataattatt tataaatcct attctgatga gcagaacaag
    19081 aatgatataa atcagaataa tgcttgttgt attgattata tttgctttta accgactgtt
    19141 catcctgctc acctacactt tctctttaac atttttaccg agtaatcctg atggttttaa
    19201 actgagtaca acaattaata taccaaatac aacagcatca gccaattgac tggaaatata
    19261 acctttggtc aaactttcgg caatgcccat aataaaacca cccagcatgg caccgggaat
    19321 aatgccaatt cctcctaaaa cagcagcaac aaaggctttt aaaccaggca taacacccat
    19381 aaaggggtca attaaaggat aagcaatact gtacaaaata cctccaacag caccaagagc
    19441 agaacctgtt gcaaaggtaa gggaaattgt agaatttaca ttaattccca tcaattgtgc
    19501 tgctcccgga tcttctgata cagcacgcat tgctttccct ggttttgttt tcttaataaa
    19561 taaatctaat aatatcatta ataaaacaga tatcagaacc gtaataaatg ttattctact
    19621 gatttgaaga ggacctaaag aaattggact tggatttata atttgaggaa atggaatggg
    19681 attttgtcca aatttcttaa agaatacatt ttctaagagg aaacttaccc caattgctgt
    19741 aatcagagca gataatctgg gagaatttct aaggggttta taagccactc tttcaattaa
    19801 aattccagac agcagacata aaataacagg agtaataagc gctactccaa taggtaaatt
    19861 aaatgtcaca atgcagacaa aagtaaaata agcccccatc atgataatat ccccatgggc
    19921 aaaattaatt aatttaatga tgccatatac catggtataa cctagagcaa ttaaagcata
    19981 tatactgcca acatgaatac cattaatgag ttgttgtaga aattttgcaa gcacatccat
    20041 gtttgtcctc cttcacttaa atgtagataa agggagaatt acttctccct ttattgagga
    20101 ttcactttcg tatatagtct gttttgacca ttctcaattt ttataatggc aacgtctttt
    20161 acaggatttc tgttttcatc ataagtgatg gttcctgtca cagaagtaat attggtttca
    20221 gccaaagctt taacaactgc atcataatcc gttgtacctg ctttttctat tgctgctgca
    20281 aggattttta cggtatcata tcccagagca gcaaaactta caggatcttc attgtatgtt
    20341 tctttgtatt tcttaataaa gttttgtact tcttcagcag tgtcatctgt tgaatagtga
    20401 ttgcagaaat agccaccttc aactgcttca ggagcaacgg ttaaaacgcc gtcccaacca
    20461 tctgcaccaa gaagcgttgc attaatacca acttctttta cttgttgagc aatcagtcca
    20521 accgtattgt agtaatctgg taagaataat acatctggat tttgagtctt tacattggta
    20581 agaatcgctt tgtagtcttt ttcgtcagcc ccatatcctt cataacttac aacagttcca
    20641 ccatttgctt caaaataatt tttgaaagct tcagcaaccc ctaaggagta atcgtctgct
    20701 gtattatata aaacagctgc tttacttgca ttcaaatttt caagagcaaa cttagccata
    20761 atttctccct gataagggtc aataaagcaa gtacggaaaa tgttagtacc agcagcagtt
    20821 acatctaatg ctgtcgctgt aggagttatc ataggaatac cttcttttgc tgccaaaggt
    20881 gtaatggcta atgtaggttt actggtgaca gcaccaatga tagcattcac tttattatta
    20941 ttaacaagtt tcttataagc attaactgcc tcagtagtat cccctttatc atcgtactca
    21001 atgtattcga ttttttttcc taaaatccca ccactggcat taatttcttc aatggctaat
    21061 ttagcaccat tacttgcagc aactccatat acagctacat ttcccgttaa tggggctatc
    21121 ccaccaattt taattacatc tgaatcagct gaaccggaat ccttctgtcc acaacctgca
    21181 agagcaccag ccagaagaac accagttaag gtcaaagaca ttaattttct taaccttctc
    21241 attgttctcc tccattcaaa tatttaatta atatattata ttgtttcata catgaaatta
    21301 tatatctaaa gcactgcaat ttcaagaata atttttaata gtttactgca atgcaatgtt
    21361 actattttaa aattatattg gagtcctctt taaattgttg tattatttaa tgtagaatca
    21421 aatgatactt tcataactgg ttaaagttta tatatcatta aaacttcaat atttaaagga
    21481 gatacttatg attaagataa agaaaaactg tcagttggat gctaaaggag cctttttggc
    21541 aaattataat ttcagcgact taattttttt tgatatagaa agtactggtt tttccattga
    21601 atattccaat gtatatttaa taggggctgt ttattatcaa aaaaaccagt gggtattaga
    21661 acagtttttt gctgaaaatc tttcagaaga aaaaaagata atagaagaat tttttacttt
    21721 ggcttcagct tataattaca tcatacattt taacggtgat acttttgata tcccatattt
    21781 agaaaaaaag gcttcattac atcatcttga agcacccttt aaaaaaataa ccagtatcga
    21841 tattttaaaa ttaataaggc cttatcaaaa aatacttggt ttagaaaatt gtaaattaaa
    21901 aacgatagaa cactttctgg acatcaatag aaaagatata tattctggca aagaactcat
    21961 tgaactatac caacagtttt gtaaaaaaca tgaccctaaa gtaaaagaaa acattttact
    22021 gcataatgaa gaagatttat tgtacctgat tccattgttg aaaatatttg acttaattca
    22081 gtttattaaa gatttaaaag taaatatttt agataatatt caagcctttg atataacagt
    22141 ggataatgat ttattgtttt cctttgcttt attaaaagct gctcctttat ctttacagtt
    22201 aaaaaatgaa aactgggcat gttcaataac aaaagacagt cttcatttgg attttaaggt
    22261 taaacttgta gaagatacaa tgtatcattt tttccccaat tataaagatt attattatct
    22321 aattgatgaa gacgaagcca ttcataaatc tctgggtgcc tttttaccaa aagataaaag
    22381 aaagcaggct aaagtctcca attgttatac aaaaagaaag ggactttttt tacaaacatt
    22441 ccagccggat attcgcttac ccgtttttaa aaaaagctat gattcaaaag aaatgtatat
    22501 tttattggat gataataaaa tcgatcacca aaaattgaat ttaaaagatt tagccctggc
    22561 ttttattgat tcacaaattc cacggtcttt ataaaaatca gcagcaaagc tgctgatttt
    22621 tatttataca gctggtttgt tgatggattg attaatttgt tcaatcgctc tgtatacgtc
    22681 ttcaatttta gccttaatag ccattaattc atcatcttct ttattattta gcagttcttt
    22741 atttaagctc ttcgttaaga aatacaattc ttctgataaa gcatccacct gagcaagtaa
    22801 ttgcaaacga aggaagtttt ctttgagggc accataaggc ttttgaggaa tcgctttatg
    22861 taaggttaat tcataattgt cgcctctttc agggatagct acatctacat taaactgttt
    22921 ctttataagt tttgctaaag ttgcttgcgc ttccggttca ccatgaacaa taaaaacttt
    22981 tttaggcatt ttggtaaact gctgaagcca gtttaataaa cctgcctgat cagcgtgtcc
    23041 tgaataacct tcaatatttt caattctggc attgactgaa atttcttctc ctaatatctt
    23101 aacttttttc gcaccatcta atattcttct tcccaaagtc ccaggagctt gatagcctac
    23161 aaataatatc gtacattctt ctctccaaag attgtgttta aggtggtgtt taattcttcc
    23221 tgcttcacac ataccactgg cagagatgat gatcttaggc tctttatctt cgtttaaagc
    23281 tttcgattct tcaggtgttt ttgtaaatgt taagctagga aagtctaagg gattgttgcc
    23341 atttttgata taatatcttg cttcatcatc ataacagtct aaattttttc taaatacttc
    23401 tgttgccgaa atagccaggg gactgtccac ataaacagga acggtgttta agaaattcac
    23461 atctgcttca tcaacttcat tatatttaaa taactcataa attatttcct gagtcctgcc
    23521 tacggcaaaa gaaggaataa taacatttcc gcctttatta atcgtttctt caataatatt
    23581 aagaagtttt cttgccttat tttcccgttc tttatgaatt ctgttcccat aagtagattc
    23641 cataataaga taatcagcac tatatattct ttcagggtct tttaaaatag gaatatcata
    23701 gttacccaaa tccccagtaa ataccagttt tgtttcctca ttattctcat taacccagat
    23761 ttccagaatg gatgaaccta acatatgacc tgcatcagaa aattttatgg ttatattgcc
    23821 tctaagatta acgacttcat gatatgcgac aggttcaaaa aatttcatac attttacagc
    23881 atcttcataa gtataaaggg gttctataag gggtttacct gcccttaatc tttttctgtt
    23941 cttccattca gcttccgttt cctggatata gccgctgtct ggaagcatta tggcacacaa
    24001 atctgctgta gctctgctgc aatagatttt tcccttaaaa ccatctttac agagtttagg
    24061 gatttttcca ctgtggtcaa tatgtgcgtg ggttagaagt aaataatcaa tttttgttac
    24121 atcaaaaggc cagggttcat agtttaaatt ttcttcctga tcagccccct gaaacatacc
    24181 acaatcaatg aggatatgct tgttgttggc ttctagtaaa aaacatgagc ctgttacggt
    24241 tttagcagct cctaggaaag tcagcttcat acttatcccc catttcaagc taaacttttt
    24301 acgacctcta ctatgatttt aacaaccagt ccaatcatta ctccttaagg acttttatat
    24361 ttttagcaat ataatcgatg caggatataa tggtaaataa tataaccgcc cccattagaa
    24421 gatattctaa ataattaaac aaagtcgtat tgatatttaa taaaacgatg ataatcatca
    24481 gcatctggct aactgtttta atcttccccc accaactggc ggcaatgaca attccgtctg
    24541 aagcagctat gagtctaaat cctgtaataa tgaattctct gcttataatc agaatagcta
    24601 tccatgcaga taaatctcct aattcgacta agctgattaa agctgaagta accaaaagtt
    24661 tatctgctaa aggatccata aactttccga aatttgtaac caaattattt tttctggcaa
    24721 tatagccatc taaataatcg gtaaaagatg ccaaagcaaa aataataacg gcaatgattc
    24781 ttttttgagg atctggaagc cagttggtca aaagaatcaa aagaaaaagt ggaatcatta
    24841 tgactcttaa aatggttaac ttattcggta aattcatcaa taatctctcc tactaaatca
    24901 tattcttcag cctctgtaat cttaaccttg acaaattctc cagaaagcag ctctcctttt
    24961 gttttaacaa aaaccatgcc atcaatgtcc ggagcatctc tgtaagttct tccacaataa
    25021 acatcttcac cagcgatttt cccatcaatt aatacttcta agtgttttcc aataaattct
    25081 ttagattttt cagcacaaat ttgtctttgt aatgccataa gaatattttt acgttgctct
    25141 ttaatttttt taggaatttg atccttcaat cttgatgcag gggttccttc ctcttttgaa
    25201 taactaaata cgcctaaacg gtcaaatttc atcgttttaa cgaattcttt catatcttca
    25261 aattcttctt ctgtttctcc ggggaaacct gtaataagtg tggttctaag acaaatatca
    25321 ggtattttat cccttaattt attaattaat tgaatgagcg cttctttagt actttttctt
    25381 cccattcttt ttaaaacagc attattggaa tgctgaatag gcatatctaa ataattacat
    25441 attttttctt ctgttgccat aacttcaatc aaatcatccg ttatttcttc aggataggta
    25501 tataaaagcc ttatccattt taaatcttct attttacata attcttttaa aagtatggga
    25561 agacgttttt ctccatataa atcaacgcca tagcgggttg tatcctgagc cactataata
    25621 agttcctgaa ccccttgatt gactaaattt tttgcttcct taattaaact ttccatggaa
    25681 cggctcctgt atttccctcg tattttagga ataacacagt aagtacaagc attatcacaa
    25741 ccttcagcaa tttttaaata ggcatagtaa ccagcagtcg ataagattct ctctgaatca
    25801 tcacttaagc ttatattgat atcagaaaat ttttcaactt ttttccctgc cataacttct
    25861 tttataactt ctacaatttc cctgtaactg gctgtaccga caatagcatc tactttaggc
    25921 atttccgata aaatttcatc cttataacgc tcagccagac aacccgttac aataattgct
    25981 ttgcaattgc catctttttt gtattccgca gcttctaaaa tattttcaat gctttcttct
    26041 tttgcatctt gaataaagca acaagtatta atcacgatga catcggcctg gctttcatcg
    26101 gatatgagtg aaaaaccagc ttgattgagc aagtaaagca taacttcact gtcaactaaa
    26161 tttttatcac agcctaatga aataaaacta atttttaatg tcaaaataaa tctccttcct
    26221 ggtctacatg attgcagctt ttacacaatt tttcattaac atggcaatgg tcatgggtcc
    26281 tacacctcca ggtacaggag taatagccat cgctttatat ttacagtttt caaagtcaat
    26341 atccccacat aatttgccat tttctaaacg gttaattcca acatcaatta aaacagcacc
    26401 atcttttacc atatctgctg taataaaatt tgcttttcct atggcagcca ccaaaatatc
    26461 tgcttcttta cagatttctt ttaaattttt cgttctggaa tggcaaattg taacggttcc
    26521 attatttctt aataagagat tggcaacggg cttgcctacg atattacttc ttccaacgac
    26581 aacacatttt tttccttcaa tttcaatctg acttcgttta attaattcaa taatacctgc
    26641 aggtgtacaa ggcagaaaac catttaattc accaatagaa atcatgccta cattgtaagg
    26701 atgaaaacaa tctacatcct ttgaaggatc aatagaaagc aggattttat tctcatcaat
    26761 atgttttggc aaaggcaatt gaaccaatat gccatgtact tctttcgttt gatttaaatc
    26821 ctttatcagt ttaagcaatt cttcttcact ggtttcttca ggcagttcat aagaaaagga
    26881 acggattcct atatattcac aagctttttt cttattggat acataaactt tagaggcagg
    26941 attattccct accaggatta cggccagtcc aggctcgata ccttttttct ttaaatcttc
    27001 cacttctgct tttaattcat ccttaatatc ctgagaaatc ttttttccgt caataattaa
    27061 agtttccata tttcatcctc ctaaaacaat cctgtaattt gtcctttttc gttgacatct
    27121 atttttatgg cagcaggttc tttgggaagc cctggcattg tcatgatatc tcctgtaaga
    27181 gccacaataa agcctgctcc agcagaaacc cttaattccc taacggtaat ttcaaagtct
    27241 tttgggcatc caagtaaggc tggatcatca gataaagaat attgggtttt tgcaatgcaa
    27301 ataggcattt tatcaaactg gttcctttca atgttttcta aatcctttaa ggctttggat
    27361 gtataattta cctttgaagc cccataaatc ttttgagcaa tgctttcaat tttttctttc
    27421 acaggcgcat tatggtcata aaggacttta aactcagatt ttttattttc caaagtatca
    27481 atcacttttt gagccagttc tattccgcct gctccaccct tttcccatac ttcagaaatt
    27541 gcaaaggcac agcctgcttt ttcacattct tcttttacat aatcaatttc tgcctgggta
    27601 tcggttaaaa aggcatttaa ggtgaccaca acaggaactc cgtaaagttt aatattctca
    27661 atatgttttc ttaagtttgc aaagcctttt ttaagggcat cgagattttc gatgcttaat
    27721 tgattctttg gaataccacc attgtatttt aaagctctta tggttgcaac caatacaaca
    27781 gcatctggct ttaagccagc aaaacggcat tttatatcaa agaatttttc agcacctaaa
    27841 tcggcaccaa aacctgcttc agtgacaacg atatcagcca gttttaaggc taattttgta
    27901 gccctgatac tgttacaacc gtgagcaata ttggcaaagg ggcccccgtg aataatcact
    27961 ggtgtgtttt ctaaagtttg aaccaaattg ggtttaatag cgtctttcag taaagctgtc
    28021 attgcaccat ttgctttaag atctttagct ctgacgggct ctccgttaaa attataagca
    28081 acgataattt cacccaggcg tctttttaaa tcctctaaat ctttggccag gcaaagaata
    28141 gccattactt cagaagcaac ggtaatcata aaaccgtctt ctctgggcat gccatgtgcc
    28201 tttccaccta aaccaacaac aatatttctt aaagcccggt catttaaatc gacaactctt
    28261 ttccaaagga tttgtctggg gtcaatttgc aattcattac cttgttgaat atgattatca
    28321 atcatggcag aaagtaaatt atgggctgta gaaatagcat ggatatcccc ggtaaaatgc
    28381 aaattgatat cttccatagg aaccacttgg gaataaccac ctccggcggc cccgccttta
    28441 acacccatac aaggtcctaa agaaggctct cgaagggcaa taaccgcttt tttacccatt
    28501 ttacacaggg cttgtcctaa ccccacggag gtagtcgttt taccctctcc tgcaggtgtg
    28561 gggttaatcg cagtcactaa aaccaatttg ccatctggat tatttttaac tttttcaaaa
    28621 aggctttctg aaatttttgc tttatatttt ccataaagct ctaaatcatc aggctcaata
    28681 tccagttgtg ctgcaatttc agtaattggt ttcatctgcg cttctaaagc aatttcaata
    28741 tctgttttca taagaaccct cctatatttt aatagattta attgttgtta gaattttgct
    28801 gggattttag cttcatttgc ctgaattctt cctggctgat taaaaccttt ctgggtttac
    28861 ttccttcttc aggaccaacg acaccccgtt cttccatttc ttccatcaat cttgccgccc
    28921 tgttaaagcc aattcttaaa tatctttgca acattgaaat agaagctttt tctttttcta
    28981 caacgatttc tatggctttt tcaagatatt catctaaatc ttcatctggt gtaaagttat
    29041 taatatcatt gatgttttca ataatatcct ggtcatattc cgcttctttt acttgtttaa
    29101 tactgttaac cagggcttct acttctgagt ctgaaataaa tgccccctga attcttacag
    29161 gcttgttggc tccaacagga taaaaaagca tatctccttt acccaataat ttttctgcac
    29221 cagtcatatc cagtatggtt ctggaatccg ttcctgaaga aaccgcaaaa gctagtctcg
    29281 aaggaatatt ggcttttatc agaccagtaa tgacatccac tgaaggcctt tgcgttgcaa
    29341 tgactaagtg aatgcctgct gctcttgcca tctgagcaag ccggcaaata gcatcttcta
    29401 cttcattcgg cgctgtcatc atcaggtcag ccaattcatc tataataata accatttgtg
    29461 ggagtatttc taaattcgat tcttcttttg cacttattaa atgattatag ccttttatgt
    29521 ctctgacatt gtattcagca aatagtttat aacgattggt catttcctga acagcccaat
    29581 taagagctcc tgccgctttt ttaggatcgg ttacaacagg aatcatcaaa tggggaatac
    29641 cattataaac actcagttca acaaccttgg ggtcaattaa aatcagtttt acttcatcag
    29701 gtcttgcctt atagagtatg ctggttataa gagtattaat acatacgctt ttccctgagc
    29761 ctgttgctcc tgctattagc aaatgaggca tttttgcaat atccgcaaca acaggattac
    29821 ccgcaatatc cttacccaat gcaaaagcta atttagacgg aaatttttga aattcttcat
    29881 cttcaatgac ttctctaaga taaacagcct gtgctttttg attaggtact tctatcccta
    29941 cagcagcctt acctggtatg ggtgcttcta tacgaattcc tgatgcagcc aaattaagcg
    30001 caatatcatc agctaaactg acaattttac ttactttaac cccttgtctg ggttgtaatt
    30061 cataacgggt tacagtcggt ccacggctaa tttgaacaac ttttgcttct acaccaaaac
    30121 tgtataatgt tttttctaat tttttggcat tatccaacat cttatttttt gaatctgtcg
    30181 tgggtgaata aggatttttt tgcagtaaat caattgatgg aaaatgataa tccacgggtt
    30241 cttttggttc ctttttagtt gaaatgcttt tttctatgga aagaatttca ttattattat
    30301 tatttttctt attttccttt tctttagctc tattgatttc actataatct tcttctgtaa
    30361 gatttgctgt gatttctaac tgggtctctt catcgtcttt tttttctgta aaatctaaga
    30421 cggttattaa atcatctgtt ttttcaatgt aatctttttc ctgactttta ttttggttct
    30481 ttattttatc ttttttcttt ccacttttta aatgaaataa ttttttaaag aattgactta
    30541 ttcccttgat aatcatttta aatttttcta tgaaagaatt atccgttaat aaaataaata
    30601 aaacaataaa aagtgaaata atgataatat agccgccata tctgccaact aatttaagta
    30661 aaagatttgc cagtaaggct cccaaaagac ctccgttatt ccaatttcca atatgaaagt
    30721 aataggtcat aaaagaccac agactcatat cttttgacgt aataagcata tcaggttcta
    30781 aatacaaaat atgtaaaaaa gttgataaaa gaataataat cgctgaagcc gcaaacaatt
    30841 tgctttttgt tatttttcct tgttttttaa aaagaataag gatgcctata acagtaaaaa
    30901 caacaggtaa aaaataagcc cctattccca ataaacctag tgacaattgc tttatccact
    30961 ctccaattat acctccttgt tttgtataaa tacccactgc aaggattaaa gcaatagtta
    31021 caataatgat tccttttatt tcttgggaaa gcggacttcc tgttttttta aactgattgt
    31081 ttttttttct gttcgtcatt ctttttccca tattatgcag tcccctcttt aaataaaaag
    31141 tgctagcatg agctaacaca tatatttatt atagcatgtt tattgaattt tgtgataaag
    31201 atttatcatt tattaaaaaa tctgttcaat atgatatttt tattgtattc ccaggctgta
    31261 attcatcatt taaataatca tagggatttg ttgacagaat tctttcaata atatagtttt
    31321 tattttcatt atagtatacc tgaagcaaac ttcctttata atagatttct tcaaattgaa
    31381 tgtttctatc tctctctgaa tcagcaaaaa tgatatcttg agaaatgaca gagtaaatca
    31441 tttcgcttcc tccttatcat tgttttcatc gattagttgg tagagttttt taatggcctt
    31501 atcgagtcca ccgacttcat cgattaattt ctcttttaca gcttcttcac caactaatat
    31561 ggttcccaca tcttttgcca gcatacctgt atccaacatt aattctttaa aacgcttctg
    31621 gctgataccg gaatttctgg ccacaaattc gacaattctt tcctgcattt tttcaaaata
    31681 atcaaaagtc tgagggatac ctatgacggt tccgctcatt ctaacagggt gtatagtcat
    31741 agtcgcagtc gaagcaataa aagaataatt tgcagccact gccaaaggaa caccaatgga
    31801 atgaccacca cctaaaacta aggaaacaga aggtttccct aaagttgcaa tcatttctgc
    31861 tatagcaagg ccagcttcca cgtccccgcc aacggtattg agaataacca gtaaaccttt
    31921 tatgtcttca ttatctctaa tagcaactaa ttgaggaata acgtgttcat attttgtggt
    31981 tttgttttgg ggaggaagga gaatatgacc ttctatttgt cctataatgg ttaaacaatg
    32041 aatttttgat ttttcatctt ccttgttttc ttctatgggc atttgaccaa actcttttat
    32101 tgtttctgtc atatcacttt ggtttttttt aagctcttcc gtctttttca ttaaaacgcc
    32161 acccatctaa agaaagtttt tttagtattt ttcctctgat tcgtaataaa aatacataaa
    32221 aaaaagattc cataggaatc catataaata ggatagatat gattatcctt aataataatt
    32281 gaattacaat tttataataa caggctgaac ttgttttttc tgcaatgcct gttctactgt
    32341 attcaaaata agattcatat cagacataat tgaatttggt tttttctcac tgttatcctg
    32401 tcccataggc acaaaaaata tgtttttaga attcattaag atgccgatat ttttcatgtt
    32461 taatcctaaa ccatcattcg ttgctacggc caaaacaaca ggtttttcgt ttctcaggag
    32521 tgcttttgcg gccataagga caggcgtatc tgtaatggca tttgccaatt ttgctaaggt
    32581 atttcccgtg cagggggcaa tgactaaaat atcaatgaga ttattgggac ctaaaggttc
    32641 tgcttctaca atgcttgtta tgattttttt tcctgtcatt ttttcaattt tttccttatg
    32701 ttcttcggct tttccaaatc ttgtatcggt gcttgaagca ttgtttgaca taatcggaaa
    32761 tatttcagcg ccttcatcta taagtgtctg aatagctgga atgactttat cataagtaca
    32821 ataggaacca cataatgcaa agccaatttt tatgcctttc aatctattca tattattacc
    32881 cccatatccc taatgacgtt taatattgtc tcataaataa ttaaagccgc actttctggc
    32941 gctactttac ctgggaggct tggggctaaa atagcttgta ttcccagttc ttttgctgca
    33001 ctgtaatcaa cgccgccagg ttttgaagca atatcaatga ttaaagcgga tttttttatt
    33061 ttttgaagaa ggtctctatt taaaattaag gcaggaatgg tgttaaaaat aaaatcataa
    33121 tcccctataa atttttctaa atcctgaaga taaagagctt tgtaaccata actttttatt
    33181 aaagccaaat cttcagcttt tctggcttct acagttaggt ctgcatccag accttttaat
    33241 ttgtgggcta aaactttacc acatttccca aagcctaaaa ccaggcattt gcttccgtga
    33301 attgttattt cactgtttcc catagcatat aatatagccc cttctgcagt aggaattgca
    33361 ttaagaatgg ctaattcctc tctttctaat aaatcaataa ctttaaatgg atattgctct
    33421 gcaatctctt taatttgcgg cgtaatactg cctgccataa agattttatc ttcagaaata
    33481 tttttaaata tatcttcaag cataatgctt gaatccagat aatcagaata tacagtttta
    33541 ttatcccgtg aacaaggaat aggaccaaca ataatatcgg catcagaaat gagttctctt
    33601 atgttattaa aaagatttac cccactatct atatttttat tatcaaatcc aaacatattg
    33661 acctggtaac ctttacttgc taaaaggtta attaatttaa tttgtctgag gtcaccccca
    33721 atgactgcaa agcggatacg atgatatgtc aattctatcc ctcctgctaa aaatattggg
    33781 ttatcagtta gtatagttta tgtaaaaaac catgcttttt gtgcttgtct gacataaaag
    33841 caataaaaaa acatatgaac ctttttattc atatgttttt acctgcctgt atgaccgaag
    33901 cctcctgaac ctctttcgga tctatctaat tcttcaactt gtacccattc aacagattca
    33961 attttattaa ttaccatttg agcaatccgc tctcctcttt taatgataaa atcttctgat
    34021 ccaaagttaa tcataataat ttttatttct ccacgataat ctgcatcaat cgttccggga
    34081 ctattcagaa ttcctattcc atatttatag gccaaaccac ttctaggccg gatttgtgct
    34141 tcataacctt taggcaattg tatggcaata cctgtaggga ttaattttat atctcctttt
    34201 tttaagacga catcttcttc aacattggca taaagatcca tccctgcaga ttgctctgtc
    34261 atataagttg gcaatggaag atcttgtgca ttttctactt ttttaatgaa tacttttaat
    34321 cgatcagaca tagttctttt atctccttaa gcttattatg atcgattttc ccaacaatag
    34381 atatgcttat tttagaaaaa tcaaaaattt gttcgataag ttccgtaatt ttgtcttgag
    34441 taacagcatc tacactttcg ataatttcat caggggtttt tattttattt agtattaatt
    34501 gtgactttcc aatactggac atccggctcc cagtgctttc taatcccaga atataattgc
    34561 tctttaactg ctccttcgtt ttatttaagt cttcatggga tattttttct tttttaagta
    34621 aatgaatttc ttccataatt agtttaataa cttctatggt ttgagaagga ttcattcctc
    34681 cgtaaattgt aaaaagtcct gtgtctttat aatttgtagg ataggaatag attgaatatg
    34741 ctaaacctct ttcttccctg attttttgaa ataatctaga actcattcca cctccgaaaa
    34801 tcgtgtttag tgtaattaag tcatatattt tttcggaacc ggaatgaatt cctgggaaat
    34861 taatacaaac atgaacctgc tcgatgtctt ttaattttgt aatcaattga ggcttataat
    34921 ttggaatttt atagacataa tcatcctttt cttttgcctg ccatgatgca aatttttcct
    34981 caatttcagc aaatatttca ttataattaa aattgccagc cagagcaatc actgtatttt
    35041 caggccgata tcttttgtgg taataagaaa ataatctttc gctgtcaaat ctgcttatac
    35101 ttttagaact ccctaaaata ggctgaccta atgaactttc ttcccaaaca gctgtttgac
    35161 ttaaatcatg gacaagttct tcaggagaat cttcatacat attaatttct tccaatacaa
    35221 cttgcttttc tttttctatt tcccgattat caaaagttga gttaaaaaac atatccgaca
    35281 aaatatctaa agcttgacta aaatgattgt ccagagcacg gaaataatag caggtatatt
    35341 cttttgtggt ataagcatta agctggccac cgattctgtc catttcatcg gctatttgct
    35401 ttgctgtccg attactggtt cctttgaaca tcatatgttc aataaagtga gatataccat
    35461 tgttttcttt tttttcgtgt cttgaaccat taccaaccca tacgccaaaa gcaatggatc
    35521 ggacagaggg aattttttct gctactatag ttactccgtt agcaagcttc ttttttttga
    35581 acatataagt tctccttttc tttttctaag ataactttag tatctcttct ttattcttct
    35641 ttttcttctg attttgcaag ggtatctttt cttgataaat tgattcgtcc ctggctatct
    35701 atatcggtta cttttacaag gatttcatcg cctactttaa caacatcttc gacatttttt
    35761 atatgctcat gggcaagttt tgaaatatga accaagcctt cttttccagg agcaatttca
    35821 acaaaagcac caaagtttgt agtttttaca acttttccta aatagatttc tcctggctga
    35881 atatcttttg cgatgctttc aataatcgat acggctcgtt tagtcttttc tttatccaca
    35941 ccacagacat aaattcttcc gtcatcttct atgtcaattt ttacgcctgt ttcatcaata
    36001 attttattaa tcgtcttccc acgaggtcca atgatttcac tgattttttc cggatcaacc
    36061 gttgtctgca aaatcagtgg cgcataaggt gaaagtgttt ttctgggttc agggattgtt
    36121 tttaaaatca cttcatccag aatataagtt ctggctcttt tagtttgata taatgctttt
    36181 tcgataattt ccctatttaa gccacggatt ttaatatcca tttgaattgc tgtaatccct
    36241 ttatgggttc cggcaacctt aaaatccatg tctccaaaaa agtcttctaa accttgtata
    36301 tctgttaaaa gaacaaaatc atcaaggctt tcacctgtta ccagaccaac ggatattcct
    36361 gctacaggtg ctttaatggg taccccagct gccatcaatg ataaggtact gccacagaca
    36421 ctggcttgtg atgtagaacc attagaactt aatacttctg ataccaatcg gatagcgtaa
    36481 gggaattctt cttcacttgg tatcactgga agcagagctt tttctgctaa agcaccatgg
    36541 ccaatttcac gtcttcctgg tcctctggaa ggttttgttt ctcctacact gaaagagggg
    36601 aaattataat ggtgcatata acgtttggtg tcttctaaag catctaatcc atctaaaatt
    36661 tgaacgtctc ctaaagcacc taacgtagtt accgttaaaa cttgagtttg acctctttta
    36721 aataaagctg aaccatgggt tctgggaaga atgtcaattt cagatgaaag aggtcttatt
    36781 tcttcaagtg ctcttccatc aggtctttta tgttttctta aaatcatatt tctgacagtt
    36841 tctttttcaa acttactgat agcttctaca atataaggct cataatcagg atttttttct
    36901 aaaaagtgag cagtcacttt ttcagtcagt tcttttatct gaatatctct ttgctgtttg
    36961 tcatcggtaa agactgcctt ttccatttct tctgtaccaa tgaattcttt tatttcttca
    37021 taaagttcac aaggcacctc atgtttttca tagtcgtgtt ttggtttacc acattctgct
    37081 tgtatttctt caatgaattt tacaattttt tgattttcct catgagctaa aaaaatcgct
    37141 tctaccataa ggtcttcttc gatttcatcc gctgcagctt ctatcatcat aaccctgtct
    37201 ttagtggaag atacggttaa agaaagtctg ctgatttctc tttgttctgc tgtaggatta
    37261 atcacaaatt tgttattcac ataacctaca ttgacagaac ccgtcggtcc gtaaaaaggt
    37321 atatcggaaa tagaaagagc aacggatgaa ccaatcatgg ctgcaatttc aggactgcag
    37381 tcctggtcaa cggatagtac ggtagccaca acagaaacat cattacgata atcagaaggg
    37441 aataaaggtc gtataggtct gtcaatgact cttgctgtta atatagcttt ttcagtaggt
    37501 tttccttctc ttcttatgaa acctccggga attttaccaa cagcataaag tctttcttca
    37561 taatctacac ttaatggaaa aaaatctacg ccttccctgg gtttatctga agcggttgca
    37621 gtgactaaga caacagtatc cccatatcgg atcaatgcag caccattagc caattcagca
    37681 actttaccta tttcgactgt aagctttctg cctgcaagat ccacactgta tatttttgac
    37741 ataaaaaata ttactccttt ctacttagcc tatgtattat ttagtattaa aaaataatga
    37801 catgcttatt atagtattat tttgcaatat aatctaatta tactacaagg aagcaagtca
    37861 attccattat catatcatca tactcttttg caaaggcttt atgtcaatgt gtaaaaatct
    37921 tctgtttcaa gttaaaagag cggattactc cgctccttaa attattattt tctgatgcca
    37981 agctcagaaa taagttcacg atacttgtca atatcttttt tcataagata atttaacagg
    38041 cctcttcttt gtccaaccat cttaagaaga cctcttcttg agtgatggtc tttcttatga
    38101 gttttcaagt gctctgttaa atgattaatt ctgtaagtca acagagcaat ctgtacttcc
    38161 ggagaaccag tatcgttttc gtttctacca aattttttaa taatttctct ttttgtttct
    38221 ttatccattt ttaaaatcct cctttttatt tattagcacc ttttgctagg aataggccgg
    38281 agtttccgta atccgagcaa aggttataca ccaaatagat tatagcatta tcttcttatt
    38341 ctgtaaactt aaaatttaaa tttaagtaaa attaaatcat tataagtaaa atatttaa
//
LOCUS       DAOSUD010000011        36880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_790_length_36880_cov_27.435791, whole genome shotgun sequence.
ACCESSION   DAOSUD010000011 DAOSUD010000000
VERSION     DAOSUD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 36880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36880
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_36880_cov_27.435791"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..274
                     /gene="rpmG"
                     /locus_tag="PLG49_02750"
     CDS             125..274
                     /gene="rpmG"
                     /locus_tag="PLG49_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ16265.1"
                     /translation="MRVKITLACTECKQRNYNTTKEKRKHPDRMEIKKYCRFCRTHTL
                     HKETK"
     gene            306..488
                     /gene="secE"
                     /locus_tag="PLG49_02755"
     CDS             306..488
                     /gene="secE"
                     /locus_tag="PLG49_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOQ16266.1"
                     /translation="MKERFLQVKGEFKKIIWPNKKDLVKQTTTVIVTSLLTAGIVFVI
                     DFAYSRGLDKLMEILK"
     gene            501..1019
                     /gene="nusG"
                     /locus_tag="PLG49_02760"
     CDS             501..1019
                     /gene="nusG"
                     /locus_tag="PLG49_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOQ16267.1"
                     /translation="MSEDAKWYVVHTYSGYENKVKANIEKVIENRNMQDLICEVVVPL
                     QDTVEIKNGKRKTVQKKIFPGYVLVKMHMTDETWYVVRNTRGVTGFVGPGSKPVPLTE
                     EEIRGMGIDLPQAKIDFEVGDTVKVITGPFENSVGVINHINENKRTVTVRLSMFGRET
                     PVELDFSQIQKL"
     gene            1100..1525
                     /gene="rplK"
                     /locus_tag="PLG49_02765"
     CDS             1100..1525
                     /gene="rplK"
                     /locus_tag="PLG49_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ16268.1"
                     /translation="MAKKVIGMIKLQIPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTAKQAGYVIPVVITVYQDRSFTFITKTPPAAELLKKACKIEFGSGAPNKEKVATISR
                     EEVKKIAELKMPDLNAGSIEAAMSMIEGTARSMGIVVKD"
     gene            1604..2299
                     /gene="rplA"
                     /locus_tag="PLG49_02770"
     CDS             1604..2299
                     /gene="rplA"
                     /locus_tag="PLG49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ16269.1"
                     /translation="MKRGKKYIEAAKLVDRTTLYEPKEAFDLVQKIATAKFDETVEAH
                     IRLGVDSRHADQQVRGAVVLPHGTGKSVKVLVFAKGDKLKEAEDAGADYVGGEELIPK
                     IQNEGWLDFDVVVATPDMMAVVGRIGRVLGPKGLMPNPKAGTVTMDVAKAVKDIKAGK
                     IEYRLDKTNIIHVPIGKVSFGTEKLLDNFQTLMSAIIKAKPAAAKGQYLKSISVSSTM
                     GPGIKINPLKVEA"
     misc_feature    2387..2532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2555..3064
                     /gene="rplJ"
                     /locus_tag="PLG49_02775"
     CDS             2555..3064
                     /gene="rplJ"
                     /locus_tag="PLG49_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ16270.1"
                     /translation="MAKIEKKQEIVNEIKEKLSKSASVVLVDYRGLTVEQDTELRRKF
                     REAGVDYKVYKNTMMNFAIQDTEFEPLKDYLEGPSAVAFSYDDPTNGPRIIDEVASKY
                     NALEFKAGIVEGRFYDTAGIAKVAKIPPKDQLLSKLLGSFNSPIASFARVIKAIADKK
                     AEGAEAVTE"
     gene            3097..3483
                     /gene="rplL"
                     /locus_tag="PLG49_02780"
     CDS             3097..3483
                     /gene="rplL"
                     /locus_tag="PLG49_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ16271.1"
                     /translation="MAKLTVQEILDAIDELTVLELNELVEKCEEKYGVSAAAGVVVAA
                     AGAAAGGAAAEEKTEFDVELTALNDASAKVKVIKVVRELTGLGLKEAKDLVEAVPKVV
                     KEGVSKEEAEEVKAKLEEAGAKVTIK"
     gene            3764..7660
                     /locus_tag="PLG49_02785"
     CDS             3764..7660
                     /locus_tag="PLG49_02785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ16272.1"
                     /translation="MKKNRIHPVPAGKGVRMSYARMEEVLEMPNLIEVQKNSYKWFLE
                     EGLKEVFQDISPITDYSGNLMLEFVDFSLDENPKYSIEECKERDATYATALKAKVRLY
                     NKETEEIKEQEIFMGDFPLMTETGTFIINGAERVIVSQLVRSPGIYFDVDYDKVGKKL
                     FTATVIPNRGAWLEYETDSNDVFYVRVDRTRKVPITALIRALDIGSDERIIELFGEEP
                     KILSTLEKDNTKTHEEGLIEIYKRIRPGEPPTVDSAKTLLYNMFFDPKRYDLAKVGRY
                     KFNKKLAFRNRIRNFILAESVVDSTTGEVIAEAGERITPELADAIQDLGIQSVLIKVP
                     HHDKPVKILSNLTVNIEKHIDIDAEELGIKDRVYYPVLKELMEKYEDLEELKQAIKEN
                     IQRLIPKHITKEDIMASINYGIHLEYGIGNVDDIDHLGNRRIRAVGELFQNQFRIGLA
                     RMERVIRERMTIQDLEAVTPQNLINIRPVSAAIKEFFGSSQLSQFMDQTNPLAELTHK
                     RRLSALGPGGLSRERAGFEVRDVHYSHYGRMCPIETPEGPNIGLINSLATYARINEYG
                     FIEAPYRIVDHSTGIPIVTDKVVYLTADEEENYTVAEANTPIDEKGSFINKKVTGRHK
                     RETVEVDPSKIDLMDVSPKQLVSVATAMIPFLENDDANRALMGSNMQRQAVPLLVTEA
                     PIIGTGMEYRAARDSGVTVIAKKAGIVEKVTATEIVIKSDDGERQTYKLQKFVRSNQG
                     TCINQRAIVDKGQRIEAGEIIADGPSTDHGEIALGKNVLVGFMTWEGYNYEDAVLLSE
                     RLVQEDVYTSIHIEEYEAEARDTKLGPEEITRDIPNVGEDALKDLDERGIIRIGAEVR
                     SGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGESGIVVDVKIFTR
                     ENGDELSPGVNQLVRCYIAQKRKISVGDKMAGRHGNKGVISRILPVEDMPYLPNGRPL
                     DIVLNPLGVPSRMNIGQVLEVHLGLAAKILGWKIATPVFDGANEIDIMDTLDLANDYV
                     NMSWEDFEKKWGETLHPEAMKKLEENLDQREEWKGVPINRTGKIPLRDGRTGEYFDNP
                     VTVGYMHYLKLAHLVDDKIHARSTGPYATITQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAYTLQEILTIKSDDVVGRVKTYEAIVKGENIPEPGVPESFKVLLKELQSLCLDVKVL
                     LDDETELEIKESVDDIDELTVNIEGTEDGQDYLVGHSAKAGHLIDDDIEDEDIEDDED
                     ISEDIDDDFFEPSEEEDEMDVLDLFDSDDDIDFETE"
     gene            7677..11204
                     /gene="rpoC"
                     /locus_tag="PLG49_02790"
     CDS             7677..11204
                     /gene="rpoC"
                     /locus_tag="PLG49_02790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ16273.1"
                     /translation="MPTVNMDQSVFFDALKISLASPDKIREWSRGEVKKPETINYRTL
                     KPERDGLFCERIFGPSKDWECHCGKYKRIRYKGVVCDRCGVEVTKSKVRRERMGHIEL
                     AAPVSHIWYFKGIPSRMGLILDMSPRALEKVLYFASYIVLEPGDTGLQYKQLLTEKEY
                     RDACEKYGANAFRAGMGAEAIKELLCAIDLEKESQELKKALKETTGQKRVRIIKRLEV
                     IEAFRKSGNRPEWMILDVIPVLPPDLRPMIQLDGGRFATSDLNDLYRRVINRNNRLKR
                     LLDLGAPNIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPSLKIYQCGLPKEMAIELFKPFVMKKLVEDGLAHNIKSA
                     KRMVERLQSEVWDVLEEVIKEHPVMLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLVCT
                     AYNADFDGDQMAVHVPLSVEAQAECRFLLLSPNNLLKPSDGAPVTVPSQDMVLGTYYL
                     TLEKDGEKGEGSIFKDENEAIMAYYNKYISLHAKIKVRRTKEINGEKKSAIVNTTVGR
                     IIFNEAIPQDIGFVDRTKEENLFKYEIDFLVDKKALGKIIDRCINKYGVTNTAVVLDK
                     IKDLGFKYSTIGAITVSVSDMEIPREKQDILNEAETTVDEVTKLYRRGLMTDEERYQK
                     VIETWNKANEKITDALLKSLGKYNNIYMMAHSGARGSINQIKQLAGMRGLMASATGKI
                     IELPIKANFREGLSVLEYFISANGARKGLADTALRTADSGYLTRRLVDVAQDVIIREV
                     DCCSNEEEVPGMWVKAFKDGEEVIESLQDRIRGRWLLEDVIHPETGEVLAKANTMITP
                     DQAEAIEKAGVKKVYIRTVLTCRSKIGVCSKCYGANMASGRLVRVGESVGIIAAQSIG
                     EPGTQLTMRTFHTGGIAGDDITQGLPRVEELFEARKPKGLAIIAEFAGKVQINDTKKK
                     REIVITNEEAGESKSYLVPYGSRIKVENGSYVEAGDELTEGSVNPHDILKIKGLRGVQ
                     DYLLQEVQRVYRLQGVDINDKHIEVIVRQMLKKVRVDENGDTDFLPGSLVDRMEFEET
                     NKRMREQGLKEAEGKIVLLGITKASLATDSFLSAASFQETTRVLTDAAIKGKKDPLIG
                     LKENVIIGKLIPAGTGMYRYRKIEVASAE"
     gene            11348..11587
                     /locus_tag="PLG49_02795"
     CDS             11348..11587
                     /locus_tag="PLG49_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOQ16274.1"
                     /translation="MKRLKTRHIVVGAKQVTKLLERKQAEVVYIAKNAEDRVVRRILE
                     MAKEQGVELVMVESMKELGEACNVEVETATAAVLK"
     gene            11607..12020
                     /gene="rpsL"
                     /locus_tag="PLG49_02800"
     CDS             11607..12020
                     /gene="rpsL"
                     /locus_tag="PLG49_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOQ16275.1"
                     /translation="MPTFNQLVRKGRKSIKHKSSSPALQKGFNSLKKKTTNLPSPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLTNGIEVTAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGTRYHIIRGTLDTAGVADRKQGRSKYGAKRPKK"
     gene            12161..12631
                     /gene="rpsG"
                     /locus_tag="PLG49_02805"
     CDS             12161..12631
                     /gene="rpsG"
                     /locus_tag="PLG49_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOQ16276.1"
                     /translation="MPRKGHVPKREVLPDPLYNSKVVTKLINNIMLDGKRGIAQKIVY
                     GAFDRIAEKTGREALEVFEEALNNIMPVLEVKARRVGGATYQVPVEVRPERRQTLGLR
                     WLVLYARQRGEKTMVERLANEILDALNNTGGAVKKKEDTHKMAEANKAFAHYRW"
     gene            12731..14821
                     /gene="fusA"
                     /locus_tag="PLG49_02810"
     CDS             12731..14821
                     /gene="fusA"
                     /locus_tag="PLG49_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ16277.1"
                     /translation="MAGREFPLERTRNIGIMAHIDAGKTTLSERILFYTGRTYKIGET
                     HEGSATMDWMEQEQERGITITSAATTCFWKDYRINLIDTPGHVDFTVEVERSLRVLDS
                     AVGVFCAKGGVEPQSETVWRQADKYNVPRMAFVNKMDIMGADFYNVVDMIKERLHPNA
                     VPIQLPIGKEETFTGIIDLMKMKAYIYKDDLGKEIVEEEIPEDMKDKAEEYRMAMIEA
                     IAETDEELMMLYLEGEELTEEQLKKALRAATIKVEIIPVLCGSAYKNKGVQKLLDAVL
                     DYLPAPTDIPAIKGIIPDTGEEVERHSSDEEPFAALAFKIMSDPYVGKLAYFRVYSGI
                     INSGSYVLNSTKNKKERIGRILQMHANHRNEIDKVYSGDIAAAVGLKFTTTGDTLCDE
                     QHPVLLESMVFPEPVISVALEPKTKAGQEKMGIALSKLAEEDPTFKTYTNQETGQTII
                     AGMGELHLEIIVDRLLREFKVEANVGEPQVAYKETIQKAVDVEGKFVRQSGGRGQYGH
                     CKVKFEPLDPNGEKTYEFESQVVGGAIPKEYIQPINDGIQEAMQSGILAGYPVLGVKA
                     VVYDGSYHEVDSSEMAFKIAGSMAFKEAMKKGDPILLEPIEKVDVTVPEEYMGDVIGD
                     LNSRRGRIEGMEARNGLQIIHGFVPLSEMFGYATDLRSKTQGRGTYSMEFHHYEPVPK
                     NIQDKIVSGKGKAE"
     gene            14897..16090
                     /gene="tuf"
                     /locus_tag="PLG49_02815"
     CDS             14897..16090
                     /gene="tuf"
                     /locus_tag="PLG49_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOQ16278.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKTLHARYQLGE
                     EIPFDHIDKAPEEKERGITISTSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AIIVVAATDGPMAQTREHILLARQVGVPYIVVFMNKCDMVDDPELLDLVEMEIRDLLS
                     EYDFPGDEIPVIRGSALKALEDPSSEWGDKIIELFEAVDSYIPEPQREVDKPFLMPVE
                     DIFSITGRGTVATGRVERGVLKVQDEVEIVGLTDEPRKVVVTGVEMFHKMLDQAQAGD
                     NIGALLRGVQKDEIERGQVLAQPGTITPHTKFKAQVYVLKKEEGGRHTPFFTNYRPQF
                     YFRTTDVTGVIKLPDGVEMCMPGDNVEMTIELIAPIAMEKGLRFAIREGGKTVGAGSV
                     TEIIE"
     gene            16427..16738
                     /gene="rpsJ"
                     /locus_tag="PLG49_02820"
     CDS             16427..16738
                     /gene="rpsJ"
                     /locus_tag="PLG49_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOQ16279.1"
                     /translation="MANQKIRISLKAYDHKLIDQSAAKIIETAKKTGAKVSGPIPLPT
                     KKEVITILRAVHKYKDSREQFEIRTHKRLIDIVVPTPKTVDALMRLDLPAGVDIKVDV
                     K"
     gene            16852..17484
                     /gene="rplC"
                     /locus_tag="PLG49_02825"
     CDS             16852..17484
                     /gene="rplC"
                     /locus_tag="PLG49_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOQ16280.1"
                     /translation="MKKAILGKKIGMTQIFDENGKHVPVTVIEAGPCVVVQKKTTEND
                     GYNAIQVGFLDVKENRVNKPLKGHFEKAGVTPKRTLKEFRLDDISSYEIGSEIKADIF
                     APGDKVDVTGISKGKGYQGVIKRHNQGRGPMAHGSKYHRGVGAMAGASYPGRVLKGKK
                     LPGHMGAEKVTVQNLEVVRVYPEKNLILVKGAVPGPKKSIVMLKDSVKNA"
     gene            17512..18132
                     /gene="rplD"
                     /locus_tag="PLG49_02830"
     CDS             17512..18132
                     /gene="rplD"
                     /locus_tag="PLG49_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOQ16281.1"
                     /translation="MAKVAVYNMDGQQVDEIELKDEIFGVEVNEHIMHAAVVQYLANQ
                     RQGTQSAKTRAEVRGGGRKPWRQKGTGRARQGSIRAPQWTGGGVVFAPKPRDYSFKLN
                     KKVKRLALKSALTSKVQNNNLIVLDELKLDEIKTKNMKKVLDNLKVSKALIVTESDNK
                     NVILSARNLPNVKTAAVNTINVYDILKYESFVVSKDVLQTIQEVYA"
     gene            18132..18431
                     /gene="rplW"
                     /locus_tag="PLG49_02835"
     CDS             18132..18431
                     /gene="rplW"
                     /locus_tag="PLG49_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOQ16282.1"
                     /translation="MADLKYYDVILKPVITEKSMNLMAEKKYTFLVHPAATKNQIKEA
                     VEKMFEGVKVKKVNTMLQDGKTKRRGMTIGRTAKTKKAIVQLAEESKDIEFFEGM"
     gene            18485..19315
                     /gene="rplB"
                     /locus_tag="PLG49_02840"
     CDS             18485..19315
                     /gene="rplB"
                     /locus_tag="PLG49_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOQ16283.1"
                     /translation="MGIKTYKPYTPSRRHMTSLTFEEITKSTPEKSLVVSLKKNSGRN
                     NQGKITVRHRGGGAKIKYRIIDFKRNKDGIPARVAAIEYDPNRTANIALLHYADGEKR
                     YILAPKGLKVGDKIMSGETAEIRVGNALPMKNIPVGTTIHNIEMKPGKGGQLVRSAGA
                     EAQLLAKEGKYATVRLPSGETRLLPIECRATIGQVGNIEHELVTIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRPTPVTPWGKPTLGYKTRKKNKASNKYILHRRTK
                     "
     gene            19357..19641
                     /gene="rpsS"
                     /locus_tag="PLG49_02845"
     CDS             19357..19641
                     /gene="rpsS"
                     /locus_tag="PLG49_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOQ16284.1"
                     /translation="MARSLKKGPFADEHLLKKIEEMNKSGEKKVIKTWSRRSTIFPQM
                     VGHTIAVHDGRKHVPVYITEDMVGHKLGEFALTRTYRGHGKNAEKKSGAR"
     gene            19670..20056
                     /gene="rplV"
                     /locus_tag="PLG49_02850"
     CDS             19670..20056
                     /gene="rplV"
                     /locus_tag="PLG49_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOQ16285.1"
                     /translation="MAKGHRSQIKRERNQNKDTRPKAHLRYARVSASKAKIVLDTIKG
                     KNANEALAILAYTPRKAARIIEKLLKSAIANAENNHELDREKLFIEEAYANQGPKMKR
                     IRPRAQGRAYRIDKQTSHISIVLNER"
     gene            20071..20742
                     /gene="rpsC"
                     /locus_tag="PLG49_02855"
     CDS             20071..20742
                     /gene="rpsC"
                     /locus_tag="PLG49_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOQ16286.1"
                     /translation="MGQKVNPHGLRVGIIKDWSTKWYASKQEYADLLVEDYKLRDYIK
                     NKLYDAGISKIDIERAAERVKINIHTSKPGIVIGKGGSSIEQLRNELQKLTKHKVMVN
                     IIEIKKPEIDAQLVAENVALQLENRVSFRRAMKQVMARAMKNGAKGIKVACSGRLAGA
                     EIARTEHYHEGTIPLQTLRADIDYGFAEADTTYGKIGVKVWIYKGEILPQKAKKEGEI
                     NNVNA"
     gene            20729..21169
                     /gene="rplP"
                     /locus_tag="PLG49_02860"
     CDS             20729..21169
                     /gene="rplP"
                     /locus_tag="PLG49_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOQ16287.1"
                     /translation="MLMPKRVKHRKQFRGRMKGRATRGNKITYGEFGLVALEPGWITS
                     NQIEAARVAMTRYIRRGGKVWIKIFPDKPVTQKPAETRMGAGKGSPEYWVAVVKPDRV
                     MFEIAGVNQETAREALRLAMHKLPIKCKIVSRADQEMDGDSSEK"
     gene            21159..21362
                     /gene="rpmC"
                     /locus_tag="PLG49_02865"
     CDS             21159..21362
                     /gene="rpmC"
                     /locus_tag="PLG49_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOQ16288.1"
                     /translation="MKSKAYLDELRNKTLDELKSDLVSAKKELFNLRFQNATNQLSNT
                     ARIKEVRKNIARIQTVISERAKA"
     gene            21390..21647
                     /gene="rpsQ"
                     /locus_tag="PLG49_02870"
     CDS             21390..21647
                     /gene="rpsQ"
                     /locus_tag="PLG49_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOQ16289.1"
                     /translation="MGERKPRKTLIGKVVSDKMDKTIVVAVENNVKHPLYGKIIKRTY
                     KLKAHDENNECKIGDLVKVMETRPLSKEKRWRLVSIVEKAK"
     gene            21672..22040
                     /gene="rplN"
                     /locus_tag="PLG49_02875"
     CDS             21672..22040
                     /gene="rplN"
                     /locus_tag="PLG49_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOQ16290.1"
                     /translation="MIQQESRLKVADNTGAKELLCIRVLGGSKVRYANVGDIIVASVK
                     DATPGGVVKKGDVVKAVVVRTVKGVRRPDGSYIKFDENAAVIIKEDKNPRGTRIFGPV
                     ARELREKKFMKILSLAPEVL"
     gene            22054..22410
                     /gene="rplX"
                     /locus_tag="PLG49_02880"
     CDS             22054..22410
                     /gene="rplX"
                     /locus_tag="PLG49_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOQ16291.1"
                     /translation="MKKDKKEQKIKTRLKVGDKVRVISGECEGNEGKILKIDRKTGRV
                     IIEGVNMITKHEKTNAMGQGGIIKQEGPIHISNVMYLHNGKPTRLGVKVVEEERNGRM
                     KKVRYRVAKSTGEVID"
     gene            22431..22970
                     /gene="rplE"
                     /locus_tag="PLG49_02885"
     CDS             22431..22970
                     /gene="rplE"
                     /locus_tag="PLG49_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOQ16292.1"
                     /translation="MSRVRELYENEIVEQMMKKFGYKNKLEVPKLEKIVINMGVGEAK
                     DNPKALEAAVNDLTAISGQKPVITKAKKSVANFKIRTGMPIGCKVTLRGRRMYDFVDR
                     LISLALPRVRDFRGVNPDSFDGRGNYALGIIEQLIFPEIDFDKVDKVRGMDIIFVTTA
                     KTDEEARELLTLFGMPFRK"
     gene            22988..23173
                     /locus_tag="PLG49_02890"
     CDS             22988..23173
                     /locus_tag="PLG49_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOQ16293.1"
                     /translation="MAKKSMIVKQQRKPKFSTRAYNRCRICGRPHAYLRKFGVCRICF
                     RELAYKGQIPGVKKASW"
     gene            23193..23594
                     /gene="rpsH"
                     /locus_tag="PLG49_02895"
     CDS             23193..23594
                     /gene="rpsH"
                     /locus_tag="PLG49_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOQ16294.1"
                     /translation="MTMTDPIADMLTRIRNANIAKHDTVDIPASKMKQAISDILLNEG
                     YIKGYEIIEDGPKKTIRITLKYGKDKNEKVISGLKRISKPGLRVFVGKDELPKVLGGL
                     GTAIISTSKGVVTDKEARKLGVGGEVVAFIW"
     gene            23654..24190
                     /gene="rplF"
                     /locus_tag="PLG49_02900"
     CDS             23654..24190
                     /gene="rplF"
                     /locus_tag="PLG49_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOQ16295.1"
                     /translation="MSRIGKLPIAIPSGVEVKIGEGNLVTVKGPKGTLERKLSPEMLI
                     TIEDNQVIVKRPSDLKKHKALHGLTRTLVNNMVEGVTNGFTKELEINGVGYRAQKQGK
                     KLVLSLGYSHPVEMEDPEGIESTVEGNKIIVKGINKEKVGQYAAEIRAKRPPEPYKGK
                     GIKYVDEVIRRKEGKTGK"
     gene            24212..24580
                     /gene="rplR"
                     /locus_tag="PLG49_02905"
     CDS             24212..24580
                     /gene="rplR"
                     /locus_tag="PLG49_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOQ16296.1"
                     /translation="MIKKEPRSKIRVNKHLKVRKKIFGTSERPRLSVFRSDKHIYAQI
                     IDDTKGHTLVAASTVEKDIKSKIEKTNNVDAAKVVGEMIAKRALDKGVKQVVFDRGGY
                     IYHGKIKALADAAREAGLEF"
     gene            24597..25106
                     /gene="rpsE"
                     /locus_tag="PLG49_02910"
     CDS             24597..25106
                     /gene="rpsE"
                     /locus_tag="PLG49_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ16297.1"
                     /translation="MAKAKIDVNSLELKERVVTIKRVTKVVKGGRNFRFSALVVVGDE
                     NGHVGAGLGKAAEIPDAIRKAIEDAKKNIIYVPRDKNDSLPHEFIGEFGSSRVLLKPA
                     PEGTGVIAGGPVRAVLELAGVRNVRTKSLGSNNKRNVVNATMEGLKNIKDSSEVAKLR
                     DKTVEELLG"
     gene            25119..25301
                     /gene="rpmD"
                     /locus_tag="PLG49_02915"
     CDS             25119..25301
                     /gene="rpmD"
                     /locus_tag="PLG49_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOQ16298.1"
                     /translation="MADKLRITLVKSTIGAKPKHKEIVRSLGLNKLNKTVEQKDNPAI
                     RGMVEKVSHLVKVEEI"
     gene            25328..25768
                     /gene="rplO"
                     /locus_tag="PLG49_02920"
     CDS             25328..25768
                     /gene="rplO"
                     /locus_tag="PLG49_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ16299.1"
                     /translation="MNLSVLKPNEGSKHSRTRKGRGHASGLGKTSGRGHKGQKARSGG
                     GVRPGFEGGQMPLYRRLPKRGFTNINSKEIVAINVDRLNIFEDGTEVTVDTLKEAGII
                     KNPKDGVKILGNGDLNKKLTVKVNQFSKKAVEKIEAAGGKAEVI"
     gene            25769..27040
                     /gene="secY"
                     /locus_tag="PLG49_02925"
     CDS             25769..27040
                     /gene="secY"
                     /locus_tag="PLG49_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ16300.1"
                     /translation="MTEALKSVRNIWKIPDLRKKVIYTLLMLFVIRIGAHVPVPFVNA
                     AAVREVLEMQGTGPLKLINMISGGAFEDMTIFALGIVPYINASIIFQLLQIAIPKLEE
                     IAKEGEEGRKRIAKYTRYLTIALAALQAWGTTFALRNQNILIYSNLWTWIVVIVTFVA
                     GTTFLVWVGEQITEKGVGNGISLIIFINIVSRLPLAITTLFSYKDKYISIILLIVIFV
                     LMIAFVVLVQDGERRIPVQYAKRVAGRRIYGGQSTHIPLKANLGGVMPIIFAMSILQF
                     PIMMTNFFTSNPTGLWGKIITYINWTHPVGAILYAVLIFGFSFFYSTVAFNPVDVADN
                     MKKNGGFIPGIRPGNPTAEYLRKTLNNITVVGALFLTIIALLPIVLQWIFKMSIGFGG
                     TSIIIVVGVALETVQQIEAQMLMRHYKGFLS"
     gene            27063..27713
                     /locus_tag="PLG49_02930"
     CDS             27063..27713
                     /locus_tag="PLG49_02930"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOQ16301.1"
                     /translation="MRLIMLGAPGAGKGTHALLLSKEYNIPQISTGDILRSHIKNETD
                     LGKKAKKYMDKGLLVPDELVVEIVKKRIQEEDCKEGFILDGFPRTIPQAEALDKALEE
                     LAIALDKVVNIDVPDEVIIKRMAGRRVCSNCGASYHIENKKPLKENICDECGSELKQR
                     EDDKEETVRKRLQVYEEQTKPLIEYYEKRGILLTIEGDGSVEEIGNKIKNALEVSK"
     gene            27713..28462
                     /gene="map"
                     /locus_tag="PLG49_02935"
     CDS             27713..28462
                     /gene="map"
                     /locus_tag="PLG49_02935"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ16302.1"
                     /translation="MGITIKTDEEIRKIREAGRILAKTQELLASKIKAGITTMELDQI
                     AEEFIRSQGATPSFKGLYGFPASICTSINEEVVHGIPSVKRMLKEGDIISIDVGVCLD
                     GYHSDGAKTYAIGEVAEETKRLIEVTKQSFYEGIRFARPGNHLYEISSAIQKYVEANG
                     FSVVRDLTGHGVGRKLHEEPQIPNFAVPGRGPKLVKGMVLAIEPMVNMGSYQVRILAD
                     NSVVTRDGSLSAHYEHTIAITDGEPEILTTL"
     gene            28486..28779
                     /locus_tag="PLG49_02940"
     CDS             28486..28779
                     /locus_tag="PLG49_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOQ16303.1"
                     /translation="MNEYSIGQVVFSKSGRDKGRAFIVIQIENEYLYLVDGDLRKLSK
                     PKKKKNKHVQKTNYIIESIKKKIEDGVELNDAEIRKSLEPYSPSSRNAINEEA"
     gene            28784..29002
                     /gene="infA"
                     /locus_tag="PLG49_02945"
     CDS             28784..29002
                     /gene="infA"
                     /locus_tag="PLG49_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOQ16304.1"
                     /translation="MAKSDVIEVEGTVLEKLPNAMFQVELENGHKILAHISGKLRMNF
                     IRILPGDKVTVELSPYDLTKGRIVWRSK"
     gene            29030..29143
                     /gene="rpmJ"
                     /locus_tag="PLG49_02950"
     CDS             29030..29143
                     /gene="rpmJ"
                     /locus_tag="PLG49_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOQ16305.1"
                     /translation="MKVRPSVKPICEKCKIIKRKGRIRVICENPKHKQKQG"
     gene            29359..29727
                     /gene="rpsM"
                     /locus_tag="PLG49_02955"
     CDS             29359..29727
                     /gene="rpsM"
                     /locus_tag="PLG49_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOQ16306.1"
                     /translation="MARIAGIDLPRDKRVEIGLTYIYGIGRASSNRILKEAGVNPDTR
                     VRDLTDDEVSRIREIIEKTQVVEGDLRREVALNIKRLMEIGCYRGIRHRRGLPVRGQN
                     TKNNARTRKGPRRTVANKKK"
     gene            29751..30152
                     /gene="rpsK"
                     /locus_tag="PLG49_02960"
     CDS             29751..30152
                     /gene="rpsK"
                     /locus_tag="PLG49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOQ16307.1"
                     /translation="MAKKVAKKATSRRRREKKNVDRGQAHIQSTFNNTIVTLTDMAGN
                     ALSWASAGQLGFKGSKKSTPYAAQMAADAAARAAMEYGLKSVEVLVKGPGSGREAAIR
                     ALQAAGLEVTMIKDVTPVPHNGCRPPKRRRV"
     gene            30174..30800
                     /gene="rpsD"
                     /locus_tag="PLG49_02965"
     CDS             30174..30800
                     /gene="rpsD"
                     /locus_tag="PLG49_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOQ16308.1"
                     /translation="MARYIGPVCRLCRREGQKLYLKGEKCFSQKCPVVRRPYAPGQHG
                     QVNKKLSEYGLQLREKQKAKRIYGILETQFANYFEEADRIPGITGENLLRILEMRLDN
                     VVYRLGLGRSRTEARQVVRHNHILVNGKKVNIPSYQVKVGDVIEVKEKSKGLQRFKDI
                     TDTTASRIVPEWLEADMDNLKGKVVALPERDQIDIDIQETLIVELYSK"
     gene            30862..31821
                     /locus_tag="PLG49_02970"
     CDS             30862..31821
                     /locus_tag="PLG49_02970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOQ16309.1"
                     /translation="MFEFEKPRIEIAEMSEDGKYGRFVLEPLERGYGTTLGNCLRRVL
                     LSSLPGVAVSNVKIEGVLHEFSTIPGVKEDVPEIILNLKGLALKNNSDSNEPKIAYIE
                     AEGEGEVTAADIKVDSDIEIINPDLHIATLSGGPDSRLFMEMTITRGRGYVPAEKNKM
                     INHAIGVIPVDSLYTPVSRVNFTVENTRVGQITDYDKLTLELWTNGTIKSDEAVSLAA
                     KILSEHLNLFIDLSDNAKNAEIMVEKEEDQKEKVLEMSVEELDLSVRSYNCLKRAGIN
                     TVEELISRTEEEMMKVRNLGKKSLEEVLNKLAELGLSLKPSDD"
     gene            31856..32386
                     /locus_tag="PLG49_02975"
     CDS             31856..32386
                     /locus_tag="PLG49_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOQ16310.1"
                     /translation="MAGHRKLGRPTSQRKALLRNQVTNLLYHGRIKTTEAKAKEVRKL
                     AEKLITLAIKEKDNYTEVTVKAKVARKDAKGKRVKEVVNGKKVDVFDEVEKTIKKDNP
                     SRLHARRRMLQILVPVTETSKIKKKQNTKKVDMVEKLFSDIAPKYADRNGGYTRIIKL
                     GPRKGDGAEEVIIELV"
     gene            32537..33400
                     /locus_tag="PLG49_02980"
     CDS             32537..33400
                     /locus_tag="PLG49_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOQ16311.1"
                     /translation="MKEIVKSRGLIYEYYTHNERGEIEATNRALDRLDLDIKQGEFVV
                     ILGHNGSGKSTLAKHMNAILHPTGGTLLIKGMDTKDEENLWDIRQTAGMVFQNPDNQI
                     IAAIVEEDVAFGPENLGVEPGEIRKRVDEAVAAVQMTEYMTHSPNLLSGGQKQRIAIA
                     GVLAMKPECIILDEPTAMLDPLGRKEVLETVYKLNKEENITIIHITHYMEEAINADRV
                     IVMDHGQIVMDGKPKEVFSQVDKLKSLGLDVPPATELAYYMQKEGIDISTEIISIEEM
                     VDKIWQLYLTE"
     gene            33376..34230
                     /locus_tag="PLG49_02985"
     CDS             33376..34230
                     /locus_tag="PLG49_02985"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOQ16312.1"
                     /translation="MAIIFNGVSHVYGEGSPFKRVAINNINLHIKKGEFIGIIGHTGS
                     GKSTLIQHFNGLLKPTEGEVIILGKNLNDKELSLKEIRQKVGLVFQYPEHQLFETTIF
                     KDVAFGPENMGLSKEEVDKRVREALEMVGIGEDMYDKSPFDLSGGQKRRVAIAGVLAM
                     KPEILILDEPTAGLDPRGRDEILGQIQKLHKSLGITVILVSHSMEDIAKLVDRIIVMY
                     KGRIALDGSPAKVFKNDKELEKMGLAAPQIMYIMKALRKKGLEVPVDVLTVEEGAKAI
                     AALLKSQK"
     gene            34239..35039
                     /locus_tag="PLG49_02990"
     CDS             34239..35039
                     /locus_tag="PLG49_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOQ16313.1"
                     /translation="MIRDITIGQYFPAESWIHKLDPRVKIMSTLIFIIALFVANRYIG
                     YLFTFIFMATVIYLSKVPVKYMLKGIKAILVIIILTVALNIFMTPGEKVLWSYKSINI
                     TAEGLYIAGIMALRLIFLIIGSSVLTLTTSPIQLTDGLEKLLNPFKKIGIPAHEIAMM
                     MTIALRFIPILLEETDKIMKAQMARGADFESGGIIKRAKSLIPLLVPLFISAFRRADE
                     LAMAMEARCYRGGENRTRMKQLKIKKQDYVAMVSIGLFMGIMIVSRFI"
     gene            35080..35814
                     /gene="truA"
                     /locus_tag="PLG49_02995"
     CDS             35080..35814
                     /gene="truA"
                     /locus_tag="PLG49_02995"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ16314.1"
                     /translation="MKHILLTIAYDGSNYNGWQRQSNGIGIQEKVEEACFKIFGQEIS
                     VTGAGRTDAGVHALGQRGLIKVETSIPTDKILYALNSVLPKDIVIRAAREVPENFHPR
                     YDAREKTYRYLILNDKFPIPQYRNISTFISAQLNVEEMDKGAKYFIGNHDFAAFSSAG
                     SSVKTTERTIFDALVYREDPFVIFEVRGNGFLYNMVRIMAGTLIEIGIGKKKASDVKD
                     IILSRDRSKAGITAPAQGLTLVEIKY"
     gene            35970..36407
                     /gene="rplM"
                     /locus_tag="PLG49_03000"
     CDS             35970..36407
                     /gene="rplM"
                     /locus_tag="PLG49_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ16315.1"
                     /translation="MRTTYMAKAEAVERKWYVVDAKDMTLGRLSSEVAKVLRGKHKPT
                     YTPHIDTGDFVIVINAKDIKVTGKKLDQKLYRHYTGYAGGLKEISLRDLLEKKPEKVI
                     YEAVRGMLPKNRLGRKMLKKLRVYSGPEHKHEAQKPEVLEIKF"
     gene            36429..36821
                     /gene="rpsI"
                     /locus_tag="PLG49_03005"
     CDS             36429..36821
                     /gene="rpsI"
                     /locus_tag="PLG49_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ16316.1"
                     /translation="MAEARYYGTGRRKKSIARVYLVPGNGNFKINKRDIEEYFGLETL
                     KVIAKQPLVLTNTVDKFDVLVNVYGGGYTGQAGAIRHGIARALLQADPELRPVLKKAG
                     FLTRDPRMKERKKYGLKGARRAPQFSKR"
BASE COUNT        13585 a         5511 c         8060 g         9724 t
ORIGIN      
        1 aaatttttta tttatatatt gactaattag ccactccatg ttatattagt taagtggcga
       61 aaggtctttt ttttatgtct aaaaataata gactgaatta gcatagtaag tggaggtgga
      121 agttgtgaga gtaaaaatta cattagcatg tacagaatgc aaacaaagaa attataatac
      181 aactaaagaa aaaagaaagc atcctgacag gatggaaatt aaaaagtact gcagattttg
      241 cagaactcat accttgcaca aggaaaccaa gtagtaaaat aaaattcctg taaggaagtg
      301 aatttatgaa agaacgcttt ttacaagtaa aaggtgaatt taaaaaaatc atatggccca
      361 ataaaaaaga tttagttaaa caaactacaa cggttattgt gacttcttta ctgacagctg
      421 gaattgtctt tgtaattgat tttgcataca gcagagggct tgacaaatta atggaaatat
      481 tgaagtaaga ggatgatata atgtctgaag atgcaaaatg gtatgtggtc catacctatt
      541 ccggatatga gaacaaggtg aaagccaata ttgaaaaggt tattgaaaac aggaatatgc
      601 aggacctgat ttgtgaagtt gttgttcctc ttcaagacac ggttgaaatt aaaaatggaa
      661 agcgaaagac agtacaaaag aaaatatttc cagggtatgt attagtaaag atgcatatga
      721 cagatgaaac ctggtatgtt gtcagaaaca caagaggagt aaccggattt gtagggcctg
      781 gttcaaaacc cgttcctctt acagaagaag aaataagagg tatgggcatt gatcttcctc
      841 aggcaaaaat tgattttgaa gttggagata ctgtaaaagt tattacagga ccttttgaaa
      901 actctgttgg tgtaataaat catattaatg agaacaagag aaccgttaca gttagattat
      961 ctatgtttgg ccgggaaaca ccggttgaac ttgatttttc tcaaatacaa aagctataat
     1021 aattaactag tgcatgggaa accatgacca gtgggaggga aattcccgca aataccacat
     1081 tattaggagg tgcgctttaa tggcgaaaaa agttattgga atgattaaat tgcagattcc
     1141 tgcaggaaag gcaacaccag caccacccgt tggaccagct ttaggacaac atggtgtaaa
     1201 tattatgggt ttttgtaaag aattcaatga aagaacagca aaacaagcgg gatacgttat
     1261 ccctgttgta attactgttt atcaggacag aagttttaca tttattacaa aaactcctcc
     1321 ggcagcggaa ttactgaaaa aagcatgcaa aattgagttt ggttctggag cacctaacaa
     1381 agaaaaagtt gcaaccatat ccagagaaga agttaagaaa attgctgaat taaaaatgcc
     1441 ggatttaaat gctggaagca ttgaagctgc tatgagcatg attgaaggaa ctgcaagaag
     1501 catgggtata gttgttaaag actaataaaa acatggataa atcttatgaa ttaaaaaagt
     1561 gggagggcat ctgtccccga taataccaca aggaggtaat gaaatgaaaa gaggaaagaa
     1621 atatattgaa gctgcaaagc ttgttgaccg tactacatta tatgaaccaa aagaagcttt
     1681 cgatttagtt caaaaaattg ctactgcaaa atttgatgaa actgtagaag ctcatatccg
     1741 attaggcgtt gactcaagac atgctgacca acaagttaga ggcgccgttg tgcttcccca
     1801 tggaactggt aagagcgtaa aagttcttgt ttttgcaaaa ggggacaagt taaaagaggc
     1861 agaagatgca ggcgcagatt atgttggagg cgaagagctt attccaaaaa ttcaaaatga
     1921 aggatggctt gattttgatg tagttgttgc tacacctgac atgatggctg ttgtaggacg
     1981 tatcggtcgt gttttaggtc caaaaggatt aatgcctaat ccaaaagcag gaacagttac
     2041 gatggatgta gcaaaagcgg ttaaagatat taaagcaggt aagatagaat accgtttaga
     2101 taaaacaaat attattcacg tgccaattgg taaagtatcc tttggtacag aaaaattact
     2161 cgacaacttc caaacactta tgagtgcaat tataaaagct aaaccagcag cggcaaaagg
     2221 tcaatacctt aagagtattt ctgtatcttc cactatggga ccaggaatca aaatcaatcc
     2281 tttaaaagtt gaagcataga ttttgcgatg atttgtcagt aaagtataag aaaaatatat
     2341 tgacaagtca gatgggaaat aatataattg tttatgttgt aaaataaaac tgaagattgt
     2401 aaaaccgcag acagtaggtg ccctgagggc gtaacgagag atctacctac cgaggttttg
     2461 attattgtga ttgaatgata atttatacct ctttgtctgc ggatgcagat gaagaggttt
     2521 tatttttgat aaatccttaa ggaggtggaa gtaagtggcg aagatagaaa agaagcagga
     2581 aattgtcaat gaaattaagg aaaaattaag caaatctgca tccgttgttc tggttgatta
     2641 tagaggactg acggttgaac aagatactga actcagaaga aaattccgtg aagcaggtgt
     2701 tgattataag gtttataaaa acaccatgat gaactttgcg atccaggata cggaatttga
     2761 accacttaaa gattatttag aaggacctag tgctgtagca tttagctatg atgatccaac
     2821 gaatggtccc agaataatag atgaagttgc ttcaaagtat aatgctttag aatttaaagc
     2881 aggtatcgta gaaggtagat tttacgatac agcaggaatt gcaaaagtgg caaaaatacc
     2941 accaaaagat caattgcttt ccaaattact tggaagcttc aattcaccga ttgcatcctt
     3001 tgctcgtgta attaaagcaa ttgcagataa aaaagcagaa ggtgcagaag cagtaacaga
     3061 ataaaaaaaa taataatatt ttcggaggtg tcttaaatgg caaaattaac agttcaagaa
     3121 attttagatg cgattgatga acttactgtt ttagaattaa atgaattagt agaaaaatgt
     3181 gaagaaaaat atggcgtatc agctgctgca ggcgtagtag ttgctgctgc cggagctgct
     3241 gcaggcggag ctgctgcaga agaaaagaca gaatttgatg tagaattaac tgcattaaat
     3301 gatgcaagtg caaaagttaa agtaattaaa gtagttagag aattaacagg acttggatta
     3361 aaagaagcta aagacttagt agaagctgtt ccaaaagtag taaaagaagg cgtatcaaaa
     3421 gaagaagcag aagaagtaaa agcaaaatta gaagaagcag gcgcaaaagt tacaataaaa
     3481 taaccaaaat aaaatttata aaattgaaaa aaataaaatc tcccatggga gattttattt
     3541 tttcattata atacaaaatg accttgacag gtcattctaa ttgtgctaag attttaaaat
     3601 gcgtcaatat gtatatttaa aattttgatt caatttatta taacatattc acatagaatt
     3661 gacaatatgc attaagaatt ttattgttaa ttactatgtt catggtaata aaatgcaaat
     3721 ttgcatttaa gaagtataaa taattcaagg ggtgagcatt tacatgaaaa aaaatagaat
     3781 tcatcccgtg cctgccggga aaggagttcg catgagctat gccagaatgg aagaagttct
     3841 ggaaatgcca aacttaattg aggttcaaaa aaactcttat aagtggttct tggaggaagg
     3901 gttaaaagag gttttccagg atatctcccc tatcactgat tacagtggca atttaatgct
     3961 tgaatttgtt gatttttctt tggacgaaaa ccccaagtat tctattgaag aatgtaagga
     4021 aagagacgct acatatgcaa cagccttaaa ggcaaaagta cgtttgtata ataaagagac
     4081 cgaggaaatt aaagagcagg aaatttttat gggagatttc ccactgatga cagaaacagg
     4141 aacctttatt attaatggtg cggaaagagt tattgtaagc cagctggtta gatcaccggg
     4201 tatttatttt gatgttgatt atgataaagt aggtaaaaag ctttttactg caacggttat
     4261 tcctaacaga ggtgcctggc ttgaatatga aacggattct aatgacgtat tttatgtaag
     4321 agtggaccgt acaagaaaag ttcctattac tgccttaata cgggctttag atataggaag
     4381 cgatgaaaga attatagaat tatttggaga agagccaaaa atattatcaa cgcttgaaaa
     4441 agataataca aaaactcatg aggaaggatt aattgagata tataaaagaa tccgtccggg
     4501 agagcctccg acggtagaca gtgctaaaac tttgctttac aacatgtttt ttgatcccaa
     4561 acgatatgat ttagcaaagg ttggccgtta taaatttaat aaaaaattag ctttcagaaa
     4621 tagaatccgt aactttatcc tggcagaaag tgttgtagac agtacgacag gagaagttat
     4681 cgcagaagca ggcgaaagaa ttactccgga attagcggat gcaattcaag atttgggtat
     4741 tcaatctgtt ttaataaaag tgcctcatca tgataaacct gtaaaaattc ttagtaactt
     4801 aactgtaaat attgaaaagc acatagatat agatgcagag gaattaggca ttaaggacag
     4861 ggtatattat ccggttctga aggaattaat ggaaaaatat gaggacctgg aagagctgaa
     4921 gcaagccatc aaagagaata ttcaaagact aattccaaag catattacaa aagaagatat
     4981 aatggcgtct attaattatg gaattcattt ggaatatggt ataggtaatg tagatgatat
     5041 cgaccacctt ggaaatagaa gaatccgtgc tgtaggggag ttattccaaa atcaattccg
     5101 tataggttta gccagaatgg aaagagttat ccgtgaaaga atgactattc aagatttgga
     5161 agcagtaacg ccacaaaatc ttatcaatat aagacctgta tctgctgcaa ttaaggaatt
     5221 ctttggaagc tctcaattat ctcaattcat ggatcaaacc aatccgttag ctgaattaac
     5281 tcataaacga agattatcag ctcttggacc aggtggtctt tcaagagaaa gagcaggttt
     5341 tgaagtccgt gatgttcact attcccatta tggaagaatg tgtcctattg aaactccaga
     5401 aggacctaat atcggattga tcaactcctt agcaacttat gcaagaatta atgagtatgg
     5461 ctttattgag gcgccatacc gtattgtaga tcattctaca ggaattccta ttgtaaccga
     5521 taaagtggtt tatttgactg ctgatgaaga agaaaactat acagttgcag aagcaaatac
     5581 gcctattgat gaaaaaggct cctttattaa taagaaagtt acgggtagac ataaaagaga
     5641 aaccgttgaa gttgacccaa gcaagataga tttaatggat gtatctccta aacaattggt
     5701 ttctgttgca acagctatga ttcctttcct tgaaaatgac gatgctaacc gtgcactcat
     5761 gggatctaac atgcagcgtc aggcggtgcc tttattggtt acggaagcac caatcattgg
     5821 tacaggaatg gagtacagag ctgcaagaga ttcaggtgtt acggttattg ctaaaaaagc
     5881 aggtatcgtt gaaaaagtta cggcaacgga aattgttatt aagtctgatg atggagaacg
     5941 tcaaacatat aaattacaaa aattcgtaag aagtaaccag ggaacgtgta ttaatcaaag
     6001 ggctattgtt gataagggtc aaagaattga agctggagaa attatagcag acggcccttc
     6061 cacagatcat ggagaaattg cattaggtaa aaatgtattg gttggattta tgacctggga
     6121 aggttataac tacgaagatg ctgtcttgct tagtgaaaga ttagttcagg aagatgttta
     6181 tacttctatt catatagaag aatatgaagc agaagccaga gatacaaaat tagggccaga
     6241 agaaattaca agagatattc caaacgtagg agaagatgcc ttaaaagatt tggatgaaag
     6301 aggaatcatc cgcattggtg ctgaagtgcg ttcaggggat atcctggttg gaaaagtaac
     6361 gcccaaaggt gagacagagt taacagcaga agaaagactc cttagagcaa tctttggaga
     6421 aaaggcaaga gaagtaaggg atacctctct tcgagttcct cacggagaat caggaattgt
     6481 tgtcgatgtt aaaatcttta caagagaaaa tggtgatgaa ctttctccag gggtaaatca
     6541 acttgttcga tgttatattg ctcaaaagag aaagatttct gtgggagata aaatggcagg
     6601 tcgtcatggg aataaagggg ttatctccag aattttgcca gtagaagaca tgccttattt
     6661 acccaatggt cgtccattag acatcgtgct taatcctctt ggtgtgccat cacgtatgaa
     6721 tatcggtcag gtcttagaag tacacttagg actggcagcc aaaattttag gttggaaaat
     6781 tgctacaccg gtttttgacg gagcaaatga aatagatatt atggatacgc ttgatctggc
     6841 taatgattat gtaaatatgt cctgggaaga ttttgagaaa aaatggggtg aaacacttca
     6901 cccagaagca atgaaaaaac tggaagaaaa tttagaccag cgtgaagagt ggaagggagt
     6961 acctatcaat agaacaggta agattccttt aagagatgga cgaacagggg aatattttga
     7021 taatcctgtt actgtaggat atatgcatta tctgaaatta gcacacttag ttgatgataa
     7081 gattcacgca cgttcaacag gaccttatgc aaccattacc caacaacctc tgggaggaaa
     7141 ggcacaattt ggcggacaaa gatttgggga aatggaagta tgggctcttg aggcatatgg
     7201 tgcagcatac accctgcaag aaatattgac cattaaatca gacgacgttg taggccgcgt
     7261 gaaaacttat gaagctatcg tgaagggcga aaacattccc gaaccgggag taccagaatc
     7321 ctttaaagtc ctcttaaaag aattacaatc tctttgtctg gatgtgaaag ttttattaga
     7381 tgacgaaaca gaattagaaa taaaagaatc ggttgatgac attgatgaat taacggttaa
     7441 tatagaagga acagaagacg gacaagatta tctggttgga catagcgcga aagcgggtca
     7501 tctgatagat gatgatattg aagacgaaga tattgaagac gatgaagata ttagtgaaga
     7561 catcgatgac gacttttttg aaccatctga agaagaggac gaaatggacg ttttagattt
     7621 atttgacagt gatgatgata tagattttga aacggaatag gaaaggagag gaatccatgc
     7681 ctacagtaaa tatggatcaa tccgttttct ttgatgcatt aaaaatctca ctggcttctc
     7741 cggataaaat cagagaatgg tccaggggag aagtaaagaa accagaaacc attaattacc
     7801 gtaccctaaa gcctgaaaga gacggtttgt tttgcgaaag aatttttggt cccagtaaag
     7861 actgggaatg tcattgtggt aagtacaaac gcattcgtta taaaggtgta gtctgtgacc
     7921 gctgtggtgt tgaagttacc aagtctaagg taagaagaga aagaatgggt catatcgaat
     7981 tggcagctcc ggtatctcat atatggtatt ttaaagggat accaagccgt atgggcctta
     8041 ttcttgacat gtcccccaga gccttagaaa aagttttata ttttgcatcc tatattgtat
     8101 tagaaccagg ggacacaggt ttacagtata agcaactttt aacagaaaaa gaatacaggg
     8161 atgcatgtga gaaatacgga gcaaatgcat ttcgtgccgg aatgggagca gaagcaataa
     8221 aagaactttt atgtgctatt gatttagaaa aagaatcaca agaactaaag aaggctttaa
     8281 aagaaacgac tggtcaaaag agagtcagaa tcatcaaaag gttagaagtt attgaagcat
     8341 ttagaaaatc cgggaacaga ccagaatgga tgattctgga tgtgattccc gtattacctc
     8401 cggatttacg tccaatgatt caattagatg ggggacgttt tgcaacatct gatttgaatg
     8461 atttatacag aagagtgata aacagaaata accgcttaaa gagactcctt gacttaggag
     8521 cacccaatat cattgtacgt aacgaaaaga gaatgttaca ggaagcagta gatgcgctta
     8581 ttgataacgg cagaagaggc cgtcctgtaa cgggacctgg taacagagct ttaaaatcac
     8641 tttctgatat gctaaaaggt aagcaaggac gtttcagaca aaacttattg ggtaaacgtg
     8701 ttgactattc aggtcgttct gttatcgttg taggtcccag tttaaagatt tatcaatgtg
     8761 ggcttccaaa agaaatggcc atcgagttgt ttaagccatt tgtaatgaaa aaacttgttg
     8821 aagacggatt ggctcataat ataaagtctg ctaaaagaat ggttgaaaga cttcagtcag
     8881 aagtatggga tgttttagaa gaggttatta aagaacatcc tgtgatgtta aaccgtgcac
     8941 ctacattgca ccgtttggga attcaagctt ttgaaccagt gttagtagaa ggccgtgcca
     9001 ttaaacttca tccacttgta tgtacggctt ataatgcaga ctttgacggg gaccaaatgg
     9061 cggttcacgt acccctttcc gttgaagctc aggcagaatg cagattctta cttctttctc
     9121 caaacaacct tttaaaacct tcggatggag caccagtaac tgtaccttct caggatatgg
     9181 tattgggaac atattattta actcttgaga aggatggaga aaaaggtgaa ggtagtatct
     9241 tcaaagacga aaatgaagcg ataatggctt attacaataa atatatcagt cttcatgcca
     9301 agataaaagt tcgtagaaca aaagaaatca atggagaaaa gaagtcagct atagtcaata
     9361 caactgtagg ccgtattata ttcaatgaag caattccaca ggatatcggc tttgtagaca
     9421 gaactaaaga agaaaactta tttaaatatg aaatagactt tttagtagat aaaaaggctt
     9481 taggtaaaat tattgacaga tgtattaaca aatatggtgt taccaataca gcagttgtat
     9541 tggacaaaat taaagattta ggttttaaat attcaacaat tggcgcgatt accgtatctg
     9601 tatctgatat ggaaatccca agagaaaaac aagatatatt aaacgaagcc gaaacgacag
     9661 tcgatgaagt gactaagctg tacagacgcg gcttaatgac cgatgaggaa agatatcaaa
     9721 aagttattga aacctggaat aaagccaacg aaaaaattac cgatgccttg ctcaagagct
     9781 taggtaagta caacaatatt tatatgatgg cacattccgg ggcccgtggt tctatcaatc
     9841 agatcaaaca gttggcgggt atgagaggac ttatggccag tgcaacagga aaaattattg
     9901 aattgccgat taaggcaaat ttccgtgaag gcctgtctgt tctggaatac ttcatttctg
     9961 ctaacggtgc acgaaaaggg cttgcagaca ctgctttacg tacagcagac tcaggatatc
    10021 tgacaagacg tctggttgac gtagctcagg atgttattat ccgtgaagtt gactgttgca
    10081 gtaatgaaga agaagtacca ggaatgtggg ttaaagcctt taaagatggc gaagaagtga
    10141 ttgaatcctt acaagacaga atccgtggca gatggttgtt agaagacgtg attcatccgg
    10201 aaacaggaga agtattggct aaggccaata ccatgattac tccggatcag gcagaagcaa
    10261 ttgaaaaagc gggtgttaaa aaagtttata tcagaactgt attaacttgc cgctcaaaga
    10321 ttggtgtatg ttctaaatgt tatggcgcaa acatggcatc cggccgcctt gtaagagtgg
    10381 gagaatccgt agggatcatc gctgctcaat ccattggtga acctggaaca cagcttacaa
    10441 tgcgtacatt ccatacagga ggaattgcgg gagatgacat tacccaaggt cttccacgtg
    10501 tagaagaatt gtttgaagca agaaagccaa aaggacttgc tattattgca gaatttgccg
    10561 gtaaggttca gataaacgat acaaagaaaa aacgtgagat tgttataacg aacgaagaag
    10621 cgggagaatc taaatcttat cttgtacctt atggctcaag aataaaggtt gaaaatggaa
    10681 gttatgtaga agcaggagat gaactcacag aaggtagtgt gaatcctcat gatatcctta
    10741 aaattaaagg actaagaggc gtacaggatt atctgctgca agaagttcaa agagtttacc
    10801 gcctgcaagg tgttgacatt aatgacaagc acatagaagt tatcgttcgt caaatgctta
    10861 agaaagtcag agttgacgaa aatggggata cagacttcct tcctggaagc ctagtggata
    10921 gaatggaatt cgaagaaacc aataaacgaa tgagagaaca aggtttaaaa gaagcagaag
    10981 gcaaaatagt ccttttagga atcacaaagg cttctttagc aacagactct ttcttatctg
    11041 ctgcttcatt ccaggaaaca accagagtat taacggatgc agcgataaaa ggaaagaaag
    11101 atccgttgat tggtcttaaa gaaaacgtaa tcattggtaa actgatacct gctggtacag
    11161 gaatgtacag ataccgaaaa attgaagtag cttccgcaga gtaaagaaag tttttagggg
    11221 catgaattca acacatgccc ctaaaaataa agccttgaca tttttatggc taagtgatac
    11281 aattcttcag tgtgtgttgt atgatgggaa ataagcataa aaatgaacct tttagagggg
    11341 gatttatgtg aaaagattaa aaacacgaca catcgtagta ggtgcaaagc aagtaacgaa
    11401 attattggaa agaaaacaag cagaagttgt ttatattgct aaaaatgcag aagacagagt
    11461 tgtacgcaga attttagaaa tggctaaaga acaaggcgta gaacttgtca tggttgaaag
    11521 catgaaagaa ttaggagaag cttgcaatgt agaagtagaa actgcaacag cagcagttct
    11581 taaataataa aaggaggtgg aaaaggatgc ctacgtttaa ccagttagtg cgaaaaggaa
    11641 gaaagtcaat caaacataag tcatcatcac cggcacttca aaaaggattt aattccttaa
    11701 agaaaaagac aactaatctt ccatcccctc aaaaaagagg tgtttgcaca gctgttaaaa
    11761 cagctacacc taagaagcca aactcagcgc tcagaaaaat cgcgagggtt cgtcttacta
    11821 atggaataga agtaacagca tatattccag gagaaggaca caaccttcag gagcacagtg
    11881 tggttcttat aagaggtgga agagttaagg acctcccagg tacaagatat cacatcattc
    11941 gtggtacatt agatactgcg ggggttgctg acagaaaaca aggccgctct aaatatggag
    12001 caaaaagacc aaagaagtaa aagtaaaatg agtgcccagg cggatacaga tataatcctg
    12061 ctgtatgcag tttaatatag atgtagcgtt tctggagcac gtgcacacaa ttaagtgtga
    12121 gtaccgatga tataatatta tcgttaagga gggaagaatc gtgccacgta aaggacatgt
    12181 gccaaaaaga gaagtattac ccgatccgtt atataatagc aaggttgtaa caaaattaat
    12241 taacaacatc atgcttgatg gaaaaagggg tattgctcaa aaaatagtat atggtgcatt
    12301 tgacagaatt gcagaaaaaa caggcagaga agctttagaa gtgtttgaag aagctttaaa
    12361 taacattatg cctgttttag aagtaaaagc tcgccgtgtg ggtggagcaa cctatcaagt
    12421 gcccgtagaa gtaagacctg aaagaagaca aactttaggc ttacgctggt tagtattata
    12481 cgcaagacaa cgtggagaaa agaccatggt agaacgttta gcaaatgaaa tattagacgc
    12541 tttaaataat actggcggag ctgtaaagaa aaaagaagat acacacaaaa tggcagaagc
    12601 caacaaagca tttgcacatt acagatggta atcattatag atgcttatac catcggctat
    12661 gggcaagatg cgtgagttag acttgagtta ataaatagat tagtggaaac accaaaggag
    12721 gagtataact ttggcaggaa gagaatttcc cctcgagagg accagaaata ttggaattat
    12781 ggcccatatt gacgcgggga aaacaacatt atcagaaaga attttatttt acactggtcg
    12841 tacctataaa ataggagaaa cccatgaagg gtctgcgacc atggactgga tggaacaaga
    12901 acaagaaaga ggaattacca ttacatccgc tgcgacaaca tgtttttgga aagattatcg
    12961 cattaattta atagacacac cagggcatgt tgactttaca gtagaagtag aacgctcact
    13021 ccgtgtatta gacagtgcgg ttggtgtatt ttgtgcaaaa ggtggcgttg aaccccaatc
    13081 tgaaacagta tggcgtcagg cggataaata taatgttccc cgtatggctt ttgtgaataa
    13141 aatggacatc atgggagcag atttttacaa tgtagtggat atgataaaag aaaggctaca
    13201 tcccaatgct gttccaatcc aattgccaat tggcaaagaa gaaactttta caggcatcat
    13261 tgatttgatg aaaatgaaag cctatatcta taaagatgat ttgggaaaag aaattgtaga
    13321 agaagaaatc cctgaagata tgaaagataa agcagaagaa tacaggatgg caatgattga
    13381 agctattgct gaaaccgatg aagagctaat gatgctttat ctggaagggg aagaactcac
    13441 agaagaacaa ttaaagaaag ctctcagagc agcaacaata aaagttgaaa tcatacctgt
    13501 tctgtgtggt tctgcatata aaaataaagg tgttcaaaaa ctgcttgatg cagtcctgga
    13561 ttatttgcct gcaccaacag atataccagc aattaaaggt attattcctg acacaggaga
    13621 agaagttgaa agacattctt ctgacgagga accttttgct gcattggcat ttaagattat
    13681 gagtgaccct tatgtaggaa agttagccta ctttagagta tattccggaa tcataaattc
    13741 tggttcttat gttttaaact ccactaaaaa taagaaagaa agaatcggac gtattttgca
    13801 gatgcatgca aatcaccgaa atgaaattga caaagtatat tccggagata ttgcagcagc
    13861 tgtaggttta aaattcacta caacagggga tactctttgt gacgagcaac atccggtgct
    13921 tttggaatct atggtcttcc ctgaacctgt tatttcagtt gcacttgagc caaaaacaaa
    13981 agcaggtcaa gaaaaaatgg gtatagccct ttcaaaactg gctgaagaag acccaacctt
    14041 taagacttat accaatcagg aaacaggtca gaccattatt gcaggtatgg gtgaactcca
    14101 tcttgaaatt attgttgacc gcttactgcg tgaatttaaa gtggaagcca atgttggtga
    14161 gccacaagtt gcatacaaag aaactatcca aaaagcagtg gatgttgaag gtaaatttgt
    14221 aagacaatct ggtggtagag gacagtatgg acattgtaaa gttaagtttg agccactgga
    14281 tccaaatgga gaaaaaacct acgaattcga aagccaggtt gttggtggag ctattcctaa
    14341 agaatacatt cagccaatta atgatggtat tcaggaagca atgcagagtg gtatattagc
    14401 aggatatcca gtacttggtg taaaagcagt tgtatacgat ggttcatacc atgaagttga
    14461 ctcatctgaa atggcattta aaattgcagg ttcaatggca tttaaagaag ccatgaaaaa
    14521 aggtgatccg attcttcttg aaccaataga aaaagtagac gtaacggttc ctgaagaata
    14581 catgggtgac gttattgggg atctcaattc ccgtcgcggc cgtatcgaag gaatggaagc
    14641 aagaaacgga ttacaaatta ttcatggatt tgtgccattg tctgaaatgt ttggatatgc
    14701 aacagacctt cgttctaaaa cacaaggtcg tggaacttat tcaatggaat tccatcatta
    14761 tgagccggtt ccaaagaaca ttcaggacaa aatagtctcc ggcaaaggaa aagctgaata
    14821 aggcttgaaa tgtaggaaga cttgaaatat aataaaaaat gaattcaaat ggcaaataaa
    14881 aggaggatta ttaaaaatgg caaaagcaaa atttgaaaga acaaagcctc acgttaacat
    14941 tggaacaatt ggacacgttg accatggtaa aactacttta acagcagcta ttacaaaaac
    15001 tttacatgct agatatcaat taggagaaga aataccattt gatcacattg ataaagctcc
    15061 agaagaaaaa gaacgtggaa tcacaatttc aacatctcac gttgaatacg aaacaccaaa
    15121 tcgtcactat gcacacgttg actgcccagg acacgctgac tatgttaaaa acatgatcac
    15181 tggtgcagct caaatggacg gagctatcat tgttgtggca gctactgatg gtcctatggc
    15241 tcaaacaaga gaacatattc ttttagctcg tcaggtaggt gttccttaca tcgttgtatt
    15301 tatgaacaaa tgcgacatgg ttgatgaccc agaattactt gacttagtag aaatggaaat
    15361 ccgtgactta ttaagcgaat atgacttccc aggagatgaa attcctgtaa tcagaggatc
    15421 tgctcttaaa gcattagaag atccatcaag tgaatgggga gacaaaatca ttgaactttt
    15481 cgaagctgtt gacagctata ttcctgaacc acaacgtgaa gttgataaac cattccttat
    15541 gcctgttgaa gacattttct caattacagg tcgtggaaca gttgcaactg gtagagtaga
    15601 acgtggcgta cttaaagttc aagacgaagt tgaaatcgtt ggtttaactg acgaaccaag
    15661 aaaagtagtt gtaactggag ttgaaatgtt ccataagatg cttgaccagg cacaagctgg
    15721 tgacaacatc ggtgcacttc ttcgtggtgt tcaaaaagat gaaattgaaa gaggtcaagt
    15781 attagctcaa cctggaacaa ttacaccaca tacaaaattt aaagctcaag tatacgttct
    15841 taagaaagaa gaaggtggac gtcatactcc attcttcaca aactacagac ctcaattcta
    15901 tttcagaaca actgacgtaa caggcgttat caaacttcct gatggcgttg aaatgtgcat
    15961 gcctggagat aacgttgaaa tgacaattga acttattgct ccaattgcta tggaaaaagg
    16021 acttcgtttt gctatccgtg aaggtggtaa aacagttgga gcaggttctg taacagaaat
    16081 tattgaataa taagctgacg attaaaaata aatttaagtc ataaaaatca catcccatgg
    16141 gatgtgattt ttatgacaaa aaaataaaaa aaaccttgca aaaatctaat ttttattgta
    16201 tactttataa tgttgtgaca ttgatagtga tgatgtgaaa ggttgccact cgagtgggaa
    16261 attttcacgg agcgaatgtc cagttcgaga aacaggacga caaggtcact gtacagtaag
    16321 acggagatgt attcataatc catagggcat taagatacac ccggataagt gtgcagtcac
    16381 cacttgtcgt acgaaaacaa agtgcgaaaa ggaggggact ttttttatgg caaaccaaaa
    16441 aatcagaatc agtctaaaag cttatgatca caaattaatc gatcaatctg ctgcaaaaat
    16501 cattgagaca gcaaaaaaga ccggagctaa agttagtgga ccaatacctt tgcccactaa
    16561 aaaggaagtt ataacgattt taagagcggt tcacaaatac aaagattcca gagaacagtt
    16621 tgaaatcaga actcataagc gattaattga tattgtggtt ccaacaccta agactgtaga
    16681 tgcattaatg cgtcttgact tgccagcagg tgtagacatt aaagtggatg taaaataatt
    16741 aagaaaaata gattcgttcg cgatattatc agatgattat cggcataaga atctatctag
    16801 aatgattaca gagaagctgt aatccgctgt agattaatag gaggtgtaaa aatgaaaaaa
    16861 gcaatattag gtaagaaaat tggtatgaca caaatatttg atgaaaacgg aaagcatgtt
    16921 cctgtaactg ttattgaagc gggaccttgt gtggtcgtac aaaaaaagac gactgagaac
    16981 gatggttata atgccattca agtaggtttt cttgatgtga aagaaaatcg cgttaataaa
    17041 ccattaaaag gtcattttga aaaagctggc gttacaccaa aaagaacatt aaaagaattc
    17101 agattagacg atatcagttc ttatgaaata ggttctgaaa taaaggcaga catttttgcg
    17161 ccaggagata aggtagacgt aactggtatt tctaaaggaa aaggatatca aggtgttatc
    17221 aaaagacaca accaaggcag aggacctatg gcacacggtt ccaaatatca cagaggcgtt
    17281 ggtgctatgg ccggagcttc ttatcctggt agagttttaa aaggtaaaaa acttcctgga
    17341 catatgggtg cagaaaaagt tactgtacaa aatttagaag ttgttcgtgt gtaccctgaa
    17401 aagaatttaa tacttgtaaa aggagcagta ccaggaccta aaaagtcaat tgttatgtta
    17461 aaagatagtg taaaaaatgc atagtgataa ctggagaaag gaggagtcat gatggctaaa
    17521 gtagctgtgt ataatatgga tggacaacaa gttgatgaaa tagaattaaa agatgagatt
    17581 tttggagtag aagttaacga acatataatg cacgcggcag tagtacaata tttggcaaac
    17641 caaagacaag gtactcaaag tgctaaaaca cgtgcagaag tccgcggagg cggaagaaaa
    17701 ccctggagac aaaaaggcac aggaagagca agacaaggtt ctatccgtgc accacaatgg
    17761 actggcggtg gtgttgtatt tgctccgaag ccaagagatt attcatttaa attaaataaa
    17821 aaagtaaaaa gattagcatt aaaatcagct ttaacaagca aagttcaaaa taataattta
    17881 attgtgttag atgaacttaa attagatgaa atcaaaacaa agaacatgaa gaaagtttta
    17941 gataatctta aagtaagcaa agcattaatt gtaactgaga gcgataataa aaatgttatt
    18001 ttatctgcaa gaaatcttcc caatgtaaag actgctgcag taaatacaat taatgtatat
    18061 gatatcctaa agtacgaatc ttttgttgta tctaaagatg tacttcagac gatacaggag
    18121 gtgtatgcat aatggcagat ttaaaatact atgatgtcat cttgaaaccg gttattaccg
    18181 aaaaaagtat gaacctgatg gcagaaaaga aatacacctt ccttgtacat cccgcagcaa
    18241 caaaaaatca aatcaaagaa gctgttgaaa aaatgtttga aggtgtaaaa gttaaaaagg
    18301 taaatacaat gcttcaagat ggtaaaacaa aaagaagagg aatgactatt ggcaggactg
    18361 ctaaaactaa aaaagcaatt gttcagttag cagaagaaag taaagatatt gaattctttg
    18421 aaggtatgta attagaaatg aaacaccagg tagcgtgtaa caagaattga aaggagtgaa
    18481 agatatgggt attaaaacct ataaaccata taccccttcg agaagacata tgacttccct
    18541 gacctttgaa gaaataacta agagcactcc ggagaagtcc ttagttgtat ctttaaagaa
    18601 aaattcagga agaaacaatc aaggtaaaat tactgttcgc catcgcggag gcggagcaaa
    18661 aataaaatat agaattatcg attttaaaag aaacaaagat ggaattccag caagagtagc
    18721 tgcaattgaa tatgatccaa acagaacagc taatattgct ttattacact atgctgacgg
    18781 tgaaaagaga tatatccttg ctccaaaagg cttaaaagtt ggagataaga ttatgagtgg
    18841 tgaaacagct gaaatcagag taggaaacgc acttcctatg aaaaatattc ctgttggtac
    18901 aaccattcat aatattgaaa tgaaacctgg taagggtgga caactcgttc gttcagcagg
    18961 agcagaagct caattattag caaaagaagg aaagtatgca accgttagat taccttccgg
    19021 agaaaccaga ttacttccaa ttgagtgtag ggctactata ggacaagtag gtaacattga
    19081 acatgaactt gttactatag gtaaagcagg aaggaaacgt catatgggta tccgtcctac
    19141 agttcgtggt tctgtaatga atcctaacga ccatcctcat ggtggtggtg aaggaaaggc
    19201 accaatcgga cgcccaacac cagttacacc ttggggtaaa cctacattgg gatataaaac
    19261 aaggaaaaag aataaggcat ctaacaaata tattcttcat agaagaacga aataagtttt
    19321 ctaatataca ttatagagtc ggaaggagga taattaatgg ctcgttctct taaaaaagga
    19381 ccttttgccg atgaacattt attaaagaag attgaagaaa tgaataaaag cggagaaaag
    19441 aaagttatta aaacatggtc ccgccgttct acaatttttc ctcaaatggt tggtcataca
    19501 atagctgtac atgatggtag aaaacatgtt ccagtatata taacggaaga tatggtcgga
    19561 cataaattag gcgaatttgc cttaacaaga acttacagag ggcatggtaa aaatgctgaa
    19621 aagaaatcag gagctcgtta agattgatag ataaaaggag ggttgcttcg tggctaaagg
    19681 acatagaagt caaataaaaa gagaaagaaa tcaaaataaa gacacaaggc caaaggcaca
    19741 tcttagatac gcgagagtat ctgcctctaa ggctaagatt gttcttgata ccataaaagg
    19801 caaaaatgct aatgaagcgt tagccattct tgcttataca ccaagaaagg ctgcaagaat
    19861 tatagaaaag ttattaaaat cagccatagc aaatgctgaa aataaccatg agttagacag
    19921 agaaaaatta tttatcgaag aagcgtatgc aaatcaggga ccaaaaatga aaagaatccg
    19981 tcccagagca caaggaagag cataccgtat tgataaacaa accagtcata tttcaattgt
    20041 actgaatgaa agataataag gaggttgagt atgggacaaa aagttaatcc tcatggacta
    20101 agggtcggta taatcaaaga ctggagcaca aaatggtacg cttctaagca agaatatgcc
    20161 gatttgttag ttgaagatta taaattaaga gattatatta aaaataagct ttatgatgca
    20221 ggaatatcta aaattgatat tgaaagagcg gctgaaagag ttaaaataaa cattcatacc
    20281 tccaaaccag gtattgttat aggtaaagga ggttcttcaa tagaacaact tcgtaatgag
    20341 cttcaaaaat taacaaaaca caaagtaatg gtaaacatta ttgaaattaa gaaacctgaa
    20401 atagatgctc aattagtagc tgaaaacgtg gcgcttcaat tggaaaaccg tgtttccttt
    20461 agaagagcaa tgaaacaagt aatggcaaga gcaatgaaaa atggagcaaa aggtattaaa
    20521 gtagcttgct caggtcgttt ggctggtgct gaaattgcac gtactgaaca ttatcatgaa
    20581 ggcaccatac ctcttcaaac tttaagagca gatattgatt atggttttgc tgaagcagac
    20641 acaacctatg gaaaaatcgg tgtaaaggta tggatttata aaggtgaaat tcttcctcag
    20701 aaggccaaaa aggaaggaga gattaataat gttaatgcct aaaagggtaa agcatcgtaa
    20761 acaattcaga ggacgcatga aaggcagagc tacccgtggt aataaaatta cttacggaga
    20821 gtttggtctt gttgctttag aaccaggttg gattacctcc aatcaaatag aagcagcgcg
    20881 tgttgctatg acaagatata tcagacgtgg aggtaaagtt tggattaaaa tttttccaga
    20941 taaacctgta acacaaaaac cagcagaaac ccgaatggga gcaggtaaag gctctccaga
    21001 atactgggta gcagtagtaa aacctgacag agtaatgttt gaaatagcag gagtaaacca
    21061 ggaaacagca agagaagctt tgcgtcttgc aatgcacaaa ttacctatta aatgtaaaat
    21121 cgtatcacgt gcagatcaag aaatggacgg tgatagcagt gaaaagtaaa gcatatttag
    21181 atgaattaag aaataaaacc ttagatgaat taaaatctga tttggtttct gctaaaaaag
    21241 aacttttcaa tttaagattc caaaatgcaa caaatcaatt aagcaataca gcaagaatta
    21301 aagaagtaag aaaaaatata gcaagaattc aaaccgttat ttcagaaaga gcaaaggcct
    21361 agtaactatt gcgaaaggag gaattcacgg tgggagaaag aaaaccccgc aagacattga
    21421 ttggtaaagt tgtcagcgac aaaatggata agacgattgt tgttgctgtt gaaaataatg
    21481 taaaacaccc attatacgga aaaattataa aaagaacata caaattaaaa gcccatgatg
    21541 aaaataacga atgtaaaatt ggtgatcttg taaaagttat ggaaacaaga cctctctcta
    21601 aagaaaagag atggagattg gtgtcaattg ttgaaaaagc aaaataatac tgctcggaag
    21661 gagggtaaat catgattcaa caagaaagca gattaaaggt tgcggataat actggtgcga
    21721 aagaattatt atgcatccgc gtactgggcg gttctaaggt tagatatgct aatgtgggag
    21781 atatcatagt tgcttctgtt aaagatgcaa cacccggcgg tgttgtaaaa aaaggagatg
    21841 ttgtaaaagc agttgttgtt agaaccgtga aaggcgttag aagaccagat ggttcatata
    21901 taaaatttga tgaaaatgca gctgtaatta taaaagaaga taaaaaccca agaggaaccc
    21961 gtatttttgg accagttgca agagaattaa gagaaaagaa atttatgaag atattatcct
    22021 tggcaccaga agtactataa ggaggtgttt tgagtgaaaa aagacaagaa agaacagaaa
    22081 attaaaacca gacttaaagt tggcgataag gtacgtgtta tttctggaga atgtgaaggc
    22141 aatgaaggaa aaattttaaa aatagatcgt aaaaccggcc gtgttatcat agaaggtgta
    22201 aatatgatta caaagcatga gaaaacgaat gctatgggac agggcggaat tatcaaacaa
    22261 gaaggtccta ttcatatctc aaatgtaatg tatcttcaca atggtaaacc aaccagatta
    22321 ggtgttaaag ttgttgaaga agagagaaac ggccgtatga aaaaggttcg ttacagagtt
    22381 gcaaaatcta ctggtgaagt gattgattaa cagcggaagg agggtaagtt ttgagtagag
    22441 taagagaact ttatgaaaac gaaattgttg aacagatgat gaaaaaattt ggatataaaa
    22501 ataaattaga agtacctaaa ctcgaaaaaa tagtcatcaa catgggagtt ggtgaagcta
    22561 aagacaatcc aaaagcatta gaagctgcag ttaacgattt aactgctatt tccggacaaa
    22621 aaccagttat tacaaaggct aaaaaatctg ttgcaaactt taaaattcgt acaggtatgc
    22681 caataggatg taaggtaacc cttcgtggaa gaagaatgta tgactttgta gatcgtttaa
    22741 tttcattggc attacctcgt gttcgtgact ttagaggagt taatcctgat tcttttgatg
    22801 gaagaggaaa ctatgcatta ggaatcatag aacaattaat ctttccagaa attgattttg
    22861 ataaagtaga taaagtcaga ggtatggata ttatcttcgt taccacagca aaaacagatg
    22921 aagaagcacg tgaattgcta acactgttcg gaatgccatt cagaaagtaa tagaaggagg
    22981 gtaacctatg gcgaaaaagt caatgattgt taagcaacag cgtaagccaa agttttctac
    23041 aagagcttat aatcgttgca gaatttgcgg aagacctcac gcatatttaa gaaagtttgg
    23101 agtatgccgt atttgtttca gagaattggc atacaaaggt caaattccag gagttaaaaa
    23161 agcaagctgg taaaaggaag gaggtacatt caatgacaat gactgatcca attgctgata
    23221 tgttaacaag aatcagaaat gctaatattg caaaacatga tacggtagat atccctgcat
    23281 caaagatgaa acaagcaatt tctgatatat tattaaacga aggttatatt aaaggttatg
    23341 aaattataga agatggtccg aaaaaaacaa tccgtattac attaaagtac ggcaaagata
    23401 agaacgaaaa agttatttcc ggtcttaaaa gaatttcaaa gccaggtctt agagtatttg
    23461 ttggaaaaga cgaattaccg aaagttttag gtggacttgg aactgcaatt atttctacaa
    23521 gcaaaggcgt agtgacagat aaagaagcca gaaagttagg cgttggtgga gaagtagttg
    23581 catttatctg gtaaggatat aacgatgact aagatagata aattatggat taattgctta
    23641 aggaggtgcg accatgtcac gaattggtaa attaccgatt gcgattccat ccggtgttga
    23701 agtaaaaatc ggagaaggaa atctggtaac cgttaaggga ccaaaaggta cattagaaag
    23761 aaaattatca ccagagatgt taattacaat tgaagataat caggtaatcg taaaaagacc
    23821 tagcgattta aagaagcata aagctcttca cggcttaaca agaacacttg ttaataacat
    23881 ggtagaagga gtaaccaatg gtttcacaaa ggaattagaa atcaatggcg ttggatacag
    23941 agcacaaaaa caaggcaaaa aacttgtttt atcacttgga tattcacatc ctgtagaaat
    24001 ggaagaccct gaaggaattg aaagtactgt tgaaggaaac aaaattattg ttaagggtat
    24061 aaataaagaa aaagttggtc aatacgcagc ggagatccga gctaaacgtc caccagaacc
    24121 atataaagga aaaggtatta agtatgttga tgaagtaatc agacgtaaag aaggtaaaac
    24181 aggcaagtaa ttagtaaagg ggtgaatccg tatgataaaa aaagaaccac gttctaaaat
    24241 tcgcgtaaat aaacacctga aagtgagaaa gaagattttt ggaacatctg aaagaccaag
    24301 attatctgtc ttcagaagcg ataaacatat ttacgcgcaa attattgatg atacaaaagg
    24361 tcatacttta gttgctgctt caacagttga aaaagacatc aaatcaaaaa ttgaaaaaac
    24421 caataatgtt gatgcagcaa aagtagttgg agaaatgata gctaaaagag cattagataa
    24481 aggcgtaaaa caagttgttt tcgatcgtgg cgggtatata tatcacggta aaataaaagc
    24541 tttagctgat gcggccaggg aagctggatt agaattctag taaaggaggg aatggaatgg
    24601 caaaggctaa aatagatgtt aattctttag aactcaagga aagagttgta acgataaaaa
    24661 gagttaccaa agttgttaaa ggcggacgaa acttcagatt ctcagctttg gttgttgttg
    24721 gtgacgaaaa cggtcatgtt ggagcgggat taggaaaagc cgcagaaatt cccgatgcaa
    24781 ttagaaaagc aattgaagat gcaaagaaaa atatcattta tgttcccaga gataagaatg
    24841 atagcttacc tcatgaattc ataggtgaat ttggaagttc aagggtatta ttaaaaccag
    24901 ctcctgaagg aacaggagtt attgcaggtg gtccagtacg tgcagtactt gaattagccg
    24961 gtgttcgaaa tgtcagaaca aaatcccttg gttccaacaa taagcgtaat gttgttaatg
    25021 caaccatgga aggattaaag aacattaaag attcttcaga agttgctaaa ttacgcgata
    25081 aaactgtaga agagttatta ggttaaggag ggattgcagt ggcagataaa ttaagaataa
    25141 ctcttgtaaa atctactatc ggggcaaaac ccaaacacaa agagatagta agatccctgg
    25201 gtcttaataa gttaaataag accgttgaac aaaaagacaa ccctgctatc cgaggaatgg
    25261 tagaaaaagt ttctcatttg gtgaaagttg aagaaatata gattgaggat ttaaaggagg
    25321 tgccgagatg aacttaagtg tattaaagcc caatgaagga tctaaacaca gccgaaccag
    25381 aaaaggtaga ggacacgctt caggtctggg aaaaacatca ggaagaggac ataaaggaca
    25441 aaaagcgcgc tcaggcggcg gtgtaagacc tggttttgaa ggtggacaaa tgcctcttta
    25501 cagacgactt ccaaagagag gatttacaaa tataaactct aaagaaatcg ttgcaataaa
    25561 cgtagaccgt ttaaatatct ttgaagatgg cacagaggtt actgtagata ctttaaaaga
    25621 agcaggtatt attaagaatc caaaagacgg agtcaaaatt cttgggaatg gcgacctaaa
    25681 taaaaaactg acagtgaagg tgaaccaatt tagtaaaaag gcagttgaaa aaattgaagc
    25741 tgctggcgga aaagcagagg tgatttaggt gactgaagcc ctaaaaagcg ttaggaatat
    25801 ttggaagata cctgatttaa ggaaaaaagt aatatatacc ttgctgatgt tgtttgtaat
    25861 ccgtattggt gcacacgttc ctgttccatt tgtcaatgca gctgctgtaa gggaagttct
    25921 tgagatgcaa ggtacaggtc ccttaaaact gattaatatg atttctggtg gggcatttga
    25981 agatatgacc atttttgcat tgggcattgt accctacatt aacgcatcga ttattttcca
    26041 gcttcttcaa attgcaatac ccaaactgga ggaaattgca aaagaaggag aagaaggaag
    26101 aaagagaatt gcaaaatata caagatattt aaccattgcc ctggcagctt tacaagcctg
    26161 gggtacaact tttgctctta gaaatcagaa catattaatc tacagtaatt tgtggacttg
    26221 gattgttgta attgttacct ttgtggcagg aacaactttt cttgtgtggg ttggtgaaca
    26281 aattactgaa aagggagtag gtaacggtat ttcactgata atctttataa acattgtttc
    26341 aagattaccg ttagctatta cgactttgtt tagctataaa gacaaatata tcagcattat
    26401 tttattaatt gtcatctttg tgttaatgat cgcttttgtt gtattggttc aggatggaga
    26461 aagaagaatt ccagttcaat acgctaaaag agttgcagga agaaggatat atggcgggca
    26521 atcaacgcat attcctctta aggcaaatct tggaggggta atgcctatca tttttgcaat
    26581 gtccatactc caatttccaa taatgatgac taattttttc acttccaatc ctacaggact
    26641 ttggggaaaa attataactt atattaactg gacccatcca gttggggcaa tactatatgc
    26701 agttttaata tttggctttt cattcttcta ttcaactgtg gcatttaatc cggttgatgt
    26761 tgcagacaac atgaaaaaga atggtggttt tatcccagga atcagaccag gtaacccaac
    26821 tgcagagtat ttaaggaaaa ctcttaacaa tattaccgta gttggagctt tattcttaac
    26881 gataattgcc ttactgccta ttgtattgca atggatattt aagatgagta ttggatttgg
    26941 tggaacatcc ataatcatcg tggttggtgt tgctcttgaa accgtacagc aaatagaagc
    27001 acaaatgtta atgagacatt ataaaggctt tttaagctaa gaataacagg agggaacctt
    27061 ttatgagact tataatgttg ggtgctcctg gagcaggaaa aggaacgcat gcattacttc
    27121 tttccaagga atataatatt cctcaaatat caacaggcga tattttaaga agtcatataa
    27181 aaaatgagac agatttaggg aaaaaagcta aaaaatatat ggataaaggc ctgttagtac
    27241 cagatgagtt agtcgttgaa attgtaaaaa agcgtattca ggaagaggat tgtaaggaag
    27301 gttttatatt ggacggtttc cccagaacaa ttcctcaggc tgaagccttg gataaagctt
    27361 tagaagaatt agctattgct ttagataagg tcgttaatat cgatgttccc gatgaagtaa
    27421 ttattaagcg tatggctggc agaagagttt gttctaactg tggtgcttcc taccatattg
    27481 aaaataaaaa gcccttaaaa gaaaatatct gtgatgaatg tggcagtgaa ttaaaacaga
    27541 gggaagacga taaagaagaa acggtcagaa aaagattaca ggtatacgaa gaacaaacaa
    27601 agccattgat tgaatactat gaaaaaaggg gaatactttt aacgatagaa ggcgatggaa
    27661 gcgttgaaga aataggaaac aagatcaaaa atgcattgga agtgagcaaa taatgggaat
    27721 aactattaag actgacgagg aaataagaaa aatccgggaa gcaggcagaa ttttggcaaa
    27781 aacacaagaa cttttagcca gcaagattaa agcaggtatt acgaccatgg aactggatca
    27841 gattgcagaa gaatttatac gtagtcaggg tgcaactcct tcatttaaag gattatatgg
    27901 ttttccagct tcaatctgca catcgatcaa tgaagaagtt gttcacggga ttcctagtgt
    27961 aaagaggatg cttaaagagg gagatattat tagtatagat gttggcgtat gtcttgatgg
    28021 atatcattcc gatggagcaa aaacgtatgc cattggtgaa gtagcagaag aaacaaaaag
    28081 attaatcgaa gtaaccaaac aaagctttta tgaggggatt cggtttgcaa gacctggaaa
    28141 tcatttatat gaaatttcat cagccataca aaaatatgta gaggctaacg gattttcggt
    28201 tgtaagagac ttaaccggcc atggagtagg cagaaaactt catgaggaac cgcaaatacc
    28261 aaactttgct gttcctggcc ggggaccgaa actcgtaaaa ggcatggtcc ttgctattga
    28321 acctatggtg aatatgggat cttaccaggt aagaatatta gcagacaaca gcgtggtaac
    28381 cagagatggt tctctttcag cacattatga acataccatt gcaataacgg atggagaacc
    28441 tgaaattctt actactttgt gataaagagg ttaggtgagc taaggatgaa tgagtactcc
    28501 attgggcaag ttgtgttttc taaatctgga cgtgacaaag gaagagcttt cattgtaata
    28561 caaattgaaa atgaatatct ctatctcgta gacggagatt taagaaaact aagtaagcca
    28621 aagaaaaaga agaacaaaca tgttcaaaaa acaaattata taatagaatc tattaagaaa
    28681 aagatagaag atggcgtaga acttaatgat gcggaaataa gaaaaagtct tgaaccttat
    28741 agtccttctt ctagaaatgc aataaatgag gaggcatgat accttggcga aaagtgatgt
    28801 tattgaagtt gaaggaaccg tgcttgaaaa attacctaac gcgatgtttc aggttgaatt
    28861 agaaaacgga cacaaaattt tggcgcacat ttctggtaaa cttcgaatga attttattcg
    28921 cattttaccg ggagataaag ttacagtaga actttctccc tatgatttaa caaaaggccg
    28981 gattgtttgg agatctaaat aatttcggca gtagaaagga gataagcaaa tgaaagtaag
    29041 accttcagta aaaccaattt gtgaaaaatg caaaatcatc aaaagaaaag gtcgtatccg
    29101 tgttatttgc gaaaatccaa aacataagca aaaacaaggc taacaaaaga catgcttgct
    29161 aaaggggcga gcgaccttaa gcaagctgat agaattcaga taaaacaatt gattaattgt
    29221 gtgatatatt atataatgat aatttgtgga gaatataaac atttgtattc tttagcggct
    29281 gcatatttgc tgatagaagt gtagatatat aataaattaa aaagaaaagc taatagtatt
    29341 aggaggtgca agtatttaat ggcacgtatt gcaggtatcg atttgccaag agataagcgg
    29401 gttgagatcg gtcttactta tatatatggt attggccgtg caagttcaaa tcgtatatta
    29461 aaggaagctg gagtaaaccc ggatacaaga gtaagagatt taaccgatga cgaagtatcc
    29521 agaattcgtg agatcattga aaaaactcaa gtcgttgaag gagatttacg cagagaagtt
    29581 gcattgaata ttaaacgact aatggaaatt ggatgttata gaggtattcg tcatagaaga
    29641 ggtcttccag ttagaggaca aaacacaaag aataatgcta gaacacgaaa aggacccaga
    29701 agaacggttg caaacaagaa aaaatagtgc ataagggagg ttaaaattga atggcaaaga
    29761 aagtagctaa aaaagcaact agccgtagac gccgtgagaa aaagaatgtc gacagaggac
    29821 aagcgcatat tcagtctact tttaataata caattgttac cttaaccgat atggccggaa
    29881 atgctctttc ttgggcaagt gcaggacaat taggtttcaa aggttctaaa aagtctactc
    29941 catatgcagc tcaaatggca gcagatgctg cagcaagagc agcgatggaa tatggtttaa
    30001 aatcagttga agtgttggta aaaggaccgg gatccggcag agaagcagct attcgtgcgc
    30061 ttcaagcggc aggcttagaa gtaactatga ttaaagatgt tacgcctgtg cctcataatg
    30121 gatgtcgtcc accaaaacgc agaagagttt agttttagga ggtgtagaga taaatggcta
    30181 gatatatagg acctgtgtgc agattatgcc gtagagaagg gcaaaaactg tacttaaagg
    30241 gagaaaagtg tttttcacaa aagtgtccag tagttagaag accatatgca cccggacaac
    30301 atggtcaagt aaataaaaag ctttcagagt acggattaca gcttcgcgaa aaacaaaaag
    30361 caaagagaat ttatggtatt cttgaaacac aatttgctaa ttactttgaa gaagcagacc
    30421 gtattcctgg cattactggt gagaacttac ttcgcatact tgaaatgcgc ttagataatg
    30481 tggtttaccg tttaggatta ggacgttcca gaacagaagc cagacaagtg gttcgccata
    30541 accatatttt agtaaacggt aaaaaggtaa atattccttc ttaccaagta aaagttggag
    30601 atgttattga agttaaagaa aaatccaaag gacttcaaag atttaaagat ataacagata
    30661 ctactgcatc cagaatcgta cccgaatggc ttgaagctga catggacaat ttaaaaggta
    30721 aagtagttgc tctaccagaa agagatcaaa tcgatattga tattcaagaa acacttatcg
    30781 tagagcttta ttcaaaataa tagttattag acaaattgtc accctcagga atattaataa
    30841 agaagaagga gggtttatcg ggtgtttgaa tttgaaaaac ctcgtattga gattgccgaa
    30901 atgtcggaag atggaaaata tggacgtttc gttcttgaac cccttgaacg gggctatgga
    30961 acaaccttgg gtaactgtct aagaagagtt ttactttctt cccttcctgg tgttgcggtt
    31021 agcaatgtaa aaattgaagg ggtactccat gaatttagta caattcctgg cgtaaaagaa
    31081 gatgtaccgg aaattatatt aaatttaaaa ggccttgcgc ttaagaataa tagtgacagc
    31141 aatgaaccta aaattgctta tattgaagca gaaggagaag gcgaagtcac tgccgcagat
    31201 atcaaagtgg attctgatat agaaatcatc aatccggatc ttcatattgc aacattaagt
    31261 ggaggccctg acagcagatt gtttatggag atgaccataa ccagaggtag gggttatgta
    31321 cctgcagaaa aaaataaaat gattaatcat gctataggag taattcccgt agattctctt
    31381 tatacaccag ttagccgtgt gaattttacg gtagaaaata caagagtagg tcaaattaca
    31441 gactatgata aattgacctt agaattgtgg acgaatggaa ccattaaatc ggatgaagca
    31501 gtaagtttgg ctgcaaaaat cttaagtgaa catttgaatt tgttcattga tttgtctgat
    31561 aatgctaaga atgcagaaat aatggttgaa aaagaagaag atcaaaaaga aaaagttctt
    31621 gaaatgagtg ttgaagagct tgatttatcc gttcgttctt ataactgtct gaaacgtgca
    31681 ggtattaaca cagttgaaga actgattagc cgtacagaag aagaaatgat gaaagtacgt
    31741 aatctgggta aaaagtcctt agaagaagta cttaacaaac tcgcagaatt aggtctttca
    31801 ctgaagccta gtgatgatta gttgcttggt atatttatgt aaaggaggtt gaatgatggc
    31861 tgggcataga aaattaggtc gtcctacttc tcagcgtaaa gcattactga gaaatcaagt
    31921 aaccaattta ctttatcatg gtagaataaa gacgacagaa gctaaagcaa aagaagtaag
    31981 aaaacttgcc gaaaaattaa taacccttgc tataaaggaa aaagacaatt acactgaagt
    32041 aaccgttaaa gcaaaagttg ctagaaaaga cgcaaagggt aaaagagtaa aagaagttgt
    32101 aaacggcaaa aaagtagatg tatttgatga agtagaaaaa actattaaaa aggataatcc
    32161 ttccagactt catgcaagaa gaagaatgtt acaaattctt gttcctgtaa ctgaaaccag
    32221 taaaattaag aaaaaacaaa acaccaaaaa agttgatatg gtggaaaaac tcttttcaga
    32281 catagcacct aagtatgcag accgtaacgg aggttatacc agaattatta aacttggacc
    32341 ccgtaaaggt gacggtgctg aagaagtaat tattgaatta gtttaagatt ttgtagggat
    32401 taggatgtaa tcttaatccc tataaatatc ttaataattg aagattaatt atttggtaaa
    32461 gacgaaaatc tccaaaaagt ctttattcaa aataattaat tttgaattat taaggctccc
    32521 taaggttggt gataatatga aagaaatcgt aaaaagcagg ggacttatat acgaatacta
    32581 cacccataat gagcgtggcg aaattgaagc gacgaacagg gctctggaca ggttggattt
    32641 agatattaaa caaggggaat ttgttgtcat tttaggccat aatggttcag ggaaatcaac
    32701 ccttgccaag cacatgaatg ccattttaca tcccacagga ggaaccctcc ttattaaagg
    32761 tatggatact aaggacgaag aaaacttatg ggatatcagg caaacagcag gaatggtctt
    32821 tcaaaatccc gacaatcaga ttattgctgc aattgtagaa gaagatgtgg cttttggtcc
    32881 agaaaattta ggtgtagagc ctggtgaaat caggaaaaga gttgatgaag cagttgctgc
    32941 tgttcaaatg acagagtata tgactcactc tcccaacttg ttatccggag gtcaaaagca
    33001 acggatagct atagcaggag tattggcaat gaagccagaa tgtattattt tagatgagcc
    33061 tacagcaatg ttggatccac tgggcaggaa agaagtcctg gagacggtgt ataaattaaa
    33121 taaagaagaa aatataacca ttattcacat tacacactac atggaagaag cgattaatgc
    33181 tgacagagtt attgtaatgg accatggtca aattgttatg gatggcaagc ccaaagaagt
    33241 ttttagtcag gtggacaagt taaagagctt gggtttagat gttcctccag ctactgaatt
    33301 ggcgtattat atgcaaaaag aaggcattga tatttctaca gaaataatat caattgaaga
    33361 gatggtggat aaaatatggc aattatattt aacggagtga gtcatgtata tggtgaaggt
    33421 tcacctttta aaagagttgc tatcaataac ataaaccttc atattaaaaa aggagaattt
    33481 ataggcatta ttggccatac tggctctgga aaatctactc ttattcagca ttttaacggc
    33541 ttattgaaac caactgaggg agaagtaatc atactaggta aaaacttaaa tgataaagag
    33601 ctttcattaa aagaaattcg tcaaaaagtt ggccttgtct ttcaataccc ggaacaccag
    33661 ctttttgaaa caactatttt taaagacgtg gcattcggac cagaaaatat gggtttatca
    33721 aaagaagaag tcgataagag agtcagagaa gctttagaaa tggtaggcat aggggaagac
    33781 atgtatgaca aatccccttt tgatttgtcc ggaggacaaa aaagaagggt tgctattgca
    33841 ggagttctgg caatgaaacc tgaaattctg attctggacg agccaacagc aggtcttgat
    33901 cccaggggca gggatgaaat tctgggacaa attcaaaagc tgcataagtc cttaggtatt
    33961 acagtcattt tagtatctca cagcatggag gacattgcaa aactggttga cagaattatt
    34021 gtaatgtata agggtagaat tgctttagac ggttctccgg cgaaggtttt caaaaatgat
    34081 aaggaattgg aaaagatggg cttagcagca cctcaaatca tgtacatcat gaaagcattg
    34141 agaaaaaaag gccttgaagt tcctgtagat gtattaactg tagaagaagg cgctaaagca
    34201 attgcagcat tattgaaaag tcaaaagtag gtgctatgat gatacgagat attacaatag
    34261 gacaatattt tccagcagaa tcatggattc ataaattaga tcccagggtc aaaattatgt
    34321 caacccttat ttttatcatt gctctttttg tagccaaccg ctatataggc tatcttttta
    34381 catttatttt tatggccaca gttatttact tatcaaaagt acctgtaaaa tatatgctta
    34441 aaggtatcaa agcgatcctt gtcattatta tacttacggt tgctttaaat atttttatga
    34501 cacctggtga aaaggtgctt tggtcctaca aatctataaa tattactgcg gaaggcttat
    34561 atattgcagg gataatggca ttaagactga tatttttaat tattggttca tctgttttga
    34621 ctttaaccac atcaccaata caactaacgg atggtcttga aaaactttta aatccattta
    34681 aaaaaatagg gatcccagcc catgaaatag caatgatgat gacgattgcc cttcgcttta
    34741 tacccatatt attagaagag acagataaaa ttatgaaagc ccaaatggca agaggggctg
    34801 attttgaaag cggaggcatt attaaaagag ctaaaagcct gatacctctt ttggttcctt
    34861 tattcatttc agcttttaga agggcagacg aactggccat ggcaatggaa gcaagatgct
    34921 atcgtggagg ggaaaacagg accagaatga agcagcttaa aataaaaaag caagattatg
    34981 tggcaatggt aagcattggt ctgtttatgg gcataatgat tgtaagccgt tttatctaat
    35041 aggagttcaa agctttttga taaaaatatg gaggaaaata tgaaacatat tttactcacc
    35101 attgcatatg acggaagcaa ctataatggc tggcaaagac aatccaatgg aatagggata
    35161 caggaaaaag ttgaagaagc ctgttttaaa atttttggac aggagataag tgtcacaggt
    35221 gcaggaagaa ccgatgcagg tgtccatgcc ctggggcaaa gagggcttat caaagtggag
    35281 acttccattc ccacagacaa aattctctat gcgttaaatt cagtccttcc aaaagatatt
    35341 gtaatcagag ctgccaggga agttcctgag aactttcatc cccgctatga tgccagggaa
    35401 aaaacttatc gctatttaat attaaacgat aaatttccca ttccgcagta tagaaatata
    35461 agtactttta tttctgccca attaaatgta gaagaaatgg acaaaggagc gaaatacttt
    35521 ataggaaacc atgattttgc agccttttct tctgcaggaa gttcggtaaa gacaacagaa
    35581 agaacgatat tcgatgcttt agtgtataga gaagatccat ttgtcatttt tgaagtaaga
    35641 ggtaatggct ttctttataa tatggtaaga ataatggcag gaactttgat agaaataggt
    35701 attggaaaga aaaaagcttc ggacgtaaaa gacatcattt tatccagaga ccggagtaaa
    35761 gcaggaataa cagcaccagc ccaaggttta acattagtcg aaataaaata ttaaagttta
    35821 tataaaaaat atgatgaaat ttaattattc cttgacacac cagggtaaga tgtaatataa
    35881 tgttttttgt gtaattatgc cacttaaaat ccattagccc cgggttttaa atagagtcgt
    35941 gattaaagaa ggaataagga gggtttgaaa tgagaacaac ttacatggcg aaagcagaag
    36001 ctgttgaaag aaaatggtac gttgttgatg caaaagatat gacattagga cgtttgtcat
    36061 ctgaagtagc aaaggtactt cgtggaaagc ataaaccaac atatactccc catattgata
    36121 ctggtgattt tgttattgta attaatgcaa aagacattaa agtaacagga aagaaattag
    36181 accaaaagct ttacagacat tatacaggat atgctggtgg tttaaaagaa atttcactca
    36241 gagaccttct cgaaaagaag cctgaaaaag taatctatga agcagtaaga ggaatgcttc
    36301 ccaaaaaccg tttaggcaga aaaatgctta agaagcttcg tgtttatagt ggtcctgaac
    36361 acaagcatga agcacagaaa ccggaagtat tagaaattaa attttagtag tgaagggagg
    36421 atatccaaat ggctgaagca agatattatg gaacaggcag aagaaaaaaa tcaatagcaa
    36481 gagtatattt ggtacccggc aacggtaact ttaaaataaa taaaagagat attgaagaat
    36541 attttggact tgaaacacta aaagttattg caaaacaacc tttagtatta accaatacag
    36601 tagataaatt tgacgtatta gtaaatgttt atggtggtgg atacactggt caggcaggtg
    36661 ctattcgtca tggtattgca agagcattgc tccaggcaga tccagaatta agaccagtgc
    36721 ttaaaaaagc tggattttta acaagagatc caagaatgaa agaaagaaag aaatacggac
    36781 tcaaaggtgc aagaagagca ccacaattct caaagagata attggtgttt ctttacacac
    36841 gagtttgttt acaattacaa agtttgctta tcgcaagcgc
//
LOCUS       DAOSUD010000012        35758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_831_length_35758_cov_26.489035, whole genome shotgun sequence.
ACCESSION   DAOSUD010000012 DAOSUD010000000
VERSION     DAOSUD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 35758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35758
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_35758_cov_26.489035"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1153
                     /locus_tag="PLG49_03010"
     CDS             98..1153
                     /locus_tag="PLG49_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOQ16317.1"
                     /translation="MFRSKKRLLALGLTAVISLSSATALYASPVIKTLRAQYMGIKFS
                     YNGKIVDPGNQEPFVVDQTTYVPIRMVGELVGKNVQWDGNNKLISINDTVDQTLIQKD
                     LEIIQLKAEIESLKQQLEAAKKQTPPTQTEDKNTSKISLSNLEDDINEDYGELKGVVF
                     DIYLSGDSKEIEIEMETDLYRYKDYWDKLSEKDVEKHLEKICKAIWEVYDKAKIVGDL
                     YDIDEREYVLEFSTDSRKKLTVEYVNKLDLSKLEDELDNRYEHYLSPYIYLSLDIKGD
                     EEEIEYYLNVDYREWWDDLDDEQVKTLMGKIYDDIKKAAPNATSIEGYIYDTSRKSVI
                     AEYWRTKSGNAVFLPYY"
     gene            complement(1565..1852)
                     /locus_tag="PLG49_03015"
     CDS             complement(1565..1852)
                     /locus_tag="PLG49_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16318.1"
                     /translation="MINSIMDTRVRQEDGCYQINLKLPKDEYEMIYEGNYSNEIAADI
                     LKYYLEMRGDEGRPKNIVIKQSENPNMLEIQAVLEYVQNEHTDYNHQVNYF"
     gene            complement(1938..4769)
                     /gene="uvrA"
                     /locus_tag="PLG49_03020"
     CDS             complement(1938..4769)
                     /gene="uvrA"
                     /locus_tag="PLG49_03020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ16319.1"
                     /translation="MVNNKIIIKGAREHNLKNIDLEIPRDQFVVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDLIEGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLFARIGIPHCPQCGKEIKKQTIDQIVDHIMKLPERTKIQLLAPIVRGR
                     KGEHRKVLESLRKAGYVRVRIDGLIYELSEEVNLEKNKKHSIEVVVDRLVVKYNIEKR
                     LTDSIETVMELSKGLLLVDVIDGEELTFSQDFACPDCGISIGEIEPRLFSFNNPFGAC
                     PSCMGLGSKLLIDPELIIPNPNLSILEGAITAPGWNSIDKTGSYARSFFEALSRHYHF
                     DLNTPFKDLDEKIKDIILYGSRGEKIKVEYENARGKGSYVYVFEGIIPNMYRKYSETS
                     SEFMKQEYESFMSNHPCHDCGGARLKPEALAIKLGGKNIAQVADMSVLEVRKFLEDLE
                     LSDMQKAIGKQILKEINARIGFLIDVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSGL
                     VGVIYILDEPSIGLHQRDNHKLIKALTNLRDLGNTLIVVEHDEDTMYAADYIVDIGPL
                     AGSRGGEVVCTGTVEDIKKSLRSETGAYLSGRKKIEIPQVRRKPNGKWLEIKGAKENN
                     LKNIDVKIPLGVFCCVTGVSGSGKSSLINEILYKRLARDLNRAKTKPGRHDKLLGLEH
                     LDKVINIDQSPIGRTPRSNPATYTGVFDMIREVFAQTPEARMRGYTKGRFSFNVRGGR
                     CEACNGDGIIRIEMHFLPDVYIPCEVCQGKRYNRETLEVRYKGKSISDILEMTVEEAL
                     DFFENHERIRRKLETLNAVGLSYIKLGQPSTTLSGGEAQRIKLATELSRKSTGKTVYI
                     LDEPTTGLHFADVHRLIEILQRLTEGGNTVLVIEHNLDVIKTADYIIDLGPEGGDRGG
                     EVIAFGTPEEVAEVSHSYTGQFLKRILRG"
     gene            complement(4788..6773)
                     /gene="uvrB"
                     /locus_tag="PLG49_03025"
     CDS             complement(4788..6773)
                     /gene="uvrB"
                     /locus_tag="PLG49_03025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ16320.1"
                     /translation="MGKFQLVSEYKPTGDQPQAIKILSESIKNNNKFQTLLGVTGSGK
                     TFTMANIIQEVQKPTLIIAHNKTLAAQLYSEFKEFFPNNAVEYFVSYYDYYQPEAYNP
                     QTDTYIEKDSSVNEEIDKLRHSATAALAERRDVIIVASVSCIYGLGDPIDYNTQVLSL
                     RPGMEKSRDDVLRKLVDIQYTRNDIDFSRGTFRVRGDVVEIIPANSSEKAIRVEFFGD
                     EIDRISEIDTLTGEILGLREHISVFPASHYAIPQDKLERAILSIEEELVERVKELKAE
                     DKLLEAQRLAQRTNFDIEMLREVGYCPGIENYSRHLAGRPPGSTPHTLIDYFPDDFLI
                     IIDESHITLPQIRGMYNGDRSRKTTLVEYGFRLPSALDNRPLKFEEFEEKINQILFVS
                     ATPGPYEYEHSSVVAEQIIRPTGLLDPVVDVRPVKGQIDDLYHEINKRVEKNQKVLVT
                     TLTKRMAEELTDYLKEAGIKVNYLHSDIDTLERVEIVRDLRMGVFDVLVGINLLREGL
                     DIPEVSLVAILDADKEGFLRSETSLIQTIGRAARNAEGYVIMYADTITDSMRKAISET
                     NRRRQLQDEYNKKHGIIPKTIEKSIRDVIRATKVLEEEQEYFTEKDPESMDKIELEEL
                     IGKLTKEMKKAASDLNFERAAALRDQLFELKKHLLDL"
     gene            6958..7755
                     /locus_tag="PLG49_03030"
     CDS             6958..7755
                     /locus_tag="PLG49_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16321.1"
                     /translation="MRWYDKLERKLGRFAIPNLMLYIVFLNLAVFIITYLTDLNLYFF
                     LILDPASVMNGEIWRLFTYIFIPPETSHPLFMGFVLYLYYLIGNSLEHEWGTFKFNLF
                     YFLGMLGTTLCAFITGLPVTPHYINLSLFLAFAKIYPDFELLLFFFLPVKVKYLAIIN
                     WFFFIISLILLPLPWKLAVAASLINYFAFFGKDIINTIKTNRKVKKNKQEFRSKLPKE
                     TTRHKCTVCGITEKDDPSMDFRYCTSCDGDYEYCMNHLKNHEHIKNV"
     gene            complement(7786..8880)
                     /locus_tag="PLG49_03035"
     CDS             complement(7786..8880)
                     /locus_tag="PLG49_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOQ16322.1"
                     /translation="MVQSLLTGILVGIGLSIVLIFLGIPMYFSEQMVFLLPIALVLSL
                     FNFRYLCFSYAGGILGIFYFIARGLKSYGFGLPEIHLDIPGIIALVGILHLMEAWLVF
                     AEGSRDSIPIVIKKDDSYAAAYLMQRYWPIPFSLLIMEFVTSIPQGMIETPEWWPIMK
                     NIITPPNPELSLIYTVLPITAILGYGDIAVSQKPEVKSKKTAVSLMFYSSLLIVIAIM
                     SPERLWLQFLGIFLMPLVHEIMIVGNQRKEKISKPLFTFPKKGVRILELKADGVAEKM
                     GMKRGDIILRINGIEIENYAHMKVVLSNYHTFLWFDVLSADGEEKYFEYKAYPYGIND
                     IGIVPLMEQAVKVFRLEDMENVGLFRMLKK"
     gene            complement(9069..10247)
                     /locus_tag="PLG49_03040"
     CDS             complement(9069..10247)
                     /locus_tag="PLG49_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ16323.1"
                     /translation="MKNRKSFISGLIVGMIIIITINTLFFGFKIVLGMSGKANLASSE
                     KLEKILRYLNKYYVDDIDMKDLEEGMYRGIVDGVGDPYTIYLNEKQYNSFMTQTAGRY
                     AGIGVVVSVDQEDQLITVVSPFEGSPGEKAGLVPGDKIIKVNGFDVTGIDLDEAVSMM
                     KGPAGTKVTLTVYRKEEARTFDVEIERANIDYPSVAHEMLDNQIGYIKISSFDEVTYD
                     QFMKALNDLRQQGQKGLIIDLRNNPGGLLSVVADIADEILPEGLIYYTEDKAGHKETC
                     MSDAKELEIPLVILVNENSASASEILAGAVKDHNKGKVVGTQTYGKGLVQTVFPLGDG
                     SALKVTIAKYYTPSGVCIQGIGIEPDVIVELSEETDNTTSEEEDVQLEKAIEVLMSQL
                     "
     gene            complement(10360..11502)
                     /locus_tag="PLG49_03045"
     CDS             complement(10360..11502)
                     /locus_tag="PLG49_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ16324.1"
                     /translation="MRIFIRKGLLLLLSVFFAFSTLYTVQANSLEAKRSQLEEIKKSL
                     KSAKENLEKTKKEKNEVMQNIARLDQELENVEAYLDEINTKIKELEAEIEEKQKELEE
                     AERQRAEKYEEFKERVKCMYMNRKVGYIQVLFNSKSFSDFLNRIDIFSRIAEHDQKLM
                     DSMKENEEKIKTQQQELNDKKDEVTLIKKEQLGVKYTLEQTIKQREAALQELSKDEVL
                     WMQMIKEEEEISKKLEQEIIELTKRSTRVYSGGALEWPVPGYYSLSSGYGERTDPIYG
                     GAEFHKGIDIPAPRGVSVVAAADGEVISAGYINGYGYTVIIDHGSGITTLYGHNSQLL
                     VSTGQIVSRGEAIAKVGSTGNSTGNHCHFEVRIKGAHVNPLQYFSR"
     gene            complement(11526..12425)
                     /gene="ftsX"
                     /locus_tag="PLG49_03050"
     CDS             complement(11526..12425)
                     /gene="ftsX"
                     /locus_tag="PLG49_03050"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOQ16325.1"
                     /translation="MKIRTMKYFAAQGLQGIWRNRLMSVASIATISSCLMIVGIFYCI
                     AVNIDYMLESLEGTAGITVFYTKENYTERDIEVIREKISKISYIKDIRYISPAEALEA
                     EKKEWGEYASLLEGLEDDNPLPPTFELTLEDIRFQKDVISELNKINGIEIRYFEEETK
                     ILISFNNIIRIISLALILILSFIGIMLMDNTIRLTVYVRKTEISIMKYIGATDWFIRW
                     PFIIEGIIIGFIGSIIPLGIIWVSYNFITQLIFEKNTLLQNILSFRTTEEIFRVLTPI
                     SLIIGISIGVIGSSISIRRYLKV"
     gene            complement(12415..13101)
                     /gene="ftsE"
                     /locus_tag="PLG49_03055"
     CDS             complement(12415..13101)
                     /gene="ftsE"
                     /locus_tag="PLG49_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOQ16326.1"
                     /translation="MIELHNVTKTYENGVDALLNVSLKIEKGEFVFIIGTSGSGKSTL
                     IKLLLKEVEPNHGEIIVNNKNITKLKKSKIPYLRRGLGVVFQDFRLLPNKTVYENVAF
                     AMQIVEASPKEIRRNVPMVLSMVGLARKARAYPNQLSGGEQQRTALARAIVNNPPILL
                     ADEPTGNLDPKTSWEIMKVLKDINSRGTTVIVATHDLDIVRKMKRRVIELREGIVVQD
                     LPKGGYSYED"
     gene            complement(13189..14280)
                     /locus_tag="PLG49_03060"
     CDS             complement(13189..14280)
                     /locus_tag="PLG49_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ16327.1"
                     /translation="MISNQVLQSTIDGLKAITRRDFTITDTEAKVLASTEDRQMSLYQ
                     SIIEDFVNSQAESQLIQGYHYFKVFDDNVAEYIVSIKGEDEEAYRIGKIAAFQIQNLL
                     VAYKERYDKDNFIKNLLLDNMLLVDIYSKAKKLNIENNVTRVVYLIDTQPDKDINYVE
                     IVRSIFPAKSKDFVTAVDERSVILVKEITEKEHREDKEKIARMIYDTISGEAMTRVSV
                     AVGTFVEDLKDVSRSYKEAKMALEVGKIFYSEKNIISYDKLGIGRLIYQLPVPLCHMY
                     VNEVLQGRSISEFDEETLTTVNKFFENNLNVSETSRQLYIHRNTLVYRLDKLQKMTGL
                     DLRNFEDAITFKITLMVSKYMNYVEKTSY"
     gene            complement(14431..15543)
                     /gene="ugpC"
                     /locus_tag="PLG49_03065"
     CDS             complement(14431..15543)
                     /gene="ugpC"
                     /locus_tag="PLG49_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOQ16328.1"
                     /translation="MAELKLKNIYKKYPNGFVAVKDFTLDIEDKEFVIFVGPSGCGKS
                     TTLRMIAGLEEISSGELWIGDRLCNDVAPKDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKTPKPEIDRRVKEAAKILDIEHLLDRRPKALSGGQRQRVAMGRAIVREPKVFLMDE
                     PLSNLDAKLRVQMRTEISKLHQRLQTTFIYVTHDQTEAMTLGTRIVVLKDGVIQQVDT
                     PSNLYKYPKNLFVAGFIGSPQMNFIEVKVVKQGEDVLLTFGNEEIKLPQAKAKKLIEG
                     GYVGKEVIMGIRPEDIHDEPAFIEASPTSTISANVEVTEMLGAEVFLYMVTNGQNITA
                     RVDPRSEAKPGDTIKIALDANNIHVFDKDTELTITN"
     gene            complement(15791..16675)
                     /locus_tag="PLG49_03070"
     CDS             complement(15791..16675)
                     /locus_tag="PLG49_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOQ16329.1"
                     /translation="MEKLIQKESFRDYLLIMIGTIILACGINMFFEKQNLVTGGVTGL
                     AIVIKYISSGMFDGGIPLWFTNIVLNVPLYLIGIKIRGRGFGKKTLYAIIFLSFALFF
                     TKYMPAPPTDILLSSLFGGVLSGVGLGFVFIAYGTTGGTDLAANIIQHFIKHISVGKL
                     MMILDGIIIFAGAVIFGAINTMYALIAVYISGKVLDNMLEGVHFSKAVFIISDKAEII
                     ADEIMRNLDRGVTGLNGKGMYTKMEKEILFCVVSKKEIFKLKEIVRNMDSKAFVIVSD
                     AREVLGEGFIEYDAKETL"
     gene            complement(16768..17505)
                     /locus_tag="PLG49_03075"
     CDS             complement(16768..17505)
                     /locus_tag="PLG49_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOQ16330.1"
                     /translation="MNDRINIMDVPIDVVTMDEAVLKLLEFLKSEQSHAVFTPNPEII
                     MEAQNNKDLMQALKRASLVVPDGIGVVLASKILEGPRLPERVAGYDLVQNLFKSAAQT
                     DYTFYFLGGQEGVAQKAAERMAEQYKGLKIVGSQHGYFKDEKEIIKKIQDVKPDVLLV
                     GLGAPKQEIFIDKYKDILPCKLCIGVGGSFDVMAGKVKRAPVIFQKLGLEWFYRLISQ
                     PSRIKRMIRLPIFMWNIVRISAYNKKK"
     gene            complement(17551..18786)
                     /locus_tag="PLG49_03080"
     CDS             complement(17551..18786)
                     /locus_tag="PLG49_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOQ16331.1"
                     /translation="MKLIKNDGNLKQYFIAAAFVIIIFIFGILTFSAQGPSILKSSYT
                     QFKSGLANNFSANVLLKNTINEFEKGVNQNIYGRLVFVDIFGGLQRLMGKNVVIDADA
                     ENSTIYKMKNGQLTFSGYPKDLTETAKNVIELKEYLDGKGIEFLYVQAPHKVSKYEDQ
                     LPVGIEDYNNKKADDMLSLLREGNVRVIDLREEIQKDNLIHEDMFYKTDHHWTTPAAF
                     WGYCKVISYINENLDIPVSNVEVTTNLENYFVEEYKNCFLGSQGRRVGRLYAGLDDYT
                     FIHPDFDTSYTMDYHYSNGKTQTLEGTFDDVFVKKENLDMSQPPSTNRYASYLGGDYP
                     LLVIDNKLLDEGNILIVQDSFGLPFSPFMSLNFSETQIFDLRHYKEKTLVEFIDENNF
                     DLVLFLQNANNYEQNKVGE"
     gene            complement(18822..20237)
                     /locus_tag="PLG49_03085"
     CDS             complement(18822..20237)
                     /locus_tag="PLG49_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOQ16332.1"
                     /translation="MLFSSVVFIFFFLPFVLFMYYVPFRKSIKLKNILLLIASLGFYA
                     WGEPVFVLIMVLSIVFNWLFGLLVDKYREDKVKSRFVLTLMVCFNLTIIFIFKYLMFT
                     LENINFFFKTNIHVPYIALPIGISFFTFQNMSYVIDVYRKNGEVQKNPLYVGLYISFF
                     PQLIAGPIVRYETIAKEINYRKETLDDFSQGVCRFIMGLGKKVLLANNIALIADKAFA
                     LGGDKLSVSFAWLGAIAYAFQIFFDFSGYSDMAIGLGRMFGFHFLENFNYPYISKSIS
                     EFWRRWHISLSTWFRDYVYFPLGGSRVKTKRKLIFNLFVVWALTGLWHGANWTFIFWG
                     LLYFVLIAIEKLIDFQNKFKKLNILKYLYTMLFVLLGWVLFRADSIGQAIDYMKAMFG
                     LKGSPAFDDTTLLYFQEYKYFLLLCVLSSVPLSNWLSAKINKESKLYSIGYICSYLLI
                     FVLSISYIIKGTYNPFIYFNF"
     gene            complement(20382..22295)
                     /locus_tag="PLG49_03090"
     CDS             complement(20382..22295)
                     /locus_tag="PLG49_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ16333.1"
                     /translation="MKKKTFRWITLFLIIQLILAFFSPVYAEESTANNTENNSVQIFV
                     NDVQVELTAAPITKNGTTLIPLRSLLESVKMNVQWNNKEQKITISKKDLLVELKIDST
                     SAQVNGEEVKLSQAPVLEGSIVYVPLRFVSEVVGGSVSWDSVNKVIAIYIPVEEKPLQ
                     TSIVKNGKTIYTNEEIIVKDEWVYIPVNKLAEIIGIKANVSQDLKSVSIVKDKDSLQL
                     QVDSNLAVINQVTVETEFKPFIHNNKLFVPAQFVGDLFGFTVTWNDETKQLFFYSRAS
                     MFDLEIIRKYPSPTNIPKQVPEDSVKPVEGRRLIVSDNPETLDNLAFPYTEAVLWSDR
                     VYTDEDKVDYRTYVYHVNQTGNTIKLGINITNYSDSNEIEIKDLRGSGKESSDGWSII
                     DVGVKIAELSISDKLPDIDLANTVVGKGESITLDYFFANHEHLMSFLHEFTVEKKSGT
                     GPLDFEVRIVASKNKESDLSRIHGPLLKATGVHPRGTWEYSEIYAELPVYKVGDKDYS
                     YSISNGFTDHLFTAEKSLGDKNESRDNKGHFGATYRVKIPIENKDGKPRKLLIRVNPR
                     GGYYAGAISTPEGEYIIPPMNPFYEVCNVMEYTVEEGKDEVELVIMHAGGGHLPVSIN
                     ILTIYTLEDVIKR"
     gene            complement(22313..23395)
                     /gene="csaB"
                     /locus_tag="PLG49_03095"
     CDS             complement(22313..23395)
                     /gene="csaB"
                     /locus_tag="PLG49_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOQ16334.1"
                     /translation="MGSVVLSGYYGFNNAGDEAVLYAIIHSLREKIKDIDITVLSNSP
                     EETAKTYQVKAVDRWSMRQVLQAIKKADLLISGGGSLLQDVTSWKSIGYYLSVMLIAK
                     ICRKKLVLYSQGIGPVNMKFNRILIKWIVNKADYISVRDEESKEELERLGIKKEIEVV
                     PDPVMAMTLSSEEEKGRVLLPNQDNKKRLGVYLRDWKVDSSFYKKVKKVLKWALNEGW
                     EIVFVPMHHPDDIEIAREMSKEIPSSLVYEGDNSPVNILSLTNTMDYILSMRLHGLIM
                     ASVRKVPFIGLSYDPKVDRFVQTLKVGKTINIHDFDENDIIMELKEAHLKKDEMKKRI
                     SVLEKEIQEASKKPANYIAKLLSNQK"
     gene            complement(23383..25317)
                     /locus_tag="PLG49_03100"
     CDS             complement(23383..25317)
                     /locus_tag="PLG49_03100"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOQ16335.1"
                     /translation="MKRFINILPVSIIIISLILSGIQGVKRFSAEQSQKDVQILVTYN
                     DIKKATEIYGKPLEEVLVRLKEAGVGGVLIKEQTIKSDVRGNMVSWEEQGEVTVFSGT
                     ELMLFEKIADTNFSDINPSHYYLWVPDERIRDSIQKHIEYKLGSAPIIEINNQLFIDA
                     GASSNNLFTIGTGYPTDDLEIIANEGFLISPQVRAWPNVTEDALKFVLNDVDNIPNLG
                     TVYFNDVTVPGHGTDTMKDFAKNHKIGIVEFFSEKQKGLYSLIRAGVTEGRYNSIRLH
                     TLADGETSTLTPERVIDRYLLAATERNLKALLVKMPATDQPDKDYEDWYKIVSRIHQA
                     LSNKGYKISSDPEPVHLPSANPVFLWLIGLGPIFLLVLFGRWIDKEKWAWLLAAAAFM
                     AWTGLLFVHPILARQLMALAAAILYPTWAVISCISEKKKTWKELFIAFIKVCLFSFGG
                     ALTIIGLLSESSFVLTIDLFRGAKVAHIFPLLLVPLLIEYKRGNLKMKKIKDLSFSPV
                     TYLALFVLGILGLALIIYVLRTGNEGPKSSYEAMIRNLLNKMLGVRPRTKEFLIGHPL
                     MLVSLYYGYKEKYFPVLLGAVIGQISIVNTYAHIHTPIMISLIRTFHGIWIGFLGGII
                     LIFVLKGLEGLGRRWGLWEV"
     gene            complement(25347..25841)
                     /locus_tag="PLG49_03105"
     CDS             complement(25347..25841)
                     /locus_tag="PLG49_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16336.1"
                     /translation="MKKKHNRSVLMFLLVIVLGVIFGAVTVFGVSPGSNEDPLVSKSY
                     VDSKIDAVMDLIGKLSSNNSNNNSQAGSYSMTYEVVEVPAGKSLIGHQGTEIILRGGK
                     GLAITAELGGLQDMTEGTDISSGQEIPKYHLIIVPRDDGRGVYVLQDAVFMVRGKYDI
                     LPLD"
     gene            complement(25868..28669)
                     /locus_tag="PLG49_03110"
     CDS             complement(25868..28669)
                     /locus_tag="PLG49_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOQ16337.1"
                     /translation="MKQRKRRIASVIVALFIFTTVFFPFITEATTITNVLYKEVHEEE
                     IRKGISYIKDRRLTAVGWLDTYILKVDLTNPDVRIDVLESTNQYNKRETTTDLIKSNG
                     AVAGINGDFFDMSKNPTASLGMVARDGKLISAYNNTNIDKNKWTTFFIDNEGNPFIDY
                     CKIKMLFYNENGVYIGVAGINKITSFKYAVYLDRNSYTSTAEIDSRHPALYKFVVQDG
                     VITYVSKRGETVEIPENGYVIVIDEPTAVQKRAEFKVGQKVFFDLQTSVDLTSIQMAL
                     GGAGKIVDKGTVPVNPGHLVSPNVRGPRTALGISRDGRTLYLIAVDGRGQSVGATHSE
                     MTSLLMEYDIYDAIHLDGGGSTTIAAKPMGKDELSLLNKPSDGSERKVVNGLGVFFDA
                     PAGELAGIKIIPNNHRVFQNMPVSLEVIGYDKNLNPVPIANSAIKWDVDYIQGNFSNN
                     VFVPKSAGEGIITAQVGNVKGQVALTCMGMPKALKAHPETIRLKPGEGVNLWVVGLDE
                     EGYKGNIDLTKMTWKVDPSLGTVINGKFIAANTTGQGIIEGFYGDVKIAVPVAIGEKV
                     TPLESFEGDRLVEWSGYPSTVTGVVDIDTDIKYEGTKSIKMKYSFKNNPNVTQAAYVN
                     FKEPIVILNNAKAIGMWVYGDNSMDWLRGSLKDANGQEFLIDFAKEINWTGWKYVQAT
                     IPSSVTYPISLSKVYTVTLNSAAERENTIYIDHLSCISLPEIDEANVINSTRLKDKHE
                     KSLERVSAKNSFDITVSGLISNSLDEEIKKELVKQMNENASFSIYFGTAVNEMDIKVP
                     YMKFNNNYQVNDIKNARIIQINASNGGIRAANPEQWKWLQNDLDTDKDNLFIIMDKNP
                     QNEAFSDAAEAELFHKLLKELKAKTNKNIFVISSGQSNQVQILEGVRYICVSGSDLSF
                     LRFRLTDSEIAYEFKPIYN"
     gene            complement(28740..30053)
                     /locus_tag="PLG49_03115"
     CDS             complement(28740..30053)
                     /locus_tag="PLG49_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ16338.1"
                     /translation="MSKKNQKKGKYYLVKKFFSIVFLFVFSFLVLASIGTAGYLHFNK
                     DELAENNTAENGEENKGIMDFFNKKSKKVRTNVAIFGVDEDEIRTDVIIVATFNSETK
                     KIDLISVPRDTRVFLTDAMLQDMRSRNSYVPDTCKINEVHAYAGKEKANEYSVKEVER
                     LLGIKIDYYVKMNLEGFRNIVDEMGGVEIDLPRDFYYVDRAGGLYINLKAGPQTLNGA
                     QAEQLVRWRKDNKGGGYGDLGRINTQQLFLQAFAKKALSPKNVVNAPGIIKNLFKYVE
                     TDISINDALSYVKYLDEIKLDNINMTTIPGEARTIGGISYFIHDEEGTRKLVDEIFFS
                     DDKGEEKSESTISSKDARIEVLNGGQVSGLAQRTGDKLKEEGYNVVSIGNYGGTREDA
                     TRIIVNKEGLGEDLKQYFENSKIIVDESITDQDIDIRIILGLSEK"
     gene            complement(30115..30858)
                     /locus_tag="PLG49_03120"
     CDS             complement(30115..30858)
                     /locus_tag="PLG49_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ16339.1"
                     /translation="MVVQFNPKENSLKCVSIPRDLYIDFSDYKFSSIRGSYQVNVSYC
                     KLTEVYQNVTDKEDAVKVIQEIAEKIVGIPIDYYVKVDLDGFKAIVDLVGGIEVEVPK
                     RMVYHDQSQGLHISLESGLQTLDGEKAEQLVRYRSGYVDGDLGRIKMQQEVLKALCKK
                     VLEIRNPIEIFSLVKEGYQYIETDFSLLDCMKYIEYITGLNVSTIFQEGNMVTIPTYG
                     EKINGLWYEMQDKDKTREILEELFNQKDQ"
     gene            complement(30900..31139)
                     /locus_tag="PLG49_03125"
     CDS             complement(30900..31139)
                     /locus_tag="PLG49_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16340.1"
                     /translation="MGNIFENRNHIKIILIMGLMFMIIVSTLIISNMIVKWATKSDYE
                     INFPGGDSSNNLIAATDNSKEEKQEDEGEVKKLLP"
     gene            31376..32116
                     /locus_tag="PLG49_03130"
     CDS             31376..32116
                     /locus_tag="PLG49_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ16341.1"
                     /translation="MDFKNKAVLVTGASRGIGKEIALSFAKKGANLIINSSRSKEQLE
                     AVKREIESLGCQCYSFLGDVSDYQFVASMFENIYKHFEQVDVLINNAGISHIGLFTET
                     TVTQWKRIIDVNLNSVYNCSYFALPPMIKNHRGNIINISSIWGVTGASCEVAYSASKG
                     GVNSFTKALAKELGPSNIRVNAIACGVIDTDMNTWLSPEEKSELLDQIPLMKMGSSQD
                     VANLCIYLASDESKYITGQIIRLDGGMI"
     gene            complement(32096..32788)
                     /locus_tag="PLG49_03135"
     CDS             complement(32096..32788)
                     /locus_tag="PLG49_03135"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16342.1"
                     /translation="MIKNLSKNFGEIVVFDDFSLDIEEGKIYCIMGPSGCGKTTLLRI
                     IANLESYDKGNIDLKGKKISFIFQEARLLPWKTVRGNLELVCKAKEIKKIDQYLEVME
                     LKEYEHSYPKELSGGMKQRVSMIRAFLYPHDILLMDEPFQAIDVKLKEKLYKEVYKLH
                     KEVNNTIIAITHDLEEALFLADKIIILSQKPAKILYQASLDIPRRDRNPDHEVLTKVK
                     KQILKYLYHASV"
     gene            complement(32793..33551)
                     /locus_tag="PLG49_03140"
     CDS             complement(32793..33551)
                     /locus_tag="PLG49_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ16343.1"
                     /translation="MKDFIIRNKKIYLLISIFILLLIWQIASFFLSPLIVPSPVETAK
                     IFLNFLSKKEFWLDIFVSFERGLLGFFLAVIIGTPLGFLMGLKEECEAFFHPLLVLTQ
                     TTPTVSWLILGWLWFGTGDGAAAVFIIFIIVIPFIMVNVMKGTKEVDSSLMEMAQVFN
                     FSLRKKIFYLYIPQIFPYFMAGCSIASGLTWKGVAMAELLTARSGIGAAMGVARINLE
                     IGQVFVWTAVLIFLGHGFSRLFKYIELSVTKKWR"
     gene            complement(33526..34491)
                     /locus_tag="PLG49_03145"
     CDS             complement(33526..34491)
                     /locus_tag="PLG49_03145"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ16344.1"
                     /translation="MKKIQAIFLVFIIVILAAGCSKGKDQKTSIPDKIIIQAPVAPPT
                     SPLVKMVEDKMLGDNVEFILYQNVEEATTRVARGEADFTVLPVNVASKLYNKEADVSL
                     LNVNTWGILYLLSRNGEVKSWEDLRGKELFVGAQGASPDVITRYLLSQNGIGEEEITI
                     SYGTSPEMLQWTIAGKADIVVLPEPLVTQCLLKAENMEISMDYSQEWEKINGKGSRLP
                     QTGIVVRTSFAKEYPDIVEDFQNAYKTALETVVSDPKAVSGLIEEKLEIPAAVFENSM
                     TRTKLEFATAEDAKTDVEAYLKALNELSAEMIGGKLPDEGFYYKK"
     gene            complement(34588..35064)
                     /locus_tag="PLG49_03150"
     CDS             complement(34588..35064)
                     /locus_tag="PLG49_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16345.1"
                     /translation="MSITMSAFSIIFGLFFMVMALGKNTVMEPNPVEKMINNKSGVYD
                     GIITAIEENEQGQRIKIEYEKDGQRAEALFYSVNMPDSIIDFKFNFSVGTKVSFNLSE
                     NGNINLMTIHKKEEAKASATEEKGKIQWGLLFFGMLLFAFGVYSCIQNIYWLLFIT"
     gene            complement(35444..35635)
                     /locus_tag="PLG49_03155"
     CDS             complement(35444..35635)
                     /locus_tag="PLG49_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16346.1"
                     /translation="MDGIISTFGKEIKKVVFGFTPLLKDDYEYKEIYEEDTTLFVKGD
                     IIIDLFEKNHLRFPVLART"
BASE COUNT        11211 a         7225 c         4930 g        12392 t
ORIGIN      
        1 taattctcct tttataactt gaaaaaaaat cactatactt ctataatgat aataaaatga
       61 atagtttttt atcataattg acaggaggat gaataaaatg tttagaagta aaaagcgtct
      121 tttggcattg ggtttaactg ctgttatttc tttatccagc gcaacagctt tatatgcttc
      181 acctgttatt aaaactttac gggcacaata tatgggcatt aaatttagtt ataacggaaa
      241 aattgtagat cctggtaatc aagaaccctt tgttgttgac caaactacat atgttccaat
      301 ccgaatggtt ggagaattag ttggtaaaaa tgtccaatgg gatggtaaca acaaacttat
      361 ttccattaac gatactgtag accaaacgtt gatacaaaaa gatttagaaa ttattcaact
      421 aaaagcagaa attgaatctt taaaacaaca attagaagca gctaaaaaac aaacaccccc
      481 tactcaaact gaagacaaaa acaccagtaa gattagttta tcaaatttag aagatgatat
      541 taacgaagac tatggcgaat taaaaggcgt tgtatttgat atctatctaa gcggtgacag
      601 taaagaaata gaaattgaaa tggaaacaga cttataccgc tataaagatt actgggataa
      661 attaagcgaa aaggatgtag aaaaacatct tgaaaaaata tgtaaggcta tctgggaagt
      721 ttatgacaaa gctaaaattg ttggcgactt atacgatatc gatgaaagag aatatgtctt
      781 agaatttagt acggattcaa gaaaaaaatt aactgttgaa tatgttaaca aattggatct
      841 ttctaaatta gaagatgaat tagataaccg atatgagcat tacctgtcgc cttacatata
      901 cctttccctt gacataaaag gtgatgaaga agaaatcgaa tattatctta acgttgatta
      961 tcgtgaatgg tgggacgact tagatgatga acaagttaaa accttaatgg gaaaaatcta
     1021 tgatgatatt aaaaaggcag cacctaatgc aacaagtatt gaaggatata tctatgacac
     1081 cagcagaaaa tctgtcattg cggaatattg gagaacaaag agtggtaatg ctgtattttt
     1141 accatattat taataaatat gacaatcctt taattaataa aaaaagctct gtgtgcaaac
     1201 acagagcttt tttacactta tgccattcat tttaccttat ttctttttct gtcatcaata
     1261 aacaccaata tagcaataac aagcacgtaa atatctccaa ttaacaacat cgttttacca
     1321 aaggcaaaat tcccttagtg ccaaagacta aattgcaaaa atctaattct tatacaacat
     1381 tataatcctc tgctacaaaa ttcaataatc gaacagtata tctttcctgc cctcaattca
     1441 agtaaaacgc tgaaaattta tatattatat attttataga atattaatag tattaaataa
     1501 accatttgat ttcagtaaat tttaaacatt aaaaaacaaa gtcatctgac acataagaga
     1561 agtactaaaa ataattgacc tgatgattat aatctgtatg ctcattttga acatattcaa
     1621 gtactgcctg aatctccagc atattaggat tttcactttg tttaatcaca atatttttag
     1681 gtcttccttc gtctccccgc atttccaaat agtattttaa aatgtccgct gcaatttcat
     1741 tgctgtagtt accctcataa atcatttcgt attcatcttt aggaagttta agattaatct
     1801 gataacaccc atcttcttgc cgcaccctgg tgtccataat gctattaatc attatcatca
     1861 ccccaccttt gccttttcga ttagtttctg gcaaaggcgg tggtttcatt ctctttttct
     1921 tgtggtaaaa tatggattta tcctctgagg attcttttca aaaactgacc tgtataagaa
     1981 tggctcactt ctgctacttc ttctggtgta ccgaaagcaa tgacttctcc tcccctgtcc
     2041 cctccttcag gacctaaatc aataatataa tctgctgttt ttataacatc taaattgtgt
     2101 tctatgacca gaacagtatt ccctccttct gttaatcgct ggaggatttc tattaaccgg
     2161 tgaacatctg caaaatgaag ccctgttgta ggctcatcca gaatatacac tgtttttcct
     2221 gtactttttc tgcttaattc cgttgccagt tttattcttt gtgcttcccc acctgaaaga
     2281 gtggttgaag gttgtcctaa ttttatataa gataaaccca cagcatttaa agtttcaagt
     2341 tttcttctta ttctttcatg attttcaaaa aagtccaaag cctcttctac tgtcatttcg
     2401 agaatgtcag aaatggattt tcccttataa cgaacttcta aagtttcccg gttataacgt
     2461 ttcccttgac aaacttcaca aggaatatag acatccggca aaaagtgcat ttcaatccgg
     2521 ataatcccgt ccccattaca tgcctcgcaa cgtcctcccc taacattaaa gctaaaacgc
     2581 cctttagtat agcctctcat tctggcttca ggtgtttggg caaagacttc tcttatcatg
     2641 tcaaaaacac ccgtgtaagt tgctgggttt gaccttgggg ttctgcctat tggagactga
     2701 tcaatattaa tcaccttatc taaatgctcc agacctaaaa gcttatcgtg ccgtccgggt
     2761 tttgttttag ccctgtttaa atccctggct aaacgcttat ataaaatttc attaattaaa
     2821 gaactcttac ctgaacctga tacccctgtt acacaacaaa aaacacccag gggaatctta
     2881 acatcgatgt tttttaagtt attttctttt gcccctttta tctccagcca tttcccattg
     2941 ggttttcttc ttacctgagg aatttcaatt tttttacgcc cgcttaaata agcgcctgtt
     3001 tctgacctca aagatttctt aatatcttct acagttccgg tacatactac ttcccctccc
     3061 ctggaacctg ccagaggacc aatatcaacg atataatcag cagcatacat agtatcttcg
     3121 tcatgctcaa ccacaatcag ggtattgccc aagtccctga gattcgttaa agcctttatg
     3181 agcttatggt tgtccctttg atgaagacca atactaggtt cgtccaggat gtaaatcaca
     3241 ccaaccaatc ctgaaccaat ttgagtcgcc agccttattc tttgagcttc tcccccagat
     3301 aaagttccag ctgccctgga aagggttaaa taatccaatc cgacatcaat taaaaagcct
     3361 attctggcat taatttcttt taatatctgt tttcctattg ctttttgcat gtcacttaat
     3421 tctaagtcct ctaaaaattt tcgtacttct aaaaccgaca tatcagctac ctgggcaata
     3481 ttttttcctc ccagcttaat tgccagggct tcaggcttta acctggcccc tccacagtca
     3541 tgacaaggat ggttagacat aaaactttca tattcttgct tcataaattc tgaacttgtc
     3601 tcagaatatt ttctgtacat attcggaata attccttcaa aaacatagac ataagaacct
     3661 ttgcctcttg cattttcata ttctactttt attttttctc ccctggagcc atataaaata
     3721 atatctttaa ttttttcatc caaatcttta aaaggtgtat ttaagtcaaa gtgataatgc
     3781 ctcgataaag cttcaaaaaa acttcgggca taactgccgg tcttatcgat tgaattccag
     3841 ccgggagcag taatggctcc ttctaaaatg cttaaattag gattaggaat aattagttcc
     3901 ggatcaatca aaagcttgct gcccaaaccc atacaagacg gacaagctcc aaaaggatta
     3961 ttaaaggaga acaatcttgg ttcaatctct ccgatactga taccacaatc agggcaggca
     4021 aaatcctggc taaaagttag ttcttcccca tcaatcacat caaccaaaag caagcctttt
     4081 gaaagttcca tgaccgtttc aatagaatct gttaaacgct tttcaatatt atattttaca
     4141 actaatctgt ctaccacaac ttcaatggaa tgtttcttgt ttttttcaag attaacctct
     4201 tcagataatt catagataag cccatctatt ctgactctga catatcctgc tttacgtaag
     4261 ctttccagaa cctttctgtg ttcacctttc ctgccccgta ctattggagc cagtaactgt
     4321 atttttgttc tttccggtaa tttcatgata tgatcgacaa tttggtcaat ggtttgcttt
     4381 tttatctcct taccacattg agggcagtga ggaataccta ttctggcaaa taataacctg
     4441 agataatcat atatttcagt gactgttcca acagtagatc ttggatttct gctggttgtt
     4501 ttttgatcga tggatatagc aggagaaagg ccttcgatta aatcaacatc cggcttttcc
     4561 atttggccta aaaattgtct ggcatatgaa gagagagatt ccacatatct tctctgacct
     4621 tctgcataaa tggtgtcgaa agctaaagaa gatttaccag aaccacttaa gccggtaaag
     4681 actacaaatt gatctcttgg tatttccaaa tctatatttt ttaaattatg ttctcttgca
     4741 ccttttatga taattttatt gttgaccatg ttaacctcct gactgcatta taaatccaat
     4801 aaatgcttct tcaattcaaa taattggtcc ctgagagcag ctgcccgttc aaaatttaaa
     4861 tcagaagcag cttttttcat ctctttcgtt agttttccaa ttaattcttc taattctatc
     4921 ttgtccatgg attccggatc tttttcggtg aaatattctt gttcttcttc taataccttt
     4981 gttgcccgaa tgacatcccg aatacttttt tcaatcgttt ttggtatgat tccatgtttc
     5041 ttattatatt catcttgaag ctgtcgtctt ctattggttt ctgatatggc ttttctcatc
     5101 gaatcagtta tggtatcagc atacataatc acgtagcctt ctgcatttct tgcagctctt
     5161 ccaatggttt gaattaagga tgtttctgag cgtaaaaaac cttccttgtc ggcatctaat
     5221 attgccacaa gggatacttc tggaatatca agcccttctc ttaaaagatt aattcccact
     5281 aaaacatcaa aaacacccat tctcaaatct ctgacaattt ctacccgctc cagggtatca
     5341 atatctgaat gcagataatt gactttaatt cctgcctcct tcaaataatc ggttaattct
     5401 tcagccattc tttttgttaa cgtcgtcact aaaacctttt gattcttttc tactctttta
     5461 ttaatttcat ggtataagtc atcaatttgt ccttttacag gccgaacatc tactacaggg
     5521 tccaataagc cagtaggcct tataatttgc tctgctacca cagaagaatg ttcatattca
     5581 tacggacctg gcgttgcgga tacaaataat atctgattga tcttttcttc aaattcttca
     5641 aattttaatg gacggttatc caaggctgaa ggcaatctga aaccatactc cactaaagtc
     5701 gtctttcttg atctatcccc attatacatt cctcttatct gaggaagcgt aatatgggac
     5761 tcgtctataa taattaaaaa atcgtcagga aaataatcaa tcaaggtatg cggcgtactg
     5821 cctggtggcc gtcctgcaag atgtctcgaa tagttttcta ttccagggca atagcctact
     5881 tctcttaaca tttctatatc aaagtttgtt ctttgtgcca gtctctgtgc ttctaaaagc
     5941 ttatcttctg cttttaattc ttttactctt tccactaatt cttcttcaat ggacaaaatg
     6001 gctctttcca gtttatcctg agggatggca tagtgagaag cagggaaaac ggatatatgt
     6061 tctcttaacc ccagtatctc accagttaag gtatcaattt ctgaaatcct gtcaatttca
     6121 tctccaaaaa attcaactct tatggctttt tctgatgaat tggcagggat gatttccaca
     6181 acatctcctc ttacccggaa tgttcctctg ctaaaatcta tatcatttct tgtatattga
     6241 atgtcaacca gctttcttaa gacatcatcc cgactttttt ccatccctgg tcttaaggaa
     6301 agaacctgag tattataatc aatggggtcc cccaaaccat agatacagga cacacttgct
     6361 acaataatga catcccgtct ttctgccagg gctgctgtag cagagtgtct taatttatca
     6421 atttcttcgt ttactgaaga atctttttca atataagtat ctgtttgagg attataggct
     6481 tctggttgat aatagtcgta gtaacttaca aaatattcta cagcattgtt tggaaaaaac
     6541 tctttaaatt cactgtataa ttgtgctgcc agagttttat tatgtgctat aattaaagtt
     6601 ggcttttgaa cctcctgaat aatgtttgcc atggtaaaag tttttcctga gcccgttacg
     6661 cctaaaagag tctgaaattt attattattt tttatggatt ctgaaagtat ctttatggct
     6721 tgtggttgat ctcctgtagg tttatattct gatacgagct gaaattttcc catactatac
     6781 ctactctctt taattaataa tcattcttaa caattatata attataacat atgttttgtc
     6841 ctttgaaaac atatgtttgc aaaaaccaaa aaattcttga ccttcctata aaaataagtt
     6901 ataaaaagaa tggtaaagtt tttacattta tattttcatt tataaaggag aatgactatg
     6961 cgatggtatg acaagcttga acgtaaactc ggtcgttttg caattccaaa tttaatgctc
     7021 tatatcgtat ttttaaatct tgctgtcttt attattactt atcttaccga cttgaattta
     7081 tatttttttc ttatacttga tcctgcttcc gttatgaatg gtgaaatttg gagacttttt
     7141 acttatattt ttattcctcc tgaaacttct catccgcttt ttatgggctt tgtattgtat
     7201 ctgtattatc ttataggcaa ttctttggaa catgaatggg ggactttcaa atttaatctt
     7261 ttttattttt taggcatgct gggaactacc ctttgtgcct ttataaccgg actccctgta
     7321 actccgcact atatcaactt atcccttttt cttgctttcg caaaaatata tcctgatttt
     7381 gagctgttac ttttcttctt cttgccagta aaagttaaat atctcgcaat aattaattgg
     7441 ttctttttta taatttcact tatcctattg cccttaccat ggaaactggc cgtcgctgct
     7501 tcgctcatta attactttgc tttctttggc aaagatatta taaacacgat aaaaacaaac
     7561 cgaaaagtca aaaaaaataa acaagaattc aggtcaaagc tacctaaaga aactacccgc
     7621 cataaatgta cagtttgtgg aataacggaa aaagatgatc ccagcatgga tttcagatat
     7681 tgcaccagtt gtgatggaga ctatgaatat tgcatgaatc accttaaaaa tcatgaacac
     7741 ataaaaaacg tataaaaaac atataaaaaa tagaaccaaa aggttctatt tttttaacat
     7801 ccggaataat ccaacattct ccatgtcttc caatcggaaa acttttaccg cttgttccat
     7861 taaaggtaca attcctatgt cattaatacc atatggataa gctttatatt cgaaatactt
     7921 ttcttcccca tctgcagaaa gtacgtcaaa ccataaaaag gtatggtaat tacttaatac
     7981 caccttcata tgagcatagt tttctatttc tattccatta atcctgagga ttatatctcc
     8041 cctcttcata cccatttttt cggcaacccc atctgctttt aattcaagaa tccgcacacc
     8101 ttttttggga aaagtaaata aaggcttaga aattttctct tttctttggt taccaacaat
     8161 catgatttca tgaactaagg gcattaaaaa aatacctaaa aattgaagcc ataatctttc
     8221 tggtgacata atggcaataa caattaataa tgagctgtag aacattaatg atacggccgt
     8281 cttttttgat tttacttctg gcttttgtga tacagcaata tccccgtaac ctaaaattgc
     8341 tgtaatcggt aaaacggtat aaattaaact cagttccgga ttaggaggag taatgatatt
     8401 tttcattatg ggccaccact ctggcgtctc aatcataccc tgaggaatgg atgtcacaaa
     8461 ctccataatc agcaaagaaa aaggaatggg ccaatatctt tgcatcaaat aggcagcagc
     8521 ataactatca tcctttttta tcacaatagg aatactgtcc cggcttcctt ctgcaaagac
     8581 aagccaggcc tccataaggt gaaggatgcc tacaagagct ataataccgg gaatatctaa
     8641 atggatttcc ggtaaaccaa atccataact ttttaatccc ctggcaataa aatagaaaat
     8701 tcccaggatc cctccggcat aagaaaaaca taaataccta aaattaaaca atgaaaggac
     8761 taaggcaatc ggtaaaagaa aaaccatttg ctcagaaaaa tacattggaa tacccaggaa
     8821 tattaagaca atactaagcc caatccctac caaaatgcca gtcaaaagcg attgaaccat
     8881 tttttcaaaa atcttgcctt ttacaaacaa ggctgtttgc agtgccagat aataattttt
     8941 ttatacatcc aataaactaa atatagaatc agtaagaacc aaggttgaaa aaccgccaga
     9001 gaaactgact gcaggctagt tactactatc tgtaaaaaat attgcatata acccacctat
     9061 caaaataact ataattgact cattaatact tctattgctt tttctaattg tacatcctct
     9121 tcttcactgg ttgtgttgtc tgtttcctcg gataactcta caataacatc aggttcaata
     9181 cctattcctt gtatacatac accactgggc gtataatatt tggcaatagt caccttcaat
     9241 gcagacccgt ctcccaatgg aaatacggtt tgaaccaggc cttttccata agtttgtgtc
     9301 ccaacaactt ttcccttatt atggtctttc accgctcctg caagaatttc tgaagcactg
     9361 gcactatttt cattaacaag aatgactaaa ggtatttcaa gttcttttgc atcggacata
     9421 caagtttctt tatgtccagc cttgtcttcc gtataatata tcaggccttc cggaaggatt
     9481 tcatccgcaa tatctgcaac cactgaaagc aacccgccag gattatttct cagatcaata
     9541 attaagcctt tttgcccttg ttgtcttaaa tcatttaaag ctttcataaa ttgatcatac
     9601 gtcacttcat cgaaggatga aatctttata taacctattt gattatctag catttcatga
     9661 gcaactgaag gatagtcaat atttgctctt tcaatttcta catcaaaagt ccttgcttct
     9721 tcttttctgt aaactgtcaa tgtaaccttt gtaccagcag gtcctttcat catggataca
     9781 gcctcatcta aatcaattcc tgttacatca aaaccattca cttttatgat tttgtctcct
     9841 ggtaccaatc ctgctttttc tccaggagat ccttcaaatg gtgaaacaac cgttattaac
     9901 tggtcttcct gatcaacaga aaccacaaca cctatcccgg cataccgtcc tgcagtttgt
     9961 gtcataaaac tattgtactg cttctcattt aaataaatcg tataggggtc cccgactccg
    10021 tcaacaatac ctctatacat tccttcctca aggtctttca tatctatatc atcaacatag
    10081 tatttattta aatatctcag gattttctca agtttctcgc ttgaagctaa gtttgctttt
    10141 cctgacatgc ctaaaacaat tttaaaacca aaaaataatg tattaattgt aataattata
    10201 atcataccga caattaagcc gctgataaat gattttctat ttttcattaa caggcacccc
    10261 tttagtgatt atattttatt gtattaaaat ttactcttta ttataccata caataaaaga
    10321 tgaaggtatt tatatgaaaa aaataaagga cgatgtcctt tatcttgaaa aatattgtaa
    10381 gggattaaca tgagcacctt ttattcttac ttcaaaatga cagtggtttc ctgtggaatt
    10441 acctgtactt cctacttttg ctattgcttc tccccgactc actatttggc cggtagaaac
    10501 caataattgc gagttatgtc catacaaagt tgtaatccca ctaccatgat caataatgac
    10561 cgtatatcca taaccattaa tatagccggc actaatgact tcaccatcgg ctgctgcaac
    10621 aacactcact cccctgggtg cagggatatc aataccttta tggaactctg ctccaccata
    10681 tatagggtct gttctttctc cataacctga actaagagaa taatacccag gaaccggcca
    10741 ttccaatgct cctccactgt aaaccctagt acttcttttc gttaattcta tgatttcctg
    10801 ttctaatttc ttagaaattt cttcttcttc ttttatcatc tgcatccaaa gtacttcatc
    10861 tttacttaat tcctgtaaag cagcttctct ctgttttatc gtctgctcta aagtatattt
    10921 aacacccaac tgttctttct taattaaagt cacttcatct ttcttatcat ttaattcttg
    10981 ctgctgagtc ttgatttttt cctcattttc tttcatgctg tccattagtt tttgatcatg
    11041 ttcagcaatc ctggaaaaga tatcaatccg attcagaaaa tcagaaaaac tcttcgaatt
    11101 aaaaagtact tgaatataac ccacctttct attcatatac atacatttaa ctctctcttt
    11161 aaattcttcg tatttctctg cccgctgtcg ttctgcttct tcaagttctt tttgtttttc
    11221 ttcaatttca gcttctaatt cttttatctt agtattgatt tcatctaagt aggcttctac
    11281 attttctaat tcctgatcga gtcttgcaat gttttgcatc acttcgtttt tttctttttt
    11341 cgttttttct aagttttcct tggctgattt caggctcttt ttaatttcct ccaactgact
    11401 tcgcttagct tctaaactgt ttgcctgaac agtgtaaaga gttgaaaagg caaagaaaac
    11461 agataatagt agaagcagac cctttctaat aaatattctc atcgttcccc ctccaaacaa
    11521 gcaagctata ctttcagata tcttctgata gaaatactac tgccaattac gccaatagaa
    11581 attcctataa ttaaagaaat cggggtcaaa actctgaaga tttcttccgt tgtcctgaag
    11641 gataaaatat tttgaagaag cgtattcttt tcaaaaatca attgggtaat aaaattataa
    11701 gatacccaaa taattccgag gggtataatt gaacctataa aaccaatgat aattccttca
    11761 ataataaaag gccatcttat aaaccaatcg gttgccccta tatatttcat aatactaatc
    11821 tctgtctttc taacatacac tgtaagccgg atggtattat ccatcagcat tattccaata
    11881 aaagataaaa ttaatataag tgcaagacta ataattctta taatattgtt gaaactaatc
    11941 aggattttgg tctcttcttc aaaatatctg atctctattc cattgatttt atttaattca
    12001 ctaatgacat ctttctggaa tcttatatct tctaaagtaa gctcaaaagt agggggtaaa
    12061 ggattatcat cttctaagcc ttccaataat gatgcatact ctccccattc ttttttttct
    12121 gcttctaaag cttctgcagg cgaaatatat ctaatatctt tgatataact gatcttacta
    12181 atcttttccc tgataacctc tatatctctt tctgtataat tctctttcgt ataaaaaaca
    12241 gttattcctg cagttccttc taagctctct aacatataat caatatttac ggcaatgcaa
    12301 taaaagattc ctacaatcat taaacaagaa gaaatagtag caatagaagc aactgacatc
    12361 aagcggtttc tccaaatccc ttgtaaaccc tgtgctgcga aatacttcat cgttctaatc
    12421 ttcatagcta tatccacctt ttggcaagtc ttgaacaact attccttctc ttaattctat
    12481 aactcttctc ttcatttttc taacgatatc caaatcatgg gttgctacaa tgactgtcgt
    12541 tcccctgcta ttaatatcct ttaatacctt catgatttcc catgacgttt ttggatccaa
    12601 atttcctgtt ggttcatcgg ctaaaagaat agggggatta ttgacaatgg ctcttgccaa
    12661 agctgttctt tgctgttccc ctccagacaa ttgattcgga taggcccgag cttttcttgc
    12721 taaacctacc atcgataaaa ccattggaac atttcttctt atttcttttg gagaagcctc
    12781 tacaatttgc attgcaaaag ctacattttc ataaacagtc ttattaggaa gtaatctaaa
    12841 atcctgaaag acaactccca aaccgcgtcg taaatatgga atcttacttt tctttagctt
    12901 agttatattt ttattattta caattatttc tccgtgattg ggttccactt cctttaataa
    12961 tagttttatc aaagtggatt ttcctgaacc actggtacca ataataaaga cgaattctcc
    13021 cttttctatt tttagggaaa catttaataa agcatcgaca ccattttcat aagtttttgt
    13081 cacattatgc aattcaatca agctaatccc ccctgtaagg gcataaaatt tcttatattt
    13141 tacattattt ctattgaaat atcaatatac cttaatatta gaatcttgtt aataggacgt
    13201 cttttctaca taattcatgt atttactaac cattaaagta attttaaaag taatggcatc
    13261 ttcaaaattc cttaaatcaa gccctgtcat tttttgaagt ttatccaagc ggtaaaccaa
    13321 agtatttcta tgaatatata attgtcttga tgtttcagaa acatttaaat tattctcaaa
    13381 gaacttattc actgtcgtca atgtctcttc gtcaaattca ctgatgcttc ttccttgaag
    13441 aacttcattg acatacatat gacacaaagg taccggaagt tgataaatca gtcttcctat
    13501 gcctaattta tcataactga taatattttt ttcactgtag aatattttcc caacttctaa
    13561 agccattttg gcctctttat acgatctgga aacatccttt aaatcttcaa caaatgtgcc
    13621 aacagcaaca gaaaccctgg tcatcgcttc accactaatg gtatcataaa tcattcgggc
    13681 tatcttttct ttatcttctc tatgttcttt ttccgtaatt tcttttacta aaatgacgct
    13741 tctttcatct acagccgtta caaaatcttt tgatttagct gggaaaatac ttcttacaat
    13801 ttcaacatag tttatatctt tatcaggctg ggtatcaatt aaataaacga ctcttgtaac
    13861 attattttca atattgagtt ttttagcttt actgtaaatg tctaccaaaa gcatattgtc
    13921 taaaagaaga tttttgataa aattatcttt atcgtatctt tctttataag caacaagtaa
    13981 attctgaatt tggaaagctg caattttacc tatcctgtaa gcttcctcat cttccccttt
    14041 gatagaaaca atatattctg ctacattgtc atcaaatact ttaaaataat ggtaaccttg
    14101 aatcaactga ctctctgcct gtgagtttac aaaatcctca ataatggatt gatataagct
    14161 catctgcctg tcttcagttg atgccaaaac ttttgcctca gtatccgtta ttgtaaagtc
    14221 tcttctggtt atagctttca acccatcaat ggtactttgt aatacttgat ttgaaatcat
    14281 attatcaaca ctcccgggat tttttagata aatgtgagga tacaccctct ctattttata
    14341 ttatagtata atttgaaaaa aataaagaga aaatacacat attttgcgta tcttctctta
    14401 taattcttca ttttattctt ttatttgaaa ttaattggta attgttaact ctgtatcctt
    14461 atcaaataca tgaatgttat ttgcatctaa tgcaatttta attgtatcac ctggttttgc
    14521 ttcacttctt gggtcaacac gtgctgttat attttgacca ttagtaacca tataaaggaa
    14581 tacttctgct ccaagcattt ctgtaacttc aacatttgca ctgattgtgc ttgtaggtga
    14641 tgcttcaatg aaggctggtt catcatgtat atcttcagga cggattccca taataacttc
    14701 ttttccaaca tatccgcctt caataagttt cttagctttt gcctgaggca atttaatttc
    14761 ttcatttcca aaggtaagaa gaacatcttc cccttgtttt actactttta cttcaatgaa
    14821 gttcatttgt ggtgaaccaa tgaaacctgc aacgaataaa ttcttaggat atttataaag
    14881 attacttggt gtatccactt gttgaataac cccgtctttt aatacaacaa ttcttgttcc
    14941 cagggtcatt gcttctgttt ggtcatgggt aacataaatg aatgttgttt gaagtctttg
    15001 atgaagctta gaaatttctg ttctcatctg aactcttaac ttagcatcga ggttagataa
    15061 aggttcgtcc attaagaata ctttaggttc acgaacgatt gcacgtccca ttgcaacacg
    15121 ttgtctttga ccaccagata aagcttttgg tcttctatct aataaatgtt caatgtcaag
    15181 tatttttgct gcttctttaa cgcgtctgtc aatttctggt tttggtgttt tacgaagctt
    15241 taatccaaat gccatattat catatacaga catatgtgga tacaaagcat agttttggaa
    15301 taccattgca atgtctctat cttttggtgc aacatcatta cataatctat caccaatcca
    15361 aagttctcca gagcttattt cttcaagtcc ggcaatcatg cgaagtgttg ttgattttcc
    15421 gcatcctgat ggtccaacga aaataacaaa ttctttatcc tctatatcga gagtaaagtc
    15481 tttaaccgct acaaaaccat taggatattt tttataaatg ttctttaatt ttaattctgc
    15541 cattaaaaaa agccccctta taaatgagta attagtttct cgcatctaga attaatatac
    15601 tatatttcga taagttgtac tattgctctt ttcaacaaat cacttaaaat agttttgtta
    15661 actttgcata ggctcatgac aagaaagtag ctttttagtc atcatatctc atttattttc
    15721 aaggactttt tataattttt acataatgaa ttactattat ttggaattgt ccaatgtgtt
    15781 ttgtgaaaaa ttacaaagtt tctttagcat catattcaat aaagccttct cccagcactt
    15841 ccctggcatc tgacacaata acaaaagcct tggaatccat atttcttacg atttctttta
    15901 gcttaaatat ttctttttta gaaactacgc aaaacaaaat ttctttttcc atttttgtat
    15961 acattccttt tccatttaaa ccggttacac ctctgtctaa gtttctcatg atttcatctg
    16021 caattatttc tgctttgtct gaaataataa agacggcttt tgaaaaatgc accccttcca
    16081 gcatattgtc caatactttt cctgaaatat atacagcaat caaggcatac atggtattaa
    16141 tggctccaaa aataaccgct ccggcaaaaa tgataatgcc atctaatatc atcatcaact
    16201 tgcctacaga aatatgtttg ataaaatgct gtatgatatt tgcagccaga tctgttcccc
    16261 ctgtcgttcc ataggcaata aatacaaagc ctaatcctac acccgataag actcctccaa
    16321 acaaagaaga taataatata tctgtaggag gtgcaggcat atattttgta aaaaacaatg
    16381 caaatgataa aaatataatg gcataaagag tcttcttccc aaatcccctt cctcttattt
    16441 taataccaat taggtacaaa ggtacattca aaacgatatt ggtaaaccat aaggggattc
    16501 ctccatcgaa catgccactg ctaatgtact taataacaat ggctaatcct gttacgcctc
    16561 ctgttactaa attttgtttt tcaaaaaaca tattgattcc gcaggctaaa ataattgttc
    16621 caatcattat aagtaaataa tcacgaaaac tttctttttg aattaatttt tccataataa
    16681 ttccttccta gtaaaaatat gtcaaaaatt tcttaagcca tgaaagaatt gataaaaata
    16741 aaataataaa aagaaataat ttttaaatta tttcttttta ttataagcag atatcctaac
    16801 tatattccac ataaatatag gaagtcttat catcctctta attcttgagg gctggcttat
    16861 cagtctataa aaccattcta aacctaattt ttgaaaaatg actggtgctc ttttcacttt
    16921 accagccata acgtcaaaac tgcctccaac gccaatacat aatttgcagg gtaaaatatc
    16981 cttatattta tctataaata tttcttgttt tggtgctccc agtccaacca ataaaacatc
    17041 tggcttaaca tcctgtatct ttttaataat ttctttctcg tctttaaaat aaccatgctg
    17101 gctaccaaca atttttaagc ctttatattg ttctgccatc ctttcggctg ctttttgtgc
    17161 aacaccttcc tgtccgccta aaaaataaaa agtatagtct gtttgagcag cggatttaaa
    17221 taaattttga actaaatcat atccagctac cctttcagga agacgtggtc cctctaatat
    17281 ttttgaagcc aatacaacgc caattccatc aggtactact aaagatgccc tttttagagc
    17341 ttgcattaaa tctttattat tttgggcttc cataatgatt tcaggattgg gcgtaaatac
    17401 agcatgactt tgttcacttt ttaaaaattc caaaagtttc aaaacagcct catccattgt
    17461 tactacatca ataggaacat ccatgatatt tattctgtca ttcataagct ggcctccaca
    17521 caataaagta ctaattatca ccgaattata ttattctcca actttatttt gttcataatt
    17581 attggcattc tgaaggaata agactaaatc aaaattgttc tcatctataa attctactaa
    17641 agtcttctcc ttataatgcc ttaaatcaaa gatctgtgtt tcagaaaaat ttaaagacat
    17701 aaagggtgaa aaaggtaaac caaatgaatc ctgaacaatt aatatgttac cctcgtcaag
    17761 caatttgtta tcaataacca acaagggata atctcctcct aaatagcttg catagcggtt
    17821 tgttgaagga ggttgtgaca tatccagatt ctctttttta acaaatacat catcaaaagt
    17881 accttctaaa gtttgtgttt tgccattgct gtaatgatag tccattgtat aacttgtatc
    17941 aaaatcagga tgaataaagg tataatcatc taatcctgca tatagtctgc ccaccctgcg
    18001 tccctgagaa cctaaaaaac aatttttata ttcttccaca aaataatttt ccagattcgt
    18061 tgttacttcc acattggata ctgggatatc caaattttca ttaatgtaac tgatgacttt
    18121 gcaatagccc caaaaggcgg caggtgttgt ccagtgatgg tcggttttat aaaacatatc
    18181 ttcatgaata agattatctt tttgaatctc ttctcttaaa tcaatcactc tgacattgcc
    18241 ttctcttaat aaagaaagca tatcatccgc ctttttattg ttataatctt ctataccaac
    18301 aggaagttga tcctcatatt tcgaaacctt atgaggtgcc tgaacgtaaa gaaactctat
    18361 ccctttccca tcaagatatt cttttaattc aataacattt ttcgctgttt cagtaaggtc
    18421 tttgggataa cctgaaaaag taagttgtcc gttcttcatt ttatagatcg tgctgttttc
    18481 ggcatcggca tcaatcacca catttttacc catcagtctt tgaagcccgc caaaaatgtc
    18541 cacaaagact aatctcccat aaatattctg attaacgcct ttttcaaatt cattaatcgt
    18601 attttttaat agtacatttg ctgaaaaatt attggcaagt ccacttttaa actgcgtata
    18661 agaactcttt aagatactag gaccttgagc agaaaaagtt aaaattccaa aaataaaaat
    18721 tataatcaca aatgctgcag caatgaaata ttgcttcaag ttcccatcat tttttattaa
    18781 tttcatttta gccatcccct catatatgta caggttaatt attagaaatt aaaatatata
    18841 aatggattat aagtaccttt gataatataa gaaattgata aaacaaaaat caataagtaa
    18901 gaacatatat agcctatgga atataattta ctttccttat taatcttagc agataaccaa
    18961 ttgcttaatg gaaccgaact aagtacgcaa agcagtaaaa agtatttgta ttcctggaaa
    19021 taaagtaaag ttgtatcatc aaaagcagga gaacccttta agccaaacat tgctttcata
    19081 taatctatgg cttgtccaat actgtcagct ctgaataata cccatcctaa taaaacaaaa
    19141 agcatggtat aaagatattt tagtatatta agttttttga acttgttttg aaaatcaata
    19201 agtttttcaa tagcaatgag tacaaaatat aatagtcccc aaaaaataaa ggtccagttt
    19261 gctccatgcc ataaacctgt taaagcccat accacaaaca agttaaatat taattttctt
    19321 tttgttttta cccggcttcc accaagcggg aaataaacat aatccctgaa ccaggtagaa
    19381 agtgaaatat gccatctgcg ccaaaattcc gatatggatt ttgatatata tggataatta
    19441 aagttttcta agaaatggaa gccaaacatt cttcccagac ctatcgccat atcagaataa
    19501 ccactaaagt caaaaaagat ctggaatgca taggcaattg cacctaacca ggcaaaactc
    19561 acagaaagct tgtctcctcc caaagcaaaa gccttatcgg ctatcagtgc tatgttattg
    19621 gcaagaagaa cttttttccc taaacccata ataaacctgc atacaccttg cgaaaaatca
    19681 tctaacgttt cttttctata attaatttct tttgctatgg tctcataacg cacaatagga
    19741 cctgcaatca gttgtggaaa aaaagaaata tacaagccaa catataaagg atttttttgt
    19801 acttctccat tttttctgta tacatcaatg acataggaca tattttggaa tgtaaaaaat
    19861 gatattccta tgggtaaagc aatatatgga acatgaatat tcgttttgaa gaaaaaattg
    19921 atattctcta aagtaaacat taaatactta aaaataaaaa ttatcgttaa gttaaaacac
    19981 accattaaag taagcacaaa tctggattta actttatctt cccgatattt gtccaccagt
    20041 aagccaaaaa gccaattaaa aacaatggat aaaaccataa taagaacaaa aaccggttct
    20101 ccccatgcat aaaatcctaa acttgcaata agtaacagta tattttttaa ttttatactt
    20161 tttctaaatg gaacataata cataaataaa acgaacggta aaaaaaagaa aataaaaaca
    20221 acgctggaaa ataacatttt ttcacttcct tatgtcaata taaattccaa gatattttat
    20281 caaaatttat agcaaaaatc cattcttttc aatgcaaata tataaggtta tggaaaattg
    20341 ttttcccata acctcatcaa ccataaatat caaatcaatt cttatctttt gataacatct
    20401 tcaagggtat atatggtcag aatattaata gatacaggaa ggtgtcctcc accggcatgc
    20461 ataatcacca gttcaacttc atcttttcct tcttcaaccg tatattccat cacattacat
    20521 acttcataga atggattcat aggaggaata atgtattctc cttcaggagt actgattgct
    20581 cctgcatagt aacctcctct tgggtttact ctgataagaa gttttctggg cttgccatct
    20641 ttattttcaa ttgggatttt gactctgtat gttgcaccaa aatgcccttt attgtccctg
    20701 gattcattct tatctccaag acttttttct gctgtaaata aatggtccgt aaaaccattg
    20761 gaaatgctat aagaataatc cttatcacct actttatata caggcaactc tgcataaatt
    20821 tctgagtact cccaggtacc tctgggatga acgcctgttg cttttaataa aggaccatga
    20881 atcctggaca agtcgctttc tttattttta cttgcaacga ttcttacttc aaaatctaat
    20941 ggacctgtac cacttttctt ttctacagtg aattcatgca gaaaactcat taaatgttca
    21001 tggttagcaa aaaagtagtc caatgtaata ctttcccctt ttccaacgac tgtattagct
    21061 aaatcaatat ctggaagctt gtcactgata cttaattctg ctattttaac acctacgtct
    21121 ataattgacc aaccgtctga actctcttta ccacttcccc gtaaatcttt tatttctatc
    21181 tcattactgt ccgaataatt cgtaatatta attcccaatt taatcgtatt gcctgtctga
    21241 ttcacatgat agacatatgt acggtagtca actttgtctt catccgtata aaccctgtca
    21301 gaccataaca ccgcttctgt atacggaaag gcaagattat ctaaagtttc cgggttatca
    21361 cttacaatca atcttctgcc ttctacaggt tttacactat cttccggtac ctgtttcgga
    21421 atattggttg gtgaaggata tttcctaata atttctaagt caaacatgga agccctggaa
    21481 tagaaaaaca attgttttgt ttcatcgttc caggttactg taaaaccaaa aagatctccc
    21541 acaaattggg caggaacaaa aagcttatta ttatgtataa agggtttaaa ttctgtctca
    21601 acagttacct ggttaatcac tgccaaatta gaatccactt gcaattgtaa tgaatcttta
    21661 tcttttacaa tagatacgct ttttaagtct tgtgaaacat tcgccttaat accgattatt
    21721 tcagccaatt tattaacggg tatatatacc cattcatctt tcactataat ttcttcgttt
    21781 gtataaatgg tctttccatt tttcacaata ctggtctgta gaggtttttc ttcaactgga
    21841 atgtagatag caataacttt gtttacactg tcccaggaaa cacttccgcc aacaacttcg
    21901 cttacaaaac gtaatggaac atatactatg cttccttcta atacaggcgc ctgacttaat
    21961 ttcacctctt caccatttac ctgggcagaa gtcgaatcaa tttttaactc aacaagcaaa
    22021 tcttttttac tgatagtgat tttttgctcc ttattattcc attgaacatt catttttaca
    22081 ctttccagta agcttctaag aggtattaaa gttgtaccat ttttcgttat aggcgctgct
    22141 gttaattcta cctgcacatc attaacaaat atctgtaccg aattattttc agtgttattt
    22201 gctgtgcttt cttctgcata aaccggtgaa aaaaatgcta aaatcaattg gatgattaaa
    22261 aataaagtta tccatctgaa cgtcttcttc ttcatatttt ccctccatag atctattttt
    22321 gattactaag tagctttgct atataatttg ctggcttttt gctcgcttct tgaatttctt
    22381 tctcaagaac agatattctt ttcttcattt catctttttt tagatgagct tcttttaatt
    22441 ccataatgat atcattttca tcaaaatcgt gaatgtttat tgttttgcca acctttaaag
    22501 tttgaacaaa tctatctacc tttggatcat atgaaagtcc tataaaggga acttttctaa
    22561 cagaagccat gattaaacca tgtaatctca ttgaaaggat ataatccatt gtattagtta
    22621 aagataaaat attgactggt gaattatccc cttcataaac cagggatgaa ggaatttctt
    22681 tactcatttc cctggcaatt tcaatatcat ccggatgatg cataggaaca aatacaattt
    22741 cccatccttc atttaatgcc cattttaaaa ccttttttac ttttttataa aaagaagaat
    22801 ccaccttcca atccctaagg tataccccta gtcttttctt attatcttga ttaggtaaca
    22861 aaactcttcc tttttcttct tctgaactaa gtgtcatagc catgacagga tccggaacaa
    22921 cttctatttc tttttttata cctaatcttt ctaactcctc tttagattct tcgtccctga
    22981 cagaaatata atccgcttta ttaacaatcc atttaataag aatgcggtta aatttcatat
    23041 tcactggtcc tatcccctgg gaataaagaa cgagtttttt acggcatatt tttgcaataa
    23101 gcatgactga aagataatag cctatgcttt tccaactcgt tacatcctgc agcaggctgc
    23161 ctcctccact gattaataaa tcagcttttt ttattgcctg cagcacttgc ctcatactcc
    23221 agcgatcaac ggccttaacc tgatatgttt ttgccgtttc ttccggagaa ttagacaaaa
    23281 ctgtaatatc gatatctttt attttctctc tcaaactgtg gatgatagca tataatactg
    23341 cttcatcgcc tgcattatta aaaccgtaat atcccgataa aactacactt cccataggcc
    23401 ccatcttctt cctaatcctt ccagcccttt gagcacaaaa attaaaatta ttcctccaag
    23461 aaatccaatc caaattccat ggaacgttct aatcagtgaa atcattatag gcgtatgaat
    23521 atgcgcatag gtattcacaa tagatatttg acctatcaca gctcccaaaa ggaccgggaa
    23581 atatttctct ttatagccgt agtataatga aacaagcatt aaaggatgtc caattaaaaa
    23641 ttctttcgtt ctgggacgaa ctccaagcat tttatttaat aaatttctaa tcattgcttc
    23701 gtagcttgac tttggtcctt cattacctgt tcttaaaaca taaataataa gagcaaggcc
    23761 taatatacca aggacaaaaa gcgctaagta ggtaacagga ctaaaagata aatccttaat
    23821 ttttttcatt tttaaattcc ctcttttgta ctcaatgagt aatggaacta aaagaagagg
    23881 gaaaatatgt gccactttag cacctctaaa taaatctatg gtcagcacaa aagagctttc
    23941 tgataaaagg cctataatcg ttaatgcccc accaaaggag aataaacaaa ctttaataaa
    24001 agcaataaat aactccttcc atgtcttttt cttttcagag atacagctaa tcactgccca
    24061 tgtaggataa aggatagccg cagctaaagc catgagctgt cttgctaaaa tgggatggac
    24121 aaataacagt cctgtccatg ccataaaagc agcagcagca agcagccatg cccatttttc
    24181 cttatctatc cacctgccaa ataaaacaag caaaaagatt ggtcccaatc caatcagcca
    24241 aagaaatacc ggattggctg aaggcaaatg aacaggctct ggatctgaac taatcttata
    24301 acctttattg gatagagcct gatgaatacg actaacgatt ttataccaat cttcataatc
    24361 tttatcaggc tgatccgtag caggcatttt tacaagcaaa gcctttaaat tcctttctgt
    24421 agctgccagt aaatacctgt caattacacg ttcaggcgta agagttgatg tttccccatc
    24481 tgctaaagta tgtagtctta tcgaattata tctgccttct gttacgcccg cacgaataag
    24541 ggaatataag cccttttgct tttctgaaaa gaattccact ataccaattt tatgattttt
    24601 ggcaaaatct ttcattgtgt ctgtaccatg accaggaact gtgacatcat taaaataaac
    24661 cgttcccaga ttcggaatat tatctacgtc atttaataca aattttaaag catcttctgt
    24721 gacattgggc caagctctta cttgaggaga tatcagaaaa ccttcattag caataatttc
    24781 taaatcatct gttggatagc ctgtcccaat cgtaaacaga ttattgctgg aagctcctgc
    24841 atcaataaat aactggttat ttatttctat tattggagca gaacctaact tatattcaat
    24901 atgtttttga atactgtcac gaattctctc atcagggacc cacagataat agtggctggg
    24961 atttatatct gaaaaatttg tatcggctat tttttcaaaa agcattaact ctgttcctga
    25021 aaatacagta acctctcctt gttcttccca actgaccata ttacctctta catcactttt
    25081 tatagtttgc tcttttatta atacgcctcc aacacctgct tcttttaatc tgacgaggac
    25141 ttcttcaaga ggtttaccat atatttctgt cgctttttta atatcgttat aagttactag
    25201 gatttgaaca tccttttgac tttgttcagc gctaaagcgt ttaaccccct gaataccgga
    25261 cagaattaaa gatattataa tgatagaaac aggaagtatg tttataaacc ttttcataga
    25321 gtgcctccta gattgttctc atctttttaa tccaatggta gtatatcata ttttcctctt
    25381 accataaata cagcatcctg caagacatag acacctctgc cgtcatctct tgggacaata
    25441 atcagatgat atttaggaat ctcttgtccg ctggaaatgt ctgttccttc agtcatatcc
    25501 tgaagtcctc ccagttccgc agtaatggcc agaccttttc cacctcttaa aatgatttca
    25561 gtcccctgat gtccgattaa actctttccg gcaggaactt cgaccacttc ataggtcatt
    25621 gaatagctcc ctgcctgaga gttattattc gaattatttg aagagagttt tccaattaaa
    25681 tccataaccg catctatttt ggagtctaca tagctttttg aaaccaaggg gtcctcatta
    25741 ctaccaggag aaactccaaa aactgttaca gctccaaaaa taaccccaag tacaataacc
    25801 aacaaaaaca ttaaaacaga acgattatgc ttttttttca tatgggcttc ctcctattat
    25861 caatccacta attataaatg ggcttaaatt cataagcaat ttccgaatct gttaatctaa
    25921 atctcaaaaa agacaaatca gatccgctta cacagatata acgtacccct tcaagaattt
    25981 gtacctgatt gctttgaccg gaacttatca caaaaatgtt tttgtttgtc tttgctttta
    26041 actctttcaa cagtttatgg aataattcag cttctgcagc atctgaaaaa gcttcattct
    26101 gaggattttt atccatgatg ataaataaat tatccttatc tgtatctaaa tcattttgaa
    26161 gccatttcca ttgttcagga ttagctgccc ggatacctcc attggaagca ttaatttgaa
    26221 taatccttgc attctttata tcattaactt gataattgtt attaaatttc atataaggaa
    26281 ccttaatatc catctcattg acagctgttc caaaataaat gctaaaagaa gcattctcat
    26341 tcatttgttt aactagttct ttttttatct cttcatcaag actattgctt atcagtccag
    26401 agactgtaat atcaaagcta ttttttgcag aaaccctttc taaagatttt tcatgtttat
    26461 cctttaaccg agtgctattt atcacattgg cttcatcaat ttctggcaaa gaaatacatg
    26521 aaagatggtc aatataaatt gtattttccc gttctgctgc gctgtttaag gtgaccgtat
    26581 atactttact taaagaaatg ggataggtta ccgaagaagg aatcgtcgcc tgaacatatt
    26641 tccagccagt ccagttaatt tccttagcaa aatcaataag gaattcctgg ccattggcat
    26701 cttttaaact tcctcttaac caatccattg agttatctcc ataaacccac attccaatcg
    26761 ctttagcatt attaagaata acaataggtt ctttaaaatt cacataggcg gcttgtgtga
    26821 cattcggatt gtttttaaag gaatatttca tcttgataga tttcgttcct tcatatttta
    26881 tatccgtgtc gatatcaaca actcctgtaa cggttgaagg atatcctgac cattctacaa
    26941 gtctgtcgcc ttcaaaagat tctaaaggcg ttaccttttc accaatagca acaggaacag
    27001 caatctttac atctccataa aaaccttcta tgataccctg accagttgta ttggctgcaa
    27061 taaactttcc attaataaca gttcctaatg aggggtcaac tttccatgtc attttagtta
    27121 agtcaatatt tcctttataa ccttcttcat ctaaaccaac aacccataaa ttaacgcctt
    27181 cccctggttt aagccggatt gtttcaggat gagctttcaa agcctttggc attcccatac
    27241 aagttaatgc aacctgtcct tttacattac ccacttgtgc tgtaattatt ccttctccag
    27301 cagacttggg gacaaagaca ttatttgaaa aattgccttg tatataatct acgtcccatt
    27361 ttatcgcact attcgcaata ggaacaggat ttaagttttt atcatagccg ataacttcca
    27421 aactaacagg catgttttga aatactctat gattattggg aatgattttt atccctgcca
    27481 gttcacccgc aggtgcatca aagaaaactc caagtccatt gactactttt ctttctgaac
    27541 catctgaagg cttatttaaa agacttagtt catcttttcc catgggcttt gctgctatcg
    27601 tcgtggaacc tcccccatcc agatgaatcg catcataaat atcatattcc ataagaagcg
    27661 atgtcatttc actatgggtt gcaccaacac tttgacctct gccatccaca gcaattaaat
    27721 acaaagtcct tccgtccctt gaaataccca aggctgttct gggacctctt acattaggag
    27781 atactaaatg acctggattt acaggaacag ttcctttatc tactattttt cctgctccac
    27841 ctaaagccat ttgaatactg gtcaaatcca cagaagtttg aagatcaaaa aatacttttt
    27901 gtcctacttt aaattcagcc cttttttgaa ccgctgttgg ctcatcaata acaataacat
    27961 atccgttttc aggaatttca acagtctctc cgcgctttga aacatatgta atcacaccat
    28021 cttgtacaac aaatttatat agagcaggat gacgactgtc aatttccgca gttgatgtat
    28081 aagaatttct gtcaagatat actgcatatt taaagctggt gattttgtta ataccagcta
    28141 ccccgatata aacaccattt tcattataaa acagcatttt tatcttacag tagtctataa
    28201 aaggatttcc ttcattatca atgaaaaagg ttgtccattt attcttgtca atattggtat
    28261 tgttatatgc agagattaat ttcccatccc tggcaaccat acctaaagaa gctgtaggat
    28321 tttttgacat atcaaaaaaa tcaccattta tccctgcaac ggcaccatta gactttatta
    28381 agtccgttgt tgtttctctc ttgttatatt ggttagtaga ttctaaaacg tctattctta
    28441 catcgggatt tgttaaatcc actttaagta tataggtgtc taaccagcca actgccgtta
    28501 agcgtctgtc tttaatatat gaaatacctt ttctgatttc ttcttcatgg acttctttat
    28561 acaagacatt cgttattgta gttgcttcag taataaatgg gaaaaaaact gttgtaaata
    28621 taaacagggc aacgattaca gaggctattc tacgctttct ttgcttcatt caattcaccc
    28681 ctaaatattg ttattaatag aaaactgtat taaaaataat acagtttcca aaccatacgt
    28741 tatttttcac ttaatcccag tataattcgt atatctatat cctggtcggt tatggattca
    28801 tccacaatga ttttgctgtt ttcaaagtat tgttttaaat cttctcctaa accttcttta
    28861 ttgacaataa tccttgttgc atcttctctg gtccctccat agtttcctat agaaactaca
    28921 ttatagcctt cttcttttaa tttatcccct gttctttgtg ccaaaccaga aacctgacca
    28981 ccatttaata cttctatgcg ggcatcctta ctactgattg tactttcact tttttcttct
    29041 cctttatcgt cactaaagaa tatctcatcg actaacttcc ttgttccttc ttcatcatga
    29101 atgaaataag atatacctcc aatggtcctt gcttctccag gaatcgtcgt catattgata
    29161 ttatctaatt ttatttcatc taaatattta acataagaaa gtgcatcatt aatgctaata
    29221 tctgtttcta catatttaaa caaattttta ataatcccag gtgcattcac cacattttta
    29281 ggactaagtg ctttttttgc aaaggcctgt aaaaataact gctgggtatt aattctgcct
    29341 aagtcaccat atcctcctcc cttgttgtct tttctccagc gaaccaactg ttctgcctga
    29401 gcaccattta aagtttgggg ccccgctttt aagttaatat acagccctcc tgcacggtca
    29461 acataataaa aatccctggg caaatcgatt tcaacaccac ccatttcatc aacgatgttt
    29521 ctaaagcctt ctaaattcat ctttacataa taatcaattt taataccaag tagtctttca
    29581 acttctttta cagaatactc atttgccttt tcttttccag cataagcatg aacttcatta
    29641 attttacatg tatctgggac ataagaattt ctgcttcgca tatcctgaag catagcatcc
    29701 gttaaaaata ccctggtatc tcttggaaca gaaatcagat caatcttttt ggtttcacta
    29761 ttaaaggtcg caacaataat gacgtctgtt cttatttcat cttcgtcaac gccaaaaata
    29821 gctacattgg tcctgacttt tttagatttt ttattaaaga agtccataat tcccttattc
    29881 tcttcaccgt tttcagcggt attgttttct gctaactcgt ctttattaaa atgtaaatac
    29941 cctgccgtac caatactggc aagaaccaga aaagaaaaaa caaataaaaa aacaatagaa
    30001 aagaattttt taactaaata gtacttccct tttttttgat tcttcttcga catattcttt
    30061 cactccatat tcctcaactt taatactttt ttaaatttaa tccgcattat tttcttactg
    30121 atctttctgg ttaaataatt cctccaatat ttctcttgtc ttgtccttat cctgcatttc
    30181 ataccataaa ccattgattt tttcaccata ggttggtatg gttaccatgt ttccttcctg
    30241 aaaaatcgta gaaacattaa gccctgttat gtattctata tatttcatac aatctaacaa
    30301 agaaaagtcc gtttcaatat attgatatcc ttccttgact aaagaaaata tctcaatagg
    30361 atttcttatc tccaatactt ttttacataa agctttcaag acctcctgct gcattttaat
    30421 tcttcctaaa tcaccgtcaa catatccact tcgatatcta accagttgtt cggctttttc
    30481 accatccaag gtctgtaagc ctgattctaa ggaaatatgt aaaccttgag actgatcatg
    30541 ataaaccatc cttttgggga cttctacttc gatacctccc actaagtcta caattgcctt
    30601 aaaaccgtct aaatctacct taacataata atcaatcgga atgcctacta ttttttcagc
    30661 aatttcctga ataactttga ctgcatcctc tttatctgta acattttgat aaacctctgt
    30721 taacttgcaa tagctaacat taacctgata acttcctctg atagaggaaa atttataatc
    30781 agaaaaatca atatataaat ccctgggtat ggatacacac tttaaagaat tttctttagg
    30841 attaaattgc accaccataa caacatctga taaattttta gacttgtcta agccaataat
    30901 cacggcagta atttttttac ttcgccttca tcttcctgtt tttcttcctt actattgtct
    30961 gtggctgcaa ttagattgtt tgaactatcc ccaccaggga aattgatttc gtaatctgac
    31021 tttgttgccc atttaacaat catattggat attataagcg ttgaaacaat tatcatgaac
    31081 attaagccca tgattaatat gattttaata tggtttctat tttcaaaaat atttcccata
    31141 ttattacacc tcttaatacc attctttata ttatcatata tttattacaa acatgcaaca
    31201 aatctataat attttattat caaaattcct taatttttat taaaaatttc gcgttcaata
    31261 aaaagcctga aggaaagtga ttgataatgt aaatgaatct ggctataata atctaaaatt
    31321 ataaaatgaa accttatcat ggtaacatta tttttgtttc ttagaaggtg ataatatgga
    31381 ttttaaaaat aaagctgtac tggtaaccgg cgcttcaaga ggcattggta aagaaatagc
    31441 tcttagtttt gcaaaaaagg gtgcaaacct gattatcaat agttccagaa gtaaagaaca
    31501 attagaagct gtaaaacgag aaatagaatc actgggctgc caatgttact cctttttagg
    31561 agacgtatca gactatcagt ttgttgcatc gatgtttgaa aatatttaca aacattttga
    31621 acaagtagat gttcttatca acaatgcagg tatctctcat atcggcttat tcacagaaac
    31681 cacagtcacc cagtggaaaa gaattattga tgttaattta aattccgtct ataattgcag
    31741 ttattttgcc ctgcctccta tgattaaaaa tcacagagga aatattataa atatttcttc
    31801 tatttgggga gtcactgggg catcctgtga agtcgcttat tctgcgtcca agggcggagt
    31861 aaatagtttt actaaggccc tggcaaaaga attaggcccc tcaaatataa gggtgaatgc
    31921 catcgcctgc ggcgttatcg atacggatat gaacacctgg cttagtcctg aagaaaaaag
    31981 tgaacttctt gaccaaattc ctttaatgaa aatgggaagc tcacaagatg tcgccaactt
    32041 atgtatttat cttgcctctg atgagtccaa atacataact ggacaaatca tccgtttaga
    32101 cggaggcatg atataaatat ttaaggattt gctttttaac ttttgtcagt acttcatggt
    32161 ccggattcct gtctcttctt ggaatatcca atgatgcctg atataaaatt ttagcaggtt
    32221 tttgcgataa aataattatt ttatctgcta aaaacaaagc ttcttccaag tcatgtgtta
    32281 tggctataat tgtattatta acttctttat ggagtttata tacttcttta tatagttttt
    32341 cttttaattt aacatcaatg gcctgaaaag gctcatccat caagagaata tcatggggat
    32401 ataaaaaagc cctgatcatg gaaacacgct gtttcatgcc cccgcttaat tctttgggat
    32461 aagaatgttc atattcttta agttccatta cctcaaggta ttgatctatt ttttttattt
    32521 cttttgcttt acagaccaat tctaaatttc ccctgactgt tttccatggc agtaatctgg
    32581 cttcctgaaa aataaaactt atctttttgc cttttaaatc aatatttccc ttatcataac
    32641 tttcaagatt ggcaattatt cttaaaagtg tcgtttttcc acaacctgaa ggacccatta
    32701 tacaataaat cttaccttcc tctatgtcaa gagaaaaatc atcaaaaaca actatttctc
    32761 caaagttttt tgatagattt ttaatcataa aatcaccgcc attttttcgt tactgaaagt
    32821 tcaatatact taaataatct gctaaatcca tgccccagga aaatcaacac tgctgtccag
    32881 acaaaaacct gtccaatttc aagattaatt cttgcaacac ccatagctgc gccaatccca
    32941 cttcttgctg tcagcaattc tgccatagca acccctttcc aggtcaggcc gcttgcaata
    33001 gaacaccctg ccataaaata tggaaaaatc tgcgggatat ataaataaaa aatcttcttt
    33061 cttaaagaaa agttaaatac ctgtgccatt tccattaaag atgaatccac ttcttttgtc
    33121 cctttcataa cattaaccat aataaaagga atgacaataa taaaaataat aaaaacagct
    33181 gctgccccat cacctgttcc aaaccataac cagcctaaaa tcagccaaga gacggttggg
    33241 gtcgtttgag taagcaccaa taagggatga aaaaaggctt cgcattcttc cttaagtccc
    33301 atcaaaaaac ccaaaggtgt cccaataata actgccagaa aaaaacccaa taaacctctt
    33361 tcaaaagaaa caaaaatatc cagccaaaat tcttttttag aaaggaaatt caaaaaaatc
    33421 tttgccgttt ccacaggaga aggcacaatt aaaggactaa gaaaaaaaga agcaatttgc
    33481 cagatcaata aaagtataaa aatagaaata agaagataaa tcttcttatt tcttataata
    33541 aaatccttca tcaggcagtt ttcctcctat catttctgct gaaagttcat ttaaagcctt
    33601 aaggtaagct tcaacatctg tttttgcatc ctctgctgtt gcaaattcta atttggtcct
    33661 tgtcatagag ttttcaaaaa ctgctgctgg tatttctaat ttttcttcaa ttaacccaga
    33721 tacagcttta gggtcactga ctacagtttc taaagcagtt ttataggcat tttgaaaatc
    33781 ttccacgata tccggatatt ctttagcaaa agaagtcctt acaacaatac cggtttgggg
    33841 caatctgctg cctttaccgt taatcttttc ccattcctgg ctataatcca tggatatttc
    33901 catattttca gccttcaaca aacattgggt taccaaaggc tctggcaata ctacaatatc
    33961 tgccttccct gcaatggtcc attgaagcat ctccggagaa gttccatagg atatggttat
    34021 ctcctcttcg ccaatgccat tttggcttaa taaatatctt gtaataacat caggactggc
    34081 accttgagcc cctacaaaga gctctttacc tcttaaatct tcccaggatt ttacctcgcc
    34141 gtttcttgat aataagtaaa gaatacccca tgtgtttaca ttgagcaatg aaacatccgc
    34201 ttccttattg tacaattttg aagccacatt aaccggaaga accgtaaaat ctgcttctcc
    34261 cctggcaact cttgtggttg cttcttctac attctgatag agaataaatt ccacattatc
    34321 ccccagcatt ttgtcttcta ccatcttaac caggggtgac gttggcggtg ccacgggtgc
    34381 ctggattatt attttatcag ggatgcttgt tttttgatcc tttcccttac tgcatcctgc
    34441 tgctaatatg acaataataa atacaaggaa aatggcttgt atttttttca tattatctcc
    34501 tcctcttcta atagtattcc aacttaaaac tataacatta tagtacatat ctttttagtt
    34561 gtcaaaaata attattaaat gataaagtta cgtaataaat aataaccaat agatattttg
    34621 aatacaagaa tataccccaa atgcaaataa aagcattcca aaaaaaagta gtccccattg
    34681 aattttccct ttttcttctg tagcagatgc ctttgcctct tcttttttat gtatggtcat
    34741 gagattaata ttcccgtttt cacttaaatt aaaactcact tttgttccga cagaaaaatt
    34801 aaacttaaaa tctataatgg aatccggcat atttacggaa taaaataaag cttcagctct
    34861 ttgaccgtct ttttcatatt ctatttttat cctttgtcct tgttcatttt cttcaatggc
    34921 tgtgattata ccatcataaa cgccggattt attattaatc attttctcta caggattagg
    34981 ttccattacc gtattttttc ctaaagccat aaccataaaa aaaagaccga aaataattga
    35041 aaaagcagac atggtaatgc tcattttttc acctcaaaag ctttatccta taagtttatc
    35101 ttacaataat tttaaacttt ttccaatcat tccttaaaga actttttctt ttcagacaaa
    35161 aaattatttt cattatatcc attagttaat gagatttgac taaaataaaa agctcaattt
    35221 atttaaatga attgagcttt tctataatat ttattaagta ttttattatt aaatcctctg
    35281 cctttttatt caataaataa tttcaaaacc tctattatcc tatatttgac aatgcttatt
    35341 ctctgcaatc aggtaatcta ctactatctt tataccttaa caatcgctaa atccttataa
    35401 actttataag catttttaat gtctgtaaat atgtttttct ttattatgta cgtgccaata
    35461 caggaaatct taaatgattt ttttcaaata aatcaataat aatatctcct tttacaaaaa
    35521 gtgtcgtatc ttcttcataa atttccttat attcataatc gtcttttaat aaaggtgtaa
    35581 aaccaaaaac tactttttta atttcttttc caaaagttga aataattcca tccatatcaa
    35641 ccttttccgg ttgttcagtt acaaataagt ttgctcacgg agtgacaaag tttgctcgtg
    35701 atcaaatgta gttgtttcaa ggcttctcgc tgtttcttcc cttgtagtga caaagttt
//
LOCUS       DAOSUD010000013        35643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_833_length_35643_cov_22.684360, whole genome shotgun sequence.
ACCESSION   DAOSUD010000013 DAOSUD010000000
VERSION     DAOSUD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 35643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35643
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_833_length_35643_cov_22.684360"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1791
                     /locus_tag="PLG49_03160"
     CDS             100..1791
                     /locus_tag="PLG49_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOQ16347.1"
                     /translation="MKKTDKRKLGDLLVQADMLTVKQLKKALAEQERTGEKLGEILVK
                     KGWVTEQNIVEVLEFQLGIPHVDLSKYAIDPKAASMISESLAKRYMLIPIQIENDRLV
                     VAMSDPLNVYAIDDVKLATKMEVMPVIASLADVKQAIDRMYGVSKTVSIAEEFRRQMQ
                     EKPQKKNGLDEEMDEADVLNSPAVKLVNSFIEQAIRRNASDIHIEPFENYVRVRLRID
                     GQLHEILKTDISALNAIVTRIKILGKMNIAEKRIPQDGRIGVTLDNNQLDLRVNTLPT
                     IYGEKVVIRLLYRTGMQLRKEELGFCESDLKKFESLLMKPHGIILLTGPTGSGKSTTL
                     ATALRELNKPNVNIITVEDPVENMIEGINQVNVNAKAGLTFASALRAILRQDPDIVMI
                     GEMRDTETASIAVRASITGHLVLSTLHTNDAPSSITRLIDMGIEPYLVATAVEGIIAQ
                     RLVRRICKNCKTAVHPTSDEAEILKIPKNSLIYKGKGCQACNNTGYKGRLAVHEVLVL
                     DSSMREMIVKGANSDEIREFAINHGMITLWDNTKRNVLEGNTTVEELLRVAYGQE"
     gene            1802..2863
                     /locus_tag="PLG49_03165"
     CDS             1802..2863
                     /locus_tag="PLG49_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOQ16348.1"
                     /translation="MDIDKLLAYAVENKASDTHITVGFPPTLRIDGKLEPLDLPKLTA
                     EDTEKMVMHLLRFRGKKDVLLRDGEVDFSYGIPSVGRFRVNVFKQRGTFAAAIRTVTV
                     GIPTLDELKLPPVLKSLAQNVRGLILITGPTGSGKSTTLAAMIDLINSQRSCHILTLE
                     DPIEYLHKHKKSIVNQREIGPDSKSFASALRAALREDPDVILVGEMRDLETISIAITA
                     AETGHLVLSTLHTVGAAETIDRIIDVFPPHQQEQIRVQLANVLKGVVSQQLLKLQTGR
                     GRIVATEVMVTTPAISNLIREGKSHQIHSMIQTGSKYGMHSMDMNLAELYKRGLIDRQ
                     QAINYAQDQEAIRKLLEID"
     gene            3002..4213
                     /locus_tag="PLG49_03170"
     CDS             3002..4213
                     /locus_tag="PLG49_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ16349.1"
                     /translation="MPVYVYQARNTRGELISGEVTFETEEEIRSYLSARKLIPVEIKT
                     KSALDTDLSKLPIFKSKVKTRDIALFCRQFAIMLESGITIGATLDIVRRQIENPTLKE
                     IVQKVYEDVQKGRSLSEAMGDHPEFPPLLVSMIESGEVSGSLDEVMVQMAGHYEKNLD
                     VQRKVKSALTYPAITVTLILILMVVLIAFVIPNFLSMYASENLELPWVTRALMSITEF
                     TRDNFLIIMTTIISLIAGFFAVRRTPKGKHFFDELVLKLPIASNVNKMVITANFCRTL
                     STLLKAGTPIIKSMEIVRRVVGNEVAMDVITNSIEEIKQGTGLAISLHESDLFPDMLT
                     SMIRIGEETGALDFTMEKTADFYEAEADLAIQNATTVINPAITIVVGAVVGFVVLAVM
                     LPAFNMLEAML"
     gene            4406..4795
                     /locus_tag="PLG49_03175"
     CDS             4406..4795
                     /locus_tag="PLG49_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ16350.1"
                     /translation="MFKKNLKNEKGFTLIELIIVIAIIAIISAILAPSFARMTARSRL
                     KADISSARELTREAALYNADKGTYPSAVTDLVNDKYIDSAPTPQTRGATFVWDGTNNR
                     FVVDVHGDQELTQLIGTLSADEKSFAK"
     gene            4879..5652
                     /locus_tag="PLG49_03180"
     CDS             4879..5652
                     /locus_tag="PLG49_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOQ16351.1"
                     /translation="MYLFYILVFITGLVIGSFLNVCIYRIPLKESIVFPASHCVNCKH
                     QLAVLDLIPVFSFIFLKGKCRYCGTRISIQYPVIELVNALLFLCIFIHFGFSYQSFFF
                     ALFSSLLLVISVIDFNEGIIPNPLIIFGLILGLIYIVFTTIGTQSWDIIRDGLTGSLL
                     GAFIIILIILISRGGMGAGDYKLMAVIGLFLGYKKIILVFFLAVIIGGIFAAFLLMFK
                     SKERKSAIPFGPFISTAALIALFYGDLIIEWYLSSFVFF"
     gene            5687..6238
                     /locus_tag="PLG49_03185"
     CDS             5687..6238
                     /locus_tag="PLG49_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ16352.1"
                     /translation="MKKLFNHFGFTIIELIIIMAIMSILAAIVVPSFGVVQRNRINSV
                     SSQIAMDINRVHTYARTHYNPIVGGDYEYKIVFSDPVTVNTKDYFTKYELCGPEVYEG
                     TQPEIPAGVLIRPVMGHSTEILIKEIQFDEKGRVRIKDAAGSCYDWNAINSLIRISVE
                     IEDFDVKKEVLVNQLTAKTTITE"
     gene            6254..6841
                     /locus_tag="PLG49_03190"
     CDS             6254..6841
                     /locus_tag="PLG49_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ16353.1"
                     /translation="MRNIIQRINDEKAFTLIEVIIALTIFAILSVSIMGIMINFSSTT
                     GRTQRQIETLENARIVLDFMIEEIRRAKGIQIDNLTGNQILALEMNDTPGIDTSASVP
                     DVVFEFDSLAKQVYYNGVILSDNIELIEFEPANLVDADGNLDPDLDKNKDGLIDDNFK
                     IRIRALIPQDNHVKKDVDYDITGEVSIKYKKILAN"
     gene            6866..7933
                     /gene="pilM"
                     /locus_tag="PLG49_03195"
     CDS             6866..7933
                     /gene="pilM"
                     /locus_tag="PLG49_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOQ16354.1"
                     /translation="MGQLLGIEIGNSNIKFVLGTKKGRIIHVQNYGIDPTPNNAVRDG
                     FIVDMEAVYNAAFHLLKKKHITEQNAAITIQSTSIITRDVVVPYMEEKELRNVLEYQK
                     EEYFPIDVSDYQTDFKILEVLNTDEGKKLNVLLVAAPNNMINGAMDLLRRLNLKVKFI
                     DIAANSVANLYAHKSMSFQGEEPYSIMLLDIGGQTTTATIVSDGKILFTRTILYGFDE
                     LNNIIAKEFSEESYEQVEIFKRRYGAIYDDDKAPDDDLYGSHISHIIKPIIDTNLVQE
                     ISRVLDFYYTRNNARKVKTIYLIGGGAFLKNFDRYIGKAFGINTVRGDILDNIKARDA
                     VNFKNDFLYLSNVLGLITRVQ"
     gene            7946..8548
                     /locus_tag="PLG49_03200"
     CDS             7946..8548
                     /locus_tag="PLG49_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16355.1"
                     /translation="MKDINLIPQEYILIEKRKKFGFFMIIVGIFAIAVLAVLITTPMR
                     IINEKKQTLAQLNAELKDPKYNIIDELKKELSQKQDEKVLLNNLLEQVDYNTIASRTT
                     LDILTGHLPPGMHISEIQVCNEKIQGDYPYIRIFGAANDFRTVSDYAGQLMNLPFIRD
                     TVINTQTETIADKGYYAKQIEYDITLYVDKTKGSVSQDEE"
     gene            8538..9854
                     /locus_tag="PLG49_03205"
     CDS             8538..9854
                     /locus_tag="PLG49_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16356.1"
                     /translation="MKSKTNWGLVILIGLFIIGSIVLYWTYILHPQLQQIKQLNNDIE
                     DVKRQIQQKETELLQLDSVRAEIAECENEIATANRKILPYKEFAHKMYFFESSAQLND
                     LIIKRYTVRNGKRKSVSLSGEYIEDIDKLPNGEFQGDLEYRELLVEMEVGGYYESVNT
                     YLEQLKANIKPFTIYYVKLTTDIDETFKEYMRGYYNIDLNSNMEGYVLATIYLRTFSL
                     YNGQQADDEIINRNYMEYDFEYNNPIREIEKLQEKIYKEIGGNTAGPRTDELRDFIKG
                     VSNLVKPPITPVNPGTEEPGVKTFKIAIKDAYESGDNYYIVGPGDSGEYTTVQATTIQ
                     PATFDLVLNPNGYQYSLTTVDEGTKSFSKQMELGECRLVINSTVLELRESQLLHTTIN
                     ITNNTGKVLRVEVNGSYKDRISIFSTKSSTQINPGEVVDNIMVVYK"
     gene            9875..11005
                     /locus_tag="PLG49_03210"
     CDS             9875..11005
                     /locus_tag="PLG49_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ16357.1"
                     /translation="MVRVKDWLKNYIIKNESGVTLVEIIVAIAIFSIFIIPIFSMVLT
                     SGNNTANSKRYYDATVMSENLSEEIKKKITEKLAKYDPLHDTTINDPIGQKGVPVEID
                     SFLEMTESAFNNIFKANDYYYEVYIKEIDATTDSYSMGDPTDLYILKKGLMTVPVVEG
                     DSFPATPLNTVDFTLLSENYVTYFKSEYTYDSTYNYNPTGAPSKIVEDLTFGYINGTW
                     NTAIPKNGTDITIKYDGTKRKYDIKVGKNPTLNTTDVIRLNIDMTRANDDLKNEEEML
                     IRVENESKAKVIISTFRSTDQYDNKIKILPIQKVVNDGDQSFEEIMSNSGNIYVEMNT
                     KFIPRKNYLIKIIVRDARQSQNGKILKEMIDIYAHDYRILTY"
     gene            11028..13685
                     /locus_tag="PLG49_03215"
     CDS             11028..13685
                     /locus_tag="PLG49_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16358.1"
                     /translation="MISYLKALLKSEKGSALVMVVFLTLVMLLIGGAIMAISFSNVEV
                     SNAYKKTSNLYHVAEAASEKLLDNVNKVIIAEMPRIMKEASDEAKSTVLKKVEASDFG
                     GLKYDDSPDGSETNAYKYNYYLYDSVTDKMNLYEEALTKKTFALINDKFINEPELLKI
                     EYEVKDGRNKIVVTGELERVPDPVAAAAGVGVDDPEYYKKDKLLLNVHASLQNGTDIL
                     SELDLEGDVILAGLNEHEEILLEQYEWKKKTDPSGSRESIIPNAFRSPVLTFGDFVVA
                     NGAEVVVKGDLRARGYIPSSTIDHPYPELEEYGGVYVSNGSRLKVEGDVVTLANLHTI
                     APTNSTATTVIDVTGNVIAHSVAVEDDYPGYCDPTSRTWSDFIKDHHILIRGDVYVDN
                     DVAIDRYAEESTITIMGNLFGINNRPGVNNDPNQASGVYCIGNNSLITIGKNAFVHGQ
                     AWISFDNGARFSRLYESIGEPYEEVDYLPEYLNGNPIGNSIYLAQRKDYIRKDKIALN
                     TSAYFYAPARISANGYFYVTKSNPADVDTSIGETQINTHGAFDEQEEVLRLFNAGGIS
                     ESNLMQINPNWSAGIQGAEDLTAVDIISSGGKAFAYLSGDESMNLLKRKYFSDGGSGF
                     YSMGAFRGIEGYMLVRRDIFIKNINTSAVSVNLMDFDKDIVYAETPSGATTEVYNAFN
                     DFTTEEVTWSTDNPVFFVRTAENEEKIINISDFHVEDKEDEPLETIIVDRGNGTIILR
                     ADGSLQKLKALIVSNGRVVFDGVSEFEGIIITKGKHEGYSLTTRQLVSGSHAGILVRN
                     NLEITYDPDILFKVKCKNFGVKRVVMDYLGLTNYNAKPDDSEKIFMNATIKTEESQKI
                     QLSPESVFYTSDIEAFKGVRYELETLRMK"
     gene            13703..15094
                     /locus_tag="PLG49_03220"
     CDS             13703..15094
                     /locus_tag="PLG49_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16359.1"
                     /translation="MKYIHWKSWLAFVLMSLLLVGCSFLNSTSRDVTDLISEEVENIE
                     EKEEVDVKPDQDKIKNELVKFFNQYYNAPTNVLIDLNRYPIIPNDNYREVYESFKQAS
                     LERLENYMTAELEKNLKKNYFTTDIHFPRFLLINGNVVVNYNKVEDLEYNLISQEDIN
                     DGKDKAYTYLIHVKVKANTIPQERFYELFSFNPETNYYDLINPNIIIEDSEKDEILLE
                     CTYVAKIQDKNNKIKFYYLKEEGEKAVSEEDRRTIVNNSFAKPINYIDKPFSADSLLI
                     REFVNKFFNMNKDDHLYYEYAFDANFDIFAQALTDLAIAEYLVPDENNYRNQYPKMII
                     PTKDDIINLNVNIQDIKVDVHSRTSRGGRVYIVNIPAVAELSDYSKEKVEYRYYFQVE
                     EVEIDEETVTRITRIQYEYIKRPFPAFDLEISGSDISADNTQTDQNANENEGATTNNS
                     EGTKGSENVPSNF"
     gene            15136..15642
                     /locus_tag="PLG49_03225"
     CDS             15136..15642
                     /locus_tag="PLG49_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOQ16360.1"
                     /translation="MLRQLFPFDYIDSVFELNIEELMRLKIKGLIFDIDNTLVPYDVA
                     YPNQKLISFFEKLKEKGFKICLVSNNTEERVIKFNEDLKLYAIHKASKPRRKSFLKAM
                     NLMGTHKDNTVVIGDQVFTDVWGGNRLGLKTILVVPVSARDEWITKIKRGLERIIIRL
                     YQRQRSKK"
     gene            15642..16517
                     /gene="aroE"
                     /locus_tag="PLG49_03230"
     CDS             15642..16517
                     /gene="aroE"
                     /locus_tag="PLG49_03230"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOQ16361.1"
                     /translation="MTVVNFVQGDTRVYGLIGNPIAHTLSPLIHNSLNKILGNNAVYV
                     PFEVFSQGLKAALEGAYELNIQGLNVTYPYKEAVIPHLIEMDAYGKQIGAVNTLKKSE
                     GGYVGYNTDACGLLMSLKEEQIQIENHNILIIGAGGAAKAAAMALSSQKAKKIYIANR
                     TEKRAEDLAKAVQQYYSLPIEVLPLSDIHLLNDVDICLQTTSLGMHPQEGQSPVNDDS
                     FFNKITVAVDIIYNPWESLFLKQAKKAGCKTVNGFGMLFYQAVKAYEIWIGQEISKDK
                     IASLYQSLKKKLKEK"
     gene            16539..17000
                     /locus_tag="PLG49_03235"
     CDS             16539..17000
                     /locus_tag="PLG49_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ16362.1"
                     /translation="MKRKDGFTLIELIIVLSIILIIAAIVVPSLHITERYALKAAAME
                     LKSDILYTKTKAVKDSKRYWMKIYKDSNLYFITSDAFGPHHKVVYLNEGIEIEEAVFS
                     TDNTIKFTIKGTTGSGGRICLRSKHFRVKITVVPATGRVKIYSIEKIKTNH"
     gene            17037..17309
                     /locus_tag="PLG49_03240"
     CDS             17037..17309
                     /locus_tag="PLG49_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOQ16363.1"
                     /translation="MLEIKNYMEDLVFEEMDAVIKDIQCCNCEKCRMDIAAIALNSLQ
                     PHYVVTEKGRLYTKINTLQQQFGIDVLTALIKAAIIVKRNPQHSDE"
     gene            17302..17829
                     /locus_tag="PLG49_03245"
     CDS             17302..17829
                     /locus_tag="PLG49_03245"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOQ16364.1"
                     /translation="MNNHLNIVLIGFMGCGKTTVGKNLSKKLSYPFVDSDLEIEKEIG
                     MTISDYFKKYGENNFRLIEEKIIQKLASGTRKIIATGGGIVKNPGNIYHLKQNGLIIY
                     LKASAEHIYNNLKNDFSRPLLNVEDKYTRIQSLLEERKSLYEKYSDYIVEVDGKAVDE
                     ITKSIIDYLEEGYRL"
     gene            17826..18314
                     /gene="aroQ"
                     /locus_tag="PLG49_03250"
     CDS             17826..18314
                     /gene="aroQ"
                     /locus_tag="PLG49_03250"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOQ16365.1"
                     /translation="MKKILVIHGPNLNFLGIREKTIYGETSFEALNNLLLKKGMSMGF
                     KVEVFQSNSEGAIIDCLQKAYYDKVDGIIINPGALTHYSYALRDAIASIDIPTVEVHI
                     SNIYKRETFRHQSVTAPSCIGQISGFGINSYILGLHALYEILNYHETSKENSDEGKTD
                     KD"
     gene            18289..19356
                     /locus_tag="PLG49_03255"
     CDS             18289..19356
                     /locus_tag="PLG49_03255"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOQ16366.1"
                     /translation="MKERLTKIRKLLIDNQLDGIMIHSPVNRKYLSGFTGSAGILLIS
                     QSLAVLLTDFRYIEQAKKQATDFIIIDYMAEGLYNEINKFIKKENINRLAIEAEKLSY
                     KEYREYNEKLDAQLVPTEKLVENIRKIKDKDEIKRIKQAAAIGDEAFRHILSFLKAGI
                     SERDIALELEYYMKKHGASDLSFSTIVASGAFSALPHWEPGERKLEVGDFVVMDFGCI
                     YKGYCSDMTRTVVIGKASSRQKEIYDIVFQAQMAALEGLGPFKRGKEIDKIARDYIEM
                     KGYGSCFGHGLGHCVGMEIHENPRLSKNEEEEFLPGMVVTVEPGIYIPDFGGVRIEDL
                     VLITDDGIEILTKSPKELIEI"
     gene            19439..19996
                     /gene="efp"
                     /locus_tag="PLG49_03260"
     CDS             19439..19996
                     /gene="efp"
                     /locus_tag="PLG49_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOQ16367.1"
                     /translation="MVSAGDFRNGLTIELEGEIYTVVEFQHVKPGKGAAFVRAKLKNL
                     KTGGIIEKTFRPTEKLPKAHIERKDMQYLYNDGDLYYFMDSETYDQIALNKDQLGDSL
                     KFVKENDMVKILSHNGLVFGVEPPLFVELEITHTEPGFKGDTATGATKPAVVETGATI
                     QVPLFVEIGDKVKIDTRTGEYLSRV"
     gene            20116..21084
                     /gene="spoIIIAA"
                     /locus_tag="PLG49_03265"
     CDS             20116..21084
                     /gene="spoIIIAA"
                     /locus_tag="PLG49_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOQ16368.1"
                     /translation="MKTKEVILSFLSSRIKSLFYHIPEEEFEKIYEIRLRVNKPLILL
                     KEGQYYFVRSDGYLTKKLEQAFIVFQKDIIDTAQLMSDYSMYALEQELKNGYITLQGG
                     HRVGITGKVVIEKGVIKTIKYFGAMNIRISHQIIDCARELFPHIINGENIYHTLIISP
                     PGCGKTTMLRDLIRLVSDGVENLFSGITVGVVDERSEIGGCYQGIPQNNVGIKTDVLD
                     GCPKAEGMRMLLRSMSPRVIAVDEIGKMEDIFAIEDVMNAGVKLICTVHGSSLADIQR
                     KPVLSELINKKIFERFVVLSNRKGPGTVEDIIDGINLNSLMKGREL"
     gene            21090..21599
                     /locus_tag="PLG49_03270"
     CDS             21090..21599
                     /locus_tag="PLG49_03270"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HOQ16369.1"
                     /translation="MTGALMIITGTAFIGYLFDRFELYRMKDLENIKRALLFLKGEID
                     YMIASLPVAMEEVGSRVGTNIGKIFSHVGESMKNKRGYSAEKLWKESVELYKDSTYLS
                     KEDINNLLALGTALGYLDKEMQKNNIDLLLVYLEDEINRLKLHRHKNGRLYKTLGFLG
                     GILIVILLY"
     gene            21626..21820
                     /gene="spoIIIAC"
                     /locus_tag="PLG49_03275"
     CDS             21626..21820
                     /gene="spoIIIAC"
                     /locus_tag="PLG49_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOQ16370.1"
                     /translation="MDITIVFKIAAVGILVSVLNQVLVRSGREEQAMLTTLAGLIVVL
                     FWIIQYISDLFKTVQTLFQL"
     gene            21832..22218
                     /gene="spoIIIAD"
                     /locus_tag="PLG49_03280"
     CDS             21832..22218
                     /gene="spoIIIAD"
                     /locus_tag="PLG49_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HOQ16371.1"
                     /translation="MEIGQIVIIGLISVALALMLEKHNPEWSMYIRIVAGILIFFIII
                     QKLGILLEIAKKMSEKIDMDFVYIGIVFKVLGISYISEFGAQLCKDAGESSIASKIEF
                     AGKVLIITVSSPVMLALIDIISDIIP"
     gene            22232..23389
                     /gene="spoIIIAE"
                     /locus_tag="PLG49_03285"
     CDS             22232..23389
                     /gene="spoIIIAE"
                     /locus_tag="PLG49_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOQ16372.1"
                     /translation="MKKVKILLIGLLWLFMPVKAYSQSVDEIWTRQIESLDFSRIDQM
                     VTNIKSQKENPFLQGFDFKDIVIKSIKGELDFSLGNIIKSIIFLFFKEMPAYFSLIGK
                     LMVISMLSAFLNSLAESFQSKSTSNVAFYVCYMVLIILLLQSFQIAIQLSQSTLENMV
                     LIIQASLPALLTLMMASGELTSGAVYEPVLLFAIQIMGVLIKKILLPFIFFTAILSVI
                     NSLSDKNLLKKMLELFNQAIDWLIKGIAILFIALMSLSSFTAPVLDGIINRAAKTAVG
                     VVPVVGETLTGAVDIVMNCSLLIKNAVGVGVIILLAVYCILPLIKILLFFMVYKLTAA
                     LIEPISDKRIVNCISDTGEACIKLFSLVFTSSLIFIVAITILIGIGSMTLN"
     gene            23411..24040
                     /gene="spoIIIAF"
                     /locus_tag="PLG49_03290"
     CDS             23411..24040
                     /gene="spoIIIAF"
                     /locus_tag="PLG49_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HOQ16373.1"
                     /translation="MIDYLGNWLRNIAFFIIFTVFLEMLMPDGSFRKYIRMLLSLILI
                     LILTRPVGSFLLEASSVADRTIINELEFERQSILKETIVFQEKQDDLIIETYKEKLEM
                     QIIKLIESNSHYSPQVIEIQINEDRSSQNFGAIEAIKILLTEKEDHNARDNTIGVVKP
                     IHINTSIKNENKTENSNINPEIEKNIKNLLINFYNLSSDNIHITVQKKY"
     gene            24051..24650
                     /locus_tag="PLG49_03295"
     CDS             24051..24650
                     /locus_tag="PLG49_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16374.1"
                     /translation="MGKIMEWLKLQDKKNKNLLYNITFFIGIGVLLIILGDTAFNNLP
                     RKLEEKRDNLVSNEGNLKEMNKTYEDVIEERLKEIFSKMEGVGKVEVMVTISYGKEIF
                     LAEDVNSNYSRTIEEDSQGGKREITSEDIQSKTVMQNTGSGSTQPVILKEKYPEIKGV
                     LILAEGGEDPRVKQQLTAAAETLLGVPAHKVQVFKMNKE"
     gene            24663..25391
                     /locus_tag="PLG49_03300"
     CDS             24663..25391
                     /locus_tag="PLG49_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOQ16375.1"
                     /translation="MFVLKRNQIIVTALVAMIAIAGYLNYTDSQQVKDADIVYNENLD
                     ITDTIPDDAMIMGEMDLTDEMEEGIIGEAISTDPEILAQINSADLGEDFDSIVETAAG
                     EESAVADEGEDDGAAVFVNSSTDTPYFAQAKLSREQARAKEREILSEILNNKSLDKEK
                     KSQAADEMLKLQRRIEKESAMEAMIEAKGFKEVYVRISDDRVDVVVNKEKLTEQELAQ
                     IESIVRSTTGYDVDKIQIIPLKSH"
     gene            25490..25903
                     /locus_tag="PLG49_03305"
     CDS             25490..25903
                     /locus_tag="PLG49_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOQ16376.1"
                     /translation="MDERNVSSMAESNQIGQIQIADEVIAIIAGLAATEVEGIAGMAG
                     SFTGGIAEILGKKNLSKGVKVEVGESEVSIDLFLVVKFGVKIPEVALAVQKKVKNAVE
                     TMTGLSVIEINLHITGVHFDKNKADNTNQEAEFFE"
     gene            25999..26421
                     /gene="nusB"
                     /locus_tag="PLG49_03310"
     CDS             25999..26421
                     /gene="nusB"
                     /locus_tag="PLG49_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOQ16377.1"
                     /translation="MSRRRAREHVFILLFQMTFINQEETDERIELYFEQHSHIKEEDK
                     KYITEELKAVLSKLETIDELISQHSKKWKIERMSRVDLSILRLAVFEMQYREDIPNSV
                     AINEAVELAKKYSGDQSPSFVNGVLGSIASSLGEPYDE"
     gene            26411..27637
                     /gene="xseA"
                     /locus_tag="PLG49_03315"
     CDS             26411..27637
                     /gene="xseA"
                     /locus_tag="PLG49_03315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ16378.1"
                     /translation="MMNKKVLTVSDVNHYIKALIDSDYVLSNIWVEGEISNFKRHSSG
                     HIYFTLKDKDGAISCIMFRSSAAFLRFDPQNGMNVIVKGSVSVYEKTGQYQIYVSQME
                     QKGIGTLYEAYERLKEKLEKEGLFLQSHKKAIPAFPEVVGIITSPTGAAIRDIIQISK
                     RRNPNIKLVVYPVLVQGEQAPGEIAHAIYEMNQWGQADVLIVGRGGGSLEDLWAFNEE
                     IVARAIFESKIPVISAVGHETDYTIADFVADLRAPTPSAAAELAVPLLKDLRLRVHIA
                     YKTLNHLMEDKIYSELEKLKKINQSVPFRKPLDRIYKKQQDLDSLEKILNNSMKHILE
                     KKRSQFGKEIKALEALSPLNTLLRGYSITLSKEGRNISSIGDVKVEDAIYIKLKDGII
                     DAVVKGIQEDEEGFDG"
     gene            27630..27881
                     /gene="xseB"
                     /locus_tag="PLG49_03320"
     CDS             27630..27881
                     /gene="xseB"
                     /locus_tag="PLG49_03320"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ16379.1"
                     /translation="MAKKKPLSFEEGIKRLEELVELLQEGDIRLEDALKYYKEGMELS
                     LLCSKQLEEAEKQVVELQKDFEGKFVEKVFDAWEKDNEL"
     gene            27871..28758
                     /locus_tag="PLG49_03325"
     CDS             27871..28758
                     /locus_tag="PLG49_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ16380.1"
                     /translation="MNYKEALEQKIKLINHYLDKLFNSIPDSQAKIIYDAMAYSIFAG
                     GKRLRPVLMLSAFEAVGGEGEIIYPFACAIEMIHTYSLIHDDLPAMDNDDLRRGKPTN
                     HKVYGEGMAILAGDALLNKAYELMIKASLTCSGREALEAMDIIATCAGTEGMIGGQVI
                     DLLSEGRQVDMETLLFIHKNKTAAMIEASLKAGGILGKGTKEEIEALGQLGYCIGMAF
                     QIQDDILDITGSQEKLGKAINSDIKNEKATFVSIVGLEASRKYVEKLTKQGISALEVF
                     GEKAAFLAWLSDYLMTRTY"
     gene            28771..29214
                     /locus_tag="PLG49_03330"
     CDS             28771..29214
                     /locus_tag="PLG49_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOQ16381.1"
                     /translation="MQLIQAIWNNNVLKAAVVAWFIAQLLKVIVVLIQSRKLDFSRLV
                     GSGGMPSSHSAFVMAMCVNVGETVGYDSPLFGVAVVIGFIVMYDAAGVRRAAGKQAAV
                     LNKIIEEIENMDFHFEERLKELLGHTPVEVIAGALLGIVIGLIFC"
     gene            29245..31110
                     /gene="dxs"
                     /locus_tag="PLG49_03335"
     CDS             29245..31110
                     /gene="dxs"
                     /locus_tag="PLG49_03335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOQ16382.1"
                     /translation="MDGIHSPKDLKKLNADKLNILAKEIRAFLIEKISHSGGHLASNL
                     GVVELTLALHYCFDSPKDKIIWDVGHQSYVHKILTGRKDQFDRLRKYKGISGFPKHKE
                     SIHDIFDTGHSSTSISVALGLAAARDLNNEKFSVIAVIGDGSLTGGMAFEALNNAGRS
                     NSDLIVILNDNQMSIAPNVGGLSKYLNKLRTEPVYLEVKEDISQALKKIPGIGESLEK
                     TVKKTKEGIRYLLVPGSLFEELGFTYVGPVDGHNIKELVTVIQRVKKMKGPILLHVNT
                     VKGKGYSFAEKYPSRYHGVDTFDIETGESLRKDNKESYSDVFGKTLVELAKENKKLVA
                     ITAAMPDGTGLKEFSKKFPQRFFDVGIAEQHAVTFAAGLAKGGFHPVFAVYSSFLQRA
                     YDQIIHDVCIQNLPVVFAIDRAGIVGPDGETHQGIFDLSFLSHMPNMTIMAPKDKSEF
                     VEMLKYSFSINGPVAIRYPKGEAPDLFPSVHQPIEYQRAEILYEGKDAAIIAVGKMME
                     KAKIIVENLKEKGYHLTLINPRFIKPVDEESIVELAKQHNKIFTMEDNVLSGGFGSKV
                     LELLAKHHIKDVEVRIFAFPDEFIAQGSVEELYREYGLDAETMEEEIIRQLTAKG"
     gene            31118..31930
                     /locus_tag="PLG49_03340"
     CDS             31118..31930
                     /locus_tag="PLG49_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOQ16383.1"
                     /translation="MSSKERLDLLLVKKGFFDSREKAKRSIMAGLVFVDGIREDKAGA
                     KVSTEARLEVKGTMKYVSRGGYKLEKALDCFAIHLEDKIALDIGASTGGFTDCMLQKG
                     AKKVYAIDVGYGQFAWELRNDERVVCMEKTNIRYVREEDLDDKGDFASIDVSFISLCK
                     VLRPVMNLLKEEGEIVALIKPQFEAGREKVGKKGVVRDPKVHEEVIMNVIEYGLSENL
                     YLKGLTHSPIKGPEGNIEYLVYFVKNSNNHIYHADHQLIREVVRKAHETLNG"
     gene            31965..32828
                     /locus_tag="PLG49_03345"
     CDS             31965..32828
                     /locus_tag="PLG49_03345"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOQ16384.1"
                     /translation="MKKAGLIVNIKKDINLDSSIKIMDWLMQRNCLVYIPENLAKILE
                     KPHLSKKEEEIYQDSDFIVVLGGDGTFLSAARKAAIYDTPILGINLGTLGFLAEVEKA
                     SSLQALEKVLAGNYSIEKRMMLEVKVKDCKKNNQQKLICLNDIGVTRSSLSRIINLKV
                     YINNQFVDYYSADGIIVSTPTGSTAYNLSAGGPILDPNSKMMVITPICPHSLSARSIV
                     VSDEDYIEIEICENHNCDIILTIDGQLGYQLDNHDIIQVTKSTYMTKIIKTSDYNFYD
                     ILRKKIVGIRK"
     gene            32833..33285
                     /gene="argR"
                     /locus_tag="PLG49_03350"
     CDS             32833..33285
                     /gene="argR"
                     /locus_tag="PLG49_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOQ16385.1"
                     /translation="MKLKRQAKILELINNYDIETQEELADRLREAGFEVTQATVSRDI
                     RELKLTKVANEKGIQKYSVIGQSEKNISSKYIRVFKEGFVSMDIAQSILVVKTITGMA
                     MAVAAAMDAFNYPEIVGTIAGDDTIFCAVRSDQEALKLMEKLNRILNS"
     gene            33301..35001
                     /gene="recN"
                     /locus_tag="PLG49_03355"
     CDS             33301..35001
                     /gene="recN"
                     /locus_tag="PLG49_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOQ16386.1"
                     /translation="MLVHIYIKNIALINEITIDFKEFLNILTGETGAGKSILIGSINF
                     ALGERGSRDIIRTGEENALVELLFFVENDEILELIKNQGIEVEEDRYVLITRAINQSG
                     RTVCRINGQTVTTGMLRQVSSKLIDVHGQHEHQSLLDVGKHIELLDSFCKDELAALKK
                     ELESKYKEYKNIEKQISSLIGDEKEIERKIDLLQYQLDEITNAKFEIGEEEQLQNQLK
                     KLSNSEKLVRGMEELYGLLYGNDYENHSTSLFLSEALRLLRDLADVDDELMPLYENLE
                     NIQLLLDDMVRDIRQYKDQIHHDPYELKAVEERLDLLFDLKRKYGNSIEEILLYKDKI
                     EEELERIVNSEETLKNLKNQYALLEDEIKRLSGKISDIRKEKAKIIEERIESILAELA
                     MKEAKFSVMFSQKDSFNENGWDNVEFLISTNPGEELKPLAKIASGGEMSRIMLALKCV
                     LADIDEIGTLIFDEIDTGISGATAQKVAEKLGYISRKRQVICITHLPQIAAMGDCNYK
                     IEKRIDENKAISSIKALKEDEVIYELARLISGAQITDISLKNAEEMRLLAKHFKAKLN
                     "
     gene            35121..>35643
                     /locus_tag="PLG49_03360"
     CDS             35121..>35643
                     /locus_tag="PLG49_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOQ16387.1"
                     /translation="MNNKKFLRFAVLFVIFVLLISPYAASYYYIPKEIKMMAGRNHDF
                     EFNLPLQAKISSEKIGVLKFNNKPIESNNFQIDLRSPFSVQPTEQATLDVKLKFLGIV
                     PLKTVKVDIISQKELVPCGMTVGVNVSTDGIMVLGTGTVISEDGKEYTPSRGILKTGD
                     LILKVNEKSIENKE"
BASE COUNT        12464 a         4866 c         7372 g        10941 t
ORIGIN      
        1 ggtagattaa aaatatttta ataatttatt tacatttatt tagaaaattg ttataataaa
       61 agtaagaata agaatatggt gagatgaaga ggagtaaaaa tgaaaaaaac tgataagaga
      121 aaactaggtg acttattagt tcaggctgac atgctcaccg ttaaacaact taagaaggca
      181 ttggcagagc aggaacggac gggcgaaaaa ttaggtgaga tattagttaa aaaaggctgg
      241 gtcacagaac aaaatatcgt tgaggtttta gaatttcaat tagggattcc tcatgtggat
      301 ttatccaaat atgctattga tcccaaagct gcctcaatga taagcgaatc tttagccaaa
      361 agatacatgt tgattcctat tcaaatagaa aatgatcgct tggtcgttgc aatgagcgac
      421 ccattaaatg tttatgcaat agatgatgta aaattggcga caaaaatgga ggttatgcct
      481 gtaattgcca gtcttgctga tgtaaagcag gccattgaca gaatgtacgg ggtaagcaag
      541 acggtaagta ttgctgagga attccgcaga cagatgcagg aaaaacctca gaaaaagaat
      601 ggtttggatg aagaaatgga tgaggcggat gttttgaatt cgccggctgt taaattggta
      661 aacagcttta tagagcaggc tatcagaaga aatgccagtg atattcatat tgagcctttt
      721 gaaaattatg taagagttag attgagaata gatggtcagc ttcatgaaat attaaaaacg
      781 gatatttcag ctcttaatgc gattgttacc agaataaaaa tcttaggtaa aatgaatata
      841 gcggaaaaaa gaatacctca ggacggacgt attggggtaa cgcttgataa taatcagtta
      901 gaccttcgtg taaatacttt gcctaccata tatggagaaa aagtggttat ccgtttgctt
      961 tacagaacgg gtatgcagct taggaaggaa gaactgggct tttgtgaaag tgatttgaaa
     1021 aaatttgaga gtctcctcat gaaacctcat ggaatcatct tgctgactgg tcctacaggt
     1081 agtggtaagt ctaccaccct ggcgacggca ttaagagaat taaataagcc caatgtaaac
     1141 attataacgg ttgaagaccc agtagaaaat atgattgaag gtattaatca ggtgaatgta
     1201 aatgcaaaag caggacttac ttttgccagt gccctgaggg caattttaag acaggaccct
     1261 gatattgtta tgattgggga aatgagagat acagagactg ccagtattgc tgtaagggca
     1321 tctattacag ggcatttggt tttaagtacc cttcatacta atgatgcacc cagttctata
     1381 actcgtttaa ttgacatggg aatagaacct tatttggtag caacggctgt tgaagggatt
     1441 attgcgcaaa gattggtaag gaggatatgt aaaaattgca aaacggctgt tcatccaacc
     1501 agtgatgagg ctgagatttt aaaaattccg aaaaattctc ttatttataa aggtaaaggc
     1561 tgccaggctt gtaataatac aggttacaag ggcagattgg ctgttcatga agttttagtt
     1621 ttggattcta gcatgcggga aatgattgta aaaggtgcaa attccgatga aataagagaa
     1681 tttgctataa atcatggtat gattacgctt tgggacaata ccaaacgaaa tgttcttgaa
     1741 ggtaatacca ccgtagaaga attactaaga gttgcttatg gtcaagagta ggaggatata
     1801 gatggatatt gataaattgc ttgcgtatgc tgtagaaaat aaggcttctg atactcatat
     1861 tactgttgga tttcccccaa cccttcgtat tgacgggaaa ttggaaccat tggatttacc
     1921 caaattgacg gctgaagaca ctgaaaaaat ggtcatgcat ttattgagat tcagagggaa
     1981 aaaagatgtt ttactgaggg acggggaagt ggatttttct tatggaattc cttcagtagg
     2041 tcgtttcagg gtcaatgttt ttaagcaaag aggtaccttt gcagctgcta tccgtacagt
     2101 aaccgtaggg atacctaccc ttgatgagtt aaaattacct cctgtattaa agagtttagc
     2161 tcaaaatgta agaggactca ttttgatcac tggccctaca ggaagtggaa aatcaaccac
     2221 attggcagca atgattgatt tgattaacag ccagagaagc tgtcatattc ttacattgga
     2281 agatcctata gaatatcttc acaaacataa aaaaagtata gttaatcaaa gagaaatagg
     2341 gcctgacagt aaatcctttg cctcggcctt aagagctgcc cttcgtgaag acccggatgt
     2401 tattctggtt ggggaaatgc gtgatttaga aacgatttca attgcaatta ctgcagcaga
     2461 aacaggacat ttggttttat caactttaca tacggtaggt gctgctgaga cgatagacag
     2521 aattattgac gtatttccac ctcatcaaca ggaacagatt cgtgttcagt tggctaatgt
     2581 attaaaaggg gttgtttcac aacaactttt aaaattacaa acaggtagag gaagaattgt
     2641 agcaactgaa gttatggtga caacacctgc aatttcgaac ctaatccgtg aaggaaaaag
     2701 tcatcagatt cattcaatga ttcagacagg tagtaagtat ggtatgcatt ctatggatat
     2761 gaatttagcg gaattatata agagaggctt aattgataga caacaagcta ttaattatgc
     2821 acaagaccaa gaagccatca gaaagctgct tgaaattgat taaatgatta taatggtatt
     2881 attcaattta tcatatatta taatccttgt ttaataatag aaaatatctg agatgattac
     2941 ttcgttgtat gacttaatga cacaaacaat gcacggatag gactgctaaa ggaggggtat
     3001 gatgcctgtt tatgtatatc aagctagaaa tactagaggc gaattaatca gcggggaagt
     3061 aacttttgaa acggaagagg agatacggtc ttatttatcg gcaagaaagt taattcccgt
     3121 agaaattaaa acaaaaagtg cactggatac tgatcttagc aaactgccga tttttaaatc
     3181 aaaagtaaag acaagagata ttgctttatt ttgccgtcag tttgctatta tgctagaatc
     3241 tggtattaca attggtgcaa cccttgatat tgttagaaga cagattgaaa accctacatt
     3301 aaaagaaatt gtacaaaagg tttatgaaga cgtccaaaaa ggtagaagct tatcagaagc
     3361 catgggtgac catccggaat ttcccccttt gcttgtaagt atgatagagt ctggggaagt
     3421 ttctggtagt ttagatgaag taatggttca gatggcaggc cattatgaaa agaatctgga
     3481 tgttcaaaga aaagtaaaat cagcactgac ttatcctgcc ataacggtta cgttaattct
     3541 cattcttatg gttgtactta ttgcttttgt tattccaaac ttcttaagta tgtatgcctc
     3601 tgaaaacttg gaattaccat gggtaacgag agcactcatg tctataactg aatttaccag
     3661 agataatttt ctcatcatta tgaccactat cattagttta atagcaggct tttttgcagt
     3721 aagaaggact ccaaagggaa aacacttttt tgatgaatta gttttaaagc tacctattgc
     3781 cagcaatgtc aataaaatgg ttataacggc taatttttgc agaacattaa gtacactgtt
     3841 aaaagcagga acaccaatta ttaagagtat ggaaatagta agaagagttg ttggtaatga
     3901 agtggcaatg gacgttataa caaattctat tgaagaaatt aagcaaggta caggactagc
     3961 catatccctt cacgagtcgg atcttttccc ggatatgctg accagcatga tccgaatagg
     4021 tgaagagaca ggtgcgcttg actttacaat ggaaaaaaca gcggactttt atgaagcaga
     4081 agcagatttg gctatacaaa atgcgacgac ggtcattaat ccggccatta ccattgttgt
     4141 tggtgcagtt gtaggtttcg tggtacttgc agttatgctt cctgctttca atatgctgga
     4201 agctatgcta taaagacaga agctgaatga aaaatctgtt ttttgtctaa ttttagctaa
     4261 atatatattt tacaatatat atttaaattc acttaagggg gtgagggaca aaaaagtaag
     4321 ggggtaaaat atagagatat ataaaacaat aatttctagt catcatccaa aatttattta
     4381 aaatttatga aggggagatg tgtaaatgtt taagaaaaac ttaaaaaatg aaaaaggttt
     4441 tacacttatt gaattaatta tcgttattgc aatcattgct atcatcagtg ctatccttgc
     4501 tccaagtttt gcaagaatga ctgcaagaag cagacttaaa gctgatatta gctctgcaag
     4561 agaacttacc agagaagcag ctttatataa tgctgacaaa ggaacttatc caagtgctgt
     4621 tacagacttg gtaaatgaca aatatattga ttcagctcct actccacaaa ctagaggtgc
     4681 aacatttgtt tgggatggta caaacaaccg tttcgttgtt gacgtacatg gagaccaaga
     4741 acttacacag cttattggta ctttatccgc tgatgaaaaa agctttgcta aataataata
     4801 aatataattc tttcttatca ggcacattat gtgcctgata agaatttata ctaaatggag
     4861 gaacagaaga ccgtgcagat gtatttattt tatattttag tctttatcac aggtttagtc
     4921 attggcagtt ttcttaatgt ttgtatttat agaatccctt taaaagaatc cattgttttt
     4981 ccggcttctc attgtgtaaa ttgtaagcat caattagctg tcttagactt aatcccggta
     5041 tttagtttta tttttttaaa gggcaaatgc agatactgtg gaactagaat tagtattcaa
     5101 tatcctgtca ttgaattagt aaatgctttg ctttttttat gtatctttat tcattttggt
     5161 ttttcttatc agtcattttt tttcgcatta ttttcttcat tactcttagt gatatctgtg
     5221 attgatttta atgaaggaat tatacccaat cctcttatta tttttggact tattttaggt
     5281 ttaatatata ttgtttttac aacaatagga acacaatcat gggatattat cagagacgga
     5341 ctaacaggtt ccttgttagg agcatttatt atcattttga ttattcttat ttcccggggc
     5401 gggatggggg ccggtgatta taaactaatg gcagtcattg gcttattttt aggttataaa
     5461 aaaattatcc ttgtattttt ccttgccgtg attattggag gcatatttgc agctttttta
     5521 ttaatgttta aatccaaaga aagaaagtca gctatacctt ttggaccatt tatatctact
     5581 gcagctctta ttgcgttgtt ttacggagat cttattatag aatggtattt atccagtttt
     5641 gtatttttct aattataaat atagagtcaa gtgaggaatg gtggtaatga aaaaattatt
     5701 taaccatttt ggctttacga ttatagaact gattattatt atggccataa tgtctatttt
     5761 ggctgccatt gttgttcctt cttttggtgt tgttcagagg aaccgtatta atagcgtttc
     5821 ttctcaaatt gccatggata ttaaccgagt tcatacttat gcccgaacac attataatcc
     5881 tatagtgggt ggggattatg aatacaaaat tgtttttagt gatcctgtaa cggttaatac
     5941 aaaagactat ttcacgaaat atgaactttg tggtccggaa gtatatgaag gaactcagcc
     6001 agaaattcct gctggagtct taataagacc tgtgatgggg cattctacag aaatactgat
     6061 taaagaaatt cagtttgacg aaaagggtag agtaagaata aaggatgcag caggtagttg
     6121 ttacgattgg aatgcgatta attcactaat caggattagt gttgaaatag aagattttga
     6181 tgtcaaaaag gaagttctag ttaatcaatt aactgctaaa acaactatta cagagtagtt
     6241 ggtaggtgat atgatgagaa atattatcca aagaattaat gatgaaaaag cttttacttt
     6301 aatagaggtt atcattgctc tgacgatttt tgctattctt agtgtgagta taatgggaat
     6361 tatgattaac tttagttcta cgacaggaag gacgcaacgg caaattgaaa ccctggaaaa
     6421 tgccagaata gtgcttgatt ttatgattga agagataaga agggcgaaag gaatacaaat
     6481 cgataatttg actggtaatc aaattctggc acttgaaatg aatgatacac cggggattga
     6541 cacatctgcc agtgttcctg atgttgtttt tgaatttgac tctttagcca aacaagttta
     6601 ttataatgga gttattttat cagataatat agaattgatt gagtttgaac ctgctaatct
     6661 ggtagacgca gatggtaatc ttgaccctga cttagataaa aataaggatg gcttaatcga
     6721 tgacaacttt aaaattagaa taagggcact gattccccag gataatcacg ttaaaaaaga
     6781 tgttgattat gacattacgg gggaagtttc tattaaatat aaaaaaattt tggctaatta
     6841 aattaatcaa atgaggggtg tgaatatggg acaattgctt ggcattgaaa ttgggaacag
     6901 taatattaag tttgtactgg gcacaaaaaa agggaggatt attcatgtcc aaaactatgg
     6961 aatagatcca acacctaata atgctgtccg tgacggcttc atagtagata tggaagctgt
     7021 ttataatgct gcttttcatt tgttaaaaaa gaaacatatt acggagcaga atgctgctat
     7081 taccatacag agtacatcca ttattaccag ggatgttgtt gtaccatata tggaagaaaa
     7141 agaactaaga aatgtccttg aataccaaaa agaggagtat tttcctatag atgtgtcaga
     7201 ctatcagacg gattttaaga ttttggaggt cttgaataca gacgaaggca aaaagttaaa
     7261 cgttttatta gttgctgctc ccaataatat gattaatgga gctatggatt tactaaggcg
     7321 gttaaattta aaggttaagt ttatcgacat agccgctaat tcagttgcta acctttatgc
     7381 ccataaatcg atgtccttcc agggagaaga accttattcc attatgcttt tagatattgg
     7441 tggacaaacc acaacagcca ctattgtgtc tgatggaaaa atattattta cacgtaccat
     7501 cttatatggt tttgatgagt taaacaatat tattgctaaa gaatttagcg aagaaagtta
     7561 tgaacaagta gaaattttta aaaggagata tggggctatt tatgatgatg ataaagcgcc
     7621 tgatgatgat ctatatggaa gccatatcag tcatatcata aagccaataa ttgatacaaa
     7681 tttagtgcag gaaattagcc gggtattaga cttctactat actagaaata atgcaagaaa
     7741 agtaaaaact atttatttaa ttggtggagg cgcttttctt aaaaattttg acagatatat
     7801 tggcaaggct tttggtataa atactgtcag aggagacata ttagacaaca ttaaagcaag
     7861 agatgcggtt aatttcaaaa atgattttct ttatttgtca aatgttcttg gtttaattac
     7921 gagagtacaa taggggggaa tcaacttgaa agatattaat ctaatacctc aggagtatat
     7981 acttatagaa aaacgaaaga aatttggttt ttttatgatt atagttggca tttttgcaat
     8041 cgcagtttta gctgttttaa ttacaacgcc tatgcgaatt attaatgaaa aaaagcagac
     8101 cttagcgcaa ctcaatgctg aactgaaaga tcctaaatat aatattattg atgaattaaa
     8161 gaaagaactc agtcaaaaac aagatgaaaa agtcttatta aataatcttt tagaacaggt
     8221 agactataat actattgcaa gtcggactac tttagatatt ttgacaggcc atttgcctcc
     8281 aggaatgcat atttcagaaa tacaggtttg taatgaaaaa attcaggggg actatcctta
     8341 tatacgtatc tttggagcag caaatgactt caggactgta tctgactatg cgggacagtt
     8401 aatgaatttg ccatttatca gggatactgt aatcaatacg caaacagaaa cgattgctga
     8461 taaaggttat tatgccaagc agatagaata tgatatcaca ctctatgttg acaaaacgaa
     8521 gggatcggtg agtcaagatg aagagtaaaa ctaattgggg gcttgtgata ctgattggac
     8581 tttttattat cggatcaata gtcctttact ggacgtatat tttacatcct caacttcagc
     8641 aaatcaaaca attaaataat gatatagaag atgtaaagcg gcaaattcaa caaaaagaaa
     8701 cagaattact gcaattagat tcggtcagag ctgaaattgc tgaatgtgaa aatgaaattg
     8761 caactgccaa tagaaaaata cttccctata aagaatttgc tcataagatg tatttttttg
     8821 agagctctgc tcaattaaac gatttgataa ttaagagata tacagttaga aatgggaaaa
     8881 gaaaatcggt ttcactttca ggtgaataca ttgaagatat tgataagctt ccaaatgggg
     8941 aattccaggg agatttagaa taccgtgaat tgcttgtaga gatggaagta ggcggttatt
     9001 atgaatctgt taatacttat ttagaacaat taaaggctaa tattaaacct tttactattt
     9061 attatgtaaa actcactacg gatattgatg agactttcaa agagtatatg cgtggttact
     9121 ataatattga tcttaatagc aatatggaag gttatgttct ggctactatt tatttgcgaa
     9181 ccttttcctt atataatggg caacaggcag atgatgaaat cattaaccgc aattatatgg
     9241 aatatgattt tgaatataat aatccaatta gagaaattga aaagctccag gaaaagatat
     9301 ataaagaaat tggtggtaat acagcgggtc ctagaacaga tgaattaagg gattttatca
     9361 aaggagtcag caatttagta aagccaccca ttacaccagt gaatcccggg actgaggagc
     9421 ctggtgttaa aacctttaaa attgccatca aggatgctta tgaatcagga gataattatt
     9481 atattgtagg tcctggtgat tctggagaat atacaactgt gcaagcaaca acgattcagc
     9541 cagctacttt tgatcttgtt cttaatccta atggttatca atatagttta acgaccgttg
     9601 atgaaggaac aaaatctttc agcaagcaaa tggaactggg agaatgtagg ttagtcatta
     9661 attccacagt gttagaacta agggaaagtc agttattaca cactacaatt aacataacca
     9721 ataatacagg taaagttttg cgtgttgaag tgaatggtag ttataaagac agaataagta
     9781 ttttctcaac taagagttca actcagataa atccaggtga agttgtggat aatataatgg
     9841 ttgtttataa ataatgaggt taaggagaat cgctatggtt agggtaaagg attggttaaa
     9901 aaattatata atcaaaaatg aatctggtgt tactctggtt gaaattattg tggcaattgc
     9961 tatattttct atttttatca ttccgatttt ctcaatggtt ctgacctcag gaaataatac
    10021 ggctaacagc aaaagatact atgacgcaac cgtaatgtct gaaaatcttt cagaagaaat
    10081 caaaaagaaa attactgaaa aattagccaa gtatgaccca cttcatgaca ctaccataaa
    10141 tgatccgatt ggtcaaaagg gcgttccagt tgaaattgac agttttttag agatgacaga
    10201 atcagctttt aataatattt ttaaagcaaa cgattattat tacgaagttt atattaaaga
    10261 aatagacgcg actactgaca gttattctat gggggatccc acagatttat atattttgaa
    10321 aaaaggtctc atgaccgttc ctgtagttga aggagattct tttcctgcta ctcctttaaa
    10381 tacagtagat ttcaccttgt tatctgaaaa ttatgtgact tattttaagt cagaatatac
    10441 ttatgattca acttataatt ataatcctac tggtgctccc agtaaaattg ttgaagactt
    10501 aacttttggc tatataaacg gtacgtggaa cactgctatt cccaaaaatg gaacggatat
    10561 aacaatcaag tatgatggta caaaaaggaa gtatgatatt aaagtaggta aaaatcctac
    10621 tttaaacact acggatgtta ttagacttaa tattgatatg acaagagcca acgatgattt
    10681 aaaaaatgaa gaggaaatgc tgataagagt tgaaaatgag tctaaagcta aagtaattat
    10741 atccactttc cgttcaacgg atcaatatga taataaaata aaaattttac ccattcaaaa
    10801 agttgtaaac gatggtgatc aaagcttcga agaaattatg agtaacagcg gaaatattta
    10861 tgtagaaatg aatactaaat ttattccaag gaagaattat ttaatcaaaa ttattgtaag
    10921 agatgcccgt caaagtcaaa atggaaaaat actaaaggaa atgatcgata tatatgcgca
    10981 cgattatcgt attttaacat attaacgata aaggagggat atagggaatg attagttatt
    11041 taaaagcact ccttaaatct gaaaaaggta gtgccctggt tatggttgta ttcttgaccc
    11101 tcgtgatgct ccttattggt ggggccatta tggcaatatc tttttcaaat gttgaagtaa
    11161 gtaatgctta taaaaagact tctaatttgt atcatgtcgc agaagcagct agcgaaaagt
    11221 tactggataa tgtcaataag gttattatcg ctgaaatgcc cagaattatg aaggaggctt
    11281 cagatgaagc caaatcaact gttttaaaaa aagtagaagc atctgatttt ggtggattaa
    11341 aatacgatga ttcacctgat ggaagcgaaa caaatgctta taaatataat tattatttat
    11401 atgattcagt tactgacaag atgaatttat atgaagaggc attaaccaaa aaaacttttg
    11461 cactcataaa tgataaattt ataaatgaac cggaattatt aaaaatcgaa tatgaagtta
    11521 aagatggaag aaataaaatt gttgttacag gtgagttgga aagggtgcca gaccctgttg
    11581 cggcagcagc aggtgtaggt gtcgatgatc ctgaatatta taaaaaggat aaattattat
    11641 taaatgttca tgcgagctta caaaacggaa cggatatttt atcagaactt gatttagaag
    11701 gagatgtaat tttagctggt cttaacgaac atgaagaaat tttgttagaa cagtacgaat
    11761 ggaaaaagaa aacagatcct tcaggtagca gggaaagtat tattcccaat gcttttagaa
    11821 gtccggtatt aacttttgga gattttgtag tagctaatgg tgcagaagtt gtggttaaag
    11881 gtgatttaag ggcaagaggc tatattccat cttctaccat tgaccatccc tatcctgaat
    11941 tagaagaata tggaggtgtt tatgtttcta atggttcgag actaaaagta gaaggtgatg
    12001 tggtaacttt agccaatctt catacgatag cacctaccaa tagtacagct acaacagtca
    12061 ttgacgtaac tggaaatgtc attgcccatt cagtagctgt tgaagatgac tatccaggat
    12121 attgtgaccc gacaagtcgt acatggtcag attttattaa agatcatcat attctgattc
    12181 gcggagatgt ttatgttgac aatgatgttg cgattgatcg ttatgcagag gaatctacaa
    12241 taactataat gggtaattta tttggtatta ataacaggcc aggtgtcaat aatgatccta
    12301 atcaggcgag cggtgtttac tgtataggaa ataattcttt aattaccatt ggaaagaatg
    12361 cctttgtaca cggccaggca tggattagtt ttgataatgg tgcaagattt agccgtttgt
    12421 atgagagcat tggagaacca tatgaagaag ttgactatct tccagaatat cttaatggaa
    12481 atccaatagg aaattcaatt tatctggctc aaagaaaaga ttatatcaga aaggataaaa
    12541 ttgcgttaaa tactagtgca tatttttatg caccagcaag aataagtgcc aatggttatt
    12601 tttacgttac taaaagtaat cctgctgatg ttgacacaag tataggcgag acccaaatta
    12661 atacacatgg tgcttttgat gagcaagaag aagttttacg tttatttaat gccggtggta
    12721 tcagtgaaag taatttaatg caaattaatc ctaattggtc agcaggtatt cagggggcag
    12781 aagatttaac cgctgttgat attattagca gtggcggaaa agcattcgct tacttatctg
    12841 gagatgaaag catgaacttg ctcaaaagaa aatatttttc agatggcgga tctggatttt
    12901 atagcatggg tgctttcaga ggcatcgaag gttatatgct tgtaagaagg gacattttca
    12961 taaaaaatat taatacatcg gctgttagcg taaatctgat ggattttgat aaagatattg
    13021 tatatgcgga aactccatct ggtgccacaa cagaagttta taatgcgttt aatgacttta
    13081 ctactgaaga agttacatgg tctacagata atcctgtatt ctttgtgaga accgcagaaa
    13141 atgaagaaaa aataattaat attagtgatt ttcatgtaga agataaagag gatgagccct
    13201 tagaaaccat tattgtagac cggggcaatg gaacgattat tttaagagca gacggtagtt
    13261 tacagaagtt aaaagcttta attgtttcta acggacgggt tgtttttgat ggagtgtcag
    13321 aattcgaagg cattattatt actaaaggta aacatgaagg ttattcactt acaacaagac
    13381 aattagtgag tggcagtcat gcaggaattc ttgttagaaa caatttagaa ataacatatg
    13441 atcctgatat tctctttaag gtaaaatgta aaaattttgg agtaaagaga gtggtgatgg
    13501 attatctggg attaactaat tataatgcga aaccagatga ttcagaaaaa atctttatga
    13561 atgcaactat taagactgaa gaaagtcaaa aaattcaact tagtcctgaa tcagtatttt
    13621 acacaagtga tatagaagct ttcaaagggg ttcgctatga attagaaacc ttgagaatga
    13681 aataacgaat ggagtggtga ctttgaagta tatacattgg aaatcctggc ttgcgtttgt
    13741 tttgatgtct ctattattag ttgggtgcag tttccttaat tctacttcca gggatgttac
    13801 agacttaatt tctgaagagg tggagaatat tgaggagaaa gaagaagtag acgtaaagcc
    13861 agaccaggac aaaatcaaaa atgaacttgt aaagtttttt aatcagtatt acaatgctcc
    13921 aacgaatgtt ttaatagact taaaccgtta tcctataatt cctaatgaca attacaggga
    13981 agtatatgaa agctttaagc aggctagtct tgaacgttta gaaaattata tgactgcaga
    14041 actggaaaaa aatttaaaaa agaattattt caccacggat atccatttcc cacgattttt
    14101 attaatcaac ggtaatgtag ttgttaatta caacaaggta gaagacctgg aatataattt
    14161 aataagtcag gaagatatta atgatggtaa agataaagcg tatacttatt taattcatgt
    14221 aaaagtaaag gccaatacaa taccacagga aagattttat gaattattca gctttaatcc
    14281 tgagacaaat tattatgatt taattaatcc caatatcatt attgaagaca gtgaaaaaga
    14341 tgaaatcttg cttgaatgta cttatgttgc caaaatacag gataaaaata ataaaattaa
    14401 attttactat ttaaaggaag aaggagaaaa agctgtttca gaagaagaca gaagaactat
    14461 tgtaaacaac agctttgcaa aacctataaa ttatattgat aagccattta gtgcagattc
    14521 ccttttaatc agagagtttg ttaataaatt tttcaacatg aataaagatg accatttata
    14581 ttatgaatat gcttttgatg caaattttga tatttttgct caggctctga ctgatttagc
    14641 aattgcagaa taccttgttc cggatgaaaa caattataga aatcaatatc cgaagatgat
    14701 tattcctaca aaagatgata tcattaattt aaatgtaaac atacaggata ttaaagtgga
    14761 tgttcattca agaacttcca gaggtggaag agtctatatt gttaacattc cagctgtagc
    14821 agagttgtca gattactcaa aagaaaaggt agaatacaga tattatttcc aggtagaaga
    14881 ggtggaaatt gacgaagaaa cggttaccag aattacaaga attcagtatg agtatatcaa
    14941 gagacctttc cctgcatttg accttgaaat aagtggatcc gatatttcgg cggataatac
    15001 acaaacagat caaaatgcaa atgaaaatga gggagcaaca actaataatt ctgagggaac
    15061 taaaggttca gaaaatgtac catcaaattt ttaaaatgac tgctttctaa tctatgattt
    15121 acagcgaggt gtggcatgct aagacaatta tttccttttg attatattga ttcggttttt
    15181 gagctgaata tagaagaatt aatgagactt aagattaaag gattaatttt tgatattgat
    15241 aatacgctcg ttccttatga cgtagcgtat ccaaatcaaa aattaatctc tttttttgag
    15301 aaattaaagg aaaaagggtt taaaatttgt ttagtttcca ataatactga ggaaagggtt
    15361 ataaagttta acgaagactt aaaactctat gccattcata aggcttcaaa acccagaaga
    15421 aagagttttc ttaaggcaat gaatcttatg gggacacata aagacaacac ggtggttata
    15481 ggagatcagg tatttacgga tgtatgggga ggtaatcgtt taggtcttaa aaccatttta
    15541 gttgttcctg tcagtgcccg tgatgagtgg attaccaaaa taaaaagagg cctggaaaga
    15601 attattatcc gtctatatca gcgtcaaagg agtaagaaat aatgacagta gtaaatttcg
    15661 ttcagggaga tacaagagta tatggtttaa ttgggaatcc aattgcccat actttatccc
    15721 ccttaattca taacagtcta aataaaattt taggaaataa tgctgtttat gtaccttttg
    15781 aagttttttc ccagggatta aaagctgctt tagaaggagc ctatgaacta aatattcagg
    15841 gcttaaatgt tacttatccc tataaagaag ctgtcatacc ccatctgatt gaaatggatg
    15901 catatggaaa acaaattggg gcagtgaata ccctgaaaaa atctgaggga ggctatgtag
    15961 gttataatac ggatgcctgt gggctgttaa tgtcccttaa agaagaacaa atccagatag
    16021 aaaatcataa cattcttatt attggtgcag gtggggcagc aaaagctgcg gccatggctt
    16081 tgagttcaca aaaggcaaaa aaaatttata ttgcgaaccg tacagaaaaa agagcagaag
    16141 atttggctaa ggctgttcag caatattatt ctctgccaat tgaagttttg cccctgtccg
    16201 atatccattt actcaatgac gtggatatat gcctgcagac gacttcttta ggtatgcatc
    16261 cccaggaagg acaatcccct gtaaatgacg atagcttttt taataaaatt acagttgccg
    16321 tagacattat ttataaccct tgggaaagct tatttttaaa acaggctaaa aaagctggct
    16381 gtaaaacagt caatggattt ggtatgcttt tttatcaggc tgtaaaagct tatgaaattt
    16441 ggatcggaca agagatctca aaagataaaa tagcgagttt gtaccaaagc cttaagaaaa
    16501 aactgaagga aaaataagga ctggaaagaa ggattttctt gaaaagaaaa gacggcttta
    16561 ctttaattga gcttattatt gtccttagta ttattttaat tattgctgca attgtagtac
    16621 cttctttgca tattacagag cggtacgcat taaaagcagc agccatggaa ttaaaatctg
    16681 atattctcta cacaaagaca aaagctgtaa aagacagtaa acgctattgg atgaaaatat
    16741 ataaagatag taatttatat tttattactt ctgatgcttt tggtcctcat cataaagttg
    16801 tatacttaaa tgaaggcatc gaaatagaag aagctgtttt ttcaacggat aataccataa
    16861 aatttacgat taaaggtacg actggttcag ggggaagaat atgtttgcgt tcaaagcatt
    16921 ttagagttaa aattacagta gtaccggcaa caggcagggt taaaatttac tctatagaaa
    16981 aaataaaaac taatcattga tagaggcaag aagtcaaatt ttgaggaggg gatttaatgt
    17041 tagaaattaa aaactatatg gaagatttag ttttcgaaga aatggatgct gttattaaag
    17101 atatccagtg ctgtaattgt gaaaaatgca gaatggatat tgctgccatt gcactaaatt
    17161 ctttgcagcc ccattatgtt gttacagaaa aaggaagatt gtatacaaag attaatacgc
    17221 ttcagcagca atttggtatt gatgtattaa cggcattgat taaggcagca attatcgtaa
    17281 aaaggaatcc ccaacatagc gatgaataat catttaaaca ttgtattaat tggatttatg
    17341 ggctgcggga agacaacggt aggaaagaat ctttcaaaaa agctgtcata tccttttgta
    17401 gattctgact tggaaattga aaaagaaatt ggtatgacga tatcggatta ttttaagaaa
    17461 tatggagaaa ataattttag gcttatagaa gaaaaaataa tccaaaagct ggcttctggc
    17521 acaagaaaga ttattgccac aggaggcggt atagttaaaa atcccggaaa tatttatcat
    17581 ttaaaacaaa atggtctgat tatttactta aaggcttcgg ctgaacatat ctataataat
    17641 ctgaagaatg atttttcaag gcctttatta aatgtagaag ataaatatac caggattcaa
    17701 tccttattag aagaaagaaa aagtctttat gaaaaatact ctgattatat cgtcgaggtt
    17761 gacggcaaag ctgttgatga aattacaaaa tcgattattg attatttaga ggaaggttat
    17821 cgtttatgaa aaaaatatta gttattcatg gacctaattt aaatttttta ggtatcagag
    17881 aaaaaacaat ttatggagaa acttcttttg aggctttaaa caatttactt ttaaaaaagg
    17941 gtatgagtat gggttttaaa gtggaagtgt tccagtccaa ctcagaaggt gctattattg
    18001 attgcctgca aaaagcttat tatgataaag tggatggaat aattattaat ccaggcgcct
    18061 tgacccatta tagttatgcg cttagggatg ctatagcctc gatagatatt ccaacggttg
    18121 aagtacatat ttctaatatt tataaaagag aaacattccg gcatcagtct gtaactgccc
    18181 cttcctgcat tggtcaaatt tcaggatttg ggattaacag ttatatatta ggacttcatg
    18241 ccttatatga aatattaaat tatcacgaga catcaaagga gaattctgat gaaggaaaga
    18301 ctgacaaaga ttagaaaact attaatagat aatcagcttg atgggataat gattcacagt
    18361 ccggttaacc ggaaatacct tagtggtttt acgggctctg caggaatatt gttaatttct
    18421 cagagtttgg cggttttgct gacagatttc cgttacatag aacaggccaa aaagcaggca
    18481 actgatttta tcataataga ttacatggcg gaaggtttat ataatgaaat aaataaattt
    18541 atcaaaaagg aaaatataaa ccgtctagct attgaagcag aaaagctttc ttataaagaa
    18601 tacagagaat ataatgaaaa gctggatgcc cagcttgttc cgacagaaaa gcttgtagaa
    18661 aatatcagaa aaataaaaga taaagatgaa ataaagagaa taaagcaggc agcagcaatt
    18721 ggagacgagg ctttcaggca tattttatct ttcctgaaag caggtatatc tgaaagagat
    18781 attgctttag aattagaata ttatatgaaa aaacatggag cttcggattt gtcttttagt
    18841 accatagttg cttccggggc tttttctgct ttacctcatt gggaacctgg agaaagaaag
    18901 cttgaggtgg gggattttgt tgtcatggat tttggctgta tttataaggg atattgttca
    18961 gatatgacca gaacggttgt gattggcaag gcaagcagcc gtcaaaaaga aatctatgat
    19021 attgtatttc aggcacaaat ggctgctctt gaaggtcttg gtccttttaa aagaggaaaa
    19081 gaaattgata agatagccag agattatatt gaaatgaaag gctatgggtc ttgttttggt
    19141 catggtttag gtcattgtgt gggtatggaa atccacgaaa atccaagatt atctaagaat
    19201 gaggaagaag aatttttgcc tggtatggtg gttacagtag agccaggtat ctatattcct
    19261 gattttggtg gcgttcggat tgaagacctg gttcttatta cggatgatgg tattgaaatt
    19321 ttaacgaaat cccctaaaga acttatagaa atttaaaatt aataattgaa aaagttttca
    19381 ttttgtttta aactaaacaa ggtgtaatat atcgaattta tgattgagga ggaaatatat
    19441 ggtttctgca ggagatttta gaaatggatt aactattgaa ttagaaggag aaatatatac
    19501 agttgttgaa tttcaacatg ttaaacctgg aaaaggagct gcatttgtac gagcaaaatt
    19561 gaaaaattta aaaacaggtg gaattattga aaaaactttc agaccaactg aaaagttacc
    19621 taaagcccat atagaaagaa aagatatgca gtatttatat aatgacggtg atttgtatta
    19681 ttttatggat tctgaaacct atgatcaaat tgccttaaac aaagaccaac tgggtgattc
    19741 cttaaagttt gtaaaggaaa atgatatggt aaaaatctta tctcacaacg gtctggtatt
    19801 tggtgtagag cctccattat ttgtggaatt agaaatcact cataccgaac ctggttttaa
    19861 aggagataca gcgactgggg caacgaaacc tgcggtagta gaaactggtg ctacaataca
    19921 ggttccttta tttgttgaaa ttggggataa ggttaaaatt gatacccgaa ctggagaata
    19981 tttaagcagg gtttaattgc tttacaaatc tcttaaaaag ctgtgcactt atttattagt
    20041 gcacagcttt ttttgttcta aacagggaca tcccctcata gaaatatata tagacaggtt
    20101 aaaggagggg aaagtttgaa aacaaaagaa gttattctaa gctttcttag ttccaggata
    20161 aaaagtttgt tctatcatat accagaagaa gaatttgaaa agatatacga aataagactg
    20221 agagtaaata agcccctgat tcttttaaaa gaaggtcaat attattttgt aaggagcgac
    20281 ggctatttaa caaaaaagct tgaacaagct tttattgtct ttcaaaagga tattattgat
    20341 accgcacaac ttatgagtga ttattcgatg tatgctttag agcaggaatt aaaaaatggt
    20401 tatattacgc ttcagggagg acaccgcgta ggaatcacag gtaaagttgt tatcgaaaaa
    20461 ggggtaataa aaaccattaa atatttcggg gctatgaata tcaggatatc tcatcaaatc
    20521 atagattgtg ccagagaact ttttccccat atcattaatg gtgaaaatat ttatcatact
    20581 ttgattattt ctccgcctgg ctgcggtaaa accacgatgc ttagggatct aatccgctta
    20641 gtcagtgatg gggttgaaaa tcttttttcg ggtataacgg taggcgttgt cgacgagcgg
    20701 tcggagattg gggggtgtta tcaaggtatt cctcaaaata atgttgggat taagaccgat
    20761 gtgttagacg gatgtcccaa agcagaagga atgaggatgc tgttgcgctc catgtctcca
    20821 cgggttattg ctgtcgatga aatagggaaa atggaagata tttttgctat tgaagatgta
    20881 atgaatgcag gggttaaatt aatttgcact gttcacggca gttcacttgc agacatacaa
    20941 agaaaaccag tgctgagcga attaattaac aaaaaaatct ttgaaagatt tgttgtttta
    21001 agtaaccgaa aaggaccagg aacggtagaa gatattatag acggtattaa tctgaattca
    21061 ctgatgaaag gaagagaact ttgattaaaa tgacaggggc cttgatgatt ataacgggaa
    21121 cggcttttat tggttacctt tttgaccgtt ttgaattata tagaatgaaa gatttagaaa
    21181 acataaagag agccctgtta tttttaaaag gagaaataga ttatatgatt gcttctttgc
    21241 cagttgccat ggaggaagtt ggttcacggg tgggaacgaa tataggtaaa attttcagcc
    21301 atgtcggaga aagtatgaaa aataaaaggg gttatagtgc agagaagtta tggaaggagt
    21361 cggtagaact ttataaagac agtacttatt tatccaaaga ggatattaat aatttattgg
    21421 ccctgggtac tgcccttggc tatctggata aagaaatgca aaaaaacaat atcgacttac
    21481 tactggttta tttagaagat gaaatcaaca gactaaaact tcaccgtcat aaaaatggca
    21541 gattatataa gactttaggt tttttaggag gaattttgat tgtgatacta ctttattaaa
    21601 ttgtttacaa aaggggaggg cttttatgga cattacgatt gtatttaaaa ttgctgcggt
    21661 gggtattctg gtctctgttt taaatcaggt tttggtccgc tcaggaagag aagaacaagc
    21721 catgctgact actttagctg gtcttattgt tgtgcttttt tggattattc aatatataag
    21781 cgatttattt aaaactgttc aaaccttgtt tcaattatag gggtaattgc tatggaaata
    21841 ggccaaattg ttattattgg actgatttca gttgctttag ctttgatgct ggaaaaacac
    21901 aatccggagt ggagtatgta tataagaatt gtcgcaggaa tattgatatt ttttattatc
    21961 attcaaaagc tgggaattct tttggaaatt gcaaaaaaaa tgagtgaaaa aatagatatg
    22021 gattttgttt atataggaat agtctttaaa gtattaggta tttcatacat atcagaattt
    22081 ggagcccaac tttgtaaaga tgccggggaa tcttccattg cttccaagat tgaatttgca
    22141 ggaaaagtat tgattattac cgtttcatca ccagtgatgc tggcgttaat tgatattata
    22201 tctgacatta taccataata ggagttgtga aatgaaaaaa gtaaagatat tgttaattgg
    22261 tttattatgg ctgtttatgc ctgtaaaagc ttattctcag tctgtggatg aaatttggac
    22321 aagacaaatt gaaagtttag acttttccag aatcgatcaa atggtaacaa acattaaaag
    22381 ccaaaaagaa aatcctttct tgcagggatt tgatttcaag gatattgtca ttaaaagcat
    22441 taaaggtgaa ctggattttt ctttaggcaa tattataaaa agtataatat ttttattctt
    22501 taaagaaatg ccggcatatt ttagtcttat agggaaatta atggttattt cgatgctcag
    22561 tgctttttta aatagcttgg cagaatcttt tcaaagcaaa tctacttcaa atgtcgcttt
    22621 ttatgtttgt tacatggtat taattatact gctactgcaa tcgtttcaga ttgctataca
    22681 gctttcacag tcgactcttg aaaacatggt cctgatcatt caggcttctt tgcctgccct
    22741 tttgacttta atgatggcat ctggggaact gacaagtggg gcagtatatg agccggtttt
    22801 actatttgct attcaaatca tgggtgttct tattaaaaaa attttacttc ccttcatttt
    22861 ttttacagca attttgtcag taattaattc gctttcagat aagaatttat taaaaaaaat
    22921 gctggaatta tttaatcaag ctatcgactg gttgattaaa ggaattgcca tcttatttat
    22981 tgctctaatg agcttaagca gttttacagc accggtatta gacgggatta ttaacagggc
    23041 tgccaaaaca gcagtaggag tggttccagt tgtaggggaa actttaacag gggcggttga
    23101 tattgtaatg aattgttctt tgttaataaa aaatgcagtg ggtgtgggcg ttattatact
    23161 tctggccgtt tattgtatat tgcccttaat taaaattttg ctttttttca tggtgtataa
    23221 attgactgca gcgcttatag aacccatatc ggataaaaga attgttaatt gtatttccga
    23281 tactggtgag gcatgtatta agttatttag ccttgtcttt acaagttctt tgatttttat
    23341 tgtggcaata acgattttaa taggcatagg cagtatgact ttaaactaaa tttttgactg
    23401 ggagggtaaa atgattgatt atttaggaaa ttggcttaga aatatcgcct tttttataat
    23461 ttttactgtt tttttagaga tgctcatgcc tgatggcagc tttcgaaaat atatccgaat
    23521 gcttttatcg cttattttaa ttttaatcct taccaggcct gtgggctctt ttttattaga
    23581 agcttcttct gtcgccgacc ggaccatcat taatgaactg gaatttgaaa gacagtccat
    23641 cctgaaagaa acaatcgttt tccaggaaaa acaagatgac ttaattattg aaacttataa
    23701 agaaaagctt gaaatgcaaa tcataaaatt gattgagagt aatagtcact atagtcccca
    23761 ggttatagaa atccaaataa acgaagacag aagttctcaa aatttcgggg cgattgaagc
    23821 aataaaaata ttgcttacag aaaaagaaga tcataatgcc cgggacaata ctattggtgt
    23881 tgtaaaaccg attcatatca atacaagtat aaaaaacgaa aacaagacag aaaattctaa
    23941 tattaatccg gaaatagaaa aaaatataaa aaatttatta attaacttct ataatttgtc
    24001 cagtgataat atacatatta ctgtacaaaa gaaatattag gggtgggaac atgggaaaaa
    24061 tcatggaatg gttaaaatta caggataaaa aaaataaaaa tctcttatac aatattacct
    24121 tttttattgg gattggtgtc ttattaatca ttcttggcga tacggcgttt aataatcttc
    24181 ctcgaaaatt agaagaaaag agggataatt tagtaagcaa tgagggaaac ttaaaagaaa
    24241 tgaataagac atacgaagat gtcatagaag aaagattgaa agagatattt tctaagatgg
    24301 aaggtgtagg aaaagttgaa gtaatggtta caatatctta tggtaaagaa atatttcttg
    24361 cagaagatgt caattcaaat tattcaagaa ccattgaaga ggatagccag ggaggaaaaa
    24421 gagaaataac gagtgaagat atacaaagta aaactgttat gcaaaatacg ggctcgggta
    24481 gtacacagcc agtcatcttg aaagaaaagt atccggagat aaagggggta ttaattttag
    24541 cagagggggg agaagatcct agagttaaac aacagctgac agcagctgct gaaactttat
    24601 taggagttcc tgcacataag gttcaggtat ttaaaatgaa taaagaatag ggggaattct
    24661 gtatgttcgt tttaaaaaga aatcaaatca ttgttacggc tttagttgct atgattgcta
    24721 ttgccggcta tttaaattat acagacagtc agcaggttaa agatgcagac attgtgtata
    24781 atgaaaacct ggatataacg gataccattc ccgatgatgc aatgattatg ggagaaatgg
    24841 atctgacgga cgaaatggaa gaaggaatta ttggagaagc tatatcaacg gatccagaaa
    24901 tcttagctca aattaattct gctgatttag gagaagattt cgatagcatt gttgagacag
    24961 ctgcgggaga agagtcagcc gtcgcagatg aaggtgaaga tgatggggct gctgtttttg
    25021 tcaacagtag tacggataca ccttattttg cccaggcaaa gctcagcagg gagcaagcaa
    25081 gggcgaaaga aagagaaatt ttatctgaaa tcttaaataa taaatccctg gacaaagaaa
    25141 agaaatccca ggcagcagat gaaatgttaa agctgcaaag gcggattgaa aaagaatcag
    25201 ccatggaagc tatgattgaa gccaaaggct ttaaagaagt ttatgtaaga attagcgatg
    25261 acagggttga tgtagttgtt aataaagaaa agttaactga acaagaactg gcacaaattg
    25321 aaagtatcgt ccgctcaaca acgggatacg atgttgacaa aattcaaata attcccctca
    25381 aatctcacta aaagcaattt gcaaatagaa ataaatctgt tataatagtg taaaagtacg
    25441 tactttatcg tgtgaaagtt taagttatgc ttaggtagga ggaatttaaa tggacgaaag
    25501 aaacgtttct tcaatggcag aaagcaacca aataggtcaa attcagattg ccgatgaagt
    25561 gattgcaatt attgccggtt tagcagcaac agaggtagaa ggtattgccg gaatggcagg
    25621 ttcttttact ggaggtattg cagaaatttt aggcaagaag aatttatcca aaggggtaaa
    25681 agtagaagta ggagagagtg aagtttctat tgatttattc cttgttgtaa aatttggcgt
    25741 aaagatacca gaggttgccc tggctgttca gaaaaaagtt aaaaatgcag ttgaaaccat
    25801 gacaggtctt tcagtaatag aaattaatct tcatataacg ggtgttcatt ttgataaaaa
    25861 taaagcagat aatacaaatc aggaagcgga attttttgaa taagaaaata agaaaaggag
    25921 aaattaaccc ttaaatatgt tatatattta agggttaatt taagcaaatt tacaaatgca
    25981 tttactagga ggaaaaaaat gagccgccgt agagccagag aacatgtttt catcctctta
    26041 tttcaaatga cttttattaa tcaagaggaa acagatgaaa gaatagaatt atattttgaa
    26101 caacacagtc atattaaaga agaagataaa aaatatatta cagaagagct taaagcagtt
    26161 ctttctaaat tagaaactat tgatgaattg atttcacagc attcaaagaa atggaaaata
    26221 gaaagaatgt cccgtgttga cttgtctatt ctcagacttg ctgtatttga aatgcaatac
    26281 agagaagaca tccccaacag cgttgccatt aatgaagctg tcgaattagc taaaaagtac
    26341 agtggagatc agtctccgtc ttttgtaaac ggagtattag gaagtattgc ttcatcttta
    26401 ggtgagcctt atgatgaata aaaaagtttt aacggtatct gatgtaaacc attatattaa
    26461 agccttaatt gatagtgatt atgttttgag caatatctgg gttgaaggag aaatttcaaa
    26521 ttttaaaaga catagttctg gtcatatcta ttttactttg aaagataaag atggagccat
    26581 ttcatgcatt atgttcagaa gtagtgcagc attccttcgt tttgatcccc aaaatggtat
    26641 gaatgttatt gtaaaaggtt ctgtctctgt gtatgaaaaa acagggcagt atcaaattta
    26701 tgtcagtcag atggagcaaa aaggtattgg aactttatat gaggcttatg aaaggctgaa
    26761 agaaaaacta gaaaaggaag gccttttttt gcagtcgcat aaaaaagcta tacccgcttt
    26821 tccagaggtt gtagggatta ttacttctcc tacgggggca gcgatcagag atattatcca
    26881 aatatccaaa agaagaaacc ctaatatcaa attggtggtc tatccggttt tggtgcaggg
    26941 cgagcaggct ccgggggaaa ttgcccatgc catttatgag atgaatcagt ggggacaagc
    27001 cgatgttctt attgtaggaa gaggcggagg ctctctggaa gatttatggg cattcaatga
    27061 ggaaattgtc gcaagagcta tctttgaatc aaagatacct gttatttctg cagtaggcca
    27121 tgaaactgac tatacgatag cagattttgt tgcagatttg agagctccaa ccccttcagc
    27181 agctgcagaa ttggctgtac ctttattaaa ggacttaaga ttaagggtgc acattgctta
    27241 taaaacgctt aatcatttaa tggaagacaa aatctattca gaattagaaa aattaaagaa
    27301 gattaatcaa tcggttcctt ttagaaagcc tttagacagg atttataaaa agcagcagga
    27361 cttagatagc ctggaaaaaa tcctgaataa cagtatgaag cacattttag aaaaaaaaag
    27421 gagccagttt ggtaaagaaa taaaagcttt agaagcttta tcccctttga acactttatt
    27481 aaggggttat tccattactt taagcaaaga aggccggaat atttcttcaa taggggatgt
    27541 caaagtggaa gatgctattt atataaagtt aaaagatggt atcattgatg cagttgttaa
    27601 aggaattcag gaagatgagg aggggtttga tggctaagaa aaagccctta tcttttgagg
    27661 aaggaattaa gcgtttggaa gaattggttg aattattgca ggaaggggat attcgtttag
    27721 aagacgcttt aaaatattat aaagaaggaa tggaattaag tctgctttgc tctaaacagt
    27781 tagaggaagc agagaagcag gtggttgagt tgcaaaaaga ttttgaaggt aaatttgttg
    27841 aaaaagtatt tgatgcatgg gagaaagaca atgaactata aagaagcatt agagcaaaaa
    27901 ataaaattaa tcaatcatta tttggacaaa ctatttaata gtattccaga ttcacaggct
    27961 aaaatcattt atgatgccat ggcatatagt atttttgcag gtggtaaaag acttagacca
    28021 gttttaatgc tatcagcttt tgaagcggtt ggtggagaag gtgaaatcat ttatcctttt
    28081 gcctgtgcca ttgaaatgat acatacctat tctttaatcc atgatgattt acctgccatg
    28141 gacaatgatg atttacgaag aggcaaacca accaaccata aagtttatgg agaaggaatg
    28201 gctattctag ctggtgatgc gcttttaaat aaagcatatg aactgatgat taaggcttcc
    28261 ttgacttgtt caggaagaga agcgcttgaa gcaatggata ttattgcaac ttgtgcagga
    28321 acagagggca tgattggcgg acaagttatt gatttgcttt cagaaggccg tcaggtggat
    28381 atggaaaccc tgctttttat tcataaaaat aaaactgcag ccatgataga agcttcatta
    28441 aaagctggtg gaatcttagg caaaggaact aaggaggaga tagaggctct gggacagctg
    28501 ggttattgta ttggaatggc ttttcaaatt caggacgata ttttagacat tacaggttca
    28561 caagaaaaat taggtaaagc tataaacagc gatataaaaa atgaaaaagc aacttttgtt
    28621 tccatcgtag gtcttgaagc gtcaaggaaa tatgtagaaa agcttaccaa acagggaatc
    28681 agtgctttgg aagttttcgg agaaaaagct gcttttttag cctggctgtc ggattatttg
    28741 atgacacgta cgtattaagg aggatttatt ttgcaactta ttcaagctat atggaataat
    28801 aatgttttaa aagctgccgt agtcgcttgg tttatagcac agttattgaa agtaattgtt
    28861 gtattaatac agagccgtaa gcttgatttt tccagattgg ttggttctgg aggtatgccc
    28921 agttcccatt cggcttttgt aatggctatg tgtgtaaacg taggggaaac tgtcggttat
    28981 gattcgcctt tgtttggtgt tgcagttgtc ataggattta ttgtgatgta tgatgcagca
    29041 ggtgtaagaa gggctgctgg aaaacaggcc gctgttttaa ataaaattat tgaagaaata
    29101 gaaaatatgg attttcattt tgaagaaagg ttaaaagagc ttcttggtca tacacctgtt
    29161 gaagtgattg cgggggccct tttagggatt gtaatcgggt taatattttg ctgacaggaa
    29221 agaagggatt aatctgcaat gattatggat ggcattcatt cacctaaaga tttaaaaaag
    29281 ttaaatgcag ataaattaaa catccttgcc aaagaaataa gagccttttt aattgagaaa
    29341 attagtcatt ccggaggtca tttagcttcc aacctgggtg ttgtggagtt aactttggca
    29401 ttgcattatt gttttgattc ccccaaggat aaaattattt gggatgttgg tcatcaaagt
    29461 tatgtccata aaattcttac aggcagaaaa gatcaatttg accgtttaag aaaatataaa
    29521 gggataagtg gttttccaaa acataaagaa agcatccacg atatttttga tactggtcat
    29581 agttctacat caatttctgt agccctgggg cttgctgctg cccgggattt aaataatgaa
    29641 aaattttctg tgattgcagt cattggagat ggttctttaa cgggaggaat ggcttttgag
    29701 gctttaaata atgcaggcag atccaatagt gatttaattg ttattttaaa cgacaaccaa
    29761 atgtcgattg cgccaaatgt gggaggactt tcaaaatact taaataagtt acgtacagaa
    29821 ccagtttatc ttgaagtgaa agaagatata tcccaggctc ttaagaaaat ccccgggata
    29881 ggggaaagcc ttgaaaaaac tgtgaaaaag acgaaagaag gtattcgcta tttactggtt
    29941 ccaggaagcc tgtttgaaga actgggcttt acttatgttg gtcctgttga tggtcacaat
    30001 attaaagaat tggttacggt aattcaacgg gttaaaaaga tgaaaggacc tattttactt
    30061 catgtcaata cggtaaaagg aaaaggctat agttttgccg aaaagtatcc cagccgttat
    30121 cacggtgtag atacctttga tatagaaaca ggagaatctt taagaaaaga caataaagaa
    30181 agttattcag atgtttttgg aaaaacctta gttgaactgg ctaaagaaaa taagaagctg
    30241 gttgccatta cagcagcaat gcctgatggg accggtctaa aggagtttag taaaaaattt
    30301 ccccaaagat tttttgatgt aggtattgca gagcagcacg ccgtgacttt tgcagcaggt
    30361 ctggcaaaag ggggctttca tcctgtattt gcggtatatt cgtctttttt acaaagagct
    30421 tatgaccaaa ttatccatga tgtctgcatt caaaatttac ccgttgtttt tgctattgac
    30481 agggcaggta ttgtaggacc tgacggagaa acccatcagg ggatatttga tttgtctttt
    30541 ttatcccata tgcctaatat gaccattatg gcaccaaaag ataaaagtga atttgttgaa
    30601 atgctgaagt attcttttag tataaatgga ccagtggcca tccgttaccc taaaggggaa
    30661 gcgccagatt tgttcccttc agtgcatcaa cctattgaat atcaaagagc agagattttg
    30721 tatgaaggaa aagatgctgc aattattgcg gttggtaaaa tgatggaaaa ggctaaaata
    30781 attgttgaaa atttaaaaga aaaaggctat catttaactt tgatcaatcc aagatttatt
    30841 aaaccagtgg atgaagaaag tattgtcgag cttgcgaagc agcataataa aatttttact
    30901 atggaagata atgttttatc cggtggtttt ggcagtaaag tgctggaatt gcttgcaaaa
    30961 caccatataa aagatgttga ggtcaggatt tttgcttttc ccgatgaatt tattgcccag
    31021 ggaagcgttg aagaattata cagggaatat ggactggatg ctgaaacaat ggaagaagaa
    31081 attattcgtc aattaacggc taaaggatga ttatgtaatg agcagcaaag aaagattaga
    31141 tttacttctt gttaaaaaag gtttttttga ttcaagggaa aaagccaaaa gaagcattat
    31201 ggcaggttta gtatttgtag atggtatcag agaagataag gctggggcaa aagtgagtac
    31261 agaggcccgg ttagaagtca aagggacaat gaaatatgtc agtcgtggag gttataaatt
    31321 agaaaaagcc ctggattgtt ttgcaattca tttagaagat aaaattgctt tggatatagg
    31381 ggcgtctact ggaggtttta cagactgtat gcttcaaaag ggagcaaaaa aagtctatgc
    31441 cattgatgtt ggctatggac aatttgcctg ggagcttaga aacgatgaac gggttgtttg
    31501 catggaaaag actaatatcc gttatgtcag ggaagaagat ttagatgata agggagattt
    31561 tgcttctatt gatgtgtctt ttatttctct ttgcaaagta ttaagacctg ttatgaattt
    31621 attgaaagaa gaaggggaaa ttgtagcctt aataaaacca cagtttgaag ctggacggga
    31681 aaaggtgggg aaaaaaggtg ttgtcaggga tcctaaggtc catgaagaag tgattatgaa
    31741 tgttattgaa tatggcttaa gtgaaaattt atatttgaaa ggtttaaccc attcccctat
    31801 caagggacca gaaggaaata ttgaatattt agtttatttt gttaaaaata gcaataatca
    31861 tatatatcat gcggatcatc aacttattag agaagttgta agaaaagctc atgaaacgtt
    31921 aaatggataa ggatgggctt ttcttataaa gggagggatt tttcttgaaa aaagcaggcc
    31981 ttatcgttaa tataaaaaag gatattaatc tggatagtag tataaaaatt atggattggc
    32041 tgatgcaaag aaattgcttg gtttatatcc ctgaaaatct ggcaaaaatt ttagaaaagc
    32101 ctcatctgtc aaaaaaagaa gaagaaattt atcaggattc agattttatt gtggttttag
    32161 gaggcgatgg tactttttta agtgctgcca gaaaagcggc aatttatgat acacctattt
    32221 tagggattaa tttaggtact ttaggttttt tggccgaagt agaaaaagca tcctcccttc
    32281 aggctttaga aaaagtcctg gccgggaatt attctattga gaagagaatg atgctggaag
    32341 taaaggtaaa ggattgtaaa aaaaataacc agcagaaact aatttgttta aacgatattg
    32401 gggtgacccg gagttcctta tccagaatta ttaatttaaa agtatacatt aacaatcagt
    32461 ttgtggatta ttattcagct gatggtatca ttgtatcaac tcctactggt tctacggctt
    32521 ataatttatc agcgggaggt cccattcttg atcctaattc taagatgatg gtaataacgc
    32581 ctatttgtcc ccatagttta agtgcaaggt ctattgtcgt ttcagatgaa gattatatag
    32641 aaattgagat ttgcgaaaac cacaactgtg atattatcct tacgatagac ggacaattag
    32701 gctatcaatt agataatcat gatatcatac aggtaacgaa atcaacttat atgacaaaaa
    32761 tcattaagac ttcggactat aatttttatg atatattaag gaagaaaatc gttgggataa
    32821 ggaagtgagt agatgaagct taaacgtcaa gccaaaattc ttgaactcat taacaattat
    32881 gatatagaaa cccaggagga attggcggac agattaaggg aagcaggatt tgaagtcact
    32941 caggctacag tttcacgaga tatcagagaa ttaaaactta cgaaagttgc taacgagaaa
    33001 ggcattcaaa agtattctgt aattggccaa agtgaaaaga atatttcctc taaatatatc
    33061 cgggtattta aagaaggctt tgtcagtatg gacattgcgc aaagcatttt ggtggtgaag
    33121 acaataacgg gaatggctat ggcagtcgca gcagcgatgg atgcatttaa ttatcctgaa
    33181 attgttggga ccattgcagg agatgatacg attttttgtg ctgtaagatc cgaccaggaa
    33241 gctttgaagt taatggaaaa actaaaccgc atcctgaatt cctgatagga gttgattttc
    33301 atgcttgtac atatctatat aaaaaacata gccttaatta atgaaataac cattgatttt
    33361 aaagaatttt taaacatttt aacaggggaa acgggcgctg gaaagtctat tttaattggt
    33421 tctattaatt ttgctttagg agagcgtggt tcaagagata ttatccgtac cggtgaagaa
    33481 aatgcccttg tggaacttct tttttttgtt gaaaatgatg aaatcctgga gcttataaaa
    33541 aatcagggaa tagaagtaga agaagaccgt tatgtcctta ttacaagggc gattaatcaa
    33601 tcgggcagaa ctgtatgtcg gattaatggt caaacggtaa caactggcat gttaagacaa
    33661 gtatcttcta aattgataga tgtccatggt cagcatgagc atcagtcctt attggatgtg
    33721 ggaaaacata tagaactatt ggattctttt tgtaaagatg agttagcggc tttaaaaaaa
    33781 gaactggaaa gcaaatataa agaatataaa aatattgaaa aacaaataag ttccctgata
    33841 ggtgatgaaa aagagattga acggaaaatt gatcttctgc agtatcagtt agatgaaatt
    33901 acaaatgcaa aatttgaaat tggtgaagaa gaacaactcc aaaatcaatt aaaaaagctg
    33961 tctaattccg aaaaattagt aagaggcatg gaagaacttt atggcttatt atatgggaat
    34021 gattatgaaa atcattctac ctccttattc ttatctgaag cgctgaggct tttaagggat
    34081 ttagcagatg tcgatgatga actgatgcct ttatatgaaa atcttgagaa tatacagctt
    34141 ttactggatg acatggttag agatatcaga cagtacaaag atcagatcca ccatgatcca
    34201 tatgaattaa aagctgtaga agaacgtctg gatttattgt ttgatttaaa aagaaaatat
    34261 ggaaattcta tagaggaaat tcttctttat aaagataaaa ttgaagaaga attagaacgg
    34321 attgttaata gtgaagaaac cttaaaaaat ttaaaaaatc aatatgccct tttagaggat
    34381 gaaattaaaa gactttcagg aaaaatttct gacatccgta aagaaaaagc caaaattatt
    34441 gaagaaagaa ttgaaagtat cctggctgaa ttagcaatga aagaagctaa gttttctgtc
    34501 atgtttagcc aaaaagacag ctttaatgaa aatggttggg ataatgttga atttttgatt
    34561 tctacaaacc ctggtgaaga attaaagcct ctggctaaaa ttgcatctgg tggagaaatg
    34621 tcaagaatta tgctggcatt aaaatgtgta cttgctgata ttgatgaaat aggaacattg
    34681 atttttgatg aaattgatac aggaattagt ggggcaacgg cacaaaaagt tgctgagaaa
    34741 ttaggttaca tatccagaaa aagacaggtc atctgtatta cccatttgcc acaaattgct
    34801 gccatgggcg attgtaatta caaaattgaa aaaagaattg acgaaaacaa ggcaatttct
    34861 tccattaaag cgttaaaaga agatgaagta atttatgaat tggcacgact aatcagtggt
    34921 gcacagataa cggacattag tttaaaaaat gcagaagaaa tgagattgct ggcaaagcat
    34981 tttaaggcaa agctgaatta attttcttgt aagcttttga ctagtataag aacaatttaa
    35041 aaaggttaat ttatgtttat aagctacatg ctccagaaac ataaattaac cttttttaat
    35101 ttgtattgag ggtgaaaatg atgaataata aaaagttttt aagatttgct gttttatttg
    35161 ttatttttgt acttttgatc agtccttatg cagcttctta ttattatata ccaaaagaaa
    35221 taaaaatgat ggcagggaga aatcatgatt ttgaatttaa tctcccgctt caggcgaaaa
    35281 tttcatcaga aaaaataggt gttttaaaat ttaataataa accaatagaa agcaataatt
    35341 ttcaaattga tttacgttct cctttttctg ttcagccaac agaacaagca acgcttgatg
    35401 taaaattaaa atttttagga atagttccac taaaaaccgt aaaagtcgat atcatatctc
    35461 aaaaagaatt ggttccttgc ggaatgaccg taggcgtaaa cgtatctact gatggcataa
    35521 tggtcttagg gacaggaaca gtcatttcag aagatggtaa agagtatacg ccctccaggg
    35581 gcatacttaa aacgggagat ttaattttaa aagtcaatga aaaaagtata gaaaataaag
    35641 aag
//
LOCUS       DAOSUD010000014        33916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_894_length_33916_cov_23.960131, whole genome shotgun sequence.
ACCESSION   DAOSUD010000014 DAOSUD010000000
VERSION     DAOSUD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 33916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33916
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_33916_cov_23.960131"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..981)
                     /locus_tag="PLG49_03365"
     CDS             complement(61..981)
                     /locus_tag="PLG49_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ16388.1"
                     /translation="MTLQQIKYVVTVAETNSMNEAAKRLFISQPTLSSAIKDLEAEIN
                     INIFTRTNKGVILTAEGEDFLGYARQVLAQMELLEEKYIEGKKIKKKFGVSTQHYSFA
                     VKAFVEMVKEFDMNEYEFAIRETRTADVISDVRNGKSEIGILYINDFNEKVINKLLKE
                     NQLEFHDLIQCKGYVYLWKGHPLARKDKISMEELSDYPCLSFEQGDNNSFYFAEEILS
                     TYDYKKTIKACDRATMLNLMVGLNAYTLCSGIICEELNGSDYCAIPLDIDVNMNIGYI
                     TRNQGVVSHIGMRYIEKLKDYIDYYVKTTV"
     gene            complement(1094..2404)
                     /locus_tag="PLG49_03370"
     CDS             complement(1094..2404)
                     /locus_tag="PLG49_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOQ16389.1"
                     /translation="MSKKKYADRKFRFETLQLHVGQEQPDPATDARAVPIYQTTSYVF
                     KNCAHAEARFSLQDVGNIYSRLTNPTVDVFEKRIAALEGGIAALAVASGAAAITYTFL
                     NLAQAGDHIVSAKNIYGGTYNLLAHTLPSYGITTSFVDPFNYKEIEEAIKENTKAIFV
                     ETLGNPNSDVVDIEKIAAIAHNYKIPLVVDNTFATPYLIRPIEYGADIVIHSATKFIG
                     GHGTTLGGVIIDSGKFDWEGSGKFPSLTEPNPSYHGISFTKAVGAAAFVTKIRAILLR
                     DTGATLAPFNAFLLLQGLETLSLRVERHVENALKVVDYLKNHPQVERVNHPSVSQDPQ
                     QSELYKKYLPDGGGTIFTFEIKGDEQKAKDFIDNLELFSLLANVADVKSLVIHPASTT
                     HSQLTKEELLDQGIKPNTIRLSIGTEHIDDIIEDLEEAFKAIAE"
     gene            complement(2671..3798)
                     /locus_tag="PLG49_03375"
     CDS             complement(2671..3798)
                     /locus_tag="PLG49_03375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOQ16390.1"
                     /translation="MAFVYGILAFILLFGSRIKTKKGSWHEDFLSLESTKAIQGFCAM
                     TIILHHLSQRLTEQRLLFPFSQIGVLLVGVFFFSSGFGLIRSWRTKENYLNSFFEKET
                     SCSSCFFLCYDHYLFAYYIGGARFSLSGLLLRLSGLILANSHAWYIIAIVIFYIAFYL
                     IFKYIKNEKAAFTLMGIFCYITVSLLLGHSQENLWLHGEWWFNTCPLLFVGMLVARFE
                     DIIVNFVKKKYSIILIVFILLFVLFFAASLYTLSKYSYWAELKGGSGIPERWICLAFQ
                     VPAIVFFVFTVFTLGLKLKCSGPVLKFLGTMTLELYLIHNLLIGLFVDFIPIKSDILY
                     VYLVLLFSILTAYSVKKLDDFLIKILNKKFRGVQHEKVPFN"
     gene            complement(3958..5106)
                     /locus_tag="PLG49_03380"
     CDS             complement(3958..5106)
                     /locus_tag="PLG49_03380"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ16391.1"
                     /translation="MKRKHKFLWLIFLIFSLSACGQNVNTNSENESLLTQEEYLNVYG
                     DVKVDRSQEFVIDFPAKIETIFINDGDLVKKGDKLIALDLKDYLLEIQLKENEVQILE
                     SQLKKLSENSNPLMPDISRIQNELRIKENYLLTGEDPDLIPLKNRLEIIEKAIAISQQ
                     EYEANQELFEMGAITEKELKQSEQIYKSNLKDKEDTLMAIEKVKSNRRIEIDGLKTQL
                     ESISRQAANTDKQKLYETEELSIRIESAKLQVNAMKSKLNRAFIQDNYIVAPEDNLII
                     YDIYCKEGSRIGPEQALLKAMYQDTMMVIADIPEESISLVSAGAEAIIVPPYENGEEI
                     TGRIRTISNRAVEKDGDIYLEAIIDIEKGKELLKPGMTVDIKIPANID"
     gene            complement(5129..7165)
                     /locus_tag="PLG49_03385"
     CDS             complement(5129..7165)
                     /locus_tag="PLG49_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ16392.1"
                     /translation="MEKRYNLLIEEVKYIDFNFNKAEKSKEEKVLRIFSASREINQLA
                     IVEGKPVTSKDEIVMDPKFAKENNYQIGDSISIRDSHFKIVGYGITPDYVYVLKNTSD
                     FLTSPQTFGVAYLNEEGFERIKTNESKSTLYSYKKMGNSIEPLKKYLRDHTILLDFLE
                     KKENSRIETIFDDSEGPKQMALVIGLLLVVIVAFIISISIQNTIQEESQTIGILYAQG
                     INKREMIKHYMILPVLLVLIGTFIGYGGGVLLSEPIIILQRTEYTVPDVIVKDSFYLI
                     FIGIILPITLAVAITYFYLSKTLDKTPLSLLRGEYSNKKVSKLERICTFNSLHFFPRF
                     RLKGMIRERRSMLALLIGVLLAVTVLMASAYIRDSCIVFVEELIDKVPFNYIYTFNDP
                     GDLHKYSKQGELTTIKPIKVIINQEKKNFTIQGIKPDSKFFKSEFLKDLKENEVLISS
                     SILTKFNLELGDRLLLFDDIENKYYEVVIKDTSEYDYGQYLYTNIYIFNKIFNIRKDS
                     YNALMTYEPLDIAEEKINSVTVKNDMIKGIGNLLGMINVMSGIMLVAAVCILITIIYL
                     LMNMVINKSKTNISMVKIFGYSQNEISQMYMRGTYLFLLLAFFGGRPLGYIITKNLYD
                     GILADMQQYVMVRIKNLSLFSSFIIMSLAYFITCHFLRKNLDKVALTEALKNRE"
     gene            complement(7255..7782)
                     /locus_tag="PLG49_03390"
                     /pseudo
     CDS             complement(7255..7782)
                     /locus_tag="PLG49_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(7824..8411)
                     /locus_tag="PLG49_03395"
     CDS             complement(7824..8411)
                     /locus_tag="PLG49_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ16393.1"
                     /translation="MGSAFTEEEKKIIREQLKKAAREYMIQYGIKKTTVDDLVQKVGI
                     SKGAFYKFYGSKELLFFEVIEDFHEEFYAVALKVLKERTDLDLKDRVELAIWESIRYI
                     QEAPIMFHVKDEMPYLLRKVPEEILTEHEEANHQYVEEIIKAFGIKIAVPEEFIKAFM
                     NVLEILFIHQSSIGEKYFHEILKLFIKAFVNKVLE"
     gene            complement(8634..10331)
                     /locus_tag="PLG49_03400"
     CDS             complement(8634..10331)
                     /locus_tag="PLG49_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ16394.1"
                     /translation="MSKRILIVGGVAGGASVATRVRRLDESAEIIIFERGPDVSFSNC
                     SLPFYLSGIVDNSEALVLMNPQKFKKQYNIEARTNSEVIKIKRAEKKIVIKNLLTGEE
                     YEEAYDKLVLSPGARPVRPKSIEGVDGSNVFTVRNVIDIKNLKDYIDNHHIQDIVVVG
                     GGFIGVEVAENLRLAGKNVSLIEAQGQILCPFDYDMVQILQKEMLDKGVNLILNDGVK
                     RINEAFVELQSGKIIKAKAVVLAIGVTPEVSLAKEAGLEIGETGAIKVDHNYLTSDKD
                     IYAVGDAIEVYHRLTHKKTRLPLAGPAQRQARAAADHIYNIPHRNNGVIGTSIVRVFD
                     LGAAVTGLNEKTAKEAGISYDFVYVIPKDKVGLMPDSYPIHFKLIYEYPTGKILGAQA
                     IGKGNVDKRIDVIATLILMGGTLEDLKELELSYAPLYGTARDVVNQAALVGLNLLNGY
                     FKQVPVSKVRELVETGAFIVDVRERTEYERGHLINAVNIPISELRDRIDEVPKDRPVY
                     IHCRSAQRSYNAVMLLQNLGYENVYNISGSYLGICYYEYFQDQVTGREKIVTEYNFS"
     gene            complement(10603..11250)
                     /locus_tag="PLG49_03405"
     CDS             complement(10603..11250)
                     /locus_tag="PLG49_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16395.1"
                     /translation="MNTKTSIKDIKASYLRLATVGYVLTIIVITVFLTVRGISLAGKF
                     HNEYMEQAHKRSQASLKILEKMGLEPRIDEVSDGIEFFLGYHDSEEYEYAPYYQDILP
                     LIKQNYLPDTAISYSEKFILYGTDTNLGGVSIKCVDINKDDGEYFYRLLNHLNDVYGC
                     KVIEKDLFDQIIQEYIEKQRDRKIAFEVDKYEVIYRMEGYKNHEFSISISKKIDY"
     gene            complement(11247..11960)
                     /locus_tag="PLG49_03410"
     CDS             complement(11247..11960)
                     /locus_tag="PLG49_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16396.1"
                     /translation="MKTFEYESSIFKASFIHIIIGLTFLIFIPIGLSIFFISFISWVF
                     LDYSTQVVLEALEELFFFINIIGDVDIITYVLVGTAVIFIPIFLISFYPRHYRIRRSY
                     IYAIDYENNNLYSFQHALASNVPMHFIGRHSSINKLPLGKIVTLVHNINNLKITINTL
                     KEIAEDNPMNLRIVATWYFEDFKIKKKNKKGLWLIANVINLNKDNDKKKRKKFFISSK
                     YNDYEELCSFIESKAVNSL"
     gene            complement(12074..12787)
                     /gene="deoD"
                     /locus_tag="PLG49_03415"
     CDS             complement(12074..12787)
                     /gene="deoD"
                     /locus_tag="PLG49_03415"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOQ16397.1"
                     /translation="MIPTPHIEAKDKNEIAKTVLMPGDPLRARFIAENFLTDVKQFNN
                     VRNVLGFTGNYKGRKISVMASGMGMPSMGIYSYELFKFYDVENIIRIGSCGAYTADLK
                     LYDVILVKDAWTESTYARVQGGYEKDIIEGSAELNERLKKYAEELNIPIREVRIHSSD
                     VFYRENPEAYKEINKKYGCEAVEMESFALFHNAKILGKKAACLLTVSDNLVTKEETSS
                     YERQNAFTHMMKIALEMAE"
     gene            13070..13633
                     /gene="ahpC"
                     /locus_tag="PLG49_03420"
     CDS             13070..13633
                     /gene="ahpC"
                     /locus_tag="PLG49_03420"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOQ16398.1"
                     /translation="MSLIGKEVKPFKAQAFHNGEFIEVTEQDFKGKWSVVCFYPADFT
                     FVCPTELEDLQDNYETFKSLGVEVYSVSTDTHYTHKAWHDSSETIKKITFPMIGDPSH
                     ILARNFDVLIEEEGIADRGTFIIDPDGVIQAIEINAGGIGRDAKTLIRKIKAAQYVRN
                     NPGEVCPAKWEEGAKTLKPSLDLVGKI"
     gene            13654..15183
                     /gene="ahpF"
                     /locus_tag="PLG49_03425"
     CDS             13654..15183
                     /gene="ahpF"
                     /locus_tag="PLG49_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOQ16399.1"
                     /translation="MILEPDIRNQLDQYLQLMENDIVIKVSAGSDTNSQDMLSLINEI
                     ASLSPKIKVEKAVLPKTPSFSINRMNEDTGIIFAGIPLGHEFSSLVLALLQVSGRPPK
                     IEAKVIDSIKNLKGEYHFESYVSLSCHNCPEVVQALNLMSLLNPNITHTMIDGSIFKE
                     EAESKDIMAVPAVFLNGEFFSSGRMTLEEILAKIGTSPDASELKNKEVFDVLVVGGGP
                     AGASAAIYSARKGLRTGIVAKRFGGQVLDTMAIENFISVKYTEGPKLAADLEEHVKNY
                     DIDIMKPYQVKSLRKNKFVEIELENGALLKSKTVIIATGARPRTLGVPGEAEFRNKGV
                     AYCPHCDGPLFEGKNVAVIGGGNAGVEAAIDLAGIVNHVTLLEFLPELKADAVLQEHL
                     YKLPNVTVLKNVQTQEITGTEKVNGITYLERDTGAVKHIPLDGVFVQIGLIPNTDWLE
                     GTLTLNHLKEIVVDKYGATNIPGVFAAGDCTDSPYKQIVISMGSGATAALSAFDYLIR
                     N"
     gene            complement(15220..15750)
                     /locus_tag="PLG49_03430"
     CDS             complement(15220..15750)
                     /locus_tag="PLG49_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16400.1"
                     /translation="MDYKLVYEVTQDIDFAWFFPFIMFIFLLFFPKIYVYCNIINNKE
                     QYNTEFSIRIIKIFSRICAVFVFMFCLLYVNNFIQEYKTIVIPYKEGNYKIVEGYVEN
                     YVPMPYEGRGHHESFEINHVKFDYEHYSVGLGYHRAGSHGGIINRNGQKLKIGYITCG
                     NENIIVRIEVPANEEN"
     gene            complement(16025..16786)
                     /locus_tag="PLG49_03435"
     CDS             complement(16025..16786)
                     /locus_tag="PLG49_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ16401.1"
                     /translation="MDLLYDKGNRINYLKAIEKRVSRRAYLNQKIEADKLERIREAIR
                     HYNKLSGLSIQLIEDGSQAFNGLRKSYGMFYGVRTLIALVGKTADPHIKEKAGYFGEL
                     LILEATALGLGTCFVGASYDKKNCPCQIGADESLICVITMGPVNEKPGLREKAIYHMT
                     HRKSIKLEELYEADMEVPSWFMKGVKAAQLAPSAINRHPVRFVYKKGEVYAYVKDTSD
                     FNLVDLGIAKANFEIGAEGKFEYGNYGRFVKTIKN"
     gene            complement(16818..17249)
                     /locus_tag="PLG49_03440"
     CDS             complement(16818..17249)
                     /locus_tag="PLG49_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOQ16402.1"
                     /translation="MYLQQIRCQDLMKKKKIILLIVVVIFVGFLILLNYLNDLNNYKE
                     KIASTRISPLDFREIPDGVYVGDYDVDFIYAKVEVTVKNGQVTDIKLLEHKNGRGQKA
                     EKIVEDIKAAQSLEVDTVSGATNSSVVIKKAIENALINAVH"
     gene            complement(17277..17912)
                     /locus_tag="PLG49_03445"
     CDS             complement(17277..17912)
                     /locus_tag="PLG49_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ16403.1"
                     /translation="MKESKKEVLAKFHRDNIIRASEKLFLKNGIENTSMDDIAREAGY
                     SKATLYVYFKNKDEIIGAITLLGMKAFLDYVRNCLMEHKDFKSQYFALCYALVDFQKE
                     HPFYFECVFSEINVDLELEETPKVYYEIYEVGEEINQEILKIFQEGVKEDLLRRDIQL
                     PLASFIFWASIYGIIRMSKEKNKYLQKGMGVSSEDFLQYSFELLYHSIKKE"
     gene            complement(18224..20959)
                     /locus_tag="PLG49_03450"
     CDS             complement(18224..20959)
                     /locus_tag="PLG49_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HOQ16404.1"
                     /translation="MDTSKKTAWHFIDDNGSFSLLAPHKNSYLYFPLVNDAGMMSSIT
                     PLLNGDIKAGQNEFFMLPVSSEDLHNSRSGRNFWLYIEGVGPWSAAGHSPGQIMDTFD
                     EEAEEVKLEAGFLWHKVIRENKKIGIQSEIINFVPATNDKVELMAVTIKNTGKDKMLI
                     TPTSSIPVFGRSADNLRDHRHVTSLLHRIYTIESGIEVQPALSFDERGHKENKVTYGV
                     VARDEEGNKPLGSFPLVEDFIGEGGSLEWPEVIVKNKDSFVYQGNIIEGYEAIGALRF
                     KEEILEPGEEKSYILALYIAEDRGRGEEIVRKYCSKENFYYYLNQNMDYWKQKLDVLQ
                     FYSADREFDLWLRWVTLQPILRRIYGCSFLPHHDYGRGGRGWRDLWQDCLALLMMEPE
                     EVRHLLINNYGGVRIDGSNATIIGTKPGEFIADRNNICRIWMDHGAWPWITTKLYIDY
                     SGDLDFLLEEQTYFKDSQAARSTEIDELWDKSQGNLLKTHKGEIYKGTILEHIMLQHL
                     TPFYNVGEHNNIRLEGADWNDAFDMAAQNGESVAFTALYGNNLAEISDMILILKEKKK
                     INTIELAQEMLVLLDTMIKPVNYDSVEEKKELLKRYFDACKHTISGEKVKVDLTALAR
                     DLKEKANWIKEHIRNKEFITNEEGYQWFNGYYDNHGRRVEGDFPEGVRMTLTGQVFTL
                     MGDIATGEQVKEVVKACDRYLKNPKIGGYRLNTNFKELKKDLGRAFGFAFGHKENGAM
                     FSHMAVMYSNALYKRGFSKEAYEVIQLIYNQCKNFEESRIYPGIPEYINEKGRGMYHY
                     LTGSASWLLLTVLTQIYGVTGDLGNLRLKPQLRREQFNHLNEAAVVTKFADRILKITY
                     VNTNGLEAGKYQIKRVWINQGEQKLIKPKSDYLIKREVIEALDKDKIHEIRVELA"
     gene            complement(20973..21593)
                     /locus_tag="PLG49_03455"
     CDS             complement(20973..21593)
                     /locus_tag="PLG49_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ16405.1"
                     /translation="MGKKNMNQIKKTDFYFDDKIMDYHFLFNSLETINCLALSIGASE
                     ICKVTLALANMFRFVINDNQATVLLKDEIDFTNEYLTIQAVRYNENLTFEIDSSHIML
                     TVMCPRFLIQSIVNRMIHYYIEKKDLVSHIKIAIEPKSNSVVVTIRNNLKDLQRNLLG
                     KKEEELLKEEILDIGKSSQADKNYDIKIINCDKTGITVKLELYSIF"
     gene            complement(21688..23445)
                     /locus_tag="PLG49_03460"
     CDS             complement(21688..23445)
                     /locus_tag="PLG49_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ16406.1"
                     /translation="MKYNFFHKMGIRSKLNIGYMTMMLVLVSVSFVMYIRINARQVER
                     KMFKTDQQQVFLIKTTIESVIYRVDMISADIALNPSVQKALKEGRKEYYYISEREINE
                     YLNNTINRDVDIASIYIIDKNNYIYYVDRDQKEARFPDDFLASLDTEELVSLKGRSKW
                     NIQKNMFIQDDNNYMTVSRAINSIEDYQTIGYVVITVRNNVLEDIYKKFSSNDMYSYM
                     IEDNKKQSIIFPYDGRAEEVKKTLSLIPYTVDEYYKKQIEKQTHYFIKVPLESIDWDL
                     SCAFVYDNNFQSIELIFIFLILLYAILSIIGSRIINSVILKPLESISSSINKIRDGDL
                     TTRFKITNSNDEINQLSAALNMMMDQINLLLEKLNKEHEIQRKLEFDLLVSQIKPHFL
                     YNTLNAVSAYISIGKKEEAFYILRTLAAYYRLCLSSGKDIITIRDELELLKNYVDIMN
                     IRMNKLFEVSYEIDDAVLDCTIPKLTLQPLVENSINHGIRDFEKALNIKCIIKKIEDK
                     NLIFIAVEDDGVGIPEKIIFKILGAEEVNKTSGFGLGSIIQRLSIYYKVEDINEIIKI
                     ESIEGKYTKVYLYIPIKNS"
     gene            complement(23468..25090)
                     /locus_tag="PLG49_03465"
     CDS             complement(23468..25090)
                     /locus_tag="PLG49_03465"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ16407.1"
                     /translation="MFKVMVIDDEPIIRTGLRHVVDWESLGFQIVAEAKNGIEALNKL
                     ENEAIDLIITDIKMPQMDGIELLKALREKDNPVKVILLSGYAEFAYAQKGLSLGAMDY
                     LLKPMDPVNIEEVLKKVHKRLTDEKKKEEHTQYLNEKLELTRSFLREKVISDIIHGKN
                     LDKLYTLIEEYDIPLTNHFVQVAIIELDDFEEQGEDWLLNGMYEEIADKVQTIIKQEL
                     EKENHITSIISMGGLGSINIIIQAKDISDKEQLDAIISKVLDKVLHKINNDLSLRITI
                     GIGKVYEKVELTYLSYFNAKESLKHKYVLGGNKIIHILQLDSHEQNKFIYPLEEEKIL
                     ISYILFGDSKSAKFLEELLDQIIKNNNYDLYKISLCFTQLATNIYQAVQDKYRYIKEI
                     YNLTEVKDLGFLKYKDIEEIKVHFINNVTELIHVIQKYGLNSNSTIVNMACEYVLEHI
                     DEKITLASISEYLHISKNYFCSLFKQETGENFLDYLTKAKMDRAKVLLKEDNYKIYEI
                     SDLLGYKEKGYFSKLFKKYTGFTPAEYKKNCR"
     gene            complement(25378..26214)
                     /locus_tag="PLG49_03470"
     CDS             complement(25378..26214)
                     /locus_tag="PLG49_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOQ16408.1"
                     /translation="MSFKEIKNQANINGCETARVKEEEWKLVWSDEFEGSEINMSNWS
                     FDRPDNGRYNGEIQSYTNKNAYIKDGKLIIEARKEDFTEPDGKTYHYTSSKLITQGKQ
                     KWTYGKFEIRAKMPEGKGIWPAIWMMPEDEFYYGRWPVCGEIDIMELLGDTLNKVYGT
                     MHFGLPHKQSQGIYTLPEGESFAEDFHVYSIEWEPGEIRWYIDGNLYHKESEWYSKAA
                     DKTNAYHYPAPFNQDFFLILNISVGGGWPGNPDETTVFPQQMVVDYVRVYQKEKYAIK
                     KE"
     gene            complement(26386..29367)
                     /locus_tag="PLG49_03475"
     CDS             complement(26386..29367)
                     /locus_tag="PLG49_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOQ16409.1"
                     /translation="MLELRARDVILNAEALLKREFGDILQDDYITDALFMDSSYKKAI
                     GITNLPVKMPIMTEIYTIKEITNYIEQLEKLEGSVLFLLDAYQKDLILFVKTKEKITE
                     IYIPQNIKSKILELISSIKDGVGIINEMGEHVFDLKAPSPGPHFYVNLLLGNRMGYNH
                     PLQTTPKSVVDGLGRGSFRAHSATQVLATRWDMLQEENGFPANRQFYIVEDGQQIFYS
                     ANPSDKNILSAKCTHSQNHTKICYTTCCGLKIERLIFILPQEDKIPMAVEVQQIKIEN
                     LTQKPRNLKIIYTGMFGSAVPHALMEDVLYSNIIMQFKTVMNEEGKLLAIAPDYYPEY
                     TKEDYRFHCTIIHHKGKIEYPAEFCTSYKEFVGNGSLEKPQGVIKLTNKLSRKGPGFF
                     AVGAKLNLDKEEVCVLDNFTGLVSRKLNPAFGDSIFKEEIKNLLDKYSQEGKVLEALN
                     KQKAFYDSYKRYFQVDTEDKNFNIYVNNNLPFQVLYQTFVSRSFGQTQKGYREIGFRE
                     IQDIFTSMHYFISMGAKDFVKALIKEWCNKVFEFGYTYHNFFWVGKEAGKWSDDGLWL
                     IQAVYRYINLTADINLLEEECEIAGTDPVKKRTIYDTIKAILKYSAEISVGKHKIPLL
                     DFADWNDCLKLDPDYMDGITKEKIYKEKLVKDGDTNIAFDNKYSESVMNGFLLKTALD
                     QMIDLTREKGDKAYTDKLIALSNQLYDNLQKYAWRENFFARVLFNRFTNGEFSYLGAK
                     GDGLSADPNIDGSYFLNSFSWSILSNTATEEQIATMLEIVESVLKTPHGLRLMSPTDL
                     GKVAKGTATGEYFPGDRENGGVFKHACMMATAAMFKAAKEVSDPVLAEKLSCLAYWTV
                     DLVLPYKTMEHPFETCGNPRFCTQYNNSETGENIGPILSGTSTWLTLTLMSALGIEQK
                     VNGIELNPILKNKQEKVSFTLNTGKAIYRFKISKPQGFFRLKEGHYKILLDSKEIGGN
                     IVPDLNDGKEHLVELLFYANK"
     gene            complement(29513..30793)
                     /locus_tag="PLG49_03480"
     CDS             complement(29513..30793)
                     /locus_tag="PLG49_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ16410.1"
                     /translation="MVNIILFICVLMTSCNTNRKKDEMLISETDENPIHLEIWTIWGN
                     NDILAGKALQNLVDDYMKRHPEVQITITTTTTETYKSKLPSALAVNEGPDIFFVWGYG
                     FAEPIVKSGKAVDLTPYVQEMKENALSNTFDALTYEDKIYGLPLFGWIMGLYCNTEMF
                     DRYNIKIPETYSELTEAVKEFRKHGLTPMALPGREPWAISFYYMALAEREAGLEKIQK
                     IAAGQEAFDDPSMISAAYKLKELKDLGAFSEDYYRLLSYEADSLFLEGFVPMHLNGSW
                     LSSTLDDEEYSKLKGKVKIIPFPVITEENGTDEGIGGYVDSFMINAESENKEEAIKLY
                     LDIMPKLSRELTQIGMGLPVWKDVRIDKEKTIYQFLEIYPEAGYHAAYDQILPQKVRE
                     VHLAYLQDLLEGRITPEEFVSIQAEKMKQYQNTD"
     gene            complement(30991..33027)
                     /locus_tag="PLG49_03485"
     CDS             complement(30991..33027)
                     /locus_tag="PLG49_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ16411.1"
                     /translation="MFYKNLKIGTKLNLSFAILIIVPLSIIGILSYSKMSSSLIEQNR
                     NNLIELMKQTSKSIDIAFMEIDRNMTALSRNEQIGSLVENYSKLDDNQKIRTKNQIQH
                     LMNDAINTRVEIADIYIITKNNDIFSSGVRVMDPKYDLISSYAVTKFKESERNSLWID
                     TYETDNDATGGISKRILTVFKNMYVSTSLKSVGTLMANVKEQYLYDLVKDIKMDYDGY
                     FFVIGQDKNIVMDPKNLNNNGMILKDRYLDKVFQEDTSWFIEEIDGEEMLVTFSKCKN
                     LDWVVVGLTPVKNITAPVTSMGFELLLIGIFCLIISFFAAVLITRDITKPMGLLIKQI
                     EKVKEGQLNIKYKVGRNDEIGILARCFRDMAERLKYLILEIKKMSGETYKLSQELSAT
                     SEENYATIEEFSQTINDIQEEIHSQVKGIETCSHVAFSLSDEIEDIIESFNHMNQKIY
                     EVKVSSEQGKESVGTLKDKAENLKEMIENVSAVVEGLINESKEISVITTTIKYIAEQI
                     DLLALNAAVEAARAGLYGKGFSVVAEEVKKLAEQTSSSVQYIDEKLNHMSMSIENVRK
                     VVISTEKFTQEHNIAVKDTSEKFELIDHSMDYVVEEIDGVKDLISNIEKSRKEIVVLM
                     ERLNNSSREHFASTQNLAAASSEEVELNKHLVEMAEELRALSAIVEEKISVFSL"
     gene            complement(33103..>33916)
                     /locus_tag="PLG49_03490"
     CDS             complement(33103..>33916)
                     /locus_tag="PLG49_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ16412.1"
                     /translation="AANILLFIIVIFTIFPIVWMFISSLMTQNNLAAGKITDITHWEN
                     YIEMWNNIDFFKYFKNSLIVCSITTIIALIIATFAGYSIARFKFPGSGLFGTAVLSTQ
                     MIPGIMFLLPLYMMFIKIQRSLGISIVNTYHGIIITYSAFFIPFSIWILRGFFASIPK
                     ELEEAAQIDGCSPFGAFMRIILPLSSTGIIATGIYIFLMAWDELLFAWVLATTPDVAT
                     IPVGIRLYVGNYQNRYDLLMAAGVVSTIPILIIFFTLQKKFISGITAGAVKG"
BASE COUNT        10638 a         6579 c         4523 g        12176 t
ORIGIN      
        1 taatttatta aaatattaat ttctttagaa caaaataaat atcaatccct ttttgcagta
       61 tcaaacagtt gttttaacat aataatcaat gtaatcttta agtttttcaa tatatctcat
      121 gccgatatgg ctaacgaccc cctgatttct cgtaatatat cctatattca tattaacatc
      181 tatatccaac gggatggcgc aataatcact gccatttaat tcttcacata taatacccga
      241 acataaggta taggcgttta agccaaccat taaattgagc atcgttgctc tgtcacaagc
      301 cttaatggtt ttcttataat cataagtact aagaatttcc tctgcaaaat aaaaagaatt
      361 attatccccc tgctcaaaag aaagacaagg ataatctgaa agctcttcca tgcttatttt
      421 gtcttttctg gctaaagggt gccctttcca taaatagaca tagcctttgc attgaattaa
      481 gtcatgaaat tccagttggt tttcttttaa taatttattg atcacctttt cattaaaatc
      541 attaatatat agaattccta tttcactttt tccatttctg acatctgaaa taacatctgc
      601 tgtccgtgtt tcgcggatag caaattcata ttcattcata tcaaattctt ttaccatttc
      661 tacaaaagcc tttacagcaa aggaatagtg ttgtgtagaa acgccaaact tcttttttat
      721 cttttttcct tcaatatatt tttcttcaag caattccatc tgtgccagga cctgacgggc
      781 atagcctaaa aaatcctctc cttcggctgt aaggatgacc cctttattag tacgggtaaa
      841 tatattgata ttaatttcag cttccaaatc tttaatcgca ctggaaagag tcggttgtga
      901 tataaaaagc cttttggccg cttcattcat cgaattggtt tctgcaacgg ttacgacata
      961 ttttatctgt tgtagagtca ttatatccct cctgctgaca gtttaaacta ctgccaatat
     1021 gatttattta taacttattt taataaatgc ataaaagaga ggaatatact ttccctctct
     1081 taaaataatc cgcttattca gctattgctt taaaagcctc ttctaaatct tcaatgatgt
     1141 catctatatg ttctgtacca atagaaagtc ttattgtatt tggtttaatg ccctggtcta
     1201 aaagttcttc tttagttaat tgtgaatggg tggttgatgc ggggtgaatg acaagggatt
     1261 ttacatccgc tacattggca agcaaggaga ataattcaag attatcaata aagtcttttg
     1321 ctttttgctc atcgcctttt atttcaaaag tgaaaatggt tcctcctccg tcaggcaaat
     1381 acttcttata aagttctgac tgctgtggat cctgagatac agaaggatga tttacccttt
     1441 ccacctgagg atgattcttt aaataatcta ccacttttaa agcattttct acatgtcttt
     1501 ctaccctaag tgacagggtt tcaagaccct gtaataataa gaatgcattg aatggtgcca
     1561 gggttgctcc tgtatctcta agcaggattg ctcttatttt tgtaacaaaa gctgcagctc
     1621 ctactgcttt tgtaaaacta attccatgat aacttggatt aggttctgtc agggagggga
     1681 acttgccact tccttcccaa tcaaacttac cactgtcaat aataacgcct ccaagggttg
     1741 ttccatgacc accaataaac ttcgttgctg aatggataac aatgtctgct ccatattcaa
     1801 tgggtcttat cagataaggg gtggcaaaag tattgtcaac aaccagagga attttatagt
     1861 tatgggcaat cgcagcaatc ttttcgatat caactacatc cgaatttgga tttcccaggg
     1921 tttcaacaaa tatagccttt gtattttcct ttattgcctc ttctatttct ttgtaattaa
     1981 agggatctac gaagcttgta gtaattccat aactcggaag ggtatgagct aataaattgt
     2041 aggttccgcc ataaatattt ttagccgata caatgtggtc tccagcctga gctaaattta
     2101 aaaatgtata ggttatagct gctgctcctg aagcaaccgc taacgcagca attcctcctt
     2161 caagggctgc aattcttttt tcaaatacat ccactgttgg attagtcagt cttgaatata
     2221 tgtttcctac atcctgaagg ctaaatcttg cttccgcatg ggcacagttt ttaaatacat
     2281 aagaagttgt ctggtaaatg gggactgctc tggcgtctgt agcaggatcg ggctgctcct
     2341 gtccaacatg taattgtaag gtttcaaatc taaatttcct atccgcatat tttttcttac
     2401 tcatcatcat acctccattt ttttatattt atctcaatta ttcttgcagt attcctatct
     2461 gttttatctg atatgtttat attaacattc aatcttgtta ttgtaaataa gtctttttat
     2521 ataattagtt ataaggtttt cctatagcaa aagaaatata aaagcagcct ttcgtttaac
     2581 aaaagctgcc tgttttactt tattactttt gacaaaataa tttttttaac cgtataatat
     2641 cccgcaaaag cagcaactgc tctcattgta tcaattgaat ggcacctttt catgttgaac
     2701 tcctctaaac tttttattca atatttttat caggaaatcg tcgagctttt taactgaata
     2761 agcagtcagt atggaaaata aaagaaccag ataaacatat aaaatatcac ttttaatcgg
     2821 aataaaatct acaaacaatc caataagtaa attatgaatt aaatacaatt ccaaggtcat
     2881 cgtacctaaa aattttagaa ctggtccgga acattttagc tttaagccca gggtaaatac
     2941 agtaaaaaca aaaaatacaa tcgcaggaac ttgaaatgcc aggcatatcc agcgttctgg
     3001 aattcctgaa cctcccttta actctgccca ataagaatat tttgaaagtg tatatagtga
     3061 tgccgcaaaa aataaaacaa ataaaagtat aaaaacaatc aaaataatac tatatttctt
     3121 cttaacaaaa tttacaatta tatcttcaaa tcttgcaacc agcatgccta caaataaaag
     3181 agggcaggta ttaaaccacc attcaccatg aagccagaga ttttcctgac tgtgcccaag
     3241 taacaggcta accgtaatat aacaaaaaat acccattagt gtaaaagctg ccttttcatt
     3301 ttttatatac ttaaagatta aataaaatgc tatataaaaa atgacaatcg caataatata
     3361 ccatgcatga gaattggcta agataagccc gctaagcctt aagagcaagc cagataaact
     3421 aaaccttgca ccaccaatat aataagcaaa taaataatgg tcgtaacata aaaagaaaca
     3481 agaactacaa gaagtctctt tttcaaaaaa gctgtttaag taattttcct ttgtacgcca
     3541 gcttctaatc agaccaaaac ctgatgaaaa aaagaaaaca cctacgagta atacgccaat
     3601 ttgactaaat gggaaaagaa gcctttgctc cgtaagcctt tgggaaagat gatgcagaat
     3661 aatcgtcatg gcgcaaaagc cctgaatggc ctttgtactt tccagggaaa gaaaatcttc
     3721 atgccagctt cccttttttg ttttaatcct ggacccaaac aaaagtataa aagcaagtat
     3781 tccataaaca aaagccataa aacccccaaa cttaatcttc ttgagatctt tattcagttt
     3841 tataacagta ttattgtaac gcggagccag taaaattaat ataattttaa ctaattttaa
     3901 ttaatttaat attaagattt gattttctac tattttgtgg tagctgtcta aatagagtta
     3961 atctatattg gcaggaattt ttatatctac tgtcatacca ggctttaata attctttacc
     4021 cttttcaata tcaattattg cctctagata aatatcacca tctttttcta ctgctctgtt
     4081 agatattgtt ctaattcggc cagtaatttc ctcgccattt tcgtacggag gaacaataat
     4141 ggcttccgcc ccggcagaaa ctaagctgat ggattcctca ggaatatctg caataaccat
     4201 catggtatcc tgatacatgg cttttaataa ggcctgctct ggtcctatcc tgcttccctc
     4261 tttacaataa atgtcatata taattaaatt gtcctctgga gcaacaatat aattgtcttg
     4321 tataaaagct ctatttaact tacttttcat ggcattaacc tgtaattttg ctgattcaat
     4381 tcttatactt aattcctctg tttcatacaa tttttgttta tccgtattag ctgcctgtct
     4441 tgaaatactt tctaattgtg ttttaaggcc atcaatttca atcctcctgt tgctttttac
     4501 tttttcaatg gccattaatg tatcctcttt gtctttaaga ttgcttttat agatttgttc
     4561 gctttgtttt aattcttttt cagtaattgc ccccatttca aataattcct gattggcttc
     4621 gtattcctgc tgagaaattg caatagcttt ttctataatt tctaaacgat ttttaagcgg
     4681 tatcaaatca ggatcttctc ctgttaaaag ataattttcc ttaatacgta actcattttg
     4741 aatgcgggag atatctggca taagtggatt gctattttca ctcagctttt ttagctgact
     4801 ttctaagatt tggacttcat tttcttttaa ttgtatttca agcaaataat cttttaaatc
     4861 cagtgcaatc aatttgtcgc cttttttcac cagatcgcca tcattaataa aaatggtttc
     4921 gattttagca ggaaaatcga tgacaaattc ctgacttcta tctaccttta catctccata
     4981 gacatttaga tattcttctt gagtcaacaa actttcattt tctgaatttg tatttacatt
     5041 ttgaccacaa gcagataacg aaaagattaa aaaaatcaac cataaaaact tatgttttct
     5101 tttcacactt tcacctcttt ctgcccagct attccctgtt ttttaaagct tctgtaaggg
     5161 caactttatc taaatttttt cttaagaaat ggcaagttat gaaataagcc aaagacataa
     5221 tgataaaact actgaaaaga gagagatttt taatccggac cataacatac tgctgcatat
     5281 ccgccaaaat accatcatag aggtttttag taatgatata gcccaaaggc cttcctccaa
     5341 aaaaagctaa aagtaaaaaa agataagttc ccctcatata catttgacta atttcatttt
     5401 gggagtagcc aaatattttt accatggaaa tatttgtttt agatttgtta atcaccatgt
     5461 tcatgagcaa atagataatc gtaatcaaaa tgcaaactgc agcaacaagc ataatgcctg
     5521 acatgacatt aatcatgcct aaaagattac caataccttt aatcatatca tttttaacgg
     5581 ttacagaatt gattttttct tcagctatat ctaaaggctc ataagtcatt aaagcattat
     5641 aactgtcttt acggatattg aatattttat taaagatata aatgttcgta tacaaatact
     5701 gaccataatc gtattctgaa gtatctttaa taacaacttc ataatatttg ttttcaatat
     5761 catcaaataa aagaagtctg tcacccagtt ccagattaaa ttttgttagt attgaagaac
     5821 taataagaac ttcattttct tttaaatctt ttaaaaactc cgatttaaaa aacttactat
     5881 caggctttat tccttgaatt gtaaaattct ttttttcctg attgataatg acttttattg
     5941 gcttaatagt tgtcagttcc ccttgcttac tatatttatg aagatccccc gggtcattaa
     6001 aagtataaat ataattaaaa ggcaccttgt ctataagctc ctctacaaat acaatacaag
     6061 aatctcttat ataagccgat gccattaaga cagtcacagc taataaaacg ccaattaata
     6121 atgcgagcat actccttcgt tctcttatca tccctttaag tctgaatctg gggaaaaaat
     6181 gaagactatt aaaggtacat attctttcta atttcgatac ctttttattc gaatactctc
     6241 cccgaagtaa ggaaagtggg gttttatcca gggttttaga cagataaaaa taagtaattg
     6301 ctacagcaag agttatcgga agaataattc caatgaatat caaatagaaa gaatctttta
     6361 cgataacatc cggcactgta tattctgtcc gttgaagaat aataattggt tcagatagta
     6421 agaccccgcc accgtatcct ataaatgtcc caattaaaac caataaaaca ggaagaatca
     6481 tataatgctt tatcatttcc ctcttattaa tcccctgggc atagagaatg ccaattgtct
     6541 gactttcttc ctggatggta ttttgaattg aaatggaaat aataaatgct acaataacca
     6601 ctagaagaag tcctatcacc agagccattt gttttggtcc ctctgaatca tcaaaaatag
     6661 tctctatcct gctgttctct tttttttcaa gaaaatctaa taatatggta tggtctctta
     6721 aatatttttt taacggttct atagaattcc ccattttttt atagctatag agtgtacttt
     6781 tgctttcatt tgtttttatc cgctcaaagc cttcttcatt taaataagct acaccaaaag
     6841 tttggggaga agttaaaaaa tcgcttgtat tcttaagcac ataaacataa tctggcgtaa
     6901 ttccataacc aactatttta aaatggctat cccttatgct aatgctgtct ccaatttgat
     6961 aattattttc tttagcaaat ttggggtcca ttacaatttc atctttactt gtgacaggct
     7021 taccctctac aatagccaac tgattaatct ctctgctggc agaaaaaatt ctaaggactt
     7081 tttcttcttt gcttttttcc gctttattaa agttaaaatc aatatacttg acctcttcaa
     7141 ttagcaggtt ataccgtttt tccatcgata atttttgttt ttttggttag ttttcccagt
     7201 atcttaaact gaccatcttc aacatgatgg ctgtcataaa agcggttagc tgaatatttc
     7261 ctcaaaaagc tctaaaactt ccctggatgt ctttgaatct aaagctcctg ttggttcatc
     7321 gcataaaaga agctttggat tcttaattaa agccctggct atcgccactc tttgctgctg
     7381 tcctcctgaa agctgggaag gatacttatg catatgatta ttaagaccta ctttttcaat
     7441 aagaaagtct atgtctaaag ggtctgtacc caaatgagca cagacctcta tattttccct
     7501 gactgttaaa ttaggaatca aattataaaa ttgaaaaaca aaaccgacat aatttctgcg
     7561 ataaagcgcc aattctttac tgtttaagct tataatctct ttgccatcaa tttgtacact
     7621 gccagatgta gcccgctcta agcctcctat aatattcatt aaagtcgact taccagaacc
     7681 agaaggacct aatattacag caatttctcc cttgctgact gaaaaagaaa cattatctaa
     7741 agcaagaaat ttactttccc cactgatata ttcttttgtc agggaatcaa ctgctaaaaa
     7801 caaatctttt ttcacccacc tttctattca agcactttat taacaaaagc tttaataaac
     7861 agctttaaaa tttcatgaaa atatttttcc ccaatgctgc tttgatgaat aaaaaggatt
     7921 tcgagaacat tcatgaaagc ttttataaat tcctccggga cagcgatttt tatgccaaaa
     7981 gctttaataa tttcctcaac atattgatga tttgcctctt catgctctgt aagtatttct
     8041 tcaggcacct ttcttaaaag atatggcatt tcgtccttta catgaaacat aattggtgct
     8101 tcctgaatat atctgatact ttcccaaata gccagttcta ctctgtcttt cagatccaga
     8161 tctgttcttt ctttcaggac tttcagagcc acagcataaa attcttcgtg aaaatcctct
     8221 atgacttcaa aaaataataa ttcttttgag ccataaaatt tataaaaagc gccttttgat
     8281 attccaacct tttgcaccag gtcatctacg gttgttttct taataccata ttgtatcatg
     8341 tactcccttg cagctttttt gagctgttct ctgattattt ttttctcttc ttctgtaaac
     8401 gcagaaccca ttttatcacc tactctatga ctaaaattat aattatagtc acagagtaac
     8461 accgtttaat tattttgtca ataattaatc atctgattta tttaaataga ttataaagat
     8521 ttatttaaat tgtctttaaa aaaaagtcct cctccggcat aaatattacc gttgaaggac
     8581 ttataacaaa agaatcttta ttatcacatg acataaaaag gatataaatt aaatcatgaa
     8641 aaattatatt ccgtaacaat tttttctctg cctgttactt gatcctgaaa atattcataa
     8701 taacaaatgc ctaaataaga gccagaaata ttatatacat tttcataacc taagttttgc
     8761 aataacatca cagcattata actcctttga gctgaacggc agtgaatata aacaggcctg
     8821 tctttaggga cttcatctat tctgtctctt aattcagata taggaatatt aactgcatta
     8881 atcaaatgac ccctttcata ctctgttctt tctcttacat ccacaataaa agcaccagtt
     8941 tctactaatt ctcttacctt gcttacaggc acctgtttaa aataaccatt taacaaattc
     9001 agtccaacta aggctgcctg gttaaccaca tctctggctg ttccatataa aggagcatag
     9061 gataattcta attcttttaa atcttccaaa gtacctccca ttaaaattaa tgtggcgatg
     9121 acgtcaatcc ttttatcgac attaccttta ccaatggcct gtgcccccag gattttaccc
     9181 gttggatatt cgtaaattaa tttaaaatga ataggataac tgtccggcat caaacctacc
     9241 ttatctttag gaatcacata gacaaaatca taagagatac ctgcctcttt agctgttttt
     9301 tcatttagtc ctgttaccgc tgctcctaaa tcaaaaaccc gtacaataga agttcctata
     9361 accccattat ttctatgggg aatattatag atatgatctg ctgctgctct ggcctgcctt
     9421 tgtgcgggac ctgcaagagg cagcctggtt ttcttatggg ttaagcggtg gtaaacttcg
     9481 atagcgtctc ctaccgcata gatgtcttta tcactggtta aataattgtg gtcaacctta
     9541 atagctcctg tttcgccaat ttcaaggccg gcttcttttg ccagagaaac ttctggtgta
     9601 actccaatag ccaatacaac ggccttagcc tttataattt tgccagactg taattctaca
     9661 aaagcttcgt taattctttt taccccatca tttaatatga gattgacccc tttatccagc
     9721 atttcttttt ggagaatttg taccatatcg taatcaaatg gacataaaat ttgaccctga
     9781 gcttcaatta aacttacatt ttttcctgct aaacgtaaat tttctgctac ttctacgcca
     9841 ataaagccgc caccaaccac cacaatatcc tgaatatgat gattatcgat gtaatcttta
     9901 agatttttta tgtcaataac attccttaca gtaaaaacat tagaaccatc aacaccttca
     9961 atactctttg gccgtacagg tctggctcct ggtgacaaaa ccagcttatc ataagcttct
    10021 tcatattctt caccagttaa taaatttttt atgacaatct ttttttctgc tctttttatt
    10081 ttaatgactt cgctattggt tctggcttca atattatatt gttttttaaa tttttgcgga
    10141 ttcataagaa ctaaagcttc actattgtct acaattccac ttaaataaaa aggcagtgaa
    10201 caatttgaaa aagatacatc tggccctctt tcaaagataa taatttctgc tgattcatct
    10261 aaccttctta cccttgtagc aacagaagct cctcctgcta cacccccaac aattaaaatt
    10321 cttttgctca taagttccac ctcctataat tagctttaac cttttataaa gcttatcatt
    10381 atcttcatat atcaggtata ccccctagga gtatgtttaa aataatcttt ttattaaata
    10441 taataacagg agtatttgca aaagtaaatc caagcaacta tattggattc tctatatcta
    10501 ttgaaatatg tcctgcaata attaaaatta tcaagaaata aacacatgac aaacttctta
    10561 ataccaaata ataataggta ccatgaatat ggtaccatgt cgttagtaat cgattttttt
    10621 actaatggaa atactaaatt catgattttt atagccttcc attctataaa tcacttcata
    10681 tttatcaact tcaaaagcta tctttcgatc tctttgcttt tcaatatatt cttgaattat
    10741 ttgatcaaat aaatcttttt ctataacttt gcagccataa acatcattta gatgatttaa
    10801 taatctgtaa aaatactcgc catcgtcttt attaatatcc acacacttga tagaaactcc
    10861 tcctaaatta gtatctgtac catacaaaat aaacttttct gaataactaa tagctgtatc
    10921 tggtaggtaa ttttgcttta taagaggtaa aatatcttga tagtaagggg catattcata
    10981 ttcttcacta tcatgatatc ctaagaagaa ctctattcca tctgacacct catcaatcct
    11041 tggttcaagt cccatttttt ctagtatctt taagctagct tgactacgct tatgagcttg
    11101 ttccatatat tcattatgaa attttccagc aaggcttatt cccctaactg ttaaaaagac
    11161 ggtaattact ataatagtca aaacatatcc aacagtagct agtcttaaat aacttgcttt
    11221 tatatccttt atacttgttt ttgtattcat aaactattca ccgccttgct ttcgataaaa
    11281 gaacaaagtt cttcgtaatc attgtatttg ctggaaataa aaaatttctt tcttttcttt
    11341 ttatcattat ctttatttaa atttataaca ttagctatta gccataagcc ttttttgttt
    11401 ttctttttaa ttttaaaatc ctcaaaatac catgtagcaa ctatacgaag attcatagga
    11461 ttatcttcgg caatttcttt aagtgtattt attgtaattt ttaaattgtt aatattgtgt
    11521 actaaagtta ctatcttacc taaaggaagc ttattaatac tcgaatgcct accaataaaa
    11581 tgcattggta catttgatgc taaagcatgt tgaaatgaat atagattatt attttcataa
    11641 tcaatagcat aaatatatga ccgtcttatt ctgtaatgac gtggataaaa tgaaataagg
    11701 aatataggaa taaaaattac cgcagttcca actaaaacat atgtaattat atcaacatct
    11761 ccaataatat taataaaaaa gaacagttct tctaatgctt ctaatactac ctgcgttgaa
    11821 taatctagaa atacccaaga tataaatgaa ataaaaaata tacttaatcc aataggaata
    11881 aatataagaa aagtaagccc tataataata tggataaatg aagctttaaa tatagaactt
    11941 tcatattcaa atgttttcat aaaatacccc caaatgctaa ttatttaatg tttcacacat
    12001 cattattata ccataaatta cataaaatca aattattata aatattaata gtaatttctt
    12061 tatttatctt tttttactcc gccatttcca gagcaatttt catcatatgg gtaaaagcat
    12121 tttgcctttc ataggaactg gtctcttcct tagttactaa attatctgag actgtcagca
    12181 gacaagctgc tttcttccca agaatttttg cattatggaa taaggcaaag gactccattt
    12241 caacggcctc acagccgtat tttttattaa tttctttata tgcttcagga ttttccctgt
    12301 agaaaacatc cgaagaatga attcttactt cgcggattgg aatatttaat tcctcagcat
    12361 attttttaag cctttcattt aactctgcac ttccttcaat aatatctttt tcataacctc
    12421 cctgtactct ggcataagtt gactctgtcc aggcatcttt gaccagaata acatcatata
    12481 attttagatc tgctgtatag gctccacagg aaccaatcct gatgatattt tcgacatcat
    12541 aaaatttaaa aagctcataa gaataaatcc ccatactggg catacccata ccactcgcca
    12601 taacagagat ttttcttcct ttataatttc ctgtaaagcc taaaacattt cttacattat
    12661 taaattgttt tacatccgtt aaaaaattct ctgcaataaa tctggcacga agaggatctc
    12721 ccggcattaa tacagttttt gcaatttcat ttttgtcttt tgcttcaata tgtggtgttg
    12781 gtatcatctg atttcctccc ttgtaataat ctttcttatt ttttactcct atccatagta
    12841 tatcaaattc tttttattcc gtatagcttt ataaaaataa aagtattctt attatatgtc
    12901 tgttaatatc ttatatttct acaaaaaaat atatagataa tttttcataa agcagttgac
    12961 aaatcttttt ttaaatgtat aattaatatt aagtaataat aattatcaat tacatatact
    13021 ttttattaca agttaataat aattataaaa tatataggag gtatgtatta tgtcattaat
    13081 aggaaaagaa gtaaaacctt ttaaagctca agcttttcac aatggagaat ttatagaagt
    13141 taccgaacag gattttaaag gaaaatggag tgtagtgtgc ttctatccgg cagacttcac
    13201 ctttgtttgt ccaacagaac tggaagattt acaagataac tatgaaactt ttaaatcctt
    13261 aggtgttgaa gtatattctg tttcaaccga tacccattat actcacaaag catggcatga
    13321 cagctcagaa accatcaaaa aaattacttt cccaatgatt ggagacccct ctcacatcct
    13381 tgcaagaaac tttgatgtat taattgaaga agaaggtatt gcagaccgcg gtacctttat
    13441 catcgacccg gatggcgtaa ttcaggctat tgaaatcaat gccggcggca tcggtcgtga
    13501 tgcaaaaact ttaatcagaa agattaaggc agcgcaatat gtaagaaata acccaggaga
    13561 agtttgtccg gctaaatggg aagaaggggc aaaaaccctt aaaccaagcc ttgaccttgt
    13621 aggaaaaatt taagacatac cgaggtgaaa gttatgattc tggagccaga tataagaaat
    13681 caacttgatc aatatcttca actgatggaa aatgacattg tcattaaagt tagtgctggc
    13741 tctgatacaa actctcaaga catgctttct ttaatcaatg aaatagcttc cttatcccct
    13801 aaaataaaag ttgaaaaagc tgtcctgccc aaaaccccga gttttagtat taatagaatg
    13861 aatgaggata ccggcattat atttgccggt attcctttag gacacgaatt ttcttctttg
    13921 gtccttgcac ttttgcaagt aagcggaaga ccaccaaaaa ttgaagcaaa agtcattgac
    13981 agtattaaaa atttaaaagg ggaataccat tttgaaagct atgtcagtct aagctgtcat
    14041 aattgtcctg aggttgtaca ggcccttaac ttaatgagtt tattaaaccc aaatatcacc
    14101 catacaatga tagatggatc tatttttaag gaagaagccg aaagtaaaga tattatggcc
    14161 gttcctgcag tttttctgaa tggcgaattt tttagcagtg gacgtatgac ccttgaagag
    14221 attctcgcta aaatcggaac aagtcctgat gcttctgaat taaagaataa agaagtcttc
    14281 gatgtacttg ttgtcggagg cggtccggct ggtgccagtg cagccatcta ttccgcccgt
    14341 aaaggcctcc gcactggtat tgttgcaaaa cgtttcggcg gccaggtcct ggataccatg
    14401 gccattgaaa actttatcag tgtaaaatat acagaaggtc caaaactggc tgcagacctt
    14461 gaagaacatg tgaaaaatta tgatattgat atcatgaagc cttatcaggt aaaatccctt
    14521 cgaaagaata aatttgttga aatagaatta gaaaatggtg cccttttaaa aagtaaaacc
    14581 gttattattg caacgggagc ccgtccaaga acccttggtg ttcccggaga agcagaattt
    14641 agaaacaaag gggtcgctta ttgcccccac tgcgatggcc ctttgtttga aggaaaaaat
    14701 gttgctgtta ttggcggcgg taacgctggt gttgaagcag ccatagatct tgctggtatt
    14761 gtcaatcatg ttaccctgct cgaattttta ccggaactta aagctgatgc cgttttgcag
    14821 gaacatcttt acaaactccc taatgtaact gtgcttaaaa atgttcagac acaagaaatc
    14881 actggcactg aaaaagtcaa tggtatcact tatttggaaa gagatactgg tgccgttaaa
    14941 catatacccc tcgatggcgt ctttgtccag attggtctta tccccaatac agactggctg
    15001 gaaggaacac tgactctcaa tcatttaaaa gaaattgtag ttgataaata cggcgctacc
    15061 aatatccccg gcgtatttgc cgcaggagat tgtaccgaca gtccttataa gcaaattgtg
    15121 atttctatgg gttcaggagc tactgctgca ttgagtgcct tcgactacct aatcagaaat
    15181 taaatatcaa aaggaccttg caattattgc aggtcctttt taattttcct cattagctgg
    15241 cacttctatt cttacaataa tattctcatt gccacaagtg atatagccta tctttaattt
    15301 ttgtccattt cggttaatga tacccccatg acttcctgca cgatgatatc ctaaacctac
    15361 actataatgc tcataatcaa atttaacatg attgatttca aaactttcgt gatgaccccg
    15421 gccttcataa ggcattggta cataattttc gacgtagcct tcaactattt tataattgcc
    15481 ttccttataa ggaataacaa tggtcttata ttcctgaata aaattattca catacaataa
    15541 acaaaacatg aaaacaaaaa ctgcacagat cctggaaaat attttaatga tacgaatact
    15601 aaattctgta ttatattgtt ctttattatt tattatattg caatatacat aaatcttagg
    15661 aaagaaaaga agaaatataa acattataaa aggaaaaaac caggcgaaat caatgtcctg
    15721 tgtcacttca tataccaatt tataatccat tgaaatcagc ccccatcagc atgaagatta
    15781 attttatagt tgtataaacg ctttatcaaa ctaatttata atgaattttc tgaactaatc
    15841 tagcttatta gaaaattcaa gtcaaggtaa tagaaagctt gatttttgac attcctatat
    15901 ccgttttatt aaatttgaac tgtttctcca attccattag gagactatat tctaaatatt
    15961 tattctaata attttcttta aatgtctaaa ctcagtctaa caagaaacac tcttcaaact
    16021 cgttttaatt ttttattgtt ttaacaaatc taccataatt accatattca aatttccctt
    16081 ctgcccctat ttcaaaattg gcctttgcta tccctaaatc aaccagatta aaatctgatg
    16141 tatccttaac ataagcatat acttctcctt ttttataaac aaatcttaca ggatgacggt
    16201 ttatggcaga tggagctagc tgagctgctt ttacgccttt cataaaccaa gacggaactt
    16261 ccatatctgc ctcataaagt tcttctaatt tgatactttt ccgatgagtc atgtgatata
    16321 tggctttttc ccttaggcca ggcttttcat tgacaggacc cattgtaata acacaaataa
    16381 gggattcatc tgctccaatt tgacaagggc agttcttttt gtcataactg gctcctacaa
    16441 aacaagttcc cagtcccaaa gctgttgctt ccaggataag taactcccca aaatacccag
    16501 ccttttcttt tatatgcgga tctgctgttt ttcccaccag agcaattaaa gttcttacac
    16561 cataaaacat cccgtagctt ttcctcaggc cattaaaagc ctggctgcca tcttcaatca
    16621 attggataga aagtcctgaa agcttattat aatgcctgat tgcctcccgg attctttcta
    16681 atttgtccgc ttcaattttc tgattcagat aggcccttct ggaaaccctt ttttcaatgg
    16741 ctttaagata gtttatccga ttccctttat catataataa atccattcta tctgcccttt
    16801 ccgtaatttt ttctatacta atggactgca ttgataagtg cgttttctat ggctttttta
    16861 atgacaacgg atgaatttgt tgcaccagat actgtatcta cctctaagct ttgagctgct
    16921 ttaatatctt caacaatttt ttctgctttt tgccctcttc catttttatg ttccagcaat
    16981 ttaatgtcgg taacctgacc attttttacg gtgacttcaa cttttgcata tataaagtca
    17041 acatcataat cccctacata aaccccatca ggaatttccc tgaaatcaag agggctgatc
    17101 ctggtactgg ctattttttc tttgtagtta ttaagatcat ttaaataatt taataaaata
    17161 agaaagccca caaatatcac aacaacaatt aatagtatta ttttcttttt cttcataaga
    17221 tcctggcaac gtatttgctg caaatacatg cctaactcct ttctaaacaa aataatttat
    17281 tcttttttta ttgaatgata aagcaattca aaactgtatt gtaagaagtc ctctgaggaa
    17341 acccccatgc ctttttgaag atatttattc ttttcttttg acatccggat aatgccataa
    17401 atacttgccc aaaagataaa agaagccagt ggcaattgga tatctcttct aagcaaatct
    17461 tctttgacgc cctcctggaa aatttttaat atttcctgat tgatttcttc tccaacctca
    17521 tatatttcat aataaacctt tggtgtttct tctaattcta aatctacatt gatttctgaa
    17581 aaaacacatt caaaataaaa aggatgctcc ttttgaaagt ctactaaagc ataacataag
    17641 gcaaaatact gggatttaaa atccttatgt tccataaggc agttccttac ataatcaaga
    17701 aaggctttca ttcccaataa ggtgattgcg ccaatgattt catctttatt tttaaagtaa
    17761 acatatagag ttgccttact atatcctgct tctctggcaa tatcgtccat tgaagtattc
    17821 tctatcccat ttttcaaaaa taacttttcc gatgcccgta tgatattgtc cctgtgaaac
    17881 tttgccagga cttctttttt actttctttc ataattctcc ttcttctttt catgttttta
    17941 cagtcatctt atctttttat tctcttgttt cttaattttc aaaaattaaa ctaagagtat
    18001 attatttata cttatagttt agtataaact tttatccagc aaatgtcaat ctataatgat
    18061 tttattcaag attttttaac aattaatagt tgaataattc attatgaacc tctggaacta
    18121 attaaaagtc cctgattcct ttttataaga aaaattaaag gactgtcggc taacggttat
    18181 ttaaccatta gtacacagcc ctttgctttt ccattagaca tctttaagcc agttcaaccc
    18241 ttatttcatg gatcttatcc ttatccaaag cctcaataac ttctcttttg attaaatagt
    18301 ctgattttgg cttgatcagc ttttgttcac cctgattaat ccatactctt ttaatttgat
    18361 atttaccagc ttctaatcca tttgtattga catatgtaat ttttaaaatt ctatctgcaa
    18421 attttgtcac aactgctgct tcgtttaaat gattaaattg ctcccttctt agttgtggct
    18481 taagtcttaa attgcccaaa tctcctgtta caccataaat ttgtgttaag accgtcagca
    18541 ataaccagct tgcagaacct gtaagataat gatacatacc ccggcccttt tcattgatat
    18601 attcaggaat accaggataa atcctgcttt cttcaaagtt tttgcactga ttatagataa
    18661 gttgaatgac ttcataagct tctttggaaa agcctctttt gtacaaagca ttagaataca
    18721 tcactgccat atggctaaac atcgctccgt tttctttatg tccgaaagca aagccaaaag
    18781 ctcttcctaa atcttttttg agttctttaa agtttgtatt taatctgtag ccaccaatct
    18841 taggattctt caggtacctg tcacaagctt taacaacttc tttgacctgt tccccggttg
    18901 cgatatctcc cataagggta aaaacctgtc ctgttaaagt catcctcact ccttcaggaa
    18961 aatctccttc aacccttctt ccgtggttat cgtaataacc attaaaccac tgataacctt
    19021 cttcatttgt tataaattct ttatttctga tatgctcttt gatccaattg gctttttctt
    19081 tcaaatccct cgccaaagct gtcaaatcca cttttacttt ttctccggaa atcgtatgct
    19141 tacaagcatc aaaatacctt tttaacagtt ccttcttttc ttcaacagaa tcataattta
    19201 caggctttat catcgtatcc agtaagacaa gcatttcctg agccagttct attgtattaa
    19261 tttttttctt ttcctttagt attaaaatca tgtcactgat ttcagccaga ttgtttccat
    19321 ataaggcagt aaaggcaacg ctttcaccat tttgggctgc catgtcaaag gcatcattcc
    19381 agtcggctcc ctctaaacgg atattattgt gttcccccac attataaaaa ggtgttaaat
    19441 gttggagcat aatatgttct aaaatcgttc ctttatatat ttctccttta tgcgttttta
    19501 aaaggttgcc ctgggacttg tcccataatt catcgatttc cgtagatctt gccgcctggg
    19561 aatctttaaa ataagtttgt tcttctaata aaaaatccaa gtccccgctg taatcgatat
    19621 aaagctttgt tgtaatccag ggccaggcac catgatccat ccatattctg cagatgttgt
    19681 ttctgtcagc aatgaattct ccgggctttg ttccaataat ggtagcattg ctgccatcga
    19741 tacggactcc tccatagtta ttgattaaca aatgacggac ttcttcaggt tccatcatta
    19801 aaagtgccag gcaatcctgc caaaggtctc tccagcccct tccccctctt ccataatcat
    19861 ggtggggtaa aaaagaacag ccatatattc ttcgtaaaat gggttgcagg gtcacccatc
    19921 ttagccacaa atcaaattcc ctgtcagcag aataaaactg caacacgtca agtttctgct
    19981 tccaataatc catattttga tttaaataat aataaaagtt ttctttactg cagtatttac
    20041 ggactatctc ttctcccctg cctctgtctt ccgcaatata aagagcaagg atatagcttt
    20101 tttcctcccc tggttcaagg atttcctctt taaaacgaag ggctccaatg gcttcataac
    20161 cttctatgat attgccttga taaacaaaag agtccttgtt ctttacaata acctcaggcc
    20221 actctaaaga acccccttca ccaataaaat cctctactaa aggaaaactg cccaaaggct
    20281 tattcccttc ttcatcccgt gcaaccaccc cataagtcac tttattttct ttatgccctc
    20341 tttcatcaaa ggacaaagca ggctgcactt ctatgccact ttctatggta tatatcctgt
    20401 gaagcaatga agtcacatga cgatggtctc ttaaattatc tgccgatctg ccaaaaacag
    20461 ggattgaact ggtaggcgta atgagcattt tatcctttcc agtattctta atggttactg
    20521 ccattagttc aactttatca tttgttgcag gcacaaagtt tataatttca gactgaatcc
    20581 cgattttttt attttcgcgg atgactttat gccacaaaaa acccgcctct aatttaactt
    20641 cttctgcttc ttcatcaaaa gtatccatga tttgaccagg agaatgccct gctgccgacc
    20701 agggtcctac cccttcgata taaagccaaa aatttctccc tgacctgcta ttatgcaaat
    20761 cctctgaaga aaccggaagc atgaaaaatt cattttgtcc tgctttaata tctccattta
    20821 ataagggagt aatcgaagac atcatacctg catcattaac caatggaaaa tacaaataac
    20881 tgtttttatg aggggctaat aaagaaaagc tgccgttatc atctataaag tgccacgccg
    20941 tttttttgct tgtatccatc cctatcttcc tcctaaaaaa tactatataa ttcaagttta
    21001 actgtaattc ctgttttatc gcagtttatg attttgatgt catagttttt atccgcttga
    21061 cttgattttc caatatctaa tatttcttct tttagtaatt cttcttcttt cttaccaagc
    21121 aaatttcttt gtaaatcttt taggttattt ctaattgtta caacaacgga attacttttc
    21181 ggttctattg ctatttttat atggctgact aaatcttttt tttcaatata atagtgaatc
    21241 atcctattaa ctattgattg aattaaaaac ctgggacaca ttactgttaa cataatatgg
    21301 ctgctatcaa tttcaaaagt taagttttca ttataacgga ctgcctgaat tgttaaatac
    21361 tcatttgtaa aatctatctc gtctttaagc agaaccgttg cttgattatc attgatcaca
    21421 aagcggaaca tgtttgctaa agcaagcgtt actttacata tttcacttgc accaatggat
    21481 aatgctaaac agtttattgt ttcaagagag ttaaacaaaa aatgatagtc cattatttta
    21541 tcatcaaaat aaaagtctgt ttttttgatt tgattcatat tcttttttcc catttaacct
    21601 atcctgtcat ttataatatt tttgttgaat tgatataaat ttaatgcttt tcgcataagg
    21661 tgtctgatta taaaaaaata tagaattcta actatttttt atcgggatat ataaatacac
    21721 ttttgtatat ttaccttcta tgctttcaat tttaattatc tcgtttatat cttctacttt
    21781 ataataaata cttagtcttt gaataatgct tcctaatcca aatccagatg ttttattcac
    21841 ttcctccgca cctagtattt taaaaattat tttttctggt attcccactc catcatcttc
    21901 taccgcaata aagattaagt ttttatcttc tattttttta atgatacatt taatatttaa
    21961 agccttttca aaatccctga taccatgatt aatagagttt tctaccaaag gctgaagagt
    22021 taatttagga attgtacaat ctaacacagc atcatcaatt tcataactaa cttcaaataa
    22081 tttgttcatc cggatattca taatatccac ataatttttt agtaattcca attcatccct
    22141 aatagtaatt atatctttac cactacttaa acaaagtcta taatatgccg ccaaggtccg
    22201 taaaatataa aatgcttctt cttttttccc aatacttata taggcactga ctgcattaag
    22261 ggtattatat agaaaatgag gtttgatttg gcttaccaat aaatcaaatt ccagtttcct
    22321 ttgtatttca tgctctttat ttaatttttc taaaagtaaa ttaatctgat ccatcatcat
    22381 atttaaggct gcgctgagtt gattaatctc atcattacta ttagttattt taaatcgtgt
    22441 tgttaaatca ccgtctctaa tcttattgat agaacttgat atggattcta acggttttaa
    22501 aataactgaa ttaatgatcc tgcttcctat aatggataga atagcataaa gtaaaattaa
    22561 aaatataaaa attaattcta tagattgaaa attattatca tatacaaatg cacaggataa
    22621 atcccagtca atagattcta aaggaacttt gatgaaataa tgtgtttgct tttcaatttg
    22681 ttttttataa tattcatcca ctgtataagg aataagcgac aaagtttttt tcacttcttc
    22741 tgctctgcca tcataaggaa atataataga ctgcttttta ttatcttcta tcatatacga
    22801 atacatatca ttactggaaa atttcttata tatatcttcc agaacattat ttctaaccgt
    22861 tataacaaca tatccaatcg tctgatagtc ctcaatgcta ttaatagccc tggatactgt
    22921 catataatta ttatcgtctt gaataaacat atttttttgg atattccact tagatcttcc
    22981 tttcagggat accaattctt cagtatcaag agaagctaaa aaatcatcgg ggaaacgagc
    23041 ttctttttga tcccggtcta cataatatat ataattattt ttatcaataa tataaattga
    23101 agcaatatca acatctctat taatagtatt atttaagtat tcattgattt ctcgttctga
    23161 aatatagtag tactcttttc ttccttcttt taaagctttt tgaacactgg gattaagtgc
    23221 aatatctgcg gatatcatat ctactctgta aatgacactt tcaatggttg tttttattaa
    23281 aaacacctgc tgctggtcgg ttttaaacat ttttctttct acttgccttg catttattct
    23341 aatatacata acaaaactta cagacaccaa tacaagcatc attgtcatgt acccaatatt
    23401 taattttgac cggattccca ttttatgaaa aaaattatat ttcaacatac tccccctctt
    23461 atctctttta tctacagttc tttttatatt ctgccggggt gaagcctgta tactttttaa
    23521 ataatttact gaagtaaccc ttttccttat aacctaataa atcacttatt tcatatatct
    23581 tatagttgtc ttcttttaat aatacttttg ccctgtccat ttttgcctta gtaagataat
    23641 ctaaaaagtt ttctcctgtt tcttgcttaa ataatgagca aaaataattt ttacttatat
    23701 gaagatattc agaaattgaa gctaaagtaa ttttttcatc aatgtgttcc aaaacatatt
    23761 cacaagccat attgactatc gtactattac tgtttaatcc atatttttgt ataacatgga
    23821 ttaactctgt cacattatta ataaaatgta cttttatttc ttcaatatcc ttatatttta
    23881 aaaagcctaa atcttttact tccgttaaat tataaatctc tttaatataa cgatatttat
    23941 cctgaacagc ctgataaata ttggttgcca attgagtaaa acaaagacta attttatata
    24001 aatcataatt attatttttt attatctgat ctaacagttc ttctaaaaat ttagcagatt
    24061 tactgtctcc aaaaagaata taagagataa gaatcttttc ctcttctaaa ggataaataa
    24121 atttgttttg ttcatgggaa tctaactgca atatatgaat aattttattt ccacctaaaa
    24181 catacttatg tttaagactt tctttcgcat taaaataact taagtaagtt aattcgactt
    24241 tttcatatac tttacctatt cctattgtaa ttctaaggct caagtcatta ttgattttat
    24301 gtaaaacttt atccaaaact ttggatatga ttgcatctag ctgttcttta tccgaaatgt
    24361 cttttgcctg aatgattata ttaatacttc ctaatccacc catactaata atactggtta
    24421 tatgattttc cttttccaat tcttgcttta taatggtttg aaccttatca gcaatttctt
    24481 catacatccc atttaaaagc caatcttctc cctgctcctc aaaatcatca agttctatga
    24541 ttgccacctg tacaaaatga ttggttaatg gaatatcata ttcctctatt aatgtataaa
    24601 gcttatccag attttttccg tgtataatat cactaatcac cttttctctt aaaaatgacc
    24661 tggtcaattc taatttttcg tttagatatt gtgtatgctc ttctttcttt ttttcatcgg
    24721 ttaacctttt atgtactttt tttaatactt cctcaatatt gactggatcc ataggtttta
    24781 aaagatagtc cattgcacca agggataagc ctttttgtgc ataggcaaat tcagcataac
    24841 cactcaacaa gataactttc acaggattat ctttttctct taaggctttt aacagctcaa
    24901 tgccatccat ttggggcatt ttaatatctg taatgatcaa atcgattgct tcattttcaa
    24961 gtttgtttaa ggcttctatt ccattttttg cttctgcaac aatttgaaag cctaagcttt
    25021 cccaatcaac gacatgtctt aatcctgttc ttataattgg ctcgtcgtca atgaccataa
    25081 ccttaaacat atatatcctc ccaaaatata agaaataata atgattattc atataaatca
    25141 gtatgaataa aagaaattga ttacttataa cttcattgta cttaatcata aattgtttaa
    25201 ataaatagaa taaaagttta aagttaaaaa ttttgtatat atatttaaaa gagggtctta
    25261 attttgtacc cttatacaaa attaagtatc cctctttatt atactgttat ttgtatatca
    25321 attaaaatat ttgctttaat ttttataata caaaagaatt tatttcatct aagtctttta
    25381 ttcttttttg attgcatatt tctccttttg atataccctt acataatcta cgaccatttg
    25441 ttgtggaaaa actgtcgttt catccggatt accaggccat ccgcctccga cagatatatt
    25501 taaaatcaaa aagaaatcct ggttaaaagg agcagggtaa tgataagcat ttgttttatc
    25561 tgccgcttta gaataccatt cactttcttt gtgatataaa ttgccatcaa tataccatcg
    25621 gatttcaccg ggctcccatt caatactata aacgtggaaa tcttctgcaa aactttctcc
    25681 ttctggcaag gtataaattc cctgtgattg tttatgtggc aagccaaaat gcattgttcc
    25741 atagaccttg ttaagcgtat cccctagtaa ttccattatg tctatttcgc cacatactgg
    25801 ccatcttcca taataaaact catcctcagg catcatccaa atcgccggcc atatgccttt
    25861 tccttcaggc atttttgctc tgatttcaaa tttcccataa gtccattttt gctttccttg
    25921 agttattaat ttggatgaag tgtaatgata ggtttttcca tccggttcgg taaaatcttc
    25981 ttttcttgcc tcaataataa gttttccgtc ttttatatat gcatttttat ttgtatagga
    26041 ttgtatttca ccattatatc ggccattatc cggacgatca aaactccaat tgctcatatt
    26101 gatttccgaa ccttcaaatt catcgctcca aaccagtttc cactcttctt cttttactct
    26161 ggctgtttca caaccattaa tattcgcttg atttttaatt tctttaaaac tcatttttta
    26221 acctccggtt aattgtttaa ttgttcttat ctaaaaaaca atttaaatcc ccatcagcct
    26281 gattctttat ttatcatagc ccaaatatca acaacccact ctcatgttat ataaaaattc
    26341 aaaaagaata aatatgacaa aattatcttt tatggtaaaa tattatcatt tattcgcata
    26401 aaataatagt tcaacaagat gttctttccc gtcatttaaa tccggaacta tattcccccc
    26461 tatttcttta gaatccaata atattttata atgaccctcc tttaatctga agaagccttg
    26521 aggtttagaa atcttaaacc tatatattgc cttacctgta tttaaagtaa agcttacttt
    26581 ctcctgcttg ttttttagta tgggattaag ctctattcca ttcacttttt gctctattcc
    26641 caatgcagac ataagggtca gagttagcca ggtagatgta ccactaagaa ttggtcctat
    26701 attttcacct gtttcactat tgttatactg cgtgcaaaat cttggattac cgcaagtttc
    26761 aaaaggatgc tccatggttt tataaggtaa aactaaatcg acagtccagt atgccagaca
    26821 actcagtttt tctgcaagca caggatcact aacttctttc gcagctttaa acattgcagc
    26881 agttgccatc atacaggcat gtttaaatac acctccattt tctctgtctc ccgggaaata
    26941 ctcccctgta gctgtaccct tagccacctt tcctaaatca gtcggactca ttaatcttaa
    27001 tccatgaggc gttttaagta ctgattcaac aatttctaac atcgttgcta tttgttcttc
    27061 cgttgccgta ttacttaata tagaccagct aaatgaattt aaaaaataac ttccatcaat
    27121 attgggatct gctgataaac catctccttt tgcccccaaa tatgaaaact caccatttgt
    27181 aaatcggtta aacaataccc tggcaaagaa attttctctc catgcatatt tctgtaaatt
    27241 atcatataat tgattggaca aggcaattaa tttgtctgta tacgctttat ctcctttttc
    27301 ccttgttaaa tcaatcattt gatcaagtgc cgtttttaat agaaaaccat tcattacact
    27361 ttcagaatac ttattatcaa aagcgatgtt tgtatctcca tccttaacca gtttttcttt
    27421 ataaattttt tcttttgtaa ttccgtccat gtaatcagga tctagtttta aacaatcatt
    27481 ccaatctgca aaatccaata aaggtatttt atgtttaccc actgaaattt cagcagagta
    27541 ttttaaaatg gcttttattg tatcgtaaat tgttctcttt ttaacagggt ctgtgccagc
    27601 tatctcacat tcttcttcta ataaattaat atctgctgtc aaattaatat agcgatatac
    27661 tgcttgtatt agccacaatc catcatcaga ccacttgcct gcttctttcc caacccaaaa
    27721 gaaattgtga taagtatagc caaattcaaa aactttattg caccattcct taattaaagc
    27781 tttaacaaaa tcttttgcac ccatgctgat aaagtaatgc atagaagtga aaatgtcctg
    27841 gatttctcga aaaccaattt ccctgtaacc tttttgtgtt tgtccaaaag aacgggatac
    27901 aaaagtctga tataaaacct gaaaaggtaa attattgttt acataaatat taaaattttt
    27961 atcttctgta tctacttgaa aataacgttt ataagaatca taaaacgctt tttgcttatt
    28021 taatgcttct aaaactttgc cttcctgact gtatttatcc aataaatttt ttatttcttc
    28081 cttgaaaata ctgtcaccaa aagcaggatt aagctttctt gatactaacc cagtaaaatt
    28141 atccagtaca caaacctctt ctttatccag gttaagctta gcccctacag caaaaaaacc
    28201 tggtcctttc ctgctcagtt tattcgttaa cttaatcaca ccctggggct tttctaaaga
    28261 accattgcct acaaactcct tgtaacttgt gcaaaattca gctggatatt caatctttcc
    28321 cttatgatga atgatggtgc aatgaaaacg ataatcttct tttgtatatt ccgggtaata
    28381 atccggtgca attgcaagta gtttaccttc ctcattcatg acggttttaa actgcataat
    28441 gatattgcta taaagaacat cttccatcaa tgcgtgcgga acagctgagc caaacattcc
    28501 tgtataaata atttttaaat ttctgggttt ttgtgttaag ttttcgattt ttatctgttg
    28561 gacttccact gccataggta tcttatcttc ttgaggaagt ataaaaatca gcctttcaat
    28621 ctttagacca cagcaggtcg tataacatat ttttgtatga ttttgagaat gcgtacattt
    28681 tgcagataaa atgtttttat cgcttggatt tgctgaataa aaaatctgct gtccatcttc
    28741 aacaatataa aattgtctgt tagcaggaaa tccgttttct tcctgaagca tgtcccatcg
    28801 ggttgctaaa acttgtgtag cagaatgtgc cctgaaactt cccctgccta aaccatctac
    28861 tacactttta ggagtggttt gaagcggatg attatagccc attctgtttc ccagcaataa
    28921 attcacataa aaatgtggtc ccggtgaagg tgcttttaaa tcaaaaacat gctctcccat
    28981 ctcattaata atgccaacac catctttaat actagaaata agttctaata tttttgactt
    29041 gatattttgc gggatataaa tctccgtaat cttttccttt gttttaacaa ataatattaa
    29101 atccttctga taggcatcca gcaaaaaaag aacggagcct tccagttttt ctaattgttc
    29161 tatataattc gttatttctt taattgtgta aatttctgtc atgattggca ttttaactgg
    29221 cagatttgtt attcctattg cctttttata actgctatcc ataaaaaggg catctgttat
    29281 ataatcatct tgtaaaatat ctccaaattc tcgtttaagg agggcttctg cattgagtat
    29341 gacatctctt gcccttagtt ctaacatatc atccactcct tctaaaaata tttagtatta
    29401 tttcttacta ttattttaaa acatttttta ctctgtccat agaccatata attatgattt
    29461 tttagtataa aattataata ataagtcatc ccattactgg atggctttat ccttaatccg
    29521 tattttgata ttgtttcatt ttttctgcct gaatactaac aaattcttct ggtgtaattc
    29581 ttccttccaa taaatcttgc aaataagcca ggtgtacttc tctgactttt tgaggcaaaa
    29641 tttgatcata tgcagcatga tatcccgcct caggatatat ttctaaaaat tgataaattg
    29701 tcttttcttt atcaattctt acatccttcc agactggcag ccccatacct atttgagtta
    29761 attctctgct taacttgggc ataatatcca gatataattt tattgcctct tctttatttt
    29821 cactttcagc atttatcatg aaggagtcaa catatccccc tattccttcg tctgttccat
    29881 tttcttctgt gataactgga aaaggaatga tcttcacttt accttttaat ttggaatatt
    29941 cctcatcgtc taaagtactt gacagccaac taccatttaa atgcattgga acaaatcctt
    30001 ccaaaaaaag tgaatctgct tcataactta ataaacgata ataatcctca gaaaaagctc
    30061 ctaaatcttt taattctttt aatttatacg ctgcactgat catagaagga tcatcaaaag
    30121 cttcttgtcc tgctgctatt ttttgaattt tttccaggcc agcttccctt tctgctaaag
    30181 ccatatagta aaatgaaatt gcccagggtt ctctcccagg taatgccatg ggtgttaaac
    30241 catgctttct gaattctttt actgcttcag ttaattcaga ataagtttct ggaattttga
    30301 tattatatct gtcaaacatt tctgtgttac aatatagccc cattatccag ccaaataacg
    30361 gcaatccata aattttatct tcataagtta aggcatcaaa agtgttactt agggcatttt
    30421 ctttcatttc ttgtacataa ggtgttaaat caaccgcttt tcctgactta actattggtt
    30481 cagcaaatcc atatccccaa acaaaaaata tatcaggacc ttcattaaca gctaatgccg
    30541 aaggtaattt agatttataa gtttcagttg tagttgttgt tatggtaatc tggacttcag
    30601 gatgacgctt catataatca tctaccagat tttgcaaagc ctttccggcg agaatgtcat
    30661 tattgcccca tatcgtccat atttctaaat gtatggggtt ttcgtcagtt tccgatatta
    30721 gcatctcatc tttttttcta tttgtattgc aagatgtcat taaaacacaa atgaacaaaa
    30781 tgatattaac cattatcctt aaagtttttt gactttgagt acagattttt atccccctct
    30841 gataaaatat ttataataac gtttaacgaa acttttgtcc aatactattt tagttgactt
    30901 aattgcgttt ttcaatataa cggagaatcc ccctccccat taaaatcagc ttttgtttta
    30961 ccaaaaccat aagttgtcca tctagtccat ttataaactg aaaacagata ttttttcttc
    31021 tactattgct gataaagctc ttaattcttc agccatctca accagatgct tattaagctc
    31081 tacttcctca ctggatgctg ctgctaaatt ttgcgtactt gcaaaatgtt ctctggaact
    31141 gttatttaat ctttccatta aaacaacaat ctcttttctg gacttttcaa tattggatat
    31201 taaatctttt acgccatcaa tttcctctac aacatagtcc atcgaatggt caattaactc
    31261 aaatttttct gatgtatctt taacagctat attatgctct tgtgtgaatt tttcagtact
    31321 gataactacc tttcttacat tttcaatact catactcata tgatttagtt tttcatcaat
    31381 atactgaacc gatgaacttg tctgttctgc taattttttt acttcctccg ctacaacact
    31441 aaatcctttt ccgtataaac ctgcacgcgc tgcttctact gctgcattaa gtgccagcag
    31501 gtcaatttgt tctgctatat attttattgt tgttgtaatc accgatattt cttttgactc
    31561 attgattaaa ccctctacta ccgccgaaac attttcaatc atctctttca aattttctgc
    31621 cttatccttc aaagttccaa cagattcttt accctgttca ctggaaactt ttacttcata
    31681 tattttttga ttcatatgat tgaaactttc aatgatatct tctatctcat cagataagct
    31741 aaatgccaca tgactacatg tttctattcc ttttacctgt gaatgtattt cttcctgaat
    31801 atcattaatc gtctgcgaaa attcttcaat tgtagcataa ttttcttcac ttgttgctga
    31861 taattcctga gataatttat aagtttcccc tgacattttt ttaatttcta atattaaata
    31921 tttcagtctt tctgccatat ccctgaaaca tcgtgctaaa atccctattt cgtcatttct
    31981 gcctacttta tactttatgt ttaattgacc ttcttttacc ttttcaattt gtttaataag
    32041 aagccccatt ggtttcgtaa tatctcttgt aattaacact gcagcaaaaa aagatataat
    32101 taaacaaaaa atgccgatta aaagcagttc aaatcccata gaagttactg gagctgttat
    32161 attttttaca ggtgttaaac caacaacaac ccaatctaaa tttttgcact tactaaaagt
    32221 aaccaacatt tcttctccgt caatttcttc aataaaccag gaagtatctt cctgaaaaac
    32281 tttatccagg tatctgtctt ttaaaatcat tccattattg tttaaatttt taggatccat
    32341 cactatattt ttatcctgac ctataacaaa gaaatatccg tcataatcca ttttaatatc
    32401 tttaaccaaa tcgtataaat attgttcttt tacgtttgcc attaaagtcc ccacactttt
    32461 aagtgatgta ctaacataca tatttttaaa taccgtcaat atccgcttag aaattccacc
    32521 tgtagcatca ttatcagttt cgtatgtatc aatccataat gaatttcttt cactctcttt
    32581 aaactttgta actgcatacg aagaaatcaa atcatacttt ggatccatta ctcttactcc
    32641 ggaagaaaaa atatcattat tctttgtaat aatataaatg tctgcaatct caacccttgt
    32701 attaatagca tcgttcatta aatgctgaat ttgattttta gtccttatct tttgattatc
    32761 atctaattta ctataatttt ctactaagct ccctatttgt tcatttcttg ataaagcagt
    32821 catattcctg tcaatttcca taaaagcaat atcaatactt ttgcttgttt gtttcataag
    32881 ttctatcaaa ttattcctgt tttgttcaat taaagaagaa gacattttac tataagataa
    32941 aattcctatt atagacaaag gaactataat taaaatggca aaggacagat taagttttgt
    33001 tccaattttt aaattcttat agaacatctg ccaaatcacc cttttcaata taaatcggat
    33061 agctatcctg ctaagatgcc tgctgcttaa atagtaaaaa acctagcctt ttaccgctcc
    33121 agctgttatt ccacttataa atttcttttg taaagtaaag aatatgatta gtattggtat
    33181 cgtagataca actccggcag ccatcaacag atcatatctg ttttgataat ttccaacata
    33241 taatcgaatc ccaaccggta ttgttgcaac atcaggcgtt gttgctaaaa cccaggcaaa
    33301 taaaagctca tcccaggcca ttaagaaaat atatatccct gtggcaataa ttcctgtact
    33361 ggacaaaggt aatattatcc tcataaatgc tccaaatgga ctacagccat ctatttgtgc
    33421 agcttcttct aattcttttg gaatgcttgc aaaaaaacct ctcaatatcc aaatactaaa
    33481 tggaataaaa aaagctgaat aggtaataat tattccatga taagtgttaa caatactaat
    33541 gcccagactt ctttgtattt ttatgaacat catgtaaaga ggaagcaaaa acataattcc
    33601 aggaatcatt tgagtggata atactgctgt tccaaataaa cctgaacccg gaaacttaaa
    33661 tcttgcaatg gaatagcctg caaaagttgc tatgataaga gcaataatcg ttgttatact
    33721 acacacaatc aaactattct tgaagtactt aaagaaatca atattattcc acatttcaat
    33781 ataattctcc cagtgcgtta tgtctgtaat tttccccgct gcaagattat tttgtgtcat
    33841 taaagaacta ataaacatcc atacaattgg gaatatagta aatataacaa ttataaagag
    33901 taatatattg gcagct
//
LOCUS       DAOSUD010000015        33011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_926_length_33011_cov_24.843428, whole genome shotgun sequence.
ACCESSION   DAOSUD010000015 DAOSUD010000000
VERSION     DAOSUD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 33011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33011
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_33011_cov_24.843428"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLG49_03495"
     CDS             <1..329
                     /locus_tag="PLG49_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16413.1"
                     /translation="RLYNLGLEPVMAIYSVNSVGTASDVYNPSIIIQLSTQIKCKILG
                     ELPAGWAYCWDDGMVFSLDYNDWCTYIDIVEAETRLKEVTKEFEAFLDTRDVESTRAV
                     ILLATG"
     gene            581..2173
                     /locus_tag="PLG49_03500"
     CDS             581..2173
                     /locus_tag="PLG49_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOQ16414.1"
                     /translation="MADKYCMYLRKSRKDDDIKSESIEETLARHEKALWELAERNGLT
                     VECVHREVVSGETIEARPVMQQLLKEVEQGMWRGVLVMEVERLARGDTSDQGRVAKAF
                     KYSETLIITPNKIYDPTNEFDEEYFEFGLFMSRRELKTITRRLQEGRKRSVLEGKYVG
                     NTPPYGYERVKIQGQKGYTLKEVPDEANAVRQIYKWYTEGETQPDGTNKRLGVSLIVR
                     RLNELKITPRKGDVWTTSSVRDILINPTYIGKVRWNWRRANKKLVDGKVTVERPRSDD
                     YIIADGLHKGIISEDTFNQAQELMKKNPPRPVGERNTVKNPLAGLIKCGVCGRRMVRR
                     PYGNGYPDTLMCPVTSCDNVSSHLHLVEERVIEGLKDWLAGYKLQWNISDKPKAAKSA
                     LDLARKSLIKLKKEIETIHTQQSKLDDLLEQGVYDIEKYKQRSQVLAERIKQAELDYQ
                     ELQSKIAEEEQREEARKVIIPKVEKLIETYYALPTPQAKNDLLKEVLEKVVYTKKQGG
                     RWHTSPDDFELVLYPRLPRSSP"
     gene            2261..2611
                     /locus_tag="PLG49_03505"
     CDS             2261..2611
                     /locus_tag="PLG49_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOQ16415.1"
                     /translation="MVYQLVKDLPESKIEGTPFAAHFANHGKSPYPHDASGVPFTATY
                     FNAKGDPIADIYENMAAEQKARATYEHLINLSDDPGVTDALRFLREREIVHFQRFGET
                     ARLLQEYMDSKRFY"
     gene            complement(2784..3206)
                     /locus_tag="PLG49_03510"
     CDS             complement(2784..3206)
                     /locus_tag="PLG49_03510"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOQ16416.1"
                     /translation="MIKRRDRMNYEELIKRANEAKENAYAPYSNFKVGAAVLTGTGKI
                     YTGCNIENVSFGATNCAERTAIFKAVSDGEKAIKAVAVMSSSGEFTYPCGICRQVIAE
                     FSEDAKIILGNSRGDIKVYSLDEILPCGFTKENSKNFE"
     gene            complement(3208..3909)
                     /locus_tag="PLG49_03515"
     CDS             complement(3208..3909)
                     /locus_tag="PLG49_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOQ16417.1"
                     /translation="MRKLKNFRDSKGKSKWLFNKKILKKSFLDLRRLFFSGLVSVLPI
                     AFTFFSISLVFNFADRLPRKIIATIIVFIFSLFNKKISLEEANVPGLGIIAAFFIIMV
                     TGYISTHLIGRRLLKAIEKWLLKIPLVSNIYTAFRQIMDTIFLKEKHTFKSVVLVQFP
                     CKGSYSIGFLTANSPSEVKNLKQESLVNVFIPTTPNPTSGFLLMVKEEDIYKLNMSVE
                     EAIKFIVSGGILSPE"
     gene            complement(3936..4439)
                     /gene="ybeY"
                     /locus_tag="PLG49_03520"
     CDS             complement(3936..4439)
                     /gene="ybeY"
                     /locus_tag="PLG49_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ16418.1"
                     /translation="MNILIESNDFQLNRNLKSIIKEVIIQALEEEGYPLSVEVSVTFV
                     DNESIRKLNKEYRNIDKETDVLSFPLLEFEESDEEKDVKILLDDLDAYKNPESGDIML
                     GDIIISYEKAKEQAEEYGHSLEREIGFLVAHSMFHLMGYDHMTKEEEEIMCKKQEKVL
                     ESIGLTR"
     gene            complement(4354..6555)
                     /locus_tag="PLG49_03525"
     CDS             complement(4354..6555)
                     /locus_tag="PLG49_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOQ16419.1"
                     /translation="MSKRRFIKIAIVLISFILTLSTVLTGEYFFKSEKIELGSVATKR
                     YIAPREIENTYATERKKEEVRKNIEPLYKEDSKVKEDALSKLELLFTKVQEARMYVEE
                     GNADNYSSNPEGDIINEENLVQEEESREERMERAIELLKAQSPILLLDQQFKALIEME
                     SGSIKELKNKCIDVLESTYDMGIREETKTKTILDVKSQFETFHTNEEINNLGYDIVAS
                     LIQPNNFIDEIATNKMIEERLKQVEPVMILQGQKIVDEGEIITEEIYSILDSLGFIQK
                     EFKEEAIKYVGVVLLICLLQIFIYIYIWFFNRSIIHSLRKTLLLFSIYCLIILIIRSM
                     TPLHFAWIPIPIAGMLLAVLVDVRIGIIINGYLAIIGALINKEGIDFLMFFIIVGTIF
                     PILVINIYKRSRILWMGFVAGIINGITLMSIEFFLIGEYSVEIIYSGYYGILSGILAV
                     IITAGSLPFWEVAFDLVTPIKLMELTNPDQHLLKRLLIEAPGTYHHSLIVANLAETAA
                     ADIGANSLLARVGGYYHDIGKLAYPQYFKENQMMENPHDYLEPTVSASILKQHIENGL
                     KIAAEYKLPKPVRNIIEQHHGTSRIQYFYYKALKDSSPDEVKKEDFTYQGVIPKTKEA
                     AVVMLADTVEAAVRSMDPQNKNKEECEKLIRKLIKDKLDEGQLNDSNLTIKDLETIVQ
                     AFMKVFNGMHHERVKYPEKEEKKNEHFDRKQRFSTKQESKKHNKGSHHTSA"
     gene            complement(6573..7532)
                     /locus_tag="PLG49_03530"
     CDS             complement(6573..7532)
                     /locus_tag="PLG49_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOQ16420.1"
                     /translation="MEQIEKTFQIPTNYISEIFGKLDENIKIIERAFRVEIINRGEYI
                     KVIGEEESVDKALRTLNQLLLLAQKNEEINIQNIEYIISLVNESIEKEYGEVSSDFIC
                     MTVHGKPIKSKTLGQKNYVDMIRNNTVVFGIGPAGTGKTYLAMTMAISALKNNEVNRI
                     ILTRPAIEAGEKLGFLPGDLQSKVDPYLRPLYDALYEIMGAEAFLKNMEKGIIEVAPL
                     AYMRGRTLDNSFIVLDEAQNTTPEQMKMFLTRIGYGSKAVITGDVTQIDLPSGKKSGL
                     VEAVKILKNIEGIGIALLTKRDVVRHPLVQRIIQAYEKYEKKR"
     gene            complement(7495..7680)
                     /locus_tag="PLG49_03535"
     CDS             complement(7495..7680)
                     /locus_tag="PLG49_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16421.1"
                     /translation="MLEEISDRGEVVSSEIKYYPKEDCLKAKAVFTILEHIEEVQAIN
                     REEFEDGANRKNLSDTN"
     gene            complement(7632..8732)
                     /gene="yqfD"
                     /locus_tag="PLG49_03540"
     CDS             complement(7632..8732)
                     /gene="yqfD"
                     /locus_tag="PLG49_03540"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOQ16422.1"
                     /translation="MFLSVWNYLRGYVTIEVSGFSVERFMNLASHKGIYLWDIKKYKS
                     KIQMKVSIKGFKLLKSCAKKTKCKIVIVEKKGCPFFIHKYRKRKVMAAGVLIFFGLLY
                     FLSSFIWLIEIKGNERIDTEELIKALENYGVKTGVLKMKVNADEVEGFLRDSFPDIAW
                     TAAEIKGTRLTIELTETVEKPLIVDRTTPCNLVAQRTGLIVSVTTRAGTPKVKANDVV
                     KKGDLLVSGEIIIKDDEEGTLKKYVHADADVLAKTRYELSFDQPLYYMEKQYTNNIKK
                     QHAIKIFDKELKLYNKEIDYSHYDKIIDQKQLSLSKYFVLPLYKITYEYREYTPVKHK
                     RTIEEAKQLAEEEIRKNCWKKSQTEGKLSAVK"
     gene            complement(8774..9031)
                     /gene="yqfC"
                     /locus_tag="PLG49_03545"
     CDS             complement(8774..9031)
                     /gene="yqfC"
                     /locus_tag="PLG49_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HOQ16423.1"
                     /translation="MKEKVTSMLELPKEVVMDYPVISMVGNEELHIENYKGILEYDTE
                     RIRICTTHGIIKVEGRSLLLKTMTMEEILIKGIIIKIEFLL"
     gene            complement(9295..9474)
                     /gene="rpsU"
                     /locus_tag="PLG49_03550"
     CDS             complement(9295..9474)
                     /gene="rpsU"
                     /locus_tag="PLG49_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOQ16424.1"
                     /translation="MSEVTVRENESLDSALRRFKRNCAKAGIMQEIRKREHYEKPSVR
                     RKKKSEAARKKNRHY"
     gene            complement(9583..10812)
                     /locus_tag="PLG49_03555"
     CDS             complement(9583..10812)
                     /locus_tag="PLG49_03555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOQ16425.1"
                     /translation="MKRPLLVFCVIFCFCLTACNIKINLKETNDEKLVMAANFNEEEV
                     IEEEKQLYTVKMRVVGDIMTHDEQLDVAYDKNNQTYHFDKQFELIKQYLSEADITIGN
                     LESTFGGPQRAYTGYPQFNTPDSLAQSLKDAGFDLLTTANNHSMDNSSEGVIRTLEVL
                     DQVGIEHIGTYASQEASETIKIKIINNISFAFLSYTYGTNGISVPKDKPYLIHLIDLE
                     EMKKDIEKAEELSPDFIVVFLHFGNEYERYPNKEQKYIVDELFNAGADIILGSHPHVI
                     QPMETRTITRADGSEEEVFVIYSLGNFISSQRSPKDPPRDAGIILNIDFEKSGNEKAR
                     IKNISFMPTWVQYSTVKVRGIIRVVSCTAPEEELKKHLLDFEIKRLKETKEYTLKHLL
                     GDKERKLEGDFYIYNSY"
     gene            complement(10922..13564)
                     /gene="alaS"
                     /locus_tag="PLG49_03560"
     CDS             complement(10922..13564)
                     /gene="alaS"
                     /locus_tag="PLG49_03560"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ16426.1"
                     /translation="MKFLGVNEIREKFLSFFESKDHLRMESFSLIPQNDKSLLLINAG
                     MAPLKAYFTGQEKPPKKRVTTCQKCIRTGDIENVGKTSRHATFFEMLGNFSFGDYFKK
                     EVIPWAWEFVTQVLEIPEERLYVSVYEEDDEAYDIWHKSVGLSAERIVRLGKEDNFWE
                     IGVGPCGPCSEIYFDRGEEFGCGRPDCAPGCDCDRFVEIWNLVFTQFDKDEEGNYNPL
                     PNPNIDTGAGLERVAAVMQGVTSIFDVDTVKHIRDKVCEIAKVKYQTDAKKDISIRVI
                     TDHIRAVTFMASDGIIPSNEGRGYVFRRLLRRAVRHGKLLGINKPFLEEIAVAVIETS
                     KEAYPELVEKKEYILKVLSVEEERFNETIDQGLDILKDFIAELKKSNNKVLSGKDSFK
                     LYDTYGFPIDLTMEILAEEGLSLDEEGFKKEMEIQRERARQAREETSYMGAEATIYTE
                     LNKEWKTKFVGYTDLTVSDVIIRAIIVDNQFVEKAEKGAEVSVIVDITPFYAESGGQV
                     GDKGIIKSNNGLIEIYDCIKVTGDNFAHIGKVIEGTVSTGEKAVLEVDKENRLATARN
                     HSATHLLHKALRELIGNHVEQAGSHVSPERLRFDFVHFSALSADEIMKVEEMVNKKIL
                     ENLPIVIQETSIDEARKMGAMALFGEKYGKEVRVVTMGDFSMELCGGTHLMNTAQIGL
                     FKIISETGVAAGVRRIEALTGQAALNYYRQQEALLHEAASLLKTNPNQISRRIKDLLN
                     EIKENQKELEKLRAKTAGELVGDILSRKKDVEGIPLITARLDDLDMAALRNMGDNLKN
                     KMGSGLIVLASGKDSKVSFVVMATEDLVKKGVHAGNLIREIAQIAGGSGGGRPNMAQA
                     GGKDLSKIDEALNRTEEILKKQLN"
     gene            complement(13598..14599)
                     /locus_tag="PLG49_03565"
     CDS             complement(13598..14599)
                     /locus_tag="PLG49_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOQ16427.1"
                     /translation="MKKIAFVFLSLSVILLSMYSYKLLNEISARNVYHEEDSTGKISK
                     TEGNNETEGEQVVDRKTKIIEDSDLEAVFDYFRRQKDHSLILVNKWNLLSENYQPTDI
                     KDAAGIVPSTKEQMLLQKEAYEALITMFNGAKNEGIEGLFVVSGYRPYSYQSQLYNAK
                     VNAFSSQYDLETAKEKASEIVAIPGTSEHQTGLAIDFSSAKLLKTADPLVEEFKNTPQ
                     GEWLYNNSWKYGFVLRYLPEKKEITGIISEPWHFRYVGIPHAQYMTLNHLCLEEYIEH
                     VKEEKMVIFEDYFGNKYLIGFVQKENHDILLSEEFSLEKVMNVSEIGEEEYIITLKL"
     gene            complement(14807..14968)
                     /locus_tag="PLG49_03570"
     CDS             complement(14807..14968)
                     /locus_tag="PLG49_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOQ16428.1"
                     /translation="MIINKFTAGMIIGGIAGAMGFKMMNSSRRERKKMMKSGRKLIER
                     AGEIFEELR"
     gene            15106..16683
                     /locus_tag="PLG49_03575"
     CDS             15106..16683
                     /locus_tag="PLG49_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HOQ16429.1"
                     /translation="MKNKFFTFLLAVVLTCFYCSPVYGVNASATNIVNPYQAISYSQM
                     MEYLKRLENQFEDLIEMEIIGKSLDNRDIVLVKLGKGDTLIHINGSMHARERITTNII
                     LKNIEDYCNAYRNNSSISGYNVKELLDQVTIYYVPMVNPDGVDYTISGKASIRDPLLK
                     SNIDSIYHSPQTGWNHTSNRWKANIRGVDLNRQWNSGWNKNLKYDPGKPADAYYKGPE
                     PLSEPEAQAIYQLSIENPFMIHVAYHTQGKIFYWYKNQTGTLYEQSLNISKKIANLTG
                     FKAYPSLNYKGYFESYAGYTDWTIEELKKPGFTMEFATNPYSEKNFDEIYKPAKALGL
                     LFAREALNLKSVYNTEVYINQKLTQVFENDANASAFITKYVTKDSDIKIIKNGEVLYA
                     YEPYSPEKLLIMANDFANLIYNYDYRNNNEDWPALLAQYIPDTGFSMNHVILTSEELN
                     DMRKEEKLQITGSFRSDIHSITKGDSNIYTIKGQVDYRYLSDDSEDSLSKNIMISIYI
                     DNHSKAQIISLIEENIE"
     gene            complement(16717..17964)
                     /locus_tag="PLG49_03580"
     CDS             complement(16717..17964)
                     /locus_tag="PLG49_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOQ16430.1"
                     /translation="MAVLRPFKAFRPKEDLVHKVAALPYDVMSSDEARKMVIGNPYSF
                     LHIDKAEIDLDPSIDLYDKRVYEKAKDNLNKMIQEGIYLMDEKPCYYIYKLVMEGRAQ
                     IGIVGCTSIDDYVNNVIKKHELTREDKEQDRINHVDYCNANTGPIFLTYRSKKEINQL
                     VDNWMNNHEAVYDFSAEEQVRQMVWVIDDPKVIEAISHYFSEINCLYIADGHHRAASA
                     VKVGLKRRKEAGSFTGEEEFNFFLAVLFPDEQLHIMEYNRVVKDLNGLSEEEFFDRIR
                     EKFIVEKIEDNQAYKPKAKHEFGMYINKSWYRLIAKEGSFNQEDPVERLDVSILQDNL
                     LAPVLGIKDPRTDNRIDFIGGIRGLKELERRADTDMKLAFSMYPTSIEDLMDIADAGK
                     IMPPKSTWFEPKLRSGIFIHSLE"
     gene            complement(18020..19780)
                     /locus_tag="PLG49_03585"
     CDS             complement(18020..19780)
                     /locus_tag="PLG49_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOQ16431.1"
                     /translation="MSDVKVTIDGKEVEVPKGSTVLQAAHKIGIDIPTLCHLDLHDIK
                     MVNKAASCRICVVEVEGRRNLAPACATPVFDGMVVRTNTIRVLNARRIVLELLLSDHP
                     KDCLVCSKSGNCELQDLAERFGLRKVRFEGAQSTYRKDYSKTIIRDMDKCIMCRRCET
                     MCNDVQTVGALSGINRGFNAVVAPAFEVNLEDSVCTHCGQCVAVCPVGALHEENMTWS
                     VVQALADPDKVVIVQTAPAVRAALGEEFGLEPGTLVTGRMVAALRRLGFDYVFDTDFA
                     ADLTIMEEGAELLDRIKRYLDGDKTVKLPILTSCCPGWVNFIESQFQDLLDVPSSAKS
                     PQQMFGSIAKSYFAEKLGIPREKMVVVSVMPCLAKKYEASREEFSVDGNPDVDISIST
                     RELAHLIKKANINFNELEDEEFDLPLGESTGAAVIFGTTGGVIEAATRTAYELYTGET
                     LEKVDFQQLRGMEGVRVATVKMGNLDLNIGIAHGLGNARKLLEEVKSGNPRNLHAIEI
                     MACPGGCIGGGGQPYHHGNMEIIKKRAEAIYREDAGKPIRKSHENPVIKELYETYLGH
                     PMSERAHHLLHTKYTPKDRV"
     gene            complement(19802..21595)
                     /gene="nuoF"
                     /locus_tag="PLG49_03590"
     CDS             complement(19802..21595)
                     /gene="nuoF"
                     /locus_tag="PLG49_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ16432.1"
                     /translation="MSRYKMHVLVCGGTGCVSSNSIQIVENLKKYLKENGIEDEVQVL
                     KTGCFGFCEKGPIVKILPDNTFYVQVKPEDAEIIVKEHLIKGRKVERLLYKEPNTQTA
                     IDDSKHMDFYKKQMRVALRNCGFIDPDNIEEYIARDGYQALGKALTEMTPEQVIEEVK
                     KSGLRGRGGGGFPTGLKWEFAYKNKSDQKYVVCNADEGDPGAFMDRSILEGDPHSIVE
                     AMAICGYAIGANKGLVYIRAEYPLAVKRLKKAIEDAREYGLLGKNILDTGFDFDIEIK
                     YGAGAFVCGEETALIHSMEGERGEPTTKPPFPAESGYWGKPTNVNNVETFANIPVILL
                     KGADWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREVIFDIGGGIKGSKKFK
                     AVQTGGPSGGCLTEKDLDTPIDFDNLVAKGSMMGSGGMIVMDEDDCMPSVAKFYLEFT
                     EDESCGKCTPCRIGTKRLSELLDKITKGQGTMEDLEMLKTLSETIKDTALCGLGQTAP
                     NPVLSTIDTFWDEYVAHVKEHRCPAGQCSALLQYFILEEKCIGCTACSRVCPVGAISG
                     KVKSTHVIDQDKCIKCGACYEKCKFSAIIKK"
     gene            complement(21618..22016)
                     /locus_tag="PLG49_03595"
     CDS             complement(21618..22016)
                     /locus_tag="PLG49_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOQ16433.1"
                     /translation="MGKIKSLEELKQLREQYKSKVDIREKGDQMEKMILVRVAMATCG
                     IASGARDVMDAFIDEVANRKLDNVVVSQTGCMGYCYAEPTVEVTIPGKEPVVFGEVNK
                     DRVKEIVDKYIIHGELLDGIIPVTHKTVDD"
     gene            complement(22037..22528)
                     /gene="nuoE"
                     /locus_tag="PLG49_03600"
     CDS             complement(22037..22528)
                     /gene="nuoE"
                     /locus_tag="PLG49_03600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOQ16434.1"
                     /translation="MANCETCKNKLSESCFRELEAFIDGLEDKNGAVISVLHKAQEIF
                     GYLPREVQQFVAKKLEIPVSKVYGIVSFYSFFTMVPKGEHPISVCMGTACYVRGAEKV
                     LDEIKRTLNIEVGETTPDGKFSLDALRCVGACGLAPVVLVGDKVYGRVTPDQVKSILK
                     EYE"
     gene            complement(22700..23404)
                     /locus_tag="PLG49_03605"
     CDS             complement(22700..23404)
                     /locus_tag="PLG49_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ16435.1"
                     /translation="MKYYYDFHIHTALSPCGDEDMTPNNIVNMALLKELDMIAITDHN
                     SCENVEAVMKVAQGTNLLVIPGMEIESSEEVHMICLFPSLEAAKAMQEIVYNSLPPLK
                     NRTDIFGKQLILNEYDDVIGENDRLLLTATSLSVYDLVKEVERQSGVVYPAHIDRKSY
                     SIISNLGWIPEDLGVKTIEISRYAEFDKYISEYSTFNLIQSSDAHYLQDISEREHFIE
                     LAEKSPAKVIEYLNKR"
     gene            complement(23416..23751)
                     /locus_tag="PLG49_03610"
     CDS             complement(23416..23751)
                     /locus_tag="PLG49_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOQ16436.1"
                     /translation="MKVKDICEKLDLSVVAGETALENEISTVYVGDLLSWVMAHAKAS
                     SIWITIQSHVNIVAVASLLNLSCIIVAEGAKVDEETVEKANNEEIPILTTNKDAYCLI
                     KELIHLGIE"
     gene            complement(23767..25080)
                     /locus_tag="PLG49_03615"
     CDS             complement(23767..25080)
                     /locus_tag="PLG49_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOQ16437.1"
                     /translation="MIEYLHSVTLQEDKCVGCTDCIKRCPTEAIRVRHGKAKIIDERC
                     IDCGLCIKVCRNRAKKAMTDKLDCLSEYKYKVAIPAPTLYAQFKDIYDVNVILTGLKQ
                     LGFDEVFEVSRAAEIVTEESKKLLKSSNLKKPVISSACPVIIRLIQIRFPELIEHILP
                     VISPMEVAARIVKNDLIKRGIDEKDIGVFFISPCAAKATNARNPIGIEKSHVDKVLSI
                     KEVYKKLAGTLKRLDKIENLRTSSYDGIRWAMSGGEGKGLEIDNFIAVDGIENVIKVL
                     EEVENGKLVNVNFIEGLACTGGCLGGPLTVENSFVAKNKLKKALKNTLEEKAGDCANL
                     HTEDITLFWNKEVLSKPVMSLDEDMNIAIKKMEELERIYEQLPQIDCGSCGAPNCRAL
                     AEDIVRNNANIEDCIFMLRKKVREMAEDMVALAQKIPASLNNKDF"
     gene            complement(25081..25506)
                     /locus_tag="PLG49_03620"
     CDS             complement(25081..25506)
                     /locus_tag="PLG49_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOQ16438.1"
                     /translation="MKLNFEVDGDDFLLAGEASSQVKKVLKQLGVASDIIRRIAIAMY
                     EAEINMVLHANGGMVDVDISPEKIYIVFQDTGPGIEDIELAMQEGYSTASDKIRELGF
                     GAGMGLPNMKRYSDDLKITSELGKGTKVEIIVYLNREGD"
     gene            complement(25490..25867)
                     /locus_tag="PLG49_03625"
     CDS             complement(25490..25867)
                     /locus_tag="PLG49_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOQ16439.1"
                     /translation="MKLEEVKQILNAEVLVGEDRLDREVLLACGSDLMSDVLAFVKEK
                     VLLLTGLTNPQVIRTAEMMDIKAIVFVRGKQPDPETIQLAEKKNIVILKTKYPLYIAC
                     GLLYSNGLTGDEKKQEEGYEVKF"
     gene            complement(25920..27086)
                     /locus_tag="PLG49_03630"
     CDS             complement(25920..27086)
                     /locus_tag="PLG49_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOQ16440.1"
                     /translation="MDIEKLEKLLKQPEGFKLDFKECLRLDTDSDKKEFVKDVCAIAN
                     SSGGRGHIVFGVKDKSREVVGVDLEPFDEERIQQIICNRCDPPVPIKVDIIDYHGKNV
                     AVITIYKSNQRPHQVRQTGTFYIRRGSTTDVARRHEIANMLQENGLTSSEQTLCRKVP
                     ISELDEEMLKQYILGKDNIFEVNKDLYLEGLGIIGRDSNKSEYHPTMGGLLLFGKNPQ
                     NYLPHTGIRIDYDGRVIPVRGNILEMLDKAEAIISDIFKDYDYPVQSVFDALSNAVVH
                     RDYWDMSREITVKIEEDQVSISNPGAIWYRGSIKKIMQDQNPPRRNPWLYQRLLLMDK
                     KERFLNYGLGINRIKKPFANVGQVRVYSFLNTNLFKIVLPGRKKFPKLKADKSQ"
     gene            complement(27215..27898)
                     /locus_tag="PLG49_03635"
     CDS             complement(27215..27898)
                     /locus_tag="PLG49_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ16441.1"
                     /translation="MSVKQKILIVDDDVHIAELISLYLNKEGYETKEVYSGDKAVKAF
                     LSFAPNLVLLDIMLPEMNGYDVCKEIRKISNIPIVMLTAKGETFDKVLGLELGADDYI
                     VKPFEPKELIARVKAVLRRYEPKEDNSKKIVIPNLTIDLGNYSVIYHGKQMDLPPKEL
                     ELLYFLASNPNQVFTREQLLDRIWGYDYVGDTRTVDVHIKRIREKFDEKESWSIKTVW
                     GVGYKFDIQ"
     gene            complement(27957..28640)
                     /locus_tag="PLG49_03640"
     CDS             complement(27957..28640)
                     /locus_tag="PLG49_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOQ16442.1"
                     /translation="MGIFERMVTLIKSNINDLIDKAEDPEKMLNQLILDMQEQYTEAK
                     KQVAQAMADEKKLERQYEEQLKLQEEWAKKAELAVQQQNDELALKAIKRKNEHAKLAE
                     EYKVHLDKQKEITDKLKNSLVELNNKIEEAKRKRELLLARAQRAKAQETVNKTYADLN
                     DTSAFESFERMEAKIEAMEDKALASESLAMEVTGDSLESEFEKLESSMQDDAALKELE
                     ALKAKLSNN"
     gene            complement(28857..31238)
                     /locus_tag="PLG49_03645"
     CDS             complement(28857..31238)
                     /locus_tag="PLG49_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOQ16443.1"
                     /translation="MESRSLKTLEYHKIIDKLVNYAVSPMAKEIAGELIPTDDYNEII
                     QRQKETSYALTMMIKKGTIPLGGFKDIRSSIKRVDIGGILTAAELMHMADFLYVCKKV
                     KNYYKDDKKEESYGSLDALFEGITTLPALEREISRCILSEEEIADDASPALSNIRKEI
                     KGMNEKIKNQLQKIIYSSANQAKLQEAVITIREDRYCVPVKQEYQNSFPGIVHDQSAS
                     GATLFIEPMVIVQMNNLLRELKIKEKNEIEKILGQLTEMVAQNIEIIKNNLELLIRLD
                     FIFAKAQLSLEMKGVEPRFNHEGRIHIKKGRHPLLDPKTVVPIDIYLGDQFTTLLITG
                     PNTGGKTVSLKTLGLFVLMGQAGLHIPANDSSELAVFDNVFADIGDEQSIEQNLSTFS
                     AHMTNIVDILKKVTPDSLVLFDELGAGTDPTEGAALAMAILDYLLKMKVRTAATTHYS
                     ELKVYALSTDGIENASCEFDIETLRPTYRLLIGIPGKSNAFAISKRLGLPEYIIDNAA
                     LFIAKKDKQFEDLIADLEISKKTAILEQNRAQEYRREAEALKKIVEEQKEKLDKQKEK
                     IIHEARQEARKILQEAKDEADKIIKEIQKSAREAQKVIDYRDMEEARGSLRERLKELD
                     EEILKPGSKKITGKTPEKLKKGDKVFVTSLNQKGIVIIPPNANGDVTVQAGIMKLSVH
                     LSDLILDDEEENQDKKIKATRTTAAKSKTMNIGPELDLRGQTVDEAIINTDKYLDEAF
                     LSSLSQVTIIHGKGTGALRQAIHQLLRRHPHVKSYRLGKYGEGETGVTIVELK"
     gene            31350..31928
                     /locus_tag="PLG49_03650"
     CDS             31350..31928
                     /locus_tag="PLG49_03650"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOQ16444.1"
                     /translation="MAFINPKDLYYEIFSSIQSNFSMPIKISSNTKVNSMNFKVVLEK
                     ELNAAFDHQKLSLEEISQRIENAIAEASKKYNLSPELIKAVIKQESNFNPNALSSAGA
                     QGLMQLMPATAKMLGVENPWDIEDNIDGGSRFLKDMLVRFNGNLSLALAAYNAGPGNV
                     EKYNGIPPFTETQNYVPKVLAYKKQYEAFKKE"
     gene            complement(31967..32854)
                     /locus_tag="PLG49_03655"
     CDS             complement(31967..32854)
                     /locus_tag="PLG49_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOQ16445.1"
                     /translation="MIGLRRVLTVAFACGVFSLATMTDVYGQTLALVNKDGVEVWDKL
                     DETKEVIVTVDKDEELEIVGTEGDCYIVLLDDESKGYVLKDDLNIKSMDAISTGDRVN
                     IRVEPNTSCKVLGQVNRSDPLVVTGKTGNWYKIDYKGQEAYIFGDYVKVLDKNVLADK
                     KEEAVTSYNKNADEIITYAKQFIGTPYRYGGTDLTRGVDCSGFTQSVMKKFGISLSRS
                     SSSQANDGYYVAKSDLQPGDLVFFDTSGPNNGAISHVAIYIGNNSIIHSECSRGVTID
                     SLSEDYYARRYVKAVRVLK"
BASE COUNT         9986 a         6788 c         4808 g        11429 t
ORIGIN      
        1 acagacttta taaccttgga cttgagcctg taatggcaat ttacagcgtc aatagtgtag
       61 gcactgcttc tgatgtttac aatcccagta tcataatcca gttatctacg caaataaagt
      121 gtaagatact gggagaatta cccgcaggct gggcttattg ttgggatgat gggatggtgt
      181 tttcgctgga ttataatgac tggtgtacct atattgatat tgtggaggca gaaacacgct
      241 taaaagaagt taccaaggag tttgaggcgt ttctggatac aagggatgtt gagagtacaa
      301 gagctgtcat acttttggct acaggttaag gaagtagagg ttgcttacta ataaaaacct
      361 actgcgaaat aattaccttc tatataatac tggttaaatt ggcctgcgat ttttcttcgt
      421 aggctttctt gatgaaataa aaaatttttc actcttaaaa tttactggta atattggttg
      481 caggcataat aaaatcgtgt aagttttgtt atcatttatg gtataatatg attgcacata
      541 tataggtgat acggctttaa tgattttagg gagtgatgtt atggcggata aatattgtat
      601 gtatcttcgt aaatcccgca aggacgatga cataaaatct gaaagtatag aggaaacact
      661 ggctcggcac gaaaaagctc tatgggaact tgctgagcgt aatgggctta ctgtggagtg
      721 cgtccaccgt gaggtagtgt ctggtgaaac tattgaagca cgtccagtca tgcagcaact
      781 gcttaaagag gtagaacagg ggatgtggcg tggagtgctt gttatggagg tagagcgttt
      841 agcccgtggt gatacatcag accaagggag agtggctaag gctttcaaat atagcgaaac
      901 tcttataata acccctaata aaatctacga cccgaccaac gaattcgacg aggagtactt
      961 tgagtttggt ttgttcatgt caagacgtga acttaagact atcacacgca ggcttcaaga
     1021 aggtcgcaaa aggtcagttt tagagggtaa atatgttgga aacacgcctc catatggcta
     1081 tgaacgggta aagatacagg gacaaaaagg ttatactttg aaagaagtac ctgatgaagc
     1141 taatgctgta aggcagattt ataaatggta caccgaagga gaaacacagc cagatggcac
     1201 aaataaaagg cttggggtat cgctgattgt gaggaggctg aacgagttaa agataacacc
     1261 aagaaaaggc gatgtttgga ctacctcaag cgtcagggat atactaataa atccgacata
     1321 tatcgggaaa gtcagatgga attggcgtag agcaaataaa aagctggttg atggaaaagt
     1381 aactgtggaa agaccccgct cagatgatta cataattgct gacggattac ataaagggat
     1441 aatcagtgag gatactttta atcaagcaca agagctgatg aagaaaaatc ctccaagacc
     1501 agtaggagaa agaaacactg ttaaaaaccc attggctggg ttaataaaat gtggcgtatg
     1561 tggacgtcgc atggtaagac gaccttatgg taacggttat cctgatacct taatgtgtcc
     1621 tgtaacatcc tgtgataatg tgagttcaca tctacattta gttgaagaaa gggtcattga
     1681 aggacttaaa gattggttgg ccggttataa gctacagtgg aatatttcag ataagccaaa
     1741 ggcagctaaa tcggctctgg atttggctcg caaatcgcta ataaaattga aaaaagaaat
     1801 tgaaacaatt cacacgcagc aatccaagct cgatgacctt ctcgagcaag gtgtatacga
     1861 tattgaaaaa tacaagcaaa ggtcgcaggt acttgctgaa cgaataaaac aagccgaact
     1921 ggattatcaa gaactacagt ctaaaatagc agaggaagaa cagcgagaag aagcccgtaa
     1981 agtcataata ccaaaagtcg agaaactgat tgaaacttat tatgcactgc ctacaccgca
     2041 ggctaaaaac gacctgctaa aagaggtatt agaaaaagtt gtatatacca aaaagcaagg
     2101 tggtcgatgg catacgtcac ctgatgactt tgagttagtt ttatatcctc gtttacctcg
     2161 ctctagccct tgataaccaa gggtttaggg caatttcatt tgctatcact gatatcttag
     2221 tggaactaaa gaacttgctc actgggaaat cattggtacc atggtttatc aattggtaaa
     2281 agatctccct gaatctaaaa ttgaaggaac accctttgca gcccattttg caaatcatgg
     2341 taaaagtcct tacccacatg atgcatcagg cgtacctttt acagccacct actttaatgc
     2401 aaaaggggat cctattgcag atatttatga aaatatggct gcagagcaaa aagcaagagc
     2461 aacttatgaa cacttgatta acctgagtga tgacccaggt gttaccgatg ctctgcgatt
     2521 tttaagggaa agggaaattg ttcacttcca aagatttgga gaaacggcaa gattacttca
     2581 ggaatatatg gattcaaaaa gattttatta atttttgata aagcaaaaac cctgctgttg
     2641 cagggttttt gtagaattaa caagggggga agtacttata tgaatcctct atgactttac
     2701 ttaagtttat tttaatacga tactctatca atgtccattt catttatgta tcatttatat
     2761 ttcatttata tttcaaaatc tatttattca aagtttttgc tgttctcttt cgtaaagcca
     2821 caaggtaaaa tttcatccag ggaataaact tttatatccc ccctgctgtt tcctaagata
     2881 atttttgcat cttcagaaaa ctcggcgata acttgtctgc aaattccgca aggataagta
     2941 aactccccgg aactgctcat aacagccact gctttaattg ccttttcgcc atcagagact
     3001 gccttaaaaa ttgccgtcct ttctgcacaa ttcgttgcac caaaggaaac attttcgata
     3061 ttacagcctg tatatatttt ccctgttcct gttaatactg ccgcaccaac tttaaagtta
     3121 gaataagggg cataggcatt ttctttagct tcattggctc ttttgattaa ttcctcataa
     3181 ttcatcctat cacgcctctt tatcatatta ttcaggagat aaaatacctc ctgaaacaat
     3241 gaatttgatt gcttcttcaa cagacatatt taacttataa atatcttctt cttttaccat
     3301 gagtaaaaag cctgatgtcg gattaggagt tgtaggaata aagacattca ctaaactttc
     3361 ctgttttaaa tttttaactt cagaaggtga atttgccgtc aaaaatccaa tgctatagct
     3421 tcctttacaa ggaaattgga caagtacaac acttttaaag gtatgcttct cttttaaaaa
     3481 aatcgtatcc attatctgcc ggaaagcagt ataaatattt gaaaccagag ggatttttaa
     3541 aagccatttt tctatggctt ttaaaagcct cctgccaatt aaatgcgttg aaatatagcc
     3601 tgttaccata ataataaaga aagctgctat tatccctaaa ccaggaacat ttgcctcctc
     3661 aagagatatc tttttattaa atagagaaaa gataaagact attattgtgg ctataatttt
     3721 cctgggcaac ctgtctgcaa aattaaaaac caggctaatt gaaaaaaagg taaacgctat
     3781 gggcaaaacc gatacaagac ctgaaaagaa taagcgtctt aaatctaaaa aacttttctt
     3841 aagaattttt ttattgaata accatttgga ttttcccttg gagtctcgaa agttttttaa
     3901 ctttctcatt ttgggtctcc tttgctgtca aaatactatc ttgtaagacc aattgattca
     3961 agtacttttt cctgcttctt acacattatt tcttcttctt cttttgtcat atgatcatag
     4021 cccatcaggt gaaacatgct atgagctact aaaaatccaa tctctctttc taaagaatga
     4081 ccatattctt ctgcctgctc ttttgctttt tcgtatgaga taataatatc tcccaacatt
     4141 atatctccac tttcaggatt tttatatgca tccaaatcat ccaggagaat ttttacatct
     4201 ttctcttcat cagactcttc aaattctaat aagggaaaag aaagaacgtc tgtttctttg
     4261 tctatattcc tgtattcttt gtttaacttt ctgatgcttt cattatcaac aaaagttacg
     4321 ctcacttcta cagaaagagg ataaccttct tcctcaagcg cttgtatgat gacttccttt
     4381 attatgcttt ttagattcct gtttagttga aaatcgttgc tttctatcaa aatgttcatt
     4441 tttcttctcc tctttctcag gatattttac tctctcatga tgcataccat taaatacttt
     4501 cataaaagcc tggacaatgg tttccaaatc tttaatggtg agattgctgt catttaattg
     4561 accttcatct aatttatctt taataagttt tctaattaat ttttcacatt cttctttgtt
     4621 tttattttgt ggatccatgg aacgcacggc tgcttcaacc gtatcagcaa gcataaccac
     4681 tgctgcttcc ttcgttttgg gtatcacacc ctgataagta aaatcttctt ttttgacttc
     4741 atcaggagaa ctgtctttta aagctttata ataaaaatat tgtattctgc ttgtcccatg
     4801 atgctgctca atgatatttc ttactggctt aggcagttta tactctgcag caatcttaag
     4861 accattctca atatgctgct taagaatact tgcacttacc gtaggttcta aataatcatg
     4921 gggattttcc atcatctgat tttctttgaa atattgagga taagccaatt tacctatgtc
     4981 atgataatag cccccaaccc tggctaacaa ggagtttgct ccaatatccg ctgcagccgt
     5041 ttccgccaga tttgccacaa ttaaactgtg gtgataagtt cccggagctt ctattaacag
     5101 tctttttaac aaatgctggt caggattggt aagctccatc agtttaatcg gagttaccaa
     5161 atcaaaagca acttcccaga atggcaggct gcctgcggta atgataaccg ctaaaatccc
     5221 acttaatata ccataataac ccgaataaat aatctccaca gaatactcgc ctattaagaa
     5281 aaattcaata ctcatcaaag taatgccgtt gattatacct gccacaaaac ccatccataa
     5341 aatccggctt cttttataaa tattgatcac aagaatagga aaaatggtgc cgacgataat
     5401 aaaaaacatc aggaaatcta tgccttcttt attaattaaa gccccaataa tagcaagata
     5461 accattaata ataattccaa ttcttacatc cactaaaacc gctaaaagca ttccggctat
     5521 gggtatagga atccatgcaa aatgcaaagg cgtcatggag cggataataa gaataataag
     5581 gcaatatatt gaaaacagta aaagtgtctt tcttaaacta tgaatgatag agcggttaaa
     5641 aaaccatatg taaatataaa taaaaatttg caacagacaa ataagtaaaa cgacaccaac
     5701 atacttgata gcctcttctt taaattcttt ttgaataaag ccgagactgt ctaatatgct
     5761 gtaaatttcc tccgttatta tttctccttc atcaacaatt ttttgtccct gaagaatcat
     5821 gaccggctct acttgtttaa gcctttcttc tatcattttg ttcgtagcaa tttcatcgat
     5881 gaaattatta ggctgaatca gggaagcaac aatatcgtat cctaaattat tgatttcttc
     5941 attggtatga aaagtctcaa actggctttt tacatccaat atggtcttgg tttttgtttc
     6001 ttcccttata cccatatcat atgttgattc caatacatca atacacttgt tttttaattc
     6061 cttaatactt cctgattcca tttctattag cgctttgaat tgctggtcca gtaaaagaat
     6121 aggagactgg gcttttaata attctattgc tctttccatt ctttcttctc tgctttcttc
     6181 ttcctggact agattttctt cattaattat atctccttcc gggttagaag aataattgtc
     6241 tgcgtttcct tcctccacat acatcctggc ttcctgaacc tttgtaaata ataattctaa
     6301 tttagaaagt gcatcctctt taacttttga atcctcttta tataaaggtt caatgttttt
     6361 ccggacttct tccttttttc tttctgtggc ataagtattt tctatttccc tgggggcaat
     6421 atatctttta gtggcaacag aacctagctc aattttttca cttttaaaaa agtattcccc
     6481 cgttaaaact gtagagagcg ttaatataaa agagattaaa acaatagcaa ttttaataaa
     6541 acgccttttg ctcaacgaca tctcctacct tttcatcgtt ttttttcgta tttctcatag
     6601 gcctgaataa ttctttgtac caatggatgt cttaccacat ctcttttcgt taaaagagca
     6661 attccaatac cttcaatatt tttcagaatc tttacagcct caactaaacc agatttcttt
     6721 ccggaaggta aatcaatctg agtcacatca ccagtaataa cagccttaga accataacca
     6781 atacgggtta aaaacatttt catttgttca ggcgttgtat tctgtgcttc atcaagaaca
     6841 ataaaagaat tatccaaagt acggcctctc atataagcca gaggagctac ttcgataata
     6901 cccttttcca tattttttaa aaacgcttct gctcccatta tttcatataa ggcatcatat
     6961 aaaggtctga gataaggatc cactttactt tgcaaatctc ctggtaaaaa acctaatttt
     7021 tctccggctt ctattgctgg cctggttaaa ataatccgat tgacttcatt atttttaagg
     7081 gctgaaatcg ccatagtcat ggctaaataa gttttccccg ttcctgcagg tccaatccca
     7141 aagactactg tattatttct tatcatatca acataattct tttgtcctaa agttttactt
     7201 tttataggct ttccatggac cgtcatacaa ataaaatcag aactgacttc cccatattct
     7261 ttttcaatac tttcgttcac cagagaaata atatattcaa tattttgtat attaatttcc
     7321 tcattttttt gcgcaagtaa aagcaactga ttcagcgtcc gcagagcctt atcaacactt
     7381 tcttcttctc ctataacttt aatatactct ccacgattga taatttcaac tctaaatgct
     7441 ctttcaataa tcttaatatt ttcatccagt tttccaaata tctcagatat ataattagtt
     7501 ggtatctgaa aggttttttc tatttgctcc atcttcaaat tcctccctgt taatggcttg
     7561 aacttcttca atatgttcca gaatagtaaa gactgctttt gcctttaaac aatcttcttt
     7621 tggataatat tttatttcac tgctgacaac ttcccctctg tctgagattt cttccagcaa
     7681 ttttttctaa tttcttcttc ggctaattgc ttagcctctt caatggttct tttatgtttt
     7741 acgggagtat attcccggta ttcgtaagtt atcttataaa ggggtaaaac aaaatactta
     7801 cttagagata actgcttctg atcgattatt ttatcataat gactataatc gatttcttta
     7861 ttatataatt taagttcctt atcgaaaatt ttaatcgcat gctgcttttt aatattattt
     7921 gtatattgtt tttccatgta atataaaggc tggtcaaaac ttaactcata gcgggttttt
     7981 gcaagaacat ctgcatctgc atgaacatat ttttttaatg ttccttcttc atcatctttt
     8041 atgataattt ctccactgac cagtaaatct ccttttttta ccacatcatt ggcttttacc
     8101 tttggtgttc ctgcgcgtgt agtaacgctt acaatcaatc ctgtcctctg ggcaacgaga
     8161 ttacaaggtg ttgttctgtc tacaattaaa ggtttttcca ctgtttccgt cagttcaatg
     8221 gtaagcctgg ttcctttaat ctctgcagcg gtccaggcaa tatcaggaaa actgtccctt
     8281 aaaaagcctt ctacttcatc tgcattgact ttcatcttca atacacccgt ttttacgcca
     8341 taattttcta aggcctttat caattcctca gtatctattc tttcattacc tttaatttca
     8401 atcagccaga taaatgacga taaaaaatat aagagtccaa aaaaaatcag cacacctgct
     8461 gccatcactt ttcgctttct atacttatga ataaaaaaag ggcagccttt cttttctaca
     8521 ataacaattt tacatttggt ttttttcgca cagctcttta gtaatttgaa gccctttatg
     8581 cttactttca tctggatttt acttttgtat tttttaatat cccataaata tattccctta
     8641 tgagatgcta aattcataaa tctctcaact gaaaaaccag atacctctat agtaacatag
     8701 cctcttaaat aattccatac agataaaaac ataaaggccc cccaatttta ttaataaaca
     8761 ttatatcgct taattataat aaaaattcaa tttttataat gatacctttt attaaaattt
     8821 cttccatagt catggtcttt aataataaag accttccttc aactttaata attccatggg
     8881 tcgtacatat tcttattctt tcagtatcat attccaggat acctttataa ttttctatat
     8941 gtaattcttc atttccaacc attgaaatga ctggataatc cataacaact tctttaggga
     9001 gctcaagcat actggttact ttttctttca aactgactgg ctgaacaagt tcttcttttt
     9061 tttgtttttt gtctttttat ttttcataaa aattgtccct ccctctcctt aattaatatg
     9121 ccaaaaaaag tattttaaga catattaata tatgatgaag ggattttctc ataaaaacaa
     9181 aataagctgc gtcaatacgc aacttatttt aaattttatt tattaaattc atttattatc
     9241 tgctggatta ctatcaatct atatggcaga ataaatcgtc catatattta tcaattaata
     9301 atgtctgttt ttcttcctag cagcttctga tttcttttta cgtcttacac ttggtttttc
     9361 ataatgttct cttttacgaa tctcttgcat gataccggct ttagcacagt ttcttttaaa
     9421 acgacgtaaa gcgctatcca aggattcatt ttccctaaca gtaacctctg acattaattc
     9481 ccctccctcc ggctgcgagg ttgtgcttta gcatatatac atacggagaa aagattaaag
     9541 cactgtacca attatacaca atccataagc tttcgtcaat aactaataag aattataaat
     9601 gtaaaaatct ccttctaatt ttctctcttt atcgcctaat aaatgcttta aggtatattc
     9661 tttggtctct tttaatcttt ttatttcaaa atctagtaaa tgtttcttta attcttcctc
     9721 tggtgccgta cagcttacga ctcttattat tcctcttact ttaactgtac tatactgaac
     9781 ccaggtaggc ataaaagaaa tgttttttat tcttgccttt tcattcccag acttttcaaa
     9841 atcaatattt aaaattattc cagcatctct tggcgggtct ttagggcttc tttgggaaga
     9901 aatgaaattg cctaaagaat aaattacaaa tacctcttct tcactgccgt cagctcttgt
     9961 aatggtcctg gtttccattg gctgaatgac gtgaggatgg ctgcctaaaa taatatctgc
    10021 tccggcatta aataattcat ccacaatata tttttgctct ttgtttgggt atctttcata
    10081 ttcattccca aaatgaagaa aaacaacgat aaaatcagga gataattctt cggctttttc
    10141 tatatctttt ttcatttctt ccagatcaat taaatgaatc aaataaggct tatcttttgg
    10201 tacagatata ccgttggtcc cataagtata agacaaaaag gcaaaggaaa tattatttat
    10261 gattttaatt ttaatcgtct cacttgcttc ctgacttgca taagttccaa tatgttcaat
    10321 accaacctga tcaagcacct ccagagtcct tataaccccc tctgatgaat tgtccataga
    10381 atgattattc gctgtcgtaa gaagatcaaa accagcatct tttaagcttt gagccaggga
    10441 atccggcgta ttaaactggg gatagcctgt atatgctctt tggggtccgc caaaagtaga
    10501 ttctaaattg ccaatggtta tatctgcctc agataaatac tgctttatta attcaaattg
    10561 cttatcaaaa tgataagtct ggttattttt atcataagcc acatccagtt gttcatcatg
    10621 ggtcataata tcgcctacga ctctcatctt tactgtataa agctgttttt cttcttcaat
    10681 aacttcctct tcattaaaat tagctgccat gacaagcttt tcgtcattcg tttcttttaa
    10741 atttattttt atattacaag ccgtcagaca aaaacaaaaa atcacacaaa ataccagcag
    10801 gggtcttttc attggcgcac agctccattc ttttttgact tttcaattcc ctaattcaat
    10861 tataaccaat aattgtcatc ttttcaaaga aaaaacggca ttaaatgccg tttagttcag
    10921 tttaatttaa ctgttttttt aatatttctt ctgtacgatt gagggcttca tcaatcttag
    10981 aaagatcttt tccaccagcc tgagccatgt taggacggcc acctccactg cctccggcaa
    11041 tctgagcaat ttctctgatt aaatttcctg catgaacacc tttcttaact aaatcttctg
    11101 ttgccattac cacaaaactt actttactat ctttaccaga agccagtaca atcaggccag
    11161 agcccatttt atttttcaaa ttgtctccca tatttctaag cgctgccatg tctaaatcat
    11221 ccagtcttgc agtaattaaa ggaatacctt caacatcttt tttcctggat aaaatatcac
    11281 caaccaattc tccagccgtt tttgctctta atttttcaag ttctttttga ttttctttga
    11341 tttcatttaa taaatcttta atgcgtcttg aaatttgatt tggatttgtt tttaataaac
    11401 ttgcagcttc atgaagaagc gcttcctgct gcctgtaata attaagagca gcttgtcctg
    11461 ttaacgcttc tattcttctt actcctgctg caacgccagt ctcactgata atcttaaata
    11521 atcctatttg tgcggtattc attaaatggg ttccaccaca taattccata ctaaagtcac
    11581 ccatggtaac aacccggact tctttgccat atttttctcc aaacaaggcc atagcaccca
    11641 tttttcttgc ctcatcaata cttgtttcct gaataacaat tggtaagttt tcaagtatct
    11701 ttttattgac catttcttca accttcataa tttcgtcagc agacaaagct gaaaaatgca
    11761 caaagtcaaa acggagcctt tcaggtgaaa catgagaacc tgcctgctca acatgatttc
    11821 caataagttc tcttaaggcc ttatgaagta agtgggtcgc actgtgattt ctggccgttg
    11881 ccagtctgtt ttccttatca acttctaaaa ctgccttttc acctgttgaa actgtgcctt
    11941 ctataacttt accaatatga gcaaagttat cccctgtaac cttaatacaa tcatatattt
    12001 ctatcagtcc attattgctt ttaatgattc ctttatcacc aacttgtcca ccactttcag
    12061 cataaaaagg tgtaatatcc acaattacag atacttctgc tcccttttct gccttttcca
    12121 caaattggtt gtccacaata attgcacgaa taatgacatc agatacggtt aaatcggtat
    12181 aaccaacaaa ttttgtcttc cattctttat taagttctgt ataaatcgtc gcttctgctc
    12241 ccatataact ggtttcttct cttgcttgtc gggctctttc tctttgaatt tccatttctt
    12301 tcttaaagcc ttcttcgtcc aaacttaagc cttcttcagc aagaatttcc atcgtcaaat
    12361 caattgggaa accataggta tcgtataatt taaaggaatc tttacctgaa agaactttat
    12421 tattggattt tttcaattca gcaataaaat ctttcagtat atccagaccc tggtcaatgg
    12481 tttcattaaa tctttcttct tcaacggaaa gaacctttaa gatatattct ttcttttcaa
    12541 ctaattccgg atatgcttcc tttgaagttt caataacagc cactgcaatt tcttctaaga
    12601 aaggcttatt aatacctaat aacttaccat gacgtacagc ccttcttaat aatcttctga
    12661 agacataacc tcttccttca ttagatggaa taatgccatc agaagccata aaagtaactg
    12721 ctcttatatg gtctgtaatg acacggatag aaatatcttt tttagcatct gtttgatatt
    12781 ttacttttgc aatttcacat actttatccc tgatatgttt tactgtatcc acatcaaaaa
    12841 tagatgtaac gccttgcatt actgctgcaa ctctttccag acctgcaccc gtatcaatat
    12901 ttggattggg aagaggatta taattacctt cttcatcctt atcaaactgg gtaaatacta
    12961 aattccatat ttctacaaaa cggtcacaat cgcagccagg agcacaatca ggtctgccgc
    13021 agccaaattc ttctccccgg tcaaaatata tttccgaaca cggtccacag ggacctacac
    13081 caatttccca aaagttatct tcttttccaa gtctgacaat tcgttcagca gataaaccga
    13141 cgcttttatg ccatatatca taggcttcat catcttcttc ataaactgaa acatagagtc
    13201 tttcttcagg aatttctaaa acttgcgtta caaattccca tgcccaggga atcacttctt
    13261 ttttaaaata atccccaaat gaaaagttcc ctaacatttc aaaaaaggtt gcatgtctgg
    13321 aagtcttacc gacattttcg atatcaccgg tacgaataca tttttggcaa gttgtcactc
    13381 tttttttagg tggtttttcc tgcccagtaa aataagcttt taaaggtgcc ataccggcat
    13441 taattaataa taaactttta tcgttttgag gaattaaaga aaaactttcc attcttaaat
    13501 gatctttact ttcgaagaaa gataagaatt tttcccttat ctcgtttacg cccaagaact
    13561 tcatttttct ttcaccagat tcctttctaa tgtatattta aagttttaaa gtaataatgt
    13621 attcttcttc acctatttca gaaacattca ttactttttc caaactaaat tcttcactga
    13681 gtagtatatc atgattttcc ttttgaacaa agcctattaa atatttattt ccaaaataat
    13741 cttcaaaaat aaccattttc tcttctttaa catgctcaat atattcttca agacataaat
    13801 gattaagggt catatattgt gcatggggaa tccccacata acgaaaatgc cagggttctg
    13861 aaataattcc cgtaatttct tttttttcag gcaaatagcg cagtacaaaa ccatacttcc
    13921 agctattatt atatagccac tccccctgag gggtattttt aaattcttct actaaagggt
    13981 ctgctgtttt taataacttt gcagaagaaa aatctattgc cagaccagtt tgatgctcac
    14041 tggtccccgg aattgccaca atctcagaag ccttttcttt ggcagtttcc aaatcatatt
    14101 gactggaaaa agcattgact ttagcattat aaagctgact ttgataacta taaggtctgt
    14161 atccgctaac cacaaagaga ccttctatcc cttcattttt tgctccatta aacatcgtaa
    14221 ttaaggcttc atatgcctct ttttgtaaca acatctgttc cttggttgaa ggaacaatgc
    14281 ctgcagcatc ctttatatcc gttggttgat aattctcaga caaaagattc catttattga
    14341 ctaatattaa actatgatcc ttttgacgcc taaaataatc aaaaacagct tccaaatcgc
    14401 tatcttcaat gattttagtt ttcctgtcca ctacctgctc tccttctgtt tcattatttc
    14461 cttctgtttt tgatatcttt cctgtactgt cttcttcatg atacacattt cttgctgaga
    14521 tttcattcaa taatttatat gaatacatgc ttaataaaat aacagaaagc gagagaaaaa
    14581 caaaagctat ttttttcatt ctatttcccc ctaaaaacat ttaaaaaacc tcatcccaag
    14641 ggattgaggt tttctcttta tatataacca tgtaaaaatt ataagaaatc aaaagaactt
    14701 tgtcaacata tccactttat taatctaaat ccgtcggctt gtaatttata gaagtctttt
    14761 ttgagaataa aaaaaggatg tgattaacac atcctgaatt ttaaaattac cttaattcct
    14821 caaaaatctc tccggctctt tcaatgagtt ttcttccgct cttcatcatt ttttttcttt
    14881 ctcttctgct tgaattcatc attttaaatc ccatcgcacc cgctatgcca cctattatca
    14941 tgcctgcagt gaatttatta ataatcatta ttgtaatctc ctttcataaa atgtataatg
    15001 tggatagaat atgaataagt ttgtagaatc ttgcattatt attatatatc attaatgatt
    15061 atttattcat actatagaac aaccatttgg aggtcatctg atattatgaa aaataaattt
    15121 tttacctttt tattagctgt tgttttaact tgtttttatt gctcgccagt gtatggagta
    15181 aatgcttcag caacaaatat tgtcaatcct tatcaggcga ttagctattc ccaaatgatg
    15241 gaatatctaa agcgattaga aaatcaattt gaagatttaa tcgaaatgga aataattgga
    15301 aaatcactgg ataacagaga tattgttctc gtaaaattag ggaaaggcga taccctgatt
    15361 catattaatg gcagcatgca tgccagagaa agaattacca ccaatatcat tttgaaaaat
    15421 attgaagatt attgtaatgc ttatagaaat aacagcagca tcagtggcta caatgtaaaa
    15481 gaattattag accaggtaac catttattat gttcctatgg tcaatcctga cggtgtcgat
    15541 tacacaatat caggaaaagc cagtatcagg gacccactat taaaaagtaa tattgattcc
    15601 atttaccatt ctccccaaac aggctggaat catacttcaa accgatggaa agcgaatata
    15661 aggggggttg atttaaacag gcaatggaat tccggttgga acaaaaacct taaatacgat
    15721 ccgggcaaac ccgcagatgc atattataag ggacctgaac ctttaagcga acctgaggca
    15781 caagccattt atcagctatc cattgaaaat ccttttatga ttcatgtagc ataccatacc
    15841 cagggaaaaa ttttttactg gtataaaaac caaacgggga cgctttatga acaatctctg
    15901 aatatctcaa aaaagattgc aaacttaaca ggatttaaag catatccttc gctaaattat
    15961 aaaggttatt ttgaaagtta tgcaggctat accgactgga ccatagaaga attaaagaaa
    16021 cccggcttta ccatggaatt tgctacaaat ccttattcag aaaagaattt cgatgaaata
    16081 tataaaccgg ccaaagcttt aggcctgctt tttgcccgcg aggcccttaa tttaaaatct
    16141 gtatataaca cagaagtata cataaaccag aaattaacac aagtttttga aaatgatgcc
    16201 aatgccagtg cctttattac caagtatgtc actaaagatt ctgacataaa aatcattaaa
    16261 aatggagaag tcttatatgc ttatgaacct tattcccctg aaaaattact gattatggct
    16321 aatgattttg caaatcttat atataattat gattatagaa acaacaacga agactggccg
    16381 gccttattag ctcaatatat tccggataca ggctttagta tgaaccatgt cattcttact
    16441 tctgaggaat taaatgacat gagaaaagaa gaaaaattac aaatcacagg aagctttcgt
    16501 tctgatattc attccattac caaaggggat agtaacattt acaccataaa aggtcaggtt
    16561 gattatcgct atttatctga cgatagtgaa gatagccttt ctaaaaatat tatgatttcc
    16621 atttatatcg ataatcattc caaagcccaa atcatctctt taatagaaga aaatatagaa
    16681 taaaaaaagc taggaagtcc tagctttttt aaattactat tcaagagaat gaataaaaat
    16741 accacttcta agttttggtt caaaccaggt cgatttaggc ggcataattt taccagcgtc
    16801 ggctatgtcc attaaatctt caatactggt aggatacatg gaaaaagcaa gcttcatatc
    16861 tgtatcagct cttctttcca attcttttaa tccccgaatg cctcctataa aatctattct
    16921 gttatcggtt cttggatctt ttattccaag aacaggcgct agtaaattgt cctgcaaaat
    16981 ggatacatcc agcctttcca cagggtcttc ctgattaaag ctgccttctt ttgctatcag
    17041 tcgataccag cttttattga tatacatgcc aaattcatgc tttgcttttg gtttgtaagc
    17101 ctgattgtct tctatttttt caacgataaa cttttcacgg atacggtcaa agaattcttc
    17161 ttcgctaagg ccgtttaaat cttttaccac tctgttgtat tccatgatat gaagttgctc
    17221 atccgggaat aaaacagcca aaaagaaatt aaactcttct tctcctgtaa agctgccagc
    17281 ttcttttctt ctttttaagc ctacttttac tgctgaagcc gcacgatgat gaccgtccgc
    17341 aatatataag caattaattt cactaaagta atgactgata gcttctatta ctttggggtc
    17401 atcaataacc caaaccatct gtctgacctg ctcttctgct gaaaaatcat aaaccgcttc
    17461 atgattgttc atccagttat ctaccagctg attaatttct ttttttgaac ggtaagttaa
    17521 aaagatagga cctgtattcg cattacaata gtccacatga ttaatgcggt cctgctcttt
    17581 atcttccctg gtcaattcat gcttttttat aacattattt acataatcgt ctattgaagt
    17641 acatccgaca attccaatct gtgccctgcc ttccataact aatttataga tataatagca
    17701 aggcttctcg tccataagat aaatgccttc ctgaatcatt ttattcaaat tgtcctttgc
    17761 cttttcatag acccttttgt catataaatc aatagaagga tctaaatcaa tctctgcttt
    17821 atcaatatgt aaaaaagaat agggattacc aataaccatt tttctggctt catcactgct
    17881 catgacatca taaggcaaag ctgctacttt atgaactaaa tcttctttag ggcgaaatgc
    17941 tttaaaaggt ctaagaactg ccatctcttt cctccttatt atttaaaaac ttccgcctta
    18001 ggcggaagtt ttttgataat taaactctat cctttggagt gtactttgtg tgaagaagat
    18061 gatgtgctct ttcactcatc ggatgtccta aataagtttc atacaattct ttgataacag
    18121 ggttttcatg ggattttctg ataggttttc cggcatcttc acggtaaatt gcctctgcac
    18181 gttttttaat gatttccata tttccgtgat gataaggctg tccaccacca ccaatacatc
    18241 ctccaggaca agccataatt tcgattgcat gaagatttcg tggatttccg gattttactt
    18301 cttctaaaag ttttcgtgca tttcctaaac catgagcaat accaatatta aggtctaaat
    18361 ttcccatttt aaccgttgct acgcgtacac cttccatacc tcttaattgt tgaaaatcaa
    18421 ctttttcaag ggtttctcct gtatataatt cataagctgt tctggttgct gcttcaataa
    18481 caccaccagt cgtaccaaaa atgactgccg caccagttga ttcacctaat ggtaaatcaa
    18541 attcttcatc ttctaattca ttaaaattta tattcgcttt cttaattaaa tgagccaatt
    18601 ctcttgtaga aattgaaata tcaacatcag gatttccatc cactgagaat tcttctctgg
    18661 aagcttcata ttttttagcc agacaaggca ttactgaaac gacaaccatt ttttctctgg
    18721 ggatgcctaa tttttcagca aaataagatt tagcaataga accaaacatc tgttgtggag
    18781 acttcgcact ggaaggtacg tctaataagt cctggaattg gctttcaatg aagtttaccc
    18841 agccaggaca acaagatgtc aggattggaa gttttacagt cttgtctcca tctaaatatc
    18901 tcttaatacg gtctaataat tctgctccct cttccataat ggttaagtct gctgcaaaat
    18961 cggtatcaaa tacataatca aaacctaatc ttctaagagc agctaccatt ctacctgtta
    19021 ctaaggttcc tggttcaaga ccaaattctt ctcctaatgc tgctcttaca gcaggtgctg
    19081 tttgcacaat aacaacctta tctggatcag ctaaagcctg cacaacagac caggtcatat
    19141 tttcttcatg aagtgctcca acaggacaaa cagcaacaca ttgtccacaa tgagtacata
    19201 cagaatcttc taaattcact tcaaaggctg gagccactac agcattaaat cctctgttaa
    19261 tacctgataa agctccaacg gtttgaacat cattacacat ggtctcacag cgtctgcaca
    19321 taatacattt atccatatca cggataatgg ttttagaata atcttttcta taagtggatt
    19381 gtgcgccttc aaaccttact tttcttaatc caaatctttc agctaaatct tgtaattcgc
    19441 agttaccaga ttttgagcac actaaacaat cttttggatg gtcagacagt aacaattcta
    19501 atacgattct tctggcattt aatacacgga tagtattggt tcttacgacc atgccatcaa
    19561 atacgggtgt tgcacaggca ggagctaaat ttcttctgcc ttctacttca accacgcaaa
    19621 ttctacaaga agctgcttta ttaaccatct ttatatcatg taaatcaaga tggcataaag
    19681 taggtatatc tatcccaatt ttgtgagctg cttgtaaaac cgtacttccc ttaggaactt
    19741 caacctcttt accatcaatt gtaactttaa catctgacat tggttttaca ctcctttact
    19801 attatttctt gatgattgca ctaaacttac atttttcgta gcaagcacca catttaatac
    19861 atttatcctg atcaatgaca tgcgttgatt tcactttacc agaaattgct ccaaccggac
    19921 atactctgga acaagctgta catccgatac atttttcttc aagaataaaa tattgaagca
    19981 gagctgaaca ttgtccggca ggacatcggt gttctttaac atgggcaaca tattcatccc
    20041 agaatgtatc tatggttgaa agaacaggat taggtgcagt ttgtcctaaa ccacacaatg
    20101 cggtatcttt aatggtttca cttaaagttt tcaacatttc aagatcttcc atggttccct
    20161 gacctttagt aatcttatct aataactcag acaagcgttt tgtaccaatt ctacatggcg
    20221 tacatttacc acaggattca tcttcggtaa attctagata gaattttgca acgctgggca
    20281 tacaatcatc ttcatccata acaatcatac caccagaacc catcatggaa cctttagcaa
    20341 ctaagttatc aaaatcaata ggtgtatcta aatctttttc agtcagacag ccgcctgaag
    20401 gtcctccggt ttgaacggcc ttaaatttct tactgccttt aataccgcca ccgatatcaa
    20461 atataacttc tcttaaagtt gtacccattg gaacttcgat aagaccaaca ttattaatct
    20521 taccagccaa agcaaatact ttcgtacctt ttgatttttc agtaccaata gatgagaacc
    20581 aatctgctcc ctttaataga attacaggaa tatttgcaaa ggtttcaacg ttatttacat
    20641 tagtaggttt accccaataa ccagattctg cagggaaagg tggttttgtt gtaggttcgc
    20701 ctctttcacc ttccatagaa tgaataagag ctgtttcttc accgcagaca aaggcacctg
    20761 ctccgtactt aatttctata tcaaaatcaa aaccagtatc taaaatattc ttaccaagta
    20821 aaccgtattc tcttgcatct tcaattgctt ttttaagacg ttttacagcc agaggatatt
    20881 ccgcacggat atatactaaa cctttattgg caccaatagc ataaccgcaa attgccatag
    20941 cttcaacaat agagtgagga tctccttcta agatggaacg gtccataaat gcaccggggt
    21001 ctccctcgtc ggcattacaa accacatatt tttgatcgct tttgttttta taagcaaact
    21061 cccatttaag acctgttggg aagccaccgc ctccacggcc tcttaaacca gactttttaa
    21121 cttcttcaat tacttgttcc ggagtcattt ctgttaaagc ttttccaaga gcctgataac
    21181 cgtctcttgc aatgtattct tcaatattat caggatcaat aaaaccacag tttctaagcg
    21241 ctacacgcat ttgcttttta tagaaatcca tatgcttaga atcatcaata gctgtctgtg
    21301 tattaggctc tttatataat aatctttcaa cttttcttcc tttaatcaag tgttctttta
    21361 caattatttc tgcatcttca ggcttaactt gtacatagaa ggtgttgtca ggaagaattt
    21421 ttacaatagg tcctttttca caaaagccga aacaacctgt ttttaaaacc tgtacttcgt
    21481 cttcaatacc attttctttt aaatacttct ttaaattttc aacaatctgt atagaattag
    21541 aagaaacaca gcctgttcct ccgcatacta acacatgcat tttatatctg gacattccga
    21601 aaagcctcct tttacaatta atcgtctacc gttttatgtg tgactggtat aatgccatct
    21661 aataattccc catgaatgat atatttatct actatttctt ttaccctgtc tttatttact
    21721 tcaccaaata caactggttc ttttccagga atggttactt ctactgttgg ctctgcataa
    21781 caatagccca tgcagccggt ttgagatacc acaacattat ctaactttct gtttgctact
    21841 tcatcaataa aagcatccat aacgtctcta gctcctgacg caataccgca agttgccata
    21901 gctacacgga ccagaatcat tttttccatt tggtcgcctt tttctcttat gtctacttta
    21961 gatttatatt gttcccgcaa ttgttttaat tcttctaaag acttaatctt acccatgctt
    22021 ctcctcctat aatttattat tcatattctt ttagaatgga tttaacctga tcaggagtaa
    22081 cccggccata tactttatca ccaactaaaa caactggtgc taatccgcaa gctcctacac
    22141 agcgcaatgc atcaagagaa aacttaccgt ctggtgttgt ctcaccaact tcaatattga
    22201 gcgttctttt tatttcatct aatacctttt ctgctcctct aacgtaacat gctgtaccca
    22261 tacaaacaga aatgggatgt tctcctttag gaaccattgt aaagaatgaa taaaaactta
    22321 caataccata aacttttgat actggaattt ctaatttttt agcaacaaat tgctgcactt
    22381 cccttggaag atacccaaat atttcttgag ctttgtgtaa tacagaaata acagctccgt
    22441 ttttgtcttc taaaccatca ataaatgctt ctaattcacg aaaacagctc tctgacagct
    22501 tatttttgca agtttcgcaa ttagccattt cccttcccct ttcatataat tgtataatta
    22561 ataatataag aatcaaactt cgaaatcatt ttgatacgaa gtgattttta aacaagttta
    22621 atgtatacaa tataacttaa aaataacata gatataatca cattgtcaat ataataacat
    22681 gaataataat ttttttccat tatctcttat tcaggtactc aataactttg gcaggcgatt
    22741 tttccgctaa ttcaataaaa tgctctcttt ctgatatatc ttgtaaataa tgtgcatctg
    22801 aagattgaat caaattaaaa gtcgagtatt ctgatatata tttgtcaaat tcagcatatc
    22861 tggaaatttc gatggtctta acccctaaat cttcaggtat ccagcccaaa ttggatataa
    22921 tgctgtaaga ttttctgtca atatgtgcag gatagaccac tccactttgg cgttccactt
    22981 cttttaccaa gtcatagaca cttaatgaag tagcggtcag aagtaacctg tcattttctc
    23041 caatcacgtc gtcatattcg ttaagaataa gctgttttcc aaagatgtct gtcctgtttt
    23101 ttaaaggagg caggctgttg tatacgattt cctgcattgc ctttgctgcc tcaagacttg
    23161 ggaataaaca tatcatatgt acttcttcac tgctttctat ctccatacca ggtattacta
    23221 aaagattggt accctgggct accttcatga ctgcttcaac attttcacaa ctattatgat
    23281 ctgtaattgc aatcatgtcc aattctttta ataaggccat attaacaata ttattaggcg
    23341 tcatatcttc atccccacat ggagaaagag ctgtatgaat atgaaagtca taataatatt
    23401 tcatggcttg tcctcttatt ctattcctaa atgaatcaat tctttaatta aacaataagc
    23461 atccttattg gttgttaaaa taggaatttc ttcattatta gctttttcaa cagtttcttc
    23521 atccactttt gccccttcag ccacaataat gcaggataaa tttaataaag aagcaactgc
    23581 cacaatattc acatgacttt gtatcgtaat ccagatggaa gatgcttttg catgggccat
    23641 cacccagcta agcaaatctc ccacataaac cgtcgatatt tcattttcta aggctgtttc
    23701 ccccgccaca acagacagat ctagtttttc acagatatct tttactttca tccaatactc
    23761 ctctttctaa aaatccttat tatttaatga agctggtatc ttctgagcta aagccaccat
    23821 atcctcagcc atttccctga ctttttttct taacataaat atacaatctt caatattggc
    23881 attatttcta acaatatcct ctgccaatgc ccggcagttg ggcgccccgc aagaaccaca
    23941 atcaatctga ggaagttgtt catagattct ttctaattcc tccatttttt tgatagcaat
    24001 attcatatct tcgtccaaac tcatgacagg cttggagaga acttctttat tccagaataa
    24061 agtaatgtct tcagtatgta aattcgcaca atctcctgct ttttcttcca gagtattttt
    24121 caaagctttt ttaagcttgt ttttagcaac aaaactattt tcaacggtta atggtccgcc
    24181 cagacaaccg cctgtacatg ccagaccttc tataaaatta acattaacaa gcttgccatt
    24241 ttcaacttct tccaggactt taatcacatt ttcgatacca tcaacggcaa taaaattatc
    24301 aatttccagt cctttacctt ctccgccgga cattgcccat cttattccat cataagaact
    24361 tgttctaagg ttttcaatct tatccagtct ctttagggta cctgccagct tcttataaac
    24421 ttccttaatc gaaaggacct tatcgacatg cgatttttca atacctattg gatttctcgc
    24481 attcgttgct tttgcagcac atggactaat aaaaaacacc ccgatgtctt tttcatctat
    24541 tcctctttta attaaatcat tttttacaat tcgggcagcg acctccatag gagatatgac
    24601 tggaagaata tgttcaatta attcaggaaa tcttatctga attaagcgga tgatgacagg
    24661 gcaagccgag gatattacag gtttttttaa atttgaactc tttaataatt tttttgattc
    24721 ctccgtaaca atttctgcag ctcttgaaac ttcaaagacc tcatcaaagc ctagttgctt
    24781 taaccctgtt aaaatgacat tgacatcata aatatcttta aactgggcat ataaagtagg
    24841 cgctggaatg gccactttgt atttatattc agataaacag tccaacttat ccgtcatggc
    24901 ttttttggca cggtttctgc aaacttttat acaaagtccg caatcaatac atctttcatc
    24961 aataattttt gctttcccat gccttaccct tatggcttca gtaggacacc gtttaataca
    25021 atccgtacaa cctacacatt tatcttcctg cagcgttacg gaatgtaaat attctatcat
    25081 ttaatcacct tctctgttta aataaactat tatctccacc ttagttccct tccctagttc
    25141 agatgttatt ttcaaatcat cactgtatcg tttcatattt ggcagaccca tacctgcccc
    25201 aaaacctaat tctcttattt tatccgatgc cgtggaatat ccttcctgca tcgctaattc
    25261 aatatcttct attcccgggc cggtatcttg aaatacaata taaatttttt ccggtgagat
    25321 atcgacatcc accattcctc cattggcatg aagaaccata ttaatttctg cttcatacat
    25381 tgctatagca attcttcgga tgatatcaga agcaacccct aattgcttta ataccttttt
    25441 tacctgactg gatgcttctc ctgctaataa gaaatcatca ccatctactt caaaatttaa
    25501 cttcataccc ttcctcctgt tttttttcat cgcctgttaa tccgttgcta tataacaagc
    25561 cacatgctat ataaagagga tatttagtct ttaaaataac gatatttttc ttttctgcta
    25621 attgaatggt ttctggatct ggttgtttac cccgtacaaa aacaattgct ttaatatcca
    25681 tcatctcagc tgttctaatc acttgcggat tcgtcagtcc agtaagaagt aatacttttt
    25741 ctttaacaaa tgctaaaaca tcgctcatta aatctgagcc acaagctaac aaaacttctc
    25801 tgtccagacg gtcttctcct accaatacct ctgcatttaa tatttgcttt acttcctcta
    25861 atttcatata aacctcctac acgatttata aaatattgta aaaaaccaaa caaaacattt
    25921 tattgagatt tatctgcttt taatttagga aatttttttc tccccggaag tactatttta
    25981 aataaattgg tatttaaaaa gctatagacc ctaacctggc ctacattggc aaaaggcttc
    26041 tttattctgt ttatccctaa accataattt aaaaagcgct cttttttatc catcaaaagg
    26101 agcctttgat aaagccaagg attccttctt ggtgggtttt gatcctgcat aattttttta
    26161 atactgcccc tgtaccatat cgctccaggg tttgatatgg atacctgatc ttcttctatt
    26221 ttgacggtga tttccctgga catatcccaa taatccctat gaacgacagc attggacagg
    26281 gcatcaaaaa ccgattgaac cggataatca tagtctttaa aaatatcgga aatgattgct
    26341 tcggctttat ccagcatttc taaaatattc cctctaaccg gtattactct gccatcataa
    26401 tcaatcctga tacctgtatg aggcagatag ttctgaggat ttttaccaaa taataataaa
    26461 ccgcccattg taggatgata ttctgattta ttagaatccc gccctataat ccctaaacct
    26521 tccagataca aatccttatt tacttcaaat atattatcct ttcccaaaat atattgcttt
    26581 aacatttctt catccaattc gcttatgggc actttcctac agagcgtttg ttctgagctg
    26641 gtcaggccat tttcctgaag catattggca atttcatgcc tgcgggctac atccgttgta
    26701 gaacctcttc taatataaaa tgtaccggtt tgtcttacct gatgagggcg ctgattgctt
    26761 ttataaatag taatgactgc cacatttttc ccatgatagt caatgatatc aactttaatc
    26821 ggaacgggag gatcgcagcg gttgcaaata atttgctgga ttctttcttc atcaaaaggt
    26881 tctaaatcaa ctccgacaac ttccctggat ttgtccttta cgccaaaaac aatatgccct
    26941 cttccgccac tcgaattggc aattgcacat acatctttaa caaattcttt tttatcagaa
    27001 tctgtatcta accgtaagca ttctttaaaa tcgagcttaa atccctctgg ttgcttaagc
    27061 agcttctcca acttctcaat gtccatttcc ccacctcttg aatgtctttt atattttaat
    27121 aaaaaaatga caataaaggt cttattatat attgtatatt aaaatgagaa tgaataaaag
    27181 aataaaagaa aaaaagtaca gaaactctgt acttttattg aatatcaaat ttataaccaa
    27241 caccccatac ggtttttata ctccaggatt ctttttcatc gaatttttct cttatccttt
    27301 taatgtggac atctactgta cgggtatctc caacataatc ataaccccaa atacggtcaa
    27361 gcagctgttc cctggtaaat acctgattgg gattagaagc aaggaaataa agcaattcaa
    27421 gttcttttgg gggaagatcc atctgctttc catgataaat gaccgagtaa ttacctaaat
    27481 caatggttaa attaggtata acaatctttt tactgttatc ctctttaggt tcataacgtc
    27541 tcaatacggc tttaactctg gcaatcaact ctttaggctc aaaaggcttt acaatatagt
    27601 catcggcacc taattctaat cctaaaacct tatcaaaagt ttctccttta gccgttaaca
    27661 tcacaatagg aatattgctg atttttctta tctccttaca aacatcataa ccattcattt
    27721 ctgggagcat gatatccagc aaaacaagat taggtgcaaa agataaaaaa gctttaacgg
    27781 ctttgtcacc agagtatact tcttttgttt cataaccttc tttatttaaa tatagggaaa
    27841 tgagttctgc aatatgtaca tcatcatcta caataagtat tttttgcttg accgacatat
    27901 tataaccccc tgtagcaaga atgcaacccc tacaatccgt aggggccttc tttttatcaa
    27961 ttatttgaaa gttttgcttt aagtgcttct aattctttca aagctgcatc atcttgcatt
    28021 gaactttcaa gcttttcaaa ttcactttca agactgtcac cagtaacttc catcgccaat
    28081 gattcactgg ctaaagcttt atcttccatt gcttcaatct tagcttccat tctttcaaaa
    28141 gattcaaaag cactggtatc gtttaaatcc gcataagttt tattaacggt ttcctgagct
    28201 tttgctcttt gtgccctggc cagaagtaat tctctttttc tttttgcctc ttcaatctta
    28261 ttattgagct ctacaagaga attctttaat ttatctgtta tttccttttg tttatccaaa
    28321 tgaactttat attcttctgc caatttcgca tgctcatttt ttcttttaat ggcttttaaa
    28381 gccaattcat cattttgctg ctgaactgct aattctgcct tctttgccca ttcttcctgt
    28441 aattttaact gctcctcata ttgtctttct aatttcttct catcagccat tgcctgtgcc
    28501 acttgctttt tagcctcagt gtactgttcc tgcatgtcta aaataagctg atttaacatt
    28561 ttttccggat cttctgcttt gtcaataaga tcattaatgt tggatttaat taaagttacc
    28621 attctttcaa agatacccat ctcactcacc ccttagtctt attataatct attatatata
    28681 aagtatacta catattgcaa tatactttat atttaaaaaa taaccaaatt atattgtgtg
    28741 catctctaca atatgtatat aatagtactg ccttatatat tttaagtata tattctctct
    28801 attcttttgt caagaaaaaa tttaagcagc catttatggc tgcttaaaag cattcattat
    28861 tttaattcaa caatggttac tcctgtctct ccttctccat attttcctaa acggtatgat
    28921 tttacatgag gatgtcttct taataattga tgaattgcct gacgtaaagc acctgttcct
    28981 tttccatgaa taattgttac ttgtgacaga ctggataaaa aggcttcatc taaatattta
    29041 tccgtattga tgatggcttc atcaacggtt tgtcctctta aatccagttc cggaccaata
    29101 ttcattgtct tgctcttagc cgcagtagtc cttgttgctt taattttctt atcctggttt
    29161 tcttcttcat catctaaaat taaatcagac agatgaactg acagtttcat aatacctgcc
    29221 tggaccgtaa catcaccatt agcattggga ggaatgatta caattccctt ttgattaaga
    29281 ctcgtaacga atactttatc accttttttg agcttttcag gtgtcttacc tgtaatcttt
    29341 ttactgcctg gctttaagat ttcttcatcc agttctttga gcctttccct taaactacct
    29401 ctggcttctt ccatatccct atagtcaata actttttgag cttcccgggc agatttctga
    29461 atttccttaa tgattttatc ggcttcatct tttgcttctt gcaggatttt tcttgcttcc
    29521 tgtcttgctt catgaataat cttttctttt tgtttgtcca gtttttcttt ttgctcttca
    29581 acaattttct tcagggcttc tgcttccctg cggtattcct gggccctgtt ttgctcaaga
    29641 atagccgtct ttttactgat ttctaaatct gcaattaaat cttcaaattg cttatctttt
    29701 ttagcaataa ataaagctgc attatcaatg atatactcag gaagtcctaa gcgtttcgat
    29761 atggcaaagg cattactttt gcccggaatt cctataagca acctgtaggt tggtctcagg
    29821 gtttctatat caaattcaca ggaagcattt tcaatcccat ctgttgataa agcgtacacc
    29881 tttaattcac tgtaatgggt cgttgcagcc gtacggacct tcatctttaa taaatagtcc
    29941 aaaatagcca tagcaagggc cgctccttct gtgggatctg taccagcacc taattcatca
    30001 aaaagaacca gggaatctgg cgtaactttt tttagaatat ccacaatatt agtcatgtga
    30061 gcagaaaaag tacttaaatt ttgttcaata ctttgctcat ccccaatatc tgcaaaaaca
    30121 ttatcaaaga ctgccagctc tgaactgtca ttggcaggaa tatgaagacc tgcctgtccc
    30181 atgaggacaa ataaaccaag ggtctttaag gacacggttt taccaccagt attgggtcct
    30241 gtaatcagca aagttgtaaa ttgatcccct aaataaatgt caataggcac cactgtcttt
    30301 gggtccagca aaggatgtct tccctttttg atatggattc tgccttcatg attaaaacgg
    30361 ggctctaccc ccttcatttc cagtgaaagt tgtgccttgg caaaaataaa atcaagacga
    30421 atcagaagtt ctaaattatt ttttataatt tcgatgtttt gggcaaccat ttccgtcaat
    30481 tgccctaaaa ttttctctat ttcatttttt tcttttattt taagttccct cagtagatta
    30541 ttcatctgga caatgaccat aggttcaata aataaagtgg caccacttgc tgactggtca
    30601 tggacaatcc ctggaaaact attttggtat tcctgcttta caggtacaca ataacggtct
    30661 tctcttatgg ttatcacagc ttcctgaagt tttgcctgat tcgcagaaga ataaataatc
    30721 ttttgcaact gatttttaat cttttcattc atccctttaa tttcttttct tatattactt
    30781 aatgcaggac tggcatcatc agcaatttct tcttcagaaa gaatacacct gctaatttcc
    30841 cgctccaggg caggaagggt cgtaatccct tcaaaaagag catcgagact tccatagctt
    30901 tcttcttttt tatcatcttt ataataattt tttacctttt tacatacata aagaaaatca
    30961 gccatatgca ttaattcagc cgctgtaagg attcctccga tatccaccct ttttatagag
    31021 cttcttatat ctttaaaacc tcccagagga atcgttcctt tcttaatcat cattgtcagg
    31081 gcatagctgg tttctttttg tctttgaata atttcattat aatcatctgt aggtattaat
    31141 tctccggcta tttcttttgc catgggagaa acagcataat ttactaattt atcaataatc
    31201 ttatgatatt caagggtttt taaacttctt gattccataa agcaattcct ttctaaaatg
    31261 ataataaact accattgact aaaacttgtg ttaattatat cattaaatta aatgataaac
    31321 tacttttctt tttttagggg gacaagccaa tggcattcat taatccaaaa gatttatatt
    31381 atgaaatctt ttcatctata caatctaact ttagtatgcc tattaaaata tcgtcaaata
    31441 ctaaagtaaa ttccatgaac tttaaagtcg ttctggaaaa agaactcaat gcagcatttg
    31501 atcatcaaaa gctgtcttta gaagaaatca gtcagcgtat tgaaaatgcc attgctgaag
    31561 cctctaaaaa atacaattta agtcctgaat taataaaggc cgttataaaa caggaaagta
    31621 atttcaaccc caatgccctt tcttctgcag gggcacaagg actgatgcag ctaatgcctg
    31681 caacagctaa aatgcttggg gtagaaaatc catgggacat tgaagataat attgatggag
    31741 gttcccgctt tttaaaagac atgttagtcc gctttaatgg aaatctttcc ttagccctgg
    31801 ctgcctataa tgcgggtcct ggtaatgttg aaaaatataa tggaattcca ccttttacag
    31861 aaacccaaaa ttatgtccct aaagtattgg cctataaaaa acaatatgaa gcatttaaaa
    31921 aagagtgacc aaagtcactc ttttattatt ttcaattata attatattat ttcaatactc
    31981 tgactgcttt tacatacctt cttgcataat agtcttcact taaactgtct atggttactc
    32041 ctctggaaca ttctgaatga ataatgctgt tattaccaat atatatggct acatgggaaa
    32101 ttgctccgtt gtttggtcct gaagtatcaa aaaatactaa atctcctggc tgtaaatcac
    32161 tctttgccac ataatagcca tcatttgcct gtgaactgga acttctgcta agagaaatac
    32221 caaacttttt cataacggat tgggtaaaac ctgaacagtc tacacctctt gtcaaatccg
    32281 ttcccccata tctgtaaggg gttccaataa attgtttagc atatgtaata atttcatctg
    32341 catttttatt ataagacgtc actgcttctt cttttttatc tgctaaaaca tttttatcaa
    32401 gaacctttac ataatcacca aaaatataag cttcctgacc tttataatct attttatacc
    32461 aattgcctgt ttttcctgta acaactaaag gatcacttct gttaacttgt cctaaaactt
    32521 tgcagcttgt attaggctca actcttatat taaccctgtc tccagtggat attgcatcca
    32581 ttgacttaat atttaaatca tcttttaaaa cgtaaccttt actttcatca tccaataaaa
    32641 cgatgtaaca atctccttct gtacctacta tttctaactc ttcatcttta tcaaccgtaa
    32701 caataacttc ttttgtttca tctaacttat cccaaacttc tacgccatcc ttattaacca
    32761 gtgccagtgt ttgtccataa acatccgtca tcgttgctaa tgaaaaaacc ccacatgcaa
    32821 aagctaccgt tagaactctg cgtaagccta tcataaattt taacccccac tgagaatttt
    32881 gttacgtaat tgttacaagg ttattatata aatatttaaa gtatttgtca attactttta
    32941 aaaaatgtta tcttatttat aaattgcaat attaaatgcc gtgcaaaatt tggtaaatcc
    33001 attgcttact g
//
LOCUS       DAOSUD010000016        32315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_953_length_32315_cov_26.870769, whole genome shotgun sequence.
ACCESSION   DAOSUD010000016 DAOSUD010000000
VERSION     DAOSUD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 32315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32315
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_32315_cov_26.870769"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1715
                     /gene="glgP"
                     /locus_tag="PLG49_03660"
     CDS             90..1715
                     /gene="glgP"
                     /locus_tag="PLG49_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOQ16446.1"
                     /translation="MVNKKLPKVAYFCMEYGLDSSFKIYAGGLGILAGDYLKGAKDYN
                     YPIVGIGIKWKQGYTDQFIGEDGRPYDCYYNYQYDFLKDTGVTVSVKIRQRDVLCKVW
                     EVTGFDNAPLYLLDTDLPENEDAWITGQLYGWFGEERIAQEMVLGIGGIRALRALGIN
                     VDVYHFNEGHALFAGFELVREKMEAGMSFEEAVAASKDEIVFTTHTPIIQGNESHHLD
                     RIMYMGANNGLTLEQLVSLGGAPFNMTVAALRLSRISNAVSQLHCKTANKMWAHINNR
                     SEIIGITNGIHLPTWVDERMIECAEKGGDLWKLHMDNKKKLIEFVKERTNVELDMDKL
                     LIGFSRRAAPYKRSNLIFTDEKVIAPLLKERKIQIVFSGKAHPLDDTGKKNVENIVAM
                     TKKYPQSVVFLENYDMTIGAMLTKGADVWLNNPRRPKEASGTSGMKAAMNGVLNLSTL
                     DGWWPEACKHGVNGWQFGDGFESEDEKELDAHDLKALYKVLTEEVIPTYYDRRDKWVE
                     MMKDSILSTKDTFSVKRMLEEYYEKLYIKQEEK"
     assembly_gap    1828..1927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2122..2772)
                     /gene="pgmB"
                     /locus_tag="PLG49_03665"
     CDS             complement(2122..2772)
                     /gene="pgmB"
                     /locus_tag="PLG49_03665"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOQ16447.1"
                     /translation="MLKGVIFDLDGVITNTAEYHYQAWAYLGKEIGIEIDRTFNEKLK
                     GISRMESLELILEHGNKADLYTKEEKMALAEKKNNYYKESIKQMTKKDLLPGIEKLLQ
                     ELKDHQIKIALASASKNGPMILKKLEIEAYFDYIVNPAHIKNGKPAPDIFLDALNGLG
                     LKAQEAIAIEDARAGVEAIQKAGMIAVGVGVEADVMVNSTEELSYDFLVQVLASFN"
     gene            complement(2787..5078)
                     /locus_tag="PLG49_03670"
     CDS             complement(2787..5078)
                     /locus_tag="PLG49_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HOQ16448.1"
                     /translation="MNKKTIFGVDEWKIIESNLHKENFRLAESVMSLGNGYMGMRGNF
                     EEKYSGDMLRGTYIGGVWYPDPTRVGWWKNGYPKYYGKVINSTNFIGIDLLLDGEELD
                     LFKVPLEAYYRELDMKNGILYRTFISNINGKRTKFEVTRFLSIVQKELCVISYEATPL
                     NYSGEITFIPYLDGDVINEDSNYNEKFWLETDKGVSVKEGFLIMKTKENPFGVPRFAV
                     CNRMKIESSKDCAIEIIEKDKYVANKMTFRAKENETVRIIKYISVVTSRDYKEEELAH
                     KSLKLIGQAEKKGFSSLKKEQMEAWQRRWEKADVEIKGDTADQQGIRFNLFQMFSTYY
                     GEDPRLNIGPKGFTGEKYGGATYWDTEAFVFPVYLSVADKEVARNLLLYRYNQLEEAY
                     YNARQLGLKGALYPMVTFNGIECHNEWEITFEEIHRNAAIAYAIYNYVNYTGDKTYLE
                     DYGIDVLIGISRFWADRVHYHKKKDVYMIHGVTGPNEYENNVNNNWYTNTMAAWTLEY
                     TLEVIDYLRKIKKDHRVEFHQLSQEEEDKWRDIIKKMYYPYDKERGIFVQHDTFLDKE
                     LMTVEDLLEGDRPLNQNWSWDKILRSCFIKQADVLQGLYLLNHKFTFVEKKRNFEFYE
                     PMTVHESSLSPCIHCILAAELKMEEKAVEMYKRTARLDLDNYNNDTEDGLHITSMAGS
                     WLSIVHGFAGLRTFGEKLAFSPFIPKDWERYSFKINYRDRLIKVDVNRESVTITLEKG
                     DPLKLWLYNIEYDLIYKISCKLI"
     gene            complement(5132..6001)
                     /locus_tag="PLG49_03675"
     CDS             complement(5132..6001)
                     /locus_tag="PLG49_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOQ16449.1"
                     /translation="MEKTLLAAIQNGNLGIAEMLIEKGLNLDYRDEKGNTALHYASEN
                     GCKDIVLLLLNKGANKDLVNNDKETALFLACQSGQKETAKLLIQQGANVNISNNDGIS
                     PFHYACMNGQKELAELFILNGVDINYTNNYGETALHLACNNNRKEIIKILLSNGIDIN
                     HTTNKGYTALFYACKNTQPESVQLLIDAGANIYATDDFGRTILHEACRNNKKEMVKLF
                     IEKGLDINARSKTGDTALHEACMYGHKEIVELLLTYDHLNAKIENSYGKTPIQYASEK
                     GFMEIVERLLPVS"
     gene            complement(6018..9317)
                     /locus_tag="PLG49_03680"
     CDS             complement(6018..9317)
                     /locus_tag="PLG49_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOQ16450.1"
                     /translation="MNNNFLNLCEAGKLEEIKQYLSEKEDITVKDMFGRNALHIAALK
                     GYTEIVQLLIDAGVPADEPDKDGETPLYKAGQNGFLDIAEIFIKNGANIYIKNKNNES
                     LLHQAANIKDLLIAELFLKNGIDVNAVDKNGKTALHQACLKRDKDLILLLLQYGADVN
                     IKDYQGRTALQETFRYKDIEMIELLIEHGADIHFTDPSGNSFLHLACQEDRKEIAEIL
                     IEKGINIDEMNNQGMTALHLACYLGKIEIGKLLIHKGADVNLSDNKGNTALHYAMTRG
                     NKEIIQLLMDKGADINAKNNDGTTVLKAAYNSRQYDIAEMLLEAGLMDDFNNDIGAWI
                     FHTACSKGHQKIVEALYHQGIDINITDSFGDIPLTSACLHGQKEIIDFLISHGADINY
                     VNKMGTPLIFMLYERRKRDIFEYLLNFNANVNLKKASDQRTVLFLACQNEDTEMVQLL
                     IHHNADINVKDRRNITPLYLACETGNKEIAELLLEVHADVNVKTLGNEKSILHLAVEK
                     QNKDIVELLLQYAKELNPQDMNRNTPLIYACNDGSKDIVELLLSYGANANEKNNQGET
                     ALHIAIKQGNKDIADILMSYGTDVNITNNSGDTALHLACQKGDKDLVLLLLSRGADIE
                     NKNHFSESPLFTAVKNRNHDIAQLLILNGADIHSRNRDGESLLEIACKNGSKDIVRLL
                     IEAGADIHAKNANGETALGVACRYSVKEVIRLLIEAGADINEKNANDESLLYRACRMS
                     NKELVEVLLSNGADVNARCRDKTSIFEACWDARKEIVQMLIQYGADLNVKDDNSKTVL
                     HHACNQEEILELLLTSGAEVNATDDRNQTALHLAAKGNNPKIVKLLLEFGADNSIKDE
                     DGCTAFYIAALYGKTGNIESFLNFNHPFNVNEQNFGGNAPLHYACLLGLTECIELILH
                     RGNGDVNITNAEGRTPLFNVCLESGHYYFGPYCLHRNVNHEQNKKNAEILIYSGANVN
                     ISDNRGLTPLMLACRSRNEKLVESLLNAEADIEMTDNHGNTALMFACDNNDPAIVELL
                     LSRAANVNSTNHDGISPLHFACREGNRNIVRMLLQKGANPHVKNSKGQTPLDIAVEHN
                     YNALVKLLLEY"
     gene            complement(9354..10022)
                     /locus_tag="PLG49_03685"
     CDS             complement(9354..10022)
                     /locus_tag="PLG49_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HOQ16451.1"
                     /translation="MYRYNPDRLTQTQLWLLLLSGVLTEQNEVRHDTLYGYEATKEHI
                     YLVKQALERDWGIRNREDLYSSLNWLLHSGHRTDFNRNFRYISLLPYSLEEKELNRIP
                     SGTSLYNSYQLSKLNKYILPRAGIAAWDFGRYITLCRDGALIKYISDKEAWELMAKIG
                     PMAQQSYSSWYEYGLAYMVGRLAWLDKLTEENVKWHVDLLRRLLFKEDSPWLKIPWDI
                     ALDI"
     gene            complement(10071..13373)
                     /locus_tag="PLG49_03690"
     CDS             complement(10071..13373)
                     /locus_tag="PLG49_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ16452.1"
                     /translation="MDKVKEIYENYSLRIDINQWEKLLNNENYWRLDVKQILFTELFG
                     FLLNHYHLPHKIWLLLDECFLWTKQREHLYKKFGDHVDFLLDEIDNTVEFNYNFPVDK
                     PFDYDSFLLYREQAFWALFHGNFELAKESIDQAQAIYSEDYDLIRIRGFYYCETGDYQ
                     KALKEFNDLLEIKRDDIDAYFRRADLYIKLRQYKDALKDYQWLLERIPQDPNALSGLA
                     RCYMALGNLLEAKTVFEYIIELYPCDIHAYLQIYSIDQQLINHYRLKLQEEAEQDSLR
                     ITIAQLLYELGWYDKCFEEIQKMLNSPKVNSSLYLLAGKALCRLEKYEEGLVWLEKAL
                     DLAYINKENGYEILVERGNCYFAMDHLDKAISDFLTAVNINPYDAELLYQLADALRIK
                     GMYKESLEKVNRAIGLNPDKWRYHALKGVIHFYNFDYEKARDELQMIVEQNPEHIQMW
                     FCLAYSYLKSGDYQKAKESFKVAIDWAVGFDDLIWFYLFRAENYITMQNYYSAQKDIE
                     TYKKAKANSFAGYLMSGCLYYLMNNKSKAITEFCTASDRLQTSFSLAKAAFCACYEKG
                     MPDKTLIYLKRMMNIDAADEWLQVMIAKVLMQLTSKKDAFLLIDDYCDSLKNQMSKDY
                     QDIYLFILLSHLKSLAEENPSRYDELVNYISGKEDISKLMFGDNKEIAMPVSKERKLF
                     SQFVQDKFEYKLEDICQLSLSKDNILGQLNWIEILIEMRFNETALCDLYLIIQYINHM
                     QEQKEIDSAIYIKLIRIFLCLVRFITVNYTKDNKALVDKALVVSDEMLACLEKKNNAH
                     FGIYAFRLSCAKNLLLCTIEDFHEWLRHSKELMQKYKDFKDYEIQKWFVINLLNLSRK
                     FKISRPEGDYHEIFKWVNEFSGEYLDSENLNFSEAVFNALVAIGSAELEMEKVDKDIL
                     IEICDKVLDFYKKRNDCPVISKVGLIACICNKAMILGSLGQFDKAKGVLDEAEEIARV
                     FGRSVGNMSYPAILCIRGATILKEFNQKFLSGHYAECNKKELKQALKYFEKALKFKES
                     YYACYAKECIPYTLFLMDKMNNKSNYKKIKKLLAEIQEDGEKKDKSAVNVFFSDFDFD
                     EEYKAVIKEAKQ"
     gene            complement(13423..13686)
                     /locus_tag="PLG49_03695"
     CDS             complement(13423..13686)
                     /locus_tag="PLG49_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOQ16453.1"
                     /translation="MNCWEILGINATVDVKIIKKAYAEKLKTCHPEDNPEAFIHLRRA
                     YEEALRQTKVLMISEDNEMMQSKISPLFLRKKVFLNFNLIFQE"
     gene            complement(13703..15403)
                     /locus_tag="PLG49_03700"
     CDS             complement(13703..15403)
                     /locus_tag="PLG49_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HOQ16454.1"
                     /translation="MAIVGIDLGTTNSLVAYWSAEGAKIIPNVLGSHLTPSVVSIDEN
                     GEILIGEVAKQRLITHPDYTAATFKRYMGSNKKIKLGEKEFLPEELSSFIIRSLIRDA
                     EAFLNEPVEEAIISVPAYFNDKQRKATKRAGELAGIKVERIINEPTAAAIAYGLHQKK
                     DETKFLIFDLGGGTFDVSVLELFENVMEVKAIAGDNYLGGEDFTEIIYKKFLEDNHIN
                     EKDIDNKLKSALWKQAELCKRKLGTQEGIGLIDYVIDGKKFLWKITQDEFEAIAKPLI
                     NRLRLPIEKALRDALVGVNDLEDIILVGGTTRMPLIKIFISKLFGRFPANDIDPDEVV
                     ATGAGIQAGMKSRNAAIKEVVLTDVCPYTLGTEIAKFTESGYETGHYSPIIERNTIIP
                     ASVVKRYYTIYDFQDRVEVGTYQGESRLVAQNIKLGELSVELPPAPAGQEAIDIRYTY
                     DINGILEVEVTVVSTGEKQRIVIEENPGNMTKEEIEERLKQLENLKIHPRDKTENKLL
                     LSRAERLYEECLGKVREEIADAIIKFEHVLNSQDNRLIEKAARMFKEYLDEIEKGNEF
                     "
     gene            complement(15698..16240)
                     /gene="dcd"
                     /locus_tag="PLG49_03705"
     CDS             complement(15698..16240)
                     /gene="dcd"
                     /locus_tag="PLG49_03705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOQ16455.1"
                     /translation="MILSGREIKRRLGKEIIIEPFVESQLNPNSYNLRLHNELLVYEE
                     DVLDMKKRNKAKRIIIPPEGLKLEPGRLYLGRTVEYTKTDGFVPMLEGRSSIGRLGLF
                     IHVTAGFGDVGFSGYWTLEIFCIQPIIIYPNIEICQIYYHDILGEYDRYSSGKYQNNT
                     DIQASMLYKDFEKNQVTDNL"
     gene            complement(16363..16662)
                     /locus_tag="PLG49_03710"
     CDS             complement(16363..16662)
                     /locus_tag="PLG49_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16456.1"
                     /translation="MILKAEAEGKIIGSVRAYKNNNTCYIGKLIVDPSYQNRGIGQKL
                     MKEIENSYSDVSRFELFTGFKDEKNIYLYNKLGYKVFKEEKLSPELTFLFFEKII"
     gene            complement(16683..16820)
                     /locus_tag="PLG49_03715"
     CDS             complement(16683..16820)
                     /locus_tag="PLG49_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16457.1"
                     /translation="MNIEIKIANKEDLKEILDLQHICYKENAIRYNDFNIQPLTQSFR
                     N"
     gene            complement(16884..17519)
                     /locus_tag="PLG49_03720"
     CDS             complement(16884..17519)
                     /locus_tag="PLG49_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOQ16458.1"
                     /translation="MLTMPEIKICGLKSVEQIYMINKYDIDYVGFVFAESKRQVTPEL
                     AKEMRSKLRRGIKAVGVFVDTPIEEVNQIAKYCHLDIVQLHGNEKDDECSRSIVPVWK
                     ALSVQDKEVLNRLEEYKAIQGFVFDSGKGGSGKTFSWDLIKGISSKYFTILAGGLNSE
                     NVVQGIREVNPHIVDVSSGVESKGEKDESKIKAFIKAVKTCNTFYENYPHL"
     gene            complement(17503..18282)
                     /gene="trpC"
                     /locus_tag="PLG49_03725"
     CDS             complement(17503..18282)
                     /gene="trpC"
                     /locus_tag="PLG49_03725"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOQ16459.1"
                     /translation="MILNKIVERKKESLNQLKKKASIAYFERLTDKKEIPLSFYKAME
                     KPGLSIIGEVKKASPSKGIIRQEFNPVEIAKEYESSVDAISVLTEEEFFLGSPRYLKE
                     IHGEVKLPLLRKDFIIDPIQIYEARALGASAVLLIAAILDDIQLREFISLTHSLDMDA
                     LVEVHDEDELERALNTNAVIIGINNRNLKDFSVDINTTIKLSREIPKDKLIISESGIF
                     TGEDIMKLKKANIDGILVGESFMKSEDINKKAKEFKDAYNA"
     gene            complement(18279..19295)
                     /gene="trpD"
                     /locus_tag="PLG49_03730"
     CDS             complement(18279..19295)
                     /gene="trpD"
                     /locus_tag="PLG49_03730"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOQ16460.1"
                     /translation="MLRYAIQNLLEGKHLSEQEMMEAMEFIMDGEASPALIGSFLTAL
                     RMKGETPEEITGGAKAMRKKAEKIILENFYTLDTCGTGGDCSGTFNISTAVAFIAAAA
                     GIHVVKHGNRSVSSKSGSADVLEALGANIALLPKQIEACVKDKNIGFLFAPTFHKAMK
                     HAAGVRKELGFRTIFNILGPLTNPANAKSQVLGVFDEKLTEPLGIVLANLGVEHAMVV
                     HGMDGLDEITVTDKTKVTEVKNGEIHTYYITPEEFGIQRAGKEEILGGDAKENAEIIR
                     NILDGEKGPKRDIVLLNSGAALYVGKKAKSLEEGIHLSRELIDGGYALNKLNDYIEYT
                     RSVS"
     gene            complement(19289..19867)
                     /locus_tag="PLG49_03735"
     CDS             complement(19289..19867)
                     /locus_tag="PLG49_03735"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOQ16461.1"
                     /translation="MVLLIDNYDSFTYNLYQYIGAYKKEIKVVRNDEITIEEIEKMNP
                     SHIVISSGPGTPQKAGLCMDIIKNFYKKIPILGVGLGHQCIGLAFGAKLIQSKKLSHG
                     KTSIVMHKGMNIFEEIKNPMRVGRYHSLVLDMDTISDCFEIAAITEDKEVMAIFHKEY
                     PLVGVQFNPESINTPEGQKIIENFLKKELILC"
     gene            complement(19878..21260)
                     /gene="trpE"
                     /locus_tag="PLG49_03740"
     CDS             complement(19878..21260)
                     /gene="trpE"
                     /locus_tag="PLG49_03740"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOQ16462.1"
                     /translation="MIYKLYSKKLDGDTQTPITIFNKYVGKEKGFLLESRDNKKGRYS
                     FIAKRPFAEIQGRDREVTIIEGDKVIKKQGKILDIVKEYMSAFNVKPSDFIPFMGGAV
                     GTVSYDLIRQAEKLPPANKDVIGVPDLHFMITKEMIAYDHFHQQIYLLVIEEESDEGE
                     KRAKEKMQQMEEELLKEEEIEAIKDKVKYKKISSNMTKEEFIEKIKKAQKYMEEGDIY
                     QIIISKRWTMETDEDAFVLYRRLRRINPSPYLYYLNFGDYQVAGSSPETLVELRKDRV
                     YTCPIGGTRKRGKNHEEDEALKAELLNDEKDKAEHIMLIDLARNDMGKIAQIGSVQLT
                     RYMDIHYYSHVMHMESQIEGIKNEKEDAFSILQSFLPAGTLSGVPKIRSIEIIDELEE
                     EKRGIYGGAVGYISFDGDMDMAIAIRTMVIKDGKVYMQAGAGVVADSDPEKEDEESEN
                     KIMALLKAVE"
     gene            complement(21688..22386)
                     /gene="cwlD"
                     /locus_tag="PLG49_03745"
     CDS             complement(21688..22386)
                     /gene="cwlD"
                     /locus_tag="PLG49_03745"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR02883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HOQ16463.1"
                     /translation="MDFWKKNPKLCTSVALFLVVLFAYNIYTNRTVSTFALPTAKRVI
                     VIDPGHGGFDPGKLGIHGAHEKDINLAIALKLQAYLEQSGAFVVMTRTSDEGLNEEGD
                     NNKKRSDMRRRKELINSSEGDILISIHQNSFSESKYKGAQVFYHGHSKEGERLAKSIQ
                     KQLREFVDLTNKREAKANTDYYVLKTTNIPAVIVECGFLTNPEEEANLNNEIYQEKIA
                     WAIYLGIVEYFKEI"
     gene            complement(22465..24159)
                     /locus_tag="PLG49_03750"
     CDS             complement(22465..24159)
                     /locus_tag="PLG49_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOQ16464.1"
                     /translation="MKKKKRSFGCLLFMVIIAVSLLLVKKYMPNFSYISAYEAFSELS
                     EGKVGLVLGKEIIAMEHSPQVIDGEIFLPFDFVRTYIDPYIFWDEGTQKLTITTQNKV
                     IRMKTDELTYYINQEPLELQVPLRKFEEVPYIPFNFLKEWFNIEAVYYKESNMVVMDY
                     TTETKIIGEVIKKNAPIRYENHIKSPILVKLSLSDKVRIFEEAGDWYYVRNEHGIVGY
                     ISKKHIGTIEEIEGKAVQKEESFPTWEPLQGKINLVWDQVFKVESSEVPSKFEPIYGL
                     DVLSPSWFEIEDEQGNIRNIASKRYVDWAHSQGYQVWAMITNPFSDPDLTHKVLSNTD
                     SRENIIKQLLAFASIYNLDGINIDFESLKKETGEYYVQFIRELTPLLKEQGLVVSVDM
                     YVPSGWTRHYNRSEVGKVVDYIIIMGYDEHWSTSPESGSVASIGWVEQGIKNTLEEVP
                     HEKIILGIPFYTRLWAEEEVEGEIKVSSKAYGMSRGEQFLKDAGVEPVWLEDAGQYYG
                     EYTRGDVVYKIWLEEERSIEEKLKLVQKYDLAGTAGWKRGLEKPEIWKVLYKYLKE"
     gene            complement(24408..25319)
                     /gene="trxB"
                     /locus_tag="PLG49_03755"
     CDS             complement(24408..25319)
                     /gene="trxB"
                     /locus_tag="PLG49_03755"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ16465.1"
                     /translation="MYDVIIIGAGPAGMAAAIYAGRAKLNAVMFEKEFVGGQVTKTYE
                     VANYPGIADVIGPELALKMQAHAKEYGIEPIVEEVIDIEPEGKIKKVMTKNKTYESRT
                     ILLAMGASWREIGVPGEKKLKARGVSYCATCDGAFFNGKNTVVVGGGNRGVEDAILLA
                     KFSPKVYLVQDLPHLTAQKILQEKLSSLDNVEVLTNQQVLEILGEDKVTGIKIKDKES
                     GEEKILEVEGAFIAVGMTPNSEIVKGKVKTDQWGYILANENCETDIPGVYAIGDIRDK
                     KLRQIITAAADGAIAINAVEKYLIENP"
     gene            25559..26200
                     /locus_tag="PLG49_03760"
     CDS             25559..26200
                     /locus_tag="PLG49_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HOQ16466.1"
                     /translation="MRIEKISDTQIKIILNQADLKNRDIKISELAYGSKKAQELFRDM
                     METALEEHDFDTHNVPLMIEAVPLSVDSIMIIVTKVEHPFEIGEKITPFRSKSNTRTF
                     KKKTFVPEEEEDSTLMIYSFEKLDDVVEASNRIYHLYCGANSLYKDVYNNRYFLVLHK
                     NYFVDTNLNQIESILSEYGNKHISTPLTEGYLAEHGEIMIQNKAIEVLKKHLA"
     gene            complement(26218..26433)
                     /locus_tag="PLG49_03765"
     CDS             complement(26218..26433)
                     /locus_tag="PLG49_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOQ16467.1"
                     /translation="MAAKVTKDMIIADIIAIDRGLIPILMEAGMHCIGCPSSQGETLE
                     EAGFVHGLNVDEVIEKMNAYLAEKEKN"
     gene            complement(26514..28139)
                     /locus_tag="PLG49_03770"
     CDS             complement(26514..28139)
                     /locus_tag="PLG49_03770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOQ16468.1"
                     /translation="MDKQIFVFGHMNPDTDSICSAIAYTNLKKELGYKNVVAARLGDI
                     NRETKFVLDYFDVEIPVLLENVEPQVCDLNFYVSELVRKDDSVKTVWELMKNNKRKMF
                     PVVDEKNRLTGVVSITDIATAYMELEDEMALKNQRAKFSKLIEALDGEVIAGSYPYEY
                     VEGNIYTDSTLDEDQILTKGDIVITGHVKKLHKRVIKSGAGCIIVAEGKTSKKLFDSE
                     EELTCAVVTVPHTFFKTIKLINQSIPVKSIMKKKNLVYFQMDDYIDEVKEIMQSSVYR
                     NFPVVDSEGVVKGVISRRNLIDINRKNVILVDHNEKGQSIKGLEKANILEIIDHHRVA
                     DIYTMSPLYFRAEPVGSTATIISKMYKENNITPTKSMAGIMLSAILSDTLIFKSPTCT
                     EEDKKEAEYLAKIAGVKIKSYGMKLIAAGTSLEGKTAEEIYYSDMKNFVFGHYNIVIS
                     QINTADFKGIFDLITDIKEIMKKLCEDKKYDLALLMVTDIILGGTELIAIGPAKELAH
                     KAFGMGLKEDSIFLPGVLSRKKQVVPRLMNAVQ"
     gene            28668..30896
                     /gene="pflB"
                     /locus_tag="PLG49_03775"
     CDS             28668..30896
                     /gene="pflB"
                     /locus_tag="PLG49_03775"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOQ16469.1"
                     /translation="MFEQWRNFREGNWVKEIDVRDFIQKNYAPYDGDDSFLAGPTKRT
                     LKLWDKVMELTKVEREKGILDAETKIPSGITSHGPGYLDKELELIVGFQTDKPLKRGI
                     MPFGGIRVVEKALNAYGYELDPATKEIFKSYRKTHNDGVFDAYTEEMRKARHSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDKLIEEKQKEKRQLALEHMESGVIRLREELSEQIKA
                     LHELKEMAQSYGFDISQPASNAKEAIQWTYFAYLAAIKEQDGAAMSIGRVSTFFDIYI
                     ERDLKEGILTEEEAQELIDDFVMKLRMVRFLRTPAYNELFSGDPTWVTEAIGGMGLDG
                     RTLVTKTSFRILHTLNNLGPAPEPNLTVLWSNHLPENFKKFCAKTSINTSSIQYENDD
                     LMRRWYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEISGEQVAPPF
                     APITSEYLDYDEVMSKFDQILDWLSLLYINTLNIIHYMHDKYAYERLQMALHDMDILR
                     TSACGIAGLSVVADSLSAIKYSKVKVIRNEQGLAVDYEVEGEYPAFGNNDDRVDSIAY
                     SIVKNFMNKLRKHKTYRDSIPTLSILTITSNVVYGKKTGSTPDGRKAGEPFAPGANPM
                     HGRDKNGAIASMASVAKLPYQHAEDGISYTFSIIPKALGKNLDERINNLVGLLDGYFH
                     DDGHHINVNVFDKETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHE
                     RF"
     gene            30975..31703
                     /gene="pflA"
                     /locus_tag="PLG49_03780"
     CDS             30975..31703
                     /gene="pflA"
                     /locus_tag="PLG49_03780"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOQ16470.1"
                     /translation="MSVTGRIHSIETCGTVDGPGIRFVIFTQGCPLRCKYCHNPDTWK
                     ISEGKNMTVDELIQEIEKYKSYMDFSGGGVTVTGGEPLMQPKFVKELFRQCKERGIHT
                     ALDTSGYIPLEHAKEVIQYTDLILLDIKSFDYQTYKELTGVSNEPTLELAKYLSDIGK
                     PMWIRYVLVPNLTDNPDHIEALAQFIKDLKTVEKIEILPFHKMGEYKWEELGYNYELK
                     ETPAPSKELVDSVKEIFKKYNLKV"
     gene            complement(31849..>32315)
                     /locus_tag="PLG49_03785"
     CDS             complement(31849..>32315)
                     /locus_tag="PLG49_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16471.1"
                     /translation="LVVFAIIVATYVLTAVGIYGMAKKKDIENAWLAWIPIANYYILG
                     KVIGPAKINKIEINNTEVVLPLAQLAVFGISVMPVIKIIATPLNIIFSACVVYTLYKM
                     YHEEVSIPMIIIWTLFTPIFLFTLRDKEAIRAANEDVSSNSQQYNSQDPMNM"
BASE COUNT         9836 a         6510 c         4637 g        11232 t
ORIGIN      
        1 atcaaaagcc gtctttttta cctatggcat attattgaat cagaatatat aataatgatt
       61 aagtataaag aaatttccgg aggtgctgta tggttaataa aaagctgcct aaagttgcat
      121 atttctgtat ggaatatggt cttgattcat cttttaaaat ctatgccggg ggtcttggaa
      181 ttctggcagg agattattta aagggcgcca aagattataa ttaccctatt gtagggatag
      241 gaataaagtg gaaacaaggt tatacagacc agtttattgg agaagatgga cgtccttatg
      301 attgctatta taactaccaa tatgacttct tgaaagatac cggagtcacc gtatccgtaa
      361 aaatccgtca gcgggatgtt ttatgtaaag tttgggaagt tactggcttt gacaatgcac
      421 ctctttactt gctggataca gatttgcccg aaaatgaaga tgcctggatt actggtcaat
      481 tatatggctg gtttggagaa gaaagaattg cacaggaaat ggttcttgga ataggtggaa
      541 tacgagccct cagggcactt ggaattaacg tcgatgttta tcatttcaac gaagggcatg
      601 ctctgtttgc aggctttgaa ctcgtcagag aaaaaatgga ggctggtatg agctttgaag
      661 aagctgtagc agcttcaaaa gatgaaattg tctttacaac tcatacccct attatccagg
      721 ggaatgaatc ccatcacctg gacagaatca tgtatatggg ggcaaataac ggtttaactt
      781 tagaacagct ggtatccctt ggaggagctc catttaacat gacggttgct gccttaaggc
      841 tttccagaat ctccaatgcc gtttcccaat tgcactgcaa aacggccaat aagatgtggg
      901 cacatatcaa caatcgttca gaaattattg gcatcaccaa tggcattcac ctgcctacct
      961 gggttgatga gcgtatgatc gagtgtgccg aaaaaggcgg tgatttatgg aaacttcata
     1021 tggataataa aaagaaatta attgaatttg tcaaagaacg caccaatgta gaattagata
     1081 tggataaact cctgataggt ttttccagaa gagccgctcc atataaaaga agtaacctta
     1141 tctttaccga tgaaaaagtt atagcacctt tacttaaaga aagaaaaata caaatcgtct
     1201 tctctggtaa agcacacccc ttagatgaca cgggtaaaaa aaacgtagaa aacattgtcg
     1261 ccatgaccaa aaaatatcct caatctgtag ttttccttga aaactacgat atgaccatcg
     1321 gagcaatgct gacaaaaggt gccgatgtat ggttaaataa tccaagaagg cctaaagaag
     1381 ccagtggtac ctctggcatg aaagctgcta tgaacggtgt cctgaacctc agcaccttag
     1441 acggctggtg gccggaagct tgcaaacacg gagtaaatgg ctggcaattt ggagatggct
     1501 ttgaaagcga agatgaaaaa gaattggatg ctcatgactt aaaagccctt tataaagtcc
     1561 tcaccgaaga agttattcca acctattatg ataggcggga taaatgggta gaaatgatga
     1621 aagacagtat tctgtcaaca aaagatacgt tttctgttaa aagaatgctg gaagaatatt
     1681 atgaaaaact ttatataaaa caagaagaaa aataataaga cagcccacaa ggctgtctta
     1741 tatattatcc cttttttggc tcccacttat atggtagggt acaccgcttt tttggctccc
     1801 acttatcggt agggtacacc gctttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnggt agggtacacc gcttttttgg ctcccactta tcggtagggt acaccgcttt
     1981 ttcattttat agattttaaa attattcttt cagataacgc ctgctatata gcaaatttct
     2041 ctttctttta atccctgctt acttacaaag tatataaatt ttttataaat atgtcaagtc
     2101 tttcatgctt ttatgagcaa ttcagttgaa agacgccaac acttgaacta aaaaatcata
     2161 acttaattct tctgtggaat tcaccattac atctgcctca acacctacgc ctactgcaat
     2221 catcccagct ttttggatag cctctacccc tgccctggca tcttctatag ctatggcttc
     2281 ttgcgccttt aaccccagac catttaaagc gtccaaaaaa atatccggtg caggtttgcc
     2341 atttttaata tgagcaggat taacaatata gtcaaaataa gcttctatct ccagcttctt
     2401 taaaatcatg ggaccatttt ttgaagcaga agccagggca atcttaatct gatggtcttt
     2461 taattcctga agaagctttt caatgcctgg taagagatcc tttttcgtca tttgcttgat
     2521 tgattcttta taataattat ttttcttctc tgccaaagcc atcttttctt cttttgtata
     2581 aagatcagct ttattgccat gttctaaaat aagctccagg gattccattc tggaaatccc
     2641 cttcagcttt tcattaaaag tcctgtcaat ttcaattcca atctcttttc caagataagc
     2701 ccaggcttga taatggtatt ctgctgtatt ggtaatcaca ccatctaaat caaatattac
     2761 ccctttaagc attacaggcc tccattttaa attaatttac aagaaatttt atatataaga
     2821 tcatattcaa tattatatag ccatagtttt aagggatccc ccttttccag agtaatggtt
     2881 acactctccc tgtttacatc aaccttaatt aaccggtctc tataattgat tttaaaggaa
     2941 taacgctccc aatcctttgg aataaaaggt gaaaaagcta atttttcacc aaaagttctg
     3001 agccctgcaa atccatgaac aatagaaagc caactgcctg ccatggaagt aatatgaaga
     3061 ccatcttctg tatcattgtt ataattatca agatcaaggc gcgctgttct tttatacatt
     3121 tctactgctt tttcttccat ttttaattct gctgccagga tacaatgaat acaaggagac
     3181 aagctggact catgaaccgt catcggctca tagaattcaa agtttctttt cttttctaca
     3241 aaagtaaatt tatgatttaa aagatacaag ccctgcagta catctgcctg cttaatgaag
     3301 catgaacgaa ggattttatc ccatgaccaa ttttgattaa ggggtctgtc tccctccaat
     3361 aaatcctcca cagtcattaa ttctttatct aaaaaagtat catgttgaac gaaaattccc
     3421 cgttctttat cataaggata atacattttc ttaatgatat ctctccattt atcttcttct
     3481 tcctgactta gctggtgaaa ttcaacccta tggtcctttt ttatttttct taaataatca
     3541 atcacttcta aagtatattc caaagtccag gctgccatgg tattggtata ccagttatta
     3601 ttgacattgt tttcatattc attaggtcct gttaccccat gtatcatata gacatctttt
     3661 ttcttatgat aatgcacccg gtctgcccaa aaccttgaga tacctattaa aacatctatg
     3721 ccatagtctt ctaaataagt cttatcccct gtatagttta cataattata aatcgcataa
     3781 gctatggctg catttctatg gatttcttca aatgttattt cccattcatt gtgacactct
     3841 atgccattaa aggtcaccat gggatagagg gctcctttta agcctaactg cctggcatta
     3901 taataagcct cttctaattg attgtagcga taaagtaaaa gatttcttgc gacttcttta
     3961 tcggcaactg acaaataaac cggaaagaca aaagcttctg tatcccaata tgtggcgcct
     4021 ccatactttt ctcctgtaaa acctttaggg ccaatattta atctgggatc ctccccgtag
     4081 taagttgaaa acatttgaaa aagattaaaa cgaattccct gttggtcagc cgtatcgccc
     4141 ttaatttcaa cgtctgcctt ttcccaacgt ctttgccatg cttccatttg ctcttttttt
     4201 aggctgctga agcctttctt ttctgcctgg ccaatcagtt ttaaagattt atgagcaagt
     4261 tcttcttcct tataatccct ggaggtcaca accgatatat atttaatgat tctgaccgtt
     4321 tcattttcct ttgctctaaa agtcattttg ttggccacat atttgtcttt ttcaatgatt
     4381 tcaatagcac aatccttaga gctttctatc ttcattctgt tacatactgc aaatctggga
     4441 accccaaagg gattttcttt tgtcttcatg attaaaaaac cttctttaac acttacgccc
     4501 ttatctgttt caagccagaa tttttcatta taattggagt cttcattaat gacatccccg
     4561 tctaaataag gaataaaagt aatctccccg ctataattta atggggtcgc ctcataggaa
     4621 ataacacata attctttttg gacgatactt aaaaatctgg tcacttcaaa ttttgttctc
     4681 tttccattta tattactaat aaaagttctg taaagaatac cgtttttcat atctaattct
     4741 ctataatatg cttcaagagg aaccttaaac agatccagtt cttctccatc cagtaaaaga
     4801 tcaattccaa taaaattggt actgttgatg acttttccat aatatttcgg ataaccgttt
     4861 ttccaccaac ctacccgggt aggatccgga taccaaacac cgccaatata agtacctctt
     4921 agcatatctc cactatactt ttcctcaaaa ttgcctctca ttcccatgta gccgtttcct
     4981 aaagacataa cactttctgc cagtctaaag ttttctttat gtaaattact ttcaataatt
     5041 ttccactcat caactccaaa tatcgtcttt ttattcatcc tagttactcc tttaaatttt
     5101 attatttttt attaaaaaca tatgcggata tctagctgac aggcagtaac cgttcaacaa
     5161 tttccataaa acctttttca ctggcatatt gtatgggcgt tttgccataa ctgttttcaa
     5221 ttttggcatt taaatggtca tatgtaagca gtaactctac aatttcttta tgaccataca
     5281 tgcaagcttc atgtaaagct gtatcacctg ttttgcttct tgcattaata tccagacctt
     5341 tttcaataaa taatttaacc atctcttttt tattattcct gcaggcttcg tgcagtatag
     5401 tccgtccaaa atcgtctgta gcgtatatat tggctccggc atctatcaat aattgtacac
     5461 tttcaggctg tgtattttta caggcataga aaagagccgt ataaccttta ttggtcgtat
     5521 gattaatatc tatgccattt gataataata tctttatgat ttcttttctg ttattattac
     5581 atgccaaatg aagagctgtt tctccataat tgttcgtgta attgatatca acgccattca
     5641 atataaataa ttctgcaagc tctttttgcc cattcatgca agcgtaatga aaaggactta
     5701 tcccatcatt atttgaaata ttaacatttg ccccttgttg aataagcagt tttgcagttt
     5761 ctttttgccc ggactggcag gcaagaaaaa gagctgtttc cttatcattg ttgactaaat
     5821 ctttattagc tcctttattt aataacagca gcacaatgtc tttacagcca ttttcactgg
     5881 cataatgcaa ggctgtattc cccttttcat ctctataatc taagtttaag cctttttcaa
     5941 taagcatttc tgcaattcct aaattaccat tttgaatggc cgctaataat gttttttcca
     6001 ttttcatcac ctttaattta atactctaac aataatttaa ccagagcatt ataattatgt
     6061 tccacagcaa tatccaaagg cgtctggccc ttagaatttt tcacatgggg atttgctcct
     6121 ttttgtaaga gcatccttac tatattacga ttaccttccc ggcaagcaaa gtgaagcggg
     6181 gatatcccat catgatttgt actgttcaca ttagcagctc ttgaaagcaa tagttctaca
     6241 attgcaggat cattattatc gcatgcaaac attaaggctg tattaccatg attatccgtc
     6301 atctctatat cggcttctgc atttagtaag ctttcaacta atttttcatt cctgctcctg
     6361 caggcaagca taagaggagt aagtcccctg ttatcggaaa tattaacatt cgcaccgctg
     6421 taaataagaa tctctgcatt tttcttattc tgttcatggt ttacatttct atgaagacaa
     6481 taaggtccaa aatagtaatg acctgattcc agacatacat taaagagagg tgttcttcct
     6541 tctgcattgg taatattgac atccccattg cctctgtgaa gaataagttc tatgcactct
     6601 gtaagaccaa gaagacaagc ataatgaaga ggcgcattcc caccaaaatt ttgttcattg
     6661 acattaaaag gatgattaaa attgagaaag ctttctatat ttcctgtctt accataaaga
     6721 gcagcaatgt aaaaagctgt acaaccatct tcatctttaa tactattgtc agccccaaat
     6781 tccaataaaa gttttacaat cttcgggttg tttccctttg ccgccaaatg caaagccgtt
     6841 tgatttctat cgtctgtagc attcacttct gccccgctgg tcaataacaa ttcaagaatt
     6901 tcttcttgat tacaggcatg atgaagaacg gttttagaat tatcatcttt cacattcaga
     6961 tctgctccat actgaatcag catttggact atttctttcc ttgcatccca gcaagcttca
     7021 aaaatagaag ttttatccct acatcttgca ttgacatctg ccccattgga aagcaaaact
     7081 tctaccaatt ctttattgct catcctgcac gccctataaa gtaagctttc atcatttgca
     7141 tttttttcat tgatatcagc tcctgcctca atcagcagcc tgataacctc ttttacagaa
     7201 tatctgcaag ctactcctaa agccgtttct ccattggcat tttttgcatg aatatccgct
     7261 cctgcctcaa tcagcaatcg gacaatatct ttcgaaccat ttttacaagc aatttctaag
     7321 agactttctc catccctgtt tcttgaatga atgtctgctc cattcaaaat aagcaattgg
     7381 gctatatcat gatttctatt tttaacggct gtaaataaag ggctttctga aaaatgattc
     7441 ttgttctcta tatctgcacc acggcttaac agtagcagta ctaaatcttt atcccctttc
     7501 tgacaggcaa ggtgtaatgc tgtatctccg gaattattgg tgatatttac atccgtacca
     7561 taagacatta aaatatctgc gatgtcttta ttgccttgtt ttatggcaat atggagagca
     7621 gtttccccct gattattctt ttcattggca ttggcaccat aagaaagtaa taattcaaca
     7681 atatctttac tgccatcatt gcaagcatat attaagggtg tattcctgtt catatcttga
     7741 ggattaagct cttttgcata ttgtaacagt aattcaacga tatctttatt ttgcttttct
     7801 acggccagat gcaaaatact tttttcatta cccagggttt ttacgttaac atccgcatga
     7861 acttctaaaa gtaattctgc tatttcctta ttgccagtct cacaagccag ataaaggggc
     7921 gtaatatttc ttctgtcttt tacattaata tcagcattat ggtgaataag taattgaacc
     7981 atttctgtat cttcattctg acatgctaaa aataatacag tcctttgatc ggatgctttt
     8041 ttcaaattta cattcgcatt aaaatttaga agatactcaa agatatccct tttacgtctc
     8101 tcataaagca taaaaattaa aggcgtcccc attttattca cataattaat atctgctcca
     8161 tgactgataa gaaaatcaat tatttctttc tgaccatgaa gacaggcaga agtcagggga
     8221 atatcaccaa aactgtctgt aatgttaata tcaatacctt gatggtataa agcttcgaca
     8281 attttctgat gtccctttga acatgcggtg tgaaatatcc atgcaccaat atcattatta
     8341 aaatcatcca taagaccagc ttctaacagc atttcagcaa tatcatactg cctgctgtta
     8401 tatgcagctt taaggactgt tgtcccgtca ttattttttg cattaatatc tgcaccttta
     8461 tccattagta attgaataat ttctttatta ccccgggtca ttgcataatg taaagctgta
     8521 tttcccttat tatcagataa attgacatct gcacccttat gaattaataa tttgcctatt
     8581 tcaatcttcc ctaaataaca tgccaggtga agggctgtca taccctgatt attcatttca
     8641 tcaatattta tccctttttc aataagaatt tctgctattt cttttctgtc ttcctgacat
     8701 gcaaggtgta aaaagctgtt tcctgacgga tcggtaaaat gaatatccgc cccatgttct
     8761 atcaataatt ctatcatttc tatatcttta taacggaaag tttcctgaag ggctgtccga
     8821 ccttgataat ccttaatatt gacatctgct ccatactgaa gtaagagtaa aattaaatct
     8881 ttatctcttt ttaaacaagc ctgatgcaac gcagtctttc catttttatc tacagcattt
     8941 acatcgatgc catttttaag gaataattca gctattaata aatctttaat attagctgcc
     9001 tgatgcaata agctttcatt atttttattt ttaatatata tgttggcacc attcttaata
     9061 aaaatttcag caatatcaag aaagccattt tggccagctt tatataaagg cgtttccccg
     9121 tccttatccg gctcatctgc aggaacgcct gcatcaatca gtaattgaac tatttcggta
     9181 tatcctttta aggcagcaat atgcagcgca ttccttccaa acatatcttt tacagtgata
     9241 tcttctttct cagataagta ttgctttatc tcttcaagct tccctgcctc acacaagttc
     9301 aggaaattat tattcatcta tttccctcca aaatcagcta ttagaaaatc tttttaaata
     9361 tccaacgcaa tatcccaggg aattttcaac cagggcgaat cttctttaaa aagaagtctg
     9421 cgaagcaaat ccacatgcca ttttacattt tcttctgtca gcttatccaa ccaggctaat
     9481 cttccaacca tataagccaa accatattca taccagctgg aataagactg ttgagccatc
     9541 ggaccaattt tagccataag ctcccaggct tctttatcac taatatattt gattaaggcc
     9601 ccatcccggc ataaagtaat atatctgcca aaatcccagg ccgctattcc tgccctggga
     9661 agaatatatt tatttaattt tgataactga taactattat aaagagaagt ccctgaagga
     9721 atcctgttta attctttttc ctctaatgaa taaggcaata aggatatgta acgaaaattt
     9781 ctgttaaaat ctgttctgtg ccctgaatgt aataaccagt taagagaact gtataagtct
     9841 tctctattac ggattcccca atctctctcc aatgcttgtt taacgagata gatatgttct
     9901 ttcgttgctt cataaccgta taaagtatcg tgacgtacct cattttgttc tgtcaggaca
     9961 ccacttaata acaatagcca taactgagtc tgagttaagc ggtctggatt ataacgatac
    10021 aagtttttca cccccgtgat taataccttt atgctttctt attctctgct ttactgtttt
    10081 gcttctttaa tgacagcttt atactcctcg tcaaaatcaa aatcactgaa aaacacatta
    10141 acagctgatt tatctttttt ctctccatct tcttgaatct ctgctaagag ctttttaatt
    10201 tttttgtaat tagatttatt attcatttta tccattaaaa acagggtata aggaatgcat
    10261 tccttagcat aacaagcata ataactttct ttgaatttga gtgctttttc aaaatacttc
    10321 agggcttgtt ttaattcttt tttattgcat tcagcataat gacctgaaag aaatttttga
    10381 ttaaattctt ttaaaatggt agcccctctg atacataata tcgctggata actcatattt
    10441 ccaacagaac ggccaaacac cctggcaatc tcttctgcct catccagcac tccttttgct
    10501 ttgtcaaatt gccccaggct ccctaaaatc attgctttat tgcaaataca tgcaattaaa
    10561 cctacttttg aaatgaccgg gcaatcgttg cgttttttat aaaagtcaag gaccttatcg
    10621 catatttcaa taagaatgtc tttatctact ttttccattt ccagttctgc tgaacctata
    10681 gcaactaaag cattaaaaac cgcttctgaa aagttaagat tctccgaatc caagtattct
    10741 ccggaaaatt cattaaccca cttaaaaatc tcatgatagt ctccttctgg tctactgatt
    10801 ttaaacttac gggataagtt caacaaattg ataacaaacc atttttgaat ttcataatct
    10861 ttaaagtcct tatacttttg cattaattct ttgctatggc gaagccattc atgaaaatct
    10921 tcaatcgtac ataataaaag attcttagca catgataatc taaatgcata tatgccgaaa
    10981 tgagcgttat ttttcttttc taagcaggcg agcatttcat cagatacaac cagcgcttta
    11041 tccaccaatg ctttattgtc ttttgtgtaa ttgactgtaa tgaaccttac caggcataaa
    11101 aatatcctga tcagcttaat ataaatggca gaatctatct ctttctgctc ttgcatatga
    11161 ttgatgtatt gaattattaa atataagtca cataaagctg tttcattaaa tctcatttct
    11221 attaatatct caatccagtt taattgtcct aaaatgttat ccttgctaag actaagctga
    11281 cagatatctt ctaatttata ttcaaactta tcctgtacaa attgagagaa taatttcctt
    11341 tcttttgaga ctggcatagc aatctccttg ttatcaccaa acataagttt tgaaatatct
    11401 tctttcccgc ttatgtaatt taccaactca tcatatctgc taggattttc ttcagctaag
    11461 ctttttaaat gacttaataa tataaacaga tatatatcct gataatcttt actcatttga
    11521 ttttttaagg aatcacaata atcatcaatt aataaaaaag catctttttt gcttgttaat
    11581 tgcatcaaga cttttgcgat cataacttgt agccattcat cagcagcatc tatattcatc
    11641 attcttttaa gataaattag agtcttatcg ggcatgcctt tctcatagca ggcacaaaaa
    11701 gcagcttttg caagactaaa gctggtttga agcctgtcag aagcagtaca aaattctgtt
    11761 atcgccttac ttttattatt cattaaataa taaagacagc ctgacattaa gtaacctgca
    11821 aatgaatttg ctttcgcctt tttatatgtt tcaatatctt tttgagctga ataataattt
    11881 tgcattgtta tataattctc tgccctgaat aaataaaacc aaattaaatc atcaaaccct
    11941 acagcccaat caattgctac tttaaaactt tctttagcct tttgataatc acctgacttc
    12001 aagtaactgt aagctaaaca aaaccacatt tgtatatgct ccggattttg ctctacaatc
    12061 atttgaagtt catctctggc tttttcataa tcaaaattat aaaaatgtat aacacctttc
    12121 agggcatgat atctccattt gtcaggatta agcccaattg ccctgttaac cttttccaga
    12181 ctttctttat acattccttt tattcggagt gcatcggcta attgataaag caattctgca
    12241 tcatatgggt ttatgttaac tgcagttaag aaatcggata ttgccttatc caaatgatcc
    12301 attgcaaaat aacaattacc ccgttcaacc aatatttcat acccgttttc tttattgata
    12361 taagccaaat ctaaggcctt ttctaaccat acaagtcctt cttcgtattt ttctaatcta
    12421 cataaagctt ttcctgccaa aagatataat gatgaattaa ctttaggact attaagcatc
    12481 ttttgaatct cttcaaaaca tttgtcatac cagcccagtt cgtaaagaag ctgggcaatt
    12541 gttattctta agctatcctg ttcagcttct tcttgcagtt ttagtctgta atgattaatc
    12601 agctgctggt caatgctgta aatttgtaaa tatgcatgga tatcacaggg ataaagctct
    12661 ataatatatt caaaaacggt tttggcttct aataaatttc ccaacgccat ataacatctg
    12721 gcaagaccac ttaaggcatt aggatcctga ggaattcttt caagcagcca ttgataatct
    12781 tttaaagcat ctttatactg tctgagttta atgtataaat ctgcccttct gaaataagca
    12841 tctatatcat cccttttaat ctctaataag tcattgaact cctttaatgc cttctgataa
    12901 tctcctgttt cacaataata aaaacctctt atgcggatta aatcataatc ctcactatag
    12961 attgcctgag cctgatcaat gctttcttta gccaattcaa aattcccatg aaacaaagcc
    13021 caaaaagcct gctccctgta aagaagaaag ctgtcataat caaaaggctt gtccacaggg
    13081 aaattgtaat taaattcaac ggtattatct atttcatcaa gcaaaaaatc tacatgatca
    13141 ccgaattttt tgtataaatg ttctctttgc ttagtccata aaaagcattc atctaataac
    13201 aaccatattt tatgaggaag atgataatga tttaataaaa atccaaaaag ttctgtaaaa
    13261 aggatctgtt taacatccag cctccagtaa ttttcattat tgagcaattt ttcccattga
    13321 ttgatatcta ttcttaagga gtaattttca tatatttcct tcaccttatc cataaactga
    13381 ctggcaattt gaagagcaga caaatcactt ttttatctat ttttattcct gaaaaattaa
    13441 attgaaattt agaaatactt tcttcctcaa aaacaaaggg cttatctttg actgcatcat
    13501 ttcattatct tcagaaatca ttaatacctt agtttgcctt aaggcttctt cataagccct
    13561 tcgcaaatgt ataaatgctt caggattgtc ttccggatgg catgttttta atttttcggc
    13621 atacgctttt ttaatgattt tcacatcaac tgtagcattg atgcctaata tctcccagca
    13681 gttcattgaa tccctcctac ttttaaaact catttccttt ttcaatttca tctaaatact
    13741 ctttaaacat tctagcggct ttttcaatta acctgttatc ctgactattc aggacgtgtt
    13801 caaattttat aatggcatct gctatttctt ctcttacttt tccaagacat tcttcatata
    13861 atctttctgc tcttgataat aaaagcttat tttctgtttt atcccttgga tgaattttaa
    13921 ggttttccag ttgctttaat cgttcttcta tttcttcttt tgtcatattc ccaggatttt
    13981 cttctataac aattctctgt ttttctcccg tactgacaac tgtcacttcc acttctaaaa
    14041 tcccattaat atcataggta tagcggatgt ctattgcttc ctgaccagcc ggggcaggag
    14101 gcaattcaac gcttaattct cctaatttaa tattttgggc aaccagcctg ctttcccctt
    14161 gatatgttcc cacttcaacc ctgtcctgaa agtcatatat ggtataatac ctttttacaa
    14221 cactggcagg aataatcgta tttcgctcaa taataggcga ataatgccct gtttcatagc
    14281 ccgattctgt aaacttggca atttctgttc ctaaagtata agggcaaaca tccgttaaaa
    14341 ccacctcttt aatggcagca ttcctgcttt tcattcctgc ctgaatacct gccccagtag
    14401 ctacaacttc atcaggatca atatcatttg caggaaagcg tccaaataac ttactgataa
    14461 atattttaat taagggcatt ctggttgtcc ctccaaccaa aataatgtct tctaaatcat
    14521 taacaccaac aagagcatct ctcagagctt tttctatggg taagcgaagt ctgttaataa
    14581 gtggtttagc aatggcttca aattcatcct gtgtgatttt ccataaaaat tttttgccgt
    14641 ctattacata atcgataagc cctattcctt cttgggtccc taactttctt ttacataatt
    14701 ctgcttgctt ccataatgca gatttaagct tgttatcgat atctttttca ttaatatgat
    14761 tatcctcaag aaacttcttg tatatgattt ctgtaaaatc ttctccacct aaataattat
    14821 ctcctgctat tgccttaact tccatgacat tctcaaataa ctccaaaaca gaaacatcaa
    14881 aagtgccccc acccaaatca aaaattaaaa actttgtttc atctttcttt tggtgaagtc
    14941 cataagcaat ggcagcggct gtcggttcat taatgattct ttcaacttta atgcctgcca
    15001 actctcctgc ccttttagtg gcctttcttt gtttatcatt aaaataagca ggaacactaa
    15061 taatggcttc ttcaactggt tcattcaaaa aagcttctgc atctctgatt aacgaccgga
    15121 taataaaaga tgataattcc tcaggcaaaa attctttttc gcctaattta atttttttat
    15181 tgcttcccat ataacgctta aaagttgctg ccgtataatc cggatgcgta attaatcttt
    15241 gctttgctac ttctccgata agtatttctc cattctcatc tatgctgaca actgaagggg
    15301 ttaaatgact gcccaaaaca tttgggatga ttttagcacc ttctgctgac caataagcta
    15361 ccagactgtt cgtcgttcct aaatctattc caacaattgc cataataaac ccctcctgat
    15421 cattccgtta tcgctttcca ataaataaaa agcattaaat gcaactgcaa ttactatggt
    15481 gatggtcctt acaacagaac tcgttatttt tgtatttctc atccttttaa ataaaaaatc
    15541 aaccaaagaa attttcaatg attaaaatta aataccatat aatcattgta ttaattattt
    15601 tattaatact aatataacta taaaatattt ttcaactatt ataaataaaa aaacaccaaa
    15661 attatattaa ctttaatata atccggtgct atttatttta taaattatca gtaacctggt
    15721 ttttttcaaa atccttatac agcatacttg cttgaatatc tgtattgttt tgatattttc
    15781 cactggaata tctatcatat tcccctaaaa tatcatgata ataaatctgg cagatttcta
    15841 tattcggata aatgatgatt ggttgaatac aaaatatctc taaggtccaa tagccactaa
    15901 accctacatc tccaaatcct gctgtaacat gaataaataa gcctaatctt ccaatggaag
    15961 atcttccttc cagcataggt acaaagccat ctgttttagt gtattcaacg gtccttccta
    16021 aatagagtct tccaggttca agctttaagc cttccggagg aatgataatt cttttggctt
    16081 tatttctttt tttcatgtcc agaacatctt cttcatacac cagtaattca ttgtgaagac
    16141 gaagattata gctatttgga ttaagctgac tttcaacaaa gggttcaatg atgatttctt
    16201 ttcctaatct tcttttaatt tctctccccg ataatatcat ttgttttccc ctcttaattc
    16261 ttataaatta ttgcatatct ttacattttt ataataatgt cacaaaaatt tatactatcg
    16321 tataaataat atattaaatt acccgatttg tctaatagta ttttaaataa ttttttcaaa
    16381 aaataaaaat gtcagttcag gactcagctt ttcttcttta aaaactttat agccaagctt
    16441 attatagaga taaatatttt tttcatcttt gaaacctgta aataattcaa atcttgatac
    16501 atccgaatag ctgttttcta tttccttcat taatttttgt ccaattcccc tgttttgata
    16561 actgggatct acaattaatt ttccaatata acaagtatta ttgtttttat aagctctgac
    16621 actaccaatt atttttcctt ctgcttcagc ttttaatatc aatcccctgt caaattcttc
    16681 ttctaattcc tgaagctttg ggttaaaggc tgtatattaa aatcattata tcttatggca
    16741 ttttccttat aacatatgtg ctgtaaatct aatatttctt ttaaatcttc tttatttgca
    16801 atttttattt ctatgttcat tttttacctc cttaaaatta tccacaagat ttttactttt
    16861 taaaagaatt atccacaatt attttataaa tgtggataat tttcataaaa agtattacag
    16921 gttttgactg ctttaataaa agcttttatt tttgactcat ccttttcacc tttgctttct
    16981 acacctgaac tgacatcaac aatatgggga ttgacttctc ttataccctg gactacattt
    17041 tcactattta agccacctgc taaaatcgta aaatatttgc ttgaaattcc tttaatcaaa
    17101 tcccatgaaa aggtctttcc gctacctcct ttaccactgt caaagacaaa accctgaata
    17161 gccttatact cttctaagcg gttaaggact tctttatctt gcacagaaag ggctttccat
    17221 acaggtacaa tagatcttga gcattcatca tccttttcat tcccgtgaag ctgaacaata
    17281 tccagatgac aatacttagc tatttgattg acttcttcaa tgggggtatc tacaaaaacc
    17341 cccaccgctt taatacctct tcttaacttt gaccgcattt ctttcgccag ttcaggtgta
    17401 acctgtcttt tactttctgc aaaaacaaaa cctacataat caatatcata tttattaatc
    17461 atatagattt gttcaacact ttttaaacca cagattttga tttcaggcat tgtaagcatc
    17521 tttaaactcc tttgcctttt tattaatatc ttctgatttc ataaaacttt ctccaactaa
    17581 aatcccgtca atattggcct tttttagctt cataatatct tcaccggtaa aaattccact
    17641 ttcactgata attaatttat cctttggaat ttccctgctt aattttatcg ttgtattgat
    17701 atccacgcta aaatctttta agtttctatt gttaatacct atgataaccg cattggtgtt
    17761 taaagctctt tccagttcat cttcatcatg aacttcaacc agggcatcca tatccagaga
    17821 atgcgtcagg cttataaatt cccttaactg gatatcatct aaaatggctg caataagcag
    17881 gactgcactt gccccaagag cccttgcttc atatatttga ataggatcaa taataaaatc
    17941 ttttctaagc aggggaagct ttacttcccc atgtatttct ttaagatatc ggggagaacc
    18001 taaaaaaaac tcttcttctg ttaaaacaga gatggcatcg acagaagact catattcttt
    18061 tgcaatctct acaggattaa attcttgtct tatgatgccc tttgaggggg aagctttttt
    18121 gacttctcct atgatagaaa ggccaggctt ttccatggct ttataaaaag ataaaggaat
    18181 ttcttttttg tctgttaatc tttcaaaata agcaatactg gccttttttt taagctggtt
    18241 aagggattct ttttttcttt caacgatttt atttaatatc atgatacgct ccttgtatat
    18301 tcaatatagt catttaattt atttaaagca tatcccccgt caataagttc tctggataaa
    18361 tgtatacctt cttcaagact ttttgctttt ttgcctacat agagggcagc accgctgttt
    18421 aaaagtacaa tatctctttt aggtcctttt tctccatcta aaatgtttct tatgatttca
    18481 gcattttcct tcgcatcgcc cccaagaatc tcttctttgc ctgccctttg aattccaaat
    18541 tcttcagggg taatataata agtatggatt tcaccatttt tgacttctgt cacctttgtc
    18601 ttatccgtta cagtaatttc atccaggcca tccatgccat gaaccaccat cgcatgctca
    18661 acacctaaat ttgcaagaac tatacctaaa ggttctgtta acttttcatc aaatacacct
    18721 aaaacctgtg attttgcatt ggcaggatta gtcagagggc ctaaaatatt aaagatagtt
    18781 ctaaaaccaa gttcttttct tacgcctgct gcatgtttca tggctttatg gaaggtgggc
    18841 gcaaataaaa agccaatatt tttatcttta acacatgctt cgatttgctt gggtaagaga
    18901 gctatattgg cccctaaagc ctcaagaaca tctgcactgc cacttttgct ggaaacagaa
    18961 cggtttccgt gtttaacaac atgaattcct gccgctgctg caataaaagc aactgcagtt
    19021 gaaatattaa aggttcctga gcaatctcct cctgttccac aagtatctaa agtataaaag
    19081 ttttctaata taattttttc tgcttttttt ctcatagctt tagcgccgcc agtaatttct
    19141 tccggtgttt cccctttcat tctaagagct gttaagaaac tgccaattag agcaggggaa
    19201 gcttcaccat ccataataaa ttccatagcc tccatcattt cttgttcgga taaatgttta
    19261 ccttctaaaa gattttgaat ggcatatctt aacataatat caactccttt tttaagaaat
    19321 tctcaatgat tttttgacct tccggagtat taatagattc agggttaaat tgaacgccta
    19381 ccaagggata ttctttatga aagattgcca taacttcttt gtcttctgta atagctgcta
    19441 tttcaaaaca atcagaaatc gtatccatat ccaaaaccag agaatgatat ctgccaaccc
    19501 tcataggatt tttaatctct tcaaaaatat tcattccctt atgcatcaca atggaagtct
    19561 ttccatgaga aagcttcttt gactgtatca attttgcccc aaaagcaagg cctatacact
    19621 gatgaccaag acctaccccc agaatgggga tttttttata aaaattcttt atgatatcca
    19681 tacataaacc agctttttga ggcgttccag gtcctgatga aatcacgata tgagaaggat
    19741 tcatcttttc tatttcttct atagtaattt catcattcct tacaacttta atttcttttt
    19801 tatatgctcc aatatattga tataagttat aagtaaaaga gtcgtaatta tcaattaata
    19861 aaaccataat aacctcctta ttctacggct tttagtaaag ccatgatttt attttcactt
    19921 tcttcatctt ctttttcagg gtcagaatcg gcaactaccc ctgcccctgc ttgcatataa
    19981 accttaccgt ctttaatcac cattgtccga atcgcaatgg ccatatccat gtctccgtca
    20041 aaactgatat aacccactgc tccaccgtaa atacctcttt tttcttcttc taattcatca
    20101 atgatttcta tagagcgaat ctttgggaca cccgacaaag tccccgctgg aaggaaagat
    20161 tgaagaattg aaaaggcatc ttctttttca ttttttattc cttctatctg ggattccata
    20221 tgcataacat gggaataata atgaatatcc atataccttg tcagttgtac agaacctatc
    20281 tgggctatct tgcccatatc atttctggct aagtctatca gcataatatg ttctgctttg
    20341 tctttttcat catttaataa ttcagccttt aaggcttcat cttcttcatg atttttacct
    20401 ctttttctcg ttcctccaat tggacaggta taaactctgt ctttccttaa ctctactaag
    20461 gtttcaggag aactgcccgc tacctgataa tccccgaagt ttaaataata aagatatggc
    20521 gaaggattga tccttcttaa ccttctgtaa agtacaaaag catcttcatc agtttccatg
    20581 gtccatcttt ttgaaataat aatttggtag atatcccctt cttccatata cttttgagcc
    20641 tttttaattt tctcaataaa ttcttctttt gtcatattgc ttgatatttt tttgtacttt
    20701 actttatctt ttatagcctc tatttcttct tcctttaaca attcttcctc catctgttgc
    20761 attttttctt ttgctctttt ttctccctca tcactttctt cttctatcac taaaagataa
    20821 atctgttgat gaaaatgatc gtatgcaatc atttcttttg taatcataaa atgtaaatct
    20881 ggtacgccta taacatcttt atttgccggt ggaagctttt ctgcctgtct gattaaatca
    20941 tagctgacag tacctactgc cccgcccata aatggaataa agtcactggg cttaacgttg
    21001 aaagcagaca tatattcctt tacgatatct aaaattttcc cttgcttttt gatgacctta
    21061 tctccttcga tgatggtgac ttccctgtct cttccctgta tctcagcaaa aggtctttta
    21121 gctataaaag aatatctgcc ttttttatta tcccggcttt ctaacaaaaa acctttttct
    21181 ttccctacat atttgttaaa tattgtgatg ggtgtttgag tatctccatc taactttttt
    21241 gaatatagtt tataaatcat attttccctc ctcgaaatat aatgaataga atataaaaaa
    21301 gcccttcgtc ccactattgg gacgaagggc ttaaccttcg ctatgccacc caacgtgttt
    21361 taatgttgct cagctaaata aaaaagacat ttatcccttg ggacaaatgt cttattagcc
    21421 acccaaatca tcaaagcact ataaatccga tacggagtat atactcgata tcttacctct
    21481 gtaacgggag gacccggcat cggatactca aagttcacct agcatctcct gggaccattc
    21541 cttaaattcg tttgtaccgg acctttcacc aaacgccggc tcgctgagac aaactcctaa
    21601 aagtactctt cccattcata gatttttaac ttaactttaa acttttttat attatattca
    21661 aaaaatttgt ctttgtaaat atatttttta tatttcttta aaatattcta cgattcccag
    21721 ataaatagcc catgctatct tttcctgata gatttcatta ttgagattgg cttcttcttc
    21781 gggattggtt aaaaaaccac attctacaat aactgcagga atgtttgtcg tttttaaaac
    21841 atagtaatcc gtattggctt ttgcttctct tttattggtg agatctacaa attcccttaa
    21901 ctgtttttgt attgactttg ccaaacgttc tccttctttt gaatgaccat ggtaaaatac
    21961 ctgcgcccct ttatactttg actcagaaaa gctgttttga tgaatactga ttaaaatatc
    22021 cccttcacta ctgttaatga gttcttttct tcttcgcata tcggaacgtt ttttattatt
    22081 atcaccctct tcattcaaac cttcatcact agttctggtc ataacaacga aggctccgct
    22141 ttgttcaaga taagcttgaa gtttaagggc aatagcaaga ttaatatcct tttcatgggc
    22201 accatgaatg cccagtttcc caggatcaaa cccaccatga ccagggtcta ttacaataac
    22261 ccgcttagcc gttggtaagg caaaagtact gactgtcctg tttgtatata tgttataagc
    22321 aaataataca actaagaaaa gagcaacact tgtacagagc ttcggattct ttttccagaa
    22381 atccatgtcc cacctcatat atcttttacg atgattttta ttttctctat ataattattc
    22441 accgcaaaaa aatatatgcc tgttttattc ttttaaatat ttataaagta ctttccatat
    22501 ttcaggcttt tctaaacctc ttttccagcc ggcagtccct gccagatcgt acttttgaac
    22561 cagcttaagt ttttcttcaa tagacctttc ttcctctaac cagattttgt aaacaacatc
    22621 acccctggtg tattcaccat aatattgacc tgcatcttcc agccacacag gctcaacccc
    22681 tgcatcttta aggaattgtt ctcccctgct cataccataa gctttagaag aaacttttat
    22741 ttctccttct acttcttctt cagcccataa gcgggtataa aaaggaatac ccaggataat
    22801 cttttcatgc ggaacttctt ctaaagtatt tttgattcct tgttctaccc agcctattga
    22861 tgctacagaa ccgctctcag gggatgttga ccagtgttca tcgtatccca tgataataat
    22921 atagtctacc acttttccaa cttccgaacg gttataatgt ctggtccagc cagaaggcac
    22981 atacatatct actgaaacaa caaggccttg ttcttttaac aaaggcgtta attctcttat
    23041 aaactgtaca taatattctc cagtctcctt ctttaaactt tcaaagtcta tattgattcc
    23101 gtccaaatta tatatggatg caaacgccaa aagctgtttg atgatatttt ccctgctgtc
    23161 ggtattactt aataccttat gggttaaatc cgggtctgaa aaaggatttg taatcatagc
    23221 ccatacttga tagccctgac tatgagccca atcaacatat cttttacttg caatattcct
    23281 gatgttacct tgttcatctt caatttcaaa ccaactgggt gacaaaacat caagaccata
    23341 tataggctca aatttagaag gaacttcact gctttctact ttaaaaacct gatcccagac
    23401 taaattaatt ttgccctgta atggctccca ggtagggaaa ctttcttctt tttgaacagc
    23461 ttttccttca atctcttcaa tcgtcccgat gtgcttttta ctaatatagc ctacaatccc
    23521 atgttcattt cttacatagt accaatcgcc agcttcttca aaaatcctga ccttatctga
    23581 taaggacaac ttaactaaaa taggactttt gatatgattt tcataacgga taggggcatt
    23641 ctttttaatg acttcaccaa ttattttagt ttctgttgta tagtccatta caaccatatt
    23701 actttcttta taatagaccg cttcgatgtt aaaccattcc tttaaaaagt taaagggaat
    23761 ataaggtacc tcttcaaatt tccttagagg aacttgaagc tctaaaggtt cctggttaat
    23821 ataataagtc aattcatcgg ttttcatgcg gataacctta ttctgcgtag ttatcgtcaa
    23881 tttttgtgtg ccttcatccc agaaaatata cggatcaatg tacgtcctga caaaatcaaa
    23941 gggcaaaaat atttctccat caatgacttg cggactatgc tccatagcaa tgatttcttt
    24001 tcctaaaacc aggccgactt ttccttcaga aagctctgaa aaagcttcat aggctgaaat
    24061 atagctgaag tttggcatgt atttttttac caatagtaaa gatactgcaa tgattaccat
    24121 aaacagtagg catccaaaag atcttttctt ttttttcact ttaatccccc taaatttttt
    24181 gtctctatat ataaacgtgc aaacttttta ttggtatgct ctcaattata acataaaaaa
    24241 ccacaagttt aataaaaaac ctccagtaaa aataaggata ataataaaca catataaata
    24301 atgtataagc actttttaca acaaaaataa cttgttaaca ttaaaagagc tgttcatcaa
    24361 tatttttgtg gacagctctt tttaacaatg tattcttttc tgatttctta tggattttct
    24421 ataagatatt tttccactgc attaattgca attgccccat ctgctgcagc agtaataatt
    24481 tgtcttaact ttttatctct gatatctcct atggcataaa cccccggaat gtccgtttca
    24541 caattttcat tagctaaaat atagccccat tggtcagttt taaccttacc ttttacaatc
    24601 tctgaattag gtgtcatacc tacggcaata aaagcacctt ctacttccaa tattttttct
    24661 tctcctgatt ccttatcttt tattttaatt cctgtcactt tatcttctcc taatatctct
    24721 aaaacctgtt gattggtaag tacctccaca ttatctaaag aagacagttt ttcctgcaaa
    24781 atcttttgag cagtcaagtg aggaaggtct tgcaccaaat atactttggg tgaaaactta
    24841 gccagcagga tggcatcttc tacacccctg ttgcctccac ctacaactac tgtatttttt
    24901 ccgttaaaga aagcgccatc acaagttgca caataagata cccccctggc ctttaatttc
    24961 ttttctccag gaactccgat ttccctccag ctggccccca tcgccaataa aatcgtcctg
    25021 ctttcgtaag tcttattttt tgtcatcact ttttttattt ttccttctgg ctctatgtca
    25081 ataacctctt caacgattgg ttcaattcca tattctttcg catgagcttg cattttcagg
    25141 gctaattcag gaccaatgac gtctgcaata ccaggataat tagctacttc ataggttttt
    25201 gtaacctgac cgccaacaaa ttctttttca aacatcactg cattcagctt tgctctgccg
    25261 gcataaattg ctgctgccat tcctgcagga cctgctccaa taataatgac atcatacata
    25321 taatcgcccc tttacatata tattttatct attataatca ttataataat gatggtaaaa
    25381 taaaaacaat tgttatcgaa ttctaattgg taacatatga tttaatatac aaatattttc
    25441 agttatttgt ttacataagc caatatgtga cattattccc ctctttacat tttaccctga
    25501 tatagattat actatacgta tatatctata agataatatt agattggaag tgatgcttgt
    25561 gagaattgaa aaaataagcg acactcaaat aaagattatt ttgaatcaag cagatcttaa
    25621 aaatagagac attaaaatca gtgaattagc ttacggttct aaaaaggcac aagaattatt
    25681 ccgggatatg atggaaactg ccttagagga acatgatttt gatacccata atgttccttt
    25741 aatgattgag gctgttcccc tttctgtaga cagcattatg attattgtaa ccaaggtgga
    25801 acaccccttt gaaattggcg aaaagattac acctttccgc tcaaaatcca acacccggac
    25861 ctttaagaag aaaacttttg tcccagaaga agaggaagat tcaactttaa tgatttattc
    25921 ttttgagaaa ctggacgatg ttgttgaagc ttccaaccgc atctaccacc tgtactgcgg
    25981 ggcaaattct ctttataaag atgtttacaa taacagatat tttcttgtcc ttcacaaaaa
    26041 ctactttgta gataccaatc tcaatcaaat agaatctatt ttaagcgaat atggaaacaa
    26101 acatatctca acgcctttaa cggaaggcta tttagctgaa cacggagaaa taatgattca
    26161 aaataaagcc attgaagttc ttaaaaaaca tctcgcataa aaaaactgac gttatcgtca
    26221 gtttttttct ttttctgcta agtatgcatt cattttttct atcacttcat ctacattgag
    26281 tccatgaaca aaaccagctt cttctaaagt ttctccctga gatgaaggac atccgataca
    26341 atgcattcca gcttccatta aaataggtat cagccctcta tctatagcaa taatatcagc
    26401 aattatcata tcctttgtta ctttagctgc catctttaat cctcctttat tcataaaatc
    26461 ttgttacaat tttattatat cctaattata caatataatg caacaaccaa attctactga
    26521 actgcattca tcagccttgg aacaacttgc tttttccttg ataatacacc aggcagaaag
    26581 atactgtcct ctttcagacc cataccaaaa gctttatgag ccagttcttt tgcaggtcca
    26641 attgcaataa gttctgtccc gccaagaata atatccgtta ccattaaaag agccaagtcg
    26701 tattttttat cttcacatag ttttttcatt atttctttta tatccgttat caaatcaaat
    26761 attcctttaa agtctgctgt attaatttga gaaataacaa tattgtagtg gccaaaaaca
    26821 aagtttttca tatcggaata atagatttct tctgccgtct tgccttcaag ggaagtacct
    26881 gcagcaatta acttcatgcc ataagatttg attttaaccc ctgcaatttt tgcaagatat
    26941 tctgcttcct ttttatcctc ttctgtacaa gtgggagact taaagataag cgtatccgat
    27001 aagattgcac ttagcataat accagccata ctttttgtgg gcgtaatatt attctcttta
    27061 tacattttag atataatcgt tgccgtacta cccacaggtt ctgcccgaaa atataaagga
    27121 gacatagtat aaatatctgc taccctgtgg tggtcaataa tttctaagat attagctttt
    27181 tctaaacctt ttatcgattg tcccttttca ttatggtcaa ctaaaataac atttttgcgg
    27241 ttaatatcga ttaaatttct tcttgaaata acccctttca caacaccttc tgaatccact
    27301 acaggaaaat tcctgtagac agaactctgc ataatttctt ttacttcatc aatatagtca
    27361 tccatctgaa aatagactaa atttttcttt ttcataatcg attttacagg aatactttga
    27421 ttaattaatt taattgtctt aaaaaaagta tgtggtacag taacaacggc acaagttaat
    27481 tcttcttcac tgtcaaaaag tttcttactg gttttacctt ctgcaacaat aatgcagcca
    27541 gcacctgatt ttatcaccct tttgtggagt tttttaacgt ggccggtaat aacgatatca
    27601 ccctttgtaa gaatttgatc ttcatctaat gtagaatctg tataaatatt tccctccaca
    27661 tattcatatg gataactgcc cgcaataacc tctccatcta aagcctcaat taatttagaa
    27721 aacttcgctc tttggttttt cagggccatt tcatcttcca attccatata ggcggttgca
    27781 atatcggtaa tggataccac gccagtcaat ctattcttct catctacaac cgggaacatc
    27841 tttcttttat tatttttcat caattcccat accgttttta cagaatcatc tttacgcacc
    27901 agttcagaca cataaaaatt taaatcacaa acttgaggtt ctacattttc taataacaca
    27961 ggaatttcca catcaaaata atccagcaca aacttcgttt ctctgtttat atcacccagt
    28021 ctggctgcaa caacattttt ataacctaat tcttttttca gattggtata agcaatagct
    28081 gaacatatag agtctgtatc cggattcata tgtccaaaaa caaaaatttg tttatccata
    28141 ttaattgacc ttctatcctt cctttaaatt aatgtttaag ataagttatt ttgtttaatg
    28201 cttttattgt tttattattt tatcacactt attacaagtt tcaaacttat tctctattta
    28261 tatatatttt tctttttgca catactaaat aacgtataaa aattttaatc ctttaggaat
    28321 ttttattatt taatgaacaa tcctattata aactgtcatc tttaaatcta ttcttagata
    28381 aatatataat cataaattgt taaatattta gctttttaaa ctaatcgtgt ttactctgta
    28441 agaagcggat atttgaaaat ctttttcaat tgtttttctt tttcatttta ctgttttgtc
    28501 atgaattcct ctgtttccaa taattttatt aagaattatt atcattatta tttaaaaact
    28561 tattgacgtg tatacagtat tcatatataa taaaatcaaa gataatcatt ttatatagtt
    28621 tctgttaata atataacaac tcatatagat tttaaggagg taatctaatg tttgaacaat
    28681 ggagaaattt tagagaagga aattgggtaa aagaaatcga tgtaagagat tttatccaaa
    28741 aaaactatgc tccttacgat ggtgatgaca gctttttagc cggaccaaca aaaaggactt
    28801 taaaactttg ggataaagta atggaactga caaaagttga aagagaaaaa ggtatcctgg
    28861 atgctgaaac aaaaatacct tccggtatta cttctcatgg acctggatat ctggacaaag
    28921 aattagagtt aattgtgggc ttccagacag ataaaccttt aaaaagagga atcatgcctt
    28981 ttggtggcat ccgtgtggta gaaaaagctt taaatgctta tggctatgaa ttagatcctg
    29041 ccacaaaaga aatttttaaa tcgtacagaa aaactcataa cgatggcgtc tttgatgctt
    29101 atacggaaga aatgagaaaa gccagacatt ctggcattat caccggtctt cctgatgctt
    29161 atggaagagg cagaattatc ggtgattaca gaagagttgc tttatacggt gtagacaagc
    29221 tcattgaaga aaaacaaaaa gaaaaaagac aattagccct ggaacacatg gaaagtggtg
    29281 ttatccgtct tagagaagaa ctttcagaac aaattaaagc tttacatgaa ttaaaggaaa
    29341 tggcacaaag ttatggcttt gatatcagtc agcctgcttc taatgcaaaa gaagctatcc
    29401 aatggactta ctttgcctac ctggctgcta ttaaagaaca agatggggca gccatgtcca
    29461 tcggaagagt atctactttc tttgatatct acattgaaag agatttaaaa gaaggtattt
    29521 taactgaaga agaagctcag gaattaattg acgattttgt tatgaaatta agaatggtcc
    29581 gcttccttag aacccctgct tataacgaat tgttttctgg tgaccccaca tgggtaacag
    29641 aggctattgg tggcatgggc ttagacggca gaaccctggt taccaaaaca agttttagaa
    29701 ttcttcatac tttaaataat ctaggtcctg cacctgaacc aaacttaacc gtattatggt
    29761 ctaatcatct gcctgaaaac tttaaaaagt tctgtgccaa aacttctatt aataccagtt
    29821 cgattcaata tgaaaacgat gacttaatga gaagatggta tggggatgat tatggcattg
    29881 cttgctgtgt atctgccatg agaattggta aacaaatgca attctttggt gcccgtgcta
    29941 atctggcaaa agctctctta tatgcgatta atggtggtaa agatgaaatc agtggtgaac
    30001 aggttgctcc cccatttgct cccattacat ctgaatatct ggactatgac gaagtaatgt
    30061 ccaaatttga tcagatttta gactggcttt ctcttttata cattaatacc ttaaatatta
    30121 ttcattacat gcatgacaaa tatgcttacg aaagacttca aatggctctc cacgatatgg
    30181 atattctcag aacctctgcc tgcggaattg ctggtctttc tgtagtcgca gactccttat
    30241 ctgccattaa atacagtaaa gttaaagtga taagaaatga acaaggctta gcggttgact
    30301 atgaagtaga aggagaatat cctgcctttg gaaacaacga tgacagagtg gattccattg
    30361 cttattcgat tgttaaaaac ttcatgaata aattaagaaa gcataagact tatcgtgatt
    30421 ccatcccaac cttgtccatc ctgacgatta cctccaacgt ggtatatggt aagaaaacag
    30481 gaagtactcc tgatggaaga aaggctggag aaccctttgc acctggcgca aatcccatgc
    30541 atggcaggga taaaaatgga gcgatcgctt ctatggcatc tgttgcaaaa cttccatacc
    30601 agcatgcaga agacggtatt tcctatacct tctctatcat tccaaaagct ttaggtaaaa
    30661 atttagatga aagaatcaat aacctggttg gcttacttga tggttacttc catgatgatg
    30721 gtcatcacat taatgtcaat gtatttgaca aagaaacctt attggatgct atggatcatc
    30781 cagaaaaata tcctcaatta actattcgtg tttctggcta tgcagtaaac tttataaaac
    30841 ttacaagaga acaacaatta gatgttatta atagaacttt ccatgaaagg ttttaaaaaa
    30901 tgatgaatta tgataatatt ttgattgaaa agaaaaagct ctccccaatt taccccactg
    30961 aaaggaggcc tataatgagt gtaactggta gaatccattc tatagaaact tgtggtactg
    31021 tcgatgggcc gggaataaga tttgttatct ttacccaggg ttgtcctctt cgctgcaaat
    31081 actgtcataa tccggatacc tggaaaatct ccgaaggtaa aaacatgact gtagatgaac
    31141 tcatccagga aattgaaaag tataaatctt atatggactt ttcaggcgga ggtgtgacag
    31201 taactggtgg cgagcctctt atgcagccta aattcgtaaa agaattgttc cgccaatgca
    31261 aggaaagagg aattcataca gctttggata cctctggtta cattcccctt gaacacgcaa
    31321 aagaggtcat ccaatatact gatctcattt tattagatat taaatctttt gactatcaga
    31381 cttataaaga attaacaggt gtttccaacg aacctacctt agaattggct aaatatcttt
    31441 cagacattgg taaaccgatg tggatacgct atgtccttgt tccaaattta acggacaatc
    31501 ctgaccatat cgaagcttta gcccaattca taaaggactt gaaaacagtc gaaaaaatag
    31561 aaattctccc ctttcataaa atgggtgaat acaagtggga agagctgggt tataattatg
    31621 aacttaaaga aacccctgct ccttcaaaag aattagtgga ctcagtaaaa gaaattttta
    31681 aaaaatataa tctaaaagta taaaataaat cttctcataa aaaggaactg tacacaaata
    31741 aattgtgcac agttcctttt gcaataataa aagtaaagag ttgttcgtta acagataaat
    31801 aattgttaaa gcacaaccct ttttattgaa ctattataaa attttccact acatattcat
    31861 tggatcttga gaattatatt gttgtgaatt agaagaaaca tcttcgttag cagctctgat
    31921 ggcttccttg tctcttaaag taaataagaa aataggtgta aagagggtcc agataattat
    31981 cattggaata ctaacttctt catgatacat tttatataat gtataaacaa cacatgcact
    32041 aaatattata ttaagaggtg ttgcaataat tttaattact ggcattacac taataccaaa
    32101 aacagctaat tgagctaatg gaagcacgac ttccgtatta ttaatttcaa ttttgttaat
    32161 cttagctgga cctattactt tacctagtat ataatagttt gcaataggaa tccaagcaag
    32221 ccaagcattt tctatatcct ttttcttggc cattccatat attcctaccg cagtaagaac
    32281 atatgtagct acaattattg cgaatacaac tagtg
//
LOCUS       DAOSUD010000017        30387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1023_length_30387_cov_22.722372, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000017 DAOSUD010000000
VERSION     DAOSUD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 30387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30387
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_30387_cov_22.722372"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..663
                     /locus_tag="PLG49_03790"
     CDS             22..663
                     /locus_tag="PLG49_03790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOQ16472.1"
                     /translation="MKNIFSPGVKVEIKEYGTDDNDSYCYTSQIEEVLKKDVLVLGTP
                     IVGGQIVPLRINRIYILYIYSSNGIYSCKVRPVRHYKKGLIELVQVEIISDLAKIQRR
                     EFFRHECVQPFKFRINENWFDGVIKDISGGGIRFISNDELEVKDHIYCRIPIENDSIE
                     IEGIILFKEKANVELYKYQYRVKFINVVKTDQDKIVQYIFEQQRKRVRQEKGL"
     gene            663..1736
                     /locus_tag="PLG49_03795"
     CDS             663..1736
                     /locus_tag="PLG49_03795"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOQ16473.1"
                     /translation="MIVDRKIKILVVDDSAFMRRVISDIINSDNRFTVVGTAKNGEEG
                     LEKVRQLDPDVITLDVDMPEMDGLEMLKNLMKENPKPVLLISAYTKEGADTTIKALGL
                     GAADFITKPSNIFKMQDKEIRDNIIKRILLISNISNFPSDNKRQLKETGHNKQKEQVH
                     SVKIKKIVAIGTSTGGPRALQEVLPELPEHLPATYVIVQHMPAGFTKSLAERLNNLCQ
                     IKVKEAEDKDILHPGVAYIAPGNYHILVEKMPYSNDLWIRLSSSPSVSGHRPSVNVML
                     NSLSELNIDNIVGVIMTGMGNDGCEGMKKLKLNNKAYVIAQDEKTSVIYGMPKAVIDE
                     GIADAVVPINQIAKEIIRVVEVL"
     gene            1738..3840
                     /locus_tag="PLG49_03800"
     CDS             1738..3840
                     /locus_tag="PLG49_03800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOQ16474.1"
                     /translation="MDMSQYLEIFIEESKEHLQSLNESLLQLEHEPGNMPILNEVFRV
                     AHTLKGMSGTMGYTKMQNLTHNMENVLSEIRNGNIVADSNLLDVLFKCLDALESYVDE
                     ITNSGKEGNEEYKDIIDELTNILSGKSKKVDNNVAVASSPEIKAPVTEDNITNNITDD
                     SGDAIDVKEAALTDFEINAVREAFARNYNVYQIHIFLNHGCVLKSARAFIVFRALETH
                     GDIIKSIPKVEDIEDEKFEFDFTVVVISKDTKEDFEKDLLSIAEVDQVIIKNVLLSDK
                     IELAAEDVKEVHNTPEFDRQSNVSRSMADDESDDNDSSGKAKRKAGKTVRVDIDRLDT
                     LMNLVSELIIIKTRLEGIERDKTNQNYNEAIEYLERITTSLHDAVMKVRMVPVERVFN
                     RFPRMIRDLSRKLDKDIELIMSGEDTELDRTVIDEIGDPLIHLLRNAADHGLETKEER
                     LHSGKPEIGHIYLRAYQDGNNVVIEVEDDGRGINVNKIKQKAIEKGTISYEAAANMSD
                     QDIIDLLFKPSFSTSEKISDISGRGVGLDVVKTKIEALGGDIEVKSELGKGSKFIVRL
                     PLTLAIIQALMVRLGNEKYAIPLNTIQNIEDINVSDIQLVQQQEVILLRNQVIPIIYL
                     EDILDVPKNEQCNTITVVIVKKGEKQVGFVVDGLIGQQEIVIKSLGKYLSGIKMIAGA
                     TILGDGEVALILDINTLV"
     gene            3855..4331
                     /locus_tag="PLG49_03805"
     CDS             3855..4331
                     /locus_tag="PLG49_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOQ16475.1"
                     /translation="MEGISNQEVRQFIVFKLGNEEYGIDILNVQNIERVLSITRVPKA
                     PFFIKGVINLRGEIIPVMSLRLKFNLEADEYTDDTRIIIVKIDDNQIGMIVDEVKEVL
                     QLTADSIESVQGVSGINTDINLSFIQGVGKVNNHIVTLLNIKSLIDSILVDSLENA"
     gene            4450..5067
                     /locus_tag="PLG49_03810"
     CDS             4450..5067
                     /locus_tag="PLG49_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOQ16476.1"
                     /translation="MVDIDKLNHLHLDILKEIGNIGAGNATTALSKLINKKVDMKVPK
                     VNILEFKNLADILGGPENPVVGVLFDVSGEIEGMMMFVLEQRFAHTLVNILMERDIHH
                     ISEFSEMDISALNEIGNILASSYLSSLSALTRLMIVPSIPHLAFDMAGAILSVPAIEF
                     GKVGDRVLLIETEFAEGIDRVVGYFILIPDIKSFKKILSSLGVSV"
     gene            5067..5558
                     /locus_tag="PLG49_03815"
     CDS             5067..5558
                     /locus_tag="PLG49_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOQ16477.1"
                     /translation="MSSQEIIKVGMADLNTAVYPDILTTLGLGSCVGIALYDPVARIG
                     GLAHIMLPDSTQIKNNSNKAKFADTATIILVEEMIKLGAVKERIVAKLAGGAQMFAFN
                     NANELMRIGQRNVAAAHSILEKLGIKVIASDTGENYGRTIELYTEDGRLVIKTIGHGI
                     KEI"
     gene            5567..5866
                     /locus_tag="PLG49_03820"
     CDS             5567..5866
                     /locus_tag="PLG49_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16478.1"
                     /translation="MNKKLDIILPLISAIIILSIGIIKKTAYITIIKELIIIIIIFYF
                     IGNIFKGLINNIYSESDKNDHSQITAKADANLSLNDNISTNSESEGNIQEEAGTQ"
     gene            5937..6704
                     /locus_tag="PLG49_03825"
     CDS             5937..6704
                     /locus_tag="PLG49_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOQ16479.1"
                     /translation="MSKKSLEMLWEQYEATKDVSIKDQLIMEYAPLVKYVAGRLNIYL
                     GQSVEYEDLVSYGVFGLIDAIEKFDFKKGVKFETYASLRIRGSIIDNIRKLDWVPRSL
                     RQKNKEIEKTIMELETELGRTATDEELAQKMNISIEELHEIYKKTNLISLISLDEYIE
                     QNNDYNISSEKSPATEQPESYIEKQELKRILKESIEKLPEREQKILFLYYFEELTLKE
                     ISSIMGVSESRISQLHTKAITRLKTKLGKYKSILYEL"
     gene            6719..8362
                     /locus_tag="PLG49_03830"
     CDS             6719..8362
                     /locus_tag="PLG49_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOQ16480.1"
                     /translation="MYNPDKFTRSGYPNFDGFFDFELREDGLYLILHGPMGKGKPVEL
                     DEILSRLRQKNITNYDYAKIKEAVKNIKDSEKLEIYISDQQELEVIDEEVVVNISKDK
                     MFAVISFIPPENNGRLLSYEEVMHAIESAGVNFGIDENEVKRALEERKPNYKYVIAKG
                     LKPVEGTHGKLEFHFNVNKDLKPKVLEDGSVDFRNLDIINNVSKGQVLVTLIPAQPGT
                     PGMDVLGREIFPPKVKNPTLPKGKNTEISEDGLSLIATIDGQAIYINNKVTVNQTYEV
                     PDNVDNSVGNIDFVGNVIVKGNILTGFSIKAGGNVEVYGVVEGACIEAQGDIILYRGI
                     QGMSRGILKSMGNVVARYIENSTVEAMGNVTSEAIMHSRVKCGGSVIVEGKKGLIVGG
                     IIRAGKEVNAKVIGSHMATSTEIEVGIDPTIVERYRYLKDEQANIKKEIIKTNQAIDL
                     LNKMKGTNRLTDDKRALLQKSIRTKVFLNDRLKSIKNEMADLEPLMEERDDGKVRAFN
                     VIHPGVKVTIGTATMYVREEIKYCTLYKDNADIRTTSYS"
     gene            8374..8682
                     /locus_tag="PLG49_03835"
     CDS             8374..8682
                     /locus_tag="PLG49_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16481.1"
                     /translation="MSIRPVDMQVIIPKTTEISKQNQEQTYKGEIQQQEFAQQMEKKI
                     MQHQQQVVNTNKSEYAVNDEGRNKNQNQGNQAKKKKNKNEKDKKSNISDSNSIIDIRI
                     "
     gene            8697..9206
                     /locus_tag="PLG49_03840"
     CDS             8697..9206
                     /locus_tag="PLG49_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ16482.1"
                     /translation="MLQSEILNIMIFFVISIGMIILIIGLVFIKEKNQGEHSSNKNEN
                     FEKELESQINEADKMLQELNHFSSYIKEELDNKYKELLFIYQLIDEKGKELSNDKIQL
                     VSEKNDKNSSDKDNNAHDLEALQILNNKNYDQIIKLYKEGNDISSIAKKLNIGKGEVQ
                     LILGLAKMR"
     gene            9213..9707
                     /locus_tag="PLG49_03845"
     CDS             9213..9707
                     /locus_tag="PLG49_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16483.1"
                     /translation="MGLSTRSFMIGLGLGIVITGITFCFIPYKSISDDEIIQKSKELG
                     MVNPLDLSPEKFVLSQEEIIRRAKKLGMDFVKKDDNLNEKDESHEYYELDVVTIFIPQ
                     GLDSKEISSLLYENHLIDDRDAFDSYLRNNNLDRIIAYGYFDIPTKSDYKTIADIITR
                     RSHD"
     gene            9900..10670
                     /gene="rpsB"
                     /locus_tag="PLG49_03850"
     CDS             9900..10670
                     /gene="rpsB"
                     /locus_tag="PLG49_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOQ16484.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTVKKIEEAYQVIRDVVEEGGNVLFVGTKKQAQDSIKEEAERCGMFYVNQRWLGGMLT
                     NFRTIRSRIERLKELERMEEDGTFDVLPKKEVIKLRHEKEKLEKNLGGIKEMNSLPDL
                     IFVVDPRKEKIAIQEAHILGIPVVAIVDTNCDPEEVDYVIPGNDDAIRAVKLIVATMA
                     NAVLEAKQGVQTSVADEKVEAEEVVAENEVEEAVEQVE"
     gene            10841..11761
                     /gene="tsf"
                     /locus_tag="PLG49_03855"
     CDS             10841..11761
                     /gene="tsf"
                     /locus_tag="PLG49_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOQ16485.1"
                     /translation="MAITANMIKELREMTGAGMMDCKKALTEADGNMEKAVEILREKG
                     LAKAAKKAGRIAAEGLVATYVSEDGKTAALVEVNSETDFVAKNELFKTFVDETAKQAV
                     LTKTDNIEDFMKEAWHLDNSKTVQDVLTEKISVIGENLNIRRFVKYEIESGIIATYIH
                     GGGRIGVMVHLDCNSSSDKLAEAGKNIAMQIAAVYPKYITIDEVPADYVEKEREILKQ
                     QALNEGKPANIVEKMIEGRLKKSLKEICLMDQAYIRDPEMTVSQYLDSVSKEIGDKVT
                     IKAFVRYETGEGIEKKEENFAEEVAKQMNQ"
     gene            11937..12641
                     /gene="pyrH"
                     /locus_tag="PLG49_03860"
     CDS             11937..12641
                     /gene="pyrH"
                     /locus_tag="PLG49_03860"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ16486.1"
                     /translation="MSSYKRVLIKLSGEALSGTKGYGFDYDIVNSVVKQLKTVVEQGT
                     ELAIVIGGGNFWRGRTDNKMDRTKSDQIGMLATVMNSLYVAEVCRANGLNAVVQTPFQ
                     IGTITELFSKDSALEHMKNKTVVFFAGGTGHPYFSTDTGAALRGCEIEADVLLFAKNI
                     DGVYDSDPKFNPNAKKFTELTGKDIIEKQLKVIDTAAASLCMDQHLDILIFPLNFENS
                     IIDVISGKNIGTIIKN"
     gene            12665..13219
                     /gene="frr"
                     /locus_tag="PLG49_03865"
     CDS             12665..13219
                     /gene="frr"
                     /locus_tag="PLG49_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ16487.1"
                     /translation="MDKQYEQFETKMQKSINALEKELNMIRAGRANPHILDRIFINYY
                     GVQTPVNQVANITVPEARMIQIQPWDASVIKDIEKAILASDIGITPSNDGKVIRLVFP
                     ELTEERRKELTKEVKKKGEDAKVAIRNIRREALDHFRKAEKEGEISEDQLKEIEEDIQ
                     KMTDKYIEKIDVHIENKNKDILSI"
     gene            13324..14058
                     /locus_tag="PLG49_03870"
     CDS             13324..14058
                     /locus_tag="PLG49_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOQ16488.1"
                     /translation="MDNHNYKNKLKMDSLPKHIAIIMDGNGRWAKQKQQSRSFGHKAG
                     SKALERIVKAADSLGIAHLTVYAFSTENWKRPKEEVNALMKLLSQYLNQYLKDTKDNN
                     VKIDVIGDITKFDEEIQRKIEKIQELTLKKNGLNLHIALNYGSRDEMTRAVKKIVTDV
                     MESKIDLNSIDDSLISSYLDTKEIPDPDLLIRTSGEKRISNFLLWQIAYTEFYFTDKL
                     WPDFDENDLYKAIFEYQNRERRFGTI"
     gene            14076..14885
                     /locus_tag="PLG49_03875"
     CDS             14076..14885
                     /locus_tag="PLG49_03875"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ16489.1"
                     /translation="MGIRILTALLGIPLLIFVILSGGLILKIALCVFVLIGMYEFYKA
                     MDNKFNPIKPLGYIFALFYILFISSGQQFAFPFMILFLIILLLATVFLHKKYKVVDVA
                     ITFFGFFYVCVLFSHIVQINQLLHGELFIWLIFISAWGSDTAAYFTGMFLGRHKLCPS
                     LSPKKTIEGAIGGFIGSVLLSLVYGIIIQSGLNVSMNHLLLICIVVGGLGSIASQLGD
                     LAASSIKRYVEIKDYGYLFPGHGGVLDRFDSILFTAPLVYYILLLFNFIIG"
     gene            14955..16082
                     /locus_tag="PLG49_03880"
     CDS             14955..16082
                     /locus_tag="PLG49_03880"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOQ16490.1"
                     /translation="MKAISILGSTGSIGTQTLDVVRNLGNIEVKALSTNKNIDLLEKQ
                     IEEFHPKKVAVMDDEKAYELKKRIGSKIEVLKGLEGLCEIAEMDETDTVVTSVVGVIG
                     LIPTFKAIQKKKNIALANKETLVTAGKIIMEEAKKNNVSIYPVDSEHSAIFQCLQGNA
                     HNKISRIILTASGGPFRGKSLKELEMVTVEEALKHPNWEMGAKITIDSATMMNKGLEV
                     IEAKWLFDVELNQIEVLVHPQSIIHSMVEFEDGSIMAQMGEPDMRLPIQYALTYPKRI
                     TNNWPRINFRQRNMLSFEEVDLSVFKCLQLAYDALKIGGTMPAVLNAANEVAVDKFLK
                     KEINFLDIAKIIEATMEKHELIKNPSLSDILDVDQWARKIC"
     gene            16096..17127
                     /gene="rseP"
                     /locus_tag="PLG49_03885"
     CDS             16096..17127
                     /gene="rseP"
                     /locus_tag="PLG49_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOQ16491.1"
                     /translation="MDVLTIFISILVFGLIILVHEFGHFIVAKKSGIMVEEFAMGMGP
                     KLISKKIGETVYSIRAFPIGGFCNMLGADTATDDSRSFTNKPVRTRIAVIVAGPVMNL
                     LLAYIIIVWSVASGGYLSTTVNRVLPNSAAEEIGLQAGDKIISLDGQRIHLKQEIDMI
                     VQQTKGSPLQIVIERDGSRITKLVTPRQEGDRWLIGFEPKIGENNVFNVLSQAFWQVI
                     FIIKQVIIGLIQLVTLKAKGQVAGPLGVMDIIGEAYQEGMKASFYVAIQYLLSITALI
                     SANLGVMNLLPFPALDGGRLIFLFLEGLRGKPVDVEKEGLIHFIGFAVLMTLMVVVLF
                     NDISRIFIH"
     gene            17146..18201
                     /gene="ispG"
                     /locus_tag="PLG49_03890"
                     /gene_synonym="gcpE"
     CDS             17146..18201
                     /gene="ispG"
                     /locus_tag="PLG49_03890"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOQ16492.1"
                     /translation="MINIKRKKTRTIFIGNLPIGSEHPIAIQSMCNTDTRNVEETVKQ
                     IHLLEEAGCEIIRVAVPDMEAANAIAAIKNQIHIPLVADIHFNYRLALTAIEKGVDKL
                     RINPGNIGSRERIKEVVKAAKERNIPIRIGVNAGSLEKDLLDKYGVQPQALVESALRH
                     IRILEELDYYNIVVSIKASSIPLSLMAYEALSEQIDYPLHVGITEAGTIKSGRMKSAV
                     GIGAILSRGIGDTIRVSLTGDPVEEVLCAKEILQALELRKFGVELISCPTCGRTEIDL
                     ISLANEVEKRCANINKNIKIAVMGCVVNGPGEAREADIGIAGGKGVGLIFKKGEIIKK
                     VPENQLLEALFEEIDKL"
     gene            18238..22578
                     /locus_tag="PLG49_03895"
     CDS             18238..22578
                     /locus_tag="PLG49_03895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOQ16493.1"
                     /translation="MLQNEKNHNFLDVFPQLHVDAPIKEMLKDAKIKKINVYKEDKAI
                     EILLDVKNIIYREILEQLSRQIQKQFKGINKVYLKSKYISDDDLLNNKDSKSKVIHLY
                     WDSILHSINDKHPIGTGILKDCHWKLKDNYLVLKVSNNQSSYLRKNELDKKIKWMLNE
                     ELGLNMEVVFEEEMITKDKKKIYEDNLSNIEHDIVENLLATSTTQVINTEEVKDHMPD
                     KETPDPVILGKDINSEAIRIKDITEESDEVVIQGDILSIEYREINHSKYIVTFDITDY
                     EDSITVKFFVKKNIFENAIKNRLKEKCHVWVKGLIQYDNFSKEIVLMAKDIVEKTSKL
                     IRIDQAQEKRIELHAHTQMSSMDGIVSVTALIERAAKWGHPAIAITDHGVVQAYPDAA
                     SASKKYGIKVIYGIEAYLVDDLNAVIQNPQNQTLEDEYTVFDIETTGLSPTKDKITEI
                     GAIKIKNGKIIDYFSCFVNPEMPIPANIQELTGITDEMVKDAATIEHVLPDFLKFIGD
                     SVLVAHNANFDVSFIRTAAENMSLSVQNTVLDTLELSRLLFPSLSRHKLDIVAKHLGV
                     SLENHHRAVDDAKATAEIFIKCIDILKEKNIQNLNEINALAGAEIDVKKLKTYHAVIL
                     VKNQTGLINLYKLVSFSHLEYFNKRPRIPKSLYLKYKEGLMIGTACEAGELYRAILNK
                     KSDEEIKRIASFYDYLEIQPLDNNQFLIDNGTVESVDELKKINMNIVELGTLLNKPVV
                     ATCDVHFLDPQDEVFRRIIMAGQGFSDADNQAPLYLRTTEEMLEEFSYLGEKKAREVV
                     IDNPNKINQFIENIKPIPDETFPPKIEGAEEELTRITMKKAKSIYGDPLPPAVQNRLD
                     RELNAIIKNGFAVMYIIAQKLVWKSLEDGYLVGSRGSVGSSFVATMASITEVNPLPPH
                     YICPNCKYSDFDSEMVKSFAGGSGCDMPDKICPKCGEMMNKDGHDIPFETFLGFDGDK
                     EPDIDLNFSGEYQPRAHSYTEELFGKDYVYRAGTIGTLAEKTAYGFVKKYMESKNIVL
                     RKAEVNRLVKGCTGIKRTTGQHPGGLMIVPKDKEIYEFCPVQRPANDVNSPVITTHFD
                     YHSISGRLLKLDILGHDDPTVIRMLEDLTGVNPRKIPLDDENTMSLFSSTKALNVSPE
                     QINCEVGTLGLPEFGTKFVRQMLIDTKPKTFSELIRISGLSHGTDVWLNNAQDLIRDG
                     TASLSEVICTRDDIMVYLINKGVEKKHAFKIMEKVRKGKGLEPDDEEAMKAAGVPQWY
                     IDSCKKIKYMFPKAHAAAYVMMAFRIAYFKVYYPEAFYATYFTVRAEDFDYCLMCKGE
                     NKVRKAIKEYEEKGNNLTTKEKGTLTVLEIVLEMYARGFKFIPIDLYKSDAVTFKLTP
                     EGILPPLNALQGLGNTAAFNIVKAREEGEFLSVEDFVERAKISRPVVEIMRENEILTG
                     IPESSQLSLF"
     gene            22824..23288
                     /gene="rimP"
                     /locus_tag="PLG49_03900"
     CDS             22824..23288
                     /gene="rimP"
                     /locus_tag="PLG49_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOQ16494.1"
                     /translation="MSKQNIEQTVETYLKPILDEHNFELVDVEYVKEGTNWYLRVFID
                     KEGGITIDDCELVSKALEKILDKEDPIKNSYILEVSSPGLDRPLKKDSDFEKYKGRIV
                     DIKLYKPFNKKKEYSGELLELRDNIVYIIDEEGNQLSFNRNDIAIVRLAVIF"
     gene            23313..24413
                     /gene="nusA"
                     /locus_tag="PLG49_03905"
     CDS             23313..24413
                     /gene="nusA"
                     /locus_tag="PLG49_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOQ16495.1"
                     /translation="MNSEFIGAIEQIAKEKGIDKELLFEAIETSLVSACKKNFGTSQN
                     IKVSIDRENGDVNVYAQKTVVENVENSALEISLEEAREIDINYELGDIVDIEVTPKNF
                     GRIAAQTAKQVVIQRIREAEREIIYNHYITKERDIMTGIIQRKERKNIMINLGKAEAI
                     LQPNEQIPTEEYELNSRIKVYILEVKQTTKGSQIFVSRTHPELVKRLFEQEVPEVYEG
                     IVEIKSIAREAGSRTKMAVHSNDPEVDPVGACVGQNGQRVNVIVNELRGEKIDIIQWS
                     DDPKEFIAAALSPSKVIKVITDEENKSASVIVPDYQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSESQAKDAGMVLEESSDQDDEANSGIEE"
     gene            24418..24687
                     /locus_tag="PLG49_03910"
     CDS             24418..24687
                     /locus_tag="PLG49_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOQ16496.1"
                     /translation="MKKRKIPLRKCTGCQEMKNKKELIRIVRNQEGEISLDFTGKKPG
                     RGAYICPNEECLEKAQKSKGLERSFKTAIPDEIYEELKSELKKAK"
     gene            24684..24995
                     /locus_tag="PLG49_03915"
     CDS             24684..24995
                     /locus_tag="PLG49_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOQ16497.1"
                     /translation="MSNSIYSMLGLCMKSGNLLSGEFQCEKAVKARKAKLVIIAKDAS
                     YNTKKLFKDKCSYRKIPFYEFGTKEELGRAIGKSPRSSLAVMDENFSAQLIKMFDSCK
                     L"
     gene            25014..27140
                     /gene="infB"
                     /locus_tag="PLG49_03920"
     CDS             25014..27140
                     /gene="infB"
                     /locus_tag="PLG49_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ16498.1"
                     /translation="MSKVRVYEIAKKLNISSKELIEVLEDFGIKVHSHMSSLEDEEAQ
                     LIMEYYSTDKDNDIRDNKKSSDSKDKKKKTKLKDTKTEFGEELHQRTDKMNEQKSTKK
                     DKAFKEKEKEVKSDKKDKEEDTIKIIKISDKITVQSLAQKLDCPATELIKKLMMKGIM
                     ASINQEIDFDTASMIAEEYNTLVEEEKEEDFLESLFAEEADSEENLVERPPVVVVMGH
                     VDHGKTSLLDALRKTNVTATEMGGITQHIGASVIEVNGKKITFLDTPGHEAFTAMRMR
                     GAQVTDIAILVVAADDGVMPQTIEAINHAKAANVQIIVAINKIDKPSANPDRVKQELA
                     DHGLLAEDWGGDTICVPVSATQKTNLNTLLEMILLVAEMEELKANPNKKARGTIIEAQ
                     LDKGRGPVATVLVQNGTLKVGDPILAGSTYGRVRAMMDDKGRRVKSAGPSTPVEIIGL
                     SEVPSAGDAFYVVNSDKEARQIAHTLAERNREQNLRATPNKVSLDDLFNQIQSGDVKQ
                     LNIVIKADVQGSVEALRQSLERLSNDEVRIHTIHGGVGAITESDVMLASASNAIIIGF
                     NVRPEAGAKSFAEIEKVDIRLYRVIYNAIEDIEAAMKGLLEPEYKEKVIGHAQVRQTF
                     KASGLGTIGGAYVLDGKIIKNSQVRLVRDGIVVYEGQLSSLKRFKDDVKEVNSGYECG
                     FTLEKFNDIKEGDILEAFIMEEIPRA"
     gene            27176..27550
                     /gene="rbfA"
                     /locus_tag="PLG49_03925"
     CDS             27176..27550
                     /gene="rbfA"
                     /locus_tag="PLG49_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ16499.1"
                     /translation="MSKGTRIIRINEEIKKELSEIIRNEIKDPRIKDTLVSVVNVDTS
                     SDLKICKIYISVMGDKEQKENVIEGLKNASGFIRREIARRINLRNTPEFIFKLDDSIE
                     YSIHLSRIMDEISHNSGDDTHD"
     gene            27543..28499
                     /locus_tag="PLG49_03930"
     CDS             27543..28499
                     /locus_tag="PLG49_03930"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOQ16500.1"
                     /translation="MTSQEIYQLIHDSNYIAIAVHTNPDGDAIGACTALALVLKKIGK
                     KPIILMREIPDKYNFLAYHEFICQEYPKHLPLELFIALDCGDKERIGEYSNHFDIADT
                     TMNIDHHITNLEYADYNLVNSKASSTCEIIYKLIKDWEISLDQEISSALYTGIAFDTG
                     GFKHNNTSSYTHLIVADLLAYNINFTDIMDKLFHTRSISATKLLGYSLNKMEFYQNNQ
                     LCLCTLDKKELESFGAKDGDTEGIISFLKNTEGVLIACLLYEKTENETKVSFRSSGDI
                     DISYIAQKFGGGGHKKAAGCTINKNINEAKEEVINTILKELF"
     gene            28514..29407
                     /gene="truB"
                     /locus_tag="PLG49_03935"
     CDS             28514..29407
                     /gene="truB"
                     /locus_tag="PLG49_03935"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOQ16501.1"
                     /translation="MNGVINIYKEKGYTSHDAVADVRKILKQKRIGHTGTLDPEAEGV
                     LPVCIGKATKAVEYLMDFRKTYQATLEFGIITSTQDYTGEILSQKKVDFDEEKIKEAV
                     FSFLGEYEQIPPMYSAIKVNGKRLYQLAREGKTVERKARKVYIYHIDILSFLPPNQIK
                     IEVECSKGTYIRTLCEDIGNKLGFGAYMKDLIRTKVGIFEIENSIKINELRRQVELGN
                     IDKILIKTDDLFYQYNAVKIQYSGEKYLKNGNKIHYSYVEQLTNDIKIGDIVRVYSYK
                     DEFIGLYEIRSNYLKPLKLFL"
     gene            29419..30336
                     /locus_tag="PLG49_03940"
     CDS             29419..30336
                     /locus_tag="PLG49_03940"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOQ16502.1"
                     /translation="MKYLTNLDNPLDKPSVITIGNFDGLHLGHRKLISTVKELALAYH
                     YESIVFSFYPHPHEVLKKDDFPGMIYSREEKRERIEETGIDTYIEYPFTKEFASYSPE
                     EFVVNILIEKLHAKIIVIGSNNRFGKNQEGDVNFLKEREAKWDIKVVEISPVLDNSLI
                     VSSSRIREELMKGNIEKVNELLTLPYIISGTIVEGRKIGRQLGYPTANLLPKKDRLYP
                     PNGVYLTKTKYKNKIYNSITNIGYNPTVNGKNRVIETYIDDFCAMVYGEKITIEFYKW
                     LRAEKKFSSLDELVQQLDLDVKTCREYFN"
BASE COUNT        11201 a         4028 c         6014 g         9144 t
ORIGIN      
        1 ttcaacaaag gaggcactta cgtgaaaaat attttttcac cgggagttaa agttgaaata
       61 aaagaatatg gaacagatga taatgattct tattgctaca ccagccaaat tgaggaagtt
      121 ctaaaaaaag atgtgttagt acttggtaca cctattgttg gcggacaaat tgttcctctg
      181 agaataaaca ggatttatat cctctatatt tattcaagta atggtattta tagttgcaag
      241 gttcgtcctg tccgtcatta taaaaaaggg cttattgagt tggttcaagt agaaattatt
      301 tctgatttag caaaaataca aagacgagag tttttccggc atgagtgcgt tcagcccttt
      361 aaatttagaa tcaatgaaaa ttggtttgac ggtgttatta aagatattag tggtggcggg
      421 ataagattta ttagtaatga tgaattagaa gtaaaagacc atatttattg cagaattcct
      481 atagaaaatg acagtatcga aatcgaaggt attattttat ttaaagaaaa ggctaatgta
      541 gaactttaca aatatcaata cagagtaaaa tttatcaatg ttgttaaaac ggatcaggat
      601 aaaattgttc agtatatttt cgagcagcaa agaaaaaggg ttaggcagga aaaaggattg
      661 tgatgatagt ggataggaaa ataaaaatct tagttgttga tgattctgca ttcatgagaa
      721 gggttatttc tgatataatt aatagtgaca acaggtttac tgtagtcggt acggctaaaa
      781 atggggaaga aggtttagaa aaagttagac aattagatcc ggatgtcatc actttggatg
      841 ttgatatgcc agaaatggac ggactggaaa tgcttaagaa tctcatgaaa gagaatccaa
      901 aaccggttct cctaatcagt gcttatacaa aagaaggggc tgatacgact ataaaagcac
      961 tgggattagg ggcagctgat tttattacaa agccaagcaa tattttcaaa atgcaggaca
     1021 aagaaatcag agacaatatc attaaacgaa ttctcttgat ttctaacata tcaaattttc
     1081 cgtctgacaa taaaagacag ttaaaagaaa caggtcacaa taaacaaaaa gagcaagtac
     1141 atagtgtaaa gattaaaaag atagttgcca ttggaacatc tactggtggg ccaagagcat
     1201 tacaagaagt tttacctgag cttccggaac atttacctgc aacttatgtt attgttcagc
     1261 atatgccagc aggttttact aaatctctag ctgaacgatt aaacaactta tgtcagataa
     1321 aagtgaagga agcagaagat aaagatattt tacatccagg tgttgcttat atagccccgg
     1381 ggaactatca tattttagtt gaaaaaatgc cttattccaa tgatttgtgg ataagattgt
     1441 caagcagccc ttctgtttca ggtcatagac cttctgtcaa tgttatgctg aattctcttt
     1501 cggaattaaa tattgataac attgtagggg ttattatgac tggaatggga aatgacggtt
     1561 gtgagggcat gaaaaagctt aagctgaaca ataaggcata tgtcattgcc caggacgaaa
     1621 aaacttctgt catttatggt atgccaaaag ctgtaatcga cgaaggtatt gcagatgcag
     1681 ttgtaccgat taatcaaatc gccaaggaaa tcattagagt agtggaggtg ctttagcatg
     1741 gacatgagcc aatatttaga aatttttatt gaagaatcta aagagcattt gcaaagttta
     1801 aatgagtcat tacttcagtt agagcatgag ccaggtaata tgcccatact caacgaagta
     1861 ttccgtgttg ctcatacttt aaaaggtatg tctggaacca tgggctatac caaaatgcaa
     1921 aatctgacac ataatatgga aaatgttctt tcagaaatac gtaatggtaa tattgttgcg
     1981 gattcaaatt tattagatgt tttatttaaa tgtttagatg ctttggaaag ttatgtggat
     2041 gagataacaa actcaggaaa agaaggtaat gaagagtata aagatattat tgatgaatta
     2101 acgaatatct tatcaggaaa atcaaaaaaa gtggataata atgtagcagt ggcttccagc
     2161 ccagagataa aagcgcctgt gacagaagac aatataacaa ataatattac tgatgattcc
     2221 ggcgatgcaa tagatgttaa agaggcagct ttaacggatt ttgaaatcaa tgcagtaaga
     2281 gaagcctttg cccgcaatta caatgtatat caaattcata tattcttaaa tcatggctgt
     2341 gtacttaaat ctgcccgcgc ttttattgtt ttcagagcgt tagaaacaca tggtgatatt
     2401 attaaatcca tacctaaagt agaggatatc gaagacgaaa aatttgaatt tgattttaca
     2461 gttgtggtta tttctaagga tactaaagaa gattttgaaa aagatctcct ttcaattgct
     2521 gaagtagatc aggttattat taaaaatgta ttattaagtg ataaaattga actggcagca
     2581 gaagatgtga aagaagtaca taatacgcct gaatttgaca ggcaaagtaa cgttagcaga
     2641 agtatggctg atgatgagtc agatgataat gacagcagtg gcaaagcaaa acgtaaagca
     2701 ggaaaaactg tccgggtaga tattgacaga ttagacactt taatgaattt agtcagtgaa
     2761 ttgattatta ttaaaacaag gctcgagggt attgagcggg ataaaacgaa tcaaaactat
     2821 aatgaagcaa ttgaatatct tgaacgtata acaaccagcc ttcatgatgc tgtaatgaaa
     2881 gttagaatgg tgcctgtaga acgagtattt aaccgcttcc caagaatgat aagagattta
     2941 tccagaaaat tagacaaaga tattgaactt attatgtctg gtgaagatac agaattagat
     3001 agaacagtta ttgatgaaat tggagatcca ttgattcatt tattaagaaa tgccgcagac
     3061 catggtttag aaacgaagga agaaagacta catagtggta aacctgaaat tgggcatatt
     3121 tacctgcgag catatcaaga tggtaataat gttgtaatcg aagtagaaga cgatggaaga
     3181 ggtattaatg ttaataaaat taaacaaaaa gctattgaaa aaggtaccat tagttatgag
     3241 gcagctgcaa atatgtctga tcaagatatt atagacctct tatttaagcc aagctttagc
     3301 acctcagaaa aaatatctga tatatctgga agaggcgtcg gtttagatgt tgtaaaaact
     3361 aaaattgaag cacttggagg agatatagaa gttaaatctg agcttggtaa aggaagtaaa
     3421 tttattgtca gacttccact aaccttagca attatacaag ctttaatggt tagacttgga
     3481 aatgaaaaat atgcaattcc tcttaatact atacaaaata ttgaagatat taatgtctcc
     3541 gatattcaat tagttcaaca gcaagaagtt atccttttaa gaaaccaagt gattccaatt
     3601 atttatttag aagatatatt agatgtgcct aagaatgagc aatgcaatac aattacagtg
     3661 gttattgtaa aaaaaggtga aaaacaagta ggctttgtgg tagatggttt aatcggacaa
     3721 caagaaatag ttattaaatc tcttggtaaa tatttaagcg gaataaaaat gattgctggt
     3781 gcaacaattt taggcgacgg agaagtagcg cttattttag atattaatac attagtataa
     3841 ttgggggcga tgatatggaa ggtatttcca accaagaagt gaggcaattt atagttttca
     3901 aattaggaaa tgaagaatac gggattgata tattaaatgt acaaaatatt gaaagagtac
     3961 taagtattac ccgggttcct aaggctccct tttttataaa aggagttatt aaccttagag
     4021 gtgagattat tcccgtaatg agtttaagac ttaaatttaa tttagaagca gatgaatata
     4081 cggatgatac tcgtattatt atcgtgaaaa ttgatgataa tcaaatcggt atgattgtag
     4141 atgaagttaa agaagttctt caacttactg ctgattccat agaaagtgtt caaggtgttt
     4201 caggcatcaa tacggacatt aatttaagct ttattcaggg agttggaaaa gttaacaatc
     4261 atattgtaac cttgttaaat ataaaaagct taattgattc tattttagtt gatagtttag
     4321 aaaatgcata aagattgatc ggcggttagt tatatatagt tatatatata tttgatagat
     4381 ttccaaacta aagcattagg aaggaaatct atccctattt aaaggggtga aatagtgcaa
     4441 aacattgata tggtagatat tgataagtta aatcatttac atctggatat tttaaaagaa
     4501 ataggtaata taggtgcagg aaatgcgaca acggcacttt caaaactcat caataaaaaa
     4561 gtagatatga aagtgcctaa agttaatatt ttagaattta aaaatttagc agatatatta
     4621 ggaggacctg aaaatcctgt ggtcggcgtt ttattcgatg tctctggtga aattgaagga
     4681 atgatgatgt ttgtattgga acaacgtttt gctcatactt tggtaaatat tttaatggaa
     4741 agagacattc atcatatttc agagtttagt gaaatggata tttctgcttt aaatgaaatt
     4801 gggaatatat tagcaagttc atatttatct tctttgtctg cacttacccg tttaatgatt
     4861 gtaccatcta tacctcatct tgcctttgat atggctggag caatacttag tgttcctgcc
     4921 attgaatttg gaaaggttgg cgatagagta ttactcatag aaactgaatt tgcagagggt
     4981 atagaccgag ttgttgggta ttttatttta atacctgata ttaaatcatt taaaaaaatt
     5041 ttatcgtcat taggggttag tgtatgatga gttcgcaaga aataattaaa gtaggaatgg
     5101 cagatttaaa tacagcagta tatccggata ttctgaccac tttaggatta ggttcatgtg
     5161 ttgggatagc attatatgac cctgtggcaa gaattggggg gttagctcat ataatgctac
     5221 ccgatagcac ccaaattaaa aataattcta ataaggcaaa atttgccgac actgctacca
     5281 ttatcctggt tgaagaaatg attaaattgg gtgcagtaaa agaaagaatt gttgcgaaat
     5341 tagctggcgg tgcacaaatg tttgcattta ataatgcaaa tgaattaatg agaataggac
     5401 aaaggaatgt agctgcagcc catagtatac ttgaaaaatt aggtattaaa gttattgctt
     5461 ccgatacagg tgaaaattac ggtagaacca ttgaattata tactgaagat ggaagattag
     5521 ttataaaaac aatagggcac ggaataaaag agatttaggt gatagaatga acaaaaagtt
     5581 ggatattatt ctccctttga tttctgctat aataatttta agtatcggta tcataaaaaa
     5641 aacagcttat atcacaatta taaaagaatt aattatcatt attattattt tttactttat
     5701 agggaatatt tttaaaggac ttattaataa tatttattct gagtcagata aaaatgacca
     5761 ttctcaaatt acagctaaag ccgatgccaa tttaagctta aatgataata tcagtacaaa
     5821 ttctgaatcg gagggcaata ttcaagaaga agcaggcact caatagacaa gctttgtgac
     5881 aatcggtaag cgcgaaagat ttgtctctga aaacaactgc tttcaggagg aattatatgt
     5941 ctaaaaaaag tcttgaaatg ctatgggaac agtacgaagc cacaaaagat gtatccataa
     6001 aggatcaatt gattatggaa tacgccccat tagttaaata tgtagctgga agattgaaca
     6061 tttatttggg tcaaagtgta gagtatgaag atctggtaag ttatggtgtg ttcggtttga
     6121 ttgatgcgat tgaaaaattc gattttaaaa aaggagtaaa gtttgaaact tacgcttcat
     6181 taagaatcag aggatcaata atagataata ttagaaaatt agattgggtt ccaaggtctt
     6241 taagacaaaa aaataaagaa attgaaaaaa ccattatgga attagaaacg gaattgggtc
     6301 ggactgctac agacgaagaa ttggctcaga aaatgaatat ttccattgaa gaattacatg
     6361 agatttataa aaagacaaat cttatatctc ttatatcgtt agatgagtat atagaacaaa
     6421 ataatgatta taatataagc tctgaaaaaa gccctgctac tgaacaacca gaatcttata
     6481 ttgaaaagca agaattaaaa agaatactta aagaatccat tgaaaaacta ccggaaagag
     6541 aacaaaaaat tctttttctt tactattttg aagagttaac cttaaaagaa atcagttcta
     6601 ttatgggcgt ttccgagtca agaatttcac aacttcatac gaaagccatt acacgtttaa
     6661 agactaagtt gggaaaatat aaatctatct tatacgaatt ataaagagga ggatactcat
     6721 gtataatcct gacaagttca caaggagtgg atatcctaat tttgacggct tttttgattt
     6781 cgaattaaga gaagatggtc tttatcttat cctgcatgga cccatgggta aaggaaagcc
     6841 cgtagagctt gatgaaattt tgagccgcct tagacaaaaa aacataacaa attatgatta
     6901 tgctaaaatc aaagaggcag ttaaaaacat aaaagattcg gaaaaattag aaatatatat
     6961 ttctgatcaa caggagttag aagttatcga tgaagaagta gtcgtaaata tttcgaaaga
     7021 taaaatgttt gctgtcatta gttttattcc tcccgaaaac aatggaagac ttttatctta
     7081 tgaagaagtt atgcatgcaa ttgaatctgc aggcgttaat tttggtattg atgaaaatga
     7141 agtaaaaaga gccttagaag aaagaaagcc taattataaa tatgtgattg cgaaaggtct
     7201 taaacccgtt gaagggacac atggcaaatt agaatttcac tttaatgtta acaaggatct
     7261 taaaccaaaa gtcttagaag acggttctgt agattttcgt aatttagaca ttattaataa
     7321 tgtttcaaaa ggacaagttc tggttacgtt aattcctgcc cagccgggaa ctccgggaat
     7381 ggatgtatta ggaagagaaa tttttcctcc gaaagtaaaa aatccaaccc ttcctaaagg
     7441 aaaaaatacg gaaatatctg aagatggtct tagtttaatt gcaacgattg atggtcaagc
     7501 gatttatata aataataaag tgacggtcaa tcaaacttat gaagtgcctg ataatgtaga
     7561 caattcagta ggcaatatcg attttgtcgg taacgttatt gtaaaaggta atattttgac
     7621 tggcttttct ataaaagcag gtggtaatgt cgaagtttac ggtgtagttg aaggagcatg
     7681 cattgaagct caaggtgata tcattttgta tagaggaatt cagggaatga gcaggggaat
     7741 tcttaaatcc atgggaaatg ttgtagcaag atatattgaa aacagtacgg tcgaagctat
     7801 ggggaatgta accagtgaag ccattatgca tagcagggtt aaatgtggtg gcagtgttat
     7861 tgttgaaggt aaaaaagggc ttattgtagg gggcattatc agggcaggta aggaagtgaa
     7921 tgcaaaagtc attggttcac atatggcaac atctacagaa atagaagtag gtatagatcc
     7981 tacaattgtc gaaagatatc gctatttaaa agatgaacag gctaatatta aaaaagaaat
     8041 tattaaaacc aatcaggcta tcgacttatt aaataaaatg aaaggcacaa accgcttaac
     8101 cgatgacaaa agagcattat tgcaaaaatc gatccgtacg aaagtatttc tgaatgaccg
     8161 cttgaaaagt atcaagaacg aaatggctga tttggaaccc ttaatggaag aaagagatga
     8221 cggaaaggtt cgtgctttta atgttattca tccaggtgtt aaagttacca ttggaacagc
     8281 tacaatgtat gtaagagaag aaattaaata ttgtacgtta tataaagata atgctgatat
     8341 aaggacaact agttattctt agggggtgaa aatgtgtcca tacgtcctgt tgatatgcaa
     8401 gtgattattc caaaaacgac agaaatatca aaacaaaatc aggaacagac atataaaggt
     8461 gaaatacagc aacaggaatt tgcacagcag atggaaaaaa agattatgca acatcagcag
     8521 caagtggtga ataccaataa atctgaatat gcagtcaatg atgaaggacg taataaaaat
     8581 cagaatcagg gcaatcaggc caagaagaaa aaaaataaaa atgaaaaaga taaaaaatcc
     8641 aatatttctg atagtaacag catcatcgat ataagaattt aattgaggtg tataccatgt
     8701 tgcaaagtga aattttaaat attatgattt tttttgtaat cagcatagga atgattatac
     8761 taattattgg acttgttttt ataaaagaaa aaaatcaggg ggagcattcc agcaataaaa
     8821 atgagaattt tgaaaaggaa ctggaatcac aaattaatga agccgataaa atgcttcagg
     8881 aactcaatca tttttcttcc tatattaaag aagaattaga taataaatat aaggaattat
     8941 tgtttatata tcaattaatt gatgaaaaag gtaaagagtt atcaaatgat aagattcaac
     9001 tggttagtga gaaaaatgat aaaaacagta gcgacaaaga taataatgct catgacttgg
     9061 aagctttaca aattttaaat aataagaatt acgatcagat tatcaaattg tataaagaag
     9121 gaaatgatat ttcaagcatc gccaaaaagc tcaatattgg aaaaggagaa gttcagttaa
     9181 tactcggact agcgaaaatg aggtgaagta atttgggatt atctacaaga agttttatga
     9241 taggattggg cttagggatt gttattacag gaataacttt ttgttttatt ccttataagt
     9301 cgatatctga tgacgaaatc attcaaaaat caaaagaatt aggcatggta aatcctctcg
     9361 atttgtcacc agaaaaattt gttttatcac aggaagaaat cataagaaga gctaaaaaac
     9421 ttggtatgga ttttgttaaa aaggacgata atttaaatga aaaagatgaa tctcatgaat
     9481 attatgagct tgatgtggtt accatattta taccacaagg tcttgattca aaagaaatca
     9541 gtagtttatt gtacgaaaat catttaattg atgatcgtga tgcctttgat tcatatcttc
     9601 ggaataataa tttagacaga attattgctt acggatattt tgacattcct acaaaaagcg
     9661 attataaaac cattgctgat ataataacca gaagaagcca tgattagtga aaaataatcg
     9721 tggcttttta atgaaattta tcattttatt aaagttaatt attgattata ggatgattat
     9781 gtgctatact tccatgtgga aataaaacac acgcatcgga ttttctaata tggtgccaag
     9841 aggtctttta gaaaagatga cgattgcgga ggaaaaaacc aaaataggag gagaaaaaaa
     9901 tgagtgtaat ttcaatgaaa caattattag aagcaggtgt tcattttgga catcaaacaa
     9961 gaagatggaa tcctaaaatg gcggaataca tcttcacaga aagaaatggt atttatatta
    10021 ttgatcttca aaagactgtt aagaaaattg aagaagcata tcaggttatt cgtgatgttg
    10081 tagaagaagg tggcaatgtt ctttttgtag gtactaaaaa acaagctcag gattcaatca
    10141 aagaagaagc tgagcgatgt ggaatgttct atgtaaatca aagatggtta ggtggtatgc
    10201 taaccaattt caggactatt cgctcaagaa ttgagagatt aaaagaatta gagcgaatgg
    10261 aagaggacgg aacttttgat gttcttccta aaaaagaagt cataaagctt cgtcatgaaa
    10321 aagaaaaact tgaaaagaac cttggcggta ttaaagaaat gaattcttta ccagatttaa
    10381 tttttgtcgt tgatccgaga aaagaaaaaa ttgctattca agaagcacat attttaggaa
    10441 ttcctgtggt agcgattgta gatactaatt gtgatcctga agaggtagac tatgtcattc
    10501 ctggtaatga cgatgctatc cgtgctgtta aattaattgt tgctacaatg gccaatgcag
    10561 ttcttgaggc taaacaaggt gttcaaacct ctgttgcaga tgaaaaagta gaagcagaag
    10621 aagtagtagc agaaaacgaa gttgaagaag cagtagaaca agtagaataa tatttttaat
    10681 agagacgata aatattattt gttaatgcaa tatgcatctt gctaaaactg cctttataaa
    10741 agaattgcgg caaatgccgc atttcttttt aaaatattga tcctggtgat aataagtttc
    10801 atcttttcta taataaaatt aaatatttgg aggttgaaaa atggctatta cagcaaatat
    10861 gattaaagaa ttaagagaaa tgaccggtgc aggaatgatg gactgtaaaa aggcattaac
    10921 tgaagctgac ggtaatatgg aaaaggctgt agaaatatta agagaaaaag gattagcaaa
    10981 agctgctaaa aaagctggtc gtattgctgc agaaggttta gtagctactt atgtatctga
    11041 agatggtaag actgcggcat tagttgaagt taatagtgaa actgattttg ttgcaaaaaa
    11101 tgaattattt aaaacttttg ttgacgaaac tgctaagcaa gcagtgctaa caaaaactga
    11161 caatattgaa gattttatga aagaagcttg gcatttagat aattcgaaaa cagttcagga
    11221 tgttttaact gaaaaaatct ctgttattgg agaaaattta aatatcagac gttttgtaaa
    11281 atatgaaata gaatctggta tcattgcaac ttatattcat ggcggtggaa gaatcggtgt
    11341 aatggttcat ttagattgca attcttcttc tgataaattg gctgaagcag gaaaaaatat
    11401 tgctatgcaa atagcagctg tttatcccaa atacattacg attgatgaag ttcctgctga
    11461 ttatgttgaa aaagaaagag aaattttaaa gcaacaagct ttaaatgaag gaaaaccagc
    11521 taatattgtt gaaaaaatga ttgaaggtcg tttgaaaaaa tctttaaagg aaatttgttt
    11581 aatggatcag gcatatatca gagatcctga aatgactgta agccaatatt tagacagcgt
    11641 ttccaaagaa attggtgata aagtaactat taaagctttt gttcgttatg aaaccggaga
    11701 aggtatcgaa aagaaagaag aaaattttgc agaagaagtt gctaagcaaa tgaatcaata
    11761 aatagtgata ttttgattta acaaaggaga cacatagtgt ttcctttttt ttctgaataa
    11821 tataaattca aattatagtc atttaaattt tgatataata tctatgaata aatattgtat
    11881 aattatctgg aaaaatataa aaaaatatga tacattaata acggaggaat tattcaatga
    11941 gtagctataa aagagtgtta attaaattaa gtggtgaagc attatctggt acaaaaggat
    12001 atggttttga ttatgatatt gtgaattctg ttgtaaagca gttaaaaact gtagttgagc
    12061 aaggtactga actcgcgatt gtaattggtg gaggaaactt ttggagaggt cgcaccgata
    12121 ataaaatgga tagaacgaag tcggatcaaa ttggcatgtt ggcaacggtt atgaattcat
    12181 tatatgtggc agaagtatgc cgggcaaatg gacttaatgc ggttgtccaa acccctttcc
    12241 aaattggaac aataactgaa ttgttttcta aggattcagc tcttgaacat atgaaaaata
    12301 aaaccgttgt attttttgct ggtggaacag gtcatcctta tttttcaaca gatactgggg
    12361 ctgcattaag gggatgtgag atcgaagctg atgttttact atttgctaag aatatagatg
    12421 gtgtatatga ttctgatcca aagtttaatc ctaatgcaaa aaaattcaca gaattaactg
    12481 gaaaagacat aatagaaaaa caactaaaag ttatcgatac tgcagctgcc agtctttgta
    12541 tggatcagca cttagatatt ttaatatttc ctttaaattt tgaaaatagt ataatcgatg
    12601 tgatttcagg taagaatata ggaacaataa taaaaaatta aaagatttaa gggggtaatt
    12661 agatatggat aagcagtatg aacaattcga aacgaaaatg caaaagtcaa ttaatgcatt
    12721 ggaaaaagaa ttaaacatga tcagagcagg cagagcaaat cctcatattt tggaccgtat
    12781 ttttattaat tattacggag tacagactcc cgtcaatcaa gtagccaata ttactgtacc
    12841 agaagcaaga atgatacaga ttcaaccttg ggatgcatcg gtcataaaag atattgaaaa
    12901 agcaatttta gcttccgata ttggaataac tcctagtaat gatggtaaag tgatacgttt
    12961 agtattccca gaattaaccg aagaaagaag aaaagaatta actaaagaag taaagaaaaa
    13021 aggtgaagat gcaaaggttg ctattagaaa catacgtaga gaagctttag atcatttcag
    13081 aaaagcagaa aaagaaggag aaatttcaga ggatcaattg aaagaaattg aagaagatat
    13141 tcaaaaaatg actgataaat atatagaaaa aattgatgta catattgaaa ataaaaataa
    13201 agacattctt tctatatagc atagtatata aagccccttt tataaggggt gttttcttaa
    13261 ttttacgcat tgtgttatct tattgctgtt attacatctt gcaccagatt tgaggaggtc
    13321 tgtatggata accataatta taaaaataag ttaaaaatgg attctctccc aaagcacatt
    13381 gcaattatta tggacggaaa cggccgatgg gctaaacaaa aacaacaatc ccggtctttt
    13441 ggtcacaagg caggttccaa agctttagaa agaattgtta aagcggcgga ttcattaggt
    13501 atcgctcatt tgacggttta cgctttttca acggaaaact ggaagcgccc aaaagaagaa
    13561 gtcaatgctt tgatgaaatt attaagtcaa tacctaaatc aatatcttaa ggatacgaaa
    13621 gataataatg taaaaattga tgtcattggt gacataacaa aatttgacga agaaatacaa
    13681 agaaaaattg aaaaaattca agaattgact ttgaaaaaaa atggactcaa tttacatatc
    13741 gctttaaact atggaagtag agatgagatg acccgtgctg ttaaaaaaat tgttacagat
    13801 gtaatggaat ctaaaataga tttgaattct attgatgact ctttaatttc ttcttattta
    13861 gatactaaag aaattcctga tccggatctt ttaatcagaa caagtggtga aaaaagaatt
    13921 agtaatttct tgttatggca aattgcctat accgaatttt attttacgga taaattatgg
    13981 ccggattttg acgaaaatga tttatataag gcaatttttg aatatcaaaa tagagaacga
    14041 cgctttggaa ctatttaata tcggaggaga ttttattggg tatacgtata ttaacggcac
    14101 tcttagggat acctttactt atatttgtta ttttatcggg tggtttaata ttaaaaattg
    14161 cattatgcgt ttttgtttta attggcatgt atgaattcta taaagctatg gataataaat
    14221 ttaatccaat aaagccgtta ggttatattt ttgccttatt ttatatctta tttatttcgt
    14281 caggacagca gtttgctttt ccttttatga ttttattttt aataatattg ttattagcta
    14341 ccgttttttt gcataaaaaa tataaggtcg ttgatgtggc aattaccttt tttggatttt
    14401 tttatgtatg tgttttgttt tctcacattg ttcaaataaa tcagctttta catggagaat
    14461 tatttatttg gttaatcttt attagtgcct ggggtagtga tactgcagct tattttactg
    14521 gtatgttttt aggcagacat aaactttgtc ccagtctaag tcctaaaaag actattgaag
    14581 gagccatagg aggttttatt ggaagtgttc ttttatcact tgtatacggg ataatcattc
    14641 agtctggttt aaacgtttca atgaaccatt tactccttat ctgtattgtt gttggaggac
    14701 ttggaagtat agcttcgcaa ttaggagatt tagcggcttc ctccattaaa aggtatgttg
    14761 aaataaagga ttatggttat ttatttccag gacatggcgg cgtattagat cgttttgaca
    14821 gtattttatt tacagctcca ttagtctatt acatactttt attatttaat tttattatag
    14881 gctaagttat tgtcaaaatt tccattctaa gattctttgg actgcttgga ctacaaaatt
    14941 aagaggtgag ttttttgaaa gcaatttcta tattgggatc cactggctca ataggtactc
    15001 aaactttaga tgttgtcaga aatttaggaa atatcgaagt aaaagcacta tcaacgaata
    15061 aaaatataga cttattagaa aagcaaatag aagaatttca tcctaaaaag gtagcagtaa
    15121 tggatgatga aaaagcatat gaattaaaaa agagaattgg aagcaaaata gaggtgttaa
    15181 aaggattaga aggcctttgt gaaattgctg aaatggatga aacagatact gtggtaactt
    15241 ctgttgtcgg tgttatagga ttgattccta cttttaaagc gatccaaaag aaaaaaaata
    15301 ttgctttagc taataaagaa accttggtaa ctgcaggtaa aattattatg gaagaagcca
    15361 aaaaaaataa tgtgtccatc tatccggttg atagcgaaca ttcagctatt tttcaatgtt
    15421 tgcaaggtaa tgcccataac aagataagta ggattatact tactgcttcg ggaggtcctt
    15481 ttcgtggtaa aagtttaaaa gaacttgaaa tggttacagt tgaagaggct ctgaagcatc
    15541 ctaattggga aatgggagca aaaataacta ttgattctgc aaccatgatg aataaaggct
    15601 tagaagttat tgaagccaaa tggttatttg atgtagaatt aaatcaaata gaagttttag
    15661 ttcatcctca aagcattata cattctatgg tagaatttga agatggctcc attatggctc
    15721 aaatgggtga gccggacatg agactgccta ttcaatatgc cttaacttat cccaaacgga
    15781 taacaaataa ttggccaaga attaactttc gtcaaagaaa tatgctttct tttgaagaag
    15841 ttgatttgtc cgtttttaag tgtttacaat tggcctacga tgcccttaaa atagggggca
    15901 caatgccagc agttttaaat gctgccaatg aagttgccgt agataaattt cttaaaaaag
    15961 aaattaattt tttagatata gcaaaaatta ttgaagctac aatggaaaaa cacgagttga
    16021 ttaaaaatcc ttctttatcc gatatattag atgtagacca atgggcaaga aaaatatgtt
    16081 aattgaggtg aaaaaatgga tgtattgacc atttttatat caattttagt tttcggatta
    16141 atcatcctgg tgcatgaatt tggacatttt attgtagcaa aaaagagtgg gataatggta
    16201 gaagaatttg ccatgggtat gggacctaaa ctaattagta aaaagattgg tgaaactgtt
    16261 tattctattc gtgcatttcc tattggcggt ttttgtaata tgttgggagc agatacagct
    16321 accgatgaca gtcgttcttt tacaaacaag ccagtcagga caaggattgc tgttattgtt
    16381 gctggtcctg tcatgaatct tctgttagcc tatatcatta ttgtttggtc tgtagcatct
    16441 ggtggctatt taagtacaac cgttaacaga gttcttccca attcagcggc agaagaaata
    16501 ggtctgcaag caggagataa gattatttct ctggatggac aaagaataca tcttaaacaa
    16561 gagattgata tgattgttca gcaaaccaag ggtagtccac ttcaaatcgt tattgaaaga
    16621 gacggaagcc ggataacaaa acttgtaaca ccgagacaag aaggtgaccg ctggttaatt
    16681 ggttttgaac ctaaaattgg agagaataat gtatttaatg ttctcagtca ggctttttgg
    16741 caagtcatct ttataattaa acaagttatt attggactga tacaactggt tactttaaaa
    16801 gctaaaggac aagttgcagg acctttaggt gttatggata ttattggtga agcttatcag
    16861 gaaggtatga aagccagctt ttacgtagcc atccaatatc ttttaagtat taccgcttta
    16921 attagcgcca atctgggtgt aatgaatttg ctgcctttcc ctgctttgga tggaggcaga
    16981 ttaatattct tatttttaga aggattaaga ggtaagcctg ttgacgttga aaaagaagga
    17041 ttaattcatt ttattggttt tgctgtactg atgacactga tggttgttgt tttatttaat
    17101 gatatatcgc ggatttttat ccattaataa tggaggcaat atttcatgat aaatattaag
    17161 cgaaaaaaaa caagaactat tttcattgga aatcttccaa ttggctcgga acatcccata
    17221 gccattcagt ccatgtgtaa cacagacacg agaaatgtag aagaaactgt aaaacaaatt
    17281 caccttttag aagaagcagg ttgtgaaatc ataagagtgg ctgttccaga tatggaggca
    17341 gctaatgcca ttgctgcaat taaaaatcaa atccatattc ctttagtggc ggacatccat
    17401 tttaattatc gcctggcttt aacggccata gaaaaagggg tagataaatt aagaattaat
    17461 cctggaaata ttggttccag ggaacgtata aaggaagttg taaaagcagc aaaagaacga
    17521 aatataccta ttcgtatagg agttaatgcc ggctctttag aaaaagattt attagataaa
    17581 tatggcgttc agcctcaggc actggttgaa agtgctctaa gacatatccg tattttagaa
    17641 gaattggatt attataatat cgttgtctcg attaaagcat caagtatacc tttgtcttta
    17701 atggcttatg aagctttatc tgaacaaatc gactatccgc ttcatgtcgg catcactgaa
    17761 gctggaacta taaaaagcgg aagaatgaaa tcggcagtag gcattggggc aatcttatca
    17821 agaggtatag gggataccat aagggtatcc ttgactggag atcctgtaga agaagtgctc
    17881 tgtgccaaag aaatattaca agctcttgaa ttaagaaaat ttggtgtgga actgatttct
    17941 tgtcctactt gtggaagaac tgaaatagac ttgatttctt tagccaatga agtagaaaag
    18001 agatgcgcta atatcaataa aaacataaaa attgctgtca tgggctgtgt ggtaaatggt
    18061 cctggagaag caagggaagc cgatattggc atagctggtg gaaaaggtgt tggtctcatt
    18121 tttaaaaaag gtgaaattat taaaaaagtt ccggaaaatc aattgttaga agcattattt
    18181 gaagaaatcg acaaacttta attatagtaa ttgtccatag gacagatgga ggttaaaatg
    18241 ctacaaaatg aaaaaaacca taacttttta gatgtttttc ctcagttgca tgttgacgct
    18301 ccaatcaaag aaatgctaaa agatgctaag atcaaaaaaa taaatgttta taaagaagat
    18361 aaagctatag aaatcttact ggatgtaaaa aatattattt acagggaaat tcttgaacaa
    18421 ttaagcaggc aaattcagaa acagtttaaa ggaatcaata aagtatattt aaagtcaaaa
    18481 tatatttcag atgatgattt attaaataat aaggattcca aaagcaaagt gattcatttg
    18541 tattgggata gtattcttca ttcgataaat gataaacatc ccattggtac tggtatttta
    18601 aaagattgcc attggaaact gaaggataat tatctggtat taaaagtaag taacaatcag
    18661 tcatcgtatt taaggaagaa tgaattggat aaaaaaatta aatggatgtt aaacgaagaa
    18721 ttaggactga acatggaagt tgtatttgaa gaagaaatga ttacaaaaga taaaaagaaa
    18781 atttatgaag ataatttatc aaatatagag catgatattg ttgagaatct gctggcaaca
    18841 agtactacac aggtcattaa tactgaagaa gtaaaagatc atatgccgga taaggaaacc
    18901 ccggatcctg ttatcctggg gaaagatata aacagtgaag ccattcgaat aaaagacatt
    18961 actgaagaat ctgacgaagt tgttattcag ggagatattt tgtctataga atacagagag
    19021 attaatcatt caaaatatat cgttaccttt gacattacag attatgaaga ttccataacg
    19081 gtgaagtttt ttgtaaagaa aaatatcttt gaaaacgcca tcaaaaacag gttaaaagaa
    19141 aaatgtcatg tctgggttaa aggtttgatt caatatgata atttttctaa agaaattgtc
    19201 ttaatggcaa aagatatcgt tgaaaagact tcaaaattaa tcagaatcga tcaagcccag
    19261 gaaaagagaa tcgaattgca tgcccataca caaatgagtt ccatggacgg gattgtttcc
    19321 gtaaccgctt taattgaaag agctgcaaaa tgggggcatc cagctattgc tattactgac
    19381 catggcgttg tacaagcata tcccgatgca gcgtccgcat caaaaaaata tggcattaaa
    19441 gtgatttatg gaattgaagc ttatttagtt gatgatttaa atgcagtgat tcaaaatcca
    19501 caaaatcaga ctttagagga tgaatatact gtttttgata tagaaaccac agggctgtct
    19561 ccaacaaaag ataaaattac agaaattggt gctattaaaa ttaaaaatgg aaaaattatt
    19621 gattatttta gctgttttgt taaccctgaa atgcccatcc ctgctaatat tcaagagtta
    19681 acgggtataa cagacgagat ggtaaaagat gcagcgacca tagaacatgt gctgccagac
    19741 tttctgaaat ttatagggga cagtgttctt gtagcccaca atgccaattt tgatgtgagc
    19801 tttatccgta ctgcggctga aaatatgtct ttatctgttc aaaatactgt tctagatacc
    19861 ttggaattat ccagattatt atttccttca ttaagcagac ataagctgga tattgttgcg
    19921 aaacatttag gggtttcttt agaaaaccat cacagagctg tagatgatgc aaaagcaact
    19981 gctgaaatat ttattaagtg catagatatc ctgaaagaaa aaaatataca aaatttaaat
    20041 gaaattaatg ctttagcagg tgcagaaatt gatgtaaaaa agcttaaaac ttatcatgca
    20101 gtgatattgg ttaaaaatca aacaggcctg attaatttat ataaattagt gtccttttct
    20161 catctggagt attttaataa aagacccagg atacccaaaa gtctttattt gaaatataaa
    20221 gaaggattaa tgatcggaac tgcctgtgag gcaggagaac tttacagagc tatccttaat
    20281 aaaaagtcag atgaagaaat aaaaagaatt gcctcgtttt atgattatct agaaattcaa
    20341 cctttggata ataatcaatt tttaattgac aatggtacag tcgaatccgt tgatgaactt
    20401 aaaaaaatta atatgaatat tgtcgaatta ggaacattgc tcaataaacc tgttgtggcc
    20461 acctgtgatg tgcatttttt agatcctcag gatgaagtct ttagaagaat tattatggca
    20521 ggacagggtt tttccgatgc cgataatcag gcgcccttat atttacggac aacagaagag
    20581 atgcttgaag aattttctta tttaggagag aaaaaagcca gagaagttgt tattgataat
    20641 ccgaataaaa taaaccaatt cattgaaaat attaaaccta ttccggatga aacttttcct
    20701 cctaaaattg aaggagcgga agaggaatta acaagaatta caatgaaaaa agcgaagtcc
    20761 atttatggtg atcctcttcc tccagcagtg caaaacagat tggacaggga attaaatgcc
    20821 ataataaaaa atggttttgc tgtaatgtat attattgcgc aaaaattggt ttggaagtct
    20881 ttggaagatg ggtatttggt tggttcccgt ggttctgttg gttcttcttt tgtggctacc
    20941 atggcatcca ttacagaagt aaatcccttg cctcctcact atatttgtcc taattgtaag
    21001 tattccgatt ttgattcgga aatggttaaa tcttttgccg gaggctctgg ctgtgatatg
    21061 ccggataaga tttgtcctaa gtgtggagaa atgatgaata aagatggtca cgacattcct
    21121 tttgaaactt ttttaggttt tgatggggat aaagagcctg atattgactt aaacttttct
    21181 ggagaatacc agcccagagc tcatagttat acagaagaat tatttggaaa agactatgtt
    21241 tacagagctg gtaccattgg aacattagca gaaaaaactg cttatggctt tgttaaaaaa
    21301 tacatggaat ccaaaaacat cgttttaaga aaggcggaag tcaaccgatt agtaaaagga
    21361 tgtacaggaa ttaaaagaac aacagggcag catccggggg gcttaatgat tgttcccaaa
    21421 gataaagaaa tatatgaatt ttgtcctgtt caaagacctg ccaatgatgt gaattctcct
    21481 gttattacaa cacactttga ttatcattcc attagtggaa gattgttaaa attggatatt
    21541 ctaggtcacg atgaccctac agttatcaga atgcttgaag atttaacggg agttaatcca
    21601 agaaaaattc cattagatga tgaaaatacg atgtctttat ttagctctac gaaagcatta
    21661 aatgtgagtc ctgaacaaat taactgtgaa gttggaactt taggtctgcc tgaatttggg
    21721 acaaaatttg taagacaaat gctaatagat acaaagccaa aaacattttc agaactcata
    21781 cggatttccg gactttcgca tgggaccgat gtttggctta ataacgccca ggatttaatc
    21841 cgggatggaa cagcaagcct ttctgaagtg atttgtactc gggatgatat tatggtttat
    21901 ttaatcaaca aaggagtaga aaagaaacac gctttcaaaa ttatggaaaa ggtcagaaaa
    21961 ggaaaaggtc ttgagcctga tgatgaagaa gctatgaaag ctgctggggt tcctcaatgg
    22021 tatatcgatt cttgtaaaaa aattaaatat atgttcccta aagcccatgc tgcagcctac
    22081 gttatgatgg ctttcagaat tgcgtatttt aaagtttatt atccggaagc tttctatgca
    22141 acttatttta ctgtcagggc agaggatttt gattattgtc tcatgtgtaa aggggaaaac
    22201 aaggttcgta aagccatcaa agaatatgaa gaaaaaggta ataatctgac aacaaaggaa
    22261 aaagggaccc ttactgtttt agaaattgtt ttagaaatgt acgcaagagg atttaaattt
    22321 ataccaattg atttatataa gtctgacgct gtaaccttta aattaacacc agagggcatt
    22381 ctgcctcctc ttaatgccct tcaggggctg ggaaacactg ctgcttttaa tattgttaaa
    22441 gctagagaag aaggagaatt tctttctgtt gaagatttcg ttgaaagggc aaaaattagc
    22501 aggccagttg ttgaaattat gagagaaaat gaaattttga ccgggatacc cgaaagcagt
    22561 cagttatcct tgttttaata cttgcattta tgaattgatg atgatataat aaaaacagtt
    22621 ggtatgtata ttctttattt aagagtgggt cgaagcccac tctttctatt gtatattgag
    22681 tttttaatat cccattgtcc cttactttat gtagaaagat gatgaatagg atattctttt
    22741 ttatttaaaa ctatataaag aattataagt acggattaat tcattccatg cttatccaat
    22801 gatatcattg aaagggtgat tatgtgtcaa aacaaaacat agagcaaacg gttgaaacat
    22861 atcttaaacc tattttagat gagcacaatt ttgaactggt cgatgttgaa tacgttaaag
    22921 aaggtacaaa ctggtacctt agagttttta tagataaaga aggaggcata accattgacg
    22981 attgtgaatt agtttccaaa gctttagaaa aaattctgga caaagaagat cccattaaaa
    23041 actcatatat tttagaagtc agctctcccg gtttagacag acctttaaaa aaagattctg
    23101 attttgaaaa atataaggga agaattgtcg atattaaact ctataaacca tttaataaaa
    23161 agaaagaata ttctggtgag ttattagaat taagagataa tatcgtttac atcatagatg
    23221 aagaaggaaa ccagctttct tttaacagga atgatattgc cattgtccga ttggcagtta
    23281 tattttaatc attttgagga ggtaaagggt atatgaactc cgaatttatc ggtgcaatcg
    23341 aacaaattgc taaagaaaaa ggaattgaca aagaattgct ttttgaagct atagaaactt
    23401 cattggtgtc tgcttgtaaa aagaattttg gtacttcaca aaacattaaa gtttctattg
    23461 atcgtgaaaa tggggatgtc aatgtttatg cacaaaaaac ggttgtagaa aatgttgaaa
    23521 acagtgcttt agaaatttct ctggaagaag ccagagaaat tgatattaat tatgaactgg
    23581 gagatattgt tgacatagaa gttactccta agaattttgg ccgtattgca gctcaaacag
    23641 ctaaacaggt agttattcag agaattcggg aagctgaacg ggaaatcatt tataatcatt
    23701 atattacaaa agaaagagat atcatgacgg ggattatcca aagaaaagaa agaaaaaata
    23761 ttatgattaa cttaggtaaa gctgaggcta tccttcaacc caatgagcag atccctacag
    23821 aagagtatga actgaacagc agaataaaag tttatatcct tgaagttaaa cagaccacta
    23881 aaggatctca aatatttgta tccagaaccc atccggaatt agttaaaaga ttatttgaac
    23941 aggaagtccc tgaagtatac gaaggaatcg tagaaattaa aagtatcgcc agggaagctg
    24001 gatccagaac taaaatggca gttcattcca atgatccgga agttgaccct gttggtgcct
    24061 gcgtaggtca aaacggtcaa agagttaatg ttatcgttaa cgagttaaga ggagaaaaaa
    24121 ttgatattat acaatggagt gatgatccaa aagaattcat tgcagctgct ttaagccctt
    24181 cgaaagttat taaagtcatt acagatgaag aaaataaaag tgccagtgta attgttccgg
    24241 attatcaatt gtctttagcg ataggaaaag aaggacaaaa tgcaaggctt gctgcaaaac
    24301 tgactggttg gaaaattgat attaaaagtg aatcacaggc aaaagatgca ggaatggtgc
    24361 ttgaagaaag ctcagatcaa gacgatgaag caaattcagg catagaggag tgatacaatg
    24421 aaaaaaagaa aaattcctct cagaaagtgc actggttgtc aggaaatgaa aaataagaaa
    24481 gagttaatta ggattgtaag aaatcaagaa ggagaaatat ctttagattt tacaggaaaa
    24541 aaaccaggac ggggtgcata catttgtccc aatgaagaat gtcttgaaaa agcacaaaaa
    24601 tctaaaggct tagaacgctc ttttaaaaca gcaatacctg atgaaatata cgaagaatta
    24661 aaaagtgagc tgaaaaaagc aaaatgagta attcaattta ttccatgtta ggtttatgta
    24721 tgaaatccgg aaatctttta tctggtgaat ttcaatgtga aaaagctgtt aaagccagaa
    24781 aagcaaaatt agtaattatt gcaaaagatg catcttataa cacaaaaaag ctatttaaag
    24841 ataaatgcag ttatagaaaa attccttttt atgaatttgg tacaaaggaa gaattaggaa
    24901 gggctatagg aaaaagtcca cgttcttccc tggcagttat ggatgagaat ttttctgcac
    24961 agcttattaa aatgtttgat tcttgtaagc tgtaatttat ggaggtgagc tatttgtcaa
    25021 aagtgagagt atatgaaata gcaaagaaat taaacataag tagcaaggaa ctgatagaag
    25081 tgttagaaga ctttggaata aaagtacata gtcatatgag ttccttagaa gatgaagaag
    25141 cacaattaat tatggaatat tattcaacag ataaagataa tgatataaga gacaataaga
    25201 aatcttctga ttctaaagat aagaagaaaa agacaaaatt aaaggataca aaaacagaat
    25261 ttggagaaga gctgcatcaa aggacagata aaatgaatga acaaaaatct actaaaaaag
    25321 ataaggcatt taaagaaaag gagaaagaag ttaaatcaga caaaaaagat aaggaagaag
    25381 atactataaa aattattaaa atttctgata aaattactgt gcaatcgctt gctcaaaaac
    25441 tggactgccc agcaacagaa ctcattaaaa aattaatgat gaaagggatc atggcatcta
    25501 tcaatcagga aattgacttt gataccgctt ccatgatagc agaagaatac aatactttag
    25561 tagaagaaga aaaagaagaa gatttcttgg aatctctttt tgccgaagaa gccgattcag
    25621 aagaaaacct tgttgaaaga ccacctgttg ttgttgtaat gggtcacgtt gaccatggaa
    25681 agacatcttt actggatgct ttaagaaaaa caaatgtgac agcgacagaa atgggcggca
    25741 ttactcaaca tattggggct tctgtgattg aagtcaatgg aaagaaaatt acttttcttg
    25801 atacacctgg acacgaagct tttactgcca tgagaatgcg aggggctcaa gtaacagata
    25861 tagcgatatt ggttgtagct gccgatgatg gtgtaatgcc tcagaccatt gaagcaatta
    25921 accatgctaa agcagccaat gtacaaatta ttgttgccat caataaaatt gataaaccca
    25981 gtgctaatcc cgacagagta aagcaagaat tagctgatca tggtcttctt gctgaagatt
    26041 ggggtggaga taccatatgt gttcctgttt ctgcaacaca aaaaaccaat ttaaatactt
    26101 tgttagaaat gattttatta gttgcagaga tggaagaatt aaaagcaaat ccaaataaaa
    26161 aagccagagg aacaattatc gaagcacaat tagacaaagg ccgtggacca gttgcaacgg
    26221 tactggttca aaatggtact ctcaaagtcg gagatccaat attagctggt tcaacctatg
    26281 gcagagtaag agcgatgatg gacgataagg gccgccgtgt taaaagtgcc ggaccttcta
    26341 ctcctgtaga aatcattggt ctgtcagaag taccttcagc aggagatgct ttttatgtgg
    26401 ttaatagcga caaagaagca agacaaattg cacatactct ggctgaaaga aacagggaac
    26461 aaaatttaag agctaccccc aataaagttt ctctggatga tttatttaat cagatacaat
    26521 ctggtgatgt aaaacaatta aatattgtta ttaaagccga tgttcagggt tctgtggaag
    26581 ctttaaggca aagtttagaa cgtttatcca atgatgaagt aagaattcat accattcacg
    26641 gcggggttgg agctattact gaatccgatg tcatgttagc atctgcttca aatgcaatta
    26701 ttattggatt taatgtccgc ccggaagctg gtgctaaatc ttttgctgaa atcgaaaaag
    26761 tagatatccg tttgtatcgc gttatttata atgccattga agatattgaa gctgctatga
    26821 aaggattgct ggaacctgaa tacaaagaaa aagttattgg tcatgcccag gtacgtcaaa
    26881 cctttaaagc ttccggactt ggtaccatcg gaggtgctta cgttttagac ggtaaaatca
    26941 tcaaaaattc tcaagtcaga ttggttcgtg atggtatcgt cgtttacgaa ggacaactta
    27001 gctctttaaa gcgttttaaa gacgatgtta aagaagtcaa tagcggttat gaatgtggtt
    27061 ttacgctcga aaaattcaac gatatcaaag aaggcgatat ccttgaagcc tttataatgg
    27121 aagaaattcc gagagcataa ttaaaaattt accataaatc agaatggtgg ttgaaatgtc
    27181 aaaaggaaca agaataataa gaataaatga agaaattaaa aaagagctta gtgaaataat
    27241 tagaaatgaa ataaaagatc cccgtattaa agatacttta gtatctgttg tgaatgttga
    27301 tacatccagc gacctgaaaa tctgcaaaat atatattagc gtcatggggg ataaagaaca
    27361 aaaagagaat gttattgaag gtttaaaaaa tgccagtggt tttatccgca gggaaatagc
    27421 cagaagaata aatctcagaa atacgcctga attcattttt aaattagacg attccattga
    27481 atatagtatt catctttcca ggattatgga tgagataagc cataacagtg gagatgatac
    27541 tcatgactag tcaggagata tatcagctaa ttcatgattc taactatatt gctatagccg
    27601 ttcacacgaa tcccgatggt gatgctattg gagcgtgtac ggcgttagct ttggttctta
    27661 aaaaaatcgg caagaagcca attattctaa tgagagagat tcctgataaa tataactttt
    27721 tagcttatca tgaatttatt tgtcaggaat atccgaagca tttgcctctg gaacttttta
    27781 ttgctttgga ttgtggtgac aaagaaagaa taggagaata ttcgaatcat tttgatatag
    27841 cggatacgac aatgaatatt gatcaccata ttacaaatct tgaatatgct gattataatt
    27901 tagtaaattc taaggcatct tcaacttgtg aaatcattta taagcttata aaagattggg
    27961 aaatctcttt agatcaagaa atttcctctg ctttatatac agggatagct tttgacacag
    28021 gaggctttaa acataataat accagttctt atacccattt gattgttgca gacttgttag
    28081 cttataatat taattttacc gatataatgg acaaattgtt tcatacccgt tccatatctg
    28141 ccacaaagct acttggttat tctttaaaca aaatggaatt ttatcaaaac aatcaattgt
    28201 gcctttgtac gttagataaa aaagaacttg aatccttcgg ggctaaagac ggggatactg
    28261 aaggtattat atctttctta aaaaatacgg aaggtgtttt aatagcctgc ttgttatatg
    28321 aaaaaacaga aaatgaaaca aaggtaagtt tccgctcttc cggagatatc gatatttctt
    28381 atattgctca aaaatttgga ggtggcgggc ataaaaaagc tgcaggctgt accatcaaca
    28441 aaaatattaa tgaagcaaaa gaagaagtta ttaataccat tttaaaagaa ttgttttaag
    28501 gagataaggg aacttgaatg gcgttataaa tatttataaa gaaaaaggtt atacttctca
    28561 tgatgccgta gcggatgtca ggaaaatttt aaagcaaaaa agaatagggc atacagggac
    28621 ccttgaccct gaagctgaag gggtactgcc tgtctgtatc gggaaggcga ctaaagctgt
    28681 agagtattta atggatttta gaaaaaccta tcaggcaact cttgaatttg gaataattac
    28741 tagcactcaa gattatactg gagaaatttt aagtcaaaag aaagtagatt ttgacgaaga
    28801 aaagattaaa gaagccgttt tttcttttct gggagaatat gagcaaattc ctccaatgta
    28861 ttctgctatt aaagtaaacg gaaaaaggtt atatcaactt gccagggaag ggaaaacggt
    28921 ggaaagaaaa gcacgaaagg tatatatata tcatattgat attctttctt ttttaccgcc
    28981 taatcaaatt aaaattgaag tcgaatgctc aaaaggaact tatattagaa cgctgtgtga
    29041 agatataggt aataaattag gttttggtgc ttatatgaag gatttaatcc gtacaaaagt
    29101 aggcattttt gaaatagaaa acagtataaa aattaatgaa ttaagaagac aggttgagtt
    29161 gggcaatatc gataaaattc ttattaaaac ggacgacttg ttttatcaat acaatgctgt
    29221 gaaaatccag tattctggtg aaaaatacct taagaacggt aataagattc attattccta
    29281 tgtcgaacaa ttgacgaatg atataaaaat tggcgatatt gtaagagtct atagctataa
    29341 agatgaattt attggccttt atgaaatcag gagcaattat ttgaaaccac tgaaactatt
    29401 tttgtaaggg gtttaaccat gaaatattta accaatttag acaatcctct tgataagcca
    29461 agtgtaatta ctattggaaa ttttgatggt cttcatttgg ggcatagaaa attaattagt
    29521 actgttaaag aactggctct tgcatatcat tatgaatcca ttgttttttc tttttacccc
    29581 catccgcatg aggttttaaa aaaagatgat tttcctggta tgatttattc aagagaagaa
    29641 aaaagagaaa ggatcgaaga aaccggtatt gatacatata tcgaatatcc tttcacaaaa
    29701 gaatttgcaa gttattcacc agaagagttt gtagtgaata tacttataga aaaactccat
    29761 gcaaaaatca tagtgatagg tagcaacaat cgatttggaa aaaatcagga gggagatgtg
    29821 aactttttaa aagaaagaga agcaaagtgg gatataaaag tagttgaaat ctctccggtt
    29881 ttagataatt ctttaatcgt aagcagcagt cgcataagag aagaattaat gaaaggcaat
    29941 atagaaaaag ttaatgaatt attaacactt ccatatatta tcagcggaac cattgtcgaa
    30001 ggaagaaaaa ttgggcgcca acttggctat cctacagcca accttttgcc caaaaaagat
    30061 agattatatc ccccaaatgg ggtctattta acaaaaacaa aatataagaa taaaatttat
    30121 aatagtatta cgaatatagg ttataatcca accgtaaatg gtaaaaacag agtgatagag
    30181 acttatattg atgatttttg tgcaatggtg tatggagaaa agataaccat tgaattttac
    30241 aaatggttaa gagcagaaaa aaagttttcc agtttagatg agttagttca gcagttagat
    30301 ttagatgtaa aaacttgcag agaatatttt aattaaatat tttacttata atgatttaat
    30361 tttacttaaa tttaaatttt aagttta
//
LOCUS       DAOSUD010000018        29994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1045_length_29994_cov_25.594108, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000018 DAOSUD010000000
VERSION     DAOSUD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 29994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29994
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1045_length_29994_cov_25.594108"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLG49_03945"
     CDS             <1..939
                     /locus_tag="PLG49_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ16503.1"
                     /translation="GKIYFKEDLEKILRVELDKEKVVVSDIAVIQNKNRKYEVTIKHF
                     PCNGRRLCHKKIVPVVSHLLKRKMKLAFSGCNAIGKKDECIIKLVEEEKFKILTGVAK
                     EKKNSAVISGDSYSFMKLKDEQFLLALSDGMGSGSRASEESCAAIELLEDFMETGFDK
                     DIAIKMINSVLLLKSNEESFSTLDMAIIDLYSGIAEFIKIGAVSSFLKRDDEVEIIGS
                     SSLPVGILNHVDIDTSKKKLKSGDIIIMVTDGVLDSQKEVLEREKWLSQVIAGINNKN
                     PQYIADYILDIAKNQSKDKIQDDMTVLVARIWDKSL"
     gene            1009..1320
                     /locus_tag="PLG49_03950"
     CDS             1009..1320
                     /locus_tag="PLG49_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16504.1"
                     /translation="MKFYSNNRIILIENEDGGWYEKAIFLLNKDIHKEHQPMEIVREA
                     EKIIENYIRNNHYAANKNPTKRKSSLKSYKKRSTNRIINICLAISIIVFLYYLFLLIT
                     Y"
     gene            1443..2819
                     /gene="tilS"
                     /locus_tag="PLG49_03955"
     CDS             1443..2819
                     /gene="tilS"
                     /locus_tag="PLG49_03955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOQ16505.1"
                     /translation="MLKRIDHMIKKYNMINKNDKIIVGVSGGADSLCLLHLLKALSEA
                     YPFEITAVHVNHGIRGKEADDDENFVRNLCIQWGIPFKAYHVDIKEEAKRRKLSEEET
                     GRKIRYEIFESLRKELAADKIAVAHNMNDQAETVLMQLFRGTGMRGLAGIPPIRDNII
                     RPLLGFSREEIENYCRTNQIAFRQDSTNVLNIYTRNKIRNKIIPLIKEDFNPNIIENL
                     FNLSNLLREEDAYLEGIAEEAFQRCLKEKNSDSIVLDNNLFGTYNIVIQRRILRTVIE
                     ALLGHIKDIEYNHIRDILDLSQNQTGKRVYLPKGIIVKRQYGELIFQTGENQEISFCY
                     ELPVPGSLVIKEAGIMVEVKIIDKADFKLFNQNTYTKSFDYDKIKGNLQIRTRCEGDR
                     IFLDKGSKKLKKFFIDEKIPKDKRSQIPLLAEGNRILWIIGYRYSDAYKITSSTNRVL
                     EVKVMHIQ"
     gene            2858..3382
                     /gene="hpt"
                     /locus_tag="PLG49_03960"
     CDS             2858..3382
                     /gene="hpt"
                     /locus_tag="PLG49_03960"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOQ16506.1"
                     /translation="MMEHLDILISEEEIKKRVKELGKQITQDYRGKELCLICVLKGGV
                     MFMVDLAKEIDLLLEMDFMAVSSYSNEKTSSGVVKIIKDLDEPIEGKHVLIVEDIIDS
                     GRTLSYLVKILNDRKPASLRICTLLDKPEKRVTDVRVDYVGFTIEDKFVLGYGLDYMQ
                     KYRNIPYIAVLKDE"
     gene            3549..5462
                     /gene="ftsH"
                     /locus_tag="PLG49_03965"
     CDS             3549..5462
                     /gene="ftsH"
                     /locus_tag="PLG49_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ16507.1"
                     /translation="MLIMMLVLLLLFFGMSGLPKSPETRLTYNQLMDKLKNNEVERLE
                     IYPDADSNTANVEIILKNGDISKITVPNVGYFMERLEEYEESIEINTHDISRPSWFIQ
                     YLPGIIMLIVVIVLFIYLMQQLQSGNGGSRVMSFGKSRAKMAIDEKKRVTFDQVAGLD
                     EEKEELQEIVEFLKHPKKFVELGARIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFDQAKKNSPCIVFIDEIDAVGRRRGAGLGGGHDEREQT
                     LNQLLVEMDGFGVNEGVIVVAATNRPDILDPALLRPGRFDRQVVVGRPDVKGREEILK
                     VHAKGKPLADDVDLKVVAQTTSGFTGADLENLLNEAALISARDNKKYIDMANIQRALI
                     KIGMGTEKKSRVVSDKEKRLTAYHEAGHAIIFQVLPELDPVHSISIIPTGMAGGYTMP
                     LPSEDNLYMTKTKMEQEIVCFLGGRAAEEIVFGEITTGASNDIQRATALARNMVTKYG
                     MSSLGPIQFGDDNDEIFLGRDFVHARNYGEEIASAIDKEVKKIIDHAYEEAKRILLEN
                     IDILHKTVDLLLEKEKITGEEFAALFNEDSNKKDDEGNQDKDNQEIKDQKDNQEKQET
                     PDDNDELNLNSDA"
     gene            5535..6515
                     /locus_tag="PLG49_03970"
     CDS             5535..6515
                     /locus_tag="PLG49_03970"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOQ16508.1"
                     /translation="MKSKIVEILKEKNDYVSGEEMSNLLGVSRTAVWKTISRLKEEGY
                     VIEAVTNKGYYLKQSPDLLTEEEISPTLKTRYIGRKIYHYKQVDSTNKEAKKLAIDGA
                     AEGSVVISEEQTAGKGRLGRTWASPYGLGIWMSLILRPQITPVEVSKITLIAALAVSK
                     TIQKVTGLKAEIKWPNDILINKKKVCGILTEMSAEMDKINYIILGIGLNVNTEEFPEE
                     IAHKATSLKIEGKKSYDRKELTQELLFEIENYYRLFTKQETVDKLLEEYKACCVTLNK
                     EVKIIRNGIEYIGKAVGLTENGELLVEKEDGELIHVFSGEVSVRGIDAYV"
     gene            6557..6922
                     /locus_tag="PLG49_03975"
     CDS             6557..6922
                     /locus_tag="PLG49_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HOQ16509.1"
                     /translation="MDKKVICSASHYAQKYYFSPEFNGIPAAIKNEIKEICIITAEKL
                     RGIFTMGFYENGEIFFEVRSEGNDYNFDEIGAPLEIKRIELEKKELLKALKLWYKFFM
                     TEEGRTIIEKMDERNNAPS"
     gene            7074..7718
                     /locus_tag="PLG49_03980"
     CDS             7074..7718
                     /locus_tag="PLG49_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ16510.1"
                     /translation="MKETAIAKTSSTKKLVIAGMLVAISIILDTTPFGTIRLPIISAT
                     ISHIPTIIGAILCGPVVGAVVGLSFGVTSMIRNLTQPTSVLAFALKNPLVSVLPRALV
                     GIVSYYIYAGLKKVKPEKNKEEKVKRKKIIFEDIPIVVAAAIGSLTNTIGVLGMMYIL
                     YAEEIVAKFAEIGKSGTIYGILLGIASANGIPEAIVSVILTFAVVKSLKRIYNN"
     gene            7780..8547
                     /locus_tag="PLG49_03985"
     CDS             7780..8547
                     /locus_tag="PLG49_03985"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOQ16511.1"
                     /translation="MILAIDVGNTNIVLGAFKEDELVTHWRMTTNVHQTSDELGIFIY
                     NLLRHNNIEVEEIEHVVVASVVPDIMRSLEYGIKKYLKTAPLIIGPGIKTGINIRTDN
                     PKELGADRIVNAVAALYLYGSPVIVIDFGTATTFDVINEEGSYIGGVIAPGVNISAEA
                     LWQRAAKLPKIEIQKPNKIIGKNTVDSMQAGLFYGYVGQVDYIVKKIKRELNCTNVKV
                     VATGGLARLIAGESKTIEEVNDFISLQGLKIIYEKNK"
     gene            8556..9524
                     /gene="dusB"
                     /locus_tag="PLG49_03990"
     CDS             8556..9524
                     /gene="dusB"
                     /locus_tag="PLG49_03990"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOQ16512.1"
                     /translation="MKIGNVTIPNSVFLAPMAGVTDLPFRLLCKEMGCGLVYTEMVSA
                     KGLFYGNENTKLLLEIDKREHPVAVQLFGSDPEIMSEMAKKLEETDVDIIDINMGCPA
                     PKIVKNNEGSALMKEPKKVGEIIKAVSSSVKKPVTVKIRKGFDENHINAVEIAKIAEE
                     NGAAAIAVHGRTREQFYSGKADWNIIKQVKEAVSIPVIGNGDIFSPEDAKKLFEESHC
                     DAIMVGRGAQGNPWIFKRILHFFETGDILEEPSAEEKIKMALRHAKMLIEYKGEYIGI
                     REMRKHVSWYTKGLLNAGILRSKINHVNDYKELEFLLLEYLQEINH"
     gene            9637..10614
                     /locus_tag="PLG49_03995"
     CDS             9637..10614
                     /locus_tag="PLG49_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16513.1"
                     /translation="MKKFAYLKWAKNWEEIFNQMPWLKIFPSFLRENVIIHFCPLKLK
                     ERWIMDLPGFEVILPFTAEQVNKKAVQVIQDYINKVIERLKTEGVKIICPPKGHSHEI
                     DFSILEAKGQYVISLLIFEAIKKLSEKLEKPLKYIEITIIDGQNLRTDLVLNILYPEL
                     NYLSILTDKTESFREKAVDIYEDVGLNLQIISYNKKTVLENSDIIIDCRDVYHSSFYY
                     CKKNAIYIYIGGYKEMVRDILLKRKDLKIIDNVSLVLKNESYCADFCEMLWYLSKTWF
                     KNILSGNYDLALLQKVIKEIRKEGWVIGEFYQYGRILDINQFNHIDKFF"
     gene            10696..11172
                     /gene="greA"
                     /locus_tag="PLG49_04000"
     CDS             10696..11172
                     /gene="greA"
                     /locus_tag="PLG49_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOQ16514.1"
                     /translation="MTEKKIVLTYEGLRKLETELQDLKTVRRKEVAEKIKEARGQGDL
                     SENAEYDAAKEEQAEIETRIVELEKILRNAQVIDEDEVGVDEINIGCKVKIWDMELCQ
                     ELDYAIVGSTEADPSEGKISNESPVGAALMGHKVGDVVEVEVPEGKLEFKVISIYR"
     gene            11196..12698
                     /gene="lysS"
                     /locus_tag="PLG49_04005"
     CDS             11196..12698
                     /gene="lysS"
                     /locus_tag="PLG49_04005"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOQ16515.1"
                     /translation="MSEENQLQFENEEQDINELIRIRREKLKDLQGKGKDPFKLVKYE
                     VTHHSKDIHENFDELEGQTVSVAGRIMAKRVMGKASFCDVQDREGRIQIYVRKDSVGE
                     EFYEEFKHYDIGDLIGVKGEVFKTKKGEISVKANEIELHAKSLQILPEKWHGLRDTDL
                     RYRQRYLDLIVNPEVKDTFIKRSKIIKEIRRFLDDRGFLEVETPVLHTIPGGAAARPF
                     ITHHNALDMDMYMRIALELHLKRLIVGGMEKVYEIGRVFRNEGISIRHNPEFTLLELY
                     EAYTDYHGMMDLTEELIRTVAKNVLGTTKIPYGEYEIDLGKPFERLTMLDAVKKYANI
                     DFNEVHSTEEAKALAKEHNIEYQEHHKKGDILNLFFEEYVEKNLIQPTFVMDHPVEIS
                     PLAKRKPDNPDYTERFELFITTREFANAFSELNDPIDQRKRFEYQEALRAAGDEEANM
                     IDEDFLTALEYGMPPTGGLGIGIDRLVMLLTNSASIRDVLLFPTMKHLQK"
     gene            12925..14127
                     /locus_tag="PLG49_04010"
     CDS             12925..14127
                     /locus_tag="PLG49_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOQ16516.1"
                     /translation="MKKSRKIIIISVVALLLFIGIGSFFQHKNIKNGERKEDLASNEE
                     NMNKRISFDSYMSQPPVDYSVYLKPKKLKAIYVTGWKAGSSQYIDELIDIVNTTEINA
                     MVIDVKSDDGYITFDMDIPLAKEIGSTKNAGIRDIEGLMDKLRENNIYPIARVVVFKD
                     PYLAEKRPDLAIKNKDGSIYRSKGVAWANPYNKEVWDYIIDISKEAARVGFKEIQFDY
                     IRFEENLKVESADLGPLANEKSKMEIIVDFTEYAVKELKPLGVFVSADVFGTVITSRI
                     DAEIVGQDYVEMAKHLDYICPMVYPSHYAEGSFGLPYPDLQPYETVLRAMEASNEKLA
                     EIPEGEHRAIVRPWLQDFTATWIDHYQPYGAKQIREQIQATYDAGLDEWILWSPSNRY
                     SVGGLLPE"
     gene            14304..14762
                     /locus_tag="PLG49_04015"
     CDS             14304..14762
                     /locus_tag="PLG49_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16517.1"
                     /translation="MIDKEKIKLMSKLAIYEKNYGSIDEKMNGYYKSDYVYFQNWWAR
                     ISAVIAGFFIVGLFFLQKIFIEKIDIFDLNYKEYGIRIAAFILILMAVYSFISSYVNG
                     KKYDESQERIKQYMEMLKQLDEHKSLKQEEVYKENYESDLSNPGDDNSFL"
     gene            14878..15648
                     /locus_tag="PLG49_04020"
     CDS             14878..15648
                     /locus_tag="PLG49_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16518.1"
                     /translation="MFSIICTIISTQWAFLLIIFLVTTHILFASVETAILFFVFLMII
                     YLLYIRLFPKHSLLIIAMVLAYFFKLPYIVPLFGGLFLGPVSIIPVAIGTIIWYFIPN
                     IQTIVGMKAEEIMDMPNNLARIYMTVIQGIAKNHTAITSIIIFSVVILVVYIISRLAI
                     DYSWYIAIGIGAGISFIGFLLGILIVDAEINIFGMFFSILISMLLMFVIQFFHRVVDY
                     SRAESVQFEDDENYYYVKVIPKIILEKPKREIKRIIED"
     gene            15735..16382
                     /locus_tag="PLG49_04025"
     CDS             15735..16382
                     /locus_tag="PLG49_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOQ16519.1"
                     /translation="MDTKVIYFDNIWIEPPMDKILYRLGYRKGKTILNKEQKEEIEQL
                     IWEGVSLCKPKGAYVIVPVTAKDESSVCIGDYLIISKSLAQLLKNSDYVALMASTIGH
                     DIIKVINNEIKKGNSVKAVTFDSTASQIADKTVAWVIEAINKNLKREARKLTSFRYSP
                     GYGDLDLKYQEIFFKELNLEKLEMSLTETFMMIPEKSVTAICGVEKIEREEEVNE"
     gene            16375..18786
                     /locus_tag="PLG49_04030"
     CDS             16375..18786
                     /locus_tag="PLG49_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOQ16520.1"
                     /translation="MNRTEFYNQLKERILILDGATGTELQKRGMPKGVCPEDWILKNP
                     DSLIEIQNAYKEAGSDIIYAPTFGANRWKLETFGLEDKVKEINVNLVRLSKDIAGQEV
                     LVAGNLSATGKFIKPFGDKTFEEAVNIYKEQVEALLEGGADLFVIETMIDIQEARAAL
                     LAVKEICDLPVMVSMTFGEDKKTLTGTDPVTAVITLQALGADAIGCNCSTGPQDMIPI
                     IKEMKSIASVPVFAKPNAGLPVMKDGEFYYDLNPEDFASFLGDFVEAGINLIGGCCGT
                     NSEHIRLLSLKSKDYKPKEWLEHRISASSSVRKTLFIGRDTPLRIIGERINPTGKKDL
                     QKELLEGKLSLVRKFALEQQNKGADMLDVNVGMPGIDIKNTMIEVVENLSVISELPLC
                     IDSSEPEVIEAALRVYPGRALLNSISGEKKKLEKLLPVAAKYGAMFIALPLNDEGVPE
                     TAEKRIQIINEILEEAKKVGYGPEDMVVDGLVMTVSSDEKAAQETLKVVEYASSVLKT
                     STILGLSNISFGLPERSYLNSAFLLMAMGKGLNMAIANPANENMMNLKIAGDVLTGRD
                     RGCRFYIQHFNKASEEIKLQAGNFPEENIKSKIYNAVLKGEKEDIEEYIKMALKEENA
                     PYDLVDDILIPAINKVGELYDKKEYFLPQLILSAETMKKAFDYLEPLLLKENTGQKKT
                     KIILATVKGDIHDIGKNIVGLMLKNYGFDVIDLGKDVPKERIIEEIKKTGAPMVGLSA
                     LMTTTMGEMKHVIDAVKEEGLETKFIVGGAVVTREYAKEIGADGYAEDAMEAVKVSQL
                     LEKDL"
     gene            18886..19761
                     /gene="cdaA"
                     /locus_tag="PLG49_04035"
     CDS             18886..19761
                     /gene="cdaA"
                     /locus_tag="PLG49_04035"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOQ16521.1"
                     /translation="MNLNSLRDLVSQLKWSIMNIPSIRIGDIVDILIVAYLIYKIIIW
                     IKDTRAWALFKGVVVIFSAAVIAYVFQLNTVWWILSNTISVGIIAVFVVFQPEFRRAL
                     EQLGRGRFFSNFMIFDEQREREESISTGTIEAIIKAVEHLADSKIGALIVIERNVKLG
                     EYERTGIKIDALVTAQLLINIFQPNTPLHDGAVIIKNNRVSAATCYLPLTDSLEISKE
                     LGTRHRAALGISEVSDAITIVVSEETGIISVTDSGSLTRNIKGEFIRTMLKDHSSKKM
                     DRKKLVLWKGRHKNE"
     gene            19754..21055
                     /locus_tag="PLG49_04040"
     CDS             19754..21055
                     /locus_tag="PLG49_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOQ16522.1"
                     /translation="MNELLGKNLGWKVFSLILAIGLWFIVINIKNPEETMSFTIPVEI
                     QNLGVIEANDLIIVNFDEIMDKNITINLRGTRLTLDKLKSNKKYLDVIKASANLELYR
                     YTKATEVNKIPIEITFSEEYKGLIYEERHQIRYLDLILEERKSITKKIEAEIIGQISN
                     EYVVLEPRVNPGEITITGGESLINKINVVKVEVNAHDILDNKTVEVEPKIYDLQGNEI
                     TELSKNVKTAKVSAEIGKKRKITLVPNIQGSYAENHVSTGIKIEPSEIVIVGKEEIIN
                     NISQVQLSPIVFNDLRETTVFNPTIILPAGVSRLDSNESRVSVTIEVKRQVTKEFKIP
                     TSKLTLRTEKQFRYISKEVTLRLFGIEDDINRISESSLQGSIDVSNLGEGRHKVAIKF
                     NLPGNVKLVGDPPMVEIELIEGEEEGEGSDEEVEETAEEVS"
     gene            21236..22585
                     /gene="glmM"
                     /locus_tag="PLG49_04045"
     CDS             21236..22585
                     /gene="glmM"
                     /locus_tag="PLG49_04045"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOQ16523.1"
                     /translation="MGRLFGTDGVRGVANTELSIELAYKLGQAGAYVLSKEAHHQARI
                     LVGKDTRISGNMLEAALIAGICSVGAKAITVGVIPTPAVAYLTKKYNADAGVVISASH
                     NPVKYNGIKFFNGEGYKLSDALEEKIEAIILDNAEQLPAPIGEEVGTIIEKYDAISDY
                     VEFLKSCVDVDFKGLKVVVDCANGAAYKAGPRVLEELGAELIVIHNEPNGCNINKDCG
                     STHMENLCKAVVENKADLGIAFDGDADRCLAVDENGIIVDGDQIMSICGLQMKKDNQL
                     KKNTIVATVMSNLGFFIMAEKNNLKIEQTKVGDRYVLEEMLKHDYNLGGEQSGHIIFL
                     DHSTTGDGILTAVKLLSVVKKTGKKLSELAGVMEVLPQVLVNAKVPNDKKYSYMENDR
                     IKKAIEELEGKFAGEGRVLIRPSGTEPLVRVMIEGKDEQVLKEEAEKLAKIIEEELN"
     gene            22761..23912
                     /locus_tag="PLG49_04050"
     CDS             22761..23912
                     /locus_tag="PLG49_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16524.1"
                     /translation="MRDEFRNWMIQEDGIAEYIAYQYVTAIDKISEHYNEQTGANLDI
                     YKETDLKKLKEIANEYGPNGKYADFSSFGNGMLRNAIKAYIEFLCQKFGIKELEDYQF
                     SHFNFQDSFKAQDHFKAQDNLNVHDNFKAQDNFNVHDSLKTQDNFNVHDSFKVQDSLN
                     VHDSFKTQDNLNVHDNFKVQDNFNVHDNFNTHDHFKTQDFKDYSNEKSEDEDVKSFEA
                     EKIESHELNYYVYNPVNDEENNLYPVNTNVKAKDGFKDFSNSYEKDIQMSLIYKIDEL
                     FPGYRIFGKNKEGINYEINGRTIYLLLENPMENSLLVVEINAGRADYKTFGKVAMYIG
                     MLSKEFPTKFVKGLIISGEIDDSLVNATRITDRIKVKTYQMKLSLRNII"
     gene            24014..24412
                     /locus_tag="PLG49_04055"
     CDS             24014..24412
                     /locus_tag="PLG49_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOQ16525.1"
                     /translation="MVKWSDDYLIGVPEIDEQHKRLFEIAGRAYKVMQDEYSIDKYDQ
                     IIEIIEELKDYAVYHFKSEEEYLTKIQYKKFFSQKIAHDSFVEKINNIDYEKIDEEQD
                     KYLIEIMDFIVNWIVNHIIKLDKEIPNVPL"
     gene            24537..25073
                     /locus_tag="PLG49_04060"
     CDS             24537..25073
                     /locus_tag="PLG49_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ16526.1"
                     /translation="MFFDLLKKRRSIRKYINKKIEKEKVDMIIKSALMSPSSRGRRPW
                     EFVLVEDRDLLEKLSNCREHGSQFLKEAALAVVVLADPDKCDVWIEDASIASLIIQLT
                     AHSLDLGSCWIQVRERYKNEFQKTEDYIKELLDIPPDYCVESIIAIGYPGEEKEAHDE
                     NNLSMDKVHYNKYNIKYS"
     gene            complement(25116..25670)
                     /locus_tag="PLG49_04065"
     CDS             complement(25116..25670)
                     /locus_tag="PLG49_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HOQ16527.1"
                     /translation="MSKTGNYLLIDENIYQDILSNKISISSILEDPEYQNSENYLNIG
                     ASWDIIHYALCGEQWEGQAPYKYVVLGGEKINDEDVGYGPARYLSPTQIKETYEVIKD
                     IRQEDWIEKYNWDKILKDVLYSSATDNDSEEEFPEYNEIDFEELKKSLESTIIERSWY
                     VFCKIKTIFEKAIRDSKYVLFYLS"
     gene            25861..26718
                     /locus_tag="PLG49_04070"
     CDS             25861..26718
                     /locus_tag="PLG49_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ16528.1"
                     /translation="MKEKYILLKNNMKIPRLGQGTWFIGDNKAEEKDEIESIRLGIEL
                     GMTLIDTAEMYGSGAAERLIGKAIKGYDRSKLFIVSKVYPHNAGRRNIFNACENSLKR
                     LDVDYLDLYLLHWRGSIPLRETVECMEELVAQGKIRSWGVSNFDSSDMKELWSVPNGS
                     NCVINQVLYHLGSRGIEYDLVPWMKEHNVPVMAYSPLAQAGSLRRGLLENKVVREIAQ
                     NRGISPVQLLLAFVLSHDNMIAIPKSGNPKHTRENAEVMNIILTEEELKMLDKAFPAP
                     NYKTPLDII"
     gene            26769..27263
                     /locus_tag="PLG49_04075"
     CDS             26769..27263
                     /locus_tag="PLG49_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009982714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16529.1"
                     /translation="MIYFKEAVNKEDIREISKLARKIFEEYFTPMIGFEQVDYMLKKF
                     QSEEALSKQIRDGCKYFMVLEDNKLAGYFAIKPEENRLFLSKFYLDKEYRGLGIARKM
                     MEEIIKFSQSQALPAIYLTVNKKNERTINIYKKFGFEIIKSVETDIGQGFFMDDYIME
                     KKLS"
     gene            27454..28752
                     /locus_tag="PLG49_04080"
     CDS             27454..28752
                     /locus_tag="PLG49_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ16530.1"
                     /translation="MTKKFIIIFVLFFMMASSLFCYKNNRTNTSQTLELSDRVEASEI
                     KEEPIVKELAFYIGSHKWYIDRQEQNDMEEYKKIKPIIRDNTILFPIGVVLRKFSEDV
                     SWDPVGKTISATLNGESFNLSINEKKVSVGDEFFNLKDDIKVYGNQYYLSAEDTARIL
                     NLRFQVKNGVIAFVHQDNHLDEAINYYTDKYNEELSNIKDRTSIKSLKIPVLMYHLCL
                     TEAPPDELKGMAVTVKEFDAQLAALKKAGYEGISFEQYYNYRLYGASLPEKPVIITFD
                     DGYENNYTNAFPILKKHNMKATIFIVTSAVGQQGVQYPHMTWEQMKEMEDSGLVIIHN
                     HGSYHAAMDQISRSNMRKVALGGYTSIEHHMGTRQIYVFAYPYLRYNAVTIEELKQAG
                     IHMQVTNLGDWLREGTSMSDIKRIRISHGTSEQQLLNSIK"
     gene            complement(28961..>29994)
                     /locus_tag="PLG49_04085"
     CDS             complement(28961..>29994)
                     /locus_tag="PLG49_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16531.1"
                     /translation="NICLGGAIVMVIHLIGKIVLILLIIVLAVVFIVNICIIIMMKKE
                     KIYDTLIVKPHDFNSIPNINLNLINDLENKLYNQGFILENSYKMTTKQLDVNLYSKIF
                     IHKEFCTLAKILYSYSYKIQQIENEEIKTQVQKYTFELITRFHDGSYLVSRIEPEKSF
                     FDYDDFDNNNIVLTYKDMADFDDLLHNHFNKINELIHSKSLDNTILSKDYPNIMNEEN
                     KRILDKMADLKILKYKPDENHYEYTWSGVFKFLISYYKNLLSKKDKNNAFGYQTKQTI
                     TSKKVPAPLAALDYFFIFSFIMMLILSKNPARTQAQTIFRFSVLIISIIGKIITSILI
                     VNYKKKQEN"
BASE COUNT        10918 a         3816 c         6010 g         9250 t
ORIGIN      
        1 gggaaaatat attttaaaga ggatttagaa aaaattctaa gagtggaatt agataaagaa
       61 aaagtggtgg tttcggatat agcagttatt caaaataaaa atagaaaata tgaagtaacg
      121 attaagcatt tcccttgtaa tggaagacgc ttatgtcata aaaaaatagt tcctgttgta
      181 agtcatttac taaaaagaaa aatgaaatta gcctttagtg gctgtaatgc tattggcaaa
      241 aaagatgaat gtataattaa attagtcgaa gaagaaaaat ttaagatttt gactggagta
      301 gcaaaggaaa agaagaatag tgctgttata tctggtgaca gctattcttt tatgaaatta
      361 aaagatgaac aattcttact ggctttatct gatggcatgg gatctggcag cagggcttca
      421 gaagaaagct gtgcggcgat tgaacttttg gaggatttta tggaaacagg ctttgataaa
      481 gatatagcca taaaaatgat taattctgtc ttacttttaa aatccaatga agaatcattt
      541 tcaactttag atatggctat tatagactta tacagtggga ttgcagaatt tattaaaata
      601 ggtgctgtgt ccagtttctt aaaaagggat gatgaggttg aaataattgg ttcatcttct
      661 ttgccggttg gtattcttaa tcatgtggat attgatacat ccaagaaaaa attgaaaagt
      721 ggggacatta ttattatggt gacagatggt gttttggatt cccaaaaaga agttttagaa
      781 cgggaaaaat ggcttagcca ggttattgct ggtatcaata ataaaaatcc tcaatatatc
      841 gcagattata ttttagacat tgcgaaaaac caatcaaagg ataaaattca agatgatatg
      901 actgtcctgg ttgccaggat atgggacaaa tctttataaa ataatcatgc atagattcat
      961 catataagcg aaaactatcc ataagaatta agaaaatgga gggtatttat gaagttttat
     1021 tcaaataacc ggattattct tatagaaaat gaagatggag gttggtatga aaaagcaatc
     1081 tttcttctaa ataaagacat acataaagaa catcaaccta tggaaatcgt ccgggaagca
     1141 gaaaaaatta ttgaaaacta tatccgtaat aatcactatg ctgctaataa aaatccaacg
     1201 aaaaggaaaa gttctttgaa gtcttataaa aaaagaagca caaacaggat tattaatatt
     1261 tgtttggcga taagtattat cgtattttta tattatttgt ttctattaat cacttattaa
     1321 gatagaatga agtggtgtac aaaaaagtac atcacttttt gtttatttct tctaacttgc
     1381 ttttataatt tttgttctgt attatttaat tataaacttt gattttaata ggtgttattg
     1441 ttatgttaaa aagaatagac catatgatta aaaagtataa tatgattaac aaaaatgata
     1501 aaatcattgt aggagtctcc ggaggagcgg attctttatg ccttcttcat ttgttaaaag
     1561 cattatcgga ggcttatcct tttgaaataa cagctgttca tgtaaatcat ggtataagag
     1621 gtaaggaagc cgatgacgat gaaaattttg tccgtaattt atgcatacag tggggtattc
     1681 cttttaaagc ctatcatgtg gatataaaag aagaagccaa aagaagaaaa ttgagtgaag
     1741 aagaaacagg aagaaagatc agatatgaaa tttttgaaag tttaagaaaa gaacttgctg
     1801 cagataaaat tgctgttgca cataatatga atgaccaggc agagacagtt cttatgcaat
     1861 tatttcgcgg aactggtatg agaggattag cagggattcc accaatccgg gataatatta
     1921 taagaccgct tttgggtttt agcagagaag aaattgaaaa ctattgcaga acgaatcaaa
     1981 ttgcttttag acaagattca acgaatgtat taaatatata tacgaggaat aaaatacgta
     2041 ataagattat tccccttatt aaagaagatt ttaatcctaa tattatagaa aatttattta
     2101 atttgtctaa tctgctcaga gaagaagatg cctatctgga aggcatagca gaggaagctt
     2161 ttcaaagatg cctgaaagaa aaaaattcag atagtattgt actggataat aatttatttg
     2221 gaacatataa tattgttatt caaaggagaa ttttgagaac cgttatagaa gctttgttgg
     2281 ggcatataaa agacattgaa tataatcata tcagggatat tcttgattta tctcaaaatc
     2341 agacagggaa aagagtgtat ttaccaaaag gtatcattgt aaagagacaa tatggcgaat
     2401 taatctttca aactggtgaa aatcaggaaa tttcattttg ttatgagctt cctgttcctg
     2461 gcagcctagt cattaaagaa gcaggtataa tggtagaagt aaaaattata gataaggcag
     2521 attttaaact atttaatcag aacacctata ctaaaagttt tgattatgat aaaataaaag
     2581 gtaatttgca aataaggaca aggtgtgaag gggataggat ttttttagat aaaggcagta
     2641 aaaaattaaa aaaatttttt attgacgaaa aaatacctaa agataagcgg agtcagattc
     2701 cacttttagc agaaggtaac cgtattcttt ggattattgg ttatcgttac agtgatgctt
     2761 ataaaattac atcttcaacc aatagagttt tagaggtaaa agtaatgcat atccaataat
     2821 atttatcaga tcattttatt taacaaaaca ggaggaaatt atggaacact tagacatctt
     2881 gatatcagaa gaagaaataa aaaaacgtgt taaagaatta ggaaaacaaa ttacccagga
     2941 ctatagagga aaggaattgt gtcttatttg tgtcttaaag ggtggcgtta tgtttatggt
     3001 agatttggca aaagagatag atcttttact ggaaatggat tttatggctg tttcaagtta
     3061 tagcaatgaa aaaaccagca gtggcgttgt aaagattatt aaagatttag atgaaccgat
     3121 tgaaggaaaa catgtgttaa tcgtagaaga tattattgat tctggccgta cccttagtta
     3181 tctggtgaaa attttaaatg acaggaaacc agcaagtctt cgtatctgta ctttattgga
     3241 taaaccggaa aaaagagtga cggatgtaag agtggattat gttggtttta cgattgaaga
     3301 taagtttgtc ttaggttatg gtcttgatta tatgcaaaaa tatagaaata ttccttatat
     3361 agcagtttta aaagatgaat aagagaacat actttattta atcttcatag ctattgaaaa
     3421 tattcatgtt attcttgatt attgcatgga taaaagcgat atgttattat ggttaagtgg
     3481 ccttttatat cgtagcggcg aaaggaggaa gatttgtgaa taaattaaga ggaagttaca
     3541 gtttatatat gttaatcatg atgctggtac tcttactgct gttttttgga atgagcggct
     3601 tgccgaaaag tccggagacc agactgactt acaatcaatt gatggataag cttaaaaata
     3661 atgaagttga aagattagaa atatatccgg atgcagattc aaatactgct aatgtagaga
     3721 taatattaaa aaatggagat atatctaaaa ttacggttcc aaatgttgga tatttcatgg
     3781 aaagattaga agagtatgaa gaatctatag aaattaatac ccatgacatt tcacgtccta
     3841 gttggtttat ccagtatctg ccgggtatta ttatgttgat tgttgttatc gttctgttta
     3901 tatatcttat gcagcaactt caaagtggta atggcggaag cagagttatg tcttttggaa
     3961 aaagccgggc aaagatggct attgacgaaa agaagagagt tacttttgat caggtagcag
     4021 gtctggatga agaaaaagaa gaattacaag agatagttga atttttaaag catcctaaga
     4081 agtttgtgga actgggcgca agaataccaa aaggcgtctt attggtagga ccaccaggaa
     4141 ccggtaagac tttgctggct aaagcagttg cgggagaagc aggggttcct ttctttagta
     4201 tctctggttc tgactttgta gagatgtttg ttggtgtggg tgcttccaga gtaagggatc
     4261 tttttgacca ggccaaaaaa aattcacctt gtattgtatt cattgacgaa attgatgcag
     4321 ttggacgtag aagaggagca ggacttggcg gtggtcatga tgaaagagag caaactttaa
     4381 atcagcttct tgttgaaatg gatggatttg gagtaaatga aggcgttatt gttgtggctg
     4441 caaccaacag accagatatt ttggatccgg cgttattacg tcctggacgt tttgacagac
     4501 aagttgttgt tggaaggcca gatgttaaag gcagggaaga aatattgaag gttcatgcca
     4561 aaggaaagcc tttagcagat gatgttgatt tgaaggtagt tgcacaaact acatctggct
     4621 ttacaggtgc tgacctggaa aaccttctta acgaggcagc actcatttca gccagggaca
     4681 ataaaaagta cattgatatg gccaatattc aaagggcatt aataaaaata ggtatgggaa
     4741 cagaaaaaaa gagccgggta gtatctgata aagaaaaacg tttaacggca tatcacgaag
     4801 caggtcatgc aattattttc caggtattgc ctgagttaga tcctgtacac agtatctcta
     4861 ttattccgac ggggatggct ggaggttata ccatgcctct tccatcagaa gacaatctgt
     4921 atatgacaaa aacaaaaatg gagcaagaaa ttgtgtgttt tttaggtgga agagcagctg
     4981 aagaaattgt ttttggcgaa attacaacgg gtgcttctaa tgatatacaa agggcaacag
     5041 ctttggcaag aaatatggta accaaatatg gaatgagctc tcttggccca attcaatttg
     5101 gagatgacaa tgacgaaatc tttttaggca gggattttgt tcatgcaaga aattacggag
     5161 aagaaatagc gtctgcgatt gacaaagaag ttaaaaagat tattgatcat gcctacgaag
     5221 aggctaaaag aattcttttg gaaaacatcg atattcttca taaaaccgtt gatttattgt
     5281 tagaaaaaga aaaaatcaca ggcgaagaat ttgctgcttt atttaatgaa gattcaaata
     5341 agaaagatga tgagggcaat caagataaag ataatcaaga gatcaaagat caaaaagaca
     5401 atcaggaaaa acaagagact ccggatgata acgatgaatt aaacttgaat tcagatgctt
     5461 aagcttaaaa cactacagct tgtactgtag tgttttattg tgaactgatt ttttgggagg
     5521 atagctttat gagtctgaag agtaaaattg tagaaattct taaagaaaaa aatgactatg
     5581 tttcgggaga agaaatgagt aatttgttag gtgtatcaag aacggctgta tggaaaacga
     5641 tttcacgctt aaaagaagaa ggttatgtca tagaagcagt taccaataaa ggttattatc
     5701 taaaacagtc tccggattta ctgacagaag aagaaatatc tccaacttta aaaacaagat
     5761 atatagggag aaaaatatat cattacaaac aagtagattc aaccaacaaa gaagcaaaga
     5821 aattggccat agatggagca gcagaaggta gtgtagttat ttcagaggaa caaacagctg
     5881 gcaaaggaag actggggaga acctgggctt ctccttatgg gcttgggatt tggatgtctt
     5941 tgatattaag accacagata accccggttg aagtatctaa aattactttg attgctgcgc
     6001 ttgcagtgtc taagaccatt caaaaggtaa cgggcctgaa ggctgaaatt aaatggccca
     6061 acgatatact cattaataag aaaaaagtat gcggtatctt aacggaaatg agtgccgaaa
     6121 tggacaaaat aaattatatt attttaggta ttggcttaaa tgtgaataca gaagaatttc
     6181 ccgaagaaat tgcccacaag gccacatctt taaaaataga aggtaaaaaa agctatgaca
     6241 gaaaagaact gactcaggaa ttgctttttg aaatcgaaaa ttactataga ctctttacaa
     6301 agcaggaaac agttgataaa ttattggaag aatataaagc ctgctgtgtg acactcaata
     6361 aagaggtgaa aatcatccgt aacggcattg aatatattgg caaggcagtt ggtttaacag
     6421 aaaatggaga actattggta gaaaaagaag atggggagct tattcatgtt ttctcaggag
     6481 aagtctccgt aagaggtatc gatgcttatg tataaatgga gaatataaaa agtcagtata
     6541 aaatttggag gtcataatgg ataaaaaggt catttgttca gcttcacatt atgcgcaaaa
     6601 gtattacttc agtcctgaat ttaatggtat tcctgcagcc atcaaaaacg agattaaaga
     6661 aatttgtatt atcacagcag aaaagttgcg ggggattttt accatgggct tttatgaaaa
     6721 tggggaaatc ttttttgaag taagatctga aggaaatgat tataattttg atgaaatagg
     6781 tgctccttta gaaattaaaa gaattgaatt agaaaaaaaa gaattgttaa aagctctgaa
     6841 gctatggtat aaatttttta tgaccgaaga aggccggact atcattgaaa aaatggatga
     6901 aagaaataac gcgccaagct aattgaattt tataaaattt tagttataat attcttatag
     6961 ctgatattcc cttttaaaag tttcacaggt ttttaaattc gggaaatcag actataattt
     7021 tataaacaaa gaatacgtct tgtatccttt ggaacgagaa cggaggtgaa aaaatgaaag
     7081 aaacagcaat tgcaaagaca agttctacaa aaaagcttgt tattgcaggg atgttggtag
     7141 ctatttccat tattcttgat acaacaccct ttggaaccat ccgtttgccc ataatcagtg
     7201 caacgatttc tcatattcca acgattatcg gggctatttt atgtggtcct gtagtggggg
     7261 ctgttgtagg attatctttt ggcgttacca gtatgataag aaatttaacg caacctacat
     7321 cagtacttgc ttttgctcta aaaaatccct tagtttctgt gcttcccaga gccctggtag
     7381 gtatagtatc ttattacatt tatgctggtc ttaaaaaagt aaaaccagaa aagaataaag
     7441 aagaaaaagt gaaaaggaaa aaaataatat ttgaagatat tcctattgtc gtagctgctg
     7501 caattggaag tttaacaaac accatagggg ttttgggtat gatgtatata ctttatgcag
     7561 aagaaattgt tgcaaagttt gctgagattg ggaaatcggg aactatttat gggattttat
     7621 tgggaatagc gagtgccaac ggaataccag aggcaattgt ttctgtgata ttaacatttg
     7681 ccgttgtaaa atcactaaaa agaatatata ataattaatg cttattaagc agacactata
     7741 gtgtctgctt ttatagtaat cagcgaaaga gggaaaaata tgatattggc aattgatgtt
     7801 gggaatacaa atattgtttt aggtgccttt aaagaagatg aattagttac ccattggaga
     7861 atgacaacca atgttcatca gacttctgat gaattaggta tatttatata caatttactc
     7921 aggcataata atatagaagt agaggaaatc gaacatgtgg ttgtggcttc tgttgtgcca
     7981 gatattatgc ggtctttaga atatggtata aaaaaatact taaagactgc ccccttgatt
     8041 ataggacctg gtattaaaac aggtatcaat ataagaacag acaatccaaa agaattagga
     8101 gctgacagaa ttgttaatgc agtagcagcc ctttatttat atggcagtcc tgtcattgta
     8161 atagattttg ggacagctac tacttttgat gtcataaacg aagaaggaag ctatataggc
     8221 ggcgttattg ccccaggcgt taatatttca gccgaagcct tgtggcagcg ggctgcaaaa
     8281 ctccctaaga ttgaaataca aaagcctaat aaaattattg gaaaaaatac agtggatagt
     8341 atgcaggctg gtctttttta cggttatgtt ggtcaggtgg attatatagt taaaaaaata
     8401 aaaagagaat taaactgtac taatgtcaaa gtcgtggcaa caggtggtct ggcaaggtta
     8461 attgcagggg aatccaaaac gattgaagaa gtgaatgatt ttatatcctt acaaggactt
     8521 aaaataattt atgaaaaaaa taagtaggtg tttttatgaa gataggaaat gtaacgattc
     8581 ctaattcagt ttttttagct cccatggctg gtgttacaga cttacctttt cgcttactct
     8641 gtaaagaaat gggctgtggt ttagtatata cggaaatggt tagtgcaaaa ggtttatttt
     8701 atggcaatga aaatacaaaa cttcttcttg agatagataa aagagagcat cctgtagcgg
     8761 ttcaactttt tggttctgat cctgaaatta tgtctgaaat ggcaaaaaaa ctcgaagaga
     8821 cagatgttga tatcattgat atcaatatgg gatgcccggc gcccaaaatc gtaaaaaaca
     8881 atgaaggttc tgcccttatg aaagaaccaa aaaaagttgg ggaaatcata aaagccgttt
     8941 cttcttctgt aaaaaagcca gttacggtta aaatcaggaa gggctttgat gaaaatcata
     9001 tcaatgcagt tgaaatagcc aaaatagctg aagaaaatgg tgctgcagcc atagccgttc
     9061 atggaaggac aagagaacaa ttctatagtg gtaaagcaga ttggaatatt ataaaacaag
     9121 tcaaagaagc agtatcgata cccgttatag gcaatggaga tatattttct cctgaagatg
     9181 ctaaaaaatt atttgaggaa agccattgtg atgccattat ggttggaaga ggtgctcagg
     9241 gtaacccatg gattttcaaa agaatccttc atttttttga gacaggggac atattagaag
     9301 aaccctcagc tgaggaaaag ataaagatgg ctttaagaca cgctaagatg cttatagaat
     9361 ataaaggaga atatattggt ataagagaaa tgagaaaaca tgtttcatgg tatacaaaag
     9421 gtcttttaaa tgcaggaatt ttaagaagta aaattaatca tgtgaatgat tataaagaat
     9481 tagaatttct tttactggaa tatctccagg aaatcaatca ttaacaagaa ggtgtatgat
     9541 acaccttttt tcatttatca taattaagca actttcttca tacaatctta taaacttaat
     9601 atgattgtca atgagacaaa agtaggtgaa gattaaatga agaaatttgc ttatttaaaa
     9661 tgggccaaaa actgggaaga aatatttaat caaatgccat ggttgaaaat ttttccatca
     9721 tttctaagag aaaacgttat tattcatttt tgtcctctta agctgaaaga acgatggatt
     9781 atggatttac caggttttga agtgatttta ccgtttacag cagaacaggt caataaaaaa
     9841 gcagttcaag tcattcagga ctacatcaat aaggtcatag aaagattaaa aacagaggga
     9901 gtaaaaatta tttgcccgcc taaaggacat agtcatgaaa ttgacttttc aattcttgaa
     9961 gcaaaaggcc agtatgttat ttctctttta attttcgaag cgataaaaaa gctttcagaa
    10021 aagctagaaa agcctttaaa atatatagaa attactataa tagacggaca aaatttaaga
    10081 accgacttag ttctcaatat tctttatcct gaactaaact atttaagtat tttgacggat
    10141 aaaacagaaa gttttaggga aaaagcagta gatatctatg aagatgtagg attaaatttg
    10201 caaattatat catataataa aaaaacagta ttagaaaact cggatattat cattgactgt
    10261 agagatgtat atcatagcag tttttactat tgtaaaaaaa acgccatata tatttatatt
    10321 ggtggttata aggagatggt cagggatatt cttttaaaac gcaaagattt aaagattata
    10381 gataatgttt ccttagtact gaaaaatgaa agctactgtg cggatttttg tgaaatgctc
    10441 tggtaccttt caaaaacatg gtttaaaaac attttatcag gaaattatga tttagcatta
    10501 ttacaaaagg taattaaaga aatccgtaag gaaggatggg ttattggtga attttaccaa
    10561 tacggcagga ttctagatat aaatcaattt aatcatattg acaagttttt ttaggtgcgc
    10621 tataatccat aggatatgga gataatgcca gcttaatcat gtcataaaat aattgtagtg
    10681 gaaaggaagt tgtttatgac tgaaaagaaa attgttttaa cttatgaagg gctgcgaaaa
    10741 ttagaaaccg agcttcagga tttaaaaacc gttcgtagaa aagaagtagc agaaaaaatt
    10801 aaagaagcaa gaggacaagg agacttatca gaaaatgctg aatatgatgc agccaaggaa
    10861 gaacaagcag aaattgagac aagaattgta gaacttgaaa aaatactccg taatgctcaa
    10921 gttatcgatg aagacgaggt aggagtagat gaaataaata ttggatgtaa agttaagata
    10981 tgggacatgg aattgtgcca agagttagat tatgctattg ttggttcaac agaagcagac
    11041 ccttcagaag gaaaaatttc taatgaatct cctgtaggtg ctgcattaat gggtcataaa
    11101 gtaggcgatg ttgttgaagt tgaagtgcct gaaggaaaac ttgaatttaa agtaattagt
    11161 atatacagat aatatacgct aacggaggtg ccaaaatgtc agaagaaaat caacttcaat
    11221 tcgagaatga agaacaagat attaatgagt tgataagaat tagaagagaa aaactaaaag
    11281 atttgcaggg aaaggggaaa gaccctttca aactggtaaa atacgaagta acccatcata
    11341 gtaaagatat tcatgaaaat tttgatgaat tagaaggaca aactgtatct gtggctggcc
    11401 gtattatggc taaaagggtg atgggtaagg cttctttttg tgatgtgcag gacagagaag
    11461 gaagaattca gatatatgta agaaaagaca gtgttggtga agaattttat gaagaattca
    11521 agcattatga tataggggac ttaataggcg ttaaaggaga agtttttaag acaaaaaaag
    11581 gtgaaatttc tgttaaagcc aatgaaatag aacttcatgc aaagagtctc cagatcttac
    11641 ctgaaaaatg gcatggttta agagatactg atttgcgtta caggcaaaga tatttagatt
    11701 taattgttaa tcctgaagta aaagatacat ttattaaaag aagtaaaatt ataaaagaaa
    11761 tcagaaggtt tttagatgac agaggatttt tagaagtaga aactccagta ttgcatacca
    11821 tcccgggagg tgctgcagca aggcctttta tcacgcatca taatgctctg gatatggaca
    11881 tgtatatgag aattgctctg gaattgcatt taaagcgcct gattgtcggt ggcatggaaa
    11941 aagtatacga aattggtcgg gtgtttagaa atgaaggaat ttctattcgt cataatccgg
    12001 agtttactct tttagaactt tatgaagctt atacggatta tcatggaatg atggatttaa
    12061 ccgaagaatt aatccgtacc gtagctaaaa acgtacttgg aacaacgaag attccttatg
    12121 gtgaatatga aattgattta ggcaagcctt ttgaaagatt gactatgtta gatgctgtta
    12181 aaaaatatgc taatattgac tttaatgaag ttcattcaac agaagaagca aaggccctgg
    12241 caaaagagca taatattgaa tatcaggagc atcataaaaa aggagatatt ctcaatttat
    12301 tcttcgagga atatgttgag aagaatttaa ttcagccgac ctttgtgatg gatcatcctg
    12361 tagaaatctc acctttagct aaaagaaaac cggataatcc ggattatacg gaaagatttg
    12421 aactttttat tacgacaaga gaatttgcca atgctttttc tgaattaaat gatccaatag
    12481 atcaaaggaa aagatttgag tatcaggaag ccttaagagc agcaggtgat gaagaagcca
    12541 atatgattga tgaagacttt ttaactgcgc tggaatatgg tatgccgcct acaggaggtt
    12601 taggaatagg aatagatcgc ctagtgatgc ttttgaccaa ttccgcatca attagagatg
    12661 tacttttatt ccctacgatg aaacatcttc agaaataatg ggaacacttt cttttataaa
    12721 gaaagtgttt tcttctttat acagaaattg tttagtgaat tgacttttga aatatattgc
    12781 atggaaatac tttaattttt aatttacaat aattgaagca aaataattga gaaggacaga
    12841 tttagtaaga gtaaaaattg ctgtcgaaac tggaagtaat atatggtata ataaagaaca
    12901 aagaatatag aaggtggaga caggatgaag aagtcaagaa aaataatcat tatttcagtt
    12961 gttgccctgc tgttattcat tgggattgga tcgttttttc aacataaaaa tataaaaaat
    13021 ggtgaaagaa aagaggattt ggcctcaaat gaagaaaata tgaataaacg tatatctttt
    13081 gacagttata tgtctcagcc tcctgttgat tattctgttt atttaaaacc caaaaagctt
    13141 aaagcaattt atgtgactgg ttggaaagca ggttcatccc aatatattga tgaattaata
    13201 gacatcgtaa ataccacaga aatcaatgca atggtaatcg atgtgaaaag tgatgatggt
    13261 tatattactt ttgatatgga catacctttg gcaaaggaaa ttggttcaac aaaaaatgca
    13321 gggattaggg atatagaagg attaatggat aagttaagag aaaacaatat ttatcccatt
    13381 gcccgggtcg tggtatttaa agatccatat ttagcagaaa aaagaccgga tttggcaatt
    13441 aaaaataaag atggcagcat ttatcgttct aaaggtgttg cctgggccaa tccatataat
    13501 aaagaagtat gggattatat cattgatatt tctaaagaag ctgccagagt tggttttaaa
    13561 gaaatacaat ttgactacat ccgttttgaa gaaaatttaa aagtagaatc agcagatttg
    13621 ggacctttag ccaatgaaaa atcaaaaatg gagattatcg tggattttac agaatatgct
    13681 gtaaaagaat taaaaccact tggcgttttt gtttctgcgg acgtatttgg tacggttatc
    13741 accagtagga ttgatgcaga aattgttggg caggattatg tggaaatggc aaaacatctg
    13801 gattacattt gtcctatggt atatccttcc cattatgcag aaggttcttt tgggcttccc
    13861 tatccggatt tacagcctta tgagacagtt ttaagagcaa tggaggcgtc taatgaaaaa
    13921 ttggcagaga ttccagaagg ggaacatcgg gcaattgtaa gaccatggct tcaagacttt
    13981 acagccacct ggatcgatca ttaccaacct tatggggcaa aacaaataag agaacaaatt
    14041 caggcaactt atgatgctgg gttagatgag tggattttgt ggagtccttc caacagatat
    14101 tcagtagggg ggcttttgcc tgaataagta taaatagcgt gccattgccg cacgctattc
    14161 ttgtatacaa tatttttgca gagaaaatta aaaaatggaa caacttttct attattatga
    14221 tataatagaa aaggtcaaag aggacttttt ttactaaaaa atggtttata ataacaaaac
    14281 cctatacaaa ggagggattt ttattgattg ataaagaaaa gataaaattg atgtcaaaat
    14341 tggctattta tgaaaaaaac tatggtagta ttgatgaaaa aatgaatggt tattataaat
    14401 cggattatgt atattttcaa aactggtggg ccaggatatc agctgtcata gcaggatttt
    14461 ttattgtagg tttgtttttt ttacaaaaaa tatttattga aaaaattgat atttttgact
    14521 taaattataa ggaatatggt atacgtatag ctgcttttat tttaatttta atggctgtgt
    14581 attctttcat atcgtcttat gtcaatggta aaaaatatga tgaatctcaa gaaagaatta
    14641 agcaatacat ggaaatgtta aagcaattgg atgagcataa atctttaaaa caagaggagg
    14701 tttataaaga gaattatgaa tcagatttat caaatccggg agacgataat agctttttat
    14761 aaaagcaaag agagaatcat ttttccagtt ttaaaatttt tagcgtggtt tataattttt
    14821 caatttatta atattatagg atatgctcaa cctcttaagg ggatacatat agctcttttg
    14881 tttagcatta tatgtacgat tatttctaca cagtgggctt ttttattgat tatttttctt
    14941 gtaacgacgc atatactttt tgcttctgtt gaaacagcca tattattttt tgttttctta
    15001 atgattattt atcttttata tatccgctta ttccccaaac acagtttatt gattattgca
    15061 atggtcttag cttatttctt taagctgccg tatattgttc ctctttttgg aggtttattt
    15121 ctgggtccgg taagtatcat cccagtggcc attggaacga ttatttggta ctttataccg
    15181 aatatccaaa ccattgtcgg aatgaaagct gaagaaatca tggatatgcc taataatctg
    15241 gcaagaattt atatgacggt tatacaaggg atcgcaaaaa atcacacagc aattacttca
    15301 ataatcattt tttcagttgt gattctggtg gtatatatta ttagcaggct ggccatagat
    15361 tattcctggt atattgcaat tggaatagga gcaggaataa gtttcattgg cttcttatta
    15421 ggaattttaa tcgtagatgc agaaatcaat atatttggta tgtttttctc gattcttatt
    15481 tctatgttgc tgatgtttgt tattcaattc ttccataggg tggtggatta ttccagggca
    15541 gaatccgttc agtttgaaga tgacgagaat tattactatg taaaagtaat tccaaaaatt
    15601 atactggaaa aacctaaaag agaaattaaa agaataatag aagattaacc gtagaatatt
    15661 tttctacggt tttttattgc acgattgtat aaactgtggt ataaattcaa taaaactgtt
    15721 tggaagtgat taccttggat actaaagtca tctactttga caatatatgg atagaaccac
    15781 ccatggataa aattttgtac cggctgggct atagaaaagg gaagacgata ttaaacaagg
    15841 aacaaaaaga agaaatagag caattgattt gggagggtgt aagtctttgt aaaccgaaag
    15901 gggcctatgt aatcgtgccg gttactgcta aagacgagag cagcgtgtgt attggggatt
    15961 atcttattat cagcaaaagt ctggcacaat tattaaaaaa cagtgactat gttgctttaa
    16021 tggcatctac catcggtcat gatatcataa aagtgatcaa taatgaaatc aaaaagggta
    16081 attctgtaaa agcagtgacg tttgattcga ccgcatctca gatcgctgat aaaaccgttg
    16141 cctgggttat agaagccatt aataaaaatt taaaaagaga agcaaggaaa ttaacttctt
    16201 ttagatacag tcctggttat ggggatttgg acttgaaata tcaggagatt ttttttaaag
    16261 aattaaatct ggaaaagcta gagatgagtc ttactgagac ttttatgatg attccggaaa
    16321 aatctgtgac agccatatgt ggggttgaaa aaattgaaag agaggaagaa gtcaatgaat
    16381 agaacagaat tttataacca attaaaagaa agaatactca ttttagacgg tgctacaggg
    16441 acagaactgc aaaaacgggg tatgcctaag ggggtctgtc ctgaagattg gatattaaaa
    16501 aatcctgatt ctttaataga aattcaaaat gcttataaag aagcgggttc agatatcatt
    16561 tatgcaccta ccttcggagc aaaccgatgg aaattggaaa catttggatt agaagacaag
    16621 gtaaaagaaa tcaatgtgaa tttggtaaga ttatcaaaag acatagcagg ccaggaagtt
    16681 ttagtagcag gaaatctttc ggctacagga aaatttataa aaccttttgg agacaaaact
    16741 tttgaagaag ccgtaaatat ctataaggaa caggtagaag cccttcttga aggaggcgca
    16801 gacttattcg tcatagaaac tatgattgac attcaagaag caagggcagc ccttttagcg
    16861 gtaaaagaaa tatgcgattt acccgtaatg gtaagtatga cttttggaga agataaaaaa
    16921 actttgacag ggacggatcc agttacagcc gttattaccc ttcaggcgct gggggctgat
    16981 gctataggct gtaattgttc tacgggacct caggatatga ttcctattat taaagaaatg
    17041 aaaagcattg ccagtgtccc tgttttcgca aaacccaatg cgggtttacc cgttatgaaa
    17101 gatggggaat tttattatga cttaaatcca gaggactttg cttctttttt aggtgatttt
    17161 gttgaggcag ggattaacct cattgggggt tgttgtggta caaattctga acacatcaga
    17221 ttattaagtt taaaatctaa agattataag cctaaagaat ggctggaaca tagaattagt
    17281 gcaagcagtt ctgtcagaaa aactttattt ataggcagag acacgccctt aaggattatc
    17341 ggtgaaagaa ttaaccccac ggggaaaaaa gacttacaaa aagaactttt agaaggtaaa
    17401 ttgtccctgg ttagaaaatt tgccttagag cagcaaaata agggtgcgga tatgctggat
    17461 gttaatgtag gtatgccagg tattgatata aaaaatacca tgatagaagt ggttgaaaac
    17521 ttgtctgtta tttctgaact tcctttatgc atcgattcat cagagcctga agtgatagaa
    17581 gcggcactta gggtatatcc tggcagagca ttattaaatt ctatatcagg tgaaaagaaa
    17641 aaacttgaaa aacttcttcc tgttgctgct aagtacggag ctatgtttat tgcattgcct
    17701 ttaaatgatg aaggtgtccc ggaaacagca gaaaaaagaa tacaaattat taatgaaata
    17761 ttagaagaag ctaaaaaagt aggctatgga ccagaagata tggttgtaga tggtttagta
    17821 atgacagttt cttctgatga aaaagctgca caggagacct taaaagttgt tgagtatgct
    17881 tcttctgttt taaaaacatc aactatttta ggtttatcca atatatcttt tggattgcct
    17941 gaaagaagtt atttaaattc agcttttctt ttgatggcta tggggaaagg acttaatatg
    18001 gccatagcga atcctgccaa tgaaaacatg atgaatttaa aaattgcagg agatgtgttg
    18061 actggcagag acagaggatg cagattttat atacagcact tcaataaagc atcagaggaa
    18121 ataaaattac aagcaggaaa tttcccagaa gaaaatatta aatctaaaat ttataatgct
    18181 gttctaaaag gagagaagga agatatagaa gagtatatta aaatggcact taaagaagaa
    18241 aatgctccat atgatcttgt tgatgatata ttaattcctg ctattaataa agtaggagag
    18301 ttatacgata aaaaagagta ttttctccct cagcttattt taagtgcgga aacgatgaaa
    18361 aaagcctttg attatttgga acctcttctt ctaaaagaaa atacaggaca gaaaaagact
    18421 aagattattc tggcaacggt taaaggggat atacacgata taggtaagaa tattgttggt
    18481 ttaatgttaa aaaattatgg ttttgatgtc attgatcttg gaaaagatgt tcctaaagaa
    18541 agaataatag aagaaatcaa aaagacagga gcaccaatgg ttggtttatc ggcccttatg
    18601 actacgacaa tgggagaaat gaagcatgtt attgatgcag ttaaggaaga aggactggaa
    18661 actaaattta ttgtcggtgg ggctgttgta acaagagaat atgctaagga aataggtgca
    18721 gacggctatg ctgaagacgc gatggaagcc gtaaaagtat cacaattact tgaaaaagat
    18781 ttataaataa aagtatttat taccaggcag aataaaagag aaaaaaacta acaaaatcca
    18841 atggaacatg ctataattat ttataagaat ataaaaagag gagtaatgaa tttgaactct
    18901 ttaagggatc tagtttcaca attaaaatgg tctattatga atattcccag catacgtata
    18961 ggggatattg ttgatatatt gattgttgcg tatctcatct acaaaattat catatggatt
    19021 aaagacacca gagcatgggc cttatttaaa ggtgttgttg tcattttctc tgctgctgtt
    19081 attgcctatg tatttcaatt aaatacagtc tggtggattt tatccaatac aatttcggtt
    19141 ggtattattg ctgtttttgt agtttttcag ccagagttta gaagagccct ggaacaattg
    19201 ggtaggggtc gttttttctc taattttatg atttttgatg agcaaagaga aagagaagaa
    19261 agtatttcga ccggtaccat tgaagccatc attaaagctg tagagcattt agctgacagc
    19321 aagataggcg ctctgattgt tatagagaga aatgtgaagt taggggaata tgaaagaacc
    19381 ggaataaaaa ttgacgcatt agttacggcg caattgctga ttaatatttt tcaaccaaat
    19441 acccctcttc acgatggggc agtgattatt aaaaataacc gggtttccgc tgcaacctgt
    19501 tatcttcctt taacagatag tttagaaatc agcaaagaat taggaaccag gcacagggca
    19561 gctcttggta ttagtgaagt ttcagatgca attactattg ttgtatctga agaaacaggt
    19621 attatatccg taactgattc tggctcttta acaagaaata ttaaaggtga attcataaga
    19681 accatgttga aagatcattc gagcaagaag atggatagga aaaaacttgt gttatggaag
    19741 ggtagacata agaatgaatg aattattagg gaaaaattta ggttggaaag ttttttcact
    19801 tattctggcc attggtttat ggtttattgt tattaatatt aaaaatcctg aagaaaccat
    19861 gtcttttacg atacctgttg aaattcaaaa tttaggtgta atagaagcca atgatcttat
    19921 tatagtgaat tttgatgaaa tcatggataa aaacataaca attaatcttc ggggaacccg
    19981 cttgactttg gataaactaa aaagtaacaa aaagtatctg gatgttatta aggcttcagc
    20041 taatcttgaa ttatacagat acacaaaagc aacagaagtg aacaagattc ctattgaaat
    20101 tactttttca gaagagtaca aaggattgat ctatgaagaa aggcatcaaa ttcgttattt
    20161 ggatttaata ctggaagaaa gaaaaagtat tacgaaaaag attgaagcag aaattattgg
    20221 tcaaatcagt aatgaatatg ttgttttaga acccagagtt aatccagggg aaattacgat
    20281 tacaggcgga gaatcattga ttaataaaat aaatgttgta aaggttgaag taaatgccca
    20341 tgatatatta gacaataaga cagttgaggt tgagcctaaa atatatgatt tacagggcaa
    20401 tgaaattact gaactcagca aaaatgttaa aacagcaaag gtttctgctg aaataggtaa
    20461 aaaacgaaaa attactctgg tgcctaatat tcaaggcagc tatgcagaaa accatgtatc
    20521 tacaggtata aaaattgaac cttcggaaat cgttattgta gggaaagaag aaattattaa
    20581 taatatcagc caggtacagc tttcaccgat tgtttttaac gatttaaggg aaacaacagt
    20641 atttaatcct acaataatac tccctgccgg ggtatcacga ctggatagca atgaaagcag
    20701 ggtttcagta accatagaag ttaaaagaca agtaactaag gaatttaaga ttccgacatc
    20761 aaagttaacc ctccggacag agaaacaatt cagatatatt tcaaaggaag tgactttgcg
    20821 tcttttcggc atagaagatg acataaacag aatatccgaa agttctcttc aaggcagtat
    20881 agatgtttct aatttaggag aaggtcgtca taaagttgct attaaattta atcttccagg
    20941 taacgttaag ctggttggcg atccacctat ggttgaaatt gaattaatcg aaggtgaaga
    21001 agaaggcgaa ggcagtgacg aagaagtgga agaaacggcg gaagaagtat cttgaatttt
    21061 ttcaagatac tttttttaca gcttaggata ttttattttt taataaaact tttactctct
    21121 tataaaaagg tactattttt tgtaagtttt ttattatatt ttcttgttag tagctactag
    21181 ataatgatat aatgtgattt gtaaaaaata aaaactaaaa agaaaggcga ttagtatggg
    21241 aagattattt ggtacggatg gtgtccgtgg agttgcaaac acagaacttt caatagaatt
    21301 agcatataaa ttagggcagg ccggagctta tgtgttaagt aaagaagcac atcatcaggc
    21361 cagaatttta gtaggaaaag ataccagaat atcaggaaac atgcttgaag cagctttaat
    21421 tgcaggtata tgctctgttg gagctaaggc aattacggta ggtgttatac cgacaccagc
    21481 tgtggcatac ctgacaaaaa aatataatgc cgatgcaggt gttgtaatat ctgcttctca
    21541 caatccagta aaatataatg gtataaaatt ttttaatggt gagggctata aattaagtga
    21601 tgcactcgaa gaaaagattg aggcaattat attagataat gctgagcaat tacctgctcc
    21661 cataggagaa gaagtgggaa cgattattga aaaatatgat gccataagtg attatgttga
    21721 atttttaaaa tcctgtgttg atgtagattt taaaggtcta aaagttgttg tggattgtgc
    21781 taatggagct gcttataaag caggccccag ggtattagaa gaattaggag cagaattaat
    21841 tgtcatccat aatgagccaa acggttgtaa tataaataag gattgcggtt cgactcatat
    21901 ggaaaatctt tgcaaagccg tagtagaaaa taaggcagat ttaggaattg cctttgacgg
    21961 agatgcagac agatgtctgg ctgtcgatga aaatggcatc atcgtagatg gagaccaaat
    22021 tatgtcgatt tgtggtcttc aaatgaaaaa agacaatcag cttaaaaaga atacgattgt
    22081 agcgacggtt atgagtaatc tgggattctt tattatggca gagaaaaata atcttaaaat
    22141 tgaacaaacc aaagtagggg accgttatgt attagaagag atgttaaagc atgattataa
    22201 tttaggtggg gaacagtcag gacatattat tttcctggat catagcacaa ctggggatgg
    22261 aatacttact gcagtaaaac ttttatctgt cgttaaaaaa acagggaaaa agctttctga
    22321 attagccggg gtgatggaag ttttaccgca ggttttagta aatgccaaag ttcctaacga
    22381 taaaaagtat agttatatgg aaaatgacag aattaaaaag gctattgaag aattagaagg
    22441 aaaatttgcc ggagaaggca gagtgttaat ccggccatcc gggacagaac ctttagtacg
    22501 agtaatgatc gaaggaaaag atgagcaagt acttaaggaa gaagcagaaa agcttgctaa
    22561 aattattgaa gaagaactaa attaataatt tgattgaata gtatgcatta aaagtcgtgg
    22621 acaataataa aaaatttatt attgtctgcg actttttatt tttttgtaaa aaaattgtgt
    22681 aatttgtata taaatatgct aaaatataga atataaaaac aaatcggcca atacttctaa
    22741 aaaaatggag gtagagattt atgagagatg agtttagaaa ttggatgatt caagaggatg
    22801 gaatcgcaga atatattgcc tatcaatatg tcacggcaat tgacaagatt tcagaacatt
    22861 ataatgagca gacaggggct aacttagata tttataaaga aactgattta aaaaaattaa
    22921 aagaaattgc taatgaatat ggtcctaatg gaaaatatgc ggattttagc agctttggta
    22981 atggaatgct tcgaaatgcc ataaaagcct atattgaatt tttgtgtcag aagtttggaa
    23041 taaaagaatt agaggattat caatttagtc attttaattt tcaggacagc tttaaggctc
    23101 aagatcattt caaagctcaa gataacttga atgttcacga taatttcaaa gcacaggata
    23161 actttaatgt tcatgatagt ttaaaaacgc aggataactt taatgttcat gatagtttca
    23221 aagtacagga tagcttaaat gttcatgata gtttcaaaac acaggataac ttgaatgttc
    23281 acgataattt caaagtacag gataacttta atgttcatga taattttaat actcatgatc
    23341 attttaaaac tcaggatttt aaagattaca gtaatgaaaa atcagaagat gaggatgtta
    23401 aaagttttga agctgaaaaa attgaaagtc atgaattaaa ttattatgtg tataatccag
    23461 tgaatgatga agaaaataat ttatatcccg ttaataccaa tgtcaaagct aaagatggat
    23521 ttaaagattt ttccaactct tatgaaaaag atatacaaat gtctttaata tataaaatag
    23581 atgaactttt tcccggctat aggatttttg gcaaaaataa agaaggtata aattatgaaa
    23641 ttaacggtag aaccatctat ttgttattag aaaatcctat ggaaaatagt ttattagtag
    23701 tagaaatcaa tgctggcaga gcagattata aaacttttgg taaagtagcc atgtatatag
    23761 gaatgttatc aaaagaattt cctactaaat ttgtaaaagg tttaatcata tcaggagaaa
    23821 ttgatgattc attagttaat gccacacgta taacagacag gataaaagtt aaaacttatc
    23881 agatgaagtt atctttgcgt aatattattt gatggatgaa caccagtaag ctttcccggc
    23941 atttcaaata atctattcaa aaagttaaaa atggtgtata attaagtcaa ataatgttga
    24001 caggagggat attttggtaa agtggagtga tgactactta ataggagtac cagaaattga
    24061 cgaacaacat aaaagattat ttgaaatagc cggaagagct tataaagtca tgcaagatga
    24121 atacagcatt gataaatacg accaaattat tgaaatcatt gaagagttaa aagactatgc
    24181 tgtttatcat tttaagtctg aggaagaata tttaacaaaa atacaatata aaaaattctt
    24241 ttcacaaaaa attgctcatg atagttttgt cgaaaaaatt aataacatag attatgaaaa
    24301 aatagatgaa gaacaagata aatacttaat tgaaattatg gattttattg ttaattggat
    24361 tgttaatcat attattaaac tcgataaaga aattcctaat gtacccttat agatatcttt
    24421 tagattaata atttgttttg tattaaaata aatgattaaa gtcatggtaa aaataaacag
    24481 atacccttat taccatgact tttctgtatt tttattaatt ttaaggaggt gtctttatgt
    24541 ttttcgattt attaaagaaa aggagaagca ttagaaagta tataaataaa aaaattgaaa
    24601 aagaaaaagt ggatatgatt attaaaagtg ctttaatgtc tccttcatcc agaggaagga
    24661 ggccatggga gtttgttctt gtcgaagata gggatttgct ggaaaagctc tccaattgtc
    24721 gtgaacatgg ttcacagttc ctaaaagaag cagccctggc agtagtggtt ttagcagatc
    24781 ctgataaatg cgatgtatgg atagaagatg cttctattgc atcccttatc attcagttga
    24841 cagcccattc tttagattta ggttcttgtt ggatacaagt aagagaacgg tataaaaatg
    24901 aatttcaaaa aacagaagat tatattaaag aacttttaga tataccccct gattattgtg
    24961 ttgaatctat tattgccata ggttatcccg gagaagaaaa agaggctcat gatgaaaata
    25021 atttatccat ggataaggtt cattataata aatacaatat aaaatacagt taaaaaataa
    25081 ggaagttttt aacttcctta tttttataaa tggatttagc tcagataaaa caatacgtat
    25141 tttgagtccc tgatggcttt ttcgaagatc gttttaattt tacaaaaaac ataccaacta
    25201 cgttcaatga ttgtggattc taaggacttt tttaactctt caaaatctat ttcattatat
    25261 tctggaaatt cttcctcact atcgttatct gttgctgaag aatacagcac atcctttaga
    25321 atcttatccc agttatattt ttcgatccag tcttcctgac ggatatcttt aatgacttca
    25381 taagtttctt ttatttgcgt tggtgaaagg tatctggcgg ggccatatcc aacatcttca
    25441 tcgttaattt tttcccctcc taaaacgaca tacttgtatg gggcttgtcc ttcccattgt
    25501 tcaccacaca gggcataatg gatgatgtcc caggatgcac caatatttaa atagttttct
    25561 gagttttgat actcagggtc ttctaatatt gaagagatag agattttatt tgacaggata
    25621 tcctggtaaa tgttttcgtc aattaacaag taattaccgg ttttggacat ggcttatctc
    25681 ctttcgtata atattgtaat atttttttat atcataccac aatagtcgaa atatatctaa
    25741 catttactaa attttttgta aaaaaatctc ttaaaaatag ttgtttatcc ctttgttttt
    25801 ttgaataaat atagtatctt atttgtataa caatataaaa aagactggaa ggtgaagaca
    25861 atgaaggaaa aatacatttt attaaaaaac aatatgaaaa ttccccgttt aggtcagggg
    25921 acctggttta ttggagataa taaggcagaa gaaaaagatg aaatagaaag tatccgctta
    25981 gggattgaat tagggatgac acttattgat actgcagaaa tgtatggcag tggtgcggca
    26041 gaaagattaa ttggaaaagc cattaaaggt tatgatagaa gcaaactttt tattgtatca
    26101 aaagtttatc ctcataatgc aggtagaaga aatattttta atgcttgtga gaatagttta
    26161 aaaaggttgg atgtagatta tttggattta tatttgctcc attggagggg cagtattccc
    26221 ttaagagaaa ccgtggaatg catggaagag ttagtggcac aaggtaaaat cagaagttgg
    26281 ggtgtatcca attttgacag ctcagatatg aaagaattat ggtcagtacc caatggatct
    26341 aattgcgtta tcaatcaggt tttataccat ttaggatcaa ggggtatcga atatgattta
    26401 gtaccctgga tgaaagaaca taatgtgcct gtgatggcat attctccttt ggcacaggca
    26461 ggaagcttaa gacggggact gttagaaaat aaggttgttc gtgaaatagc acaaaacagg
    26521 ggaataagcc cagttcagct attattagct tttgtgttga gtcatgacaa tatgattgca
    26581 atccccaaat caggcaatcc aaagcatact cgggaaaatg cagaagtaat gaatatcatt
    26641 ttaacggaag aagaattaaa aatgttagac aaggcgtttc cggcacccaa ttataaaaca
    26701 ccattagata ttatctaata aggtttatta aattggaata atacattgaa aagttatcag
    26761 gaggtcctat gatttatttt aaagaagctg tcaataaaga agatattaga gaaatatcaa
    26821 aattagcaag aaagatattt gaagaatatt tcacacccat gattggcttt gaacaagtag
    26881 attatatgct taaaaaattt caaagcgaag aagccctgag caagcaaatc agggatggct
    26941 gtaagtattt tatggtatta gaagataata aattagcagg atattttgct ataaagccag
    27001 aagaaaaccg gttatttctg agtaaatttt atttagataa agaatacaga ggtttaggga
    27061 tagccagaaa aatgatggaa gaaatcatca aattttccca atctcaggct ttaccagcca
    27121 tatatcttac agtcaataag aaaaatgagc gcacgataaa tatttataaa aagtttggtt
    27181 ttgagattat aaagagtgtg gaaaccgata taggtcaggg tttttttatg gatgattata
    27241 tcatggaaaa gaagttatca taataagatt cataggattt aaacaaaaat agttaaagtt
    27301 attccggatt atagtttaaa atataaagga tagtaaaggg tgaaaattac atttatttta
    27361 gaatcagaca aggatttatt gacataatag taataaaatt ttataattaa ttttataatg
    27421 tcaatgaatt tttgaggaga atgggtggga aatatgacaa aaaaatttat tattattttt
    27481 gttttgtttt ttatgatggc gagcagcctt ttttgttata aaaataatag aacaaatact
    27541 tctcagacat tagaactaag tgacagagtc gaagccagtg agattaaaga agagcctata
    27601 gtgaaggaac tggcttttta cattggaagc cacaaatggt atattgacag gcaagaacaa
    27661 aatgatatgg aagagtataa aaaaataaaa ccaattataa gggataacac aattttattt
    27721 cctataggtg tggtattaag aaaatttagt gaggatgttt catgggatcc agtgggaaaa
    27781 acgatatctg caacgcttaa tggagaaagt tttaaccttt caattaatga aaaaaaggtt
    27841 tctgttggag atgaattttt taatttaaaa gatgatatta aagtttatgg aaatcaatat
    27901 tatctttctg cagaagatac agccaggata ttaaatttaa gatttcaggt aaagaatggg
    27961 gtaattgctt ttgtacacca ggataatcat ttagatgaag ccataaatta ttatacggat
    28021 aagtataacg aggaattatc gaatataaaa gataggacat caataaaatc cttaaaaatt
    28081 cctgtactga tgtatcactt atgtttaacg gaagcgcctc cggatgaact taaagggatg
    28141 gcggttactg ttaaagaatt tgatgctcag ttagcagcat taaaaaaagc aggttatgaa
    28201 ggtatatcct ttgaacaata ttataattat aggctttatg gggcatcatt gccagaaaaa
    28261 ccagttatca taacttttga tgatggctat gaaaataatt atacaaatgc atttccaatt
    28321 cttaagaaac ataatatgaa agctactatt tttatagtaa cttctgcagt aggacagcaa
    28381 ggggttcaat atccccatat gacatgggaa cagatgaaag aaatggaaga cagcggtctt
    28441 gtgataattc acaatcatgg ttcctaccac gccgccatgg atcaaatatc aagatcaaat
    28501 atgagaaaag tcgcattagg tggatatacc tctatagagc atcatatggg aacaagacaa
    28561 atttatgttt ttgcttatcc ttatttaaga tacaatgcgg taactataga ggaattaaaa
    28621 caagcaggta tacatatgca agtgactaat ttaggggatt ggttaagaga aggaacttct
    28681 atgtccgata taaaaagaat aaggatttca catgggacat cggagcagca attgttgaat
    28741 agtataaaat aattttagat cgtcagttct ttttaataaa ggttgagtta tgcattattt
    28801 taataagagg aaaaattgaa aaagcatggt ttaagtcatt gataaaagga ttataagtaa
    28861 attaatacca atataaagat ttatttttta agggagctgt aatgttttta tatacggctc
    28921 ccttttaggt tataacaaaa ggcggttatg atgcttcttt ttaattttct tgttttttct
    28981 tgtaatttac gataaggatt gaagtgatta ttttcccaat tatggatatg attaaaacag
    29041 agaatctgaa gattgtttgt gcctgagttc ttgcaggatt ttttgataat atcagcatca
    29101 taataaaaga aaatataaag aagtagtcta atgctgctaa tggagcaggc acttttttac
    29161 ttgtaatagt ttgttttgtc tgataaccaa aggcattatt tttatccttt ttactcaata
    29221 agtttttata atagctaata aggaatttaa aaacacctga ccaggtgtat tcataatggt
    29281 tttcatcagg cttatatttt agtattttca ggtctgccat tttgtctaag attctcttat
    29341 tctcttcgtt cattatattt ggatagtctt ttgacaggat agtattatcc aggcttttag
    29401 aatgtataag ttcattaatc ttattaaaat ggttatgaag taaatcatca aaatcagcca
    29461 tgtctttata agtcaataca atattattgt tatcaaagtc atcataatca aaaaaggatt
    29521 tttcaggttc aatcctcgaa accagatagc ttccgtcatg aaatcttgta attaattcaa
    29581 aagtatattt ttggacctga gtttttattt cttcattttc aatttgttga attttatacg
    29641 aataggagta aaggatttta gctaaagtac agaattcttt atggataaat attttggaat
    29701 ataaattaac atctaattgt tttgtagtca ttttatagct gttttccaag ataaaacctt
    29761 gattataaag cttattttct aaatcattaa ttaagttgag gtttatgttt ggaattgagt
    29821 taaagtcatg gggtttaacg ataagtgtat cataaatctt ttcttttttc atcatgataa
    29881 tgatacaaat atttacaatg aaaactactg ccagaacaat aattaaaagg attaagacaa
    29941 tttttcctat taaatgaatt accattacta tggcacctcc taaacaaatg tttt
//
LOCUS       DAOSUD010000019        29747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1053_length_29747_cov_23.440388, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000019 DAOSUD010000000
VERSION     DAOSUD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 29747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29747
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1053_length_29747_cov_23.440388"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            879..1271
                     /locus_tag="PLG49_04090"
     CDS             879..1271
                     /locus_tag="PLG49_04090"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HOQ16532.1"
                     /translation="MRIKVEINKKYLFCFIILFLIESLIAIFIHDNFIRPYLGDLLVI
                     ILIYCFISIFLKKKIKLLLLYIFIFAVIVEGLQYIHILERLKLNHIPILKIVLGSTFD
                     IKDILCYFIGMVILFLWQKRTSHESINE"
     gene            complement(1342..3750)
                     /locus_tag="PLG49_04095"
     CDS             complement(1342..3750)
                     /locus_tag="PLG49_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HOQ16533.1"
                     /translation="MRYGYFDEKNKEYVITKPNTPAPWVNYLGSPEYGAIISNNAGGY
                     SFVKSGANGRIIRYRFNAINMDQPGRYIYIRDKETSDYWTTSWQPVGKDLEKYESECR
                     HGTAYTTMLARYDNIESNVLYYVPLNKTYEVWRLKIKNNDSKARKLSVFGFVEFTNDD
                     NYEQDSVNLQYTQFITRTEFKDNKIVQYINEYVRKSKAERDGNTGSERFFGAVGLPVI
                     AYCGDRDVFVGDYRNYSNPIEVVNGKLSNTLNYNGNSCGALQIDLDLEPGESKELAFI
                     LGAYGDEEATKIMNAYNNPELIDKELEELKNYWHSKLNSLQVKTPDENFNHMVNTWNA
                     FQCFITFIWSRAASFTYCGLRNGYGYRDTVQDIQGIIHLDPEMALDKIRFMLSAQVDN
                     GGGLPLVKFNHNAGHENTPDDPEYVKETGHPHYRADDALWLFPTILKYINESGNIDFI
                     DEVIVFANGGEGTVYEHLKRAINFSMERLGAHGMPAGLHADWNDCLRMGAKGESTFVA
                     FQLYYAIKVLRDFAELKEDKEYIAYLDEIKDKLSDAIQKYCWDEDRFIRGIREDGVVV
                     GSKKDKEASMWLNPQSWSVISGFASEEQAKLAMDNVYNILNTEYGAMLMYPPYKEHAF
                     DGALMLLFNACAKENAGIFSQPQGWLILAETLLGHGNRAYEYYKKCNPAQMNDKAEIR
                     KIEPYAHGQFIEGKDSPYHGRAHVHWLTGTASTVMVATVEGILGLKPNIKGIVIDPCI
                     PSDWTELTIRKKFRGKWLSITVKNQSKTEKGVKETYLNGVKLEDNFIAEEALKDNNDV
                     LIIM"
     gene            complement(3830..4300)
                     /gene="nrdR"
                     /locus_tag="PLG49_04100"
     CDS             complement(3830..4300)
                     /gene="nrdR"
                     /locus_tag="PLG49_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOQ16534.1"
                     /translation="MRCPFCNGANTRVIDSRPSEENNSIRRRRQCENCERRFTTYERV
                     ETIPMMVIKRDGTRELFDRNKILNGIVKSCNKRNISLKQMEAIVDDIENIIFNSLQTE
                     VKSTQIGELVMEKLKEIDEVAYVRFASVYRQFKDINTFMDELKKLLNENKNNDI"
     gene            complement(4408..4671)
                     /locus_tag="PLG49_04105"
     CDS             complement(4408..4671)
                     /locus_tag="PLG49_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOQ16535.1"
                     /translation="MVRIYDMKQKEVISTIDGCRLGYICDIEIDLIEGRIKKIIVPGH
                     GKLLGFFGKEMEYQIPWHKIRKIGEDIILVDVTGKDVLVESEY"
     gene            complement(4810..5586)
                     /gene="sigG"
                     /locus_tag="PLG49_04110"
     CDS             complement(4810..5586)
                     /gene="sigG"
                     /locus_tag="PLG49_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOQ16536.1"
                     /translation="MAINKVEICGVNTSKLPVLTNEEKEELFKRILQGDKEARESYIK
                     GNLRLVLSVIQRFNNRGEQMDDLFQVGCIGLIKAIDNFDINQNVKFSTYAVPMIIGEI
                     RRYLRDNNSIRVSRSLRDTAYKALQVKEKLINKNSKEPTITEIAEELGMPKEEVVFAL
                     DAIQDPISLFEPVYHESGGDALSIMDQVSDEKNQDEIWLENIALNEAMQKLNEREKHI
                     LSLRFFEGKTQMEVAEEIGISQAQVSRLEKTALKHMRKYI"
     gene            complement(5658..6389)
                     /gene="sigE"
                     /locus_tag="PLG49_04115"
     CDS             complement(5658..6389)
                     /gene="sigE"
                     /locus_tag="PLG49_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOQ16537.1"
                     /translation="MSFTDKISLKFKYFIYRLLNFFGFKESIFYIGGSEILPPPLNRE
                     EEESLIKRLGTKDDLYVKSVLIERNLRLVVYIARKFENTGIGVEDLISIGTIGLIKSI
                     NTFKPDKRIKLATYASRCIENEILMYLRRTNKLKTEISIDEPLNVDWDGNELLLSDIL
                     GTDPDIIYRSIEDEIDRDLLRKAMEKLSSREKEIVELRFGLNTEGKEKTQKEVADMLG
                     ISQSYISRLEKKIISRLKKEINRMN"
     gene            complement(6417..7346)
                     /gene="spoIIGA"
                     /locus_tag="PLG49_04120"
     CDS             complement(6417..7346)
                     /gene="spoIIGA"
                     /locus_tag="PLG49_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOQ16538.1"
                     /translation="MNQGFAIEIYIDMVFLLNFVMDYFILWIVSKIIKEKVNHKRLIF
                     GALLAALIYCLILFVPIFNQSYNFFGILILPMFPLMITYKTKTFKKLMKVFILFHITA
                     FVLGGAGIAFFYYLNVGQMIKNSLQFNIDNFPIILLIVSSLLSYLVIKLLLIWIRIFS
                     NHTGALYQIKISYEGMKIDACALMDTGNALYDPFSQSPVIVVEFSSIKKFFPESIQEL
                     FNEKKENDLGLLTKYISGCPIRSKIRVIPFSSLGTPNGMLLGFKPDQVEIIEKDHSIK
                     VLKDVVIGIYNQRLSKDNKYQALLHPDIFHEIA"
     gene            complement(7567..8667)
                     /gene="ftsZ"
                     /locus_tag="PLG49_04125"
     CDS             complement(7567..8667)
                     /gene="ftsZ"
                     /locus_tag="PLG49_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOQ16539.1"
                     /translation="MLEFDMTQDHVAQIKVVGVGGGGNNAVDRMIEENLKGVEFITIN
                     TDKQALGRSKASVKIQIGEKITRGLGAGANPEIGCKSAEESRDDILEALKGADMVFVT
                     AGMGGGTGTGAAPVVARIAKEEGILTVGVVTKPFSFEGRKRMLNAEKGIEELKKNVDT
                     LVIIPNDKLLHIIDKKTSIIEAFKKADEVLRQGVQGIADLISNPGIINLDFADVRTIM
                     SDKGIAHMGIGQASGENKTEIAAKAAIHSPLLDTSIDGAKGVLINISGGSNLGLLEAN
                     EAANLIREAVDPEAEIIFGTTINDDLGDSVIVTVIATGFENRIAKPSIKVPKVESIAV
                     SLKEEKSSTVINNVPDDSSFIDIPVFLQRRKK"
     gene            complement(8832..9578)
                     /locus_tag="PLG49_04130"
     CDS             complement(8832..9578)
                     /locus_tag="PLG49_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOQ16540.1"
                     /translation="MGKVVKMGNFQSTHQHKTVKILLFIGMLVVLFLLLFSSPLFSIH
                     HINIYGNDHYSKEEIIAMIGVNKGDNIFLFNKKAGEKILNEDVYIESASIRIIWPDTV
                     EIKVDERKLVGYVPYLGTYLYIDKDGRVLESCKTYDKNLPIVEGLEFDYFQVGEIIPA
                     KNKNALDVVAIMSQMMLKYDMLQDVVKVSVSDPENIHIYVRNLDVSIGSVEDFDKKIQ
                     WLVQIMDIYDMGKLDLRNVDKGKAIFSPLT"
     gene            complement(9600..10850)
                     /gene="murA"
                     /locus_tag="PLG49_04135"
     CDS             complement(9600..10850)
                     /gene="murA"
                     /locus_tag="PLG49_04135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ16541.1"
                     /translation="MGRFYIDGGRCLEGTLRIQGGKNAVLPILAATVLNGDVSYIQDC
                     PRILDVFTMIRILESIGCKVRWDDKTLMIDSSSVDSYKIPEELVREMRSSIILLGSVL
                     GRHKKVIISYPGGCPLGPRPIDLHIKALKQLGASIKEEHGFIICEAPKLVGAKIHLDF
                     PSVGATENIMLAAVLAEGTTEIHNAAKEPEIKDLQDFLNGMGAKIKGASSDVITIEGV
                     KSLHQVEHKVIPDRIVTGTYLAAAAITGGEVLLTNVNPNHIQAINSKLKEAGCRIIEE
                     KDRIYLKAPKKLKNIDIIRTQPYPGFPTDMQAQMMAMLTVASGASIITETVFESRYKH
                     VDELIRMGANITLEGRTAIIKGVGHLTGTNVYAKDLRGGAALIIAGLVAEGVTIVDGA
                     KHIERGYEQIDKDLNILGASIRKD"
     gene            complement(11020..12183)
                     /gene="ftsW"
                     /locus_tag="PLG49_04140"
     CDS             complement(11020..12183)
                     /gene="ftsW"
                     /locus_tag="PLG49_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOQ16542.1"
                     /translation="MDLAVSQKGYKKAKRKKEEVKLVRGQCDFTILFTVVTLTLFGVV
                     MIFSSSYHYALENFNDMYYFLKRQVIWSLLGFATMIFAMNFDYRRLKVWAPLLYLISQ
                     VLLVLVLFIGKEVNNSKRWFGIGSIGFQPSELAKLTLIFFLALYISRNKSKLKTFTGL
                     FLTLIIVVGIPAGLIAVANLSTAIVVAFIGIAMLFVASPKVWHFFALAAPVAAAAIPA
                     VLLPQFRYRLVRIQIWLNPWLDPTNKGYQPIQSLYAVGSGGLFGLGLGESRQKLGFIP
                     EAHNDIIFAIVCEELGLVGAAVLLTLFIVLIWRGIKTAMNAPDLFGSLIATGIVCMIG
                     IQVLINVAVITNTIPTTGMPLPFISYGGSSLLFTMAAIGILLNISRYSRTNKI"
     gene            complement(12188..13558)
                     /gene="murD"
                     /locus_tag="PLG49_04145"
     CDS             complement(12188..13558)
                     /gene="murD"
                     /locus_tag="PLG49_04145"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOQ16543.1"
                     /translation="MDWCKKNVLVVGMAKSGISAAKLCSRLGAKVTIQDVKSKEELMK
                     SVKEEIDILDSMAVYMLLGEEPTDIIDSFDLIVLSPGVPTDLEFLNKARQLQIPIWSE
                     IELAFVLCPCPVIAITGTNGKTTTTTLVGEIMKKYNPGTQVVGNIGIPFTEKVEELKK
                     EDLVVAEISSFQLESIHTFKPKVSAILNITPDHLNRHKSLENYVAAKERIFENQDEND
                     YCVLNDDDDICSKMKHKTKAKALLFSRKKELNEGAFLKDNKIWVRLGKEDEAICLTEE
                     LRIPGNHNIENVLAAVAISVSMGVPPEIIRDVVIHFKGVEHRIEFVDKIDGVEYYNDS
                     KATNVDAAIIGIQSMKAPVVLIGGGMDKGADFSEWIDAFDGKVKALIVMGETADKIIK
                     TANQKGFFNIKKVSSIEEAVRLAKETAQKGDCVLLSPACASWDMFKSYEERGKLFKKA
                     VYALRG"
     gene            complement(13588..14544)
                     /gene="mraY"
                     /locus_tag="PLG49_04150"
     CDS             complement(13588..14544)
                     /gene="mraY"
                     /locus_tag="PLG49_04150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOQ16544.1"
                     /translation="MYHEAIYAVLISFIINLILSPILIPFLIKLKFGQFVRDDGPKSH
                     LKKAGTPTMGGIIILSSILLTSLLFIKGNRDGLAVLLVTLSFGIIGFIDDFIKVALKR
                     SLGLKAYQKIVGQLFVSGCFLYYILNVAKIGTRVIIPFTAGREIDFGILFIPFFFIVM
                     LGTVNGVNLTDGLDGLVSSVTVLIATFFTVVSWGENSSILPVAAAVVGSLLSFLLFNS
                     YPAKVFMGDTGSLALGGFVVSTAFILKMPIFIAIVGMIYLIESISVMIQVAYFKATGK
                     RVFKMAPIHHHFELSGWTESKVVSVFCIATAVACLIGFLGLN"
     gene            complement(14548..15948)
                     /gene="murF"
                     /locus_tag="PLG49_04155"
     CDS             complement(14548..15948)
                     /gene="murF"
                     /locus_tag="PLG49_04155"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOQ16545.1"
                     /translation="MEAIKISEIVSAVKGIFINPSKNIDEHQIKIDSLSTDSRVISAG
                     DLFIPLTGDKFDGHDFIPAAFEKGALLSLSQRRDIKIPEGKGLILVEDTKQALMDLAT
                     YYRSLFSIPVIAVTGSVGKTSTKDMIASVLGQSFKVLKTQGNFNNEIGVPLTIFNLSR
                     EDEIAVIEMGMNHFGEIHNLSKIARPDMAVITNIGTSHIENLGSREGILKAKSEIFDY
                     MKKKGKAFLNGDDDLLVSLRDQLTVPVCFFGLSHSNDIYANKIEEKGLEGTYASIVWD
                     SNEMQIEIPVPGRHMVYNALVAVAIGLELGMTKEKIKEGIKNYQASKMRMNISRNKKG
                     IYIINDAYNASPQSMKAALDVLSQLKEGKRKIAVLGDMLELGTYSEQAHKEVGEYVAK
                     KALDYLFTFGREAENIALAAMNAGMDKNKVFIFLDQEELFNQLQMLVDSNDMILFKAS
                     RGMHLEKTVEKLEEVE"
     gene            complement(15929..17398)
                     /locus_tag="PLG49_04160"
     CDS             complement(15929..17398)
                     /locus_tag="PLG49_04160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOQ16546.1"
                     /translation="MKVKELLKEIDYRLLQGDLDKEVESIAYDSRQVKEKGLFVCIEG
                     YVSDGHKFIPDAIAKGAGSLLVSKKVENLPSDITVIQVEDTRKALASVAAAFYGYPAN
                     QIKLIGVTGTNGKTTTAYLIGRILEEYNNKVAVLGTIENRIGNKVLKTERTTPESLDL
                     QALFSEMLKEEVSHCVMEVSSHALELSRVAGCYFEIGIFTNLSLDHLDFHKTMENYKM
                     AKAKLFKNCKYGIINMDDASASDIIKASSCEIITFAIEKEADFRAYDIHISSKGIEFT
                     VKIDHEEVRFKLNTIGRFNVYNALGAIVAAYYLNIPKEVIKTALEKMEGVPGRVQNVS
                     SHSDFNILVDYAHTPDGLENVLKTIKEFAKGEIITVFGCGGDRDKSKRPIMGKIAGEY
                     SSYCIITSDNPRTEDPEKIIDEIEAGIKESNCPYEKIVDRREAIKKAVQRAKKDDVIL
                     IAGKGHENYQILKDKVVHFDDAEEVRAILQEDYNGSHKN"
     gene            complement(17493..19442)
                     /locus_tag="PLG49_04165"
     CDS             complement(17493..19442)
                     /locus_tag="PLG49_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ16547.1"
                     /translation="MDKPTIKMKRKLLFFLCAFTFSIIGLIIRIAYLQIAHGSFLQEL
                     AYEQHTRDRLISPQRGSIYDRNGVELAKSATVATIGVVHAQVEDSEEVTRVLSEKLGL
                     DYEYVKKKVEKNVALERIQSKVDKELADEIRKLNLAGVKIDEDSKRYYPYGNLASHVI
                     GFVGKDNQGIIGLEVKYDEYLKGSPGKILMETDGRGREIKDIPERRIEPVPGYHLVTT
                     LDVNIQQYAEQALEKAVKAKGAKRGSIIIMNPQTGAIYAMANKPDYDLNQPFKINNPD
                     LEAIWDSLTPEEQHKALNQMWRNFAINDTYEPGSTFKIVTSAIGLEEKIVNIDSPFYC
                     KGYHLVGDRMIKCWRYPRAHGSETFTQGVQNSCNPVFMEVAERVGPKKYYQYMKKFGF
                     HEKTGIDVPGEAVGIMHDLKDIGPVELATMSFGQSFQITPLQLIRAASAAVNGGYLVT
                     PHFASKIIDDEGNVIKTFEYEKGKQVISEETSQVLKKILESVVAEGTGNKAYIPGYRI
                     GGKTATSEKLPRSSKKYIASFLAFAPAEDPQVIALIIIDEPQGIYYGGTVAGPVIQEI
                     FENILPYLNIKPQYTEKEMKMNLVEKIVVPDLISKNVQDAKNELKKLNLGVELIGNGE
                     IVHDQFPLAGDEVNPNSKVILYTDP"
     gene            complement(19550..21550)
                     /locus_tag="PLG49_04170"
     CDS             complement(19550..21550)
                     /locus_tag="PLG49_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ16548.1"
                     /translation="MNELIRQVRRRNFIILTILIGSILLLIYRIGYIKYTYGEEYEER
                     AVLQRLNTEQVIYPNRGFIVDRNFHDLAISTIVYNVILDPNILAEHITEEKRNEVVAD
                     LAQILNLSPESIQQTIEKNPKSRYQIIKKHVKADTAQKIREKGLMGVWLEEDSFRNYV
                     RNDFAAHLLGFVNADKQAQYGVEQYYDNWLRGVSGRVFPMIKEGKYVMEENIAPQQGH
                     TVVLTIDETIQHFCELALKNAVEEHNPKNAAVIAMNPKTGEILAMAAYPSFNPNKYND
                     LSEYFDEGIWESLSNEEKLKMLNTVWKNYNLSVTYEPGSTFKPFVIAAALEENSITVH
                     DKYNCLGHKKIHTETIHCWRTWGHGEETLEQALANSCNVAMMDIVEKLGAEKFYRYQK
                     LFGFGEVTGIDLPGEESGILHKQEALRPVELATSSFGQTFNVTPIQLITGFSAIINGG
                     NLMQPYVVKQIVTEDGREVKTTSPVIKRRVISQETSQILRNYLETVVTDGTGKNARVE
                     GYTVGGKTGTAEKLPRGSGKYVLSFIGFAPVEDPQIVVLAILDETENYSEGSVSAAPI
                     VREIFENVLPYMGIEPSGQTNIENKTDVELPDYSGLTLLETGTDLTVKGLKFEAIGMG
                     NYVKKQFPNPGTRVPLGTTIKLYVTENPEEIETTEEESVTEE"
     gene            complement(21555..22091)
                     /locus_tag="PLG49_04175"
     CDS             complement(21555..22091)
                     /locus_tag="PLG49_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ16549.1"
                     /translation="MSKSNRIREQYLYGTSAPKFNDTHKTLIQEDEKELLHYKQKKEK
                     AQRTRKVISYKLKLVGNILCLFIGCLFIMGQYASITYNQKEIKKLKSELKEIQNSNML
                     LKSEIAETVDLTYIKEVATEKLGMMEPAAHQIVYIEIPKVSYTAYNQLEQDQNRLNQS
                     TEDEVVAASFFDFFKLKR"
     gene            complement(22163..23098)
                     /gene="rsmH"
                     /locus_tag="PLG49_04180"
     CDS             complement(22163..23098)
                     /gene="rsmH"
                     /locus_tag="PLG49_04180"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ16550.1"
                     /translation="MDFHHISVLLEETIEGLNIDPSGIYVDGTMGGAGHSYEICKHLN
                     EYGRFIGIDRDIDAIKASEIKLKSQFKNVDLVHDNFSNIKKILIDLGLSSINGMLLDL
                     GVSSYQLDTPDRGFSYMHNAPLDMRMDRNQALTAREIVNEYSQKRLEEIIKEYGEERW
                     AKRIAEFIVNERKKAPISTTFELVDIIKKAIPQGARKEGPHPAKRTFQAIRIEVNGEL
                     KILEQSIKDIVDVLAPKGRLCIITFHSLEDRIVKKSFKALENPCTCPPDFPICVCGKK
                     AMARVISRKPILPSPQEIEKNPRSRSAKLRILEKL"
     gene            complement(23115..23543)
                     /gene="mraZ"
                     /locus_tag="PLG49_04185"
     CDS             complement(23115..23543)
                     /gene="mraZ"
                     /locus_tag="PLG49_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOQ16551.1"
                     /translation="MFMGEYQHSIDNKGRLIIPAKFREELGDTFVITKGLDNCLFVYP
                     NSEWKIFEEKLKTLPITNANARKFVRFFLAGAVECNVDKQGRILIPNNLRNYSGIDKD
                     VILIGVTNRVEIWSKENWDKYNNEDFDASELAENMQELGI"
     gene            complement(23705..23872)
                     /locus_tag="PLG49_04190"
     CDS             complement(23705..23872)
                     /locus_tag="PLG49_04190"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HOQ16552.1"
                     /translation="MNNIYGEINLLREKLNELIVGENDFQEIYRISTELDRLILLYYD
                     HISCNTRSLDA"
     gene            complement(24006..24866)
                     /gene="lgt"
                     /locus_tag="PLG49_04195"
     CDS             complement(24006..24866)
                     /gene="lgt"
                     /locus_tag="PLG49_04195"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ16553.1"
                     /translation="MSAPEVWFPHIGIKIGKLDPVAFKLFGISVYWYGIIIGLAVTCG
                     LLLAMEEAKRTNQDPNYYVDFLLYDLIAAIIGARLYYVIFSWEDYKDNWLRIFALREG
                     GLAIYGGIITSVIVAWIFTKKKNISFGLFADTAAPSLILGQAIGRWGNFFNQEAFGRY
                     TDSLFAMRLIKENVNQYLTPDILNNTILFEGIEYIQVHPTFLYECLWNLLVLFFLLWY
                     KKRKKFNGEVFCMYLIGYGLGRSWIEGLRIDQLQIGSTGLAVSQLLSILLVVCSIFLI
                     INNRKKYKRN"
     gene            complement(25030..25176)
                     /locus_tag="PLG49_04200"
     CDS             complement(25030..25176)
                     /locus_tag="PLG49_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16554.1"
                     /translation="MKYFFNLLLMPKGRLIIFRKLFAVIIVIALIVLIVVLHKIKAEW
                     VAIN"
     gene            complement(25318..25830)
                     /locus_tag="PLG49_04205"
     CDS             complement(25318..25830)
                     /locus_tag="PLG49_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HOQ16555.1"
                     /translation="MRNNISGYYIENSIERLNRRDNTAEDIITAKGSIQEFNRSYPYN
                     IPDDVERINNHYFDIGYNKERANRIAKLINDFPEVNKTTVIITGNTALIGIDTVHELD
                     KKEIERLKRKIEEKVYSTDLSLKNIGISTSPEIINRMNKITNSINNMKSIDGLANELS
                     SIIREIVPNV"
     gene            26159..26341
                     /locus_tag="PLG49_04210"
     CDS             26159..26341
                     /locus_tag="PLG49_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16556.1"
                     /translation="MNARTQQRHYRVAQKEIEHAPNKGIFIENDKKQLYAIIAGVILV
                     LTFIQGLIIGLFISRD"
     gene            26509..27606
                     /gene="ychF"
                     /locus_tag="PLG49_04215"
     CDS             26509..27606
                     /gene="ychF"
                     /locus_tag="PLG49_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOQ16557.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKGGAESANYPFCTIDPNIGIVAV
                     PDERLQALDKIYNAEKVTPAIIEFVDIAGLVKGASHGEGLGNKFLSHIREVDAIVHVV
                     RCFEDTNIVHVEGSIDPIRDIETINLELIFSDIEILDRRIQKTEKAAKADKGLLKELE
                     ILEKIKKALEDGKCARNVPLESDEEKEFVSSLNLLTYKPVLYAANVSEEDLISNAEDN
                     SYVKKVKEYAKAENSEVFVICAKIEEEIAQLDEEEKKLFLEDLGISDTGLDRLIAASY
                     KLLGLISFLTAGPKEVRAWTIVKGTKAPQAAGKIHSDFERGFIRAEVISFEDLIACGN
                     YTIAKEKGLVRLEGKDYVVQDGDVIIFRFNV"
     gene            complement(27651..28055)
                     /locus_tag="PLG49_04220"
     CDS             complement(27651..28055)
                     /locus_tag="PLG49_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOQ16558.1"
                     /translation="MESNLKTNLESLFSQLESFISTKTVVGEPIKVEDAIILPLVDVC
                     FGVGAGASGGKTDKGEDKDSGMGGLGAKITPSAILLIKDGTTQLINIKNQDSVNKLID
                     MIPELLSKFNIGKKKQCKTDAQEKEERTEGEI"
     gene            complement(28069..29001)
                     /locus_tag="PLG49_04225"
     CDS             complement(28069..29001)
                     /locus_tag="PLG49_04225"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOQ16559.1"
                     /translation="MSIISVLLLVLKYIGIILATLLGILILILLLLLFIPFRYFVYLS
                     ADGNFAIDVNVKIRWIFNWIYYCFVMKKSKKETKIFRVFSKKIINEQRRVKKESMQKD
                     KIKKQGKTSGAIENIQLESKEEKKDDRIKNEEIETKYKIKEKSINDFSKENDVSKKVV
                     KSHKNSLESEDVNKYNDIDEIVETDVIEEAEDAGFSLRNLLDYPNKKEIIMYTYQFLK
                     KIFIHIRPRTFSSYLEFGLDDPSHTGYLLGAIGIISPFLGNSVHIKANFNEKILQGEL
                     EARGSFQIGIIIIYLANYLLKKPIREIVKNYLSS"
     gene            complement(29014..29325)
                     /locus_tag="PLG49_04230"
     CDS             complement(29014..29325)
                     /locus_tag="PLG49_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16560.1"
                     /translation="MKVAKNYYTGDSIKNKERIVKRLIKNYLYKDIYCLCLEPSSEYL
                     MVIIKTNEMKNNISRYRDCKVVGIARNKEEAFELTRRIIDDVYKKYKDPMMIKEFFAW
                     E"
     gene            complement(29436..29606)
                     /locus_tag="PLG49_04235"
     CDS             complement(29436..29606)
                     /locus_tag="PLG49_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16561.1"
                     /translation="MEQTQFEQLKKKFQSSTVDEMIDIYISTEGLTQEQYMELLRVFP
                     REHISKLEAALQ"
BASE COUNT         9271 a         6004 c         3929 g        10543 t
ORIGIN      
        1 ttcttgtttt tcttctcctc ctgttgatgg agtttctaca gcagtttcac cgcctgtagt
       61 tccttgacct ttgtctttgt tacatcctgt gaacatcaca agaaccatgg tcaagcagat
      121 aagtaaagcc acgcttcttt ttaaaagctt catctaaaat acccctctct tttttgttaa
      181 aattatataa aaggtataaa taccttttat aactccccta agtaacaaag cttttttttt
      241 ttttaaatag tgcagattgc ttaattaccc tgaaaaataa gtaaatgtgc atctgtgaat
      301 gcgcgtttac aattcaatgt tactactttt tcacaaaaaa ttcaagggtt tatgttaaaa
      361 taatataaaa atttttaaat aattaatatc attatttata tatgaatagt tataattgtg
      421 tattttattt aatttttaat ataaaaatta acgtttttat taaaatatat tttctgtatg
      481 aacgcgtgat tattttaagt atatcactct tacaccatta aatcaatata attctaaatt
      541 tataaactta cctctagtat atttttttac agatatctta atcattatca aaaggaagtc
      601 tcaagttgaa agtctcttaa tccaacttga ggctatcatt tattactata tcagaccctt
      661 tagacatctt aatctcatcc aatagtaatt ctatctcctt ccaattctgt acatacaaaa
      721 agattcctat tcaaaatcaa tgccgccctt gctccactcc attacgtcaa tctgtctgag
      781 agttattatt aaaattccat tttactaccc attatgatca tcttttgata agatagttta
      841 taacatattc tttatcttat aaataagaag tttttataat gagaattaaa gtggaaataa
      901 ataaaaaata cctgttctgc tttattatcc tttttctcat tgagagtctt attgcaatct
      961 ttatccatga taattttatc cgtccatatt taggagatct tttagttatt atcctaatct
     1021 attgctttat ttctatattt ttaaagaaaa aaataaaatt attgctctta tatattttta
     1081 tttttgctgt tatcgtagaa ggactgcaat atatccatat actagaacgg ctcaaattaa
     1141 atcatattcc aatattaaaa attgtcctgg gcagtacctt tgatataaag gatattttat
     1201 gttatttcat tggtatggtt attctatttt tatggcaaaa aagaacttca cacgaatcaa
     1261 taaatgaata acgcttatta tacaaaagca aagggctgtc gcattaagga tatttatccg
     1321 ttcatgcgac agccctgttt cttacataat gattaaaacg tcattattat cttttaaagc
     1381 ttcttcagcg ataaaattgt cttccagctt aacgccattt aaataagttt cttttacacc
     1441 tttctcagtt ttactttgat tttttactgt gatgcttaac catttccccc tgaatttttt
     1501 acgaattgta agttctgtcc aatcagaagg aatgcatgga tcaatgacaa ttcctttaat
     1561 attgggttta aggcccagta taccttcaac agtagccacc ataactgttg aagctgttcc
     1621 ggttagccag tgcacatgag cacgtccatg ataaggacta tcttttcctt caataaattg
     1681 cccatgagca taaggctcaa tttttcttat ttctgcctta tcattcattt gggcaggatt
     1741 acatttttta taatactcat atgccctgtt tccatgacct aataaggttt ctgctaaaat
     1801 aagccagccc tggggttgtg aaaagatacc tgcattttct ttagcgcaag cattaaataa
     1861 aagcatcagt gccccatcaa aagcatgttc tttataaggc ggatacatca gcatagcacc
     1921 atattccgta tttaaaatat tataaacgtt atccatggcc agtttagctt gttcttcact
     1981 ggcaaaacca cttattacag accaggattg cgggttaagc cacatacttg cttctttatc
     2041 tttcttagag cctacaacaa cgccgtcttc cctgatacct cttataaatc tgtcttcatc
     2101 ccagcaatat ttttgaatag catcagacaa tttatcctta atttcatcca aataagcgat
     2161 atattcctta tcctctttta attctgcaaa atctcttaag actttaatcg cataatataa
     2221 ttggaaagca acaaaagtgg attcaccctt agcccccatt cttaaacagt cattccagtc
     2281 tgcatgaaga cctgcaggca ttccatgagc tcctaatctt tccatgctaa aattaatagc
     2341 tcttttcaaa tgctcataaa cggtaccttc gccaccatta gcaaagacaa tcacttcatc
     2401 aataaaatcg atattgccgc tttcattaat gtattttaat atggttggga ataaccataa
     2461 agcatcatcg gccctgtaat gcggatggcc tgtttctttc acatattcag gatcatccgg
     2521 tgtattttca tgacctgcat tatggttaaa cttgactaaa ggcagtcctc ccccattatc
     2581 aacctgggca gaaagcataa aacgtatctt atccagagcc atctccggat ctaaatgtat
     2641 aataccttga atatcctgca ctgtatccct gtaaccatat ccgtttctta aaccgcagta
     2701 agtaaaagac gctgctctgg accaaataaa ggtaataaaa cattggaagg cattccatgt
     2761 attaaccata tgattaaaat tctcatctgg cgtttttact tgtaaactat ttaatttact
     2821 gtgccaataa tttttcaatt cttctaattc tttatcaata agttcgggat tattataggc
     2881 attcattatt ttagtagctt cttcatcacc ataagcaccc agaataaaag caagttcttt
     2941 actttcacca ggctctaaat ccaaatcaat ttgcaatgcc ccacaggaat taccattata
     3001 attcagggta ttgctcaatt ttccattaac cacttcaata ggattggaat aatttctata
     3061 atcacctaca aaaacatctc tgtcaccaca ataagcaatc accggcaaac caacggcacc
     3121 aaaaaacctt tcactgccag tattgccatc ccgctcagcc ttgcttttcc ttacatattc
     3181 gttaatatac tgaacaattt tattgtcttt gaattctgta cgggtaataa actgagtata
     3241 ttgaagatta actgaatcct gctcataatt atcgtcatta gtaaattcta caaaaccaaa
     3301 tacagaaagt tttcttgcct tactatcatt gttttttatt ttcagcctcc aaacttcata
     3361 ggttttgttt agaggtacat aataaagaac attgctttcg atattatcat atcttgccag
     3421 catcgttgta taagctgttc catggcggca ttcactttcg tatttttcca agtcttttcc
     3481 gacaggctgc caggaagttg tccaataatc acttgtttct ttatctctta tataaatata
     3541 tctaccaggc tgatccatat taatggcatt aaaacggtat ctgataatac ggccatttgc
     3601 tccggacttg acaaaactat aacctccagc attatttgaa ataattgccc catactcagg
     3661 agaacctaaa taattcaccc atggagcagg agtattgggt tttgtaatga catattcttt
     3721 attcttttca tcaaaatatc cgtatctcaa aatcatcgct ccttaatttt gtaataatca
     3781 ttcaattagt aattataagc gaactcctga tgctattcaa taaattttct taaatgtcat
     3841 tgtttttatt ttcatttaac aatttcttta actcgtccat aaaggtatta atatccttaa
     3901 actgtctgta aacagaagca aacctgacat atgctacttc atcaatttct ttcaattttt
     3961 ccataaccag ctcaccaatt tgtgtgcttt tgacttctgt ttgcaaacta ttaaatatga
     4021 tgttttcaat gtcatcgaca atggcttcca tctgttttaa actgatattc cttttattgc
     4081 aagattttac tattccgtta aggattttgt ttctgtcaaa taattccctt gttccatccc
     4141 tcttaatcac catcatggga atggtttcta ctctttcata ggtggtaaac cttctttcac
     4201 aattttcaca ttgtcttctg cgcctaatgg aattattttc ttcagagggt ctggaatcaa
     4261 taaccctggt attggctcca ttacaaaacg gacacctcat ttcccacaac ctcccttcct
     4321 ctaaaaagcg gcaaaaaaaa taatattttg tgtatctaaa gtataaaaca caaaatatta
     4381 ttggtcaaat gcttaaaaaa ttatatttta atattcagat tccactagaa catccttacc
     4441 tgttacatcg accagaataa tatcttcacc tatctttcta attttatgcc atggaatttg
     4501 atattccatt tcttttccaa aaaagcccag gagcttgcca tggccaggaa ctataatttt
     4561 tttaatcctc ccttcaatca agtctatttc tatgtcacaa atgtaaccca aacgacagcc
     4621 atctatggta ctgatcactt ctttttgttt catatcatat atcctaacca aagctattcc
     4681 ccccagaatt tcaatcctct ctatacaatt atattcagca atttcattta gattctatca
     4741 ataaagatat ataaaacgca ctaaataact ttagtgcgtt attatataga ataaaaataa
     4801 aataagtgct taaatatact ttctcatatg cttaagtgct gtcttctcca gccttgaaac
     4861 ctgagcctgg gatattccga tttcttctgc aacttccatc tgcgtttttc cttcaaaaaa
     4921 cctgagagat aagatatgct tttctctttc gtttaatttt tgcatggctt catttaaagc
     4981 tatattttcc agccatattt catcttgatt tttttcgtca cttacctgat ccataatgga
     5041 aagggcatct cctccacttt catggtatac aggttcaaat aatgatatcg ggtcttgtat
     5101 tgcatccaaa gcaaatacaa cctcttcttt aggcatgccc agttcttccg caatttccgt
     5161 aatcgtaggt tcttttgaat ttttattgat taatttttct tttacctgta atgctttata
     5221 ggcagtgtcc ctcaaagagc ggctgactct tatggaattg ttatccctta aataccttct
     5281 tatttctcct ataatcatag gtactgcata agtagaaaat ttaacatttt gattgatatc
     5341 aaagttatct atcgccttaa tcagtccgat acaacccact tgaaataaat catccatttg
     5401 ctctcctcta ttattaaatc tttgtataac acttaaaacc aatctaagat taccttttat
     5461 atatgattct ctcgcctctt tatccccctg taatattctt ttaaataatt cttctttctc
     5521 ttcattcgtt agcacaggta attttgaagt attaacaccg catatttcta ccttgttgat
     5581 tgccataccg ttccctccag taccagtatt attactatta aaatgattac cagagagaac
     5641 taatttatac attgacttta attcattcta tttatttcct tttttaatct tgaaattatt
     5701 ttcttttcta atctcgaaat ataagactgg gaaatgccca gcatatctgc aacttccttt
     5761 tgagtttttt ccttaccttc tgtattaaga ccaaatctta attcaactat ttctttttct
     5821 cggcttgaca gtttttccat agcttttctt aataagtccc tgtcgatttc atcttcaata
     5881 cttctataaa ttatatcagg atctgttccc aatatgtcag aaagaagaag ttcatttccg
     5941 tcccagtcca catttaaggg ttcatcaatt gatatttctg tttttaattt attggtcctt
     6001 cttagataca ttaaaatctc attttcaata catctcgaag cataggtcgc taacttaatt
     6061 cttttgtcag gcttaaaagt attaatcgac tttattaaac ctatagtacc aatagagatt
     6121 aaatcttcta cacctatccc tgtattttca aattttcttg caatataaac caccagcctg
     6181 agatttcttt caataagaac agatttaaca tataagtcat cctttgttcc taaacgcttt
     6241 atcagcgatt cttcttcttc tctgttcaaa ggcggaggta aaatttcgct tccacctata
     6301 taaaatatag attctttaaa gccaaaaaaa tttaacagac gatatatgaa atatttgaat
     6361 tttaaactaa tcttatctgt aaaactcatt atttcacctc caaataacaa aaacgattaa
     6421 gcgatttcat gaaatatatc cggatgaagc aaggcctgat atttattatc tttggagagt
     6481 ctctgattgt atatgcctat tactacatct tttaaaactt taatggaatg atctttttcg
     6541 atgatttcta cctggtctgg tttaaaacct aaaagcatac cattcggtgt ccctaaactt
     6601 gaaaaaggta ttaccctgat ttttgaacgg attggacagc cactgatata ctttgtcaaa
     6661 agacctaaat cattttcttt tttttcatta aataattctt gaatgctctc aggaaaaaat
     6721 tttttaatag aagaaaattc tacgacaatt accggacttt gactaaaagg atcatataaa
     6781 gcatttcctg tatccattaa agcgcaagca tctattttca taccttcgta agatattttt
     6841 atttgataaa gagcccctgt atgattcgaa aatatacgaa tccaaatcaa aagcaattta
     6901 ataaccaaat aagaaagcag gctggatacg attaacaata taataggaaa attatctata
     6961 ttgaattgaa gagaattctt aatcatttga ccaacattta agtaataaaa gaaagcaatc
     7021 cctgcacctc caagaacaaa agccgttata tggaacagga taaaaacttt cattaacttt
     7081 ttaaaagttt ttgttttata tgtaatcatt aaaggaaaca tcggaagaat caaaatcccg
     7141 aaaaaattat atgattgatt aaaaatcggt acgaaaagaa ttaaacagta tattaaagca
     7201 gctaataaag caccaaaaat taatctttta tgatttactt tttcttttat tattttactg
     7261 acaatccaca aaataaaata atccataaca aaattgagaa ggaaaaccat atctatataa
     7321 atttctattg caaatccctg attcatgaca acaattcccc cattataata cttgcttatt
     7381 acatatatat tctttctgaa aaaatcatat tctccagtaa aaataacttg caacgatttt
     7441 aattttttaa tggtttataa gataattata caaataatga aacccatttt ttgtaaaaag
     7501 ggctgaataa ataaaaaaaa gatatgactt ttagtcatat cttttttaac cttattgatt
     7561 aaatttttat ttctttcttc tttgtaaaaa tacaggaata tcaatgaatg aagaatcatc
     7621 aggaacattg ttaatgactg ttgaactttt ttcttctttc aaagaaacag ctatactttc
     7681 tactttaggc accttgattg aaggttttgc aatcctgttt tcaaacccgg tagcaataac
     7741 cgttacgata acactatctc ctaagtcatc attaattgta gttccaaaaa taatttcagc
     7801 ctctggatcc actgcttcac gtattaagtt cgcagcttca ttcgcttcaa gtaaacctaa
     7861 attagagcca ccactgatat taatcaatac acccttcgca ccgtctatac tggtatccaa
     7921 taaaggactg tgtattgctg ctttggcagc aatttctgtt ttattctctc ctgaagcttg
     7981 tcctataccc atatgtgcga tacctttatc tgacatgatt gtccttacat cggcaaagtc
     8041 aagattaata atacccggat tggaaattaa atcagcaatt ccctgaacgc cctggcgtag
     8101 tacctcatcg gctttcttaa aggcttctat aatgcttgtc ttcttatcaa ttatatgtaa
     8161 cagcttgtca ttggggataa tgactaaagt atctacattt ttctttaact cctcaatacc
     8221 tttttctgca ttcaacattc ttttacggcc ttcaaaagaa aagggctttg taacaactcc
     8281 aactgtaaga attccttctt ccttagcaat ccttgcaacc accggtgccg cacctgtacc
     8341 cgttccaccg cccatgcctg ctgtaacaaa taccatgtcg gcgcccttta atgcttctaa
     8401 aatgtcgtcc ctgctttctt cagcagactt gcagccaatt tcaggatttg cccctgcacc
     8461 taatcctctt gtaattttct cccctatctg tatctttaca ctggcctttg aacgtcccaa
     8521 agcttgttta tccgtgttaa tagtaatgaa ttctacacct tttaaatttt cttctatcat
     8581 gcgatctaca gcattgtttc caccgccgcc tacgccaacg acttttattt gtgctacatg
     8641 gtcctgtgtc atgtcgaatt ccagcaccgt aaatccccct tcacttaaca attcaatctt
     8701 aacataatta tacactaaac aagaaacttt ttctattata atttaaacta attaacattt
     8761 taattaatta taataatata tatgatatac attaatatac tcttgttcaa gattatattt
     8821 tattaaaatc attatgtgag tggagaaaaa atagctttac ccttgtcaac gtttctcaaa
     8881 tctaatttcc ccatatcata aatatccata atttgaacaa gccattgaat ttttttatcg
     8941 aaatcttcaa cacttcctat tgaaacatct aaatttctca catagatatg aatattttcc
     9001 ggatcactta cactaacttt gacgacatct tgcagcatat catattttaa catcatttga
     9061 gacataattg ccacgacatc taaagcgttt ttatttttag ccggaataat ttcacctact
     9121 tgaaaatagt caaattccaa gccttccaca ataggtaaat tcttatcgta tgtcttacaa
     9181 ctctctaaaa cccgtccgtc tttgtctatg taaagatatg ttccaagata aggaacatat
     9241 cccactaact ttctttcatc aacttttatt tctaccgtat ccggccaaat aattctgata
     9301 ctggcagatt caatatatac atcttcattc agaatttttt ctcctgcctt tttattaaat
     9361 aaaaaaatat tgtctccctt attgacacca atcattgcaa tgatttcttc ttttgaataa
     9421 tgatcattcc catatatatt gatatgatgt atagaaaaca acggtgaact gaacaatagt
     9481 aaaaacaata ccacaagcat accaataaaa agtaatattt taactgtttt atgctgatgt
     9541 gtagattgaa aattgcccat cttaacaact tttcccatgt tttcaccatg ccttatcaac
     9601 taatcttttc tgatagaggc ccccagtata tttaaatctt tatcaatttg ctcatagcct
     9661 ctttcaatat gcttagctcc atcaacaatg gttactcctt cagctacaag tccggcaatt
     9721 attaatgctg cgcctcctct taaatcctta gcataaacat ttgtcccggt caaatgcccg
     9781 acgcctttaa taattgccgt ccgtccttct aaagtaatat tggctcccat ccgaatcaat
     9841 tcatccacat gcttatatct tgactcaaaa acagtctccg ttatgatact tgctccagaa
     9901 gcaaccgtta gcattgccat catttgagcc tgcatgtcag taggaaatcc aggataaggt
     9961 tgtgttctga tgatatcaat attttttaat ttttttggtg cctttaaata tattctatct
    10021 ttttcttcaa taatcctgca tcctgcttct tttaattttg aattaattgc ctgaatatga
    10081 ttgggattga cattggttaa taaaacttct cctcctgtaa ttgcagctgc tgccagataa
    10141 gtaccggtaa caattctgtc aggaatcact ttatgctcta cctgatgaag ggatttaaca
    10201 ccttcgatgg taatgacatc tgaagaagct ccttttattt ttgcacccat tccatttaaa
    10261 aaatcttgta aatccttaat ctcaggttct ttggcagcat tatgaatctc cgtcgttccc
    10321 tcagccaata ccgcagctaa cataatattt tcagtagctc ctacacttgg aaaatccaga
    10381 tgaatttttg caccaaccaa tttgggagct tcacagataa taaaaccatg ttcttctttt
    10441 atgctggcac ctaattgttt taatgcttta atatgcagat caatcggtct gggtcctaaa
    10501 ggacagccac ccggataaga aataatgact tttttatgtc ttccaagaac cgaaccgagt
    10561 aaaataatag aagaacgcat ttctctgact aattcttccg gtattttata agaatctact
    10621 gaagatgaat caatcattaa tgtcttatca tcccatctta ccttgcagcc aatactttcc
    10681 agaatacgaa tcatggtaaa aacatctaat atcctggggc aatcttgaat ataactgaca
    10741 tcaccattta ggaccgttgc cgcaagtatt gggaggactg catttttccc cccttgaatc
    10801 cgcaatgttc cctcaagaca ccttcctcca tctatataaa atctgcccat gaaaagacac
    10861 ctcctgaaaa cgtcaggtaa gcctatatat aactatagta tgcataatta cagaagttgt
    10921 gactaaggct gtatccaatc ctgttatttc aggagaaagg tactggcgtc ttatcttctg
    10981 tcccttcaaa aagctgtacc atggttacag gagaaaaatc taaattttat ttgttcttga
    11041 atatcttgat atattaagca gtattcctat agctgccata gtaaataaca aagaggagcc
    11101 gccgtaactg ataaaaggta ggggcattcc tgttgtcggt attgtattcg taatgacagc
    11161 aacattaata agcacttgaa tacctatcat acatacaatt cctgtcgcta ttaatgaacc
    11221 aaataaatcc ggcgcattca tagcagtctt tatgcctctc caaatcaaaa cgataaacaa
    11281 cgtaagtaaa actgcagcac ccaccaaacc cagttcttca cacacaatgg caaaaataat
    11341 atcattatga gcctctggta taaagcctaa tttttgtctg ctttctccta aacctaaacc
    11401 aaacagacct cctgagccta cagcatatag ggattgtatt ggctgataac ctttgtttgt
    11461 aggatccagc caggggttta gccatatttg tattctcacc agtctatacc taaattgagg
    11521 cagtaagact gccggtatgg ctgctgcagc aactggtgct gccaatgcaa aaaagtgcca
    11581 tactttaggg cttgccacaa aaagcattgc aatacctata aaagcaacga caatggctgt
    11641 acttaagtta gcaacagcaa tcaaaccagc tggtatcccg acgacaataa taagagttaa
    11701 gaataatccg gtaaaagtct ttaatttact tttgttacga gatatatata atgccagaaa
    11761 aaagattagt gtcagtttag ccagctcaga tggttgaaag cctatactcc ctattccaaa
    11821 ccaccttttt gagttattaa cttctttacc aataaacaaa accaaaacca gtaagacctg
    11881 agaaatcaaa tataaaagcg gagcccatac cttaagtctt ctgtaatcaa aattcattgc
    11941 aaaaatcatt gtggcaaaac caagcagaga ccatatgact tgtcttttta aaaaataata
    12001 catgtcattg aagttttcta acgcataatg ataactggag ctaaaaatca taacaacacc
    12061 aaataaggtc aaagtcacca ctgtgaataa tatggtaaag tcacactgtc ctcttactag
    12121 tttaacctct tctttttttc ttttggcttt tttataaccc ttttgagata cagctaaatc
    12181 cacagactca ccctctcaat gcataaactg cttttttaaa taacttccct ctttcttcat
    12241 aacttttaaa catatcccag cttgcgcaag caggcgacag taaaacgcaa tctccttttt
    12301 gggctgtttc tttagctaaa cggacagctt cttcaataga agaaaccttt ttaatattaa
    12361 aaaagccttt ttgattagct gtctttataa ttttatccgc agtttccccc ataacgatca
    12421 aagcctttac ttttccatca aatgcatcaa tccattcaga aaaatcagca cccttatcca
    12481 tacctccacc aatcaaaaca actggtgctt tcatggattg tattccaata atagcagcat
    12541 ctacattagt cgctttagaa tcattgtaat attctacgcc atcaatttta tctacaaatt
    12601 ctatcctgtg ctccacccct ttaaaatgaa tcacaacatc tctgataatc tctggcggaa
    12661 cacccatcga aacagaaatg gcaacagcag ccaaaacatt ttcaatatta tgatttcctg
    12721 gtatccttaa ttcctcggtt aaacaaatcg cctcatcttc tttacccaat ctgacccaaa
    12781 ttttattgtc ttttaaaaag gcaccttcat ttaattcttt tttcctgcta aataaaagag
    12841 cctttgcttt tgttttgtgc ttcatcttac tgcaaatatc atcatcgtca tttaaaacgc
    12901 aataatcatt ctcatcttga ttttcaaaga ttctttcttt ggcagcaaca taattttcca
    12961 aacttttatg tctgtttaaa tgatctggtg taatatttaa aatagcactc actttaggtt
    13021 taaacgtatg tatgctttct aactggaaac tgctaatttc tgctaccacc aaatcctctt
    13081 tctttaattc ttccactttc tctgtaaaag gtatcccgat attccctact acctgggttc
    13141 cgggattata ttttttcata atctccccaa ccaaggttgt agtcgttgtt tttccattag
    13201 ttcctgtaat cgctattacc gggcaaggac aaagaacaaa tgctaactct atttcagacc
    13261 agattggaat ttgaagctgt cttgctttgt ttaaaaattc taaatctgtg ggaacacctg
    13321 gacttaaaac aattaaatca aaagaatcta taatatcagt aggctcttct cctaataaca
    13381 tataaacagc cattgaatct aaaatatcta tttcttcctt aacagacttc atcaattctt
    13441 ccttactttt tacatcttga atggttactt ttgcccctag tctggaacag agttttgcag
    13501 cactaattcc acttttagcc attcctacaa ctaatacatt ttttttgcac cagtccattt
    13561 cttattcctc caactaccaa aaatctatta attcaatcct agaaatccta tcagacatgc
    13621 aacagctgtg gcaatgcaaa aaacagacac aactttgctt tcagtccagc cagataactc
    13681 aaaatgatga tgaattggtg ccattttaaa caccctttta cccgttgctt taaaataagc
    13741 tacttgaatc attacagaaa tagattctat taaataaatc attcctacaa ttgcaataaa
    13801 aattggcatt tttaatataa aagctgtaga aaccacaaaa cctcctaaag ccaatgatcc
    13861 tgtatccccc ataaaaactt ttgccggata agaattaaag agtaaaaagg ataaaaggct
    13921 tcctacaacg gctgctgcaa ctggaagaat actgctgttt tcaccccaac tgacaactgt
    13981 aaaaaatgta gcaatcaaaa cagtaacact ggaaaccaat ccgtccagac catctgttaa
    14041 attcactcca ttaaccgttc ccagcatcac aataaagaaa aaaggtataa acaaaatgcc
    14101 aaagtcaatt tctctgcctg ccgtaaaagg tatgataaca cgtgttccta ttttagcgac
    14161 atttagaata tagtatagaa aacaaccgct tacaaacaat tgccctacga ttttttggta
    14221 agctttcaat cctaatgacc gtttcagggc aactttgata aaatcatcta taaatcctat
    14281 aataccaaaa gataaagtaa ccagtaaaac ggctaaccca tccctgttgc ctttaataaa
    14341 taataaggaa gttaaaagaa tactgctaag aatgataata ccacccatcg taggggttcc
    14401 tgcctttttt aaatgacttt taggaccatc gtctcttaca aattgtccaa acttaagttt
    14461 gattaaaaaa ggtatcaata tcggacttaa gattaaattg atgatgaatg aaataagtac
    14521 ggcgtagatt gcttcatgat acataaacta ttccacctct tctaatttct ccactgtctt
    14581 ttccaaatgc attcctctgg acgctttgaa caatatcata tcattactgt caacaagcat
    14641 ttgaagttga ttgaataatt cttcctgatc taagaaaata aacactttat tcttatccat
    14701 tcctgcattc attgccgcca gagcaatgtt ttcggcttct cttccaaaag taaataaata
    14761 atctaaagcc ttcttggcta catactcccc aacttcttta tgcgcctgct cagagtatgt
    14821 acctaattca agcatatcac ctaaaaccgc aatttttctt ttaccttctt ttaattgaga
    14881 taatacatca agagcagctt tcatcgattg tgggctggcg ttataagcat catttataat
    14941 gtaaataccc tttttgtttc tggaaatatt cattctcatt tttgatgcct gatagttttt
    15001 gataccttct tttatctttt cttttgtcat tcctaattcc agaccaatag caactgctac
    15061 aagggcatta tagaccatat gcctgcctgg aactggaatt tctatttgca tttcattact
    15121 atcccataca atactggcat aagtaccttc taatcctttt tcttcaattt tattagcgta
    15181 aatgtcatta gaatgactta aaccaaaaaa gcagacagga acagttaatt gatccctaag
    15241 acttactaat aagtcatcgt ctccatttaa aaatgcctta ccctttttct tcatataatc
    15301 aaaaatttca ctttttgctt ttagtatacc ctctctgctc cctaaatttt caatgtggga
    15361 tgttccaata ttcgttatca cagccatgtc tggtctggca attttactta aattatgaat
    15421 ttccccaaaa tgattcatac ccatttcaat aaccgctatt tcatcttctc tggataaatt
    15481 aaaaattgtc aaaggaaccc ctatctcgtt attaaaattg ccctgggtct ttaggacttt
    15541 aaaactttga ccaagaacag aagcaatcat atctttagta ctcgtcttac ctacacttcc
    15601 tgtaacagca atcacaggaa ttgaaaacaa agacctgtaa taagtcgcta aatccataag
    15661 ggcttgtttt gtatcctcaa caagaataag tccttttcct tctggtatct ttatatccct
    15721 tctttggctt agggataaaa gggctccttt ttcaaaagca gcaggaataa aatcatgccc
    15781 gtcaaattta tctcctgtaa gagggataaa taaatctcct gcagaaataa ccctgctgtc
    15841 tgtagaaagg ctatcaatct ttatctgatg ttcatctata ttctttgaag gattgataaa
    15901 aattccttta accgctgaaa caatttcact aatttttatg gcttccattg taatcctcct
    15961 gtaggattgc tcttacttct tctgcatcat caaaatgaac cactttatct ttaagaattt
    16021 gatagttttc atgtcccttt cctgcaatca gtataacatc atctttcttt gccctttgga
    16081 cagccttttt tatagcttcc cgcctgtcaa cgattttttc ataggggcaa ttgctttctt
    16141 ttattcctgc ttctatctca tcaataattt tttcaggatc ttcagttctt ggattatcag
    16201 aagtaataat acaatatgaa gaatactccc ctgcgatttt tcccatgata ggacgtttac
    16261 ttttatcccg gtctccgcca cagccaaaaa cggttataat ctcaccctta gcaaattctt
    16321 taatcgtctt taaaacattt tccaatccat ctggtgtatg agcatagtcc actaaaatat
    16381 taaaatctga atgactgctg acattttgaa ccctgccagg aacgccttcc atcttttcta
    16441 aagccgtttt tattacttct tttggaatat ttaaataata cgcggctaca atagcaccta
    16501 aagcattata aacattaaat cttccaatcg tatttaattt aaaacgaact tcttcatgat
    16561 ctatttttac tgtaaattct attccttttg aactgatatg gatatcataa gccctgaaat
    16621 cggcttcttt ttctatagca aaagtgatta tttcacagct ggatgctttg ataatatcac
    16681 tggcactggc atcatccata ttaatgatgc catatttgca atttttaaat aatttcgctt
    16741 ttgccatttt ataattttcc atggtcttat gaaaatctaa atgatccaaa cttaaatttg
    16801 taaaaattcc tatttcaaag taacagcctg ccaccctgct aagctctaaa gcatgagaag
    16861 aaacctccat cacgcaatga ctgacttctt cctttaacat ttctgaaaac aatgcctgta
    16921 aatccaaaga ttccggagtc gttctttccg ttttcagtac tttattacct atacggtttt
    16981 caatggttcc taatacagcc actttattat tgtattcttc gagaattctg ccaattaagt
    17041 aagctgttgt ggttttccca ttggtacccg ttacaccaat aagcttaatt tgatttgccg
    17101 gatagccata aaaagctgca gcaacactgg ctaaggcttt tcttgtatcc tctacctgaa
    17161 tgaccgtaat atctgatgga agattttcaa ctttcttact gaccaaaaga cttcctgccc
    17221 ctttcgcaat ggcatcagga ataaatttat gtccatcaga aacataaccc tcgatacaaa
    17281 caaaaagccc cttttcttta acttgtctgg aatcataagc aatagattcc acttctttat
    17341 ctaaatctcc ctgaagtaaa cggtaatcaa tttctttgag caattcctta actttcattt
    17401 ttttcctcct aagctctagc atactgaatc attataccac aagcactcct aaaagctata
    17461 attttttgta aaaaacttct catttaaatt tattatggat cggtgtaaag aataacctta
    17521 ctattgggat tgacttcatc ccccgccaaa ggaaactgat cgtgtactat ctctccattt
    17581 cctattaact caacccctaa attaagtttt tttagctcat tctttgcgtc ctgaacattt
    17641 tttgaaatca agtcaggaac aacaattttt tctacaagat tcattttcat ttctttttct
    17701 gtgtattggg gttttatatt taaataaggt aagatatttt caaagatttc ttgtataacc
    17761 ggtcctgcaa ccgttcctcc ataataaatt ccctgaggct catcaataat gattaatgcg
    17821 attacttgtg gatcttcggc tggagcaaat gccaaaaagg aggcaatata ttttttgctg
    17881 cttcttggaa gcttttcaga agtcgcagtc ttccctccta tcctatatcc cggtatatat
    17941 gctttatttc ccgttccctc cgctacaaca ctttctaaaa ttttctttaa aacttgggaa
    18001 gtttcttcag aaataacttg ttttcctttt tcatactcaa aagtcttaat cacattacct
    18061 tcatcatcga taattttcga agcaaaatga ggtgtaacca aataaccacc attaacagca
    18121 gctgaagcag ccctgataag ctgcaaaggt gttatctgaa aagattgacc aaaagacatt
    18181 gtagccaatt caacaggacc gatatctttt aaatcatgca taatgcccac tgcttcacca
    18241 ggcacatcaa tgcctgtctt ttcatgaaaa ccaaattttt tcatgtattg ataatatttt
    18301 ttaggtccca ctctttctgc aacttccata aaaacagggt tacaagaatt ctgaacccct
    18361 tgtgtaaaag tctctgaacc atgggctctg ggatatctcc agcacttaat catcctgtca
    18421 cccaccagat gataaccttt acagtaaaaa ggggaatcga tattaactat tttttcctca
    18481 agacctatag ccgaagtgac aattttaaat gtagaacctg gttcataggt atcattaatg
    18541 gcaaaatttc tccacatttg atttaatgcc ttatgttgtt cttcaggcgt aaggctgtcc
    18601 catatagcct ctaaatccgg attgtttatt ttaaaaggct gatttaaatc gtaatctggt
    18661 ttattggcca tggcatatat ggcacctgtt tgaggattca tgataataat gcttcctctt
    18721 tttgcacctt ttgccttgac tgccttttct aaagcctgtt ccgcgtactg ctgaatattt
    18781 acgtctaaag tggtgactaa atgatagcct ggtacgggct ctattcttct ttcagggata
    18841 tcttttattt ccctgcctct gccgtctgtt tccatgagta ttttccccgg acttcctttt
    18901 aaatactcat catacttgac ttctaaccca ataatacctt gattgtcctt tccaacaaaa
    18961 ccaatcacat gggaagctaa attaccataa ggataatagc gcttcgaatc ctcatcaatc
    19021 tttaccccgg ctaagttaag ctttcttatt tcatcagcaa gttctttatc aaccttagac
    19081 tgtattcttt ccagagcaac atttttttcc acttttttct tgacatactc ataatccaga
    19141 ccaagctttt cagaaagcac ccttgtgact tcttcactat cctctacctg agcatggaca
    19201 acgcctatag tagccactgt ggcacttttt gcaagttcaa ctccgtttct atcatatata
    19261 ctgcctcttt gaggagatat tagtctgtct cttgtatgct gttcataagc cagttcctgt
    19321 aaaaaactcc catgagcaat ttgtaaatag gcaattctta taatcagacc aataatagaa
    19381 aaagtaaatg cacataaaaa aaataaaagc ttccgtttca tctttatagt gggtttatcc
    19441 atattatacc cccataattg ccagacttaa tatcatttta ttgcaattat ggaggcatta
    19501 ttctactatt tatcttattc ttttatttaa aaccggtctt tttctcatgc tattcttctg
    19561 taacactttc ttcttctgtc gtttcaattt cttctggatt ttctgtcaca tatagtttga
    19621 tggttgttcc taaaggtacc ctggtccctg gatttgggaa ctgcttttta acataattcc
    19681 ccattccaat ggcttcaaat ttaagtcctt taaccgttaa atctgttcct gtctctaaaa
    19741 gtgtcaaacc actgtaatca ggcaactcaa catctgtttt attttcgata ttggtttgac
    19801 ctgacggctc tatacccata taaggaagca cattttcaaa tatttcacga actataggtg
    19861 cagccgatac gcttccttcc gaataatttt ctgtttcatc caatatagcc aatacaacaa
    19921 tttgcggatc ttcaacgggt gcaaaaccaa taaaggaaag cacatacttt ccactgcctc
    19981 tgggaagttt ttcagctgtt ccggtttttc caccaaccgt gtaaccttct accctggcat
    20041 ttttaccggt tccgtctgtc acaactgttt caagataatt tcttagaatt tgagaagttt
    20101 cctgagaaat gacccttctt tttataactg gacttgtcgt cttgacttct cttccatctt
    20161 cagtcacaat ttgctttacc acataaggct gcatcagatt tccgccatta ataatagccg
    20221 aaaatccagt aatcaactga ataggcgtaa cattaaatgt ttgcccgaaa gaactcgtgg
    20281 ctaattcaac tggccttaaa gcttcttgtt tatgtaatat tcctgattct tcacctggaa
    20341 gatcaatgcc tgtaacttct ccaaatccaa acaatttctg atatcggtaa aatttttcag
    20401 ccccaagctt ttctacaata tccatcatgg ccacattaca ggaatttgct aaagcttgtt
    20461 ctaaggtttc ttccccatgg ccccaggttc tccagcaatg aatggtttct gtatgaattt
    20521 ttttatgacc tagacaatta tatttatcat gaactgtaat cgagttttct tccaatgcgg
    20581 cagcaatcac aaaaggttta aacgtagaac ccggttcata agtcacactg agattataat
    20641 ttttccatac cgtatttaac attttcagct tttcttcatt cgataaagat tcccagatac
    20701 cttcatcaaa atattcactc aaatcattgt atttattggg gttaaaagac ggataagcgg
    20761 ccatggctag tatttcacct gtttttggat tcatagctat aaccgctgca tttttgggat
    20821 tatgttcttc tactgcattt tttagtgcca attcacaaaa atgttgtatt gtctcatcta
    20881 tcgtcaatac aaccgtatgt ccctgttgag gcgcaatatt ttcttccatg acatattttc
    20941 cttctttaat catagggaaa acccgtcctg aaacgcctct taaccaattg tcataatact
    21001 gctcaacccc atattgcgct tgtttgtctg cattaacgaa acccagcaga tgagcagcaa
    21061 aatcgtttct tacataattt ctgaaagaat cttcctctag ccaaaccccc attaagccct
    21121 tttctctgat tttttgcgct gtatctgcct taacatgttt ttttataatc tgataccttg
    21181 attttgggtt tttttcaatg gtctgctgaa tgctttccgg agaaagattt aagatttgag
    21241 ccaaatccgc aactacttca ttcctttttt cttcagtaat gtgctctgct aaaatgttag
    21301 gatccaaaat cacattgtaa acaatggtgc taatggctaa atcgtgaaaa tttctatcca
    21361 caataaagcc tctgttagga tatataacct gctctgtatt cagtctttgc aaaactgctc
    21421 tttcttcata ttcttcccca taggtatact ttatatatcc tatccggtag attaataaaa
    21481 ggatagaacc tattaaaatt gtaagaatga taaaatttct tcttctgact tgtcggatta
    21541 attcgttcat tgcttcacct tttcaattta aaaaaatcaa aaaaacttgc agcaacgact
    21601 tcatcttcag tgctttgatt taaacggttc tgatcttgtt ctagttgatt ataagctgtg
    21661 taactcactt tgggtatttc aatataaaca atttgatgcg ctgcaggttc catcattcct
    21721 aatttttctg tagccacttc tttgatataa gttaaatcaa ctgtttcagc aatctcggat
    21781 tttaatagca tatttgaatt ctggatttct tttaattccg attttaattt ttttatttct
    21841 ttttgattat aagtaatgga ggcatattgt cccatgataa acaaacatcc tataaaaagg
    21901 cacaaaatat tccctacaag tttcaattta taagatataa cctttctggt cctctgagct
    21961 ttttctttct tttgcttata atgaagaagc tctttttcat cttcctggat caatgtcttg
    22021 tgggtatcat tgaatttggg tgcagaagtg ccatataggt attgttctct aatcctattg
    22081 ctctttgaca tattaacctc cattccgcct gatggccatt attataaaca atccataaat
    22141 ttatgctttt aagcgttatt cattataatt tttctaaaat tcttagttta gcactccgtg
    22201 aacggggatt tttttcaatt tcctgcggac ttggtaaaat tggtttcctg ctgataactc
    22261 tggccattgc ttttttacca caaacacaaa taggaaaatc aggagggcaa gtacaaggat
    22321 tttctaaagc cttaaagctt ttcttaacaa tcctgtcttc taaagaatga aaagtaataa
    22381 tacaaagcct tcctttgggt gctaatacgt ctacaatatc ctttatggac tgttctaata
    22441 tctttaattc cccattaact tctatacgaa ttgcctgaaa agttcttttt gcaggatgag
    22501 gtccttcttt ccttgctcct tgtggaattg ctttttttat aatatctact aactcaaaag
    22561 tagtacttat aggggctttt tttctttcat tgacaataaa ctctgcaatc cgcttagccc
    22621 accgctcttc accatattct tttatgattt cttctaatcg tttttgacta tactcattca
    22681 cgatttccct ggcagttaaa gcctgattcc tgtccattct catatccaaa ggagcattat
    22741 gcatgtagga gaaacccctg tctggcgtgt ctaattgata agaagatacc cccaaatcca
    22801 ggagcatccc attaatcgaa gaaagtccta aatcgattag tatctttttt atatttgaaa
    22861 agttgtcgtg aactaaatct acatttttaa attggctttt taattttatt tccgaagctt
    22921 taatggcatc aatgtccctg tcaattccaa taaatcttcc gtactcattt aaatgtttgc
    22981 aaatttcata agaatgaccg gctcctccca tggttccatc tacatagata cctgaaggat
    23041 cgatatttaa tccttctatt gtctcctcca ataacactga tatatgatgg aaatccatag
    23101 agattcacct cattttatat tcctaattcc tgcatatttt cagctaattc actggcatca
    23161 aaatcttcat tattatattt atcccaattt tctttactcc atatttcaac cctgttcgta
    23221 acacctatta aaatcacatc tttatctata ccgctatagt ttcttaaatt atttgggatt
    23281 aatattcttc cctgcttgtc cacattacat tcaaccgcac ctgctaagaa aaatcttaca
    23341 aattttcttg cattggcatt tgtgataggg agagttttta gcttttcttc aaaaattttc
    23401 cattcagaat tagggtatac aaacaagcaa ttatctaaac cttttgtaat cacaaaagta
    23461 tctcccagct cttccctgaa ttttgccggg ataatcagac gtcccttatt atcaattgaa
    23521 tgttggtact cccccataaa catatatata tcccacctta gtggtatcta tctcccactt
    23581 tccaccacaa ttaaccactt cttacctcat attgtagtat gaccgtttta ttttttcaag
    23641 tacttttcgc aaaaaaataa aaagagatag gaaaaaattc ctatctcttt tatgatttat
    23701 gctattatgc atctaacgaa cgtgtgttac atgatatgtg atcatagtag agtaaaatta
    23761 gtctatctaa ctccgtactg attctgtaaa tttcctgaaa atcattctcg cctacgatta
    23821 gttcgtttaa cttttctcgt agaagattaa tttccccgta aatattattc atttcttcac
    23881 cccgtttttt atccccattt ttttgaaaaa atgctttttt ctttagatat ttttttatta
    23941 tcttacaaat attataatac ttttccaatg ataaatcaac atattattac acttttttct
    24001 attttttaat ttcttttgta tttttttcta ttattgatta ttaaaaagat tgaacaaacc
    24061 actaatagaa tagataaaag ttgtgatacg gcaagacctg tagaaccaat ctgaagctga
    24121 tcgatacgca gaccttcaat ccatgaccgg cccagcccat agccaataag atacatgcaa
    24181 aatacttcac cattaaattt tttccttttt ttataccata aaaggaaaaa taaaaccaat
    24241 aaattccata gacattcgta taaaaaggtt ggatgaacct gaatatattc tataccttca
    24301 aataaaatag tattatttaa aatatctggt gtaagatatt gattcacatt ttcttttatt
    24361 aaacgcatag caaataaaga atccgtatat cttccaaaag cttcctggtt gaagaaattt
    24421 ccccaccggc ctatagcttg tcctaaaatc aagctgggtg cagccgtatc tgcaaaaagc
    24481 ccgaaagaaa tatttttctt ctttgtaaat atccaagcaa caataactga agtaataatt
    24541 ccaccatata tagccagccc gccctctctc agggcaaaaa ttcttaacca attgtccttg
    24601 taatcttccc aggaaaaaat aacataataa agacgtgcac ctataatagc agcaatcaaa
    24661 tcatataata aaaaatctac ataataattg ggatcctgat tggttctttt tgcttcctcc
    24721 atagcaagta aaagaccgca agttacagct aagccaataa taatcccata ccaatataca
    24781 gaaatcccaa aaagtttaaa agcaacagga tctagttttc caatttttat ccctatatga
    24841 ggaaaccaaa cttctggtgc agacatatat atctcctttt cttaagtaat ataaaccgct
    24901 tttaaattta tatacaagtt ctaaattaaa ttatatatta aaatgctgtt tttttcatcc
    24961 ttttatgaaa aaaatttttt attggtctta gaaaatgaaa ataataaaaa tgtgatacag
    25021 actgtttttt taatttattg ccacccattc agccttaatc ttatgcaaaa caacgattaa
    25081 aacaatcagt gctattacaa ttataacagc aaataatttt ctaaaaatga ttaaccttcc
    25141 ttttggcatc aacaataaat tgaaaaaata tttcataaat ttactccgta taaattcttt
    25201 tgaaactatc attattatat tacttttcta tttattgtaa atcatttcat cacatttttt
    25261 aatgttaata gataattaat ctttaaaatt ttcgccatta aaataaaaaa atatgtctta
    25321 aacattgggc acgatttctc tgataataga agataactca ttggctaaac catcaatact
    25381 tttcatgttg tttatactat ttgttatttt attcattctg tttattattt ctggagatgt
    25441 actaattcca atatttttta aagataaatc tgtcgaatag actttttctt caattttcct
    25501 ttttaatctt tcaatttctt ttttatccag ttcatgaaca gtatctatac ctattaaagc
    25561 agtattccct gttattatta cagtcgtttt attaacttca ggaaaatcat ttatcagctt
    25621 agctattctg tttgcccttt ccttattata accaatatca aaataatgat tatttatcct
    25681 ttcaacatca tcaggaatgt tatatggata agaacgatta aattcctgaa tagaaccctt
    25741 tgctgtgatg atatcttctg ccgtattatc tcttctgttt aatctttcga tactattttc
    25801 aatataataa ccgcttatat tatttctcat gttctgacat gctgatatga taaaaataca
    25861 aaagcaaaga atcatcaaaa tatttttttt caatgggatt tcacctccat tattattgtt
    25921 tgaaataatt aagtttttta aagtagaaaa gaatgtcctt ttcaccactg ttttcatgat
    25981 ttgtttttta tatacgaata aaaagccaaa ttattaaaat actaatgtta attccattta
    26041 ctatagaatc tttttctaat tttctttttt catccatcaa agtatgataa aataatataa
    26101 agttttataa tagcttatga tatatcagaa atatcataag aactaaggag gtaataatat
    26161 gaacgcaaga actcaacaac gacattacag agtagctcaa aaagaaatag aacatgcacc
    26221 aaataaagga atcttcatcg aaaacgataa aaaacaatta tacgcaatta ttgcaggcgt
    26281 tattcttgtt ttaactttta ttcaaggact gattatcgga ttatttatta gtagagatta
    26341 aaaagcacaa tgtttacatt gtgcttttta ttgtgaaaga tttttagtta tctcattgtt
    26401 ttccctatgc catgatttat tgattgatta atctaaaatc tttaggtata atttatactt
    26461 ggttgttttt atagatgctt taatcataaa tgaaaggaag taatatatat gaaactagga
    26521 attgttggtc ttcctaatgt tgggaaaagt accttgttta attctcttac caaaggcgga
    26581 gcagaatcgg ccaactatcc cttctgtact attgatccga atattggtat tgtagcagtt
    26641 ccagatgaaa ggttacaggc tttagataaa atttataatg cggaaaaagt aacccctgct
    26701 attatagaat ttgttgatat tgctggtctt gtaaaaggcg caagtcatgg tgaaggtctg
    26761 ggcaataaat ttttatccca tatccgtgaa gtggatgcca ttgtacatgt ggtcagatgc
    26821 tttgaagata caaatatcgt ccatgtagaa ggttctattg accccatccg tgatatagaa
    26881 accattaatt tagaattaat cttttctgat attgaaattc ttgacagaag aattcaaaag
    26941 actgaaaaag ctgctaaagc cgataaaggc ctcttaaaag aactggaaat cctggaaaaa
    27001 ataaaaaagg ccttagaaga tggaaaatgc gcaagaaacg taccattgga atctgatgaa
    27061 gaaaaagagt ttgtttcttc tttaaatctt ttaacttata aacctgtttt atacgcagcc
    27121 aatgtcagtg aagaagattt aatttcaaat gccgaagaca attcttatgt taaaaaggtt
    27181 aaagaatacg ctaaagctga aaactcagaa gtctttgtta tttgtgcaaa aatcgaagaa
    27241 gaaattgccc agctggatga agaagaaaaa aaattattct tagaagattt gggcatcagt
    27301 gatacaggac tggaccgctt aattgctgcc agctataaac ttttaggctt aattagtttc
    27361 ttaactgcag ggcctaaaga agtccgagcc tggactattg taaaaggtac aaaagcacct
    27421 caggcagcag gaaaaatcca tagtgacttt gagcggggct ttatccgggc agaggtgata
    27481 tcttttgaag atctcattgc ttgtggcaat tacaccatcg caaaagaaaa aggccttgta
    27541 agactggaag gtaaagatta tgttgtacaa gacggtgacg tcatcatatt tagatttaat
    27601 gtataaaaaa aagaatcagg ataaatcctg attctttttt tatagtattt ttatatttct
    27661 ccttcagtcc tttcctcttt ttcttgtgca tctgttttgc attgcttttt cttcccaata
    27721 ttaaactttg ataaaagttc aggaatcata tcaattaatt tattaacact atcctgattt
    27781 ttaatattta taagttgggt tgtcccatcc ttaatgagca aaattgcact gggagtaatt
    27841 tttgcaccta aaccacccat gccggaatcc ttatcttccc ctttatctgt cttccctccg
    27901 gaagcacctg ctcccacgcc aaaacataca tctactaaag gaagaatgat tgcatcttct
    27961 acttttattg gttctcctac tactgtcttt gttgatataa aactttcaag ctgactaaac
    28021 aaagattcta aattcgtctt taaattcgat tccataccta tcatcctttc atgaagataa
    28081 ataatttttt acaatttctc ttatgggctt tttcaacaag tagttcgcaa gatatatgat
    28141 aataattcct atttgaaaac taccccttgc ttctagttct ccctgcaaaa tcttttcatt
    28201 aaagttagcc ttaatatgta cactatttcc taaaaagggg cttattattc ctattgctcc
    28261 taaaagatac cctgtatgag aagggtcgtc cagaccaaat tcaagatagg atgaaaacgt
    28321 acgaggtctt atatgaatga atattttttt aagaaattga taagtataca tgataatttc
    28381 ttttttattg ggataatcca gtagatttct caaagaaaat cccgcatctt ctgcttcctc
    28441 aattacatct gtctcaacaa tctcatcaat atcattgtat ttattaacat cttcgctttc
    28501 cagagagttt ttatgacttt taacaacttt ctttgataca tcattttctt ttgaaaagtc
    28561 gttaatgctt ttttctttta ttttatactt cgtttcaatc tcttcattct ttattctatc
    28621 atcctttttt tcttctttgc tttccagttg aatattctct atcgctccgc ttgtctttcc
    28681 ttgctttttg attttatctt tctgcatgct ttcctttttt actcttcttt gctcattaat
    28741 tattttcttt gaaaacaccc tgaaaatttt agtttctttt tttgactttt tcatcacaaa
    28801 acaataataa atccaattaa aaatccatct gattttcaca tttacatcta tagcaaaatt
    28861 cccatcagca gaaagataaa caaagtaacg aaagggaata aataaaagca gcagtaaaat
    28921 taatattaaa atacccaaca gagtagccag tataatccct atatatttta agacaagcaa
    28981 taaaacggat attattgaca tcacgattgg ctcctattcc catgcaaaaa attcttttat
    29041 catcatagga tctttgtact ttttataaac atcatcaata attcttcgtg ttaattcaaa
    29101 agcttcttct ttattacggg caattccaac aactttacaa tctctgtaac gactgatatt
    29161 gtttttcatt tcatttgttt tgattataac cattaaatat tctgaagaag gctctaaaca
    29221 aagacaatat atatctttat agagatagtt tttaataagc ctctttacga ttctctcttt
    29281 attttttatc gaatcgccag tgtaataatt ttttgctact ttcataggca ttcttctctc
    29341 atagaaatat gaaaatcttc ctattttaaa tttttgacta attaaaatat attataacaa
    29401 aaataaaaaa taaagtcacg atatatatcg cgaccttatt gtaacgctgc ttctaacttc
    29461 gaaatatgct cccttggaaa tactcttaat aattccatat attgttcttg ggttaatcct
    29521 tctgtagata tataaatatc tatcatttca tctactgtac tgctttgaaa cttcttcttt
    29581 aattgctcaa attgtgtttg ttccacagta taatcacctc ataaactttt tcttttttag
    29641 tatatgcttt atttattact ttattcttat taaagaaatt tcttgtctat taaaatcatt
    29701 aatttacttt attattacaa ctgaatagat ctgcttaaat tctaaat
//
LOCUS       DAOSUD010000020        28380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1099_length_28380_cov_25.154316, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000020 DAOSUD010000000
VERSION     DAOSUD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 28380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28380
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_28380_cov_25.154316"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..2220
                     /locus_tag="PLG49_04240"
     CDS             184..2220
                     /locus_tag="PLG49_04240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ16562.1"
                     /translation="MTTKEVTEKFLKIRDQLIQRDFKNLNESQRQAVLKGKGPQMVIA
                     GPGSGKTHVIMHRLLYLMTYGPVYHTNLIPQGITKNDLEIIKEKPESAQARSLLYSFA
                     VRPENILVISFTKAAADEMKTRFSFLASQHKLDYGKVIFSTFHALFFRILRSVYGYSL
                     KEILEENEKRIIFRQIAEALKIEFEDEEEFISELQNEISLIKNDLIDISYYNSMILPS
                     EQFKNFYLTYDSYKRKNNKIDFDDMLYDCYDLLKTNNKVLDFWRNKFKYILIDEFQDI
                     SKVQYELIKLLAAPDNNLFIVGDDDQSIYKFRGARPEFLLNFPEDFKNAGKVILNINY
                     RSTKKIVLASNKVISKNKKRFEKKITGIHDEGADIVLIQSEDIENEALHIVEWIQSLT
                     RKELAYKDIAVIFRANLQARALVDIMFDLNIPFCLKDEIRNIYEHWIAKDLMAYLRLS
                     LDDYDNESIERILNKPKRYISKGLIFEAKKKKGNLLDSLYYLPGIRGWQLEKLDELKF
                     HLSRLKKKAPEEAIAYIRKNIGYDDYILEYASYKNIGTRGLMEVLDEIMEGAKSFKNC
                     TDWLRHIGKLEEEIKKGKKRGVGQNDGVVLSTMHGAKGLEFEAVCIAGAVDGIIPHYK
                     SQGISGLEEERRLFYVAMTRAKKYLALSTVKKRYEEEVKPSCFIEDLKEDLKAK"
     gene            2285..4786
                     /locus_tag="PLG49_04245"
     CDS             2285..4786
                     /locus_tag="PLG49_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16563.1"
                     /translation="MLYSKKRRGKQKSISIILVVLLILAIILSSILPVIVYGEEKDNL
                     KIKGEVGFKNKYIINMNTPLILEITNEGKEFNGTVQVKVLTGDHEIKEYVIYEQNITL
                     PQGSTKKVTMNIKPVSLQKYFTVSLVSDSKSKEVAKKIIYATPFEPYRGFVGVLSDDF
                     ESVSYLKNLKLDTARSAFIGNRLVELTEETFPENLDLFNNFEMIIINNFDTSKLSDEQ
                     LDNLKTWVLSGGVMVLGTGVNASKVLRGFDKDFINVKDNGMISINDFAQMEEIGLHAL
                     EVYAMDVADLEIENGTGILFAGNLAISSLINRSEGSIVVHHFDLGLNPIAGWQGNYYM
                     LTELYMNVPDVIEFASQDRDSYYSYNYDYYNYSLRLFPRPNQNYLFVIAGIIIVIYIF
                     LIGPILYIILKIKDKREYGWFIIPVASVVFSLLIILISRASILRHPLASSVGVISLGE
                     GETRGTMEVSMGVFTPKSGENVIEILKGIKPQIGSFYYYYNSYNTDSKKEKIIGKVNI
                     GEQDKITFYNTNSWAMNKITGKTKFDASGPVNATIYFKDSELVGVIENNLGFDLEYCI
                     LVFGNNYRYIGKIENNKSVNIDVALKYNTSNKYDVLNQIFGITFDKKDLKEKWGDIPE
                     EELQEIIQRRHIYEESFDRYYNYNTMQYYGQNSSSYDYFKDNYVVLYGFNDEELLEGI
                     KVNNHLVEHYKQNLFVIPLKIDFENMDYIEIPYGFIKPFITSEAGFSYDNYDDSVWIT
                     SDGAVDITFPVPSDLDVKEFQVQYSETINVEKALIYNNVTDEWEELSALPYSDNAEDY
                     ISDESMIQLKFEIQLVNDVPDARLPVPYIYLKGDK"
     gene            4788..5711
                     /locus_tag="PLG49_04250"
     CDS             4788..5711
                     /locus_tag="PLG49_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16564.1"
                     /translation="MLEIKNLYKRYGKFLAVNNLSLSIAEGEIFGFVGPNGAGKTTTM
                     KIVAGLLKASGGQVLINGINVISHPGKLREKIGYMPDFFGVYDNLKVDEYMDFYAGMY
                     KIPYKDRPAIIDNLLEIVDLSHKRDAYVDSLSRGMKQRLCLARSLIHDPDILILDEPA
                     SGLDPRARIEMKEILKQLRTLGKTIIISSHILPELAELCTSIGIIENGQMVACGKVNE
                     IMQQLTHKRIIKIRLLDKMEELIRILQEQPDIHNIFEKTDEVEFEFDGDDIRLSQLLK
                     QIMEKDVLLTAFHEKEGNLEDIFMKITKGGE"
     gene            5713..6561
                     /locus_tag="PLG49_04255"
     CDS             5713..6561
                     /locus_tag="PLG49_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ16565.1"
                     /translation="MMINPVLRRELKTKLRSWKAPVLLMVYLIILTGFGGLVLLLTLY
                     TSYDNSFNPQDTLTVYGLLAGCQMGLIILLVPALTAGTISGERERQTLDLLKVTKISS
                     FSIIMGKLLASISQIMLMVLASIPVFSIIFIFGGVSLKNIALLFLYYIVISITLGSVG
                     IFCSTYFRKTTVATVVTYLFILIFCFGSLIVYAIVQEFVYYIFSYSDGLTYFQSVLLI
                     GGNPFLGFLSVIENQLGTTDTLYYFLNAHNNPLNINIKLWQFNLVFNCLVSVLMIFLS
                     VLKIRR"
     gene            6582..8108
                     /locus_tag="PLG49_04260"
     CDS             6582..8108
                     /locus_tag="PLG49_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16566.1"
                     /translation="MKELYRALKPLKRKLILEKWLHLILLSTIIAMGLNIGLLILSKF
                     TLISYIYLKFIGIIILSLFTGSLTLLFCLPDMRKLVNKGDSLGYKERFVTAFEILSHK
                     GKQLNSFEKMVVEDAIEKARKANFKREYHISLPEKKLKVLAVMLIGAMIVGFAPSPVK
                     EKLEYQMRLKEVIEEKTKEIEKVQNELKENKELTEAQVKQINKELDELKKNIKQAKNE
                     ADIVRARQKTQEELKRISKDSVQRDLKKIGESLSQHELTRELGDQLQKGNIEDIKRSL
                     EELRQELANMDQNQLKELANAFKEAAESLESDSALRQALSDYQEAITNGNLSDLQECY
                     QQLSEQLAQRASENEDLRKAIEKINEAMNKDNNLSQNQGQNQSQGQNQNQSQNQNQGQ
                     NQGQGQGRGQGQSQGQGQDQGKGQGQGRGRGHVPNENIYSRQAEDKEDFDAYVEGQKN
                     QGGQFHQKEEKMIGKKGESVPYQEVFQEYKKEALNLLEDDNIPYGIKNLVKEYFTSLE
                     "
     gene            8236..10047
                     /locus_tag="PLG49_04265"
     CDS             8236..10047
                     /locus_tag="PLG49_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOQ16567.1"
                     /translation="MKFYNPWAFLGLVSIPIIIVMYILKQKYEEKKVPSLFLWEKALS
                     YSEAYMPWQKLRKNILLFLQLMLAGLLVIILAKPYIEGGMIKSESYILILDQSVTMQA
                     KDESPNRFEEAKNKMYQFINNLEPAAKISVVVMGKEPFILANQSTDKNAVIKKIEDVK
                     VLNSGINLENTETLVKAINDNIKSSIYIFSDKDIKMTDLNSNLIKVGKHYENCGITLL
                     SHAKEEEQIAALVKVKNFGNENNELSVALYGDEKLIDIKRVELAAKEEKNVFFTGISK
                     EIKVLKAQIDLEDILEVDNVMYDVVYEDVKEKVLLLTRQNIFLEQILSLLPQVELYKG
                     DLDSMDNLQGYYLYIFDGYMPEKLPEDGHILVFDPPLENPLLEVKQDIEVSTLYPSGG
                     ELLAFLDQIKFDIAKTKKLSVPQWGQVILNSEETPLIIAGERGEQKIMVCGFDLHQTD
                     LPLRTEFPIFIYNTIQWFIPEKIHNLEKIIAGDTIEFQIAPESKEVSLVKPDGTYERL
                     APPFPLQPYKNTNETGIYTLEQKGEKIDSFHYFAVNPLVEWDFAGSFAESNGEDAKEN
                     SQTYDNMIEGNRNLKNILLIMLLVLMFLEWWVYVRGI"
     gene            10037..12808
                     /locus_tag="PLG49_04270"
     CDS             10037..12808
                     /locus_tag="PLG49_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ16568.1"
                     /translation="MVFNFINTRYLLLIPLFIFFLYFTSKKLTLSSYKKSFLLCLRSI
                     IVILLIFSLSGLQVKKIVDKTTTIFAVDLSDSAKSAIKDAEAFIKGAMAFKGSHDQVG
                     IVAFGSNAGVEASPSQEPVFTGFSSILNSNSTNIAEGLQLASTLIPEESKKRIVLISD
                     GKENIADALSQAKLLASQNITVDVLPMTNQIEEEVQLTELNVPKYLSKNQQFTVQVTV
                     DSLIDTKGVLKIYNGKNLVESQEVQIRKGQNRYAFSDLASDGGSRIYRAEIEAVVDTL
                     LQNNVAYAYTYVEDIPHILLIEQENSGAEIKEILKNSELVIDVVNPFTIPQEADLLAQ
                     YDGIIMANISADDLTEKFLNNLENYVKHTGGGLIVSGGENAYALGNYFDTPLETILPV
                     DMELKDKTKIPDMGIVLVTDRSGSMMEAPYGVSKLELAKEAAIRSTDVLNEFDKIAVI
                     AFDDQPQVIVELQEVKGNLGAIQDKIAGISIGGGTSIIPALQKAYDILSDADTKVKHI
                     ILLTDGQAETSGYDDLISQMKLKGITLSTVAVGRYSDVVLLERLAREGGGRYYYTDEF
                     SDLPKIFMKETMTASKTYIQNETFYPKLTRLSPIVEGIDTLPPLHGYIATSPKSRAEV
                     IFSSEKDDPVLAVWRYGLGRTAAWTSDMHNQWSLDWLASSSGVEVFRNLVSWIIRKPM
                     SDSVMVEGKVEGENIRLKASIPNMGSEAEIKVNVVAPDMTEHEIYLKASAPGEYTGLF
                     PGNNIGAYILNFQVNQNGEEESIHTGINVPYSPEYDINKITQDTGLLNQIAHITGGRV
                     LTQPEEVFKEIESTIYGERDIDDFLLILALLFILFDIAFRRIGVINNKVEEIIISVIQ
                     FFKNKKKKEKKVLIVKEKNKAPEKTSVPKVKKEKKKEDKKEKEEVKKIENTTSALLAQ
                     KRKRTSYKK"
     gene            12888..14012
                     /locus_tag="PLG49_04275"
     CDS             12888..14012
                     /locus_tag="PLG49_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ16569.1"
                     /translation="MVEEKMKILKMLEEGKITADEAVNLLNSLDGKSKPNLDRGYESK
                     NMQNNTQPKNKTESSFTMDLEKKIEVLSRDLEPKIKKAAKTIMDTADSFAEKIAKSFS
                     YSPAGPQSNDMTFEMYVSKGDNTQLRLKSKNGPIHIKGYNGDKITAKIKYSCKGGSQK
                     IELLKVDNTYYLNYDEANFTFVAIEAFVPEFLFSRVFFESNSNVYVDALSGDEITILT
                     NDGSIELKNISAKNMKLESARGNILLENITGELLRAESCNGYIDVKLSDIAKMDLLTS
                     NGEINIDSSIRELKAANYYEWNAETSNAAIKLNSFRGFDIGYDFKALTSLNGIHVHIP
                     SVDFIENEKNFVKGMTKNYPSASKKVKINLETSNAPITLY"
     gene            14203..14931
                     /locus_tag="PLG49_04280"
     CDS             14203..14931
                     /locus_tag="PLG49_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOQ16570.1"
                     /translation="MSSLDDKLYEKTVECPVCGNVFKVTAVKRKAYVVESRDTDFCVK
                     YKDINPLFYDVWICQLCGYAAQKSTFSKITYKRSQLIKKHITPRWVAKKEPAVIEYEA
                     AINKYKLALINAQISKAKDSEIAGICLKIAWLYRFMGKKENELHFLNHALNKYVDAFS
                     KEKFPVENMDEATVMYLIAELYRRVGNFEESARWFNKVISQRGIPERILNLAREQWSL
                     LREDLKNEKSKSQKNNDEVSEALK"
     gene            15032..16015
                     /locus_tag="PLG49_04285"
     CDS             15032..16015
                     /locus_tag="PLG49_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOQ16571.1"
                     /translation="MKVGKIPPNLLKEYVFSNLKNHKKEIILGPGIGEDSSAIQLAED
                     EIFLVSTDPITGADKDMGYLAVHVSCNDIASCGGEPIGVLLTILLPEASDKEDLIRIM
                     EDANRASKEIDVQIIGGHTEVTNSVNRPVISATAIGKVKKDKLVRTENAKVGQDLIMT
                     KWAGLEGTAIIAKDCREELLKVFSEDFLNSAQNFANYLSVVSEGKIAADFGATSMHDA
                     TEGGVLGAVWEIAECSGVGVEVYLDKIPIKKETKDICNYYNISPYQLISSGCMIITTY
                     DGNRLVEQLKMSGIEAHIIGKITEKDRICIQNNEVLPLRQPDVDELYKVIK"
     gene            16044..17732
                     /locus_tag="PLG49_04290"
     CDS             16044..17732
                     /locus_tag="PLG49_04290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ16572.1"
                     /translation="MNLKERKRIENILTRFRLVAFLFVFFMVPLNISMSRQMEALLLN
                     RMFVFITYYLISVGIGFLLFKNIKNEKMFDLAIGISTVFDFIAIPWAATVILPVCDLP
                     IEVVYIIVQMTMLIRYRTNFYSLIGNALVLLNIWLYELTIGASFYWRFWSIRGLFWTI
                     IFGFNLIIFWKLLKNYDELLEQKELENITIEDSKRSIDNLIHINKISASLNESLSQNE
                     IIKILLSNVSKMVNSEDCAVLLYSKKETDGYLYNYRKFKESSSNNREIIKYEIINIGD
                     EIKKIKSMKDYKHVIGARQPFYMADLVNIYHMNAVVLNSERKWLYMFKIMCSLKEGGF
                     LLIATDTQFRSETGQMISLLVGQAGTSLSTAVLYKSVYKKANTDPLTGAKTRRFLKAF
                     LRKEIERSQRHNKHFCILFFDIDNFKSFNDTYGHNIGDEVLKLIYRITKMTIRKIDLV
                     ARYGGEEFVVVLPETEFKNAFITAERIRKNIEKCPIKDILDVDRNVTVSIGIAEYGVD
                     GFSEEELINKADWGMYQAKSKGKNQVCSFRDYKIEYEQSQSMEDINTEQEDSKL"
     gene            17729..18211
                     /locus_tag="PLG49_04295"
     CDS             17729..18211
                     /locus_tag="PLG49_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOQ16573.1"
                     /translation="MRLKILELLEKNSRLATKDIAIMLGVPQDEVEKEIKEMEKEQVI
                     CGYNTLINWDKTNKDLVTALIEVKVTPQRGEGFDKIAERIYRFDEVKAVYLMSGGFDL
                     TVIVEGKTLKDVALFVSQKLATLDSVLSTSTHFVLKKYKDHGIPFEEIKKDERMVVSP
                     "
     gene            18208..19368
                     /locus_tag="PLG49_04300"
     CDS             18208..19368
                     /locus_tag="PLG49_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ16574.1"
                     /translation="MKNLIATKVSNLPPSGIRKFFDIASQMEDVISLGVGEPDFDTPW
                     HIREEGIYTLEKGKTIYTSNSGLLELRQEINRYLSRRFNISYDPQDQILVTVGGSEAI
                     DLVLRAVLNPGDEVLIPEPCFVAYKPCTILAGGVPVTITTKAENDFRLMPEELEAAIT
                     EKTKVLILPYPNNPTGAIMEKEDLERIVEVLKDRDILVVSDEIYAELTYGREHVSIAS
                     LPGMYEKTLVINGFSKAYSMTGWRMGYAAGPKELIAAMTKIHQYAIMCAPTTSQYAGI
                     EALKNGDTAVAEMRESFDSRRKFVVNGFRSIGMDCFEPLGAFYVFPSIQKSGLTSEEF
                     CSRLLYEEKVAVVPGTAFGDCGEGFIRCSYAYSIDNIKEALRRIEKFMKQFT"
     gene            complement(19418..19840)
                     /locus_tag="PLG49_04305"
     CDS             complement(19418..19840)
                     /locus_tag="PLG49_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOQ16575.1"
                     /translation="MKELAQQLKEKNLKVTPQRLAIFQMLTNTDEHPSAEAIYKALES
                     IHPTMSLATVYKTLDALKKANLIQELNVGEDCFRYDARTHSHPHLICISCTKVVDMES
                     IYLDDIRKRIEQDTNFDIVNEKIYFYGVCPDCKEKKED"
     gene            20060..20536
                     /locus_tag="PLG49_04310"
     CDS             20060..20536
                     /locus_tag="PLG49_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOQ16576.1"
                     /translation="MNVDLINPFVQGTQAILRDVCQETPRLGKVYLKKSPYASGSIVV
                     VIGLTGDIKGQVIFSLDKQAAVTISSKMMMGLEVEELDEMAKSAISELMNMILGHTAT
                     IFYNKGIFVDITPPSLFVGNDIQISISKIQTICIPLHLEDGAMFEIDVAIQENFSQ"
     gene            20548..21054
                     /locus_tag="PLG49_04315"
     CDS             20548..21054
                     /locus_tag="PLG49_04315"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOQ16577.1"
                     /translation="MNIVLLEPEIPQNTGNIARTCAAIGADLHLIKPLGFSIDDKHLK
                     RAGLDYWHLLNVYYYENFEDFKSKHPDGLYFMATTKAQKVYTEVKYTKDCFIIFGKET
                     AGIPESILIDYPETCIRIPMREEARSLNLSNSVAIVAYEAMRQLNFEGLLKEGELRDY
                     KWNEIPGK"
     gene            21590..21868
                     /locus_tag="PLG49_04320"
     CDS             21590..21868
                     /locus_tag="PLG49_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16578.1"
                     /translation="MIEVLCFVFSACGSNKSSAREEKQQVTENTHNEDIMSSDFASNE
                     SFDKSDDNRSTEVELPAQKQFVDRSYKLFYGKWEITEIVGEHSRFGAY"
     gene            23013..24098
                     /locus_tag="PLG49_04325"
     CDS             23013..24098
                     /locus_tag="PLG49_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16579.1"
                     /translation="MEKDPAGYEKCYGYDAAGNKIWETDKEKHVKTNYEYDGFYRLKR
                     VDSPEGIRVEYTYNALDSILTQTIGGSQTTVYTYDGLNRLTSKRDPSGRVESYNSYDN
                     AGNLLSRTDRNGRTTSYEYDSFYRLKSETVDDAYHSQRTYKYDKVGNLIEETSRSISQ
                     YSKVMTYDDLNRLLTKELPGGNKIEYTYEDAEHKEIMTDPAGNKTIYSYDKMNRLEIV
                     TTDERDTVYTYDANGNRKSLTYPSGITALYEYDSRNLLVRLTNYTKMQVKTAESIEDK
                     GEYESLTVTAAVYHYPGKGIEKIYEYQYDGNGMQTCKIEPKGKTEFIYDDAGRIKTVI
                     EPDGKKRLTPMTALETERPKELKETGC"
     gene            24134..24421
                     /locus_tag="PLG49_04330"
     CDS             24134..24421
                     /locus_tag="PLG49_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16580.1"
                     /translation="MVKTIEMNNNMVINTEYGYDNNGNQISVKEFSSSGNKTSTYEYD
                     GFNQMVKARTTEGKNISCNYDALGLRVSKTADGKTTNYYYRYGSVILEKNR"
     gene            24521..25642
                     /locus_tag="PLG49_04335"
     CDS             24521..25642
                     /locus_tag="PLG49_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOQ16581.1"
                     /translation="MQSSGGGIVNEYDYDIFGNPTLENEAEGKENPYRYAGYYWDKET
                     GYYYLRTRYYDPEIARFITEDSFTGYYDDPLSLNLYTYCMNSPINYWDPDGRYYVTDP
                     VTGEKIHASEWESRRNFRAKVRDSQGRGVFVSTGVSAYDWALQNKVNKGTGNGGTYTY
                     KDKDGNTVTKEWTITNDEFLDSNALTQAEITEICRKKNPGLVERGFDKAIYEYAQKKG
                     INPKVILATLAQEQKWCKNGRYDKAFGVGPGGNPSAFAENETGGIASSVNTFLNLFNE
                     GLKKQNNGGLKPMHVNQDYKQNGVWMETDAAARQEYGISGAEWMNRNPKYAEYLKNGI
                     DVQPVNAAMYAKLRYTPWVDFPVQNSHPLQEWHDLFRSF"
     gene            25701..26378
                     /locus_tag="PLG49_04340"
     CDS             25701..26378
                     /locus_tag="PLG49_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16582.1"
                     /translation="MKRKINILLIVFTIALVFCSCSIKQNSSEGSLNNNANSIESQKK
                     TSGSLQDLDSNKTAINPEASPSNQTIKPSNNSVPGLKEDDFGVVFSNALVKVTDNPDK
                     MIELWGRGKVKNDDEAFIGTDPSAKYRRYEYYYIKENSGIKAHVRRGEIDYIYFMDIT
                     DWYTNRGIKKGDSYNKMVEAYGKPTKESIEDSELVCEYPLKNVSLMFKFDKDKNISNI
                     TIWNYLN"
     gene            26691..26858
                     /locus_tag="PLG49_04345"
     CDS             26691..26858
                     /locus_tag="PLG49_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16583.1"
                     /translation="MGCTFLQKNKAVQQHLQASSSVGHAEYANKAPCRGLRKRADLGV
                     HRQGNAGIHVQ"
     gene            26836..27180
                     /locus_tag="PLG49_04350"
     CDS             26836..27180
                     /locus_tag="PLG49_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16584.1"
                     /translation="MPEFMFNERGPITGGELFKTLSEMAVKDEIRERVRGIEEHIRTG
                     FPVPKDEMDFYEAHKPNPVKVDPQEEERNRQLFEKYGEPVSIAFWADETSMEEALEML
                     EQEEKKKGRGKK"
     gene            27432..28367
                     /locus_tag="PLG49_04355"
     CDS             27432..28367
                     /locus_tag="PLG49_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16585.1"
                     /translation="MNQYENIPKELKQLPQWVCHRNKVPFNPATGKAAKAGQPDTWAR
                     FEDAVKASGSFGGIGFEFNNNGIVGIDLDKVIADDGTVSDEADGIVAMLDSYTEYSPS
                     GKGLHIFVKGDISVDGRKKGFIEMHKAKRYFTMTGNVYGNKKPINEYTEQVKQLFGKY
                     FSDSKSGKSVGTNNPCISSAKDYLSIGLAKDAVFKALWDGEYQSEKCTSESEADLALM
                     GKLLYWCSGNIDAAIEAFIKSPYVAGKDDKYTTKLERSDYLQRTAAKAMQGLTSTAAG
                     DDEQYCKQQEFTLDDMGNARRLSSYKYRSYISQKC"
BASE COUNT        10194 a         3901 c         5785 g         8500 t
ORIGIN      
        1 aaattacatt ttatttaata acttttaaaa aagtattaat tataattaaa ttaattataa
       61 cttgtgtttt ttatatgatt tttttgatat aattaaccat tgaaatatga tataggaaat
      121 tgtgtataat tttaaaaaaa tctagatttg ttgtttattt ttttatgctt aaaaggtggc
      181 gctatgacga caaaagaggt gacagaaaag tttcttaaga tcagggatca gttgattcaa
      241 agagacttta aaaatttaaa tgaaagtcaa agacaggctg tattaaaggg taagggtcca
      301 caaatggtta ttgcaggtcc tgggtcagga aaaactcacg tgattatgca ccggctttta
      361 tatttaatga cttacgggcc agtttatcat acaaatttaa ttcctcaggg gataacaaaa
      421 aatgatttag aaatcattaa agaaaagcct gagtctgctc aggcaagatc tcttttatat
      481 tcttttgcag tcaggccaga aaatattttg gtcattagct ttacaaaagc ggcagcagat
      541 gaaatgaaaa caagattttc ctttttagcc agccagcata agctggatta tggaaaagta
      601 attttttcga cttttcatgc tttgtttttt agaatcttaa gatctgttta tggatattct
      661 ttaaaagaaa tcctggagga aaatgaaaaa agaataattt tcaggcagat tgcagaagca
      721 ttaaaaattg aatttgagga tgaagaagaa tttatatccg agttacaaaa tgaaataagt
      781 ctgatcaaaa atgatttgat agacatatct tactataatt ctatgatatt gccttcagaa
      841 cagtttaaaa acttctattt aacctatgat agttataaaa gaaaaaataa taaaattgat
      901 tttgatgata tgctttatga ctgctatgat ttattgaaaa caaataataa agtcctcgat
      961 ttttggagaa ataaatttaa atatatttta atagatgaat ttcaggatat cagtaaggta
     1021 cagtatgaac tgattaagct tttggctgct cctgataata atctgtttat tgtaggcgat
     1081 gacgatcaaa gtatttataa attcagaggt gccagacctg aattcttgct aaactttcct
     1141 gaagatttta aaaatgccgg aaaagtcatc ttaaatatta attaccgttc tacaaaaaag
     1201 attgttttag caagtaacaa agttatttca aaaaataaaa aaaggttcga aaagaaaata
     1261 actggtatcc atgatgaagg agcagacatt gtattgattc aatcagaaga tatagaaaat
     1321 gaagccctgc atattgtaga atggattcaa agtttaacaa gaaaagaact ggcttataaa
     1381 gatattgctg ttattttcag agccaatctg caggcaaggg ctttggtaga tataatgttt
     1441 gatttaaata ttcccttctg ccttaaagat gaaataagga atatttatga gcattggatt
     1501 gctaaagatt tgatggcata tttaagatta tcgctggatg attatgataa tgaaagtatc
     1561 gaaagaatat taaataaacc caaaagatat atcagcaaag gtctcatttt tgaagctaaa
     1621 aagaaaaaag gcaacttatt agacagctta tattatcttc ctggtataag aggctggcaa
     1681 ttggaaaaat tagatgaact aaaatttcat ttatcccgtc taaagaaaaa agcacctgaa
     1741 gaagccattg cttatataag aaagaatatt ggttatgatg attatatttt ggagtatgcc
     1801 agttataaaa acatcggcac aaggggcctg atggaagttc ttgatgaaat tatggaaggt
     1861 gcaaaaagtt ttaaaaactg cacagactgg ctaaggcata taggaaagtt ggaagaagaa
     1921 ataaaaaaag ggaaaaagag aggagtaggg caaaatgatg gtgttgtact gtctaccatg
     1981 catggggcaa aggggctgga gtttgaagca gtatgtatag ctggtgctgt agatggaatt
     2041 attcctcatt ataagagtca aggaattagc ggactggaag aagaaagaag attattttat
     2101 gttgctatga caagggcaaa aaaatattta gccctttcta cagtaaaaaa gcgttatgag
     2161 gaagaagtta agccttcatg ctttattgaa gatttaaaag aagatctgaa agcgaaataa
     2221 ataaaagtaa atggatataa aaaacagaat atatttttat gatggatgat taaggaggga
     2281 ctttatgctg tacagcaaaa aacggagggg gaaacaaaaa agtataagta tcattcttgt
     2341 tgtgctatta attttagcga ttatattaag ttctattctt cctgttattg tatatgggga
     2401 agaaaaagac aatcttaaaa taaaaggcga agtaggtttt aaaaataaat atatcatcaa
     2461 tatgaacacg cctttgattt tggaaataac aaatgagggc aaggagttta atggtacggt
     2521 tcaagttaaa gtgcttacgg gtgatcatga gattaaagaa tatgtgattt atgaacaaaa
     2581 cattacgctt ccacaaggat caaccaaaaa agtgacaatg aacatcaaac ccgtcagctt
     2641 acaaaaatat tttacggtga gtttggtttc agattcaaaa tcaaaagaag tcgccaaaaa
     2701 gataatctat gcaacacctt ttgaacctta cagaggtttt gttggcgttt tatcagatga
     2761 ttttgaaagt gtaagctact taaaaaatct gaaactggat actgcacgca gtgcgtttat
     2821 tggaaacaga ctggttgaat tgactgaaga aacatttccg gaaaatctgg atttattcaa
     2881 taactttgaa atgattatta ttaataactt tgatacttct aaattaagtg atgagcaact
     2941 ggataatctg aagacatggg tgttatccgg cggagtcatg gttcttggga caggcgttaa
     3001 tgcatcgaaa gttctgagag gttttgataa agactttata aatgtaaaag ataatggcat
     3061 gattagcatt aatgactttg cacaaatgga agaaataggt ctccacgcct tagaagtgta
     3121 tgcaatggat gtagcggatt tagagattga aaatggaact ggtattttat ttgcaggcaa
     3181 tctagccatt agttctttaa tcaatagaag tgaaggcagt attgtggttc atcattttga
     3241 tttaggctta aatcctatag ctggctggca gggtaattat tatatgctta cggaattgta
     3301 tatgaatgtt cctgatgtga ttgaatttgc ttctcaagat agagatagtt attatagtta
     3361 taattatgac tattataatt atagcttgag attatttcca agaccaaacc aaaattactt
     3421 atttgttatc gcagggatta ttattgttat ttatattttt cttataggtc caatacttta
     3481 tatcatttta aaaattaaag ataaaaggga atatggctgg tttatcatac cagttgcttc
     3541 cgttgttttt tcgttgctta ttattttaat cagcagggct tccattttac ggcatcctct
     3601 ggcaagcagt gttggagtaa tttctttagg agaaggcgaa accaggggaa caatggaagt
     3661 gagtatggga gtttttacac ctaaaagcgg agaaaatgtg atagaaatct taaaaggcat
     3721 taagcctcaa ataggcagtt tttactatta ttataattcg tataatacag attcaaagaa
     3781 agaaaaaatc ataggtaagg tcaatattgg ggaacaagac aagattactt tttacaatac
     3841 caatagctgg gccatgaata aaattacagg caaaacaaaa tttgatgctt caggtcctgt
     3901 taatgcaacc atttatttta aagacagtga actggttgga gtcattgaaa ataatcttgg
     3961 ctttgattta gagtattgta ttcttgtatt tggtaataat tatcgttata tagggaagat
     4021 agaaaataat aaatcagtta atattgatgt agctttaaaa tataatacca gtaataaata
     4081 tgatgttttg aatcaaatat ttggcataac ttttgataaa aaggatttaa aagaaaagtg
     4141 gggagatatc ccagaggaag aactacaaga aattatacaa agaagacata tttacgaaga
     4201 atcttttgat agatattata actataatac gatgcaatat tacggccaga attcttccag
     4261 ctatgattat tttaaagata attatgttgt tctttacgga tttaatgatg aggagttatt
     4321 agaaggaata aaggtaaaca atcatttggt ggagcattat aaacaaaact tatttgttat
     4381 cccattaaaa attgattttg aaaatatgga ttatattgaa attccatatg gttttataaa
     4441 accatttatc acaagtgaag caggatttag ttatgacaac tatgatgatt ccgtatggat
     4501 taccagtgat ggcgctgttg atataacttt ccctgtgcct tctgatttgg atgttaagga
     4561 attccaggtt cagtattcag aaaccatcaa tgtagaaaaa gctttaatat ataataatgt
     4621 tacagatgaa tgggaagaat taagtgcttt accttatagc gataatgctg aggattatat
     4681 tagtgacgaa agcatgatac aattaaaatt tgaaatacaa ttagtaaatg acgttccaga
     4741 tgccagactt ccggttcctt atatttactt gaaaggagat aaataaaatg ctggaaatta
     4801 aaaatttata taaaagatat ggcaagtttc ttgcagtaaa taatttatct ctttctattg
     4861 cagaaggcga gatctttgga tttgtgggac ctaacggggc tggtaaaaca acaactatga
     4921 aaatcgtagc aggtttatta aaagcaagtg gtggtcaggt tcttattaat ggaataaatg
     4981 ttatatccca tcctggtaaa cttagggaga agataggtta tatgcctgac ttctttggtg
     5041 tttatgataa cttaaaagtg gatgaatata tggatttcta tgcaggtatg tataaaatcc
     5101 cttataaaga cagaccagca attattgata atttattgga gattgttgat ttgtctcata
     5161 aaagggatgc ctatgtagat agtttatcca gagggatgaa acaaagactt tgtcttgcgc
     5221 gaagtttgat tcatgatccg gatatcctta ttctggatga accagcttct ggtcttgacc
     5281 caagggccag aatagaaatg aaagaaattc taaaacagct tagaacttta ggaaaaacaa
     5341 ttattattag ttcgcacatt cttcctgaat tagcagagct ttgtacttcg attgggatta
     5401 ttgaaaatgg tcagatggtg gcttgcggta aagtcaatga aatcatgcag caactcaccc
     5461 ataaaaggat tattaaaata agattactgg ataaaatgga ggagcttata cgaattctgc
     5521 aagaacagcc agatattcat aacatttttg aaaaaacaga tgaagtggaa tttgaatttg
     5581 atggtgatga tatacgactt tcacagttgc ttaagcaaat catggaaaag gatgtcctgc
     5641 ttactgcttt ccatgaaaaa gaaggtaatt tagaagacat ctttatgaaa atcacaaaag
     5701 gaggggagta atatgatgat aaatcccgta ttgcgccggg agttaaaaac aaaattaaga
     5761 agttggaaag cccctgtttt attaatggtt tatctgatta tattaacggg atttggcggt
     5821 ttagtattgt tattaacctt gtatacttcc tatgataata gttttaaccc tcaggataca
     5881 ttaacggtat atggtctttt agcaggctgt cagatgggtt taatcatctt attagttcct
     5941 gccttaacgg ctggaaccat aagcggggaa agggaaagac agactttgga tttactaaag
     6001 gttacaaaaa tctcatcttt ttcgattata atgggcaagc ttttagcttc tatcagtcag
     6061 attatgctga tggtgcttgc ttccattcct gtttttagca ttatctttat ttttggaggc
     6121 gtatcattaa agaatattgc attattgttt ttatattata ttgttatatc cattaccttg
     6181 ggaagtgtag gaatattttg ttcgacttac tttaggaaaa caaccgttgc aacagtagtt
     6241 acgtatttgt ttattctgat tttctgcttt gggagtttga ttgtatatgc tattgtacaa
     6301 gaatttgttt attatatttt ttcctattca gatggtctaa cctattttca atcggtatta
     6361 ttgattggag gaaatccatt tttaggcttc ttatctgtta ttgagaatca gttaggcacg
     6421 acggatacac tgtattattt tttgaatgcc cataataatc ctctgaatat taatattaaa
     6481 ttatggcaat ttaatttggt atttaattgc ttagtaagtg tactgatgat atttttaagt
     6541 gttcttaaaa tacgccgtta ggcaatgaaa aggtgatata tttgaaagaa ttatacaggg
     6601 ctcttaaacc tttaaaaaga aagttgattt tagaaaaatg gctgcattta atcttactaa
     6661 gtaccattat tgctatggga ttgaatattg ggcttcttat tttatctaaa tttacgttga
     6721 tttcttatat ttatttaaaa tttataggga ttataattct ttctctgttt acaggctctt
     6781 taacgcttct tttttgtctg cctgatatga ggaagctggt aaataaaggg gattctttag
     6841 gctataaaga acgctttgtg acggcttttg aaatacttag tcataaggga aaacaattaa
     6901 attcctttga aaaaatggtg gttgaagatg ccattgaaaa agccagaaaa gctaatttta
     6961 aaagagaata tcatatatct ttgcctgaaa agaagttgaa agttttggct gtcatgctga
     7021 ttggggcaat gattgttggt tttgctccaa gtccggtaaa agaaaagctg gagtatcaga
     7081 tgcggcttaa agaagtgatt gaagaaaaga caaaggaaat tgaaaaggta caaaatgaat
     7141 taaaagagaa taaagaatta acagaggcac aagtaaaaca aattaataaa gaattagatg
     7201 aattaaagaa aaacattaag caagcaaaga atgaagcaga cattgtaaga gctcgtcaaa
     7261 aaacccagga ggagctaaag agaatatcta aagattccgt tcaaagagat ttgaaaaaaa
     7321 taggagagag tttgtctcag catgaattaa caagagaatt aggggaccaa ttacaaaaag
     7381 gaaatataga agatattaaa agaagtcttg aggaactaag gcaagaactt gctaatatgg
     7441 accagaatca gttaaaggaa ctggctaatg cttttaaaga agcggctgaa agtcttgaaa
     7501 gtgattctgc cttaaggcag gccctttcag attatcagga agccattacc aatgggaatt
     7561 tatctgatct ccaggaatgc tatcagcagc tatctgagca gttggcccag agggcttctg
     7621 aaaatgaaga cttaagaaaa gcgatagaga agattaatga agcaatgaat aaagacaata
     7681 atttatccca aaatcaagga caaaaccaga gccaaggtca aaatcaaaac caaagtcaga
     7741 atcaaaacca gggtcaaaat caaggccagg gtcagggccg gggccaaggt caaagtcaag
     7801 gacaaggcca ggaccagggc aagggtcagg ggcaaggccg gggaagaggt catgttccca
     7861 atgaaaatat ttattccaga caagcagagg ataaagagga ctttgatgct tatgtagaag
     7921 gacaaaaaaa tcaaggtgga caattccatc agaaagaaga aaagatgatc ggaaaaaaag
     7981 gcgagtcggt tccttatcag gaagtttttc aggaatacaa aaaagaagct ttaaatcttt
     8041 tggaagatga taatatccct tatggaatta aaaatctggt gaaagagtat tttacatctt
     8101 tagaataaga tccaaaaata aaacagaaaa ctgtcaggtt tgctttatta taactgacag
     8161 ttttcttcat atcatctcga ctaaatggaa ggactttggt aaaatactta tattacacaa
     8221 tatagaggtg taaatatgaa attttataat ccttgggctt ttttaggcct tgtatcaatt
     8281 cctattatca tagtaatgta catattaaaa caaaaatatg aagaaaaaaa agttccgagc
     8341 cttttcttat gggaaaaggc cttaagctat tctgaagctt atatgccctg gcaaaagctc
     8401 agaaaaaata tattattatt tcttcagcta atgcttgccg gattactggt tattatcctg
     8461 gcaaagccat atatagaagg cgggatgata aaatcagaaa gctatatttt aatactggac
     8521 cagtctgtca cgatgcaggc aaaagatgaa agcccaaacc gatttgagga agctaaaaat
     8581 aaaatgtatc agtttatcaa taatttggaa ccagcggcta aaatatcggt tgtggtgatg
     8641 gggaaagaac cttttatttt agccaatcag tcaacggata aaaatgctgt gattaaaaag
     8701 attgaagatg taaaagtgtt aaacagcggg ataaatttag agaatacaga gactttagtt
     8761 aaggcgatta atgacaatat caaaagcagt atatatattt tttctgataa ggatattaaa
     8821 atgacggatt taaattctaa tttaataaaa gttggaaaac attatgagaa ttgcgggatt
     8881 acattattat cccatgccaa agaggaagaa caaatcgcag ctttagttaa agtaaagaac
     8941 tttggaaatg aaaacaatga gctttcggtt gccctttatg gtgatgaaaa gcttattgac
     9001 ataaagaggg ttgaattggc agcaaaagaa gaaaagaatg ttttttttac cgggatatct
     9061 aaagagatta aggttttaaa agctcaaatt gatttagaag atatccttga agtggataat
     9121 gtgatgtatg atgtggtata tgaagatgta aaggaaaagg ttctgctttt aaccaggcaa
     9181 aatatctttc ttgagcagat tctttccctg ctgccacagg tcgaattata taaaggcgat
     9241 ttagacagca tggataattt acaaggctat tatctttata tatttgatgg ctatatgcct
     9301 gaaaagttac cagaagacgg gcatatcctt gtgtttgatc ctcctttgga aaatccttta
     9361 ctggaggtaa aacaagatat tgaagtcagt acactttatc cttcaggagg agaacttttg
     9421 gcatttttag atcaaattaa atttgatatt gccaaaacaa aaaaattatc tgtgccccaa
     9481 tggggacaag taatattaaa ttctgaagaa acgcctctta ttattgctgg tgaacgaggc
     9541 gaacaaaaga taatggtttg tggttttgat ttgcatcaaa ccgatttgcc tttaagaacg
     9601 gaattcccga tttttattta taatacgata cagtggttta ttcctgaaaa aattcacaat
     9661 ttggaaaaga ttattgcagg agatacgata gagtttcaaa ttgcaccaga atctaaggaa
     9721 gtcagtcttg taaaacctga cgggacttat gaaaggctgg cacctccttt tccacttcag
     9781 ccttataaaa ataccaatga aacaggcata tataccctgg aacaaaaagg agaaaaaata
     9841 gacagttttc actattttgc tgttaatcct ttagttgaat gggattttgc aggcagtttt
     9901 gcggaaagca atggagaaga tgcaaaagag aattctcaaa cttatgacaa tatgatagaa
     9961 ggaaatagaa atttaaaaaa tattttattg ataatgcttt tagtattgat gtttttagaa
    10021 tggtgggtgt atgtccgtgg tatttaattt tattaatact cgctatttac tgttaattcc
    10081 actttttatc ttttttctat attttacatc taaaaagcta actttatctt cttataaaaa
    10141 gtctttttta ctttgtctaa gatctattat tgtaatcctg ttaatttttt ctttatcggg
    10201 actacaggtt aaaaaaatag tcgataagac aacgactatt tttgcagtag atttatcgga
    10261 cagtgccaag tctgctataa aggatgcaga agcctttata aaaggagcaa tggcttttaa
    10321 aggcagtcat gaccaagtag gtatcgttgc tttcggaagt aatgccgggg ttgaagccag
    10381 cccaagccaa gagcctgttt ttacaggctt ttcttctata cttaacagca attccacgaa
    10441 tatcgcagaa gggcttcaat tggcttctac tttaattcct gaagagtcta aaaaacgtat
    10501 tgtccttatt tctgatggaa aagaaaatat agcggacgct ctgagtcagg ccaaattact
    10561 agccagccag aatattacag tagatgtttt gcctatgaca aatcaaattg aagaagaggt
    10621 acagttaacg gagttaaatg ttccaaaata cttaagtaaa aatcagcaat ttactgttca
    10681 agtaaccgta gacagcttaa tcgatacaaa aggggtactt aaaatataca atgggaaaaa
    10741 tttagtggaa agccaggagg tacaaatccg caaggggcaa aaccgttatg cattttctga
    10801 tttggccagt gatggcggaa gcagaattta cagagcagaa atagaagctg ttgtggatac
    10861 acttttacaa aacaatgtcg cttatgctta tacttatgtt gaagatattc ctcatatttt
    10921 attaatcgag caggaaaata gcggggccga gataaaggag atattaaaaa attctgagtt
    10981 agtaatcgat gtggtcaatc cttttaccat cccgcaggaa gcagatttac ttgctcaata
    11041 tgatggaatt attatggcca atatatctgc ggatgattta acagaaaaat ttttaaacaa
    11101 tctggaaaac tatgtaaaac atacaggtgg cggattaatc gtatcgggtg gagaaaatgc
    11161 ttatgcttta ggcaattatt ttgatacgcc tctggagaca attctgcctg ttgatatgga
    11221 attaaaagat aaaacgaaaa tcccggatat ggggattgta cttgttacag accggtctgg
    11281 cagtatgatg gaagcacctt atggggtttc aaaattagaa ttggcaaaag aagcagcaat
    11341 tcgttctacg gacgttttaa atgaatttga taaaatagcg gtgattgctt ttgacgatca
    11401 accacaggtc attgttgaat tacaagaagt aaaaggaaat ctgggggcta tacaggataa
    11461 gattgcaggt atttcaattg gtggcggtac aagcattatc cctgcccttc aaaaggcata
    11521 tgatattctc tcggatgcag atacgaaagt caagcatatt attttattaa cagacggtca
    11581 ggctgaaacc agtggttatg acgatttgat aagtcaaatg aaattaaaag ggattaccct
    11641 atccactgta gctgtaggcc gttattcgga tgtagtgctt ttagaaagat tagcccgtga
    11701 aggtggcggg agatattatt atactgatga attttctgat ttaccaaaaa ttttcatgaa
    11761 agaaacgatg acagccagta agacttatat tcaaaatgaa actttttacc ctaaactgac
    11821 aagactttct ccgattgttg aaggcattga caccctgcct cctttacacg gatatattgc
    11881 aacaagtcct aaaagccgtg cagaagttat tttcagcagt gaaaaagatg acccggtttt
    11941 agccgtatgg cgatatggct taggaagaac ggctgcatgg acttctgata tgcacaatca
    12001 gtggagtttg gactggctgg cttcttcttc aggggtagaa gtttttagaa atttagtatc
    12061 ctggattatc agaaagccga tgagtgacag tgtaatggtt gaaggaaagg tggaagggga
    12121 aaatataaga ttgaaagctt caattcccaa tatgggatca gaggcggaaa ttaaggttaa
    12181 tgttgttgcg cctgatatga cagagcatga gatttattta aaagcttccg caccagggga
    12241 atatacaggg cttttcccag ggaataatat aggagcttat atccttaatt ttcaagtcaa
    12301 tcaaaatgga gaagaagaaa gtattcatac tggaataaat gttccttatt cgcctgaata
    12361 tgatattaat aaaattacac aagatacagg cttactcaat caaattgccc atataacggg
    12421 tggaagagta ttaacgcaac cagaagaagt ttttaaagaa atagaatcta cgatatatgg
    12481 agaaagagat attgatgact ttttgttaat ccttgccttg ttattcattc tttttgatat
    12541 tgccttccgc aggataggag ttatcaataa taaagttgaa gaaataatca tttcggtgat
    12601 tcagttcttt aaaaataaga agaaaaagga gaaaaaagta ctgattgtaa aagaaaaaaa
    12661 taaagctcca gaaaagacga gtgttccaaa agtaaaaaaa gaaaagaaaa aagaagataa
    12721 gaaagaaaaa gaagaagtta aaaagattga aaatactaca tctgctttat tagctcaaaa
    12781 aagaaaaagg acaagttata aaaaataaca aactatatgg ttgaattgcc gtatatattt
    12841 aataatagaa gatcaaatga gctatgagaa gcaaaggaga atgatatatg gtagaagaaa
    12901 aaatgaaaat tttaaagatg ttagaagaag gaaaaattac agcagatgaa gccgttaact
    12961 tgttaaattc tttagatggt aaaagtaaac ccaatctgga caggggatat gaaagtaaaa
    13021 acatgcagaa taatacccag ccaaaaaata aaacagaatc ttcttttacc atggaccttg
    13081 aaaagaaaat tgaagttttg tcaagagatt tagaacccaa aataaaaaag gctgcaaaaa
    13141 cgattatgga tacggcggat tcttttgcag aaaaaatagc aaaatccttt tcttattctc
    13201 ctgctggtcc tcagtcaaac gatatgactt ttgaaatgta tgtgtcaaaa ggcgataata
    13261 cgcagttaag actaaagagt aagaatgggc caattcatat aaaaggctat aatggagata
    13321 aaattacggc aaaaattaag tatagctgta aaggcggcag tcaaaaaatt gaacttctta
    13381 aagttgacaa tacttattat ttaaattatg atgaagcaaa ctttacattt gttgcaatag
    13441 aagcttttgt tccggagttt ttatttagca gagtattttt tgagagcaat agtaatgtat
    13501 atgtggatgc cttatctgga gacgagataa cgattttaac caatgacggt tccattgagc
    13561 taaaaaatat atcagcgaag aatatgaagt tggaaagcgc aagaggaaat atcttattag
    13621 aaaatataac tggagaatta ctccgggcag aaagctgtaa tggctatatt gatgtaaaat
    13681 taagtgatat tgcaaaaatg gatttattga cttctaatgg agaaattaat attgattcat
    13741 ccatcagaga gctaaaagcc gctaattatt atgaatggaa tgccgaaact tccaatgcag
    13801 ccattaaact aaacagcttc agaggttttg atattgggta tgactttaaa gcccttacat
    13861 cattaaatgg cattcatgtt catataccta gtgtggattt tatagaaaac gaaaagaatt
    13921 ttgtgaaagg aatgactaaa aattatcctt ctgcatcaaa aaaagtaaag ataaatttgg
    13981 agacttctaa tgctccaatt acgctctatt aaaagactcc cgtaagggag ttttttaaaa
    14041 tagagattat tgtaaaacct ataaaaatat tatataataa attaagacaa tttatacata
    14101 attcaataat aaggcgcagc cttttttctt atttataaga aaaagaacca gtaaattgaa
    14161 caattgtctt aaaaaaatgt gtatttcatc ggagtgagta aaatgtcaag cttagacgat
    14221 aaactatacg aaaagactgt agaatgtcct gtatgcggga atgtatttaa agtgacagca
    14281 gtcaagagaa aagcttatgt cgttgaaagc cgggatactg atttttgtgt taaatataaa
    14341 gatattaatc ctttatttta tgatgtgtgg atttgccagt tatgcggata tgctgctcaa
    14401 aagagtactt tttctaagat tacatataaa aggtctcaat taataaaaaa acatataacc
    14461 cccagatggg ttgctaaaaa agaaccagca gtgattgagt atgaagctgc gattaataaa
    14521 tataaattgg ctttaatcaa tgcacaaatc agtaaggcaa aggatagtga aatagcaggt
    14581 atttgtttaa aaattgcctg gctttatcgt tttatgggta agaaagaaaa tgagttgcat
    14641 tttcttaatc atgcgttaaa taaatatgta gatgcttttt caaaggaaaa gtttccggtt
    14701 gaaaacatgg acgaagctac agtaatgtac ttaatagcag aactttatag aagagttgga
    14761 aattttgaag agtcggccag atggttcaat aaagtgatta gtcaaagagg aattccggaa
    14821 agaattctaa atttagcaag agagcagtgg agtttattaa gggaagattt aaaaaatgaa
    14881 aaaagcaaaa gccaaaagaa taatgatgaa gtatccgaag ctttaaaata aaaattttaa
    14941 aatttttaca ttttttattt tttattttga acaattgtaa tataataata taattaatta
    15001 aaacttgctt taggagagga cataacatac aatgaaagtt ggaaaaattc cccctaatct
    15061 tttaaaggag tatgttttta gtaatttaaa aaatcataaa aaagaaatta ttttaggtcc
    15121 aggaattgga gaagatagtt ctgcaatcca gttggcagaa gatgagattt ttcttgtttc
    15181 tacggatcct ataacaggag ccgataaaga tatgggttat cttgccgttc atgtttcatg
    15241 caatgatatt gcttcttgtg gaggagagcc cattggtgta ttgttgacaa tattgcttcc
    15301 agaagcttca gataaagaag atttgataag aataatggag gatgccaata gagcttcaaa
    15361 ggaaatagat gttcagatta taggaggtca tactgaagta accaattcag taaatcgtcc
    15421 tgtcatatct gcaacggcta ttggaaaggt gaaaaaagat aagttggttc gaacagaaaa
    15481 cgcaaaagtc gggcaggatt taataatgac aaaatgggca ggcctggaag gcacagcaat
    15541 cattgcaaaa gattgcaggg aagagttgct gaaggtcttt tctgaagatt ttttaaatag
    15601 cgctcaaaat tttgctaatt atttatctgt cgtttctgag gggaaaattg cagcagattt
    15661 tggagcaaca tccatgcatg atgctacgga aggtggcgtc ttaggggctg tatgggaaat
    15721 agcagagtgc tcaggcgtag gcgttgaagt atatctggat aagataccta taaaaaagga
    15781 gacaaaagat atctgcaatt attacaatat ttctccttat cagttaattt caagtggttg
    15841 catgattata acgacttatg atggaaaccg tcttgttgaa caattaaaaa tgagcgggat
    15901 tgaagcacat attattggaa aaattactga aaaagacaga atttgcattc aaaacaatga
    15961 agttttgccc ttaagacagc cggatgtgga tgaattatac aaagtaataa aataagccta
    16021 catggaaaat gggggaggac cttatgaatt taaaagaaag gaagcgaata gagaatatac
    16081 taacccgttt caggttagtc gctttcctat ttgtgttttt tatggttcct ttaaatatca
    16141 gcatgagcag gcaaatggaa gcactattat taaatagaat gtttgttttt ataacatact
    16201 atttaatttc tgtaggcatt ggttttttac tgtttaaaaa tataaagaat gaaaaaatgt
    16261 ttgatttagc cattggtatt tctacagttt ttgattttat tgctatccct tgggcagcta
    16321 cagtaatctt gccagtttgt gaccttccaa ttgaagttgt ttatattatt gtgcaaatga
    16381 cgatgttgat tagataccgt acgaattttt attctctgat aggcaatgcg cttgtgttgc
    16441 ttaatatatg gttgtatgaa ttaactattg gagcaagttt ttactggcgc ttttggagta
    16501 taagaggttt attttggacg attatatttg gctttaatct gattattttt tggaaactgc
    16561 ttaagaacta tgacgaattg ctggagcaaa aagaattaga gaatatcacc atcgaagatt
    16621 ccaagcgaag tattgataat ttgattcata ttaacaaaat tagtgcctcc ctgaatgaat
    16681 ctttgtctca aaatgaaatt attaaaatat tacttagcaa tgtatctaaa atggttaata
    16741 gcgaagactg tgccgtttta ctttattcca aaaaggaaac tgacggctat ttatacaatt
    16801 atagaaaatt taaagaatcc agtagtaata acagagaaat tattaaatat gaaattatta
    16861 atattggtga tgaaattaaa aaaatcaaaa gtatgaaaga ttataagcat gtaataggag
    16921 ctcgtcagcc tttttacatg gctgatttgg tcaatatata tcatatgaat gcagtcgttt
    16981 taaattccga aagaaaatgg ctctatatgt ttaaaattat gtgcagttta aaagaaggag
    17041 gctttttgct gattgccacg gatacgcaat tccgttcaga aactgggcaa atgatttcat
    17101 tattggtagg ccaggcaggg acatctttgt ctactgctgt tttatataaa tcggtatata
    17161 agaaggccaa taccgatccg ttgacaggtg caaaaacaag aagattttta aaagcctttc
    17221 ttagaaaaga aatcgaaaga tctcaaagac ataataaaca tttttgtata ttattctttg
    17281 atatagataa tttcaaatca tttaatgata cctatgggca taatataggt gatgaagtac
    17341 taaaacttat ttacaggatt accaaaatga ctatccgtaa aattgattta gtcgccagat
    17401 acgggggaga agagtttgtt gtagttctcc ctgaaacaga gtttaaaaat gcattcataa
    17461 cagcagaaag aataagaaaa aatatagaaa aatgcccaat caaagacata ctcgatgtag
    17521 acagaaatgt aacggttagt atagggatag cagagtatgg tgtggatggc ttttctgaag
    17581 aagaactgat taataaggcc gattggggca tgtatcaggc aaaatctaag ggtaaaaatc
    17641 aagtatgttc ctttagggac tataaaatag aatatgagca atctcaatct atggaagaca
    17701 ttaatactga acaggaggat agcaaactat gagacttaaa attttagaat tgttagaaaa
    17761 gaacagccgt ctggcaacaa aagatattgc cattatgcta ggcgtgccac aggatgaagt
    17821 ggaaaaagaa attaaagaaa tggaaaaaga acaagtgatt tgtggttata atactttgat
    17881 taactgggat aaaaccaata aagatttagt gaccgcctta attgaagtaa aagtaacgcc
    17941 tcaaagaggt gagggttttg ataaaattgc tgaaagaatt tatcgttttg atgaagtaaa
    18001 agcagtgtat ctaatgtcag gtggttttga tttaactgtt attgtcgaag gtaagacttt
    18061 aaaggatgtg gctttatttg tttcacaaaa acttgctacc cttgattctg ttttaagtac
    18121 cagcacccat ttcgtactta aaaaatataa agatcatggg attcctttcg aagagataaa
    18181 gaaagatgaa aggatggttg tttccccatg aaaaatttaa tagcgacgaa ggtaagtaat
    18241 cttccacctt caggaatacg taaatttttt gacattgcta gtcaaatgga agatgttatt
    18301 tctttaggcg taggggaacc tgattttgat acaccctggc atatacgtga agaaggtatc
    18361 tacactttag aaaaaggaaa aaccatatat acttccaatt caggtcttct ggaattgcgc
    18421 caggaaatca ataggtatct gtcaagacgc tttaatatat cctatgatcc tcaagatcaa
    18481 atcctcgtga ctgtcggggg cagtgaagcc attgatttag tgttaagggc agtattaaac
    18541 cctggagatg aagtattaat tcctgagcct tgttttgttg cttataaacc ttgtacgata
    18601 ctggctggcg gtgtacccgt taccattacg acaaaggcag aaaatgattt ccggttaatg
    18661 ccagaagaat tagaagcagc aatcactgag aaaacaaaag ttttgattct tccttatccc
    18721 aataacccaa caggggctat tatggaaaaa gaggatttgg aaagaatcgt agaagtactg
    18781 aaggacagag atatcctggt cgtttcagat gaaatctatg ctgagctgac ctatggcaga
    18841 gaacatgttt ccattgcatc cctgccaggt atgtatgaaa aaactttagt gattaatgga
    18901 ttttcaaaag cttattcgat gactggctgg agaatgggtt atgccgcagg accaaaagaa
    18961 ttaattgcgg caatgacaaa aatacaccaa tatgcaatta tgtgtgcccc gacaacaagt
    19021 caatatgcag gcattgaagc attaaaaaat ggggacactg ccgttgcaga aatgagagaa
    19081 tcctttgata gcagaaggaa atttgtggtg aatggcttta gaagtattgg aatggattgc
    19141 tttgaacctt taggtgcctt ttatgttttc ccttctattc aaaagtcagg tctgacttca
    19201 gaagaatttt gcagcagact tttatacgaa gaaaaggttg ctgtagttcc gggaacggct
    19261 tttggcgact gtggtgaagg ctttatcaga tgttcttatg cttattctat agacaatatt
    19321 aaagaagccc taagacgaat tgaaaagttt atgaaacagt ttacataaaa atcaaaaaga
    19381 cggtatttta attaaaaatc aaaataccgt cctttcttta atcttccttc ttttctttac
    19441 agtctggaca aacaccgtaa aaatatattt tttcatttac gatatcaaaa ttcgtgtctt
    19501 gttcaattct tttacggata tcgtctaaat aaattgactc catatccaca acttttgtac
    19561 aagatatgca gattaaatga gggtgcgaat gagttctggc atcgtatctg aagcaatctt
    19621 ccccaacgtt taattcttga ataagattag ctttttttaa agcatccagg gtcttataga
    19681 ctgttgccag gctcattgtc ggatgaatag attctaaagc cttataaatt gcttctgcgc
    19741 tgggatgctc gtcggtgttg gtcagcattt gaaaaatggc cagtctttgt ggtgttacct
    19801 tcagattctt ttcttttaat tgctgagcga gttctttcat gggaaaaacc accttactga
    19861 taataatttt tatataataa taatattaga tatttgaatt ttcgtcaagt gacaaaacta
    19921 ttgcttatta gcgaaatatt ggcattttat tatgctaatt tttatataat ccgttgtatt
    19981 taattattta atatgctaaa ataaataggt atggtgaatg gttttatttt ctataatata
    20041 aaggtggggt tttatgtcaa ttaatgtaga ccttattaat ccttttgttc agggaacaca
    20101 agcgatttta agagatgtat gccaggaaac acccagactc ggaaaagttt atttaaagaa
    20161 gtcaccttat gccagtggtt ctattgtagt tgttatagga cttactggag atataaaagg
    20221 acaggttatt tttagtctgg acaagcaagc agctgtaacg atatcatcaa agatgatgat
    20281 gggattagaa gttgaagaat tagacgaaat ggcaaaaagt gcaatttcag aattaatgaa
    20341 tatgatttta ggtcatacgg caacgatatt ttataataaa ggaatttttg ttgatattac
    20401 accaccttct ttatttgttg gaaacgatat tcagatttct atttccaaga tacaaaccat
    20461 atgtattcct ttacatttag aagatggtgc tatgtttgaa attgatgttg ctattcagga
    20521 aaattttagt cagtgagatg gttgtaaatg aacattgtct tattagagcc agaaatacca
    20581 caaaacacag gtaatatcgc aagaacttgt gctgccattg gtgctgattt gcatttgatt
    20641 aaacctttag gtttttcaat agacgataaa catttgaaaa gagcaggatt agactattgg
    20701 catttattga atgtttatta ctatgaaaat tttgaggatt tcaagtctaa acaccccgat
    20761 ggcttgtatt ttatggctac aacaaaagct caaaaagtct atacggaagt aaaatataca
    20821 aaagattgtt ttattatttt tggtaaggaa acagcaggta ttcctgaaag tatattaatc
    20881 gattatccgg aaacctgcat caggattccc atgagagaag aagcccgttc attaaattta
    20941 tccaattcgg tagccattgt tgcttacgaa gcaatgaggc agttaaactt tgaaggttta
    21001 ttaaaagaag gagaattaag agattacaaa tggaacgaaa tccccggtaa ataaccgggg
    21061 atttttttat ggtaattagc ctctgaaagt gtttttcagt acacgggtta gtagtttaaa
    21121 catgtatagg ctattaactc gtgaattttt atattgaaac tttataaaat gttgaagatt
    21181 aaaaatttat taattagaat aaaattatat gaattcgtat aatttaataa taatttgatt
    21241 aaaatccctt gtgcttgtct taagccaatg gagcatctta tttatatctg acgggtatag
    21301 aaggagatta gttacaatta aaacagactt ctgttttaaa tgtcagatta taatttttta
    21361 ataaagcaga tgaaaggttg gtagcaaaaa aattgttttt accgggatag taatatagag
    21421 actgaaaaac gggaagcttg ggctgaggta tttagcgtta aaggagatcc caaattttgt
    21481 aagggttggg aagaacgaaa cagaagaaca ttaaatttat ttttatatgg aatttatacc
    21541 tttaaagaag aggagatttt aaacgaaaaa actaattaca gctcttttat tgatagaagt
    21601 tttatgtttt gttttttctg cttgtgggtc aaataaaagc agtgctcggg aagaaaaaca
    21661 acaagtgaca gaaaatacgc ataatgaaga tataatgtca agtgattttg catcaaatga
    21721 atcttttgat aagagtgatg ataataggag tacggaagta gaattaccag ctcaaaagca
    21781 atttgttgat agatcgtata aattatttta tgggaaatgg gaaataacag agattgttgg
    21841 agaacactca aggttcggtg catattagaa tgctgaagaa ttaataggaa cgacactata
    21901 ttttaattat gatcagatac taatagacaa caaagtggca gtatcaaatc ctaactatag
    21961 gattgcaatt gtaccttcta ataatagagc atacatacct tatctgcctt ctcataaaga
    22021 tttggggata gaaggtccat attttatata tgtttatgtt tctacaaaaa taacatcacc
    22081 aattataggg aatagaattt tttataaagg aggataatac tcttttcctt ttttataacg
    22141 atgcatttca taaagttgaa agaattgaac atattccaaa tgtagaagat aattatatta
    22201 aaattgtatc aaatggatat gagtaagaga ttttatgtca gtaagaaaga agttaaatat
    22261 aatttatcgg taagtgcaag tctgtcggta atgttaatag ggtgtcattt taatgtaaat
    22321 actgaagata atagtgatat gactaatagc atataagtaa aaaatgactt tgaaaataat
    22381 gatgtaaaaa atcaagaaat gatcattatt atctcggata gtaataatgg tatgccaaca
    22441 gtaccgaaga aggaaagaga aaaactactt tgtttattga ttgatttatg tagaaggcct
    22501 ttttattgat actgcggaag aggtatggga ttttttatat actcgttctg gtgccaattt
    22561 atatccacca agtaaagcca gtgaaaattt attaaggagt ttaagggatg acgcgagttt
    22621 gaaggtgtaa agccaaaggt tgtagacttc tatttaacga aagaggagga agactgtata
    22681 tttatgaata tgacgaactg aaccgccttc gttattctat agacccactt tttggggtag
    22741 aagaatacca gtacgacagt gaaggcaaca acacagccat tatagaccgc tggaacagca
    22801 aaacagagtt tgaatatgat gcgcttaatc gtttagtaag aaccactgat ccttatcaaa
    22861 acattactcg aaatttatat gctgatgact ttagccctaa actttcaact gttaatgcag
    22921 gtggaagaag tgtagaagtt tacagaacag caaccataga tgcccggggc tttacctgga
    22981 cttcctacta cgataaaaaa ggacaacttg tcatggagaa agatccagcg ggttatgaaa
    23041 agtgctatgg ttatgatgct gcaggcaata aaatctggga aacagataaa gaaaaacatg
    23101 taaaaacaaa ttacgaatat gatggttttt atcgtttgaa gagggtagac agtcctgaag
    23161 gcataagagt agaatatact tacaatgctt tagacagtat tttaacccaa accattggtg
    23221 ggagccaaac cactgtttac acttatgatg gtttaaaccg cttaacatct aaaagagatc
    23281 cttctggaag agtagaaagt tataacagct atgacaatgc gggcaatctg ttaagtagaa
    23341 ccgacagaaa tggaagaaca accagttatg aatacgacag tttttaccgc ttaaaatctg
    23401 aaactgtaga tgatgcttat catagccaga ggacctataa gtacgacaaa gtaggcaact
    23461 taatagaaga aaccagcagg tcaataagcc agtacagtaa agtaatgact tatgatgact
    23521 taaacaggct gttaacaaaa gaactgcctg gaggaaataa aatcgaatat acctatgaag
    23581 atgcagagca taaagaaatt atgacagacc ctgcaggcaa taagaccatt tactcttatg
    23641 ataaaatgaa ccgcttagaa attgttacca cggatgagag agatacggtc tatacctatg
    23701 atgctaacgg aaacaggaaa agtcttacgt atccttcagg aataacagct ttatatgaat
    23761 acgattcaag aaatttgctg gttagattga cgaattatac aaagatgcag gtaaagactg
    23821 cagaaagcat agaagacaaa ggagaatatg aaagtcttac agttactgca gcagtatatc
    23881 attatcctgg aaaaggaatt gaaaagatat acgaatacca atatgacggt aatggtatgc
    23941 aaacctgtaa gatagagcct aagggaaaga cagaatttat ctatgacgat gcaggaagga
    24001 taaaaacagt catagagcct gatggaaaaa aacgacttac acctatgaca gctttggaaa
    24061 cagaaagacc caaagaactg aaggagacgg gctgttagta gatatacaat atatctatga
    24121 caacagcaac cgtttggtta agactataga aatgaataat aatatggtta ttaataccga
    24181 atacggttat gataataatg gaaatcaaat atccgtcaaa gagtttagca gtagcggcaa
    24241 taaaacatca acttatgaat atgacggctt taaccagatg gtaaaagcca gaaccacaga
    24301 aggcaagaac ataagctgta actatgatgc cctgggctta agagtatcca agaccgcaga
    24361 tggaaaaacc acaaactatt actaccgcta tggcagcgtg atattggaaa aaaacaggtg
    24421 acaattctat aagaaacgta gtgggagtga cttaattgca agaggtggca aagaagatat
    24481 tctgtactac gtttataacg gtcatggaga tgtaatagaa ttgcagtcaa gcggcggagg
    24541 tatcgtaaat gaatatgact acgatatctt tggtaatcca accttagaaa acgaagctga
    24601 aggcaaagaa aacccctacc gttatgcagg ctattactgg gataaagaaa caggctacta
    24661 ttatttaaga acaagatact atgatccaga aatagcgaga tttataacag aagacagctt
    24721 tacaggatac tatgatgatc ctttgagcct gaatttgtat acttactgca tgaacagccc
    24781 gataaattac tgggatcctg atggacgtta ttatgtaaca gacccagtta caggagaaaa
    24841 aatacatgca tcggaatggg agagtagaag gaattttaga gctaaagtcc gagatagtca
    24901 aggaagagga gtttttgtaa gtactggggt cagtgcatat gattgggcac tccaaaacaa
    24961 agttaataag gggacgggta atggtggaac ttatacatat aaggataagg atggcaatac
    25021 agttacaaaa gaatggacaa taaccaatga cgaattttta gattctaatg ctttaactca
    25081 ggcggaaatc acagaaattt gtaggaaaaa gaatcctggc ttggttgaac gtggcttcga
    25141 taaggcaata tatgaatacg ctcagaagaa gggtataaac cccaaagtaa tattagcaac
    25201 acttgctcag gaacaaaaat ggtgtaaaaa tggaaggtat gataaagcat ttggggttgg
    25261 tccaggtgga aatccatcgg cttttgctga aaatgaaacg gggggtattg ctagttcagt
    25321 taatacattc ttgaatctat ttaatgaagg tttgaaaaaa cagaataatg gtggcttaaa
    25381 accaatgcat gtcaatcaag attataagca aaatggtgtt tggatggaaa cagatgctgc
    25441 tgctaggcaa gaatatggta tctcaggtgc tgaatggatg aatagaaatc caaagtatgc
    25501 tgagtactta aaaaatggaa ttgatgtaca gccagtaaat gcagcaatgt atgcaaaact
    25561 tagatataca ccttgggtag atttccccgt gcaaaactca catccattac aggaatggca
    25621 tgatttattt aggtcattct agtacgattg tatgaaatat ttgttgttat aaaaacacta
    25681 tgaaatagga gaataagatt atgaagagaa aaattaatat tctattgatt gtttttacaa
    25741 ttgctttggt cttttgtagc tgtagcataa aacagaattc atcggaagga agtcttaata
    25801 ataatgcgaa tagtattgaa tctcaaaaaa agacttcagg ttctttgcag gatttagatt
    25861 caaataaaac tgcgataaac ccagaggcat ctccgagtaa tcaaactata aagccaagca
    25921 ataatagtgt tccaggtcta aaggaagatg attttggggt tgtgtttagt aatgctttag
    25981 taaaggttac tgataaccct gataaaatga ttgaattatg gggcagaggc aaggtgaaaa
    26041 atgatgatga ggcatttatc ggaactgacc catctgcaaa gtataggagg tatgagtatt
    26101 actatattaa agagaattca ggcataaaag cgcatgttag aagaggagaa attgactaca
    26161 tatattttat ggatattact gattggtata ctaaccgggg tattaaaaaa ggagatagct
    26221 ataacaaaat ggttgaagct tatggaaagc ctactaaaga aagtattgaa gattctgaat
    26281 tagtttgcga atatcctcta aaaaatgtta gtttgatgtt caaatttgat aaagacaaaa
    26341 atatttccaa tattacgatt tggaactact taaattaata ttttgcaaat gactgtctgc
    26401 aataacagtc attccttacc cggtaaacca ataaaacagt agccgggttt ctttatgacc
    26461 attattataa atgtaacaca aaatgtaaca ctaatatatg acacataata atgactctga
    26521 tagaatatag ttatcataat aaaaggagga attatccatg gcagatactt tcacaatccg
    26581 catcagcaag gacacaccag cctttgagga gatggacaag gcagcagagc agcttggctt
    26641 aagccgtaac caacttatta tattatagag ggaattaaga tgatgtgttc atgggatgca
    26701 catttttaca aaaaaataaa gcagtacagc agcaccttca ggcttccagt tccgttggtc
    26761 atgcagaata tgctaataaa gcgccttgca gaggactacg caaaagagca gatttggggg
    26821 ttcacaggca aggtaatgcc ggaattcatg ttcaatgaac gtggacccat tacaggcggt
    26881 gagttgttca agacactttc tgaaatggca gtaaaggatg aaatcagaga aagggtcaga
    26941 ggcattgaag agcatattag gacaggcttt ccagtaccaa aggatgaaat ggatttttat
    27001 gaagcacaca agccaaatcc tgtaaaggtt gacccgcaag aggaagaacg taacagacag
    27061 ctttttgaaa agtatggtga gcctgtgtcc atagctttct gggcggatga aaccagtatg
    27121 gaagaagcac tggaaatgct tgagcaagag gaaaagaaaa aggggcgtgg caagaaatga
    27181 gcaagaaaca atatattgtg cgatgccccc attgcaatca cagagtattt gatgccgatt
    27241 atgcagatgt cgaaatcaaa tgcccggaat gcaggaaagt ctttgaagta aagtgaaaag
    27301 tgaatagatc tggcacagag ccacaaaggg agcgaagttg gactcaccta tagagcctgg
    27361 cagatagtct aaaaaactat ttgtcgggct ctatttattt tcccggcaag cgaagtgagg
    27421 tgagaaagga aatgaaccaa tatgaaaaca tcccaaagga actgaagcaa ttaccacagt
    27481 gggtatgcca tagaaataaa gtacccttta atcctgctac aggaaaagca gccaaggcag
    27541 gacagccaga tacgtgggca aggtttgagg atgctgtaaa agcttctggc tcttttggcg
    27601 gtatcggctt tgaattcaat aataacggta ttgtaggcat tgaccttgat aaggtaattg
    27661 ctgatgatgg aactgtttct gatgaagcag acgggattgt ggcaatgctg gacagttaca
    27721 cagagtattc tcccagtggc aaaggacttc atatattcgt caagggtgat atctctgtag
    27781 atggcaggaa gaaaggcttt attgaaatgc ataaagccaa aaggtatttt accatgacag
    27841 gtaatgtcta tggtaataaa aagcctataa atgagtacac agagcaagtc aaacagttat
    27901 ttggtaaata tttctccgat tctaaatcgg gaaaatctgt tggtacaaac aatccttgta
    27961 tcagttctgc aaaggactat ctttccatcg gacttgccaa ggatgctgtg ttcaaggctc
    28021 tttgggatgg tgaataccaa agtgagaagt gtaccagcga gagtgaagca gaccttgcac
    28081 tgatgggtaa gctgctctat tggtgctccg gcaatataga tgcagccatt gaagccttta
    28141 tcaaatcccc ctatgtagca ggaaaggatg acaagtacac aactaagctg gaacggtctg
    28201 actatctcca aagaaccgct gcaaaagcaa tgcaaggctt gacctccact gctgccgggg
    28261 atgatgagca gtattgcaaa cagcaggaat tcacccttga tgatatgggt aatgccagaa
    28321 ggcttagcag ttataagtac agaagttata tcagtcagaa atgttaagaa atttgtgtcc
//
LOCUS       DAOSUD010000021        27385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1156_length_27385_cov_27.945993, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000021 DAOSUD010000000
VERSION     DAOSUD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 27385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27385
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_27385_cov_27.945993"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..597
                     /locus_tag="PLG49_04360"
     CDS             145..597
                     /locus_tag="PLG49_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ16586.1"
                     /translation="MTYLKKVLNNLMVDIFNDILTIEETALKKGAFSDLSVKEMHTIE
                     AIGMYQPRTMTEVAQDLKITVGTLTTAINRLVKKGYVERNRIEDDRRIVQVQLTKKGK
                     LAYRIHEKFHTDMINAMIDGLKEEEEDILVKALSQLSKFLKDKYHLNQ"
     gene            610..1587
                     /locus_tag="PLG49_04365"
     CDS             610..1587
                     /locus_tag="PLG49_04365"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOQ16587.1"
                     /translation="MYEVRIIGTGSFLPQQIIKNDSLSDLVDTNDEWISTRTGIKERR
                     ISSGENTSDLAIEAAKEAIKDAGINAEEIDLIVLATSTPDFIMPSTACLVQKGIKAYN
                     ATCFDITAACTGFIYALNIGYQFIRTGQSKTALIIGADTNSKIIDWSDRNTCVLFGDG
                     AGAAILQRTDKEGIILVDTGSDGRGDELLFCPGVHLKNPFMEEQEEKKQVMHMNGKEV
                     FRFSTTVVPQTIEKLLQYSSYHIEDIKYFLLHQANARIMEAVAKKMNIDPEKFYKNIH
                     KYGNTSAATIPIALNEMDKAGLLEKDDLLVLVGFGGGLTWGSALVKWNK"
     gene            1608..1838
                     /gene="acpP"
                     /locus_tag="PLG49_04370"
     CDS             1608..1838
                     /gene="acpP"
                     /locus_tag="PLG49_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ16588.1"
                     /translation="MIFEKIQEIIADQLSIDKNEIKPESSFQEDLQADSLDLFQIVMA
                     LEEEFELEIPNEDIEGIKTVQDAVNYIESKQA"
     gene            1883..2815
                     /gene="fabK"
                     /locus_tag="PLG49_04375"
     CDS             1883..2815
                     /gene="fabK"
                     /locus_tag="PLG49_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOQ16589.1"
                     /translation="MYRFWKEIGIQYPIIQGGMAWVADHHLASAVSNAGGLGIIAAAN
                     APVSYVREEIRKVKELTDKPFGVNIMLLSDNASEVAQLVCEEGVKVVTTGAGDPGKYM
                     DMWKKSGIKVIPVVPSVALAKRMERYGADAVIAEGCEAGGHIGELTTMALIPQVVDAV
                     KIPVIAAGGIADGRGMAAAFMLGASGVQVGTRFLVAKECSVHPAYKERVLKAKDIDTV
                     VTGRPTGHPVRILRNRLSRELLKLEKEAAPLEQFEALGQGALARAVKEGDVEMGSVMA
                     GQIAGLINKEQTCSEIIEEMFNEFNALINRKIDI"
     gene            2844..3767
                     /gene="fabD"
                     /locus_tag="PLG49_04380"
     CDS             2844..3767
                     /gene="fabD"
                     /locus_tag="PLG49_04380"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOQ16590.1"
                     /translation="MKTAFIFAGQGSQYAGMGKELASHLKEAMDVFDQAQEVLDFDIK
                     ELCFEDKNARLNQTQYTQPAILTVSIAALRAIENQIQADLVAGLSLGEYSALVAGGAL
                     DFKDAVSLVRKRGQFMEEAVPQGTGGMRAVLGLSGEEVDAVIATIEDGIVEVANYNCP
                     GQIVIGGEIKALNSAEEKLKEAGAKRVIPLSVSGPFHTSILKGAAEKLRKELEKVTFT
                     EPTIPIISNVNADYADIKEIREILTKQVMSSVYWEQSIRKMLEDGVDTFVELGPGKVL
                     TGFIKKIDRNVTVCNVEDISSLEKTLELLGR"
     gene            3770..4519
                     /gene="fabG"
                     /locus_tag="PLG49_04385"
     CDS             3770..4519
                     /gene="fabG"
                     /locus_tag="PLG49_04385"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOQ16591.1"
                     /translation="MDIRKTALITGGSRGIGKAIAIRLAEKGNNIVFSYVSNEEAAQK
                     TLEEIKSKGVKVLAFKADVSSYDECQKLIDTTVKEMGSIDILVNNAGIIRDNLIMRMK
                     EEEFDEVIRVNLKGVFNCIKHVSKIMLKQKSGRIINISSIIGLIGNIGQINYAAAKAG
                     LHGITKSAAKELASRGITVNAIAPGFIESDMTNTLADKYKEAIIKAIPLGRIGKAKEV
                     ADLVCFLASEEASYITGQIICVDGGMAMGSI"
     gene            4534..5766
                     /gene="fabF"
                     /locus_tag="PLG49_04390"
     CDS             4534..5766
                     /gene="fabF"
                     /locus_tag="PLG49_04390"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOQ16592.1"
                     /translation="MRVVVTGMGCVTPLGNSIDSFWANIKEGRCGIDFISRFDTENFE
                     VKLAAEVKDFDPSLYMDKREAKRMDLYSQYAVAATKMAIDLSEIDLEKVCKERFGVIV
                     GSGIGGIGTIEKEHEKLLNKGPKRVSPLLIPMIIINMAAGNIAIQFGAKGICSTVVTA
                     CATGSHAIGEAYEMIKRGKADIIIAGGSEAGITPLSVAGFSSLQALTTTTDINRASIP
                     FDADRNGFVMAEGAGILILESLEHAQKRGARIYAEIVGYGATCDAYHITSPSIDGEGA
                     ARAMKEALEEAGISPDEVSYINAHGTSTPLNDKFETLAIKQVFGEKAKRIPVSSSKSM
                     TGHLLGAAGAVEAIVCIKALEEDFVPPTINYKNPDPECDLDYVPNIGRKQELNYALSN
                     SLGFGGHNASLLFKKYKN"
     gene            5778..6236
                     /gene="accB"
                     /locus_tag="PLG49_04395"
     CDS             5778..6236
                     /gene="accB"
                     /locus_tag="PLG49_04395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOQ16593.1"
                     /translation="MDYKEIEHLMKVMNESQLTELEIEKEGFVLKIKKEKEIVPIASR
                     IETPIIERPVEIQNQISTVKVEKEVSGEKEGIIVYSPIVGTFYSAPAPGAEPFVKVGD
                     KVKKGDVLCIIEAMKIMNEIEAESDGEIVEIFVKNEEMVEYNQPLFRIKA"
     gene            6249..6671
                     /gene="fabZ"
                     /locus_tag="PLG49_04400"
     CDS             6249..6671
                     /gene="fabZ"
                     /locus_tag="PLG49_04400"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOQ16594.1"
                     /translation="MLNIEQIKEIIPHRYPFLLIDKIEELEPGIRAVGYKNVTINEYF
                     FQGHFPKEAVMPGVLIIEALAQVGAVALLSLDSLKGKTAYFGGINKARFREKVLPGDC
                     LRLEVEIINRKGPIGIGAAKAFVGDKKVCEAELTFAVA"
     gene            6689..8041
                     /locus_tag="PLG49_04405"
     CDS             6689..8041
                     /locus_tag="PLG49_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOQ16595.1"
                     /translation="MFSKILIANRGEIAVRIIRACREMGIETVAIYSTMDKDALHVQL
                     ADEAVCVGPPKGKDSYLNMENIISATILKGAQAIHPGFGFLSENSRFAEMCQECNIAF
                     IGPDAQMIELMGNKSRARETMIKAGVPVVPGSQGAVEDLEEALGIAENIGYPVMIKAS
                     AGGGGKGMRLAFSKDEFVKAFTTAKQEAKAAFGNDSMYIEKYIENPRHIEFQILGDAY
                     GNIIHLGERDCSIQRRHQKVIEEAPSPVIHPELREKMGKAAVKAAATINYKNAGTVEF
                     ILDKQGNFYFIEMNTRIQVEHPVTEMITGIDIVKEQIKIAAGLPLEYKQEDIKINGHA
                     IECRINAENPQKGFMPSPGTVKGLYLPGGFGVRIENALYEGYKIPPIYDSMIAKLITH
                     GRDRKEAIAKMKSALGEFIIEGIETNIDFLFDILHTEDFEKGNVDTGFIEKKFSFDKA
                     "
     gene            8064..8924
                     /gene="accD"
                     /locus_tag="PLG49_04410"
     CDS             8064..8924
                     /gene="accD"
                     /locus_tag="PLG49_04410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOQ16596.1"
                     /translation="MIKGLFKKTKYVTTSVPSQEKAPTPNIPDGMWVKCNSCEQIIYK
                     KDIEENNRLCPNCGFHFRMSAKERIKLIVDEGTFIELDKDLTTLNPLDFPQYEEKIRQ
                     QEEKSGIKEAVITGTGRIYGEEAVIAVMDSFFLMGSMGSVVGEKITRAVEEATERRLP
                     LIIFCASGGARMQEGIFSLMQMAKTAGAIARHDEAGLLYISVLTDPTTGGVTASFAML
                     ADIIVAEPGALIGFAGSRVIEQTIKQKLPEGFQRAEFLLEHGFIDMIIRRQDLKKSLG
                     KLLCLHKEVK"
     gene            8924..9871
                     /locus_tag="PLG49_04415"
     CDS             8924..9871
                     /locus_tag="PLG49_04415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOQ16597.1"
                     /translation="MSEVNQNLEQLENKLQALKEFSASHGVDLNKEIKAIEEKIHELK
                     IEAYRNLSPWDKVNLARHSERPTSLDYIDEIFEDFIEFHGDRNFRDDPAIIGGLARIE
                     GKVVTVIGHQKGRNIKENIKRNFGMPHPEGYRKALRLMKQAEKFKRPVICFIDTPGAF
                     CGIGAEERGQGEAIAINLMEMSRLKTPVISIVIGEGGSGGALALGVADKIYMLENSIY
                     SILSPEGFAAILWKDGSRAKEAAQIMKITSEDLKNFEIIDDIIKEPLGGAHKDINEVS
                     KRIKEIIIKDLKELGNLELDQLLNRRYMKFRKMGKYQNN"
     gene            10088..13351
                     /locus_tag="PLG49_04420"
     CDS             10088..13351
                     /locus_tag="PLG49_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ16598.1"
                     /translation="MRVFRKKTKSTSLVTFLLALALVLTSFPAIPVQAATEDWSKQEQ
                     QGTYTSTESTGYGDAYIRIGDIDNFGNGWSGGFDPFSGQVGTYTKIDNSQSYNGTDKL
                     DSVNTVDFRYSLPNPYDVNYVISNARIEFYGTGFKAGNDYKVTMKFGDDEIRLTELEV
                     ILKFYDLKEGQAQFIPYRLPHRVLSYLESGAFSLTIKAFDSEGKPANFALDFVKLIIN
                     FQDLNNYAYIQGETYLAGTYNPNAPDGAQGTFVPWVTINAVGSNQVWYSEEDGHFNFK
                     VTPGKVVLIVSKFGYKTQVITLDLAPGQTIEYDIIMESGGGYADISIGAEDIDSLRNV
                     RSIPKYLVGDNLGQARVDKIFSSPIKLTKDPDGKIDPYVEFDIKGMQLTEKKYQFIKV
                     SNPSKVPPMPGPKDPNWKDLGEGTKTIIEPNTAEAYPDFMWAEKNHITVKHYEYNPKE
                     IFGKEIDYKEDTNPKVYVTGKKNVLVANTNASGYHDGDDDQPAFFRNDANAGGKAMKA
                     WGYLVLGPEVNKKQAIVLGIEANTGAYGYIEVGGKKIVFADGKNNGKNVISKEVSVEL
                     ERGKIYPIYMEWYAENGTAGTFVPVYKTNNSEWRQIPQQWFYASSNEGPGQKGQQFET
                     SALYKEVAFPKENGYYYVAVRANTAFAPEATHGLYGPFLIGDAEVEEEYTIELVPTQL
                     FLNVDQVGTVTAKITPEGYQGEIKWTITNNKDAKKDVIEFTGNSTDLVKYVKGLQPGE
                     ATIRATLDNNATASCQVIVLENVKGSISLPNYLYIKEGTTKDLAAIVNLGDNESVKWE
                     INGKVINNNKYNDEYIKVGSSKKNVLTIKGEKAAEDVYNIIARSADGKIESNVCKVYV
                     YSNLNQHSYFVGESISLEELFPGLPTDRPDEVEFKVALGNDKVKINGGNVDAVKEGTA
                     KVTATIKPSEDAENQEPEVIETNLRVFTVVFKNVTGQVFPNRATTTLVFEVTDGEAGY
                     VNVGRDLKVKVNLNDLKDIATAYAINNIQNNGQAQDIVIDYDTKTFTISVASEGNKEL
                     VIFMDLTPNPDMKVAEYNQLVKDDEKVPATISVTPVGGPTVTEVDEDLDIAYSTIIR"
     gene            13458..14156
                     /locus_tag="PLG49_04425"
     CDS             13458..14156
                     /locus_tag="PLG49_04425"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOQ16599.1"
                     /translation="MKKIGIIGAMEEEVIIIRDKMDNKEAYKIAGMDFYKGFLNNTEL
                     VIVQCGIGKVNAAVCTQALIDHFNVDYIINTGVAGGIYSQLEIGDIVISEDAVQHDFD
                     TRAFGYPLGVIPRMEESFFRADDHLVQLAHQSSKNLNLKANVYIGRIASGDQFISDVN
                     ENRRIWENFNAYCTEMEGAAIAQTCYLNKIPFVIIRAISDKADHSAEVNFQEFVNEAA
                     KNSSDLIIEILNNI"
     gene            14321..14989
                     /locus_tag="PLG49_04430"
     CDS             14321..14989
                     /locus_tag="PLG49_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16600.1"
                     /translation="MNFAFLVELIPIFMYLTAISFGLGMLSLMFLGIRIRYLKKALKK
                     WTKENKRISNDPTLDKIVKIYRMYNEQGCEKINTSAIIHGNYYKQKILFIPIYYWEQF
                     ISKSEILCLFFGLITTFILVSCQKDNPFDPLIFGTGTFIILVILEMFLSLDAKKKYLF
                     AQIEEYLDNSYRYSLQRTMVTAEYFDKEKIELFDMKEGKEEEIKDEEIEWVIKQFYDV
                     ENEV"
     gene            15063..16145
                     /locus_tag="PLG49_04435"
     CDS             15063..16145
                     /locus_tag="PLG49_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ16601.1"
                     /translation="MEDPKVKLPITNEYGFYEIRLESIGGLGANLSGKILGELGAIYL
                     GLNSSSFSSYGSEKRGTPVKAYIRWADPSREININSPVERPHLLGLFHERLAGKIPVM
                     AGVGPETIVVVNTDASPDQMRDRLKMHAGTLICVDALKIAMEAKTRVNMVMLGAMAKA
                     SGFIPLEALKQIVEDTLGKKYPAALKGNLEGIEKGYENIQMKKFEDDGKYPYQEYKEV
                     KREWGYDNAPIGGINPHFGSTISNDLSGSREGYIPVFKKEACINCGMCDSTCPDMVYQ
                     FVLGEYKGKTSMVNLGPDYHHCKGCLRCVEVCPTDALTAGIEREVDIWKIHVRNQELI
                     VDKLEFEDVGPNSWMRSESIVTNKLH"
     gene            16158..18404
                     /locus_tag="PLG49_04440"
     CDS             16158..18404
                     /locus_tag="PLG49_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ16602.1"
                     /translation="MVEQKVVFESGNELASYAAKQINYHIMGYYPITPSTEIAEYLDL
                     MKSEGEHDIVMIPADGEHGAAGICYGASTGGGRVFNATSANGLMYALEQLPVQSGTRF
                     PMVLNVACRTISGPLSIKGDHSDIMYALNTGWIILFAKDPQRVYDFNLCGLKIAEKVK
                     LPIIIAFDGFFTSHQKRRAQIIAKDEDVQNFIGKPNPEYHTLDKENPIIVGSYMNEPD
                     LLNNKYQLHLAMEEARKVIPEIFAEYQELTGRAYQMAEGYRMEDAEAALFILGSSYDT
                     ARMAVDKLRKEGKKVGVFTSNVLRPFPKKELYEICKNVKSIIVADRQDSYGAEGGNMT
                     LELKAALQDYKANIEVASLVYGLSGRDFYIEEGIEMLNRAYETAQKGKVEKRFDYFGH
                     YKGKQDYKIERYFEPITGEKARPGITSVEFDEATGRFKVKGGQVKDSTRMPKRWTAGH
                     GACPGCGIPVNVNMLLRGIEGEVVLLFQTGCGYVVSSPYPKSAFRVTFIHNLFQNGAA
                     TLSGLVEMFHEKKRRGELPQGDNFTFIMVTGDGGLDIGMGAAIGAALRNHKMIIMEYD
                     NGGYMNTGYQLSYTTPMGAKSSTSHVGPAQKGKLTFHKDTPQIFAATNIPYVATVSES
                     QPTDFIKKAAKAQKYANEYGLAFIKALSACPLNWNDNPRYERSVIDAAVNCCYHPLYE
                     IEEGITTITYNPEERNKKIPVIDWLKMMGRTKHLVKPEYADIVESIQKEVDRRWERLK
                     ARHANPLL"
     gene            18498..19508
                     /gene="pdaA"
                     /locus_tag="PLG49_04445"
     CDS             18498..19508
                     /gene="pdaA"
                     /locus_tag="PLG49_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOQ16603.1"
                     /translation="MKKLFMMFFIFMVITVFLTACDSKDIAMEDEDKQVIENSQEEIQ
                     NEDTEMEEANEEELEETIEEKQEEDAEPKNEDIENPVNKDDKGNTGNQDKKEEVSKNE
                     PGTTTKLSNDLKSWWFKRNNEHLPPGAQSEIDLSKYDAYYLGNISEKKIYLTFDEGYE
                     NGYTSKILDILKENNVKAAFFVTKSYIKSQPELIKRMVDEGHVVGNHSVTHPSLPSKN
                     DEEVKYEITETARYFEEITGKDMDPFFRPPAGEYSERTLQITKDLGYKTIFWSMAYKD
                     WDVNDQPGKEAALKHVVDNHHPGAIILLHAVSSSNTEALNDMIQYLKAQGYDFASLYE
                     IN"
     gene            19597..20940
                     /locus_tag="PLG49_04450"
     CDS             19597..20940
                     /locus_tag="PLG49_04450"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOQ16604.1"
                     /translation="MGKILFDYSNAGIKDEEIKNALWQLKDAHNMLHNKTGAGNDFLG
                     WIDLPVNYDKEEFNRVKAAAKRIRENSDVFIVIGIGGSYLGARAVIEALGHSFYNMLP
                     KEKRQGPEIYFVGNNISGTYVQHLLDLIEGKDISVNVISKSGTTTEPGIAFRIFKKYV
                     EEKYGKEEAKKRIFATTDKEKGALRTLANEEGYETFIIPDDVGGRFSVLTPVGLLPIA
                     VAGIDIDKLMEGAKDGREEYQNEDFESNIAYQYAIVRNILYRKGKQIEILANYEPSLH
                     FISEWWKQLYGESEGKDQKGIFPASVDFSTDLHSLGQYVQDGSRILFETVLNVEKPKL
                     DITLEAEETDLDGLNYLAGKTMSFVNNNAFKGTLLAHVDGGVPNLVITIPEINEYYIG
                     KLLYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEELRNELQKRIK"
     gene            20972..21490
                     /locus_tag="PLG49_04455"
     CDS             20972..21490
                     /locus_tag="PLG49_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOQ16605.1"
                     /translation="MDKEQRKNIILAMLKESNKPLAGKYLAEKLGVTRQIIVQDIALL
                     RAENHNIMSTARGYVFYDRKQYANKRVITVSHKKEAIEDELITIIDLGGTVLDVIVEH
                     PIYGEITGSLMIESRRDIKEFMEIMKKNETVPLLTLTSNIHMHTIEAKNEEILDEIEY
                     ELKRKGYLLIDH"
     gene            21610..22833
                     /gene="eam"
                     /locus_tag="PLG49_04460"
     CDS             21610..22833
                     /gene="eam"
                     /locus_tag="PLG49_04460"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular transferase
                     activity, transferring amino groups [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HOQ16606.1"
                     /translation="MTENEKRIISLKRAEELKSRIKDYTMKKNSIKTGFTHSEMIKEN
                     KRRIMKVLNASEKEWDDWKWQLSNRITDVHTLAKILNLSDEEIHQIDMIGKKFRWAIS
                     PYYASLMDPDDPFCPVRRQAVPSIEEMVEAGEEDPMAEEFTSPVEGITRRYPDRLIVK
                     VTNQCAMYCRHCQRRRCIGQKDLALPMAALRKCVEYIRNEEEIRDVLITGGDALLISQ
                     KNLEWLLSELHEIPHVEIIRIGTRTPVTMPQRITKELCDVLSKYPPIYLNTHFNHPME
                     ITEDAKKATDMLCSAGVVLGNQAVLLKGINDDVHIMKKLNQELLKIRIRPYYIFHPKE
                     VVGTAHFKVKVQKGMEIMEKLRGYTSGLAIPTYIINAPNGKGKTPILPNYILSWEENK
                     IKLRNWEGEVFYYPN"
     gene            complement(22826..23410)
                     /gene="yyaC"
                     /locus_tag="PLG49_04465"
     CDS             complement(22826..23410)
                     /gene="yyaC"
                     /locus_tag="PLG49_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOQ16607.1"
                     /translation="MFAHKKIESPTYIDTKSNLCLKEFSNCFLEYFSKKIPYRKETII
                     LCIGTDRATGDSLGPLIGYKLKNISYPNVFVYGSLDEPVHAKNLQETLDMISKNHLDP
                     LIIAIDACLGKMEHVGYITIGEGALKPGAGVRKDLPPVGDLFITGIVNFSGFMDMLVL
                     QNTRLSLVMKMADIISAGINYSFMKYFNSNKLIN"
     gene            23609..24829
                     /locus_tag="PLG49_04470"
     CDS             23609..24829
                     /locus_tag="PLG49_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ16608.1"
                     /translation="MSEFLNDSFDSGDELKEDITDTSPFLENAKLIGESPTGLKIYLE
                     DYVYTYLYKYAKFYRGEERAAALIGTFMEDSGQMCAVISGAVQIPPETKGETGELFLE
                     THLEEIQKQMEKYFPDKELMGWMHTQPGYGIFLTTQDLLIQKKYFKEAFQTLMIIDPI
                     EDMDAFFAWEGEELRSVKGYYLYYHKNEQMQNYMIANKIGLSSEELNDREDVVMHFRK
                     KDRMRRQEIQNKKIMRRVTTLSCVLFLMCATMAVNLIQYKERLARLETELDHIGSNYS
                     DILKRLDKENLQVVFAEENIEDIITETEEYAVISAEATEIDKGNEDNNKNEENTANAD
                     HENREANNETKEETAEPEAVDYYTVQKGDNLIKICYQHYKTTNMLGKIVEMNNLESPN
                     KIYEGQVLKLPRLQ"
     gene            complement(24854..26662)
                     /gene="pepF"
                     /locus_tag="PLG49_04475"
     CDS             complement(24854..26662)
                     /gene="pepF"
                     /locus_tag="PLG49_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOQ16609.1"
                     /translation="MGKNRRNALPKRSEINPQYTWKLEDLYPDDEACKKDMEKLTASM
                     SQLSEFQDQLTLSSENLYACLSLYDQLSQMVEKIYVYTHMKLHEDTSNNTYQALADKA
                     KALSVNFSSVSAFIVPEILASPEESLKNYLKENEDLKLYTHFLNDLLRQKEHTLSKKE
                     ELLLAKASEIGNIPQSVFTMLNDADIRFPSIKGESNEEIEVTKGRYIQLLENPNREIR
                     KAAFKSLYSSYIKQKNTLAAIYNGSVKKDVFFASARKFSSSLESALFTDNIPVEVYDN
                     LIQTVHEYLPCMHQYVALRKRMLGLDQLHMYDLYVPIIKDMDIKVSYEEAKDIVKEGL
                     KVLGKEYISIVDKAFNERWIDVYENEGKRSGVYSWGAYGTHPYILLNYQDNINSVFTL
                     AHEMGHAIHSYYSDNTQAYVYAQYPIFLAEVASTVNESLLMQHLLNTTNDQKKRAYLI
                     NHFMEQFRGTLFRQTMFAEFEKITHEMVEKGEALTSDSLCFIYRDLNIKYYGPDIVVD
                     PEINYEWARIPHFYNAFYVYKYATGYSAAIALSNKILNNEENALENYITFLKSGSSDY
                     PINILKKAGVDMTSPEPVKKALDLFKNLLMEMEKMQ"
     gene            27092..>27385
                     /locus_tag="PLG49_04480"
     CDS             27092..>27385
                     /locus_tag="PLG49_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16610.1"
                     /translation="MTVLETIEKYISDFSSLENAEERYCISLLYDYCKYKEPQLEIEE
                     LDEQFFDKFFLYWLPGRNKMLTGDRLYYLFPTIKKYYKYLRKNYKIKELNLIKS"
BASE COUNT         9759 a         4100 c         5745 g         7781 t
ORIGIN      
        1 ataccatgaa agtctggaat atatttttaa aaagaagaat acaaagtctt agggcataat
       61 tttttattga caaaaataaa tttattatat atactttgat tatcaaaata tttgagtatc
      121 aaagatactt gaggtgaaat taaaatgaca tatttaaaaa aagtattaaa caatctgatg
      181 gtagacatat ttaatgatat cctgaccatt gaagagacgg ctttaaaaaa aggtgcattt
      241 agtgatttat ccgttaaaga aatgcatacc atagaagcta taggtatgta tcaacccagg
      301 accatgacag aagtggctca ggatttaaaa attacagtag gaactttaac gaccgctatc
      361 aaccgtctgg ttaagaaagg atatgttgaa agaaacagga ttgaagacga taggagaatt
      421 gttcaagtcc agcttaccaa aaaaggaaaa ctggcttaca ggatacatga aaaattccac
      481 acagatatga ttaatgccat gattgatggt ttgaaagaag aagaagaaga tatactggta
      541 aaagccctta gtcaattaag taaattttta aaagacaaat atcatcttaa tcaataaagg
      601 agtttaaaaa tgtatgaggt tcgcattatc ggtacaggga gttttcttcc acagcaaatt
      661 attaaaaatg attctttatc tgatttagta gacaccaatg atgaatggat ttctacaagg
      721 acagggatta aagaaagaag aatatcatct ggtgaaaata catccgattt agctatagaa
      781 gctgctaaag aagccataaa agatgcaggc attaatgcag aagaaatcga tttaattgtt
      841 cttgctactt ctacaccaga ctttattatg ccttctacag catgtttagt acaaaaagga
      901 ataaaagctt ataatgccac ttgttttgat attacagcag catgtacagg cttcatatat
      961 gccctgaata ttggttatca atttatccgg acagggcaaa gtaaaacagc cttaattatt
     1021 ggtgctgata ccaattctaa aatcattgac tggtctgaca gaaatacttg tgtgcttttt
     1081 ggtgatgggg caggagcagc tattttacaa aggacagaca aagaaggaat aattcttgtg
     1141 gataccggtt ctgatggcag aggagatgaa ttgttatttt gccctggtgt ccatttgaag
     1201 aatcccttta tggaagagca agaggaaaaa aaacaggtta tgcatatgaa cggaaaagaa
     1261 gtattccgtt tttctacgac tgtagtgccc cagaccattg aaaaattatt gcaatacagc
     1321 tcttatcata ttgaggacat taaatatttt ttactccatc aggccaatgc ccgcattatg
     1381 gaggcagtgg ctaaaaaaat gaatatcgat ccggaaaaat tttataaaaa tattcataaa
     1441 tacggtaata ccagtgctgc tacaattccc attgcattaa atgaaatgga taaggcggga
     1501 ttattggaga aagatgattt attagtttta gtgggatttg gaggaggctt aacctggggt
     1561 tcagccctgg ttaaatggaa taaataaaaa attaggagga gtataaaatg atatttgaaa
     1621 aaatccagga aattatcgca gatcaattaa gtattgataa aaatgaaatt aaacctgaat
     1681 ccagtttcca ggaagattta caagcggatt ctttagactt gtttcaaatc gttatggctt
     1741 tagaagagga atttgaactg gagataccca atgaagatat tgagggtatc aaaactgtac
     1801 aagatgccgt aaattatatt gaatccaaac aagcataaag gtcagatgaa tccatctgac
     1861 ttttcttcta ggaggtaata aaatgtatcg attttggaag gaaataggca ttcagtatcc
     1921 cattattcag ggagggatgg cctgggttgc tgaccatcat ttagcatctg ctgtttccaa
     1981 tgcagggggg cttggaatta ttgctgcagc taatgcaccg gtttcatatg tcagggaaga
     2041 aatcagaaaa gtaaaagaat tgacggataa accttttggt gtcaatatca tgctgcttag
     2101 tgataatgcc agtgaagttg cccaattagt ttgtgaagaa ggcgtaaaag ttgttacaac
     2161 aggagcaggg gaccccggaa agtatatgga tatgtggaaa aaaagcggta taaaagttat
     2221 tcccgttgtt ccttctgtgg cgctggcaaa aagaatggaa cgctatggag ctgatgcggt
     2281 cattgctgaa ggctgtgaag caggagggca tattggggaa ttgacaacga tggctttaat
     2341 tccccaggtt gtagatgcgg ttaagattcc tgttattgca gcaggaggta tagctgatgg
     2401 cagggggatg gcagctgctt tcatgctggg cgcttcaggt gtacaagtag gaacaagatt
     2461 tttggtagcc aaggaatgca gcgtacatcc tgcctataaa gaaagggttt taaaagctaa
     2521 agacattgat acagtggtaa ccggaagacc tacgggtcat cccgttagaa ttcttagaaa
     2581 ccgtctgtcg agggaacttt taaagttaga aaaagaagcg gctcccttag agcagtttga
     2641 agccttggga caaggagccc tggcccgggc agttaaagaa ggggatgtgg aaatgggatc
     2701 cgttatggca ggacaaattg cgggcctgat aaataaagag caaacttgtt ctgaaattat
     2761 agaagagatg ttcaatgaat ttaatgcatt aatcaataga aagattgata tttaacggaa
     2821 atatagagtt atggagggag acaatgaaaa cggcctttat ttttgctggt cagggttctc
     2881 aatatgcagg catgggaaag gaactggcca gtcatcttaa agaagcaatg gacgtctttg
     2941 atcaagccca ggaagtttta gattttgata ttaaagaatt gtgttttgag gataaaaatg
     3001 caagattaaa tcaaactcaa tatacgcagc cagcaatttt aactgtcagt attgctgcat
     3061 taagagccat agaaaatcaa attcaggcag atttggtggc agggttaagt ttaggagaat
     3121 attctgccct ggttgctggc ggagcacttg attttaaaga tgcagtaagt ttagtaagaa
     3181 aaagaggaca gtttatggaa gaggctgttc cccaagggac agggggcatg cgggcagtcc
     3241 ttggactatc cggggaagag gttgatgctg taattgctac aattgaggat gggattgtgg
     3301 aagtagccaa ttataattgt ccgggacaga ttgtcattgg gggagaaata aaagcattaa
     3361 attcagcaga agaaaaactt aaagaagcag gtgccaaaag agtgattcca ctatccgtca
     3421 gcggaccttt tcatacttcc atacttaaag gggctgcaga aaaattaaga aaagagttgg
     3481 aaaaagtaac ctttacagaa cccacaatac ccattatcag taatgttaat gccgattatg
     3541 cagatataaa ggaaataaga gaaattctta caaagcaagt catgagttct gtctattggg
     3601 agcaaagtat cagaaaaatg ttagaagatg gtgtagatac ctttgtagaa ttaggaccag
     3661 gaaaggtttt aacaggcttt attaagaaaa tagaccgtaa tgtgaccgtg tgtaatgtag
     3721 aagatatatc atctttagaa aaaacattag aattactggg gaggtagtta tggatatcag
     3781 aaaaaccgct cttataacag gaggcagcag aggaatagga aaagcaattg ccataaggct
     3841 ggccgagaaa ggaaacaata tcgtctttag ttatgtaagt aatgaagaag cagcccaaaa
     3901 gaccctggaa gaaattaaat caaaaggcgt aaaagttctg gcctttaaag cagatgtcag
     3961 ttcctatgat gagtgtcaga aattaatcga tacaacggtt aaagaaatgg gttctattga
     4021 tattttggtt aataatgcag gtattattcg ggataattta attatgcgta tgaaagaaga
     4081 agaatttgat gaggtcatcc gggtaaatct taaaggtgtc tttaattgta tcaaacatgt
     4141 gagcaaaatc atgttaaagc aaaaaagcgg aagaattatc aatatttctt ctatcatagg
     4201 ccttatcgga aatattggtc aaatcaatta tgctgcagcc aaagcaggtc ttcatggaat
     4261 aacgaagtct gcagccaaag aactggcatc aaggggcatc accgttaatg ctattgcacc
     4321 aggttttatt gaaagtgata tgaccaatac tttagcagat aaatataaag aagctatcat
     4381 taaagctatt cccttgggca gaatcggtaa ggcaaaagaa gtagctgacc tggtatgttt
     4441 tttagcctca gaagaagcca gctatattac aggacaaatt atttgcgttg atggcggcat
     4501 ggctatgggc agcatttaga atggagggaa cttatgagag ttgttgttac aggaatgggt
     4561 tgtgttaccc ctttaggaaa ctctattgat agtttttggg caaatataaa agaaggcaga
     4621 tgcggtattg attttatcag ccgttttgat acagaaaatt ttgaagtaaa actggcagca
     4681 gaggtcaagg attttgatcc gtccttatat atggataaaa gagaagcaaa aagaatggac
     4741 ttatacagtc aatacgccgt tgcagcaaca aaaatggcca tcgacttgtc agaaattgat
     4801 ttagagaagg tttgcaaaga aagatttggt gttattgtag gctcagggat tggtggaatc
     4861 gggactattg aaaaagagca tgaaaaatta ctaaacaagg gaccaaaaag agtttctcct
     4921 cttcttatac cgatgattat tattaatatg gctgcaggca atatagcgat tcagtttgga
     4981 gcaaagggta tttgctcaac ggttgtaaca gcctgtgcta caggaagcca tgccataggg
     5041 gaagcttatg aaatgataaa aaggggaaaa gcagatatta tcatcgcagg aggttcagaa
     5101 gcagggatta cccctttgtc ggttgcaggt ttttcttccc ttcaggcttt gacgacaacg
     5161 acagatatta accgggcttc cattcctttt gatgcggaca gaaatggatt tgttatggca
     5221 gaaggagcag ggatattaat tttagaatct ctggagcatg cccaaaaacg gggcgctaga
     5281 atttatgcag aaattgttgg ctatggtgca acctgtgacg cttatcatat cacatcccct
     5341 agtattgatg gggaaggagc agcaagggct atgaaagaag ccctggaaga agcaggtatc
     5401 agcccggacg aggtatccta tattaatgct catggaacca gtacacctct taatgataaa
     5461 tttgagactt tagctataaa acaggttttt ggtgaaaagg ccaaaaggat ccctgtaagt
     5521 tcttcaaaat ctatgactgg acatttactt ggcgctgcag gtgcggtgga agccattgta
     5581 tgcattaaag ctttagagga agactttgtt ccaccaacga taaattataa gaatcctgat
     5641 ccggagtgtg atctggatta tgtgccaaac attggaagaa aacaggaatt aaactatgca
     5701 ctgtcaaatt cccttggttt tggtggacat aatgcttccc tgttatttaa gaagtataaa
     5761 aattaggagg gtgaagcatg gattataaag aaatagagca tttaatgaaa gtcatgaatg
     5821 aaagtcagct gaccgagctt gaaatagaaa aagaaggatt tgtattaaaa ataaaaaaag
     5881 agaaagaaat tgttcctatt gccagcagaa ttgaaacgcc aattatcgaa agacctgtag
     5941 aaattcaaaa tcaaatctca acagtaaagg ttgaaaaaga agtatccggg gaaaaagaag
     6001 gtattattgt atattctcca attgtaggaa ccttttatag cgctccagct ccgggagcag
     6061 agccttttgt caaagtaggt gataaagtaa aaaaaggaga tgtgctttgt attattgaag
     6121 cgatgaaaat aatgaatgaa attgaagcgg aaagtgatgg agaaatcgtt gaaatctttg
     6181 ttaaaaatga agaaatggtc gagtacaatc aacccctgtt cagaataaaa gcctaggtgg
     6241 tgatacacat gttaaatata gaacaaataa aagagattat tcctcacaga tatcctttct
     6301 tattaattga taaaatagaa gaattagaac cagggataag ggcagtaggt tataagaatg
     6361 taaccattaa tgaatatttt tttcaaggtc atttcccaaa agaagccgtt atgccaggcg
     6421 tattaattat agaagctttg gcccaggtgg gtgctgtagc tcttctttcc ctggatagtt
     6481 taaaagggaa aactgcctat tttggaggca tcaacaaagc acgtttcagg gaaaaagttc
     6541 ttcctggtga ctgcttaaga ttggaagtag aaattattaa ccgcaaggga cctataggga
     6601 ttggagcagc aaaagcgttt gtaggtgaca aaaaggtctg tgaagcggaa cttacctttg
     6661 ctgtagctta aacccaaagg gggatagtat gtttagtaaa attttaattg ccaatagagg
     6721 agaaattgcc gttagaatta tcagggcctg cagagagatg ggcattgaga ccgtagccat
     6781 ttattcaacc atggataaag atgccctgca tgtccaactg gcagatgaag ccgtgtgtgt
     6841 aggcccgcct aaaggaaaag acagctatct taatatggaa aatatcatta gtgcaaccat
     6901 cttaaaggga gcacaggcca ttcatccagg ttttggcttt ctttcagaaa atagtcgttt
     6961 tgctgaaatg tgccaggagt gcaatattgc ctttatagga ccggatgcac aaatgattga
     7021 attaatggga aataaatcga gagcaagaga aacgatgata aaggcaggcg ttcctgtcgt
     7081 gcctggctct cagggggcag tagaagactt ggaagaagcc ctgggaattg cagaaaatat
     7141 aggttatcca gtgatgataa aggcttctgc cggtggcggt ggtaaaggaa tgcggttagc
     7201 cttctcaaaa gatgagtttg taaaagcctt tacaactgca aaacaagagg ccaaggcagc
     7261 ctttggcaat gacagcatgt atattgaaaa gtatattgaa aatcccagac atatagaatt
     7321 tcaaatttta ggagatgctt atggcaatat tattcattta ggggaaagag attgttccat
     7381 acaaagaaga catcaaaaag ttattgaaga agcaccttca cccgttattc atccagaact
     7441 cagggaaaag atgggtaaag cagctgttaa ggcagcagct acgattaatt ataaaaatgc
     7501 cgggactgta gaatttattt tagataagca aggaaatttc tatttcatcg aaatgaatac
     7561 aagaattcag gtggaacacc cggtaacaga aatgataact ggcatagata ttgtaaaaga
     7621 acaaataaaa attgcagccg gtcttccctt agaatataaa caagaagata ttaagataaa
     7681 cggtcatgcc attgagtgca ggattaatgc agaaaatccc caaaaaggct ttatgccttc
     7741 tccagggact gtaaaaggtc tttatcttcc cggaggtttt ggggttagaa tagaaaatgc
     7801 cctttatgaa ggatacaaaa ttcctcctat ttatgattca atgattgcaa aacttatcac
     7861 tcacggcagg gatagaaaag aagcgattgc taaaatgaaa tctgccttag gggaatttat
     7921 tatagaagga attgagacta atattgattt cttatttgac attcttcata cagaggattt
     7981 tgaaaagggt aatgtggata ctggttttat agaaaaaaag tttagttttg ataaagccta
     8041 atcattagga ggtgatatat caagtgatca aggggctgtt taaaaaaacg aaatacgtta
     8101 caacttcggt tccttctcag gaaaaagctc caacccctaa catcccagat ggaatgtggg
     8161 taaaatgcaa ttcttgtgaa caaattattt ataaaaaaga tatagaagaa aataacaggc
     8221 tttgtccaaa ttgtggtttt cattttagga tgagtgccaa agaacgaata aaattaattg
     8281 ttgatgaagg aacttttata gaattagaca aggacttaac gacattaaat cctttagact
     8341 ttccgcaata tgaagaaaaa ataagacagc aggaagaaaa aagcggcatt aaggaagcgg
     8401 ttatcacagg aactggcaga atctacggag aagaggccgt tattgctgtt atggatagtt
     8461 tctttttaat gggaagtatg ggctctgtgg ttggagaaaa aattacaagg gcggttgaag
     8521 aagcaaccga aagaagactg ccacttatta ttttttgcgc atctggagga gccaggatgc
     8581 aagaagggat tttttcttta atgcagatgg caaagacggc tggagctatt gcaaggcacg
     8641 atgaagcagg attattatat atttctgttt taacagatcc tacaacagga ggggttacag
     8701 ccagttttgc catgttggca gatattatcg tagcagaacc aggggctctt atcggttttg
     8761 caggttccag agtgattgaa caaaccataa aacaaaaact ccctgaaggt tttcagagag
     8821 cagaattttt attagaacat ggatttatag atatgattat aagaagacaa gatttaaaaa
     8881 aatcccttgg gaagctttta tgccttcata aggaggtcaa ataatgtctg aagtcaatca
     8941 aaatcttgaa caattagaaa ataaattgca agcgcttaaa gaattttcag ccagtcatgg
     9001 ggttgattta aataaggaaa tcaaagccat cgaagaaaaa atccatgaat taaaaattga
     9061 ggcatacaga aatctttccc catgggataa agtcaatctg gcaagacaca gtgaaagacc
     9121 cacaagtctt gattatatcg atgaaatatt tgaggatttt atagaatttc acggggacag
     9181 gaatttcagg gatgatccgg ctataatagg cggtcttgcc cgtatagaag ggaaagtagt
     9241 aaccgttata ggtcatcaaa aaggcagaaa cataaaagaa aatattaaaa gaaactttgg
     9301 catgccgcat ccggaaggct acaggaaggc tttaagactg atgaaacaag ctgaaaagtt
     9361 taaacgtcct gtaatatgtt tcatcgatac tcctggggct ttttgcggta ttggagcaga
     9421 agaaagagga cagggagaag ccattgcgat taatttgatg gaaatgtcac gtttaaaaac
     9481 gcccgtcatt tctatagtca taggagaagg aggaagtggt ggtgcactgg ctttaggcgt
     9541 ggcagataaa atttacatgc ttgaaaattc tatttactcc atactatcgc cagaaggttt
     9601 tgccgctatc ttatggaagg atggaagccg ggctaaagag gcagctcaga ttatgaaaat
     9661 aacgtcagaa gatctaaaga attttgaaat aatagatgat ataataaaag agcctttggg
     9721 aggcgctcac aaagatataa atgaagtatc aaaaaggatt aaagaaatta taattaagga
     9781 tttgaaagaa ctgggaaatc tggaattaga tcagctatta aataggcgtt atatgaaatt
     9841 tagaaaaatg ggaaagtacc aaaataacta aaatggctaa aagatgcttt tagccattat
     9901 tgctttttga atataaattg cattattcct attctaaaag tttattaaat taaatttata
     9961 ctcatgacta aaaatttttt taaatctcta aaaatcatat tgaaatattc taaaaaatgt
    10021 attaaactat aatcaggtat acgtatctaa ttgttacaaa aaatgtttaa tttgtaggag
    10081 ggataatgtg agggtatttc gaaagaaaac taaatcgaca tccttagtta cttttttatt
    10141 ggcactcgca ttagtgttga ctagttttcc agcaatacca gttcaggcag caacggaaga
    10201 ctggagtaaa caagaacaac aaggaactta tacctcaaca gaatcaacag ggtatggcga
    10261 tgcatatatt cgtatagggg acattgacaa ctttggtaat ggatggtctg gcggttttga
    10321 tccatttagt ggtcaagtag gcacttatac taagattgac aatagtcaaa gttataatgg
    10381 aactgacaaa cttgattccg taaatactgt tgactttaga tattctcttc ctaatcctta
    10441 tgatgttaac tacgttattt ctaacgcaag aattgaattt tacggtacag gattcaaggc
    10501 aggcaatgat tacaaagtaa caatgaagtt tggagatgac gaaataaggc ttacagaatt
    10561 agaagtaatt ttaaaatttt atgatcttaa agaagggcaa gcacaattta ttccttacag
    10621 actcccacac agagtacttt catatttaga atccggtgct ttttctttaa ccatcaaagc
    10681 attcgattca gaaggtaaac cggctaattt tgctttagac tttgtaaagc ttattattaa
    10741 cttccaggat ttaaataatt atgcatatat acaaggtgaa acctatttgg caggcacata
    10801 caatcctaat gctccagatg gagcccaggg aacctttgtg ccatgggtaa ccattaatgc
    10861 tgtaggaagt aaccaggtat ggtattcaga agaagacggt cactttaact ttaaagttac
    10921 tcctggaaaa gttgttctta ttgtttcaaa atttggttat aaaactcaag tgattacatt
    10981 agatttagca ccaggtcaaa caattgaata cgatattata atggaatctg gcggaggcta
    11041 tgcagatatt tcaattggtg cagaggatat tgattcatta agaaatgttc gttcaattcc
    11101 taaatatctg gtaggagata atttaggtca agcaagagtt gataaaatat ttagcagtcc
    11161 aattaaatta acaaaagatc ctgacggaaa aattgatcca tatgtagaat ttgatattaa
    11221 gggaatgcaa cttacagaaa agaaatatca atttattaaa gtaagtaatc cttctaaagt
    11281 cccaccaatg ccaggaccaa aagatcctaa ctggaaagac ttaggagaag gaacaaagac
    11341 aattattgaa cctaatactg cagaagcata tccagacttt atgtgggcag agaaaaacca
    11401 cattaccgtt aaacattatg aatataatcc aaaagaaatt tttggcaagg aaatagacta
    11461 taaagaagat acaaatccta aagtttatgt aactggaaag aaaaatgttt tagttgctaa
    11521 caccaatgca agcggatatc atgatggtga tgatgaccaa ccagctttct tcagaaatga
    11581 tgcaaatgct ggcggaaaag cgatgaaagc atggggttac ttagtacttg gaccagaagt
    11641 aaataaaaag caagcaattg tattaggaat tgaagctaat actggtgctt atggatatat
    11701 agaagttggt ggtaaaaaga tagtatttgc cgatggtaaa aacaatggca agaacgttat
    11761 atctaaagaa gtatctgtag aattagaaag aggaaagatt tatccaattt atatggaatg
    11821 gtatgcagaa aatggaacag caggcacctt tgtacctgtt tataagacca ataattctga
    11881 atggagacaa attcctcagc aatggttcta tgcaagttcc aacgaaggac ctggacaaaa
    11941 gggacaacaa tttgaaacat ctgcattgta taaagaagta gctttcccaa aagaaaacgg
    12001 atactactat gttgcagttc gtgctaatac agcatttgct ccagaagcaa cacatggttt
    12061 atatggacca ttcttaattg gagatgctga agttgaagaa gaatacacaa tcgaattagt
    12121 tccaacacaa ttgttcttaa atgttgatca agttggtaca gttacagcaa agatcacacc
    12181 agaaggatat caaggagaaa ttaaatggac gattactaac aataaagatg cgaagaaaga
    12241 tgttatcgaa tttactggta acagcacaga tcttgttaaa tatgtaaaag gtcttcaacc
    12301 tggagaagca accattcgag ctacattaga taataatgca actgcatctt gtcaagtaat
    12361 cgtactggaa aatgttaaag gaagtatttc cttaccaaat tatctgtaca ttaaagaagg
    12421 tacaacaaaa gacttagcag ctatagttaa tcttggtgat aacgaaagtg ttaaatggga
    12481 aattaacgga aaagttatca acaataataa gtacaacgat gaatatatca aagtaggaag
    12541 cagtaagaag aatgtattga ccattaaggg tgaaaaggca gctgaagacg tatataacat
    12601 aattgcaaga tctgctgacg gaaaaattga aagtaatgta tgtaaggtat acgtatatag
    12661 taatcttaat caacatagct atttcgttgg tgaatctata agtcttgaag aattgttccc
    12721 aggattgcca acagatagac ctgatgaagt tgaatttaaa gtagctcttg gtaatgataa
    12781 agtaaaaatt aatggaggca atgtagatgc agttaaagaa ggtactgcaa aagtaacagc
    12841 aacgattaag ccaagtgaag acgcagaaaa tcaagaacca gaagtaatag aaaccaattt
    12901 aagagtattt acagtagtat tcaaaaatgt tactggtcaa gtattcccca acagagctac
    12961 aactacactt gtatttgaag taactgacgg agaagcagga tatgtaaatg taggcagaga
    13021 tctgaaggta aaagttaacc tgaacgatct taaagatatt gcaacagctt atgcgattaa
    13081 taatatccaa aacaatggtc aagcacaaga tattgtaatc gactatgata ctaagacatt
    13141 tactatatcc gtcgcatctg aaggtaataa agaacttgtt atattcatgg atcttacacc
    13201 aaatcctgat atgaaagtag cagaatacaa tcaattggtt aaagatgacg aaaaagttcc
    13261 agcaacgatt tctgttactc cggttggcgg acctacagta acagaagtag atgaagactt
    13321 agatattgca tactcaacta tcattagata atccaagtaa caaggcctga ccggttactc
    13381 ctttaccggt caggtctttt ttatctttaa gacttcaaaa aatgtgctat aatagtaaaa
    13441 aaaaaaggag atagcttatg aaaaaaatag gaattattgg cgcaatggaa gaggaagtca
    13501 taataatcag agacaaaatg gataataaag aagcctataa aattgcagga atggattttt
    13561 ataaaggatt tttgaataat acagaattgg ttattgtcca atgtggtata ggaaaggtca
    13621 atgcagctgt ttgtacccag gcactaatcg atcactttaa tgtggattat ataattaaca
    13681 caggggtagc tggaggaata tacagccaac ttgaaatagg agatatcgtt atatccgaag
    13741 acgctgtaca gcatgatttt gatacaagag cttttggtta tcctttagga gtaataccga
    13801 gaatggaaga aagttttttc agggctgatg accatctggt tcagcttgcc caccagagct
    13861 caaaaaatct gaatttaaaa gctaatgttt atattggtcg cattgccagt ggggatcagt
    13921 ttatttcgga tgtaaatgaa aaccgaagaa tttgggaaaa ttttaatgca tactgtacag
    13981 aaatggaagg tgccgcaatt gctcaaacct gctatctgaa taaaataccc tttgtgatta
    14041 tcagggctat atcggacaag gctgaccaca gtgcagaggt caattttcag gaatttgtca
    14101 atgaagcagc taaaaactcc agtgatttaa ttatagaaat tctcaataac atttaaaaac
    14161 tccaatattt tggagttttt ttcattttga agccaaaata agttttttaa gtgcaaaatt
    14221 tatgcgaaat tttgcacttt tttcttatat ggaattattt gacattttag cctcagacct
    14281 tataataaaa agtagttgta aaataatggg aggggttaaa atgaattttg catttttagt
    14341 tgaattaatt ccaatattta tgtatttaac agccatttcc ttcgggctag gaatgctaag
    14401 tttaatgttt ctgggcatta ggattagata cttaaaaaaa gcactgaaaa aatggacaaa
    14461 agaaaataag cggatttcaa atgatccaac cctggataaa atcgtaaaaa tataccgcat
    14521 gtacaatgaa caaggttgtg aaaaaataaa tacatcagcc attattcacg ggaattacta
    14581 taagcagaag attttattta taccgattta ctattgggaa cagtttatat cgaagtcaga
    14641 gatattatgc ctcttttttg ggctgattac cacatttata ttagtatcat gtcaaaagga
    14701 caacccgttt gaccccctga tttttgggac aggaactttt attattcttg taatcctcga
    14761 aatgttttta tctctggatg ccaaaaaaaa gtatctcttt gcacagatag aagaatacct
    14821 ggacaattct tacagataca gccttcaaag aactatggta acagcagaat attttgataa
    14881 agaaaaaata gaactgtttg atatgaaaga aggcaaggaa gaagaaatta aagatgaaga
    14941 aattgaatgg gttattaaac aattttatga tgtagaaaat gaggtataaa tatgcaattt
    15001 actgataata ctagggagag aattaaaaaa cattcttggc tgtaccatga aggaggagac
    15061 aaatggaaga tcctaaagtc aaattgccca ttaccaatga gtatggtttc tatgaaatca
    15121 gactggagag tatagggggt ttaggtgcca acttaagcgg gaagatttta ggggaattgg
    15181 gagccattta tcttggactg aacagttcaa gtttttcaag ttatggctct gaaaaaagag
    15241 gaacacctgt gaaagcgtat atccgctggg cagatcccag cagagaaatt aatatcaaca
    15301 gtccggtgga acgacctcat cttttaggac ttttccatga aaggctggct ggtaagattc
    15361 ctgtcatggc aggggttggc ccggaaacaa tcgtagtcgt aaataccgat gcaagtcctg
    15421 accagatgag ggacaggctt aaaatgcatg caggaacact tatctgtgtg gatgccttaa
    15481 agattgctat ggaagccaaa acaagagtca atatggtgat gttgggagca atggcgaagg
    15541 catccggttt tattccactt gaggctttaa agcagatagt cgaagatact ctgggtaaaa
    15601 aatatccggc agctcttaaa gggaatttgg aaggtattga aaaaggttat gaaaatattc
    15661 aaatgaaaaa atttgaggat gacggtaaat atccttatca ggaatacaag gaagtaaaaa
    15721 gagaatgggg atatgacaat gcgccaatag gcggtattaa tcctcatttt ggaagtacta
    15781 taagcaatga tttatccgga agcagggaag gatatatacc tgtttttaaa aaggaagcct
    15841 gcattaattg tggaatgtgt gacagcactt gtcctgatat ggtctatcaa tttgttttag
    15901 gtgagtataa aggcaagacc tctatggtca atttaggtcc tgattatcac cactgtaaag
    15961 gatgtctgcg ttgcgtggaa gtatgtccga cagatgccct gacagcaggt atagaaaggg
    16021 aagtagatat atggaagatt cacgtaagaa atcaggagct tatcgtagat aaattagaat
    16081 ttgaggatgt cgggccaaat tcatggatgc gttcagaatc tatcgtaaca aataaattgc
    16141 attaaggagg cacaccaatg gttgaacaga aagttgtttt tgaaagcgga aatgagctgg
    16201 cgtcctatgc agcgaaacaa ataaactacc atataatggg ctattatccc atcacacctt
    16261 ctacagaaat tgcagaatat ttagatctta tgaaatcaga aggagaacat gatattgtaa
    16321 tgattcctgc agacggagaa cacggagcag caggaatttg ttatggcgca tcaacagggg
    16381 gaggtagggt ttttaatgct acatctgcta atggcttaat gtatgccctg gaacaattac
    16441 cagtgcaatc agggactagg tttcctatgg ttttaaatgt tgcctgccgg acaatatcag
    16501 ggcccttatc gataaaaggg gaccacagcg atattatgta tgcgcttaat accggctgga
    16561 ttatactatt tgccaaggat ccccaaagag tatacgattt taatctatgc ggattaaaaa
    16621 tagcagaaaa agttaagctt cctataatca tcgcatttga tggctttttc accagccacc
    16681 agaaaagaag agcccaaatt attgcaaagg atgaagatgt tcaaaatttt attggtaagc
    16741 ccaatccgga gtatcatacc cttgataaag aaaatccgat tattgtagga agttatatga
    16801 atgaaccaga cttattaaac aacaaatatc aactgcatct ggctatggaa gaagcaagaa
    16861 aagtcattcc tgaaattttt gcagaatatc aggagctgac aggcagggct tatcagatgg
    16921 cagaaggcta taggatggaa gatgcggaag cggctttgtt tattttaggc tccagttatg
    16981 atacggccag aatggcagtg gacaagttaa gaaaagaagg gaaaaaagtt ggtgtattta
    17041 cttctaatgt ccttcgccct ttccctaaaa aagaactcta tgaaatatgt aaaaatgtaa
    17101 aatctattat tgtcgcagac aggcaagaca gttatggggc tgaaggggga aacatgactc
    17161 tggaattaaa agctgctttg caggattata aagccaatat cgaagttgcc agcttagttt
    17221 atggcttaag cggaagggat ttctatattg aagaaggtat agaaatgcta aacagggctt
    17281 atgaaacagc ccaaaaaggc aaagtagaaa aaagatttga ctactttggt cattacaaag
    17341 gcaagcaaga ttataaaatt gaaaggtatt ttgaaccgat tacaggtgaa aaagcaagac
    17401 cagggattac ttctgtagaa ttcgatgaag caacaggaag gtttaaagta aaaggcggtc
    17461 aggtaaagga ttcaaccaga atgccaaaaa gatggacggc tggtcatgga gcttgtcctg
    17521 gttgtggaat tcctgtcaat gtcaatatgc tgctcagagg tatcgaagga gaagtagttc
    17581 ttttattcca aactggctgt ggctatgtgg ttagttcacc ttatccaaaa agtgctttta
    17641 gggtaacttt cattcataac ttatttcaaa acggggcagc gactttatca ggcctggtag
    17701 aaatgtttca tgaaaagaag aggcggggag aattgcctca gggcgacaat ttcactttta
    17761 tcatggtaac aggggacgga ggtcttgata taggaatggg tgcagcgata ggggcagcct
    17821 taagaaacca taaaatgatt attatggaat atgataatgg aggctatatg aatacaggtt
    17881 atcaattatc ctatacaacc cctatgggag ctaagagttc aacttctcac gtcggtcctg
    17941 ctcaaaaagg gaaattaact ttccataaag atacaccaca aatctttgct gcaactaaca
    18001 ttccttatgt agctactgta tcagaaagcc aacctacaga ttttatcaaa aaggcagcca
    18061 aagcacaaaa atatgctaat gaatacggct tagcgtttat taaagctctt tctgcatgtc
    18121 ctttgaactg gaatgataac ccccgctatg aaagaagtgt catagatgca gcggtaaact
    18181 gctgttatca tcccttatat gaaatagaag aaggaataac cacaatcacc tataatccgg
    18241 aagaacggaa taaaaagata ccagttattg actggttaaa gatgatggga agaaccaaac
    18301 atctggttaa gccagagtat gcggatattg tagaaagcat acaaaaagaa gtggatagaa
    18361 ggtgggaaag actaaaagca agacatgcca atcccctttt gtaaaccaac tcttaagagt
    18421 tggtttttca atttatgtag aaaaaacgaa aaaaacgtga tattatgaca tcataggaga
    18481 ctagagagga tgatatcatg aaaaaattat ttatgatgtt ttttattttt atggttatta
    18541 cggttttttt gaccgcttgt gattcaaaag atattgctat ggaagatgaa gacaagcagg
    18601 ttatagaaaa ttctcaggaa gaaatacaaa atgaagacac tgaaatggaa gaagcaaatg
    18661 aagaagaact ggaagaaaca atagaagaaa aacaagaaga agatgcagag cccaaaaatg
    18721 aagatataga gaatcctgtc aataaagacg ataaagggaa tacaggcaat caggacaaga
    18781 aagaagaagt ttctaaaaat gagcctggaa ctaccacaaa gctttctaat gacttaaagt
    18841 cctggtggtt taaaagaaat aatgaacatc ttcctccagg ggcccagtcg gaaattgatc
    18901 tttctaaata tgatgcttat tatttaggca atatcagtga aaaaaagatt tacctgactt
    18961 ttgatgaagg ttatgaaaat ggctatacca gtaagatatt agatatttta aaagaaaaca
    19021 atgtaaaagc ggccttcttt gttacaaagt catatataaa atctcagcca gaactgataa
    19081 aaagaatggt cgatgaaggt catgtggtag gtaaccattc ggttacccat cccagtcttc
    19141 ccagtaaaaa cgatgaagaa gtaaaatatg aaattacgga aacagccagg tattttgaag
    19201 aaataaccgg gaaagacatg gatcctttct ttagacctcc tgcaggagaa tatagtgaaa
    19261 gaactcttca aattacgaaa gatctaggat ataagaccat cttttggagt atggcttata
    19321 aagactggga cgtcaatgat cagccaggga aagaagctgc ccttaaacac gtggttgaca
    19381 accatcatcc tggtgctatt attttactcc atgcggtatc gtcatccaat acagaagctt
    19441 taaatgatat gattcaatat cttaaagcac aaggttatga ctttgcctcc ttatatgaaa
    19501 ttaattaaaa ttaaattaaa tatttaattt taagacaagt cgtggtatac ttttaatgat
    19561 ttttgtagag aatcattaaa aataaggagg atacagatgg gaaaaatatt atttgattat
    19621 tcaaacgccg gcattaagga tgaggaaata aaaaatgccc tctggcaatt aaaagatgct
    19681 cataatatgc ttcacaataa aactggagca ggaaatgatt ttttaggttg gattgactta
    19741 cctgtaaatt atgataaaga agaatttaat cgggtaaaag cagcagccaa aagaatcagg
    19801 gaaaattcag atgtatttat tgtaataggc attgggggtt cttatctggg agccagagcc
    19861 gttattgaag ctttaggtca ctctttctat aacatgctgc caaaggaaaa aagacaagga
    19921 cctgaaattt attttgtagg taataatatt agtggtactt atgtgcaaca tctgttagat
    19981 ttaattgaag gaaaagatat tagcgttaat gttatttcta aatctggaac gacaacagag
    20041 ccaggtattg cttttagaat ctttaagaaa tatgttgaag aaaaatatgg caaagaagaa
    20101 gcgaaaaaaa gaatttttgc aacaacagac aaggaaaagg gagctcttcg taccttagcc
    20161 aatgaagagg gttatgaaac ctttattatt ccagatgatg tcggaggccg ttttagtgta
    20221 ttaacgcctg taggtcttct tccaattgct gtagcaggta ttgatatcga caagttaatg
    20281 gaaggggcta aggatggaag agaagaatat caaaatgagg attttgaaag caatattgct
    20341 tatcaatatg ccattgtgag aaatatttta tatagaaaag gcaaacaaat tgaaatttta
    20401 gccaattacg agccttctct ccactttatc tctgaatggt ggaaacagct ttatggtgaa
    20461 agtgaaggta aagaccaaaa gggaatcttt ccggcttctg tggatttttc aacggacctt
    20521 cactctttag gtcaatatgt acaggatgga agcagaatat tatttgaaac cgttttaaat
    20581 gttgaaaaac caaaacttga tattacttta gaagcagaag agacagattt agacggtctt
    20641 aattacctgg caggtaaaac catgagtttt gttaataata atgcattcaa aggaacctta
    20701 ttggctcatg tagatggtgg agttccaaat cttgttataa cgattcctga aattaatgaa
    20761 tactatattg gaaagcttct ttatttcttt gaaaaagctt gtggtatcag tggttatttg
    20821 ctgggagtta atccttttga ccaaccaggt gttgaagctt ataagaaaaa tatgtttgcg
    20881 cttcttggta aacctggata tgaagaatta agaaatgaat tgcaaaagag gattaaataa
    20941 ctttttgttt agatatgtaa ggtggtagca tatggataag gaacaaagaa aaaacatcat
    21001 actggccatg ctaaaagagt ctaataaacc tcttgcaggc aagtatctgg cagaaaaatt
    21061 aggcgttacc agacaaatta ttgttcaaga tattgcactt ttgagggcag agaaccataa
    21121 tattatgtct actgccagag gctatgtttt ttatgacaga aaacagtatg caaataaaag
    21181 agtgattact gtttcccata aaaaagaagc tatagaagat gaattaataa caattattga
    21241 tttaggaggc acggtactgg atgtcattgt agaacatcct atctatggtg aaataactgg
    21301 cagtttgatg atagaatcca gaagggacat taaagaattt atggaaatta tgaaaaagaa
    21361 tgagactgtt cccttactaa cccttaccag taatattcat atgcatacca tagaagctaa
    21421 aaatgaggaa atattagatg aaatagaata tgaattaaaa agaaaaggct atttattaat
    21481 agatcattaa cacccgtttg ggtgtatttt tttacataaa aatacagaca cattatttta
    21541 cttattgagt tgtcacttta aaaatgttat aatacattta cataacttaa tgaaggaagg
    21601 tgaaagaggg tgacagagaa tgaaaaaaga atcatttctt taaaacgggc agaggaatta
    21661 aaaagccgta ttaaagatta tacgatgaaa aagaattcta taaaaacagg ttttacccat
    21721 tcagaaatga taaaagaaaa taaaagacgt attatgaaag tgctaaatgc gtcagaaaag
    21781 gaatgggatg attggaaatg gcaattatcc aatcggataa cagatgttca tacattagct
    21841 aagattctaa atttatcaga tgaagaaatc catcaaatag acatgatagg gaaaaagttc
    21901 cgatgggcca tttcacctta ttatgcaagt ttaatggatc ccgatgaccc gttttgccct
    21961 gtcagaagac aagcggtacc ttctattgaa gagatggttg aagcaggaga agaagatccg
    22021 atggcagaag aatttacttc tccggttgaa ggtattacaa gaaggtatcc ggacaggctc
    22081 attgttaaag tgaccaatca atgtgccatg tactgccgac attgtcaaag aagaaggtgt
    22141 atagggcaaa aagatttggc cttgcctatg gctgccctta gaaagtgtgt ggaatatata
    22201 agaaatgaag aagaaatccg tgacgtactt attactggag gcgatgccct gttaatttct
    22261 caaaaaaatt tagaatggct cttatcagaa ctgcatgaga ttcctcatgt tgaaattatt
    22321 agaattggaa caagaacgcc tgtaacgatg cctcaaagaa taaccaagga gctttgtgat
    22381 gtgttaagca agtatcctcc aatatactta aacactcatt ttaatcatcc tatggaaatc
    22441 actgaagacg ccaaaaaagc aactgacatg ttgtgttctg ccggggttgt tctgggaaat
    22501 caggccgtat tgttaaaggg tattaatgat gatgtgcata ttatgaaaaa attaaatcag
    22561 gagttgttaa aaatcagaat aagaccttat tacattttcc atcccaaaga agttgtagga
    22621 acagctcact ttaaagtaaa ggttcaaaag ggaatggaga ttatggaaaa attacgaggc
    22681 tatacttctg gtctggctat tccgacctat atcattaatg ctcccaatgg aaaaggaaaa
    22741 acacccattt tgcctaatta tattttatcc tgggaagaaa ataaaattaa acttcgcaat
    22801 tgggaaggag aagtctttta ttatcctaat taattaattt attggaattg aaatatttca
    22861 taaaactata gttaattcct gcagaaataa tatcagccat tttcatgaca agagataagc
    22921 gggtattttg taaaaccaac atatccataa aaccactgaa attaacaatt cccgtaataa
    22981 ataaatctcc aacagggggt aaatctttcc ttactcctgc ccccggcttt aaggcccctt
    23041 ctcctatcgt tatataccct acatgttcca tttttcctaa acaggcatca atagcaatta
    23101 ttaaaggatc cagatggttt ttgctgatca tatccagggt ttcctgaaga tttttggcat
    23161 gaaccggttc atccaggctt ccataaacaa agacattggg ataagatata tttttcagtt
    23221 tatagccaat caaagggcct aagctatctc ctgttgccct gtcggttcca atacacaaaa
    23281 taatggtttc tttccggtaa ggaatttttt tagagaaata ttccagaaag caattgctaa
    23341 attctttcag acacagattg cttttcgtat caatataagt tggcgattct atttttttat
    23401 gagcaaacac tacgttaata ccacctttag gaatagttat ttttagtatt cctatttttt
    23461 ttgtaattat ttctaaaggg tagtggaatt ttttggtgaa aaaccatgat aaaaaataag
    23521 aatccctcga cacatattac caaaaaaagt aaaaagtatg tggtattata ttttcaaaca
    23581 ggtaatatat gcacgagggg gatgaaatat gagtgaattt cttaatgact cttttgattc
    23641 cggtgatgaa ttaaaagagg atataacaga tacctctcct tttcttgaaa atgcaaaatt
    23701 aattggtgaa agtccgacag ggttaaaaat ttatttagag gattacgttt acacttacct
    23761 gtacaaatat gctaagtttt accggggaga ggaaagggca gcggctttga tagggacttt
    23821 tatggaagat tcgggacaaa tgtgtgcagt aataagcgga gcagtacaaa taccgccaga
    23881 gacaaaagga gaaacgggag agctttttct agaaacgcat cttgaagaaa ttcaaaagca
    23941 aatggaaaag tacttcccgg acaaagaact gatgggttgg atgcataccc agccaggata
    24001 cggtattttt ttgacgacgc aggatttgct tattcaaaag aaatatttta aagaagcttt
    24061 tcaaaccctt atgattatag accccatcga agacatggat gctttttttg catgggaagg
    24121 agaagaatta agatctgtaa agggatatta tctttattat cataaaaatg aacagatgca
    24181 aaattatatg attgccaaca aaataggact ttccagtgaa gagttaaatg accgggaaga
    24241 tgtcgtcatg cactttagaa aaaaagacag gatgagaaga caggaaatac aaaacaagaa
    24301 aattatgcgg agggtgacga ctttaagctg tgtattattt ttaatgtgtg ctacaatggc
    24361 tgtcaattta atacaatata aagagcgttt agccaggtta gaaacagaat tagaccatat
    24421 aggcagtaat tattcagata tccttaagcg tttagataaa gaaaatcttc aagttgtttt
    24481 tgcagaagaa aacattgaag atattataac tgaaacggaa gaatatgcgg ttatcagtgc
    24541 agaagccaca gaaatagata aaggcaatga ggacaataat aaaaatgaag aaaatacagc
    24601 taatgcagat catgaaaata gagaagctaa taatgaaaca aaagaagaaa cagcagagcc
    24661 tgaagccgtt gattactata cagtccaaaa aggagacaac ctcatcaaga tatgttatca
    24721 acattataag acaaccaata tgttaggaaa gattgttgaa atgaataatc ttgaaagtcc
    24781 taataaaatc tatgaggggc aagttttaaa actccctcgt ttacaataaa gagcagcccg
    24841 cagaggctgc tttttactgc attttttcca tttccataag aagattttta aataaatcca
    24901 gggctttttt aacaggttcc gggctggtca tatccacgcc agcttttttc aggatattaa
    24961 taggataatc agaacttccg ctttttaaaa atgttatata gttttctaaa gcattttctt
    25021 cattgtttaa gattttattt gacaaggcaa tggcagcaga atagcctgta gcatatttat
    25081 acacataaaa ggcattataa aaatgaggaa ttcttgccca ttcataatta atttcaggat
    25141 caacaacaat gtctggtcca taatatttta tatttaagtc cctatagata aaacaaagac
    25201 tgtcggaggt taaagcttct cctttttcta ccatttcatg ggtgattttt tcaaactctg
    25261 caaacatcgt ttgacggaaa agggttcctc taaactgctc cataaaatga ttgattaaat
    25321 aagctctttt tttctgatca tttgtcgtgt ttagcagatg ttgcatcaac aaagattcat
    25381 tgaccgtaga agcaacttct gctaaaaaga tgggatactg ggcataaaca taagcttggg
    25441 tgttatcaga ataataggaa tgaatagcat gccccatttc atgagctaag gtaaagacgc
    25501 tatttatgtt atcctgataa tttaaaagaa tatacggatg ggttccgtaa gcaccccagg
    25561 aatagacacc acttcgctta ccttcatttt cgtatacatc aatccatctc tcgttaaaag
    25621 ccttatccac gattgaaata tattcttttc cgaggacctt taagccttct ttaacgatat
    25681 cctttgcctc ttcataagaa acttttatat ccatatcttt gatgattgga acatataagt
    25741 catacatatg caactggtca agaccgagca ttctttttct gagggcaacg tactggtgca
    25801 tgcagggcag atattcatgt accgtttgaa ttagattatc ataaacttcc acaggaatat
    25861 tatctgtaaa gagtgcactt tctaaagagg aagaaaattt tcttgcagaa gcaaagaaga
    25921 catctttttt cacacttccg ttataaatgg ctgcaagggt atttttttgt ttaatataac
    25981 tggaatataa agacttaaat gctgccttgc ggatttctct gttggggttt tccaataatt
    26041 gaatatatct tcctttggtc acttcaattt cttcgttgct ttcaccttta atagaaggaa
    26101 aacggatatc ggcatcattg agcattgtaa atacagattg agggatattt cctatttctg
    26161 aggcttttgc caggagcaat tcttcttttt ttgaaagagt atgttctttt tgtcgtaata
    26221 aatcattcaa aaagtgagta tatagtttta aatcctcatt ttcttttaaa taatttttta
    26281 aagattcttc aggacttgct aaaatttctg gaacaataaa agcggaaact gaagaaaagt
    26341 ttacggacag ggctttggct ttatccgcca gggcctgata agtattgttg gaagtatctt
    26401 cgtgaagctt catgtgagta tacacataga ttttttcaac catttgagaa agctgatcat
    26461 ataaggacaa acaagcatac aaattttctg aagaaagtgt aagctggtcc tgaaattctg
    26521 ataattgact catggaagcc gtaagctttt ccatgtcttt tttacaagct tcatcgtcag
    26581 gatataagtc ttcaagtttc caggtatatt gtggattaat ttcacttctt ttaggcagag
    26641 catttcgtct attttttccc atataaaaac tcctttttat tttttattat accctttcta
    26701 taagagaaga taaacaaatg aaataatttt gtcaatttta ccaagaaaaa taaaaaagca
    26761 gttgacgaga gatgaaagaa atgatattat taattttgtg gataagggtg tggataatgt
    26821 gtgtataaca tgtgcataac ttttgttgtc cacagggtgt taaaagatta ttttagaatc
    26881 ttttataatc gcgtaaaagc gacattatgt cgctttataa taaaatatta aataaacctc
    26941 gtaaagataa gaaatccccg tttcttatca gcaaccaccg gaaccccaac cggtgctttt
    27001 ttttataaaa atataaagaa tctaaaaacg tatatttctc atcctaatgt taagaataaa
    27061 agtaagcaga aatcattagg gtggtgaaat aatgacggtt ttagaaacga tagaaaaata
    27121 tatatctgat ttttcatccc tggagaacgc agaagaaaga tattgcattt ctttattata
    27181 tgattattgc aaatataaag aacctcaact ggaaattgaa gaattggatg aacagttctt
    27241 tgataaattt tttctctact ggctccctgg aagaaataaa atgcttactg gggacaggct
    27301 ttattatctt tttcctacta ttaaaaaata ttataagtat ctaagaaaaa attataaaat
    27361 taaagaatta aatttaatta aatcc
//
LOCUS       DAOSUD010000022        27151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1170_length_27151_cov_22.230071, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000022 DAOSUD010000000
VERSION     DAOSUD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 27151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27151
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_27151_cov_22.230071"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1583
                     /gene="amrA"
                     /locus_tag="PLG49_04485"
     CDS             192..1583
                     /gene="amrA"
                     /locus_tag="PLG49_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOQ16611.1"
                     /translation="MEGFYLLPHPPIIVPEIGKGEENKIEASSKSFEIIGQEIKKKAP
                     NTIILITPHGPMFNDAINITYENSIKGNFGQFGASQISMEIRINKKLSKEISETAKNS
                     NIPVVLSTKELLSHYNVHISLDHGAMIPLYFINRFYMDYSLVHITYAPLGDLELYQFG
                     IAIKKAVEKTNEKVVLIASGDLSHRLKADGPYGYNPSGEKFDDAFLRLLENGQVTEIF
                     TMNKEIIYNAGECGRKSVLIMLGAMDDIHFKGHLLSYEKTFGVGYGIMKFESTGPCQS
                     KLNTIVDAVKNKNNNQSNPYVRLARESLTTYLEKRKMMEELPSYVTDEMKNLKRGVFV
                     SLKKNGNLRGCIGTIFPATSCIADEIIRNAIEAGLYDPRFEAVELSELNDIEFSVDVL
                     TEPETATKEDLDPKEYGVIVRSKGRTGLLLPDLEGVDTVEEQLSIALRKAGISPFENY
                     SIERFKVIRHKEE"
     gene            1597..2451
                     /gene="amrS"
                     /locus_tag="PLG49_04490"
     CDS             1597..2451
                     /gene="amrS"
                     /locus_tag="PLG49_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOQ16612.1"
                     /translation="MKVEACFYEKHRDKIHCFLCPHHCLIENHEWGKCKVRIHEDGVL
                     YSINYGEITAAALDPIEKKPLHYYKPGTFILSVGSFGCNLTCSFCQNYSISQYKAQST
                     YIAKEKLALTSINMENNIGLAFTYNEPTIWYEYVYDTAKYLKENFPFYVIVLITNGYI
                     EEEALKKLLPYVDAMNIDLKSFNEQYYKELCGGRLKPVLKSIELAAKSCHLEITTLLV
                     SKENDNLTEIENIAKFISSIDSNIPLHLTRYFPNYKLNHPATDKEILYKSLEIARKYL
                     SYVNLGNI"
     gene            complement(2448..2957)
                     /locus_tag="PLG49_04495"
     CDS             complement(2448..2957)
                     /locus_tag="PLG49_04495"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ16613.1"
                     /translation="MTTKKLTITALMAALVTIVTMVIAIPIGLQGYLNFGDILIFSSA
                     LIFGPEVGFIAGGLGSALADLLSGYAAWIPITLLAKGLEGYIAGKMCYRTKSKKQYFL
                     GICLSGFVMVFTYYIGGVILVFSSEGFKAALSSALSAVPFNILQVISGGIGGSMIAFL
                     LKDRIEKVS"
     gene            complement(3087..3854)
                     /locus_tag="PLG49_04500"
     CDS             complement(3087..3854)
                     /locus_tag="PLG49_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ16614.1"
                     /translation="MIYEPYLYDDVFVLRNKFDIKDAKKLKEVENKIVSDRLHTLSKY
                     PIKGKFDFSHMREIHWFLFSPIYDWAGHLREEDIIRHELILNHRSIQYETCKNIEKSS
                     EMILNSMKAVPWNNLTFDQKISEISKYFAELWRIHPFREGNTRTISTFIIQYMEEQGV
                     YISRENFSKNAQMLRDALVYAADKDDSRQPEYLEAIIRWSIDKNNRESEKNRGTGNKS
                     LMDYLDIINIKRKINLIRRIDKNNENIKNKEDVDKIK"
     gene            complement(3974..5185)
                     /locus_tag="PLG49_04505"
     CDS             complement(3974..5185)
                     /locus_tag="PLG49_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ16615.1"
                     /translation="MNIFESLKSALRNLMSNKMRSFLTMLGIIIGIASVIMITSIGAG
                     SRAAITSEFDKLGVGLLTIQMKGSNDAKLRDFLTLDDVELIKKHPEVKNVTPYFEAWG
                     ASIKLKNPKEEKSGYIIGINEEYRFMVPPDLLYGRFIIESDCKAKSKVVVIDEVLAEK
                     VFGRKDVVGETITVRLWRGNLKFTVIGVTKNSNAALESIMGAEMPSALYMPIQTVMDI
                     YLVDYLDYIYVSVKDPEKIDQTAIELNKMLEMKHRNEDAYYIRSLMDEIEMINQVLGY
                     ITAFISFVAGISLFVGGIGVMNIMLVTVTERTREIGIRKSLGAKNKDIRKQFLIEAVI
                     LTVIGGIIGIIAGFLGGSLVGMLMDVTPKVSLFSILVTVGVSSLVGIIAGVYPANKAA
                     KLDPIEALRYE"
     gene            complement(5182..5889)
                     /locus_tag="PLG49_04510"
     CDS             complement(5182..5889)
                     /locus_tag="PLG49_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16616.1"
                     /translation="MIKVESLNKIYENGRIQVAALKNINLTVSQGEFVAIMGASGSGK
                     STLMNILGCLDRANSGKYFLEQVDVSSLNDDELAKIRNLKIGFVFQAFNLLPKLTALE
                     NVELPMIYAGAKHKERREKALAALDKVGLSDRVLHLPNELSGGQKQRVAIARALVNSP
                     SILLADEPTGNLDSKSSEEIMGIFQKLNDEGVTIVMVTHEPEIAHHTKRIVTFKDGEI
                     ITDKFVDNPIRVGGDAL"
     gene            complement(5891..7345)
                     /locus_tag="PLG49_04515"
     CDS             complement(5891..7345)
                     /locus_tag="PLG49_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ16617.1"
                     /translation="MKNMSVKNKKLFIGIGIAACLLIFIIAAAINTSSSVTASGAPKG
                     AISVAVEEARKDSIISTVNVSGVVEEKDSEIVYVNSSIKIDDILVEPGDMVKKGQKII
                     EYDSNSLAEIEIQLKEAEIALQNAELTLSALTGADKGEILNAESAYTMAKKALNDAET
                     SLKQAESNIEQLRQKVKDAEQMVANNKILFNQGAISKLELDQSINQLNELRSQLEAEE
                     MRIASEKLNIENARKQKALAEYNLSTTYNKYADSKKENEIKIQQNQVEAARMRVEALR
                     LKRDELLASMESPMDGTVVEVFAESGTWVAQNSPVLKIANLDKLIVRVQVSEYDAPSI
                     KPGQKVTFSGDAIKKEKIEGKVIRVSPVATNINLGSSQEVMVEVEIEVTKNSANLKPG
                     YSVDADIITAEINDAVVIPLLAVFEENDENFVYIVKEDYTVEKRKIEVGAYSDLYVEV
                     KGVKEGEKVITNITAQVQEGIKVKPIENKVESGE"
     gene            complement(7370..8104)
                     /locus_tag="PLG49_04520"
     CDS             complement(7370..8104)
                     /locus_tag="PLG49_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOQ16618.1"
                     /translation="MENIAIKQEVEVTASKPMGIFKKILQIFISPSEVLNDVMEHPGL
                     LMPLLYSSILMFLKQLITIPVQRINLQKLNDIYAQYGMPVQTEISTAQSVISAVLSPV
                     FLILSWLFVSFVLWVLVKVFKGKSSFKQMLALYAHALIILVTGSLIAAPFNLILQTDI
                     VIFSPVAFIPNPDITSFIYLFFSNLEFFTIWTMIIVAMGISLFNNFSKKKGFIIAFLY
                     FFSAKLLTALYGYFSFKLLSNLSSFM"
     gene            complement(8255..9370)
                     /locus_tag="PLG49_04525"
     CDS             complement(8255..9370)
                     /locus_tag="PLG49_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ16619.1"
                     /translation="MYDDRIKKLARTLVRYSVNLQKGEKVLIQGNGLEVSLVNAIIKE
                     VYEVGGYPFVQLNDQRILRELYMNTTKEHMEAMAKYEADRMKDMDAFIGFTSIRNASE
                     LSDVPSEKMDLYYKYFWKTVHVDIRVPKTKWVVLRYPSPAMAQMADMSTEAFEDFYFN
                     VCNLDYAKMSKAMDVLVDLMEKMDKVRIVGKGTDLTFSIKGLPAIKCDGKMNIPDGEV
                     YTAPVRESVNGIISYNTPSIYHGFTFENISFEFKNGKIIRATANDTERINKILDTDEG
                     ARYIGEFAIGLNPYITKPMKETLFDEKIAGSIHFTPGNAYDDCFNGNRSAVHWDLVLI
                     QRPEYGGGEIYFDDILIRKDGLFVLPELKVLNPDYLK"
     gene            complement(10323..11357)
                     /locus_tag="PLG49_04530"
     CDS             complement(10323..11357)
                     /locus_tag="PLG49_04530"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ16620.1"
                     /translation="MNKICFFDTKPYDKMYFDLLKEAYGIEIDYFEPKLGPRTAFMAN
                     GYEAVVAFVNDTINKETIETLYQNGTRLIAMRCAGYNNVDFKAAYGKIHVVRVPAYSP
                     YAVAEHAMALLLALNRKIHKAYIRTRDFNFSLNGLTGFDLHGKTVGVIGTGKIGRVFM
                     DICKGFGMKVIAYDPYPLKDSDIHYVSLEELFKESDIISLHCPLTKDTFHLINEETIK
                     MMKKGVYIVNTSRGALIDSEALLEGLKNEKIGGACLDVYEEESELFYEDMSYVVIQDD
                     VLARILSMPNVIVTSHQAFLTKEALHNIAETTLKNCRDYFDDKVLENEVCYRCGRGEV
                     CLRKENKRCF"
     gene            complement(11404..12954)
                     /locus_tag="PLG49_04535"
     CDS             complement(11404..12954)
                     /locus_tag="PLG49_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOQ16621.1"
                     /translation="MSRRYLFFILVFFISIFMAGFTNYNEADEKNELTIMIVPLDDRP
                     VNTDFPKAIADIAKVDILLPPNEILGGRHKKADIESIKQWALENSKYADAFIISLTMT
                     NHGGLIHSRMLDNDNFVIDLDWLRELNDKNPGKEIYAFDTIQRLSVSVDNKYEKELYD
                     NIQNWAKNYMITDRNKLKALEEKIPGELKNKYLYSRAVNLSFNLEAINLVQEGVIDYL
                     VLSQDDAAEKGIHLLDQQQIKNKIHELGVEDKTSMIIGTDEVELNLLMRHILKHYNYS
                     PKVSVQYINENKKGIILSYEDRPLDKTIIEHIELAGASYVSYATNNADLYLFVYNSIE
                     NETITLDFIKKIKEYIESGKTVGIIDLSNNLSKSSANDFVSLLRKNINLSDLGSYSAW
                     NTASNASGISISHLLAYWHSKRFLLGTEQYHQKFLYNRFMNDYVYKVKIKPVLESYLI
                     STGSDVHNLDASYGKSYLESYITDHFIKLQSDFLMEFDDVYWDKIAIKKVELPWDRLF
                     EIRIVNYN"
     gene            complement(13418..13810)
                     /locus_tag="PLG49_04540"
     CDS             complement(13418..13810)
                     /locus_tag="PLG49_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ16622.1"
                     /translation="MRVEHIAIWTNNLERLKEFYVTYFDAQANQKYINASKNFESYFL
                     SFPDGECRIEIMKRPKLIKMDCNDEKNYIGLAHLAISVGSKEKVIDLTEKLRRDGYKV
                     LSEARTTGDGYFESVIEDPDGNRIEITI"
     gene            complement(13935..15035)
                     /locus_tag="PLG49_04545"
     CDS             complement(13935..15035)
                     /locus_tag="PLG49_04545"
                     /inference="COORDINATES: protein motif:HMM:NF027433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4825 domain-containing protein"
                     /protein_id="HOQ16623.1"
                     /translation="MVIPASASLSSDGITFAVYDSEEKTYKEYPEIETLAYKNHLAVR
                     PFANMVGLIEGAGREMSRNKKVKIFNISGGTVDITDEDFVAMTPSFTLDGKKILYSAA
                     DTKDFNDIIFNNKIPAFNIYEYDLKSSIIQRLTEGNHFDFLPMDLSNQQILFIRYKDN
                     GYFSLIKLSDGQEEIFADNIVFADAKESFGYYGHISTEMSLDIYISNRDQFNKNLKKE
                     NSEPDKVDELYELRGTYIGDNVRVGKIIRYLNFPEGLSSNGMELFTKKEPYGLQINFM
                     ADEGAKKKYMSMNSDYIWRTQSLILFSLIHNLDYIQYAIDDGNSIEIIFYTDRKDADA
                     LSMSLMSCKSSEIAESPESFRQYYNILNSNDF"
     gene            complement(15119..16714)
                     /locus_tag="PLG49_04550"
     CDS             complement(15119..16714)
                     /locus_tag="PLG49_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOQ16624.1"
                     /translation="MDFLNKIFQMSLTGSVIFLLYLIFKPLTKKLFNSSWHYKMLIVF
                     LCFFILPIDSYLQLPVRQFARIPKIRIEESIFPQSKNNKAERKNETNPINNESTANEV
                     HISEKEANYKERESNEFGLEDNTILNKKINLYPYKNLLLYFWLIGVIALFLLKIISYL
                     AFKRTMLKSSSFVKNDEVIQLFDKCKEELHINKKLELRIYKNLASPMLIGILHPIVLI
                     PETDLGHKGLELVFLHELNHYKRKDIIIKTVGLFAHAMHWFNPLIYLLLKEMNKYCEY
                     SVDEKVVENMDKNDRKFYGEMIINLISNSAFRNCSLTTAMSNGGRQLKDRLENMFYSL
                     KTTRKRQFMSFISGIIIVTCGFTVAYSLFADNQWKNEKSFVVYTKEDGLYYSYLDMQA
                     EIKIDDGDDFFCPLISKGGNYIAYTKNNSLYIYSLRDSSLEKVDDDIVHHFTSYDWID
                     DRSIVYGSQERYGFILYNVITKEKTPHLEDAYYTALVASDQKKVYASKSRSENGLMIY
                     EGIVEIDLNQYSKDKQQFLRIRL"
     gene            complement(16716..17084)
                     /locus_tag="PLG49_04555"
     CDS             complement(16716..17084)
                     /locus_tag="PLG49_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOQ16625.1"
                     /translation="MNIIKISDAEYEIMEIIWNAKREVTTADIMEKLEKNKSWKLTTV
                     LTLAKRLVDKNVLQVRKEKRVNYYSPKITKEEYKSYQASGLIQEMFNGSIKSMVASLY
                     DNKKIDNKDLEELKDWIRGL"
     gene            complement(17178..17912)
                     /locus_tag="PLG49_04560"
     CDS             complement(17178..17912)
                     /locus_tag="PLG49_04560"
                     /inference="COORDINATES: protein motif:HMM:NF020448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3658 domain-containing protein"
                     /protein_id="HOQ16626.1"
                     /translation="MEGDANNVVNIGFYLDIGDISGEIDGSERKKVLSQLWTRFNIED
                     NERELFFKYQRKDLEKLISAAKEGMDIRIWTSHAPYSVCGFYYVCYLLRNIDCKIRVV
                     SLPQFISFSNHEIIECSHWAEIKPGQFYQFLNLEKPLSSIEKQYYSQKWQDLVLENAP
                     LRAVINGKLTSVEESFYDFIIINNLPDNDFLMGQLIGKLLGKYKLGISDAWYALRIDK
                     MIEENKLKLVKIKDTSYPYGKLLRKI"
     gene            complement(17936..18061)
                     /locus_tag="PLG49_04565"
     CDS             complement(17936..18061)
                     /locus_tag="PLG49_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16627.1"
                     /translation="MLEVVFSDSEKASMKLAKCYNEKKFLADLLLILGANRLKQK"
     gene            complement(18223..19086)
                     /gene="nifH"
                     /locus_tag="PLG49_04570"
     CDS             complement(18223..19086)
                     /gene="nifH"
                     /locus_tag="PLG49_04570"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HOQ16628.1"
                     /translation="MEAMSKQIRQVAIYGKGGIGKSTTTQNLTAALAESGKKVMVVGC
                     DPKADSTRLLLGTLAQKTVLDTLRETGEDVELENIMRTGFGGIRCVESGGPEPGVGCA
                     GRGIITSIGLLEQLGAYTEDLDYVFYDVLGDVVCGGFAMPIREGKAQEIYIVASGEMM
                     ALYAANNIAKGIAKYAQKGGVRLGGIICNSRNVDREIDLLRAFCAELGTQLIHFVPRD
                     NVVQHAEINRKTVIEYKPNCHQANEYRQLAKAIDENDFFTIPKPMSQERLEEILLEYG
                     LMEALEDDYRI"
     gene            complement(19109..19912)
                     /locus_tag="PLG49_04575"
     CDS             complement(19109..19912)
                     /locus_tag="PLG49_04575"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16629.1"
                     /translation="MSETLSVISRTILRIENVSHTYYDDYGNHVEALRDINLSVVRGE
                     FISIIGSSGCGKTTLLRLIAGLEKPEKGTLILNGEKITEPDPQRGYVFQHGCLFQWLT
                     VEKNIATGLRARGIYRENKELVQHYIDLVGLRGFEKAYPHQLSGGMAQRVAIARALIN
                     KPKVLLLDEPMGALDSFSRADIQDKLLELHKENETTMILVTHDVDEAIYLSDRIVIMT
                     PRPGKISEIMDINLPHPRHRGGTDFLYLRRTILEKIHLVSALPEPEYII"
     gene            complement(19909..20976)
                     /locus_tag="PLG49_04580"
     CDS             complement(19909..20976)
                     /locus_tag="PLG49_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ16630.1"
                     /translation="MVELNKLQAQTIPIFKTKKDKIQLKNLSDKQWFFYFILTLLGFL
                     IAIAANKFIRQMANVEIKTYMIFGIPFTFNTFYRLLLGVLLLFYAIAGIFSYFNPSAR
                     VKFSSRAPFRFAVGLALAIWDILGTKYLLLPQPFFPGPAKILEPFLMEGDFIFSNMLY
                     SLKLFTVGFSLGVLLGIGTGIIIGWFPKAYYWLYPILKLTGVIPAVAWMPFALTLFPT
                     PFWAAVFLIVICGWFSIAALTAQGIKSTPKVQLEVARTLGASTFFLVFHVAVPHAMPQ
                     IFTGIANANGFAFTTLVMAEMMGQPGGLGYYINLSKVWSAYYKVFASIFVMAILFSLI
                     MKGIALVQAHVLRWQKGWVKE"
     gene            complement(21061..22140)
                     /locus_tag="PLG49_04585"
     CDS             complement(21061..22140)
                     /locus_tag="PLG49_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ16631.1"
                     /translation="MKKSVQKGIALMTLIVIFANLLSACSQSSGGESQTLKTDDVQTV
                     ETSSSEYKYGKIDIPGKDGALCAAPIYIAYEKGFFKAEGFDVNLISADSETRKIGLNN
                     GTIPIVNGDFQFFPSIENGVKVKVVDGLHDGCIKLIVPKDSPIQTVHDLKGKRISVDE
                     IGGTPHQVASVWLERNGISAKQEDGEVTFLPFADGNLAIEALNKGEVDVAALWDPFGS
                     VQEKTGNYRVILDITKDEPFAGKYCCFLYASEKLLNEEPEKIAALLRAYRAAQDWISK
                     NPEETVDIIIEGKYAQIEDRELALELIKSYKYPSYADREEEPIRVYDNVYYFAEQLNQ
                     IGYLKTDPESFTNNAYAEVDINLGK"
     gene            complement(22163..23476)
                     /locus_tag="PLG49_04590"
     CDS             complement(22163..23476)
                     /locus_tag="PLG49_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ16632.1"
                     /translation="MSNQQKSEYGFDTLRLKAGYQSNEHNYAVSVPIYQTTSFDFRDV
                     EHAKALFGLRELGNLYTRVGNPTVAVLEQRVAALDGASGAIALASGMAAISYTILNVA
                     EGGGRILTSPYLYGGSADSFKKVYPKFGIHFDIAKNIEKPKKLAEEIKPDTKAIYVES
                     ISNPSAVLLDIAEIAKVAHEHGIPLIVDNTVATPYLYNPIDHGADIVVYSATKGLTGH
                     GNVIAGLVLESGKFDWKKEKFPQFHEKLYTLRDINDQYRSFLEVFPEFPFTARIRFTY
                     LNYFGAALSPFDVYLVLLGLETLSERIAKQVSNAKILAEYLKSHKSVEWVRYPALEDS
                     PYHSLYKRDFPKGAGGILAFGFKGTNEQREAFLNSVRLFHYHVNIGDARSLIVNSPQT
                     THSELEAHEKEVADIPENLIRISAGLEDPKDLIADLEQAFEKAYV"
     gene            complement(23434..23556)
                     /locus_tag="PLG49_04595"
     CDS             complement(23434..23556)
                     /locus_tag="PLG49_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16633.1"
                     /translation="MCSDCDMSDMADMYQYQTIQLLGGVFVCQINKKVSMDLIP"
     gene            complement(23719..23902)
                     /locus_tag="PLG49_04600"
                     /pseudo
     CDS             complement(23719..23902)
                     /locus_tag="PLG49_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256744.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(24235..24681)
                     /locus_tag="PLG49_04605"
     CDS             complement(24235..24681)
                     /locus_tag="PLG49_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOQ16634.1"
                     /translation="MRISAKGRYAMAAVISMAQQYNTGENITVISISEKLGISKIYLE
                     QVFALLKKAEIVSSEKGAQGGYQLTRSPAKITALDVLEAVETSLFEQVQETVLEKAPD
                     IDEAMRLSVFEQLDNNIRQILSSITIEDLVKEAEKHKSNNVIMYYI"
     gene            complement(24912..25424)
                     /locus_tag="PLG49_04610"
     CDS             complement(24912..25424)
                     /locus_tag="PLG49_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ16635.1"
                     /translation="MDNYDVALAALLHDIGKLVQRTGVPINDSLKGQESVFYKNMGGY
                     FSYKHALYTVQFLEENLKMVLDENIINMAAKHHVPDNQLEKLIQEADCFSAGMDRRKM
                     DGNERESIKKGQYINERLYSIFENINNGKEYKKQNYHYNLIPLNATEDIFPVKEVNLV
                     LSKSKFLAYL"
     gene            complement(25428..25826)
                     /gene="csx20"
                     /locus_tag="PLG49_04615"
     CDS             complement(25428..25826)
                     /gene="csx20"
                     /locus_tag="PLG49_04615"
                     /inference="COORDINATES: protein motif:HMM:NF040559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx20"
                     /protein_id="HOQ16636.1"
                     /translation="MKLLALISHTLMEGQVLEAKNQLKVDSIVLMPEDIKSIWANISP
                     FGPMPKDQLNQMIAWIEKESDKGDYVLVQGDFGATYYVVDYCIKNSRIPIYSITQREI
                     VEHIIDGQVESRRIFKHVNFRKYLPYEKEV"
     gene            complement(25849..26493)
                     /gene="cas6"
                     /locus_tag="PLG49_04620"
     CDS             complement(25849..26493)
                     /gene="cas6"
                     /locus_tag="PLG49_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOQ16637.1"
                     /translation="MIFRVFLHEKGFEFKGRKFKLFTFSQLYGNYQIDKENKIIIFPE
                     SVKLTVSSVVDDFNQEFLKTCLSRKNLTLNNQLLEVQRIAVNDNSYRIKDALVKTLSP
                     ITVYSTVYLNGRKKTIYYFPEDSLFQKYIRDNLIKKAELIYGRGFDDAKFEIKAIQDK
                     TQMNILYYKQFVIKGSSGLFEIHGDEELIHAALNCGLGSKNSQGLGCIQLIRAI"
     gene            complement(26836..>27151)
                     /locus_tag="PLG49_04625"
     CDS             complement(26836..>27151)
                     /locus_tag="PLG49_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16638.1"
                     /translation="FIPEINSENGEVNQETRFYYHQNGDLLWGEYAGGDIIKGTLIGT
                     VAKDGRLNFTYQHINKNKEIRTGKCQSVPTVMENGKIRLVEKWQWTNGDYSEGSSVLI
                     EI"
BASE COUNT         8669 a         5291 c         3884 g         9307 t
ORIGIN      
        1 tattagtttt ttgttaatgg ctatttttat tctattttat tataacattt ttttaacaaa
       61 cataaatatt tattctgcta ttccattaac aagctaaatg aaaatttgcc ttttggccca
      121 taatatattt ataatgaact taataatgga tattttaaat gaaattaaga ataatccagg
      181 aggaatgaat catggaaggt ttttatcttt taccccaccc cccaattatt gtacctgaaa
      241 taggaaaagg cgaagaaaac aaaattgaag ccagcagcaa aagttttgaa attattggcc
      301 aggaaattaa aaagaaagct cccaatacga taattcttat taccccacat ggacctatgt
      361 tcaatgatgc cattaatata acttatgaaa acagtattaa aggaaatttt ggacaatttg
      421 gggcaagcca gatatctatg gaaatcagaa tcaacaaaaa gctcagcaaa gaaattagtg
      481 aaacagccaa aaacagcaat attcctgtcg tcttgtcaac aaaagaactt ctcagtcatt
      541 acaatgtaca tattagcctg gatcacggtg ccatgattcc tctgtacttt attaatcgtt
      601 tttatatgga ttattcctta gttcacatca cttatgcccc cttaggtgat ctggaattat
      661 atcaatttgg cattgccata aaaaaagcag tagaaaaaac aaatgaaaaa gtagttctta
      721 ttgccagtgg agatctctcc caccgtttaa aagcagacgg accttatggc tacaatccct
      781 ctggtgaaaa atttgatgat gccttcctta ggctcttaga aaatggccaa gtcacagaaa
      841 tctttaccat gaacaaagaa atcatttata atgctggaga atgcggcaga aaatccgttc
      901 ttataatgct tggtgccatg gatgatatcc attttaaagg tcatttacta agctatgaaa
      961 aaacctttgg agttggctat ggcattatga aatttgaaag tacaggaccg tgccagtcca
     1021 aattaaacac cattgttgat gctgtaaaaa ataaaaacaa caatcaaagt aatccctacg
     1081 tccgtttagc cagggaaagt cttacaactt atctggaaaa gagaaaaatg atggaagaac
     1141 taccttccta tgtaacagat gaaatgaaaa atttaaaaag aggcgttttt gtatccttaa
     1201 agaaaaatgg aaacctacgg ggttgtatcg gaacgatttt ccctgcaacc agttgcattg
     1261 ctgatgaaat tatccgaaat gccattgaag caggacttta tgatcccaga tttgaagccg
     1321 tggaattatc tgaattaaac gatatagaat tttcagtaga tgtcttaaca gaaccagaaa
     1381 cggctactaa agaagatctt gatccaaaag aatatggcgt tatcgtcaga agtaaaggaa
     1441 gaaccggact tttactgcca gatttagaag gagtagatac cgtagaagaa cagcttagta
     1501 ttgctcttcg aaaagccgga atcagccctt ttgaaaacta ttctatagag cgttttaaag
     1561 tcatccgtca taaagaagaa taaggaggct gcataagtga aagttgaagc ctgtttttat
     1621 gaaaaacata gagataaaat tcactgcttt ttatgccctc accattgcct tattgaaaat
     1681 catgaatggg gcaaatgcaa ggtcagaatt catgaagacg gagtccttta tagcattaat
     1741 tatggagaaa taacggctgc tgcccttgat cctattgaaa aaaagccttt gcattattat
     1801 aagccaggaa cttttatact ttcagtgggg agctttggct gtaatctcac ctgttctttt
     1861 tgtcaaaatt atagcatttc ccaatacaag gcccaaagca cttatatagc aaaagaaaag
     1921 ttggccctta ccagcattaa catggaaaat aatattggct tagcttttac ttataatgaa
     1981 ccaaccatct ggtatgaata cgtttacgac acagcaaagt atttaaaaga aaactttcct
     2041 ttttacgtca ttgtcttaat tacaaacggt tatatagaag aagaagcttt aaaaaagctt
     2101 cttccttatg tagatgctat gaatattgat ctaaaaagtt ttaatgagca atattataaa
     2161 gaactttgtg gtggcaggct taagcccgtc ctaaaaagca tagaattagc cgcaaaatcc
     2221 tgtcatctgg aaatcacaac ccttttggtc agcaaagaaa acgacaatct tacagaaatc
     2281 gaaaatatcg caaaatttat aagttctatt gatagcaata ttcctttgca ccttacccgt
     2341 tatttcccta attataaatt aaatcatccg gctacagata aagaaatcct gtataagagt
     2401 ttagaaatag ccagaaaata tttatcatat gtcaacttag gcaatattta agagaccttt
     2461 tctatcctgt cttttaataa aaaggcaatc atactgcctc caatgcctcc ggaaatgact
     2521 tgaagaatat taaatggaac agcactaaga gctgatgaaa gagcagcttt aaaaccttca
     2581 gaagaaaata ccagtataac gcctccaata taataagtaa ataccatcac aaaacctgaa
     2641 agacatattc ccagaaaata ttgcttttta ctttttgttc ggtagcacat tttaccagca
     2701 atatatcctt ctaagccttt tgccagcaaa gttataggta tccaggctgc ataaccactc
     2761 aataaatctg ccagtgctga ccccagtcct ccggcaataa aaccaacttc cggaccaaaa
     2821 ataagagcag atgaaaaaat tagaatatct ccaaaattca gataaccttg cagtcctatg
     2881 ggtatagcta tgaccatcgt cactatggtt accaaggcag ccattaaggc cgtaatggtt
     2941 aatttttttg ttgtcatctt gtctccccca ctccataata taaattcata tttttatata
     3001 ttttaacggt ttatccatta ttctgcaaga aataattaag aaatcattga tcatcagaaa
     3061 cgggagagag gcagagcaat acccggttat tttattttat ccacatcttc tttattcttt
     3121 atattttcat tatttttgtc aatacgacga ataaggttaa tttttctttt aatattgatt
     3181 atatctaaat aatccattaa tgacttatta cctgttcctc tgtttttttc tgactctctg
     3241 ttatttttgt ctatactcca tctaatgata gcctccaaat attcaggctg cctggaatca
     3301 tctttatctg cagcatagac taatgcatct cttagcattt gcgcattttt actaaaattc
     3361 tctcttgaaa tataaactcc ttgttcttcc atatattgaa taataaaagt tgatattgtt
     3421 ctcgtatttc cttccctaaa aggatgtatt ctccataact cagcaaaata cttggaaatc
     3481 tctgaaatct tctggtcaaa agtaagatta ttccaaggaa ctgctttcat actattaagt
     3541 atcatttctg acgatttttc aatatttttg catgtctcat actggattga acggtgatta
     3601 agtataagct catgccgtat aatatcttct tcccttagat gtcctgccca gtcataaata
     3661 gggctaaata aaaaccaatg tatttcacgc atatgtgaaa aatcaaactt acctttaatt
     3721 gggtatttag ataaagtatg taaccggtca gaaacaattt tattttctac ttcttttaat
     3781 ttttttgcat ctttaatatc aaatttattc ctgagaacaa aaacatcatc atatagatat
     3841 ggctcataaa tcataatctt tcactcttct ctatttattg ttgaaaagat taaaatactc
     3901 caatacctat tataacgata attcttattt agaactttga gtgtctctaa aatatcctgc
     3961 ttttattatg tctctattca taccttaagg cctcaatagg atccagttta gctgccttat
     4021 tggcaggata aacacctgct ataataccta ccaggcttga aacaccaaca gtaaccagaa
     4081 ttgaaaatag agaaaccttc ggggttacat ccatcagcat accaaccaaa gaaccaccta
     4141 aaaatcctgc aataatgcct attattcctc caatgacagt taaaataacg gcttctatca
     4201 ggaattgctt cctgatatct ttattctttg cacccagaga ttttcgaatc ccaatttctc
     4261 ttgtcctttc tgtaaccgtc accagcataa tattcataac accaattccg ccaacaaata
     4321 aagaaatccc cgcaacaaaa ctaataaatg cagtaatata gcctaaaact tgattgatca
     4381 tttctatctc atccattaaa cttctaatat aataggcgtc ttcattccta tgcttcattt
     4441 caagcatctt atttaattcg atggccgttt ggtctatttt ttctggatct ttaacactaa
     4501 cataaatata gtctaaataa tctactaaat aaatatccat aactgtttga ataggcatat
     4561 aaagagcaga tggcatttct gcccccataa tactttctaa agcagcattg gaattctttg
     4621 tgactccaat gaccgtaaat tttaagtttc cccgccataa tctaacggtt atcgtttcac
     4681 ctacaacatc tttccggcca aaaacctttt cagccaggac ttcatcaata accacgactt
     4741 tactttttgc tttacagtcg gactcgataa taaaacgtcc gtatagtaag tctggtggaa
     4801 ccataaacct gtattcctca ttaattccta taatataacc gctcttttct tccttgggat
     4861 tttttaattt tatcgatgca ccccaggcct caaaatatgg agtcacattt tttacttcag
     4921 gatgtttttt gattaattct acatcatcca aggtaagaaa atctcttaat tttgcatcat
     4981 tgcttccttt catctgaatt gttaacaagc caacacctaa tttatcgaat tcagatgtaa
     5041 tggctgctct gcttcctgcc ccgatagaag taatcataat aacagaagca atcccaataa
     5101 taataccaag catggtcaga aaggaacgca ttttattgga cattaaattt ctcagagctg
     5161 attttaaact ttcaaaaata ttcataatgc atccccccca acccggatgg gattatctac
     5221 aaacttatct gttatgattt ctccatcctt aaaagtaacg atgcgttttg tatgatgagc
     5281 aatttcaggt tcatgagtga ccatgactat tgtaacaccc tcatcgttta acttttgaaa
     5341 aatacccata atctcctcac tcgatttgct gtctaaattc ccggtaggtt cgtcggccag
     5401 taaaatggac ggagaattca ctaatgccct ggcgatggca accctctgtt tttgtccgcc
     5461 agataattca ttaggcaaat gaagcaccct gtcggacaaa ccaaccttat ccagggcagc
     5521 caaagctttt tctctgcgtt ctttatgctt agcccctgca taaatcattg gtaactctac
     5581 attttctaat gccgtcagtt tgggaagaag attgaacgcc tggaatacga atccaatctt
     5641 aagatttctg atttttgcta attcatcatc atttaaagaa gacacatcca cttgttctaa
     5701 gaaatattta ccgctatttg cccgatctaa acaacccaaa atattcatca gggtagattt
     5761 tccagagcct gaagcaccca taatagcaac gaactctccc tgagatacgg ttaaattaat
     5821 atttttaagg gctgcaacct gaattctgcc attttcataa attttgttaa gagattcaac
     5881 ctttatcatc tcattcacct gattctacct tattttctat aggttttact tttattcctt
     5941 cctgaacctg ggcagtaata ttggtaatca ccttttctcc ttctttaacc cctttaactt
     6001 ctacatacaa atccgaatat gctcctactt cgattttacg tttttccacg gtatagtcct
     6061 ctttgacgat atagacaaag ttctcatcat tttcttcaaa aacagctaat aaaggaatga
     6121 ccactgcatc gttaatttct gcagtaataa tatctgcatc cacactatat ccaggtttaa
     6181 gattagctga attctttgtc acttcaattt caacctcaac catgacttcc tgagaacttc
     6241 ctaaattgat gttcgtagca acaggtgaca ctcggataac ctttccttca attttttcct
     6301 ttttaatagc atctcctgaa aaagtaacct tttgtcctgg tttaatagaa ggagcatcat
     6361 attcagatac ctgaacacgg actatcagct tatccagatt tgcaattttt aatacaggag
     6421 aattttgtgc aacccatgtc cccgattctg caaaaacctc aacaactgtt ccatccatag
     6481 gactttccat agaagccaga agttcatccc ttttaagcct taaagcttcc actctcattc
     6541 tggctgcttc tacctggttc tgttgaattt ttatttcatt ttctttctta gaatctgcat
     6601 acttgttata tgtagtggaa agattgtatt ctgctaaagc tttttgcttt cttgcatttt
     6661 ctatatttaa cttttcagat gcgattctca tttcttctgc ttctaactga cttcttaatt
     6721 catttaactg attaatggat tgatccaatt ctaatttaga aattgccccc tgattaaaca
     6781 aaattttatt attagccacc atttgttcag catctttaac cttttgcctg agctgttcaa
     6841 tgttactttc cgcttgcttt aaagatgttt cagcatcatt aagggctttt ttagccatgg
     6901 tataggctga ttcggcattt aaaatttcac ccttatcagc ccctgttaaa gcgcttaaag
     6961 tcaattctgc attttgtaag gctatttctg cttcttttaa ttggatttca atttcagcta
     7021 aagaattgga atcgtattca ataatctttt gtcctttttt taccatatcg cctggttcaa
     7081 ctaaaatatc atcaattttt atggaactat ttacatatac aatttccgag tccttttctt
     7141 caacgactcc ggaaacatta accgttgata taatggaatc ttttctggct tcttcaacag
     7201 caactgaaat cgcgccttta ggagctccgg aagcagttac tgatgaagat gtatttattg
     7261 cagcagcaat aataaatatc aataaacaag ctgctatacc tattccaata aatagttttt
     7321 tattttttac actcatattc ttcatggata ctctccccta ttctattgat tacataaagc
     7381 tactcaagtt acttaaaagc ttaaaagaaa aatagccata aagggcagtt aacaatttag
     7441 ctgaaaagaa atacaaaaaa gcaatgatga accctttttt cttagaaaaa ttattaaaca
     7501 aactaattcc catagctaca ataatcatcg tccatattgt gaaaaattcc agattagaaa
     7561 agaataaata gataaaactt gttatatctg gattcggaat aaaggcaaca ggagaaaata
     7621 taacaatatc cgtttgtaaa atcagattaa acggtgccgc aatcagtgat ccagtcacta
     7681 aaataatcaa agcatgggca tataaagcaa gcatttgttt aaaagagctt ttacctttaa
     7741 agactttaac caatacccac aatacaaaac ttacaaataa ccacgaaaga attaagaaaa
     7801 caggagataa tacagctgaa atgacagatt gtgcagtact tatttcagtt tgtacaggca
     7861 taccatattg tgcatagata tcattcagtt tttgaaggtt aatcctttgc acaggaattg
     7921 taattaattg cttaagaaac atgagaattg aagaatacaa aagaggcatt aataatccag
     7981 gatgttccat gacatcattg agtacttcag aaggacttat aaatatctga agtatttttt
     8041 taaaaatgcc catcggttta ctggcagtga cttcaacttc ttgcttgatt gcaatatttt
     8101 ccataaaata ctcctttcat gctttatatt aattgcgaaa atcaaaattt tcaatatacc
     8161 tttcatatta tacgttaaat ttttaaagta tgttaaaaaa taagagggtg aaaaaaacac
     8221 ccaaaatgat taaataaaca ctttaaatac attattattt taaatagtca ggattcaaaa
     8281 cttttaattc aggaagtaca aacaaaccat cttttctaat gagaatatcg tcaaaatata
     8341 tttctccgcc accgtattca ggtctttgaa taagaactaa atcccaatga acggcagatc
     8401 tgtttccgtt aaagcaatca tcataagcat taccaggtgt aaagtgaata gagcctgcta
     8461 ttttttcatc aaaaagggtt tctttcatag gtttcgtaat ataaggatta agcccgatgg
     8521 caaattcacc aatatatctg gctccttcat cagtatccag aattttattt attctttccg
     8581 tatcattggc tgtagcacga ataatttttc catttttaaa ttcaaaggat atattctcaa
     8641 aagtaaaacc atgataaatt gaaggtgtat tatagctaat tatcccatta acagactccc
     8701 gcactggggc tgtataaact tcaccatctg gaatattcat ttttccatca catttaatag
     8761 ctggtaaacc cttaattgaa aaagttaaat ctgttccctt tccaacaatc ctgactttgt
     8821 ccattttttc cattaaatcc accagtacat ccatagcctt tgacatttta gcataatcca
     8881 aattgcatac attaaaataa aaatcctcaa aagcttctgt actcatatca gccatttgag
     8941 ccatagcagg agaaggataa cgcagcacaa cccattttgt ttttggaacc cggatgtcca
     9001 catgcactgt tttccaaaaa tacttataat acaaatccat tttttcagaa ggaacatccg
     9061 acaattcaga agcattccgg atagaagtaa aaccaataaa agcatccatg tctttcattc
     9121 tgtcagcttc atatttagcc atggcttcca tatgttcttt tgtcgtattc atatataatt
     9181 ctcttaaaat cctttgatca ttcagttgaa caaaaggata tcccccaact tcatatactt
     9241 cttttataat ggcattaacc agtgaaactt ctaaaccgtt tccctggatt aaaacttttt
     9301 cccctttttg aagatttaca gaatatctta ctaaagttct ggccagcttt ttaattctgt
     9361 catcgtacat tttatcatcc tttcaaagta tattcatttt ataacagtta tatatcagta
     9421 tctttgtact atatgttatt ataccatgcc ttaagaaaaa ataattttat aacatagaag
     9481 ttttacaaaa tttattatgg atatagaatt ttgatactag cttttcagta caatactgta
     9541 tagttttctg cattgacaaa ataataaatg ttaaagaaaa atttaagatt tattcgagat
     9601 ttgcaaaggt atccgacgaa gtcagaaaac catttagttt ttctttattc tttaaaaagg
     9661 tgtcaggttt tataaaaatt ttgtgtatga tattatgaat tcctgaaaat ataaatggct
     9721 tagagaaata taatccctaa gccaataatt aaataattac atttttgtgc atagcataaa
     9781 gaatctaaaa cagagatttt tgtattttat tctgtttttt cttcaacgtt ttcttcagct
     9841 tcttcaactg tttcttctgt ttcttcagct tcttcaactg tttcttctgt ttcttctgtt
     9901 tcttcagttt cagcttcttc agctacttct tgctcttcct gaacttcttc gtcaacaatt
     9961 tcttctgcat tttcttctgt gctttcttca gtactgcttt cttcagcttc tgtattaatt
    10021 gttatggtct ttgtttcggg atcccaatca gttacagctc caaaagattc agaaacaaat
    10081 tttaatggaa ctaatgtcct gccatcaatt gcgataggtg ctgcaagtaa ttctacttct
    10141 ttatctccta cttttgctat cttattattt aattgtaacc aaataacaat accatctttt
    10201 tcagcagtaa tggtttggct ttcaccgtcc cattgaacag aagcgccaaa agcttcaaaa
    10261 acatgtctta aaggtattaa agttgtttga ttttgtacaa caggggctac atccagctta
    10321 aattaaaaac atcttttatt ttcttttctt aaacatactt ctcctctgcc acagcgatag
    10381 caaacttcat tttccagtac tttatcatca aaataatctc tgcaattttt taaagtcgtt
    10441 tcggcaatat tatgtaaagc ttctttggtt aaaaaggcct gatgggaagt tacgataaca
    10501 tttggcatgg ataaaattct tgccaaaaca tcatcttgga taaccacata ggacatatct
    10561 tcataaaaaa gctcagattc ttcttcataa acatccagac aggctccacc aattttctca
    10621 ttttttagcc cttccagtaa agcttcacta tcaatcaagg ctccccttga tgtattgact
    10681 atatagacac cttttttcat cattttaatc gtttcttcat taattaaatg gaaagtatct
    10741 ttagttaatg gacaatgaag agaaatgata tcggattctt taaacagttc ttctaaggag
    10801 acataatgaa tatcagaatc ttttaaagga tatgggtcat atgcaataac tttcatcccg
    10861 aagcctttac aaatatccat aaatactcgt cctattttac ccgttccaat aacacctact
    10921 gttttcccgt gcaaatcaaa accagtcagg ccatttaagc taaaattaaa atccctggtc
    10981 cttatataag ctttatgaat ttttctattt aaagccagta acaatgccat cgcatgttct
    11041 gcaactgcat aaggtgaata tgccgggacc cggacaacat gaatcttacc ataagctgct
    11101 ttaaaatcaa cattattata acctgcacat cgcatggcaa ttagtcttgt accattttga
    11161 taaagggttt ctatggtttc tttgttaatt gtatcattta caaaagctac aaccgcttca
    11221 tagccattag ccataaaggc tgttctagga ccaagtttgg gctcaaaata atcaatttct
    11281 ataccatacg cttcctttaa taaatcaaaa tacatcttgt cataaggttt tgtgtcaaaa
    11341 aagcatatct tattcatcat atcccctcct aaaatcataa agcactttac tttatttttt
    11401 aaattaatta taattgacaa ttctgatttc aaaaagtcta tcccatggta attcaacttt
    11461 tttaatggca attttatccc aatatacatc atcaaattcc attaagaagt cactttgaag
    11521 ctttatgaaa tgatccgtaa tatagctttc aagataactc tttccataag aagcatctaa
    11581 attatgaaca tcagaaccgg tagaaattaa ataactttct aaaacaggtt taattttaac
    11641 tttataaaca taatcattca taaaacgatt atataaaaac ttttgatgat attgctctgt
    11701 tcccagcaaa aatcgttttg aatgccaata agccaataaa tgtgaaatag atatcccgga
    11761 agcattggaa gccgtattcc atgcactata agagcccaag tctgataaat taatattttt
    11821 tcttaacagg ctgacaaaat cattagcaga actcttagaa agattattac ttaaatcaat
    11881 aatccctact gtttttccac tttctatata ttctttaatt tttttaataa agtccagagt
    11941 aatggtttca ttttctatgg aattataaac gaacaaatac aaatccgcat tatttgttgc
    12001 ataagaaaca taagaagcac cagcaagttc aatatgctcg ataatcgtct tatctaatgg
    12061 tctgtcttcg taagaaagaa tgattccctt tttattttcg ttgatgtatt gaactgatac
    12121 tttgggacta taattataat gttttaagat gtgcctcatt agtaaattca attcaacttc
    12181 gtctgttcct ataatcattg aagttttatc ttcaacaccc agttcatgga ttttattttt
    12241 tatttgctgt tgatctaata agtgaatacc cttttcggct gcatcatctt gtgataaaac
    12301 caaataatca atgacgcctt cttgaaccag attaatggct tcaaggttaa aacttaaatt
    12361 aacagcacgg ctataaagat atttgttttt cagttcaccc ggaatttttt cttctaacgc
    12421 ttttaattta ttcctgtccg ttatcatata gttttttgcc caattttgaa tattatcata
    12481 aagctctttt tcgtatttat tgtctacaga aaccgaaagt ctttgaatcg tatcaaaggc
    12541 ataaatttct ttaccgggat ttttgtcgtt taattctctg agccaatcta aatcaatcac
    12601 aaaattatca ttatctaaca tacgggaatg aattaagcct ccgtggttgg tcatggtaag
    12661 agatataata aaggcatctg catatttaga attttctaat gcccactgtt ttattgattc
    12721 aatatctgct tttttatgac gtccgcctaa aatctcattg ggaggcagta aaatatctac
    12781 ttttgcaata tctgctatag ctttaggaaa atcagtatta actggcctgt catctaatgg
    12841 gactatcatt attgttaatt catttttttc atctgcttca ttgtaattcg taaaacctgc
    12901 cataaaaatg gatataaaaa aaaccaaaat aaaaaaaaga tatcgtctgc tcatggatta
    12961 atccttcttt ctcataagta gttttaatgc gggctaagaa gtaaaaagat taaattcaaa
    13021 aaatcattct ttatcctgct tttttacata aatccatata taaaaaatca attacctgca
    13081 atgtatcctc ctgataaaac ttatttattt ttgtaattta aatttttgtt ttcttatcag
    13141 tgcttcgatg tctttgttta tgttgcagga aactctttaa atattagatt cattatgttt
    13201 tgcaataaag aaatactttt atcattactt ttatcatgtt caaggaaaaa tatatttcct
    13261 ctccaactat taaaactctg taaaattcag taagtgcttt tttaatattt ttaatacttc
    13321 aaagcaaaag tgcttgacgg tactgtcaaa gaatgctttc agaaataatt attaaagtgt
    13381 caatatttat tttattattg ataaatatag tatgatatta aatagtgatt tctattctgt
    13441 taccatccgg atcctcaata acgctttcaa aatatccatc accagttgtc ctggcttcac
    13501 ttaatacctt ataaccatcc cggcgtagtt tttctgtcaa gtcaatgact ttttctttgc
    13561 tgcctacaga tatggctaaa tgagctaaac caatataatt tttttcatca ttacaatcca
    13621 tttttataag ctttggtcgt ttcatgattt ctattcggca ttctccatct ggaaaagaca
    13681 aaaaataaga ttcaaagttt ttggatgcat taatatactt ttgatttgcc tgcgcatcaa
    13741 aataagtgac ataaaattct ttaagtcttt ctaaattatt tgtccatatg gctatatgtt
    13801 caactctcaa aataattcct cctttattga catgatttct tattaataat actttaaaaa
    13861 cggtcctgat tcaataataa ttgaaaagga ccgttttttg atatttagta tcagcagctg
    13921 taaatgctct gatttcaaaa atcattgcta ttcaggatat tataatattg tcggaaactt
    13981 tccggacttt ctgcaatttc agagctttta caagacataa gtgacatact taatgcatct
    14041 gcatcttttc tgtcggtata aaaaataatt tctatcgaat taccatcatc tatggcatat
    14101 tgaatataat ctaaattatg aatcagggaa aataatatca gagactgagt tctccagata
    14161 taatcagaat tcatggacat atatttcttt tttgccccct catcagccat aaaatttatt
    14221 tgcaggccat agggctcttt ttttgtaaac agttccattc cattagaact caaaccttcc
    14281 ggaaaattta aataacgaat aatctttccc accctgacat tatccccgat atatgttcct
    14341 cttagctcat ataattcatc cactttgtct ggctcagaat tttctttctt caaattctta
    14401 ttaaattgat ctcggttact gatataaata tccagggaca tttctgtgct gatatgtcca
    14461 taataaccga aactttcttt tgcatcagca aacacgatat tatctgcaaa aatttcttcc
    14521 tgaccatcag ataatttaat caaactgaaa tatccattgt ctttatagcg gataaataat
    14581 atctgctgat tggataaatc cattggtaaa aagtcaaaat gattaccttc tgtaagtctt
    14641 tgtattatag aacttttcaa atcgtattcg taaatattaa aagctggtat tttattatta
    14701 aatataatat cattaaaatc ttttgtatca gctgctgaat acaatatttt ctttccatcc
    14761 aaagtaaatg aaggggtcat tgcaacaaag tcttcatccg taatgtctac agtgccacca
    14821 gatatattga atatttttac ttttttattt ctgctcattt cccggccggc accttctatt
    14881 aatccaacca tattggcaaa aggtcttacc gctaaatgat ttttataagc taaagtttct
    14941 atttccggat actctttata agttttttct tctgaatcat atactgcaaa agtaattcca
    15001 tcactactaa gagaagcaga agcaggtata accatgatat aaagatatct gccgtcctgg
    15061 gaatgattcc agacaacagg atagctgtcc agcccatctt tagtggattc tttacttttt
    15121 ataatcgtat ccgtaaaaat tgttgcttat ctttactata ttgatttaaa tcaatttcca
    15181 caattccttc ataaatcatt aaaccatttt ccgatcggct tttacttgca taaactttct
    15241 tctgatccga tgcaactaaa gctgtataat aagcatcctc taaatgaggt gttttttctt
    15301 ttgtaatgac attataaaga ataaatccat atctttcttg actgccatat acaatagaac
    15361 gatcatctat ccaatcataa gaagtaaaat gatgaacgat atcatcatca accttttcta
    15421 aactgctatc tcttaagcta tagatataaa ggctattatt tttggtataa gcgatataat
    15481 ttcctccttt tgaaatcaga ggacaaaaaa agtcatcacc atcatctatc tttatttctg
    15541 cctgcatatc taaataagaa taataaagcc catcttcttt agtatacacc acaaaagatt
    15601 tttcattttt ccattggtta tcagcgaata gactataggc aaccgtaaaa ccgcatgtca
    15661 caatgataat gcctgaaatg aacgacataa attgtctttt tcttgttgtt ttcagggaat
    15721 aaaacatatt ttccaatctg tcttttaatt gcctgccacc attactcatc gctgttgtaa
    15781 gagaacaatt tctaaaagca gagttgctga ttaaatttat aatcatttca ccataaaatt
    15841 ttctatcatt tttgtccata ttttctacga ctttctcatc aacagaatac tcacagtatt
    15901 tgttcatctc ctttaacagg agataaatca aaggattaaa ccaatgcatt gcatgagcaa
    15961 aaagcccaac tgtttttatg ataatatctt tccttttata gtgatttaat tcatgtaaaa
    16021 agaccaattc taaaccttta tggcctaaat ctgtttcagg aataagaacg attgggtgta
    16081 aaatccctat tagcattgga gaagcaaggt tcttataaat tcttaattct aattttttat
    16141 taatatgaag ctcttcttta catttatcaa atagctgaat cacttcatca tttttaacaa
    16201 aactgctgct tttaagcatg gttcttttaa atgctaaata agatatgatt tttaacaaga
    16261 ataaagcaat aacgccaata agccaaaaat acaataatag atttttatat ggataaagat
    16321 taattttttt gtttaaaatt gtattatctt ccagtccgaa ttcattgctt tctctttctt
    16381 tatagttcgc ttctttttct gaaatatgca cttcgtttgc agtactttca ttatttattg
    16441 ggtttgtttc gttttttctt tctgctttat tatttttact ttggggaaaa attgattctt
    16501 cgattctaat ctttggaatc ctggcaaatt gtcttacagg caactgaaga taagaatcta
    16561 taggtaaaat aaaaaagcat aaaaagacaa taagcatttt ataatgccag gaagaattaa
    16621 ataatttctt cgtcaggggt ttaaaaatta aatataacaa aaaaataaca cttcctgtca
    16681 gagacatctg aaagattttg tttaaaaagt ccatattata acccccttat ccagtctttt
    16741 aattcttcta aatctttatt atcaattttt ttattatcat ataaggaggc taccatagat
    16801 ttaatacttc cgttaaacat ctcctgaata agtccacttg cctggtaaga tttatattct
    16861 tctttggtaa ttttaggaga ataataattg actctctttt cctttcttac ttgcaaaaca
    16921 ttcttatcaa ccaaacgctt ggcaagggta agtacggttg taagtttcca gcttttattc
    16981 ttctccaact tttccataat atctgccgta gttacttccc tttttgcatt ccatatgatt
    17041 tccattattt cgtactctgc atctgatatt tttataatat tcataatact cacctcttta
    17101 ttctacatca tgtagtattg attttattct acttattgta gaataaaatg tcaagaggat
    17161 gaggataatt tcatggatta tatttttctt aatagctttc catatgggta tgaagtgtct
    17221 tttattttca ccagcttaag tttattttct tcaatcattt tatcaatacg taaagcatac
    17281 caggcatcgc tgataccaag cttatatttt cctaaaagtt tgccaatcaa ttgtcccatc
    17341 agaaaatcat tgtctggcaa attatttata ataataaaat cataaaaact ttcttcaact
    17401 gaagtcaact ttccattgat tacagcacgt aaaggtgcat tttctaatac taaatcctgc
    17461 cacttttgac tataatactg cttttctatt gaactcagag gcttttccag atttaagaac
    17521 tgatagaatt ggcctggctt gatttctgcc cagtgcgaac attctataat ctcatgatta
    17581 gaaaatgata taaattgagg aagagaaaca accctaatct tacagtcaat atttcttaat
    17641 agatagcaga cataataaaa tccacaaaca gaataaggcg catgacttgt ccaaatccgg
    17701 atatccatac cttctttagc agcagaaatt aatttctcca gatcttttct ttgatatttg
    17761 aaaaatagtt ctctttcatt atcttcaata ttaaaacgtg tccataactg gcttaaaact
    17821 ttttttcttt cactcccatc aatctcacct gatatatctc caatatcaag ataaaaacct
    17881 atattaacaa cattattagc atctccttct atggctttcc cttctaaaaa aagctctatt
    17941 tctgcttcag tcggttggct cccaatataa gtaacagatc tgccaagaat tttttttcat
    18001 tataacattt ggcaagtttc atagaagctt tttcgctatc actaaataca acttcaagca
    18061 taaaatacct cctaaaaatc aaatcttaaa aattcatcat aattttaata ttactataat
    18121 aaaacaaatt attcaaaaat ttattaagtt tggaataaaa tactcatgac ttttccaaaa
    18181 ttaaaaataa agcgatcaac tcaaaatgtt gaccgcttta tcttaaatac gataatcatc
    18241 ttctaaagct tccattaaac catattcaag taaaatttct tctaatcgtt cttgcgacat
    18301 aggcttaggg atggtaaaaa agtcgttttc atcaattgct tttgctaatt gtcgatattc
    18361 attagcctga tggcaattgg gcttatattc tattactgtt ttccggttaa tttcagcatg
    18421 ctgtaccaca ttgtcgcgag gtacaaagtg aataagctgt gttccaagtt ctgcacaaaa
    18481 ggctcttaat aggtcaatct ccctgtcgac attacggctg ttacagataa ttccgcccag
    18541 gcgtacccca cctttttggg catatttggc aatccctttt gcaatattgt tggctgcata
    18601 aagtgccatc atttctccgc tggcaacaat ataaatttcc tgagcttttc cttcacgaat
    18661 cggcattgca aaacctccgc aaaccacgtc tccaagaaca tcataaaata cgtagtcaag
    18721 gtcttctgta taggcaccta attgttccag caatccaatg gacgtaataa tgccgcgacc
    18781 agcacatcca acaccaggtt ccggtcctcc ggactcaaca cagcggattc ctccaaatcc
    18841 agtacgcatg atattttcta attctacatc ttcacctgtc tcccgtagtg tatccagaac
    18901 agttttttgt gcaagagttc ccaaaagtag tcttgtagaa tccgctttag ggtcacagcc
    18961 tactaccatc actttttttc cgctttctgc aagggcagct gttaaatttt gtgttgtagt
    19021 ggattttcct atgccacctt taccataaat agccacttgt ctgatttgtt tactcatagc
    19081 ctctatcctc ctagtcttta ttatattttt atatgatata ctctggttct ggtaaagcac
    19141 taaccagatg aattttttct aatatcgtcc ggcgaaggta tagaaaatct gttcctccac
    19201 gatggcgggg atggggcaga ttaatatcca taatctcact aatttttcct ggccttggtg
    19261 tcatgatgac aatacggtca gaaagataaa tagcttcatc gacatcgtga gttactaaaa
    19321 tcatggtggt ttcattttct ttatgcagct ccagaagttt atcctgaata tctgcacggc
    19381 taaaggaatc aagcgctccc attggctcat ccagaagcag cactttcggc ttattaatta
    19441 acgctcttgc aattgcgaca cgttgagcca taccgccact taactgatga ggataggctt
    19501 tttcaaaacc tctaagccca actaagtcaa tataatgctg gacaagctct ttattttctc
    19561 tataaatccc cctggcccta agacctgttg caatgttttt ctctacggtc agccactgaa
    19621 aaaggcagcc atgctgaaaa acataacctc tttgagggtc tggttcagta attttctctc
    19681 cattaagaat caaagttcct ttttccggtt tttcaagccc cgcaatcagc ctgagaagcg
    19741 ttgttttacc acaacctgaa gaaccgatga tagagataaa ttcgcctcgt acgacggaca
    19801 aattgatatc ccggagtgct tctacatgat taccataatc gtcataataa gtatgactaa
    19861 cattttcaat gcgtaaaatt gttcgactaa ttaccgataa cgtttcactc attcttttac
    19921 ccatcctttc tgccagcgca gcacatgggc ctgcactaaa gcaattcctt tcataatcag
    19981 gctaaaaagt attgccatca caaaaataga agcaaacact ttataatatg ccgaccagac
    20041 tttacttaaa ttgatataat agcctaaacc accaggctgg cccatcattt ctgccatcac
    20101 caaagtggtg aaagcaaaac catttgcatt ggcaatacca gtaaaaattt gcggcattgc
    20161 atgaggaaca gcaacatgaa agacgagaaa aaaggtactg gctcccaagg tacgggccac
    20221 ttccaattga actttgggag tcgacttaat accctgtgct gtcaaagccg caatagaaaa
    20281 ccagccgcag attacaatta aaaaaaccgc tgcccaaaat ggcgtgggga aaagggttaa
    20341 agcaaaaggc atccaggcga ctgcaggaat aacgcctgta agttttaaaa tcggataaag
    20401 ccaataataa gccttaggaa accagccaat aataattccc gtgccgatcc ctaagagcac
    20461 acctaaacta aaacccacgg taaaaagctt taaggaataa agcatattac taaagataaa
    20521 gtccccttcc ataagaaaag gctcaagaat ttttgccgga ccagggaaaa atggttgcgg
    20581 caacaaaaga tactttgtcc ctaatatatc ccatatggca agcgctaaac ccacggcaaa
    20641 acgaaaagga gcacggcttg aaaactttac tctggctgaa ggattaaagt atgaaaaaat
    20701 tccagcaatt gcataaaata ataagagcac gccaagaagc aaacggtaaa aggtattaaa
    20761 agtaaaagga attccaaaaa tcatataagt tttaatttca acatttgcca tttgccttat
    20821 gaacttattg gcagctatgg caatcaaaaa accgagcaag gttaaaataa agtaaaaaaa
    20881 ccactgtttg tcagacaggt tttttagctg tattttatcc ttttttgttt taaaaattgg
    20941 aatggtttga gcctgtaatt tgtttaactc aaccatgttg aaccctcctt tcctataagc
    21001 tttattgctt ccgcccggat ttatatttcc gggcggaata tcaataaaat ttgcctgttt
    21061 ttatttccca agattaatat ctacttcagc ataagcatta tttgtaaagc tttcaggatc
    21121 cgtttttaaa tagcctattt gattcaattg ctctgcaaaa taataaacat tgtcataaac
    21181 ccgtattggt tcttcttctc tatctgcata agaggggtat ttatagcttt taattaactc
    21241 aagagctaat tccctgtctt caatttgggc atatttccct tcgataatga tatcaacggt
    21301 ttcttccgga tttttggaaa tccagtcctg ggctgcccgg taagcgcgaa gcagtgctgc
    21361 gatcttttca ggttcttcat taagcagttt ttcagatgca taaaggaaac agcagtactt
    21421 tccagcaaat ggttcatctt tagtaatatc caaaatgaca cggtagtttc ccgttttttc
    21481 ctgaacggaa ccaaagggat cccacaaagc tgcaacgtct acttctccct tatttaaagc
    21541 ctctattgcc aaattgccat cggcgaaagg aaggaaggtc acttctccat cttcttgttt
    21601 tgctgaaatg ccatttctct ctaaccatac agaagcaacc tggtgaggtg tacctccaat
    21661 ttcatccacg ctaattcttt tccctttaag atcatgtacg gtttggatgg gtgaatcctt
    21721 cggaacaatc aacttaatac atccgtcatg gagaccatca actaccttta ccttcacacc
    21781 attttcaata gaaggaaaga attgaaagtc tccgtttaca atcgggatcg taccattatt
    21841 taaaccaatc ttacgggttt ccgagtctgc agaaatcaga ttaacatcaa atccttctgc
    21901 tttaaaaaag cctttttcat aagcaatata aatgggtgct gcacataggg ctccgtcttt
    21961 accaggaata tcaatttttc catatttgta ttcagaggaa gacgtttcca ctgtttgaac
    22021 atcatctgtc ttaagagttt ggctttctcc tcctgagctt tggctgcagg cagaaagtaa
    22081 gttggcaaaa ataacaatta aagtcatcaa agcaattcct ttttgaacag acttcttcat
    22141 tggataacac ccctttcaaa aattaaacat aggctttttc aaaagcctgt tccaaatcgg
    22201 caattaagtc ttttggatct tccagtcctg ccgaaatgcg aatcaggttt tccggaatat
    22261 cagcaacttc tttttcatga gcttctaatt cactatgggt ggtctggggt gaattaacaa
    22321 tcaaactacg ggcatcgcca atattcacat gatagtggaa aagccttact gaatttaaaa
    22381 aggcttctct ttgctcattc gttcccttaa aaccaaaagc cagtattcct cctgcacctt
    22441 ttgggaaatc tcttttatag aggctgtggt atggactgtc ttctaaagca ggatagcgca
    22501 cccattccac agacttatgg ctttttaaat attctgctaa gatttttgca ttgcttactt
    22561 gtttagcaat ccgttccgat aaggtttcca gtccaagtaa aacaagataa acatcaaaag
    22621 gcgataatgc tgcaccaaaa taattcagat aagtaaaacg gattctcgct gtaaaaggaa
    22681 attctgggaa aacctctaag aaactgcggt attgatcatt gatatcacgc aaggtatata
    22741 gtttttcatg aaattgagga aacttttctt ttttccagtc aaattttcca ctttccagta
    22801 ctaaccccgc tatcacattg ccatggcccg ttaatccttt tgtggcagaa taaaccacaa
    22861 tgtctgcccc atggtcaatg ggattataca aataaggggt tgccaccgta ttgtcgacga
    22921 taagtggaat tccatgttca tgggcaactt ttgcaatttc agcgatatct aacagtactg
    22981 cactggggtt actgatgctt tccacataaa tcgctttggt atcaggcttt atttcttcag
    23041 ctaacttttt aggtttttcg atattttttg cgatatcaaa gtggattcca aattttggat
    23101 aaaccttttt aaagctgtca gcacttccgc cataaagata aggagaggtt aaaatgcggc
    23161 caccgccttc agcaacatta agaatggtgt agctaatggc agccatacca gaagctaagg
    23221 cgattgcgcc acttgcgcca tcaagagctg ccacccgctg ttccaaaact gctaccgttg
    23281 gatttcctac gcgagtatat aaattaccta attcacgtaa accaaagaga gctttagcat
    23341 gttctacgtc gcgaaaatca aaagaagtgg tttgataaat gggtaccgat acggcatagt
    23401 tatgctcatt tgactgataa cctgccttaa gtcttaaggt atcaaatcca tactcacttt
    23461 tttgttgatt tgacatacaa acactcctcc taataattga atggtttggt attgatacat
    23521 atcagccata tcagacatgt cacagtcact acacatatca aaccacctcc tttaagttga
    23581 tctttaaaca gctttattgt atcggccttg ccgctgctgc tattatccgt gagaaaaatt
    23641 attttcaaat tgtatggggc gcagatacaa ttgctgtgat acatcttttc cgcccggaat
    23701 gcctgctgca tctgcctttt ccagccggtt aatacatctt gaccaaagtt gattttccag
    23761 ctccactaaa accaataatt ccaaagattt cccctttccg taccgtcaga tcaattcctt
    23821 ttagtacttc aagagggcca ttaggcgtta catatgtttt atataagcct gaaattttaa
    23881 tatattcatc tataggttcc atattccgcc ggacttcatg atgattagcc atcttccatc
    23941 tcctttcact ggatcattaa agaatccttt tcggtcttta acaataccag gtataaaata
    24001 attgttttcg aaaaataaag tgtacttatt taattcatat aattcatata gacattatat
    24061 gaattaaaaa agttgctgtc aatgatattt ttcataaact tccaattttg cgcaatttgc
    24121 tgaattattg aattaagagt attccttttt tgtgcatttt tatattgcat atatgtttag
    24181 tatgttttaa taaaataaaa aatacctcct tttcggaggt atcctgatct aactttaaat
    24241 atagtacatt ataacgttat tggatttatg cttttctgct tcttttacca aatcttcaat
    24301 cgtgattgaa cttaatattt gcctgatatt gttgtctaat tgttcaaaaa ccgacagccg
    24361 catagcctca tcaatatcag gagctttttc cagaacagtt tcctgtactt gttcaaataa
    24421 agaagtttca acagcttcca atacatctaa agcagttatc ttagccgggc tccgggttag
    24481 ctggtagcca ccctgggctc ctttttcaga actaacaatt tccgccttct tcaataaagc
    24541 aaatacttgt tctaaataaa ttttagatat tcctaatttt tcagaaatac taatcactgt
    24601 aatattttcc cctgtattat actgctgggc catgctgata accgccgcca tggcatatcg
    24661 cccttttgca gatattctca ttgctctcac tcatttcata tataattact atgtatttat
    24721 gataataaaa aatctgcttc tcgtcaagca cggattgcta aaattcaaat agtctgtacg
    24781 attaataaat gattgtttct tatagctttc ttaataggtt cattttacaa attatatttt
    24841 attccttgta ggtagactta aaacgcattt ttggtgtgcg ggtgcttata tatacaataa
    24901 attttaattc cttataggta ggctaaaaac ttcgatttac ttagcactag atttacttct
    24961 tttacaggga aaatatcttc cgtcgcatta agaggaataa gattatagtg ataattttgt
    25021 tttttatact cttttccatt atttatattt tcaaagatag aataaagcct ttcattgatg
    25081 tactgacctt tttttatact ttctctttca tttccgtcca tttttcttct gtccatacca
    25141 gccgaaaaac aatctgcttc ttgtattagt ttttctaatt gattgtctgg cacatgatgt
    25201 tttgcagcca tattgattat attttcatca agcaccatct ttagattttc ctctaaaaat
    25261 tgaacagtat aaagagcatg tttgtaagaa aagtaaccac ccatattttt ataaaataca
    25321 ctttcttggc cttttaaact atcatttata ggcacgccag tccgctgcac caacttccct
    25381 atatcgtgta aaagtgcagc aagcgctaca tcatagttat ccataggcta cacctccttt
    25441 tcatagggta aatattttct gaaatttaca tgtttaaata ttcttctgga ctcaacttgt
    25501 ccatcgatga tatgttccac tatttctctt tgagttattg aatatattgg tattctgcta
    25561 tttttaatac aataatcaac cacataataa gttgcaccaa aatcgccttg aacaagtaca
    25621 taatcaccct tatcggattc tttttcaatc caggcaatca tttgattaag ttgatctttt
    25681 ggcataggtc caaatggact aatattggcc catatgcttt tgatgtcttc tggcattaaa
    25741 acaattgaat caacttttaa ttgatttttt gcttctaata cttgtccctc catcagagta
    25801 tgagaaatta aagctaaaag tttcatattc ttcactccaa aatttttatt atattgctct
    25861 aatcaattga atacatccca gcccctgaga gtttttgctg ccaagaccgc agtttaaagc
    25921 agcatggatt agctcctcat ccccatgaat ttcaaacaag ccactgcttc ccttgattac
    25981 aaactgttta tagtaaagaa tgttcatttg agtcttatcc tgtatggctt ttatctcgaa
    26041 ttttgcatcg tcaaatcctc taccataaat tagttcagct ttttttatca aattatcccg
    26101 tatatatttt tgaaaaaggc tatcttctgg aaagtaataa atcgtcttct ttctgccatt
    26161 taaatatacg gtagaatata ctgtaattgg cgacagagtt ttaaccaggg catcctttat
    26221 tctatagcta ttatcattta ctgctattct ctgaacttct agaagctgat tatttaacgt
    26281 taagttttta cgactaagac aggtcttcaa aaactcctga ttaaagtcat ctacaacact
    26341 ggatacagtt aatttaacac tttcggggaa gataattatt ttattttcct tatcgatttg
    26401 gtaatttcca taaagctgtg aaaaggtaaa taatttaaat tttcttcctt taaattcaaa
    26461 gcctttttca tgcaaaaata ctctaaaaat cattaaacct ctaatatgga aaacaattta
    26521 gctgcaattc taggaaattt gaatatatta ttccaccaat caaaaaacca gagtgaaaaa
    26581 taactctggt ttttatatac tatcatcttg tcaataaatt tcttcgttat aaaattttct
    26641 tatcaaatcg tctaaatttt tggctacccg ttcctgatta tctgattcat agtcccattc
    26701 aaaaacctca tcatctttta caagattttc tttagtaata agatatccaa aataaacctc
    26761 cgaaccatta ccggcaaaaa ataaatagtt gtttttatat ttaacacaac aaaattttca
    26821 gactattaaa taacactaaa tttctattaa aacagaactg ccctctgaat aatctccatt
    26881 ggtccactgc catttttcga caagccggat tttcccattc tccatgacag taggtacact
    26941 ttgacatttt cctgttctga tttctttgtt tttattgata tgctgatagg taaaattaag
    27001 tctgccatct tttgctactg tcccaattaa tgttcctttg atgatatctc caccagcata
    27061 ttctccccaa agcaaatctc cattttggtg ataataaaat cttgtctctt gattgacttc
    27121 accattttca gaattgattt caggaataaa a
//
LOCUS       DAOSUD010000023        26604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1202_length_26604_cov_21.707974, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000023 DAOSUD010000000
VERSION     DAOSUD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 26604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26604
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_26604_cov_21.707974"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1791)
                     /locus_tag="PLG49_04630"
     CDS             complement(157..1791)
                     /locus_tag="PLG49_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOQ16639.1"
                     /translation="MPITEFLERNAKLYGDEICLTEINLDLQEKHNVIWKEFELIENS
                     PLGEYRRDMTWRVFDEKANRFANLLIKRGIKKGDKVAILLMNCLEWLPIYFGVLKSGA
                     IAVPFNFRYSAEEIKYCLDLSDAIAVIFGPEFIGRIEAIYDQIPNVKMLFYAGENRPT
                     FAESYDRLTANCCSQPPNIPISDEDDAAIYFSSGTTGFPKAILHTHGSLVSACYTERA
                     HHHQTREDNFLCIPPLYHTGAKMHWFGSLLAGSKAVLLRGVKPEWILKTISEEQITIV
                     WLLVPWAQDLLDAIESGAVKLEDYKLDQWRLMHIGAQPVPPSLIHRWKKYFPNHDYDT
                     NYGLSESVGPGCVHLGIENIHKVGAIGKPGYQWEAKIVDDKGNPLQAGEVGELIVKGP
                     GVMKCYYKNPKATEEVLKGGWLYTGDMARMDEDGFIYLVDRKKDVIISGGENIYPVQI
                     EDFLRTHDSIKDAAVIGLPDNRLGEIAAAIIELKDGHICTEEDIIQFCKALPRYKRPR
                     KIIFDKVPRNPTGKIEKPKLREKYGAVRLVEAQTES"
     gene            complement(1972..2181)
                     /locus_tag="PLG49_04635"
     CDS             complement(1972..2181)
                     /locus_tag="PLG49_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HOQ16640.1"
                     /translation="MVLVKEFLGYEELQEKTVNRWLKEKGDSIEIIDIKYSVSCIQSS
                     LETMSPPREFSGILIIYKTKNNFLK"
     gene            complement(2365..2772)
                     /locus_tag="PLG49_04640"
     CDS             complement(2365..2772)
                     /locus_tag="PLG49_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16641.1"
                     /translation="MDKTKLGISVTLLAAAIYFLGAISILPAFILAGYVLLFEGDSWL
                     KKAAVRMAMVVITFGILSVFTGMLQDVFSVLNLTVGAILKKPVEVPLNLDKVLLTVIN
                     FTKNALLLVMGFKAFFFGTVQVPFIENSLDRNI"
     gene            complement(2943..3770)
                     /locus_tag="PLG49_04645"
     CDS             complement(2943..3770)
                     /locus_tag="PLG49_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ16642.1"
                     /translation="MFINNVLEIRIINQLNHIQKQQSILAYRLATGQRINSAADDAAG
                     LAISEKMRAQIRGLRQAQRNAADGISLIQTAEGAMNEIHAILQRMREISVQAANGTYT
                     DSDRRNLNEEFQQLKEAITGIATSTEFNTQSLLDGSKSHKGIRLQIGPNAGNSMEVTI
                     NDMRASGIGIENLDISTQEGAQEAIETLTKSIDYVSGERSKLGAIQNRLEHTIRHLEN
                     YEENLTAAESRIRDADMAATIMEYIKYQILSMTAQAILAQSMYLKRQQLLLLLSSIR"
     gene            complement(3908..5551)
                     /gene="gltX"
                     /locus_tag="PLG49_04650"
     CDS             complement(3908..5551)
                     /gene="gltX"
                     /locus_tag="PLG49_04650"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ16643.1"
                     /translation="MDYKKLADMLFPNITKTIDDFEKLFPERNLEEGAMVTRLAPSPT
                     GFIHLGNLYGAFVDERLARQSNGIFMLRIEDTDDKRKVEGAIEIIISSLEYFGLKFDE
                     GVSLDGEIGNYGPYYQSNRVEIYQTVAKYLVEQGRAYPCFCSEEELAEIRKQQEARKL
                     TPGYYGEWAKDRDLSLEEIKKRLDNGESFVLRFKSMGSEDRKFEIEDAIRGTLSMPEN
                     FQDIVLLKTNGIPTYHFAHVVDDHFMRVTHVVRGEEWLSTLPIHYELFKTLNWKLPIY
                     CHTAHMMKIDNGVKRKLSKRKDPEMGLSYYMELGYFPQAVREYLLTILNSNFEEWRMA
                     NPNSSIEEFNFSLNKMSTSGALFDLDKLNDVSKEVLVRIPVEEIFDFLLEWAKKYKNE
                     IEPLLLENKDSVIKLLSIGRDSNKPRKDLIYCEQIFDFISYYFDEYFKMVDPYPENIG
                     EEEAKIILKEYLKTYDHNDDQSQWFEKIRVISEQNGYAAKPKDYKKNPELYKGHVGDV
                     STVIRLAIVGRASSPDVWEIQQILGEEKVRERVQKAIGE"
     gene            complement(5853..6530)
                     /locus_tag="PLG49_04655"
     CDS             complement(5853..6530)
                     /locus_tag="PLG49_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOQ16644.1"
                     /translation="MNEVFNSPFFGISICILTYQIGRYLNRKLKSPLANPMLIAMILI
                     IAFLKIFNISYDQFNQGGQFISLFLAPATACLGVSVYKQLDVLKKNILPILLGCTVGS
                     LTSIVSVYCLCKLFKLDEKFMISLIPKSVTTPIGISITEQHGGMVPITVLAILVTGLI
                     GAIFAPLMIRIFRVKNPVAAGIAIGTSSHALGTSKAVEIGEIEGAMSSLAIGIAGLVT
                     VFFSIFL"
     gene            complement(6551..6823)
                     /locus_tag="PLG49_04660"
     CDS             complement(6551..6823)
                     /locus_tag="PLG49_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOQ16645.1"
                     /translation="MIIMLLLLIFKLIKPSQIKEQSDFLLKNMTILFIPAAVEIINYT
                     EYLKDNFLIFLLICLITTIITFVVSAYTVIFVMQWQEKKKKSNNIE"
     gene            6822..7031
                     /locus_tag="PLG49_04665"
     CDS             6822..7031
                     /locus_tag="PLG49_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16646.1"
                     /translation="MAIIVAGKGIHWLNPTLIKQIKKPALFAALNSRLFLLASHSLRH
                     SQYFNTDNSKSKFFKIKAIKHSIKI"
     gene            7160..7906
                     /locus_tag="PLG49_04670"
     CDS             7160..7906
                     /locus_tag="PLG49_04670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ16647.1"
                     /translation="MENSVLATVDGREIRQSDVIAFMQSLGQRGSQFNNPLGLQQLLE
                     EIIAQELLYSEALEQKFENEEDFLIAFNQIKKSLLIQYAVNKLMNSVSVTDEEVKEFF
                     ESNREMFSQPKTVSASHILISSEEEANKILDEINNNALDFAEAARKYSSCPSKEKGGA
                     LGEFSQGQMVPEFEKAAFSMKAGEISKPVKTQFGYHIIKVDKVNEAKSRNFEEVKEEV
                     KNQCLLYKQKQAYLKKQEELKEKYPVVINF"
     gene            8043..9074
                     /locus_tag="PLG49_04675"
     CDS             8043..9074
                     /locus_tag="PLG49_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16648.1"
                     /translation="MKIILKDEQLVIKHNFISLLFRIILAFLLALFFLFLAFRNLNQI
                     NIFFACSAIVVFILFLFLSFKKRLYQVTFDKYTKKVTITERNLFTKNSYDFPLDAIKG
                     INIDMSMENKGLFNVMVYNMSIDLLNEKIFIGSSLLQKRINALADSVNDFLDNPVQQI
                     NADHAIYNTEETIKYTASASSRFDKIKNNSLLGKEISQKIHPNSLELNIHTPLSTPIL
                     FFIICCFLGLFFSGAYNTPISDIKPFELLFFPLLSIIVIIINALFPSVSKVIIDRGNS
                     KVQIFSKGLKSKKYEEYPLEALKCIDTFTIEDKCHLIMEIHDKKIELGVLKRNETINA
                     IRDFMNMPL"
     gene            complement(9112..9654)
                     /locus_tag="PLG49_04680"
     CDS             complement(9112..9654)
                     /locus_tag="PLG49_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOQ16649.1"
                     /translation="MKKLLVLVDYQKDFVDGALGFEKAKELEQGIYEKIESYLKNGDK
                     VLFTYDTHYDNYLESREGKNLPVPHCIKNTEGHKLYGKINQFANADNILHLEKEGFGI
                     APKDMIKIASEIGEEIQEIEIVGLVTNICVISNAILFQSQYRNADIIVDARLCSSFDE
                     ALHKKTLDVMEGLQIKVINK"
     gene            9943..10410
                     /locus_tag="PLG49_04685"
     CDS             9943..10410
                     /locus_tag="PLG49_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOQ16650.1"
                     /translation="MPMNMSGIRLLIPLIFQKSKSPVLASEVLKEKHGICFAKSHLLA
                     ALLRCQKIPSGFCYQRLILDDVHYPYLVLHGLNGIYIQEFNQWIRLDARGNEEGGNAQ
                     FSLNHEQLAFPVRKELGEENIPIIFANPIDSVINALTSYKNRDEVWKNFPFNI"
     gene            10506..10946
                     /locus_tag="PLG49_04690"
     CDS             10506..10946
                     /locus_tag="PLG49_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ16651.1"
                     /translation="MIVKLEEHKNQKEIEVLIKYARMSPLIKKLEKIIKSVDKTIKCT
                     LDNSIVWVNASDIYYIEVVDKRTFVYGEKLVYETNYRLYQLLNELSDFGFVQISKSCI
                     LNIHYLDSIKPLINSRLQATLRNGEHIYVTRKYVSEIHKKLQGR"
     gene            10951..11346
                     /locus_tag="PLG49_04695"
     CDS             10951..11346
                     /locus_tag="PLG49_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16652.1"
                     /translation="MKEKIKNCLASTALSLILLCVLASIYEGRYLCINTVYQVFLANI
                     ILHLTLSLLARFESSYFLVEISVEFALFITIALIFAAIFDWYSSTPLWMLLFLCSLVF
                     LIANIIDLYKMSNKLTYINKQLKDLRENQ"
     gene            11368..12063
                     /locus_tag="PLG49_04700"
     CDS             11368..12063
                     /locus_tag="PLG49_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16653.1"
                     /translation="MEAILKAEKIEKIFKQEKILKGISLEIMPNSFTVLLGPSGSGKT
                     TLLNILSGLLKPSSGKVYYKDKAITEFSKNQLADWKRKNVSNIFQSYLLLNNLTVREN
                     IEIGMFSPDNGLPLDELSHLLEIHHLLDKFPSELSGGQQQRVAIARAVIKKPDILFCD
                     EVTGALDENNSKKVVALLHHLKQSLGLTVIFVTHNLQIAETADRVITIKDGLIHQDKI
                     NQNPISAYEMIWG"
     gene            12067..13785
                     /locus_tag="PLG49_04705"
     CDS             12067..13785
                     /locus_tag="PLG49_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ16654.1"
                     /translation="MKLLYKQMTKELSKNYLSLVLILLLVSFTSFIYFFVHFSVDGNI
                     AALQSLPSLKENEHLYLNALHSNAILARNMLTGSVILTSFVFYIFYHRFMKKNYKSLA
                     CLKALGFKDSVISSLFWAFTFILSVLGGLLGLIAGYFASDLLLKSGMKSYQMSGLVKR
                     IHISSFFIGIFIPAFFFCIISYLTYLPVATKETALLLSSQTDTSTNPFLLAAANKLAN
                     FYPGKNKLSVRLALRKPLALILIILAVTAFSVMFLLAYSLHLSSEKIYQSQILGHHYL
                     YDNLFDKPRLLEKPLPNSMPYLNTSGNIMTAKQAIQQEVIAFKNNSPLFELMDDKGNM
                     LPGPTSGEIVISPALRDLYDIKEGHQIELYIHNHCKTFTVSAIAFNAKLNSVYIHPSD
                     LEEMLSLPPHSYTGIWSIENISDAAKVISRYEKMDELEKNFVSNRISAVINQLVGCLI
                     GSILLFLALLLNFQDNTKDILILDLMGYQSGAIRKMLLDIYKPVIYFIFSLGLWPSMQ
                     IVRFILKSLSLQIGDYMPFQSNIFVIGGIFLLLNFIYFIVQWTFNLGINKIIKSDALY
                     QYSNNE"
     gene            complement(13854..14750)
                     /locus_tag="PLG49_04710"
     CDS             complement(13854..14750)
                     /locus_tag="PLG49_04710"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ16655.1"
                     /translation="MNIFVTGGTGFIGSYVVHELVSHGHQLTVLARNPNKVKGFINHP
                     QIEFIEGSLYDQDRIKEGLYGKDTCIHIALGWGDSPLEILERDTKASVYIFETAAELG
                     VGQIIYTSSTAAIGEFRPVMAEDIDCRPVDLYGATKAATEAYLLAIAKVKKIRCNIIR
                     PGYVFGNPVVEGAPMQPDDRFRKIVKAAKANEPIHLVKNDGTQFIWAGDLAKVYYRLL
                     SSQHNRQIFLGLSTQFISWEEIAQYAVGYLASESEIVLEDKGWKRDDAYFDVSLLKKE
                     FGYSFSAFEQIKRHVEYIANHE"
     gene            complement(14844..15359)
                     /locus_tag="PLG49_04715"
     CDS             complement(14844..15359)
                     /locus_tag="PLG49_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ16656.1"
                     /translation="MNMPCWIKIYLGQKNQLSDIYLKYLTGFFTVAYLILLGNLLFVS
                     GRYGAASRQVSLIPFKTIVSYLQSIFYSKNYVAIINIVGNIVLFVPIGFVFSIYIKNR
                     LLNFMILFMVPICIEILQFIMASGVTDIDDVILNIFGEMIGALLFWLLIFLQGKITGK
                     VSNDKRTVSEA"
     gene            complement(15374..15655)
                     /locus_tag="PLG49_04720"
     CDS             complement(15374..15655)
                     /locus_tag="PLG49_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16657.1"
                     /translation="MKEPVKKVISAILGLVLAAVNYYMGTLLDNFTGIFIVFFMNLII
                     GMTVLKVTKNYIYHLMITVCENIFLVWMNLFIILFRPAIFIGYEGILIF"
     gene            15821..16312
                     /locus_tag="PLG49_04725"
     CDS             15821..16312
                     /locus_tag="PLG49_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16658.1"
                     /translation="MQQNSFAKELTNWYKKSARKLPWREEYEPYKTWLSEIMLQQTQV
                     NTVIDYFNRFIAAYPDIYTLCQAKEEDIFKLWEGLGYYSRAKKILECAKTIVEEYNGV
                     FPKDLNQLKKLPGIGPYTAGAILSIAYNLPAPAVDGNVMRVLSRYQMLDLNIADTKKQ
                     ILL"
     gene            16332..16901
                     /locus_tag="PLG49_04730"
     CDS             16332..16901
                     /locus_tag="PLG49_04730"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ16659.1"
                     /translation="MTDAIPRDFNQALMDLGATICTPKQAKCSACPVKEGCKAFYHNK
                     VEKYPVKLKKNKKRLQKVAVILVKDQDKILIVKRPDSGLMANLWGFPVVEDIHGNAHE
                     NIEKFMKDAFGLEIFVQKTEEGPKHVFTHLVWEMQLFKCILQSTRQSTPKVLSEIQWV
                     KTEQLKEYAFPTAFKKIIMNEFDLKSLDL"
     gene            complement(16864..>17454)
                     /locus_tag="PLG49_04735"
     CDS             complement(16864..>17454)
                     /locus_tag="PLG49_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HOQ16660.1"
                     /translation="SGWIMAYEQILQKVFYNENIVVRVFASDSWLFSTDSKEFTIEMV
                     QFEDRKNFQIIPLEQVPPVVFSETMRDLDLAVSVAHAPGVDPEASLSTIEIRTVIIEE
                     IVNLLKLKNVKVAKNRAFIEGAYGEYTVHLGSGIVHKMAAGAICILPVHSEYRGKIFL
                     PFLDDDPKTVEIISKIILLSEDHKIKDPTILSQIRS"
     assembly_gap    17455..17554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17555..21971)
                     /locus_tag="PLG49_04740"
     CDS             complement(<17555..21971)
                     /locus_tag="PLG49_04740"
                     /inference="COORDINATES: protein motif:HMM:NF039515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HOQ16661.1"
                     /translation="MYLSNEKKHIVEGYLLEKIQNIHSDDECYDLVRALGKFVSCNFL
                     TYNKKCNELKAKFWAEERASIQELFSDINGKAYKALLLLLDKSSADYFIQLWNRSVLY
                     SYVLESPAKPFRSLRSSYVYFKKNMGKLIQWFYLTAKEFSLLHYLEGSTEAFENLPVI
                     SDMIAYETDKGNQEIISRIEDIIYHNNQAVVTKEIIQGLLKSTNEKAHKMLADLLMAA
                     NLQEGLRQTIVNCMAEASKEAFLYILKWIIDHQLTRFSSIAKGFNIWTGLSLDMNDSQ
                     RVEKAFKALYDCLSDSQKVMDYIQDEDEFYIYIGLWAAAFNETADLTPLVMELIEKEE
                     KNKNLVALYFLGQNSFSILKHQMACKLLRKNDYEILAWAINSLYSFNCYYDIYFDNDI
                     RFDLEGFKLYKHLNDGFKEKELFELLKQILETFPQKKLVFNSSLFSGILVELTDSEIL
                     NKMMYSILSEHEKEQVDYLITQRKKMSPEGRVFFIENFLAHSKEPVHKKVLLEACTDR
                     SEWVSNKAFELVNGLNLEAEDYKVIEGFLKYKSGYIRKKTILLLLKQNDQALLACIKG
                     LFQSGEEGKHSAAREMLSLLKKDTNRQTLYNECAAWMDENHEPDLEKERSLKEKIKNE
                     DINLASKKGFGLYDPTKELEIPLIAADENNDYKALFSAVLNKMISVLQAFSEEITMHR
                     NDMYEFIDFDGTKKRVVLGESSRLLPYYAGKRELDAFHLAEEMRNLKDKLALNANELI
                     VLNFYLRMFLASRFEEYDSWYLNLLNDYFQANLFKSLKEQISKISYLSLIHSYIDLLV
                     REIPKSEGFGMAKNFAAYLLKKIPLEQHQKEYTRKSLSDFALPANIKLFRKERFLAMS
                     PEINYWLFLIKDFIYDDKSFEEYFWIVYSYYKYGKYSDRLSLFLDDFVQAFEKGLIDE
                     NEICKEFLERRTAPDNISEFTNPILLRHRAWSEAFINIGNKVVETIVNIEIHRGEMHT
                     PVSHLAAKIQRCYGLEIMVSILLNLEKNAFVRGYNFLIGDFTRKQILSHLLKVCYPKK
                     EDTKEKLESLLKGKKVSDKRLIEIGMYAPQWLDLISHYLNMPDLKSAYWYFHAHVNSI
                     FLGEKQAFISRYSSIPIEDLKDGAFDFDWFKEVYTGLGEKNFKLVYDSAKYVAAGNLH
                     RRSQLFADAYLGKLSYEEAEKKIKESRNKDYLLCLGLIPVKDKEDLLRRYELMNQFKE
                     EAKKFGAQRQASEKRCAEVALLNLARNAGFPDANRLTWNMETEKMNAVKTYMNPYSLE
                     DLKLWLEIDENGQSQICCTKNGKALKNIPVRLKKDEYVEELKAVHDSLRKQFQRVRQH
                     FEEVMVKEEDLEAAELINLSKNPVIAPIIRHLVFLHDDQLGYFMDGNLVNDKGEVYIL
                     QEKDKVKIAHPVHFKKLNRWAEYQKDLFEKKIKQPFKQVFRELYLPYEDELGEKSSSY
                     RYAAYPIQRQKALALLQASGWIMAYEQILQKVFYNE"
     gene            complement(22017..22433)
                     /locus_tag="PLG49_04745"
     CDS             complement(22017..22433)
                     /locus_tag="PLG49_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16662.1"
                     /translation="MKFYIAVKKLGKQKEPITKKEFICSSSVTSLRDLMTFIIKEEVH
                     CYRHKAQGMEITPYLSKAEIEEKLAAGKVTFEEVYLSKEVHLDEALESAFLAFEDGLI
                     RVLVGEEEIKNLDAPLDLKDEEMVVFIRLMMLSGRF"
     gene            complement(22632..23504)
                     /locus_tag="PLG49_04750"
     CDS             complement(22632..23504)
                     /locus_tag="PLG49_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOQ16663.1"
                     /translation="MFTQYFGNYLLNKGYLTAEQLHDALEEIKDARLKLGVLAVNAGY
                     MTAEQTEEVNEAQKKYDKRFGELAIEMGYLTEEQLKELLSSQKYGHLLLSQVLVDKGI
                     MTLSEFEKAINQYKRDYSISDKEFEAIQSDDVNEIINKFIKFEGSKNEKLLKDYIALF
                     ARNLIRFIDRDAVIKEVSKLEVYDCQFAAKQDISGAIVLTTYIEADENAFIQFASKYA
                     EEELSSVDEMAEASVGEFLNLHNGIFLVNLSNQGIELKMTPQEVFKDIKVEKLFKVSA
                     ELSFGCVNILIQEH"
     gene            complement(23508..23888)
                     /locus_tag="PLG49_04755"
     CDS             complement(23508..23888)
                     /locus_tag="PLG49_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ16664.1"
                     /translation="MAKETFKVLICDDSILVRKKLKEALSALASFEFLEAGDGQTAID
                     VYKKERPDLVFMDLVIPVKDGITAVKEIKEFDNDALVVIASSSGTQENLKKAIKAGAV
                     EFIQKPWEQEQLSNVVKKFLKKGE"
     gene            complement(24058..26520)
                     /locus_tag="PLG49_04760"
     CDS             complement(24058..26520)
                     /locus_tag="PLG49_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOQ16665.1"
                     /translation="MLKKKFIALWLIFAMVFAFMPNAGLAGAVYAAEAEEQYLLVEPA
                     IKPSKAGALKVMEKNGVMTLCDASGNPIQLRGMSTHGLQWFPEIINSNAFAALSNDWG
                     CNVIRLAMYVGEGGYATNPSVKDKVIEGIKLAIENDMYVIIDWHVLTPGDPNAEVYSG
                     AMDFFDEISSLFPNHPYIIYELANEPNSTEPGVSNDAAGWQRVKAYAEPIIKMLREKG
                     NENLIIVGSPNWSQRPDLAADDPIDDDNVIYSVHFYTGTHKPSDDITDRSNVMSNAKY
                     AIENGVAIFASEWGTSEATGHNGPYLEEADEWLSFLNKNNISWVNWSLTNKNETSGAF
                     TPFDLGKTEATNLDPGADQVWDMKELSVSGEYVRARIKGIAYEPIDRTPKEEFSEIVW
                     DFEDGSVQGFGINGDSPIKEVSLSNENNALKISGLSVSSDVSEGNYWANVRLSADGTS
                     ARPDIYGAELLSMDVIADKPATVSIAAIPQSATHGWANPIRAIQVTADDFVEQENGKY
                     KATLSISKADSPNFEAIASDAEDSIMTNIILFVGTDGLDEIMLDNITVSGNRAVVEAP
                     IVHDPLGEPAFPSDFEDGTRQGWNWDPASGVKGEMKIKEAAGSKMLSWEVAYPEVKPA
                     DGWASAPRIILANINTTRGTNDYLTFDFYLEPVRANSGNLSIYLAFAPPSLGYWAQAA
                     KSFDISLKDLAQAEKTADGLYHYKVSFDLNDIADDKVLEADTLLRDITIVVADVESDF
                     AGRMYIDNIKFESKASAASLTESTKPVIQEKPSDNYYIVQPGDVLWKIAEKYGTTYEE
                     LAKINQLDNPHLIVSGQKLILP"
BASE COUNT         8574 a         5219 c         3858 g         8853 t
ORIGIN      
        1 attatatata aaatctttta aaatttctat actattttca caaattcaaa taatgttcaa
       61 catttattta tgtaaatttt acacgattat tatgaaataa tattaatttc taaatctttt
      121 agacaaaatt tttctttttt aatttatata ggggcattaa ctttcagttt gtgcttctac
      181 caatctgaca gctccgtatt tttcccttaa ttttggtttt tcaatttttc cggtcggatt
      241 gcgtggaact ttatcaaaaa taatttttcg tggacgctta taacggggca gggccttaca
      301 gaactgtata atatcctctt ccgtacaaat atgcccgtct tttaattcta taatagctgc
      361 tgctatttcc ccgagtctgt tatctggtaa gccaatcaca gcagcatctt taatagaatc
      421 atgggttcga aggaaatctt cgatttgaac agggtaaata ttttcccctc cactgatgat
      481 aacatctttt tttctgtcta ctaaatagat gaaaccatct tcatccattc tcgccatatc
      541 tcctgtatat aaccaaccac cctttaatac ttcttcagtt gctttaggat ttttataata
      601 acatttcata acaccaggac cttttacaat aagttccccg acttctcctg cctgcagagg
      661 attgccttta tcatctacaa tctttgcttc ccattgatat cccggtttcc ctatagcacc
      721 aactttatgt atattttcaa ttcctaagtg aacgcagcca ggacccacgg attcgctcag
      781 accatagttg gtatcatagt catgattagg aaaatacttt ttccacctgt gtattaaact
      841 tgggggaaca ggttgcgcac caatatgcat aagccgccac tgatccaatt tataatcttc
      901 cagttttact gctccacttt caatggcatc taaaagatct tgtgcccagg gtaccaaaag
      961 ccacacaata gtaatttgtt cttcagaaat cgtcttaagt atccactctg gtttaacccc
     1021 tctgagtaaa actgccttac tgccagctaa cagacttcca aaccaatgca ttttagcacc
     1081 tgtatgataa agagggggaa tacataaaaa gttatcttca cgggtttgat gatggtgtgc
     1141 tctttcagta taacatgcag atactaaact tccgtgggta tgtaaaatcg ctttgggaaa
     1201 tccagtggtt cctgaagaaa aatatatggc ggcatcatcc tcatcggata tgggaatatt
     1261 ggggggctgg gaacagcaat ttgcagttag ccggtcataa ctttctgcaa aagtgggacg
     1321 gttttctcct gcataaaaaa gcatttttac attaggaatc tgatcgtaaa tggcttctat
     1381 acgaccaata aattcaggtc cgaaaataac tgcaattgca tcagataaat ctaaacaata
     1441 ctttatttct tctgcagaat atcgaaaatt aaaaggtact gctatagcac ctgattttaa
     1501 aaccccaaaa tatattggaa gccattccag gcaattcatc aataaaatag caactttatc
     1561 tcctttttta attcctcttt ttattaataa attcgcaaaa cggtttgctt tttcatcaaa
     1621 tacacgccag gtcatatccc gtctgtactc acctaaagga ctgttttcta tcaattcaaa
     1681 ttctttccaa atcacattat gtttttcctg taaatccaga ttaatttctg ttaaacaaat
     1741 ttcatctcca tataatttcg catttctctc caaaaactca gtaatcggca tattttttcc
     1801 cccagttcat aaaaatattt aaatttgctt agattgttta ttattttcgt aaaaaatact
     1861 acacttatta tagattaaca aaaatcgtta atcaatagca aaagagacta aattcctttt
     1921 tggctcctta ttttgagaca aataaaaaag acttcttaaa atagaaatct tttattttaa
     1981 aaaattattt tttgttttat atataattaa aatcccactg aattctctgg gtggagacat
     2041 ggtctctaaa ctggattgta tacatgaaac agaatatttt atatctatta tttcaataga
     2101 atcccctttt tcttttaacc atctgttaac tgtcttttcc tgaagttctt cataacccaa
     2161 aaactctttt actaaaacca ttttatttcc ccctctaatg aaatgcttca taaaaaagga
     2221 ttactcaata ctaatatatg tatgtccact attgtttagt atatacatta atgtaataat
     2281 atggtaacat atcttttaaa aaaaatcaat ctgattaatc agttcttcta taaataaaaa
     2341 agtgtacaat tgttgtacac ttctttatat attcctgtcc agagagtttt ctataaaagg
     2401 aacttgaacc gtaccaaaga aaaatgcttt aaagcccata acaagcagta aggcattttt
     2461 tgtaaaatta ataacagtca aaagaacttt atctaggttt aaaggtacct cgacaggctt
     2521 ttttaaaatg gctccaacgg ttaaattgag aaccgaaaag acatcctgaa gcattcctgt
     2581 aaagacagat aatatcccaa aggtaataac aaccattgcc atcctgacgg ctgctttttt
     2641 taaccaactg tcaccttcga ataataaaac atacccagcc aaaataaatg caggtaaaat
     2701 gcttattgct cctaaaaaat aaatggcagc tgctaaaagc gtaactgaaa tacctaattt
     2761 tgttttatcc atattacctt cctcctatta aacttttatc aataaaatag agccataaaa
     2821 gctcaaaaaa aaagatactt ataaccaaca tgttatattg taccttatta ttaaaattat
     2881 ttcaatatat cataattttt cattttattt acaacttaat taaaaaaatg gatacgattt
     2941 atttagcgta tactggataa cagcaataac aactgctgtc tttttaaata catagactgg
     3001 gctaaaatgg cctgagctgt catggacaag atttgatatt tgatatactc catgatggtt
     3061 gcagccatgt ctgcatcccg gatacggctt tctgctgccg ttaagttttc ctcataattt
     3121 tctaaatgac gaattgtatg ttctaaacgg ttctgtatag cacctaattt agatctttct
     3181 ccggaaacat aatcaataga ttttgtcagg gtttctatgg cctcctgtgc accctcctgg
     3241 gttgaaatat caagattttc tattcctatc cccgaagccc tcatatcatt aatagtaact
     3301 tccatgctat tgcctgcatt aggaccaatc tgaagccgga tacctttatg actcttagaa
     3361 ccatccaaaa gagattgtgt attaaattca gtagaagttg caataccagt aatggcttct
     3421 tttaattgtt gaaattcttc atttaaattt ctcctgtcac tatctgtata agtaccatta
     3481 gccgcctgaa ctgaaatttc cctcattctc tgtagtatgg catgaatttc attcatagcc
     3541 ccttcagcag tctgaattaa ggaaatacca tctgcagcat tcctttgagc ttgtctcaat
     3601 ccacggattt gagctcgcat tttttcagat atagcaagac cggcagcatc atccgcagca
     3661 gaattaatcc gctgccccgt tgcaaggcgg taagcaagaa tactttgttg tttttgaata
     3721 tggttaagct ggttaataat ccggatttcc aatacattat taataaacat aaaatcaccc
     3781 tgaatattta tttttttatg ccagtctttg ctatcaaatg atatctgcat tctttataaa
     3841 tatatcggca aatattaaaa agtatttgag tgtaaactac aataaaaaag tctacactca
     3901 aatactttta ttcaccaatt gccttttgaa ctctttcacg gactttttct tctcctaata
     3961 tttgctgaat ttcccaaaca tccggagaag atgccctgcc gacaatagcc agacggatta
     4021 cagtgcttac atcacctaca tgtcctttat acagttcagg gtttttctta taatcttttg
     4081 gctttgctgc atatccgttt tgttcagaaa tgaccctgat tttttcaaac cattgacttt
     4141 gatcgtcatt gtgatcatag gtcttcaaat attctttaag aataattttt gcttcttctt
     4201 caccaatatt ttccgggtat ggatcaacca tcttaaagta ttcatcaaag taataactga
     4261 taaaatcaaa tatttgctca caataaatta aatctttgcg gggtttatta gaatctcttc
     4321 caatggataa aagctttata acagaatctt tattttcaag taaaaggggt tcaatttcat
     4381 ttttatattt ttttgcccat tctaataaga aatcgaatat ttcttcaaca ggtattctga
     4441 ctaatacttc tttgcttaca tcatttaact tatccaaatc aaataaggca ccagatgtac
     4501 tcattttatt taatgagaaa ttaaattctt ctatactgct attgggattt gccattctcc
     4561 attcttcaaa atttgaattc aaaatggtta aaaggtattc tcttacagcc tgtggaaaat
     4621 agcccaattc catataatag cttaagccca tttctggatc tttccgcttg gaaagttttc
     4681 ttttgacacc attatcaatt ttcatcatat gggctgtatg gcaatatatg ggcagtttcc
     4741 aattcagagt tttaaataat tcatagtgaa tcggaagggt tgagagccat tcttcccctc
     4801 ttacaacatg agtcacccgc atgaaatggt cgtctaccac atgggcaaag tgataagtag
     4861 gtattccatt tgtctttaaa agcacaatat cctgaaagtt ttcaggcatg gataaagttc
     4921 ctcttatggc atcttcaatt tcaaactttc tgtcttcaga acccattgat ttaaagcgaa
     4981 gtacaaagct ttcaccattg tctaaacgct tttttatttc ttcaaggctt aagtccctgt
     5041 ctttagccca ctcgccataa taacccggtg tcagcttcct ggcttcttgt tgctttctga
     5101 tttctgccaa ttcttcttca ctgcaaaaac aagggtaagc tcttccttgt tccacaaggt
     5161 atttagccac cgtctgatag atttctactc tgttactttg ataataagga ccataatttc
     5221 ctatttctcc atcaaggctg actccttcat caaattttaa accaaaatat tctaaagagg
     5281 aaataattat ttcaatagcc ccttcaactt ttctcttgtc atctgtatct tcaatacgaa
     5341 gcataaaaat accattgctt tgacgggcca ggcgctcgtc cacaaaagct ccgtaaaggt
     5401 ttccaagatg gataaagcct gttggacttg gtgccaaacg ggttaccata gcaccttctt
     5461 ccagatttct ttccggaaaa agcttttcaa aatcatcgat tgtcttcgtt atattgggaa
     5521 atagcatatc cgccagcttt ttataatcca tttttattgc ctccatttat tattttaaaa
     5581 aatattgacg gtcagtttgt ctaaaaaaca aataaccgcc atcttcattt ttttaagata
     5641 gataaagcta aaaactgcca ctgcaaagct tccattatcc ttgcaaatgg tcatacttaa
     5701 actattttac actattattc aaaaaatatc tatctatatt ttaatttatt tgttaaaaaa
     5761 ctcaaaactt gttgataaat taagacaaac ggcaatcaac ctatcatcca gatcataaaa
     5821 attggatgaa ctaaaaaatg atacagctcg ttttataaaa agattgaaaa gaaaacggtc
     5881 actaatcctg caatccctat agccagactg ctcattgccc cttcaatttc tcctatttct
     5941 acagcctttg aagttcccag agcatggctg gaagttccta tggcaatccc agctgccaca
     6001 gggtttttaa ctctgaaaat tcttatcata agcggtgcaa atatggcacc aataagccct
     6061 gtaaccagta tagccaaaac cgttatagga accatgcctc catgttgctc cgtaatactg
     6121 atacctatgg gcgtggtaac ggattttgga ataagcgaaa tcatgaattt ttcatccagc
     6181 ttaaataatt tacaaaggca ataaacactg acaatagaag tcaggctgcc cactgtacat
     6241 cccaaaagaa taggaagaat attttttttc agaacatcca gttgcttgta gactgacacg
     6301 ccaagacatg ctgtcgcagg agccagaaac aaggatataa actgtccccc ttgattaaat
     6361 tggtcataag agatattaaa tattttaaga aaagcaataa tcaaaatcat agcaataagc
     6421 atagggtttg caagaggact ttttaatttt ctgttaagat aacgtccaat ctgataggtc
     6481 aaaatacaga tgctaatccc aaaaaaagga gaattaaata cttcattcat tgtgaaaatc
     6541 ctttctatct ctattctatg ttattagatt ttttcttttt ttcttgccat tgcattacaa
     6601 aaattaccgt ataagccgat acaacaaagg ttataatggt tgtaatcaga caaattaata
     6661 aaaatatcaa gaaattgtct ttaaggtatt ccgtataatt gatgatttca accgctgccg
     6721 gaataaacaa aatcgtcata ttctttaaaa gaaaatctga ctgttcttta atctgtgatg
     6781 gctttatcag cttgaaaata agcaacaaaa gcatgattat catggcaatt atcgttgcag
     6841 gcaaaggaat ccattggctt aatcctacac ttataaaaca aataaaaaaa ccagccctgt
     6901 ttgcagcatt aaattcaaga ttattcctcc ttgctagtca ttctcttcgt catagtcaat
     6961 attttaacac cgataattca aaaagtaaat tttttaaaat caaagctata aagcattcta
     7021 taaaaattta accatttaat taaacaatcc agctgctttt gggaaaaaat taatcaatta
     7081 tattttttaa gttttacaat tagcagattt aatgatacac ttttaatgtt attaattttt
     7141 aaagatagga gataaaatta tggaaaatag tgttttagct accgttgatg gaagagaaat
     7201 cagacaatcc gatgtcattg cctttatgca gagtttaggg caaagaggtt cacagtttaa
     7261 taatccttta ggccttcaac aattattaga agaaattatt gcgcaagaat tgctttattc
     7321 tgaagctctg gaacaaaaat ttgaaaatga agaagacttt cttattgcct ttaatcaaat
     7381 aaaaaaatct ctgctgatac aatacgccgt caataaatta atgaattctg tatcggtaac
     7441 cgatgaagaa gttaaagaat tctttgaaag taaccgggaa atgttttccc agccaaaaac
     7501 cgtttctgcc agccatatat taatcagtag tgaagaagaa gcaaataaaa tcctggatga
     7561 gattaataat aatgccttgg attttgctga agcagccaga aaatattcat cttgtccttc
     7621 aaaagaaaaa ggtggagcct taggagaatt ctcacaaggt caaatggttc ccgaatttga
     7681 aaaagctgct ttttctatga aagctggtga aattagcaaa cctgtaaaaa ctcaatttgg
     7741 ctatcatatt attaaagttg ataaagtcaa tgaagcaaaa agcagaaatt ttgaagaagt
     7801 aaaagaggaa gtaaaaaatc aatgtctttt atataaacaa aaacaagctt atcttaaaaa
     7861 gcaggaagaa ttaaaagaaa aatatcctgt tgtcataaat ttttaaataa tctgtaagag
     7921 catttctata aagcttaagg aaatgctctt ttattgtata ataaaatttt aatagaatat
     7981 ttataattta caaaataatc atcttataat aaaattaaat caaaaataat agggggcatt
     8041 ccatgaaaat cattctaaaa gacgaacaac tcgtcataaa acacaatttt atctctttac
     8101 tcttcagaat tatcctggct tttttattag cactattttt tttatttctg gccttcagaa
     8161 atttaaacca gatcaatatt ttctttgcct gctcagcaat tgtagtattt attttattcc
     8221 tgtttttatc ctttaaaaaa agactgtacc aagtcacttt tgataaatat acgaaaaaag
     8281 ttacaattac tgaaagaaat ttatttacaa aaaacagtta tgatttcccg ttagatgcca
     8341 ttaagggaat aaatattgat atgtcaatgg aaaacaaagg actatttaat gtgatggttt
     8401 ataacatgtc tattgattta ttaaacgaaa aaatcttcat cggttcaagc ctcctgcaaa
     8461 aacgtataaa tgcgttagct gattctgtca atgatttttt agacaatccc gttcagcaaa
     8521 tcaatgccga tcatgcgatc tacaacacag aagaaacaat aaaatataca gcaagtgcat
     8581 catccagatt tgataaaata aaaaacaatt ccctcctggg aaaagaaata tctcaaaaaa
     8641 tccatcccaa cagcttagaa ttaaacatac atactccctt gtcaacaccc attttatttt
     8701 ttatcatttg ctgtttttta ggactttttt tctctggtgc ttataataca cctatttcag
     8761 atattaaacc ttttgaattg cttttttttc cgctgttatc tataatcgtc attatcatca
     8821 atgcactttt tccctctgtc tcaaaagtca ttattgacag gggaaattca aaagttcaaa
     8881 tcttttctaa agggctgaaa agcaaaaaat atgaagaata tcctttagaa gcacttaaat
     8941 gtatagatac ttttactatc gaagataaat gccatttaat catggaaatc catgataaaa
     9001 aaattgaatt aggcgttctt aaaagaaatg aaacaataaa tgccataaga gattttatga
     9061 atatgcccct ataagcaaaa agagcactta gtgctctttt ttaattattt tttatttgtt
     9121 aataactttt atctgcagtc cttccataac atctaaagtc tttttatgca gtgcttcatc
     9181 aaagctggaa caaagtctgg catcaacaat aatatcagca tttctatatt gggactggaa
     9241 taaaatcgca ttactaatca cacagatatt agtcaccaga cccacaattt caatttcctg
     9301 aatctcttcc cctatttcac tggcaatctt aatcatgtcc ttgggtgcta ttccaaaacc
     9361 ttctttttca agatgcagta tattatctgc atttgcaaac tgattgattt taccatacag
     9421 tttatggcct tcagtatttt taatacagtg aggaactggg agatttttac cttccctgct
     9481 ttctaaataa ttatcataat gggtatcata ggtaaataaa accttatccc cattttttaa
     9541 ataactttct attttttcat aaatcccctg ctccagttct tttgcctttt caaatcctaa
     9601 agccccatcc acgaaatcct tttgataatc tacaagcact aaaagttttt tcatagagca
     9661 atacctcctt tatcatctaa atataacttt ctaaattata acatatccgg cttataatat
     9721 cgaacagcaa aataaaaatg gattgataat aagggcctat caacgtaaaa tgattcatat
     9781 agcacaaaaa aaggggaatg gttatgaaat ttactctgga atcctcgaat ataaatgatt
     9841 atctgaaaaa aacgccaatc atcgattatg accatataga cataaaaaaa ttagctcaga
     9901 aaattgataa gcaatccggc agcaggctgg actatattaa aaatgcctat gaatatgtcc
     9961 gggataagat tgctcattcc gctgatattt cagaagagca agtcacctgt acttgcctca
    10021 gaggtactca aagaaaaaca cggtatttgt tttgcaaaat cacatttact ggctgccctg
    10081 ctccgctgtc agaaaatccc gtcaggcttt tgctatcaga gattaatctt agatgatgtc
    10141 cattatccct atctggttct tcacggcctg aatggcatat atattcagga attcaatcag
    10201 tggatccgtt tagatgcccg gggcaacgaa gaagggggca atgcccagtt ttctctgaac
    10261 catgaacaac tggcgtttcc tgtacgaaaa gaattaggtg aagaaaatat tcccatcatt
    10321 tttgccaatc ccattgacag tgttattaat gctttaacca gttataaaaa ccgggatgaa
    10381 gtatggaaga attttccttt taatatttaa aaagtcatac ttatgcatct cagtctctct
    10441 gatttgcatc ttaggtatca ggctcttgcc catcttttaa aaacatttat actggaggta
    10501 tatcaatgat tgtaaaatta gaagaacaca aaaatcagaa agaaatcgaa gtcctcatta
    10561 aatacgcacg catgagcccc ttaattaaaa aacttgaaaa aatcattaaa tctgtagata
    10621 aaaccattaa atgtaccctg gacaattcca ttgtctgggt caatgcttcg gatatttatt
    10681 acatagaagt ggtcgataaa agaacttttg tctatggcga aaaattagtc tatgaaacaa
    10741 attatcggct atatcagcta ttaaacgaac tttctgattt tggttttgta caaatcagca
    10801 agtcctgtat cttaaacatt cattatcttg acagtataaa gccattaata aacagccgct
    10861 tacaagccac tttgcgcaat ggagagcata tctatgtcac aagaaaatat gtctccgaaa
    10921 tacataaaaa attacagggg agataagaaa atgaaagaaa aaattaaaaa ctgtttggcc
    10981 agcacagccc tttccttaat actcttgtgc gtcctggcaa gtatatatga aggtcgttat
    11041 ttatgtatca atacggtgta tcaggttttt cttgccaaca tcatccttca cctgacatta
    11101 agccttctgg cacgttttga aagcagttat tttcttgttg aaatcagtgt agaatttgct
    11161 ttatttatta caattgccct tatttttgca gccatttttg actggtacag cagtacacct
    11221 ctctggatgc ttcttttctt atgcagtctg gtttttctga ttgccaacat catcgattta
    11281 tataaaatga gcaacaaatt gacctacatt aataaacaat taaaagattt aagggaaaac
    11341 caataaaaca ttataaggag aaaaacgatg gaagccattt taaaagctga aaagatagaa
    11401 aaaattttta aacaggaaaa aatattaaaa ggcataagct tagaaatcat gccaaattct
    11461 tttactgtct tacttggccc aagtggctcc ggaaaaacaa ctttactaaa cattttatct
    11521 ggtctgttaa aaccaagttc aggtaaagtc tactacaagg ataaagctat tacggaattc
    11581 tcaaaaaatc agttagctga ctggaagcgt aaaaacgtct caaatatttt tcaaagttat
    11641 ttattattaa ataatctgac cgtcagggaa aacattgaaa ttggtatgtt ttcaccagat
    11701 aatggcctgc ccctggatga attaagccat cttttagaaa ttcatcatct gttggataaa
    11761 tttccttctg aattatctgg tggtcagcag caaagggtgg ccattgcaag agctgtgatt
    11821 aaaaaaccgg atatactctt ttgtgatgaa gtaacaggtg ctctggatga aaacaatagt
    11881 aaaaaggttg tggccctttt acaccacctg aaacaaagcc ttggtcttac ggtaatcttt
    11941 gtaacacaca acctgcaaat tgctgaaacg gctgacaggg ttattaccat aaaagatggt
    12001 ctgatacatc aggataaaat caatcaaaat ccaatatctg cttatgagat gatttggggg
    12061 taaaaaatga aactcttata taagcaaatg acaaaagaac tatcaaagaa ttatttatct
    12121 cttgtcttga ttttactatt ggtatctttt acttctttta tttatttttt tgttcacttt
    12181 tctgttgatg gaaatatagc tgcccttcag tcccttccct cattaaaaga aaatgaacat
    12241 ctgtatctta atgcccttca ttccaatgcg attcttgccc gcaatatgct tacaggttct
    12301 gttattttaa caagctttgt tttttacata ttttatcatc gctttatgaa aaaaaattat
    12361 aaaagcttag cttgcctgaa ggctcttggt tttaaggatt ctgttatatc cagtttattt
    12421 tgggctttta cttttattct ttctgtcctt ggtggcttat tgggccttat tgcaggatat
    12481 tttgcttctg atcttttact gaagtcagga atgaaatctt accagatgag cggacttgta
    12541 aaaagaatac atataagcag tttctttatt ggtatcttta tacctgcgtt ctttttttgc
    12601 atcatcagtt atctgactta tcttcctgtg gctacgaaag aaacggccct gttattgtcc
    12661 agccagacag atacttcaac caatcctttt ttattggctg ctgccaataa actggccaat
    12721 ttctatcctg gtaaaaacaa actgtctgtc cgtcttgccc ttagaaaacc ccttgcactt
    12781 attctaatca ttcttgcagt cacggctttt tctgtcatgt tcctcttagc ctattccctg
    12841 cacttaagca gtgaaaaaat ttatcagtct caaatcttgg gacatcatta tttatatgat
    12901 aatctttttg ataaacctag gcttttagaa aagccactgc ccaacagcat gccgtattta
    12961 aatacttcag gaaatattat gacagcaaag caggcaatcc aacaagaagt tattgctttt
    13021 aaaaacaaca gccccttgtt tgaattaatg gatgacaaag gaaatatgct tcctggccca
    13081 acaagcggcg aaatagtcat aagtccggct ttaagagatt tatatgatat aaaagaaggt
    13141 catcaaattg agctgtatat ccataatcat tgcaaaactt ttactgtttc cgctattgcc
    13201 tttaatgcaa aactaaacag tgtttatatt catccttctg acctggaaga gatgctttct
    13261 ctgccgcctc acagctatac tggcatatgg agtatagaaa atatctctga cgccgcaaaa
    13321 gtcatctcca ggtacgaaaa aatggatgaa cttgaaaaaa actttgtttc aaaccgcatt
    13381 agtgcagtta tcaaccagct tgtcggctgt cttattggtt caatccttct tttcctcgcc
    13441 cttttattaa actttcagga caatacaaaa gatatcctca ttttagatct gatgggctac
    13501 cagtcaggcg caatccgtaa aatgcttttg gatatctata aacctgttat atactttatt
    13561 ttctctcttg gtctttggcc ttcgatgcaa atcgttcgct ttattctaaa atccctctcc
    13621 ctgcaaattg gagattatat gccttttcaa agcaatatct ttgtgattgg aggcatcttt
    13681 ttacttttaa attttatcta cttcatcgtt caatggacat ttaatctggg cattaataaa
    13741 atcatcaaaa gcgatgccct ttaccagtat agcaacaatg aataaatttt ttctaaaata
    13801 ccttacgcaa taaaaggtgc aggtcatttt ataaagacct gcaccttttt taatcattca
    13861 tgatttgcaa tatattctac atgtctttta atctgctcaa aagcactgaa actatagcca
    13921 aattcctttt ttaataaaga tacatcaaag taggcatcat ctcttttcca tcctttatct
    13981 tctaaaacaa tttcactttc tgaagcaaga taaccaacag catattgggc aatttcttcc
    14041 caactgataa attgtgtgga aagtcctaaa aagatttgcc ggttatgctg ggaactaagc
    14101 aagcgataat aaactttagc cagatcccct gcccatataa actgggtacc atcgttttta
    14161 accaaatgaa taggctcatt agcctttgcc gctttaacaa tctttctgaa gcggtcatct
    14221 ggctgcatgg gagcaccttc tacaacagga ttgccaaaaa cataaccagg tcttataata
    14281 ttgcagcgga tcttctttac tttggcaata gccagcaaat aagcttccgt tgcggctttc
    14341 gtcgcaccat ataaatcaac tggccggcag tcaatatcct cagccatcac tggtctgaat
    14401 tcaccaatcg cagctgtgga agaagtatag atgatttgcc cgacaccaag ttctgcagcc
    14461 gtttcaaaga tatataccga tgcttttgta tccctttcaa gtatttccag aggactgtct
    14521 ccccagccca aagcaatatg aatacaggta tctttgccgt atagtccttc ttttatccga
    14581 tcctgatcat ataaactgcc ttctataaac tctatctggg gatgattaat aaatcctttt
    14641 accttgttag gattcctggc taaaaccgtt agctgatggc catggcttac caattcatga
    14701 accacatatg aaccaataaa gcctgttcct cctgttacaa aaatattcat ccttcttcct
    14761 cccgtactta cttttttatt atcattaata cttgttcgaa attctatttt gtcaattggc
    14821 cctgaatcga ctctaagtcc tttttatgct tcagacactg ttctcttatc attgctcact
    14881 tttccagtta tttttccctg taaaaaaatc aacagccaaa ataaaagagc ccctatcatt
    14941 tctccaaaaa tattcaagat gacatcatca atatctgtta cccctgaagc cataataaac
    15001 tgcaaaattt caatgcatat gggaaccatg aataaaatca taaaatttaa aagtctattc
    15061 ttaatgtata ttgaaaatac aaaaccaatg ggaacaaata aaacgatatt acctacaatg
    15121 ttgataatgg ctacataatt cttagaataa aatatgcttt gtaaataaga aacaatggtt
    15181 ttaaaaggta tcagactcac ctgcctggaa gcagccccat atcgccctga aacaaataat
    15241 aaattgccaa gtaatattaa ataagccacc gtaaaaaagc cagttaaata ttttaggtaa
    15301 atgtctgaaa gctgattttt ctgccccaaa taaatcttaa tccagcaagg catattcatt
    15361 gcaactgtag ccgctaaaaa atcaatatgc cttcataacc aataaatatt gcaggccgga
    15421 acaagataat aaaaagattc atccatacta aaaagatatt ttcgcatacc gtaatcatta
    15481 aatgataaat ataatttttt gttaccttaa gaacagtcat cccaatgatt aaattcatga
    15541 agaaaacaat aaaaatcccc gtaaaattgt ccagcagagt acccatataa taattgacag
    15601 ctgctaatac aagtcccagt attgcggata ttactttttt aaccggctct ttcattgaaa
    15661 tcatccttta gtaaaagatt atttaaaata aaataatatt atttttattc taattgactt
    15721 ttctttctaa gtaaagaact gacaaattaa ctcaacgaat tgtgattcct ttgatttatg
    15781 ttatactaag tgtagacaaa aaagtatagg agaattcatc atgcaacaaa attcctttgc
    15841 aaaagaactt accaactggt ataaaaaatc tgccagaaaa ttaccctggc gtgaagaata
    15901 tgagccttat aaaacctggc tttcagaaat catgctacaa caaacccagg tgaatacagt
    15961 cattgattat tttaaccgct ttattgcagc ctatccggat atttataccc tctgtcaggc
    16021 aaaagaagag gatattttta aactttggga aggcttgggc tattattcca gagctaaaaa
    16081 aattctggaa tgcgcaaaaa cgattgtaga agaatataat ggtgtcttcc caaaagacct
    16141 gaatcaactt aaaaaactgc ctggcattgg accatatacc gctggtgcta tcttaagcat
    16201 tgcatataat ctaccagctc ctgctgtcga cggaaacgtc atgcgggtcc tttcaagata
    16261 tcagatgtta gaccttaata tcgcagatac aaaaaaacag atccttcttt gaaaaagaag
    16321 tcctgacctt aatgacagat gcaattccaa gagattttaa tcaggccttg atggacctgg
    16381 gtgcaacgat ttgtacacca aaacaggcga aatgctcagc gtgtcctgtt aaagaaggtt
    16441 gtaaagcttt ctaccacaat aaggtagaaa aatatccggt taaattaaaa aagaataaaa
    16501 aaagactaca aaaagttgca gtcatcctcg taaaagatca ggacaaaatc ctgattgtaa
    16561 aaagaccaga ctcaggactt atggcaaatc tctggggctt tcctgttgtg gaagatattc
    16621 atggaaatgc ccatgaaaat attgaaaagt ttatgaaaga cgcttttggt ctggaaatct
    16681 tcgttcaaaa aactgaggaa ggccctaaac atgtctttac ccatttggtt tgggaaatgc
    16741 agttgtttaa atgtattctt caaagtacaa gacaaagcac acccaaagtt ctttcagaaa
    16801 tccaatgggt aaaaacagaa caattaaaag aatatgcctt ccccactgct tttaaaaaaa
    16861 taattatgaa cgaatttgac ttaaaatcgt tggatcttta atcttatgat cctctgataa
    16921 aagaatgatt tttgaaataa tttcaacggt tttaggatca tcatccagga aaggaagaaa
    16981 aattttacct ctgtattcgg aatggacagg caaaatacaa atagcccctg cagccatttt
    17041 gtgaacaatc ccgcttccta aatgaaccgt atattctcca taagctcctt caataaatgc
    17101 acgatttttt gccactttaa cattttttaa ttttaataaa ttaacgattt cttctataat
    17161 taccgttcta atctctatag tggacaggct tgcttccggg tcaaccccag gggcatgggc
    17221 tacacttacg gccaaatcca agtccctcat cgtttctgaa aaaacaactg gtggaacttg
    17281 ctccagagga ataatttgaa aattttttct gtcttcaaat tgcaccattt ctatggtaaa
    17341 ttctttgctg tctgttgaaa aaagccagga atctgaagca aagacacgaa caacgatatt
    17401 ttcattataa aaaacttttt gcagtatttg ttcatacgcc ataatccaac cactnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttcat tataaaaaac tttttgcagt
    17581 atttgttcat acgccataat ccaaccactg gcctgtaata aggccaaagc tttttgccgt
    17641 tgaattggat aagccgcata tctgtaagaa ctgctttttt ctccaagttc atcttcataa
    17701 ggcagataaa gctccctgaa cacttgttta aaaggctgtt ttattttctt ttcaaataaa
    17761 tctttctggt attctgccca ccgatttaat tttttaaaat gcactgggtg ggctattttt
    17821 actttatctt tttcctgaag gatataaact tctcctttat cattgaccag attcccatcc
    17881 ataaaatatc ccagctgatc atcgtgtaaa aagaccaggt gtctgataat aggcgctatg
    17941 acaggatttt tacttaaatt tattaattct gctgcttcca aatcctcttc ttttaccatc
    18001 acctcttcaa aatgctgccg taccctttga aattgtttcc tcagagaatc atgcacagct
    18061 tttaattctt ccacatattc atcttttttt aaccgtaccg gaatattttt taaggccttt
    18121 ccattctttg tacaacagat ttgactttgc ccattttcat caatttctaa ccataatttc
    18181 aaatcctcaa gcgaatatgg attcatgtaa gtctttacgg cattcatctt ttctgtttcc
    18241 atattccatg ttaaacggtt tgcatcggga aaacctgcat tacgtgccaa attaagaagg
    18301 gcaacttctg cacatctttt ttcactggct tgtctttgag ctccaaattt tttagcttct
    18361 tctttaaatt gattcatcaa ttcatatcgt ctcaaaaggt cttccttatc tttaacagga
    18421 attaagccaa gacaaagaag ataatcctta tttctgcttt cctttatctt tttttctgcc
    18481 tcctcataac ttagttttcc taaataagca tctgcaaaaa gctgtgaccg gcggtgcaga
    18541 tttccagctg caacgtattt agcactgtca tataccagtt taaagttttt ttctcctaaa
    18601 cctgtataaa cttctttaaa ccagtcaaaa tcaaaagccc catcttttaa gtcttctata
    18661 ggaatagaag aatatcttga tatgaaagcc tgtttttctc ctaaaaaaat gctgtttaca
    18721 tgggcatgaa aataccagta ggccgacttt aagtctggca tatttaaata atgggaaatt
    18781 aaatccaacc attggggtgc atacatacca atctcaatca aacgtttatc actgactttt
    18841 ttccctttta ataaactttc gagtttttct ttagtatctt ccttttttgg atagcaaacc
    18901 tttaataaat gacttaaaat ctgtttcctg gtaaaatccc caataagaaa attatatcct
    18961 cttacaaaag catttttttc taaatttaat aaaatggaca ccataatttc taagccataa
    19021 catctttgga ttttagccgc taagtgactg accggtgtat gcatttctcc tctatgaatc
    19081 tcgatattta ctatcgtctc tacaacctta tttccgatat tgataaaagc ctcagaccag
    19141 gccctgtgcc ttagcaaaat gggatttgta aattctgaga tattgtcagg ggccgtacgc
    19201 ctttccagaa attctttaca aatttcattt tcgtcaataa gacctttttc gaatgcctga
    19261 acaaaatcat ccaagaataa agacaagcga tcagaatatt tgccatattt ataatagcta
    19321 tagacaatcc aaaaatattc ttcaaaactt ttatcatcat aaataaaatc ttttattaaa
    19381 aaaagccaat aattgatttc aggactcatt gccaagaatc tctctttacg aaacagcttg
    19441 atatttgctg gcaaagcaaa atccgataaa cttttcctag tgtattcttt ttgatgttgc
    19501 tctaaaggaa tcttttttaa taaataagca gcaaaattct tagccatacc aaagccttca
    19561 ctttttggta tttctcttac caataaatca atatagctgt gaataagcga taaatatgaa
    19621 atcttcgaaa tttgctcttt taaactttta aagagattgg cttgaaaata atcatttaac
    19681 agatttaaat accaggaatc atattcttca aacctggatg ccaaaaacat tctcagataa
    19741 aaattcagaa caatcagctc attggcattt aaagcaagct tatcttttaa atttctcatt
    19801 tcttcagcca ggtgaaaagc atccagttcc ctctttcctg cataatacgg aagtaaccgt
    19861 gaagattcac ccaaaacaac cctttttttt gtgccatcaa aatctataaa ctcatacata
    19921 tcatttcgat gcatagttat ttcttcgcta aaagcctgaa ggacacttat cattttattt
    19981 aaaactgcag aaaataaagc tttataatca ttattttcat ctgctgcaat aagagggatt
    20041 tctaattctt tcgtcgggtc ataaagacca aagcccttct ttgatgccag atttatatcc
    20101 tcatttttta tcttttcctt taatgatcgt tctttttcca aatcaggctc atgattttca
    20161 tccatccagg ctgcacattc gttataaagg gtctgtctgt ttgtatcttt tttcaaaagg
    20221 gataacattt ctcttgctgc agaatgctta ccttcttcac cgctttgaaa taaaccttta
    20281 atgcaggcaa gtaaagcctg gtcattttgt tttaataata aaagaatcgt ttttttcctt
    20341 atataccctg atttatattt aagaaaacct tcgatgacct tatagtcttc tgcttccaaa
    20401 ttcaagccgt tgaccaattc aaaagcttta ttgctgaccc attcgcttct gtctgtacat
    20461 gcttccagaa gaactttttt atgaacaggc tctttagaat gtgctaaaaa attttcaata
    20521 aaaaatactc tgccttcagg actcattttt ttcctctgag taatcagata atccacttgt
    20581 tctttttcat gctctgaaag aattgagtac atcatcttat tcaaaatttc tgaatctgtt
    20641 aattccacta aaatacctga aaataagctg gaattaaata ctaatttttt ctgaggaaaa
    20701 gtctctaaaa tctgctttaa tagttcaaaa agttcttttt ctttgaatcc gtcgtttaaa
    20761 tgcttatata atttaaatcc ttccaggtca aacctgatat cattatcaaa atatatatca
    20821 taataacaat taaaagaata aagactgttg attgcccagg ccagtatttc ataatcattt
    20881 tttcttaaaa gtttgcaagc catctgatgc tttaatatac tgaaagaatt ctgacctaaa
    20941 aagtataaag ccacgagatt tttatttttt tcttctttct caataagctc cattactaaa
    21001 ggagtaagat cagctgtttc attaaaggca gcagcccata agccaatata gatataaaac
    21061 tcatcttcat cctggatata atccattact ttctgggaat ccgataaaca gtcataaagc
    21121 gctttaaagg ctttttctac cctttgtgaa tcattcatat ccagacttaa acccgtccag
    21181 atgttaaagc cttttgcaat agaagaaaag cgggttagct gatgatcgat aatccatttt
    21241 aaaatataaa gaaaagcttc ttttgaggcc tctgccatgc aatttacaat cgtttgcctt
    21301 aatccttcct ggagattagc agccatgagt aaatcagcaa gcatcttatg agccttctca
    21361 ttcgtactct taagcaagcc ctgaatgatt tctttagtaa ctactgcctg attattatga
    21421 taaataatat cctcaatcct gcttattatc tcttgattac ctttatctgt ctcataagct
    21481 atcatatccg atatgaccgg taaattctca aaagcttctg ttgagccttc taaataatgt
    21541 aatagggaaa attcctttgc agtaagataa aaccattgaa taagcttccc catatttttt
    21601 ttgaaatata cataagaaga gcgcaggctt ctgaatggtt tagcaggaga ttccaggaca
    21661 taagaatata aaaccgatct gttccatagc tgaataaaat aatcggcaga agacttgtca
    21721 agaagtaaaa gcaaagcctt ataagccttt ccatttatat cagaaaaaag ttcctgtata
    21781 gatgctcgtt cttctgccca aaactttgcc tttaattcat tacatttttt attataggta
    21841 agaaaattac agctgacaaa tttacccaat gccctgacca aatcataaca ctcatcatca
    21901 gaatggatat tttgtatttt ttcaagaagg tatccttcaa cgatatgttt cttttcattt
    21961 gataaataca ttaaaaagcc cccttgtctc tcataaacca tctttctcat ttatatctaa
    22021 aatcttcctg aaagcatcat taaacgaata aatactacca tctcttcgtc tttcaaatcc
    22081 aaaggcgcat ccagattttt aatttcctct tctccgacca aaaccctaat cagaccatct
    22141 tcaaaagcta aaaaagcact ttccaatgcc tcatccaaat gaacttcctt tgaaagataa
    22201 acttcctcaa aagtcacctt tcctgctgcg agtttttctt ctatttctgc ttttgaaaga
    22261 taaggtgtaa tctccattcc ttgtgcttta tgtctgtagc agtgaacttc ctctttaatg
    22321 ataaaagtca tcaaatccct aagacttgta actgaagaac tgcatataaa ttctttcttt
    22381 gtaattggct ccttttgttt tcccagcttt ttaacagcaa tataaaattt catctcatct
    22441 ctcccaaaga tacaaatgat ttttattgct ttctcataaa actattcttt ataatttctt
    22501 aaatgctttt actttattat atatttttta tctaaatact caaatagata aatatcttct
    22561 gcaaatttat ttaaatacat aaaaataaac cttccttagg ctttatccta aagaaggctt
    22621 gttaaaagaa attaatgttc ttgaattaat atattaacac aaccaaaaga taattcagca
    22681 gatactttga aaagtttttc cacctttata tccttaaaaa cttcctgagg tgtcatctta
    22741 agctcaattc cctgattcga aaggttcacc aaaaaaatcc cgttgtgtaa atttaagaat
    22801 tctcccacag aagcttctgc catttcgtca acagaactta actcttcttc agcatattta
    22861 gaagcaaatt gaataaaagc attttcatct gcttcaatat atgtcgttaa tacaatagct
    22921 ccgctaatgt cttgtttggc agcaaattga caatcataaa cttctagctt actgacttct
    22981 tttataactg catccctgtc aataaagcgg attaaatttc ttgcaaataa agcaatatag
    23041 tctttgagta acttttcatt tttactgcct tcaaatttta taaatttatt aatgatttca
    23101 ttgacatcat cgctttggat ggcctcaaat tccttgtctg aaatactata gtctctttta
    23161 tactgattga ttgccttttc gaattctgaa agggtcataa tgcctttatc aaccaatacc
    23221 tggcttaaca gaagatgtcc atatttttga gaagacaaca attcctttaa ctgttcttcc
    23281 gttaaatagc ccatttcaat agccaattcc ccgaatctct tgtcatactt tttttgcgct
    23341 tcgttgacct cttctgtttg ttcagcagtc atataaccgg cattgactgc tagaacccct
    23401 aactttaacc gggcgtcctt aatttcttcc agggcatcat gtaattgctc tgctgtaaga
    23461 tagcctttat ttaataaata attaccaaaa tattgcgtaa acatgggcta ttctcctttc
    23521 ttcaaaaact ttttaaccac gttgctaagc tgttcttgtt cccagggctt ttgaataaac
    23581 tcaacagctc ccgccttaat cgcctttttt aaattctcct gtgttcctga agaagaagcg
    23641 ataaccacta aagcatcatt gtcaaattct tttatttctt ttacggcggt tattccatct
    23701 ttgacgggta taactaaatc cataaaaact aaatcaggtc tttctttttt ataaacgtct
    23761 attgctgtct gcccgtcccc tgcttccaga aactcaaaag aagccaatgc tgataaagcc
    23821 tctttaagtt ttttccttac taaaatggaa tcatcacaaa tgagaacttt aaacgtctcc
    23881 tttgccataa taaacccccc atcttattgt ttgaaaaaca acactattta tataattcta
    23941 cataaagtaa ctttttcctc tctgatatga gaattttcta cataaatttt attttttaaa
    24001 tacaaaaggg gggcagttat aaaagctgcc tcaaattttt gaggcagcct gtttgaatta
    24061 aggaagaatt aacttttgtc ccgatacgat taaatgagga ttatccaatt gattaatctt
    24121 tgccagttct tcataagttg ttccatactt ttcagctatt ttccaaagca catctccagg
    24181 ctgaacaata taatagttgt ctgaaggctt ttcctgtata acaggctttg tgctttctgt
    24241 taaacttgca gcagaagcct tagattcaaa tttaatatta tcaatataca ttctgcctgc
    24301 aaaatcgctt tctacatctg ctacaacaat agtaatatca cgtaataaag tatctgcttc
    24361 aagtacctta tcatctgcaa tatcgtttaa atcaaaggac actttataat gatataaacc
    24421 atccgctgtt ttttcagcct gagctaagtc ttttaaacta atatcaaagg acttagctgc
    24481 ttgtgcccaa tatccaagac taggcggagc aaaagctaaa tatattgaaa gatttccact
    24541 gtttgcacga acaggttcta aataaaagtc aaaagtcaaa taatcattcg ttccgcgggt
    24601 tgtgttaatg tttgccaata ttattctggg tgcagaagcc caaccgtcag caggttttac
    24661 ttcaggataa gctacttccc aactaagcat cttagaacct gcagcttctt taatcttcat
    24721 ttctccttta acaccagaag ctggatccca attccatccc tgacgggtac cgtcttcaaa
    24781 atcagatgga aaagcaggtt ctcctaatgg gtcatgcaca atgggcgctt ctacaactgc
    24841 cctatttcca ctaacggtaa tattgtccag cataatttca tccaaaccgt ctgttcctac
    24901 aaaaagaata atattggtca taatactgtc ttctgcgtca gaagcaatgg cttcaaagtt
    24961 aggtgaatct gcttttgaaa tggataaagt tgctttgtat ttaccatttt cttgttcaac
    25021 aaaatcatct gctgttacct gaatagcccg gatcggattt gcccagccat gagtagcgct
    25081 ttgtggaatg gccgcaatag ataccgtagc tggcttatcc gcaatcacat ccatagataa
    25141 cagttctgct ccataaatat ccggtcttgc actggttcca tctgcagaaa gacgtacatt
    25201 agcccagtaa tttccttctg aaacatcact gcttacactt aaacctgaaa tttttaaagc
    25261 attgttttca ttgcttaagc taacttcttt tattggactg tctccgttaa taccaaaacc
    25321 ttgcacgctt ccgtcttcaa agtcccatac aatttcactg aattcttctt taggcgttct
    25381 gtcaatgggt tcataagcaa taccttttat acgtgctctt acatattctc ctgaaacgct
    25441 aagctccttc atatcccaaa cctgatccgc gcccggatcc agattggtcg cttccgtttt
    25501 tcctaaatca aaaggtgtaa aagctccgga tgtttcattc ttattggtca gggaccagtt
    25561 cacccaactg atattgtttt tattcaaaaa gcttaaccat tcatcggctt cttccaaata
    25621 agggccatta tgtcctgttg cttcactggt tccccattca gaagcaaaaa tagccactcc
    25681 attttctata gcatattttg cattgctcat cacattactt ctgtctgtaa tatcgtctga
    25741 tggcttgtga gtacctgtat aaaaatgaac agaataaatg acattatcat catcaatagg
    25801 gtcatcagct gctaagtccg gacgttgact ccagttcggg cttcccacaa tgattaagtt
    25861 ttcatttccc ttttccctta acatttttat tataggttct gcatatgctt taaccctctg
    25921 ccagcctgca gcatcattgc ttactccagg ttctgtgcta ttgggttcgt tcgccaattc
    25981 ataaataata taaggatggt ttgggaagag gctggatatt tcatcaaaaa agtccattgc
    26041 cccactgtaa acttctgcat taggatcacc tggcgtaaga acatgccagt ctataataac
    26101 atacatatca ttttcaatag ctaatttaat gccttcaatc actttatctt taacagaagg
    26161 atttgtcgca tatcctcctt caccaacata catggcaaga cggataacat tacaacccca
    26221 gtcattagaa agggctgcaa aagcatttga attgataatt tcagggaacc attgtaaacc
    26281 atgagtactc attccacgaa gttgaatggg atttccggat gcatcacaaa gcgtcataac
    26341 cccatttttc tccataacct ttaatgctcc agcttttgat ggttttatgg ctggttccac
    26401 cagtaagtac tgctcttctg cttcagccgc ataaacagct ccagctaagc ctgcattcgg
    26461 catgaatgca aataccattg caaagattaa ccataatgca atgaattttt tctttaacac
    26521 ttaaaaagcc ccccttaaat aagattttaa gttaagtcta gtccaaaatt ttgaaataaa
    26581 aattttatga aagataatct tttt
//
LOCUS       DAOSUD010000024        26412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1207_length_26412_cov_25.728383, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000024 DAOSUD010000000
VERSION     DAOSUD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 26412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26412
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_26412_cov_25.728383"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLG49_04765"
     CDS             complement(<1..515)
                     /locus_tag="PLG49_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ16666.1"
                     /translation="MSLSRPYVTEVFNTVGKQQRKLPIYCVETSEKKVALSFDAAWGA
                     DDTDDLLAILDKHGVKATFFLVGDWVRKYPEEVKKIAAAGHDIGNHSNKHPHMGQMSK
                     EANIKEIMAAHEEVKKLTGIEMNLFRPPFGEYNNTVIEAAEACNYYTIQWDVDSLDWK
                     EYGVDPMIKQVL"
     gene            complement(645..1601)
                     /locus_tag="PLG49_04770"
     CDS             complement(645..1601)
                     /locus_tag="PLG49_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ16667.1"
                     /translation="MGLWQGYPEIHKELIKVENYMKEVIPSRKKILTDICLELIEAGG
                     KRLRPAFVIIGAKFGRFDEIKIIPLAASMELLHTATLVHDDIIDDSELRRGRVTVQAK
                     WGEDMAVYVGDYLFTKAFTILSDKMHYEYLNRIAHAFKTICEGEIDQYEIKYNMDVSV
                     TDYLKRIYRKTAVLFVMSILTGAYEGKCKRSTIHALGKFASFFGMAFQVYDDLLDYLS
                     SEEIEGKPIGNDIRQGIYTLPLLYALKDQKTGPEIKKFLSKKADISNEEIQQVIQLVK
                     ETRALDLTRELKEKFVKKALSALDNLSDSDYKNICIDLVKLL"
     gene            complement(1601..2122)
                     /locus_tag="PLG49_04775"
     CDS             complement(1601..2122)
                     /locus_tag="PLG49_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOQ16668.1"
                     /translation="MNKTKKMVFLSLLVGMAVVIHIIEGQIPVLFPGVKLGLANIISL
                     FALLVLGWKEALVIVVLRTIISSIYGGGFSVFLFSIVGGLLSNIVMIILYKFFKDGIS
                     ISSISVCGAIFHNIGQLFVAAVYIQDFKIYIYLPVLLISGVITGFFVGVSVKFLCYHF
                     KDMFAKERRRDDL"
     gene            complement(2141..2524)
                     /locus_tag="PLG49_04780"
     CDS             complement(2141..2524)
                     /locus_tag="PLG49_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOQ16669.1"
                     /translation="MKKGDKIVLMTVLALLVVSFLSVIVYRISYKGKALNAKIIQNQK
                     VLVEVDLEKIDEPKEWMVKGEEGHYNIIRVEKGRIRFIEANCPDLVCVHTGWLSQPGD
                     IAVCMPHNVLVKIEGEKEELDEISY"
     gene            complement(2536..3588)
                     /locus_tag="PLG49_04785"
     CDS             complement(2536..3588)
                     /locus_tag="PLG49_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOQ16670.1"
                     /translation="MKKVTNFVVLLIMIMIPLMACNNQSNKANVIQEEVFALGTIIEF
                     KIYDDDSKKAKEAIDKSIQRIREIEDKMTINKDKSELIEINNNAGINFIYVSPDTFFV
                     IKKASEYSKLSQGAFDLTIEPIVKLWGIGTDNARIPKQEEIDVLLDLINYEDLILDEE
                     SQSVMLRKKGQAIDLGAIAKGYAGDEVKRILTEHGIQTAFVNLGGNVVAIGTKVDGSP
                     WKIGIQNPLDERGSHVAVIEVKDKAVVTSGNYERYFIKDGKRYHHIIDPKTGYPAEAG
                     IISSTIVTDQSIDADALSTAVYVMGLEKGLKLIERMENVEAVIITEDNQIYVTAGLRE
                     DFKLSNPQFQLLENLN"
     gene            complement(3763..4221)
                     /locus_tag="PLG49_04790"
     CDS             complement(3763..4221)
                     /locus_tag="PLG49_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOQ16671.1"
                     /translation="MKKIILMCLSLALAVSVLSACNTGKQAEDGGDTTGGTQTEVSYK
                     DGSYKAEADSFDEHGWKPVITIEVKDGKISSVDYDEVNEAGASKETDEEYNKNMKDKV
                     GLGPAEAIPQLEKDLVEKQDVNAVDTVTGATGTTNNFKTMANKALEEAKN"
     gene            4541..6301
                     /locus_tag="PLG49_04795"
     CDS             4541..6301
                     /locus_tag="PLG49_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ16672.1"
                     /translation="MNKNSKKVFILGAGYGGIQAALTLQKKKKKSDNIEIFIIDKNPY
                     HTLLTEIHEVAANRINENGVTVYLKDIFQYTDVKVIQDYIKDIDFENQTLISENNSYS
                     YDFLIIGIGSEPHYYGIEGLKENGFSLWSHKDAVKIKNHILSMFDKAAKEPDPEKRKA
                     YLTFAVVGGGFTGTEMVGELALWVKDLANIYKVDLKEVRLLLIEALPKILSILDDSLI
                     HKAEKYLKNKLGVEILTNSPITKVNNDSFVINNEKTIKSHTLIWAGGVQANKCLIDID
                     LEKGKGERVCVDPYTRTKYKNVFAIGDFALFTTEDNQILPALVETALETGKYAAINVL
                     NLIRNQELEKAKPKLHGIMVSMGKNYAVANIMGIKLSGYPALLMKHMVNIHYLFGIGG
                     FELIGKYLKNEFWGVNHQRGFLHRHLDKVSPTFLLVFLRLYLGYMWLMSGLDKINSGW
                     FQYQMLAGSNVDTTTGATVMQLVSSHTPGWYAWLVDHLIIPNSMLFQKLVVLTEIGLG
                     LAFITGTFTFLAAIVSIGMNINFLLSTGLNDLWFLISSIPMLGGAGRAFGVDHYLMPY
                     LMRQWRHFVRKRRIKINLWK"
     gene            6411..6875
                     /locus_tag="PLG49_04800"
     CDS             6411..6875
                     /locus_tag="PLG49_04800"
                     /inference="COORDINATES: protein motif:HMM:NF040162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HOQ16673.1"
                     /translation="MISLNITDVKKTMDELLKGNLFDEFELRTAEVHTFAKFQIQGNL
                     NKAFLTEDEKDLYERNFVLWKEIKSHIFNIIKGKKPPTYLKIVFSAAIDKIPDFASFD
                     ISALFINLIYSQGKLSCTNGIAFENFSLDKTIEQEWDQYVRDFFKKANIYVE"
     gene            complement(6877..7257)
                     /locus_tag="PLG49_04805"
     CDS             complement(6877..7257)
                     /locus_tag="PLG49_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ16674.1"
                     /translation="MKVRDIMNDTFYMVMPQDTLEKTLSIMETKKVNGLPVVDENKLL
                     VGFIVKADIYRFLSTTGHYEACPVDWAMSKDVITAMPDESIKEAARRLRQHNIIGMPV
                     VEQGRVIGLISIENIVDYFLTLEI"
     gene            7429..9315
                     /gene="htpG"
                     /locus_tag="PLG49_04810"
     CDS             7429..9315
                     /gene="htpG"
                     /locus_tag="PLG49_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOQ16675.1"
                     /translation="MARETGNLSIHSENIFPIIKKWLYSDSDIFIRELVSNSSDAILK
                     LKKLVTLGEATVPENTEYEIEVILDEKNKTIKVSDNGIGMTADEVKKYINQIAFSGAE
                     DFLKKYKDKMDKDQIIGHFGLGFYSAFMVADKVQIDTLSYQENASPVRWICAGGTEYE
                     MEESDRNTRGTTVTLYIGDNGKEFLNEFKLREILEKYCAFMPVPIYFEKAEHKKKKNE
                     EDKKEERKPINDTKPLWLKKPNDCTEEEYKDFYKKVFMDFNEPLFWIHLNMDYPFNLK
                     GILYFPKLRNEFETIEGKIKLYNNQVFVADNIKEVIPEFLLLLKGAIDCPDLPLNVSR
                     SFLQNDAYVKKISNYIAKKVADKLVSLYKSKKEDYIKYWDDIHPFVKFGAIKEEKFYD
                     KVKDTILYKSINGNYLTLKEYLEKNESNHKNKVFYVTDERQQAQYIKLFKDNNMDAVI
                     LNSTIDAPFMSHIEMRESDIKFYRIDADLSENLKKDEDNKGDEKAKDFAQKLEKIFKD
                     NLNKPDLKVQVESLKSEDISAMILLSEETRRLEEMSKMFGGLEMPGLFTKEQTLVLNK
                     NNQLIQMIYDKSEDENEKELLNILCNQIYDLALLSHQTLEAEAMTQFIERTNKIMAIL
                     AEKN"
     gene            complement(9366..13073)
                     /gene="addA"
                     /locus_tag="PLG49_04815"
     CDS             complement(9366..13073)
                     /gene="addA"
                     /locus_tag="PLG49_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HOQ16676.1"
                     /translation="MNWTKEQQEAIYTRGCDLLVAAAAGSGKTAVLVERIINIITDKD
                     NPVDIDRLLIVTFTNAAAAEMRERIGEALSKKLEEEPRNFHLQKQLALLHRASISTIH
                     AFCLNVIKSNFHLLDIEPSFRIADEGEILLIKTDVLEELFENLYQREDEGFLTLVESY
                     GGKKEDTKLKEMLLDIYNFIESHPWPKDWLRQKVESFNPEQIDFKNSIWIKMIKEQIL
                     EESKELLEIAKQAIKLCMEPAGPKKFIDALNDDISHIEKIISLCEGDIEELYNYIQQI
                     SLKPLNERSKDIDENIKTEVKMLRESIREGIQKGFKDKLLFKSPKEMEKDLHKLYPVM
                     KALENVLNEFENRYQAVKKDKGIIDFNDIEHYCLNILLDEKSSMEQIIPSAVAIELQK
                     RYKEILIDEYQDSNLVQETILELISGKYAGMPNRFMVGDVKQSIYRFRLAKPDLFIEK
                     YNSFSTEATGKEQRIDLFKNFRSRKNILDGVNFLFKQLMTPGLGEIEYDEKAALNYAA
                     NYPDEEGLNFGGPIELHLIESELTEKEDNIDEELEALTSIELEAKIVARRIKRLVLED
                     NFYIFDKKLKAYRKVDYKDIVILLRTTETWAHAFTEELTKEGIPAYADVSSGYFDAIE
                     VKTILALLHIIDNPRQDIPLIAVLRSPIVGVSCDDLVEIRTSFSYGDFYESLKRYIED
                     DFQETELSKRLKNFINNLERWRSLAAHIPIDELLWILYMESNYYNYLGAMPGGEQRQA
                     NLRMLRDKAANFEATSYKGLFNFIRVIERMQTGGKGDMGAAKIVGENENIVRIMSIHK
                     SKGLEFPVVIVAGLGKQFNLKDLNNPVLLHQDLGLGPNYVDYEKRLSYETVAKVAIKR
                     KITLETLSEEMRILYVALTRAREKLILTGSVKSIESGAKKWVRQVASEKEELSPYILS
                     KSRTYLDWIGMALARHKDGEKIKSWGNSFGSSVQSLYEDTSSWELIKWSKRDIHIQDE
                     SKAVKKEEVLNRLLNWDYSKTYSPEREEIFRRLSWKYPYEAATKISVKLSISEIKSQY
                     EENHRDYPMDSVFEDLQPRQPKFIEEGQVFTGAERGTIIHKVMRHLDFGAKDYKAIEE
                     QLVKMELKGLLTEEERKAVYIPSLLNFCKSGLAERIRKSSLVKRETPFILSLDAREIY
                     KGLNSEEEIFIQGIIDCFFEEEDGIVLVDYKTDFILNKDNPEKEIEKIMDKYRVQINI
                     YTKAIEEITGLKVKEKCLYLFGISRAVFYEE"
     gene            complement(13092..13598)
                     /locus_tag="PLG49_04820"
     CDS             complement(13092..13598)
                     /locus_tag="PLG49_04820"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOQ16677.1"
                     /translation="MKENIVLIGMPGAGKSTIGVLLAKALNMSFLDTDLLIQKKENKL
                     LQEIIEEKGISEFLDIEKNVILEVKVSNTVIATGGSVILRDEAMKHLKKTGFVFYLNL
                     PYKVIQRRLYNIKTRGIAMDKNKGLLDVYKERVPLYEKYADKIIQCAGRNVEDIVTEM
                     TALWKSVQ"
     gene            complement(13614..14798)
                     /locus_tag="PLG49_04825"
     CDS             complement(13614..14798)
                     /locus_tag="PLG49_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOQ16678.1"
                     /translation="MTKKCYYDDPYTSVWESEIVELIPKDDKYLVVLDKTYFYPEGGG
                     QPSDTGTIEDIYVSYVFEEDGKIYHLMEKAPKEKKVQCKIDFEKRFTNMQQHTGEHLL
                     AATFYKYYQGVSNAFHMGEDYTSIDISLSEVTPKMVKEIEDKVNEIIYKNVEVKTYLK
                     DIEEAKKLPLRKQPKVDEDIRIVEIDGVDFCPCCGTHVKRTGEVGILKIIKTEKSKGA
                     TRIYFQCGRRAFEDYSKKHDVISTLNETFSANENTLLKKVEKYLEDVKEKENQIKELK
                     KELVLYEAQKLLKNAEESVIYGQYDNKSMDEILFISKQVLQMGKYIFIGLSLKEMKLF
                     IAHNTDFLIHCGNLIKSEVSKFGGKGGGSPIQAQAVFENQQDLLKCGQALLAICKSLE
                     YA"
     gene            complement(14808..18248)
                     /gene="addB"
                     /locus_tag="PLG49_04830"
     CDS             complement(14808..18248)
                     /gene="addB"
                     /locus_tag="PLG49_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HOQ16679.1"
                     /translation="MSVRYILGRSGSGKTHYIFNDIKDKLREKEDIARILIVPEQFTF
                     QTQKDLINFLNQKGIMAVEILSFQRLAFRIFEELGPPREKFLGELGRNMVLSKVIDDI
                     SEELKIYHKSVKQPGFMQNLGDMICEFQQYDVRPTDLLEKKEFIKDRPILEAKINDLF
                     LIYQNFNNYIKEKYITSQETLDLLAQKIGDSNFLENAYIWIDGFYGFTPQQYRILTGL
                     FKKAKEVSISLTLDPRGDLYQPFDETDPFYESRRTMDKLNKLTAFYNIALEEPVKLYN
                     EKEPGINNKELYHLEKEYFNYPVQVYENKTEYIKLFVASNPYSELEHTARLILEFVRD
                     KGYRYREIAVVTGKISQYQKLIEAIFKEYNIPFFIDEKKDILSHPLIELIRAALEILN
                     SNWSYESVFRYLKTGFTGIAMEDVDLLENYVLAYGIKGISKWTKEDWKIIKEDFEDEL
                     QKKYEESLLERINQIRNIFIEPLIKLSQKLKNKKNHTFASITKALYEFLEDLGVRERL
                     AEYISHFKEDNKLSLVNENLQIWDFVVELFDKMVEILGEEPVSLKQYPRVLDAGFEQC
                     KMGLIPPALDQIVVADLERSRLPRIKALFVIGINDGIIPANKEELRLFSDEERRTMEE
                     LGVELAPDSRRKAFEEQFLIYSGLTKPEEYLHLSFSLGDVEGKALRPSVLISRIKKIF
                     PKLSQESDLIMSEESDFISSPGATFSYLGQALRELLDADDFSDVWKDVLSWYVSQEDW
                     KDKVNITIRGLFHNNQEYYLKKETVRKLYKDKIYTSVSRLEKFAACPFGYFVQYGLKA
                     KERKIYQLSLPDLGILFHSALDIFSKKLLNSGLSWRDLNDGLRDQMVEETINEIAPKL
                     GNEILLSTEKNKYLIRRIKRITKRAVWALTEHVKRGNFKPAEFEIGFGENHPLPPIVI
                     ELSNGEKLILTGRIDRVDILDEEDKIFVKIIDYKSGSQSFNLADIFYGMQMQLILYLD
                     AFLESGQILYGKKIFPAGAFYFRIDDPIISSAGEMTEEELQKLLLKELKLSGIVLEDE
                     EIIRKMDNTIEKSSDIIPVEMTKSGIGKKSNTATMEQFDSLREYVRKMAVEIGEEIIK
                     GNVKISPYKSDKGSSCQYCLYKSICQFDTLLEDNHYKYLHKLSKDEIWKKIEESIEKE
                     "
     gene            complement(18277..19428)
                     /locus_tag="PLG49_04835"
     CDS             complement(18277..19428)
                     /locus_tag="PLG49_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ16680.1"
                     /translation="MLKNLKLVLLLICLISLISPACNNTSHSNNDKTNREEMITEGKK
                     EENLAEDDEKEENTEEDQEQAKENEEKNEIVEIDYNKIRPNELGHIMIIMYHGLVEEN
                     PPSPYQRTIEGFKKDLEYLYEHGYRAISMRDFIDNNISVEAGCTPVIITFDDGISSSF
                     SLIEESGVLKAKPDCAVDIINKFCEEHPDFGRHAVFYINGDNDPFAGAGTTKERFKYL
                     IDHGYEVYNHTYSHAHLSQLSANEIQEQVGKLDQMIKEALPGYVVDSLAYPFGERPKE
                     ELRHLIEKGVYNGQEYHYKIALREGPSGPFVPVNHVKFEPFNVPRVRGSEGEEWDLWW
                     FFDYYDKHPEYRYISDGNPDRISIPKEMEAYLNKDSLRDKELYVYTIEE"
     gene            19622..20605
                     /locus_tag="PLG49_04840"
     CDS             19622..20605
                     /locus_tag="PLG49_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ16681.1"
                     /translation="MGLLRKKKQTNNQTQNQQVLDEYAAKVNEALVPVKFSAKELQDI
                     LAKIYTPTQTISSLLVQPETVQTDNLQELSIKYQNLESEIKQISRNIDSMKEIAENAV
                     NTAKDGQVFLRESIDKINKLRNTIERTNQTISSLNKSSSEIGNISQIITSISTQTNLL
                     ALNASIEAARAGEHGKGFAVVAEEVRKLAEQSTKSAEDITNLISGLQQQTTEISEIMS
                     TSASEVTETVSAVNKAGNAFEIISQFVETILTQSNAINNTLELIISSMKSFATAVENS
                     SHTTANDILLEDIKRNNQAQIQALGEFKQAYDNLINKISTLQILAQAYNDN"
     gene            complement(20631..22592)
                     /locus_tag="PLG49_04845"
     CDS             complement(20631..22592)
                     /locus_tag="PLG49_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOQ16682.1"
                     /translation="MDIKLKSNIKLSIFAFVILLLFSFGLTNTLLLKSNLSDRVKKSY
                     FDTDEFSREVEKFINLFSITQLTYEEYTEIYTEDLDDNLTKSYVKNEMEPEKTLFESA
                     EKNFKYYIKEPTGKVYTNLEENESFETVKASSYFIKSFPYHTKGNDPFISVNRFFITN
                     GLTGFIAVPKMDGAHTIIEENMYDYEDTRMQFFTFFGIGAVVLVISLFLGLIIIKKYG
                     KELIQLVEPLKKFYVFPIDIKILIFLVNLLILFGCQPVVFYQAGYRFYYGGSVDLLLY
                     TVFLIAFIIQMLFLFQAFSSSQQIKTEWSKSFVIRAKNNIADSFIVKSSVFKIFFILG
                     LVSFLGIIVGLVLPLIEPVDLLVLVPVGLIPTLAILLYIFSKLAYFNRIVRYSDDIIL
                     GRANADLVVKGKDVLSRLAGNLNRMRQGIDYSHKAQLKSERLKTELITNVSHDLRTPL
                     TSIMNYSELLKKEDLSEEKRKEYIDIIEKKSKRLKVLIDDLFEVSKMASGNIELNIEK
                     VDIVQLMNQALGEYDEKINNSSLNFKVSSSSPHIYAMVDGRKMWRVFDNIISNILKYS
                     LENTRVYISIKETDNYVMISFKNISKYELEENIEELLERFKRGDKSRNTEGSGLGLAI
                     AQSIVDNHGGKLEIELDGDLFKTIVTLKK"
     gene            complement(22570..23262)
                     /locus_tag="PLG49_04850"
     CDS             complement(22570..23262)
                     /locus_tag="PLG49_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ16683.1"
                     /translation="MENYKVLVVDDEKEIRDAIEIYLRNEGITVFHAKDGIEALDILN
                     KEEIHLILMDIMMPRQDGISTTFKIREEKNIPIIMLSAKTEDTDKILGLQVGADDYVT
                     KPFNPVELIARVKAQLRRYMKLGVYEGLKKVIDINGLILNQETREVIANGEKVRLTST
                     EYKILELLMLNAPRVFSIDEIYERVWNEPSYNSENTVAVHIRKIREKIEINPSEPRYL
                     KVVWGIGYKIEK"
     gene            complement(23458..24672)
                     /locus_tag="PLG49_04855"
     CDS             complement(23458..24672)
                     /locus_tag="PLG49_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ16684.1"
                     /translation="MTDINDNVFTAECIVSVDEYVSDKPVLSMPVASLPSGTYVGPQS
                     LYLETDNRLNTIYYTTDGSEPNSGSNVYRNAIYIDKDVTIKAIAIRNYSKSDVLVLNY
                     KILPQEDNEIIQPKEDRQDSGRTIKSAVEAVEEIKEIINKLTKDKKKTIAKEIEDALC
                     YTSLGENIVSENVLGEFTKTIFSMEQVQIIVNTPELRTLFGIDESKLSKQVVLTPVDE
                     PQFTDVTEKHWAREPIKRASQMGLVAGLPDGRFAPAAPLKVADTFTFLDRLFVLNQVT
                     EMKLPRTTVEKYITNKEHWAFSSMASIASKLSEETLETISNLGPDGDISRELLCQIIY
                     EITDGKLEPVRDEIAFADIENSPYKEAINYCVRVGLISGVDSCHMAPKKDLTRAELMT
                     ILIRLHEKLRQR"
     gene            complement(24669..25562)
                     /locus_tag="PLG49_04860"
     CDS             complement(24669..25562)
                     /locus_tag="PLG49_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16685.1"
                     /translation="MNYKRRAVSICLSILMFLMTFSQLIVYAADLGSEFIIISCGAKS
                     NNKEFHLDFDRKIDIPLQSIPFEEYVQYSVDDGSYQDLTLEDYVFVNNNRMDIFLKNP
                     LEGKKVKFKIESFSIIDEYNSYNTNEIISPEIDLSQIGEIKNLPSALSLYENETIFLE
                     IETTEYAATSDIKWELESDEEDKLIIIPNGFVCQVIAKRTGMYTVSVYDIRNPKSKKT
                     CNITVKAHDEKAVSIQSMTLDDNRIEMKVGNVRRLIPRVYPEEANIKNIVYYVEDPAI
                     ISVINNELIAEKKVVRRSALK"
     gene            complement(25953..>26412)
                     /locus_tag="PLG49_04865"
     CDS             complement(25953..>26412)
                     /locus_tag="PLG49_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16686.1"
                     /translation="VSRYGSTFLLSHLILQLTLYFIDSRNFAAGTYDLYLMSYDVNGN
                     LLEITPKEKLNIENPFNVIRVTPQKIKVSYINGNSYINIVGNGFSDATRVKIIDGNKE
                     EEITPKVISHNYMRIPIPNLIKNTTDDKTVIIRATDGMNQADIKIEVTGK"
BASE COUNT         8275 a         5161 c         3694 g         9282 t
ORIGIN      
        1 agaacttgtt ttatcatagg gtctacccca tattccttcc agtcaagaga atcaacatcc
       61 cattgaatcg tgtaatagtt acaggcctcc gctgcttcta ttactgtatt gttatattct
      121 ccaaaaggtg gtctgaataa attcatttct atgcctgtta acttcttaac ctcttcatgc
      181 gccgccatta tttcttttat gtttgcttct tttgacattt gccccatatg agggtgctta
      241 ttggagtgat tccctatatc atggccagca gcagcaatct ttttcacttc ttctggatac
      301 tttcttaccc aatcccccac taaaaagaag gtagctttta caccatgctt gtcgagtata
      361 gccaataaat catccgtgtc atctgcgccc caggcagcat cgaacgagag ggcgactttt
      421 ttctcagaag tttctacgca atatattgga agcttccttt gctgttttcc aactgtatta
      481 aaaacttccg ttacataagg tcttgaaaga ctcatgcaaa aaatgattaa caggagagcc
      541 cagataacct tagtaactcc ttttagatta ataacatata ttttcatagg aatcccccct
      601 ctaaatatta ctattcagag gggggatggc catattccat tcttttataa caattttact
      661 aaatcaatac aaatattttt ataatcactg tcagaaagat tatccagggc agataatgct
      721 ttttttacaa acttttcttt taactctctt gttagatcta aggctcttgt ttcctttacc
      781 agttgtataa cctgctggat ttcttcatta cttatatctg cttttttact caggaatttc
      841 tttatttccg gtcctgtttt ttgatctttt aaggcatata ataagggcaa tgtgtatatc
      901 ccttgccgga tgtcatttcc aataggtttc ccttctattt cttcacttga aagataatct
      961 agaagatcat cataaacctg aaaggccatt ccaaaaaaag aagcaaattt ccccaaagca
     1021 tgtatcgtgc ttcttttgca tttaccctca taggccccgg ttaaaatact cattacaaac
     1081 aatacagcag tttttctata aattcttttt aaataatccg tcactgaaac atccatgtta
     1141 tattttattt cgtattggtc aatttctcct tcacaaatgg tcttaaacgc atgagcaatt
     1201 ctgtttaaat attcatagtg cattttatcg gataaaatag taaatgcttt tgtgaataaa
     1261 taatcgccta cataaactgc catgtcttct ccccattttg cctgaaccgt tactctacct
     1321 cttcttaact cagaatcatc aataatgtcg tcatgaacaa gggtggccgt atggagaagt
     1381 tccatggagg cagctaaagg aataatcttt atttcatcaa aacgcccgaa ttttgctcct
     1441 ataataacaa aagcaggtct taatcttttt ccgccagctt ctattaattc taaacaaata
     1501 tctgttaaaa tcttttttct tgaaggaata acttctttca tataattttc tactttaatg
     1561 agttccttat gtatttccgg atatccttgc cataagccca ttataaatcg tcccttcttc
     1621 gttctttagc aaacatatct ttaaaatgat agcagagaaa ttttacactg actccaacaa
     1681 aaaaacctgt tataacccct gaaataagaa gaacaggaag atagatataa attttaaaat
     1741 cttgtatata aactgcggct acaaataact gcccaatatt atgaaatatt gcaccgcaga
     1801 cacttatgga actgatactt attccatctt taaaaaactt atataaaata atcataacga
     1861 tattacttag aaggcctcct actatgctaa ataaaaatac agaaaaacct cctccataaa
     1921 ttgaactgat aatggttctt aaaacaacaa tcacaagcgc ttctttccag cctaatacca
     1981 gcaaggcaaa taaagaaata atattagcca gccctaattt cacccctgga aaaagtactg
     2041 gaatctgtcc ttcaatgata tgaataacta cagccattcc tacgagtaat gataaaaaaa
     2101 ccatcttttt tgtcttattc attataccac cattctgcca ttaatatgaa atttcatcaa
     2161 gttcctcttt ttctccttct atttttacaa ggacattgtg aggcatacaa acagctatat
     2221 ctccgggttg agataaccat cctgtatgga cgcaaaccaa atcagggcaa ttggcttcaa
     2281 taaaacggat tcttcctttt tcgactctta tgatattata atgcccttct tcacccttta
     2341 ccatccattc ctttggttcg tcaatttttt ctaaatcaac ttcgacaaga actttttgat
     2401 tttgaataat tttagcattt aatgcttttc ccttataact tattctataa actataaccg
     2461 ataaaaagct aacaaccaat aaagccaata cagtcatgag aacgattttg tcaccttttt
     2521 tcattttctt cacctttaat taagattttc cagtaactga aactgtggat tacttaattt
     2581 aaaatcttct cttaaacctg cagttacgta tatttgatta tcttccgtaa taatcacagc
     2641 ttcaacattt tccatcctct ctatcaattt taatcctttt tctaacccca taacatatac
     2701 agcagttgat aaagcatctg catcaatgga ttgatcggtc acaattgtac tgctgataat
     2761 acccgcttca gcaggataac cagtttttgg atcaataatg tgatggtatc ttttaccatc
     2821 tttaataaaa tatctttcgt aatttccgga agtcaccaca gccttatcct tgacttctat
     2881 aactgctaca tgactgcccc gttcatccag tggattttga attcctatct tccatgggct
     2941 tccgtccact ttcgttccaa tcgctactac atttccgccc aaattaacaa aagctgtctg
     3001 aattccatgt tctgttaata ttctttttac ctcatcccct gcataaccct ttgcaattgc
     3061 acccagatca attgcctgtc ccttttttct cagcataacc gactgacttt cctcatccaa
     3121 tataagatct tcataattaa ttaaatccag caggacatct atttcttctt gttttggaat
     3181 tcttgcattg tccgttccta tgccccacaa ttttacaata ggttcaatcg taaggtcaaa
     3241 agctccctgg gaaagtttac tatattcact ggctttttta atcacaaaaa aagtatccgg
     3301 gctaacataa ataaaattta ttcctgcatt gttatttatt tcaatcagtt cacttttatc
     3361 tttattgatg gtcattttat cttcaatttc tctgattcgt tgtatggatt tatcaatggc
     3421 ttcttttgct tttttgctgt catcatcata tattttaaat tcaataatgg tacccagggc
     3481 aaaaacttct tcctgtatga catttgcctt attggattga ttgttgcaag ccataagtgg
     3541 aatcattatc attataagta aaacaacaaa attcgtgact tttttcattc ttccaccaac
     3601 ttctctttgt atgatattac atattatatc tttataatgc attaaatatc aattaaaatc
     3661 gaatttattt ctaaaagact taaaactctt taaataaata taaaatttat aatttaaaat
     3721 aaattcccca agtcttaact tggggaatgt aaaatgatat gtttaatttt ttgcttcttc
     3781 taaagcttta tttgccattg tcttgaagtt attcgtagta cccgttgcac cagttactgt
     3841 atcaactgca ttcacatcct gtttttcaac caaatctttt tccaattgtg ggatagcttc
     3901 tgcgggacca agtccaacct tatctttcat atttttgtta tattcttcat cagtctcttt
     3961 agaagcacct gcctcattca cttcgtcata atcaacagac gaaattttac catcttttac
     4021 ttcaatggtg ataacaggtt tccagccatg ttcatcaaaa ctatctgcct cagccttata
     4081 actgccatcc ttgtaagata cttctgtttg tgtacctcct gttgtatctc caccatcttc
     4141 tgcttgtttt cctgtattac aagctgataa tacgctaact gccaatgcta aagataaaca
     4201 cattaatata attttcttca ttatagacct ccttttttgt atataaattt aaattgtact
     4261 agcttgttaa tccttagaca agtgacattt tactatagta atcacttctt gtcaaaatat
     4321 ataatttagc atttattata cttttaaatg atttatgagc tttttctatg ctattctaag
     4381 tattcctctc aattttgttg ccttaattat atacatttaa cgataattat gatatactca
     4441 ataaaaagag aacattttta aatttataat ttgattagga atatcttcat gattaatatg
     4501 cgtcaaaatg ttaataatat acactaagga ggcaaattgt atgaataaaa acagtaaaaa
     4561 agtattcatc cttggtgctg gatacggtgg tattcaagct gcactgacct tacaaaaaaa
     4621 gaagaaaaaa tcggataaca ttgagatttt tattatagat aaaaacccct atcatacact
     4681 gctcacagaa atccatgaag tcgcagcaaa ccgtattaat gaaaacggcg taacggttta
     4741 cttaaaagat attttccaat atacagatgt aaaggttatc caagactata ttaaagatat
     4801 tgattttgaa aatcaaacat taatatcgga aaataatagt tattcctatg actttcttat
     4861 tattggcatt ggaagtgaac ctcattatta tggtattgaa ggcttaaaag agaatggctt
     4921 cagcctttgg tcccataagg atgctgttaa aattaaaaat catattcttt ctatgtttga
     4981 caaagctgca aaagaacccg accctgaaaa aagaaaagca tatctgacct ttgcagtagt
     5041 cggcggtggt tttacaggaa cagaaatggt cggagaattg gctctatggg ttaaagactt
     5101 agcaaacatt tataaggttg atttaaaaga agtaagactc ttgcttattg aagcactccc
     5161 caaaattctc tctatattag atgactcttt aatccataaa gctgaaaaat atcttaaaaa
     5221 taaattaggt gttgaaattc ttacgaatag cccgattaca aaagtcaata atgattcctt
     5281 tgttattaat aacgaaaaga ccattaagtc tcatacactt atttgggctg gtggtgtaca
     5341 agctaataaa tgtttaattg atattgattt agaaaaagga aaaggcgaga gagtttgtgt
     5401 agatccatac acgagaacca agtataaaaa tgtctttgcc attggagact ttgcattatt
     5461 taccacagaa gataatcaaa tcctgcctgc cctggttgaa acagctcttg aaactgggaa
     5521 atatgcagcg atcaatgttc tcaacctgat ccgtaatcag gaattagaaa aagccaaacc
     5581 caaactgcat ggtattatgg tctccatggg taaaaactat gccgttgcca atattatggg
     5641 cataaaatta tcaggctacc ctgctttatt aatgaagcat atggttaata ttcactatct
     5701 ctttggcatt ggtggttttg aattaatcgg caaatactta aagaacgaat tctggggtgt
     5761 taatcatcaa agaggtttcc tgcacaggca tctggataaa gtatctccta catttttact
     5821 tgtattttta agactttacc tgggttatat gtggcttatg tcaggtcttg ataaaattaa
     5881 tagtggctgg tttcaatacc aaatgcttgc cggaagcaac gtcgatacga caacaggagc
     5941 aaccgttatg cagcttgttt caagtcatac acctggttgg tatgcatggc ttgttgatca
     6001 tttaattatt ccaaattcca tgctcttcca aaagttggtc gtgttgaccg aaataggtct
     6061 tggtttagcc tttattacag gtacctttac tttcctggca gctattgtct ccatcggcat
     6121 gaatatcaat ttcttgctgt ctaccggact gaatgattta tggttcttaa tttcttccat
     6181 tcctatgctg ggtggggctg gaagagcctt tggtgtagac cattacctga tgccatatct
     6241 tatgagacaa tggagacact tcgtcagaaa aagaagaatt aaaattaatc tatggaaata
     6301 aataaatcac cggcaagccg gtgattttgc atttataata cattaataat atgatagaat
     6361 aataaaagca aaatacaaaa agaaaaaatc atatcagata ggagcattct atgattagct
     6421 taaatataac agatgtaaaa aaaaccatgg atgaactatt aaaagggaat ctttttgatg
     6481 aatttgagct aaggactgct gaagtacata cctttgcaaa attccaaatt cagggtaatt
     6541 taaacaaagc ctttttaacc gaggatgaaa aagatttata tgagcgcaat tttgtattat
     6601 ggaaagaaat caaatcacat atatttaata tcatcaaagg aaaaaagccg ccgacctatt
     6661 taaaaatagt attttcagca gctatagata agatacctga ctttgcttct tttgatattt
     6721 ctgccctgtt tatcaattta atctattcac aaggtaaatt atcctgtacc aacggcatag
     6781 catttgaaaa cttttctttg gataaaacga ttgaacagga atgggatcaa tatgttcgtg
     6841 acttttttaa aaaagcgaat atctatgtag aatgatttaa atctctaaag ttagaaaata
     6901 atctacaata ttttcaatag aaataagccc tatgaccctg ccctgctcta ccacaggcat
     6961 acctattata ttatgctgtc gcaatcttct ggctgcctct ttaatggatt catcaggcat
     7021 tgccgttata acatctttac tcatcgccca gtctaccggg caagcttcgt agtgacctgt
     7081 tgtactcaaa aatctatata tatcggcttt tacaataaaa cctactaaca acttattttc
     7141 atctaccaca ggaagaccat tcactttttt agtctccatg atggataaag tcttttctaa
     7201 agtatcctga ggcataacca tataaaaggt atcattcata atatctctaa ccttcattta
     7261 aaatcacctc tttttattga tttcaatttt attatatcat attttctaca aaaagcctac
     7321 cttataattt gtcactcacc aggaaggagt gctgatgatt cattcttgac ttttattttc
     7381 agcgtggtat aacatatgat agaaaaaact aaagaaagga gaagttctat ggctcgtgaa
     7441 actggaaatc tatccataca tagtgagaac atttttccta tcattaaaaa atggctttat
     7501 tccgattccg atattttcat ccgggaattg gtttctaaca gctcagatgc catattaaag
     7561 ttaaagaaac tggttacttt aggggaagca actgttcctg aaaatacaga atatgaaatt
     7621 gaagtcattt tagatgaaaa gaataaaacg ataaaagttt cagacaacgg cattggcatg
     7681 actgccgatg aagttaaaaa atatattaat caaattgctt tttctggtgc cgaagacttt
     7741 ttaaaaaaat ataaggacaa aatggataaa gatcaaatca ttggacattt tggccttgga
     7801 ttttactctg ctttcatggt agcagacaag gtccagattg ataccctttc ctatcaggaa
     7861 aacgcctccc ctgtccgttg gatttgtgcc ggcggaacag aatatgaaat ggaagaatcc
     7921 gatagaaata cccgtggaac aactgttacc ctctacatag gtgataatgg aaaagaattc
     7981 ttaaatgaat ttaaattaag agagattctt gaaaaatatt gtgcttttat gcctgttccc
     8041 atttattttg aaaaagcaga acacaagaaa aagaaaaatg aagaagataa aaaagaagaa
     8101 aggaaaccca ttaacgatac taaacccttg tggttgaaaa agcccaatga ctgtaccgaa
     8161 gaagaatata aggactttta taaaaaagtc tttatggatt ttaatgaacc tctgttttgg
     8221 atccatttaa atatggacta tccttttaac cttaagggaa ttctctactt ccccaagcta
     8281 agaaatgaat ttgaaaccat agaagggaag ataaagcttt ataataatca ggtattcgtt
     8341 gcagacaata taaaagaagt tattccggag tttttacttc ttttaaaagg tgctatcgat
     8401 tgtcctgatt taccccttaa cgtatccaga agttttttgc aaaatgatgc atatgtcaaa
     8461 aaaatatcca actatattgc aaaaaaagtt gctgataaat tagtttctct ttataagtca
     8521 aagaaagagg attacattaa gtattgggac gacatccatc cctttgttaa atttggagct
     8581 atcaaagaag aaaaatttta tgataaagtt aaggacacga ttctttacaa gagtatcaat
     8641 ggaaattact taacccttaa agaatactta gaaaaaaatg aatccaatca caaaaacaaa
     8701 gtcttttatg tgaccgatga acgtcaacaa gcacaatata taaaactttt taaagataat
     8761 aatatggatg ccgttatttt aaactcaacc atagacgctc cttttatgtc tcatatagaa
     8821 atgcgtgaaa gcgacattaa attttacagg atagacgctg atttgtctga aaatctcaaa
     8881 aaagatgaag ataataaggg cgatgaaaaa gcaaaagatt ttgcgcaaaa acttgaaaaa
     8941 atctttaaag ataacttgaa taagcccgat cttaaagttc aggtagaaag ccttaaatct
     9001 gaagatattt cagcaatgat tcttctttct gaagagacca gacgcttaga agaaatgtcc
     9061 aaaatgtttg gcggtctcga aatgccaggg ctttttacca aagagcaaac ccttgtcctc
     9121 aataaaaata atcagttaat ccaaatgatt tacgataaaa gtgaagacga aaatgaaaaa
     9181 gaacttctta acatactctg taatcaaatt tatgatttgg ccttattaag tcaccagact
     9241 ttagaagctg aggccatgac tcagtttatt gaaagaacga ataaaatcat ggccatcctt
     9301 gcagaaaaga attaaaaaaa ttgcctccat aagccagact taaagtctaa cttatggggg
     9361 caattttatt cttcatagaa aacggctctt gaaatcccaa aaagatacag gcacttttct
     9421 tttactttaa gacctgtaat ttcttcaatc gccttcgtat aaatatttat ttgtactcta
     9481 tacttatcca taattttttc aatttctttt tctggattgt ccttatttaa tataaaatct
     9541 gttttataat ccactaaaac gataccatct tcttcttcaa agaaacagtc tatgatcccc
     9601 tggataaata tttcttcttc agagtttaaa cccttatata tttctcgggc atcaagagac
     9661 aagataaaag gggtctctct ttttaccaat gaagattttc taattctttc tgccagccct
     9721 gatttacaga aattcaataa tgaaggaata tatacagctt ttctttcttc ttcggttaac
     9781 aagcccttta actccatctt tacaagctgt tcttctatgg ccttatagtc ttttgcccca
     9841 aaatctaaat gtctcatcac cttatgaata atggtacctc tttctgctcc tgtaaaaacc
     9901 tgtccttctt cgatgaattt aggctgtctg ggctgtaaat cttcaaaaac agaatccata
     9961 ggataatccc tatgattttc ttcatactga cttttgattt ctgaaatcga tagtttgact
    10021 gaaatcttcg ttgccgcttc ataaggatat ttccaggata aacgcctgaa gatttcttcc
    10081 cgctcagggc tgtaagtttt actgtagtcc cagttaagga gccggttaag cacctcttcc
    10141 tttttgactg ctttgctctc atcttgaata tgaatatctc ttttagacca ttttataagc
    10201 tcccatgacg atgtatcttc ataaagactt tgtacggaag agccaaaaga attcccccag
    10261 ctcttaatct tctccccgtc tttatgtctg gctaaggcca ttccaatcca gtccagatag
    10321 gtcctggatt ttgacaagat ataaggagaa agttcttcct tttcagaagc cacttgtctt
    10381 acccactttt ttgcaccgct ttctatgctt ttaacgctgc cggtaaggat aagcttttcc
    10441 ctggctcttg ttaaggcaac ataaagaatc cgcatttctt ctgataaagt ttccagggtg
    10501 atttttcttt taatggcaac cttggcgact gtctcatatg aaagcctttt ttcataatcc
    10561 acataattgg gaccaagacc caaatcctga tgaagtaata caggattatt taaatccttc
    10621 aaattaaatt gttttcccag gcctgccaca ataaccactg gaaactctaa acccttactt
    10681 ttatggatac tcataattct tacgatattt tcattttctc ctacgatttt ggcagctccc
    10741 atgtcccctt tgccacctgt ctgcattctt tcaatgactc ttataaagtt aaataaacct
    10801 ttataactgg ttgcctcaaa attggccgcc ttatccctca gcattcttaa attggcttgt
    10861 cgctgttctc cccccggcat agcccctaaa taattataat aattactttc catatataaa
    10921 atccataata gttcatcgat ggggatatga gctgctaaac tgcgccatct ctctaagtta
    10981 tttataaaat ttttgagtct ttttgaaagt tctgtttcct gaaaatcatc ttcaatatat
    11041 cttttaagag attcataaaa atccccatac gaaaagctcg ttcttatttc taccagatca
    11101 tcacagctta cgcccacaat gggtgacctt aaaacagcaa taagaggaat atcctgtctt
    11161 ggattatcaa taatatgaag taaagccaga atggttttga cttctatcgc atcaaaataa
    11221 cccgaagata catctgcata ggcaggaatc ccttctttag ttaactcttc agtaaatgca
    11281 tgcgcccagg tctccgtggt acgcaataaa ataacaatat ctttataatc tacttttctg
    11341 tatgctttga gttttttatc aaaaatataa aaattatctt ccagaactaa ccttttgatt
    11401 cttcttgcga ctatcttagc ttctaactca atactggtca gagcttctaa ttcttcatct
    11461 atattgtctt ccttttctgt gagctctgat tctatcaaat gcaattcaat gggtccgccg
    11521 aaatttaaac cttcttcatc aggataattg gctgcataat taagagctgc tttttcatca
    11581 tattctattt ctccaaggcc aggcgtcatc aattgtttaa agagaaaatt aaccccatcc
    11641 aggatattct tgcggcttct gaagttttta aataaatcga ttctctgctc tttaccagtc
    11701 gcttctgttg aaaaagaatt atacttttct ataaataaat cgggctttgc caatctaaaa
    11761 cgataaatac tctgcttaac atcccctacc ataaagcggt taggcatacc cgcatacttt
    11821 cctgaaatca attcaaggat ggtttcctgt accaaattac tgtcctgata ttcatcaatt
    11881 aaaatttctt tataacgctt ttgcagctca atagcaaccg ctgaaggaat aatttgctcc
    11941 atgctgcttt tttcatctaa taaaatattt aagcaataat gttctatgtc attaaaatca
    12001 ataatgccct tatccttttt tactgcctga tagcggtttt caaattcatt taagacattt
    12061 tctaaagctt tcattacagg atataactta tgtaagtctt tttccatttc ttttggggat
    12121 ttaaacaata atttgtcctt aaagcccttt tggatgcctt ctcttatgct ttctctaagc
    12181 attttaactt ccgtttttat attttcatca atgtctttac tgcgttcgtt taaaggcttt
    12241 aaagaaatct gctgtatgta gttatataat tcttcaatat cgccctcaca aagactaatt
    12301 attttttcta tatggcttat gtcatcattt aaggcatcta tgaatttttt aggaccagca
    12361 ggttccatac aaagctttat cgcctgtttt gcaatttcta aaagttcttt agattcctca
    12421 agaatttgct ctttaatcat ctttatccaa atgctatttt taaagtcaat ctgttccgga
    12481 ttaaagcttt ctactttttg tctaagccag tcttttggcc agggatgact ttcaataaaa
    12541 ttataaatat ccagaagcat ttccttaagc tttgtatctt cttttttacc tccatagctt
    12601 tctaccaggg ttaaaaaacc ttcatcttct ctttgatata aattttcaaa gagttcttct
    12661 aaaacatccg tttttattaa aaggatttct ccttcatccg ctattctaaa tgagggttct
    12721 atatctaaca aatgaaagtt actttttata acatttaaac aaaaggcatg aatggtagaa
    12781 atggacgctc tatgaagaag ggctaattgc ttttgaaggt gaaaatttct gggttcttct
    12841 tcaagttttt tagataaagc ttcccctatt ctttccctca tttctgctgc tgctgcattg
    12901 gtaaaagtta caatcaggag cctgtcaata tctacaggat tatctttgtc cgtaatgata
    12961 ttaatgattc tttcaaccaa tacggctgtt ttccccgaac cagcagcggc cgcaacaagt
    13021 aagtcacagc ctcgtgtata aattgcttct tgctgttctt tcgtccagtt cataaaagcc
    13081 ctccttaaca actactgaac actcttccat aaagcagtca tttccgtgac aatatcttca
    13141 acgttcctgc ccgcgcactg aatgatttta tctgcatatt tttcatataa ggggactctt
    13201 tctttataga catctaaaag ccccttattt ttatccatag caatacctct tgtcttaata
    13261 ttgtaaagcc ttctctggat aactttataa ggcaaattca aatagaaaac aaagcctgtt
    13321 ttctttaaat gcttcatggc ttcatccctt aaaataacac tgccacccgt tgcaatcacg
    13381 gtattagaaa ccttgacttc taaaataaca tttttctcaa tatccagaaa ttcactaatc
    13441 cctttttctt cgatgatttc ttgcagtaat ttattttctt ttttttgtat taataagtct
    13501 gtatcaagaa aagacatatt taaagccttg gccaataaca cgcctatggt gcttttccct
    13561 gcacctggca tccctattaa tacaatattt tctttcattt ttaacctctt ttcctatgca
    13621 tattctaaag atttgcaaat ggctaaaaga gcctgtccac attttaacag gtcctgttga
    13681 ttttcaaaaa cagcctgggc ctgaatggga ctgccgccac ctttgccgcc aaatttactg
    13741 acttcacttt taattaaatt accacagtgt atcaagaaat ctgtattatg ggctataaat
    13801 aacttcattt cttttaatga aaggccaatg aatatgtatt tacccatctg caaaacttgt
    13861 tttgaaataa aaagaatttc atccatggac ttgttgtcat attgtccata tataaccgat
    13921 tcctctgcat tttttaataa tttctgagct tcgtaaagga caagctcttt ctttaattcc
    13981 ttaatttgat tttctttttc cttcacgtct tctaaatatt tctctacttt tttaagcaag
    14041 gtattttcat tggctgaaaa agtctcattt aatgtgctga tgacatcatg ttttttgcta
    14101 taatcttcaa aagccctcct gccgcattga aagtaaattc ttgttgcccc tttagacttt
    14161 tctgttttaa taatttttaa tatgcccact tcccctgtcc ttttaacatg agttccacag
    14221 caggggcaaa agtccacacc atctatttca actatccgga tatcctcatc tactttaggc
    14281 tgttttctaa gaggtaattt ttttgcttct tcaatatctt ttaaatatgt tttgacctca
    14341 acatttttat aaatgatttc gttaaccttg tcttcaattt cttttaccat ctttggggtt
    14401 acttctgaaa ggctgatatc aatggaagta tagtcttctc ccatatgaaa agcattacta
    14461 accccctgat aatatttata aaaagttgcc gccagcaaat gctcgcccgt atgctgctgc
    14521 atattggtaa aacgcttttc aaagtcaatt ttacattgaa cctttttttc ttttggagct
    14581 ttttccataa gatgatagat ctttccgtct tcttcaaaaa cgtaagagac atatatgtct
    14641 tctattgtcc ctgtatctga aggctgtccg cctccttcgg ggtaaaagta ggttttatcg
    14701 agcacaacaa gatatttatc atccttagga atcaactcta caatttcgct ttcccagaca
    14761 gatgtataag ggtcatcata ataacatttt ttagtcattt tccagctcta ctccttttct
    14821 attgattctt ctatcttttt ccaaatctca tctttactca atttatggag atatttataa
    14881 tgattgtctt ctaaaagggt atcaaactga catatcgatt tgtataaaca gtattgacaa
    14941 gatgaacctt tatctgactt atagggactt atttttacat taccttttat aatttcttct
    15001 ccgatttcta cggccatctt gcgaacatat tctcttaagg aatcaaactg ttccatcgta
    15061 gccgtgtttg actttttgcc aataccgctt ttggtcatct ccacagggat aatatccgaa
    15121 cttttttcta ttgtattatc cattttcctt ataatctctt catcttctaa cacaatacca
    15181 gaaagcttta attccttaag caggagtttt tgtaattctt cttctgtcat ttccccagcc
    15241 gaagagatga taggatcatc aattctaaaa taaaaggcac cagcaggaaa tatctttttg
    15301 ccatataaaa tctgcccgct ttctaaaaag gcatccaggt ataaaataag ctgcatttgc
    15361 atcccataaa aaatatctgc caaattaaag gattgactgc ctgatttata gtcaataatt
    15421 ttaacaaata ttttatcttc ttcatccagg atatccactc tgtcaattct tcctgtaaga
    15481 atcagttttt caccattaga aagctcaata acaattggag gaaggggatg attttctcca
    15541 aagcctattt cgaattccgc aggtttgaaa ttaccccttt ttacatgctc tgttaaggcc
    15601 caaactgccc ttttggtaat tctttttatc cttcgaatta aatatttgtt cttttctgta
    15661 cttaaaagaa tctcattgcc taatttaggt gctatttcat tgatcgtctc ttccaccatt
    15721 tgatccctta gaccatcgtt taaatctctc caggataagc cactgtttaa aagttttttt
    15781 gaaaaaatat ccagggcact atgaaacagg atacctaaat ctggaagact caattgataa
    15841 attttccttt ctttagcttt taaaccatac tgcacaaaat aaccaaaagg acaggcagca
    15901 aacttttcaa gtctggaaac actggtatat attttatctt tgtataattt cctgacagtt
    15961 tcttttttca gataatattc ctggttgtta tgaaataagc cccgaatggt tatattgact
    16021 ttatctttcc agtcttcctg actgacatac cagctaagca catctttcca tacatctgaa
    16081 aaatcgtctg catctaaaag ttctctcagt gcctgtccta aatatgagaa tgtagctcca
    16141 ggagaagaaa taaaatccga ttcttcagac ataatcagat cactttcctg ggaaagtttt
    16201 gggaaaatct tttttatcct cgaaataagt accgatggtc ttaaggcttt tccctcaaca
    16261 tctcctaaag aaaaacttaa atgcaaatat tcttctggtt ttgtaagtcc cgaataaata
    16321 agaaactgtt cttcaaaagc ctttcttctg ctatccggag ccagctcaac gcccaattct
    16381 tccatagttc ttctttcttc atctgaaaac aatcttaatt cttctttatt ggcaggaata
    16441 atcccatcat taataccaat cacaaataag gccttgatac gtggaagtct cgacctttcc
    16501 aaatccgcaa ctacaatttg atcaagagcc ggaggaataa ggcccatttt acattgttca
    16561 aaacctgcat ccaggactct tggatattgt tttaaagaga ccggttcttc tcctaaaatt
    16621 tctaccattt tatcaaagag ttcaacaaca aaatcccaaa tttgaagatt ctcattgact
    16681 aaagataatt tgttatcttc cttaaaatga ctaatgtact ctgccagtct ttcccttacc
    16741 cctaagtcct caagaaattc ataaagagcc tttgttattg aggcaaaggt atgatttttc
    16801 ttatttttta acttttgact taattttata agtggttcaa taaatatgtt tcttatttga
    16861 ttgattcttt ctaaaaggct ttcttcatat ttcttttgaa gttcatcttc aaagtcttct
    16921 tttattattt tccagtcttc ttttgtccac ttactgattc ctttaatgcc ataagctaaa
    16981 acataatttt ctaataaatc cacatcttcc atagcaatgc ctgtaaagcc tgtttttaag
    17041 tatctgaata cactttcata actccagttg ctatttaaga tttctaaagc cgcacgtatc
    17101 aattcaatta aaggatgaga aaggatatct tttttctcat caataaaaaa tggaatatta
    17161 tattctttaa aaatagcttc tatcagtttt tgatactgag atatcttccc tgtcacgact
    17221 gcaatttccc ggtaacgata gcccttgtcc ctgacaaatt ctaaaattaa tctggctgta
    17281 tgctccaatt cactataagg attagaggca acaaagagtt ttatatattc tgttttattt
    17341 tcataaacct ggactggata attaaaatat tctttttcta aatgatacaa ttctttatta
    17401 ttaatacctg gctctttttc attatatagc tttacaggtt cctctaaagc aatattataa
    17461 aaggccgtta atttattcag tttatccatc gtccttctgc tttcataaaa aggatcggtt
    17521 tcatcaaaag gctgatacaa atctcctctg gggtctaaag tcagggaaat gctaacctct
    17581 tttgcttttt taaataagcc ggtcaatatc ctgtactgct ggggcgtaaa tccataaaaa
    17641 ccatcaatcc agatataggc attttccaga aaatttgagt ccccaatttt ttgtgctaat
    17701 aaatcaaggg tttcctgaga agtaatatac ttttctttaa tatagttatt aaaattctga
    17761 tagatcaaaa ataaatcgtt tattttagcc tccaaaatgg gcctgtcttt aataaactcc
    17821 tttttttcca gtaaatctgt gggtcttaca tcatactgct ggaattcaca aatcatatcc
    17881 cctaaattct gcataaaacc tggctgtttt acagatttat gataaatctt aagttcttca
    17941 cttatatcat caatgacctt gcttaaaacc atattccttc ccaattcgcc caggaacttt
    18001 tccctggggg gacccagttc ttcaaaaatc ctgaaagcca atctttgaaa actaagaatc
    18061 tctactgcca taattccttt ttgattaagg aaatttatta aatctttctg ggtttgaaaa
    18121 gtaaattgtt caggtacaat taaaatccgt gcaatatctt ccttttctct taatttatcc
    18181 ttgatatcat taaagatata atgggtctta cctgagcctg aacgacctaa aatatatcga
    18241 acactcataa tatctctttc ccaccttttg gtgatattat tcttctatcg tatacacata
    18301 tagttcctta tctcttaaac tatctttatt taaataggct tccatttctt ttggaattga
    18361 aatacggtct ggatttccat cagaaatata tctgtattca ggatgcttgt cataataatc
    18421 aaaaaaccac cacaaatccc attcctcgcc ttcagaacct cttactctgg gcacattaaa
    18481 aggctcaaat ttaacatgat tgactgggac aaaaggacca ctgggacctt ctcttaaagc
    18541 aatcttataa tgatactcct gaccattata gacacctttt tcaataagat gtcttaactc
    18601 ttcctttggt ctttcaccaa atggataggc taaagaatcc acgacataac caggcaatgc
    18661 ttcttttatc atctgatcca gcttcccaac ttgttcttgt atctcatttg ctgataactg
    18721 agataaatgg gcatgggaat aagtatggtt atatacttca taaccatggt caattaaata
    18781 tttaaatcgt tctttagttg ttcctgcacc tgcaaaagga tcgttgtctc cattaatata
    18841 aaatactgca tgtcttccaa agtcaggatg ttcttcacag aatttattga taatgtcaac
    18901 agcacaatca ggctttgcct taagcacgcc actttcttca atcagtgaaa aactgctgct
    18961 tatcccatca tcaaaagtaa taattacagg cgtgcagccc gcttcaacag aaatattatt
    19021 atcaataaaa tccctcatgg atatagcccg gtaaccatgt tcatataaat actctaaatc
    19081 tttcttaaag ccttctatag ttctttgata aggagaagga ggattctctt ctactaatcc
    19141 atgatacata ataatcatta tatgtcctaa ttcatttggc ctaattttat tataatcaat
    19201 ttctacgatt tcattttttt cttcattttc ttttgcttgt tcttggtctt cttctgtatt
    19261 ttcttctttt tcatcatctt ctgctaagtt ttcttctttc tttccttcag taatcatttc
    19321 ttcccgattt gtcttgtcgt tattggaatg actagtatta ttacaagcag gcgagattaa
    19381 tgaaataagg caaatcaata aaagaactaa cttcaaattt ttaagcattt tatataaatc
    19441 cctccagtcg ttttatgtca ttattattat actattattt atagctcata ccaagaaata
    19501 aataatttta ctaattattt tacatatttc gaactaattc tataaatttt gtcttttctt
    19561 tctttaatca atggtttata attaaataaa gataacgaca aaaattaagg ggggccatgc
    19621 tatgggattg ttgaggaaaa agaaacaaac taataatcaa actcaaaatc aacaggtttt
    19681 ggatgaatac gctgcaaagg tgaatgaagc acttgtccct gttaaattct ctgccaaaga
    19741 attgcaggac attcttgcca aaatatatac acccacacaa accatttcat cccttttagt
    19801 tcaaccagaa actgtgcaaa cagataactt acaggagctt tctattaaat atcaaaactt
    19861 ggaaagtgaa attaagcaaa tctccagaaa cattgattcc atgaaggaaa ttgctgaaaa
    19921 tgcagtaaat acagctaaag atggacaagt attccttaga gaatccattg ataaaattaa
    19981 taaacttaga aatacgatag aaagaaccaa tcaaacgatt agttctctta ataaaagttc
    20041 cagtgaaatt ggtaacattt cacaaatcat taccagtatc tccactcaaa caaacctcct
    20101 tgcattaaac gcttctattg aagctgccag ggctggagaa cacggtaaag gttttgctgt
    20161 tgttgcagaa gaagtaagga aattggcaga gcagtcaacc aaatctgcag aagatattac
    20221 taatttgatt tcaggtctgc aacaacaaac cactgaaatt tccgaaatta tgtccactag
    20281 tgccagtgaa gttactgaaa ccgtttcagc cgttaataaa gctggcaatg ctttcgagat
    20341 tatcagtcaa tttgttgaaa ccatcctcac acaatctaat gcaatcaata atactttaga
    20401 attaatcatc agcagcatga aatcctttgc aactgcagtt gaaaattcat cacatacaac
    20461 agcaaatgat attttattag aagatataaa acgaaacaac caggctcaaa ttcaagcatt
    20521 gggtgaattt aaacaggctt acgataactt aattaacaag atttctaccc ttcaaatctt
    20581 agcacaagcc tataatgata attaaaataa gtagcgtatt aatacgctac ttattttttg
    20641 agagttacaa tcgttttaaa taaatctcca tctaactcta tttccagttt cccgccatga
    20701 ttgtctacaa tagactgtgc aatggcaagc cccaggccag aaccttcggt atttcttgat
    20761 ttatcacctc gtttaaacct ttctaaaagt tcttctatat tttcttccaa ttcgtacttt
    20821 gaaatattct taaagctaat catcacataa ttatctgttt cttttattga aatatatact
    20881 ctggtgtttt ctaaggaata tttcaaaata ttgctgataa tgttatcaaa gaccctccac
    20941 attttcctgc catcgaccat cgcataaata tgcggactgg aagaacttac tttaaagttt
    21001 aaggaagaat tattaatctt ttcatcatat tcccctaacg cctgattcat caactgtaca
    21061 atgtccactt tttctatatt taattcaata ttcccactag ccattttaga gacttcaaac
    21121 aaatcatcaa taaggacttt taatcttttt gattttttct caataatgtc tatatattct
    21181 tttcttttct cttccgaaag gtcttctttt tttaaaagct ccgaataatt cataatggag
    21241 gtcaaaggtg ttctaagatc atgactgaca ttggttatca attctgtttt taatcgttca
    21301 ctttttaatt gtgccttatg ggaataatct attccttgcc gcatacgatt taagttgcct
    21361 gctaatcttg ataaaacatc tttacctttt acaacaagat ctgcattggc ccttcccaag
    21421 atgatatcat ctgaatatct cactatccgg ttaaaatagg ccagcttact gaaaatatat
    21481 agcaaaatgg ctaaagtcgg tattagtccg acaggaacta aaaccagcaa gtcaactggt
    21541 tctattagtg gtaaaaccaa acctacaatg atccctaaaa aagagacgag ccctaaaatg
    21601 aagaatattt taaaaacaga acttttgaca ataaaactat ccgcaatatt attcttcgct
    21661 cttataacaa aacttttact ccattctgtc tttatttgtt gtgaggagct aaatgcctga
    21721 aaaaggaata gcatttgaat aatgaatgcg attaaaaata cagtataaag taaaagatcc
    21781 acactgcctc cataataaaa cctgtaacct gcctgataaa atacaacagg ctgacagccg
    21841 aaaagaatta ataaattaac cagaaaaata aggatcttaa tatcaatagg aaaaacataa
    21901 aactttttaa gcggctccac caattgaatc aattcttttc cgtatttctt aattatgatt
    21961 aatcctaaaa ataaactaat caccaaaaca actgcaccta tcccaaaaaa cgtaaaaaat
    22021 tgcattctcg tatcttcgta gtcatacata ttttcttcta ttatagtatg ggctccatcc
    22081 attttaggaa cagcaataaa gcccgttaat ccattagtaa taaagaaacg attaacagaa
    22141 ataaatggat cgtttccttt ggtatgataa ggaaagctct taataaaata gcttgatgct
    22201 tttactgttt caaaactttc attttcctct aaattcgtat aaacttttcc tgttggttct
    22261 tttatatagt atttaaaatt cttttcagca gattcaaaaa gagttttctc tggttccatc
    22321 tcatttttaa catatgattt agttagatta tcatccaagt cttccgtata aatttctgtg
    22381 tattcttcat atgtgagctg agtaattgaa aataaattga taaatttttc tacttccctg
    22441 ctaaattcat cggtatcaaa atagctcttc ttcacacggt ctgacagatt actctttaga
    22501 agtaaagtat tggtaagccc aaaagaaaat aatagtaaaa taacaaatgc aaatatactt
    22561 aactttatat tacttttcaa ttttatatcc aattccccac accaccttta aatatcttgg
    22621 ttcactagga ttgatttcaa ttttttctct tatttttctt atatgtacgg ctacagtatt
    22681 ttccgaatta taactgggtt cattccaaac tctttcataa atttcatcaa tggaaaaaac
    22741 ccttggagca ttcaacataa gcagttctaa aattttatat tctgtagatg tcagcctgac
    22801 tttttctcca tttgcaatca cttctctggt ctcctggttt aaaataagac cattaatatc
    22861 aatgactttc tttaaacctt cataaacccc taatttcata taccttcgaa gctgagcctt
    22921 aaccctggca ataagttcaa cgggattaaa aggttttgta acataatcat cggcacccac
    22981 ttgtaagcct aatattttat ctgtgtcttc cgttttagca gataacataa taatagggat
    23041 gtttttttct tcgcggattt taaaggttgt actgatgcca tcctgtctgg gcatcataat
    23101 atccataagg attaaatgaa tttcttcttt atttaatata tccagagctt caataccatc
    23161 ttttgcatga aatacggtta tcccttcatt tcttaagtaa atttctattg catctcttat
    23221 ttctttctca tcatcaacaa ccaaaacttt ataattttcc ataggccata caacctctct
    23281 tattaaagat tataaataat tatatcacac cccctttaat ttattaattc atggcttatc
    23341 acaatttcat tgtaataatg aattcttaat aaaccttaaa tataattctt aattttttct
    23401 taatacaaaa aactcctgcc aaggatttga caggagtttt ttcttatatt atttgcatta
    23461 tctttgacga agtttttcat gtaagcgaat aagtattgtc atgagctctg ctctggttaa
    23521 atctttcttg ggtgccatat ggcagctgtc tacaccagat atcaaaccta cccttacgca
    23581 ataattgatt gcttctttat atggtgaatt ttctatatct gcaaaagcaa tttcatctct
    23641 tacaggctca agtttgccat ccgtaatttc ataaatgatt tgacacaata attctcttga
    23701 aatatctcca tcgggcccaa ggttacttat cgtttctaaa gtctcctcac taagtttaga
    23761 agcaatggaa gccatacttg aaaatgccca atgttcttta ttggtaatat atttttctac
    23821 cgtcgttctt ggtaatttca tttcagtaac ttgatttaag acgaataacc tatctaaaaa
    23881 tgtaaaggta tctgctactt taagtggtgc ggcaggagca aatctgccat ctggcaatcc
    23941 tgctacaagt cccatttgtg aagccctctt aataggctct cttgcccaat gtttctccgt
    24001 cacatctgta aactgaggtt cgtcaacggg tgtcagaact acctgcttac ttaacttact
    24061 ttcatctatt ccaaacagcg ttcttagttc aggcgtatta acaatgattt gaacctgttc
    24121 catgctaaat atcgttttcg taaattctcc aagaacattt tctgatacaa tgttttctcc
    24181 aagactggta tagcaaagag cgtcttctat ttcttttgct attgttttct ttttatcttt
    24241 tgttagttta ttaattattt cttttatttc ttcaactgct tcaactgcac tttttatagt
    24301 tctgccacta tcttgcctat cttctttagg ttgaattatt tcattgtctt cttgtggcaa
    24361 aattttgtag ttaagaacta atacatcgct ctttgaataa tttcttatgg ctattgcttt
    24421 aatcgttaca tccttatcaa tatatatcgc atttctataa acattgctgc ctgaatttgg
    24481 ctctgaacca tctgttgtat aataaatggt attcaagcgg ttatctgttt ccagataaag
    24541 agattgcgga cctacatatg ttcctgaagg aaggctggcc acaggcatgc ttaatacagg
    24601 cttatcactg acatattcat ccacagaaac aatacattct gcagtaaaca cattgtcatt
    24661 tatatccgtc acttcaacgc tgatcttcgt actacctttt tttctgctat gagttcatta
    24721 ttaatcacgc ttataatcgc aggatcttcc acataataaa caatgttttt aatattggcc
    24781 tcttccggat aaacccttgg tattaatctt ctgacattac caaccttcat ctctattctg
    24841 ttatcatcta aagtcatact ttgtatgctt actgctttct catcatgagc ttttactgta
    24901 atattacaag ttttcttact tttcggattt ctgatatcat atacactaac tgtatacata
    24961 cctgtccttt tcgcaataac ctgacataca aatccatttg ggataatgat taacttatct
    25021 tcttcatcac tctccagttc ccattttatg tccgaagtag ctgcatattc tgttgtttca
    25081 atctccaaaa atattgtttc attttcatat agagataatg cagagggtaa attcttaatc
    25141 tcgccaattt ggcttaaatc tatttcagga gaaataatct cgttcgtatt gtaagaatta
    25201 tattcatcta taatagaaaa actttcaatt ttaaatttca cttttttgcc ttccaatgga
    25261 ttttttaaaa atatatccat tctgttatta ttcacaaaaa cataatcttc cagcgtcaaa
    25321 tcttgatatg acccatcatc gacagaatac tgtacatatt cttcaaaagg aattgattgc
    25381 agcggtatat caattttcct gtcgaaatcc aaatgaaatt ccttattgtt gctctttgca
    25441 ccgcaggaaa ttataataaa ttcacttccc aaatctgctg catatactat taattggcta
    25501 aaagtcatta aaaacattaa tatacttaaa caaatactta cagcccgtct tttataattc
    25561 atagtctacc tcccatacta tacttctctg cgatcaactg cgagtgtcag gattttggat
    25621 tgagcttttc tactccagga actttatatc ttttcgactg gtccgttttc aaattcattt
    25681 ccgtacatta ttcgatatct gatagatata ccagctccag aagtttgttc ataaacatta
    25741 cttgaccttg aaagtgttga acttgatgaa gaataccagc tccagaagtt tgttcataaa
    25801 cccatgcacc aatgatttta ttaaattgta tatatgtcgc ctttcttgta agtaaattct
    25861 tttttccagc catataaatt attcattgct ttccatctat agtcgcaggc taagtaattt
    25921 gctttatcaa aaatattctg ttattgatcc gactattttc ctgttacttc aattttaata
    25981 tccgcctgat tcataccatc tgttgcacgt atgatgactg ttttatcatc agtagtattt
    26041 ttaattaagt ttggaatcgg tattctcatg taattgtgag aaataacctt aggtgtaatc
    26101 tcttcttctt tatttccatc gattatcttt actcttgttg catcagaaaa tccatttccg
    26161 acaatattga tatatgaatt accatttatg taagagactt taattttctg aggtgtaaca
    26221 cgaataacat taaacggatt ttcaatatta agcttttctt taggtgtgat ttccagcaaa
    26281 ttaccattca catcataaga cattaaatat aaatcatatg ttccagcagc aaaatttctt
    26341 gaatcaataa aatatagggt taattgtaaa attaaatgcg acagtaaaaa agttgaacca
    26401 tagcgcgaaa cc
//
LOCUS       DAOSUD010000025        26277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1218_length_26277_cov_22.284189, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000025 DAOSUD010000000
VERSION     DAOSUD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 26277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26277
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_26277_cov_22.284189"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="rlmD"
                     /locus_tag="PLG49_04870"
     CDS             complement(<1..1337)
                     /gene="rlmD"
                     /locus_tag="PLG49_04870"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOQ16687.1"
                     /translation="MDSLAIKKNTNFLMTIEDLSSKGEGIGRINNFTVFVEGALPEDE
                     VEAKIVEVKKNFAIGKLIRIIKASPYRVKAPCPYTKRCGGCQIQHLDYQAQLEYKRKR
                     VTDSLERIGKLSNIQVHPTLGMENPFHYRNKAQFPVGLKKGQVQIGFYAPKSHDIIDI
                     QSCKIQDTVCDQILLIIRKFIEDNKIPVYDEEKHKGLIRHILIRTAFYTNEIMVCIII
                     NGKKLPHQEKLINELIRIPHMTSIVLNHNTKKTNVILGEEITTLWGQDYISDYIGDIK
                     FEISPLSFFQVNPIQTKVLYEKVLEYAGLTGAETVWDAYCGIGTISLFLAQKAKKVYG
                     VEIVEAAIEDARRNAKINKVDNVEFFVGKAEEIIPQFYREKGIKADVIVVDPPRKGCD
                     ERLLETMVEMAPKRIVYVSCDPGTLARDLKILTEKGYKVEEVQPVDMFGHTMHV"
     gene            complement(1354..1521)
                     /locus_tag="PLG49_04875"
     CDS             complement(1354..1521)
                     /locus_tag="PLG49_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16688.1"
                     /translation="MNKKEDKNRRLINQNNNTSESIPEKKNIIEEALKESSDEVLAKA
                     IRELLNQDKLH"
     gene            complement(1526..2098)
                     /gene="cas4"
                     /locus_tag="PLG49_04880"
     CDS             complement(1526..2098)
                     /gene="cas4"
                     /locus_tag="PLG49_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOQ16689.1"
                     /translation="MFYDYIYILPGIFLFLVISLFFIRRPDIVVKKPKLGIRAARLIY
                     TDQHESKKDPNVEYGKVLYSPKYDLRGKPDYIFEKILSKNLIPVELKSATIKEDNIQP
                     YEGDLMQLAAYFLIIEDVYKKRPKEGRLIYNNYMFIIKNTWFLRRKVKNLLADMRDML
                     KNGEQNVNPSYVKCRYCICKGTVCEFYGED"
     gene            complement(2088..2405)
                     /locus_tag="PLG49_04885"
     CDS             complement(2088..2405)
                     /locus_tag="PLG49_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16690.1"
                     /translation="MMRKDHIENKSNTISAGEINKFVYCPYQWYYQRLYGSKKLRELA
                     KIRNEYYGYEDSDLSHFNKGTGFHKNYHFAYKIKQFLLKIFWITLSILFFIHIYQVIK
                     HVL"
     gene            complement(2402..3343)
                     /locus_tag="PLG49_04890"
     CDS             complement(2402..3343)
                     /locus_tag="PLG49_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ16691.1"
                     /translation="MRYLKDIRDGEQIIGHYLCKQKQNLKSRNGKPYISLKLQDKTGF
                     VDGKIWDINCDIDEFEENSFVKIDGVVSVYQGEYQINIRRLRLSMEGEYDPMDYIPCT
                     DQDIEGMLEKLKIYIDKVENIYLKKLLQSFFLSDAEFFNKFIHHSAAKSVHHSYMGGL
                     LEHTLTVVSIAEFLASKYKQVNKDLVIAGAMLHDVGKIKELSDFPSNDYTDSGQLLGH
                     IVMGAEMVGEKIKKISDFPESLELLIKHCILAHHGEFEFGSPKRPKIIEAMIIHCADN
                     SDAKIKLFEESIQADSFQGEWVGYNRMLGRNIRKSDF"
     gene            complement(3545..4981)
                     /locus_tag="PLG49_04895"
     CDS             complement(3545..4981)
                     /locus_tag="PLG49_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ16692.1"
                     /translation="MDEDRVYNEIIKEDGLSQEMNPEVSEHTDAELNQEVTKEDRKAE
                     PEITKEAISHAPGIENSVPYYSQTIKPVKKHKLNKMKAAAIVLLVAFGGLCVGLGIGL
                     AGPVNRLFVQPALESVSERFFNADKKTEENQALFSFDNQKAEEMNAENMTRSNLPKYE
                     SIIPEIAKKIGPSVVSIRNTYVVTDWWNQERLQEGIGSGIVFHVNDKEVLIVTNYHVV
                     ANNNKLTVTFLGNYSVPATVVGLDSPSDLAVIKVLQEDLPNEIRGKVKAAPFGNSDEL
                     EVGELAVAIGNPLGDAYDNTVTAGVISALNRKIQIADKELTLIQTDAAINPGNSGGAL
                     VGSRGTVIGINSIKLVDTSVEGMGFAIPINVAKPIIEELVNKGSVSRPYLGIMGQDIN
                     EDVSELYEIPIGVLVRQVIEGSGADAAGIQAGDIIIEFDGEKITSMKQLTKIIESHKV
                     GDKIEVKLIRDGSYKKTVTVTLQQKPSE"
     gene            complement(5100..6509)
                     /locus_tag="PLG49_04900"
     CDS             complement(5100..6509)
                     /locus_tag="PLG49_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ16693.1"
                     /translation="MYKSLFSRLFISFTVIILMSFILLGVLLSQVFESYFIHQKKNIM
                     LEQGEKITMQYAKAYYTGVIDLEQLSFEMQVLDKYLDAMIWIVDADGRIYEVSRKENE
                     AWLGQQLTMEQINEVFEGKILTVKGRFGGYFKEPVLTVGYPIILGDKVYGALFMHTPI
                     PEIQKTVGELFRITVLCLGISILISFIFIYYLSKRITNPLKEMNEAAKVIAGGDFDKK
                     LSIKGKDEVAQLASSFNYMAQELNHLEELRKAFIANISHDLRSPLTSIQGFVQAILDG
                     TIPPQKQSNYLKIVLDETRRLTKMTNDIMDLTKMESGQIELNKDKFEFNDLVRRVLAQ
                     FEQRILEKKVKLYLILAEEESLVFADKEQIQRVLYNLIDNAVKFVKEEGSIWVETTLS
                     GKKLLVSVKNSGEKIPEEDLPYIWDRFHKTDKSRGKDKTGMGLGLSIVKQIIKYHGET
                     IKVENVEDGVVFTFTISIY"
     gene            complement(6639..8066)
                     /locus_tag="PLG49_04905"
     CDS             complement(6639..8066)
                     /locus_tag="PLG49_04905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOQ16694.1"
                     /translation="MDKVKDKCIFILILFFILLLRIPYISNSPYEQGENWRQSDTESI
                     ARNFVEERFNIFYPQFNYDGPLPNYVQLEFQITTFIIAILYKLFGYHYIFARVVPISF
                     FMGSCIFLYLIGRFFYSKAQSYMILILYALIPINIYFSRAIMPESAALFFYLGAFYFY
                     LKWIYKEEKKYIYSGAFFTALAISQKIPTVFIGIPMFVMAIEKYKEKIYKIKELYIFA
                     VISLGSPFLYFKWMDYIAEFKFVNKIAEKHIFKRLFTDIFTKEAIDFFKTKLPQEFTL
                     EVLLLFLTGLLMLKLKKELPIVFWLLAMLAELVFIVAVIRLNYYLIFIGPLMAIIASK
                     ALYFIWEHKYGGVFVTAIIFSIAFNSFLRVRPLYQEKSNLLKQANMVKKYTDAEDLIV
                     VGTPDPALINASERKGWRVNIDYYDHIPKDPIEEIQYYIENGAKYFVPMKGWIHNDTG
                     EYRNYLEENFKKIVDEEGYFIYILQ"
     gene            complement(8059..8421)
                     /locus_tag="PLG49_04910"
     CDS             complement(8059..8421)
                     /locus_tag="PLG49_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ16695.1"
                     /translation="MNDVSVALLSVVLGALGQVILKKGAVKSGDLSLTLFSIHKEIIN
                     LMKIPEIIIGLILFGISFLLWIKVLSKNELSLSYPLVSLNYIIILFISVLIFDEPLTF
                     KKILGTLLIVIGVGVVYG"
     gene            complement(8458..9207)
                     /locus_tag="PLG49_04915"
     CDS             complement(8458..9207)
                     /locus_tag="PLG49_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ16696.1"
                     /translation="MADKIFIGLPAFNEEKSLPELLSKIIDFSNNIDEELEVIVVDDG
                     STDRTKKIIEAFSDSYHFIKGLYHEKNLGLGAAINTIFHAVIEKACDNDFLVTMDADN
                     THNPYQIINMIQRIKEENLDLIIASRFVRGGEERGVPLIRRLYSRGAKYFFKLFFNIR
                     NINDYSSGFRVYKVGYLRRSMQLYNNQLITTNGFDCMAEILAKFSKIGIKAAEYPLKL
                     QYDLKASASKMNVFETILGYFDLLRRVKKPH"
     gene            complement(9380..9796)
                     /locus_tag="PLG49_04920"
     CDS             complement(9380..9796)
                     /locus_tag="PLG49_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16697.1"
                     /translation="MIERFIIYGLAGWCMEIIWTGIGSFLDGDIYLNARTYIWMFPIY
                     GLAILFEPIHDRIRAWPVIIRGGVYTLLIFIIEYLAGWTLQNIIGVCPWDYGDSTFSI
                     DGFIRLDYAPAWFAAGLIFERIHDLLNSIEIRRRKV"
     gene            complement(9867..10340)
                     /locus_tag="PLG49_04925"
     CDS             complement(9867..10340)
                     /locus_tag="PLG49_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16698.1"
                     /translation="MKIIRLEDKNNPVFERICEWNYNWWGIRDGRSYEEVRCNLEHSL
                     NKDRLPQTYVALIGEEAAGMYQLAMFDDLESRPDIYPWLINVYVDKKFRGKNVCRELM
                     KTVKENARKANLTELYLHTRHIGLYEKFGWVFIEEVKTFKKDSPYERLYRLEINE"
     gene            complement(10469..11110)
                     /gene="hisIE"
                     /locus_tag="PLG49_04930"
     CDS             complement(10469..11110)
                     /gene="hisIE"
                     /locus_tag="PLG49_04930"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOQ16699.1"
                     /translation="MDLMNNIKFDSQGLIPVITQDVRTKEVLMMAYMNWEALQKTFET
                     GKVHYFSRSRQALWLKGETSGNYQKVKEISFDCDMDAILILVEQVGNACHTGNYSCFY
                     RSLKGEDLVDKEKCKCGNETFQILQEVYKVIEDRRKNPKEGSYTNYLFDKGIDKMLKK
                     VGEETAEVIIASKNNSKEEIVYEISDLLYHLMVVMVQTDIQWEDICNELKKRR"
     gene            complement(11158..11919)
                     /gene="hisF"
                     /locus_tag="PLG49_04935"
     CDS             complement(11158..11919)
                     /gene="hisF"
                     /locus_tag="PLG49_04935"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOQ16700.1"
                     /translation="MNYKRIIPCLDVNHGRVVKGVNFVNLVDAGDPAEIAAIYNEAMA
                     DELVFLDITATTEGRDIMIDVVKKTAERISIPLTVGGGIRTVDDIRKILAAGANKVSI
                     GSAAFKSPELISQAAEEFGKQAIVIAIDAKKKENEEGWEVYINGGQQSTGVDVVSWAL
                     EVEKRGAGEILLTSIDFDGTKAGYDIEMTKAVADAVSIPVIASGGAGKKEDFYEALTR
                     GGADGALAASLFHFREIEIKDLKHYLKEKNVPVRI"
     gene            complement(11920..12645)
                     /gene="hisA"
                     /locus_tag="PLG49_04940"
     CDS             complement(11920..12645)
                     /gene="hisA"
                     /locus_tag="PLG49_04940"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOQ16701.1"
                     /translation="MRIYPAIDIKDGKCVRLSQGRFDKVTVYNENPVEVAKAWKNAGA
                     SYIHLVDLDGALLGNQANKEVIREIVKAVNIPIQTGGGIRNLEAIQERLSLGVARVIF
                     GTAAVKNPELVKEAIDLFGADKIVVGIDAKDGKVAVEGWEELSSVSAVDLALQMKKMG
                     VRTIVYTDISKDGMLSGLNIQATKELIDATGMDIIASGGVASLTDLEKVSKINAEGVI
                     IGKALYQGSIDLKEAISLYEGGC"
     gene            complement(12676..13269)
                     /gene="hisB"
                     /locus_tag="PLG49_04945"
     CDS             complement(12676..13269)
                     /gene="hisB"
                     /locus_tag="PLG49_04945"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOQ16702.1"
                     /translation="MERKAQVHRKTSETDIKAAINLNGTGKYSIKTGIGFFDHMLSQL
                     SKHAFIDLDIAVKGDIDVDCHHSIEDTGIVLGQVFAKALGDKKGIKRYGSVILPMEEA
                     LVLCALDLSGRPYLVFDAQFTVEKVGDMDTEMVEEFFRALCIHGGINLHIKVLSGRNN
                     HHIIEAIFKAFAKALNEAIQTDPRIEGVLSTKGVLEA"
     gene            complement(13293..14594)
                     /gene="hisD"
                     /locus_tag="PLG49_04950"
     CDS             complement(13293..14594)
                     /gene="hisD"
                     /locus_tag="PLG49_04950"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOQ16703.1"
                     /translation="MIRIIDNQDYKANELIKELLKRSQLDYKDIQKTVEEIVKDVQIR
                     KDAALLEYTQKFDRVCLTSQSLKVSEEEMNLAYKKLNSELVETLEKAADRIRQFHEKQ
                     KRNSWISPDEDGNILGQIVRPMESAGVYVPGGKAAYPSSVLMNVIPAKVAGVKRIVMV
                     TPPQKDGTINPAILVAAKIAGVTEIYKIGGAQAIAALAFGTESIPKVDKIVGPGNIYV
                     TLAKRIVFGYVSIDSIAGPSEILVVADETANPVFVAADLLSQAEHDEMASSVLITTSR
                     NLADKVKEELVRQTELLSRKAIIEKSLKDYGAIIIVNNIEEAIEITNQIAPEHLELCT
                     KESMTLLPKIKNAGAIFLGHYSPEPLGDYMAGPNHVLPTSGTARFFSPLNVDDFTKKS
                     SVIYFSEDSLKELSDDIIRFAEAEEFTAHANSVRVRFQNNN"
     gene            complement(14605..15246)
                     /gene="hisG"
                     /locus_tag="PLG49_04955"
     CDS             complement(14605..15246)
                     /gene="hisG"
                     /locus_tag="PLG49_04955"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOQ16704.1"
                     /translation="MRYLTFALAKGRLADKTMELLESIGFSCDEMKEKSRKLIFTNED
                     LKLKFFLAKASDVPTYVEYGAADIGVVGKDTLLEEKRNLYEVLNLGIGNCRMVVAGPE
                     EMKETLKFGQNFRVATKYPNIAKEYFHFNKKQNVEIIKLNGSVELAPIVGLSEVIVDI
                     VETGSTLRENGLVVLEEICPISARMVVNRVSMKIEYERITNLIEKMKKIIDNE"
     gene            complement(15243..16517)
                     /gene="hisZ"
                     /locus_tag="PLG49_04960"
     CDS             complement(15243..16517)
                     /gene="hisZ"
                     /locus_tag="PLG49_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOQ16705.1"
                     /translation="MNYKLHTPEGVRDLLPEECAKKKEIEKRIECVFNRYGYYNVETP
                     TFEYYDVFLERKEDLQQKHIYKFADREGDILALRPDMTPPIARIGATVFKEERKPVRL
                     CYLGNAFRFYESLQGKNREFSQAGVELLGINSDEADGEVIAVAIHSLLAAGLNDFQID
                     LGQVEFFKGILQEAGLSEDEGEELRQLIENKDFIGVEEFIEQFQLNPNLKELFLDLPK
                     LCGPIDIIEKTKVRTENSRARRALEKLENVYSILRDYGIEKYVSFDLGMVNRINYYTG
                     IIFRGYTFGTGSSILDGGRYDRLLGEFGHDCPAVGFGIMTDQLINAINRQKIKIPVWK
                     VDTLLVYNKEGRKQALMTADTLRREGMYIENSLLDGDIEAQKEYARSKNIDGILYFID
                     EQKIELIDLKNNQSVKTTVDQLLERSEGREEN"
     gene            complement(16724..18007)
                     /locus_tag="PLG49_04965"
     CDS             complement(16724..18007)
                     /locus_tag="PLG49_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16706.1"
                     /translation="MKRLIIHMIFFCFILTACQMSKSNIQQATYSQEEELPLSEDLFE
                     KSDYKLFILKNKSLSLYEPVNQCYIGAYILSNADLNFDIEIFEDKTQKSHGIYLRNLK
                     LGKAFPIDWVLECTAKMKTPLVILHPPSIHYPYQEYLLEETAKAFGEYYIPMFVEWYP
                     DPQQYGNAKEYKDFFMKAREIFKEYAPNVAFVWGVNHTNVKDSMLYYPGDDAVDWVAV
                     HVYDMIEDKNLAELDSSNEKIFQDIEFFYFLFQDKKPIMISQLAISHYSKHNHAYYVD
                     EAAQKIKDFYTRVKSYYPAIKAVNYMDFDNIEAAPEGEGYDNFKITAHRNLSEAYALA
                     VKTDYFTDSLDTQHIGQKENYWFVSYYPLYKKGEEFYISEKVIRYEWNTQIFNLKKEE
                     GVIIDGSLYYSLNNLVKDNGLNIIINDREKIIKVQ"
     gene            complement(18057..18605)
                     /locus_tag="PLG49_04970"
     CDS             complement(18057..18605)
                     /locus_tag="PLG49_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOQ16707.1"
                     /translation="MGYWNTRGLRGSTFEELINYTNDSYRSKGLGLIQKIPTPIKPVE
                     INQANRTITLAYFEQKSTVDYIGVVQSIPICFDAKETTRKSLPMNNIHPHQIRFMEDF
                     QRQGGIAFLLVYFLAFNKYYMLPFHILKAYWDKAQNGGRKSIPYEAFPKDLEVFSKEG
                     AFVHYLETLNILLSKYKNDICL"
     gene            18749..19927
                     /locus_tag="PLG49_04975"
     CDS             18749..19927
                     /locus_tag="PLG49_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16708.1"
                     /translation="MRVTKWNKMLINFFKPYFDYFRMSLEEQAQYLESQKKYRLARKK
                     YIQLKNYEKVIELSRLIRDYKSVFLYQVKNNQTYEAMQTAELYELYQLGAPLCEKQGA
                     IIKAAHMYSKFDYIKAASLYKQEKIWDKAAECYLKANQWIRAIDCLDEIKTKEKANEI
                     YEKIENIAAELIKKENYEEALKIYVRINALEKALETANKINDKKASLMLYEKLAEKAL
                     KEKDFEKACLYYEMYDSSKAFKLYLQNNDIPNAARLLMEQEKWEEAIHLYLKNRQEDK
                     AIEIARQKNNYDILMDYYKSHKNYEKISWIYDITHQADEAVDYFKRENQTDYLIYFVK
                     QLKASKTAEILKEIGHFEQAARYYLLDNNEEECINCLQLAGKSPKEIKDYLFIKNYPA
                     "
     gene            complement(19924..20913)
                     /locus_tag="PLG49_04980"
     CDS             complement(19924..20913)
                     /locus_tag="PLG49_04980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ16709.1"
                     /translation="MKEIIIGEKEANQRLDKFLMKYMNQSPKAFVYKMLRKKRIKYNG
                     KKAYGNEKLISGDIIQLYLSDETINRFQKEKEIKETRQSFLVIYEDENILICNKPLGI
                     LVHGSRDKEKDTLMDEILFYLAQKGEYSPSTSIAFTPAICNRLDRNTSGIVIVGKNLM
                     ALQGMNQMLKDNKIKKYYLAVVKGIVKNSGVLKGYHKKNKKTNEVKIFKDAIEDSKYV
                     ETHYKPLCNNGKYTLLEIEIITGRSHQIRAHLSSIHHPVIGDLKYGNEEVNKYFREKY
                     HLKHQFLHGYKITIEDAVKGLEYLKNLSFRADFPKIYKEICKGENLNLPDYLH"
     gene            complement(21021..22502)
                     /locus_tag="PLG49_04985"
     CDS             complement(21021..22502)
                     /locus_tag="PLG49_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOQ16710.1"
                     /translation="MKCKKAFSLLLALLMLISVTTACSKAEWGYFNLSKEISQLERYA
                     TVGEFDINIDEIDSGGEVYKKLFDKYSFMYEIKVDMKNFIIDCDYYAKDKANGHIISV
                     MNLKCDGNNYYVKLDGFINLIADFTDIEESNNLSEIMGDAEYISCSKEELTELLALAY
                     GDDDLAEQMTSLYFNFGTSAKQNSKYLKLIEDAMKNVYDNYETGLIKQNGNKYSFSLT
                     AEEALPVLISFVNYSIDNIEKLGPTLKSAIDIFINPLFTALGIRSEDLNLKDGVDIIV
                     TEIGANKEEFKEQFNEMFALNEKELKAVMGNSKIDYTLEKTSDNKYNQTINLVLNFND
                     RDTNFAIFKGKIKANTSTYAINPFSVEIPEEKTISVNALMKELGIDPDIYFATDFTGK
                     SVTIDLDSGFAFSGSGFNETVVNVKIINGASYLPLRAVGELLGEDVMWDNDKKQPYIV
                     KNGNSIYLNAKVIGNASYVPMRELEKVGYTVYWEAQTNMVKIF"
     gene            complement(22597..23163)
                     /locus_tag="PLG49_04990"
     CDS             complement(22597..23163)
                     /locus_tag="PLG49_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16711.1"
                     /translation="MAKVIQEADYIQKRKKKYLTYTLIFFVIMMMIFLIGFIITRTRK
                     NIFTVIAAVFTLVVAQYGVQFFSIIHFKDGDKKIAEVISNLPEDYIVWNSALLGDQIG
                     MTLIEHIIISDKEIICLYDVKNKHYQKNLDTMKRIAQNKGLKEYLKFIKIEEEQLEKY
                     IEQWSQYEIENHKKQEEILNALKTAAIV"
     gene            complement(23284..26205)
                     /locus_tag="PLG49_04995"
     CDS             complement(23284..26205)
                     /locus_tag="PLG49_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ16712.1"
                     /translation="MKVNSKIHGFILIEEKEIKEINSIARLFEHEKSGAKLLSLQNDD
                     ENKVFSISFRTPPHDSTGLPHIMEHSVLCGSRKFPTKDPFVELAKGSLNTFLNAMTFA
                     DKTMYPIASTNDKDFMNLMDVYLDAVFYPNIYKYPEILMQEGWHYELENEEDEITYKG
                     VVYNEMKGAFSSPESILFRKIQESLLPDTPYGVESGGDPDFIPELTYEDFIAFHKKFY
                     HPSNAYIHLYGNGNLEEQLEFIDREYLSHFERQKIDSSIELQKPFDEIKEIQADYPIS
                     MEESQECKTYLSLNYVIDRSTNPEIYLAFQILEYLLLETPASPLKKALLEAEIGKDVF
                     GSFDNSIQQPIFSIIVKNSDIDKKEQFVQIVKDTLAQLVKDGIDKKLVQAAVNAKEFS
                     LREADSRGYPKGLIYAIKSMSSWLYDDAPFMHLCYEASLAKIKESFNSSYFEALIENY
                     LLNNQHASLLILSPKKGLLEEKMKKTREKLAAYKAKLSKEEIADLIKKTKELQKRQMT
                     PDDPKDIEKIPLLSLEDIKKEAEKLPQVEKEEQGIKVLFHPLFTNKITYLNLYFDLKT
                     LEQDLIPYGGMLVGILGKMSTENYDYGDLSNEINIHTGGISIHADTFGINGSDKEYES
                     KFIIKSKALTEKVPQLIKLLKEIIDLTKFDDEKRLLEIIREMKSRMEMNLYAQGHVVA
                     YERLMSYFTEKGAYNEQLKGFSFYQFIVDLEENFQAKKVDIISNLKKAASAIFNINHL
                     IIGLTSEEKDYKVFQDNLSLLTENLNKEKRVKQNYTFNITNKNEGLMTQSDVQYVAKG
                     YNFLPLSYNYSGSMQVLKIIISLDYLWNKVRVQGGAYGAFFNVSRTGNIYFASYRDPN
                     LKKTLEVYDAAADYIQNFDASERDMTKYIIGTISRYDFPLTPSMKGEKAVSLYISQIS
                     QEDIQKERDEILNTKKEDIIQYRQMLKEITSKNLYCVMGNKEIIKENSDLFDSTIDVF
                     K"
BASE COUNT         8368 a         5097 c         3483 g         9329 t
ORIGIN      
        1 acatgcatcg tgtgcccaaa catgtctact ggctggactt cttcgacctt atagcctttt
       61 tccgtaagaa tctttaaatc cctggccaga gttcccggat cacaggaaac atagacgatt
      121 cttttgggag ccatttcgac catagtttct aataatcttt catcacagcc ttttcttggt
      181 ggatctacaa cgatgacatc cgctttgatg cctttttctc tataaaattg aggaattatt
      241 tcttctgctt ttcccacaaa gaattcaaca ttatctacct tgttaatttt tgcatttctt
      301 ctggcatctt cgatggcggc ctcgacgatt tctaccccat ataccttttt agctttttgg
      361 gcaaggaata aagaaatcgt tccaatacca cagtaagcat cccacacggt ttctgctccg
      421 gtaagaccgg catattctaa taccttttca taaagaacct ttgtttgtat aggattgacc
      481 tggaaaaagg ataaaggaga tatttcaaat ttgatgtctc caatataatc actgatatag
      541 tcctgccccc ataaagtggt tatttcttct cctaagataa cattggtttt cttcgtattg
      601 tgatttaata caatactggt catatgagga attctaatta attcattgat taatttttct
      661 tgatggggaa gcttttttcc gtttataata atacaaacca taatttcatt agtataaaaa
      721 gccgttcgaa taagaatatg acggatcagg cctttatgtt tttcttcatc atagaccggt
      781 attttattgt cttcaatgaa tttccttatg atcaaaagaa tctgatcaca tacagtatct
      841 tgtattttac agctttgaat atcaatgata tcatggcttt taggtgcata aaaaccgatc
      901 tggacttgtc ctttttttaa acctacagga aactgtgcct tatttctgta atgaaaagga
      961 ttttccatgc ctaaagtcgg atgtacttgt atattgctaa gctttccaat acgctccaga
     1021 ctatctgtaa ctctttttct tttatattct aactgtgcct gataatctaa atgctgtatc
     1081 tgacagcctc cgcacctttt ggtataagga cagggagctt tcactctgta aggagaagct
     1141 ttaataatcc ttattaattt ccctattgca aagttcttct ttacttcaac aatttttgct
     1201 tctacctcat cttctggaag cgccccttct acaaacacag taaaattatt aattcttccg
     1261 ataccttctc ctttagaact taaatcttct atagtcatca aaaagttggt gtttttcttt
     1321 atggctagtg aatccattaa tcttcctcca atttcagtgt aacttatctt gatttaataa
     1381 ttcccgtatg gcttttgcca gtacttcatc gctgctctct ttcagagctt cttcaatgat
     1441 attctttttt tctggtatag attcagaagt gttattattt tggttaatta atcttctgtt
     1501 tttatcttct tttttattca tcctatcaat cctccccata aaattcgcat accgttccct
     1561 tacatataca atatcggcat tttacatagg aaggattgac attttgttcc ccgtttttta
     1621 acatatctct catatccgct aaaagatttt ttactttccg ccgcaaaaac catgtatttt
     1681 ttataataaa catgtaattg ttataaatca agcgcccttc tttaggtctt tttttataca
     1741 cgtcttcgat aattaaaaaa taagccgcca actgcattaa atccccttca tagggctgta
     1801 tattatcttc tttaatcgta gcactcttta attctactgg tatcaaattt ttacttaaaa
     1861 ttttttcaaa aatataatcc ggtttgcctc ttaaatcata tttaggggaa tataaaacct
     1921 ttccgtactc tacattggga tcttttttgc tctcgtgctg atctgtatag ataagccggg
     1981 cagcccttat cccgagtttg ggttttttta caacaatatc gggtcttctt ataaaaaaaa
     2041 gagatattac taagaaaaga aatatccctg gtaaaatata gatataatca taaaacatgt
     2101 tttatcacct gataaatatg aatgaagaat aaaattgaaa gggtaatcca aaaaatcttt
     2161 agtaaaaact gttttatttt atatgcaaaa tggtaattct tgtgaaagcc tgtgccctta
     2221 ttaaaatgac ttaaatccga atcctcataa ccataatatt catttctgat tttagccaat
     2281 tccctgagct ttttactgcc atacagtctc tgataatacc attgataagg gcaatataca
     2341 aatttattaa tctctcctgc agaaatcgta ttgcttttat tttctatatg gtcctttcgc
     2401 atcaaaaatc acttttcctt atatttctcc caagcatccg gttatagcca acccattccc
     2461 cttgaaaact atctgcctgg attgattctt caaaaagctt aattttggca tctgaattat
     2521 ccgcacagtg aataatcatc gcttctataa ttttaggacg cttaggagaa ccaaattcaa
     2581 attctccatg atgggctaaa atacagtgct taatcagcaa ctccaaactt tcaggaaagt
     2641 cactgatttt tttaattttt tctccgacca tttcagcccc catcacaata tgtcctaata
     2701 attgacctga atccgtataa tcattggatg gaaaatccga tagttcttta atctttccaa
     2761 catcatgaag cattgctcct gcaatgacca aatctttatt gacctgctta tattttgaag
     2821 ctaaaaattc agctatggaa acaactgtta aagtatgctc caataaccct cccatataac
     2881 tatgatgaac actttttgct gcagaatgat ggataaattt attaaaaaat tctgcatccg
     2941 atagaaaaaa agactgcagc aactttttta aatatatatt ttcaacttta tcaatataaa
     3001 tttttagctt ttcaagcatt ccttcaatat cctggtctgt acagggaata taatccatgg
     3061 ggtcatattc cccttccata cttaaacgta atcttctgat atttatctga tattctccct
     3121 gataaacact taccacacca tctattttaa caaaggaatt ttcctcaaat tcatcaatat
     3181 cacaattaat atcccatatt ttaccgtcga caaagcctgt tttatcttga agctttaatg
     3241 atatgtaagg cttaccgttt cttgatttta aattctgctt ttgcttacaa agatagtgac
     3301 cgattatttg ctcaccatct cttatatcct ttaaataacg cattaaaatc ctccattcaa
     3361 aaagttcaaa attcctttat ccgttgttat gattatacat aaatttttat ctttatgcca
     3421 gtattccaat aaaaaacata atagaagggt aaaaaataat aatctaaaac aaaataagtt
     3481 ttcaattttt caaaaaacac aaactcatca taaaaaataa ggcggtaaaa ccgccttata
     3541 atctttattc agaaggtttt tgctgaagcg taacggttac tgtcttttta taagagccat
     3601 ctctaatcag tttaacttca attttatctc cgactttatg tgactcaata atcttagtca
     3661 gttgcttcat gctggtgatc ttttcaccgt caaattctat aataatatct cctgcctgaa
     3721 taccagctgc atcagcaccg ctgccttcaa taacttgtct gactaaaaca cctataggaa
     3781 tctcgtataa ttctgagaca tcctcattaa tatcttgtcc catgattcca agataaggtc
     3841 ttgacacaga acccttatta accaattctt ctataattgg ctttgcaaca tttataggaa
     3901 ttgcaaaacc cataccttct acagaagtgt caactaattt gatagaatta atacctataa
     3961 ccgttcccct ggaaccgact aacgcacctc cactgtttcc cggattaatt gccgcatcgg
     4021 tttgtattaa ggtcaattct ttatcagcaa tttggatttt tctatttaaa gcactaataa
     4081 cgccggcagt aaccgtatta tcataagcat cacctaaagg atttccgatt gccactgcta
     4141 attcccccac ttctaactca tcagaatttc caaaaggtgc tgctttaact tttcctctaa
     4201 tttcatttgg aagatcttcc tgtaatactt ttataacggc taaatcgctt ggagaatcca
     4261 gccctacaac cgttgcaggc acagaataat tgcccagaaa tgtcactgta agcttattat
     4321 tattcgcaac aacatgataa tttgtcacaa tcagcacttc tttgtcatta acgtggaata
     4381 caatgcctga acctatccct tcctgtaaac gctcctgatt ccaccaatcg gttacaacat
     4441 aagtatttct gatggaaaca acagaaggac cgattttttt tgcaatttcc gggataattg
     4501 attcatattt aggcaaattt gaacgagtca tattttctgc attcatttct tcagcttttt
     4561 gattgtcaaa gctaaaaagt gcttgatttt cttctgtttt cttatctgca ttaaaaaatc
     4621 tttccgatac ggattctaaa gcaggctgaa caaacaggcg gttgacagga cctgcaagtc
     4681 caattcccaa acctacacac agaccgccaa aggctactaa aagcacgatt gctgcagctt
     4741 tcatcttatt caatttatgc ttcttaactg gcttgatggt ctgagaataa taaggaacgc
     4801 tattctcaat tccgggagca tgacttatcg cttctttagt tatttcaggc tctgcttttc
     4861 tgtcttcttt tgtaacttcc tgatttagtt ctgcatcggt atgctcactg acttcaggat
     4921 tcatttcttg acttaaacca tcttctttta tgatttcatt ataaactcta tcttcatcca
     4981 tttcttttac ctcccttttt attttttcgt ttctaatctt attataaaaa ttagctttga
     5041 ataaaatatg aacgatttgt aaatattaat agattttatg tgaacaaaca gccttatttt
     5101 taataaatag aaattgtaaa agtaaataca acgccatctt caacattttc tacttttatc
     5161 gtttctccat gatatttaat aatttgttta acaatagaaa gccctaaacc catacccgtt
     5221 ttatcttttc ctctggactt atccgtttta tgaaatctgt cccatatata tgggagatct
     5281 tcttcaggaa ttttttctcc actatttttt acagaaacca acagcttttt accggataaa
     5341 gttgtttcaa cccaaatact tccttcttct ttgacaaatt taactgcatt gtctattaaa
     5401 ttgtatagaa ctctctggat ttgttcttta tctgcaaaaa ccaggctttc ttcctccgcc
     5461 agaatcaaat acaattttac tttcttttct aaaatccttt gttcaaattg agctaaaacc
     5521 cttcttacaa gatcattaaa ctcaaattta tctttattaa gttctatctg accgctttcc
     5581 atcttagtca aatccataat gtcattagtc attttagtca gtcttcttgt ctcatctaaa
     5641 actattttca aataattaga ctgcttttga ggagggattg tcccatccag aatagcttgg
     5701 acaaaaccct gaatagatgt taaaggagac ctgaggtcat gagaaatatt ggcaatgaaa
     5761 gcttttctaa gttcctccaa atgattaagt tcctgcgcca tatagttaaa acttgaagcc
     5821 aattgtgcga cttcatcttt tccttttata gaaagctttt tatcaaaatc tcctcccgct
     5881 ataaccttag ctgcttcatt catttcttta agagggttgg taatacgttt cgataaataa
     5941 taaataaaaa taaaagagat taaaatagat atgcccaaac ataaaacggt tatcctgaat
     6001 aattctccta ccgttttttg aatttcagga ataggtgtat gcatgaaaag agctccatat
     6061 actttatcac caagaattat aggataacct acagttaata ctggctcctt aaagtagcct
     6121 ccaaaacgac ccttaacagt taagattttg ccttcaaaga cttcattgat ttgttccatg
     6181 gttaattgct gacctaacca ggcttcattt tcttttctcg atacttcata gattcttcca
     6241 tccgcatcaa caatccaaat catagcatct aaatatttat ccagtacttg catctcaaaa
     6301 ctcaactgtt ccagatcaat gacgcctgta taataggctt ttgcatattg catcgttatt
     6361 ttttcacctt gttcaagcat tatatttttc ttttgatgaa taaaataact ttcaaagact
     6421 tgagatagta aaacaccaag caaaataaaa ctcatcagta taataactgt aaaagaaata
     6481 aacaatctgc taaataaaga tttatacata aggtcacttc ctatcatatc catattcaaa
     6541 ttaaatgata aagtatttta aataagttat gcttttattt tatatccttt taaattcaga
     6601 atcaataaag atgaatgatg gatacgacaa gaagacttct actgtaatat atagataaaa
     6661 taaccttctt catcgactat ttttttaaaa ttttcctcca aataatttct atattcgccc
     6721 gtatcattat gaatccagcc cttcatagga acaaaatatt ttgctccgtt ttctatataa
     6781 tattgaattt cttcaattgg atcctttggt atatgatcat aataatcaat attaaccctc
     6841 caaccctttc tttcgcttgc attaatcagg gccggatctg gagtacctac tacaattaaa
     6901 tcttctgcat ccgtatactt tttaaccata ttggcttgtt ttaacagatt acttttttcc
     6961 tgataaagag gtcgtaccct caaaaaagaa ttaaaagcaa tactaaaaat gatagccgta
     7021 acaaataccc ctccatattt atgttcccat ataaaataca aagcttttga agcaataata
     7081 gccattaaag gtcctataaa aatcaaataa taatttaaac ggataacagc tacaatgaaa
     7141 acaagttctg ctaacatagc caaaagccag aatacaatag gaagctcttt ttttaatttt
     7201 aacatcaaaa ggcctgttaa aaataataaa agaacttcca gcgtaaattc ctgaggaagt
     7261 tttgtcttaa aaaaatcgat agcttctttt gtaaaaatat ctgtaaacaa acgcttaaag
     7321 atatgctttt ctgctatttt atttacaaat ttaaattctg caatatagtc catccattta
     7381 aaatacaaga aaggacttcc taaagaaatc acagcaaata tatataattc tttaatctta
     7441 taaatctttt ctttatattt ttctattgcc ataacaaaca ttggaatacc aataaaaacc
     7501 gttggaatct tttgagatat tgccagggcc gtaaaaaatg ctccagaata aatatacttt
     7561 ttttcttctt tataaatcca tttcagataa aaataaaagg cacctaaata aaagaataaa
     7621 gcagcagact cgggcataat tgcacgggaa aaataaatat taataggaat taaagcataa
     7681 aggattaaaa tcatataaga ttgtgcttta gagtaaaaaa atcggccaat aagatataaa
     7741 aaaatgcatg aacccataaa aaaacttata ggaacaaccc gagcaaatat ataatgatac
     7801 ccaaataatt tatataaaat tgcaatgata aaagttgtaa tttgaaattc cagttggacg
     7861 taattgggca agggtccgtc atagttaaat tggggataaa aaatattaaa tctttcttca
     7921 acaaaattgc gggctatcga ttctgtatcg gactgtctcc agttttctcc ttgttcataa
     7981 ggcgaattgc tgatataagg tattctcagg agcaaaataa aaaaaagaat aagtataaat
     8041 atgcatttat ctttaacttt atccataaac aacgccaact cctataacaa ttaaaagggt
     8101 tcctaaaatt tttttaaagg tcaagggctc atcaaaaatt aaaacagata taaacaggat
     8161 aataatatag tttaagctga ccaaaggata acttagactt aattcgtttt tgcttaaaac
     8221 cttgatccac aatagaaaac taattccaaa taaaataagt ccaatgatga tctctggtat
     8281 tttcataaga tttatgattt ctttatgtat tgaaaataaa gttaaagata agtcccctga
     8341 tttaacagca ccttttttta aaataacctg gcccaaggcc cctaaaacaa cagataataa
     8401 agctacagat acatcattca taataaccct catttcttaa ttaccatatt cataaagcta
     8461 atgaggcttt ttaacccttc tcagtaaatc aaagtaacct aatatggtct caaaaacatt
     8521 catcttactg gcactcgctt ttaaatcata ctgtagttta agcggatatt ctgctgcttt
     8581 aatcccaatc ttactgaact tagctaaaat ttcagccatg cagtcaaagc cgttcgtcgt
     8641 aattaactga ttgttatata actgcataga acgtctgaga tatcctacct tatacaccct
     8701 aaatcctgat gaataatcat taatatttct tatattaaaa aatagcttaa aaaagtattt
     8761 tgcccctctg ctgtatagtc tccgtatcaa tggaacacct cgttcttctc ctccacgtac
     8821 aaatcttgag gcaataatta aatccaggtt ttcttcctta atcctttgaa tcatattgat
     8881 gatttggtaa ggattatgcg tattgtcagc atccatcgtt actaaaaaat cattgtcaca
     8941 agctttttct ataaccgcat gaaatatagt attgatagca gcccctaaac ctaaattttt
     9001 ttcatgataa agccctttta taaaatgata actatcacta aatgcttcta taattttttt
     9061 tgtacgatcc gtacttccat catcgactac tatgacttca agttcttcat caatattatt
     9121 tgaaaaatca ataatttttg ataaaagctc aggaagagat ttttcttcat tgaatgcagg
     9181 aaggcctata aaaattttat ctgccataaa cttccttctt tccttaaata aatgcttcaa
     9241 aaagattaaa aatttgcttt atacatatta tgagcagtat ttattttttt atgaatttat
     9301 aggccacctg atgttgaaag aatagttttt aacaatattt taagaaaaat aaaaaagtat
     9361 cccctggata ctttaataac tatacttttc ttcgtcttat ttctatactg tttaataaat
     9421 catggatcct ttcaaaaatc aacccagcag caaaccaggc gggggcataa tccaagcgga
     9481 taaaaccatc aattgaaaaa gtactgtccc cgtaatccca cggacaaaca cctataatat
     9541 tttgcagagt ccagccggcc aaatattcaa tgataaaaat taaaagggta taaacacctc
     9601 ctcttattat aaccggccag gctcttattc tgtcgtgaat tggttcaaaa agaattgcca
     9661 gaccgtagat cggaaacatc catatatagg ttcttgcatt cagataaatg tctccatcca
     9721 gaaaggaacc aattcctgtc catatgattt ccatgcacca tccagctaat ccatatataa
     9781 taaatctttc tatcataatc attatttttt gttataatga cagtaaatat ttattattaa
     9841 gcagttccca tatattctca aatcatttac tcatttattt ccagacgata tagtctctca
     9901 taaggagaat ctttcttaaa tgttttaacc tcctcaataa atacccagcc aaatttttca
     9961 tataaaccaa tgtgccttgt atgcaggtac aattcagtta aatttgcttt tctggcattt
    10021 tccttaacgg ttttcattaa ttccctgcat acattcttcc ctctgaattt tttatcaaca
    10081 tagacattaa tcaaccatgg ataaatatca ggcctgcttt ctaaatcatc aaacattgct
    10141 aattgataca taccagccgc ttcttctcct attaaagcga cataggtctg tggcagtctg
    10201 tctttattta atgaatgctc taaattacat ctaacttctt cataactcct cccgtcccgg
    10261 ataccccacc aattgtaatt ccattcgcat attctttcaa aaacagggtt gtttttatct
    10321 tctaatctta ttattttcat atgtttcttt cctctcagac taagttcctt tttaatataa
    10381 tacacgaaat taagactgaa aaaaagaatt ctacatattc aaacgcctgt atgtagaatt
    10441 cttttttcgt gtaaatctta tttattcttt atcttctttt tttcaattca ttgcatatat
    10501 cttcccactg aatatctgtt tgcaccatta caaccattaa atgatataat agatcggaga
    10561 tctcatagac aatttcttct ttactgttgt ttttagaagc aatgataact tcagcagttt
    10621 cttctcctac ctttttcaac atcttatcaa tacccttgtc aaaaaggtag ttggtataag
    10681 aaccttcttt gggatttttt cttctgtctt caatcacttt ataaacttct tgcaaaatct
    10741 gaaatgtctc attgccacat ttacattttt ctttatccac taaatcttct cccttaaggg
    10801 aacggtaaaa acatgaataa tttcccgtat gacaggcatt tcctacttgt tcaactaaaa
    10861 ttaaaatggc atccatgtca cagtcaaagg aaatctcttt tactttttga taattccctg
    10921 atgtttctcc tttaagccat agggcttgcc tgcttctgct aaaataatgt acttttcctg
    10981 tttcaaacgt cttctgaagg gcttcccaat tcatatatgc catcatcagt acttcttttg
    11041 ttctgacgtc ctgcgtaata acaggaatta agccctgtga atcaaattta atattattca
    11101 ttaagtccat cgtatttccc ccacttatta ttaacattat tatatcttta taataactta
    11161 aattcttact ggaacatttt tttctttaag ataatgcttt aaatctttta tttctatttc
    11221 acggaaatga aataaagatg ctgctaaagc gccatcagcc ccacctctgg tcagagcttc
    11281 ataaaagtct tcttttttac ctgccccacc agaagcaata acaggaatgg aaacagcatc
    11341 tgcaactgct ttagtcattt ctatgtcata tcctgcttta gtaccatcaa aatcaatgct
    11401 ggttaaaagg atttctcctg cacctctttt ttctacttcc agagcccagg atacaacatc
    11461 tactccagta ctttgctgac caccgtttat ataaacctcc cagccttctt cattttcttt
    11521 tttcttagcg tctatggcaa taacaatggc ttgcttccca aattcttctg ccgcctggga
    11581 aatcagttcc ggtgacttaa aagcagccga tccgatggat accttattag cgcctgcagc
    11641 caatattttt ctaatgtcat caacggttct gataccccct ccaacggtaa gaggaataga
    11701 aatacgttct gctgttttct taaccacatc aatcataata tctctgccct ctgttgtagc
    11761 agtaatatct aaaaaaacca gctcatcggc catggcttca ttataaatag cggcaatttc
    11821 cgcaggatca cctgcatcaa ccaaattaac aaaattaacg ccttttacaa ctcttccatg
    11881 attaacatct aaacacggaa taatcctttt ataattcatc taacatcctc cctcatataa
    11941 gcttattgct tcttttaaat ctatacttcc ctggtacagg gcttttccaa taataacacc
    12001 ttcagcatta atcttgctga ctttctcaag atccgttaag cttgctacgc ctcctgaggc
    12061 aataatatcc attcctgttg catcaattaa ttccttagtt gcctggatat taagaccaga
    12121 aagcatccca tcttttgaaa tatctgtata aacgattgtt ctgacaccca tttttttcat
    12181 ttgaagggcc aggtcaacag cagatactga actaagttct tcccatcctt ctacagcaac
    12241 tttaccatct ttagcatcaa taccaacaac aattttatct gctccaaata aatcaatggc
    12301 ttctttaacc agttcaggat tttttacagc tgccgtccca aagatgactc tggcgacccc
    12361 taaagaaagt ctttcttgaa ttgcttcaag gtttcttatg cctcctccgg tttgaatggg
    12421 aatattaact gcttttacaa tctcccttat aacttcttta ttggcctgat taccaagaag
    12481 cgccccatct aagtctacta aatgaatata ggaagctcct gcatttttcc atgctttagc
    12541 cacttcaacc ggattttcat tatatactgt gaccttgtca aaacggcctt gggatagcct
    12601 tacgcatttc ccgtctttaa tatcaattgc tggataaatt ctcatcttaa atcccccagg
    12661 ttaaaattat tttatctaag cttctaaaac accttttgtt gaaagaaccc cttcaattct
    12721 tgggtctgtt tgaatggctt cattcagagc cttagcaaaa gctttaaaga tagcctctat
    12781 gatatggtga ttattgcgcc cgcttaagac tttaatatgt agattaattc ctccatggat
    12841 gcataaagcc ctgaagaact cttcgaccat ttcagtatcc atatcaccaa ctttttcaac
    12901 cgtaaattga gcatcgaaga caagatatgg tctgccagac aagtctaatg cgcataatac
    12961 cagggcttct tccattggta aaataacaga accgtagcgc tttatgcctt ttttatctcc
    13021 taaagctttt gcgaaaactt gtccaaggac aattccagta tcttctatgc tgtgatgaca
    13081 atcgacgtct atatcccctt taacagctat atccagatca ataaaagcat gttttgaaag
    13141 ctgactaagc atatggtcaa aaaagcctat gcctgttttt atgctgtatt ttcctgtccc
    13201 attcagattt atagcagctt tgatgtcggt ttcgctggtc ttacgatgaa cttgtgcttt
    13261 cctttccaac atttttgatt cccctttatc atttaattat tattttggaa tcttacccgg
    13321 acagaatttg catgggcagt aaattcttct gcttcagcaa agcggataat atcgtcactt
    13381 aattctttta aactatcttc agaaaaataa atcacactgg attttttggt aaaatcatct
    13441 acgttaagag gagagaaaaa tctggctgta ccactggtcg gaagaacatg atttggacct
    13501 gccatataat cccctaaagg ttcaggtgaa tagtgaccta agaatatcgc ccctgcattc
    13561 tttattttgg gcaacagagt catagattct ttggtacata gctctaaatg ctcaggtgca
    13621 atttgatttg ttatttcaat tgcttcttct atgttattca caatgatgat tgccccatag
    13681 tccttcaatg atttttcaat gatggctttt cttgaaagaa gttctgtctg acggactaat
    13741 tcttccttaa ctttatccgc taaattcctg ctggtcgtta tgagaacaga agatgccatt
    13801 tcatcgtgct cagcctgtga caataaatcg gcagcgacaa aaacaggatt ggctgtttca
    13861 tctgccacaa caagaatttc actgggtcca gcaatagaat caatgctgac atagccaaaa
    13921 acaattctct ttgctaaagt tacatatata tttcctggtc ctacaatttt atcaacttta
    13981 ggtatgcttt ctgtaccaaa agccagggct gctatcgcct gagcaccacc tattttataa
    14041 atttcagtaa ctccggcaat ttttgcagca actaatatcg ctggatttat agtgccatct
    14101 ttttgaggag gtgtcaccat aacaattctt ttaacaccag ccacttttgc tggaattaca
    14161 ttcattagta cagatgaagg ataagctgcc ttacctcctg gaacataaac ccctgcgctt
    14221 tccataggtc ttacaatttg ccctaaaatg tttccatctt catcgggact aatccaactg
    14281 tttctttttt gcttttcgtg aaactggcgg attctgtctg ctgctttttc caatgtctct
    14341 acaagctcag aattcagttt cttataagcc aaattcattt cttcttcgct gactttaagg
    14401 ctttgacttg ttagacaaac cctatcaaac ttttgggtat attcaagaag agctgcatct
    14461 tttctaattt gtacgtcttt tacaatctct tcaacagtct tttgaatatc tttataatcc
    14521 aattgtgatc tttttagtaa ttctttaatt aattcattgg ctttataatc ctgattatca
    14581 ataatccgta tcattttctc acctctattc attgtcaatg atttttttca ttttttcaat
    14641 taaatttgtg attctttcat attcaatttt catgctgaca cggtttacaa ccatccttgc
    14701 tgaaatagga cagatttctt ctaatactac caaaccattt tcccttaaag tgctccctgt
    14761 ttccacaata tcaacaatca cttcagaaag acctacaatg ggagccaact ctacagaacc
    14821 atttaattta ataatttcga cattttgctt cttattgaaa tgaaaatact cctttgcaat
    14881 attggggtat ttcgtagcca ctctgaaatt ttgaccaaat ttcagggttt ctttcatttc
    14941 ttccggtccg gcaacaacca ttctgcaatt accaatgccc aaatttagta cttcatataa
    15001 attacgtttt tcttctaata aagtatcttt cccaactaca ccaatgtctg ctgccccata
    15061 ttcaacatag gtagggacat ccgatgcttt tgccagaaaa aattttaatt ttaaatcttc
    15121 attggtaaaa ataagctttc tggatttttc tttcatttca tcacagctaa aacctatgga
    15181 ttctaacaat tccattgttt tatctgctaa tctcccttta gcaagggcaa atgttaaata
    15241 tctcaatttt cttctctccc ctcagatctt tcaagcaact gatctactgt agttttcacg
    15301 ctttgattgt tttttaagtc aatcagttca atcttttgct catctataaa atacaaaata
    15361 ccatcaatat ttttagagcg ggcatattct ttttgagctt caatatcacc atccaataaa
    15421 ctgttttcaa tatacatgcc ttctcttcta agggtatctg ctgtcatcag ggcctgtttc
    15481 ctgccttctt tattataaac caataaggta tctaccttcc aaacgggtat cttaatcttt
    15541 tgtctgttaa ttgcattaat taattggtct gtcataatcc caaaaccgac agctggacaa
    15601 tcatgaccaa attcaccgag taatctgtcg taacgaccac catctaaaat ggaagaaccc
    15661 gttccaaaag tatatcctct aaaaataatc ccagtataat aattaatccg gttaaccatc
    15721 cccagatcaa aagatacata tttttctatt ccgtaatcac gcaaaataga ataaacattt
    15781 tctaattttt ctaatgctct ccttgctctg ctgttttctg ttcttacttt ggttttttca
    15841 ataatatcaa taggaccgca aagtttgggt aaatctaaaa ataattcctt caaatttgga
    15901 tttaattgaa attgttcaat aaattcttca acgcctatga aatctttatt ttctattaat
    15961 tgtcttaact cttctccttc atcttcactc aagcctgctt cctggagaat gcctttaaaa
    16021 aactcgacct gacctaaatc aatttgaaaa tcatttaagc cagcagccag taaactatga
    16081 atggctacag caatcacttc tccatcggct tcatcggaat ttattcctaa aagttcaaca
    16141 cctgcttgag aaaattcacg atttttcccc tgtaaacttt cataaaaacg aaaggcatta
    16201 cccagataac aaaggcggac tggctttctt tcttctttga aaacagtcgc accaatcctt
    16261 gcaatagggg gtgtcatatc tggtcttaaa gccaatatat ccccttctct gtcagcaaat
    16321 ttatagatat gcttttgctg taaatcttct tttctttcta gaaaaacatc gtaatattca
    16381 aacgtggggg tttcaacatt ataatagccg tatcgattaa aaacacattc tattcttttt
    16441 tcgatttctt tttttttggc acattcctct ggcagcagat ctctgacacc ttcaggcgta
    16501 tgaagtttat agttcatatc ataaccgctc ctttagtttt gtaatgcttt aaagttttaa
    16561 agttataaat cgatgaattg tttatattat attcgcaaat aaattatttg tcaatttaat
    16621 attaaaaaaa agacatgccg gatttgatat gctccttttg tagcaaaccg tttaattaat
    16681 aataaatgct tactacaaaa gtatatcatt ttccacatgt cttttattga acttttataa
    16741 ttttttcccg atcattaatg ataatattta acccattatc ctttaccaag ttgtttaatg
    16801 aataataaag agaaccatca atgataaccc cttcttcttt ttttaaatta aatatttgag
    16861 tattccattc atatcttata actttctctg aaatataaaa ttcctcacct tttttataaa
    16921 gaggataata actcacaaac cagtaatttt ctttttgtcc aatgtgctgt gtatcaagtg
    16981 agtcggtaaa ataatctgtt ttaactgcta atgcataagc ttctgaaaga ttcctgtgag
    17041 ccgttatttt aaaattatca taaccttctc cttccggagc tgcttctata ttatcaaaat
    17101 ccatataatt aactgctttg atggccggat aataactttt tactcttgta taaaaatcct
    17161 ttatcttttg agctgcttca tcaacataat aggcatgatt atgcttagag taatgggata
    17221 tagccaactg agatatcata ataggctttt tatcctgaaa taaaaaataa aagaattcta
    17281 tatcctgaaa aattttttca ttgctgctat ccagttcagc taaatttttg tcttctatca
    17341 tatcatagac gtggacagcc acccagtcaa ccgcatcatc tccaggataa taaagcatag
    17401 aatcttttac atttgtatga ttgacacccc agacaaaagc tacattgggc gcatattctt
    17461 taaaaatttc cctcgctttc ataaaaaaat ctttatattc ttttgcattc ccatattgtt
    17521 gaggatccgg ataccattcg acaaacattg ggatataata ctctccaaag gctttggccg
    17581 tttcttcaag aagatattcc tgataaggat aatgtatgga tggggggtga agtataacaa
    17641 gaggcgtttt catttttgcc gtacattcca aaacccaatc tataggaaaa gctttaccaa
    17701 gtttcaagtt tcttaaataa ataccatgac ttttttgtgt tttgtcttca aatatctcaa
    17761 tatcaaaatt taaatcagca ttggataaaa tatacgctcc tatataacac tgatttactg
    17821 gctcatataa acttagactt ttatttttaa gaataaataa tttataatcg cttttttcaa
    17881 acaagtcctc acttaaaggc aattcttctt cctgactgta agtcgcctgc tggatattac
    17941 tttttgacat ctgacaggca gttaaaataa agcaaaaaaa tatcatatga atgataagtc
    18001 ttttcacaaa aatcccttcc ttcatatgat atttttgcca ttatataata gattattcat
    18061 agacagatat catttttata tttgctcaaa aggatattaa gggtttcaag ataatgaaca
    18121 aacgcacctt ctttactaaa aacctccaga tccttaggaa aagcttcata agggatcgat
    18181 tttctgcctc cgttttgcgc cttatcccaa tatgctttta agatatgaaa aggaagcata
    18241 tagtatttat taaaagccag aaagtaaacc aataaaaaag caattcctcc ctgtctttga
    18301 aaatcttcca taaagcgtat ttgatgcgga tggatgttgt tcattggcag gcttttccga
    18361 gtggtttctt tggcgtcaaa acaaatcgga atgctttgca ccacaccaat ataatcgact
    18421 gtactttttt gttcgaaata agccaaagtt atggtcctgt ttgcctgatt aatttccaca
    18481 ggtttaatgg gggtaggaat tttttgaata agccctaaac cctttgaacg atagctgtca
    18541 tttgtataat taatcaattc ctcgaaagta cttccccgta atcctctggt attccaatat
    18601 cccatagtat cctccgtttt ttatttatat attatcataa aatagcttca atgcatgcat
    18661 taaaataata ataaacaata aagtgatgat aattattgag taattaagaa aaatatatta
    18721 tactataatt cacgaggagg aatctgtaat gcgtgtaaca aaatggaata aaatgcttat
    18781 taatttcttt aagccatatt tcgattattt ccggatgtct ttagaagaac aagcccaata
    18841 tttagaaagt caaaaaaaat atcgattggc cagaaaaaaa tacatccaat taaaaaacta
    18901 tgaaaaggtc attgaattaa gccgtttaat cagagattat aaaagcgtat tcctttatca
    18961 ggttaaaaat aatcagactt atgaggctat gcaaacagca gaattatacg aattatatca
    19021 actgggagca cccttatgcg aaaaacaagg ggccattatt aaagctgccc atatgtactc
    19081 taaatttgat tatataaaag cagcttcttt atataagcag gaaaaaatat gggataaggc
    19141 agccgaatgt tatttaaaag caaatcaatg gatccgtgca atagactgtt tagatgaaat
    19201 aaaaacaaaa gaaaaagcca atgaaatata tgaaaaaatt gaaaacattg cagcagaatt
    19261 aatcaaaaaa gaaaactacg aagaagcttt gaaaatctat gtgagaatca atgccctgga
    19321 aaaggcctta gaaacagcta ataaaatcaa tgataaaaag gcaagcttga tgctctatga
    19381 aaaattagca gaaaaggcct taaaagaaaa agattttgaa aaagcttgtt tatattatga
    19441 gatgtatgat tcttccaagg cttttaaatt atatcttcaa aataatgata ttcccaatgc
    19501 tgccagactt ctgatggaac aggaaaaatg ggaagaagcc atccatcttt atctgaaaaa
    19561 tagacaggaa gataaggcca tagaaattgc caggcaaaag aataactatg atatattaat
    19621 ggattattat aaaagtcata agaactatga aaaaatttcc tggatatatg atattacgca
    19681 tcaagcagac gaagctgttg attattttaa aagagaaaac caaacagact atcttatcta
    19741 tttcgtcaag caattaaaag cctcaaaaac ggcagaaatt cttaaagaaa tcggacattt
    19801 tgaacaggct gcccgttatt acttattaga taacaacgaa gaagaatgca taaactgtct
    19861 tcagttggca ggaaaaagcc caaaagaaat aaaagattac ttatttatta aaaactatcc
    19921 ggcttaatgc agataatctg gaagattcaa attttcaccc ttgcatattt ctttataaat
    19981 cttaggaaaa tccgcccgga aagaaagatt tttaagatat tccaatccct ttaccgcatc
    20041 ttctattgtt atcttgtatc catgtaaaaa ttgatgtttt aaatgatatt tctccctgaa
    20101 atatttgtta acctcttcat tgccatattt taaatctcct atgacaggat gatggatact
    20161 ggaaaggtga gcccggattt gatgagatct tccggttata atctctattt ctaaaagtgt
    20221 atattttccg ttgttacata atggtttgta atgtgtctcc acatatttgc tgtcttctat
    20281 agcatcttta aaaattttaa cttcattggt ttttttattt ttcttatgat aaccctttaa
    20341 aacacctgaa tttttgacaa ttcccttaac aaccgccaga taatattttt taattttgtt
    20401 atctttaagc atttgattca ttccttgaag agccattaaa tttttaccta caataacgat
    20461 accgcttgta ttccggtcta agcgattgca tatagctgga gtaaaagcta tggaagtact
    20521 gggactgtat tctccttttt gtgcaagata aaataaaatc tcatccatta aagtatcttt
    20581 ttctttatcc cgactgccat gtaccagaat tcccaggggt ttattacaaa ttaaaatatt
    20641 ttcatcttca taaattacca aaaagctttg tctggtttct tttatttctt tttctttttg
    20701 aaaacgatta atggtttcat cacttaaata aagctggatg atatccccgc ttatcagttt
    20761 ttcattccca taagcttttt ttccattata ttttattctc ttttttctga gcattttata
    20821 aacaaaagct ttagggcttt gattcatata cttcatcagg aatttatcca gtctttgatt
    20881 tgcttccttt tcaccaataa taatttcttt cataagttca cctgttcttt cgattttact
    20941 ttattattat tataaaacaa ttatattaaa aaatcgccct gatgggcgat ttttatttat
    21001 ttttatttaa cttgtaataa ttaaaatatc tttaccatat tggtttgagc ttcccagtaa
    21061 acagtatatc ctactttttc taactccctc attggaacat aagaggcatt tccaatgact
    21121 tttgcattaa gatatatgct gtttccgttc ttaacgatat atggttgttt tttgtcatta
    21181 tcccacatta catcctcacc taataattca ccaacagctc ttaaaggtaa atatgaagca
    21241 ccattgatta ttttaacatt tacgaccgtt tcattaaaac ctgaacctga aaaagcaaac
    21301 ccactgtcta aatcaatggt tacacttttg ccagtaaaat ctgtagcgaa atatatatct
    21361 ggatcaatcc ctaattcttt cattaacgca ttgacagaga ttgtcttttc ttcaggaatt
    21421 tcaacagaaa agggattaat cgcataagtt gaagtgtttg cttttatctt tcctttaaat
    21481 attgcaaaat ttgtatccct atcattaaaa ttcaggacta aattaatagt ttgattatat
    21541 ttattatcag atgttttttc cagggtataa tcgattttag aattacccat tacagctttt
    21601 agctcttttt catttaaagc aaacatttca ttgaattgtt ctttaaattc ttctttattt
    21661 gcaccgattt ctgtaacaat tatatcaaca ccatctttca aattcaaatc ttctgaacga
    21721 atacctaagg cagtaaatag aggattaata aaaatatcta tagcactttt taaagtagga
    21781 cctaactttt caatattgtc aatagaataa ttaacaaaag atattaacac aggtaaagct
    21841 tcttctgctg taagagaaaa tgaatattta ttaccatttt gcttgataag accagtctca
    21901 taattatcat aaacattttt catagcatct tctataagct tgagatattt gctgttttgc
    21961 ttagcagaag tgccaaaatt gaaatatagg gaagtcattt gctctgctaa atcgtcatca
    22021 ccataagcaa gtgctaacaa ttcagtcaat tcttccttgc tgcagctaat atactcagca
    22081 tcacccatta tttctgataa attattgctt tcttcaatat cggtaaagtc agcaattaaa
    22141 ttaataaatc catccagctt tacataataa ttattgccat cacattttag attcatcact
    22201 gaaataatat gaccatttgc tttatctttt gcataataat cacaatcaat aatgaaattc
    22261 ttcatatcaa cctttatttc atacataaaa ctatatttat caaataactt tttatatact
    22321 tctcctccag aatcaatttc atcaatatta atatcaaatt ctcccactgt tgcatatctc
    22381 tctaattgac taatttcttt tgataaatta aaatagcccc attcagcctt actgcaagca
    22441 gttgtaacgg atattaacat taataatgcc agtaataacg aaaaagcttt tttacatttc
    22501 atttattatc ccccgtttca ttaattaatt cttaccatct tataataaca taactattta
    22561 atctaaacaa gaataatatg gaatatttat taaaacttat acaattgctg cagtttttaa
    22621 agcattcagg atttcttctt gcttcttatg attctctatt tcatactggc tccattgttc
    22681 tatgtatttt tctaattgtt cttcttctat ctttataaat ttcagatact cttttaatcc
    22741 tttattttgg gcaatacgtt tcatcgtatc aagatttttt tgataatgct tattctttac
    22801 atcatataga caaataattt ccttatcgct aataatgata tgttcaataa gcgtcattcc
    22861 tatctggtct cctagtaagg cactattcca cacaatataa tcctcaggaa gattcgatat
    22921 gacttctgca atttttttat ccccatcttt aaaatgaatg attgaaaaaa attgaacccc
    22981 atattgagca accactaaag taaaaactgc agcaataact gtaaaaatat tttttcttgt
    23041 ccttgtgatg ataaatccta ttaagaatat catcatcata atcacaaaaa aaatcagggt
    23101 ataagtaaga tattttttct ttcttttctg gatataatct gcttcctgta ttacttttgc
    23161 catcgtattc ttcctttcaa gtcaaaatgc ataaaattta cagagctata ctttagataa
    23221 aactctgttt ttaagtttat caaaaaatca acaataatta aagtgtgcaa aagcacactt
    23281 taattattta aaaacatcaa tggtagaatc aaacaaatct gagttttcct taatgatttc
    23341 tttatttccc attacacaat aaagattttt tgatgtaatt tccttaagca tctgcctata
    23401 ttggatgata tcttcttttt tagtatttaa gatttcatcc cgttcttttt gaatatcttc
    23461 ctgagaaatt tgactaatat ataaagatac cgctttttca cctttcatag aaggtgttaa
    23521 tggaaaatca taacgactga tcgtaccaat aatgtatttt gtcatatccc tttcactggc
    23581 atcaaaattt tgaatataat ctgctgcagc atcatagacc tcaagcgttt tcttaagatt
    23641 agggtcccta taggaagcaa aatagatatt tccagttctt gaaacattaa agaatgctcc
    23701 ataagcgcct ccctgtaccc ttactttatt ccatagataa tctaaactga taatgatttt
    23761 taatacttgc atgctgcccg aatagttata actcaatggc agaaaattat atcccttggc
    23821 cacatattga acatcacttt gcgtcattaa gccttcattt ttatttgtaa tattaaatgt
    23881 ataattttgt tttacccgtt tttctttatt cagattttca gttaatagtg aaagattgtc
    23941 ctgaaatact ttataatctt tttcttcact ggtaagacct atgattaaat gattgatatt
    24001 aaatatagca gaagctgctt tctttaaatt ggatataata tctacttttt tagcctgaaa
    24061 attttcttcc aggtctacaa taaattgata aaaggaaaaa ccttttaact gctcattata
    24121 agcacccttt tcagtgaaat aagacataag tctttcataa gccacaacat gaccttgagc
    24181 ataaagattc atttccatgc gggatttcat ttccctgata atctctaata aacgtttttc
    24241 atcgtcaaat ttagttaaat caatgatttc tttaagcagt ttaataagct gtggaacttt
    24301 ttctgttaat gcttttgatt taataataaa cttactttca tattccttgt ctgatccatt
    24361 aatgccaaaa gtgtctgcgt gaatacttat acccccggta tgaatgttaa tttcattcga
    24421 taaatcgccg taatcatagt tttctgtgct cattttaccc aagataccta cgagcattcc
    24481 cccatatggg attaaatcct gctcaagcgt tttcaaatca aaatatagat ttaaatacgt
    24541 aattttattt gtaaataaag gatgaaagag gactttaatc ccttgttctt ctttttcaac
    24601 ttgtggaagt ttttctgctt cttttttgat atcttctaaa gaaagcaaag gaattttttc
    24661 tatatctttt ggatcatccg gagtcatttg tcttttttga agttcctttg tttttttaat
    24721 taaatcggct atttcttctt tagataattt ggccttataa gcagctaact tttctcttgt
    24781 ttttttcatt ttttcttcta ataaaccttt tttaggactc aaaatgagca aagaagcatg
    24841 ctgattattc aataaataat tttcaattaa tgcttcaaaa taagaagaat taaagctttc
    24901 ttttatcttt gcaaggcttg cttcataaca aagatgcata aagggagcgt catcataaag
    24961 ccagctgctc atacttttta tagcataaat aagtcctttt gggtatcctc ttgaatctgc
    25021 ttctctcaag ctgaattcct ttgcattaac tgcagcctga accagttttt tatcaatacc
    25081 gtcctttacc aattgagcca aagtatcttt tacgatttga acaaattgtt cttttttatc
    25141 tatatcactg ttttttacaa tgatgctaaa aatgggctgt tgaatactat tgtcaaagct
    25201 tccaaaaaca tcttttccta tttcagcctc taataaagct ttcttcagag gagaagcagg
    25261 tgtttctaaa agcaaatatt ccaatatctg aaaggccaga tatatttccg gatttgttga
    25321 acggtctatg acgtaattta aactcaaata tgttttacac tcctggcttt cttccattga
    25381 aatcggatag tctgcctgaa tttctttaat ttcatcaaaa ggtttttgca gttcaattga
    25441 agaatcaatc ttttgtctct caaaatggct taaatattcc cggtcaataa attcaagctg
    25501 ctcctctaag tttccatttc cataaagatg aatataggca ttggaaggat gataaaactt
    25561 cttatgaaaa gcaataaagt cttcataagt taattccggg ataaaatcag ggtcacctcc
    25621 tgactctacg ccgtaagggg tatctggaag caaagattcc tggatttttc taaataaaat
    25681 ggattcagga gaagaaaaag cccctttcat ttcattatat acaacgccct tataagtgat
    25741 ttcatcttcc tcattttcca actcataatg ccaaccttcc tgcatcaata tttcaggata
    25801 tttatagata ttgggataaa aaacagcatc cagatagaca tccattaaat tcataaaatc
    25861 tttatcattc gtgcttgcta taggatacat tgttttatcc gcaaaagtca tagcattaag
    25921 gaaagtattt aatgacccct ttgccaattc cacgaaagga tcttttgtag gaaattttct
    25981 ggaaccgcat aatacagaat gttccataat atgaggcaac cctgtactat catgaggagg
    26041 tgttctgaaa gaaattgaaa aaaccttatt ctcatcatca ttttgcaaac ttaataattt
    26101 tgctccgctc ttctcatgtt caaataaacg ggcaatagaa ttaatttctt taatttcttt
    26161 ttcttcaatt aaaataaagc catgaatttt tgaattaact ttcatcctct gcatctccca
    26221 ctttctaaat tattgtttat aataattata tcttaacgcc atttaaaata ttttaaa
//
LOCUS       DAOSUD010000026        25097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1295_length_25097_cov_26.477717, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000026 DAOSUD010000000
VERSION     DAOSUD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 25097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25097
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1295_length_25097_cov_26.477717"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..351)
                     /gene="flgM"
                     /locus_tag="PLG49_05000"
     CDS             complement(73..351)
                     /gene="flgM"
                     /locus_tag="PLG49_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOQ16713.1"
                     /translation="MKVSRVDKVMEIYSSSKMKKTEKPKRTENKDTVALSEQAKDYQK
                     DLKAVSKVPDVREEKVEEIKERIQSGTYNVSAEELAEKLVDSIFDMKI"
     gene            complement(441..866)
                     /locus_tag="PLG49_05005"
     CDS             complement(441..866)
                     /locus_tag="PLG49_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOQ16714.1"
                     /translation="MEVINCKKCNRLFQYIGGRMICPVCHEEEEQEFKLLKEYLYEHP
                     KATMVEVSQATGVSVTAIRHYLREGRLIITNDSPIGIECEKCGIEIKTGRFCERCAYE
                     LEMGMRMATNELLEKNKNPYANTTKSKMHYLRRTKDDYK"
     gene            complement(893..1879)
                     /locus_tag="PLG49_05010"
     CDS             complement(893..1879)
                     /locus_tag="PLG49_05010"
                     /inference="COORDINATES: protein motif:HMM:NF026948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOQ16715.1"
                     /translation="MIDFLLDLIYPPRCIFCRSIIPVEKERGICENCKEMVSFVKGKQ
                     CQKCGKPIEDRTDKKLCVDCMRMNHYYEKGFALFVYEGLVRDMIYRFKYGGHKEYARH
                     LGKWMADVIIKADIKEKIDLIIPVPIHPNRKKKRGYNQSEELARVLAKELKIPMNASA
                     LIRKKDTIAQSGLTFLQRRNNMKNAFEFDNGFGIYHKNILLVDDIYTTGATINACSKL
                     LKKKSANTVYFWQFPLDVTLVRRNRMSYDICSICGRFFDKSGRNYCDECYEKDKVEYK
                     AVRDYVIQHKGCTVMEVSTITGISVKTIYRYINEGRIEIDEKSHNKLIRPID"
     gene            complement(1900..4140)
                     /locus_tag="PLG49_05015"
     CDS             complement(1900..4140)
                     /locus_tag="PLG49_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOQ16716.1"
                     /translation="MDEPIIIEGVVDDIIFHNEANGYTVCSINFEEDEIVCVGILLES
                     IHPGENIKIIGSWVNHPIYGKQLQIESYEKLMPKTENEIERYLSSGIIKGIGPTLAKR
                     IVKKFGLDTFRIIEEEWERLEEVKGISRQKALQIGELFHEQRELKRIALFLQDYDISP
                     SYALKIYKQYGEATIDIIKNNPYQLAEDILGIGFKMADKIGRTMGIDEASPHRIKAGI
                     KYILNLYGNNGHTYVPRDKLLNTACELLEIEKEFIENGLLELQILKEIHQEKLGDMEA
                     VYLSAFYFKEINVARRLIDLSYTRKEVDIAEIEGRIDKLENEKGIQLAKEQKEAIKQA
                     MCEGLLIITGGPGTGKTTTINIIIQLLEEEGMEVLLAAPTGRAAKRMTEATGKEAATI
                     HRLLEISFIEEDNKKQVFERNEERPLEADVVIIDEMSMVDILLMDSLLKAIAPGTRLI
                     CVGDADQLPSVGPGNVLKDMIRSQTIKVVHLTEIFRQAEESAIIMNAHKINQGEYPLL
                     NEKEKDFFFIKRSVQDEVIETIKELIIKRISSFKNCNPLKDIQVLTPMRKGPLGAINL
                     NVILQKALNPPSKKKKEKSYGTNTFREGDKIMQIRNNYNLPWKIYNERGIIIDEGVGI
                     FNGDGGIITAIDEEAQTLTVLFDDMKTVEYDFSQLDELELAYAVTIHKSQGSEYPIVI
                     IPIHSGPPMLMNRNLLYTAVTRAKELVVIVGLTSMLYRMVDNNREIDRYSSLSYRLEK
                     IKNLSL"
     gene            complement(4237..5418)
                     /gene="metK"
                     /locus_tag="PLG49_05020"
     CDS             complement(4237..5418)
                     /gene="metK"
                     /locus_tag="PLG49_05020"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOQ16717.1"
                     /translation="MSRKLFTSESVTEGHPDKICDQISDSILDAILEQDPYGRVACET
                     AVTTGMVLVMGEITTNCYVDIPKIVREKIKEIGYNHAKYGFDGDTCAVITSLDEQSPD
                     IAMGVNKSYEARQKGEEDIDAIGAGDQGIMFGFACNETPELMPLPISLAHQLSRRLAY
                     VRKNNILPYLGPDGKTQVTIEYEDDKPLRVHTVVISSQHDAAVDQETIKKDIIEHVIK
                     PVIPKELLDDDTRYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYVPHGGGAFSGKD
                     PTKVDRSAAYAARYVAKNIVAAGIAEKCEVNLAYAIGVAQPVSILVNTYGTGKISDEA
                     ITELIKKHFDLRPAAIIRDLDLRRPIYAKTAAYGHFGRTDIELPWEKTDKAEQLRKEV
                     L"
     regulatory      complement(5514..5621)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5644..5997)
                     /locus_tag="PLG49_05025"
     CDS             complement(5644..5997)
                     /locus_tag="PLG49_05025"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOQ16718.1"
                     /translation="MKINSLNNNLTTFSIDNALIQKESQKAENFQDVLKKAKEEKDDE
                     SLKKACKEFEAYFISRLFKEMRSTIMPGGLIEKSRGEEIFEEMLDDEYSKLAANGRGM
                     GLADMLYKQLSKDIK"
     gene            complement(6014..6829)
                     /gene="flgG"
                     /locus_tag="PLG49_05030"
     CDS             complement(6014..6829)
                     /gene="flgG"
                     /locus_tag="PLG49_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOQ16719.1"
                     /translation="MMRSLWTAATGMKSQQLNVDNIANNLANVNTVGYKKSRVEFKSL
                     LYETMARAGIDEEGAGSPVSLQIGHGVRPIATTKDFTMGNIEMTNNPLDLAINGEGFF
                     AVLTPDEEVLYTRDGSFKLSVVDDELMLVTSDGYSVLDINDEPIYIPPEVSVSSLTIA
                     EDGRLYYVDNENNTVDLDLQIQLVQFRNRAGLEAVGQNLFRQTPASGEPMYEVDGEVN
                     QSSKIIQGSLETSNVQVVEEMVKLIVAQRAYEINSKAIQTSDDMLAQANQLKR"
     gene            complement(6842..7603)
                     /gene="flgF"
                     /locus_tag="PLG49_05035"
     CDS             complement(6842..7603)
                     /gene="flgF"
                     /locus_tag="PLG49_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOQ16720.1"
                     /translation="MVRGLYTSAIGMKTQFKRMDVISNNLANVDNTGFKKDIVVSRSF
                     PEELTKRINDRQNGFPNNRNIGRMSLGLYIDEVYTNFAQGSLSLTNDPFNLALDGKGF
                     FAIQAEDGTERYTRDGSFVLNSDGILMTKEGNYVLGENGIISLEEQGNIRIDEEGNIY
                     LNNELVDRLRIVNFNNPESLRKVGDNLLERSNQSAEIAFEGKVLQGYLETSNVNVVQE
                     MVDMITATRIYEANQKAVQTHDETLGKAVNEVGRV"
     gene            complement(7621..8607)
                     /gene="mreB"
                     /locus_tag="PLG49_05040"
     CDS             complement(7621..8607)
                     /gene="mreB"
                     /locus_tag="PLG49_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HOQ16721.1"
                     /translation="MDMGIDLGTASVLVYIRGQGIALKEPSVVAIDNNTNKILAVGEE
                     ARKMLGRTPGNIVATRPLKQGVISDYEVTEMMLKYFIRKAVGKKLTRRPRIAVCVPSG
                     VTEVERKAVEDATKEAGARQVYIIEEPIAAAIGAGIDIGRACGSMVVDIGGGTTDIAV
                     ISLGEPVVSSSLKIAGDNFDEAIIRYMRKKHNVLIGERTAEDLKVNVGTAFKRPKPVS
                     MEVRGRNLITGLPKTISVSSDEMLEALQEPVSNIVEAVHAVLEKTPPELAADISDRGI
                     VMTGGGSLLYGLDKLIKEKTGINVIIAEDAISSVAIGTGKYVEYIASQKNFR"
     gene            complement(8803..9081)
                     /gene="spoIIID"
                     /locus_tag="PLG49_05045"
     CDS             complement(8803..9081)
                     /gene="spoIIID"
                     /locus_tag="PLG49_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOQ16722.1"
                     /translation="MKGYIEERAIEVAKFIIGSNATVRETAKKFGISKSTVHKDVTER
                     LEKINPKLALEARKVLEINKAERHLRGGMATREKYLSMASKKKSKKQG"
     gene            complement(9165..10046)
                     /locus_tag="PLG49_05050"
     CDS             complement(9165..10046)
                     /locus_tag="PLG49_05050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ16723.1"
                     /translation="MKSKKFWTYFNKKGYYGMLLLCLLTIIVTTMVTNNNLNTLEKVR
                     EENYIDLDEEYGTFDLEDVGTINAYQNDLTITEEDIAGSGAAVAEESSPGRTDEAEGE
                     NNETAKEEEVTVALETQANNNPAPLTVVEKSDEEPVFIGTEEANGETVETFSFSEDTI
                     IQWPSYGEIVMDFSADKLVYDKTLEEYRVHPAVCIAAADNEEVRAAAKGRVEVIKKEP
                     ETGVTVVINHGDGWKTIYGQLQEELAIRQGEVVEKGQLIGKIAEPTKYSALLGKHVYF
                     KIEKGGKPVDPKQFQHE"
     gene            complement(10089..11018)
                     /gene="spoIID"
                     /locus_tag="PLG49_05055"
     CDS             complement(10089..11018)
                     /gene="spoIID"
                     /locus_tag="PLG49_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOQ16724.1"
                     /translation="MKGIKRGGAITSEKESIEDNNILTIKVLNEESQEIMEMDFEEYI
                     KGVVAAEMPASFELEALKAQAVAARTYAIRKMKQNKNKEGADISTDSFKGQAYLSLEQ
                     LKEKWGDNFYAYYNKISQAVEETKGEIMLYEEEPIEAVFHSTSAGITQSARDIWQVDV
                     PYLQSVESVGEEESPAFMEEKRILKKEVVESLKRKYPDIMIQDLIGQIQIIERTEGGY
                     IKKIQIGNKIFSGEEVRYLLNLKSSCFTIKEDGDTLIFVTKGYGHGAGMSQYGANYMA
                     KEGKVYTDILKHYYQGVEIKSLEDIEGNLFKNE"
     gene            complement(11222..12475)
                     /gene="murA"
                     /locus_tag="PLG49_05060"
     CDS             complement(11222..12475)
                     /gene="murA"
                     /locus_tag="PLG49_05060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ16725.1"
                     /translation="MRLIVRKSPPLRGTVRISGSKNSVLPILAASLLAEGKSKIGEVP
                     FLKDVVIMQDLLKYFGAEVKWDKVNGFIEICADNINGYEAAYDLASQMRASFLVMGPL
                     LARIGKAKVPLPGGCAIGSRPVDLHLKGFAALGADIKQQNGYVEAYANKLKGAKIYLD
                     FPSVGATENIIMAAVYAEGTTIIENAAVEPEIVDLANYLNGMGADIRGAGTDSIKING
                     VDKLKGTSHNVIPDRIEAGTFMVAGAITRGEIFLENVVSDHLKPVIAKLKECNVEVTE
                     SEKGIKVCGKDVIKAVDIKTLPYPGFPTDMQAPFMSLLSIAEGTSMVIETVFENRFMH
                     VGELKRMGAQIKIESRSAIIEGVKKLTGTQVRATDLRAGASLILSALVAEETTEISDI
                     YHIERGYSEIDKKLIALGADLEKVK"
     gene            complement(12493..13239)
                     /locus_tag="PLG49_05065"
     CDS             complement(12493..13239)
                     /locus_tag="PLG49_05065"
                     /inference="COORDINATES: protein motif:HMM:NF020264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HOQ16726.1"
                     /translation="MKRNYSIFLAVLLILYSVSLFERGNTKFEEETLVTTFNNSQFQP
                     IQINLNTWGTLNNSFSTLEEMRELSKHFTDDLKISNFDHVEEKESESLREFTVIRASK
                     NAKTTVKLQSIKDELSDTANTYLVIDIIIYDKYKGLAYLKEKLDKIYAANDIKANTNI
                     TIMGHMKGKLTEKERDKICKSIMKNIDARVEEVYETEKIYSVYGYTKLIDEYITSNQK
                     KINVNCAFRYNEYENKTYLYLATPLITVEY"
     gene            complement(13959..14369)
                     /locus_tag="PLG49_05070"
     CDS             complement(13959..14369)
                     /locus_tag="PLG49_05070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOQ16727.1"
                     /translation="MAEKKTLLKIITPDRNFFDEEVDVVIFRTIQGDMGVLPGHAPLT
                     TILSIGIIRIKQGEEEKRATLIGGFAEIQPDRITILADAAEWPEEIDKERAEAAKRRA
                     EERLTKKTEDINVARAEAALKRALVRLELCQYRE"
     gene            complement(14389..15789)
                     /gene="atpD"
                     /locus_tag="PLG49_05075"
     CDS             complement(14389..15789)
                     /gene="atpD"
                     /locus_tag="PLG49_05075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOQ16728.1"
                     /translation="MADANIGKIVQIIGPVLDIKFSPEHLPALNNAIIIEKENGETLT
                     AEVAQHLGDDIVRCIAMSSTDGLMRGMEAKDTGDCISVPVGEKTLGRIFNVLGQPIDN
                     KPAPEVEEKWPIHRPAPEFTEQSTDIEIFETGIKVVDLLCPYSKGGKIGLFGGAGVGK
                     TVLIQELIRNIATEHGGYSVFTGVGERTREGNDLYYEMANSGVLDKTTMVFGQMNEPP
                     GARMRAGLTGLTMAEYFRDRTGKDVLLFIDNIFRFIQAGSEVSALLGRMPSAVGYQPT
                     LATEVGALQERITSTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRDIVEL
                     GIYPAVDPLVSTSRMLDPAIVGEEHYKVARGVQEVLQRYKELQDIIAMLGMDELSEED
                     KLIVARARRIQRFLSQPFFVAENFTGLPGKYVPISETIRGFKEILEGKHDDLPEAAFH
                     MVGTIDDAVEKAKGLQ"
     gene            complement(15818..16684)
                     /gene="atpG"
                     /locus_tag="PLG49_05080"
     CDS             complement(15818..16684)
                     /gene="atpG"
                     /locus_tag="PLG49_05080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOQ16729.1"
                     /translation="MASMREIKRRIRSVNSTGQITKAMKLVSTVKMQKARKDLESTRP
                     FFNIVQKTISSIVNSSKGISHPYLEQREVKKTLYIVMASDRGLAGGYNNNVCKLALKY
                     MEERNKADIELITVGRKAKDFFERRGYKINKAYIKISEEPKYSNAKEIEENIMQLYSE
                     GKVDEVYLVYTSFKSTIDQKPMVKKLLPVDTSDFQEESQEQRRTLMIYEPSPESVLEY
                     IIPKYIASVIYGAMFEAAASEQGARMTAMDSATKNAEEIIDNLTLHFNRARQAAITQE
                     ITEVVSGAEALK"
     gene            complement(16697..18211)
                     /gene="atpA"
                     /locus_tag="PLG49_05085"
     CDS             complement(16697..18211)
                     /gene="atpA"
                     /locus_tag="PLG49_05085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOQ16730.1"
                     /translation="MNLRPEEISSVIKKQIQNFQTKLEVSNVGTVLQVGDGIVRIHGL
                     EKAIAGELLEFPGGVYGMALNLEEDNIGAVLLGSDKGIKEGDIVKSTGRVVEVPVGDA
                     LIGRVVNALGQPIDGKGPIHTTKYRPVERIAPGVIERKSVDTPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQDVLCIYVAIGQKASTVAQLVKVLEDHGAM
                     DYTTVVSATASELAPLQYIAPYAGCAMGEEWMEEGKDVLIIYDDLSKHAVAYRAMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDERGGGSLTALPIIETQDGDVSAYIPTNV
                     ISITDGQIYLETELFNAGIRPAINPGLSVSRVGGAAQIAAMKKTAGPIRVELAQYRDL
                     ESFAQFGSELDKATRDALAQGERIIEVLKQPQYKPMSVEHQILILFAVTRKYLMDIDV
                     ADIRRFEEEFLEFMDTKYLDIVDTIRTEKKIDSDLENKIDGAIKEFKKIFKNEM"
     gene            complement(18228..18773)
                     /locus_tag="PLG49_05090"
     CDS             complement(18228..18773)
                     /locus_tag="PLG49_05090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOQ16731.1"
                     /translation="MGQLISDRYAKALFDLAVESNKIDEFESQVGSLYTILKTEKDFT
                     ELLQHPQIIAEEKIELLQKVFEAKVSSEIMGLLVLIIRKNREEYLLDVLNAFLDKVKE
                     YKGIVTATVISAVALDNEQISQIKMLLTKRLKKQIQIETQVDHSIIGGLIIRVGDSIL
                     DASIGGRLQALKASLYDSQLV"
     gene            complement(18761..19279)
                     /gene="atpF"
                     /locus_tag="PLG49_05095"
     CDS             complement(18761..19279)
                     /gene="atpF"
                     /locus_tag="PLG49_05095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOQ16732.1"
                     /translation="MDGSLLSFDKQFLFSLAVQLINTLILFFFLGKLLFKPLTEFMDK
                     RSEKIKGQIDFARAEEEKALKLKEEYEAKLQKIQEEADAIIKEAREKALKKENSIIEE
                     ARKESEMIRKRALAEIEREKAKAQDEMKKEIIEIASLIAEKFIAASIDEEKQKALIDE
                     AISKMGDVSWDS"
     gene            complement(19365..19649)
                     /gene="atpE"
                     /locus_tag="PLG49_05100"
     CDS             complement(19365..19649)
                     /gene="atpE"
                     /locus_tag="PLG49_05100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOQ16733.1"
                     /translation="MEGIDGRALILACSAIGAGLALIAGIGPGIGQGYAAGKAAEAVA
                     RQPEAQNEIIRTLLLGDAVAETTGIYGLFIAIILVFANPLINKYLSLLGQ"
     gene            complement(19737..20429)
                     /gene="atpB"
                     /locus_tag="PLG49_05105"
     CDS             complement(19737..20429)
                     /gene="atpB"
                     /locus_tag="PLG49_05105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOQ16734.1"
                     /translation="MEGLDFSNKYVFPVHIGGEEFYITQSIISTWIVMAALIIFALVV
                     RFSISRFKQVPKGFQNVVEIIGEVVNTFTISTMGERNKRFAAFYGSVFLFLLFSNLWG
                     LLGMRPPTSDLATTFGLALMTFVMVQYYGIKTGGIKNYIKGFFEPLPFLLPLNVIGEL
                     ATPVSLSFRLFGNIVAGVIIMGLIYAALPGLTKIGVPAVLHVYFDVFSGALQAFIFVM
                     LSMIFVSNAIQE"
     gene            complement(20483..20851)
                     /locus_tag="PLG49_05110"
     CDS             complement(20483..20851)
                     /locus_tag="PLG49_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOQ16735.1"
                     /translation="MKRLIRQIIAISFFIGISGLFLVNEPFAWIKGLFFGTIISILRL
                     FLIEATIKKAIKMSPAKASRYASSQYFIRYFLTAIVLIVAALEPSINLIATIIGLFTI
                     KFSVYLTLITDKKGSNNSKH"
     gene            complement(20851..21090)
                     /locus_tag="PLG49_05115"
     CDS             complement(20851..21090)
                     /locus_tag="PLG49_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOQ16736.1"
                     /translation="MKKKFDFLTALSLISQIGFMMAVPIIFGVIFGNYIDNKLNTGIV
                     FLLIFSLLGIGAAFRNLYILTTKALKNGDKRKDQK"
     gene            complement(21188..22339)
                     /gene="wecB"
                     /locus_tag="PLG49_05120"
     CDS             complement(21188..22339)
                     /gene="wecB"
                     /locus_tag="PLG49_05120"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOQ16737.1"
                     /translation="MSIKVMSIFGTRPEAIKMGPVVKELENTPGIDSIVCVTAQHREM
                     LDQVLEVFNIVPDYDLDIMKERQSLKDITIHALEGLDKVIKEVVPDIVLVHGDTSTTF
                     VGALAAFYNQIKVGHVEAGLRTYDKYQPFPEEMNRKLTGSLTDLHFAPTPLAKEHLLK
                     EAISEEQIFITGNTVIDALKTTIEDNYVFSLPLLNEIDYKNKKVITMTAHRRENLGKP
                     LENICKGVYRLVEAFDDVEVVYAVHKNPAVREIVYPILGNHSRIHLIDPLDLKDMHNL
                     MNLSYLVLTDSGGLQEEVPSMKKPVLVLRNVTERPEGIKAGTLKLAGTDEDRVFNMAK
                     ELLENKEEYEKMTKAKNPFGDGNASRRIVDAILYSFGKIVERPKDFIIF"
     gene            complement(22531..23589)
                     /locus_tag="PLG49_05125"
     CDS             complement(22531..23589)
                     /locus_tag="PLG49_05125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ16738.1"
                     /translation="MKLFQGIIMQDKFLIYFIAFGMAFLISLLTTPLSKMIAFKVGAV
                     DQPKARGMHDKPMPRMGGIAIFLGFIFTVLVVTPSVEAFEWKQISGLLVGGMIIFLLG
                     FFDDIYNLNAKLKFLIQVIAALVVIYSDIRIEFVTWPFAEGNILILEKISIPITLFWI
                     VGVTNAVNLIDGLDGLAAGVSSIAAICLMILSMFSPNPIGPVAAILMAALAGSCLGFL
                     PYNFNPAKIFMGDTGSTFLGFVLAVSSIQGLIKGYTAVTILIAVLVLALPIFDTTFAI
                     LRRIISRRPIMEADRGHIHHRLVDKGYSQKQAVITLYGISGGFGIIGILLALKDTHMA
                     LGVFIAMGIALYMITRNK"
     gene            complement(23941..24384)
                     /locus_tag="PLG49_05130"
     CDS             complement(23941..24384)
                     /locus_tag="PLG49_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOQ16739.1"
                     /translation="MRPSWDEYFMEIVELIKERSTCLRRQVGALIVNDKRILATGYNG
                     APTGCSHCEEVGCLREKLQIPSGQRHELCRALHAEQNAIVQAALYGISVQGGTLYVTH
                     QPCVMCAKMAINAGIKRIVYKGEYPDELSRELLEEAGIEVVNFKG"
     gene            complement(24398..25027)
                     /gene="upp"
                     /locus_tag="PLG49_05135"
     CDS             complement(24398..25027)
                     /gene="upp"
                     /locus_tag="PLG49_05135"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOQ16740.1"
                     /translation="MGQLHVMEHPLIQHKISMLRDKNTGSKEFRELVEEISMLMCYEA
                     TRDLPLEEVEVETPVAKAKAKVLSGKKLGIVPILRAGLGMVDGMLNLIPAAKVGHIGL
                     YRDPETLQPVEYYCKLPYDSPTRDIFVLDPMLATGGSASAAIQFIKDKGVQRIRFLCL
                     IAAPEGVERLMKDHPDVDIYVAVLDERLNDHGYIVPGLGDAGDRLFGTK"
BASE COUNT         7408 a         5453 c         3409 g         8827 t
ORIGIN      
        1 cttcatattc aattatttaa taattataaa aaaaaattaa aaagcaatcg tagatgaact
       61 ttttataatg atttatatct tcatatcaaa aatactgtct actaactttt ctgctaattc
      121 ttcagcactg acattataag ttccagactg aattctttct ttaatttctt caactttttc
      181 ttcccttaca tctggtacct tggaaacagc tttaagatcc ttttgatagt cttttgcctg
      241 ttcagataag gcaaccgtat ctttattttc tgttcgcttt ggtttttctg tttttttcat
      301 tttagaacta ctatagattt ccattacttt atcgactctt gatactttca taatatcacc
      361 tgccttcctt ataccatcca tctttacttc tctacttatt atatcgtcat aaaaaaattg
      421 agaatttaga cctatatcaa ttatttataa tcgtctttcg ttcttcttag ataatgcatc
      481 ttggatttcg tcgtgtttgc atatggattt ttattttttt ctaaaagttc attggttgcc
      541 attctcatac ccatttctaa ttcataggca catctttcac aaaatcttcc tgtctttatt
      601 tcgataccac atttttcaca ctcaatacct ataggagaat cattggtaat aatcagtctt
      661 ccctccctga gataatgcct gatggctgta acagatactc ctgttgcctg ggatacttct
      721 accattgttg ctttgggatg ctcatataaa tattctttta ataatttaaa ttcttgttct
      781 tcttcttcat gacaaaccgg acaaatcatt ctgccaccaa tatattgaaa aagcctgtta
      841 catttcttgc aattaataac ttccattttt catcccccat ttcttaaaaa ttttaatcta
      901 tcggtctaat caacttgtta tgtgattttt catcaatctc aattcttccc tcattaatgt
      961 agcggtatat ggttttaacc gaaatgcctg ttatagtgga tacctccatt accgtgcagc
     1021 ctttgtgttg aatcacataa tcccgtacag ctttatactc aactttatct ttttcataac
     1081 attcatcaca ataatttctg ccgcttttat caaaaaatct gccgcaaata ctgcaaatat
     1141 catatgacat cctattcctc ctaactaaag tcacgtccaa tggaaactgc caaaaatata
     1201 cggtatttgc acttttcttt tttaacaatt ttgaacaggc attaatagta gcccctgtag
     1261 tatatatatc gtcaactaat aatatatttt tatggtaaat accaaaacca ttatcgaatt
     1321 caaaagcatt cttcatatta ttccttcttt gcaaaaaggt caggccactt tgtgctatgg
     1381 tatctttttt tctgattaaa gctgaggcat tcataggaat ctttaattct ttagcaagca
     1441 cccttgctaa ttcttcactt tgattatagc ctcttttctt ttttctgtta ggatgaatag
     1501 gaacaggtat aattaaatct attttttctt ttatgtctgc ttttataatt acatcagcca
     1561 tccattttcc aagatgcctt gcatattctt tatgcccccc atatttaaat cggtaaatca
     1621 tgtctcttac taaaccctcg tagacaaaaa gggcaaatcc tttttcataa taatgattca
     1681 tcctcataca gtcaacacac agctttttat ctgtcctgtc ttctatgggc ttgccacact
     1741 tttgacactg cttcccttta acaaaagaaa ccatctcttt acagttttca caaatgcctc
     1801 tttccttttc aacaggaata atactcctgc aaaaaataca gcggggagga tatatgagat
     1861 ccagtaaaaa atcaatcacc ttttttcacc ttcataaatt tacaaagata aattttttat
     1921 tttctctaac ctgtaagaaa gactagaata tctgtcaatt tcacggttat tatccaccat
     1981 ccggtaaagc atactggtca gtcctacaat aacaaccaac tctttagctc tagtaactgc
     2041 tgtatataat aaattcctgt tcataagcat aggcggtcca ctgtgaatgg ggatgatgac
     2101 aataggatat tccgaaccct gggatttatg gattgtaaca gcataagcca attccaattc
     2161 atctaattgg ctgaaatcat attctacagt tttcatgtca tcaaaaagaa ccgttaaagt
     2221 ttgggcttcc tcgtctattg ctgtaattat cccaccatcg ccgttaaaaa ttcctacgcc
     2281 ttcatctatt ataatccctc gttcgttata gattttccat gggagattat aattattcct
     2341 gatctgcata attttatccc cttcacgaaa agtattcgta ccataacttt tttctttttt
     2401 cttctttgaa ggaggattta aagccttttg aagaatgaca ttaagattaa tagccccaag
     2461 aggtcctttt ctcatagggg ttaaaacttg aatatccttt aggggattgc agttcttaaa
     2521 agaagaaatc cgttttataa tcaattcttt aatggtttct atcacttcat cctgtacact
     2581 tctttttata aagaaaaaat ctttttcttt ttcattgagc agagggtatt ctccctgatt
     2641 gattttatgg gcgttcatga taatagcact ttcttctgcc tgacgaaata tttctgttaa
     2701 atggacaact tttatggttt gactgcgaat catatccttt aaaacattac ctggacctac
     2761 agaaggcagc tgatccgcgt cccctacaca gattaatctg gttcctggtg caattgcttt
     2821 aagcagactg tccataagta agatatctac catagacatt tcatcgataa taaccacatc
     2881 ggcttcaagg ggtctttctt catttctttc aaaaacctgc tttttattat cttcctcgat
     2941 aaaacttatt tctaacagcc tgtgaatggt agcagcttct tttcctgttg cttctgtcat
     3001 ccgttttgct gctcttcctg tgggtgctgc caaaagaact tccatgcctt cttcttcgag
     3061 caattgtata ataatattga tagttgttgt ttttcctgtt cccggaccac ccgtaatgat
     3121 aagaagacct tcacacatgg cctgttttat agcttctttt tgttcctttg ccagttgaat
     3181 ccctttttca ttttctaatt tgtcaatcct tccttctatt tccgcgatgt caacttcttt
     3241 cctggtataa gaaaggtcaa taagtcttct ggctacattg atttctttaa aataaaatgc
     3301 tgacaaataa accgcttcca tatccccaag cttttcctga tgaatttctt taagaatttg
     3361 gagttctaaa agtccatttt caatgaattc tttttctatc tccagtaact cacaagcagt
     3421 atttagtaat ttgtccctgg gaacataggt gtgaccatta tttccatata agttgaggat
     3481 atatttaatt cctgccttta ttctgtgagg agacgcctca tcgatgccca ttgtcctgcc
     3541 tattttgtct gccattttaa accctatccc caatatatct tctgcaagct gataaggatt
     3601 gttttttata atatcgatag ttgcttcccc atattgctta taaattttaa gggcatatga
     3661 aggagatata tcataatcct ggagaaataa ggctattctt tttaattccc tttgctcatg
     3721 aaaaagttct ccaatttgca gggctttttg tctgctgatt ccttttactt cttccaaacg
     3781 ctcccattct tcttctataa ttctgaacgt atctaaacca aactttttga caattcgctt
     3841 ggcaagcgta ggacctatcc ctttaataat ccctgaactt aaatatcttt ctatttcatt
     3901 ctctgtttta ggcattaatt tttcatagct ttcaatttgt agttgtttgc cgtaaatggg
     3961 atgattgacc caggaaccta tgatttttat attttcacca ggatggatgc tttctaataa
     4021 aatccctaca cacacaattt catcttcttc aaaattgatg gaacataccg tgtatccatt
     4081 agcttcatta tgaaaaataa tatcatcaac aacaccttct ataataattg gctcatccat
     4141 atctttcaca ccattcatca tgatattggg caattttatc ataaggtata ttttagaaat
     4201 tgaaaagggc aaaaaagaag ttttacaggt ttgaacctat aaaacttctt ttcttaattg
     4261 ctcagcttta tctgtctttt cccaaggcaa ttctatgtct gttcttccaa aatgaccata
     4321 ggctgctgtt tttgcataaa tcggtcttct aagatctaaa tccctgatta tcgctgctgg
     4381 tctaagatca aaatgctttt taattaactc cgtaatagct tcgtcagata tttttcctgt
     4441 tccgtaagta ttcactaaaa tagatacagg ctgtgcaaca ccaatggcat aagccagatt
     4501 gacttcacat ttttcagcaa tacctgctgc aacaatattt tttgccacat aacgggccgc
     4561 ataggctgca gaacggtcta ctttagtagg gtctttaccg gaaaaagcac ctcccccatg
     4621 aggtacatag cctccataag tgtctacaat gatttttctt cctgtaagac ctgaatcccc
     4681 ctgaggccca cctatcacaa agcgtcctgt cggattaata aaataccttg tatcatcatc
     4741 taataattct tttggtataa caggcttaat cacatgctct ataatatctt ttttaatggt
     4801 ttcctgatca actgcagcat catgttgtga tgaaatcaca accgtatgaa cccttaaagg
     4861 cttgtcatct tcatattcta tggtcacttg tgttttccca tctggtccta aatagggaag
     4921 tatgttgttt ttacgaacat aagccagcct tcgtgataat tgatgggcta aagaaatagg
     4981 caatggcata agctcaggcg tttcattgca ggcaaagcca aacataatgc cctgatctcc
     5041 tgcccctatt gcatcaatgt cttcttctcc tttttgcctt gcttcataag acttattaac
     5101 acccatggct atatctggcg actgttcatc caaagaagtt atcactgcac aagtatcgcc
     5161 gtcaaaacca tactttgcat ggttataacc aatttctttt attttttccc tcacgatttt
     5221 aggaatatcc acataacaat tggttgttat ttcacccatt actaaaacca ttcctgttgt
     5281 tacagccgtt tcacaagcca ctcttccata agggtcttgc tctaatatag catctaatat
     5341 ggaatctgat atttggtcgc aaatcttatc tggatgcccc tctgtaacag attctgaggt
     5401 aaacaatttt ctggacatat atgtcaactc cttacatttt atctttcgat atatattttt
     5461 atacatttat aaaaaagtat taaaaaaacc tcaaaatgag gttttttcta tcttcctcat
     5521 cttccggaat gactccgggg gaattagcac cactgcatag ataacttatg ctggttgccg
     5581 ggtttcatcg ggccctttcc ctccaccact cttgataaga aaatatattg ttttaatctt
     5641 atcttatttt atgtcttttg acaactgctt ataaagcata tcagccagtc ccatacctct
     5701 tccatttgcc gcaagcttgg agtattcatc atccaacatt tcttcaaata tttcttcgcc
     5761 tctgcttttt tcaataagtc ctccaggcat aatggtactt ctcatttctt taaaaagccg
     5821 actaataaaa tatgcttcaa attccttgca ggcttttttc agggattcat catctttttc
     5881 ttctttagcc ttttttaaaa catcctgaaa attttcagcc ttttgacttt ccttctgaat
     5941 aagggcattg tctatagaaa aagttgtaag attattattc agagaattaa ttttcatcga
     6001 ttaatccaac cccttatcgt tttaattgat ttgcctgcgc aagcatatca tctgaagttt
     6061 gaatggcctt tgaattaatt tcatatgctc tttgagcaac aatcagttta accatttctt
     6121 ctacaacctg gacattggaa gtctccagac taccttgtat aatcttactg ctttgattca
     6181 cttctccgtc cacttcatac atcggctctc ctgatgctgg cgtttgtctg aaaagatttt
     6241 gaccaactgc ttcaagtcct gccctgtttc taaattgaac cagttgtatt tgaagatcta
     6301 aatctacggt attattttca ttatctacat aatagagcct tccgtcttct gcaatggtca
     6361 agctggaaac actaacttct ggtggtatat aaattggctc atcattaatg tctaatacag
     6421 aataaccatc tgatgtaaca agcattaatt catcatccac aacacttaat ttaaaactgc
     6481 cgtctcttgt ataaagaact tcttcatcag gtgttaatac agcaaagaaa ccttctccat
     6541 taatcgcaag atccaaagga ttattggtca tttcaatatt tcccatagta aaatctttag
     6601 tagtagcaat aggccttacc ccatgaccaa tttgaagact tactggggaa cctgcacctt
     6661 cttcatcaat gcctgctctt gccattgttt catataaaag agatttaaat tcaacccggc
     6721 ttttcttata acctacggta ttcacatttg ctaaattgtt agcaatatta tccacattta
     6781 attgttgaga tttcattcct gtcgctgctg tccacaaaga acgcatcatc atttatcccc
     6841 cctatactct tcctacttcg ttaaccgctt tgcctaaagt ttcatcatgg gtttgaacgg
     6901 ccttttgatt tgcctcatat attcttgttg ccgtaatcat atcaaccatt tcctgaacga
     6961 cattaacatt agaagtttcc aaataccctt gtagaacttt accttcaaaa gcaatttctg
     7021 cggactgatt gcttctttcc aataaattat ctccaacttt tctaaggctt tcaggattat
     7081 taaaattaac aattctaagc ctgtccacta attcattatt aaggtagatg ttgccttctt
     7141 catcaatacg tatattccct tgttcttcta agctgatgat tccattttca cctaaaacat
     7201 aattaccttc tttggtcatc agtatgccat cggaatttaa tacaaaggaa ccatctcttg
     7261 tatatctttc tgtgccatct tcagcctgaa ttgcaaaaaa tcctttacca tccaaagcaa
     7321 gattgaaagg atcattggtc agagaaagag aaccttgtgc aaaattggtg tatacttcat
     7381 caatatacaa gcccaagctc attctgccaa tattccggtt attgggaaat ccattttgcc
     7441 ggtcattaat ccttttagtc aattcttccg gaaaggaacg agacactaca atatctttct
     7501 taaatccggt attatccaca ttggccagat tattagatat cacatccatc cttttaaatt
     7561 gtgttttcat tccaattgcc gaagtataca accctcttac caaattaaat cccccttacg
     7621 tcatctaaaa ttcttctggc tggcaatata ttcgacgtac tttcctgttc caatggcaac
     7681 actggaaata gcatcttctg caataataac attaatgcct gttttttctt taatcaactt
     7741 atcaagacca taaagcaggc taccgcctcc agtcattaca atacctctgt cagaaatatc
     7801 ggcagccaat tccggaggtg ttttttccaa tacagcatga acagcttcta caatattaga
     7861 aacaggttct tgtaaagcct ccagcatttc atcggaagaa acagaaattg tcttaggaag
     7921 tcctgtaatt aagttccttc ctcttacttc catagaaacc ggctttggtc ttttgaaagc
     7981 tgtaccaaca tttactttaa gatcttctgc agttctttca ccaatcaata cattatgctt
     8041 ttttctcata tatctgatga tggcttcatc aaaattatcc cctgctatct ttaaagaaga
     8101 acttactacg ggttctccca aagaaattac tgctatatct gttgtaccgc ctccaatatc
     8161 tacaaccata ctgccacaag cccgtccaat gtcaattcca gctccaatag cagcagcaat
     8221 aggttcttca ataatatata cctgccttgc gcctgcttct ttcgtagcat cttcaacggc
     8281 ttttctttct acttccgtaa caccacttgg aacgcatacg gcaatacggg gtctgcgagt
     8341 caactttttc cctacagctt tacggataaa gtattttaac atcatttcag tgacttcata
     8401 gtcagaaata acaccttgtt taagaggtct ggttgctaca atattaccag gcgtacgccc
     8461 taacattttt cttgcttctt caccaaccgc taaaatctta tttgtattat tatcaatcgc
     8521 tacaactgaa ggttctttta atgcaatacc ttgtcctctt atatagacaa gcacactcgc
     8581 agttcccaga tcgattccca tatccatgaa cccattctcc tttgttaatc tttcttatat
     8641 atcgacatat ttttattatt aattaataga tatcccttcc cattttatgg tttatacatc
     8701 tattattcat aattatatcg aatatatata aaaatttaaa ggattttgct ataaaaattt
     8761 gtaaaatttt tgtctaacaa caaaaacgtg cttaaagcac gtttagcctt gttttttgct
     8821 cttttttttg gatgccatac ttaaatactt ttctcttgtt gccatgccac cccgaagatg
     8881 tctttccgct ttattaattt ccagaacctt tcttgcttct aaagctaact tagggttaat
     8941 tttttccaac ctctccgtga catctttatg aacggtactt ttactaatcc caaacttttt
     9001 ggcggtttct cttacagtag catttgaacc aataatgaat tttgccactt ctatagctct
     9061 ttcttcaata taacccttca aaagatggcc ccccttaata caactcttga ttaatatata
     9121 tgttaagggg ggatagttta tgatttccta tttagtattt agtcttattc atgctgaaat
     9181 tgctttggat caacaggctt cccgcctttt tctattttaa aataaacatg ttttcctaac
     9241 aaagccgaat attttgtagg ctctgcaatt ttaccaatga gctgaccctt ttcaacgact
     9301 tctccctgcc tgatggccaa ttcttcctgc aactgcccat atatcgtttt ccatccatct
     9361 ccatgattaa tcactacggt tacacctgtt tcaggttctt ttttgattac ttcaacccgg
     9421 ccttttgccg cggctcttac ttcttcgttg tctgctgcag caatacaaac tgcaggatgt
     9481 accctgtatt cttccaaagt cttatcatat accaatttat ctgcactaaa atccattact
     9541 atttcaccat aagaaggcca ttggataatt gtatcttcac taaaagaaaa ggtttcaacc
     9601 gtttctccat ttgcttcttc tgtaccaata aaaacaggct cttcatcgct tttttcaaca
     9661 accgtcaaag gagctggatt attgtttgct tgtgtctcta aagcaaccgt aacttcctct
     9721 tctttagcag tttcattatt ttctccttca gcttcatctg tccggccggg ggaactttct
     9781 tctgccacag cagcccctga gcctgctata tcctcctccg taatcgtcaa atcattttga
     9841 taggcattaa tcgtacccac atcttccaaa tcaaaagtgc catattcttc atctaaatca
     9901 atatagtttt cttcccgtac tttttctaaa gtatttaaat tattatttgt aaccatcgtt
     9961 gtaactataa ttgttaataa acataataac aacattccat aatagccctt cttattaaaa
    10021 tacgtccaaa attttttact tttcatacta tacacctccg tgtatagtat aaccgaagct
    10081 aaaacaagct attcattctt aaaaagattt ccctctatat cctctaaaga tttaatctca
    10141 accccttgat aataatgttt taatatatct gtataaacct tcccttcctt agccatataa
    10201 tttgccccgt actgactcat gccagcccca tgaccatacc ccttggtaac aaaaataaga
    10261 gtgtctccgt cttccttaat ggtaaagcaa ctggatttta aatttaaaag gtatcttact
    10321 tcttctcctg agaaaatttt gttcccaatt tgaatctttt ttatatagcc cccttcagtt
    10381 ctttcaataa tctgaatttg tcctattaaa tcctgaatca taatatccgg gtatttcctt
    10441 ttcaaacttt ccacaacttc ttttttcaat atcctttttt cttccataaa agctggtgac
    10501 tcttcttctc ctacgctctc tacactttgt aaataaggga catccacttg ccatatatcc
    10561 ctggcacttt gggtaatccc tgcactggtt gaatgaaaaa cagcctcaat aggctcctct
    10621 tcataaagca taatttcccc ttttgtttct tcaacggctt gagaaatttt attataatag
    10681 gcataaaaat tatcccccca tttttccttt aattgttcca atgataaata tgcctgtccc
    10741 ttaaaagaat ctgttgaaat atccgctcct tctttatttt tattttgttt catttttctt
    10801 atagcataag tcctggccgc tacagcctga gccttgagag cctctaattc aaaagaagct
    10861 ggcatttctg cagccactac ccctttaata tattcttcaa aatccatttc cataatttcc
    10921 tgactttctt cattcaacac ctttatcgtt aatatattat tatcctcaat actttctttc
    10981 tcacttgtaa tcgcaccccc ccgttttatt cccttcaaaa aacacagtaa ttaaagcagg
    11041 tagtccaata attgcaaaaa aaatcattaa aaaaagaatg cttaatttct tcaagctatc
    11101 ccccctgcta tgcctatctc ttctaatatg tcttgtacat ttatatgtat cattaataaa
    11161 tatcattctt ataaaattga tgtcgcttcc caaataaaaa agcctccaga aaaactgaag
    11221 gctatttaac cttttccaaa tcagcgccta aagcaattaa ctttttgtcg atttcagaat
    11281 atcctctttc aatatgataa atatcgctga tttctgttgt ttcctcagcg actaaggcag
    11341 ataaaattaa ggaggctcct gctcttaaat ccgttgctct tacctgagtc cctgtcaact
    11401 tctttactcc ttctataatg gcacttctgc tttcaatctt aatctgtgcc cccattcttt
    11461 ttaattcccc aacatgcata aatcgatttt caaaaaccgt ttcaataacc atgctggttc
    11521 cttctgcaat acttaaaagg ctcataaaag gagcctgcat atccgtagga aaaccaggat
    11581 aaggcaaagt tttaatatca accgctttaa taacatcttt tccgcaaacc tttattcctt
    11641 tttcactttc tgtaacctca acattgcatt cttttagctt agcaataaca ggctttaaat
    11701 gatcactgac aacattttca agaaaaattt ctccccttgt aattgcacca gcaaccataa
    11761 aagtccctgc ctctattctg tctggtatta cattatgact ggtaccttta agcttatcta
    11821 caccattaat cttaatagaa tctgtacctg ctcctctgat atctgcaccc attccattta
    11881 aataattggc cagatctaca atttcaggct caactgctgc attttcaata atcgtggtac
    11941 cttcggcata aacggctgcc attataatat tttccgtagc cccaacactg ggaaaatcca
    12001 aatagatctt agcgcctttt aatttatttg cataagcttc cacatatccg ttttgctgct
    12061 taatatctgc ccccaaggca gcaaaacctt taagatgaag gtcgacaggc ctgcttccaa
    12121 tggcacaacc accagggaga ggaactttag cctttcctat tcttgcaaga aggggaccca
    12181 ttactaaaaa agatgccctc atctgactgg ctaaatcata agctgcttcg tatccattaa
    12241 tattatcagc acaaatttca ataaatccat taactttatc ccatttaact tctgctccaa
    12301 aatatttaag caaatcttgc ataatcacta catctttaag aaagggaact tctccgattt
    12361 tactttttcc ttctgccaac aaagaagctg caagaatagg aagaacagaa tttttagacc
    12421 cactgattct aacagtacct cttaaaggcg gacttttacg cactatcagc ctcatataat
    12481 tctctttcct ttctagtatt ctaccgtgat taaaggagtt gccagataaa gataggtttt
    12541 attttcatat tcattatagc ggaatgcaca attgacatta attttctttt gatttgaagt
    12601 aatatactca tcaattaatt ttgtatagcc atagacacta taaatttttt ctgtctcata
    12661 gacttcttca acccttgcat caatattctt cataatactt ttgcatattt tgtctctttc
    12721 tttttcagtt aactttcctt tcatgtgccc cataatcgta atattggtat ttgctttaat
    12781 atcatttgct gcatatattt tatccagctt ttctttaaga taggctaaac ccttatactt
    12841 gtcatatatt ataatatcaa tcactaaata agtgttggcc gtgtccgata attcatcttt
    12901 tatggattgc agctttacag ttgtcttggc atttttagaa gctctgataa cagtaaattc
    12961 tctaagactt tctgattctt tttcctccac atgatcaaaa ttcgaaattt ttaaatcgtc
    13021 cgtaaaatgc ttcgataact cccgcatttc ctcgagagtt gaaaaagaat tattgagtgt
    13081 tccccaagta ttaagattaa tttgaatagg ctgaaactgt gagttgttaa atgtagtaac
    13141 taaagtttct tcttcaaatt tagtatttcc tctttcgaat aaagatacac tataaagaat
    13201 tagtaaaaca gctaaaaaaa tactataatt tctcttcata taaatcctcc aatacatata
    13261 aatccttatt catttaaagt atggacaaat aacattcatt tattcatcat atgaattttt
    13321 ttccaaataa tcagaaaatt ccttatttca acttttcaca aaaaattgtt tacaaagttt
    13381 gtaatttttt ctattatata gctataaagt gagaaaaata atttacttaa tggtgaaatc
    13441 tatatgcaaa atgcattaaa agccttacat caactattaa acatattctt tcaaataacc
    13501 atcataattt gtcttatcaa aatgctcaaa tcattcttta actgaaaaaa gcagggttaa
    13561 gccctgcttt cataaactaa tataaagcca tccagctcat ttattaatac ataccctcta
    13621 aaaaaattca taagaaatcc ctccaaatat agttgacata aatttttcaa ctatgttttt
    13681 ttacccaagt attcatgtaa attcaggttt tctttaattt ttccgtttag ctttccttgt
    13741 ttgagcgaag aaagtttgga aagttatttt cccatgatac tttcgaatct gtcatcccgg
    13801 aggtacgttc tttataaata tggtttgggc gtccaataac aaataaatta aaaaaccata
    13861 attaaaaagg gctatacact aattcattag tgcacagccc aatattttat tcataattca
    13921 aaaaccataa acttttgtta ttataatcat gacaaaattt actcgcgata ctgacacagc
    13981 tctaatctca ctaaagcccg cttcaacgcc gcttcagccc tggcaacatt tatatcctcc
    14041 gtctttttgg tcaacctttc ttcagccctt ctttttgctg cttcagctct ttccttgtca
    14101 atttcctcag gccattctgc cgcatcggca agtatagtta tcctatctgg ttggatttcc
    14161 gcaaaacctc ctattaaagt ggctcttttt tcctcttcac cttgtttaat cctgataatc
    14221 cctatagaaa gaatcgttgt cagaggggca tgcccaggga gtacacccat atccccctga
    14281 atggttctaa aaataaccac atccacctct tcatcaaaaa aattcctgtc tggtgttata
    14341 attttaagca aggttttctt ttccgccata caatcaccat cctagcgctt attgcaggcc
    14401 tttagctttt tcaactgcat cgtctatggt accaaccata tggaaagctg cttcaggaag
    14461 atcatcatgt tttccttcta agatttcttt aaagcctctt atggtttcag aaattggtac
    14521 atactttcca ggcagaccag taaagttttc ggctacaaag aatggctgtg acaagaaacg
    14581 ttgtatcctt cttgcacggg caacaatcag tttatcttct tcagataatt catccatacc
    14641 taacattgca ataatatctt gtagttcctt atatctttga agtacttcct gaacgccccg
    14701 ggctactttg taatgctctt cacccacgat agccggatct aacattcttg atgtagaaac
    14761 aagtggatcc accgcaggat aaattccaag ctcaacaata tcacgggaaa gaactgttgt
    14821 cgcatccaga tgtgcaaaag tggttgccgg tgccgggtct gttaagtcat ccgcaggtac
    14881 gtatatcgcc tgaactgaag taattgaacc tttttttgtt gaagtaatac gctcttgaag
    14941 agcacccact tccgttgcaa gcgtaggttg atatcccaca gcactgggca tacgtcctaa
    15001 aagtgctgat acttcagaac cagcctgaat gaaacggaag atattatcaa taaacaataa
    15061 tacgtcctta cctgttctgt ctctaaaata ttctgccatc gtcaggcccg taagacctgc
    15121 tctcattctt gctcccggtg gctcattcat ctgaccaaat accatggttg ttttgtctaa
    15181 tacccctgaa ttcgccattt cgtaataaag gtcgtttcct tctctggttc tttcgcctac
    15241 acctgtaaaa acagaataac caccatgttc agttgcaata tttctgataa gttcctggat
    15301 caataccgtt ttacctaccc ccgcaccacc aaaaaggcca atttttccac cttttgaata
    15361 aggacataaa aggtctacga ctttaatacc cgtctcaaaa atttctatgt ccgtagattg
    15421 ttccgtaaat tctggtgctg gtctgtgtat aggccatttt tcctcaactt ctggtgctgg
    15481 cttattatca atgggctgac caagaacatt aaaaattctg ccaagtgtct tttcccctac
    15541 aggcacacta atacagtccc ctgtatcttt tgcttccatt cctctcatta aaccatctgt
    15601 agaagacata gcaatgcatc ttacaatatc atctccaaga tgttgtgcaa cttctgctgt
    15661 taatgtttcc ccattttctt tctcaataat gatggcatta ttaagggctg gaaggtgttc
    15721 gggagaaaac tttatatcca atacagggcc gattatctgc acaattttcc ctatatttgc
    15781 atccgccatt attttcactc cttcgctgat taatgactta ttttaaagct tcggctccgc
    15841 ttactacctc agttatctct tgcgtaatgg cagcttgcct tgccctatta aaatgtaaag
    15901 tcaaattatc aatgatttct tcagcgttct tcgttgctga atccatagct gtcattcttg
    15961 ccccctgctc actggcagca gcttcaaaca ttgctccata aataacactg gcaatatatt
    16021 tgggaataat atattctaaa acactttcag gagaaggttc ataaatcatc aaagtacgtc
    16081 tttgttcctg gctttcttcc tgaaaatctg aagtatcaac gggcaataac tttttaacca
    16141 tcggtttttg atctatcgta gacttaaaac ttgtataaac taaatagact tcatcaactt
    16201 ttccttctga atacaattgc ataatattct cttctatttc tttagcattt gaatactttg
    16261 gctcctcaga aatttttata taggccttgt taatcttata gcctcttctt tcaaaaaaat
    16321 cttttgcttt cctgcctaca gtaattagtt ctatatcagc cttatttctt tcttccatat
    16381 atttaagagc taatttacag acattattgt tatatcctcc tgctaagccc ctgtcagaag
    16441 ccataacaat atacaaggtc tttttgactt ctctttgctc cagatatggg tgacttatcc
    16501 ccttactgct gttcacaata gaagagattg tcttttgtac aatgttgaaa aagggtctcg
    16561 tgctttctaa atcttttctg gctttttgca tctttaccgt tgatactagt ttcatagctt
    16621 ttgtaatttg acctgtactg tttacacttc ttatacgtct tttaatttca cgcatggagg
    16681 ccattataat acccccttac atttcatttt taaatatttt cttaaattct tttatagcgc
    16741 catcaatttt attttctaaa tcactgtcta tttttttctc agttcttatg gtatccacga
    16801 tatccaaata ctttgtatcc ataaattcga ggaactcttc ttcaaacctt ctgatgtctg
    16861 ccacatcgat atccattaaa tatttccttg tcactgcaaa taaaataagt atttgatgtt
    16921 caacagacat aggcttgtat tggggttgtt ttaaaacttc tatgatacgt tccccttgag
    16981 ccagggcatc tcttgtcgct ttgtccagtt ctgaaccgaa ctgagcaaaa gattctaaat
    17041 ccctgtattg ggctaattcc acacgaatag gccctgcagt ttttttcatg gctgctattt
    17101 gcgcagcgcc tcctaccctc gatacagaaa gaccagggtt aatagcagga cgaatacctg
    17161 cattaaataa ttctgtttca agataaattt gtccatctgt aatagaaata acgttcgtcg
    17221 gaatataggc agatacgtct ccgtcctggg tttcaataat aggaagagcc gtcagtgaac
    17281 cgccacctct ttcatcggaa agctttgcag cgcgctccaa aagtcttgaa tgcaaataga
    17341 aaacatcacc tggataggct tctcttcctg gtggtcttct aagtagtaag gacatagcac
    17401 gataagctac cgcatgcttt gaaaggtcat catatataat taatacatcc ttaccttctt
    17461 ccatccattc ttctcccata gcacatccag cataaggtgc tatgtactga agaggagcca
    17521 attcactggc tgtagcagac acaactgttg tataatccat tgccccatgg tcttcaagaa
    17581 cctttaccag ctgtgctact gttgaggctt tttgtcctat agcaacataa atacacaaaa
    17641 catcttgtcc tttttgatta ataatcgtgt ctattgcaat agctgtttta cctgtttgac
    17701 ggtctccgat aattaattct ctttgacctc ttccaatcgg aatcatagaa tcaatagctt
    17761 ttattcctgt ctgtaaaggc gtatctacag actttctttc aataactccc ggagctattc
    17821 tttctacagg tctgtatttg gtcgtatgaa taggaccttt tccatcaata ggctgtccca
    17881 aagcattaac aacccgtcca ataagggcat ctcctacagg tacttcaaca actctccctg
    17941 tagattttac aatatcgcct tcttttatcc ctttatctga acctaaaaga accgctccaa
    18001 tattatcttc ttctaagttc agggccatac catatacacc cccgggaaat tcaagcagtt
    18061 cccctgctat ggccttttct aaaccatgaa ttcttacaat tccgtcacct acttgtaaaa
    18121 ccgttccaac gttagaaact tctaatttgg tctgaaaatt ttgtatctgc ttcttaatta
    18181 ctgaactgat ttcttctggt cttagattca tttttcttta caccccttta caccagttgc
    18241 gaatcataca ggcttgcttt taaagcttga agcctgcctc caatacttgc atctaaaata
    18301 ctgtctccaa ctctgataat aagtccccca atgatagaat gatctacctg agtttcaatt
    18361 tgaatttgtt tctttaatcg ttttgtcagc agcattttaa tctgactgat ttgttcattg
    18421 tctaaggcaa ctgcagatat caccgttgct gttacaatac ccttatattc ttttacctta
    18481 tctaaaaaag catttaaaac atctaacaga tattcttctc tgtttttacg gataattaac
    18541 actaaaagcc ccataatttc actggatact ttggcttcaa atactttttg caaaagttca
    18601 atcttttctt ctgcaataat ttgaggatgc tgcaaaagct ctgtgaaatc tttttcagtt
    18661 tttaatattg tataaagact gcctacctga ctttcaaatt catcgatttt attgctttca
    18721 acagccaaat caaacaaggc ttttgcataa cggtcagaaa ttagctgtcc catgatacat
    18781 cccccatttt agaaatggct tcatcaatta atgctttctg tttttcctcg tctatggacg
    18841 cagcaataaa cttctcagca ataagagaag cgatttctat gatttctttt ttcatttcgt
    18901 cctgtgcttt tgctttctcc ctttcaattt ccgctaatgc acgttttctt atcatttctg
    18961 actcttttct ggcttcttct ataatgctgt tttccttctt taatgctttt tctcttgctt
    19021 cttttataat cgcatctgct tcttcctgta tcttttgtaa ttttgcttca tattcttctt
    19081 ttaactttaa cgctttttct tcttctgccc tggcaaaatc aatttgtccc tttatttttt
    19141 cggaacgctt gtccataaat tcggttaaag gtttaaaaag aagcttacca aggaaaaaaa
    19201 agagaatcag tgtgttgata agttgcactg ctaaactaaa taagaactgc ttatcaaaac
    19261 tgagaagact tccatccaag agaaaaatcc tccttcctct gtctaaccat ctgcaatagg
    19321 atttattatt taatatgaaa tgaaggggtt tgaccccttc ataattattg gcctaataaa
    19381 gaaaggtatt tgtttattaa tggatttgca aacactaaaa taatagcaat gaataatccg
    19441 taaatacctg ttgtttctgc aactgcgtct cctaataaaa gtgttcttat aatttcattt
    19501 tgtgcttcag gttgacgtgc aactgcttca gctgctttac ctgctgcata cccctgacct
    19561 attcctggtc caatccctgc aattaatgca aggcccgccc ctattgctga acatgctaat
    19621 attaaagctc ttccgtcaat tccttccatt ataaatacct ccttatttta tattattgtt
    19681 ttatttttta ttgaaactac tttcttttca gaaaatagct tgcagcttaa tgatacttat
    19741 tcttgaatag cattagaaac aaaaatcata cttaacatta caaaaataaa tgcctgaagt
    19801 gcacctgaaa acacatcaaa atatacatgc aacactgctg gaacgcctat tttagtaaga
    19861 cctggcaaag ctgcataaat aagccccata ataatcacgc cggctacaat attaccaaaa
    19921 agacgaaagc taagtgatac aggtgttgcc aattctccaa tgacgtttaa aggaagaaga
    19981 aatggtaaag gttcaaaaaa acctttgatg taatttttaa taccgccagt tttgatgcca
    20041 taatactgaa ccataacaaa agtcattaat gccaatccaa aagttgtcgc caaatcagat
    20101 gttggcggtc tcatgccaag aagtccccac aagttggaga aaagaagaaa taaaaacaca
    20161 cttccataaa atgcagcaaa tcttttattc ctttctccca tagtgcttat tgtaaaagta
    20221 ttaacaactt ctccaataat ctctactaca ttttgaaaac cttttggtac ttgtttaaaa
    20281 cgggaaatag aaaacctgac aaccaaagca aaaataataa gagcagccat tactatccat
    20341 gtacttataa tactttgagt aatgtaaaat tcttctcccc caatatgaac tggaaaaaca
    20401 tacttattag aaaaatccaa accctccatt attaacactc cttcccaata ttaacttaat
    20461 gaaaagcata ttttattaaa aattaatgtt ttgaattgtt ggaacccttt ttatctgtaa
    20521 tcaatgttaa ataaacagaa aacttaattg taaataagcc aataatagta gctattaaat
    20581 taatacttgg ctcaagagct gctacaataa gcacaatggc cgtcagaaaa tatctgataa
    20641 aatattgaga tgacgcataa cggcttgctt ttgcggggga catttttatt gcctttttta
    20701 tcgttgcttc tattaaaaac aaccttagaa tcgatataat cgttccaaaa aaaagcccct
    20761 ttatccaggc aaacggttca ttcactaaaa aaagacctga aatcccaata aaaaaactta
    20821 tcgctattat ttgacgaatg agccgtttca tcatttttga tcctttcttt tatccccatt
    20881 ctttaatgct tttgtggtta aaatatataa attccgaaat gccgctccta ttcctaataa
    20941 agaaaatatc aaaagaaaga caatccctgt gtttaattta ttatctatat agtttccgaa
    21001 aatcacacca aatataatag gtactgccat cataaaacca atttgagaaa tgagagagag
    21061 agccgttaga aaatcaaact ttttcttcat taaaattcac ctgggaaatt aaattaagaa
    21121 caattaaaaa agtgtagata ttataaatta tacacgttta ttctttgagt gtccagtaat
    21181 acaattttca aaaaatgata aaatccttcg gtctttcgac aatcttacca aagctgtata
    21241 agattgcatc gacaattctt cttgaagcat ttccgtctcc aaaaggattt ttagcctttg
    21301 tcattttttc gtattcctct ttattttcca aaagttcttt tgccatatta aatactctgt
    21361 cttcatccgt tcctgctaat ttaagggttc ctgcttttat tccttctggt ctttctgtta
    21421 catttctaag aactaataca ggttttttca tagaaggcac ttcttcttgt aagcctccgg
    21481 aatcggtcag gacaagataa gataaattca ttaaattatg catatctttt aaatctaaag
    21541 gatctatcaa atgaattctt gaatgattgc ccaagatcgg ataaactatt tctcttacag
    21601 cagggttttt atgcaccgca taaaccactt ctacatcatc aaaagcttct actaatctgt
    21661 atacgccctt gcaaatattt tccaggggtt tgcccaggtt ttcccgcctg tgggctgtca
    21721 tggtaatcac ctttttattt ttataatcaa tttcatttaa caagggcaga ctaaaaacat
    21781 aattatcttc aatcgttgtc tttaaggcat caattaccgt attacctgtt ataaaaattt
    21841 gttcttcaga aatagcttct tttaataaat gctcttttgc taaaggagtt ggtgcaaaat
    21901 gaaggtctgt cagagaacct gttaattttc tgttcatttc ttcagggaaa ggctgatatt
    21961 tatcataagt tctaagacca gcttctacgt gacctacttt aatttgatta taaaatgctg
    22021 ccaatgcccc tacaaatgtt gtactggtat ctccatgaac caatacaatg tcaggaacaa
    22081 cttcctttat aaccttatct agaccttcta aagcatgaat cgtaatatcc ttaaggcttt
    22141 gcctttcttt cataatatct agatcataat cgggtacaat attaaacact tctaaaacct
    22201 ggtctaacat ttccctatgc tgtgcagtaa cacaaacaat actatctatt ccaggagtat
    22261 tttctaattc ttttacaact ggtcccattt tgatggcttc aggtcttgtc ccaaaaatac
    22321 tcatgacttt aattgacatg gatatttcca acctttctgt ctttaataat atttaatcat
    22381 atctctttta tttctgcaat aaatataggg attattgact atcttaccaa tggctatttt
    22441 cttaaatgca aattaagttt ttataattcc acttgtaata ataatctaaa aattataaaa
    22501 gacatactta agagtatgcc ttttatatgg ctatttattc cgtgttatca tgtaaagggc
    22561 aattcccatg gctataaaca cccctaaggc catatgtgta tctttaagtg ccagtaatat
    22621 accaatgatt ccaaaacctc cacttattcc gtaaagggta attacagcct gcttttgact
    22681 ataacccttg tccaccagac ggtggtgaat atgtcctctg tctgcttcca taataggtct
    22741 tctgctaata attcttctaa gaattgcaaa ggtcgtatca aagattggca gtgctaaaac
    22801 caatactgca ataagaatgg taactgctgt atatcccttg ataagaccct gaatggaact
    22861 aacagccaaa acaaaaccta aaaatgtcga gcctgtatca cccataaaaa tctttgcagg
    22921 attaaaatta taaggtaaaa atccaagaca agagcctgct aaagctgcca ttaagattgc
    22981 tgcaaccgga ccaataggat ttggagaaaa catagataaa atcataagac atattgcagc
    23041 aatagaagat acacctgcag ctaatccgtc taaaccatca attaaattaa cagcattggt
    23101 aacgccgaca atccagaata atgtaatagg tatacttatt ttttccaata tcaaaatatt
    23161 accttctgca aaaggccagg taacaaactc aattctaata tctgaatata taacaactaa
    23221 agctgcaata acttgtatta aaaattttaa ttttgcattc agattataaa tatcatcaaa
    23281 aaagcctaat aaaaatataa tcattcctcc aacaagcaat cctgaaatct gcttccattc
    23341 aaaagcttct acagatggag taacaacaag aacagtaaat ataaatccta aaaatattgc
    23401 tattccaccc attcttggca ttggcttatc atgcattcct ctggctttag gctgatcaac
    23461 cgctccaacc ttaaaagcaa tcattttact taaaggagta gtcaataaac taattaaaaa
    23521 tgccatgcca aaagcaataa aatatattaa aaatttatct tgcatgataa ttccctgaaa
    23581 taatttcatc ggacatcgtc tccttaaatt tcacctatat atattttccc ctattcttaa
    23641 tataataaag catacagatt gttacatatt aaaagccgta ttaataaggg agaaatatac
    23701 atatattaaa ccacaattat atagtttata aaactattaa tatacatttt tgttaaaata
    23761 taataattta ttttactaaa aaattcaaac acattatgta ttctattaaa taatgagcca
    23821 tgtatattct aagattttat gtaatatttg tcaaattaaa tttttgttac attttctttt
    23881 ataacttttt taaaaaaccg atactaaata atagtatcgg attttatatg cctgttattt
    23941 ttatccttta aaattcacga cttctatacc tgcttcttct aaaagttctc tggaaagctc
    24001 gtcaggatac tctcccttat acacgattct ttttatacca gcattaattg ccatttttgc
    24061 gcacatcaca caaggctgat gggtaacgta taaagttcct ccctgtacag atataccata
    24121 caaggcagct tgcacaattg cattttgttc cgcatgaagt gccctacaaa gctcatgtct
    24181 ttgccctgaa ggaatttgta atttttctct taaacagccc acttcttcac aatgactgca
    24241 acccgtagga gcaccattat aaccggttgc taatattctt ttatcattta caataagagc
    24301 gccaacttgt cttctaagac aagttgacct ctcttttatg agttcaacaa tttccatgaa
    24361 atattcatcc cagcttggtc tcacaaaatc cccccaacta ctttgttcca aacaatctgt
    24421 ctcctgcatc ccctaatcct ggaacaatgt atccatgatc atttaatctt tcatccaata
    24481 cagcaacata aatatctaca tccgggtgat ctttcattaa cctttctacc ccttcaggag
    24541 cagcaataag gcataaaaat cttattcttt gcacaccctt gtccttaata aactgaatcg
    24601 cagcagatgc tgagccacct gttgccagca tcggatctaa aacaaatata tctcttgttg
    24661 gggaatcata gggcaattta caatagtact caactggttg taaagtttct ggatccctat
    24721 ataagcctat atgccccact tttgctgcag gaatcaagtt aagcataccg tcaaccatac
    24781 ctaaacctgc ccttaaaata ggaacaattc ctaacttttt accagacagt actttggctt
    24841 ttgcctttgc taccggcgtt tccacttcca cttcttctaa aggcaaatct cttgttgcct
    24901 cataacacat aagcattgat atttcttcta ccaattctct aaattccttt gaacctgtat
    24961 tcttatccct taacatagaa attttatgtt gaataagggg atgttccatt acatgtaatt
    25021 gccccatgtt attcctcctt acataatttc aattattatt cttcaatttg tgaaatacgt
    25081 cttacatgtc tttcatc
//
LOCUS       DAOSUD010000027        23755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1394_length_23755_cov_24.502743, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000027 DAOSUD010000000
VERSION     DAOSUD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 23755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23755
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_23755_cov_24.502743"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..455
                     /locus_tag="PLG49_05140"
     CDS             222..455
                     /locus_tag="PLG49_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOQ16741.1"
                     /translation="MDNIIVYSTPTCPFCYMVKDYLKEHNFQFKDINVAEDPVAAQDM
                     VRKSGQMGVPVIDINGNIVVGFDKNKIDSLLGL"
     gene            708..4208
                     /locus_tag="PLG49_05145"
     CDS             708..4208
                     /locus_tag="PLG49_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOQ16742.1"
                     /translation="MKKSLSFLLSFLILVSTFLFNTQKTEALEMNEEARERKSYERSL
                     SVFEYVYEKNPFFEEEFFDFVMVQEAVYEKPNLVITDLTTVHEDIQDYKLVPFTVYIE
                     NTGGMTREDIYLTWKIDGKTVGTTVLERGMFPYERRSSTFEWRAVPGNHILTVVVDER
                     DYIEEEDEGDNIFEKELYVRDALKLNLNRYSVDRPFVQTLDTGSGFSVAIRKDGKIAF
                     TGNGREGRSNVSLWEDIVQLSAGAAHIVGLKADGTAVVAGYNADNLYNVGGWEDIVQV
                     SAGYDHTVGLKRDGTVIAVGLNNYNQCSLEDWEDIVQISAGESFTVGLKADGSVVAKG
                     INNQGQCNVGDWEDIVQVSAGMNHTVGLKSDGTAIAVGNNQYGQCDVERWSELVHVWA
                     GDKVTIGVRKDGSVLACGNNDYGQTNVSHWTDIVQAFAGNNFTLALRQDGKVLATGDN
                     RKGQCNFAGVEWPDAEDIIQVSAGTNHTVLLRSNGKVIAIGANNYGQCDVSEWEDIIQ
                     VAAGEDHTVGLRRDGKVVAVGRNLKGECDVEDLEDIVQISASSSSTAALRSDGKVFVL
                     GYGASNRINNWEDIIQVSSGYLRTVGLQRNGRPVAEGYNYYGQNNIENWEDIIQVSAG
                     ENHTLGLRQDGKVLAAGRNYNGECRVSDWEDIIQVAAGENYSLGLRADGRVEQAGSNF
                     DLSEWENIVQISAGDYHAIALKRDGTVIGAGDNTYGQSKEIAAEILSVPELFREEAFS
                     LEVTELSYSFDESYNEEDYCDGMNMTIHAEIMNKSSQLIDSTAVLFKLDDHVVGSKTI
                     NQFQPGEKRTVSLSCLFDAGAKNLIVIPDSRFPDNAVTTLSLPDVEKAELSLSVRIEN
                     YNNRFIVYASVSNEGNTTIRNIDIDYVLDGEKIGSSTIRNGIKKGETRISSLQFYTTA
                     GTHQLKVLVDPQNIVPEENKENNDYTEEITAVIIPGVEFLNLERGQKITSPYQIEWQA
                     ASQHYNPDELKISLSLINDKGEFLIAKDIENSGFYNWSFPDIPEGEYNLRIIATDPKG
                     NASEASRLIRIINPARIEITHNSYKMIILEGDEAKLDLLVKNLQPQEDRIILSIEKDK
                     SLVMDDDIILTMEPWEERWIQLDISYPSCGLYQINIVAESENIPSIRCEETVELAVLP
                     PVLQSFFEHLRRFFAEMNLL"
     gene            4406..5071
                     /locus_tag="PLG49_05150"
     CDS             4406..5071
                     /locus_tag="PLG49_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ16743.1"
                     /translation="MEKITYTDIKNNPEINTYIKKGDELLEIMGYTDHSEIHAAKVAE
                     TAAEILRKLGYSERECELAKIAGYLHDIGNAVNRVDHAQTGAVMAFNILSKLNMDPEE
                     IATIVAAIGNHDESTGTAVSPVSAALILADKTDVRRSRVRNKELVTFDIHDRVNYAVE
                     KSKVIIDEEKRKLILELTIDMRISSLIEYFEIFLTRMLMCRRAADFLNINFELVANEK
                     KMV"
     gene            complement(5072..5377)
                     /locus_tag="PLG49_05155"
     CDS             complement(5072..5377)
                     /locus_tag="PLG49_05155"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOQ16744.1"
                     /translation="MKSYFFIILGMAVVTFIPRLLPFIFISKKEIHPTLKRFLNYIPY
                     ATLGALILPGVFNAIPNQPKAVWAGIVTAVFLSWWKGNIILSVLASVGISFLMIQYF"
     gene            complement(5374..6081)
                     /locus_tag="PLG49_05160"
     CDS             complement(5374..6081)
                     /locus_tag="PLG49_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOQ16745.1"
                     /translation="MNKYYNGEVLSGFKDALPIAIGYIPIALAFGIISKANQISLWDC
                     FLFSLFVFAGASQFMAVNLIAATVPAFQIILTTAVMNLRHFLMSASLADKIKSPRKKL
                     IPLVAFGITDETFSVLATKNKELSFFYTLTVNTTAYFSWVLGSVAGYLLGSVLPEAIQ
                     SSMQIALYAMFIAILVPSMKKSKEVIFLALLSGGIHAFLNISHLIPEDWNLILSITLA
                     ALIGLMILPKEEGGVNP"
     gene            6233..7063
                     /gene="thyA"
                     /locus_tag="PLG49_05165"
     CDS             6233..7063
                     /gene="thyA"
                     /locus_tag="PLG49_05165"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOQ16746.1"
                     /translation="MSQADKIFIEMSESILNDGFSSEGQDVRPRWEDGTPAHTIKKFG
                     VVNRYNLAEEFPILTLRKVPFKRAIDEILWIWQKKSNNIKDLNSHIWDAWADENGSIG
                     KAYGYQLGIKHKYKEGEFDQVDRVLYDLKHNPYSRRIITNLYNHQDLHEMNLYPCAYS
                     VTFNVTGNKLNAILNQRSQDVLVANAWNVCQYAVLIHMFAQVSNLEVGELVHVIADAH
                     IYDRHVPIVKELIKRESYPAPKFIINKEIKNFYDFTVDDFELEGYQHGEPIKNIPVAV
                     "
     gene            7073..7570
                     /locus_tag="PLG49_05170"
     CDS             7073..7570
                     /locus_tag="PLG49_05170"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOQ16747.1"
                     /translation="MNIIVSVDQNWAIGYKGDLLQKVPEDMKQFKEKTWGKVVVMGRL
                     TFESLPNQKPLPNRTNIILTRNENFRVEDAIVCHSVDEILKTTKYCNPEDIFIIGGEH
                     IYKTFLPYCSKAYITKFHKVYPADTFFPNLDEDENWRLIEKSGPRQYQDLTFEYITYE
                     NINKI"
     gene            7701..8270
                     /locus_tag="PLG49_05175"
     CDS             7701..8270
                     /locus_tag="PLG49_05175"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ16748.1"
                     /translation="MLKKFQYNAPVILTFSLISFLVLLLSYFTGGLTNQLLFCVYRSS
                     LKDPLFYLRLFTHVLGHVNLQHFVNNFILILLIGPMMEEKYGSKKMLFMIIFTAFVTG
                     IIHILFYNSALLGASGIVFMLILLSSFANIQKGRIPLTFVLVVIIFIGQELVNAIMLH
                     DQISQLTHIIGGICGGFFGYKINNSKKES"
     gene            8389..9561
                     /gene="rpsA"
                     /locus_tag="PLG49_05180"
     CDS             8389..9561
                     /gene="rpsA"
                     /locus_tag="PLG49_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOQ16749.1"
                     /translation="MENNKEMTMAEMIEDIDKSMRKLNKGDIIKGKVIKVNENDVLVN
                     IGYMLDGIIPKEELSDDANINPRDIVSVDDEIDVYVLEVDDDEGNVRLSKKLADQIVV
                     WDELEEYFKSGSPLEVTVKEVVKGGVVANIKGVRAFIPASQLSVHYVEDLNRYLGKAL
                     KVKLIDFDRKSRRVVLSAKEIEQAEREIRKKEIWKSLKRGEKRKGVVTRLVKFGAFVD
                     IGGVEGLIHLSDLSWKRVMDPSEVVSVGDEVEVYVLNFDEKKERISLSLKDKAEDPWN
                     NIMDNFKVNDLVEGKVVRLAPFGAFVEIAPGIEGLLHISEISDKRISKVSEVLSEGDK
                     VKVRIIELKPDNKKLSLSMKEAHEDYSEEIKKYNFTQDEDVTIGDLLKDQLKGLKF"
     gene            complement(9609..10046)
                     /locus_tag="PLG49_05185"
     CDS             complement(9609..10046)
                     /locus_tag="PLG49_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16750.1"
                     /translation="MNENNGNRFIWLFVGLFIILFAIAGINHIRGTDDKITASMDEMV
                     KAIVESDLVLSDIPVDIPDESQIPLEYLHLYRLDKETILSQKLVNNYYFIYNPNTKIA
                     SIFYLRDDGTLLLYTSKEGRQFSEKSFAPFKKEIEQIYLQTLQ"
     gene            10216..12192
                     /locus_tag="PLG49_05190"
     CDS             10216..12192
                     /locus_tag="PLG49_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOQ16751.1"
                     /translation="MKVLKVFFKLLLVILISFFISMHIWAQEPGQEDNKGQSAISLEE
                     CIKEMMNIKNEKLDNNQKSYLEAGEERGYLKGIINRNKDEAVTRAMLSRIIVNFLDIK
                     PIYSKDDLFEDLKAFPDAEYIKGVYAEYLIDGIDEKHFYPNQPVSKNEFKTILERIKK
                     YYQNPNNFKAEMKSQSSPKKEEKIKKLLSMTSYKPPKEKVAPGTSLGVLEKGIAQAFI
                     NDYYIPSFSYDNASMIILSDLNYYGFDIIWDETNKKISIIENKNKARSALSEEEIKKR
                     EKNYIGKPLLYTNIAVYIGNRKIPCFLADNSTIIYINDLSIFGNLNWDGQNKIITLQT
                     RDTDINDDLGVELVNQKVINYNEETIDLRMTHLWYDEKVKKVHTVEDSVYGIPSGDAI
                     EFSLEKYDLYEKSYYLGTALKSTNKKDNPFYSREVNYLQSQKMIDYTSKLYKDPEEEL
                     LSLVKASIIIGTMKKNAGPFAKDEKVEILYAEAGSWYQCRSISTKKEGRVPWGSVSIP
                     PDPPTNTKRLTKEQLEAYVKLKGFTSNTNYFVWTDLDRQMTYVFKKENNEWKLIRSML
                     CSTGKNITPTPRGFFTIKERGAYFGKGYMCKNWVQIWGDYLYHSVIMDPTGSYVLEGN
                     VLGKRASQGCIRFSLEDSKWFYDTIPRNTTVWIN"
     gene            complement(12189..12980)
                     /locus_tag="PLG49_05195"
     CDS             complement(12189..12980)
                     /locus_tag="PLG49_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16752.1"
                     /translation="MKGVFKAYKKNGEAYFRSSITYKNKHISLGSFDNSRLAHKAYLE
                     ACEILFKKRYKLEDYNKSKILSFEKWVILCNYRDNGYYFGNPIYLHKYYFSYFLSPDI
                     ELKFDTDDLFYYASHKIHQKGGYFYVNDFGIQQNILTRYGIRNFAVKGIDYRFKNGDE
                     YDFRHYNIEVINPYYGVRREYRRGRMVFVARINIYGTWTIGCYSSPINAAVAYNKAVD
                     FVVSHGISSKSYIKNYIDELNAHDYKEIYEKIKISKRLLKLAKKD"
     gene            13161..15134
                     /locus_tag="PLG49_05200"
     CDS             13161..15134
                     /locus_tag="PLG49_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOQ16753.1"
                     /translation="MGKKKDTYGNDSISLLKGADRVRLRPAVIFGSDGLKGCQHAFIE
                     ILANAIDEAREGFGKKIEVTVFKDYSIMVKDYGRGIPLDYNEKEGRYNWELVFCELYA
                     GGKYENNTGSNYEYSLGLNGLGCCATQYSSEYMDVTVYRDGYKYELHFEKGNNVGGMI
                     KEKFNYESTGTIIKWRPDLEVFTDIKIPLEFFKETLKKQAVVNAGVLFVLNDEASGES
                     FEYCYQNGIIDYISEINSDKGFTSIQFYEHETSGRDREDKPEYKVKFQMAFVFNNEIN
                     LLEYYHNSSFLEYGGSPDKAVRSAFVYAIDQYLKAEKKYNKNEKKITFADVEDSLILV
                     SNSFSTITSYENQTKKAITNIFIQEAMTAFLKKQLEIYFIENKEEAEKIAKQVLINKR
                     SREKAEKTRIDIKKQLGGKVDVVNRVKKFVDCRTKDVSKRELYIVEGDSALGSCKLGR
                     NAEFQAIMPVRGKILNCLKADDHKIFQSEVITDLLKVLGCGVEFSNKYNRDLSNFDLN
                     KLNWDKIIICTDADVDGYQIRTLILTMLYRLVPTLIREGKVYIAETPLYEIRTNKKTY
                     FAYNEEEKKAILSRIKGHYTIMRSKGLGENDPDMMWETTMNPETRRLIKIVPDDIEKT
                     KEIFEILLGDNLEGRKEYISEYGHLYLDPTDLA"
     gene            15158..17329
                     /locus_tag="PLG49_05205"
     CDS             15158..17329
                     /locus_tag="PLG49_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOQ16754.1"
                     /translation="MGEKTTTIAEKITDTLKKNYMPYAMSVIVSRAIPEIDGFKPAHR
                     KLLYTMYKMGLLKGGRTKSANIVGQTMRLNPHGDQAIYETMVRLTTGCESLLVPFIDS
                     KGNFGKQYSRDMAYAAPRYTEAKLEEICEELFKDIDKDTVDFVDNYDGTMKEPLLLPA
                     TFPNIIVNPNQGIAVGMANNICSFNLKEVCQATIAYLEDEKVDISKYIKAPDFPTGGN
                     IIYNAEEMKEIFETGRGSFRIRAVYNYDKKSNAIEITEIPYTTTVEAIIEKLVSLIKA
                     GKIREINDIRDETDLGGLKITLDLKRSTDPDELMRKLYSLTPLEDSFNCNFNILVDGH
                     PRVMGIKEILYEWTKFRINCIRRRTQYDINKKSEKRHLLLGLEKILLDIDKAIKIIRE
                     TEKEKEVIANLMKGFNIDEVQAEYVAEIKLRNLNKEYILNRTKEIESIDKELKALNVL
                     LKDEKKIKKVIAQELEMVAKKYGRPRKSKIISEEKIETLSKEDFIDDYNVKIFLTRQQ
                     YIKKISLVSLRAYSTQELKEGDEIIQEIEATNKSELLLFSDKQNVYKIKAYELEDTKA
                     SSLGDFIPNIVELEEGENIIFVAAASDKYEGDLLFAFENGKLVKIPFRLYETKQNRRK
                     LINCFYGRSKCVAILHITEDIDILLKRSDNKVALINTSLVVKGNNKKAAGNQAVKVTK
                     KSRLEAVLTVEDFSGNDVEQYRATSIPAKVQSIYTQIELNI"
     gene            17346..17885
                     /gene="hpt"
                     /locus_tag="PLG49_05210"
     CDS             17346..17885
                     /gene="hpt"
                     /locus_tag="PLG49_05210"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOQ16755.1"
                     /translation="MKNDIKEILFSEEEIAKKVKELAQKITEDYRGKDLTVIGILKGS
                     NIFMGDLIRKIDIPLQIDFMVVSSYGQSTESSGIVKVLKDLDYSIEGKNILIVEDIID
                     TGLTLHYLKGILQKRNPNSIKICTLLDKPARRKVDLEVDYVGFQIPDEFIVGYGIDYS
                     EKYRNLPFVAVLKDEVYKK"
     gene            18247..19260
                     /locus_tag="PLG49_05215"
     CDS             18247..19260
                     /locus_tag="PLG49_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ16756.1"
                     /translation="MEEKILKAKAGMPCLILFIFLYLAAIASIIFGGNLLSKQNNLGA
                     VFLTIGIIWVSIGFVPFLGLKIIKPQEALVLTLFGNYIGTLKEAGFFFVNPFAVAVNP
                     AAKTRLGQSDDVSHIPSIKLEAGINLQNNYVSKKISLKVMTLNNKKQKINDALGNPIE
                     IGVAVIWRVVDTAKAVFNVDNYKEYLSLQCDGALREAVRMYPYDIGLVEDNGANGEAD
                     EFSLRGSSKEVAQRIRETIQAKVENAGIEILEARITHLAYAPEIAAAMLQRQQARAII
                     DARKMIVDGAVGMVEMALEMLNEKQVIQLDEERKAAMVSNLLVVLCGNRDAQPVINSG
                     SLY"
     gene            19262..19447
                     /locus_tag="PLG49_05220"
     CDS             19262..19447
                     /locus_tag="PLG49_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOQ16757.1"
                     /translation="MDNKKQIPLRISSKLYEEIAAWAEEEFRSVNGQIEYLLTECVKQ
                     RKKNGRPVPKDLGKKEE"
     gene            19703..20893
                     /locus_tag="PLG49_05225"
     CDS             19703..20893
                     /locus_tag="PLG49_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16758.1"
                     /translation="MLRQFICYASIGSLVFFTGWQPCYAEDSSYLENRVIEISAGAYH
                     TVALKEDGTVWTWGNQNFESTDDYDEEQSCNSKAEQVESLEDIKAIAAGYHHVVALKE
                     DGTVWSWGNNVFGQLGHTDFVVRNNQPMMVVDLKDVKAIAAGEWHTVALKEDGSIWAW
                     GDNEDGQLGDGRVIATNKPVQAIDIEEVVAIASGNCFTVALKQDGTVWTWGLYFYYDP
                     IENVDIAMTSTQPVCKEGIEDVKTIAAGSDHTIAIKEDGTVWGWGSNFAGQLGAIVIA
                     DSDTNNEFKYERFMQVSGMEKVKAIAAGYNYTIALKKDGSLWVWGRNNLGNLGDDSIE
                     TISIEPVPVKDLEDVIAVSAADDHIVALKKDGTVWSWGLNLYGQLGDGTVMYTSKPVQ
                     TKIK"
     gene            21068..21622
                     /gene="thpR"
                     /locus_tag="PLG49_05230"
     CDS             21068..21622
                     /gene="thpR"
                     /locus_tag="PLG49_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOQ16759.1"
                     /translation="MRVFVAIDFDNKLKAYLKEKQNELKTYCTKGNFTHEENFHLTIV
                     FIGEVKPEQIPEIEKVMDNAAGQSEGFTLKLSHFDYFKRQDEYLPWIGLDGDLTSLND
                     LYRKLHSKLKVLGFSLEERDLKAHITLGRRVKFNEPVQIIKKNFIIDPITVPVTSIVL
                     MESSRVNNKLTYIPLYEKNLNIRS"
     gene            21726..22247
                     /locus_tag="PLG49_05235"
     CDS             21726..22247
                     /locus_tag="PLG49_05235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ16760.1"
                     /translation="MDKNPVVMICMEDGSQMKVELYPDIAPNTVNNFISLVKKGFYDG
                     LIFHRVIKGFMIQGGDPQGTGAGGPGYSIRGEFIINGFDNNLKHTRGVISMARAQNPD
                     SAGSQFFIMHEDAPHLDGHYAAFGKVIEGITVVDKIASVETDYFDRPLEEQRIKFMTV
                     DTFGKEYPEPEKL"
     gene            22375..22755
                     /locus_tag="PLG49_05240"
     CDS             22375..22755
                     /locus_tag="PLG49_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ16761.1"
                     /translation="MSSYDNAKAMRELRKRRKEKKLCTRCGKPVFGVHVQCDACREYS
                     RIYALLHPKEKVIIRHLKSWEIKNKKLYDILIKKRITIPQLAKMIGVSSRSVDRWVFE
                     GCIPKLENREKVNAYLNMEVFELE"
     gene            22845..23561
                     /locus_tag="PLG49_05245"
     CDS             22845..23561
                     /locus_tag="PLG49_05245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ16762.1"
                     /translation="MCLNTILFDLDGTLLPLDMDTFMNVYFEEVGKLFRDIVNPKELI
                     TNIWKATEEMMRNTEYKTNEEVFTEAFEKYMGRDIRIFINRLNDFYDKEFMRVRKATG
                     DSPFIRKAVYLLKDKGYDIVVATNPLFPKKAIVHRIRWAGFLPEDFRYITCYEESHYC
                     KPNIKFYEEVLEKINKSPKECMMVGNDVKEDLVAGKLGIKTFLIEDYIVDKKECDFLP
                     TYRGRYEDFYKFVYELPEIS"
BASE COUNT         8618 a         3231 c         4805 g         7101 t
ORIGIN      
        1 tttaagtatt tgtataggta ttttatttcc tttgggttag tagtcttggt aagtatgatt
       61 taaaataagt aaaaagataa gaaaaataga gctaattaaa gatattagaa gttatcctta
      121 gtttattatg agaaattagc taaaatgaaa tattgatata tcataataat gtgatataat
      181 aatattaaga tttaaaaaag tgattgtaaa ggaggtatcc tatggataac attatcgtat
      241 attcaacacc gacctgccct ttttgttata tggtaaaaga ttatttaaaa gagcataatt
      301 tccaatttaa agacattaat gtagcagaag atccggttgc tgcccaagat atggttagaa
      361 aatcaggaca aatgggtgta cctgttattg atattaatgg aaatattgtc gttggttttg
      421 ataaaaataa aatagatagt cttttaggcc tataacgctt tataataagt gaataaaagt
      481 cgaaaaaagg attgattatt taaatcaatc ctttttttaa ttaaattaca aagattttgt
      541 aaatttagat aataattttt taaaattttt gttaaaatca gacaaattca aaaaaatagt
      601 tgaaattgta atatttatga tttaaactta atttaatgga ataatattca tatttataca
      661 actaaagtat tttaaaaaaa tataaaataa ttaagggggt tttactaatg aaaaaaagtc
      721 tgtctttttt gctatctttt ctcattttag taagcacttt tctttttaat actcaaaaga
      781 cagaggcttt agagatgaat gaagaagcaa gagaaaggaa gtcttatgaa agatctctgt
      841 ctgtttttga gtatgtttat gaaaaaaatc cattttttga ggaagaattc tttgattttg
      901 ttatggtgca agaagccgtt tatgaaaaac caaatttagt gattactgat ctcacaacgg
      961 tccatgaaga tatacaagac tacaaattag taccttttac ggtttatata gaaaataccg
     1021 ggggaatgac cagggaagat atctacttaa cctggaaaat agatggaaaa actgttggaa
     1081 cgactgttct tgaaagaggt atgtttcctt atgaaagaag aagttctacc tttgaatgga
     1141 gagctgtgcc gggaaatcat attcttacgg ttgtggttga cgaaagagat tacatagaag
     1201 aagaggatga aggcgataat atttttgaaa aggaactata tgtaagagat gcgcttaaat
     1261 taaatttgaa cagatattct gttgaccggc cttttgtcca gactttagat actggttcag
     1321 gtttttccgt ggctattagg aaagatggaa agatagcatt tacaggaaac ggtagagaag
     1381 gtcgtagtaa tgtaagctta tgggaagata ttgtacagct ttcagcagga gctgctcata
     1441 ttgttggtct taaagcagat ggtactgctg ttgtagcagg ttacaatgca gataatctct
     1501 acaatgtagg tggttgggaa gatattgtac aagtatcggc aggatatgac catactgtgg
     1561 gtttaaaaag agatggaaca gtcatcgctg ttgggttaaa taattacaat cagtgtagtc
     1621 tggaagactg ggaagatatc gtacaaatat ctgcaggaga atcttttaca gttggactga
     1681 aagcggatgg cagtgttgta gcgaagggaa ttaataatca aggtcaatgt aatgtaggag
     1741 actgggaaga tattgtacaa gtatcggcag gcatgaatca cactgtaggt ttaaagagtg
     1801 acggaacggc aatagccgta ggaaataatc aatatggcca atgtgatgtg gaaagatggt
     1861 cagaactggt ccatgtatgg gcaggagaca aggttacgat tggtgttcga aaagatggct
     1921 ccgtattagc ttgcggaaat aatgattatg gacaaaccaa tgttagtcat tggacggata
     1981 ttgtacaagc ctttgctggc aataatttta ctttggcatt aagacaagat ggaaaagttt
     2041 tagcaacagg ggataacaga aaaggtcagt gtaattttgc tggcgtagaa tggccggatg
     2101 ccgaagatat tatacaagta tcagcaggaa caaatcatac agttttatta agaagcaatg
     2161 gtaaagtcat tgccatagga gccaataatt atggtcaatg tgatgtatca gaatgggaag
     2221 acattattca agtggcagca ggcgaagacc ataccgtagg cttaagaaga gatggaaaag
     2281 ttgtagctgt agggagaaat ttaaaaggag aatgtgatgt agaagacttg gaagatatcg
     2341 tgcaaatatc agcatcatcc tcaagtacag cggctttaag aagtgacggg aaggtttttg
     2401 tactgggtta tggtgcctca aacagaataa ataattggga agatattatt caagtttctt
     2461 caggatatct tcggacagta ggcttacaaa gaaatggcag gccagttgct gaaggataca
     2521 actattatgg gcaaaataac atagaaaact gggaagatat tattcaagtc tcagcaggtg
     2581 aaaatcatac tttaggttta agacaggatg gaaaggtttt ggcagcagga agaaattaca
     2641 acggtgagtg cagggtatcg gactgggaag atattattca agtggcagca ggtgaaaatt
     2701 attccttagg tttaagagca gacggaagag tagaacaagc aggctcaaat tttgatttat
     2761 ctgaatggga aaacattgtt caaatatcag cgggagacta tcatgccatt gctttaaaaa
     2821 gagatggaac agttataggg gcaggagata atacttatgg tcagtcgaaa gagattgcag
     2881 cagaaatatt atcagtacct gaattattca gagaagaagc cttttcatta gaagtcacag
     2941 aactgagcta ttcctttgat gaaagctata atgaagaaga ttattgcgat ggaatgaata
     3001 tgacaattca tgcagagatt atgaataaga gcagtcaatt gattgacagt acagcagtat
     3061 tattcaaatt ggatgatcac gtggtaggtt ctaagaccat caatcaattc cagccaggag
     3121 aaaaaaggac tgtaagtctt agctgtctat ttgatgccgg tgctaaaaat cttatagtca
     3181 taccagatag ccgttttccg gataatgctg ttacaacatt atcccttcct gatgtggaaa
     3241 aagcagaatt aagtttatct gtgcggatag aaaactataa caatcgcttt attgtttatg
     3301 caagtgttag caatgaggga aatacgacta taaggaatat cgatattgac tatgtattag
     3361 atggagaaaa aataggcagt tcaacaatta gaaatggcat aaaaaagggc gaaacaagaa
     3421 taagtagttt acagttttat actactgcag gaacgcacca gttaaaagtt ctggttgatc
     3481 ctcagaatat tgtcccggaa gaaaataaag aaaacaatga ttatacagaa gagattacag
     3541 cagtaatcat tcctggtgtt gagtttctca atcttgaaag aggacaaaaa ataacgagtc
     3601 catatcaaat cgaatggcaa gccgcaagcc agcattataa tccagatgaa ttaaaaattt
     3661 ctcttagttt aataaatgat aaaggagaat ttttaatcgc aaaagatata gaaaatagtg
     3721 gattttataa ctggagtttc ccggacattc ctgaggggga atacaatctt agaattattg
     3781 caacagatcc taaaggcaat gcatctgaag cttccaggct tatccgtatc atcaaccctg
     3841 ccagaataga aatcacacat aatagttaca aaatgattat tttagaaggt gatgaagcaa
     3901 agcttgattt acttgtaaaa aatcttcaac cgcaggaaga caggattatt ctgtccatag
     3961 aaaaggataa atcattggtt atggatgatg atattatttt gaccatggaa ccttgggaag
     4021 aaaggtggat tcaattggat atatcctatc cttcctgtgg tttataccag ataaatattg
     4081 tggctgagtc agaaaacatt ccttcaataa ggtgtgaaga aacggtagaa ttagcagtat
     4141 tgccacctgt tttgcagtca ttttttgaac atttaagaag attttttgcc gaaatgaatc
     4201 tactttaaag agaataatta attttttgaa tgtaataacc tagataataa aaaaggcagt
     4261 ttcatggaaa actatagaaa ctgccttttt cttttacaag taaagatatt caaataaatc
     4321 tgttctcgac aatttataac ttactcatta taatgtaggt aatacaggtg ctaaatgatg
     4381 actctaaata aatagggagg aaaatatgga aaaaataact tataccgaca taaaaaataa
     4441 tccagaaatc aatacttata ttaaaaaagg cgatgaactc ttagaaatca tgggatatac
     4501 ggatcattcc gaaattcatg ctgcaaaagt tgctgaaaca gcagcagaaa tccttcgaaa
     4561 actgggttat tctgaaagag agtgtgaatt ggcaaaaatt gcaggctatc tccatgacat
     4621 tggcaacgct gtcaatagag tagatcatgc acaaaccggg gcagtcatgg catttaacat
     4681 tctttccaag ttaaatatgg atcctgaaga gattgcgacc atcgtggcag ccataggaaa
     4741 tcatgatgaa tctacaggga cagctgtgag tccggtttct gccgctttga ttttagcaga
     4801 caagaccgat gtaagaagaa gccgggtaag gaataaagaa ctggttactt ttgatattca
     4861 tgacagggtc aattatgctg tagaaaaatc aaaagttata atcgatgaag aaaaaagaaa
     4921 gcttatctta gagctcacta ttgacatgag aatttcatct cttatagaat actttgaaat
     4981 ttttcttacc agaatgctga tgtgtagaag agctgctgac tttttaaata taaattttga
     5041 attagtggcc aatgaaaaga aaatggtata gttaaaaata ttggatcata agaaagctaa
     5101 tcccaacaga ggccaggacg gataaaatga tattgccttt ccaccaggac aggaatactg
     5161 cagtaacaat gccagcccag actgcttttg gctgattggg tatagcgtta aagacaccag
     5221 gcagtattaa agcccctaaa gttgcatagg gaatataatt aagaaagcgt tttaaggttg
     5281 gatgaatctc ttttttggat ataaaaataa agggaagcag tctgggaata aaagtaacaa
     5341 cagccatgcc caggatgata aaaaaataag atttcatgga ttaacgcctc cttcttcttt
     5401 gggtaaaatc attaaaccaa ttaaagcagc tagggttata gacaggatta aattccagtc
     5461 ctcaggaatc agatggctta tatttaaaaa tgcgtggatt cctcctgaca gtaaagctaa
     5521 aaaaatgact tcttttgact ttttcatgga gggaaccagg atagcaataa acatagcata
     5581 cagggctatt tgcatactgc tttggatggc ttctggcaga acgcttccca gtaaatagcc
     5641 tgcgacactg ccaagaaccc atgaaaaata agcagttgta ttgactgtaa gggtataaaa
     5701 aaaagacaat tctttgtttt ttgtagctaa aactgaaaaa gtttcatcgg ttataccaaa
     5761 agccacaaga ggaataagtt tttttcttgg gcttttaatt ttatcagcca gggaggcact
     5821 catcaaaaaa tgtcttaaat tcatgactgc ggtggtaaga ataatttgaa aagcaggtac
     5881 agttgcagca attaaattaa cagccataaa ttgacttgct ccagcaaaaa cgaataaaga
     5941 aaataaaaaa cagtcccata agctaatttg atttgctttt gaaataatac caaaagccag
     6001 agcaatagga atgtatccaa tagcaattgg aagagcatct ttaaatcctg ataagacttc
     6061 gccattataa tatttattca agtgatccac gtccttttaa gctttttata tcgcatttta
     6121 tcattttgga tttaatgact gcttattgtt aaaataaata ttatgaagta ttatataata
     6181 taaagatgaa atttaaaaga ataattcgaa tcattaagaa agagggttta aaatgagtca
     6241 agcggataag atttttattg aaatgagcga aagtattttg aatgacggtt ttagttcaga
     6301 gggtcaagat gtaagaccca gatgggaaga tgggacacct gctcatacga ttaaaaaatt
     6361 tggagtcgtt aaccgataca atctggctga ggaatttccc attctcactc ttagaaaagt
     6421 accttttaag agagcaattg atgaaatact ttggatatgg cagaaaaaat caaacaatat
     6481 aaaggattta aacagtcata tttgggatgc ctgggctgat gaaaatggtt ctattgggaa
     6541 agcttatggg tatcagcttg gaattaaaca taaatacaaa gaaggggaat ttgaccaggt
     6601 tgaccgggtt ttatatgatt taaagcataa tccatatagc aggagaatca ttacaaatct
     6661 atacaatcac caggaccttc atgaaatgaa cttataccct tgtgcttata gtgttacttt
     6721 taatgtgaca gggaataaat taaatgccat tcttaatcaa aggtctcagg atgttttagt
     6781 agcaaatgcc tggaatgtat gtcagtatgc tgtcttaata catatgtttg cccaggtgag
     6841 caatttagaa gtcggggaac tggttcatgt tattgcagat gcccatattt atgacaggca
     6901 tgtccctatt gtaaaagaat taataaaaag agaatcttat cctgctccta agttcataat
     6961 aaataaagaa atcaagaatt tttatgattt taccgttgat gattttgaac tggaaggcta
     7021 ccaacatgga gagccaatca agaatattcc tgtggcggtt taggaggata ttatgaatat
     7081 tattgtatca gttgaccaaa attgggctat tggttataag ggagatttac ttcaaaaagt
     7141 tccggaagat atgaaacagt ttaaagaaaa gacctgggga aaggttgttg taatgggaag
     7201 actgaccttt gaatcattac ccaatcaaaa gcctttacct aacagaacca atatcattct
     7261 taccagaaat gaaaacttta gggtagaaga tgccattgta tgtcattctg tggatgaaat
     7321 acttaaaaca actaagtatt gtaacccgga ggacattttt atcataggtg gagaacatat
     7381 ttataaaact tttcttccct attgttcaaa ggcttatatc acaaaattcc ataaagtata
     7441 tccagcagac acctttttcc cgaatcttga cgaggatgaa aattggcgac tcattgaaaa
     7501 aagcgggccc agacaatatc aggatcttac ttttgaatac attacctatg aaaatataaa
     7561 taagatttaa agtgtaacaa aagaatttca gattcttttg ttatttattc atatgatttc
     7621 aagaatatta tttgaataaa atacttttta aatttttatg atataatatt ctatatctta
     7681 ttcaatcgaa ggggatattt atgttaaaga aatttcaata taacgcacca gtcattttaa
     7741 cctttagtct aatttctttc ttagttttgc tgttatctta ttttactggt ggacttacca
     7801 atcagttatt attttgtgtt tatagatctt ccttaaagga ccctttgttt tatttaagat
     7861 tatttaccca tgttttggga catgtcaatt tgcaacattt tgttaataac tttattttaa
     7921 tcttattgat aggaccaatg atggaagaaa agtacggttc gaaaaaaatg ctttttatga
     7981 ttatatttac ggcttttgtt acgggtatta ttcatattct gttctataac agtgctttat
     8041 taggggccag tggtatcgta tttatgctta tattattgag ttcttttgca aatattcaaa
     8101 aaggaaggat tcctttaacc tttgtattgg tagtgattat ttttattggg caggaactgg
     8161 ttaatgctat tatgctgcat gatcagattt cacaattaac ccacatcata ggaggtattt
     8221 gtgggggatt ttttggttat aaaattaata actctaagaa agaaagttaa gcatgttttt
     8281 tatcagagta tactataata tcaaaaggaa caaagaaata atattaataa gcatatcaat
     8341 ttattctttc atttttttat taattaagta acaaggagat ggttttaaat ggaaaacaac
     8401 aaagaaatga cgatggcaga aatgatagag gacatcgata agtcaatgag aaaattaaat
     8461 aaaggggaca ttattaaagg aaaagtaata aaagttaatg aaaatgatgt cttagttaac
     8521 ataggctata tgctggatgg aataattcca aaagaagaat tgtctgatga tgccaatata
     8581 aatccaaggg atattgtaag tgtggatgat gagatagatg tctatgtatt agaagttgat
     8641 gatgatgaag ggaacgttcg tctttcaaag aaattagcgg atcaaatcgt ggtatgggat
     8701 gaattggaag aatactttaa gtcaggcagc ccattagaag ttaccgttaa ggaagttgtt
     8761 aagggaggcg ttgttgcaaa tattaaaggc gtaagagcct ttattcctgc ttctcagctt
     8821 tctgttcatt atgtagaaga tttaaatcgt tatcttggaa aggccttaaa agttaaatta
     8881 attgatttcg acagaaaaag ccgcagagta gttctttctg ctaaagaaat cgagcaggca
     8941 gaaagggaaa tcagaaagaa agaaatctgg aaaagtctaa aaagaggtga aaaaagaaaa
     9001 ggtgttgtta ccagattggt taaatttggc gcttttgttg atataggagg tgttgaaggg
     9061 ctcattcatt tgtctgattt atcctggaaa agagtcatgg atccttcaga agtggtgtct
     9121 gttggagatg aagtagaagt atatgtttta aactttgatg aaaagaaaga aagaatttct
     9181 ttgagtttaa aagataaagc agaagatcca tggaacaata ttatggataa tttcaaggtc
     9241 aatgatctgg tagaaggtaa agttgtcaga cttgcacctt ttggggcttt tgttgaaatt
     9301 gcacctggca tagaaggttt acttcatatt tctgagattt ctgataagcg tatttcaaaa
     9361 gtttcagaag ttctttcaga aggggataag gttaaagtta gaatcataga attaaaacca
     9421 gataataaaa aattaagttt aagtatgaaa gaagcccatg aggattattc tgaagaaatt
     9481 aaaaaataca attttactca ggacgaagat gttacaattg gtgatctcct taaagaccag
     9541 ttaaaaggat taaaatttta gataaaagca taacaacccc ggctattacc ggggttgttt
     9601 gctttgaatt attgtaaggt ttgtaaatag atttgttcaa tttctttttt aaatggagca
     9661 aaagattttt ctgaaaactg tcttccttcc ttacttgtat aaagaagaag agtgccgtca
     9721 tctctcaggt agaagatact tgcaattttt gtatttggat tataaataaa atagtaattg
     9781 ttaactaatt tttgagaaag gatggtttct ttatccagcc tataaagatg aagatattcc
     9841 agaggaatct gactttcatc aggtatatca acgggtatat ctgaaaggac taggtcactt
     9901 tctacaattg ccttaaccat ttcatccata gaagcggtaa ttttatcgtc agtacctctt
     9961 atatggttga tgcctgcaat tgcaaataat ataataaata agcctacaaa aagccaaata
    10021 aaacggttgc cattgttttc attcatatta gatacctcca taatgataag catttctaaa
    10081 attataacag tttttacctt caaattcaat tgttcacgtg atttttctaa aataattaaa
    10141 gagacacttg aaatccatgg caaatattgg taatatatta attaatttac attatttgcc
    10201 aatggaggat ttagcatgaa agtattaaaa gtatttttta aattactttt agttatttta
    10261 atctcatttt ttatttctat gcatatttgg gcccaggaac ccggacaaga agataataaa
    10321 ggacagtcag caattagcct ggaagaatgc attaaagaaa tgatgaatat taaaaatgaa
    10381 aaattagaca ataatcaaaa gagttatctt gaggcaggag aagaaagggg ttatcttaaa
    10441 ggaattatca acagaaataa agatgaggct gtaacaaggg caatgctttc aagaattata
    10501 gttaattttt tagatattaa gcccatttat tcgaaagatg acctttttga agatttaaaa
    10561 gcttttcctg atgcagaata cattaaaggg gtttatgcag aatatttgat agatggtata
    10621 gatgaaaagc atttttatcc taatcagcct gtttctaaaa atgaatttaa gactatatta
    10681 gaaagaataa aaaaatatta tcaaaatcca aataatttta aagcagaaat gaaaagccag
    10741 tccagcccca aaaaagaaga aaaaataaaa aaactcttgt ctatgaccag ctataaaccg
    10801 ccaaaagaaa aggttgctcc tggcaccagc ctgggtgtat tagaaaaggg aattgcacaa
    10861 gcttttatta atgactacta tataccaagt ttttcatacg acaatgcttc tatgataatt
    10921 ctttctgatt taaattatta tggctttgat attatttggg atgagaccaa taagaagatt
    10981 tctattattg aaaataaaaa taaagccaga tcagccctta gcgaagaaga aataaaaaaa
    11041 cgtgaaaaaa attatattgg aaaacccctt ctttatacta atatagctgt atatattggt
    11101 aacagaaaga ttccctgttt tttagcagac aattcaacca ttatctatat taatgattta
    11161 agtattttcg ggaatttaaa ttgggatggc caaaataaaa ttataacgct gcagacaaga
    11221 gatacggata tcaacgatga tttgggcgta gaactggtca atcaaaaagt cattaattat
    11281 aatgaagaaa ccattgattt aaggatgacg catctatggt atgatgaaaa agtaaagaag
    11341 gtacatactg ttgaagatag tgtttatggg attccttccg gtgatgcgat agaatttagc
    11401 ttagaaaaat atgatttata tgaaaaaagt tattatttag gtacggctct taaaagtaca
    11461 aacaaaaaag ataatccttt ttacagccgg gaagtaaact acctgcaatc tcaaaagatg
    11521 atagattata ccagcaagct ttataaggac ccggaagaag aactcttatc tctggtaaag
    11581 gcttcaatta ttataggaac catgaagaag aatgccggac cttttgcaaa ggatgaaaag
    11641 gttgaaatct tatatgctga agcaggttcc tggtatcagt gtcgcagcat cagtacaaaa
    11701 aaagaagggc gtgttccctg gggctctgtt tccataccac cagaccctcc taccaataca
    11761 aaacgcctga caaaagaaca gcttgaagcc tatgtaaagt taaaaggctt tacaagcaat
    11821 accaattatt ttgtatggac cgatttggac aggcaaatga cgtatgtctt taaaaaggaa
    11881 aacaacgaat ggaaacttat ccgcagcatg ctttgttcta cgggaaaaaa tataaccccg
    11941 acaccaagag gatttttcac tataaaagag cgcggagcct attttggcaa gggttatatg
    12001 tgtaagaact gggttcagat atggggggat tatttatatc attctgttat catggatccg
    12061 actggcagtt atgtcctgga aggcaacgtt ttaggtaaaa gggcttctca gggctgcatc
    12121 cgcttttctc ttgaggacag caaatggttt tatgatacta ttccaagaaa cactactgta
    12181 tggattaact aatctttttt tgccaatttt aataatcttt tagaaatctt gattttttca
    12241 taaatctctt tataatcatg agcattgagt tcatcaatat aatttttaat atagctttta
    12301 gaactgattc catggcttac gacaaaatcg acagctttgt tataagccac tgctgcatta
    12361 ataggagagg aatagcaacc aatggtccag gtcccgtaaa tattgatgcg ggcgacaaag
    12421 accatccgtc ctctcctata ttctctgcgg acgccgtaat aggggttaat tacttcaata
    12481 ttgtagtgtc tgaagtcgta ctcatcccca tttttaaaac ggtagtcaat gccttttaca
    12541 gcaaagtttc tgataccgta gcgggttaag atattttgct ggataccaaa gtcattgaca
    12601 taaaaatagc cgcctttttg gtggatttta tgagaagcat agtagaataa atcatcggta
    12661 tcaaacttaa gctcaatatc agggcttaaa aaataggaaa aataatactt atgaaggtaa
    12721 atggggttac caaaataata accgttatca cggtaattac aaagaataac ccacttttcg
    12781 aaagaaagaa tcttactttt attataatcc tccagcttat aacgcttttt gaataatatt
    12841 tcacaggctt ctaaataggc tttatgagcg agcctggagt tatcaaaact tcccaggctg
    12901 atatgtttgt ttttataggt gatggatgag cggaaatagg cttctccatt ttttttatat
    12961 gctttgaaaa cacctttcat aggattcccc ccaggcataa aggcaaataa ctaataccat
    13021 aataacatag ggagaggaat caataaagag ttataagaaa taatctggtt attctgcact
    13081 gctgagaaac gcacttgaca cgaacgtctg ttttgaatta ctcttataag taagagaaaa
    13141 atagggagtt gactatgcgg atggggaaaa agaaggatac atatggtaat gacagcattt
    13201 ctcttttaaa gggagcagac agggtaagac taagaccagc tgttattttt ggttccgatg
    13261 ggcttaaagg ctgtcagcat gctttcattg aaatattggc caatgccatt gacgaggcca
    13321 gagaaggttt tggtaagaaa attgaagtca cggtatttaa agattattcc attatggtta
    13381 aggattatgg ccggggtatc cctttggatt ataacgaaaa agaaggccgc tataattggg
    13441 agttggtttt ttgcgaactc tatgccggtg gtaaatatga aaacaataca ggaagcaact
    13501 atgaatatag tttaggttta aacggtctgg gttgctgtgc tacacaatac agttctgaat
    13561 atatggatgt tactgtatac agggacggct ataagtacga attgcatttt gaaaaaggaa
    13621 ataatgtcgg aggcatgata aaagaaaaat ttaattatga gtctacaggc actattatta
    13681 agtggagacc ggatttagaa gtttttacgg atattaaaat tcctcttgaa ttttttaaag
    13741 aaaccttaaa aaaacaggca gtggtaaatg ccggagtttt atttgtctta aacgatgaag
    13801 cttctggtga aagttttgag tattgttatc aaaacggaat tatagattac attagtgaaa
    13861 ttaacagtga caaaggtttt acttccattc agttttatga acacgaaacc agtggaagag
    13921 acagggaaga caagccggaa tataaagtaa aatttcagat ggcatttgtt tttaataatg
    13981 aaattaactt attggaatac tatcataatt ccagcttctt agaatatggc ggatcacctg
    14041 acaaggctgt aagaagtgct tttgtctatg ccattgacca atatttaaaa gctgaaaaga
    14101 aatataataa aaatgaaaag aaaattacct ttgcggatgt tgaagacagc ttaattttag
    14161 tcagtaattc tttttctacg attaccagct atgaaaacca aactaaaaag gcaattacca
    14221 atatttttat acaggaagcc atgacggctt ttttgaaaaa gcagctggaa atttatttta
    14281 tagaaaataa agaggaagca gaaaaaattg caaagcaagt tctgatcaat aaaagaagcc
    14341 gggaaaaagc tgaaaaaacc cgtattgaca ttaaaaagca attaggcggc aaagtggatg
    14401 tggtcaacag ggttaaaaaa tttgtagatt gcaggacaaa ggatgtttct aaaagagaac
    14461 tgtatatcgt agaaggggat tctgccctgg gttcttgtaa gctaggaaga aatgcagaat
    14521 tccaggccat tatgcctgtc agaggtaaaa ttttaaactg tttaaaagct gatgaccaca
    14581 aaatattcca aagcgaagtt atcacagacc ttttaaaagt attaggctgt ggagttgaat
    14641 ttagcaacaa gtacaacagg gatctttcaa attttgattt aaataaatta aattgggata
    14701 aaattattat ttgtacggat gcagacgtgg atggttacca aatccgaact ttgatattaa
    14761 ccatgcttta caggcttgta cccaccttaa tcagggaagg gaaagtctat attgcggaaa
    14821 ccccgctata tgaaatccgg accaacaaaa agacttattt tgcttacaat gaggaagaga
    14881 aaaaagccat tttatctaga attaaaggtc attacactat catgcgttca aaaggtttag
    14941 gggaaaacga cccggatatg atgtgggaaa caacgatgaa cccggaaacc agaagactga
    15001 ttaaaattgt cccggacgat atagaaaaaa caaaagaaat ttttgaaatt cttttaggag
    15061 acaatttaga aggcagaaag gaatatatat cagaatacgg tcatctatat ttagatccta
    15121 cggatcttgc ataaactttg attaaaggag taaagacatg ggggaaaaaa cgacgactat
    15181 tgcagaaaag attacagata ccttaaagaa gaactatatg ccctatgcga tgagtgtgat
    15241 tgtttcccgg gcaattcctg aaattgatgg ttttaaacct gcacacagaa aattacttta
    15301 tactatgtat aaaatgggtc tgctgaaagg aggcaggacc aagtcggcca atatcgtagg
    15361 tcagaccatg cgtcttaatc cccatggtga tcaggctatc tatgaaacca tggtcagact
    15421 caccacaggc tgtgaatctt tattggttcc ctttattgat tctaaaggta acttcgggaa
    15481 acaatattca agagatatgg cctatgcagc gcccagatat acagaggcta agctggaaga
    15541 aatctgtgaa gaattattta aggatatcga taaagataca gtagatttcg tagacaacta
    15601 tgatggcaca atgaaagaac ctcttctttt accagcgacc tttcctaata ttattgtcaa
    15661 tcccaaccag ggaattgctg taggtatggc caataatatt tgttcattta atttaaaaga
    15721 agtctgtcag gctaccattg cttatttaga agatgaaaag gtggatattt ctaaatatat
    15781 caaggctcct gattttccga caggaggcaa tatcatatac aatgcagaag aaatgaaaga
    15841 aatctttgaa acgggcaggg gtagtttcag aatcagagcg gtttataatt acgataaaaa
    15901 aagcaatgcc attgaaatca cagaaattcc ttataccact accgtagaag ccattattga
    15961 aaagttagtc agcttaatta aggcaggaaa aataagagaa atcaatgata tcagggatga
    16021 aacagattta ggtggtttaa aaataacttt agacttgaaa agaagtactg acccggatga
    16081 attaatgcgt aaattatata gcttaacacc tcttgaagac agttttaatt gtaattttaa
    16141 catcctggtt gatggtcatc caagagttat gggcatcaag gaaattcttt atgagtggac
    16201 aaaattcaga atcaactgta tccgcagaag gacacaatat gatatcaata aaaagtcaga
    16261 aaaaagacat ttactgcttg gccttgaaaa aattctgctg gatatagata aagccataaa
    16321 aatcatcagg gaaacggaaa aagaaaaaga agtcattgca aacttaatga aaggctttaa
    16381 tattgatgaa gtccaggcag aatatgtagc agaaatcaag cttagaaatt tgaataaaga
    16441 atatatttta aaccggacca aagaaattga aagtatcgat aaagaattaa aagcattaaa
    16501 tgtcttacta aaagatgaga aaaaaattaa gaaagtgatt gcccaggaac ttgaaatggt
    16561 tgcaaaaaaa tatggaagac cacgaaaaag taaaatcatt tcagaagaaa aaattgaaac
    16621 cctttcaaaa gaagatttca tagacgatta caatgttaaa attttcttaa ccagacagca
    16681 gtatattaaa aagattagtc tagtctccct tagggcttat tctacccagg aattaaaaga
    16741 aggcgatgaa attattcagg aaatagaagc aacaaacaag tcagaactct tattgttttc
    16801 agacaaacag aacgtttata agataaaagc ctatgaatta gaagacacaa aagccagcag
    16861 tcttggagat tttatcccca atattgtaga attggaagaa ggcgaaaaca ttatttttgt
    16921 tgctgctgcc agtgataagt atgaagggga tttactcttt gcctttgaaa atggcaaact
    16981 cgtaaaaatt ccttttaggc tttatgaaac caagcaaaac agaagaaaac ttattaattg
    17041 tttttatggc cgttcaaaat gtgttgccat tttgcatatt acagaagata ttgatatttt
    17101 gttaaaaagg agcgataata aagttgccct tatcaatacc agcttagttg taaaaggcaa
    17161 taataaaaaa gcggctggca atcaggctgt caaagtaacc aaaaaaagcc gtcttgaagc
    17221 agtattgaca gtagaggatt tttcaggtaa tgacgtggag caataccggg caacttccat
    17281 tccggcaaaa gtacagtcca tttatacgca aatagaactt aatatataga aaggtactga
    17341 tttcaatgaa aaacgatatc aaagaaatat tgtttagcga agaggaaatt gcaaaaaagg
    17401 taaaagagct tgcgcaaaaa attactgaag attacagagg aaaagatctt accgttattg
    17461 gaatcctgaa aggttctaat attttcatgg gagacttaat ccgaaaaatt gatattcccc
    17521 ttcaaattga ttttatggtg gtatccagct atggtcagtc cactgaaagc agtggaattg
    17581 ttaaagtctt aaaagactta gattacagta tcgaaggaaa aaacatctta attgtagaag
    17641 atattatcga tacaggtttg accctgcatt acttaaaagg aatcttacaa aaaagaaatc
    17701 caaactccat taagatttgt accctattag ataaacctgc aagaagaaaa gtggatttag
    17761 aggtagacta tgtaggtttt cagatacctg atgaattcat tgtgggttat gggattgact
    17821 atagtgaaaa atacagaaat ttgccatttg tagctgtgtt aaaagatgaa gtatataaaa
    17881 agtaaagtga ctttgggctg tcttaaagaa tttaagacag cccttaatat ttttaagatg
    17941 ataaaaaggg caataaaaat gattggttga aataatgata aattgatatt agtataatga
    18001 atgtaaaaaa gaaggataag agtgaaatgt aaaacagctg gcagtaaggg agcatatagt
    18061 aagcttaaaa aaattaaatc gtatagatag agagaagcaa cttgtctgat taatcaggag
    18121 agagaaaaaa tattaattaa aatattctaa tgattgcaaa cttgatataa ttatgatatc
    18181 ataatgatat aattaatata taagttcata ataatataat agacaaaaat ataaggaggg
    18241 aatgttatgg aagaaaaaat tttaaaagca aaagcgggaa tgccttgttt aattcttttt
    18301 attttcctgt atctggctgc gattgcttct attattttcg gaggaaacct tttaagcaag
    18361 cagaataatt tgggcgcagt ttttttaacg atagggatta tttgggtatc gattggtttt
    18421 gttccttttc ttggtctgaa aattattaag ccccaggaag ctttagtact taccttgttt
    18481 gggaattata taggtacttt aaaagaagca ggtttctttt ttgttaatcc ttttgcagtt
    18541 gctgttaacc cagctgccaa aacccgtctt ggacaaagtg atgatgtcag ccatattccc
    18601 agtataaaat tagaagcggg aataaacctg caaaataatt atgtaagtaa aaaaatttct
    18661 ctgaaagtaa tgaccttaaa caacaaaaaa caaaagatca atgatgcttt gggtaatcct
    18721 attgaaatag gtgttgcagt aatctggcgg gtagtggata cagcgaaggc agtttttaat
    18781 gtggataatt ataaagaata tctttcatta caatgcgatg gggcattaag ggaagctgtc
    18841 cgtatgtatc cttatgacat tggtttggtt gaggataatg gcgctaacgg ggaagctgat
    18901 gaatttagcc ttcgtggttc cagtaaggaa gtggcacaac ggattcgtga gacgatacag
    18961 gctaaagtcg aaaatgcagg cattgagatt ttagaagccc gcattaccca tctggcttat
    19021 gcacctgaaa ttgctgcagc gatgctccaa agacaacagg caagagctat tattgatgcc
    19081 agaaagatga ttgtggacgg tgccgttggt atggtagaaa tggctttgga gatgcttaat
    19141 gaaaaacaag ttatacaact cgatgaggaa cgtaaagctg ccatggtatc gaacctgtta
    19201 gttgttcttt gcggcaacag agacgcccag ccagttatca acagtggcag tctgtactaa
    19261 aatggataat aaaaagcaaa ttcctttacg aatatcttca aagttatatg aagagatagc
    19321 tgcctgggct gaagaagaat tccgttcggt aaatggtcag attgaatatc ttcttacaga
    19381 atgtgtgaag caaagaaaaa agaatggccg gcctgttccg aaagatcttg gaaaaaaaga
    19441 agaataaaag caaagtgtac acaggtggtg ggttatttat aaatttgctt acctgtgtat
    19501 tttatttttg aatagaaata aaatgctgtt catttttaat gttttaaatt gtatacaagg
    19561 agaaggaata gaagacgtag cttattcaac agcattgtgt atagtttaaa atgttgaaat
    19621 agtgagaaat ttatgttata ttaaatttat tgtattaggt gtaaaaagga ggagaatgat
    19681 gcagataaat acaattaaat aagtgctcag acaattcatt tgttatgcaa gtattggtag
    19741 tttggtattc tttacaggat ggcagccttg ttatgcagaa gacagcagct atttagaaaa
    19801 tagagtaata gaaatttcag ctggtgctta tcatacagtt gccttgaaag aagatggaac
    19861 ggtatggaca tggggtaatc aaaattttga atctacagat gattatgacg aggagcaaag
    19921 ctgtaatagt aaggcagaac aagtagaaag tctggaagat ataaaagcca ttgccgcagg
    19981 atatcatcat gttgtagcct taaaagaaga tgggaccgta tggtcttggg gaaataatgt
    20041 ctttggtcag cttggccata cagattttgt agtaagaaat aatcaaccaa tgatggtagt
    20101 ggatctgaaa gacgtgaaag ccattgccgc aggggaatgg catacggtag ccttaaaaga
    20161 agatggaagt atatgggcat ggggtgataa tgaagatggt cagcttggtg atggccgagt
    20221 tatagctaca aacaaaccag ttcaagcaat agatatagaa gaagtagtag cgatagcatc
    20281 aggtaactgc tttacagtcg ccttaaaaca agatggcacc gtatggacat ggggacttta
    20341 tttttactat gatccgatag aaaatgtaga tatagcaatg acatctactc agccggtctg
    20401 taaagaaggc atagaagatg ttaaaacaat agcagcaggt tctgaccata ctatagccat
    20461 aaaagaagat ggcaccgtat ggggatgggg atctaatttc gctggtcaat taggtgctat
    20521 tgtgatagcg gacagtgata caaataatga attcaaatat gaacgattta tgcaagtaag
    20581 tggtatggaa aaagtcaaag caattgctgc aggatacaat tatacaatag cgttaaagaa
    20641 ggatggcagc ttatgggtct ggggcagaaa taatctgggt aatttaggag atgattcaat
    20701 agaaacaatt agcattgagc cagtacctgt aaaggatttg gaagatgtca tagcagtgtc
    20761 agcggctgat gatcatatag tagccttaaa aaaggacggt accgtatggt catgggggct
    20821 taatctgtac ggccagttgg gagatggtac ggtaatgtat actagtaaac cagtacaaac
    20881 aaaaataaaa taaaaattac ttttataaaa aggttttgtt agtaagacgg tatctattta
    20941 tggataccgc cttttatgtt aaaaaaaagt agcgtataat ggtaggagaa ttatttgaaa
    21001 tatataggat aaggattatg ataacaagat agtaatgaaa tatataaaaa gacagggaga
    21061 atgttaaatg agagtatttg tcgcgattga ctttgataat aaattaaaag cctatttaaa
    21121 agaaaagcaa aatgagctta agacatattg cactaaaggg aattttactc atgaagaaaa
    21181 ttttcattta acaattgttt ttataggaga agttaaacca gaacaaattc ctgagattga
    21241 aaaagtaatg gataatgcgg caggtcaaag tgaaggtttt acccttaaac tgagtcattt
    21301 tgattatttt aaaagacagg atgaatacct gccctggatt ggtttagacg gggatttaac
    21361 aagtttaaat gatttatatc gtaagcttca ttcgaaatta aaagttcttg gtttttcatt
    21421 agaagaaaga gatctgaaag ctcatatcac tttaggcaga agggttaaat ttaatgagcc
    21481 tgttcaaatc attaagaaaa attttatcat tgatcccata accgttcccg taacttctat
    21541 tgtccttatg gaaagtagca gagtaaacaa taagttgact tatatacctc tttatgaaaa
    21601 aaatttaaat ataagaagtt aactattata ataatataag catagtaata agctttttta
    21661 tgttactata ttaatcaaaa ctttaaaaaa caataaactt ttaaattagg aaggaatgag
    21721 agcatatgga taaaaaccca gtcgtaatga tttgtatgga agatggaagt caaatgaaag
    21781 ttgaactata tccggatata gcacctaata cggttaataa ttttatttct ttagtcaaga
    21841 aaggttttta tgatggctta atttttcaca gagtcatcaa aggttttatg attcagggag
    21901 gagatccgca aggaacagga gcaggcggac caggatatag catcagggga gaatttataa
    21961 taaacggctt tgataataat ttaaaacata ccagaggggt tatttctatg gccagggctc
    22021 aaaatccgga ctctgcaggt tcccagtttt ttattatgca tgaagacgca cctcatctgg
    22081 acggacatta tgcggctttt ggaaaagtga tagaaggtat tactgtcgta gacaagattg
    22141 cgagtgtaga gacggattat tttgaccgac ctttagaaga acaaagaatt aagtttatga
    22201 cagtagatac ttttgggaaa gagtatccgg aaccagaaaa attataaaaa attgagcgtt
    22261 caaaggaagg attatcctgt tgtgagtagt ccctcagttt ttctttaaaa atttacatta
    22321 aaaagctccc aaaatggcaa taatatataa aaaagatttt gggagtggat attcttgagc
    22381 agctatgata atgcaaaggc aatgagggaa cttagaaaaa gaagaaaaga aaaaaagcta
    22441 tgtacccgtt gtggcaaacc tgtttttggt gttcatgtcc agtgtgatgc ctgtagggaa
    22501 tatagcagga tttatgcctt gctgcatcca aaagaaaaag taattataag acatttaaaa
    22561 agttgggaaa taaaaaataa aaaactttat gatattctta ttaaaaagcg aattacaatt
    22621 ccacaattgg caaaaatgat aggggtttca tcaagaagtg tggacagatg ggtatttgag
    22681 ggctgtattc caaaactgga aaacagggaa aaggtcaatg cttatttaaa tatggaagtt
    22741 tttgagttag aataaaatat tttagttttt ttatattttt tctcttaatt ttttaaaata
    22801 aatgatataa tggtttaata tatcatttat tacggaggat aaatatgtgt ttaaatacaa
    22861 ttttatttga tttggatggt actttgcttc cgctggatat ggatactttt atgaatgttt
    22921 attttgaaga agtgggaaag ctcttcaggg atatagtaaa tcctaaagaa ttaattacta
    22981 atatctggaa agctacagaa gagatgatga gaaatacaga atataagacg aatgaagaag
    23041 tttttacaga agcttttgaa aaatatatgg gaagggatat ccgcattttt ataaacagac
    23101 tcaatgattt ttatgataaa gaatttatga gagttagaaa agccacaggt gattcaccct
    23161 ttataagaaa agcagtttat cttttaaaag ataagggtta tgatattgtg gtagctacca
    23221 atccgctctt tcctaaaaaa gccattgtac acaggatcag gtgggcaggt tttttgcctg
    23281 aagattttag atatatcacc tgttatgaag aaagtcatta ttgtaaacca aatataaagt
    23341 tttatgaaga agtccttgaa aagattaata aaagtcccaa agaatgtatg atggttggta
    23401 atgatgtgaa agaagattta gtagcaggca aattaggtat taaaactttt ttaattgaag
    23461 attatattgt tgacaaaaaa gaatgtgatt tcttaccaac ctatagaggg agatatgaag
    23521 atttttataa attcgtatat gaattaccag aaatatcata aaaatacaat tatctatttt
    23581 atacatattt tcgacgttaa tctggttttt cttgtgcagc tatcctataa atggtttaat
    23641 aaaatgaatg ttttttagtc aaaataatag tgtaatgaac acatcccata aaaaataaaa
    23701 atatttttca taaacaccgt tgacagcggt aaaaatgtat ggtaacttag acata
//
LOCUS       DAOSUD010000028        23732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1396_length_23732_cov_24.342146, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000028 DAOSUD010000000
VERSION     DAOSUD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 23732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23732
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_23732_cov_24.342146"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..1027
                     /locus_tag="PLG49_05250"
     CDS             374..1027
                     /locus_tag="PLG49_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutH/Sau3AI family endonuclease"
                     /protein_id="HOQ16763.1"
                     /translation="MRLEEANKRLNNLVGIPFEILFSQNDLNMIIKNKGKTGQLLELA
                     LGMNLSSSNLDFDDGELKTNKCDAAGNPKETIFITQISSIIDELIDKKPFEQTHLYKK
                     IKNILYVPVCKEGNPSDWRFLPSIHIDLSSPRYSELLNIWKQDYYSICEQIRNHIEKS
                     PDGFIHTSSGKHIQIRSKDSKPYHPIYSDIYKRNISDKNYAFYFKKQFVIDIRSMNT"
     gene            complement(1259..1537)
                     /locus_tag="PLG49_05255"
     CDS             complement(1259..1537)
                     /locus_tag="PLG49_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16764.1"
                     /translation="MLTFRDEKKDRFYFKNLDRIHEREIFLKAKSILENDMNTKFINY
                     IEIVELDIWEGKYKDVDFQLCYDIDYGPFLICKNEDILEQLEKLINKE"
     gene            complement(2126..3244)
                     /locus_tag="PLG49_05260"
     CDS             complement(2126..3244)
                     /locus_tag="PLG49_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOQ16765.1"
                     /translation="MKSILNFIKKELVLCISLLLALVSMTFVPLDKQYISYIDFHTLI
                     ILFSLMLIMAGLRSLGIFSMTGGWMLQKASNVRTLSLVLVTLCFVFSMLITNDVALIA
                     FVPFTIDVLLMAGLENYLIQTIVLQTIASNLGSMLTPIGNPQNLYLFSLSGMKLSSFI
                     SLMLPYTLLSLIALLVACVILIDKKSVSVKVQSKLNLTEAEKKKAFVYFLLFLFALTS
                     VTRIISAYIAGLVILVVVFLLDRKVLGKADYSLLFTFVFLFIFIGNIKRIPEINHWLQ
                     SIIHGHELLTSVFASQVISNVPAAILLSAFTDNISTLIIGTNLGGLGTIIASMASLIS
                     FKYYGTVEGKHNAVYLLVFTLLNLIFLGILLILYYLIH"
     gene            complement(3686..4078)
                     /locus_tag="PLG49_05265"
     CDS             complement(3686..4078)
                     /locus_tag="PLG49_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ16766.1"
                     /translation="MEEVYEFLKKCGTYYLATVEGDQPRVRPFGTVDIFEGKLYIQTG
                     KVKDVSRQIQKNPKVEICAWGDGKWLRLAGKLVRDDRVEAKKHLLDNYPELQSLYSPE
                     DDNTEVLYFEDATATFYSFTEESKVIKF"
     gene            complement(4197..5336)
                     /locus_tag="PLG49_05270"
     CDS             complement(4197..5336)
                     /locus_tag="PLG49_05270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ16767.1"
                     /translation="MVQTENIIFNIYSIVIIFIIYFFASKFFKRDSMADKLYMQILNV
                     TVILLTVDILSRFDGKSSIIHIMLNHSGNFFLFLLSPVLPSLWVAYVHFQIFQDEKRT
                     KKLLLPLCVINAINIITLIISQFYSWFYYIDSNNIRGPFFLVPASMTIILILAAAIII
                     VANYKKLDKRNCFSLILLAIPPFISILFQIRFYGISLMLNSLVLPLLTVLLTVQNHKI
                     YTDYLTGVSNREKLDAYLKEKVCTSTKEKSFSAILIDINNFKHINDTYGHNMGDDALE
                     TAAKLLKSCLRTNDFIARYGGDEFCVILDISDKKDLENLVSRINNCLEKHNRSGTHNY
                     KLEFSMGYAVYDYQTYKSPEEFLHYIDMLMYEDKRNYKNKIKVTV"
     gene            5720..6121
                     /locus_tag="PLG49_05275"
     CDS             5720..6121
                     /locus_tag="PLG49_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOQ16768.1"
                     /translation="MAIKWTPDLSVGVDHIDEQHQVWFEKANELFEAGKQQRAKEYIN
                     QMIDFLDEYTKYHFSDEEKYMAEIGYPELDAQKKAHASFINDLAKLKKDYNEGGGNLL
                     VIINANKMIINWLTNHIKTMDKKIGEYAKNL"
     gene            complement(6181..6852)
                     /locus_tag="PLG49_05280"
     CDS             complement(6181..6852)
                     /locus_tag="PLG49_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HOQ16769.1"
                     /translation="MIFSEKLQLLRKSKGFTQEELAERLDVSRQAVAKWESGQVYPDI
                     SNLIQISDLFNVSIDYLVRDQDCMLNISVDIKTDLDKLIDFRLEANRNTYAAAMNETN
                     STRLDSHDFRYTKGSFTYHDTYVGGEQFAGEEAIWQDGKAVYAMNYMGRVLGEQFSGN
                     FLKEVLKKADKNMPYRGPEYYRSGEYLYKSKVAGDFTWFQGYEEIYYNEIKVYECYFH
                     GGLIH"
     gene            complement(6964..7638)
                     /locus_tag="PLG49_05285"
     CDS             complement(6964..7638)
                     /locus_tag="PLG49_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ16770.1"
                     /translation="MKITVLHGQKHQGSTWHTTRLLLDYLITKGDECQEFYVNDIPPC
                     LGCFTCILKDEEECPHRSMTKPIIQALEQADIIIVETPNYCMGMTGQLKIFFDHMAYR
                     WMTHRPFAAMESKLAIAISTTAGAGARAATKQIAKQLFWWGIPKIYCINQTVAATKWE
                     DVKAQTKLKIEQKVRKVANRVKKDQVKIRRGPYQRFIFKMMGNMQKSGFGAAKDASYW
                     KEQGWI"
     gene            complement(7642..8250)
                     /locus_tag="PLG49_05290"
     CDS             complement(7642..8250)
                     /locus_tag="PLG49_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ16771.1"
                     /translation="MTTKDLILQTALRLFAERGYNGVSMRELAKEVGIKAASIYNYFS
                     SKEELFNHLLAEMQNRYEQIVKKLNIPMGKSKETALQYAALSEEGLQELAVSLFLYFA
                     KDEFAVSFRKMLIGEKYRNLAAGDLFKDMFIKEPLKYQTELFQKLIEQGKLLDADPKI
                     MALQFYAPLFLLLESYDETQEEEIIQSIKKHVSQFSRMYVRR"
     gene            8591..9418
                     /gene="speD"
                     /locus_tag="PLG49_05295"
     CDS             8591..9418
                     /gene="speD"
                     /locus_tag="PLG49_05295"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOQ16772.1"
                     /translation="MVLGVEKKLTLYGFNNLTKSLSFNIYDVCYAASDREKKDYIEYI
                     DEQYNSERLTNILCKVAEIIGANILNISKQDYEPQGASVNILIAEGQLLPHEINESCN
                     QGIVNGKALQKETVHAHLDKSHVTVHTFPEHHPDNAISTFRVDIDVATCGKISPLNAL
                     DFLINSFDSDIITIDYRVRGFTRDIHGKKYYIDHDIKSIQDYIDNNILKKYDAVDVNV
                     YQSNTFHTKMLIKEVELQNYLFNQDVYEIPPMKRLEIIDSLRKEMIEIFSGMNIYDT"
     gene            9422..10873
                     /locus_tag="PLG49_05300"
     CDS             9422..10873
                     /locus_tag="PLG49_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ16773.1"
                     /translation="MIRLDQNRAPLYEALEKHRKDRIVHFDVPGHKGGRGNKELSLFL
                     GEKCLKADVNSMKPLDNLNYPVSVIKEAQQLAAEAFGAHSCFFIVNGTTSAVQAMVMS
                     ACKAGEKILMPRNVHISALNALILSGAIPVYIDPGINNRLGIALGMSMADIKKAIEEH
                     SDAKAILVNNPTYYGICSNLKEIVELAHSHNMLVLVDEAHGTHFYFGDNMPVSAMKAG
                     ADMAAVSMHKTGGSLTQSSLLLCGKNVNHDYVRQIIGLTQTTSASYLLLVSLDLARKN
                     LSLNGKEIFNKISELAEYARKEINNLGGYYAFSHELVNNVNIYNFDTTKLSINTREIG
                     LAGIEVYDILRDDYDIQVEFGDLGNILAVVSAGDRAMEIERLISALYEIKRLYAKEPA
                     NMFDHNYIQPEVVYTPRKAFYANKKMMSLKDSKGKVSGEFVMAYPPGIPVLAPGEKIT
                     AEIIDYISYAKEKGSVMMGPQDMDLKNILVLEG"
     gene            10875..11720
                     /gene="speE"
                     /locus_tag="PLG49_05305"
     CDS             10875..11720
                     /gene="speE"
                     /locus_tag="PLG49_05305"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOQ16774.1"
                     /translation="MDLWFTEYYTKNARFSIKVDKHIVSVESEYQRIDVFDSYLFGRV
                     LVLDGYLMLTEKDEFIYHEMMTHIPMAVNPKIRDVLVIGAGDGGVVRELTKYDSIERI
                     DMAEIDKMVVDICKEYLPLTACKLDDERVKIYYEDGLKFVRSKENAYDLIIVDSTDPF
                     GPGEDLFTKEFYGNCYKALKDSGILVNQHESPYYPNDALAAKRTHQRINSVFPVAKVY
                     QAHIPTYPSGHWLFGFASKGLDPITDLDPDWNSLSIKTRYYNMDLHKGCFALPNYVKE
                     LFENA"
     gene            11713..12564
                     /gene="speB"
                     /locus_tag="PLG49_05310"
     CDS             11713..12564
                     /gene="speB"
                     /locus_tag="PLG49_05310"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOQ16775.1"
                     /translation="MLEKNIHNILGCDKDYQNSKIVIFGAPFDGTTSYRPGARFASAA
                     IRNESYSIETYSPYQDKDLEDIAVFDAGDLELPLGNPAKVLVQIENFAQNILNDEKIP
                     LMIGGEHLVSLGAVKAAVKKYKDLHIIHFDAHSDLRDEYLGENLSHATVMRRCWDLVG
                     DNRIFQFGIRSGSREEISWGKKHVHTCFFNFSQLDYVVDNLKDKPIYFTIDLDVLDSS
                     VFPGTGTPEAGGVTFCELLDAIKKVSKLNIVAMDITELAPPLDATGASTALACKLLRE
                     ILLYIYK"
     gene            12620..13819
                     /locus_tag="PLG49_05315"
     CDS             12620..13819
                     /locus_tag="PLG49_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOQ16776.1"
                     /translation="MGKALIIGAGGVGRVVAHKCAQNPDVFEEIIIASKTKEKCDQLK
                     KELDGGKAKIYSEQVDADNTEEVINLINKYKVDIVINVALPYQDLTIMDACLATKTDY
                     VDTANYEPPDVPKFEYKWQWAYKEKFEQAGITALLGSGFDPGVTGVFTAYAQKHYFDE
                     IHYIDILDANAGDHGYPFATNFNPEINIREITAPGSYWENGKWIETAPLEIKRTYNFP
                     EIGNMDIYLLHHEEIESLAKNIKNVKRIRFWMTFSQKYLTHLQVLQNVGMTSIEPIEF
                     EGKLIQPLQFLKAVLPDPATLGPRTKGKTNIGCVFQGIKDGKPRQYYVYNVCDHQECY
                     KEVGAQAVSYTAGVPAMIGAMMVLTGKWKKPGVFNVEEFDPDPFMDALNKNGLPWKES
                     FSPELLD"
     gene            13842..15005
                     /gene="nspC"
                     /locus_tag="PLG49_05320"
     CDS             13842..15005
                     /gene="nspC"
                     /locus_tag="PLG49_05320"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOQ16777.1"
                     /translation="MQIFKSIDLNIDLSQLPTPIYIVDERLIEKNLKILKEVIDRTGC
                     KILLAQKAFSMFYLYPLIGQYLTGTTASSLFEAKLGYEEMKGREVHIYAPAYREDEFD
                     EIIKICDHIIFNSFSQWNKYKDKIKASGKKIECGLRINPEYSEIETEIYNPCAAGSRL
                     GITLEQFKEQDLEGIDGLHFHTMCEQNSDVLKRTIEVVDKKFGDYIRKMKWINFGGGH
                     HITRTDYDIDTLIECIMYFKNKYNVEVYLEPGEAIALNAGYLAARVLDFVKNKMDIAI
                     LDTSAACHMPDVIEMPYRPKIIGAGEPGEYPYNYRFGGLTCLAGDIIGDYSFKEPLKE
                     GDILVFTDMAIYTMVKNNTFNGVNLPAIASYNDKEGLKIIRQFGYEDFKGRLS"
     gene            complement(15083..15844)
                     /locus_tag="PLG49_05325"
     CDS             complement(15083..15844)
                     /locus_tag="PLG49_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102920.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOQ16778.1"
                     /translation="MIFYFSATGNSKYLASRIAKETKNRLISITDCIKNNEYAFKLKE
                     GENLGIVSPIYYWGLPSIVDDFLEYFQLVVSHRPYTFFAATYGTVTGQADTFVKRHLK
                     RLGLELSASYCVRMPDTWTPIFDLNDPVKVSKVNQKAEIQIDKIIKQIKNTESGNFLS
                     FRMPMPAVKLCRYIYEKVRETKHLSVEDSCIGCGLCAQNCPVDAIEIQSGRPVWVKDK
                     CSICLACLHRCPQFSIQYGKFTKKHGQYLHPCMKE"
     gene            complement(15859..17070)
                     /locus_tag="PLG49_05330"
     CDS             complement(15859..17070)
                     /locus_tag="PLG49_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ16779.1"
                     /translation="MSNSRQILIVGSGNAGFSAAKAARTQDPEAKIIMFGEENQLPYY
                     RLRLCDYIGKNLAYDKLEINHEEWFEKNKIQLELSSKVTAIDIKAKKISVNGKEYGYD
                     SLVLATGSTPIMPSFEGKELSGVYTIWTTDDIVKINKELLAARKAVVIGGGLLGLEAA
                     HKISEMGTEVSLIECMPRLLPKQLDEEGSEIFKDKVQSLGISVLCGKSVEGFVGDHNG
                     HVMKVGMTDGSFFEADLVIVAVGVAPNTSICQSRGINMDRCLIVNEKMETNIKDIYAA
                     GDVASVNGRWYGQWAVAGKQGQVAGINAAGGNAVYKSEDLPYILATMNTRVVSVGDTG
                     LNLTDNKKAAYEIDQMIDKERFSYSRLVFKNGVFVGYMLVGEPAKVFNKLQPLMNTTV
                     TAQTIKNILWQ"
     gene            complement(17175..18788)
                     /gene="ptsP"
                     /locus_tag="PLG49_05335"
     CDS             complement(17175..18788)
                     /gene="ptsP"
                     /locus_tag="PLG49_05335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOQ16780.1"
                     /translation="MKKGIAASKGYAIGKVMVKSQQEIKITDESISDIETEKEKFLLA
                     VEESKAQLEKLKEQAAQSMGEEQAEIFETHIMMLEDREFIDAIISEIENNHVNALKAI
                     SQVSSFFIAQFEAIEDEYLRERAADLKDITNRLMANLTGNQTDFKIEEENIVVVAHDL
                     TPSDTAQLDKDKVIAFLTDVGSRTSHSAIMARTLEIPAVVGMGDISSSVKNGDMVIVD
                     GFTGTVIINPGNDELEIYKKKIKEYQEELKELKKLVSCETRTKAGKRIYVAGNIGKPE
                     DIYQVIENGGDGIGLFRTEFLYMDRDTAPSEEEQFESYRFVLEKMGDKKVVIRTLDIG
                     GDKTLPYMDLPKEMNPFLGYRAIRLCLDQKELFKTQLRALLRASVYGNLSVMFPMISG
                     LQEFLAAMKIVEECKKELDVSGIPYSKDIEWGIMIEIPAAAICADELAQYIDFFSIGT
                     NDLIQYTLAVDRMSEKVSYIYNPMHPAVLRLIKMTIDGAHKYGKWVGMCGEMAGDETA
                     IPILVEYGLDEFSMSASSILPAKKIIMGL"
     gene            complement(18825..19088)
                     /locus_tag="PLG49_05340"
     CDS             complement(18825..19088)
                     /locus_tag="PLG49_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ16781.1"
                     /translation="MVKQDIVVVNKSGLHARPASNLVKAASKFKSDITILFQEKVINV
                     KSLLNVLSAGIVCGTEITLVCDGEDEQEALKTLTEMIESGLGE"
     gene            complement(19131..19847)
                     /locus_tag="PLG49_05345"
     CDS             complement(19131..19847)
                     /locus_tag="PLG49_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ16782.1"
                     /translation="MDYKVPKYVQIKTDIINAIKSGKLKPGDKVDSESVLKKKYNVSS
                     ITVRKAFNDLINENYLVGVQGVGTFVAKEPINRRLTSISFSDELLQQGYKIDMQVDKI
                     EEVTDERIAEKLGLAPDQTLICVKRVRLVNNEPVAYQCSFMDSQMLSLEQAQKIHEHK
                     SFYKTLAEYNIIPVGATENYSIKTVNDSHIAKLMKIKKGTDTFFVKRTTFDETGKVIE
                     YAETYFNKNWYSVTVEVKFG"
     gene            complement(19931..20326)
                     /locus_tag="PLG49_05350"
     CDS             complement(19931..20326)
                     /locus_tag="PLG49_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ16783.1"
                     /translation="MKRVVVMGHGGYAEGVRKNLEMIVGVPEFMHFLDLTKEDDLDRF
                     KARVEDLVKQFDDDDEVLFVCDLLGASPFRVAAELCISKAGKYYTLAGLNAMAIMELS
                     MIEDSELSIEELANHAVETTKTAVAKFPE"
     gene            complement(20539..21402)
                     /locus_tag="PLG49_05355"
     CDS             complement(20539..21402)
                     /locus_tag="PLG49_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOQ16784.1"
                     /translation="MKKISEKTLKRSFLNWFFWNGCSQQAESMLGIAFGQSMAPVIDE
                     LYDKKEEKAEALKRHITLFNTESQVGSICNGIVCGLEEANANGNCTPELISSVKVALI
                     GPTSAIGDSLWVATIIPILLTICLSISQASLATGWLGPVLYMLVYPIGTFILSWYLFK
                     LGYRSGLEGMQTFMSSGRLNSLTDTMTVLGLIVVGALTASFVSLPLPIQIVRDVFDAT
                     TGQILENQVIFDADKVVNSIFPNILPLLLTLAVYYLYSKKKWSPLKLMGLIFVLACLL
                     TLLGYATGVYA"
     gene            complement(21402..22196)
                     /locus_tag="PLG49_05360"
     CDS             complement(21402..22196)
                     /locus_tag="PLG49_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOQ16785.1"
                     /translation="MQIYQIILIALFIYLGSIGSIVGNTIGWYTLGRPLVASFVVGLI
                     MGDVQTAILVGISLQIMYMGNVTPGGAVAWDLSYATYIGTAAALVFGKGLDTAQVIGL
                     AVVFAGIGGLVGQIMWNLSYALNLPLNRVAQRYAEAGETKKMFIPNVVLGQLIGFACR
                     FIPAVIVLTSMTAASAQGDFASLIPGWLTTVLGVFGGMMAALGMGIILSFLFKKKYHV
                     VIFLAGFILVTYFNLSTMAVAVLAIIIAVLYYVAVTNLEAKRGEVQ"
     gene            complement(22212..22703)
                     /locus_tag="PLG49_05365"
     CDS             complement(22212..22703)
                     /locus_tag="PLG49_05365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOQ16786.1"
                     /translation="MGKTYIRVDDRLIHGQTIVAWCPALSIKEIIAIDDESAKNPMLK
                     SILTMGVPQNYKTHIVTTEEAKKLLKEESPINRLVIVRFPEKLEELKEEMEQVEMLIL
                     GNIAKRPDTVHKVSGATGIFYLSDRDVSIIDEIASKGIEVCFQQVPTTSKTTWEAFKK
                     SIN"
     gene            complement(22747..23646)
                     /gene="murQ"
                     /locus_tag="PLG49_05370"
     CDS             complement(22747..23646)
                     /gene="murQ"
                     /locus_tag="PLG49_05370"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOQ16787.1"
                     /translation="MSDIRIDHLKTESRNPRTLNLDTMSTMELLRIMNEEDQKVIDAV
                     KEALPQIEQCVQLVIKALNNNGRLIYIGAGTSGRLGILDAVECAPTFSTTDEVIGIIA
                     GGEKAFIKAVEGAEDSEELAEKDLDAVSVGEKDVVVGIAASGRTPYVIGALKHAKKHG
                     AKAVGIACNKNSELAKYADVTIEVDAGPEILTGSTRLKAGTCQKLILNMISTASMVGI
                     GKAYGNLMVDMKPTNLKLKERAKRIVMESTGCDKKTAEKILEETDYSMKEAIVMILTG
                     VSREEAERRIIENKGFVRKCIEQ"
BASE COUNT         7590 a         4573 c         3783 g         7786 t
ORIGIN      
        1 tactaactct atctgtcttg aaatattatt tttcatttct agttattttt tcttttttct
       61 tatctaaact aattcctttt ttcttcaatt atgttaccat attattaaaa tagtcataat
      121 tatttaattt ttcttaccta tacacttatc tatgtttccc caatactata actttctaac
      181 gagatctact gaaatttgta actattaata gtgcaattat ttgtttttta aattttttca
      241 taaagttctt cctaaaattt atatacatta aatcatgaca taattcaaag ctaattgacc
      301 tattttgtat gacttttcta aagaatagca tttctgaaat attctccaag aataacgtgt
      361 aaggggtgaa aatatgagat tagaagaagc gaataaaaga ttaaataacc ttgttggtat
      421 accttttgaa attcttttct cgcaaaatga tttgaacatg attatcaaaa ataaaggcaa
      481 gacgggacag cttttagaac ttgcattggg catgaatttg tccagctcaa atctagactt
      541 tgatgatgga gaattaaaaa ctaataaatg cgatgctgct ggaaatccta aagaaacaat
      601 atttattact caaattagta gtataattga tgaattgatc gataaaaaac cattcgagca
      661 aactcactta tataagaaga taaaaaacat cttatatgta cccgtatgta aagaagggaa
      721 tccaagcgat tggaggtttt tacctagtat tcatattgat ttaagttcac cgcgctattc
      781 agaattactt aatatttgga aacaggatta ttattctata tgcgaacaga taaggaatca
      841 tattgaaaaa agtccggatg gatttataca tacttcaagt ggaaagcata ttcaaattag
      901 aagtaaagat tcaaaaccat atcatccaat ctattcagat atttataagc gcaatatttc
      961 ggataagaat tacgccttct acttcaaaaa acaatttgtt attgatataa gaagcatgaa
     1021 tacataattg tttttggtat aatgaaaaca gcatcaggac tatttgtgtt accttaatta
     1081 tctttcatgc cttctatgtc acctttttca tgttatttta ctttttaaca tttccatttg
     1141 catgttagat ctcttttaag ccttccaaag gtctttcatc tctcgtttcc ttaatactta
     1201 gtcaacagat ttcaaaaata accataaaaa tagcacatac caacctggta agtgctattt
     1261 attctttatt aattaatttt tctaattgct ctagtatatc ttcattttta caaatcagga
     1321 aagggccata atctatatca taacataatt gaaaatctac atctttatat tttccttccc
     1381 aaatatctaa ttcaacaatt tctatatagt ttataaactt tgtattcata tcattttcta
     1441 agattgactt agcttttaag aaaatttcac gttcatgaat cctatccaaa tttttaaaat
     1501 aaaacctatc ctttttttca tctctgaaag ttaacatttt tcttccctac tcctaacatt
     1561 atattctctt tatttattaa gcgcgcctaa aaacagctaa agctcatctt aacattaaaa
     1621 tagccctttc aaccaataaa aaaataggat ttttagaaat cctaccccaa ctattaaaat
     1681 aaaaccaagt ttcatcatta attcctttaa gcttgataac tttcagcgtt aaaaaactcc
     1741 aaaatagtcc tcttataata actgccagaa agtatgtaat aattatatca tctaactacc
     1801 tcaaatacat attaaaaaac attttaacga tttatatatt atttgtcaac aaattttaat
     1861 aattcacaat ttttgtgagc taaaatgtac tatcttgacc ttatcagttt acaatatctt
     1921 tttatataat tattccgtta aaacaaaatt gatgctttct ttacttgtga aatataataa
     1981 agaattttat gaaaaaaatt acccctcaat ccttttcatc tttctaaatc aaaattgcag
     2041 ataacttaat gtatctatct gcaatttatt ttttaaaaat atcttattaa gcttttcaat
     2101 attaaatcag caaaaactta tgatattaat gaatcagata atacaaaatc agcaatatcc
     2161 ccagaaaaat caggttaagc aaagtaaaga ccaaaagata aacggcattg tgtttccctt
     2221 ctacggttcc ataatattta aaagaaatca aactcgccat ggatgcaatg attgtcccaa
     2281 gtccacctaa attagtccct ataattaaag tggatatatt gtcggtaaaa gctgataaaa
     2341 gtatcgccgc aggcacgtta ctgataacct ggcttgcaaa cacagatgtc agtaattcat
     2401 gtccatggat aatgctttgc agccaatgat tgatttctgg aatcctcttt atatttccaa
     2461 taaagataaa taaaaataca aaggtaaata gtaacgaata atctgcttta ccaagcactt
     2521 tacgatctaa cagaaaaaca actaccagaa taactagccc tgctatatag gcagaaataa
     2581 tacgggttac actggttaaa gcaaataaaa aaagcaagaa ataaacaaat gctttctttt
     2641 tttctgcttc tgtaaggtta agctttgatt gtactttgac agaaacgctt tttttatcaa
     2701 ttaagatgac acaagcaact aataaagcta taagtgaaag caaggtataa ggcagcatta
     2761 atgagataaa gctactcagt ttcataccac ttaaagaaaa aagatataaa ttttgaggat
     2821 tacctatggg cgtaagcata ctgcctaagt tgctggcaat ggtttgcagc acaatcgttt
     2881 gaattaaata attttctaaa ccagccatta aaagcacatc aattgtaaag ggtacaaaag
     2941 cgataagcgc aacatcattg gtaataagca tgctaaatac aaaacataat gtcaccaaaa
     3001 ccagagatag agtccgtaca ttagaagctt tttgaagcat ccagccccct gtcattgaaa
     3061 atatacccaa gcttcgaagt ccagccataa taagcattaa actaaataag ataattaagg
     3121 tatgaaagtc tatgtagctg atatattgtt tatccaatgg tacaaaggtc atagatacaa
     3181 gggctagtag caaagatatg caaagtacga gttctttttt tataaaattt aaaattgatt
     3241 tcatccagtc attactcctt attgtcgttt atctgtttat taacattcct aaaaatctat
     3301 cccgctattc cggattaatc aaacaggcct ttagtatttt ccatgaagca ctttaaacaa
     3361 aaaaatcgac cgacgtttac gtcggtcttt attcattaaa actgcgtaaa tacatcacag
     3421 ttgattattt tattgtattt ttaaattaat aaaacttctt gcctcaaaaa atttcaatag
     3481 acattatatg agaaaatacg aatcatttca atatttttcg aagttatttt taatatatta
     3541 aaagggcttt tgagctaagt ctccattaaa gaggaaactg ccaaaaaccc tgaactgaaa
     3601 tcttaaatat ttttatctta ttattttata aatttttcat tgagtgtttt taaaaactta
     3661 aacaaaaaat agatttggag cttttttaaa atttaattac ttttgactct tcagtaaatg
     3721 aataaaaagt tgctgttgca tcttcaaaat agagaacttc tgtattatca tcttctggtg
     3781 aatacaagct ttgaagttcc ggataattat ccagcaaatg ctttttagct tctactctgt
     3841 catctcttac aagttttcca gctagtctaa gccacttgcc atcaccccat gcacagattt
     3901 ctaccttggg attcttttga atctgcctgg atacatcttt tacttttcca gtctggatat
     3961 aaagtttacc ttcaaaaata tctacagtac caaagggtct gactcttggc tgatcacctt
     4021 ccacagttgc taaataataa gttccacatt tttttaagaa ttcatatact tcttccatac
     4081 ttttttcctc ctgtctattt actaaaatta aatatttggc tctaaattaa atctacttca
     4141 aaaagcccaa aaaaatcaag gcttacaagt aagcctttac cctttattta tctttattat
     4201 actgtaactt taatcttatt tttataatta cgtttgtcct catacatcaa catatcaata
     4261 taatgtaaaa actcttccgg actcttgtaa gtttgataat cataaactgc atagcccata
     4321 ctaaactcaa gcttgtagtt atgagtacct gacctgttgt gcttttcaag acagttgtta
     4381 attcttgaaa ccagattctc taaatctttt ttatcagaga tatctaagat aacgcaaaat
     4441 tcatcgccac catatctggc aataaaatca tttgtcctga gacaactttt aagcaatttt
     4501 gctgctgtct ccagggcatc atctcccata ttatgaccat aggtatcatt aatatgttta
     4561 aaattattaa tatctattaa tatagcagaa aaactctttt ctttagtcga agtacatacc
     4621 ttttccttta aataagcatc aagtttttct cgattactga ctcctgttaa ataatcggta
     4681 taaatcttat gattttgtac agttaacaat actgttaaaa gcggtaaaac gaggctgttt
     4741 aacatcagtg atatcccata aaagcggatt tgaaataaaa tactgataaa tggcggtata
     4801 gccaggagta tcagcgaaaa gcaatttctt ttatccaact ttttgtagtt tgcaacaatg
     4861 ataatagctg ctgcaagtat caaaataatt gtcatcgaag ccggaactaa aaagaaaggt
     4921 cctctgatat tattggaatc aatatagtaa aaccagctgt aaaactgcga tattatcaaa
     4981 gtaattatat taattgcatt aatgacacaa agaggcagta ataacttctt tgttcttttt
     5041 tcatcctgaa atatctgaaa atgaacataa gctacccaca aagaaggtaa aaccggactt
     5101 aataaaaaga gaaaaaaatt tcctgaatga tttagcataa tatgaatgat agaagattta
     5161 ccgtcaaatc tgcttaaaat atctacagtg agcaaaatta ccgtaacatt taatatctgc
     5221 atatatagtt tgtctgccat agaatctctt ttaaaaaact tagaggcaaa aaaatagata
     5281 atgaaaatga tcacgataga atatatatta aaaataatgt tttctgtctg aaccatgatg
     5341 tcaactccta ttgttaaaaa attgccaaaa agaataacgc tttgaaacaa tttgttctgc
     5401 atattctgca atgataaaga aaaaataata tagtcgtata caattatata atagtccctt
     5461 agacctatct atgtcaacat aattcaattt ttaaatcttt ttttaatcta attgtaatat
     5521 tgtcaaatca gtaacagatt gatgtttgtt gtttttaaat acgggttagt gaattggtgg
     5581 aaacataaga aactgaaaat atatctaaaa gtttccttgt gcttcatcga catattgtgt
     5641 cattcttttt caaatctatt gaatttattt taaaaaaact atacaattaa attaaaataa
     5701 ttataaggag agggtatata tggcaataaa atggactcct gacttatcgg ttggtgttga
     5761 ccatattgat gagcaacacc aggtatggtt tgaaaaagct aatgaattat ttgaagccgg
     5821 aaagcaacaa agagctaaag aatacatcaa tcaaatgatc gacttcttag atgagtatac
     5881 aaagtatcac tttagtgatg aagaaaagta tatggcagaa atcggatacc ctgaactgga
     5941 tgctcaaaag aaagcccatg caagttttat taatgacctt gcaaaactaa aaaaagatta
     6001 caatgaaggc ggcggtaatc tccttgttat cattaatgct aataaaatga ttatcaactg
     6061 gcttaccaat cacatcaaaa ccatggataa aaaaattggg gagtatgcaa aaaatcttta
     6121 attcaaacac ctggaagtaa agaagcaagg agacggttcc ttgcttcact cttttttatc
     6181 ttaatgaatt aacccaccat gaaaatagca ctcatatact tttatctcat tataatagat
     6241 ttcttcataa ccctgaaacc aggtaaaatc ccctgcgact ttacttttat ataagtattc
     6301 tccggaccga tagtattctg gtcctctgta aggcatattt ttatcggctt tttttaaaac
     6361 ttcctttaag aaatttccgc taaattgctc tcctagaacc cgtcccatat aattcatcgc
     6421 ataaacggct tttccatcct gccaaatggc ttcctctcct gcaaattgtt ctccgcccac
     6481 atatgtgtca tgatatgtaa aagacccttt ggtataccta aagtcatgag aatccagtct
     6541 ggttgaattg gtctcattca ttgctgctgc ataggtattt ctatttgctt ccagtctaaa
     6601 atcaatcagt ttatctaagt ctgtttttat atccacagaa atattaagca tacaatcctg
     6661 atccctaacc aaataatcaa tggatacatt aaataaatca ctaatctgaa tcagatttga
     6721 aatatccggg taaacctgtc ctgactccca tttagccaca gcctgtctgg atacatcaag
     6781 tctctcagcc aattcttcct gcgtaaatcc tttacttttt ctcagtaatt gcagtttttc
     6841 tgaaaaaatc attttcatca cctcaaattt attatacaaa agccctgccg ccataaacat
     6901 aagtctatat ttgcaatttt gctactttta ttatacaagc aaatctttct tctgcttttt
     6961 cttttatatc cagccttgtt ctttccagta agaagcgtcc ttagctgcgc caaagcccga
     7021 cttttgcata ttccccatca ttttgaatat aaacctttga taaggtccac ggcgaatttt
     7081 tacttgatct ttcttaactc tattggcaac tttccttacc ttttgctcta tttttaactt
     7141 tgtttgagct ttcacatcct cccattttgt cgccgctacg gtctgattaa tgcaatatat
     7201 tttgggaatt ccccaccaaa agagttgctt tgcaatttgt tttgtggctg cccttgcacc
     7261 cgcaccagct gttgtggata tggcaatggc taatttgctt tccattgcag caaatggacg
     7321 atgggtcatc cagcgataag ccatatgatc gaaaaaaatt tttaattgac ccgtcatacc
     7381 catacagtaa ttgggtgtct caacaatgat gatatctgcc tgctcaaggg cttgaataat
     7441 tggctttgtc atacttctgt ggggacattc ttcctcgtct tttaatatac aagtaaaaca
     7501 gccaagacaa ggggggatat catttacata aaactcttga cattcatccc ccttagttat
     7561 gagataatct agcaagagac gtgttgtgtg ccatgtactt ccctggtgct tttgcccatg
     7621 taaaaccgta atcttcattt tttacctcct gacatacatt cttgaaaact gactgacatg
     7681 cttttttatg ctttgaatga tttcttcttc ctgagtttca tcgtaacttt caaggagcaa
     7741 aaataaaggc gcataaaatt gtaatgccat aatcttagga tcggcatcca gtaattttcc
     7801 ttgttctata agtttttgaa aaagttctgt ctgatatttt aatggttctt tgataaacat
     7861 atctttaaaa agatccccgg cagctaaatt acgatacttt tctcctataa gcattttgcg
     7921 gaaggatact gcaaattcat cttttgcaaa atataaaaat aggcttacgg caagttcttg
     7981 caaaccttct tctgataggg cagcatactg caatgctgtt tctttagatt ttcccatagg
     8041 gatattgagt ttttttacta tttgctcata acgattttgc atctctgcaa gtaaatgatt
     8101 aaaaagctct tccttacttg aaaagtaatt atagatagag gctgctttga taccaacctc
     8161 ctttgccagt tctcgcatcg aaacgccatt atagccccgt tcagcaaaaa gccgtaatgc
     8221 ggtctgaaga attaagtctt tggttgtcat aaaacgcacc tcacaaaaac aaattgaaaa
     8281 tataaaatga aaaactaaca aatgttagtt ttattatacc taacgcttgt tagtttatca
     8341 atatgaataa aataatttct attttgcact ttttgttgtc atgtcctgat gattgccata
     8401 aaaagtcttc aattaaaacg ccattggctt ttagtctatt cattttattg gaaaccccag
     8461 ggcttttaaa aaatcctcat tttatttcaa aaaaaaaata tatattgcat ttgcctataa
     8521 aatatgtaat aatcttcttg cttaatgcac atcctattct ttagtatttt tagtatccca
     8581 tagaaaggta gtggtgttag gggtggaaaa aaagttaacc ttatatggtt ttaacaatct
     8641 tacaaagtcc ctgagtttta atatttatga cgtatgctat gcagcaagtg atcgagaaaa
     8701 aaaagattat atcgaatata tagatgagca gtataattct gaaagactca cgaatatttt
     8761 gtgtaaagtt gctgaaatta tcggtgctaa tatactgaat atttctaagc aggattatga
     8821 acctcagggg gcttctgtta atattttgat cgcagaggga caattgcttc cccatgaaat
     8881 aaatgaatct tgtaatcagg gcattgtaaa tggaaaggct ttacaaaaag aaaccgtaca
     8941 tgcccattta gataaaagtc atgtaaccgt acatactttc ccggagcacc atcctgataa
     9001 tgctatttct actttcagag tagatattga tgttgcaacc tgtggtaaga tttctccact
     9061 taatgcactg gattttttaa taaacagttt tgattcagac ataataacga tagactaccg
     9121 agttagaggc ttcacccggg atatccatgg taaaaaatat tatattgacc acgacatcaa
     9181 gtccatacag gattatatcg ataataatat tttaaaaaaa tacgatgcgg ttgatgtaaa
     9241 tgtctatcaa tcgaatactt tccataccaa aatgcttata aaagaagtgg aactgcaaaa
     9301 ttatcttttt aatcaggacg tttatgaaat acctcctatg aaaaggcttg aaattattga
     9361 cagccttcgc aaagaaatga ttgaaatttt tagcggtatg aatatttatg atacatagga
     9421 ggtgattagg ttggatcaaa acagagctcc tctttatgaa gctttagaaa aacacagaaa
     9481 agatagaatc gtacactttg atgtcccagg acacaaaggc ggccggggca ataaagagct
     9541 gagtctcttt cttggagaaa aatgccttaa agcagacgtt aattccatga aacctctaga
     9601 caacttaaat tatcccgtat cggttattaa agaagcacag caattggctg cggaagcttt
     9661 cggagcccat agctgctttt ttattgtaaa cggtacaaca tcagctgttc aagccatggt
     9721 gatgtctgcc tgcaaagcgg gtgaaaaaat actcatgccc cgtaatgtcc atattagtgc
     9781 tttaaatgcc cttattttat ctggagccat tcctgtttat atcgatcctg gtatcaataa
     9841 ccggcttggt atagccctcg gtatgtccat ggctgatatt aaaaaagcca ttgaagagca
     9901 ttctgatgcc aaggcaattt tagtcaacaa tccaacctat tatggaattt gctccaattt
     9961 aaaggaaatt gttgagctgg cgcatagtca taacatgctg gtactggtcg atgaagctca
    10021 cggtacccat ttttattttg gcgataatat gcctgtttcc gccatgaaag cgggtgcaga
    10081 catggcagca gtcagtatgc acaaaactgg tggttccctg acacaaagct ccctgctgct
    10141 gtgcggaaaa aatgtaaacc atgactatgt acggcaaatc atcggcctta cccagacaac
    10201 cagtgcttct tatttactcc ttgtgtccct ggatttggct agaaaaaatt taagcttaaa
    10261 tggaaaggaa atatttaata aaattagcga actggctgaa tacgccagaa aggaaattaa
    10321 taatctcggg ggctattatg ccttttccca tgaactggtg aacaacgtta atatttataa
    10381 ctttgataca accaaacttt caatcaatac aagggaaatt gggctggcag gaattgaagt
    10441 ttacgatatc ttacgggacg attatgacat tcaggttgaa tttggggatc tgggcaatat
    10501 tttagctgtt gtatctgctg gtgacagagc aatggaaatc gaacggctta tttctgccct
    10561 ttatgagatt aaaagacttt atgccaaaga accagccaat atgtttgacc ataactatat
    10621 tcagcctgaa gtggtctata cgccacgaaa agccttttat gccaataaga aaatgatgtc
    10681 tttaaaagac tccaaaggta aagtcagcgg agaatttgtt atggcttatc cgcctggcat
    10741 accagtttta gcccccggcg aaaaaatcac ggctgaaatt attgattata tcagttatgc
    10801 caaggaaaaa ggcagtgtaa tgatgggccc tcaagatatg gatcttaaaa atatcttggt
    10861 attggaggga tgatatggat ttatggttta ctgaatatta tacaaaaaat gcccgctttt
    10921 ctattaaagt cgataaacat atcgtcagcg tagaaagtga atatcagcgt atagatgtct
    10981 ttgattctta cctctttggc agggttttgg tgctggacgg ctacttgatg ttaacagaaa
    11041 aagacgaatt tatctatcat gaaatgatga cccatattcc aatggcagta aacccaaaaa
    11101 tccgtgacgt tcttgtgatt ggagccggcg atggcggtgt tgtaagagag cttactaaat
    11161 acgactccat tgagcggatt gatatggcag aaatcgataa aatggtcgtg gatatctgta
    11221 aagaatattt gcctctgacc gcatgcaaac tggatgatga acgggtaaaa atctattatg
    11281 aggatggtct taaatttgtc aggagcaaag aaaatgcata cgatttaatc atcgttgatt
    11341 caacggatcc ctttggacca ggagaagacc tttttactaa ggaattttac ggcaattgtt
    11401 ataaggcatt aaaggactcc ggcatcctgg ttaatcagca tgaaagtcct tattatccca
    11461 atgatgcctt agcagccaaa agaacacatc aaaggataaa ttccgtattc cctgtagcca
    11521 aagtctatca ggcccatata ccaacttatc cgtctggtca ttggcttttt ggctttgcct
    11581 caaaagggct tgacccaatt acggatttag atcctgactg gaattctctc agcatcaaga
    11641 ccaggtacta caatatggat ttgcataaag gttgttttgc attaccaaac tatgtaaagg
    11701 agctttttga aaatgcttga gaaaaatatt cataatatct taggctgtga taaagattat
    11761 cagaacagta aaattgtcat ttttggagcg ccttttgatg ggacaacctc ttaccggcca
    11821 ggggctagat ttgcaagtgc tgccatccgc aatgaaagct actccataga aacctatagc
    11881 ccttaccaag ataaggattt agaagatatc gctgtctttg atgcagggga tttggaactg
    11941 cctttgggca atccggcaaa agtccttgtg caaattgaaa attttgcaca aaatatctta
    12001 aacgacgaaa aaattccgct tatgataggc ggcgaacatc tggtaagttt aggagcagta
    12061 aaggctgcgg taaaaaaata taaagacctt catattattc attttgatgc ccattcagat
    12121 ttaagagacg aatatttggg tgaaaacctg tcccatgcta cagtgatgag gcgctgttgg
    12181 gatttagttg gagacaacag gatttttcaa tttggcataa gaagtggctc cagagaagaa
    12241 attagctggg gtaaaaaaca tgttcatact tgttttttta actttagcca gttggattat
    12301 gttgtagaca acttaaaaga taaaccaata tattttacca ttgatttaga tgttctggac
    12361 agctcagtat ttcctggaac aggaacccct gaagcaggtg gtgttacttt ttgtgagctt
    12421 ttagatgcga ttaaaaaggt atctaagtta aatatcgttg ccatggatat taccgaactt
    12481 gccccgcctc ttgatgccac aggtgcatca acggcgctgg cttgcaagct tttacgtgaa
    12541 atcctcttat atatttataa ataaattttt agaggaattt taattaaaga aatcttattt
    12601 ttgaaatgga ggttttgata tgggaaaagc attaatcatt ggtgccggtg gtgtcggcag
    12661 agttgtagcg cacaaatgtg cacaaaaccc tgatgtgttt gaagaaatta ttattgccag
    12721 caaaacaaaa gaaaaatgcg accagctaaa aaaagagtta gatggtggca aagccaaaat
    12781 ttattctgaa caagtagatg ccgacaacac tgaagaagtc atcaatttaa ttaacaaata
    12841 taaagtggac attgtcatta atgttgcttt gccataccag gacttaacca taatggatgc
    12901 ttgccttgca acaaagacag actacgtgga tactgccaat tacgagcccc cggatgtgcc
    12961 aaaattcgaa tacaaatggc agtgggcata taaggaaaag ttcgagcagg cgggtattac
    13021 agccctttta ggcagtggtt ttgaccctgg tgttacgggt gtgtttactg cttatgctca
    13081 aaaacattat tttgatgaaa tccactatat cgatatcctc gatgctaatg caggagacca
    13141 tggctatcct tttgccacaa atttcaatcc tgaaatcaat attcgtgaaa ttacagctcc
    13201 aggcagctac tgggaaaatg gaaaatggat tgaaaccgcg cccctggaaa ttaaaagaac
    13261 ttacaatttc cctgaaatag gtaatatgga catttatctg ttacaccatg aagaaattga
    13321 atccttagct aaaaacatta aaaacgtaaa aagaattaga ttctggatga cattctctca
    13381 aaaatattta acccaccttc aagttctgca aaatgttggc atgacctcca tcgagccaat
    13441 tgaatttgaa ggtaagctaa tacaaccttt acaattctta aaagcggtat tacctgaccc
    13501 tgcgacttta ggcccaagaa ccaaaggtaa aaccaatatc ggctgtgttt tccagggaat
    13561 caaagacggt aaacccagac aatattatgt ttataatgtt tgcgatcatc aggaatgcta
    13621 taaggaagtt ggtgctcagg ctgtttccta tacagcaggt gtgccagcca tgattggtgc
    13681 aatgatggta ctgacgggca aatggaaaaa acctggtgta ttcaatgtag aagaatttga
    13741 tcccgatcca tttatggatg cattaaataa aaacggactg ccctggaaag aaagtttttc
    13801 accagaatta ttagattaaa ggtgagccta tggagaaaaa tatgcaaatt ttcaaaagta
    13861 ttgatttaaa tattgattta tcccagcttc caacgccaat ctatatcgtg gatgaaaggt
    13921 taatcgaaaa aaaccttaaa atattaaaag aggtgattga taggaccggc tgcaagattc
    13981 ttcttgccca aaaagccttt tccatgttct atctctatcc tcttattggt cagtatctga
    14041 ctggtacaac agccagttct ctttttgaag ccaaattggg ctatgaagag atgaaaggaa
    14101 gagaagttca catctatgcc cctgcctaca gggaagatga gtttgatgaa attattaaaa
    14161 tatgcgatca cattattttt aattcatttt ctcagtggaa taaatacaag gataaaataa
    14221 aagcctctgg caaaaagatt gaatgtggtc ttcgaatcaa tccggaatat tcggaaattg
    14281 agaccgaaat atataacccc tgtgctgcag gctccagact gggtattacc ctggaacagt
    14341 ttaaagagca ggatttagaa ggcatagatg ggctgcattt tcacaccatg tgtgagcaaa
    14401 actctgatgt cctgaaacga actatagaag tagtagacaa gaagtttggc gattatatca
    14461 ggaaaatgaa atggattaac tttggcggcg ggcatcatat tacaagaaca gattatgaca
    14521 tcgatacact tatcgaatgt atcatgtatt tcaaaaataa atataatgta gaagtttact
    14581 tagaacccgg tgaagccatt gctttaaatg caggctatct ggcagccaga gtccttgatt
    14641 ttgtcaaaaa taaaatggat attgccattt tagatacttc tgcagcatgc cacatgcctg
    14701 atgtcatcga aatgccttac agaccaaaaa tcatcggtgc tggtgaacca ggtgaatacc
    14761 catataatta ccgctttggt ggtctgacct gtctggcagg agacattatc ggagactatt
    14821 cctttaaaga acctttaaaa gaaggcgata ttctagtctt tactgatatg gccatctaca
    14881 ccatggtaaa gaacaatacc tttaatggag taaatctccc cgccattgca agttataacg
    14941 ataaagaggg cctgaaaatc atcagacaat ttggatatga agattttaaa ggacgtctgt
    15001 cataaataat aacgagacaa ggggacggtt gcgctgtttc aagaaacttg aaacagcaca
    15061 accgtcccct tgtttcatct tttcattctt tcatgcaagg atgcagatac tgcccatgct
    15121 ttttggtgaa ttttccatat tgaattgaaa actgaggaca acggtgtaaa caagcaagac
    15181 agatagaaca tttatctttc acccagacag gtcgtccgga ttggatctcg atggcatcca
    15241 caggacaatt ttgagcacac aagccacagc ctatacagct atcctcaacg cttaaatgtt
    15301 tggtttcccg cactttttca taaatgtacc ggcataactt taccgcaggc attggcatac
    15361 gaaagcttaa aaaattccca gattcagtat ttttgatttg ttttatgatt ttatcaattt
    15421 gtatttcagc tttttgattg actttgctaa cttttacagg atcgtttaaa tcaaatatgg
    15481 gagtccaggt atctggcatt cttacgcaat aactcgctga aagttcaagg cctaatcttt
    15541 ttaaatgcct tttaacaaaa gtatctgctt gtccagttac tgttccataa gttgccgcaa
    15601 aaaatgtata tggacgatgg ctaacaacta attgaaaata ctctaaaaaa tcatctacga
    15661 ttgaaggcaa accccaataa tagattggag atactattcc taagttttcc ccttccttta
    15721 gcttaaaggc atattcatta tttttgatac aatctgtaat ggaaatcagt ctgttcttcg
    15781 tttcttttgc aattcttgat gccagatatt tgctgtttcc agtagctgaa aaatagaaaa
    15841 tcatagctct gtcttccttt actgccataa aatattctta atggtctgtg cagttacagt
    15901 agtattcatc aatggctgga gtttattgaa aacttttgct ggttcgccaa caagcatata
    15961 tccaacaaat acaccatttt taaataccag cctggaatag gaaaatcttt ctttgtcaat
    16021 catttggtct atttcataag ctgctttttt attatctgtc agattaagcc ccgtatcccc
    16081 tacactaaca actcttgtat tcatcgtcgc aaggatataa ggaaggtctt cacttttata
    16141 aacagcattt cctccggcag cattaatacc tgcaacttgt ccctgttttc cggcaactgc
    16201 ccattgccca taccatctgc cgttaacact tgctacatct cctgctgcat atatatcttt
    16261 aatattcgtc tccatttttt cattaacaat taaacagcgg tccatattga tacctctact
    16321 ttgacaaata gatgtattgg gggcaacacc aactgctaca ataaccagat cggcttcaaa
    16381 gaagctgcca tcagtcatac caactttcat aacatgtcca ttatgatctc ccacaaaacc
    16441 ttcaacagat ttcccgcaca atacggatat tcccagactc tgcactttat ctttaaagat
    16501 ttctgaacct tcttcatcca attgctttgg aagtaatctt ggcatacatt ctatcaatga
    16561 tacctcagta cccatctcag aaattttatg tgccgcttct aaaccaagca gtccaccacc
    16621 aataacgact gcttttctgg cagctaaaag ctctttattt attttgacta tatcatctgt
    16681 tgtccatatg gtatagacgc ctgaaagttc cttaccttca aaagaaggca tgattggcgt
    16741 gcttccagtt gccaaaacca gactgtcata accatactct tttccattta cgctaatttt
    16801 tttagcctta atatctatag ctgtcacctt tgaagaaagc tcaagttgaa tcttgttttt
    16861 ttcaaaccat tcttcgtggt taatttcaag tttatcataa gccagatttt taccaatgta
    16921 atcgcataaa cgaagcctgt aatacggtaa ttgattttct tcaccaaaca tgattatttt
    16981 tgcctctgga tcctgagttc ttgctgcctt tgctgctgaa aagcctgcat tcccgcttcc
    17041 aacaattaaa atttgtctcg aattactcat aacatctgcc ctccaaattt aattgaatct
    17101 ttatttatta tagcacatta aagaaaaaca aaaacaaaat taaaagcctt ccaaaaagaa
    17161 ggctcaaaat ttctttaaag acccataatt atttttttcg ctggtaatat ggatgaggca
    17221 ctcatggaaa actcatcaag accatattca accagaattg gaatggctgt ttcgtcgcca
    17281 gccatttcac cgcacatacc aacccatttg ccatatttgt gggcaccgtc tatggtcatt
    17341 ttgataagtc ttaaaacggc tggatgcatt ggattataga tataagacac tttttcactc
    17401 atgcggtcta cggccagggt atattgaatt aaatcattgg tgccgattga aaaaaagtct
    17461 atatactgag cgagttcatc ggcacaaatg gccgcagccg gaatttcaat cataatgccc
    17521 cattcgatgt cttttgaata tggaatacca ctgacatcta actctttttt acattcttcc
    17581 acgattttca tagcagctaa aaattcctgc aagccagaaa tcataggaaa cataacactt
    17641 agatttccat atactgaagc ccgtaacaga gctcttaatt gtgttttgaa aagttctttt
    17701 tgatccaggc aaagccggat tgccctatag cccaaaaatg gattcatctc ctttggtaaa
    17761 tccatgtatg ggagtgtttt atctcctcca atatccaaag ttcttataac aactttttta
    17821 tctcccattt tttctaaaac aaatctgtag ctttcaaatt gttcttcttc gctgggagca
    17881 gtgtcccggt ccatgtataa aaattctgta cgaaaaagac ctataccatc cccaccattt
    17941 tcaatcactt gatatatatc ctcgggtttt ccgatatttc ctgcaacata aatccttttt
    18001 cctgcttttg tccttgtttc acaggatacc agttttttaa gttcttttaa ttcttcctgg
    18061 tattctttta ttttcttttt atatatttct aattcgtcgt tgcctggatt tataatgacc
    18121 gttccagtaa aaccatcgac aatgaccatg tctccatttt taacagaact acttatatct
    18181 cccatgccta caactgcagg aatttccaga gttcttgcca taatggcaga atgagaggtt
    18241 ctacttccta catcggtcaa aaaagcaata actttatctt tgtctaactg tgctgtatca
    18301 gaaggagtga ggtcatgagc aacgacgaca atgttttctt cttcaatttt aaagtctgtt
    18361 tgattacctg tcagatttgc cattaagcgg tttgtaatgt ctttcagatc tgctgccctt
    18421 tctcttagat attcgtcttc aatggcttca aattgggcaa taaagaaact ggaaacctga
    18481 cttatggctt ttaatgcatt cacatgattg ttttctatct cggaaataat ggcgtcaatg
    18541 aattctctgt cttcaagcat cataatatgc gtttcaaaaa tctctgcttg ttcttctccc
    18601 atggattgtg ctgcttgttc tttcaatttt tccagttgcg ctttactttc ttcgactgcc
    18661 aataagaatt tttctttttc agtctcgata tctgaaatgc tttcatctgt aattttgatt
    18721 tcctgctgag actttaccat aaccttccca attgcatatc cttttgaagc tgcaatacct
    18781 tttttcatct ttacctccac tagcacttat cttcctctgt ttcactattc tcctaatccg
    18841 gattcaatca tttccgttaa agtttttaat gcttcttgtt catcttcccc atcacatact
    18901 aaagtaattt ctgtaccaca aacgatacct gcacttaaaa cgtttaagag cgatttgaca
    18961 ttaattactt tttcctgaaa aagaatcgta atgtccgatt tgaattttga tgctgctttt
    19021 actaagttac ttgccggtct tgcatggagc ccggatttat tcacaacaac aatatcttgc
    19081 tttaccattt acttttcctc cttatccaaa aaatcaagga ctgctgtcaa ctaaccaaac
    19141 ttgacttcta cggttactga gtaccagttt ttattaaaat atgtttcagc atattcaatg
    19201 acttttcctg tttcatcaaa agtagttctt tttacaaaga atgtatccgt tccctttttt
    19261 attttcatga gttttgctat atgactgtca ttgactgttt taatcgaata attttcagtc
    19321 gctcctacag gaataatatt atattctgcc agagttttat aaaaagattt atgttcatga
    19381 attttttgtg cttgctcaag actaagcatc tgactatcca tgaatgaaca ttgataagca
    19441 actggttcat tattaaccaa tcggactctt tttacacaaa tgagggtttg atcaggggct
    19501 aagccaagct tttctgcaat tctttcatct gtaacttctt ctattttgtc cacttgcatg
    19561 tcaattttat agccttgttg taataattca tcactaaaac tgattgaagt taatctgcga
    19621 ttgattggtt cttttgcaac aaaggtacct acaccttgga caccaactaa ataattctca
    19681 ttgattaaat cattaaatgc cttcctaaca gtaatagaag atacattata ttttttctta
    19741 agaacagatt cgctatcaac cttatctcct ggctttagct tccccgattt aatggcattt
    19801 ataatatctg tttttatttg tacatatttt ggtactttat aatccataaa tattaacccc
    19861 ctaatatgtt aatcgcataa ctgacaaata ttataacata ccattgttga actgtaagca
    19921 tgccatcttt ctattcagga aactttgcaa cagctgtctt agtcgtttca acagcgtgat
    19981 ttgccagttc ttctattgat aactcactgt cctctatcat acttaattcc ataattgcca
    20041 tggcgtttaa acctgccagg gtataatatt tccctgcttt acttatgcaa agctctgctg
    20101 ccactctaaa aggagaagca cccaataaat cacatacaaa taatacttca tcatcgtcat
    20161 caaattgttt tactaaatct tcaactctgg ctttaaaacg atctaaatca tcttctttag
    20221 ttaaatcaag aaaatgcata aactcaggaa ctccgacaat catttcaaga ttttttctta
    20281 caccttcggc atatccgcca tgccccatta ctacaactct tttcatattt acaaccatac
    20341 ctttctgcaa cgcattttat aatgccccat ttttactttt ttctgtcttt tcatttttat
    20401 ttgcttttat ggggagggca ggggctttga tacttttaaa gcccctatct tattttaaca
    20461 gatgatacac atctgatgtc tttgaaaaca gaaccaaatt ctataaacta actttatttg
    20521 acaatgatat ttttttaatt acgcataaac ccccgtagcg tatcctaata aagtaagcaa
    20581 acatgcaaga acaaaaatta agcccattaa ttttagcggt gaccattttt tctttgaata
    20641 taagtagtaa acggctaagg ttaatagcaa tggcaaaata ttggggaaga ttgaatttac
    20701 tactttatct gcatcaaaaa tcacttgatt ttccaggatc tgacctgttg ttgcatcaaa
    20761 tacgtctctt acgatttgaa ttggtaaagg aagactaacg aaagatgccg ttaatgcacc
    20821 aacaacgatt aaacccagta cggtcatcgt atctgttaaa gaattaagcc ttcctgatga
    20881 cataaaggtc tgcatacctt caagaccaga tctgtaacct aatttaaaca aataccagct
    20941 taatataaat gttccaatcg ggtaaaccag catatataat acaggaccta accagccagt
    21001 agctaatgat gcttgtgaaa tagacaagca aatggttaaa agaataggta taatggttgc
    21061 aacccataaa gagtcaccaa ttgctgaagt tggaccaatt aaggctactt ttacactgct
    21121 gattaattct ggagtacaat ttccattggc attggcttct tccagaccgc aaacaatacc
    21181 attacaaatg gatcctacct gagattctgt attaaataat gtaatgtgtc tttttaaagc
    21241 ttctgctttt tcttcttttt tatcgtataa ttcatcaata acaggcgcca tagattgccc
    21301 aaaggcaatg cccagcatac tttcagcctg ttgtgaacat ccattccaga aaaaccaatt
    21361 cagaaaagat ctttttaggg ttttttcact aatcttcttc attattgcac ttcccctctc
    21421 ttagcctcta aatttgtaac agcaacataa tacagtacag ctataataat ggctaaaaca
    21481 gccactgcca tggtactaag attgaaataa gttactaata taaagccggc aaggaatatc
    21541 accacatggt attttttctt gaataagaag gaaaggatga ttcccattcc taaagcagcc
    21601 atcattccgc cgaaaacacc taatacggtt gtaagccagc caggaatcaa tgaagcaaag
    21661 tccccctgag ctgaagctgc tgtcatggat gtaaggacaa ttacggcagg aataaaacgg
    21721 catgcaaaac caatcaattg tcctaaaacc acatttggaa taaacatctt tttggtttct
    21781 ccagcttctg catatctttg agcaacacgg tttaatggaa gattaagcgc ataggaaaga
    21841 ttccacataa tttgtcctac taaaccacca ataccagcaa atacaactgc taagccgatc
    21901 acttgtgccg tgtctaagcc ttttccaaat acgagggcag ccgctgtccc aatataggtc
    21961 gcataagata aatcccaggc taccgcacct ccaggggtta cattacccat gtacatgatt
    22021 tgcagggaaa ttccaaccaa tatagcggtc tgcacatctc ccatgattaa ccctacaaca
    22081 aatgatgcaa ctaaaggtct tcccaaggta taccagccaa ttgtattacc aacaattgaa
    22141 ccaattgacc ctaaatagat aaataaggcg ataagaatga tttgataaat ttgcatacat
    22201 gctccccttt tctaattaat agatttcttg aatgcttccc aagtggtttt tgatgttgta
    22261 ggtacttgct gaaagcatac ttcaatacct tttgaagcaa tttcatcgat gattgaaaca
    22321 tctcggtcac ttaagtagaa aataccggtt gcgccactga ctttatgaac tgtatccgga
    22381 cgttttgcga tatttcctaa aataagcatc tccacttgtt ccatttcttc ttttaattct
    22441 tcaagttttt ccggaaatct tacgataact aaccggttta tcggactttc ctctttaagt
    22501 aatttttttg cttcttctgt cgtaacgata tgggtcttat aattttgagg aacccccatc
    22561 gttaaaatgg attttaacat tggatttttt gcactttcgt catctattgc gatgatttcc
    22621 ttaatagata gggccggaca ccaggcaaca atcgtctgcc catgaattaa ccggtcatcg
    22681 acccttatat aagtttttcc catctaacca ctccttttct tctgaaccct tttattctct
    22741 gtttgattac tgttcaatgc attttctcac aaaacctttg ttttctatga ttcttctttc
    22801 tgcctcctct ctggaaactc cagttagaat catgacaatc gcttctttca tagaataatc
    22861 tgtttcttcc agtatctttt cagccgtttt cttgtcacaa cctgtactct ccataacaat
    22921 gcgtttggca cgctctttaa gtttaagatt cgtcggtttc atgtctacca ttagatttcc
    22981 atatgccttc ccgattccta ccatagatgc tgttgaaatc atatttaaga taagtttttg
    23041 acaagtacca gcctttaaac gggtagaacc ggttaatatc tctggtccgg catcaacttc
    23101 aatcgtcaca tctgcgtatt ttgccagttc agaatttttg ttgcatgcta taccaacagc
    23161 ttttgcacca tgttttttgg catgttttaa tgcaccaatg acataagggg ttcttccaga
    23221 agctgcaata ccaacaacaa catccttttc tcctacacta acagcatcca gatctttttc
    23281 agccagttcc tctgaatctt ctgcaccttc aactgcctta ataaaggctt tttctccacc
    23341 tgcgatgata ccaatcactt catccgttgt cgagaaggta ggggcacatt caacagcatc
    23401 caatattcct aatctgccac ttgttcctgc accaatataa ataagtctgc cgttattatt
    23461 gagcgcctta atgaccaatt gcacgcactg ttcgatttgt ggtaaagctt cttttaccgc
    23521 atcaatcacc ttctgatctt cttcattcat gatacgaagt aattccatcg tagacatcgt
    23581 atctaaattc aaagttctcg gattacggct ttcggttttt aaatgatcaa ttcttatatc
    23641 actcatgtct atcaccataa cttattttcg attaaactag cgtacatata tgctactgta
    23701 aagcccgtat tattcgattc aatttgtcga ac
//
LOCUS       DAOSUD010000029        23319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1430_length_23319_cov_25.124570, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000029 DAOSUD010000000
VERSION     DAOSUD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 23319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23319
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_23319_cov_25.124570"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..752)
                     /locus_tag="PLG49_05375"
     CDS             complement(6..752)
                     /locus_tag="PLG49_05375"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOQ16788.1"
                     /translation="MKENIYTIPVIDAFKEDTECPLCHLYMKLEKESIDFMLGPSYME
                     DDIRMETNKIGFCSHHYGKMYEEKNRLGLALMLHTHLEEINKNLSKIISSNKDLNSKS
                     SSIFNVFKTGKNNPSTKNENPISSYLNKVYEGCYICNKIDNTFKRYIDTFFYLWKSDE
                     SFVSLVSEGKGFCLKHFSLLLEKGSQLLNKNQYTHFTQILIPIQQKNLKRIEEELDWF
                     IQKFDYRFKDEPWGNSKDAIPRSLLKIASL"
     gene            857..1732
                     /locus_tag="PLG49_05380"
     CDS             857..1732
                     /locus_tag="PLG49_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOQ16789.1"
                     /translation="MNYRQVKDKIILKDINSFNIEQILECGQCFRFYKISEGHYLIIA
                     KAKVLKILQTDEEVIFYPATIEDFKNLWISYFDLNRDYDIIKQLLAEKDQYLNKAIDY
                     APGIRILNQDPWECLISFIISQNKQIPHIKQVVNHISRRFGQYIDTVDNIDYFAFPTA
                     EELSKAKEEDLRACKAGFRAPYILDACKKIKSGEIDLEKLKYLPYEEAKTTLLNIKGV
                     GPKIADCVLLFAFGKWEAFPVDIWIKRIMESLYFNREISLNEIGHFAREQFGNYAGLA
                     QQYLFYYGRQLKIGK"
     gene            1743..2477
                     /locus_tag="PLG49_05385"
     CDS             1743..2477
                     /locus_tag="PLG49_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOQ16790.1"
                     /translation="MLGTIVNTLAIFFGALLGSIIKKGFNEAFKEIIYHAISLSVIFV
                     GISGAVAHLNDPSSHPILFIISLVIGGLLGQFLDIEGKLERLGNAIEKRMKKDSKNGS
                     ISQGFVTASLIFCVGTMAIIGSLESGLQGVHKTLYAKAVLDGVIAMVLSSTLGTGVAL
                     SAFSVFIYQGSITMLAQWIEPFMTQDMIREISIVGGILIFAIGLNLMEVKKIKVGNLL
                     PSVFIPVIYYLPFIQKFINKLSYFLR"
     gene            2698..2982
                     /gene="groES"
                     /locus_tag="PLG49_05390"
     CDS             2698..2982
                     /gene="groES"
                     /locus_tag="PLG49_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOQ16791.1"
                     /translation="MNLKPLGDRVVLKQLEAEEKTKSGIVLPNQAKERPQEAEVVAVG
                     PGGVVDGKEVKMEVKVGDKVIYSKYAGTEVKIDDVEYIVVRQSDILAIVE"
     gene            3018..4640
                     /gene="groL"
                     /locus_tag="PLG49_05395"
     CDS             3018..4640
                     /gene="groL"
                     /locus_tag="PLG49_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738769.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOQ16792.1"
                     /translation="MAKEIKFGSDARKALEAGVDKLANTVKVTLGPKGRNVVLDKKYG
                     TPLITNDGVTIAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAMIKEG
                     LKNIAAGANPIIIRKGMHKATKKAVEVIKGMSQQINGKSHIAKVAAISAGDEEIGNLI
                     ADAMEKVSNDGVITVEESKTMNTELEIVEGMQFDRGYLSAYMATDMDKMEAVLEDPYI
                     LITDKKISAIQDILPLLEQIVQQGARLLIIAEDVEGEALTTLIVNKLRGTFNCVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISEELGYDLKEATLDMLGRAGTVKVQKENTIIINGA
                     GKKEDIEARIRQIKNQIEETTSEFDKEKLQERLAKLAGGVAVIKVGAATETEMKENKL
                     RIEDALAATRAAVEEGIVPGGGSAYIHAIKEVAALLDSTSGDEKTGVSIVLKALEAPL
                     RQIVTNAGFEDSVVVNKVKELGKNMGFNALTEEYVNMIDAGIIDPTKVTRSALQHANS
                     VASTFLTTEAIVGEIPEKEPPMPGGGAGMGMM"
     gene            4735..5241
                     /locus_tag="PLG49_05400"
     CDS             4735..5241
                     /locus_tag="PLG49_05400"
                     /inference="COORDINATES: protein motif:HMM:NF040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HOQ16793.1"
                     /translation="MGDVFKEQLVAKKRTIKDYILVILIMLVAIVLLLFVIPMLGTFS
                     LLAVALILFGTYWLIQRFDIEYEYILTNDELDVDKIIGKSKRKKLLTVSVKEFEIMAH
                     VNDEKYAHELSNYNKILDYSSGEIKLNTYAAIFNYNNQKVKMIFEPNEEIIKGIFQYI
                     PRKLHIKK"
     gene            5367..6821
                     /gene="guaB"
                     /locus_tag="PLG49_05405"
     CDS             5367..6821
                     /gene="guaB"
                     /locus_tag="PLG49_05405"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ16794.1"
                     /translation="MQKIVKQGVTFDDVLLIPAYSQVLPKDVDLSTQLTKKIKLNIPV
                     MSAGMDTVTEARMAIAMARQGGMGVIHKNMSIEAQADEVDKVKRSEHGVITDPFYLSP
                     EHYVYEANELMAKYRISGVPITEGTKLVGIITNRDLRFETNHNRKIKEVMTKENLITA
                     PEGTTLEEAKEILAKHRIEKLPIVDKSGNLKGLITIKDIEKAILYPNAAKDENGRLLV
                     GAAVGVTSDVIDRVAKLVEAKVDVIVVDTAHGHSQGVIETLKKIKSIYPDLQVIAGNV
                     ATAEATKALIEAGADAVKVGIGPGSICTTRVIAGVGVPQITAIMDCSEAAKPYGIPII
                     ADGGIKYSGDIVKAIAAGASVTMMGGIFAGTDESPGETELYQGRKYKVYRGMGSLAAM
                     EKGSKDRYFQQDAKKLVPEGVEGRVPYKGPVEDTIFQLMGGLRAGMGYCGARNIKELM
                     ENARFVQITSAGLKESHPHDIHITKEAPNYSVEY"
     gene            7005..8324
                     /gene="guaA"
                     /locus_tag="PLG49_05410"
     CDS             7005..8324
                     /gene="guaA"
                     /locus_tag="PLG49_05410"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOQ16795.1"
                     /translation="MNHELILILDFDGKYNQMVAKKVRASNVYCEVNAYDTPVEKIKN
                     KEAKGIIITGDSNNINKKELDSLIKELTTGMSVPILLVIKDFNDDIAGVEKMNAELIH
                     QAEGVQRINQFLFDQCACSGDWNMNDFAKQSIESLKEKIGNKKVLCALSGGVDSSVSA
                     VLVHKAVGKQLTCIFVDTGLMRKNERIEVEKVFREQFDINLICVDAGDRFLGKLKGVT
                     DPERKRKIIGEEFIRVFEEEAKKIGAVDFLVQGTIYPDIIESGSKNTAVIKSHHNVGG
                     LPDVIDFKEILEPLRDLFKDEVRNLGRVLGIPEFLVERQPFPGPGLGVRIIGEITKEK
                     VAILQEADYIFRDEIAKANLNKDISQYFAILTNLRSVGVSGDERTYNYVVALRAICTT
                     DFMNADWARIPYEVLEKASSRIVNEVEHVNRVVYDITPKPPATIEWE"
     gene            8361..9020
                     /gene="deoC"
                     /locus_tag="PLG49_05415"
     CDS             8361..9020
                     /gene="deoC"
                     /locus_tag="PLG49_05415"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ16796.1"
                     /translation="MIDRELAAKIDHTLLRSEAKEEDIRKLCEEAKKYGFATVCVNPC
                     YVTMAKHLLQGTEIGITTVIGFPLGATTSTMKAAETREAIENGATEVDMVINIGALKD
                     KKYEYVLNDIKAVVHAADKKAVVKVIIETGLLTDEEKEKACLLAKEAGADFVKTSTGF
                     LAGGAIKDDIILMRRTVEDTMGVKASGGIKTKGDAEALIKAGANRIGTSASVAIVDNN
                     I"
     regulatory      9118..9218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9349..10737
                     /locus_tag="PLG49_05420"
     CDS             9349..10737
                     /locus_tag="PLG49_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOQ16797.1"
                     /translation="MAQTNANNKPQNNSLLEKLFKLSENKTNVKTEVIAGFTTFMTMA
                     YILAVNPNILGDPAVGMDANALFTTTALAAIIGTLCMALFSNYPFALAPGMGLNAYFI
                     YTVCLKMGYSWQVALTAVLVEGIIFIILTAFNVREAIFNAIPQNLKHAVGAGIGLFIT
                     FIGLQNAKIIVDDPATLLSLGNVTDVVPLLAIIGTIITAILVVKKVKGSILIGIFVTY
                     ILGIIAELTGWYVPNPEAQIYSLIPSGIISMPPSIAPIFFKFDFSQVLSFNFLVVVFA
                     FLFVDIFDTLGTLIGVSSQAGMLDKDGKLPKIKEALLADAIGTTAGAILGTSTVTTYV
                     ESASGVAEGGRTGLTSAVTALFFAIALFFSPIFLAVPSFATAPALIIVGFMMMQSVTK
                     IDFTDAQEGIPSFLTLIFMPFAYSIAEGIVFGIISYTGINAVTGKAKKVHPLMYVLSV
                     VFILKFIFENLV"
     regulatory      10768..10850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            10924..13155
                     /gene="pflB"
                     /locus_tag="PLG49_05425"
     CDS             10924..13155
                     /gene="pflB"
                     /locus_tag="PLG49_05425"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOQ16798.1"
                     /translation="MFKEEWRSFKEGAWQEEINVRDFIQKNYTPYEGNEDFLAGSTER
                     TRALLKRYKELCRLEADWGGVLDIDTSTVSSLLNYSPGYVDKEKEIIVGLQTNRPLKR
                     GVNPFGGIRMTRQACEAYGYKLDKKVEEHFEYRTTHNDGVYRVYTDEMRKARRSGVIT
                     GLPDAYGRGRIIGDYRRIPLYGVDYLIEQKKKDKVEIGKKYMSEENIRLSEELYRQID
                     FLEKLKKMAQMYGYDISRPANDAKEAVQWLYFGYLGAIKEQNGAAMSLGRVSTFLDIY
                     FERDLNEGRLIEIEVQEIIDDFVLKLRMARMLRTPEYNELFAGDPLWITESIGGMGED
                     GRTLVTKTSYRFLNTLYTLEPAPEPNLTVLWSNNLPENFKKFCAKVSIETDSIQYEND
                     DIMREIFGDDYGIACCVSAMKIGKQMQFFGARCNLPKALLMSLNGGVDEISKEQVGPY
                     MEPVSGDVLDYEEVMKRFSYYRQWLCQLYVNTMNIIHYMHDKYAYEKLQMALHDTNVE
                     RFMAFGIAGLSVVADSLSAIKYAKVMPIRDEDGIIIDFKIEGDFPKFGNNDDRVDQIA
                     KELVEDFYNELQKTPAYRNAKHTLSILTITSNVVYGKKTGNTPDGRKKGEPFAPGANP
                     MHNRDVKGALASLNSVAKLPYDSCRDGISCTFSIEPNTLGKTKEEQINNLVGILDGYF
                     AQRSHHLNVNVLNKEKLIDAMEHPEKYPTLTIRVSGYAVNFHKLSREQQEEVINRTFH
                     NKL"
     gene            13175..14731
                     /gene="purH"
                     /locus_tag="PLG49_05430"
     CDS             13175..14731
                     /gene="purH"
                     /locus_tag="PLG49_05430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOQ16799.1"
                     /translation="MKRALISVSDKTGVVEFAEKITELGYEIISTGGTAKALEYAGIK
                     VINISDVTGFPECLDGRVKTLHPNIHAGLLAIRSRKEHMKQLKELDIKPIDLVVVNLY
                     PFKETILKDKVELEEAVENIDIGGPTMLRAAAKNYQDVIVIVDPNDYEKVLEELEKNG
                     DVSEETRFYLSYKVFEHTASYDALIANYFRKKRGDKNFPNTLTLTFEKVQDMRYGENP
                     HQKAAFYKEIDKNPGCLSSAVQLNGKELSFNNINDTNGALELLKEFDEPTVVACKHAN
                     PCGVASADTIYDAYMKAYNADPVSIFGGIVVANREIDKETAIEMNKIFIEIVVAPSYS
                     QEALDILRQKKNIRLLQLDNINAKQPEGAYDLKKVAGGLLIQDVDNLLLPDEELKVVT
                     KRAPSKEEMEDLLFAWRVVKYAKSNGIAIAKNKQSLGIGPGQVNRIWACKQAIDHCKE
                     HLGEEILKGAAMASDAFFPFPDCVEEAAKAGITAIIQPGGSIRDEESIEACDKYGIAM
                     IFTNMRHFKH"
     gene            14978..15691
                     /locus_tag="PLG49_05435"
     CDS             14978..15691
                     /locus_tag="PLG49_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOQ16800.1"
                     /translation="MKFVLDTHCHTVASGHAYSTVQEIAKYASEIGLELVAVTDHSDK
                     LPGGAHLFHFHNLHVIPKKLYGVEILKGAEVNIMDYDGNVDLDEDTLQRLDIVIASFH
                     PPCIKPGNIEENTQAIINAIKNPNIDIIGHPGDPRYPIDIKAVVEAAKEYETLLEVNN
                     NSLKPNGIRSGSTEIIREIMRECVRMDMPFILGSDAHIAFDLGNFKYAEELIAQLDIP
                     EHLIVNRSVKFLKEFLELD"
     gene            15766..16071
                     /locus_tag="PLG49_05440"
     CDS             15766..16071
                     /locus_tag="PLG49_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOQ16801.1"
                     /translation="MNNKIKDESTDRLFEAILLLKNIDECYSFFEDICTINEIKSLAQ
                     RLEVAKMLRANKTYAEITEKTGASTATISRVNRSLIYGSDGYNMILDRLEKENTKEE"
     gene            16160..18370
                     /gene="pcrA"
                     /locus_tag="PLG49_05445"
     CDS             16160..18370
                     /gene="pcrA"
                     /locus_tag="PLG49_05445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOQ16802.1"
                     /translation="MSLLEGLNSKQKEAVLCTEGPLLILAGAGSGKTRALTHRIAYLI
                     KEKGVDPWRILAITFTNKAAREMRERVDLLVGEGAEDIWVSTFHSACVRILRNDIEKL
                     GYNRYFTIYDTDDQKALIKECLKELNYNEKNFPVSGVLGEISRQKDELINPAEYEKRN
                     QGDYRGEKYAKIYKLYQKKLESNNALDFDDIILKTVELFKFHPDVLEYYQNKFQYILV
                     DEYQDTNTAQYKLVSLLAAAHNNICVVGDDDQSIYGWRGANIRNILDFEKDFKNARVV
                     KLEQNYRSTKVILEAANSVIKNNYGRKQKNLWTENEQGDLIELKCCMDEHDEAEYVVR
                     EIDKQAKEGRAYKDFAVLYRTNAQSRVLEEHLVRYSIPYRLFGSIRFYERKEIKDILA
                     YLRVILNPNDDLSLKRIINVPKRGIGATTISKVEEFAAANHMNLYEVIRDRETVSMFG
                     RAAAKLKEFADLIGTLQAEAVQSKSICELMEELLIRTGYLKELEDENTEEARDRIQNI
                     REFVSKAADYDKNAEEISLAGFLEEVALVADIDNYNEGDNAVVLMTLHSAKGLEFPFV
                     FITGLEEGLFPSYMSIQSGREDSIEEERRLYYVGITRAKKKLYLTYANARSFRGERVY
                     YRVSRFIEELPKELIKQEEVKKSSDSLPEMRVKNAIWQQHKAYEAALKKEIPAPQNIS
                     LDFKEGDLVKHFKFGIGTVEKISHAGADYEVTVTFPKYGQKKLMAGLANLKAVH"
     gene            18576..18755
                     /locus_tag="PLG49_05450"
     CDS             18576..18755
                     /locus_tag="PLG49_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16803.1"
                     /translation="MKKENNSEVLKLIELTRKFVDFAETLLKTGKITQEQYQSMTENK
                     YRFLNDMERKKIISK"
     gene            18854..20794
                     /gene="ligA"
                     /locus_tag="PLG49_05455"
     CDS             18854..20794
                     /gene="ligA"
                     /locus_tag="PLG49_05455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ16804.1"
                     /translation="MERMKELIRILNEASKAYYQEDREIMSNKEYDELYDELVELEKK
                     TGIVLADSPTQKVGYTVLSNLPKVRHATPMLSLDKTKDVNKLKSFLKDKKGILSWKMD
                     GLTIVLIYRNGQLAQAITRGNGEIGEDVTNNARVFKNIPLSIPFKNQLVLRGEAVIKY
                     SDFNRINEDLPLEEQYKNPRNLCSGSVRQLNNEITAKRNVYFFAFCLVSAEGEDFKDS
                     KLNQLNWLKSMGFDVVEHKEVYADNIENAVHEFQSKISANDFGSDGLVLTYDSLSYSQ
                     SLGVTAKFPRDSIAFKWADETKETKILYVEWNTSRTGLINPVAVFESVELEGTTVNRA
                     SVHNLSILEELQLGVGDIVRVYKANMIIPQIAENLTKSNNLQIPDKCPVCQGETEIRQ
                     VREMKALYCTNPNCKAQRIKALTHFVSRDAMNIEGFSEASIEKFYNKGFIENFADIYQ
                     LARYEKEIKNMEGFGEKSFNNLMNSIEASKSVDMPNFIYALGLNQVGLSNAKLLCEHF
                     DYDLSKIMNADIEQIKEIHGFGEIIARSVYNYFKNEENRAMVNQLREILSIKGVEKSE
                     NQSLKGLTFVITGSVTHFKNRKELKEKIESLGGKVTDSVTKNTDYLINNDIHSTSSKN
                     KKAKELGIPIIGEDEFLKMANL"
     gene            20979..23285
                     /locus_tag="PLG49_05460"
     CDS             20979..23285
                     /locus_tag="PLG49_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HOQ16805.1"
                     /translation="MKYIKYITCLLTLSIMINFFLVHSVNADARNGMILVYDGQSHIY
                     NEAPIYLVVKGNIIQGDIPPIALNGNTLVPIREVCEAMGAVVEWKTNTKEAYIGMDDS
                     LIILKADSYQAWVNGEYVNIPMPAKIINNKVMVPVRFVAETIGYDVKWFGTERIIMID
                     SKNEDSRQEENHDTTTDIIDHEDTEDIEDIRDITDNEDNGDNKENKDNQTNSDSVISD
                     GTDFKQNVAKLPTKLADDPVVIVSDKEVPTESASSAIESKDYPTTDIISAGVLESVSD
                     GSHFKVTASSPISSLEHMSLEGKIVVDIYNANMKLSNTNMAISNNPFVKSIRTSQFSS
                     NPKVTRVVFDLINQNDYEITMASDRMSFIVRIKKSVITKIELSQNSRGDILTILANAK
                     PSVTVSRLTNPDRLVIDIPNAEANMKAIEKTVKGQYVKAIRTSQFNVDTYRVVLDLEG
                     QPEYEIISVGANGIAVQLITPTFKNIKYLKENSQIAVLKPNKEFNINDITHNDDYMNR
                     IYTLTLPGDYSSVYGQGIFHIGDEKLNYLQISNSLNNKTEFKFAEKTISAVEIYDKGD
                     YFVISLIKPREKYSKIITLDAGHGGSDPGAVGNGLKEKDVNLDILLKLNALLKNSPDI
                     KVYTTRTSDTYPTLQDRSTLANEVEADFFLSIHNNAFNGKNRGTETLYFPTAADNTPQ
                     FSGKKMAQIFQNNLVSRLGTMDRGIKERPGLYVLRTTTMPAVIVEIAFIDNAQDAQLL
                     KSDSFKQEAARALYDGILTIFEQYPTYR"
BASE COUNT         8386 a         3232 c         4749 g         6952 t
ORIGIN      
        1 ttaaattata aactcgcaat tttcagtaaa cttcttggga ttgcatcttt tgaatttccc
       61 catggttcat ctttaaatct gtaatcaaat ttttgtataa accaatcgag ttcctcttca
      121 attcttttta ggtttttttg ttgaatagga ataagaatct gtgtaaaatg agtgtattgg
      181 tttttattta atagctggct gcctttttcc aataataaag aaaaatgttt taagcagaat
      241 ccttttcctt cagaaactaa agaaacaaaa ctttcatcgc ttttccataa gtaaaaaaag
      301 gtatctatat aacgtttaaa ggtattatca attttgttgc aaatgtagca gccctcatag
      361 actttattga gatagcttga aataggattc tcatttttag tggaagggtt gtttttaccc
      421 gttttgaaga cattaaaaat ggaagaactt tttgaattta aatccttatt tgagctaata
      481 attttagaaa gatttttatt aatttcttct aaatgggtat gaagcatcag agcaaggcct
      541 aaacggtttt tttcttcata catttttccg taatgatggg aacaaaaacc tattttattg
      601 gtctccatac ggatatcatc ttccatatat gaaggaccta gcataaaatc aatgctttct
      661 ttttcaagtt tcatataaag gtggcaaaga ggacattctg tatcttcttt aaaagcatcg
      721 ataacaggaa tggtatagat attttctttc attattaacc tccaaaatga ttacagtaat
      781 aaatttatta ttcatcttaa atgacttaag tatagcatgt aaacaacttt gctgcaatag
      841 cagggcaggt gagattatga attatagaca ggtaaaagat aaaatcattc ttaaggatat
      901 aaattctttt aatatagagc aaatattgga atgcggtcaa tgtttcagat tttataagat
      961 ttcagaagga cattatctga taatagcaaa ggctaaagtc ttaaaaattc ttcagacgga
     1021 tgaagaagtc attttttatc ctgctactat tgaagatttt aaaaatttat ggatttctta
     1081 ctttgattta aacagggatt atgatataat taaacaactt ttggcggaaa aggaccaata
     1141 tttgaataag gccattgatt atgcccctgg aatccggatt ttaaatcagg acccctggga
     1201 atgtctgata tcttttatta tttctcaaaa taaacaaatt cctcatatca agcaggttgt
     1261 taatcatatt agcaggaggt ttgggcagta tattgatact gtggacaata tcgattattt
     1321 tgcttttcca actgctgaag agttatcaaa agcaaaagaa gaggatctca gggcatgtaa
     1381 agcaggattc agggctccat atatattaga tgcctgtaaa aaaattaaaa gtggagaaat
     1441 tgatttagaa aaattaaaat acttacctta tgaggaagca aaaacgactt tgcttaatat
     1501 taaaggagta gggcccaaga tagcggattg tgttttactt tttgcttttg gaaaatggga
     1561 agcctttccg gttgatatct ggattaaacg tatcatggag agcttatatt ttaacagaga
     1621 aatttcttta aatgaaatcg gccattttgc aagagaacag ttcgggaatt atgcaggtct
     1681 tgcccagcaa tatttgtttt attatgggag acaacttaag ataggcaagt aggaggggta
     1741 ttatgcttgg taccatcgtc aatactttgg ctattttttt tggagcctta ttaggtagta
     1801 taataaagaa aggttttaat gaagctttta aagaaattat ttatcatgcc atttccttat
     1861 cggtaatttt tgtggggata tcaggagcgg ttgctcattt aaatgacccc tccagtcacc
     1921 ccattttatt tattatcagc ctggttatag gcggcttatt gggacagttt ttagacattg
     1981 aagggaaatt agaaagactt ggaaatgcta tagaaaaaag aatgaagaaa gattcaaaga
     2041 atgggagtat ttctcagggc tttgttacag caagcctgat tttttgtgtt gggaccatgg
     2101 ctattatagg ctctttagaa agcggccttc agggtgtcca taaaacttta tatgccaagg
     2161 ctgtattaga cggggttatt gctatggtat tatcctctac tttagggaca ggtgttgctc
     2221 tttcagcatt ttcggtattt atttatcagg gaagcattac catgctggca caatggatag
     2281 aacctttcat gacgcaggat atgattcgag aaatttccat cgttggagga attttaattt
     2341 ttgcgattgg cctaaatttg atggaagtca aaaagattaa agttggaaac ttgctgcctt
     2401 cagtatttat accggtcatc tattatctac cttttattca aaaatttata aataaattgt
     2461 cttacttttt aagataataa tgaattatta agataagaaa agaaaatcag agaaattaag
     2521 aggataattt aaaaataatg tggtaataat agttaaagct ttattttcgg gaaaaaaagt
     2581 ccttgcataa agatggtagt tttattaata taaattctgt agttagcact caatcagaaa
     2641 gagtgctaac aaacgaagaa atcagaatag aatacatact ttgaaggagg ttaaactatg
     2701 aatttaaaac cattagggga tagggtagta cttaagcagt tagaagctga ggaaaaaaca
     2761 aaatccggta ttgtgcttcc aaatcaggca aaagaaagac ctcaagaagc agaagtagta
     2821 gctgttggac caggcggagt tgtggatggt aaagaagtta aaatggaagt aaaagtaggc
     2881 gataaggtaa tttattctaa gtacgctggc acagaagtaa aaattgacga tgtagaatat
     2941 atcgttgtaa gacaaagtga tatcttagcg attgtagaat aaattaatgg attataataa
     3001 taaaggagga aatgaatatg gcaaaagaga ttaagtttgg ttcagatgct agaaaggcac
     3061 tggaagccgg tgttgataaa ttagccaata ccgtaaaagt aactttagga cctaaaggac
     3121 gtaatgttgt tttagataaa aaatatggaa cccctcttat tacaaatgat ggtgttacta
     3181 ttgcaaaaga aatagagtta gaagatcctt ttgaaaatat gggtgctcaa ctggtaaaag
     3241 aagttgctac caaaaccaat gacgtagcag gagacggaac aaccactgct acagtattgg
     3301 ctcaggcaat gattaaagaa ggacttaaaa atattgctgc aggtgcaaat ccaatcatca
     3361 ttagaaaagg aatgcacaaa gcaaccaaaa aagctgttga agttattaaa ggcatgagcc
     3421 aacaaatcaa cggaaaatct catattgcaa aagttgctgc aatttctgct ggcgacgaag
     3481 aaattggtaa cttaattgca gatgcaatgg aaaaggtttc caatgacggt gtaattactg
     3541 tagaagaatc taaaactatg aatactgaat tagaaatcgt tgaaggtatg caatttgaca
     3601 gaggatactt atcagcttac atggcaaccg atatggacaa gatggaagcc gttcttgaag
     3661 atccgtatat cttaattaca gataagaaga tttctgctat tcaagatatt cttccattat
     3721 tagaacaaat cgttcaacaa ggtgcaagat tattaattat tgctgaagac gtagaaggag
     3781 aagcgctgac gactttaatc gttaataaat taagaggtac ctttaactgt gtagctgtta
     3841 aagcaccagg atttggagac agaagaaaag aaatgttaag agatattgct atcttaacgg
     3901 gtggtcaggt gatttctgaa gaactgggtt atgacttaaa agaagctaca ttagatatgc
     3961 ttggcagagc aggtaccgta aaagttcaaa aagaaaatac cattatcatt aatggtgcag
     4021 gtaagaaaga agatattgaa gctagaatca gacaaatcaa gaatcaaatc gaagaaacca
     4081 cttctgagtt tgacaaagaa aaattacaag aaagattagc aaaattagcg ggtggcgtag
     4141 cagtaattaa agtaggcgct gctacagaaa cagaaatgaa agaaaataaa ctccgcattg
     4201 aagacgcttt ggctgcaaca agagctgctg ttgaagaagg aattgttcct ggtggaggat
     4261 cagcatatat tcatgcaatt aaagaagttg ctgcgttact tgacagcact tctggtgatg
     4321 aaaagacagg cgttagcatt gtacttaaag ctttagaagc tccacttcgt caaattgtta
     4381 ctaatgcagg atttgaagat tctgttgttg taaataaagt aaaagaatta ggaaagaata
     4441 tgggcttcaa tgcgcttaca gaagaatatg tcaatatgat tgatgctgga atcattgacc
     4501 caacgaaggt aaccagaagc gcacttcaac atgcgaattc agttgcatca accttcttaa
     4561 caactgaagc aattgttgga gaaattcctg aaaaagaacc tccaatgcct ggcggaggcg
     4621 caggaatggg aatgatgtaa tttaaaacag cttgccaaaa aggcaagctg ttttttcttg
     4681 atatttaaat ttgtatatga taggatataa caaagaaaaa aagggaggat ggatatggga
     4741 gatgttttta aagaacagct cgtagcaaaa aagcggacga ttaaagatta catcttagtt
     4801 attttgatta tgcttgttgc aatcgtttta ttattatttg tcatacctat gttaggaact
     4861 ttttctttat tggcagttgc ccttattttg tttggaacat attggctgat tcaaagattt
     4921 gatattgaat atgaatatat attgaccaat gatgaattag atgtagataa gattataggt
     4981 aaaagtaagc gtaaaaaact tttaacggtt tcggtaaaag aatttgaaat catggctcat
     5041 gttaacgatg aaaaatatgc tcatgaattg agtaattata ataaaatatt agattacagc
     5101 agtggagaaa taaaactcaa tacttatgct gccatcttta actataataa tcaaaaggta
     5161 aaaatgattt ttgagcctaa tgaagaaatt ataaaaggaa tttttcaata tattcctaga
     5221 aaattgcata taaaaaaata aatttttggg cacattaaat gtgcctgtta ttattaatca
     5281 attttattat taaaagtttt aataaatagt tgacaagggg aaaattattt gataaacttt
     5341 gcgaaaaatt aaggagaggt tgagaaatgc aaaaaattgt aaagcaagga gtaacatttg
     5401 atgacgtatt attaatacct gcctattctc aggttcttcc taaagatgtg gatctatcaa
     5461 cacagcttac aaaaaaaatt aagttaaata ttcctgtaat gagtgctggt atggatacgg
     5521 ttacagaagc cagaatggct attgccatgg cacgtcaggg tggcatgggt gttattcata
     5581 aaaatatgtc tattgaagcg caagcagatg aagtggacaa agtaaaacgt tctgaacatg
     5641 gcgttattac agatcctttt tacttatccc cagagcatta tgtatatgaa gctaatgagt
     5701 taatggcaaa atatcgtatt tcaggggtgc ccattactga agggacaaag ttggtaggta
     5761 ttattaccaa tagagattta cgctttgaaa ccaatcacaa ccgaaaaatt aaagaggtta
     5821 tgacaaagga aaatttaatt acggctcctg aagggacgac attagaagaa gcaaaagaaa
     5881 ttttagcaaa gcatagaatt gagaaattac ctatcgtcga taaatccgga aatctaaaag
     5941 gtcttattac cataaaagat attgaaaaag ccattttata ccctaatgct gcaaaagacg
     6001 aaaacggaag attattagtt ggtgcagcgg ttggtgttac aagtgacgtc attgacagag
     6061 tagcaaaact ggtagaggct aaggtagatg tcattgttgt agacacagct catggccact
     6121 ctcagggagt tattgagacc ctaaagaaga taaaaagtat ttatccggat ttacaggtta
     6181 ttgcaggtaa tgtggctaca gctgaagcaa ccaaagcttt aattgaagca ggagcagacg
     6241 cagtaaaggt tggcattggt cctggttcta tttgtacgac aagagtgatt gcaggcgttg
     6301 gtgttcctca aattactgcg attatggatt gttctgaagc agcaaaacct tatggaattc
     6361 caataatagc agatggaggc attaaatatt caggagatat tgttaaggca attgcagctg
     6421 gagcaagtgt gactatgatg ggtggcatat tcgccggaac tgatgaaagt cctggtgaaa
     6481 ctgaattata ccaaggcaga aaatataaag tatatagagg gatgggttct ttagcggcca
     6541 tggaaaaagg aagcaaagat agatatttcc aacaagatgc taaaaaactt gtacctgaag
     6601 gtgtcgaagg aagagtgcct tataaaggac ctgttgaaga tacaatattc caactcatgg
     6661 gaggacttcg ggcaggaatg ggatactgtg gtgcaagaaa cattaaagaa ttaatggaaa
     6721 atgcaagatt tgtacaaatt accagtgcag gtttaaaaga aagtcatcct catgacatac
     6781 atatcaccaa agaagctcca aactatagtg tagaatatta atattaaaaa taatccggat
     6841 tgaccaaagt ctgtccggat ttatatatta tgcgattatt ttttgactat ttaataaaat
     6901 gtttgacagc tttcttttat ctcatgtatt ataattatat tctatatttt taaatagttt
     6961 agatttagag atatttaaat ggattattat gggaggatac aaatatgaac catgaactta
     7021 ttcttatatt agatttcgat ggtaaatata atcaaatggt agctaaaaaa gtcagggcat
     7081 cgaatgtata ttgtgaagtc aatgcttatg atacacctgt tgaaaaaata aaaaataagg
     7141 aagcaaaagg tattattatt acaggagatt caaataatat taacaaaaaa gaattggata
     7201 gtttaataaa agaacttaca acaggcatgt ctgtacctat tttacttgtg attaaagatt
     7261 tcaatgacga tatagcaggc gtagaaaaaa tgaatgcaga actcattcat caagcagaag
     7321 gcgttcaacg catcaatcaa tttctgtttg atcaatgtgc ctgcagtggc gattggaata
     7381 tgaatgattt tgcaaagcaa tctatcgaaa gcctgaaaga aaaaatagga aacaagaaag
     7441 tactttgtgc tctttctggc ggggttgact catccgtttc tgccgtactg gttcataaag
     7501 ccgttggtaa gcagttgaca tgtatttttg tagacactgg tcttatgaga aaaaatgaaa
     7561 gaatagaagt tgaaaaagta tttagagaac aatttgatat caatttgatt tgtgtggatg
     7621 caggggatag atttttagga aaattaaaag gagtaaccga tccggaaaga aaaagaaaga
     7681 ttattggtga agaatttata agggtttttg aagaagaagc taaaaaaatt ggagctgtgg
     7741 acttcctggt acaaggaacg atttatccgg acattattga aagcggcagc aaaaatacag
     7801 ctgttatcaa aagtcatcac aatgtcggag gacttccgga tgttattgat tttaaagaaa
     7861 ttcttgaacc attaagagac ttatttaagg atgaggtaag aaatttagga cgagttcttg
     7921 gaatacctga atttttagta gaaagacagc cattcccagg tccaggttta ggcgtaagaa
     7981 ttattggaga aattacaaaa gaaaaagttg ctattttgca agaggcagat tatatcttcc
     8041 gtgatgaaat agcaaaagca aatcttaata aagatatcag ccagtatttt gcaattttga
     8101 cgaatttgcg ttctgtaggt gtttcaggag atgagagaac gtataattat gtcgttgccc
     8161 taagagcgat ttgcacaacg gattttatga atgctgattg ggcaagaata ccttatgaag
     8221 tattagaaaa agcttcatcc agaattgtca atgaagtaga gcatgtcaac agggtggttt
     8281 atgacattac acctaagcca ccagcaacga tcgaatggga ataaaattaa attttattta
     8341 taaaaattag gaggaatata atgattgacc gagaattggc agctaagatt gaccacactt
     8401 tgttaagatc tgaagcgaaa gaagaagata ttagaaaact ctgtgaagag gcaaaaaaat
     8461 acggatttgc gacggtatgt gtaaatcctt gttatgttac catggcaaag catttgcttc
     8521 aaggaacaga gattggcatt acaaccgtta taggatttcc tttaggagca accaccagta
     8581 caatgaaagc agctgagacc agagaagcca tagaaaatgg tgcaacagag gtggatatgg
     8641 tcattaatat tggggcttta aaagataaga aatatgaata tgtcctcaat gatattaagg
     8701 ctgttgttca tgctgctgat aaaaaagctg tcgtgaaagt gattattgaa acgggtcttt
     8761 taacggatga agaaaaagaa aaggcttgcc ttttagccaa agaagccggg gctgattttg
     8821 ttaagacttc tacaggcttc ttagctggag gggcaataaa ggatgatatt attttaatga
     8881 gaagaaccgt agaagatacc atgggggtaa aggcatccgg tggaataaag accaaaggag
     8941 atgccgaagc tttaataaaa gctggagcca accgtatagg aacgtctgct tctgttgcca
     9001 ttgttgacaa taatatataa aattgatttc gaacgttcag tttgtttttg gataaaaagt
     9061 tcgtaatact gttgacaata tacaagatta tttgataaaa tacatttatc agtaaggttt
     9121 ttaaaaggtt tattcactta tataagtctg gaaatatggt ctggatgttt ctaccgacaa
     9181 ccgtaaattg tctggctata ggtgaaatga acgacttaat gattttgcat tattaagttc
     9241 gttccttcat ctttgaggga acgaattttt taatggatca gggatatatt catcgtttat
     9301 ctcatcctta tctctttcaa ttttaatctt taagggagga agtcaataat ggcacaaaca
     9361 aatgcgaaca ataagcccca aaacaatagt ttattagaaa agctctttaa gttaagtgag
     9421 aacaagacca atgttaagac ggaagtgatt gcaggcttta caacatttat gaccatggcg
     9481 tatattttgg ctgtaaatcc taatattcta ggtgatccgg ctgttggtat ggatgccaat
     9541 gccttattta ccacgacagc tttggcagca attattggaa ccttgtgtat ggcacttttt
     9601 tcaaattatc cctttgccct ggctccaggc atgggtttga acgcatattt tatttataca
     9661 gtttgtctta aaatgggtta tagttggcaa gtggcattaa cagcggtttt agtagaaggt
     9721 attattttta tcattttaac tgcatttaat gtaagagaag caatttttaa tgctattcct
     9781 caaaatttaa agcatgcagt aggagcagga attggtttat ttattacttt tattggttta
     9841 caaaatgcaa agattattgt agatgatccg gcgactcttt tatctttagg caatgtaact
     9901 gatgtagtac ctttgttagc aattatcgga acaattatta ctgcaatttt agttgtgaaa
     9961 aaagttaaag gttcaatttt gatcggaata tttgttactt acattttggg tattattgca
    10021 gagttaactg gatggtatgt tcctaatcca gaggctcaaa tctatagttt aataccttca
    10081 ggcattatat caatgcctcc aagtatcgca ccaattttct ttaaatttga tttttcacaa
    10141 gtgctttcat ttaacttttt agttgttgtt tttgcattct tatttgttga tatatttgat
    10201 actttaggaa cattaattgg cgtatccagc caggcgggca tgttagataa agacggaaaa
    10261 cttcccaaaa ttaaagaagc cttattggca gatgctatag gaacaacggc tggcgcaatt
    10321 cttggtactt ctacagtaac cacttatgtt gaaagtgctt ctggagtagc tgaaggagga
    10381 agaacaggtt taacttctgc tgttactgca ctcttttttg ccattgcatt attcttttca
    10441 ccaattttcc ttgcagtacc ttcttttgca actgcaccag cacttatcat cgtaggcttt
    10501 atgatgatgc agtctgtaac aaaaatagac tttacagatg ctcaagaagg aattccttct
    10561 ttcttaacct taatctttat gccttttgca tatagcattg cagaaggaat tgtatttggt
    10621 ataatatctt atacaggaat taatgctgta acaggaaaag caaagaaagt tcatccacta
    10681 atgtacgttt tgtcagtagt atttatctta aaatttattt ttgaaaattt agtttaatat
    10741 tattcatgca caataatgta ttaaataaat gccttgtatg actggcggat aatgggaata
    10801 aacccatagg gaatacaagg taaaatcagc cgaccgcctg ggcagacaga attttgattt
    10861 ctgtttgttc aggtggtttt ttattttccg tcatacattt tacaatgtag gaggaggatg
    10921 agaatgttta aagaggaatg gagatctttt aaagaaggtg cttggcaaga ggagataaac
    10981 gtaagagact ttattcaaaa aaattatact ccctatgaag gcaatgaaga ctttttggct
    11041 ggaagtacag aaagaacaag ggcattatta aaaagataca aggagctttg ccgccttgaa
    11101 gcagactggg gaggtgtttt agacattgat acttctacgg tatcctcttt actaaattat
    11161 tctcccggat atgtcgataa agaaaaagag attatcgttg gattgcagac caacaggcct
    11221 ctaaaaagag gagttaatcc ctttggcgga atccgtatga caagacaggc gtgtgaagct
    11281 tatggttata aactggataa aaaagtagaa gaacattttg aatatagaac aacccataat
    11341 gatggagttt acagagttta taccgatgaa atgagaaagg ccaggagaag tggtgttatt
    11401 acaggcttac ctgatgctta tggacggggc agaattattg gagattatag aagaattcca
    11461 ctttatggtg tagattatct gattgaacaa aagaaaaagg ataaagtcga aattgggaaa
    11521 aagtacatga gtgaggaaaa tatccggctt tcagaagaac tttacaggca aatagatttt
    11581 ttggaaaaac tcaagaaaat ggcccagatg tatggctatg atatttccag gcctgccaat
    11641 gatgcaaaag aggcagtaca gtggctttat tttggttatt taggtgctat taaggaacaa
    11701 aatggggcag ccatgtctct tggacgggta agcacctttt tagacattta ttttgaaaga
    11761 gatttaaatg aaggtagatt aatagaaata gaagtacagg aaatcataga cgattttgta
    11821 cttaagttga gaatggcaag aatgctgagg accccggaat acaatgaatt gtttgcagga
    11881 gatcctctct ggataacaga atctataggc ggtatgggtg aagatggtcg gactttagtg
    11941 actaaaactt cttataggtt tttaaataca ctttataccc tggaacctgc accagaaccc
    12001 aatttaacgg ttctctggtc caataatctt ccggagaatt ttaaaaaatt ttgtgcaaaa
    12061 gtatctattg aaacggattc catacaatat gaaaatgatg atataatgag agaaatattt
    12121 ggagatgatt atggcattgc ctgctgcgta tcagccatga aaattggaaa gcagatgcag
    12181 ttttttgggg cacgctgtaa ccttcctaag gccttgctga tgagtctgaa tggaggcgta
    12241 gatgaaatca gcaaagaaca agtagggcct tatatggaac ctgtttcagg ggatgttctt
    12301 gattatgaag aagtcatgaa gcgtttttct tactatcgtc aatggctttg ccaactttat
    12361 gtgaatacga tgaatatcat tcattatatg cacgataaat atgcttacga aaagcttcag
    12421 atggcccttc atgatactaa tgtggaacgt tttatggctt ttggaattgc gggtctttca
    12481 gtcgttgccg attctttaag tgctataaaa tatgctaaag ttatgcccat tcgggatgag
    12541 gatggtatta ttatagattt taaaatagaa ggagattttc caaagttcgg aaataacgat
    12601 gacagggttg accaaattgc aaaggaatta gtggaagatt tttataatga attgcaaaaa
    12661 acgcctgcat atcgtaatgc aaaacataca ttatcgattt taaccattac atccaatgtg
    12721 gtttatggaa agaagaccgg gaatactcca gatggaagaa aaaaaggaga accttttgca
    12781 cctggcgcaa atccaatgca taacagagat gtaaagggag ctttggcttc tttaaattct
    12841 gtagcaaaac taccttatga tagttgcagg gatggtattt catgtacctt ttcaatagag
    12901 ccaaatactt taggaaagac gaaagaagaa caaattaata atttagtggg aattttagac
    12961 ggttattttg ctcaaagatc tcatcatctg aatgtcaatg tcctgaacaa ggaaaagcta
    13021 atagatgcta tggaacatcc ggaaaagtat cctactttga ctataagggt atctggttat
    13081 gctgtaaatt tccataaact cagcagagaa caacaagaag aggtaatcaa tagaacattt
    13141 cataataaat tataaaaatg aaaggagaat aaagatgaag cgcgctttaa tcagtgtatc
    13201 cgacaaaaca ggggttgtag aatttgcaga aaaaataact gagttaggct atgaaattat
    13261 atctacaggg gggacagcta aagcccttga atatgctgga atcaaagtca ttaacattag
    13321 cgatgtgaca gggtttccag aatgcttaga tggaagggtt aaaacgctcc atcctaacat
    13381 tcacgcgggt cttttagcta taagaagcag aaaagaacat atgaagcaac tgaaagagtt
    13441 agatataaag cccattgatt tagtagtcgt taatttatat cctttcaaag aaaccatatt
    13501 aaaagataag gtagaattag aggaagctgt ggaaaatata gatattggcg gacctaccat
    13561 gcttcgtgct gcagcaaaaa attatcagga tgttattgtt attgtagacc ctaatgatta
    13621 tgaaaaagtt cttgaagaat tagaaaagaa tggggatgta tcagaagaaa caagatttta
    13681 tttatcttat aaagtctttg agcatacagc aagttatgat gccctcatag caaattattt
    13741 cagaaagaaa agaggagaca aaaacttccc caatacgctt actttaactt ttgaaaaagt
    13801 tcaggatatg agatatggtg aaaatccgca tcaaaaagca gctttttaca aagaaatcga
    13861 taaaaaccct ggctgtctaa gctcagctgt acagcttaac ggaaaagagc tctcctttaa
    13921 taatataaac gataccaatg gcgcattgga acttttgaaa gaatttgacg aacctacagt
    13981 tgttgcatgt aaacatgcta atccatgtgg ggtagccagt gcggatacaa tttatgatgc
    14041 atatatgaag gcttataatg ccgatccggt ttcaatattt ggtggtattg tcgttgctaa
    14101 cagagaaatt gataaagaaa cggcaataga aatgaataag atatttatag aaatcgttgt
    14161 tgcaccttct tattctcagg aagcattaga tatcttaagg caaaagaaaa atattcgtct
    14221 tcttcagtta gacaatatta acgcaaagca accagaaggc gcttatgact taaagaaagt
    14281 ggcaggcgga ctgcttattc aggatgtaga caatttactt ttacctgatg aggaattaaa
    14341 agttgttacc aaaagagctc ccagtaaaga agaaatggaa gacttattat tcgcatggag
    14401 agttgttaag tatgcaaaat ctaatggtat agccattgca aagaataaac agtctttagg
    14461 tattggaccc ggacaagtaa atcgaatttg ggcttgtaaa caagctattg accattgtaa
    14521 ggaacatctg ggagaagaaa ttttaaaagg tgctgctatg gcttcggatg catttttccc
    14581 attccctgac tgtgtagaag aagcagctaa ggcagggata acggctatca tacagcctgg
    14641 tggctccata agagatgagg aatcaataga agcctgtgat aaatatggca tagcgatgat
    14701 atttaccaat atgagacact ttaaacatta atcctacata ataaaaaata accgccaaaa
    14761 aatctttggc ggttattttt tgttaaaaga gaaattaact ttaatatcat tccaataaaa
    14821 agttttttgt ggggaaaaat aagaaagaaa ttgtctcttt tttgatgaca gataaaacga
    14881 gtttaattgc aattatttat aaatgtgtta caatagtcaa ggaagataat aaaaaaatta
    14941 aagtattttt tatcagaatt caaggagatg gacatcaatg aaatttgttt tagacactca
    15001 ttgccataca gtggcaagtg gtcatgctta cagtactgta caagaaatag ccaaatatgc
    15061 ttctgaaatt gggctggagc ttgttgctgt tacagatcat tcggacaagc tgccaggagg
    15121 agcacatctt tttcattttc acaatttaca tgtcattcct aaaaaattat atggcgtaga
    15181 aattttaaag ggtgcagaag ttaatattat ggattatgac ggaaatgtgg atttggatga
    15241 agatacgctg caaaggcttg atattgttat agcaagtttt catcctccct gcataaaacc
    15301 aggaaatatc gaagaaaata cccaggccat aattaatgcc attaaaaatc ctaatataga
    15361 tataataggg catcctggtg atccccgcta tccgatagat ataaaagcag tcgtggaagc
    15421 agctaaggaa tatgagactt tactggaggt caataataat tctttaaaac ctaacggtat
    15481 tagatccgga agtactgaaa ttatcagaga aatcatgagg gaatgtgtaa ggatggacat
    15541 gccttttatt ctgggcagtg acgcccatat tgctttcgat ttaggaaatt ttaaatatgc
    15601 tgaagagctt attgcacagt tagacattcc tgaacattta attgtcaacc gttctgtgaa
    15661 atttttaaag gaatttctgg aacttgacta gcaggacttt catactgtaa aatattaaag
    15721 gaaatgatga ttactgacta cgtatcaggt aggtgaattg tatcaatgaa taataaaatt
    15781 aaagatgaaa gtactgaccg tttgtttgaa gcaatcttac ttcttaagaa tatagatgaa
    15841 tgttattctt tttttgaaga catatgtaca atcaatgaaa ttaaatcttt agcacagaga
    15901 ttagaagtgg caaaaatgtt aagagccaat aaaacatatg ctgagattac agaaaaaaca
    15961 ggtgcttcaa cggcaaccat aagtagggtt aaccgctcat taatttatgg ttcagatgga
    16021 tataacatga ttcttgacag attagaaaaa gaaaatacaa aggaagaata ataaagaaat
    16081 aaaaaacaaa aatataaaca aattatcctt tccattaagt gatggaaagg tttttgttta
    16141 ctggaaaagg agaattacta tgtctttact ggaaggatta aattctaaac aaaaagaagc
    16201 tgtcttatgc actgaagggc ctttattgat tcttgcaggg gcagggtcag gaaaaaccag
    16261 agctttaacg cacagaattg cctaccttat taaagaaaag ggagtagatc catggagaat
    16321 tcttgcgatt acttttacca ataaagcagc cagggaaatg cgtgaaagag ttgatctttt
    16381 agttggtgaa ggcgcagaag atatatgggt gagtactttt cattctgctt gtgtgagaat
    16441 tcttagaaat gatatcgaaa agctgggcta caaccgctat tttacaattt atgataccga
    16501 cgatcaaaag gccttgataa aagagtgctt aaaagaatta aattacaacg aaaaaaattt
    16561 cccagtaagc ggagtattag gagaaattag caggcaaaaa gatgaactta taaatcctgc
    16621 agaatatgaa aaaagaaacc agggagacta tcggggagaa aaatatgcaa aaatctataa
    16681 gctttaccag aaaaaattag aaagtaataa tgctttggat tttgatgata ttatattaaa
    16741 aacggtagaa ttgtttaaat ttcatccgga tgtattagaa tattatcaaa ataaatttca
    16801 atatatatta gtagacgaat accaggatac aaatacggct cagtataagt tagtcagttt
    16861 actggcagct gctcacaata atatttgtgt tgttggtgac gatgatcaaa gcatttatgg
    16921 ttggcgggga gctaatattc gaaatattct tgattttgaa aaagatttta agaacgccag
    16981 ggtggttaaa ctagaacaaa attaccgctc cacaaaagtg attttagaag ctgctaattc
    17041 agttataaaa aataattacg gaagaaaaca aaaaaatctt tggacagaaa atgaacaagg
    17101 tgatttaatc gagttaaagt gttgtatgga tgagcatgac gaagcggaat atgtagtaag
    17161 agaaatcgat aagcaagcta aagaaggaag agcttataag gattttgcag ttttatatcg
    17221 aaccaatgcg cagtcccggg tattagaaga acatcttgta aggtacagca tcccttacag
    17281 attgtttgga agcatcaggt tttatgaaag aaaggaaatt aaggatatct tagcttatct
    17341 cagagtgatt ttaaatccca atgatgattt atctttaaaa agaattatta atgttcctaa
    17401 aaggggtata ggggctacta cgatttctaa agttgaagaa tttgctgcag ccaatcatat
    17461 gaacttatat gaggtaataa gggatagaga aaccgtatct atgtttggtc gtgcagcagc
    17521 taaattaaaa gaatttgctg acttaatagg gactctgcaa gctgaagcgg ttcaatcaaa
    17581 gagtatctgt gaactgatgg aagaactttt aatacgtaca ggatatttga aagaattaga
    17641 agatgaaaat acagaagaag caagagacag aatacaaaat atcagagaat ttgtatctaa
    17701 ggctgcagat tacgataaaa atgcagaaga aattagtctg gcaggttttt tggaagaggt
    17761 tgcgctggta gcagatatcg acaattacaa tgaaggtgac aatgcagttg tacttatgac
    17821 cttgcacagt gcaaagggtt tagaatttcc atttgttttt attacaggac ttgaagaagg
    17881 attatttcca agttatatga gtattcagtc aggaagagaa gacagtatag aagaagaaag
    17941 aagactttat tatgttggta ttacccgggc aaaaaagaaa ttatatttaa cttatgccaa
    18001 tgccagaagc ttcagaggag aaagagtata ctacagagta tccagattta ttgaagaatt
    18061 accaaaagag cttatcaaac aggaagaagt aaaaaagtca tcagattctt tgccagaaat
    18121 gagagtaaaa aatgcaatat ggcaacagca taaagcttat gaggctgctt taaagaaaga
    18181 aattcctgca cctcaaaata taagtctgga ttttaaagaa ggggatttag tgaaacattt
    18241 taagtttggt ataggaactg tagaaaaaat aagccatgca ggtgctgact atgaagttac
    18301 agtaactttc cccaaatacg gtcagaagaa attaatggca ggtcttgcaa atctaaaagc
    18361 agtacattaa aaaagatatc aagagaaatt taaagaaatt ttaagaaaac gaaagaaaag
    18421 taaaaataga gggagatagc tgtgatattt gccgtattta aactcgtgga aaaattgtaa
    18481 taaaaagtaa attatgatat aatgctctca taattatgca tcgtatttaa ttttgagttt
    18541 aaatacaaaa aatgggaatg ggaaagtggg atgtcatgaa aaaagagaac aattcagagg
    18601 tattgaagtt aattgaactg acaagaaaat ttgtagattt tgcagagacc cttcttaaaa
    18661 ctggtaaaat cactcaggaa caatatcaaa gcatgacaga aaataagtat cgctttctta
    18721 atgacatgga acgaaagaaa attattagta agtgaaaaca ggcatatttg tctgtttttt
    18781 tactttctat tataattata gtattataat gatagctttt tacttttcac tgataattag
    18841 ggggagaact atgatggagc ggatgaaaga actaatcagg attttaaacg aagcttcaaa
    18901 ggcttattac caggaagaca gggaaatcat gtccaataaa gaatatgacg agctttacga
    18961 cgaattagta gaattagaaa agaaaactgg cattgtcctt gcagacagtc ctactcaaaa
    19021 agtgggttat actgttctca gtaacttacc taaagtgcgt catgcaacgc caatgctttc
    19081 attagataaa actaaggatg tcaataaatt aaagagcttt ttaaaagata aaaaaggtat
    19141 actttcatgg aaaatggatg gtttaaccat tgtgctgatt tatagaaatg gacaattagc
    19201 ccaggccata acccgtggga atggagaaat aggggaagat gtcaccaata atgccagggt
    19261 atttaaaaac attcccctta gtataccatt taaaaaccaa ttggttttaa gaggggaagc
    19321 tgttataaag tactcggatt ttaatcgaat aaacgaagat ctgcctttag aagagcaata
    19381 taaaaatccc aggaatttat gcagtggcag cgtaagacag cttaataatg aaataacagc
    19441 aaaaagaaat gtatatttct ttgctttttg cctggtttct gcagaaggag aagattttaa
    19501 ggattctaaa ttaaatcagt taaactggct taaaagcatg ggattcgatg tggtggaaca
    19561 taaagaagtc tatgcagata atatagaaaa tgctgttcat gaatttcaaa gtaaaatttc
    19621 agcaaatgat tttggctcgg atggtttggt tttaacttat gacagtttgt cctattccca
    19681 gtcattggga gtcactgcta aattcccaag agattcaatt gcttttaaat gggcggatga
    19741 aacaaaagag actaaaattt tatacgtaga atggaatact tcaaggactg gtttaattaa
    19801 tccagttgcc gtatttgaaa gtgtagaatt agaagggacc acagtaaacc gggccagtgt
    19861 acataattta agtattttgg aagaactgca gttgggtgtg ggagatattg taagagtata
    19921 taaagccaat atgattattc cacaaatcgc agaaaacctt acaaaaagca ataatcttca
    19981 gatacctgat aagtgtcctg tctgccaggg agaaacagaa atcagacaag taagagaaat
    20041 gaaggcctta tactgtacga atccaaattg taaggcacaa agaataaaag ccctaactca
    20101 ttttgtatcc agagatgcta tgaatattga aggtttttct gaagcaagca ttgagaagtt
    20161 ttacaataag ggttttattg aaaattttgc agatatctac caattagcac gttatgaaaa
    20221 agaaattaaa aatatggaag gttttggaga aaaatccttt aataatttga tgaattctat
    20281 tgaagcgtca aaaagtgtgg acatgcctaa ttttatatac gctttaggtc taaaccaggt
    20341 tggtttaagt aatgcaaaat tattatgcga acattttgat tatgatttat caaaaattat
    20401 gaatgcagat atagagcaaa ttaaagaaat acatggcttt ggagagatta ttgctcgttc
    20461 agtctacaat tattttaaaa atgaagaaaa cagagcaatg gttaatcagt taagggagat
    20521 tttaagtatt aaaggcgtag aaaaaagtga aaatcaaagc ctcaaaggtt tgacttttgt
    20581 tattacaggc agcgtaaccc attttaaaaa cagaaaagaa ttaaaagaaa aaatagaatc
    20641 tttaggtgga aaagtaacag attccgttac aaagaataca gattatttaa taaataatga
    20701 tattcattca acttcttcaa aaaataaaaa ggcaaaagaa ttaggaatac caattatagg
    20761 ggaagatgaa tttttaaaaa tggccaatct ttaaccggag gaaatatctc cggtttttct
    20821 tttatagaga gaaaatagcg aaatattaca tagttgttac aaaaatattt caaattctat
    20881 attggcttta ataagtttac atcttctgct ataattttat ttagtgagag aaaagttgac
    20941 attttgggct gagcataatg gataatggag gggaaaagat gaaatatatc aaatatatta
    21001 catgtttatt aacgttgtcg ataatgatta atttcttttt agtacatagt gtgaatgcag
    21061 acgctcgtaa tggaatgata cttgtatatg atggacaaag ccatatttat aatgaagcgc
    21121 ctatttattt agtcgtaaaa ggcaacatta tccaaggaga tatccctcca attgcattaa
    21181 acggtaatac attagtacct attcgtgaag tttgtgaagc aatgggtgct gttgtggaat
    21241 ggaagacaaa taccaaggaa gcctatattg gaatggacga ttcattaatt attttaaaag
    21301 ccgacagcta tcaggcttgg gttaatgggg aatatgtaaa tattccaatg cctgcaaaaa
    21361 ttattaataa taaagtgatg gttccggtaa gatttgttgc agaaactatt ggttatgacg
    21421 ttaaatggtt tggaactgaa agaatcatta tgattgattc caaaaatgaa gacagcaggc
    21481 aagaagaaaa tcacgatact acgacggata taatcgatca tgaagatacg gaggatatag
    21541 aagacataag agatattaca gataatgaag ataatggaga caacaaagaa aataaggaca
    21601 atcagacgaa ttctgattca gttatttctg atggaacgga ttttaaacaa aatgttgcaa
    21661 aacttccaac aaaattagct gatgaccctg ttgttattgt atctgataag gaagttccaa
    21721 ctgagtcagc ttcatcagcc atagaaagta aagattatcc gacaaccgat attatttcag
    21781 ctggcgtttt agaaagtgtt agcgatggaa gtcattttaa agtaacggca agttcaccga
    21841 ttagttcatt agaacatatg agtttagaag gcaaaattgt cgtagatatt tataatgcca
    21901 acatgaaact ttcaaatacg aacatggcta ttagcaacaa tccttttgta aaaagtataa
    21961 gaaccagtca gtttagcagt aatccaaaag tgacaagagt tgtatttgat ttaatcaacc
    22021 agaatgatta tgaaattacg atggcttcag acagaatgag ctttattgtt cgaattaaaa
    22081 aatcagtcat tacaaaaata gaacttagtc aaaattcaag aggagatatt ttaacgatat
    22141 tggctaatgc aaaaccttcc gtaacggttt ccagactgac caatccggat cgtttggtaa
    22201 tcgatattcc caatgcggaa gccaatatga aagctattga aaaaacggtt aagggtcagt
    22261 atgtaaaagc catcagaaca tcacaattta atgtggatac ttacagggtt gtattagatc
    22321 ttgaaggtca gcctgaatat gaaattattt ctgtgggagc aaacgggata gctgttcagc
    22381 ttataacacc tacttttaaa aatattaagt acctgaaaga aaatagccag atagctgtgt
    22441 taaagcccaa taaggaattt aatattaatg atattactca caatgatgac tatatgaaca
    22501 ggatatatac cctaacctta cctggagatt attcatctgt ttatggtcaa ggtattttcc
    22561 atattggcga tgaaaagtta aattatcttc agataagcaa tagcctgaat aataaaacag
    22621 aattcaaatt tgctgaaaaa acaatttctg cagttgaaat ctatgataag ggagattatt
    22681 ttgttatttc tcttattaag ccaagagaaa aatacagtaa aattattacc ctggatgctg
    22741 gtcatggcgg ttcagatcct ggagccgtgg gtaatggttt aaaagaaaaa gatgttaatt
    22801 tagatatctt attaaaactc aacgcattat taaaaaatag tcctgatatt aaggtataca
    22861 ctaccaggac cagtgataca tatccaacac ttcaggatag aagtacctta gctaatgaag
    22921 tagaagccga tttcttctta agtattcata ataatgcttt taatgggaaa aatagaggaa
    22981 cggaaacttt atattttcca acagctgcag acaatacgcc gcaattttct ggtaaaaaaa
    23041 tggcacaaat ctttcaaaat aatttagtga gccgtttagg tacaatggac aggggaatta
    23101 aagaacgtcc tggcttatat gttctcagga caacgactat gccagcggtt attgtcgaaa
    23161 ttgcttttat tgacaatgca caagatgccc aattgcttaa atcagactcc tttaagcaag
    23221 aagctgcaag agcattatat gatggaatac tgactatttt tgaacaatat ccaacctatc
    23281 gataaaacca tcctgtacag ggtggttttt tattataaa
//
LOCUS       DAOSUD010000030        23202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1441_length_23202_cov_26.338834, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000030 DAOSUD010000000
VERSION     DAOSUD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 23202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23202
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_23202_cov_26.338834"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..296
                     /locus_tag="PLG49_05465"
     CDS             96..296
                     /locus_tag="PLG49_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOQ16806.1"
                     /translation="MKKGTVKWFNAEKGFGFISVEGEDDVFVHFSAIQGEGFKTLEEG
                     QQVEFEVVEGARGPQAANVSKL"
     gene            480..1970
                     /gene="thrC"
                     /locus_tag="PLG49_05470"
     CDS             480..1970
                     /gene="thrC"
                     /locus_tag="PLG49_05470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOQ16807.1"
                     /translation="MSQIKYKSTRGGQADISSSYAIVQGIAQDGGLFVPQEIPKLDCP
                     LEDLKDMSYQELAYQIMKNYLSDFTEEELKDCINKAYDKKFDDKKIAPLVKHDDVYFL
                     ELFHGPTLAFKDMALSILPHLLKTAAKKLNIEKEIVILTATSGDTGKAALEGFADVEG
                     TKIIVFFPQDGVSEIQKRQMTTQVGDNTYVIGIEGNFDDAQNGVKQIFGDEAFNQYLS
                     ENNYMFSSANSINIGRLVPQIVYYFYAYFQMVQAGEIALGDQVNIVVPTGNFGNILAA
                     YYARQMGLPVAKLICASNDNKVLYDFFNTGKYDKNREFILTISPSMDILISSNLERLL
                     YHIADEDANKTKELMEKLKTQGHYEITPEMKDKLKIFHGGFATQAETCRRIKELFDKT
                     AYLIDTHTAVAYSVYKDYLEKTGDTTKTIIASTASPYKFTTSVMKAIDSQYEGYSDFE
                     LIEKMSQLIKRETPKAIKDIDKRPVRHTTVCKKDEMKAVVEKILIK"
     gene            complement(1980..3233)
                     /locus_tag="PLG49_05475"
     CDS             complement(1980..3233)
                     /locus_tag="PLG49_05475"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ16808.1"
                     /translation="MKKTAKRILWAVMILILLIGSYFYYSKPIEVICLEIVPKTVTKN
                     IHEKGKVVTADEVTLFSPLSAEINKIHIKENQSVKKGDILISYNTREMLYQLEQLKAQ
                     LESIKGQEAQSYQSPSSAQIAQQNLMIEQAKLQKEASEEEFKKAQSLYEDGAISKTAY
                     EEAKRAAETAANLLLQQEQALQILKDQYKAPQGTAQYFKGQKEVIRAQIDLLEYQMSK
                     AEMTAPMDGIISELNVKEKMITAPGTPLLTMIDPKKLEVEVYLLTEDILDIKENMPVE
                     MYLETNKEDVMIKGIVKEIAPYAQQSISALGLEEERIKVSIEINNSPIPLKPGYALQV
                     NFTTFKEDNCISVPKTALFTYQDQDALWIIKNGRAQIQIVEKGLETEQEIIIKNGLNP
                     GDTIIIDPQTKGLKENKKVYPVITG"
     gene            complement(3246..5615)
                     /locus_tag="PLG49_05480"
     CDS             complement(3246..5615)
                     /locus_tag="PLG49_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ16809.1"
                     /translation="MLFRKMLRDLRENKMAYLACIFVISIGIMVFCSMSMVIQDFERA
                     IHNFYTNHNFADGFAYVQEMPLSELKRLENTGGIKKLQGRLIKDVRLYAPGSEKNIYL
                     RMVSVFPDEKNPINGVHLIEGKSLNNNEKNLWLDARFFDTHQLNIGDSIELIIEGKII
                     PFTVMGKGQSPDFAYALRTAQDFYPSPESFGIAYVPYDVMTSLLNQKNTVNEITFTLQ
                     ENASYEDVESRLETKLKTYGLQSIYPRDEQTSHLLLKSELDGLKSVADSIPFLFLLIS
                     SIILYIMIKRLTEQQRGQIGTLKAFGYSNFEILFHYASYALIIGFFGGLIGSLSGLLL
                     TAPMLDIYNEFFNLPDLVPSFQLEYLIYGVLLTMLFSLIAGYEAARGILKLHPAEAMR
                     PPAPPPGKNILIEKIRIFWSSLNTQGRMAARNISRNKGRSFFTFLGIMFTFSLMAFIG
                     SFNRLIDLMIYDQFERIQTADLKISFSGPLSSEEVCRLLSRETGIKKVEPLLEVPARL
                     KNNWHQKDLVILGLEKDSILYNIIDKKGHKIYPPDEGIILSESAAKALDVSVGSYINL
                     ESIWLEDKEDKVTVRVTGIIPQYFGVNAYMNQKKLSQILKEPPMATSALLSLDKKAIK
                     SLKDKYNNSPIISSMEEKEKIIDKYNQMIGSYASMIWILGIFVILTGFAIVYNSSIIS
                     LSERTRELASLRVLGMTQKEVLEVISFEQWSIAFAGMLAGIPMAYAMMETISKSMSTD
                     LFTIPSAIEPPALFTAFWGTTLSVFIAQISTSKKIKQLDLVNVLKELEI"
     gene            complement(5615..6319)
                     /locus_tag="PLG49_05485"
     CDS             complement(5615..6319)
                     /locus_tag="PLG49_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16810.1"
                     /translation="MDTLLKGINISKDYQMGEVTVHALRNVNFEIYDGEFIVVLGPSG
                     SGKSTLLNIIGGMDKPSSGELFFYEQPLHNADEKTLTQYRREAVGFVFQFYNLIPNLT
                     AEENINLSVEIAKNPLKVEDVLKEIDLYDRKDHFPSQLSGGQQQRIAIARALAKNPKI
                     LLCDEPTGALDLSTGIQVLKLLKRFNEIHKKTVIVITHNTEISKIAHRIFYIIDGRIE
                     KIENNPSPVSPEEVNW"
     gene            6511..7020
                     /locus_tag="PLG49_05490"
     CDS             6511..7020
                     /locus_tag="PLG49_05490"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOQ16811.1"
                     /translation="MYKDISFEDYSQEFIKQLPKGVFLNVKGKETINTMTIGWGNIGV
                     IWGKPVMTILVRYSRYTYKLLEESNEFTVSVPLNRQLKKELGFCGTKSGRDYDKFKEC
                     HLTAVKGKEVDAPIIDECDLHFECKVVHKQAMEPALLDENIHENYYSNHDYHVIYYGE
                     IVASYIKQS"
     gene            7036..7545
                     /locus_tag="PLG49_05495"
     CDS             7036..7545
                     /locus_tag="PLG49_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOQ16812.1"
                     /translation="MSLYNEWENHIEQLGTKEQRQKFIQNYLEKEKEAYEKILENKIQ
                     NIEGKVKDLASEYGMTPVEFAGFIDGINTSLTEEVNLEDLTEDSEVKLNIEYEKLYWN
                     MLDAKAEWLYNIPAWADILSEERRKEIKKEYNRSKIVVKPKKIGRNDPCPCGSGKKYK
                     KCCYDKDTR"
     gene            complement(7585..8529)
                     /gene="fba"
                     /locus_tag="PLG49_05500"
     CDS             complement(7585..8529)
                     /gene="fba"
                     /locus_tag="PLG49_05500"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOQ16813.1"
                     /translation="MALVTTKEMFNKAFEGKYAIGAFNINNMEIIQAITRAAGRLKSP
                     VILQVSKSAIKYAGPGYLKKMVDAAIEETGIDLALHLDHGPDLETVKEVIAAGFTSVM
                     FDGSHYDYETNVAKTKEVVEYAHARGVVVEAELGKLAGVEDDVKVSAADATYTDPDQA
                     VDFVRRTGVDSLAVAIGTSHGAYKFKGEAKLDFDRLALITEKLEAAGFHNFPIILHGA
                     SSVDPESVELCNKYGGQIQGAAGVPNEMLRKAASMAVCKINMDTDIRLAMTAAIRQVF
                     GEQPAEFDPRKYLGVARERIEKLVEDKIINVLGSANTL"
     gene            8788..9441
                     /locus_tag="PLG49_05505"
     CDS             8788..9441
                     /locus_tag="PLG49_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOQ16814.1"
                     /translation="MLLFKKEREMLSLVDKHQEAVLECYELFTEGLKELNEKGLNNKV
                     EILAKKVGEAESKADSVRHEIIQSLLKGVLLPESRRELLKLIELTDEIANKCEEIIKQ
                     ICLQQIEFMEEIKPHIREITEKTRIQLIKIRELVNMVFSNMTKAHSLQDELIDIERLE
                     SEVDDEEYNAIYKLFRMKVELARKNQLKSIIADIADISDIAEDISDMLEMIMVLRKV"
     gene            9446..10399
                     /locus_tag="PLG49_05510"
     CDS             9446..10399
                     /locus_tag="PLG49_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOQ16815.1"
                     /translation="MGLPSVLAGAVLGWALGSNGASNCFAAAVSTRIVKYRTAVILTA
                     IFVIIGAYAEGAKGIFKIGDYAYSSGIDTSTKAFFVMLAAALTVTLMTVLHLPVSTSQ
                     AVIGAIIGGALLGGKPDFTEGIKFFIAWFATPFGAIAISFVLYKIFEKYVEKSISNYE
                     IYDRVIIIGYYISGIFAAYSLGGNNVANVTGIYVGKLDILSVKAAVIIGGITIATGVL
                     TYSKGVMKTVGESLVILSPVSGLISVFTSAIVVFIYAKIGIPVSASQAIVGAVIGIGL
                     VKGVNTISFKTIRNIVFAWFGTPTIAGIISLFLFKIFVLNS"
     gene            10492..10821
                     /locus_tag="PLG49_05515"
     CDS             10492..10821
                     /locus_tag="PLG49_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16816.1"
                     /translation="MGKLEIEEIINKIIQMDQKAMESQQKFEKIKKENEKKLKETCRQ
                     MEEDIIQEARRTADNKYKEEIEKCNILVNSILEEGEQKIKRLNDSFEKIRQGLSESIF
                     KEIFEID"
     gene            10834..11871
                     /locus_tag="PLG49_05520"
     CDS             10834..11871
                     /locus_tag="PLG49_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOQ16817.1"
                     /translation="MGSITNFAAINTKIQAKKRYLLNENDYKMLLSKRNVSEIVAYLK
                     NNTGYGELFKNTNPQTIHRGQVEIELYRNIVKQIEDFLPYFQGNYKKFFLALLCEYEI
                     EDLKLILRTINRNEDSSMLEELLIHSKKYSRLDYERLLNSKDMESFFENLKDTVYYIP
                     IKNDNKEDILKREFHIEMQLELILYKTLLERAETLSKEDEKIIKECLGINIDLNNIQW
                     IYRGKKYFHIPPEEILIYCIPGGYRLSYKKLKELVYAPNLKDFIEQIKASKYAFIFPN
                     EEDVFIERRINRYLYKLYLNKQKENRMNIMEAISYIHLLKCEVEDIISVIESIRYNLD
                     KNLLKRYLIRE"
     gene            11895..13895
                     /locus_tag="PLG49_05525"
     CDS             11895..13895
                     /locus_tag="PLG49_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOQ16818.1"
                     /translation="MAVEEMVTMNLVGALKDENKLLRQIILMGNVHLQTDISNIYENY
                     FALNLLEESLARDNHPGEEQTDPNNNNDFNDLFQKAKYIAQALEVDSDAVFSSVDENI
                     DYSQCKSKVCELYHEIYPIYEAILQKQEKIEEYKAYKESIKYISDSEIDFNQLNQLNF
                     FKYKIGLLSHENKMKIIKNIENMTAIIYDIGTRDDLEVFFIIYPQGYETETERILKSL
                     IFKEIIVPEALHGTAAEMKEQIEKVLHQESIELEILFQKINKIKEEYQDIIRQVFVEL
                     KIIMKIESIKEKIIRKNNAFILSGWVPKSQMDEIKNKLSTVSDNFKISFKDVEEIGLD
                     VLPPTKLKNPKLFKPFESLVKMYGIPNYYELDPTAFLSISYLVMFGSMFGDVGQGLIF
                     ILFGLFLQKKNMASFFGQLLTRLGVSSMFFGVLYGSIFGNEELIPWGIKNIFGETSFL
                     SKIVINPMEHINLILLLAIGQGIIFILISFIFGIINALKVHDIKEGLFGRNGVSGLLF
                     YIGLLLLVGDIAFGISVIPKVVIIILMIIAMLLIVIREPLANKILNKSHLYEDEVSAY
                     YVESGFNIIETILSLASNTISFIRVGAFSLNHVGLFLAFKTMAQMAHGGISGFIILII
                     GNIVVIGLEGLIVFIQGLRLQYYELFGKYFKGEGIEFNPVKL"
     gene            13916..14347
                     /locus_tag="PLG49_05530"
     CDS             13916..14347
                     /locus_tag="PLG49_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOQ16819.1"
                     /translation="MRIFLIIAVIIACLTIASGLAGLFKRIKVSKSGLKKSLGLSISS
                     FIVTILGAILTLIPDMAMAAETAAAETASGSGIGFIAAALSTGLATIGAGYAVGAVGS
                     SALGAVSEDPKILGKTLIFVGLAEGIAIYGLIISIMILGRL"
     gene            14349..14654
                     /locus_tag="PLG49_05535"
     CDS             14349..14654
                     /locus_tag="PLG49_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOQ16820.1"
                     /translation="MKSYLISDNKDTLVGLQLAGIKGIVVSEKEEVLREIDKVLQDQE
                     TGILIVTELIMEMAREELMELKMKRRYPLIVEIPDRHGFRKKENLLDYINEAIGVGK"
     gene            14629..15309
                     /locus_tag="PLG49_05540"
     CDS             14629..15309
                     /locus_tag="PLG49_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOQ16821.1"
                     /translation="MKPLALVSEVQKMVTIKEKLNLFSEWLYQDVIEKNEKELKQIEE
                     ENRTYIESYKRELEKRVTSIHRNMENKISQKKNEMISKANLEVKQNILIKKQECFDCL
                     IDEQIKKAKEFTKTKAYEAFFIKTLETVLDKLKNAKSIAISLTKQDRQRFQVLIEELA
                     KKKGLLPANITYQDAPEEIIGGMMVSDEGAAICYNASIMALIEDKKEFIMKKLDEELK
                     KAGDGIDA"
     gene            15302..17080
                     /locus_tag="PLG49_05545"
     CDS             15302..17080
                     /locus_tag="PLG49_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ16822.1"
                     /translation="MHKEGIVTFINGPVVKAENMSLFKVREMVTIGQQRLIGEVISIE
                     NDEATIQVYEETEGLKPGETVKSTGSPLSVKLAPGMIGNIFDGIQRPLKRIDEISPDF
                     IPEGIGLLFLDEEKKWEVHILVEEGQYLKQGQVFAHVPETSVITHKLMVPPGIEGKVI
                     FKKENGHYPIREVLIKLEDKTGTVHELNMIQEWPVRTPRPVSKRRAIEELLVTGQRVL
                     DIFFPIAKGGAAAVPGGFGTGKTVIQHQLAKWSNADIIVYIGCGERGNEMTNVLEEFP
                     RLIDPRTQRPIMERTILIANTSNMPVAAREASIYTGITMAEYYRDMGYDVAIMADSTS
                     RWAEALREISGRLEEMPAEEGYPAYLSSRLAEFYERAGVAESLNGETGSITIIGAVSP
                     PGGDFSEPVTESTKRFVSAFLALDRALAYARHYPAINYLISYSAYVDMLSDWYKENVG
                     EDLLDLRAKLLSILQEEKKLMEIVQLVGEDVLPNDQRLILEIARVIKNGFLQQNALHK
                     TDSFVSLEKQYKMVKTIYHLYERAYKCIKLGIPISQIKDEKLFDEIIKIKYTVENDKI
                     ELIDKVNDDIDLYFDELEEKYKDNLK"
     gene            17092..18465
                     /locus_tag="PLG49_05550"
     CDS             17092..18465
                     /locus_tag="PLG49_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ16823.1"
                     /translation="MRKEILQIDKIDGPLIILSNIDGVSYGEIIDILVDDKTLRKGKV
                     VRIEKDKVVAEVFEGTSGLSTRNVRVRFTGNPLKIPLSKEILGRTFNGIGEPIDFNSP
                     ILSKEKYNINGRPMNPVARVYPRDYINTGISAIDALMTLIRGQKLPIFSGNGLPHNEL
                     AVQIVKQAKIRGSEGGNFAIVFGAMGVKHDDADYFIKSFKESGVLERVVMFLNLADDP
                     IIERISTPRCALTAAEYLAFEHNMHILVILTDMTSYCEALREMSAAREEVPSRKGYPG
                     YMYSDLAGIYERAGQINGKGGSITLLPILTMPNDDITHPIPDLTGFITEGQIVLGREL
                     YQRNIYPPVAVLPSLSRLMKDGIGEKYTREDHADVANQIFSSYSKVQEIRDLAQIIGE
                     EDLSEIDRKYLEFGRKFEEEFLSQRKDENRSIEGTLDLAWKLLSILPIEELDRVDPKI
                     AQKYYRR"
     gene            18470..19078
                     /locus_tag="PLG49_05555"
     CDS             18470..19078
                     /locus_tag="PLG49_05555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ16824.1"
                     /translation="MAVNVAPTKANLIKAKNALALSQKGFELLDKKRNVLIREIMALI
                     DKAKDLHDKMDKAFAKAYQALMVVNITLGIQNVHEIAYSIPRDEEFEILVKSVMGIDI
                     PSIIYEKKELKPSYAFHRTNSALDIAVMEFQEIKYLIYELAEVENSIYKLAKEIEKTT
                     KRANALDNIQIPKYEAQVKYIEEVLEEKEREEFFRLKRLKKG"
     gene            19084..19545
                     /gene="lspA"
                     /locus_tag="PLG49_05560"
     CDS             19084..19545
                     /gene="lspA"
                     /locus_tag="PLG49_05560"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ16825.1"
                     /translation="MLFIVVILVIVLIDQVSKAWAEKKLKLSDWRPTLKNKIYLTLCK
                     NQGAALNLFEKYPKFVNGSSVIAMSLLLFYLLKAIKNNAFFLTKLSLSFIIGGGIGNI
                     IDRSKKGYVVDFFAFKIKKSPIFNIADIFIIFGVILLQLTVLFKKNPIAGE"
     gene            19560..20642
                     /locus_tag="PLG49_05565"
     CDS             19560..20642
                     /locus_tag="PLG49_05565"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOQ16826.1"
                     /translation="MSDRVAQTISTEGLKPGMVLAEDVFNSVGLRLFSEGTRLDDKSI
                     AKIRMYEIDFVLIYEDVNIDYVKAKVEVESKSIRKVHEFKAFKSSYIKNVNTLQDYLT
                     TIGEGKNVSITDLFSLSQEIVSEVQTKSDILAFMHNLKVSDDHTYTHSVNVSLLCNIF
                     GQWLGFDSEKLKNITVAGLIHDIGKIKVDPNILNKPGKLTPEEFEEVKKHPVYGYRMI
                     EKTDMHKDIKLAVLMHHEKYDGSGYPTGAKGNQINDLAKIVAIADIYDAMTSNRSYRD
                     RFCPFKVIQTFEQECYGKLDTKYLLIFLKNIAYNYLDCWVRLSSGEEGQIVFINQNNL
                     SRPIVSIDNVLVDLSMEKDLTIEELI"
     gene            20741..20932
                     /gene="ssrS"
                     /locus_tag="PLG49_05570"
     ncRNA           20741..20932
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLG49_05570"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21006..22922)
                     /locus_tag="PLG49_05575"
     CDS             complement(21006..22922)
                     /locus_tag="PLG49_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16827.1"
                     /translation="MSYDDVLKKIKDDKQKANITSKLYRYTGLIHAIEFFSQKFSIDQ
                     ILEFAYDFVNELILVDQIAIFYKEKNQYTLLKSKGYNIHQYSTEYTPKYDEIALFHGH
                     ILNSHALEEYLPSKVLTDFPSKLAIPLIIESELYGFILTNRFEDKPAFDEEDSIVAEA
                     LMQLFNTSLNNYKSYQSLQTAKMELDEKVFNLFAINQSSKVLLSELDLTVLYNLSIDV
                     FAELTQSSITSFFLYDEKGENYVLRGMRDTFNPSNQLTMSVYLKNDALFDYSKSILDM
                     SIQEDYQLFMDSFQNSEAVIKNLRPLYIVLLIKNKVLLGFVTLGKKVTDKPYNKGIFE
                     LIESLASSTYIAVSNAKYFKETNEQKLLLQKKFNRLVSLNNLMKNINSAATTEELANL
                     TLSTLNISFGIKSGIFALYDNEKFEVIKSLNLENCPASFKLSDKFSCLKEGDYFIINS
                     NEKIPQLLDKEFAESIKNGAGALFVPICIEDAETKLLGLICVFELSNAILSDQESLLT
                     VDTIANHIAPVLYHLLRIKNQAELLTPNHEKIFLNALEKHIQEAEDLFLDLTLIHIHN
                     PDFSFEAIDVPKDIKDNYKDIYFLTKSDVFVVAFEKADEKTIRDLVDEKYLLSFYRYK
                     KDFSSLEEFMNLFK"
     gene            complement(22953..>23202)
                     /locus_tag="PLG49_05580"
     CDS             complement(22953..>23202)
                     /locus_tag="PLG49_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16828.1"
                     /translation="HQYEFKRILKDISNLREELKRLQAERKNYLSYLRAKGEGEITIY
                     GIVHGNTTFEIKNIQKRFMDQTRGPITYYVLENELKSS"
BASE COUNT         7895 a         3465 c         4464 g         7378 t
ORIGIN      
        1 atatttttca taaacaccgt tgacagcggt aaaaatgtat ggtaacttag acataggctt
       61 tatgccaaat atattaatgt tttggaggat tttaaatgaa aaaaggcact gtaaaatggt
      121 ttaacgcaga aaaaggtttt ggatttattt ctgtggaagg agaagatgac gttttcgtac
      181 atttctctgc tatccaaggc gaaggtttta aaactttgga agaaggtcaa caagtagaat
      241 tcgaagtagt cgaaggcgct agaggacctc aagcagcgaa tgtatctaaa ttataataat
      301 tcatattatt atatatttaa atatatcgct acacaatcat accacccctt agagggtggt
      361 atttttgttg aaaaagagtt aaaaatttat caaatttatg ttataattta tgaaactttt
      421 ttatttttat tgtgttataa ttgaaaataa attttttatc agtgttagga ggaaaaccaa
      481 tgagccagat taagtataaa agtaccagag gaggacaagc agatatttct tcttcatatg
      541 cgattgtcca ggggattgca caagatggcg gtttatttgt acctcaggaa attcctaagc
      601 tcgattgccc tttagaagat ttaaaagata tgagctatca ggaattggcc tatcaaatta
      661 tgaaaaatta cttatctgat tttacagaag aagagctgaa agattgtatt aataaagctt
      721 atgataaaaa atttgatgat aaaaagattg cgcctttagt aaaacatgac gatgtttact
      781 ttctggagct tttccatggg ccaactttgg cttttaaaga tatggccctt tcaattttac
      841 ctcatttatt aaagactgct gcaaaaaaat taaacattga aaaggaaatt gttattttga
      901 cagcaacttc aggggataca ggaaaggcag ccctggaagg ctttgctgat gtagaaggta
      961 caaagattat tgtctttttc ccgcaagatg gtgtgagcga aattcaaaaa agacaaatga
     1021 ctacccaggt gggagataac acctatgtta tagggataga aggtaatttt gatgatgccc
     1081 agaatggtgt aaagcaaatt tttggagatg aagcttttaa tcagtattta agtgaaaaca
     1141 attatatgtt ttcttctgcc aattcgatta atatcggccg tttggtaccc cagattgtat
     1201 attattttta tgcttatttc caaatggtcc aggctgggga aattgcttta ggagaccagg
     1261 tcaatattgt tgtaccaaca ggtaactttg gtaatatttt ggctgcttat tatgcaaggc
     1321 aaatgggact tcctgttgcc aaattaatct gtgcttccaa tgacaataaa gttttatatg
     1381 atttcttcaa cacagggaaa tatgataaga acagagaatt tattttaacg atttctcctt
     1441 ctatggatat tttaatatcc agtaacttag aaagattact ttatcatatt gccgatgaag
     1501 atgccaataa gaccaaagaa ttgatggaaa agttaaaaac acaaggtcat tatgaaatta
     1561 cacctgaaat gaaagataaa ttaaaaattt tccatggcgg ttttgcaacg caggcggaaa
     1621 cctgtagaag aattaaagag ctgtttgata agactgcata tcttatagat actcataccg
     1681 ctgtggctta ttcggtttat aaagattatc ttgaaaaaac aggagacact acaaaaacca
     1741 tcattgcatc cacggcaagt ccttataaat ttacaaccag tgttatgaaa gctatcgata
     1801 gccagtacga aggttattca gattttgaat taattgaaaa aatgagccag ttaataaaga
     1861 gggaaacccc aaaagcgata aaagacattg ataaaaggcc tgttcgtcat acgacagttt
     1921 gcaaaaagga cgaaatgaaa gcagtcgtag aaaaaatatt aataaaataa taaaaaagtt
     1981 tatccagtga taactggata aacttttttg ttttctttaa gacctttggt ctgggggtcg
     2041 atgataatgg tgtctccggg attaagccca tttttaataa tgatttcctg ctctgtttct
     2101 aatccttttt ctacgatctg gatttgagcc cggccatttt taataatcca gagagcatcc
     2161 tggtcctgat aagtaaagag ggctgttttt ggaacactaa tacaattgtc ttctttaaaa
     2221 gttgtaaagt taacttgaag ggcataaccg ggttttaaag gaatgggtga attatttatt
     2281 tcaatgctta ctttaattct ttcttcttcc agtcctaaag ctgaaatgct ttgttgggca
     2341 taaggagcga tttcttttac aattcctttt atcattacat cttctttatt agtttccaaa
     2401 tacatttcta caggcatatt ttctttaata tcgaggatat cttctgttaa aagataaact
     2461 tcaacttcaa gtttttttgg gtcaatcatt gtaagcagtg gagtacctgg ggcagtaatc
     2521 attttttctt ttacatttaa ttcactgatg attccgtcca taggagcagt catttcagct
     2581 ttgctcattt ggtattcgag taaatcaatt tgtgcacgga tgacttcttt ttgtccttta
     2641 aaatattgag cggtaccttg tggggcttta tattgatcct ttaagatctg aagggcttgt
     2701 tcttgttgca gtaaaagatt tgctgccgtt tcggcggctc tttttgcttc ttcataagca
     2761 gttttactaa tggctccgtc ttcataaagc gattgagctt ttttaaattc ttcttcagag
     2821 gcttcttttt gaagttttgc ctgttctatc attaaatttt gctgtgcaat ttgagctgaa
     2881 gaaggacttt gataactttg ggcttcctga cctttgatac tttccagttg agcttttagc
     2941 tgttccagtt gatagagcat ttctcgagta ttatagctta taaggatatc accttttttt
     3001 acagactgat tttcttttat atgaatttta ttaatttcag cagacaatgg gctaaaaagg
     3061 gttacttcat cggcagttac aacttttcct ttttcatgaa tattttttgt tacggttttt
     3121 ggcactattt ccaaacaaat gacttcaata ggcttgctat agtaaaaata gctgccaata
     3181 agaagaatta aaatcattac agcccaaaga attcttttag ccgtcttttt catttccagc
     3241 acctcctaga tttccagttc ctttaggaca tttactaagt ctaactgttt tatttttttg
     3301 ctggttgaaa tttgtgcaat aaaaactgaa agcgtggtgc cccaaaaagc agtaaaaaga
     3361 gcagggggct caatagctga cgggatagta aacaaatccg tggacatact tttagagatg
     3421 gtttccatca tggcataagc catcggtatt cctgcaagca tgcctgcaaa agcaatgctc
     3481 cactgttcaa agctaatcac ttctaaaact tccttttgcg tcatacccag aaccctcaaa
     3541 gatgccaatt ctcttgtcct ttcggataag gaaataatac tggaattata aacgatagca
     3601 aagcctgtga gaatcacaaa aattcccaat atccatatca tggatgcata ggagccaatc
     3661 atttgattgt atttatcaat tattttttcc ttttcttcca tcgaagaaat aataggagaa
     3721 ttattatatt tatcctttaa ggattttata gcttttttat ctaaggagag taaagccgaa
     3781 gtggccatag gcggttcctt caatatttga cttaactttt tttgattcat ataagcgttt
     3841 acaccaaaat attgggggat aataccagtt accctgacag taaccttatc ttctttatct
     3901 tcaagccaga tactttcaag gtttatataa ctgcctactg agacatcaag ggcttttgct
     3961 gctgactcag aaaggataat cccttcatct ggaggataaa ttttatgccc tttcttgtca
     4021 atgatattat aaagaataga atctttttca agtcctaata tgaccaaatc tttttgatgc
     4081 cagttatttt ttagacgggc tggaacttcc aataaaggtt cgaccttttt aataccagtt
     4141 tcacggctta aaagcctgca aacttcctcg gaagaaaggg gaccagaaaa agatattttc
     4201 aaatcagcgg tttggattct ttcaaactga tcgtatatca tcaaatcgat caagcgattg
     4261 aaagagccaa tgaaagccat caaactaaag gtaaacatga ttcctaagaa ggtgaaaaag
     4321 gatctgcctt tatttctcga tatatttctt gctgccatgc ggccctgggt attaagggaa
     4381 gaccaaaaaa ttctaatttt ttctatcaag atatttttac ccgggggagg agcaggaggc
     4441 cgcatggctt ctgcaggatg gagtttaagg attcccctgg cagcttcgta accagcaatt
     4501 aggctaaaaa gcatggtaag aagaacacca tagataaggt attccagctg aaaacttggg
     4561 accaaatcag gaagattaaa aaattcatta tagatatcca acataggagc ggttaataaa
     4621 agacctgata aagaaccaat cagtccgcca aaaaaaccaa taataagggc ataagaagca
     4681 taatgaaaga gaatttcaaa gttggaataa ccaaaggctt tcagggttcc tatttgtcct
     4741 ctttgctgtt ctgtcagccg tttaatcata atatataaga tgatgctgga gattaataaa
     4801 aataaaaaag gaatagaatc ggcaacactt tttagaccat ctaattcaga ttttaagagc
     4861 aggtggctgg tttgttcatc cctgggataa atgctttgga gcccatacgt ttttaatttt
     4921 gtttctaagc ggctttcgac atcttcatag cttgcgtttt cctgtaaggt aaaagtaatt
     4981 tcattaaccg tatttttctg attcaacaag gaagtcatga catcataagg aacataggca
     5041 atcccaaagc tttcaggaga aggataaaaa tcttgggcag ttcttaaggc gtaagcaaaa
     5101 tcagggcttt gcccttttcc catgactgta aagggaatta ttttaccttc tataatgagt
     5161 tctatggaat ccccgatatt aagctgatgg gtatcaaaaa agcgggcatc taaccataga
     5221 tttttttcgt tattattaag agatttgcct tctatcagat gtacaccgtt tataggattt
     5281 ttttcatcag gaaaaacaga aaccattctc agatagatgt ttttttcact gccaggggca
     5341 taaagcctaa catctttaat cagccttcct tgcagctttt taattccccc ggtattttca
     5401 agccttttca gctccgataa aggcatttct tgaacatagg caaaaccatc tgcaaagttg
     5461 tgattggtgt agaaattatg aatagccctt tcaaaatcct gaatgaccat agacattgaa
     5521 caaaagacca taataccgat ggaaatgaca aagatacagg ccaggtaagc catcttattt
     5581 tctcttaaat ctctgagcat tttcctgaaa agcattacca gttcacctcc tccggtgaaa
     5641 caggtgatgg attgttttca attttctcaa ttcgtccatc aataatataa aagattctat
     5701 gggcaatttt agaaatttct gtattatggg tgatgacaat tacagttttc ttatggattt
     5761 cattgaatct tttaagaagc tttaagactt gtattcctgt tgataagtcc aatgcgcctg
     5821 tgggctcatc gcaaagtaaa atttttggat ttttggcaag agctctggca atagcaattc
     5881 tttgctgctg gccgccagaa agttgagaag ggaagtggtc ttttctgtca tataaatcaa
     5941 tttctttaag gacatcttca acttttaaag gattttttgc aatttctaca gaaagattaa
     6001 tgttttcttc tgccgtcagg tttggaatca gattataaaa ttgaaagaca aagccaacgg
     6061 cctctctcct gtattgagtt aaagtttttt catcggcatt gtgcaggggt tgttcataaa
     6121 aaaataattc cccagagcta ggtttgtcca ttcctcctat gatatttaag agagtacttt
     6181 ttcctgaacc actgggacct aaaacaacaa taaattcccc atcatagatt tcaaaattta
     6241 catttctaag agcatgaacc gttacttcac ccatttgata gtctttagaa atattaattc
     6301 cctttaataa tgtgtccatg atccacctcc gtggataaag agtgcgtact aaccaaacat
     6361 agagtctaac tctacctaac acaatcgtag cactgattta aaaaaaggtc aatagaaatt
     6421 ggtattttac acataatata tgagtatgat atattattat attagttggg caatttaatt
     6481 cattttaaga ttaaataaag gaggttattt atgtataaag atatttcttt tgaagattat
     6541 agccaggagt ttattaagca attgccaaaa ggtgtttttt taaatgtaaa aggcaaagag
     6601 accataaata ccatgaccat tggctggggc aatattgggg tgatttgggg caagcctgtc
     6661 atgaccatat tggttagata ctccagatat acatacaagc ttttagaaga atctaatgaa
     6721 tttactgtta gtgttccctt aaacaggcaa ttaaaaaaag aattgggttt ttgtgggaca
     6781 aaatctggaa gggactatga caaatttaaa gaatgtcatt tgactgcggt aaaaggaaaa
     6841 gaagttgatg caccgattat tgatgaatgc gatttacact tcgaatgcaa agtcgtacat
     6901 aaacaagcaa tggagccagc actattagat gaaaatattc atgaaaatta ctattccaat
     6961 catgattatc atgtgatata ctatggtgag attgtagcaa gttatataaa acaaagttag
     7021 gaaagaggta gttttatgag tctttacaat gagtgggaaa atcatattga acaattagga
     7081 acaaaagaac aaagacaaaa atttatccaa aactatcttg aaaaagaaaa agaagcttac
     7141 gaaaaaatcc tggaaaataa aatacaaaat attgaaggaa aagttaagga tctggcaagt
     7201 gaatatggta tgacaccagt tgaatttgct ggttttatcg atggaattaa taccagcttg
     7261 acagaagaag tgaatttaga agacctgaca gaggattcag aagtaaaatt aaacattgaa
     7321 tacgaaaagc tttattggaa tatgctggat gccaaagcgg aatggcttta taacattcct
     7381 gcctgggctg atattttaag tgaagaaaga agaaaagaaa ttaaaaagga atacaatcgt
     7441 tcaaagatag ttgtaaaacc taagaaaatt ggaagaaatg atccctgtcc ttgcggcagt
     7501 ggtaaaaaat ataaaaagtg ctgttatgat aaagacaccc gttaaagcgg gtgtcttata
     7561 tattttaggc tttattaaaa tgaattatag agtattagca gaacctaaaa cgtttatgat
     7621 tttgtcttct actaattttt caattctttc tctggctaca ccaagatatt ttcttgggtc
     7681 aaattcagcc ggttgttccc cgaatacttg tctgattgct gcagtcatag ccagtctgat
     7741 gtctgtgtcc atattaattt tacatacagc catggaagca gcttttctta gcatttcatt
     7801 aggtacaccg gcagctcctt gaatttgtcc gccgtatttg ttacaaagtt caacagattc
     7861 agggtcaaca gaagaagcac cgtgaagaat aatggggaag ttatggaagc cagctgcttc
     7921 taatttttca gtaattaaag ctaaacggtc aaaatctaat tttgcttctc ctttaaattt
     7981 atatgcacca tggcttgtac caatagccac tgccaaagag tcaacaccag ttcttcttac
     8041 aaagtcaact gcctggtcag ggtcggtata tgtagcatct gcagctgaaa ctttaacatc
     8101 gtcttctacg cctgccagtt ttcctaattc agcttctaca acaacacctc tggcatgagc
     8161 atattcaacc acttcttttg tttttgctac attggtttca tagtcataat gagaaccgtc
     8221 aaacataaca gaagtaaaac cagctgcaat gacttcttta acggtttcca aatcaggacc
     8281 atggtctaaa tgaagggcta aatcaatgcc ggtttcttca atagccgcat caaccatttt
     8341 ctttaaatat ccaggtccgg catatttaat agcacttttg gatacctgaa gaataactgg
     8401 agattttaat ctgccagcgg cgcgggtaat cgcctgaatg atttccatat tattgatgtt
     8461 gaaagcacca atagcatatt tcccttcaaa tgctttgtta aacatttctt ttgttgttac
     8521 taatgccatt tttattccac ctttcaaaat gattaattat ttttggaagt cacttgtctt
     8581 ttattccttc cttattataa tagttcacaa aatatattac aacattttta attttaaata
     8641 aaagtaaaac tagatacttt tttggtatat tataacatat aataaataaa tttacaatga
     8701 ttatcactta aaatgaaatg gttactttca taattttttg tgatatagta aaaatataaa
     8761 ttagtcataa aaaagaaggt gaaggtaatg ttacttttta aaaaggaaag agaaatgcta
     8821 agtctggtcg ataagcatca ggaggctgtg ctggaatgtt atgaattatt tactgaaggt
     8881 cttaaagaat taaatgaaaa gggattaaat aataaagttg aaatattggc aaaaaaggta
     8941 ggagaagcag aatctaaagc agattctgtc cggcacgaaa tcattcagtc gcttcttaaa
     9001 ggcgttttac ttcctgaatc aagacgagag ttattaaaat taattgaatt aaccgatgaa
     9061 attgcaaata aatgcgaaga aatcattaaa caaatttgcc tgcagcaaat agagtttatg
     9121 gaagaaataa aaccgcatat cagggaaatt acggaaaaaa ccagaattca actgataaaa
     9181 ataagagaat tagtcaatat ggtatttagc aatatgacaa aagcccatag ccttcaagat
     9241 gaacttatcg atattgaaag gctggagtca gaagtcgacg atgaagaata taatgcaata
     9301 tataaactgt ttagaatgaa agtagaactg gcaagaaaaa accaattaaa atctatcata
     9361 gcagatatag cagatatttc tgatatagca gaggatatat ctgacatgtt agaaatgatt
     9421 atggtactta gaaaggtttg ataatatggg tttgccgtca gtattagccg gggcagtttt
     9481 aggttgggcc ctgggcagca acggggcttc caattgcttt gctgctgcag tatccacaag
     9541 aattgttaaa tacagaacag ctgtaatttt aactgcaatt tttgtaatta ttggcgctta
     9601 tgcagaagga gcaaaaggga tctttaaaat aggagattat gcttatagca gcggaattga
     9661 tacatcaacc aaagcttttt ttgtaatgct tgctgcggca ttaacggtga ctttaatgac
     9721 ggtactgcat ttacctgtat caacatctca ggctgttata ggtgctatta ttggtggagc
     9781 cctgcttgga ggcaagcctg attttacgga gggcattaaa ttttttattg catggtttgc
     9841 aactcctttt ggtgccatcg ccatttcatt tgttttgtat aaaatttttg aaaagtatgt
     9901 cgaaaaaagt attagtaatt atgaaattta tgatagagtt atcatcattg gttattatat
     9961 ttctggtatt tttgcagctt attctttagg tggcaataat gttgccaatg tgacaggtat
    10021 ttatgtggga aaattagaca tattaagtgt aaaagctgct gttataatag gcgggattac
    10081 catagctact ggtgttctta cctatagcaa gggcgttatg aaaacggtag gggaaagcct
    10141 ggtgattctt tctcctgttt ctggcttaat atcggttttt acttcagcaa ttgttgtatt
    10201 tatttatgca aaaataggta ttcccgtatc tgcatctcag gcaattgttg gtgctgtaat
    10261 tggaataggt cttgtaaaag gggttaatac cattagtttt aaaaccattc gcaatattgt
    10321 ttttgcctgg tttggaaccc ctacaattgc tggaataatc agtctttttt tattcaaaat
    10381 ttttgtattg aattcataaa aaaatgggag tataatgtag ttgatgattg ggaagtacca
    10441 ttatatggaa attattatct aatctataaa aagctataag gagtaaggga aatgggcaag
    10501 cttgagatag aagaaattat aaataaaatc atccaaatgg atcaaaaagc tatggaaagt
    10561 caacaaaagt tcgaaaaaat aaaaaaggaa aatgaaaaaa agcttaaaga gacctgcagg
    10621 caaatggaag aagacattat tcaggaagcc agaagaactg cagataataa atataaagag
    10681 gaaatcgaaa agtgtaatat acttgtaaat tccattttag aagaagggga acaaaagata
    10741 aaaagattaa atgattcttt tgagaaaatt cgtcagggac tttctgaatc gatctttaaa
    10801 gaaatatttg aaattgatta ggagatttta caaatgggaa gcataacgaa ctttgcggca
    10861 attaacacta aaatacaggc aaagaaaaga tatttattaa atgaaaacga ttataaaatg
    10921 cttctatcga agagaaatgt ttcggagatt gtggcctatc ttaaaaataa tacaggttat
    10981 ggtgaattat ttaaaaatac gaatcctcag acgattcaca gagggcaagt tgagatagag
    11041 ttgtacagaa atattgttaa gcagatagaa gattttctgc cttattttca aggaaattat
    11101 aagaagtttt ttttagccct tttatgtgaa tatgaaattg aagatttaaa gctaatcctg
    11161 agaacgatta acagaaatga agacagttct atgcttgaag agttactgat tcattcaaaa
    11221 aaatatagcc gtttggatta cgaaaggcta ttaaattcaa aagacatgga aagtttcttt
    11281 gaaaacctta aagatacggt ttattacatt cctataaaaa atgataataa agaagacata
    11341 ttaaaaagag aattccacat tgaaatgcag ttggagctta ttttatacaa aactttactg
    11401 gaaagggcag aaactttatc caaagaagat gaaaagatta ttaaagaatg tttagggatt
    11461 aatattgatt taaataatat ccagtggatt tacaggggta agaaatattt tcatattccc
    11521 ccggaagaaa tattaatcta ttgtatccct ggtggttatc ggttaagtta taaaaaatta
    11581 aaagagcttg tttatgcgcc aaacctgaaa gattttattg aacaaatcaa ggcttcaaag
    11641 tatgctttca tttttcccaa tgaagaagat gtatttatag aaagaagaat taacagatat
    11701 ctttataaat tatatttaaa caagcaaaaa gaaaaccgga tgaacatcat ggaggcaatt
    11761 tcatatattc atttattaaa atgtgaagtt gaagatatca ttagtgtgat agaatccatc
    11821 cgttataatt tagataaaaa tctcttaaaa aggtatttaa tacgagaatg ataaaagcga
    11881 tggaggtctt ttttatggca gtcgaagaaa tggtaaccat gaatttagtg ggtgccttaa
    11941 aagatgagaa caaacttcta aggcaaatta ttttgatggg caatgttcat ttgcaaaccg
    12001 acataagcaa catttatgaa aattattttg cccttaatct tttagaagaa agtcttgcca
    12061 gggacaatca tcctggtgaa gaacaaactg atccaaataa taacaacgat tttaatgatt
    12121 tgttccaaaa ggcaaaatat attgcacaag cgttagaagt agattctgac gcggtttttt
    12181 cttctgttga tgaaaacatt gactatagtc aatgtaaaag taaagtttgt gagctttatc
    12241 atgaaattta tcctatttac gaagcaattt tacaaaaaca agaaaaaatt gaagagtata
    12301 aggcttataa ggaaagtatt aaatatatat ctgattcaga gattgatttt aatcagttga
    12361 atcagttgaa tttttttaag tataaaatcg gcttgctgtc ccatgaaaat aaaatgaaaa
    12421 ttatcaaaaa tatcgagaat atgacagcta ttatttacga tattggaaca agggatgatc
    12481 tggaagtatt ctttattatt tatcctcagg gatatgaaac tgaaaccgaa agaattttaa
    12541 aatctcttat tttcaaagaa ataatagttc cggaagcatt acatggaacg gcggcagaaa
    12601 tgaaagagca gatagaaaaa gtgcttcatc aggaaagtat tgaattagaa atactttttc
    12661 agaaaataaa caagataaaa gaagagtatc aggatattat taggcaagtg tttgttgaat
    12721 taaagattat catgaagatt gaaagcatca aagaaaaaat tataagaaag aataatgcct
    12781 ttattttatc tggttgggtt cccaaaagcc aaatggatga aataaaaaat aaactttcaa
    12841 cagtcagtga taattttaaa ataagtttta aagatgttga agagattggc ttagatgtct
    12901 tgccacccac taaattaaaa aatccaaagc tgtttaagcc tttcgaaagt ttagtaaaga
    12961 tgtatggcat tccaaattat tatgaattag atccgaccgc atttttgagt atatcttatc
    13021 ttgttatgtt tggttctatg tttggagatg tgggtcaggg gctgattttt atactgtttg
    13081 gacttttcct tcaaaaaaag aatatggctt ctttttttgg tcaactttta acccgtttag
    13141 gggttagttc gatgtttttt ggcgttttgt atggcagtat ctttggaaat gaagaattaa
    13201 tcccatgggg aattaagaat atatttggag agacaagctt tctttcaaag attgtcatca
    13261 atcctatgga acacatcaat ctaatcttac ttctggccat tggacagggt atcattttta
    13321 tattaatcag ctttattttt ggcattatca atgccctgaa agtacatgat ataaaagaag
    13381 gtctctttgg aagaaacggg gtatccggac tgctctttta tattggttta ttacttttag
    13441 tgggcgatat tgcgtttggt atttctgtta ttcctaaagt ggttattatt atcttaatga
    13501 ttatcgcgat gctgctgata gtcatcaggg aacctctggc caataaaatt ttaaataagt
    13561 ctcatctgta tgaggatgaa gtatctgctt actatgttga aagtggattt aatatcatag
    13621 aaaccatttt aagcttagcc agcaatacca tttcttttat cagggttgga gccttttctt
    13681 taaaccatgt gggcttgttt ttagcgttta aaaccatggc acagatggcc catgggggta
    13741 tctcaggctt tattatcctg ataataggga atattgttgt aataggctta gaaggactga
    13801 ttgtcttcat tcaaggtttg cgccttcaat attatgaact gtttggtaaa tattttaagg
    13861 gtgaaggaat agaatttaac ccggttaagc tgtaataaat aagaggagga atattatgag
    13921 gatattttta atcattgctg ttatcattgc atgtttaaca attgcttcag gtttggcagg
    13981 tttatttaag agaattaaag tttctaaatc aggtcttaag aaaagtttag gtcttagcat
    14041 aagctctttt attgtaacca tccttggagc cattttgacg cttattcccg atatggctat
    14101 ggcggcagaa acagctgctg cagaaacagc gtcaggctca ggcataggat ttattgcagc
    14161 agcccttagt acaggtcttg caaccattgg tgctggttat gcagtaggtg cggttggatc
    14221 ttctgcttta ggtgcagttt ctgaagatcc taaaatcttg ggtaaaacgc ttatttttgt
    14281 tggattggca gaaggtattg caatttatgg tcttattata tccattatga ttttaggaag
    14341 gttataatat gaagtcatat ttaattagtg ataataaaga tactttagtt ggcctgcagt
    14401 tggcaggcat taagggtatt gttgtcagtg aaaaagaaga ggttttaaga gaaattgata
    14461 aggttcttca agatcaggaa acaggtatct tgattgtaac cgaattaatc atggaaatgg
    14521 ccagagaaga gttaatggaa ttaaagatga aaagaagata ccctcttatt gttgaaatac
    14581 ctgaccgtca tggcttcaga aagaaagaaa acttactgga ttatattaat gaagccattg
    14641 gcgttggtaa gtgaggtgca aaaaatggta accataaaag aaaaattaaa tcttttttca
    14701 gaatggcttt atcaagatgt tattgaaaag aatgaaaaag aattaaaaca aatagaagaa
    14761 gaaaacagga cttatataga aagctataaa agagaattag aaaaaagagt cacaagtatc
    14821 catcggaata tggaaaataa aatcagtcaa aagaaaaatg aaatgatcag taaagcaaat
    14881 ttagaagtta agcaaaacat acttataaaa aagcaggaat gctttgactg tttaattgat
    14941 gaacagatta aaaaagcaaa agaatttaca aagacgaagg cgtatgaagc attttttata
    15001 aagaccctgg aaactgtact ggataagtta aaaaatgcaa aaagcatagc gattagctta
    15061 acaaagcagg accggcaaag atttcaggtt cttattgaag agctggcaaa gaaaaaagga
    15121 cttttgccag ccaatataac ttatcaggat gcccctgagg agattatcgg agggatgatg
    15181 gtatccgatg aaggagcagc catatgttat aatgcaagta ttatggcatt aatagaagat
    15241 aaaaaagagt ttattatgaa gaaattagat gaagaattaa aaaaagctgg tgatggtatt
    15301 gatgcataaa gaaggaattg taacctttat taatggccca gttgtaaagg cagaaaacat
    15361 gtctcttttt aaggttaggg aaatggtgac tataggtcag caacgcctta ttggtgaagt
    15421 gatttctata gaaaatgatg aggccaccat acaagtttat gaagagacag aaggcttaaa
    15481 gcctggtgaa accgtaaagt ctactggcag cccgctttct gtaaaactgg cccctggcat
    15541 gattgggaat atttttgatg ggattcaaag acctttaaaa agaatcgatg aaatttctcc
    15601 tgattttatt cccgaaggca ttgggcttct ttttttagat gaagaaaaga agtgggaagt
    15661 tcatatctta gtcgaagaag ggcaatattt aaaacaaggg caagtttttg cccatgttcc
    15721 tgaaacgtct gttattaccc ataaactcat ggttcctcca gggattgaag ggaaagtcat
    15781 ttttaaaaaa gaaaatggac actatcccat aagggaagta ttgataaaac ttgaagacaa
    15841 gacgggaaca gttcatgagc tcaatatgat tcaagaatgg cctgtcagaa ctcccagacc
    15901 agtatccaaa agaagagcca ttgaagaatt attagtcact ggtcaaagag ttcttgatat
    15961 ttttttcccc attgcaaaag ggggagcagc agcggttcca ggcggctttg gtacagggaa
    16021 aaccgttatc cagcaccagc ttgctaaatg gtccaatgca gatattattg tttatatagg
    16081 ctgcggagaa cgtggaaatg aaatgaccaa tgttctggaa gaattcccaa ggctaattga
    16141 tccaagaact caaagaccga ttatggaaag aaccatatta attgccaata cttccaatat
    16201 gcctgttgca gccagggaag caagtattta tactggcata accatggcag agtactatag
    16261 ggatatgggt tatgatgtgg ccattatggc agattcaact tccagatggg cggaagctct
    16321 tagagaaatt tcaggaagac ttgaagaaat gccggcagaa gaaggttacc cggcatatct
    16381 gtcttccaga ttagccgagt tttatgaaag agctggtgtt gcagaatctc ttaatggtga
    16441 gacaggttcg attaccatta ttggagcagt atcccctcct ggaggggact tttccgaacc
    16501 cgttacggaa agcaccaaac gctttgtcag tgctttttta gctctggaca gagctttggc
    16561 ctatgcaaga cactatccgg ccattaatta tttaatcagc tatagtgctt atgtcgatat
    16621 gctttctgac tggtataaag aaaatgtcgg ggaagatctt ttagacttac gggcgaaact
    16681 gcttagcatt ttgcaggaag aaaagaaact gatggaaatt gttcaattgg taggagaaga
    16741 tgtcttgcct aatgaccaaa ggcttatttt agaaattgcc agagtcataa aaaatggctt
    16801 tttacaacaa aatgccttgc ataagacaga ctcttttgtc agcttagaaa agcaatataa
    16861 aatggtaaaa acaatctacc atttatacga aagggcatat aaatgtataa aacttggtat
    16921 tcctatatca cagataaaag atgaaaaact ttttgatgaa attataaaga ttaaatatac
    16981 cgttgaaaat gacaaaattg aattgattga taaagtgaat gatgacattg atttatattt
    17041 tgatgaacta gaagaaaagt ataaggataa cttaaaatag gatgtgatgt cgtgaggaaa
    17101 gaaattcttc agatagataa aatagatggt cctctaatta tactttcaaa tatagatggc
    17161 gtttcttatg gagaaatcat agacattctg gtggatgata agactttaag aaaaggcaaa
    17221 gttgtaagaa tcgaaaaaga taaggtggtt gccgaagttt ttgaaggaac ttcaggcctt
    17281 tccacaagaa atgtcagggt tagatttaca ggtaatcctc ttaaaatccc tttgtccaaa
    17341 gaaattttag gcaggacctt taacggtatt ggagagccta ttgattttaa ttcgccaatt
    17401 ctttcaaaag aaaaatacaa tattaatgga aggccaatga atcctgtagc cagggtttat
    17461 ccaagggact atattaatac aggaatttca gcgattgatg cgcttatgac cttaataaga
    17521 ggacaaaaat tgccgatatt ttcagggaat gggcttcctc ataatgaatt agccgttcag
    17581 attgttaaac aagccaaaat acgaggaagt gaaggcggaa attttgccat tgtctttggg
    17641 gcaatgggtg taaagcacga tgatgctgat tattttataa agagctttaa agaatccggg
    17701 gtattagaaa gagtggttat gtttttaaac ctggctgatg accccatcat tgaaaggatt
    17761 tctaccccca gatgtgccct gacggcagca gaatatcttg cctttgaaca taacatgcat
    17821 attcttgtta tattaacgga catgaccagt tattgtgagg ctttaagaga aatgtctgca
    17881 gccagggaag aagtgcctag cagaaaaggt tatcccggat atatgtatag tgatttagca
    17941 ggtatttatg aaagagcagg tcaaattaat gggaaaggtg gatccattac ccttttacct
    18001 atattgacga tgcccaatga cgatattacc catccgattc cagacttaac aggttttatt
    18061 acagaaggac aaattgtgct gggaagagaa ttatatcaga gaaatattta tccgccagtt
    18121 gctgttttac cttcactttc aagattgatg aaagatggta ttggggaaaa gtatacaaga
    18181 gaagatcatg ccgatgtagc caatcagatt ttttcctctt attctaaagt acaagaaata
    18241 agagatttgg cccagattat tggtgaagaa gatttgtcgg aaattgatag aaagtatttg
    18301 gaatttggcc ggaaatttga agaggaattc ctaagccaga gaaaggatga aaaccgttct
    18361 atagagggta cccttgattt ggcctggaaa ttgctttcca tcctcccaat agaagagtta
    18421 gacagagttg atccgaaaat agcccaaaaa tattacagaa ggtgatttga tggctgtaaa
    18481 tgtagcaccg actaaggcca atctgattaa agcgaaaaat gccctggcgc tttcccaaaa
    18541 gggctttgaa cttttagata aaaaaagaaa tgtattaata cgggaaatca tggctcttat
    18601 tgataaggct aaagatttac acgataaaat ggataaggca tttgcaaaag cttatcaagc
    18661 cttaatggta gtcaacatta ccttaggtat ccaaaacgtt catgaaattg cttactcgat
    18721 tcccagggat gaagaatttg aaattttagt aaaaagcgtc atgggtattg atattccttc
    18781 tattatttat gaaaaaaagg aactcaaacc gagctatgcc tttcacagga ccaattctgc
    18841 tctggatatt gctgtgatgg agtttcagga aataaaatat ttaatttatg agttggcgga
    18901 agtggaaaat tctatatata aattggcaaa agaaattgaa aaaacaacaa aacgtgccaa
    18961 tgccttagat aacattcaaa taccaaaata tgaagcacaa gttaaatata ttgaagaagt
    19021 attagaagaa aaagaaaggg aagaattctt tagattaaaa aggttgaaga aaggttagat
    19081 aggatgttat ttattgttgt gatacttgtt attgttctta tagatcaagt cagcaaagcc
    19141 tgggcagaga aaaagttaaa gttaagtgac tggaggccga cattaaaaaa taagatttat
    19201 ttaacccttt gcaagaacca gggggctgct ttaaatttgt ttgagaaata tcctaaattt
    19261 gttaatggtt cttctgtcat tgctatgtcc ttactccttt tttatttatt gaaagcaata
    19321 aaaaataatg cctttttttt gacaaaactg agtttaagtt ttatcatagg aggtggaata
    19381 ggaaatatca ttgatagaag caaaaaggga tatgtggtag atttttttgc ctttaagatt
    19441 aaaaaatccc ccatttttaa tattgctgat atttttatta tctttggcgt gattctttta
    19501 caattaaccg ttttatttaa gaaaaatcca attgctggag agtaaagtga gggataaaaa
    19561 tgtcggatag ggtagcccag acaatatcta ccgagggatt aaagcccggg atggtactgg
    19621 cagaggatgt ttttaattcg gttggtttaa gactttttag tgaaggaacc aggcttgacg
    19681 acaagagtat tgcaaaaatc agaatgtacg aaatagattt tgttttaatt tatgaagatg
    19741 tcaatattga ttatgttaag gcgaaggttg aagtagaaag caaaagcatt agaaaagttc
    19801 atgagtttaa agcttttaag tcttcttaca taaaaaatgt taatacactt caagattacc
    19861 tgaccactat aggggaaggg aaaaatgtaa gtattacgga tcttttttcc ttaagccagg
    19921 aaatcgtatc agaagtacag accaaaagtg atattttagc ttttatgcat aatctgaaag
    19981 tctctgatga ccatacttat acccattctg ttaatgtgtc tttactttgc aatattttcg
    20041 gtcagtggct tggttttgac agtgaaaaat taaaaaatat tacagttgca ggtctgattc
    20101 atgatatcgg aaaaattaaa gttgatccca atatattgaa taaacctggg aagcttaccc
    20161 ctgaagaatt tgaagaagta aaaaaacatc cggtttatgg ctatcggatg atagaaaaaa
    20221 cggatatgca caaggatatt aaactggctg ttttaatgca ccatgaaaaa tatgacggct
    20281 cgggttatcc gacaggtgca aaaggtaatc aaattaacga tcttgcaaaa attgtagcca
    20341 tagccgatat ttatgatgcc atgacttcta acagatctta cagagataga ttttgcccct
    20401 ttaaggtgat tcaaactttt gaacaggaat gctatggaaa gttagataca aagtatttgc
    20461 ttatttttct gaaaaatatt gcttataact atttagattg ctgggtaagg ctttcttcag
    20521 gtgaagaagg gcaaattgta tttatcaatc aaaataacct ttcaagaccg attgtttcta
    20581 tcgataatgt tttggtggac ttaagcatgg aaaaagattt gaccattgaa gaactgatat
    20641 aaaagggtaa taaaaaatac aaattattac taaaatacat attttagcta cttgattttt
    20701 gttataaata tgatataata aagttaacca taatcacatt tgtttcctgg gatgaccgaa
    20761 aacctggtat tttgaaccta caccgttaat atgggagttc tatatcctgg agttaatgtc
    20821 aggcctcatt tttttccctt tgggacagag gaaggcagag gttccagtga ggacacccac
    20881 ctggcatgag ctagggcgtc aaaaatcagg gaaacggcat tttgggtaac aagtatcaaa
    20941 gaaaaacctg cacgtatgtg caggtttttc tttttgcttt ataaagaggg ataagaggaa
    21001 gaggattatt taaataaatt cataaattct tccagggatg aaaagtcttt tttatagcgg
    21061 tagaaagata gaaggtattt ttcatctact aagtccctta tggttttttc atcagctttt
    21121 tcaaaagcaa cgacaaacac atcacttttt gtaaggaaat aaatatcttt atagttgtct
    21181 ttaatatctt ttggtacatc gatggcttca aaagaaaaat caggattatg gatatgtata
    21241 agggtcaaat ctaagaatag atcttcggct tcctggatgt gtttttctaa tgcatttaaa
    21301 aagatttttt catggtttgg ggtcaacagt tcagcctggt ttttaattcg taacagatga
    21361 tacaatacgg gtgcaatatg atttgcaatg gtgtcgacgg taagtaagct ttcctgatct
    21421 gaaagtatgg cattggacag ttcaaaaaca catataagtc ccagtaattt cgtttcggca
    21481 tcttctatac atataggaac aaataaggca ccagccccgt tttttataga ttcagcaaat
    21541 tctttatcaa gcagctgagg gattttttcg ttactgttaa taatgaaata atctccttct
    21601 ttcagacaag agaacttatc agacagtttg aaagaagcag gacagttttc aagatttaat
    21661 gactttataa cttcaaattt ttcattatcg tatagggcaa aaataccgga ttttatacca
    21721 aaagaaatat tcagggtgct taaagtcaaa ttagccagtt cttcggtggt tgccgcacta
    21781 ttgatatttt tcattaaatt atttaaagaa accagtctgt taaacttctt ttgaaggagc
    21841 aatttctgct cattggtttc tttaaagtat ttggcgttag aaacagcaat ataagttgaa
    21901 gaagcaaggc tttcaatgag ttcaaaaatg cctttgttat agggcttgtc tgttactttc
    21961 ttaccaaggg ttacaaaacc taaaagcacc ttatttttta ttaaaaggac aatgtagaga
    22021 ggtcttaaat ttttaataac cgcttcactg ttttggaaag aatccataaa taattgatag
    22081 tcttcctgaa tactcatatc taaaattgat tttgaataat caaagagggc atcatttttt
    22141 aagtagacag acattgtaag ttggttagaa ggattaaaag tatccctcat tcctcttaga
    22201 acataatttt ctcccttttc atcatataaa aagaaagaag taatagaact ttgagtcagt
    22261 tctgcaaaaa catcaataga aagattataa agcaccgtta aatccaattc gctaagaagt
    22321 accttagagg actgattaat cgcaaaaaga ttaaagactt tttcatccaa ttccattttt
    22381 gcagtttgca gactctgata gctcttataa ttatttaaag aggtattaaa aagctgcatc
    22441 agggcttctg caacaatgga atcttcttcg tcaaaggctg gcttatcttc aaaacggttg
    22501 gttaagataa atccatataa ttcactttct ataatcagag gaatagctaa ttttgatgga
    22561 aagtctgtca gaacttttga gggaagatac tcctctaaag catgactatt taagatatga
    22621 ccatgaaaca aagcaatttc atcatattta ggcgtatatt ccgttgaata ttgatggata
    22681 ttatagcctt ttgatttaag taaagtatat tgattttttt ctttataaaa aatagcaatc
    22741 tgatctacaa gaatcagttc gttcacaaag tcataagcaa attctaaaat ttgatcaatg
    22801 ctaaattttt gtgaaaaaaa ttctatagca tggattaaac ctgtatagcg atataattta
    22861 cttgtaatat ttgctttttg tttgtcatct ttaattttct ttagtacatc atcatagctc
    22921 atatctatcc tcccttctta gcgcccggat ttctaactgg attttaattc gttttcaagg
    22981 acgtaatatg taataggtcc tctggtttga tccataaatc tcttttgaat attttttatt
    23041 tcaaaagtcg tattgccatg aactatacca taaatggtta tctcaccttc acctttagca
    23101 cgcaggtaag ataaatagtt ttttctttct gcctgtaatc tttttaattc ttctcttaga
    23161 ttggagatat cttttaaaat tcttttaaat tcatattgat gt
//
LOCUS       DAOSUD010000031        22834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1471_length_22834_cov_20.761798, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000031 DAOSUD010000000
VERSION     DAOSUD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 22834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22834
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_22834_cov_20.761798"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..114
                     /locus_tag="PLG49_05585"
     CDS             10..114
                     /locus_tag="PLG49_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ16829.1"
                     /translation="MVKNNTGMTEEEARQMMQAFFPELKRWENESNSK"
     gene            complement(138..1238)
                     /locus_tag="PLG49_05590"
     CDS             complement(138..1238)
                     /locus_tag="PLG49_05590"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ16830.1"
                     /translation="MEKSKHYLHGGDLEAIEKTYAIPREQLIDFSSNVNPLGISPKAK
                     KELANHLDLISSYPDRKYISLRQKIAQFTGADFDSIIVGNGSTELISLFITTVRPKKA
                     LIIGPTYSEYEREILLAGGRIQYFPLKEELDFQLDSEHLYHVLSEDFDLLVLCNPNNP
                     TGSVLRHKELSDIINFCKNKNIYVMIDETYVEFAEDMEDVEAIPLTKQYDNIIILRGI
                     SKFFSAPGLRFGYGICKNEKLIHKMNEIKNPWTINILASFAAGIMMEDEEYIKKTKEL
                     IHSERKKIYETLLAWKNIKVYKPSANFVLFKLLRNDITSGEIFEKLIHQKMIIRDAGS
                     FPFLDTRFMRFCFLLPDQNEKLLKALYEIIEK"
     gene            1386..2582
                     /locus_tag="PLG49_05595"
     CDS             1386..2582
                     /locus_tag="PLG49_05595"
                     /inference="COORDINATES: protein motif:HMM:NF040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HOQ16831.1"
                     /translation="MKNIFYAIKSEEYNEEPPVLSVSVNELKLEIAAFSVYTGAFKIN
                     NEGKGKLKGRIISKNGYVTFNPEEFEANEQVIIYRIDTKELLKDNHYTDYWLIESNGG
                     EKEILADISISYPLLIIDDKNTIADLKEFASYAKNNWSNAKEIFFSPLFLSWIYHNND
                     TMVINIYKQVSELTDKDWALEYFFRLTALKQIPALWIKEDERNIELMPANKLPLLKTL
                     VLHKRGWGLLEGEIKTDVNWIRVKKNHFNLADFENNRLIIDYEILPDKIKNRVSFGKI
                     IIQYEDKEIIYHIKLLRKDELMFSFSKKSYKQEDVGVLIVNNYTGEDLLLEIIPKDPW
                     IIFEAKKYIIGKYAEIPFRVHMTRWHKMGLGKLPYYDTFIEILANTKRKRLKQKMIKI
                     INVLGS"
     gene            2594..5323
                     /locus_tag="PLG49_05600"
     CDS             2594..5323
                     /locus_tag="PLG49_05600"
                     /inference="COORDINATES: protein motif:HMM:NF040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HOQ16832.1"
                     /translation="MNAYIEERLDFYNQKLEESGQPTTAHHYNLIRVLLYWLAGEMES
                     SNRNAYFKNALKILGQLINENATDIRLSFASIYLNLKLEEYENAYEILKPLLKYKKYY
                     ENSNKIYYGLMHYFKALLDYYNGKHMFIQKSIKIFEASLEATRSPLLYFLKANLEKEL
                     FLEEKNEISFYYKAYKSGFRSPFLYHRVYQLFCSNPDQFYNMNDMLMAVFTWALRYDF
                     LDEKALLLFSERMLQTEESLDIPESYIRLAQKRWDNNNFLSLLCKKYIKKNKRDKEAF
                     NLFSEAIDKKLYVEGLYQAYAETAKNMDYSDIPVTSLHPIFLGSLEKDVLAYVYSLIC
                     KKTKYKSYYQINMKRVYDFGIQALKAGKKGKYYIDIYMKLLENNPNHTQLKKTVFEHL
                     FLYEVKVNSPFIKYLWIIENEKEKMTTYTISRDSLYIEAAADKKEDLTVLCVGQRQKS
                     FYPDKLLTIYKLIKDVPFELLLKYYDEDYKTTNLLIALTRYFIKMTRENIEISIPQKA
                     GKIMTECLENSSLSDSFRKETSAALGTVLAKNQQFDKAFLYFEPLSFNDLSPCQVEYA
                     IAALLEGGEINKAILWAQAANELSNEINFKLIKKAIEKKLIHSYVINTAYQLLIKGKY
                     DEEILNYVIENYQGSMEDWYRLRDHLAALDKSSLLLDKKILQKGIWTRSLTTKLEEVF
                     QSLCKRKSPISKAFVDYCNYEVLINKKILSDTTLMILEDVFEKTKSMILASSLIYNYG
                     KKNMDLTDKEEMIQGVYLWMKNNDFLLPIFKEKKDIFPYSAYIEQNVPFIYYTKGGRE
                     VYFCYKIKGFPLNKKKMYNIAFNLYGTAVSLFYNESMEYYIEETDEEGNILLTDVKLY
                     QHEIKNISKEPKEVYEKLNNAIIFNHESNFEKAEEILEELILEDKRNNVGYLL"
     gene            5343..7019
                     /locus_tag="PLG49_05605"
     CDS             5343..7019
                     /locus_tag="PLG49_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOQ16833.1"
                     /translation="MGTLGLDVERGLFLGIDFGTTNSVVSIYDFKEGQVETIPIDGSN
                     IFPTAIQFETDPEDPDKLSRIFGVQAKEAAIIYPESTVLSVKRLLGSDEKIKILVDGK
                     EYEFTPEQIAGEILGYLKQKADEYVQEELNIAGEFSGCVITVPANSTDKQKKMTKEAA
                     KLAGFDEDNIYLRLEPAAAAISYAVNERKDKKVLVYDFGGGTFDACILDIKMTEADEP
                     RITILSTYGINDLGGNDIDKIIMDMIYEEFKKMTNEEIDLFDYTVDDGVSKRQKTTAL
                     VRLAQVANQAKERLSSSKSTKVVLAPFIQEPKIVNINMEITREAFLNHKRVNPLGDPA
                     ELFEKMEGKSVEDLIERTLECVDECLKNASLKVEDIDEIFLVGGSSAIPRIREKITEK
                     FNKEPFKSKISPALSISQGAAHYCNMIMMPTVKGPVVQEKTIHPLGLEIAGRRFMEIV
                     KSGLDIPEEGLTVEAEELLMTNFDNVTSMAIVVYENTKPSAEKKTSYVTDEGMKRLAG
                     TSLRGIPPKAKGEEKVKVIFHISQDNMLKVTAKSMSTDGVETELSVDDLY"
     gene            7044..8978
                     /gene="grpE"
                     /locus_tag="PLG49_05610"
     CDS             7044..8978
                     /gene="grpE"
                     /locus_tag="PLG49_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ16834.1"
                     /translation="MLKDYPPVLLKSKGLVEAWRDTLQAFDKFIEENIRGCFFHIKMK
                     VMLRILHHLIEENKLSKYDEKIFEYYDHLMLHYKNSIELFYNNEEKVKTGKSKEILTG
                     ICDVLSAQLRQFKENQLASQGISDEKAIQNPIKVEKDNFLTEEKIDILNQLDALEKQW
                     TEKKMKACVASFRKYINIVTENEDKEIELIEKIYEALLKDLKDALYTGYVKKTNKGIK
                     KLNDFHLRKAANFYYESIKQEKENIEAIIKIQVKALEDEMEKENYDKEEEQIIQEILH
                     TIREAYQHLGKEIEELELFFKESEEDKNKIVLFTSEEFDEYLKEQGLKSYINDIQVRK
                     KLKLEVDEPTQARKNFDFVKQIWEELKADLLKIYLEKINLEAFIGEMNQRLQNNIELS
                     SRVNQLFVQFLRTYEEQKEKFNEESGYLPIIEGIYETISIKVESIEENITGFHKTIEE
                     INLFFSEEASDAYFDSEFIKLAQEIFAQFIKEPAGENFEEESFLSWGEEYFNKAFEEA
                     FIRFSQKVEMVKEKLCQEVNLQINKFLKEYLLFEVSTYEEIVNYSVSRLREESDDFAI
                     EYVKNIDQLTASLEETLKEFEIEFVEPVPHDKFNGKEHEVLLAEEREGFKKGEIIKTL
                     NKGYRFNNQIILKANVVASK"
     gene            8993..10570
                     /locus_tag="PLG49_05615"
     CDS             8993..10570
                     /locus_tag="PLG49_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOQ16835.1"
                     /translation="MSWKLFKKELALKELDDETKFILGLDIGTRATKIAIWNVNRKQA
                     ELIDMSGGYGTPSIPTVMQYIPENKEWVFGEYALLNKGDFDDITINNIMSKLGTKELI
                     EIGGKAMNIPSILARYIKEIIEMCKNLNPNGEVVGLVATVPDYFPESAREEFKRAFAM
                     AGLDKVLITFMTFRECILRYLYFGQTIDKEEKVLFIDYGGQQIRGGVIQVNPESKGRV
                     KAESLSFLFDASLGGKKLDKLLKELFIDFYCQETGLLRTQLDEDTIHQIEVFAYQNKY
                     LLFQNYYKKKPTKLYFNFAYPAFQKTLEVEQLDKLIEPFAKSIQDFISNLLEKTIHGA
                     VQKEEIQKVIAVGGGFEMNWVKELIEHEFMGIDIEYPRDSEGTLALGACIGAASYLGV
                     VPELMIEIEDYHKLTFDIGLEGIYEKQSRFIPLVERNSFWWQKARPRIFILDLGGSNF
                     LEIPIYAKNEQGEIKTLEVIKLDNLPRNPSRTLRLKFDLKFSQYDTIELSIKDCGFGE
                     IIPPSDFSKTFNIQLSW"
     gene            10612..12252
                     /locus_tag="PLG49_05620"
     CDS             10612..12252
                     /locus_tag="PLG49_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOQ16836.1"
                     /translation="MTWEDYKREIEEKGSLPEEWYILGIDLGTTNSVISYWDNVNKKP
                     EPIDISNGFGKIPLPSVVQYREGEGQEGEWVIGEEAYRSMKIYPETTIRSIKRKMGTN
                     EKVKIGDKEYLPEEISAMILKELISHCNSINPNAEIIACVVSVPYDFDDAAKKATMKA
                     CELAGLKDKFICLIEEPKAAALAYNFRHNLSQDEKIMVFDFGGGTLDITLFHVVEKDD
                     YRIKLQVISEGGEAYHGGDNIDETLLEQCYKYIEEKTGQTRENISLENQAELVMRARE
                     TKERLSGVKSFRIPFTFCIPPFVEQINRDQFEELIHPFIEKTRKLVLKSLREAYTGAL
                     TADDIDRVLLEGGSSQMPWVKDMLIGIFNDDNKIYTSERPALDISLGATYYAAMKMGL
                     LTHPDLETEKMEVEFEVTVPHDIGLEVDHGSRKSFFTMIRRGTPYTLAKKSHVFTLSG
                     KNPQDMTTLNLRILERIDKEDTIENCKLIGEVEIQGLPERPSGKTKLQVTLSVEEEGG
                     LVKGMVEDLGFGEEYPPSGYKQSFDPSRFNKTVLEGEE"
     gene            12249..14324
                     /locus_tag="PLG49_05625"
     CDS             12249..14324
                     /locus_tag="PLG49_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOQ16837.1"
                     /translation="MSYLGIDLGTTNSVAVIYKDKNDEMEVVKVDGVDEILPSVVYYG
                     EEGVIVGSEAKAGAIIYPENTVISVKRFMGQTEAIKVGSTEKLPEEISAEILKKLKKA
                     AEDQSGETFDEVVITHPAYFNDRQIFATKKAGELAGFKNVHLLSEPLAAAIEYGYRQG
                     YAQTLLVYDLGGGTFDACVLKVSQDESGQEIFQELSDVGDMNLGGDDFDSELIRLMKE
                     RFTAESGIDIDKLETLERKRVLQKLKQEAEQAKKKLSGTNKVAIRINPLLIHEGVPKN
                     LSMELTREEFEALIRKYVDRSREIIAEALKRAGKEPDEISKVILVGGSTLIPMVKRMV
                     GGYIKEPYRATDPAKSVAMGAAIYNYLIHLPNSTVKVGQITRQIFGTEAIVNLATMEK
                     KLIPIIPMGSPIPVRISDSNFASMTGASSVQVDVYQWEQDHEEDRKYIGSVILTGLSG
                     TTQLEITYAIDKNNLFEVYVKDLSTGKTERAEFDRTKVLPPPRKEAKAVAEKMNIVFI
                     IDTTGSMDTYINGVKDRAIEFSNILRGKGIQFQLGLIGFGDLREKENPSVYNFTNDVE
                     KFQRQVKNIPRTYGGDIPESSLDAVLTGVELLQTSKVDKSGKNIFILITDAPPHVPTE
                     NGKHVAEIVEILKENNITTYVVARKDKNSIESFDPLTKPDGKYYDLQDKFYDILDNIA
                     ISIAELVRL"
     gene            14337..15500
                     /locus_tag="PLG49_05630"
     CDS             14337..15500
                     /locus_tag="PLG49_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ16838.1"
                     /translation="MSSLILSVSPENVERPYYFDMTRVRVYSIEELYYHCYHYWKQSI
                     DDFIMGKLEDWIRIELGLSHISGQIAKIKDEYSSITEQYIRFLGLIDYFYDSELESLR
                     KEIYKWEHKAEWEKCKEKADYFMAQEKPEEAMKWYMKAIVFDENPKIYNNMGVASMHM
                     GWYRDAAYYFQKAYQKEPNSIPILLNLTDVYILQKNFKEAGKYLSQAAQLDDSQVIWY
                     YYGELYNSMGNQKKAIEAYLKAISKGDHFDSYIKLARIYVQEKDFETAQAYLEKIKTS
                     QRNEIYWMEYSKLYEAWDKKDLALEAVQKSISKNKNYIYSWIALARFQRRNNCLEEAE
                     KAISEALRIDAENEEAQIEAAKIQKELGKEKEYRQSLKELINKWVHRYRQDTI"
     gene            15635..16591
                     /locus_tag="PLG49_05635"
     CDS             15635..16591
                     /locus_tag="PLG49_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOQ16839.1"
                     /translation="MGSIANTIKLYLILQSKGKVKIKELAEELEVDERTIRRYKDELE
                     QANIYIDSEPGRYGGYYLYNDNYLMGLTLSQEEYLALMMINEELSRSNHVVSRDYNSA
                     IEKISVVYNVHHHNATGTNFSNYTVKKSKSNVNISEERKKLIDIHAAALTRNKVKIKY
                     YSLSSGLSERIVHPYATIQYKGDLYFAGYCEKRQEIRDFKLCRIREYTVLDSKFPKND
                     NFNLEEYMKNCIGIIKDEKIDLKIQIFFPMAQIVKEKIFVDNQKITEYKDEGYIIYEA
                     TMEGLEEIKSWILSMGSYAKVLEPKFLQDEIKDEIKKIINIY"
     gene            16689..17408
                     /gene="cas6"
                     /locus_tag="PLG49_05640"
     CDS             16689..17408
                     /gene="cas6"
                     /locus_tag="PLG49_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOQ16840.1"
                     /translation="MTKNKIPLDYRRIFISLFKKALEQYNTDSKKKYYHENDPIKKAF
                     TFSVFLDRPKFYKDYIELKQNKIDLYFSTYSYEIGIDYYNALIKMLNYAYPLQDDNYL
                     ILKNVASIKEANIKQDSILIKTMSPILARKHDKNRNYDTYLLPSQEGFIEEMRNNISV
                     ALESLDLNDLKEDVQSFELIDIGKGKEVKVLFYGHKVLGLIGNYFLKGTPRLLEFVLK
                     SGIGSRTSSGFGMFDVEGVSR"
     gene            17405..19081
                     /gene="cas8a1"
                     /locus_tag="PLG49_05645"
     CDS             17405..19081
                     /gene="cas8a1"
                     /locus_tag="PLG49_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HOQ16841.1"
                     /translation="MNEITIQLGDWLFNAGVVGLVNILLHAGDEVKLNGQEVSFNAGV
                     LDKFEEKYFKYFIDTYEKTLAWYKVVSFEDFIKKHEEENFESFTEKDLEILNQYIGSG
                     SKSGTLKYILTSNSHKAAYNLITSELDVLELEKQISTIKLKKNEELKDQIPKIKDLMK
                     KIHDIIRYYNQPDAKKYIAAKNVIYTIINNAWNNVSFLNRQTKIPDMYEDYKIYFLQG
                     LNDYLNNNKKEYNYSCSNCGSLMNDMNNDIAFLNATGFDTSRKLSNVWNFNNDIAICP
                     VCKIVYSCVPAGFTYVYDKGIFINQNQDIQSAVNVNQRIKSEIIDSGDVKSIWEFYRI
                     VSEEMERKINEGVKYELADIQVVRYENESYRFNLLSKKMLKVLSKSTKELNALLKLGY
                     KEVNTYFNIYDEVMKRLLNNENLFLLIHKLMIQKLTKPSESYYQMRNINYLLEINNNY
                     LEGVVGMEVKERDILELSKNAGYYFRKAYEERNVENKINGIAYRLLNALKTNNQHMFM
                     DTLINCYLYIKQSVPKVFIDGMKDDYEFKSVGYAFVAGMTSESKKNEENN"
     gene            19099..19986
                     /gene="cas7i"
                     /locus_tag="PLG49_05650"
     CDS             19099..19986
                     /gene="cas7i"
                     /locus_tag="PLG49_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HOQ16842.1"
                     /translation="MSNSTLKPKGLTISMIFEAESANYGEGIGNVASLKKVARGNGEQ
                     YTYISRQAIRYNIIEQLGEELAKVQAEGSGDKKVIQFAPDSTIKDYPEIDFFGYMKTK
                     KGEGSVTRSAKVRLSNAVSLETFKGDLDFLTNKGLADRLNENMNLAQSEIHKTYYRYT
                     ITIDLDQIGIDAQDNIELDNQEKARRVNKLLDTISMLYRDIRGRREDLKPYFAIGGVY
                     DIKNPVFQNALEVRNNRVQISLIEGVMYDFIKNDTLCGLVEGKFLNDSEIKERLNAIP
                     MPEFFSKIKEKVSEYYESC"
     gene            19973..20722
                     /gene="cas5b"
                     /locus_tag="PLG49_05655"
     CDS             19973..20722
                     /gene="cas5b"
                     /locus_tag="PLG49_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HOQ16843.1"
                     /translation="MKAVRLKLSQELVNYKVPTSFQLKETYPLPPYSTVIGMVHNMCK
                     YTEYKEMKISIQGKYLSKVNDLYTRYEFKPECPFEKGRHQLIINGFGITKGIATAELL
                     TEVELLIHVIPEDQSIIREIESAFLMPWEYPSLGRREDLVVIDEVKVVELTEKMFEKS
                     MSSNKDYSAYVPIDLMDNIKFGNRQQGIEFVGTRYKLNKNYSLQTIGKGKSKKEFRRW
                     NKINVVYGSNIYGIMKKTLLIDEDNYFVFPA"
     gene            20730..>22834
                     /gene="cas3"
                     /locus_tag="PLG49_05660"
     CDS             20730..>22834
                     /gene="cas3"
                     /locus_tag="PLG49_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOQ16844.1"
                     /translation="MININNYWAKSNPKETIQEHTDSLLKNLCLFKSIYPNIPVNYEL
                     LKLACIYHDLGKMNIRFQAKIQASIDGKKSYKDKLEIPHGILSLSFINYKYLEDKGFT
                     EDEIRILFHAVAYHHDRDLDFEEEDLGREISLLKKECVGFKYDKADELIQLPDEIEAK
                     FYMLNDRIYEKNDDDNNNQTFYDYIKIKGLLNRLDYAASAGINVEVANDFLIQGLEKL
                     KEKENWGEWNKLQQYMINNRDNNVVVIAQTGMGKTEAGLLWIGNNKGFFTLPLKTAIN
                     EIYNRITKKIVPDREKVGLLHSETIFKYHELQESIEELDIDEYYLKTRQLSLPITVCT
                     LDQLFDIVFKYRGFEAKLATLSYSKIVIDEVQMYSPDLLAYLIVGLHYIIKLGGKFAI
                     LTATLPGIVKYYLKGEGIEFQEPQSPFIKDELIRHSVKVIPSQINAEFILQHFNNNKV
                     LVICNTVKKAREIFEDLNNNKKLFDKDVTIKLLHSRFTRKDRREKEEEIVEFGSKNSM
                     ETGIWVATQVVEASLDIDFDILITELSDLNGLFQRMGRCYRHRTYDKQGYNCFIFDGG
                     ERTCSGVGSVIDEDIFELSKEAINDIDGIMKESEKMQLVEKVYSAEKLMGTKYYQEVQ
                     KTINYIKTITEFEKSKDDINFRNIDNITILPKCVYLNNKAEIDELINIYKDEKTTKDD
                     KRKCWIDIQDYLVNVPGYEV"
BASE COUNT         8520 a         3136 c         4485 g         6693 t
ORIGIN      
        1 gttccgcatg tggtaaagaa taatacggga atgacagaag aagaagcccg acaaatgatg
       61 caggcttttt ttcctgaatt aaaacgctgg gaaaatgaat caaacagtaa ataaaaggtg
      121 ccaaatagag gcacctttta tttttcaata atttcatata aagcttttaa taatttttca
      181 ttttgatcag gaagtaaaaa gcaaaatctc ataaatcgtg tatcaaggaa aggaaaactt
      241 cctgcatctc ttatgatcat cttttgatga attaactttt caaatatctc tcctgatgta
      301 atatcatttc ttaaaagttt aaagaggaca aaattggcac tgggtttata gaccttgatg
      361 tttttccatg ccaataaagt ttcatagatt ttttttcttt cagaatgaat gagttctttg
      421 gtttttttaa tgtattcttc atcttccatc ataatgcctg cggcaaaaga agccagaata
      481 ttaattgtcc agggattttt aatttcattc attttatgga taagtttttc atttttgcat
      541 ataccatatc caaagcgtag tcccggagct gagaaaaatt tagaaatacc tcttaaaata
      601 atgatgttat cgtattgttt tgttaaggga atggcttcta cgtcttccat atcttctgca
      661 aattccacat aggtttcgtc aatcatgaca tagatatttt tatttttaca gaaatttatt
      721 atatctgaaa gctctttatg cctgaggaca gaaccagtgg gattattggg attgcataaa
      781 accagtaaat cgaaatcctc agacaataca tgatatagat gttctgaatc taattgaaag
      841 tccaattctt cttttagagg aaagtattga atccttccgc cagccaataa gatttccctt
      901 tcatactcgg aataggttgg tcctataatt aaagcttttt ttggacggac tgttgtaata
      961 aaaagagaaa taagttcagt agatccatta ccaacgataa tggaatcaaa atctgctccg
     1021 gtaaattgag caattttttg gcggaggctg atgtattttc tgtcaggata gctggatatt
     1081 aaatccagat gattggccaa ttctttttta gcctttgggg aaatgcccaa aggattgaca
     1141 ttagaactaa aatcaatcaa ttgttctctt gggattgcgt atgttttttc tatagcttcc
     1201 aaatccccac cgtgtaaata atgctttgac ttttccatat tatctccttc tttatctgaa
     1261 aatgctttct taattttaaa aaaataaaaa agaatatact attatcagcg attatttatt
     1321 ggacaagtaa aaagattgat atataataat aaatgataat gagaaaaaag taaaggagga
     1381 ttagcttgaa aaatatattt tatgcaataa aaagtgaaga atataatgaa gagcctcctg
     1441 tactttcagt atctgttaat gaattaaaat tagaaatagc tgctttttct gtatatactg
     1501 gcgcgtttaa gataaataat gaaggaaaag gaaagcttaa aggcagaatt atttcgaaaa
     1561 acggttatgt aacttttaat ccggaagaat ttgaagccaa cgaacaagtc atcatttacc
     1621 ggatagatac aaaagaactg cttaaggata atcattatac agactattgg cttatagaat
     1681 ccaatggagg ggaaaaagaa attttagccg atatttctat tagttatcct ttattaatta
     1741 ttgatgataa aaatactata gcagatttaa aagaatttgc cagctatgca aaaaacaact
     1801 ggagcaatgc aaaagaaatc tttttttcac ctttattttt atcctggatt tatcataata
     1861 atgacacaat ggtgattaat atttataaac aagtttctga attaacggat aaggactggg
     1921 cgctggaata tttttttaga ttaacggctc ttaagcaaat tcccgctttg tggattaaag
     1981 aagatgaaag aaatattgaa ttaatgcctg caaataagct tcctcttctt aaaacactgg
     2041 tgcttcataa aagaggctgg ggcctgttag aaggagaaat aaaaaccgat gtaaactgga
     2101 taagggtaaa aaagaaccat tttaatctgg cggattttga aaacaaccgc ttaattatag
     2161 attatgagat attgccagat aaaataaaaa atagagtaag ctttgggaaa atcattattc
     2221 aatacgaaga caaggaaatt atttatcata ttaagctgtt aagaaaagat gaattaatgt
     2281 ttagtttttc aaaaaagtct tacaagcagg aagatgtagg agttttaatc gtaaataatt
     2341 atacggggga agatttactt ttagaaatta ttccaaagga tccctggatt atttttgaag
     2401 ccaaaaaata tatcattggg aaatatgcag aaattccttt tagagtccat atgaccagat
     2461 ggcataaaat gggcttggga aaacttcctt attatgatac ttttattgag attttagcaa
     2521 atacaaaaag aaaaagatta aagcaaaaaa tgatcaagat aataaatgtc ctgggaagtt
     2581 aaggagaagt tatatgaatg catatataga ggaaaggttg gatttttata atcaaaaatt
     2641 agaagaatcc ggccagccga cgactgcaca tcattataat ttaataaggg tgctgttata
     2701 ctggcttgcg ggggagatgg agtcctctaa caggaatgct tattttaaga atgccctgaa
     2761 gattttaggt cagctcatta atgaaaatgc tactgacatc aggctttctt ttgccagtat
     2821 ctatttaaat ttaaaattag aggaatatga aaatgcctat gaaatattaa agcctctgtt
     2881 aaaatacaag aagtattatg agaattcaaa caagatttat tatggtctta tgcattattt
     2941 taaggcttta ctggattatt ataatggcaa gcatatgttt atacaaaaat caataaaaat
     3001 ctttgaagcg tctttagaag caacccggag tccgcttttg tattttttaa aggccaattt
     3061 agaaaaagag ctttttctgg aagaaaaaaa tgaaatcagt ttttactaca aagcctataa
     3121 aagtggtttt agaagccctt ttttatacca tagggtttat cagttgtttt gcagcaatcc
     3181 agaccagttt tataatatga acgacatgct tatggcagtt tttacatggg ctttgcgcta
     3241 tgatttttta gatgaaaaag ccctcttact cttttctgaa aggatgctcc agacagaaga
     3301 aagtctggat attccggaaa gctacataag attggctcaa aaaagatggg ataataataa
     3361 ttttcttagt ttgttatgta aaaagtatat caaaaagaat aaaagagaca aggaagcctt
     3421 taatcttttt agtgaagcga ttgataaaaa actatatgta gaaggacttt atcaggctta
     3481 tgcagaaaca gcaaaaaata tggattattc cgatattcca gtgacatctc ttcatcctat
     3541 ttttttagga agtcttgaaa aagatgtttt ggcctatgtt tattccctta tttgcaaaaa
     3601 aacgaaatat aaaagttatt atcaaatcaa tatgaaaaga gtatatgatt ttggcattca
     3661 ggcattaaag gccgggaaaa aaggaaagta ttatattgat atctatatga agcttttaga
     3721 aaacaatccg aatcataccc aacttaaaaa aacagtcttt gagcacttat ttttatatga
     3781 agtcaaggtg aattctccct ttattaagta tctctggatc atagaaaatg aaaaagaaaa
     3841 gatgaccact tacaccatta gcagggatag cttatatata gaagcagctg cggataaaaa
     3901 agaagattta acggttctgt gtgttgggca gagacaaaaa agtttttatc cggataaact
     3961 tttaaccatt tataagctta taaaagatgt tccctttgaa ttgcttttaa aatactatga
     4021 tgaagattat aaaacgacta atttacttat tgcccttacc agatacttta ttaagatgac
     4081 cagggaaaat atagaaataa gcattcctca aaaagctgga aaaataatga cggaatgcct
     4141 ggaaaacagc agtctttctg acagctttag aaaagaaact tctgcagctt tagggacggt
     4201 tctggctaaa aatcaacagt ttgataaggc ttttttatat ttcgagccct tgtcctttaa
     4261 tgacttatct ccttgccagg tagaatatgc tattgccgct ttactggaag ggggagaaat
     4321 aaataaggca attttatggg cacaggcagc caatgaatta tccaatgaaa taaactttaa
     4381 gctcattaaa aaggccatcg agaaaaaact catccattct tatgtgatca atacggctta
     4441 tcagcttctt attaaaggaa aatatgatga agaaatctta aactacgtta ttgaaaatta
     4501 tcagggcagt atggaagact ggtaccggct cagagatcat ttagcagctt tagacaaatc
     4561 aagtctttta ctggataaaa aaattcttca aaagggtatt tggacaagaa gtttaacgac
     4621 taaacttgaa gaagtctttc aaagtttatg caaaagaaaa agtcctatat ccaaagcttt
     4681 tgttgattat tgcaattatg aagttttaat taataaaaag attttgtctg atacaaccct
     4741 tatgattttg gaagatgttt ttgaaaaaac caaaagtatg attttagctt cttcccttat
     4801 ttataactat ggaaaaaaga atatggacct gacggataaa gaagagatga ttcagggagt
     4861 ctacttgtgg atgaaaaata atgactttct tcttccaatt tttaaagaaa aaaaagacat
     4921 ttttccgtat tcagcgtata tagaacaaaa tgtgcctttt atttactaca ccaaaggagg
     4981 gagagaagta tacttttgct ataaaattaa gggtttcccc ctgaataaaa agaagatgta
     5041 taatattgct tttaatttat atggaacagc agtttcctta ttttataatg aaagcatgga
     5101 gtattacatt gaagaaaccg atgaagaagg caatatttta ttgaccgatg taaagcttta
     5161 tcagcacgaa ataaaaaata tctctaaaga acctaaagaa gtctatgaaa aattaaataa
     5221 tgccataatc tttaatcatg aatcaaattt tgaaaaagca gaagaaatat tagaagaact
     5281 gattcttgaa gacaaaagaa ataatgtagg ttatctatta taataacaag gaggatgaat
     5341 gcatgggaac attaggtttg gatgttgaaa ggggattgtt tttaggtatt gattttggta
     5401 caaccaactc tgtggtaagc atttatgatt ttaaagaagg gcaagttgaa accatcccta
     5461 tagatggaag taatatcttc ccaacagcca ttcagtttga aacagatcct gaagatccgg
     5521 ataaattatc cagaattttt ggggtgcagg ctaaagaagc tgccatcatc tatccggaaa
     5581 gtactgtttt atctgtaaag cgcctgcttg gaagcgatga aaaaattaaa attctggttg
     5641 acggcaagga atatgaattc acgcctgaac agattgccgg ggaaattttg ggttacctaa
     5701 aacaaaaagc cgatgaatat gtacaagaag agttaaatat tgccggggaa ttttctggtt
     5761 gtgtgattac ggtaccagcc aactctacag acaagcaaaa gaaaatgacc aaagaagcgg
     5821 caaaactggc tggctttgat gaagataata tttatctgcg tctggaacct gcagcagcag
     5881 cgatttctta tgcggtaaat gaaagaaaag ataaaaaggt attggtttat gattttggtg
     5941 gaggtacttt tgatgcttgt attttggata taaaaatgac cgaagccgat gaacccagaa
     6001 tcactatctt aagtacttat ggtatcaatg atttgggcgg aaatgatatt gacaaaatca
     6061 tcatggatat gatttatgaa gaatttaaaa agatgaccaa tgaagaaata gacctgtttg
     6121 attatacggt tgatgatggg gtatctaaaa ggcaaaagac aacggcttta gtcagactgg
     6181 ctcaggtagc taaccaggca aaagaaagac tttcttcatc taaatccact aaagtagtac
     6241 tggcaccttt tatccaggag cctaaaattg tcaatatcaa tatggaaatc acaagggaag
     6301 ctttcttaaa tcataaaaga gtgaatcctt taggagaccc tgcagaatta ttcgaaaaga
     6361 tggaaggaaa gtccgtagaa gatttaattg aaagaacgct ggaatgtgta gatgaatgtt
     6421 taaaaaatgc ttctttaaaa gttgaagata ttgatgaaat ttttcttgta ggtggttctt
     6481 cagcgattcc aagaatcagg gaaaaaatta ccgagaaatt taataaagaa ccctttaaat
     6541 ctaaaataag cccggcttta agtatttccc agggtgcagc ccactactgc aatatgataa
     6601 tgatgcctac ggtaaaaggc cctgtcgtac aggaaaagac cattcacccc ttaggacttg
     6661 aaattgccgg aagaagattt atggaaatcg taaaaagcgg tctggatata ccagaagaag
     6721 gtcttaccgt agaagcagaa gaattgctca tgaccaattt tgacaatgtg accagtatgg
     6781 ctattgttgt ttatgaaaat accaagcctt ctgcagaaaa gaagacctct tatgtaacag
     6841 atgaaggcat gaagcggctt gcaggaacaa gtttaagagg gattcctcct aaagcaaaag
     6901 gtgaagaaaa ggtgaaagtc attttccata ttagtcaaga caatatgctt aaggttacag
     6961 caaaaagtat gagtactgac ggagtggaaa ccgagctgtc cgtggatgac ttatactaac
     7021 ggccagtaaa aggaggatta aggatgttaa aggactatcc ccctgtttta ctaaagtcaa
     7081 agggattagt ggaagcctgg agggacacac ttcaggcttt cgataaattt attgaagaaa
     7141 atatcagggg ctgttttttt catattaaga tgaaggtgat gctacgaatt ttgcatcatt
     7201 taatcgaaga aaacaagctt tctaaatatg atgaaaaaat ttttgagtat tatgaccatt
     7261 tgatgcttca ttataagaat tccatagaac ttttttataa taatgaggaa aaggtaaaga
     7321 cagggaaatc caaagagata ttaaccggga tttgtgatgt tttatctgcc cagttgagac
     7381 aatttaaaga aaatcagctg gccagtcagg gtataagtga tgaaaaagcc attcaaaatc
     7441 ccataaaagt tgaaaaagac aattttctga cagaagaaaa gatagatatc ttaaaccagt
     7501 tggatgcatt agaaaagcag tggacagaaa agaaaatgaa ggcttgtgtt gcttctttca
     7561 ggaaatatat aaatattgtg acagaaaatg aagataagga aattgaactg atagaaaaga
     7621 tttatgaagc tttattaaaa gatcttaaag atgctttata tacgggttat gttaaaaaaa
     7681 ccaataaagg cattaaaaag ctaaatgatt ttcatttaag aaaagcagcg aacttttatt
     7741 atgagtctat aaaacaagaa aaagaaaaca ttgaagccat tatcaaaatc caggtcaaag
     7801 ctctggaaga tgaaatggaa aaagaaaatt atgataaaga ggaagaacaa atcatacaag
     7861 aaatccttca taccataaga gaagcttatc agcacctggg aaaagaaata gaagaactgg
     7921 agttgttctt taaagaatcc gaagaagata aaaataaaat tgtccttttt accagtgaag
     7981 aatttgatga ataccttaaa gaacaaggtt taaagtctta tatcaatgat attcaggtaa
     8041 gaaaaaaact taaacttgaa gtggatgaac caacacaagc gcgtaaaaat tttgatttcg
     8101 ttaagcagat ctgggaagaa ttaaaagcag atcttttaaa aatttatctt gaaaaaataa
     8161 atcttgaagc ttttattggg gaaatgaacc aaaggcttca aaacaatatt gaattatctt
     8221 caagggtcaa tcagttattt gtacagtttc ttagaactta cgaagaacaa aaagaaaagt
     8281 ttaatgaaga aagcggatat ttacccatta tcgaagggat ttatgagacc attagcatta
     8341 aagtagaaag tatagaagaa aatattactg gttttcataa gacgatagaa gaaatcaatc
     8401 tgttcttttc agaagaagcc agtgatgctt attttgatag tgaatttata aagctggccc
     8461 aggaaatttt tgctcaattt attaaagagc ctgccgggga aaactttgaa gaagagagtt
     8521 tccttagctg gggagaagaa tattttaata aggcatttga agaagccttt atccgtttta
     8581 gtcaaaaggt agaaatggta aaggaaaaac tctgtcagga agttaatctg caaattaata
     8641 aatttcttaa ggaatatctg ctttttgagg tcagtactta tgaagaaatt gtcaactatt
     8701 ctgtatccag gctccgtgaa gaaagcgatg attttgctat agaatatgtc aaaaatatcg
     8761 accagcttac agcaagtctg gaagagactt taaaagaatt tgaaattgaa ttcgttgaac
     8821 cggtgccaca tgataaattt aacggaaaag agcatgaagt tcttctggcc gaagaacgag
     8881 aaggctttaa aaaaggagaa attattaaga ctttaaataa aggataccgt tttaacaacc
     8941 agatcatctt aaaggccaat gtggttgcaa gtaaatagtg gggatgagat ttatgagctg
     9001 gaaactgttt aaaaaagagc tggctttaaa agaactggac gatgaaacga agtttatatt
     9061 aggtctggat atagggacca gagcgacaaa gattgccatc tggaatgtaa accgcaaaca
     9121 agcagaactg attgacatga gcggaggcta cgggacccct tcgattccca ctgttatgca
     9181 gtatattcct gaaaataaag agtgggtttt tggggaatat gcccttttaa ataaggggga
     9241 ttttgatgac attaccatta acaatattat gtccaaatta gggaccaaag agcttattga
     9301 aattggcggc aaagccatga atattcccag tattcttgcc agatatataa aagaaatcat
     9361 tgaaatgtgc aaaaacttaa accctaatgg tgaagtggta ggtttggtag caaccgttcc
     9421 ggactatttt cctgaaagtg ccagagagga atttaaacgg gcttttgcca tggctggtct
     9481 ggataaagtt ctgattactt ttatgacttt cagggaatgt atccttcgat atctatactt
     9541 tggtcagacc attgataaag aagaaaaggt tctttttatt gactatggcg gccagcaaat
     9601 ccggggcggt gttattcagg ttaatccgga aagcaaagga cgggttaagg cagaaagctt
     9661 atctttcttg tttgatgctt cccttggagg gaaaaagtta gataagttat tgaaggaatt
     9721 atttattgat ttttattgtc aggaaaccgg tttattaaga acgcagctgg atgaagatac
     9781 gattcatcag atagaggttt ttgcttatca gaataaatac ttactttttc aaaattatta
     9841 taagaaaaag ccgaccaaat tgtattttaa ttttgcttat cctgcctttc agaaaaccct
     9901 ggaagtagag cagttggata agttaattga acccttcgcc aaaagcatac aggattttat
     9961 ttcaaattta ctggaaaaga ccatccatgg ggcagtacaa aaagaagaaa ttcaaaaagt
    10021 tattgcagtt ggcggcggct ttgaaatgaa ttgggtaaaa gagctgatag agcatgaatt
    10081 tatgggtatt gatatagaat atcccaggga ttctgaagga accctggccc tgggtgcctg
    10141 tatcggggca gcttcttatt taggtgttgt tccagagcta atgatagaaa ttgaagatta
    10201 tcataagtta acctttgata tcggtttaga aggcatttat gaaaagcaga gcaggtttat
    10261 tccccttgtg gaaaggaata gtttttggtg gcaaaaagcc agacctagaa tttttatttt
    10321 agatttagga ggcagcaact ttttggaaat tcccatttac gcaaaaaatg agcaagggga
    10381 aattaaaacc ctggaggtaa taaaacttga taatttaccc agaaatcctt ccaggacctt
    10441 aagacttaaa tttgatttaa aattcagcca gtatgataca atagaattaa gtataaaaga
    10501 ctgtggtttt ggtgaaatta tccccccttc ggatttttca aagactttta atattcagct
    10561 gtcctggtag tatatgcata caaagatttt tgcaaaagaa ggagtgagaa cgtgacttgg
    10621 gaagactata aaagggaaat agaagaaaaa ggcagtttgc cagaggaatg gtatatttta
    10681 ggtattgact tgggaacaac gaactcagtc atcagctatt gggataatgt caataaaaaa
    10741 ccagaaccca ttgatatcag caatggtttt gggaaaatcc cacttccttc tgtcgtacag
    10801 taccgggagg gggaaggtca ggaaggcgaa tgggtgattg gtgaagaagc ctacagatcc
    10861 atgaaaatat atcctgagac caccatccgg tccatcaaaa gaaaaatggg aaccaatgaa
    10921 aaagtaaaaa ttggcgataa ggaatatttg cctgaagaaa tatctgccat gattttaaaa
    10981 gaactgataa gtcactgtaa cagcatcaat cccaatgcag aaatcatcgc ctgtgtggtt
    11041 tcagttcctt atgattttga tgatgcggcc aaaaaggcta cgatgaaggc ctgtgagctg
    11101 gcaggattaa aagataaatt tatatgcctg attgaagagc ctaaggctgc agctttagca
    11161 tataacttca ggcacaacct ttcccaggat gaaaaaatca tggtatttga ctttggggga
    11221 ggaacattag atatcacctt attccatgtg gtggaaaaag acgactacag gattaagctt
    11281 caggtcatca gtgaaggcgg ggaagcttac cacgggggcg acaacatcga tgaaaccctg
    11341 ttagagcagt gttataaata tattgaagaa aagacggggc aaacaagaga aaatatttcc
    11401 ctggaaaacc aggcagaact tgtgatgcgg gccagggaaa ccaaggaaag attatcgggt
    11461 gtaaaaagtt tcaggattcc ttttactttt tgtatacctc cttttgtaga gcaaattaac
    11521 agagatcaat ttgaggaatt aatccatcct tttatcgaaa agactagaaa actcgtctta
    11581 aagtctttaa gagaagctta taccggagct ttaacagcag atgatattga cagggttctt
    11641 cttgaaggtg gttcctcaca aatgccctgg gtgaaagata tgttaatagg catctttaat
    11701 gacgataaca agatttatac ttccgaaaga ccagccctgg atatttccct gggggcaact
    11761 tattatgcgg ctatgaagat gggacttcta acccatcctg atttggagac cgaaaaaatg
    11821 gaagttgaat ttgaagtgac ggttcctcat gatattggtc ttgaagtaga ccacggcagc
    11881 agaaaatctt tctttaccat gataagaagg ggaaccccat atactttggc gaaaaaaagc
    11941 catgttttta ccttgtcagg aaagaatcct caggatatga ccacattaaa tctcagaatc
    12001 ttagaacgga ttgataaaga agataccata gaaaactgta aactcatcgg ggaagttgaa
    12061 atccaaggtt tgcctgaaag accttcagga aaaacgaaac ttcaggtaac cctttcggtt
    12121 gaagaagagg gcggtcttgt taaaggaatg gtggaggatt taggttttgg agaagaatat
    12181 cctcccagcg gttataagca gtcctttgac ccaagcaggt ttaataagac agtattggag
    12241 ggagaagaat gagttattta ggaatcgatt taggaactac caattcagta gccgtaatat
    12301 acaaggataa aaacgatgaa atggaagtag ttaaagtcga tggtgtggac gaaatcctgc
    12361 cttctgtggt ttactatggg gaagaaggtg taatcgtcgg gtccgaagct aaggcaggcg
    12421 ccattatcta tcctgaaaat acagtgattt ctgttaaaag atttatggga cagacagaag
    12481 ccattaaggt aggcagtacg gaaaaacttc cggaagaaat cagtgcagaa attttaaaaa
    12541 agctcaaaaa agctgctgaa gatcaaagtg gggaaacttt tgatgaggtg gttatcaccc
    12601 atcctgctta ttttaatgac cggcaaattt ttgctaccaa gaaagcggga gaattggcag
    12661 gttttaaaaa tgttcatctc ttatcagaac ctttggctgc tgccattgaa tatggttatc
    12721 gtcaggggta tgctcaaacc cttttggtat atgatttagg tggaggaacc tttgatgcct
    12781 gtgttctgaa agtatcccag gatgaaagcg gtcaggaaat attccaggaa ttatccgacg
    12841 ttggggatat gaatcttggg ggcgatgact ttgacagcga gcttatccgc ctgatgaaag
    12901 aaagatttac tgccgaatca gggattgata tcgataaatt agagacttta gaaaggaaaa
    12961 gagtgcttca gaaattaaaa caggaagcgg agcaggctaa aaagaaatta tcaggcacga
    13021 ataaagttgc cataagaata aatcctttgc taatccatga aggggttcca aaaaacttat
    13081 ctatggaatt aacccgggaa gaatttgagg ccctgatccg taaatatgta gacagaagcc
    13141 gggaaattat tgcagaagct ttgaaacgtg caggaaaaga acccgatgaa atttctaagg
    13201 ttatcctcgt tggaggttct acccttattc ctatggtaaa aagaatggta gggggttata
    13261 ttaaagagcc ttacagagca acagaccctg caaaaagcgt ggctatgggt gctgccattt
    13321 ataattattt aatccacctg ccaaacagta cggttaaagt tggacaaatt accagacaaa
    13381 tctttggtac ggaagccatc gtcaacctgg cgaccatgga aaagaaactg attccgatta
    13441 ttcctatggg cagtcccatt cctgtaagga tttctgacag taactttgcc agcatgaccg
    13501 gggcatcttc tgtgcaagtg gatgtgtatc agtgggaaca agaccatgaa gaagacagaa
    13561 aatatatagg aagcgtgatt cttacaggtc tttcaggtac aacccagctg gaaataactt
    13621 atgccattga caaaaacaac ttatttgaag tttatgtaaa agatttgtca acaggcaaga
    13681 ccgaaagagc cgaatttgac agaaccaaag tactgcctcc gccaagaaaa gaagcaaaag
    13741 ccgtagcaga aaagatgaat attgtcttta ttattgatac cacaggcagt atggatacct
    13801 atatcaatgg tgtaaaagac agggcgattg aattttccaa tattttaaga ggaaaaggca
    13861 tacagttcca gttaggtctg attggttttg gagatttaag agaaaaggaa aatcccagtg
    13921 tctataactt tacaaacgat gtagaaaagt tccagagaca ggttaaaaac attccgagaa
    13981 cttatggagg agacattcca gaatcctctc tggatgccgt tttaacagga gtagaacttt
    14041 tacagacatc caaagtagac aagagcggga aaaatatctt tatcttaatt acagacgctc
    14101 cgcctcatgt acctacagaa aacggcaaac atgtagccga aatcgttgag attttaaaag
    14161 aaaacaatat taccacctat gtggtagcca gaaaagataa aaacagcatc gaaagcttcg
    14221 atccacttac aaaaccagac ggaaaatatt atgatctcca ggacaaattt tacgatattc
    14281 ttgacaacat cgccatcagt attgcagaat tagtccgact ctaagaggtg aacattatgt
    14341 cttccctaat tcttagtgtc tctcccgaaa acgtggagag accctactat tttgatatga
    14401 ccagggtcag ggtttattcc attgaagaac tgtattatca ctgttatcat tactggaagc
    14461 agtccattga tgattttatc atgggaaaac tcgaagactg gataaggatt gaacttggtc
    14521 tttcccatat cagcggtcag attgcaaaaa tcaaagacga gtattcttcc attacggagc
    14581 aatatataag atttttaggt ctcatcgatt atttttatga ttcggaatta gaaagtctga
    14641 gaaaagaaat atataaatgg gaacataagg ctgaatggga aaagtgcaag gaaaaagccg
    14701 attatttcat ggctcaggaa aaacccgaag aggctatgaa atggtatatg aaagccatcg
    14761 tctttgacga aaatccaaaa atctataaca atatgggcgt tgcttccatg catatgggtt
    14821 ggtacaggga tgcagcatac tattttcaaa aagcttatca gaaagagccc aatagcatcc
    14881 ctatcttgtt aaatcttacg gatgtatata tccttcaaaa aaattttaaa gaagcaggaa
    14941 aatacctaag ccaggctgcc cagttagacg attcccaagt catctggtac tactacgggg
    15001 aactttacaa cagtatgggc aatcaaaaaa aggctataga agcctacctt aaagctatca
    15061 gcaaaggtga tcattttgac agctatatta agctggcaag aatctacgtt caggaaaaag
    15121 attttgaaac agcccaggcc tatctggaaa aaataaaaac ttcccaaaga aatgaaattt
    15181 actggatgga atattccaaa ctctacgaag catgggataa aaaagacctg gccttagagg
    15241 ctgtccagaa aagcatcagc aaaaacaaaa actacattta ctcatggata gctttagcaa
    15301 gattccagcg taggaataat tgtttagaag aagcggaaaa agccatcagc gaagccctaa
    15361 gaattgatgc agaaaacgaa gaagcccaaa ttgaagcagc aaaaatacaa aaagaattgg
    15421 gaaaagaaaa agaatacaga cagtccttaa aagaactcat caacaaatgg gttcacaggt
    15481 acagacagga tacgatataa agcgaggtaa aggcctcgct ttattatatt caaaaaaatt
    15541 gttgaattat ttgaaaaatt gtcacatatg atgtaaaatt attatatata aaatttagat
    15601 aattatcaca cctttcttaa gaaaggagag cgttatggga tctatagcca atactattaa
    15661 attgtattta atattgcaaa gcaaaggtaa agtaaaaatt aaagaattag ctgaagaact
    15721 tgaagtcgat gaaagaacca taagacgata taaagatgaa ttagaacaag ccaatattta
    15781 tattgattca gaaccaggaa gatatggagg atattatttg tataatgaca attatcttat
    15841 gggtttaaca ctgagtcagg aagaatatct ggccctgatg atgattaatg aagagttatc
    15901 caggagtaat catgttgttt caagggatta taacagtgcc attgaaaaaa ttagtgtagt
    15961 ttacaatgtt catcatcata atgcaacagg aacaaatttt tcaaattata cagtgaaaaa
    16021 atcaaaatcc aatgtaaata tatctgaaga acgtaaaaaa ttaatagata ttcatgcggc
    16081 agccttgact agaaataaag ttaagattaa atattattct ttaagttcag gtttaagtga
    16141 aaggatagtt catccttatg caacaataca atataaagga gatttatatt ttgctggtta
    16201 ttgtgaaaaa agacaagaga tacgtgattt taaactgtgc agaataaggg aatatactgt
    16261 tttggacagc aagtttccta aaaatgataa ttttaattta gaagagtata tgaaaaattg
    16321 tataggcatt attaaagatg aaaaaattga tttaaaaata cagatttttt tcccaatggc
    16381 tcaaatcgta aaagagaaga tatttgtaga taatcaaaaa ataactgaat acaaagatga
    16441 aggttatatc atttacgaag caacaatgga aggtttagaa gaaataaaaa gttggatttt
    16501 aagtatggga agttatgcaa aagttcttga accaaaattt ttgcaagatg aaattaaaga
    16561 tgaaattaaa aaaattataa atatttatta acctgcctat atatgtccgc atatatagat
    16621 ataattaaat tagtgaaata aaggaacaga agggagatgt agtattgcga ttaaagtttg
    16681 aatttatcgt gacaaaaaat aaaatacctc tagattacag gcgtatattt atttcattat
    16741 ttaagaaagc actggaacaa tacaacacgg attccaagaa aaagtattat catgaaaatg
    16801 acccaataaa aaaagcgttt accttttctg tctttctgga tagacctaaa ttttacaaag
    16861 attatataga actgaaacaa aataaaatag atttatattt tagtacatat tcatacgaaa
    16921 ttggaattga ttattataac gcattaatta aaatgctcaa ttatgcatat ccccttcagg
    16981 acgacaatta tttaatctta aaaaatgtag catcgatcaa agaagcaaat atcaaacaag
    17041 actcgattct aataaaaacc atgtcaccaa ttttagctag aaagcatgat aaaaacagaa
    17101 attatgacac atatctactt ccctcccagg aaggtttcat tgaagaaatg cgaaataata
    17161 tttccgtggc attagaaagt ctggacttaa atgatctaaa agaagatgtt caaagttttg
    17221 aacttattga tataggtaag gggaaggaag taaaagtact gttttatggt cataaagttt
    17281 taggcttaat aggcaattat ttcttaaaag gcactccaag attactggaa tttgttttaa
    17341 agtctggaat tggatcaagg acaagcagtg gatttggaat gtttgatgta gagggggtga
    17401 gtaggtgaat gaaataacga ttcaactagg ggactggcta tttaatgctg gtgttgtagg
    17461 acttgttaat atcttacttc atgctgggga tgaagttaag ctgaatggac aagaagtttc
    17521 ttttaacgcc ggggtattgg acaaatttga agaaaaatat tttaagtatt ttattgatac
    17581 ctatgagaaa acactagctt ggtataaggt tgtgagtttt gaagatttta ttaagaaaca
    17641 tgaagaagaa aattttgaga gttttactga aaaagattta gaaattttaa atcaatatat
    17701 aggctcaggt tcaaaaagtg ggactttaaa gtatatatta acgagtaaca gtcacaaagc
    17761 tgcttataat cttattactt ctgaattaga tgtattagaa cttgaaaaac aaatatcaac
    17821 tattaaatta aagaaaaatg aagaattaaa agatcagatc ccaaaaataa aagatttaat
    17881 gaaaaaaatt catgatatta ttagatatta taatcaacct gatgccaaaa agtatattgc
    17941 agcaaagaat gtaatatata cgataattaa taatgcatgg aataatgttt cttttttgaa
    18001 tcgtcaaact aaaataccag atatgtatga ggattataaa atatactttt tgcaaggttt
    18061 aaatgattat ttaaataata ataaaaaaga atataattac agctgttcta attgtggaag
    18121 tttgatgaat gatatgaata atgacattgc ttttttaaat gctacagggt ttgatacaag
    18181 cagaaaatta tcaaatgtat ggaactttaa taatgatatt gccatttgtc ccgtatgtaa
    18241 aatcgtttat tcatgtgtac ctgctggatt tacatatgta tatgacaagg gcatcttcat
    18301 taatcaaaac caggatattc aaagtgctgt taatgttaat caaaggataa agagcgaaat
    18361 tattgacagc ggtgatgtta aaagtatttg ggaattttat agaattgttt ctgaagagat
    18421 ggaaagaaaa attaatgaag gtgtaaagta tgaacttgca gacattcaag ttgttagata
    18481 tgagaatgaa agttacagat tcaatttatt atcaaaaaag atgcttaaag tgctcagtaa
    18541 atcaactaaa gaacttaatg ccttattgaa attaggttat aaagaagtaa atacatactt
    18601 taatatttat gatgaagtaa tgaagagatt acttaataat gaaaatttgt ttttacttat
    18661 acacaaatta atgattcaga aacttacaaa gccttctgaa tcatattatc agatgagaaa
    18721 tattaattat ttacttgaaa ttaataataa ttacttagaa ggagttgttg gaatggaagt
    18781 taaagaaagg gatattttag agctttcaaa aaatgcagga tattatttta ggaaagcata
    18841 tgaagagaga aatgttgaaa ataaaatcaa tggtattgct tatagattat tgaatgcttt
    18901 aaaaaccaat aatcagcata tgtttatgga taccctgatt aattgttatc tttatatcaa
    18961 gcaaagtgtt cctaaagtat ttattgacgg aatgaaagat gactatgagt ttaaaagtgt
    19021 aggctatgct tttgttgcgg gaatgactag tgaatcaaaa aagaatgaag aaaataatta
    19081 aaatggaggg ataattatat gagtaattca actttaaaac ccaaaggatt aactatttca
    19141 atgatatttg aagctgagag tgcaaactat ggtgaaggaa taggtaatgt agcatcttta
    19201 aaaaaggtgg caaggggaaa tggagaacaa tatacatata tttccagaca agcgatcaga
    19261 tataacatca ttgaacaatt aggagaagaa ttggcaaaag ttcaggcaga aggcagtgga
    19321 gataaaaaag ttattcaatt tgcaccagat agcactatta aagattatcc tgaaatagac
    19381 ttttttggat atatgaaaac aaaaaaaggt gagggtagtg tgacccgttc agcaaaagtg
    19441 agattatcca atgcagtttc tttagaaaca tttaagggag atttagattt tcttactaat
    19501 aaaggtttag cggaccgatt aaatgaaaat atgaatttag ctcagtcaga aattcataaa
    19561 acatattatc gatacactat aacaattgat cttgatcaaa taggtataga tgcacaagat
    19621 aatattgaac ttgataatca agaaaaggcc aggagagtta ataagttatt agataccata
    19681 tcaatgttat atagagatat taggggtaga agagaagact taaaacctta ttttgcaatt
    19741 ggtggagttt atgatattaa aaatcctgta tttcaaaatg ctttagaagt tagaaataac
    19801 agagttcaaa tttcacttat tgaaggcgta atgtacgatt ttattaagaa tgatacatta
    19861 tgtggcctag ttgaaggaaa gttcttaaac gacagtgaga ttaaagaaag attaaatgct
    19921 attcctatgc cagaattctt ttcaaaaata aaagaaaagg ttagtgaata ttatgaaagc
    19981 tgttaggtta aagttaagtc aggaacttgt taattataaa gttccaacca gttttcaatt
    20041 aaaagagact tatcctctac ctccctactc tacggttata ggtatggttc acaatatgtg
    20101 taagtatact gaatataaag aaatgaaaat aagtatacaa gggaaatatc tttctaaagt
    20161 aaatgattta tatacgagat atgaatttaa accagaatgt ccatttgaaa aaggaaggca
    20221 tcaattaata attaatggat ttgggataac aaagggtatt gctactgcag aattactcac
    20281 agaagtagaa ttgctaattc atgtaatacc tgaagatcaa agtattatta gagaaattga
    20341 atccgcattt ttaatgcctt gggagtatcc ttccttaggc agaagggaag atctggttgt
    20401 tattgatgaa gtcaaagttg ttgaactgac tgaaaaaatg tttgaaaaga gtatgagtag
    20461 taataaagac tattcagcat atgttcccat agatctgatg gataatataa aatttgggaa
    20521 tcgtcagcaa ggaatcgaat ttgtgggaac aagatacaaa cttaataaaa attattctct
    20581 tcaaacgatt ggaaaaggaa aaagtaaaaa agaatttaga agatggaata aaataaatgt
    20641 tgtctatggc tcaaatattt acggaataat gaaaaaaacg cttttgattg acgaagataa
    20701 ttattttgta tttccagcct aaggaagagg tgattaatat taataattat tgggcaaagt
    20761 ctaatcctaa agaaacaata caggaacata cagattcatt attaaaaaat ttatgtttat
    20821 ttaaaagtat ttatcccaat attccagtaa attacgaatt gcttaagctg gcatgtatct
    20881 atcatgattt aggaaaaatg aatataagat tccaagcaaa aattcaggct agtatcgatg
    20941 gcaaaaaatc ttataaagac aaacttgaaa ttcctcacgg aattttaagt ttgtctttca
    21001 ttaactacaa gtatttagaa gataagggct ttactgaaga cgaaataaga attttatttc
    21061 atgctgtagc ttaccatcat gacagagacc tggattttga agaagaggat ttaggaagag
    21121 aaataagttt attaaaaaaa gaatgtgtag gctttaaata tgataaggcg gatgaattaa
    21181 ttcagttacc agatgaaata gaagcaaaat tttatatgtt aaatgatcga atatatgaaa
    21241 aaaatgatga cgataataat aatcagacat tttatgatta cataaaaatt aaaggtcttt
    21301 tgaatcgatt agattatgcg gcgagtgcag gcataaacgt agaagtagca aatgattttt
    21361 taattcaggg attagaaaag ctgaaagaga aagaaaattg gggagaatgg aataaactac
    21421 aacaatacat gattaacaac cgggataaca atgtagtggt tatagcacaa acaggaatgg
    21481 gaaagacaga ggctggcctt ttatggatag gcaataataa aggatttttt acgttacctt
    21541 taaaaacagc tattaatgaa atatataatc gtattactaa aaaaatagta ccagacagag
    21601 aaaaggtagg cctgcttcat tcagaaacta tttttaagta tcatgaactg caggaaagta
    21661 tagaagaact ggatatagat gagtactatc tcaaaacaag acaactttca ttaccaatta
    21721 cggtttgtac cttagaccaa ttatttgata ttgtttttaa atatcggggt tttgaggcta
    21781 aattagctac cctaagttat tctaaaatcg taattgatga agtccaaatg tattcaccag
    21841 atttattggc ttacttaatt gtaggccttc attatatcat aaaattagga ggcaaatttg
    21901 caatattaac cgctactctt ccaggaattg taaaatatta tttaaagggt gaaggcatcg
    21961 agtttcagga accccagagt ccatttatca aagatgaatt aataagacat agtgtgaagg
    22021 ttataccctc ccaaataaat gcagaattta ttctccagca ctttaataat aataaagtct
    22081 tagtcatttg taatacagtt aaaaaagcaa gagagatttt tgaggattta aataataaca
    22141 aaaaattatt tgataaggat gttacaataa agcttttaca tagcagattt accaggaaag
    22201 acagaagaga aaaggaagag gaaatagtag aatttggatc aaaaaatagt atggaaacag
    22261 gcatttgggt ggcgacccag gtagttgaag cttctttaga tattgatttt gatattttaa
    22321 ttactgagtt atcagattta aatggattat ttcaaagaat gggaagatgt tatcgacata
    22381 gaacatatga taaacaaggg tataattgtt ttatttttga tggtggagaa agaacttgca
    22441 gtggggttgg cagcgtaata gatgaagata tttttgagct ttcaaaagaa gcaattaacg
    22501 atattgatgg aattatgaaa gagtcagaaa aaatgcagtt ggttgaaaaa gtgtattctg
    22561 ctgagaaatt aatgggaaca aagtattatc aggaagttca gaaaactatt aactatatca
    22621 aaacgataac agaatttgaa aaaagtaagg atgatattaa ctttagaaat attgataaca
    22681 ttacaatatt accaaaatgt gtttacttga ataataaagc agaaattgat gaattaataa
    22741 atatttataa ggacgaaaaa acgacaaaag atgataagcg aaaatgttgg atagatattc
    22801 aagattacct tgtgaatgtt cccggttatg aagt
//
LOCUS       DAOSUD010000032        22701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1483_length_22701_cov_25.560585, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000032 DAOSUD010000000
VERSION     DAOSUD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 22701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22701
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_22701_cov_25.560585"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..1491
                     /locus_tag="PLG49_05665"
     CDS             535..1491
                     /locus_tag="PLG49_05665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOQ16845.1"
                     /translation="MDISRKVIKADWAHDRGYSGRGVGVAVLDSGIYPHKDFTIPRNR
                     IIAFKDFINHYDKPYDDNGHGTHVAGIIGGNGISSDGRYMGIAPECYLIGIKILDHKG
                     NGSTSNVLAGIQWMIDNKDRYNIKIANLSIGSPDREGENDPLVKAVNAAWDAGIITLV
                     AAGNDGPRSKSITSPGISRKVITVGASDDQKSVDIHGDIVSNYSGRGPTRACIKKPDV
                     VAPGSDIMSCASDKNYIPDKELSREELKTYYVKKSGTSMATPMVSGALALFLEKNPHI
                     QPNDLKLYLKDCTNDLNFPQDQQGWGLIDIQKLLEGRGRNVR"
     gene            1481..1831
                     /locus_tag="PLG49_05670"
     CDS             1481..1831
                     /locus_tag="PLG49_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16846.1"
                     /translation="MSDENMNDVTGMLMNKEYIRKDIDEMTQLLKNMLKEKYISKMND
                     FVMKHKERTIKNPTKEVVLMEAIKPFLQKEDRLNLDRMIEMMNLINTARSIQMEVQRF
                     RTQMMDDHVKMEKE"
     gene            complement(1891..2046)
                     /locus_tag="PLG49_05675"
     CDS             complement(1891..2046)
                     /locus_tag="PLG49_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16847.1"
                     /translation="MSSAKGFSGGYSIIFLFFFLLLFNEGYGSKFDNSLFFFLLIFLI
                     LFGGIGY"
     gene            complement(2177..2380)
                     /locus_tag="PLG49_05680"
     CDS             complement(2177..2380)
                     /locus_tag="PLG49_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16848.1"
                     /translation="MNHLNQNSYELQDIPEPFRGQHKDFSINPNPGQIDSQPVTSSGF
                     YPSFKIEDLLLILLLFLLLNDNK"
     gene            2776..3018
                     /locus_tag="PLG49_05685"
     CDS             2776..3018
                     /locus_tag="PLG49_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16849.1"
                     /translation="MDFVCPLCNGFALIELNCEKCNKKMVDKGREADYYDSYSPYLPM
                     SLTSQIDGYPEDKCVHIFYCEHCGKSSRFAVERKRI"
     gene            3342..4730
                     /locus_tag="PLG49_05690"
     CDS             3342..4730
                     /locus_tag="PLG49_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOQ16850.1"
                     /translation="MIEFNKKTNLLERTEFVYPLQDVTEPNLYRNIFNYEEIPKVVFN
                     HRHVPINMPKEIWITDTTFRDGQQSREPYTTEQIVHIYDLLHKLSGPKGIIRQSEFFL
                     YSKKDRDAVYKCLERGYQYPEVTSWIRASKKDFEMVKEIGLKETGILVSCSDYHIFYK
                     LKMTRKQAVDHYLSVVRECIETGVRPRCHLEDITRADFYGFVVPFVYELMKLSEESGV
                     PVKIRACDTMGYGVTYPGATLPRSVPGIIYGLVHHARVPSELLEWHGHNDFYRAVNNA
                     STAWLYGASAVNCSLLGIGERTGNCPLEAMVIEYAQFRGTTDGMDTTVITEIAEYFEK
                     ELNYQIPPMTPFVGKNFNVTRAGIHADGLLKNEEIYNIFDTDKLLNRPCRVAISNTSG
                     LAGVAHWINSYFNLKGDKVLDKNNEIVVKVKEWVDAQYDEGRVTVITDEELEEVTLAI
                     MKELNYVLEGEL"
     gene            4732..6660
                     /locus_tag="PLG49_05695"
     CDS             4732..6660
                     /locus_tag="PLG49_05695"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOQ16851.1"
                     /translation="MGLNLTQKILKEHLVEGKMIPGEEIGIRIDQTLTQDSTGTMAYL
                     QFQAMGIDKVKTKRSVAYIDHNMLQQGFENADDHKYIQTVAHKHGIYFSRPGNGICHQ
                     VHLERFGVPGETLLGSDSHTPTAGGLGMIAIGAGGLDVAVAMGGGTYYITMPKVVNVR
                     LTGKLQPFVTAKDIILELLRRLTVKGGVGKVIEYTGDGVKSLSVPERATITNMGAELG
                     ATTSIFPSDEVTRAFLKAQKREEVWKELLPDEDAEYDEVIEINLDELEPLTAAPHSPD
                     NVKKVSELKDVKVDQVAIGSCTNSSYMDLMKVAKILKGKTVHPSVSLVIAPGSKQVLT
                     MLAQNGALADLIAAGARILESGCGPCIGMGQAPASNAISLRTFNRNFKGRCGTASASV
                     YLVSPETAAISAITGKLTDPRTIGEEISVEMPEEFIVDDNMIIPPADDPDSVEVIQGP
                     NIKPFPINTPLKDEVTGKVLIKVEDNITTDHIMPSNAKLLPFRSNIPYLAEFCLTPCN
                     EEFPKRAKENGGGFIVGGENYGQGSSREHAALVPLYLGVKGVLAKSFARIHKANLINS
                     GILPLTFKNPEDYDKIQEFDELVIENAVEQVKGDTVVVKNKTQGTQYEMSLEVSERER
                     QMLLAGGKINLIKQQNAK"
     gene            6683..7849
                     /locus_tag="PLG49_05700"
     CDS             6683..7849
                     /locus_tag="PLG49_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOQ16852.1"
                     /translation="MDYTKMIEEAKEKFGKLVEEQLKRVEMMKSQGDFLDYKSLDQII
                     IGIVGGDGIGPAITAQAQRILEFLLKDEVEKGKVKFKVIDGLTIENRAKVNKAIPDDV
                     LEEIKKCHVILKGPTTTPRAGDPWPNIESANVAMRKELDLFANVRPVRVPELGIDWTF
                     FRENTEGAYALGSKGFHITDDLAFDFKVMTTQGIERIARAAFDYARANGKTKVTVVTK
                     ANVVKTTDGKFLNICKEVAKDYPEIEMDDWYIDIMTAKLVDEKRRRDFQVFVLPNLYG
                     DILTDEAAEFQGGVGTAGSANIGKRYAMFEAIHGSAPRMVAEGRDKYADPSSIIRAAS
                     MLLSHIGYVEESKKLDQAMDICTRTEKKLVITGRDTGATNQEFADYVMDTIKSL"
     gene            7920..8603
                     /locus_tag="PLG49_05705"
     CDS             7920..8603
                     /locus_tag="PLG49_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ16853.1"
                     /translation="MVSYDLSKEVSDKYSLQGRVFHKIREDILNGKYKTGDSIKEIVV
                     SKELGVSRTPVREALKQLELEGLVISIPNKGAVVAGITEQDIMDIYIIRSLIEGLAAR
                     WAAAHITDEQLKRLEEIIELTEFYAGKHDVEHLHELDTQFHEIIYEASNSRPLKHILS
                     DFHIYVQRARMESIAMPGRDLKSTQEHKAILKALKDRNPEEAERLTNQHVRNVTENMM
                     KHLKKADRI"
     gene            8854..9351
                     /locus_tag="PLG49_05710"
     CDS             8854..9351
                     /locus_tag="PLG49_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16854.1"
                     /translation="MNIRKAVLEDVPVLVKLRMDFLTSYVKPMTEEESRIIEEQLFIY
                     FQKHIPLGDFIAYLCEINDKPVSVAFLTISEKPPNPNFITGKTAMIHNVFTYPEYCRR
                     GIASKLLQLLIEEARQQKVSSVELLATEAGKPLYRKLGFTEAENTFMRLKVLDKDLLL
                     MAELA"
     gene            9381..9900
                     /locus_tag="PLG49_05715"
                     /pseudo
     CDS             9381..9900
                     /locus_tag="PLG49_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323429.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
     gene            complement(9995..11902)
                     /locus_tag="PLG49_05720"
     CDS             complement(9995..11902)
                     /locus_tag="PLG49_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ16855.1"
                     /translation="MILSCKDISKSFGIDQILKDISFQIEEKEKVAVTGVNGAGKSTL
                     FKILAGEIAPDSGEIFKAKEAVIGYLPQNAEMDSEHTIYEEMLKVFKDVMELEANLHT
                     LEKEMSTLNGSKLTKLMDKYSALQHQFEELNGYGYKSHIKGVLKGLGFNDNQLCQPIN
                     QLSGGQKTRVALAKLLLSSPSLLLLDEPTNHLDISAIEWLEEFLKNYPYAVLIISHDR
                     YFLDKLVTKVIEIENNQASLYKGNYSYYAKQKQINREIQLKQYINQQKEIKRQEEIIQ
                     TLRSFNREKSLKRAISREKALEKMEKIQAPAALPASMKFSLKPRFESGNDVLHVENLS
                     KSFNGKKLFQNISFHLKKGEKVALIGPNGIGKTTLFRILLNQLNYDEGSFRLGTNVKI
                     SYYDQEQQNLSLNKTIIDEISDAYPSLTLGEIRNILAAFLFTGDDVFKPIASLSGGER
                     GRVSLCKIMLSEGNFLLLDEPTNHLDLISKEILEDAINHYTGTILYISHDRYFINKTA
                     SKILELSPYGITEYLGNYDYYLEKRAQIKDDVKASMEKEEKTKSSKDEWLRQKEEQAQ
                     QRKLQAQIEHIENQIHDIERQIEEINELLCLEEVYTSPEKSKEAYDAKVSLEEKLESL
                     YQQWEELHSNL"
     gene            12032..12700
                     /locus_tag="PLG49_05725"
     CDS             12032..12700
                     /locus_tag="PLG49_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOQ16856.1"
                     /translation="MPANRRISMAVIRRLPRYYRYLGELLENGIKRISSKELSERMGV
                     TASQIRQDLNNFGGFGQQGYGYNVEYLYKEIAKILGLDNKYKVIIVGVGNLGQALANY
                     VKFEERGFVLCGLFDVNPRLIGMTIRGIEVKDIDEMEAFVKENKIDIAYLTLPKSKVR
                     KVAEDLASWGVKGLWNFSPVDLDLSSDVVVEDVHLSDSLMTLSYRINRQMQKKEAQSK
                     KLHK"
     gene            12719..14218
                     /locus_tag="PLG49_05730"
     CDS             12719..14218
                     /locus_tag="PLG49_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOQ16857.1"
                     /translation="MEYAKFFISPVIGGIIGYFTNWLAIKMLFRPHKEIKVFNLKLPF
                     TPGLIPKEKERIARSVGTTTAKYLLSEEVIVRALSSEELLESIAHVAADTYHDICYKN
                     NYSLNDFLTFILEDDKEKNIELISDKLTSWVIDYLSQEENLDNFASFIASFIEDKLDV
                     CIGDIKLEDYIKIDFLIEESVKHQLIHQILHLKNQLCKDNFSSSRIIPDIFIEMAMDF
                     AKSQLSEWMPLALGILEQPSVENKIKEILQEFIRNNLGKLTLIFVDVDKLYNKIKSYL
                     KSCIEDETNQKAIFEQIDMFIKQLLEKPVCQWIEKIEFLKNEKEFEKTYDKIIFYLSQ
                     GEKTETLKEKLKEYINNQKEKNILSIVKSVKPDIDQAIENRIKSYLKHLIAREEWKEF
                     IRNLIKKESDRLLSTPVNQWFEGLESVVEDKIKHFTLKAYKAFVLKHSSDFIKQLDIS
                     DIVENRIIEFDTDEMEEIILSVVDRELKAITWLGGLLGLVIGFVPLLLS"
     gene            14360..16063
                     /locus_tag="PLG49_05735"
     CDS             14360..16063
                     /locus_tag="PLG49_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOQ16858.1"
                     /translation="MKKLFSGVFFFIFFFLLFSSNGLAKAREEYGLPLASYKGEKGVT
                     IVSFSYNWASQEKLKEVYDELLKNFHGEEIKYLTYIYIYPDSPEGVLACYYEDYDINS
                     EGEYVYKPGRYIEIFNGDQYTDVSQFARVLAHEYGHHFTLYYLMTKENKHFNQWKETN
                     YAKIRGLEKYKEVGYFSVQDNRYSYQWDVAEIAANDYVQLFGSPNAKKSTDYKDVQER
                     VEQNIKASYYSTDSFNLSPQENLSIPLAADVPGLYLYWLGLAGYTSMEPDIPVKPQIS
                     IKKHKEIMSGYFQYEVTWNPISKYDEYEYTLVAYPAADYIIFPRPIKTVSPGESTKAY
                     FGSAMHQNENGTVNLILDDQYEGEFYFRLFIKDKRGFIFSTEPVKYDFNKDKVKSIYR
                     MTDIPPNHWAIHYIETIIDKKIAEGYEDGTFRPEGEITKVEFMAMLMRAIHYERMPEE
                     DGRDWFEKEGYLEAARQLNLVNISDCRANYNQLNDPITREEMAFMAGQVLKYLGYRYE
                     KGYQAEFKDIGQVKYKDEISLAVYYYIINGYPDGSFKPERHVTRAEASKVIYKLLDFV
                     D"
     gene            16195..16659
                     /locus_tag="PLG49_05740"
     CDS             16195..16659
                     /locus_tag="PLG49_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16859.1"
                     /translation="MMNIRKAQLKDIDHLIFLWNQLINYHKDKKLYFPLSRDWKEEKR
                     RELEWVIKGALSVIYIIEEDKPIGYIRGTIHKLPLLYDIKYEGSIEEIFILSPYRRKG
                     YGKKLTEALIQDFKKKEVEYININVDIDNEIGQVFWDALGFKTVSLKKSFKL"
     gene            16670..17275
                     /locus_tag="PLG49_05745"
     CDS             16670..17275
                     /locus_tag="PLG49_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16860.1"
                     /translation="MLFWKKDIKLDDGLLKKVPILILDKNWHNIFPQEKKTKKILLLE
                     ETLSNLLKEQGQLNNDYKDYIKLKKQLMGDILNLTDEAFTNEIEMAKEKMGKNKKYIL
                     EINNKLKKIQKRLEKLPDEIEETNYRLLKESVIISYKEMKVHQKKLKELTEWIEKTRE
                     ILKNKIEERTIHEEKASEIYSYLHDLVGLELIEYLDKKLWR"
     gene            17280..17651
                     /gene="acpS"
                     /locus_tag="PLG49_05750"
     CDS             17280..17651
                     /gene="acpS"
                     /locus_tag="PLG49_05750"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOQ16861.1"
                     /translation="MVIGIGTDIIEIKRIEEAVKKNSNFMKKCFTAREQELFESKKNN
                     LHTIAGTFAAKEAVSKALGTGFAKFELKDIEIVRDEKGKPCVLLHNKAKEIMEQLMIS
                     EILLTISHSREYAIAYALAQN"
     gene            17694..19268
                     /locus_tag="PLG49_05755"
     CDS             17694..19268
                     /locus_tag="PLG49_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOQ16862.1"
                     /translation="MKICSGEEMRMIDEATINKVGIPGIILMENAVIAVVNEIIKDIR
                     PTVNTRVTVFCGQGNNGGDGFGIARHLSHMDLNVTVVFIGDYNCLRSDAKTNFDILQN
                     LRIPIKTISPASVVDDEIAYLIQRSDIVVDAIFGTGLSKKIEGLYKDLIDLINFYGKY
                     IISVDIPSGIDSRTGAVLGNAVKADKTVTLALPKAGLYLYPGTDYVGKLVIADIGIPR
                     RIIEEAELKMNVLTDEEVKSLIPIRFTRSNKGSYGKVQVIAGSKGMTGAAALTCLSAY
                     KVGAGIVSLGVPASINNILEEKLTEVITVPLPDNEGKLCKESFYAIKAHLENASMIAI
                     GPGLGQNAEIIELMELIIQNSKVPIVIDADGINAVARNIEMLKNIKVPIVLTPHPGEM
                     ARLTGQTIEEILEKPVEIVRDFCHKWNVILVLKDAKTIIGHPNGEIYINTTGNPGMSV
                     AGSGDVLTGIISGLIAQNMDPYKAAVLGVFLHGKAGDLAAKELGYHGMLAGDICRYTP
                     EAIKMYYENQEVRNEG"
     gene            19258..19704
                     /locus_tag="PLG49_05760"
     CDS             19258..19704
                     /locus_tag="PLG49_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ16863.1"
                     /translation="MKAKDIMERNVLAIKKDVFVKDIAKIMFENKISGLPVVDDENKV
                     IGVVTEKDLFLKEKESTFPAYIEFLGSLLFSEEIKKYDEELKKLSQTTAEEIMTREVH
                     TITEDATIEEIANIMVNKNVNRVPVVDKGGRIVGIVSRADMLKTII"
     gene            19723..20370
                     /locus_tag="PLG49_05765"
     CDS             19723..20370
                     /locus_tag="PLG49_05765"
                     /inference="COORDINATES: protein motif:HMM:NF041287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS-related protein"
                     /protein_id="HOQ16864.1"
                     /translation="MTGAKKLLLLFILATGCFLTSCSFKEKSKSPIEKIHEKLTSMEN
                     YGCVADVRFISNKGENTYKTKQHYKMTGEYRLEILAPENLQGLVTVYDGQKLIQYNPR
                     ILGEVITEQPESESANEIFLGAFLKNYLQSEGVALEVFNHNSENYTVLEAVIPDGGKY
                     LATEKLWISNKSLNPVKLVIYDKEGKERIIVEYLEFKYNVNLDESVFRINKSNSV"
     gene            20400..21596
                     /gene="alr"
                     /locus_tag="PLG49_05770"
     CDS             20400..21596
                     /gene="alr"
                     /locus_tag="PLG49_05770"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOQ16865.1"
                     /translation="MTSEHYRVIAEINLDAISNNIKEIQKNISKDTEIMAVVKADAYG
                     HGAIEVSRILIENGVQRLAVAILDEGKQLREEGFNVPILILGYTPAEQAGELVSYDLI
                     QTVFSFEMAKAISDSAVKQNKKAKIHLKIDTGMSRIGFKPDKESIKTIKEINQLPNIN
                     IEGIFTHFSSADEADTSFTQQQFSIFNAFIKALEEEGLKIPVKHCSNSAGFMLDEKMH
                     MNLIRPGIIIYGLYPSNEVNKEKIHLKPAMSLKSQVVFVKEVEENTPVSYGRKFITSK
                     KTKVATIPVGYADGYSRRLSSSGRVLINGEYAPIIGNICMDQFMVDVSHISDVEVGTE
                     VVLIGQQGQNSISAEEIADIIGTINYEVVCMVGKRIPRLYIENKEVIKKIMYVNESEK
                     IDEKIN"
     gene            21747..21932
                     /locus_tag="PLG49_05775"
     CDS             21747..21932
                     /locus_tag="PLG49_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16866.1"
                     /translation="MSKKNKIFKIKKTSDEEQDIMIKGYQEMAEINLDLSKLCFEAER
                     EVEKIIEQVVECERCGG"
     gene            21922..22263
                     /locus_tag="PLG49_05780"
     CDS             21922..22263
                     /locus_tag="PLG49_05780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOQ16867.1"
                     /translation="MVVKRGDVFYADLSPVVGSEQGGVRPVLIVQNDIGNKYSPTVIC
                     AAITSQINKAKLPTHIEIDAKSYALVKDSVVLLEQIRTIDKRRLKEKICHLDKELMKK
                     VDKSLMISFGL"
     gene            22453..>22701
                     /locus_tag="PLG49_05785"
     CDS             22453..>22701
                     /locus_tag="PLG49_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOQ16868.1"
                     /translation="MIIDIHTHCFSDDIARKAVPLLAQKANVLPYTDGTIADLKRSMK
                     ENGVDVSVIQPIATKAKQTPVINSWIMTIEDSEIIPFGT"
BASE COUNT         8100 a         3087 c         4556 g         6958 t
ORIGIN      
        1 ccgcagtatg atttagaatc agccataaag atgcctcctt atttataaat aatatcttac
       61 gtttatattc tatgcataag ttacaggtta atgacaaaag gatgaaaaaa atgaagaatt
      121 ttgggacaac tccttgttat ggagaagata aaaaagtgta aagcattaca aagtctttaa
      181 tagattaatt atttgattca tacactagta tagagttatt gtaaataaga aaggagtaag
      241 aatgtgaagc ggtcattggc attattgtta ttggggataa tgagccagac aaataaaaag
      301 accatatata aagatgaaag tgaaaagaca aatcttaagg aagatacagt acagcttatt
      361 gttgaatgca gttcagagga ggaagaatgc cagatatgta aactgggtaa ggtaaaatac
      421 aggcttccta tgattcaatc ttatgttgta gaagttccta aaaataatat tcacaagtta
      481 caggctttaa aaggcgtaag aagagttcag gaagattccc acattactgc ccagatggat
      541 atttcaagaa aggtgataaa agcagattgg gctcatgacc ggggctacag tggaagaggg
      601 gtaggggttg cagtattaga ttcagggata tatccccata aagatttcac aatccccaga
      661 aatagaatta ttgctttcaa ggattttatt aatcattacg acaaacctta tgatgataat
      721 ggtcatggca ctcatgtggc aggaattatc ggaggaaatg gtatatcttc tgacggcaga
      781 tatatgggga tagcaccgga atgttattta atagggatta agattttaga tcataaaggg
      841 aatgggagta catccaatgt tttggcaggc atacagtgga tgattgataa taaagacagg
      901 tataatatca agattgctaa tttgtctata ggcagtcctg acagagaagg agaaaatgat
      961 ccattggtaa aggctgttaa tgcagcctgg gatgcaggta ttattacttt ggttgcggca
     1021 gggaatgatg gaccaagaag taaaagcatt acgtctcctg gcattagccg gaaggttatt
     1081 acggtaggcg cttctgacga tcaaaaatct gtagacattc atggtgatat tgtatccaat
     1141 tattcgggta gaggacctac cagggcttgt ataaaaaaac cagatgttgt tgcaccgggt
     1201 tctgacatta tgtcttgtgc ttcagataag aattatattc ctgataaaga actatccaga
     1261 gaagagctga aaacttatta tgtcaaaaag agtgggactt ctatggcaac ccctatggta
     1321 tcgggggcac tagcactttt tcttgaaaag aatccccata ttcaacccaa tgatttaaag
     1381 ttgtatttaa aagattgtac caatgacctc aactttccac aggaccaaca aggctggggt
     1441 ttgatagata ttcaaaagct tctggaaggg aggggaagaa atgtcagatg agaatatgaa
     1501 tgatgtaact ggcatgctaa tgaataaaga atatataaga aaagatatcg atgaaatgac
     1561 tcagctttta aaaaatatgt taaaggaaaa atatatatca aaaatgaacg attttgttat
     1621 gaagcataaa gaaagaacta taaaaaatcc aactaaagag gttgtattaa tggaagccat
     1681 aaagcctttt ttgcaaaaag aagaccgttt gaatcttgac agaatgattg agatgatgaa
     1741 tcttatcaat acagcaagaa gtattcagat ggaagtacaa cgttttagaa cacaaatgat
     1801 ggatgatcat gtaaagatgg aaaaagaata agattaatct tagaaatggc aaaaggtaca
     1861 tatctttttt agatatgtac cttttatggc ttaatagcca attccaccaa ataaaattaa
     1921 gaaaattaat aggaaaaaga ataatgaatt atcaaatttt gagccatatc cttcattaaa
     1981 taataataag aagaagaata aaaatataat actatatcct ccactaaaac ctttagctga
     2041 tgacatacat gggcacctcc tttcagccaa tatttcacaa tggatggttt ttcattggta
     2101 tgttatatac tatgaagaat taaaaataag tgttactaaa gaaaaagtcc ttaaaaggac
     2161 ttttctttag taacacttat ttattatcgt ttagtaatag aaaaagtaat aaaatgagaa
     2221 gcaagtcttc tattttgaaa gatggataaa agccagagct tgtcactggt tgagaatcta
     2281 tttgtcccgg attaggattg atggagaagt ctttatgctg tcctctaaaa ggttcaggta
     2341 tatcttgaag ctcataagaa ttttgattta agtggttcat cttttacatt agcccccttt
     2401 attattttga aacatcttct taattataaa tctatgtaga ataaattatt tatatgacat
     2461 cataataata agtaaaaatt ataagttata attcgattta tgtaacccaa aaaaataaat
     2521 atccacaatg aaaatatgta taaaaattag atgtgaagaa tgtggaaaaa tgaaaaaatc
     2581 cttaaaaagt aatgcgtaga gctgtgcata acgttgtgga ttatgtggat aaacaaaaaa
     2641 gttattcaca taatccataa aatttatgtt aaatggtggt gaaaaattaa aacaattatt
     2701 tactttataa tgggttttgt ttaagagcgg ttaatatttt ttcaactttt aaaagcttta
     2761 gaaaggaatg tttatatgga ttttgtttgt cctttatgta atggttttgc tttaatagag
     2821 ttaaattgtg aaaaatgtaa taaaaaaatg gttgataagg ggagagaagc ggattactat
     2881 gattcctaca gtccttatct tcctatgtct ttaacgtcac aaatagatgg ttatccggaa
     2941 gataaatgtg ttcacatatt ttattgtgaa cactgtggaa aaagtagtag atttgctgtg
     3001 gagaggaaaa gaatataaca ggtttcaaaa ttttcattaa cagatggtct gaatctgaag
     3061 gaattaaaaa gagtaaattc attttctatc taacccatga attttattta aaaactttag
     3121 ttaagcgtaa aaagttatta aaatcatgtt ttttaaatga attaagagac aacttaaata
     3181 aataaacaag ctgcttgtaa ttatatgtta attgaaatgc aacttattat ttttacaata
     3241 caattttaag aatattcatt gacaagataa gtataattgt atacaattat acttattaaa
     3301 ttgttataaa agcttatcag tgtaaaatga gggggaaggc catgattgaa tttaataaga
     3361 aaaccaattt attagaaaga acagaatttg tatatcctct tcaagatgtg acagaaccca
     3421 atttatacag gaatattttt aattacgaag aaatacccaa agtcgttttt aaccatcggc
     3481 atgttccgat caatatgcct aaagaaatat ggattaccga tacgactttc cgggatggac
     3541 agcaatccag ggaaccttat acaactgaac agattgtgca tatttatgat ttgctccata
     3601 aactttcagg ccccaaggga attataaggc aaagtgaatt ctttttatac agcaaaaaag
     3661 acagagatgc ggtatataaa tgtttggaaa ggggctatca atatccggaa gtaacaagct
     3721 ggatccgtgc ttctaaaaaa gattttgaaa tggttaagga aattggctta aaagagacgg
     3781 gaatattggt aagttgttca gattatcata ttttttataa attaaaaatg acaaggaaac
     3841 aagcagttga ccattacctg tcggttgtga gagaatgtat tgagaccggt gtcagaccaa
     3901 ggtgtcattt agaagatatt acaagggcag atttttacgg ctttgttgtg ccatttgttt
     3961 atgaacttat gaagttgagt gaggaatcag gggtgcctgt taaaattcgt gcttgtgata
     4021 ccatgggtta tggcgtgaca tatcctggag caaccttacc cagaagtgtt cctggtatta
     4081 tttatggcct ggtacatcat gcaagagttc cttcagaact ccttgaatgg catggtcata
     4141 atgattttta cagagctgtg aataatgcat caactgcttg gctttatggt gcttctgctg
     4201 ttaattgttc cttattgggc ataggagaaa gaacaggaaa ctgtccttta gaagctatgg
     4261 ttatagagta tgcgcaattc agaggcacaa ccgatggaat ggatacgaca gtcattacgg
     4321 aaattgctga gtactttgaa aaagaactca actatcagat tcctcctatg acgccttttg
     4381 taggaaagaa tttcaatgtg accagagcag ggattcatgc tgacggatta ttgaagaatg
     4441 aagaaattta taatatcttt gatactgaca aattactgaa tcgtccttgt cgtgtagcaa
     4501 tatccaatac atctggtctg gcgggggtag cccattggat taacagttac ttcaatttaa
     4561 aaggtgataa ggttctagac aaaaacaatg aaattgtagt aaaagttaaa gaatgggttg
     4621 atgcccaata tgatgaaggc agagttacag ttattacaga tgaagaacta gaagaagtaa
     4681 ctttagcaat catgaaagaa ttaaattatg tattggaagg agaattataa tatgggactc
     4741 aatttaacac aaaaaatact taaggaacat ttagtagaag gaaaaatgat tcccggagaa
     4801 gaaataggaa ttcgtattga tcaaacttta actcaggatt ccactggtac catggcttat
     4861 cttcaatttc aggctatggg tatagacaag gtaaagacta aacgttccgt tgcttatatt
     4921 gaccataata tgcttcaaca aggttttgaa aatgccgatg accataaata tattcaaact
     4981 gttgctcata aacatggaat atatttttcc agaccaggta atggtatttg ccatcaggtt
     5041 catcttgaac gttttggcgt tccaggtgaa acccttttag gttcagatag ccatacacct
     5101 actgcgggtg gccttggtat gatagccatt ggggctggtg gattagatgt agcagttgct
     5161 atgggtggag gaacttatta tattaccatg ccaaaggttg taaacgtaag attaacaggt
     5221 aaattgcaac cttttgtaac agctaaagat attattttag aattattaag aagattaacc
     5281 gttaaaggcg gcgttggtaa agttattgaa tatacaggag atggagtaaa atccttatcc
     5341 gttcctgaaa gagcaaccat aaccaatatg ggagcagaat taggtgccac aacttctatt
     5401 ttcccaagtg atgaagtgac cagagctttc ctgaaagcac aaaaaagaga agaagtatgg
     5461 aaagagttat tacctgatga agatgcagaa tatgatgaag ttattgaaat taatttagat
     5521 gaacttgaac ctttaacagc agcacctcat agtcctgata atgttaagaa agtatctgag
     5581 ttaaaggatg ttaaagttga ccaggtagct ataggaagtt gtaccaacag ctcatatatg
     5641 gacttaatga aagtggctaa aattttaaaa ggtaaaacag ttcatccaag tgtaagcctg
     5701 gttattgctc caggctctaa gcaagtgtta acaatgcttg ctcaaaatgg agctttagca
     5761 gacttaattg ctgcaggtgc aagaatctta gaatctggct gtggtccttg tattggtatg
     5821 ggacaggcac cagctagcaa tgccatttca cttcgtactt ttaatcgaaa ctttaaaggt
     5881 cgttgtggta cggcatcagc aagtgtatat cttgtaagtc ctgaaacagc agcgattagt
     5941 gctataacag gaaaattaac agacccaaga acgattggtg aagagatatc tgtagaaatg
     6001 ccagaagaat ttattgtgga tgataacatg attattcctc ctgcagatga tccagattct
     6061 gtagaagtta ttcagggtcc taatataaag ccatttccta ttaatacgcc attaaaagat
     6121 gaagtaactg gaaaagtatt aattaaagta gaagacaaca ttacgacaga ccatattatg
     6181 ccttcaaacg caaagctttt gccctttaga tccaatattc cgtatttggc tgaattttgt
     6241 ttaacaccat gtaatgaaga atttcccaaa agagcaaaag aaaatggcgg cggctttatt
     6301 gtagggggag aaaactatgg tcagggttct tccagagaac atgctgcttt agttccactg
     6361 tatttaggtg taaaaggggt tcttgcaaaa agttttgcca gaattcataa agcgaattta
     6421 attaattcag gaatactgcc tttaaccttt aagaaccctg aagattatga caaaatacag
     6481 gagtttgatg aattggtgat tgaaaatgct gtagaacaag taaaaggaga tacagtggtt
     6541 gttaaaaata aaactcaggg cactcaatac gaaatgagtt tagaggtttc agaaagagaa
     6601 agacaaatgc ttttagcagg cggtaagatt aatcttatta agcaacaaaa tgcaaagtaa
     6661 gaataataag gaggagcatg acatggacta cacaaaaatg attgaagaag ctaaagaaaa
     6721 gtttggaaag cttgtagaag aacagcttaa aagagtagaa atgatgaaaa gtcagggaga
     6781 ttttcttgat tataaaagtc ttgatcaaat cattattggt attgtagggg gagatggtat
     6841 aggtcctgcg attaccgccc aggctcaaag aattttagaa ttcttattaa aagatgaggt
     6901 agaaaaagga aaggttaagt ttaaggttat tgacggactt actatagaaa acagggcaaa
     6961 agtgaataag gctattcctg acgatgttct ggaagaaatc aaaaaatgtc atgtaatatt
     7021 gaaaggacct acaacaactc caagagccgg agatccatgg cctaacatag aaagtgctaa
     7081 tgtagccatg agaaaagagt tagacctctt tgccaatgtg cgtcctgtga gagtgccgga
     7141 gttaggaatc gactggactt tcttcaggga aaacactgaa ggagcatatg ctcttggttc
     7201 taaagggttc catattactg atgatttagc ttttgatttt aaggtaatga ccactcaagg
     7261 tattgaaagg attgccagag cagcttttga ttatgcaagg gctaacggaa agacaaaagt
     7321 tacggttgtt accaaagcaa atgtagttaa gacaactgac ggaaagtttc tcaatatatg
     7381 caaagaagtg gctaaagatt atccggaaat cgaaatggat gactggtata tagacattat
     7441 gactgctaaa ttagtcgatg aaaaaagaag aagagatttt caggtatttg ttcttccaaa
     7501 tctttatgga gatatcttaa cggacgaggc agctgaattc cagggtggtg taggaacagc
     7561 cggcagtgca aatattggta agcgctatgc tatgtttgaa gcgattcacg gttcagcacc
     7621 cagaatggtg gctgaaggca gagataagta tgctgacccc agtagtatta tccgtgctgc
     7681 ttctatgctt ctgtctcata taggttatgt agaagaatct aagaaattag atcaagccat
     7741 ggatatttgt accagaacag aaaagaaact tgttattaca ggccgtgata caggagcaac
     7801 caatcaggaa tttgctgatt atgtgatgga taccattaaa tctttataat aaatgctata
     7861 cctcctgtga attaaggcag tttttgcttt ataaatcatt ggagtcatta aggaggacga
     7921 ttgtgagcta tgatcttagt aaagaagtga gtgataaata ctcacttcag ggtcgagtat
     7981 ttcacaaaat aagagaagat atcttaaatg gaaaatacaa gacaggagat tcaataaaag
     8041 aaattgttgt ttctaaagaa ttgggtgtaa gccgtactcc agtaagagaa gcattaaaac
     8101 aattggaact tgagggtctt gtaatctcta tccctaataa aggagctgtc gttgcaggga
     8161 taacagagca agatattatg gatatttaca tcattcgttc attaattgaa ggtttggctg
     8221 caagatgggc ggcagctcat ataaccgatg agcaattaaa acgcttagaa gaaatcattg
     8281 aattaacaga gttttatgca ggcaaacacg atgtagaaca tttacatgaa ttagatacac
     8341 aatttcacga aattatttac gaagcttcca atagcaggcc cttaaagcac attttatcgg
     8401 attttcatat ttatgtgcaa cgggccagaa tggaatcgat agccatgcca ggacgggatc
     8461 tgaaatcaac ccaggagcat aaggctatac taaaagcatt aaaagataga aatccagaag
     8521 aagcagaaag acttaccaat caacatgtca gaaatgtaac tgaaaatatg atgaagcatt
     8581 taaagaaagc tgatagaatt taattctatc agctttttta cctattcaga gatctgtttt
     8641 tgaaagcaaa aactctaagg attaaacata taagagatgt ctaaaaatca gttttttagg
     8701 atgaaattca tacattaaaa aataaaaaaa atattgactc ttgcctataa ataaagtatt
     8761 atgtttcata ttatttttaa tttttaccaa aagagtatac gcaatgaatt gttttttggc
     8821 tgaatttaaa attaagtact ttaggggggt tttatgaata tccgaaaagc ggttttagaa
     8881 gatgtacctg tactggtaaa gcttcgaatg gatttcttaa cttcttacgt aaaacctatg
     8941 actgaagagg aaagccgaat tattgaggaa caattattta tttattttca gaagcatatt
     9001 cctttaggcg actttatagc ttatttatgt gaaattaatg ataagcccgt ttcagtggct
     9061 tttttgacaa ttagtgaaaa gccgcccaat cctaatttta ttactgggaa aacagctatg
     9121 atacataatg tttttactta tccggaatat tgcagaaggg gaatcgcttc taaattactg
     9181 caattgctta ttgaagaggc aagacagcaa aaggtttcat ctgttgaact gttagcaact
     9241 gaagcaggca agcccttata tcgcaaatta ggatttacag aagcagaaaa tacctttatg
     9301 agattaaaag ttttagataa agatcttctt ttaatggctg agcttgcatg atttttagag
     9361 gaggatatct tttgacagct ataaatttta ttcttagaaa atggaaagct gaggacgcta
     9421 gctaaaagca ttgcagaata tgcaaataat aaaaagattg ctgataaatt acacaatgca
     9481 tttccttatc catatcgttt aaaagatgca gaagaacata ttaatgcctg tatatcggct
     9541 gatgaatcaa aagtggtttt aagggccatt gacatagatg gcatagcagt tgggagcata
     9601 ggaatttttc taaaagatga tgtttattgc aaaagtgctg agttaggcta ttggcttgga
     9661 gaaccttttt ggggaaaagg aattatgacc caggctgtaa agcaaatttg taaaatggct
     9721 tttgagaaat atgatattgt caggatattt gcagaacctt atgctgataa taccgcttca
     9781 aggcgtgtcc tcgaaaaggc aggttttcgg ctggaaggta ttctgagaaa aagtgtttat
     9841 aaaaaggggc aaattatgga ttcatgtatt tatgccctta taaaagatga ggccatataa
     9901 tagtcaagga tatcataaag aataggtcaa gaataaaaaa tctcctaatt ggcaaaataa
     9961 taaaccaaga aggagatttt ttgattgaat atagctataa attagaatgt aattcttccc
    10021 attgttgata cagggattct aatttctctt ccagactaac tttggcatca taagcttctt
    10081 tggatttttc cggacttgta taaacttcct caagacataa taattcattg atttcctcaa
    10141 tttgacgttc tatatcatga atttggtttt caatatgctc tatttgggct tgcagttttc
    10201 tttgctgggc ttgttcttct ttctggcgga gccattcatc ttttgaggat tttgtttttt
    10261 cttccttttc catggaggct tttacatcat cttttatttg agctcttttt tccagataat
    10321 aatcataatt acccaagtat tcagtaattc cataaggaga gagttctaaa attttagaag
    10381 cggttttgtt gataaaatat cggtcatggg aaatatacaa tatggttcct gtatagtggt
    10441 ttatagcatc ttctaatatt tcttttgaaa tcagatcaag atgattggta ggctcgtcta
    10501 aaagaagaaa atttccttct gaaagcataa ttttgcaaag agagacccgg cctctttcgc
    10561 caccacttaa gctggctatt ggcttaaaga catcatcacc tgtaaataaa aaggctgcta
    10621 aaatattccg tatttctcct aaagtcaatg aaggataagc atcggatatt tcatctataa
    10681 tcgttttgtt aagagaaaga ttttgctgtt cctgatcgta gtagcttatc tttacgtttg
    10741 tgcccaggcg gaaactgcct tcatcataat ttaattgatt aagtaaaatg cggaataaag
    10801 tggttttacc gataccatta ggcccaataa gagctacttt ttctcctttt tttaaatgaa
    10861 aggagatatt ttgaaataac tttttaccat taaaggattt tgaaagattt tctacatgaa
    10921 gaacatcatt tccactttca aaccttggct ttaaggaaaa tttcattgaa gccggcagag
    10981 cagccggtgc ctggattttt tccatttttt ccaaagcttt ttcgcggctg atggctcttt
    11041 ttagagattt ttccctgtta aatgacctga gcgtttgtat aatttcttct tgtcttttaa
    11101 tttctttttg ctgatttata tattgcttta attgtatttc acgattaatt tgtttttgtt
    11161 tagcatagta agagtagtta cctttatata aactagcctg gttattttca atttcaatga
    11221 cttttgtgac cagtttatct aaaaaatatc tgtcatgaga tataatcaga acagcatagg
    11281 gataattttt taaaaactcc tcgagccatt cgattgctga gatatcaagg tggttggtag
    11341 gttcatccaa aagcaaaaga gaaggggaag ataataataa tttggccagg gctactctag
    11401 ttttttgtcc cccagataat tggttaatgg gttgacagag ttgattatca ttaaaaccta
    11461 agcctttaag aaccccttta atatggctct tatatccata tccgtttaat tcttcaaatt
    11521 gatgctggag ggctgaatat ttgtccatta atttagtaag tttacttccg ttaagagtac
    11581 tcatttcctt ttcgagcgta tggagattgg cttctaattc cataacatct ttgaagactt
    11641 taagcatttc ctcataaatt gtatgttcag aatccatttc tgcattttga ggaagatagc
    11701 caattacagc ctcttttgct ttaaaaattt caccactgtc aggagcaatt tctccagcca
    11761 gaattttaaa taaggtagat tttcccgcac catttacacc tgtaacagca actttttctt
    11821 tttcctctat ttgaaaacta atatccttaa ggatttgatc tattccaaaa gacttactga
    11881 tatctttaca tgataaaatc ataactagca cctcaactgc ttattttata taatgatatc
    11941 aaaaaagaat atggaaggaa aggctgattg acagtttttt taaataaatg ttataattta
    12001 aacatagttt taggaaagga gagaaaaaac attgcccgct aacagaagaa tatcaatggc
    12061 ggtgataaga cgcttaccaa ggtattatcg ctacttagga gaacttcttg agaatggtat
    12121 taagcgtatt tcatctaagg aactcagtga gaggatggga gttactgcct cgcaaattcg
    12181 tcaagattta aacaattttg gtggctttgg tcagcaaggt tacggatata atgtagagta
    12241 tttatataaa gaaattgcta aaattttagg tctggacaat aaatataaag tcatcattgt
    12301 gggtgtggga aatttaggac aagccttagc gaattatgta aaattcgaag aaagaggttt
    12361 tgtcctttgt ggcttatttg atgtgaatcc cagattaatt ggaatgacaa taagaggaat
    12421 tgaagtaaaa gacatagatg aaatggaggc ttttgttaaa gagaacaaga ttgacattgc
    12481 ttatttgacc ctgcctaaat ctaaagtccg aaaggtagca gaagatttag ccagttgggg
    12541 tgtaaaaggt ttgtggaatt tttctccggt tgatttagat ctttcctcgg atgtcgtagt
    12601 agaagacgtt cacttatcag atagcttaat gacattgtct tatcggatta accgtcaaat
    12661 gcagaagaaa gaagctcagt caaaaaaatt acataagtga ttggagtcat aaaaaaagat
    12721 ggagtatgct aagtttttta tcagccctgt cataggtggt atcattggtt attttaccaa
    12781 ttggcttgct ataaaaatgc ttttcaggcc tcacaaagaa ataaaggttt ttaatttaaa
    12841 acttcctttt accccaggtc tgattcctaa agagaaagag agaattgcaa gaagtgttgg
    12901 tacgaccaca gcaaaatatc ttctttctga agaagtgatt gtcagggctt tatcgagtga
    12961 agaactttta gagagtattg ctcatgttgc agcggatact tatcatgata tttgttataa
    13021 aaacaattac tctctgaatg atttcttaac ttttatttta gaagatgata aggaaaaaaa
    13081 tattgaatta atcagtgata aactgacttc atgggtaata gattatctta gtcaggagga
    13141 aaacttagat aattttgcaa gttttattgc cagttttatt gaggataaat tggatgtctg
    13201 cataggagac ataaaacttg aagattatat aaaaatagat tttttaatag aggaatccgt
    13261 taaacatcag cttatccatc aaatacttca tttaaaaaat cagctatgta aggataattt
    13321 ttcatcatcc agaattattc ctgatatttt tatagaaatg gctatggatt ttgcaaagag
    13381 ccaattatcg gaatggatgc ctttagcttt aggaattttg gaacagcctt ctgtagaaaa
    13441 taaaattaag gaaattttgc aggaatttat aaggaacaat ttgggaaaac tgactttaat
    13501 ttttgttgat gtcgataagc tttataacaa aattaaaagc tacttaaaaa gctgtattga
    13561 agatgagacc aatcaaaaag caatatttga acaaatagac atgtttatta agcagctatt
    13621 ggaaaagcct gtttgtcaat ggattgaaaa aattgaattt ctaaaaaatg agaaagaatt
    13681 tgaaaaaact tacgataaga ttatttttta tttaagtcag ggagaaaaaa ctgagacctt
    13741 aaaagaaaag ttaaaggagt acatcaacaa tcaaaaagaa aaaaatattt taagtatagt
    13801 aaaatcagtt aaaccagata ttgaccaggc aattgagaat cggattaaat cttatttaaa
    13861 acatttaatt gccagggaag aatggaaaga gtttatcaga aatctgatta aaaaagaatc
    13921 tgacaggctt ttatctactc cagtgaatca atggtttgaa ggattggaaa gtgtggtaga
    13981 agataagatt aagcatttta ccctgaaagc atataaagca tttgtcctta aacattcttc
    14041 agatttcatt aagcaattag atatatccga tattgttgaa aatagaatta tagaatttga
    14101 tacagatgag atggaagaga ttattctttc tgtagtggac agagagttaa aagcaatcac
    14161 ttggttagga ggtcttttag gccttgtaat agggtttgtt cctcttttgt tgagctaaca
    14221 agttatttgt tagctttttt tatttctcac gttatccttt ggttaattat taaaatgatt
    14281 taatttgaat gtaataagaa ttcaataatt attatatata attacgagat aagaggagct
    14341 tttaaaaagg ggtgaagata tgaaaaagtt attttcagga gtgtttttct ttattttttt
    14401 ctttcttttg ttttccagta atggtctggc aaaggccaga gaagaatatg gcttacccct
    14461 ggcttcatat aaaggagaaa aaggcgtaac aatagttagt ttttcctata actgggcaag
    14521 tcaagaaaag ttaaaagaag tttacgatga gttacttaag aattttcatg gagaagaaat
    14581 aaaatatttg acatacatct atatttatcc ggattccccg gaaggcgttt tagcctgcta
    14641 ttatgaagat tatgatataa acagtgaagg ggaatatgta tataaacctg gtcgctatat
    14701 agaaattttt aacggggatc aatatacgga tgtttcacaa tttgcccggg tccttgctca
    14761 tgaatacggg catcatttta cattatacta tttaatgacc aaagaaaata agcattttaa
    14821 tcagtggaag gaaaccaatt atgcaaagat aaggggactg gaaaaatata aagaagttgg
    14881 ttacttttca gttcaggata accgctatag ttatcagtgg gatgttgctg agatagctgc
    14941 caatgattat gttcagcttt ttggttcacc taatgcaaaa aagtctacag attataagga
    15001 tgtacaggaa cgggtcgaac aaaacattaa agcaagttat tattcgacgg atagttttaa
    15061 tttgtccccg caggaaaatc tttctattcc cttagcagca gatgttccag gtctttatct
    15121 ttattggtta ggtttagcgg gttacacttc tatggaacca gatattcctg ttaaaccgca
    15181 aataagcata aaaaaacata aagaaattat gtcaggctat tttcaatatg aagttacatg
    15241 gaatccaatt tcaaaatatg acgagtatga atatacttta gtggcttatc cagctgcaga
    15301 ttatattatt tttccaagac ccatcaaaac ggtatcccct ggggaaagca cgaaggctta
    15361 ttttgggagt gctatgcatc aaaatgaaaa tggtacagtc aatcttattt tagatgacca
    15421 atatgaagga gaattttatt tcaggctttt tattaaagat aaaagaggat ttattttttc
    15481 aacagagccc gttaagtatg attttaacaa ggacaaagta aaatctattt atagaatgac
    15541 agatattcct ccaaatcact gggccatcca ttatattgaa acgattattg acaaaaaaat
    15601 agcagaaggc tatgaggatg gcactttcag gcctgaagga gaaattacga aggtagaatt
    15661 tatggcgatg cttatgcgcg ctattcatta tgaacggatg cctgaggaag acggtaggga
    15721 ttggtttgaa aaagaaggat atttagaagc tgccagacag cttaatttag tgaatatatc
    15781 tgattgcaga gccaattata atcaattaaa tgaccccata acaagagaag aaatggcatt
    15841 tatggcagga caagtattaa agtatttagg ttatcgttat gagaaaggtt atcaggcaga
    15901 atttaaggat attggtcagg taaagtataa agatgaaata tctttggcag tttattatta
    15961 tattataaat ggctatcccg atggcagctt taaacctgaa aggcatgtga caagagcaga
    16021 agcatcaaag gttatttata aacttttgga ttttgtggat taaatttaat attacctgta
    16081 tttttttata caggtttatc accaagaaaa ccggggaata ttttcctcgg ttttcttggc
    16141 attcaattta ttcaatgcta aaattagaat agaacatttt tttggagaag aagaatgatg
    16201 aatattagaa aagctcagtt aaaagatata gaccacttaa tatttttgtg gaatcagtta
    16261 atcaattatc acaaggataa aaaactttat tttcctttgt ccagggactg gaaggaagaa
    16321 aaaagaagag aattagaatg ggttataaaa ggtgctttaa gtgtaattta cataatagaa
    16381 gaagataaac cgatcggtta tatcagggga actattcata aacttccatt attatacgat
    16441 ataaaatatg aaggcagcat cgaagaaatc ttcatcttat ccccatacag aagaaagggg
    16501 tatgggaaga aattaacgga agccttaatc caggacttta aaaagaagga agtcgagtat
    16561 attaatatta acgttgatat agataatgaa attggacaag ttttttggga cgctttaggt
    16621 tttaagacgg tttctcttaa aaaaagcttt aaactttaag gaggggaaga tgctgttttg
    16681 gaaaaaagat attaaattgg atgacggtct tttaaaaaaa gttccgattt taatacttga
    16741 taagaactgg cataatattt ttccccaaga aaagaaaact aaaaaaatac tccttttaga
    16801 agaaacactg agcaatttac ttaaagaaca agggcagctt aacaatgatt ataaggacta
    16861 cataaaatta aaaaaacaat tgatgggcga tattttaaat cttaccgatg aagcttttac
    16921 caatgaaatt gagatggcaa aagaaaaaat gggcaagaac aaaaaatata tcttagaaat
    16981 caataataag ttaaaaaaga ttcaaaaaag attagaaaaa ttacctgatg aaattgagga
    17041 aacgaattac agacttttaa aggaaagtgt catcatttcg tataaagaga tgaaagtgca
    17101 tcagaaaaaa ttaaaagaat taacagaatg gattgaaaaa acgagagaaa tactcaaaaa
    17161 caaaattgaa gaaagaacca ttcatgagga gaaagcaagt gaaatctatt cttacttaca
    17221 tgatcttgtt ggcctggaac ttattgaata tttagataaa aaattatggc ggtgacggaa
    17281 tggtaatcgg aattggtaca gatatcatcg aaattaaacg gatagaagaa gctgtaaaaa
    17341 agaattcaaa ctttatgaaa aaatgtttca cagccagaga acaagaactc tttgaaagca
    17401 aaaagaataa tttacatacg attgccggca cttttgctgc aaaggaagca gtatctaaag
    17461 cacttggaac cggttttgcc aaatttgaat taaaagatat tgaaatcgtt cgggatgaga
    17521 agggtaagcc ctgtgtattg cttcataata aagctaaaga aattatggaa caactgatga
    17581 tctcagagat tttactgaca atttctcatt cgagagaata tgctattgcg tatgcccttg
    17641 ctcaaaattg acttagaata ctttgataat agaaagagaa atggagggaa actatgaaaa
    17701 tatgcagtgg agaagaaatg aggatgattg atgaagcgac gattaacaaa gttggcattc
    17761 ctggtattat attgatggaa aatgcagtta ttgctgttgt taatgagata ataaaggata
    17821 tcaggcctac agtaaatacc agagtaactg ttttctgcgg acaggggaat aatggaggag
    17881 acggcttcgg cattgcaaga catttaagtc atatggattt aaatgtgaca gttgttttta
    17941 ttggagatta taattgcctt agaagtgatg caaaaacaaa ttttgatatt cttcaaaatt
    18001 taaggatccc tataaaaacc attagtccgg cttctgtagt tgatgatgaa atagcatatt
    18061 taattcaaag aagtgatatt gttgttgatg caatttttgg tacgggtttg tctaaaaaga
    18121 tcgaaggact ttacaaggat ttaattgatt taattaattt ttatggaaaa tatattatat
    18181 ctgtagatat tccttcaggg attgattcca gaacaggggc ggttttaggg aacgctgtta
    18241 aagcagataa aacggttact ttagccttgc ctaaagctgg cttgtatctt tatcccggta
    18301 cagattatgt cggtaaattg gttatagctg atataggcat acccagacgg attattgaag
    18361 aagcggagct taagatgaat gttttaacgg atgaggaagt caaaagctta attcctatcc
    18421 gtttcacaag gagcaataaa ggcagttatg gaaaagtgca agttattgct ggttctaagg
    18481 gaatgacagg tgctgctgcc ctaacctgtt taagtgcgta taaggtgggg gcaggaattg
    18541 tcagcctggg agttcctgca agtatcaaca acatattgga agaaaaatta acagaagtga
    18601 ttactgtacc gcttccagac aatgaaggga aattgtgtaa agagagtttt tatgccatca
    18661 aagcgcatct tgaaaatgca agtatgattg ctattgggcc aggattgggg caaaatgcag
    18721 aaattattga attaatggag cttattattc aaaattcaaa ggtgcctatt gtgattgatg
    18781 ctgatggtat taatgcggtt gcaagaaata ttgaaatgct aaaaaatata aaagtaccta
    18841 tcgttctgac acctcaccct ggagaaatgg ccaggctgac tggtcagacc attgaagaaa
    18901 ttttagaaaa gcctgttgaa attgtaagag atttttgtca taaatggaat gtaattttgg
    18961 tattaaaaga tgcaaaaaca ataataggac atcctaatgg agaaatatat attaatacga
    19021 caggaaatcc aggtatgtcc gtagctggct cgggtgatgt attaactggt attatttcag
    19081 gcttgattgc tcaaaatatg gatccctata aagcagcggt attaggtgtt ttcttacatg
    19141 gtaaagcagg agatttggca gcgaaggaat taggatatca cggtatgctg gcaggagata
    19201 tatgccgtta tacgccagaa gcgataaaaa tgtattatga aaaccaggag gtaagaaatg
    19261 aaggctaaag atatcatgga aagaaacgta ttagccataa aaaaagatgt atttgtaaaa
    19321 gatattgcaa aaataatgtt tgaaaataaa atcagtgggc ttcctgttgt agatgatgaa
    19381 aataaagtta tcggcgtagt gactgaaaaa gatttgtttt taaaagaaaa agaatctact
    19441 tttccggcat atattgaatt tttaggtagt ctactatttt ctgaagaaat aaaaaagtac
    19501 gatgaggaat taaaaaaatt atctcagacc actgctgaag aaattatgac acgggaggtt
    19561 cataccatta ctgaggatgc gacgattgaa gaaatcgcca atatcatggt taataaaaat
    19621 gtaaaccgtg tacctgttgt agacaaagga ggaagaattg tagggattgt cagcagggct
    19681 gatatgttaa agacaattat ataaaaaact tggggggatt tggtgacagg agcaaagaag
    19741 ctattacttc tttttatact tgcaacagga tgttttctta catcctgttc ttttaaggaa
    19801 aagtctaagt caccaataga aaaaattcat gaaaaactta catccatgga aaattatggt
    19861 tgcgttgcag atgtacgctt tatatccaat aaaggagaaa atacatataa gacaaaacag
    19921 cattataaaa tgacgggaga atacaggtta gaaatccttg cgcctgaaaa tttgcaaggc
    19981 ttagtaacgg tttatgatgg gcaaaagtta atacaatata atccccggat tcttggagaa
    20041 gttattactg aacagccaga gtctgagagt gctaatgaaa tttttttggg agccttttta
    20101 aagaattatc ttcaatcaga aggcgtagcc ttagaagttt ttaatcataa tagtgaaaat
    20161 tatacggttt tggaagcggt tattcctgat ggcgggaaat acttggcgac agaaaaatta
    20221 tggatttcaa ataaaagtct taatccagtc aaattagtaa tatatgataa agaaggaaaa
    20281 gaaagaatta ttgtcgaata cttagaattt aagtataatg ttaatttaga tgaaagtgtt
    20341 tttcgaatta acaaatccaa tagtgtgtaa ttttcagggg gaatataggg ggaacaccaa
    20401 tgacgagtga acattatcgc gtaattgcag aaatcaattt agatgcaatt tcaaacaata
    20461 tcaaagagat tcagaaaaat atctcaaaag atacagaaat aatggcagtt gtaaaagcag
    20521 atgcttatgg tcatggcgct atagaggtat ccagaatact aattgaaaac ggcgtgcaaa
    20581 gacttgcagt tgccatatta gatgaaggta aacagcttag ggaagaaggt tttaacgttc
    20641 ctattcttat tttgggttat acgcctgcag aacaggccgg tgagttggtt tcatatgatt
    20701 tgatacagac agttttcagc tttgaaatgg caaaggctat ttctgattca gccgtaaaac
    20761 aaaacaagaa agccaaaatt catttaaaaa ttgataccgg catgagcagg attggtttta
    20821 aacctgataa agaatctatt aaaaccatta aggaaataaa ccaattgcct aatatcaata
    20881 tagaaggaat ttttacgcat ttttcttctg cagatgaggc tgatacatct tttacccagc
    20941 agcaatttag tatttttaat gcttttatta aagcgcttga agaagaaggc ttgaaaattc
    21001 ctgttaaaca ttgctctaac agtgctggtt ttatgcttga tgaaaaaatg catatgaatt
    21061 taatccgtcc ggggattatt atatatggtt tatatccttc aaatgaggtg aataaggaaa
    21121 aaattcattt aaaaccagcg atgtctttaa agtctcaagt agtttttgta aaagaagtgg
    21181 aagaaaacac acccgttagt tatggtcgca aatttattac ttcaaagaaa acaaaagtgg
    21241 cgaccattcc tgtaggctac gcagatgggt attccagaag attgtcttct tcgggcagag
    21301 ttttaattaa cggtgagtat gcgccaatta ttggtaatat ttgtatggat cagtttatgg
    21361 tggacgtcag tcatatctct gatgtagaag tcggaacgga agttgtttta ataggacagc
    21421 agggacaaaa ttcaatttct gcagaagaaa tagcagatat tattggaacc attaattatg
    21481 aagtagtttg tatggttggt aaaagaatac ccagacttta tatagaaaat aaagaagtta
    21541 ttaagaaaat tatgtatgtt aatgaatcgg aaaaaataga tgaaaaaatt aattgatatt
    21601 cataaaagcg ttgtataaaa attgtttcat tggcaaaaat acattatgaa aatacttgta
    21661 tttaattaaa ggattttata tctttgattt ttaatcaagt attttccatt gagtctatag
    21721 actcatatga ataggagtga tttgcagtgt ccaaaaagaa taaaatcttt aagattaaga
    21781 agacctctga cgaagaacaa gatattatga ttaagggtta tcaggaaatg gctgaaatta
    21841 atttggattt atcaaaatta tgttttgaag cagaaagaga ggttgaaaag ataattgagc
    21901 aagtagtgga gtgtgagaga tgtggtggtt aaaagaggcg atgttttcta tgcagattta
    21961 agtcctgtgg ttggttctga acaaggaggc gtacgacctg tacttattgt acaaaatgat
    22021 attggtaaca aatacagccc tacagtaata tgcgctgcaa taacttctca aattaataaa
    22081 gcaaaattgc caactcatat tgaaattgat gcaaaatcct atgcattagt aaaagattct
    22141 gtagttttac ttgagcaaat aaggacaatt gataaaagaa gacttaaaga aaagatttgt
    22201 catttggaca aagaacttat gaaaaaagtt gataaaagtc taatgataag ctttggttta
    22261 tagtacataa caaatacccc cttttaaggg ggttatttgt ttgacaggtt ataggaagag
    22321 gtataaaata atagaagatg taaaaaaatc atggagataa atgaaaataa tgaaaaaaat
    22381 atatattatt gaatacctga ttttagataa aaaatcaatc aattttaatt aagaaataaa
    22441 ggagatatta aattgattat agatatacat acgcattgtt ttagtgatga cattgccaga
    22501 aaggctgtac cgctattagc ccaaaaggcc aacgttcttc cctatacaga tggaaccata
    22561 gcggatttaa agagatcgat gaaggaaaat ggcgttgatg tcagtgtgat tcaacccatt
    22621 gcaacaaaag caaaacaaac ccctgttatt aattcatgga taatgactat agaagattca
    22681 gagattatac catttggaac c
//
LOCUS       DAOSUD010000033        22241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1518_length_22241_cov_23.598395, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000033 DAOSUD010000000
VERSION     DAOSUD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 22241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22241
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_22241_cov_23.598395"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1519)
                     /locus_tag="PLG49_05790"
     CDS             complement(176..1519)
                     /locus_tag="PLG49_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ16869.1"
                     /translation="MSKMFKAKKLLAVLLSVSIFGSVLAGCGASKEENKSSGDTQTPG
                     KVTEQPKPQGKTKVTFWSPFSGGDGDIMKELVAEFNAQSETTEVEFLIFKSEEYYTKL
                     LAAMTTNSGPTVAINHITRIKEYVEDGLLLPLDSYAAEAGLNWGDFTGRLQEAAQVDG
                     SYYAVPLDTHLLLMHFNKDLLEEIGMLEADGTVKIERGEEGFFKFFEEVKKRAPEGVM
                     PISGTSQYGLPMYLWYTLLTQYGGDILDETGTKATLDTPENKKALSIMMRMVENDIWP
                     KNQKNGAEIFTAQKAVATINGVWALPIFESAEGLNFISIPFPQFTDTDSVYADSHSLV
                     FPKNDNISDEERKAAMEFAVWLTNNSAKWGKAGHIPSKPSVAQSDEYQSLLYRKDYAP
                     SAEYAKFYPQSTSIAGFTELIQRELAAMIAGEQDVDKTASNLQTEFQKLLDAKNK"
     gene            1862..3676
                     /locus_tag="PLG49_05795"
     CDS             1862..3676
                     /locus_tag="PLG49_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ16870.1"
                     /translation="MNTHCVSHPRPDFLRQNWMILNGNWSFDFDDHDEGEVKEWFNLP
                     YLKQKIEVPFVYQSEASGIEDKNFHPILWYQRTFKIPEDFKKSNIILHFGAVDYLAKV
                     WLNGTFIGEHEGGYTPFHFDITPHVKRNEENNLVVKVIDHKDPCQPRGKQYWEEYNEM
                     CWYQPSSGIWQSVWLENTGSTRMDYVKITPDLDNRLAIFEIFLEGEILENYEIKIALS
                     HKNSLPPRKKYFYHDFVEKSTNEKDTPFQIVSYTLNSNPARIAISFKEKEYIKDIHYW
                     SPENPHLIEAEISLYANDQLSDKVTTYFGMRKIELKGDEIYLNNQPYYQKLILDQGYW
                     PDTHLTPPSDEAIKADIEYAKMMGFNGARKHQKIEDPRYYYWADILGFLVWGELPSAY
                     DYNDKSIKNLSRDMHEFIRRDYNHPSIITWVPLNESWGVRNIYYDKSQQAFAASLYYQ
                     IKALDQTRLISNNDGWEIVNQTDIYGLHDYTAYSENLSENYESKEKLMTHHSQYRPSL
                     ALGHKYQNKPLLITEYGGIAFQDEADQSWGYFGKVKSKEEFLERFRNITLGFRKIPYI
                     RGYCYTQLTDVYQEKNGLLTMDRKFKIEPELIKKIIEK"
     gene            3712..4659
                     /locus_tag="PLG49_05800"
     CDS             3712..4659
                     /locus_tag="PLG49_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOQ16871.1"
                     /translation="MPELITLGETMASFSPQTSGPLRYISDYKIHIAGAESNVAIGIT
                     KLGHSAGFISALGNDEFGHYILNFIRGEGVLCDKVKFDSEHPTGIMFKETSSSETRVF
                     YYRENSAASFLSPQDIDEVYIARAKILHLTGITPILSSSCKEALYTAMAYAKKHKLLI
                     SFDPNIRKKLWKNKDYRPLIKDILFQSNIILLGLDEAETLFETNKEDEILDILLSKDN
                     IQYVAIKNGAKGAWVATKNKTGFIPPYPCSPVDPIGAGDAFNAAFLSGILEEKDILLC
                     GQMAAIAGALATETHGDVEGLPTKEKLEALLNHKKEIDR"
     gene            4681..5298
                     /locus_tag="PLG49_05805"
     CDS             4681..5298
                     /locus_tag="PLG49_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOQ16872.1"
                     /translation="MKDLFQKHKICAILRNIPLEKTIDYVTAAFNGGIRLFEVAMNSP
                     HAEKQIQILTDKFGDRALIGAGTVINKELCLRAKNAGAQFFLTPSVVIETLEFCAANQ
                     IKLLPGVLTPTDVSVCLNYGYKTFKLFPACDMPQNYIKSLLGPFDNTNYVAVGGVKLE
                     NASDYLKQGFIGVGIGGSLIPKEFIEKEEWEKASQLIRENIQKLS"
     gene            5314..6462
                     /gene="dgoD"
                     /locus_tag="PLG49_05810"
     CDS             5314..6462
                     /gene="dgoD"
                     /locus_tag="PLG49_05810"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HOQ16873.1"
                     /translation="MKITNINSYLVRPRWHFIEIETDEGISGWGEPIIEGKAYTVKAC
                     IEEMKSYLIGQDPMKIEDIWNSLYRAAFYRGGPILMSAIAGIDQALWDIKGKYYNAPV
                     YELMGGPCRDKMRVYSWIGGDRPSDVAKAAKEKQEQGFTAIKMNATEELQYIDTYDKI
                     DAVLERVASIREATGKYFGIAIDFHGRVHKPMAKILAKKLEEFDPMFIEEPVLSENME
                     AFSEVAKASNIPIATGERLFSRWDFKRLFQLGCVDIIQPDLSHAGGITEVKKIASMAE
                     AYDIALAPHCPLGPIALTSCLQVDATCHNAVIQEQSIGIHYNKDRSVLDYVLNPEDFV
                     FKEGYIDLPKKAGLGVIMNKELIIEENQNPHSWKNPIWRHQDGSIAEW"
     gene            6478..7599
                     /locus_tag="PLG49_05815"
     CDS             6478..7599
                     /locus_tag="PLG49_05815"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOQ16874.1"
                     /translation="MKVVVAIDSFKGSLSSIEAGNAIKEGILRAKENAQVVVKPLADG
                     GEGTTDAFIEGLGCEKIYVDVTGPSGQKVTAYYGYQKDSNTAIMEMASAAGITLLEKK
                     DPMHATTYGVGEMIKDAIQKGCRHFIIGIGGSATNDGGIGMLQALGFEFFDEKQNPVG
                     QGGQALGKIKSISDLNKLKELDECHFKIACDVTNPLYGPSGATYIYGPQKGVTDDIKD
                     LLDEGMKNYAQRTKEFNGTDYANTPGAGAAGGLGFAFLSYLKGELESGIELILKAIKL
                     EEEIKDADYVITGEGRLDHQTAMGKAPVGVAKLAKKYGAKVIALAGSISPGAKKCNEE
                     GIDAYFCILNSVVTLEEAMNKETATENMINTTEQIFRLL"
     gene            7862..8035
                     /locus_tag="PLG49_05820"
     CDS             7862..8035
                     /locus_tag="PLG49_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16875.1"
                     /translation="MSDSRSYEEIVEDAKDKQINAIKELSKDHSPKLKEKIIDSIKDR
                     AEMIHYIEENLMK"
     gene            complement(8091..8834)
                     /locus_tag="PLG49_05825"
     CDS             complement(8091..8834)
                     /locus_tag="PLG49_05825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ16876.1"
                     /translation="MVDVKGKWALITGASRGIGYQIAIFMAKQGCNLILHSRNLQNTK
                     KVEEEVKALGVEAYSVQAELSEIEEVYVMLNEIDKKGMPVDIVFNNAAVQVAYRADFW
                     KTPLEDFDLSFKINLIPAVVICYHFIPKMIERGFGRVINVTSGIKNEPEQAGYSASKA
                     ALDKFTIDLGSKLEGTDVMINLTDPGWCRTDLGGPKAPNPVESVIPGIVVAAFLDDKK
                     SGRLFAAQDFKGMSLEEAVKKAQTIDSNY"
     gene            complement(8970..>9971)
                     /locus_tag="PLG49_05830"
     CDS             complement(8970..>9971)
                     /locus_tag="PLG49_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ16877.1"
                     /translation="NGSFGFNKIIGITENIRPEQINHFRDNSSYLELLYTAEWTGLIE
                     SSYNQAKVNIIQTEKNYPVFIHTPVLKGSFLTGRSDEDYEIVLEEEVAYQLFKSTDVI
                     GLTVNYLGQNFKIVGITENRPPFLNHLSHRENLGNVYIGTKEVQNGSRKKTESIQIAF
                     KKENEELALEEFASMTPDQLGMQYMIKAYEIKKQIEIKNWIFLLLFSIMAFYYLALAI
                     IKFVKKIMDRIKERYQEAYLGELIYKSYKEILLTFMALAVYLLAIFGLLKIFPYTYEL
                     LTTNVIKIWGQEFKEALFNVGAGSGVLSLFSISILILDIFYIFLFCLWFNAVKKFRD"
     assembly_gap    9972..10071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10227..11525)
                     /locus_tag="PLG49_05835"
     CDS             complement(10227..11525)
                     /locus_tag="PLG49_05835"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOQ16878.1"
                     /translation="MEELNPVAMLKKKILSKIILVFLSVMLLLTFFSKTIINLSLPQV
                     KSEKISGGNLLFSIRAEGQVLPDEIREVYSKKNLRIKEILVEEGDQVEAGDTIILLEG
                     SEDSSAMEKEIQLKKLQLQLEEIKNQPALVDLSIYEKSLEEAKRNLDREKENYEKIKT
                     LYESGAESAKSLQEAEYRLEDSRQNYELSLSQLEQKKLEKDAEEKERILEIKKLEYDM
                     ELIRSELEKDTDTGGRITAPCSGIIQAINCRKGALADAYNSLCSIINTTEKSKFVAEV
                     NIDEVKYLRIGEQVDLKFSSFGNKVIKGQIEKILGPDEKNPSKRKVVISYEEEKVLPW
                     EWGTFYYSSQSPHYKMIIPKKAVGEEMGRNFVYVLKSKETSLGEIFYAEKKYIEIEQE
                     DFTNCAVSSGLDGSEEIIVYSSKPLFDGIRVRKMYEGEEE"
     gene            complement(11557..12414)
                     /locus_tag="PLG49_05840"
     CDS             complement(11557..12414)
                     /locus_tag="PLG49_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ16879.1"
                     /translation="MVRSRFSAKLIKTVFLAVVSLFFLYPILYIFGASFMMPQEVTER
                     IIAVKTGKSSQYISFDFIPDIFSIRQYKRILLNQATYLSMFWNSLKIVLPLVIGQVAV
                     GSMAAYVFAKLKFPHREKIFFLYIITMLMPFQVTLVPNYLVMDYLGLLDNPLSLILPG
                     IFSAFGVFLLRQFMTYIPDSCIDAARIDGAGLVRIFFYIVLPMVKHGIAALVILTFIE
                     YWNMIEQPLLFLSDVQKQPLSTYLPVIGENDLEIAFAASFIYMLPVLLLFLYLEEDLV
                     RGIQYSDIQ"
     gene            complement(12428..12796)
                     /locus_tag="PLG49_05845"
     CDS             complement(12428..12796)
                     /locus_tag="PLG49_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ16880.1"
                     /translation="MVLFLGGLNNIPKEYYEQAALEGAGKCWIFKNITLVYLTPVFFF
                     VLIMSLLSSFKVFREVYLIAGVYPHESIYLLQHYMNNVFFTLDYQKLTSAAYLTAIFI
                     YGLVYIILKLDKKFSQKIGI"
     gene            complement(12815..13315)
                     /locus_tag="PLG49_05850"
     CDS             complement(12815..13315)
                     /locus_tag="PLG49_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ16881.1"
                     /translation="MQKFKNCLKNKQENFIVTFIFLAPSLLGFSVFYIIPFIVAGVYS
                     FFDSSFGASFVALRNYMDLINNPVFRLAVKNTLVFTVISVPLSVFISLGLALILNGDV
                     FFKSFFKTTFILPLAIPTASVALLWNMAFQNRGMVNGLLSRTALPTVNWFNSSWTIIV
                     IVLMYI"
     gene            complement(13544..15856)
                     /locus_tag="PLG49_05855"
     CDS             complement(13544..15856)
                     /locus_tag="PLG49_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ16882.1"
                     /translation="MKKILAFIIIFIFLINICGCSSNKGNETADNENHNMIVEPQENI
                     EITVEDEDITNLIHFDFAVQNIKMASDGRYILYNMDNKSLVVMSKDNQKLVEIPPDEN
                     FPDTVKNTSYYLFGMVFTLDKNDYLYVLWQKEVEEGSQPKVYQKICKYDLAGSKLEEI
                     YLGSHLIEENDYLHQIDFELDGDGNYYLLYLGKKVVAYTPEGKKFLEFNLNNDIYFLE
                     TDEENNLWLAGDNGSIKKVDPKKNEVIFEIQAGELGGCKYIEKDKSLYVFDDRGINQF
                     SSDGREAGYILRMSDLKNVDHSMGNMLAVDMNDNKDIIAILLDYKTDTTKLVKLNRKL
                     EEIISSDNNKVTLTITIPSYNAYLETAARQYEKENPNVKIQLNQYDAEINFPKVPEDE
                     ALKKAYDEYEKKLDDYVTQISAEIMAGKGSDILFLDYLPYYEYIDKNLLLDLTERINN
                     DSDFNINELNQNIIRNIQVNNKLYFIPMSYNIDTYIVNKDWLDSGGIKVKNGICQYED
                     FEAAVKNLKATNQPVFAGYFINNEYFMKSLIFMNNNQLIDYSKKKAYFNTDKVIALLN
                     LGKEMIDEAMISDPAEHLLTGAADANLAYEQIYDYRYSTTEMVRSVGKEFEIFRVSDD
                     DRDNGYTFTINSSLGISSQSKNQEEAWKFLKYLLSEELQSQEKLIVLPVNEKARQNKM
                     ERERKLLEKDNDPENGFLKEVYGIELGNIDSYYKKINEVAGNLGLYHYRDFNIEQIMD
                     EEIRKFLDNQQSAKETAEAIQRKVEIYLNE"
     gene            complement(15971..17371)
                     /locus_tag="PLG49_05860"
     CDS             complement(15971..17371)
                     /locus_tag="PLG49_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ16883.1"
                     /translation="MKRLSFRFTMVFGIMVFISCVIVDSLLIFTYYKKELKQLEDNIQ
                     KNGNIIASFRAYYGDSLELMDLVNRYEENMSGRILILNDDGEVIIDSQDILVGELISN
                     KEIRKTQETKTMASGYYYSNGKKMLMMTIPVFQPVGTYYRELNYRDGMVLISVYVQDV
                     IENVWDYGKKVLEFSLIIWIAIIILSCWAGYYVEKPVKKLIDASKRILEGDLKVQVNI
                     DRKDEIGILAKTFNQMCRQLDFIESNRRTFISNVSHELKTPLASIQALIESLDENDSI
                     ETYQEYLADISKEVNRLSKLVQSLLTASRLEEIKLDKEYIPIKDFVNEIIKQISALSR
                     DKKIEWYNECDENIEILADRNGLKEVMINLMDNSIRYGKENGFIKIYTQKEDSYLILG
                     VADNGIGIPEESQEHIFDNFYTVNRSRARKQGGSGVGLYIVKKIIDLHRWEIKIESDI
                     DQGTDIQIFLKDFRVV"
     gene            complement(17374..18063)
                     /locus_tag="PLG49_05865"
     CDS             complement(17374..18063)
                     /locus_tag="PLG49_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ16884.1"
                     /translation="MNILIVDDEQALVKGLKKSLTKEGYNVFTAYDGKEALELINNEK
                     IDCILLDLMLPEIDGITLCKKIRQTMDVPIIMLTAKDDYIDKIVGLEMGADDYVTKPF
                     HTKELIARIKAILRRVNSMSVVKEEEDVIEVGAVKVCASSRTAYLNGKEIPLTAKEFD
                     ILYLLMKERGKVFSREMLYELVWHEVSFDTRTVDVHIRKIRQKIEDDPANPYYLKTKW
                     GVGYYFRKDVI"
     gene            complement(18267..19151)
                     /locus_tag="PLG49_05870"
     CDS             complement(18267..19151)
                     /locus_tag="PLG49_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16885.1"
                     /translation="MDFIEAKNILSAYSTNNPWFGVNYNMNIYKGCCHGCIYCDSRSD
                     CYGVENFDKVRAKKNALEILAKELKSKRKTGVVGTGAMSDPYNPFEKKYALTRGALEL
                     IHRYGFGAAIATKSDLVRRDIDLLKSIQEHSPVLIKITITTCDDDLCRIIEPNVALAS
                     KRFECIKELSDKGIFTGILMMPVLPFIEDNEENIKGIIQFAHKSGAKFIYPAFGVTLR
                     QNQREWYYNRLDEYFPGFKEKYIQQYGNAYQCPSPKAKKLWYLLKGECERLGMLYKMK
                     DIIFAYKQWYQYSQLSLF"
     gene            complement(19153..19911)
                     /locus_tag="PLG49_05875"
     CDS             complement(19153..19911)
                     /locus_tag="PLG49_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HOQ16886.1"
                     /translation="MNIIRVNMDNLEQEHICCAISNTKGDPAVAAKKAWLKDRFAEGL
                     VFKKLDVRGKVFIEYIPAENAWCPIDADGYMFINCLWVSGQYKGQGYSNLLLKECIED
                     AKAMKRVGLVILSSDKKRPYLSDPKYLKYKGFIVSDKANPYFELYYLPFDENIKEPKF
                     KDYARAGKIHKKGFVLYYSNQCPHAEKYAIIIEKIARERGLDFTLIRYESKEEAQNAP
                     SPFTTYSLFYDGEFVTNEILSDKKFIKFLEEKGI"
     gene            complement(20012..20914)
                     /locus_tag="PLG49_05880"
     CDS             complement(20012..20914)
                     /locus_tag="PLG49_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOQ16887.1"
                     /translation="MQINRLFEMVYILLRKKTVTAKELAEHFEVSTRTIYRDLDTLSS
                     AGIPIYTSKGKGGGISLIENYVLKQSLFSEEEQKELLMSLQGLNAINTPNMESILNKL
                     SAIFNQESIHWIDVDFSGWGNSKRDKEKFKQIKEGIFNKKLMSFDYFSSSGEKTIRLV
                     EPLKLFFKGQSWYLYAFCRSRNDFRFFKLTRMKNLNLLEEQFERKIPAETGQVLKMDD
                     DKAVKLVLKIDAKMAYRVYDEFDEELIKKQNDGTFIISTVFPEGDWLYSYILSYGSYA
                     EVLEPERIRLIIMKNFEDGLKKYL"
     gene            complement(21061..21714)
                     /locus_tag="PLG49_05885"
     CDS             complement(21061..21714)
                     /locus_tag="PLG49_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOQ16888.1"
                     /translation="MFKFKDIIVRKHFSLGEEIAHSISHGAGLLLGIAALVLLVIKGA
                     LRGNPLYIISMLIYSVSLIFLYTNSMFYHAFPEGKAKNIFERLDHSSIYILIAGTYTP
                     FYFFAIGGIIGIVICCIQWLLAILGVIFKVLWIEKYTKIHVLIFLIMGWMIIFFAATI
                     YKTVSLGGFVLLLSGGLCYSIGVLFFVFDWFKYHHFVWHLFVLAGSILLFFTIYFFI"
     gene            complement(21870..>22241)
                     /locus_tag="PLG49_05890"
     CDS             complement(21870..>22241)
                     /locus_tag="PLG49_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16889.1"
                     /translation="FKILFKGLFFVIRKIGRKEESISHKKLHRIYIIMTIENAIVAFT
                     FYYLILAPVTWTRLSVMIQCMVLAIAVVISLINTVIMIKQWDKKNLTKPSKRRYIATV
                     VESCYISLFIIYWQLFNFWSC"
BASE COUNT         7232 a         4262 c         3372 g         7275 t
ORIGIN      
        1 aaatcccaaa taaggaatca gaaaaagcca agccgcaata ttttctttta ttatattttt
       61 aagcctctca ctgtttgtgg ctcctttcat atttaacctc ctgactttat actgcttatt
      121 aatgaaggtg ccttaacaag tggcaccttc attaacttaa tattattatt tatttttact
      181 tgttcttagc gtctagtaat ttttggaatt cagtttgaag attacttgct gttttatcta
      241 catcttgttc accagcaatc atcgcagcaa gctctctttg aattaattct gtgaaacctg
      301 caatacttgt tgactgtggg tagaatttag catattctgc tgatggtgca taatcttttc
      361 tgtaaagaag actttgatat tcatcagatt gtgctacaga tggcttactt ggaatatgtc
      421 cggctttacc ccattttgca gagttgtttg taagccatac tgcaaattcc attgctgctt
      481 ttctttcttc atcactgata ttatcatttt tagggaatac taaagagtgt gaatctgcat
      541 atacagaatc agtatctgta aattgtggga aaggaattga aataaagttc agaccttctg
      601 cagattcaaa aataggtaat gcccatacac cattaatggt tgctactgct ttttgagctg
      661 taaagatttc tgcaccattc ttttggttct ttggccaaat atcattttca accattctca
      721 tcataatact taaagccttt ttgttttccg gagtatctaa ggtagccttt gttcctgttt
      781 catctaaaat gtccccaccg tattgtgtta acagggtgta ccataaatac attggtaaac
      841 catattgaga agtaccggaa ataggcatta caccttcagg tgctcttttc ttaacttctt
      901 caaagaattt aaagaagcct tcttctcctc tttcaatttt tacagttcca tctgcttcta
      961 acatgccaat ttcttctaat aagtctttat taaaatgcat taataataag tgagtatcca
     1021 atggcactgc ataataagaa ccatctacct gagctgcttc ctgaagtctt ccagtaaaat
     1081 caccccaatt aagacctgct tctgctgcat agctgtctaa tgggagcaat aatccatctt
     1141 ctacatattc tttaattctt gtgatatgat taattgctac agttggtcct gaattagttg
     1201 tcatcgctgc taataatttt gtgtagtatt cttctgattt aaaaattaaa aattctactt
     1261 cagtggtttc actttgagca ttaaattctg ctactaattc tttcataatg tctccgtcac
     1321 cgccactaaa tggagaccag aaagttactt ttgttttacc ctggggtttg ggttgctcag
     1381 ttacttttcc aggtgtctga gtatcgccag agctcttgtt ttcttcttta gaagctccac
     1441 aacctgcaag tactgaacca aaaatactta cagacaatag tactgctaat aactttttag
     1501 ccttaaacat tttgctcatt tttttcctcc attcttcttg ttaatttctt tgccttaacc
     1561 ccctgacaca acaacaactt gaaagatttt agtgtggagt tgttagttgt attacaggta
     1621 gtcgtatcct tagtataaca aaatatttgt aaatgtcaat ggttttttcg acattttttt
     1681 atgttttttt gtataatatt acaaagcatt aggtttaata agctaaaatc ctttgactta
     1741 tttaatatta ttcttctttt ttacacagaa tatacatgaa tattcatttg acacattata
     1801 aaaactatgt tactgatagt aatatattat ttaattttta aacttcagga ggtgcagcaa
     1861 catgaatacg cattgcgttt cccatccaag accagacttt ttacgacaaa attggatgat
     1921 tctaaatggc aattggtcct ttgactttga tgaccatgat gaaggtgaag ttaaagaatg
     1981 gtttaatctg ccttatttaa agcaaaaaat agaagttcct tttgtatatc aaagcgaggc
     2041 cagtggtatt gaagataaaa attttcatcc tatcctctgg tatcaaagaa cttttaaaat
     2101 cccggaagat ttcaaaaagt ctaatatcat cctgcacttt ggagctgtag attaccttgc
     2161 caaagtctgg cttaacggga cttttattgg tgaacacgaa ggcggttata ccccttttca
     2221 ttttgatatt actccacatg taaaaagaaa tgaagaaaat aacctggtgg taaaagttat
     2281 agatcataaa gatccctgtc agccaagagg aaaacaatat tgggaagaat ataatgaaat
     2341 gtgctggtat cagccttctt caggtatctg gcaatccgtc tggctggaaa acacaggctc
     2401 tacccgaatg gattatgtaa aaattacacc tgatttagat aataggcttg ctatattcga
     2461 aatttttttg gaaggcgaaa tcctggaaaa ttacgaaatt aaaattgcac tttctcataa
     2521 aaacagtctt cctccaagaa aaaaatactt ttaccatgac tttgtcgaaa aatcaacaaa
     2581 tgaaaaagat actccttttc aaattgtatc ttatacttta aacagcaatc ctgccagaat
     2641 tgctatttca ttcaaggaaa aagaatatat caaagacatc cattattgga gtcctgaaaa
     2701 cccacattta atcgaagcag agatttctct ttatgccaat gaccaattat cagacaaggt
     2761 aacgacctac tttggcatga gaaaaatcga gctaaaaggg gatgaaattt atttaaataa
     2821 tcagccctat tatcaaaaac tcattcttga tcagggttat tggccggata ctcatctgac
     2881 ccctccttca gacgaggcaa ttaaagctga cattgaatat gccaaaatga tggggtttaa
     2941 tggggcacga aaacatcaaa aaatagaaga tcccagatac tattactggg cagatatctt
     3001 aggcttttta gtttggggag agttgccctc tgcttatgat tacaatgata aatccataaa
     3061 aaatctctcc cgggatatgc atgaatttat ccgccgggat tataaccatc cctctattat
     3121 cacctgggtt cccctgaatg aatcatgggg tgtaagaaac atctattatg ataaaagcca
     3181 gcaagctttt gctgccagtt tgtattatca aattaaggct ttagaccaga cccgcttaat
     3241 cagtaataac gacggctggg aaatcgttaa tcaaacagat atttacggct tacatgatta
     3301 tacggcatat agcgaaaact taagcgaaaa ctacgagtcc aaagaaaaac ttatgacgca
     3361 ccattcccaa tacagacctt ctttggcgtt aggacacaaa tatcaaaata aacctcttct
     3421 tatcactgaa tacggtggaa ttgcttttca ggatgaagcc gatcaaagtt ggggatactt
     3481 tggaaaagta aaatccaaag aagagttcct cgaaagattt cgtaacatta ccttaggctt
     3541 tcgtaaaatt ccatatatta gaggttattg ttacacacag ttgactgacg tctaccagga
     3601 aaagaatggt cttcttacaa tggacaggaa gtttaaaatt gaaccagaat taattaaaaa
     3661 aatcattgaa aaataatgaa caaactttta ccagaaagaa gggaaatatc catgccggaa
     3721 ttgattactc tgggagaaac tatggcgtcc ttttctccac aaacttccgg acccctccgc
     3781 tacatctctg actataaaat tcatattgcc ggtgccgaaa gcaatgtggc cataggaatt
     3841 accaaattag gacacagtgc tggttttatt agtgctttag gcaatgacga atttggccac
     3901 tatattttaa attttatacg gggcgaagga gttttatgcg ataaagtaaa atttgattcg
     3961 gagcatccga ctggcattat gtttaaagaa accagttctt cagaaacaag agtattttat
     4021 tatagggaaa attcggcagc cagttttctc tctccccagg atattgatga agtatatatt
     4081 gccagggcta aaatattaca tttaacaggt attactccca tactcagttc ttcctgcaaa
     4141 gaagctcttt atacagccat ggcttatgca aagaaacaca aactccttat cagctttgat
     4201 ccaaatatca gaaaaaagct gtggaaaaat aaggactaca gacctttaat taaggatatt
     4261 ttatttcaat ccaatatcat tcttttaggt ttagatgaag ccgaaacgct ttttgaaaca
     4321 aataaggaag atgaaatcct tgatattctt ttgtcaaaag acaatattca gtatgtagcc
     4381 attaaaaatg gggctaaagg tgcctgggtt gctacaaaaa acaaaacagg atttattcct
     4441 ccctaccctt gtagtcctgt tgacccgata ggggccggag atgcatttaa tgccgctttt
     4501 ctttcaggca ttttagaaga aaaagatatt cttttatgtg gacaaatggc agccattgcg
     4561 ggagcccttg ccactgaaac ccatggagat gtcgaaggac tcccaacaaa agaaaagtta
     4621 gaagccttat taaatcataa aaaagaaatc gaccgttaat attaaagaaa ggatgtccct
     4681 atgaaagatc tatttcaaaa gcataaaatt tgtgccatac tgagaaatat tcctttagaa
     4741 aaaactattg attacgtcac tgctgctttt aatggcggaa ttcgcctttt tgaagtggcc
     4801 atgaattctc ctcatgcaga aaaacaaatt caaatactta ccgataagtt tggagatcgg
     4861 gccttaatag gtgcgggaac tgtcatcaat aaagaactct gcctcagagc taaaaatgca
     4921 ggtgcccagt tcttcctgac accttcagtg gtaatagaaa ctttagaatt ttgtgctgcc
     4981 aatcaaataa aattgcttcc tggcgtatta acccctacgg atgtctctgt ttgtcttaat
     5041 tatggctata agacttttaa actattcccg gcttgtgata tgccccaaaa ttatataaaa
     5101 agtcttctgg gaccttttga taacacaaac tatgtagccg taggaggagt taaactagaa
     5161 aatgcttcgg attacctaaa acaaggtttt attggcgttg gtattggagg cagcttaatt
     5221 ccaaaagaat ttatcgaaaa agaagaatgg gaaaaagcct cccagttaat ccgggaaaac
     5281 attcaaaaac tttcataaga cggaggatgg gttatgaaaa taacgaatat taattcttat
     5341 ctggtcagac cccgttggca ttttatcgaa attgaaaccg atgaaggcat aagcggttgg
     5401 ggagaaccaa tcatcgaggg caaggcttat acggttaaag cctgtataga agaaatgaaa
     5461 agctatctga ttggtcagga tcccatgaaa attgaagata tttggaacag cttataccgg
     5521 gctgcttttt ataggggtgg ccctatcctg atgagtgcca tagcaggcat tgatcaggct
     5581 ttatgggata ttaaaggcaa atattataat gcccctgtct atgaattgat gggcggacct
     5641 tgcagggata aaatgagagt ttattcctgg attggtggag accggcccag tgatgtggca
     5701 aaagctgcaa aagaaaaaca agagcaaggc tttactgcta taaaaatgaa tgcaaccgaa
     5761 gaattacaat acattgatac ttatgataaa atagatgcag tactggaaag agtggcttct
     5821 attagagaag ctacaggtaa atattttggg atcgccattg attttcatgg aagagttcat
     5881 aaacccatgg caaaaatcct tgccaaaaag ctcgaagaat ttgatccaat gtttatagaa
     5941 gaacccgtac tttctgaaaa catggaagca ttttcagaag ttgcaaaagc cagcaatatt
     6001 cctattgcaa caggagaacg tttattttcc cgctgggatt ttaagcgtct ctttcaatta
     6061 ggctgtgtag acattatcca gccagattta tcccatgccg gaggcataac agaagtgaaa
     6121 aaaatcgcct ccatggctga agcctatgat attgctttag caccccactg tccattagga
     6181 cctattgcct taacgtcttg tcttcaagtg gatgccactt gccataatgc cgttatccag
     6241 gaacaaagta ttggcattca ctacaataaa gaccgcagcg tacttgatta tgttttaaac
     6301 ccggaagact ttgtttttaa agaagggtat attgatttac ctaaaaaagc aggtctgggc
     6361 gtcatcatga ataaagaatt aatcatagaa gaaaatcaaa atccccattc atggaaaaat
     6421 cccatatgga gacatcaaga tggctctatc gcagaatggt aataaaggag gaaaaatatg
     6481 aaagttgttg tagccattga ttcatttaaa ggaagtttat cttctattga agcaggaaat
     6541 gccatcaaag aaggcatctt gcgcgcaaaa gaaaatgcac aggttgttgt aaaaccctta
     6601 gctgacggtg gtgaaggcac aaccgatgct tttattgaag gcttaggttg cgaaaaaatc
     6661 tatgttgatg taacaggacc atcgggtcaa aaagtcactg cctattacgg ctatcaaaaa
     6721 gacagcaata ccgccatcat ggaaatggct tctgcggctg gcatcacttt acttgagaaa
     6781 aaagatccca tgcacgccac cacgtacggt gttggtgaaa tgattaagga cgccatccaa
     6841 aaaggctgcc gtcatttcat cattggcatt ggcggaagcg ctaccaatga cggaggaatt
     6901 ggtatgcttc aggctctggg ctttgaattt tttgatgaaa agcaaaatcc tgtaggtcaa
     6961 ggtggtcagg ctttgggcaa aataaaaagt atctccgatc taaacaaatt aaaagaactg
     7021 gatgaatgcc attttaaaat tgcctgtgac gtcaccaatc ccctttacgg tccttctggc
     7081 gctacataca tttatggtcc gcaaaaaggt gtaaccgacg atatcaaaga tttgctggac
     7141 gaaggtatga aaaattatgc acaaaggaca aaagaattta acggaacaga ctatgccaac
     7201 acgcctggtg ctggagctgc cggaggctta ggctttgctt tcttaagcta tttaaagggt
     7261 gaattggaat ctggaatcga acttatatta aaagctataa aattggaaga agaaataaaa
     7321 gatgccgatt acgtcatcac tggtgaaggt cgtctggacc atcagaccgc tatgggcaaa
     7381 gcacctgtag gcgttgcaaa actggctaaa aaatatggag caaaagttat tgccctggct
     7441 ggaagcatca gccctggcgc taaaaagtgc aatgaagaag gcattgatgc atacttctgt
     7501 atcttaaact ctgtagttac tttagaagaa gccatgaata aagaaacagc tacagaaaat
     7561 atgattaata caactgaaca aattttcagg ctactttagt aaattaacga ctatattatg
     7621 ataataactt aacaaatctt cgtttgataa aaatctgaca aactataaaa gccgcttcat
     7681 atatcgaaat atttgaagcg gcttttattg aatttactta aaaaatgcgt ccaccattgc
     7741 attctttttg cattgtaatt aaaaattctt attatttata aatttaatgc ctggcaatat
     7801 taatctctcg aatggaaata ataataactg taaacatttt tatctgaaag ggggcttttt
     7861 aatgagcgac tcccgttcat atgaagaaat tgtagaggat gcaaaagata aacaaatcaa
     7921 tgccattaaa gaattgtcca aagatcacag tcccaaatta aaagaaaaaa ttattgacag
     7981 tatcaaagac agggctgaaa tgattcatta tatcgaagaa aacttaatga aataatctgt
     8041 tagaaaaagg tatcactgtc tgccttgaac agactggtga taccttttta ttaataattg
     8101 ctgtcaatgg tctgagcttt tttcactgcc tcttctaaag acatcccttt aaaatcctgg
     8161 gctgcaaata atctgccaga ttttttatca tctaaaaaag ccgcaacaac aattcctgga
     8221 ataacactct caacaggatt tggtgcttta ggaccgccta aatcggttct gcaccagcca
     8281 ggatcggtta aattaatcat tacatctgtt ccttctaatt ttgagcccaa atcaatggta
     8341 aacttatcta atgctgcttt acttgcagaa tatcctgcct gttctggttc attttttata
     8401 ccactggtca cattaataac cctgccaaag cctctttcta tcatcttagg gataaagtga
     8461 tagcaaatca caactgcagg tattaaatta atcttaaagc ttaaatcaaa atcttcaagg
     8521 ggtgttttcc agaaatctgc cctataagcc acttgtactg ctgcattatt aaacacaata
     8581 tccacgggca ttcctttttt atcaatttca ttaagcatga cataaacttc ttcaatttca
     8641 gaaagctcgg cctgaacact ataagcttca acacctaaag cttttacttc ttcctcaact
     8701 tttttggtat tctgcagatt acggctgtgc aatatcaaat tgcagccctg ttttgccata
     8761 aaaatagcta tctgataacc aattcctctg ctggcacctg taataagtgc ccatttgcct
     8821 ttaacatcaa ccataacatc tgctccttaa tctcttcagg attattaatt caataaaaga
     8881 ttttctatag tatataaaaa aaataatacc tcgtcaatat tttcaaataa ctatgaatgg
     8941 cttttaatta tacaaactgt tttttatcat taatccctga attttttaac agcattaaac
     9001 caaaggcaga ataaaaatat ataaaagata tctaagatta agatactgat actaaacagg
     9061 cttaatacgc cggaacctgc tcctacatta aataaggctt ctttaaattc ctgcccccaa
     9121 attttaatga catttgtggt cagtaattca taagtatagg gaaaaatttt taataaaccg
     9181 aaaatagcca gtaaataaac agccagagcc ataaaagtta aaagaatttc tttataactt
     9241 ttataaatca gttctcctaa ataagcttcc tgataccttt cttttatcct gtccataatt
     9301 ttttttacaa actttataat ggcaagcgct aaataataaa aggccatgat tgaaaaaagt
     9361 aaaagaaaaa tccagttttt tatttcaatc tgctttttga tttcataggc ttttatcata
     9421 tattgcattc ccaactggtc cggagtcatt gaagcgaatt cttccagagc caattcttca
     9481 ttttcttttt taaaagcaat ttgtatgctt tctgtttttt ttctgcttcc attttgaact
     9541 tctttagtcc ctatataaac atttcccaga ttttctctgt ggcttaaatg attaagaaaa
     9601 gggggtcggt tctcagtaat ccctacaatt ttaaaattct gccccagata atttaccgtt
     9661 aatccaataa catctgtaga cttaaacaac tggtaagcaa cctcttcttc caatacaatc
     9721 tcatagtcct catctgaacg cccggttaaa aagcttcctt ttaagactgg tgtatgaata
     9781 aatacaggat aattcttttc tgtttgtatg atgttcactt ttgcctgatt ataactgctt
     9841 tcaattaaac cggtccattc ggctgtatat aaaagttcca gataactgct gttatccctg
     9901 aaatgattga tttgttccgg acgaatgttt tctgttatcc caatgatttt attaaagcca
     9961 aagcttccat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggacgaa
    10081 tgttttctgt tatcccaatg attttattaa agccaaagct tccattgaaa tctttaacat
    10141 tttccataac taaacttgta aataaaaagc agagaagaag aaagagcaaa gagattaata
    10201 attttttacg gatttattta aaagcattat tcttcttccc cctcatacat ctttctcacc
    10261 cttatcccgt caaataaagg cttactgcta tatacaataa tctcttccga accgtctaaa
    10321 cctgatgaaa cagcacagtt tgtaaagtct tcctgctcta tttcaatata tttcttttca
    10381 gcataaaaga tttcacctaa agaagtttct ttacttttca ggacatagac aaaatttctt
    10441 cccatttctt ctccgacagc ttttttgggg ataatcattt tataatgagg actttgactg
    10501 gaataataaa aagttcccca ttcccagggt aagacttttt cttcttcata gcttatgacc
    10561 acttttcttt tggaaggatt tttttcatca ggccccagga ttttttcaat ttgaccttta
    10621 atgactttat tcccgaagga actaaacttt aaatccactt gttccccaat tcttaaatat
    10681 ttaacttcat caatattgac ttctgcaaca aattttgatt tttcagtagt attaataata
    10741 ctgcagaggg aattataagc atctgccaaa gccccttttc tgcaattgat tgcttgtata
    10801 atgccactac agggagctgt tatcctgcct cctgtatccg tatctttttc cagctctgag
    10861 cggattaatt ccatatcata ttctaacttt tttatttcca gtattctttc tttttcttcc
    10921 gcatcttttt ccaatttttt ttgttccagc tgactcaggc ttaactcgta attttgccgg
    10981 ctgtcttcca gtctgtactc agcttcctgg aggctttttg cactttctgc cccggattca
    11041 taaagggtct ttattttttc ataattttct ttttcccggt ccaaatttct ttttgcttct
    11101 tcaagacttt tttcataaat ggaaaggtcc actaaagcag gctggttttt tatttcctct
    11161 aactgaagct gtaacttttt taactggatt tctttctcca tagcagaaga atcctcagag
    11221 ccttctaata aaatgatggt atctcctgct tctacttgat cgccttcttc tactagaatt
    11281 tcttttatcc tcagattctt ttttgaataa acttctctaa tttcatcagg cagcacttgt
    11341 ccctctgccc tgatgctaaa aagcagattg cccccagaga ttttctcgct ttttacctga
    11401 ggcagagaaa gattaatgat ggttttcgag aaaaaggtca gcagcagcat aacagataaa
    11461 aacacaagga taattttact taatattttt ttcttaagca tcgctacagg atttaattct
    11521 tccatatata actcctttca gctcctgata tcagatttat tgaatatctg aatactgaat
    11581 tccccttacc aggtcctctt ccagatataa aaacagcaat aacactggca gcatataaat
    11641 aaaagaagct gcaaaagcaa tttctaaatc attttctcca ataacaggca gataagtaga
    11701 taaaggttgc ttttgaacat cgcttaaaaa taacaaaggc tgctctatca tattccagta
    11761 ttctataaaa gtcagaataa caagagctgc aatcccgtgt tttaccatag gcaaaacaat
    11821 ataaaagaaa atcctgacca aaccagcccc gtcaattctg gctgcatcaa tacaggaatc
    11881 gggaatgtaa gtcataaatt gccgcaataa aaagacacca aaagcagaaa atattcctgg
    11941 taaaataagg gataaaggat tatccagaag ccctaaataa tccattacta agtaattggg
    12001 aaccagggtc acctgaaaag gcatcagcat ggtaatgata tataagaaaa atattttttc
    12061 ccggtgagga aatttaagtt tagcaaaaac ataagctgcc atagagccca ctgcaacttg
    12121 tccgataaca agtggcagga caatttttaa tgagttccag aacatagaaa gataggtagc
    12181 ttgatttagc aagattcttt tatactgcct tattgaaaat atgtccggaa taaaatcaaa
    12241 gcttatgtat tggctgcttt tacccgtttt aacggcgata attctttcag taacttcctg
    12301 aggcatcata aaagaagctc caaagatata aagaattgga taaagaaaaa ataaagaaac
    12361 aactgccaga aagacggttt taattaactt ggcgctaaaa cgagatctta ccatgactat
    12421 ttccttccta gataccaatt ttttgactaa actttttatc cagcttaagg attatgtaaa
    12481 caaggccata aataaatatc gctgtcaaat aagctgctga agtcagcttt tgataatcca
    12541 gagtaaaaaa gacattattc atataatgct gcagcaaata aatgctctca tggggataaa
    12601 caccagcaat taaatagact tccctgaaaa ctttaaaaga actcagtaaa gacataatca
    12661 aaacaaaaaa gaagacaggt gttaagtata ccaaagtaat atttttaaaa atccagcatt
    12721 tccctgctcc ttccagagct gcctgttcat aatattcctt tggaatgtta tttagtccgc
    12781 ccagaaaaag aaccatattg tatcctgcat ttttctagat atacataaga acaatgacaa
    12841 taatggtcca ggaactgtta aaccagttga ctgtcggtaa agctgtccgg ctaagcagac
    12901 cattgaccat accccggttc tgaaaagcca tattccacaa taaggcaacc gatgccgttg
    12961 gaattgccag gggtaaaata aaagttgtct taaaaaaact cttaaaaaaa acatctccgt
    13021 ttaaaatcaa agccaatcct aaagaaataa aaacagagag aggtacgctg attacagtaa
    13081 aaactaaggt attcttaacc gccagcctga aaacagggtt attgattaaa tccatatagt
    13141 ttcttaaagc gacaaaagaa gctccaaaag aagagtcaaa aaaggaataa acaccagcca
    13201 caataaaagg aataatataa aaaactgaaa atccaagcag gctgggagct aaaaaaataa
    13261 aggttacaat aaagttttcc tgtttatttt tgagacagtt cttaaatttt tgcattacaa
    13321 acccccttta tttcaattaa aataacacac tttaatgaat aaaaaataaa gagttgttaa
    13381 caaactgtta aacaactcta aaaattttta aaataaaata gacttctcat catatttaat
    13441 aaccttacaa acaatttctt ctaaaatttt attttctttg ctcataacca gacaactgtc
    13501 tacgtcaaga tattcctttt tctaccttta gattcttttt actttattca tttaaataaa
    13561 tctcaacttt tctctgaatt gcctcagctg tttcttttgc agactgctgg ttgtccagga
    13621 atttcctgat ttcttcatcc ataatctgtt caatattaaa atccctgtaa tgataaaggc
    13681 ctaaatttcc ggctacttca ttaatttttt tataatagct gtcgatgttg cccagctcaa
    13741 tgccatagac ttcttttaaa aatccatttt ccggatcatt gtctttttct aaaagttttc
    13801 tttctctttc cattttattt tgccttgctt tttcatttac tggtaataca attaattttt
    13861 cctgagactg caattcttct gaaagaaggt attttaaaaa tttccaggct tcttcctgat
    13921 tttttgactg actggatatg ccaagggagc tatttatcgt aaatgtgtaa ccattatccc
    13981 tgtcatcatc tgaaaccctg aaaatttcaa actcttttcc tacggagcgt accatctctg
    14041 ttgttgaata tcggtaatca tatatttgtt cataagccag attagcgtca gccgctcctg
    14101 ttaaaagatg ctcagcaggg tctgaaatca tcgcctcatc tatcatttct tttcctaaat
    14161 ttaataaagc aatgacttta tctgtattaa agtaagcttt tttctttgaa taatcgatta
    14221 actgattatt attcatgaag attaagcttt tcataaaata ttcattgttg ataaaataac
    14281 cagcaaaaac aggttgattc gttgctttta aatttttaac agccgcttca aagtcctcat
    14341 actgacatat tccgttcttt acttttatcc cgccggaatc caaccagtct ttatttacaa
    14401 tatatgtatc aatattatag gacataggaa taaaatacaa tttgttattg acctgaatat
    14461 tcctgataat attttgattt aattcattga tattaaaatc tgaatcatta tttatccgtt
    14521 cagttaagtc aagcagcaaa ttcttatcaa tatattcata gtaaggcaaa taatccagaa
    14581 aaagaatatc tgaccctttg cctgccataa tctctgcaga tatctgtgtt acataatcat
    14641 ccagcttctt ttcatattca tcataagcct ttttcagagc ttcatcttcc ggaactttag
    14701 gaaaatttat ctcagcatca tattgattta actgaatttt aacgtttgga ttttcctttt
    14761 catattgcct tgcagccgtt tctaaataag cattataaga aggaattgtt atggtcaggg
    14821 tgactttatt gttatccgaa ctgattattt cttccagctt acgatttaac ttgactaatt
    14881 ttgtagtatc cgttttataa tcaagtaata ttgcgataat atctttatta tcattcatat
    14941 ccactgcaag catattaccc atagagtggt cgacattttt cagatcagac attcttaaaa
    15001 tataccctgc ttccctgcca tccgagctaa attgattgat tcctctgtca tcaaatacgt
    15061 ataagctttt atctttctct atatatttac atccaccaag ttcaccagcc tggatttcaa
    15121 aaatgacttc atttttctta ggatcaactt tttttatact gccattgtca ccagcaagcc
    15181 ataaattatt ttcttcatct gtttctaaaa aataaatatc attgtttaaa ttaaattcta
    15241 aaaacttttt accttctggc gtataagcta caactttttt ccctaaatat aaaagataat
    15301 aatttccatc tccatccaac tcaaagtcaa tctgatgaag ataatcattt tcctctataa
    15361 gatgagaacc taaataaatt tcctctaatt tgctgccagc taaatcatat ttgcaaattt
    15421 tttgataaac tttaggctga ctcccttctt ctacttcctt ttgccataaa acatataaat
    15481 aatcgttttt atctaaagta aataccatac caaataaata ataagatgta ttttttacag
    15541 tatcagggaa attttcatct ggtgggattt ccactagctt ttgattatct ttgctcataa
    15601 caaccagact tttattatcc atgttataaa gtatatatct cccatccgat gccattttta
    15661 tattctgtac tgcaaaatca aaatgaatca aattggttat atcttcgtct tcaacagtta
    15721 tttcaatatt ttcctgaggc tcaacaatca tattatgatt ttcattatcg gcagtttcat
    15781 ttcctttatt gctgctacag ccacaaatat tgattaagaa gataaaaata atgataaaag
    15841 ccagtatttt tttcattaag ctacctcctg ttttttcaaa agcataacat attcattaag
    15901 catcataacc taatcataac ctagctttta taacttttat aataaaagaa aaagagttgt
    15961 aaaaacttta tcagacaact ctaaaatctt tcaaaaaaat ttgaatatcc gtcccctggt
    16021 caatgtcgct ttctattttt atctcccacc tgtgtaagtc aattattttt tttacaatat
    16081 aaaggcctac accgctgcca ccctgttttc tggctctgga gcggtttacg gtataaaaat
    16141 tatcaaaaat atgttcctga gattcttctg gaatgccaat accgttatcg gcaacgccaa
    16201 gaattaaata gctatcctct ttttgggtat atatctttat aaatccattc tctttgccat
    16261 atctaatact attatccatc agattaatca tgacttcttt taagccgttt ctgtctgcca
    16321 atatttcaat gttttcgtca cactcattgt accattctat ttttttatcc ctgctgagcg
    16381 ctgaaatttg ctttataatt tcattaacaa aatcttttat agggatgtat tctttatcca
    16441 gcttgatttc ttccaaccgg ctggccgtta ataaagactg aaccaattta gaaagacggt
    16501 tgacctcttt agatatatct gccagatatt cctgataggt ctctatactg tcattttcgt
    16561 ctaaagattc gattagggcc tgaatagaag ccagaggtgt ttttaattcg tgagaaacat
    16621 tacttataaa agttctccgg ttgctttcta taaagtccag ttgtctgcac atctggttaa
    16681 aagtcttagc caaaattcct atttcatctt ttctgtcaat atttacctgt acctttaaat
    16741 caccttccaa aattctttta gaagcatcga tcagtttttt aacaggtttt tcaacataat
    16801 atcctgccca acaagataaa ataataatgg caatccaaat aatcaaagaa aactccagca
    16861 cttttttccc ataatcccat acattttcaa tgacatcctg gacatataca gagataagca
    16921 ccattccatc cctatagttt aattctctgt aataagttcc cacaggctga aaaacaggga
    16981 tcgtcatcat aagcatcttt ttaccgttag aataataata gcctgaagcc atagtttttg
    17041 tttcctgagt ctttctgatt tccttattgc tgattaattc tcccactaaa atatcttgac
    17101 tgtcaatgat gacctcccca tcatcattta aaattaaaat acggccagac atattctcct
    17161 cataacggtt taccaaatcc attaattcca ggctgtctcc ataatatgcc ctgaaagaag
    17221 caataatatt tccatttttt tggatattgt cttccaactg ttttaactct tttttatagt
    17281 aggtaaaaat taataaacta tcaacaataa cgcatgaaat aaataccata attccaaaca
    17341 ccattgtaaa acgaaaagaa agccgcttca tgcttaaatg acatccttcc tgaaataata
    17401 gccaactccc catttggttt ttaaataata aggattggca gggtcatcct caattttttg
    17461 ccttatttta cgaatatgaa catccactgt tctggtatca aaactcactt catgccatac
    17521 cagttcataa agcatttctc tgctaaaaac ttttcctctt tctttcatca ataaatataa
    17581 aatgtcaaat tccttagccg taagcggtat ttcttttcca tttaaataag ccgttcttga
    17641 agatgcacag acttttacag cacccacttc aatgacatcc tcttcttctt ttactaccga
    17701 catactgttt acccttcgaa ggattgcttt aatacgggca attaattcct tagtatgaaa
    17761 aggtttggtt acatagtcat cggcacccat ttcaagacca acaattttat caatataatc
    17821 atcctttgca gtcagcataa taatgggtac atccattgtc tgcctgattt ttttgcataa
    17881 agttattccg tcaatttccg gcagcattaa atccaataaa atacaatcaa tcttttcatt
    17941 attgataagc tctaaagctt ccttaccatc atatgctgta aaaacattat aaccctcttt
    18001 tgtcaggctc ttcttcagtc ctttgaccaa ggcctgttca tcatccacaa tcagaatatt
    18061 cataaacttc tcccctgtca atattgatgg aattctgttg aaaatagtat accatattca
    18121 aaaaatttat ccaatagtta gaaaattata tttttcctgc tgctcttttt aacaagagca
    18181 gcagcattta aaaaacctga tttcatcata aataaaaaca cttccatcaa agacaaaagt
    18241 gccataatca ataacgaaca gcatcattaa aataaggaaa gctggctgta ttgataccat
    18301 tgtttataag caaaaatgat atctttcatt ttataaagca tacctaaacg ttcacattct
    18361 ccttttaaca aataccaaag ctttttggct tttggagaag gacactgata agcattgcca
    18421 tattgctgga tgtatttttc tttaaacccc ggaaaatatt catctaatct attataatac
    18481 cattcccttt gattttgcct taaagttacg ccaaaagcag gatagataaa ctttgctccg
    18541 cttttgtggg caaactggat aattcccttt atattttctt cattgtcttc tataaaaggc
    18601 aataccggca tcattaaaat ccctgtaaaa atccctttat ccgataattc cttaatgcat
    18661 tcaaaacgtt ttgaagcaag ggcaacatta ggctcaataa tacggcataa atcatcatca
    18721 caagttgtaa tggttatttt tattaacact ggtgaatgtt cctgtattga ctttaaaagg
    18781 tcaatatctc ttcttaccag atcgcttttt gtggcaatag ccgctccaaa accatagcgg
    18841 tggattaatt ccagggctcc tcttgtcaaa gcatactttt tttcaaaagg attgtaaggg
    18901 tcactcatag cccccgttcc tacaacccct gtttttcttt tcgatttaag ttcctttgca
    18961 aggatttcca atgcattctt tttagccctg accttatcaa agttttctac tccatagcag
    19021 tcactgcggc tgtcgcaata aatacagcca tgacaacagc ccttataaat attcatatta
    19081 tagttgaccc caaaccaggg attattggta ctataggcag aaagaatatt ttttgcttcg
    19141 ataaaatcca tatcagatac ctttctcttc taaaaatttt ataaattttt tatcggacaa
    19201 aatttcattg gttacaaatt caccatcata aaagagactg taagttgtaa atggtgaggg
    19261 ggcgttttgg gcttcttctt tagattcata gcggataagg gtaaaatcca gtcccctttc
    19321 ccgggctatt ttttcaatga ttatcgcata cttttctgca tggggacact gattagaata
    19381 atataagaca aaacctttct tatgaatttt tcctgctctt gcataatctt tgaattttgg
    19441 ttcttttata ttttcatcaa aaggcaaata ataaagttca aaataaggat tagctttatc
    19501 cgatactata aaacctttat attttaaata ctttggatct gataaataag gccttttctt
    19561 atctgaagac aaaataacca gtcctactct tttcatggct tttgcatctt caatgcattc
    19621 tttaagcagt aaattggaat atccctgtcc tttatactgt cccgaaaccc aaagacaatt
    19681 aataaacata taaccgtctg catcaatagg acaccatgca ttttcagccg gtatgtactc
    19741 aataaaaacc ttgcctctta catccagttt cttaaataca agaccttctg caaatctgtc
    19801 tttaagccag gctttttttg ctgcaacagc cggatctccc tttgtattcg aaatggcaca
    19861 gcaaatatgt tcctgttcga gattgtccat attcacacgg ataatattca atcttatctc
    19921 cccttccctt atcttattaa aataagcctg aagccgattg atgaattgta atttaagtat
    19981 ataaggctta acatgacaac aatatgtcat attacaaata tttttttaaa ccgtcttcaa
    20041 aatttttcat gatgattaaa cggattcttt caggctctaa gacttctgca taactcccat
    20101 aagacaggat gtaactgtaa agccaatccc cctccggaaa aacggttgaa ataataaaag
    20161 ttccatcgtt ttgcttttta ataagttctt catcaaattc atcgtagacc cggtaagcca
    20221 ttttggcgtc tatttttaat acaagtttta ctgctttgtc atcgtccatc ttcaacactt
    20281 gccctgtttc cgcaggaatt tttctttcaa attgctcctc taaaagattt aaatttttca
    20341 ttcttgtcag tttaaaaaat ctaaagtcat ttctgcttct gcaaaaagca taaaggtacc
    20401 agctttgtcc cttaaaaaac aattttaaag gttcaaccag cctgattgtt ttttctccgg
    20461 aagaactaaa ataatcaaag ctcatcagct ttttattaaa aattccttct ttaatttgct
    20521 taaatttttc cttatccctc ttagagttgc cccagccaga aaaatccaca tcaatccaat
    20581 gaatactctc ctgattaaaa atagcactta atttattaag aatagattcc atattgggtg
    20641 tattaatagc atttaaacct tgaaggctca taaggagttc tttttgctct tcctcagaaa
    20701 acaaggattg ctttagcaca taattttcaa tcaggctaat gcctccacct tttcctttac
    20761 tcgtatatat aggaatacct gctgaagaaa gggtatccag gtcacggtat atggttcttg
    20821 ttgaaacctc aaaatgttct gccaactctt tagcggttac agtttttttc ctcagtaata
    20881 tataaaccat ttcaaataac ctgttaatct gcataaacat cacctgatta tattttatca
    20941 tatttatatg acaagactat gtcatactta aaatcaaatg aaaaagttta aagtttaaaa
    21001 ttgatcctat aaaataaagc tgccagttca cttatttaaa aattagctgg cagctgttac
    21061 tcaaataaaa aagtatatgg taaaaaataa taatatgctt cctgcaagaa caaaaagatg
    21121 ccatacaaaa tgatgatatt taaaccagtc aaatacaaaa aataaaacac caatgctgta
    21181 acacaaacct ccagaaagca ataagacaaa gcctcctaaa gatacggtct tataaattgt
    21241 ggcagcaaaa aaaataatca tccagcccat aatcaaaaaa attaagacat gaatcttcgt
    21301 gtatttttca atccagagta ctttaaaaat aacacctaaa atggcaagaa gccattgaat
    21361 acagcaaata acgataccta taatgccgcc aatggcaaaa aagtaaaaag gggtataggt
    21421 tcctgcgatt aaaatataaa tcgaagaatg atctaaacgt tcaaaaatat tctttgcttt
    21481 tccttctgga aatgcatgat aaaacatgga attggtataa agaaaaataa gagaaacaga
    21541 ataaatgagc atacttatga tgtataaagg attccctctt aaggcccctt taatcaccaa
    21601 taaaaccagg gcagcaattc ctaaaagcaa gcctgctcca tgagaaatgg aatgggcaat
    21661 ttcttctccc aggctaaagt gctttcttac aataatatcc ttaaacttaa acattttgac
    21721 acctcatgac tataataaat atcctttttt agcactatat atgccaataa tgattcctct
    21781 ttgtctttaa ctggtttaaa tccttcctga ctctttcatc cttaaaatta aactttatca
    21841 taaacattac cttctttttt attaggattt tagcaactcc agaaattaaa taattgccaa
    21901 taaattataa ataggctgat ataacagctt tcaacaacag ttgcaatata gcgtctttta
    21961 ctgggtttcg ttaaattctt tttatcccat tgttttatca taataaccgt attgattaaa
    22021 ctgatgacaa cagcaattgc caaaaccatg cattgtatca ttactgaaag ccttgtccat
    22081 gtcactggcg ctaagattaa atagtaaaat gtaaaggcca caatggcatt ttcaatggtc
    22141 ataatgatat agattctatg taatttttta tggcttatgc tttcttcttt tcttccaatc
    22201 tttctgataa caaaaaataa gcccttaaat aaaatcttaa a
//
LOCUS       DAOSUD010000034        21622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1588_length_21622_cov_28.192934, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000034 DAOSUD010000000
VERSION     DAOSUD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 21622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21622
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_21622_cov_28.192934"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1505)
                     /gene="asnS"
                     /locus_tag="PLG49_05895"
     CDS             complement(114..1505)
                     /gene="asnS"
                     /locus_tag="PLG49_05895"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOQ16890.1"
                     /translation="MKFDTVKELYTNTDDYADSTVTVGGWVRTIRSSKTFGFIELNDG
                     TFFKNIQIVFEDNLDNYIEITKLNVGAALIVEGTLVKTPEAKQPFEVKATKIVIEGKS
                     TPEYPLQKKRHSFEYLRTIAHLRPRTNTFSAVFRVRSLAAYAIHQFFQERGFVYVHTP
                     IITSSDAEGAGQMFRVSTLDLNNLPKDEEGKVDFREDFFAKETHLTVSGQLSAEAYAM
                     AFRKVYTFGPTFRAENSNTARHAAEFWMVEPEIAFADLNDIMELSEDMLKYIIQFVME
                     HAPAEMEFFNNFIEKDLFDKLVNIVNSDFAHITYTEAIELLKKADVQFDYPVEWGCDL
                     QTEHERYITEKIFNKPVFVTDYPKDIKAFYMRQNDDGKTVAAMDLLVPGVGEIIGGSQ
                     REERYDVLEQRIKELGMKEEEYKWYLDTRKYGGTRHAGFGLGFERAIMYITGMTNIRD
                     VISFPRTVKNADF"
     gene            complement(1580..2590)
                     /gene="asnA"
                     /locus_tag="PLG49_05900"
     CDS             complement(1580..2590)
                     /gene="asnA"
                     /locus_tag="PLG49_05900"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOQ16891.1"
                     /translation="MSDLIIPKNYHSYLSVRETVKAIKALKDFFEQALAKDLNLARVS
                     APLFVKSKTGLNDNLSGIEKAVSFEVKGIGGETVEIVHSLAKWKRMALKKYNFAVGEG
                     LYTDMNAIRKDEDMDNLHSIYVDQWDWEKVIRKEDRNIETLKEIVERIYGVFQKTEKL
                     ISDLYPDIKPILPEKITFITTQELEDRYPDLSPKQREDAVTKEYGAVFLIGIGDKLNS
                     GQKHDSRSPDYDDWRLNGDILFWYPVLEKTMEMSSMGIRVDEDALLRQLQIAGCEERK
                     ELEFHQQLLKGELPYTVGGGIGQSRLCMFFLRKAHIGEVQTSVWPDDMIKICKEANIF
                     LL"
     gene            complement(2685..4190)
                     /locus_tag="PLG49_05905"
     CDS             complement(2685..4190)
                     /locus_tag="PLG49_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ16892.1"
                     /translation="MIKEKSNSYWITSTKRTAYPELTEEISVDAAIIGGGMAGISCAY
                     YLKNEGLKVCIIDRKRILEGVTGHTTAKITAQHHLIYDSLISDFGIEKASQYAEANLW
                     AIEEIDRIISENHINCDFIRQPAFVYTQSDEYIEKIEKELRAAEQIGIHASFENELPL
                     PFSVKAAIKFDNQAQFHPRKFLLSLAEKLVNEGVLIYENTRMKDISDENPYRVITDKG
                     EIKAKYVIIASHFPVYDHLAFYFARMYQSRTYALGLKIKEKFPGGMYLSAESPSRSLR
                     AQPTEEGELVLLVGEEHKTGQPQETKSYYEALKKFACENYDVESIPYHWSTQDCMPLD
                     NIPYIGRIKDGVENMFVATGFKKWGMTHSIISGLIIKDLIINRDNSWIELYNPSRFTL
                     KQSFKKFFEVNLDVAKELVSGKLEIPENSLKDLKNEEGVVIDIEGRKVGAYKDNKGEV
                     YLVDTTCSHLGCELKWNDAEKSWDCPCHGSRFTYTGEVIQGPAVKNLQRLN"
     gene            complement(4318..5058)
                     /gene="nagB"
                     /locus_tag="PLG49_05910"
     CDS             complement(4318..5058)
                     /gene="nagB"
                     /locus_tag="PLG49_05910"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOQ16893.1"
                     /translation="MQIYIEENYEKMSKQAAYLLASQIRLKSQSVIGLATGGTPVGMY
                     KELVRMHKEEGLDFSQITSFNLDEYCPISRDNPQSYYYYMMDNLFNHVNIKPDKINIP
                     NGNAEDIEAECKSYEKKIMDAGGIDFQVLGIGNNGHIGFNEPDDKFEAFTHLVHLDEG
                     TIQANSRFFNSIDEVPRRAISMGIKTIMLAKKIMLLANGKNKAPIIKETIFGEITPKV
                     PASVLQLHPDVTFILDEEAASEIIPLLK"
     gene            complement(5069..6211)
                     /gene="nagA"
                     /locus_tag="PLG49_05915"
     CDS             complement(5069..6211)
                     /gene="nagA"
                     /locus_tag="PLG49_05915"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOQ16894.1"
                     /translation="MKCLYNGKLIIKGRLYEKKAILFDEKIIDIINEEEIHHISNLEK
                     IDARGCYISPGFMDIHIHGYDGFDTMDATEEALETISRGIAGNGVTSFLPTTMSMPKE
                     NIIQALNTIRKIREKGTSGAEILGAHVEGPFINKKFHGAQKKEYIIPFDCDIINDYKD
                     VISLMTIAPELEGALDFIKEVKRKSNIVLSMGHSAATFEEALEGIAYGICHVTHLFNA
                     MSGLHHRNPGVVGAALTTNVSCEIIADEIHIHPDLFSVILKAKGLDGIVLVTDCMSAG
                     GKEDGVYELGGQKVFVKDKEARLEDNTLAGSVLKLNHAIYNLYKRTNYSIPQLVQLVS
                     INPARVLGLESVKGTLDIGKDADITVFDEKINILMTIGKGKIIYAI"
     gene            complement(6367..7251)
                     /locus_tag="PLG49_05920"
     CDS             complement(6367..7251)
                     /locus_tag="PLG49_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ16895.1"
                     /translation="MKILALDIGGTAIKIGLINKNGEIIERREVPTLAKEGGEALMNK
                     ILKLIGEYGCIDRIAVSASGQVDPQEGKIIFATDNIPGWTGMEIKKRIEERYNIATVV
                     ENDVNAAAIGEAYYGAAEGFENFLCLTYGTGIGGAIVEKGEIYRGCFNSAGEFGHIIT
                     HANGKDCTCGSKGCYEVYASTTALVREAKEKLAIENVNGKKIFQYLSQGNDEVKEIVD
                     NWLNEIIIGLVSFVHIFNPSLIVLGGGIMSQNYIIEYLRKHLAKSVMPNFRKVKLVQA
                     RLGNDAGILGAAHIAMKL"
     gene            complement(7279..8136)
                     /locus_tag="PLG49_05925"
     CDS             complement(7279..8136)
                     /locus_tag="PLG49_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOQ16896.1"
                     /translation="MPNNDFLLSIQSHYHQFTKAEKKVADYVLRNPKKVLFMSITDLA
                     DACDVGDTSVFRFCRTMKLRGYQEFKMRLSLSIKTDNKETEELESDVTPDDSFATLAQ
                     KVLKSNIDAINETYSLLNPEEFIRCMDYFEAAENVYFFGVGSSMLTAMEAMDKFLRIT
                     PKVHCIPDSHMQSMVASMLTEKDLAIIISYSGSTKDSIHVAKTAKQAGAKVICISRFI
                     KSPLTGYADITILCGANESPLQGGSTSAKMCLLYIIDLMYMEYYRRNYDISFENNQKT
                     MSSVLEKLY"
     gene            complement(8149..8838)
                     /locus_tag="PLG49_05930"
     CDS             complement(8149..8838)
                     /locus_tag="PLG49_05930"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HOQ16897.1"
                     /translation="MSDSVLNRIKGGLIVSCQALNDEPLHSSYIMSRMAYAAMLGGAV
                     GIRANTVEDIQEIKKLVHLPIIGIIKRIYEDSDVYITPTLEEVDALVNCGVEIIAMDA
                     TFHLRPKSQSIDEFFTEVRKKYPNQLFMADCATYEEGMHAVQLGFDIVATTLCGYTSE
                     TKDVKLPNYDLMRKLVRDSGKPVIAEGGIWSPEQLKMAFETGVFAAVVGTAITRPMDI
                     TKRFVEAIKNE"
     gene            complement(9173..10084)
                     /locus_tag="PLG49_05935"
     CDS             complement(9173..10084)
                     /locus_tag="PLG49_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOQ16898.1"
                     /translation="MSRLDKYRGIFPAFYACYDDNGDVSPERTRLFTQYLIDKGVKGL
                     YVCGSSGECIYQNVEERKIILENVMEVAKGKISIIAHIAAPSTRDSIELAKHAEKCGV
                     DAVAAIPPIYFRLPEYAIEKYWNDMIDATDLDFVIYNIPGTTGYALTLDLFKRMIKNK
                     KVIGIKNSSMPTQDIQIFKAAGGEDFIVFNGPDEQFVAGRAIGADGGIGGTYAVMPEL
                     FIKADELIRSGDFVTAKEIQYEINKVIFAMLSCHGNLYSVAKEILRIQGIDIGEARLP
                     LPRFTEDDLPTIHQCVEIIEKAKKKFC"
     gene            complement(10077..11066)
                     /locus_tag="PLG49_05940"
     CDS             complement(10077..11066)
                     /locus_tag="PLG49_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ16899.1"
                     /translation="MSKKVWVNIDPQKVDVSYLEKRLKDSGFEVIYKVVLADDEAGTI
                     EMGNSVDAVVSVFERWNERTLPAVRGHLKFIQRYGAGIDNINLKAATEAGIPVANVPG
                     ANSAAVAEIALLHILNLGRRFKTVIDDTKKGLWPCAITGNELDGKVVGLVGFGNIAKQ
                     LARMLSGFDVKILVYDSYAQADTNQYDVKVVHSIEEIFLQSDIVSLHVPLNDETRGMI
                     DKRLLNLMKPSAYLVNTCRGAVINEADLIEALREKKIRGAGLDVLSLEPPSLDNPLLS
                     MDNVFVTSHMGAETAESGLRSQKIMADTIEAFFSGKIPANLMNKEIVKEGYHE"
     gene            complement(11104..12477)
                     /locus_tag="PLG49_05945"
     CDS             complement(11104..12477)
                     /locus_tag="PLG49_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ16900.1"
                     /translation="MKKRLVSILLMVALLVSLSACGGGKDNAGQTGENTGGSNTSQGE
                     SKTQGKQLTVWLEKVFSDDANAELQKRVQQFADESGIDVKFELLAATDFVPKLNAAIE
                     AGTNVPDVTLTSGAITRVLNYYPNIPNLDVTGLVDEIHANRPYFESIREGSKIEGVNY
                     FVPFYSSSTLMFIRKDKLEEKGITKYPTTWEEVFEVAEAVSDPKNGFYGLGIGCGPTD
                     EDCENTFRMIMWNNGAYVFNEDGTAAINSEKAKELIKKYAEMYGKEIIPPSASTWDPG
                     GNNSTYLMGESAIVFNAPTLYNALKGDEAYKEILENTVALAPPAGTDNSVVMEFISGF
                     AIMNTCKDVESAESFIKYMLDAEWYDEYFKITAPVFAPIFQDVENDEFWAEGINQQVV
                     NYAKNASGYYGYPVKSIEGRVTAAKHYFTYPVAELLNSVVTGTKTVDEAVEKMEADIK
                     QISSEIK"
     gene            complement(12597..13424)
                     /locus_tag="PLG49_05950"
     CDS             complement(12597..13424)
                     /locus_tag="PLG49_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ16901.1"
                     /translation="MQNKLVTILHRITVALFLIIALFPIYWMLNTSLKTQGEVYSKIP
                     TFLPKNPTLNNFKYLIFETPFMNALKNSFLVALVVTVLSIIIAYPTAYSISRLKFKGR
                     NTFAKVILFCYLLPSSVLYIPLYIFVSRVGLSDNIMGLMLIYPTFTLPYVAWILIPHI
                     SSIPYDIEEAALVDGCSKFKIMYQIIFPLTIPGVISTAIFAFAMCWGEYLYALVNITD
                     TQAKTFPLIIAGLIYGDLYPWGQIMAGSIIGCIPILIIYIASSRFLTGGATAGGVKG"
     gene            complement(13439..14356)
                     /locus_tag="PLG49_05955"
     CDS             complement(13439..14356)
                     /locus_tag="PLG49_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ16902.1"
                     /translation="MARIRKFRQNNMERQLPGSKKSFPYMLNIPMFLYLACVLFMPMI
                     WGISLSFTDKAIGGEASFIGLGNYIKLINDKEYLLSLWKTLQFASISLLFKLLLGVLM
                     AVVLNVEFKGRNIARALLLIPWTLPNVVAVLNWKWLFASTGGIINHILKLVGIIDKDI
                     VWFGTAGLALATIIVANVWRGTPFFGISILAKLQTISKDYYEAADIDGAKVWQKFFYI
                     TLPSIMDTIFLTTLMSSIWTINEFETVWLLTGGGPNGQTQVMNVYSYKTAMASMQIGR
                     GIAVSVLAMPVFLLIISLITKKSLADEKM"
     gene            complement(14598..16259)
                     /locus_tag="PLG49_05960"
     CDS             complement(14598..16259)
                     /locus_tag="PLG49_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOQ16903.1"
                     /translation="MESNKKQINEDHSNFIHDIIDKDLAEGVVKKVHTRFPPEPNGYL
                     HIGHAKAIWLDFSTAEKYGGLCNLRFDDTNPTAEDVEYVESIKEDVRWLGYDWGDRLY
                     YASWYFDQLYEYAIELIKKGKAYVDDLTAEEIREYRGTLTEPGKESPYRNRSVEENLD
                     LFIRMKNGEFPDGSRVLRAKIDMASPNMNMRDPVIYRIAHVKHHNTGDKWCIYPMYDF
                     AHPLSDAIEGITHSICTLEFEDHRPLYNWFLEALEIENRPKQIEFARLEVSNMVTSKR
                     KLRSLVEEKIVDGWDDPRMPTISGLRRRGYTPEAIKDFCQKTGVAKNKGVVDISFLEH
                     CIREDLKLKKPRIMAVLDPLKVVITNYPEGQVEMLDVENNAENPEMGTRQVPFSRVIY
                     IEREDFMEDPPKKFFRLSPGKEVRLKNAYFIKCEKVIKDEKTGEIIELHCTYDPETKS
                     GSGFSGRKVKGTIHWVSAEHGVPAEVRLYDHLFIEDEQTGELVINPDSLKVLKNCLIE
                     PSIKEAKKGDQFQFLRHGYFCIDTKDTTDEKLVFNRIVSLKSSWK"
     gene            complement(16396..17124)
                     /locus_tag="PLG49_05965"
     CDS             complement(16396..17124)
                     /locus_tag="PLG49_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ16904.1"
                     /translation="MIRKIVHIDEDKCNGCGLCVDACHEGAIELIDGKARLVSDEYCD
                     GLGDCLPACPVGAIEIIERESQAYNEELVNQKMAEREKLKKAQMPCGCPGTMAKTIQR
                     KPAVVKEKNEVKDEAKVELQSQLMQWPVQLSLINPDATYLDHPHWLIAADCTAYAYGN
                     FHEDFIKNHITMIGCPKLDDVNYYTEKLTEILLKHDPQSITVVRMEVPCCTGIAAAVK
                     KAMLNAEKIVNYREVIISTSGEIK"
     gene            17277..17978
                     /locus_tag="PLG49_05970"
     CDS             17277..17978
                     /locus_tag="PLG49_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOQ16905.1"
                     /translation="MNSDFSKGFKAGVPIALGYFPVSFTFGLIAVNGGIPVWLAIFIS
                     FSNLTSAGQFAGTNLIIANAPIIEIALTTFVINIRYMLMSLSLSQKIKSNMTLLYRSI
                     IAFGITDETFALASLEKNELTFSYMIGLISGPFAGWVLGTAAGAVTCSVLPEQVQSAM
                     GIALYAMFIALVVPPSKKSKAPLIVTIIGIFISSILKWLPYINQISSGFRIIIATIVA
                     SAIGALLFPKGEEEL"
     gene            17975..18277
                     /locus_tag="PLG49_05975"
     CDS             17975..18277
                     /locus_tag="PLG49_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOQ16906.1"
                     /translation="MSYAFTAVIVMAIVTYIPRVLPIAIFRKKIKSKFILSFLSYVPF
                     AVLAAMTFPDILYSTNHLSSALAGMITALILSYFEKGLMTVALCSILVVYLWEIFI"
     gene            complement(18392..20242)
                     /locus_tag="PLG49_05980"
     CDS             complement(18392..20242)
                     /locus_tag="PLG49_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOQ16907.1"
                     /translation="MGKIKIFIRNFMIFTLLLSLSFSSISLSYASNQATYVKNAESLK
                     ALGLFLGTDKGFELDRKSTRAEAAAMLVRLLGAEKTAAEEYKSKPHPFADVPAWASPA
                     VSYLYNKGLTFGISGNLYGAKDEITPEQYMTFLLRALGYSDRDGDFHLNNVLDFAHHI
                     GMINDSELSLLKAKEKTGVLRDYIVMMSYNCLFTKMKNENKSLLIHLAEKGVISNNKL
                     KAAARIDSQLKQLLMEEAYLDENNEGNKIENSIEGEVRGVWISYLELQNIFPNKTEAQ
                     FKESIRKMFRNIKDAGLNTVFVQVRPFGDAIYPSSYFPWSYIISGTEGVNPYYDPLEI
                     MVNEAHLQGLKIEAWINPYRIRNNNKPLSADNKALEWLNDGSNRVIKINSGIYYNPAS
                     KDVQNLIVKGIEEIIQNYDVDGIHFDDYFYPTTDMSFDAADYQKYLQEGGYLSQAGWR
                     RENVNQLVREVYKTIKAYDPDIKFGISPQGIISRNYNNQYIDVEKWLSNSGYIDYICP
                     QVYFGFNHSNYPYDKVVQEWNQLIKNSDVDLYIGLAAYKLGQEDAWAGNGKNEWIGTS
                     DILSRMIEEGRKSSNYKGFILFRYDSLWNPSQSIKDQVDAERIHLINCLD"
     gene            complement(20431..21534)
                     /locus_tag="PLG49_05985"
     CDS             complement(20431..21534)
                     /locus_tag="PLG49_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HOQ16908.1"
                     /translation="MQEFQYLMPTKILFGQGVIKKNASLFKEYGKKALIVTGKHSAKK
                     NGSLDDILEVLKRNGIAYSIFDEIEENPSLETVEKGADFGKKEKIDFIIAIGGGSPLD
                     AAKAINLLIANEDSSKEDLYILKPMKTLPGIAVPTTAGTGSETTPYAILTNHQKKTKI
                     NIVHKVFFDLALLDPEYMMAMPEAITINTALDALSHLVEGYLTVKANLFSDIWAEKGL
                     NLFSECMDALKKKNFTYLLREKLLLVSTIAGMVIAQTGTSLPHGMGYALTYFHNIAHG
                     RANALLMKAFLEIHPHRERVDRILSCLGLNNLEELGDFLDSLLGEKEKVTKDEIIAYA
                     QDMTFNDLKLKNHPAKVSLEDIYQIYEKSLNLQ"
BASE COUNT         6596 a         4405 c         3042 g         7579 t
ORIGIN      
        1 tggtttcaat caattcatct tctgaataga ttgaaaccag tctgattttt ataatgtttt
       61 ctcatcaatt taagccgata aacgctttta ttatataata actttttatt atcttaaaag
      121 tcagcatttt taacggttct tgggaaagaa atgacatctc tgatattggt catgcctgta
      181 atgtacatta ttgccctctc aaacccaaga ccaaaacctg catgtctggt acctccatat
      241 tttcttgtat ccagatacca cttatattct tcttctttca tacctaattc tttaattctt
      301 tgttcaagaa catcatatct ttcttctctt tgacttccac cgataatttc tcctactccg
      361 ggaaccaata agtccatggc agcaacggtt tttccatcat cattttgtct catataaaaa
      421 gctttaatat cttttggata gtctgttaca aatacaggtt tattaaatat cttttccgtg
      481 atatatcttt catgttctgt ttgaagatca caaccccatt caaccggata atcaaactga
      541 acatctgcct tctttaataa ttcgatagct tccgtataag taatatgggc aaaatctgaa
      601 ttaacaatat ttactaattt atcaaataaa tctttttcaa taaagttatt aaagaactcc
      661 atttctgccg gagcatgctc cataacaaat tgaataatgt acttgagcat atcctcggat
      721 aattccataa tatcatttaa atctgcaaaa gctatttccg gttctaccat ccagaattct
      781 gcggcatgtc ttgcagtgtt tgaattttct gcccggaatg ttggtccaaa agtatatact
      841 tttctaaaag ccatcgcata agcttccgct gaaagctggc cactaacggt cagatgcgtt
      901 tctttagcaa agaagtcctc tctaaaatct actttacctt cttcatcttt aggaaggttg
      961 tttaaatcta aggttgaaac cctaaacatt tgtcctgcac cttcggcatc acttgaagta
     1021 ataataggtg tatgtacata aacaaatcct ctttcctgga aaaactggtg aatcgcataa
     1081 gctgctaagg atcttactct aaatactgca gaaaaagtat tggttctggg acgaagatga
     1141 gcaattgttc ttaaatattc aaaagaatgt ctcttttttt gtagcggata ctcaggtgtt
     1201 gatttgcctt caatgacaat ctttgttgct tttacttcaa atggttgttt tgcttcaggc
     1261 gttttaacaa gtgttccttc tacaatcagg gcagcaccca cgtttaattt tgtaatctct
     1321 atataattat ctaaattgtc ctcaaataca atttgaatat ttttaaaaaa tgtaccgtca
     1381 ttgagttcaa taaaaccaaa ggtcttcgaa ctacgtattg ttcttaccca gccacctaca
     1441 gttaccgtac tgtctgcata atcatctgtg ttagtataaa gctctttaac ggtatcaaat
     1501 ttcatatgaa ctactccttc ctgaaaatta atcataaaaa tttatattat catcgtcaat
     1561 ttcaaagttt ttctttattt tataataaaa agatatttgc ttctttacaa atttttatca
     1621 tatcatctgg ccatactgaa gtttgcacct ctccaatgtg ggcttttctt aaaaagaaca
     1681 tgcataatct ggattgccct atgccgcctc caaccgtata aggcaactct cccttaagca
     1741 gttgttggtg aaattccaat tcttttcttt cctcacaacc agcaatttgc aattgcctga
     1801 gaagggcatc ttcatccacc cggataccca ttgaagacat ttccattgtc ttttctaaaa
     1861 caggatacca aaaaagaatg tctccgttca atctccagtc atcataatcc ggagaacggc
     1921 tgtcatgttt ttggccagaa tttaatttat ctccgattcc tattaaaaag actgctccat
     1981 attctttagt caccgcatct tcccgttgtt tcggagacaa atcaggatac ctgtcttcta
     2041 attcctgagt tgtaataaaa gtaatttttt caggtaaaat cggtttaata tcaggatata
     2101 agtctgaaat aagtttttct gtcttttgaa atacaccata aattctttcg acaatttctt
     2161 ttaaggtctc tatatttcta tcttcttttc ttataacttt ttcccaatcc cactgatcca
     2221 catagattga atgaagatta tccatatctt catcttttct tatggcattc atatctgtat
     2281 aaagaccttc accaacagca aaattatatt ttttgagagc cattcttttc catttagcta
     2341 aggaatgaac aatctcaacc gtttctccgc ctattccttt tacttcaaat gaaaccgctt
     2401 tttcaatacc actcagattg tcatttaatc cggttttact ttttacaaat aggggtgcag
     2461 atactctagc taaatttaaa tcttttgcca gtgcctgttc aaaaaaatct tttaatgctt
     2521 tgatcgcttt tacggtttca cgcaccgaca aataagaatg ataatttttt ggtatgatta
     2581 aatcactcat agatttcccc ctaatttcta tatttctatt aattatttaa tttcaacatt
     2641 atatcacatt attatggcgt tagtaaaata aatatataaa gtttttaatt taacctttgc
     2701 aaatttttta ctgcaggtcc ctggatcact tctcctgtgt aagtaaatct ggaaccatgg
     2761 caagggcagt cccatgactt ttctgcatca ttccatttta attcgcatcc taaatgagaa
     2821 caagttgtat ccactaaata tacttctcct ttattatctt tataggcacc cacttttctg
     2881 ccttcaatat caatgacaac gccttcttca ttttttaaat cctttaagct attttcaggt
     2941 atttctaatt tacccgaaac caattccttg gcaacatcca aattgacctc aaaaaacttt
     3001 ttaaaagact gtttcaaagt aaatctcgaa ggattatata attcaatcca ggaattgtcc
     3061 cggttaataa tcaaatcttt tattatcaga ccagatatga tgctgtgtgt catgccccat
     3121 tttttaaatc ctgtagcaac aaacatgttt tccacaccat ccttaatgcg tcctatataa
     3181 gggatattat ccaggggcat acaatcttga gtagaccagt gatatggaat tgattctaca
     3241 tcataatttt cacaggcaaa cttttttaaa gcctcataat agcttttcgt ttcctgaggc
     3301 tgaccggttt tatgttcttc tcctaccaaa agaacaagct ctccttcttc agtaggctgg
     3361 gctcgcaaag acctgctggg gctttcagca gaaagataca tgcccccagg aaatttttct
     3421 tttattttta gtcctaaggc ataagttctt gactgataca ttctggcaaa ataaaaagcc
     3481 aaatgatcat agactggaaa atgagaagca ataattacat atttggcttt tatctctcct
     3541 ttatccgtaa taaccctata aggattttca tcggatatgt ccttcatcct ggtattttca
     3601 tagattaaaa cgccctcatt gacaagtttt tctgccaatg aaagcaaaaa ctttctagga
     3661 tgaaattgag cttgattatc gaattttatt gctgccttaa cagaaaaggg taaaggcaat
     3721 tcattttcaa aagaagcatg aatccctatt tgttctgcag cccttaattc cttttctatc
     3781 ttttcgatat attcatcaga ctgggtataa acaaatgccg gctgccttat aaaatcacaa
     3841 ttgatgtggt tttcactgat tatccgatca atttcttcta tagcccataa attagcctct
     3901 gcatactggc tggccttttc gataccaaaa tcactaataa gagaatcata tatcaaatga
     3961 tgctgagccg taatttttgc agtagtatgt cccgttaccc cttctaagat tctttttctg
     4021 tcaataatac aaacttttaa accttcattt tttaagtagt aagcacaact aattcctgcc
     4081 atgcctccgc ctataatggc tgcatcaaca gatatctctt cagtaagttc aggataagcc
     4141 gttctttttg tagaagtaat ccaataagaa ttagattttt cttttatcat aaaaaatagc
     4201 tcctttgcat aaataatcaa ttttattttg tacaggaact atttttctat gtattcattt
     4261 aaaatattta atgttataaa actattaatt ataaaaaata ggagccctta gctgctccta
     4321 tttaagtaat ggaataattt ccgatgctgc ttcttcgtca agaataaaag taacatctgg
     4381 atgaagctgc aaaacagaag ccggaacctt tggtgtaatc tcaccaaaaa tggtttcctt
     4441 aataattggt gctttattct ttccattggc taacaacata atcttctttg ctaacataat
     4501 tgtttttatg cccatgctta ttgcccttct tggtacttca tcaatggaat tgaaaaatct
     4561 tgaattagct tgaatagtcc cttcatctaa atgaactaga tgtgtaaatg cttcaaattt
     4621 atcatctggt tcattaaagc caatatgacc gttgtttcca atccctaata cctgaaaatc
     4681 aatgccaccg gcatccataa tctttttctc atagctctta cattctgctt cgatatcttc
     4741 tgcatttcca ttaggaatat tgattttatc cggcttgata ttcacatgat tgaataaatt
     4801 atccatcata taataatagt aactttgtgg gttatctctt gaaatagggc aatattcatc
     4861 caaattaaaa ctggtaattt gagaaaagtc caagccttct tccttatgca tacgaaccag
     4921 ttctttatac ataccaaccg gagtccctcc agtagccaat ccaataacac tctgactttt
     4981 caggcgtatt tgacttgcta ataaataagc ggcttgttta ctcatttttt cataattttc
     5041 ttcaatataa atctgcatat tatttaattt atattgcata aattattttc ccctttccaa
     5101 tcgtcataag aatatttatc ttttcatcaa atacggtaat atctgcatct tttccaatat
     5161 caagtgttcc ttttacagat tcaagtccta aaactctggc tggatttatg ctaaccagtt
     5221 gaactaactg agggatgctg taatttgtac gtttataaag attatatatg gcatgattta
     5281 atttaaggac actccccgct aaagtattat cttctaatct tgcttcttta tctttaacaa
     5341 ataccttctg gcctcccaat tcatacacgc catcttcttt gccgcccgca gacatgcaat
     5401 ccgttaccag tacaatacca tctaaacctt ttgctttaag tattacagaa aatagatccg
     5461 gatgaatatg aatttcatcc gctataattt cgcaactgac atttgtggtt aatgcagcac
     5521 caaccacgcc gggattgcga tgatgaaggc cagacatagc attaaacaaa tgtgttacat
     5581 gacatatgcc ataagcgatt ccctccaggg cttcttcaaa agtagcagcg gaatgtccca
     5641 ttgataatac aatgttgctt tttcttttaa cctctttaat aaaatctaaa gctccctcta
     5701 gttcaggtgc gattgtcatt aatgatataa catctttata atcgttaatt atatcacagt
     5761 caaaaggaat aatgtactct tttttttgtg cgccatgaaa ttttttattg atgaaaggac
     5821 cctcaacatg agctcctaaa atctcagctc cagaagtgcc cttttctctt atttttctta
     5881 tggtgttcaa agcctgaatt atattttctt taggcatcga catcgtcgtc ggaagaaaag
     5941 aggttacgcc gtttcctgct attccccggc ttatggtttc taaggcttct tctgttgcat
     6001 ccatggtatc aaaaccatca taaccatgaa tatgtatatc cataaaacct ggagaaatat
     6061 agcaacccct ggcatctatt ttttccaggt tactgatatg atgaatttct tcttcattaa
     6121 taatatcaat aattttttca tcgaaaagaa ttgctttttt ctcatataat ctgcctttaa
     6181 tgattaactt tccattatat aaacacttca tatctttacc tcctaatgaa cattttgttt
     6241 agtttttatt ataatctttt tcaacaaaaa taatccttcc accagaatat acttttatta
     6301 aaattaggat aataaagtaa attgaagcag ttaacaaaaa aaggacatga aaaatcatat
     6361 ccttctctaa agcttcattg ctatatgcgc agcccctaat atccctgcat cattacccaa
     6421 acgggcttgt actaatttta ccttcctgaa attgggcata acggatttag ctaaatgttt
     6481 acggagatat tctatgatat aattttgtga cataattccc cctcctaaaa caattaaaga
     6541 aggattaaat atatgaacaa aacttactaa gccaataatg atctcattca accaattatc
     6601 aacaatttct tttacctcat cgttaccttg gctaagatac tgaaagattt tttttccgtt
     6661 tacattctct atagctaatt tttctttagc ctctctaaca agagcagttg tagaggcata
     6721 aacttcataa catcctttcg acccacaagt acaatctttt ccatttgcat gggtaataat
     6781 atgcccaaat tctccggcag aattaaagca tcctctgtaa atttcccctt tttctacaat
     6841 tgcaccacca atgcctgttc cataagtcag gcataaaaaa ttttcaaacc cttcagcagc
     6901 tccataatat gcttcaccaa tagccgctgc gttgacatca ttttctacaa ctgtcgctat
     6961 attatatctt tcttctattc gctttttaat ttccattcct gtccatccag gaatattatc
     7021 cgttgcaaaa atgatttttc cttcctgtgg atccacttgt cctgaagcac taaccgctat
     7081 cctgtcaatg caaccgtatt ctccaataag ctttaaaatt ttattcatta aagcctcccc
     7141 accttctttt gctaaggtag gaacttctct tctttcaatg atttctccat ttttattgat
     7201 aagaccaatt ttaatagctg ttccaccaat gtccaaagct aatatcttca tttttcacct
     7261 ctaaaatcac tattgatctt agtataattt ttctaaaaca gaactcatcg tcttctgatt
     7321 gttttcaaag ctaatatcat agttcctgcg ataatactcc atatacatta aatctataat
     7381 atacaaaaga cacattttag ctgaagttga accgccttgt aaagggcttt cattagcacc
     7441 gcaaagaatt gttatatctg cataacccgt cagcggggat tttataaatc tgctaatgca
     7501 tatgacctta gctcccgcct gttttgcagt ttttgcaaca tggatactgt ctttagttga
     7561 accagagtat gaaataataa tggccaaatc tttttctgta agcatacttg ctaccatgga
     7621 ctgcatatga gaatctggaa tacagtgaac ttttggtgtt atacgtaaaa atttatccat
     7681 agcttccata gccgtcagca ttgaagagcc aaccccgaaa aaatatacat tttctgctgc
     7741 ttcaaaatag tccatacaac gaataaactc ctcaggattt aaaagagaat aagtttcatt
     7801 gatagcatct atattggatt ttaaaacctt ttgcgctaaa gttgcaaaac tatcatctgg
     7861 cgtaacatcc gattccaact cttcggtttc tttattatct gtctttatac tcaatgaaag
     7921 acgcattttg aattcctggt aaccccgtaa tttcatcgta cggcaaaagc ggaatacact
     7981 ggtatcaccc acatcacacg catccgccag atcagttata gacatgaaca atactttttt
     8041 tggatttctt aatacataat cagctacctt tttttcagcc ttagtaaatt gatgataatg
     8101 ggattgtatg cttaatagaa aatcattatt tggcatattt tctactcctc actcattttt
     8161 aattgcttct acaaatcttt tggtaatatc cataggccgg gtaatagcag ttccaacaac
     8221 agcagcaaac actcctgttt caaaggccat ctttaattgc tccgggctcc atatgcctcc
     8281 ttcagcaata acaggtttcc cagaatctct taccagtttt ctcattaaat cataattggg
     8341 aagtttaaca tcttttgttt cagaagtata accacacaat gtagtcgcaa caatgtcaaa
     8401 tcccaattga actgcatgca tgccttcttc gtaagttgcg caatccgcca taaataattg
     8461 attgggatat ttttttctca cttctgtaaa aaattcatct atactttgtg atttggggcg
     8521 caaatgaaaa gttgcgtcca ttgcaataat ttctacgcca cagtttacaa gagcatctac
     8581 ttcttctaaa gtaggtgtaa tataaacatc actatcttca tatatcctct ttataattcc
     8641 aataatgggc aaatgaacaa gtttcttgat ctcctgaata tcttcaacgg tatttgctct
     8701 tattcctaca gcaccgccta acatggctgc ataggccatg cgggacataa tatatgaact
     8761 atgaagaggt tcatcattta aagcctggca ggaaacaatt aaacctcctt ttattctgtt
     8821 cagcacacta tcactcacca tatctcacct actatctaaa gactcctatg tccatctatt
     8881 taaatatata atatcatata aaaattgtat catatataac atttttttac ccttatcgat
     8941 ttattcttat ccatcaaaaa aatataataa ctgaataata agaatgagaa aggaagaacc
     9001 tttgtcttca ctttctcaat ctaaatattt tatcaagaag cttgccgtga taagaacatt
     9061 taaatagaat tcatatcata catgaattag atgcgaaaac actaattctc attttaataa
     9121 tgattattta ttcatccaaa ttttattgaa aatatttaag gctattttgt tattaacaaa
     9181 atttcttttt cgctttttcg atgatttcaa cacattgatg aatggttggt aaatcatctt
     9241 cggtaaacct tggcagcggt aatctcgcct caccaatatc tataccctga attcttaata
     9301 tttcttttgc aacagaatat aaatttccat gacaagaaag cattgcaaaa ataactttgt
     9361 tgatctcata ttgaatttct tttgcagtaa caaagtcacc tgagcgtatt aattcatctg
     9421 cttttatgaa caattcaggc atcactgcgt aagtaccgcc aatacctccg tctgcgccaa
     9481 ttgctctgcc agctacaaat tgctcatctg gaccattaaa gacaataaaa tcttctccac
     9541 ccgctgcttt gaaaatctga atatcttgtg taggcatgga ggaatttttg atacctatca
     9601 cttttttatt cttaatcatc ctcttaaata aatctaaagt caaggcataa cctgttgttc
     9661 ctggaatgtt gtaaataaca aagtctaaat ctgttgcatc aatcatgtca ttccaatatt
     9721 tttcaatagc atattcaggt aacctaaaat agatgggggg aattgctgct acagcatcta
     9781 ccccacattt ttctgcgtgt tttgcaagct caatactgtc ccgggtagat ggtgcagcaa
     9841 tatgagcaat gatgctaatt ttcccttttg caacttccat gacgttttca agaattattt
     9901 ttctttcttc tacattttga taaatacatt ctcctgaact tccacaaaca tacaagcctt
     9961 taaccccttt atcaatcaga tattgggtaa acagcctggt acgttcagga ctgacatccc
    10021 cattatcatc atagcaagca taaaatgccg ggaaaattcc tctatatttg tctagtctac
    10081 tcatgatatc cctcttttac tatttctttg ttcattaaat tggcaggaat ctttcctgaa
    10141 aagaaagctt cgattgtatc ggccataatt ttttgtgaac gtaaaccgga ttcagctgtt
    10201 tcagccccca tatgagacgt aacaaatacg ttgtccatag ataataaagg attgtccaaa
    10261 cttgggggtt ctaaacttaa tacatccagc cctgccccgc ggatcttttt ctcccgtaag
    10321 gcttcaatta aatctgcttc attgataaca gcacccctgc aagtattcac caaatacgct
    10381 gaaggtttca taagatttaa caatcttttg tcaatcattc ctcttgtttc atcatttaga
    10441 ggtacgtgaa gacttacaat atcactttgt aagaatatct cttctataga atgaacaacc
    10501 ttcacatcat attggtttgt atctgcttgc gcataagaat catagacaag gatttttaca
    10561 tcaaaaccgg aaagcattct ggctaattgt tttgcaatat tcccgaagcc aactaaacca
    10621 actacttttc catctagttc atttccagtt atagcacaag gccaaagacc tttttttgta
    10681 tcatctataa ctgtcttaaa acgtcttcct aaatttaata tatgcagcag ggcaatttcc
    10741 gctactgcgg cggaattagc tcctggtaca tttgcaacag gtattccagc ctcagtagca
    10801 gctttaagat taatattatc aatacctgca ccatatctct gaataaactt tagatgtccg
    10861 cgtacagccg gaagtgttct ttcattccat cgttcaaaaa cacttaccac tgcatcaaca
    10921 ctattcccca tttcaattgt ccctgcttca tcatcagcaa gaacgacttt ataaatcacc
    10981 tcaaagccac tgtcttttaa acgcttttcc aaatatgaca catcgacttt ttgaggatca
    11041 atatttaccc ataccttctt actcatgtcg gtttttctcc ttctggaaat aaaagcattc
    11101 attttacttt atttctgaac taatttgctt tatatctgct tccatttttt caactgcttc
    11161 atctactgtt tttgttcctg taacaacaga gttgagtaat tctgcaacag ggtaagtaaa
    11221 ataatgttta gcagcagtca ctctgccttc aatactcttt acaggatatc catagtaacc
    11281 agaagcgttt ttagcataat taactacttg ttgattgata ccttctgccc aaaattcatc
    11341 gttttctaca tcctggaata ttggtgcaaa tactggtgca gtaattttga agtattcatc
    11401 gtaccattca gcatctaaca tatatttaat gaaactttcc gcagattcta catctttaca
    11461 ggtgttcatt atagcaaagc cgctgatgaa ttccataacc actgagttat ctgttcctgc
    11521 aggaggtgct aaagctactg tattttcaag tatttcttta tatgcttcgt ctccttttaa
    11581 tgcattatat aacgtaggtg cattaaatac gattgctgat tcgcccatta aatatgtgct
    11641 gttatttcca cctggatccc aggttgaagc tgacggtggt atgatttctt ttccatacat
    11701 ttcagcatat tttttaatta attcttttgc tttttcactg ttaatggctg ctgtaccatc
    11761 ttcattaaag acataagccc cgttattcca cataatcata cggaatgtat tttcacagtc
    11821 ttcatctgta ggaccacaac caatacccaa accataaaaa ccatttttag gatctgatac
    11881 tgcttctgct acttcaaata cttcttccca ggttgtagga tattttgtaa tgcctttttc
    11941 ttctaattta tctttacgga taaacatcaa agttgaagaa ctataaaaag gaacaaagta
    12001 atttacacct tcaatttttg aaccttcacg aatagattca aaatatggtc tgtttgcatg
    12061 aatttcatct actaaacctg ttacatctaa gttaggaata ttaggataat agtttaaaac
    12121 tcttgtaatt gccccagaag ttaaagtaac atccggaaca tttgttcctg cttcaattgc
    12181 agcattcaat ttgggcacaa aatctgtagc cgctaaaagt tcaaatttaa catcaattcc
    12241 gctctcatct gcaaattgct gaactctctt ttgcagttct gcattagcat catcactaaa
    12301 aactttttcc agccaaaccg ttagttgctt accctgagtc tttgactcac cttgactagt
    12361 attgcttcca ccagtatttt cacccgtctg acctgcgtta tctttgcccc caccacaggc
    12421 tgaaagagaa accagtaaag caaccattaa aagaattgaa actagccttt tcttcataag
    12481 atccctccta aagttttaaa aatcttttat tttttataaa acttttatat aaatttcctg
    12541 caatatattt aagtctttat cgtatattgc cggaaaattt ataccacaga aattgtttaa
    12601 ccttttacgc ctccggccgt tgcaccgccc gttaaaaatc tcgatgaagc gatataaata
    12661 ataagtattg gaatacatcc tattatggaa cctgccatta tctgtcccca gggatataag
    12721 tcaccataaa tcaatcctgc tataatcaat gggaacgtct ttgcctgagt atctgttata
    12781 tttactaatg cgtacaaata ctcaccccaa cacatagcaa aagcaaaaat agctgtcgaa
    12841 ataactcctg gtatcgttaa aggaaaaata atctgataca tgattttaaa tttactgcaa
    12901 ccatctacca atgcagcctc ctcaatgtca tatggtattg aggatatgtg agggattaaa
    12961 atccaggcga cataaggaag tgtaaaagtc gggtatataa gcattaaacc cattatgtta
    13021 tctgataagc ccactctgga tacaaaaatg tacaaaggta tatataacac cgaggaaggc
    13081 agcaggtagc aaaacaatat aactttcgca aaggtatttc ttcctttaaa ttttaagcgc
    13141 gatattgaat acgctgtcgg ataagcaatg ataattgaca aaacggttac aacaagcgct
    13201 actaagaagc tgttttttaa tgcattcata aaaggtgttt caaaaattaa atacttaaag
    13261 ttatttaatg ttggattttt tggcaggaag gtaggtatct tgctataaac ttcaccctgt
    13321 gtttttaatg aagtattaag catccaatat ataggaaaca gagcaataat taagaataac
    13381 gctacagtta tccggtgtag aatggtcact aatttattct gcatatttct caccttcctt
    13441 acattttctc atccgcaagg gattttttcg ttatcaaact aataatcaat aagaatacgg
    13501 gcattgctaa cactgaaacc gctatacccc gtccaatctg catgcttgcc atagctgttt
    13561 tataactata gacattcatt acctgggttt gaccattagg tcctcctcca gtcaacaacc
    13621 aaaccgtttc aaattcgtta atggtccaaa tactggacat cagagttgtc aagaaaatgg
    13681 tatccatgat agagggaagg gtaatataaa agaatttttg ccataccttt gccccgtcaa
    13741 tatctgctgc ttcataataa tctttggaaa tcgtctgtaa ctttgccagg attgatatcc
    13801 caaagaaagg agttcctcgc catacattgg ctacaatgat tgtcgctaat gctaatcctg
    13861 ctgttccaaa ccagacaata tctttatcaa tgattccaac caattttaaa atgtgattta
    13921 taattcctcc tgttgaagca aataaccatt tccaatttaa tactgctaca acattcggaa
    13981 gagtccatgg tatcaataat aaagctcttg caatgtttct gcctttaaat tcaacattta
    14041 gaacgactgc cattaaaacc cctaaaagca acttaaacaa tagtgagatt gaagcaaatt
    14101 gaagcgtctt ccataaagat aataaatatt ccttatcatt aatcaattta atgtaattac
    14161 ctaatccaat aaagcttgcc tctcctccaa tcgccttatc ggtaaagctc aaagaaatcc
    14221 cccatatcat tggcataaat aatacacatg ccaaatataa aaacatcggg atattgagca
    14281 tgtaagggaa agatttcttt gaaccaggta attgcctctc catgttattt tgccggaatt
    14341 ttctaatgcg tgccatgtta tcctcccaca ttctttaata ttgattatta tctaataata
    14401 tccaattatt atacctattt tgtatatttt gtcgaccggt acgtaatatc ttgtaattac
    14461 attgtatatt ttgtcgaatt agatgtcaat agaaaaaaga aaaatttttt catttttatt
    14521 tttacaagaa aatttttttc actttgcaat aaaaaaacaa gcgattttaa atgataaacc
    14581 gcttgttttt aagcttacta tttccatgaa cttttcaaag aaactatccg gttaaataca
    14641 agcttttcat ccgttgtatc tttcgtatct atgcaaaaat aaccatgacg taaaaattgg
    14701 aattggtccc ctttcttagc ctcttttatg gaaggttcaa tgagacaatt ttttaatacc
    14761 tttaaagagt cagggttaat aactaattct cctgtctgtt catcttctat aaacagatgg
    14821 tcatataatc ttacttctgc cggaactcca tgttctgctg aaacccaatg aatggttcct
    14881 tttacttttc ttcccgaaaa accgcttcca cttttggttt ccggatcata agtacagtga
    14941 agttcgataa tttctcctgt tttttcatcc ttaatgactt tttcacattt aataaaatat
    15001 gcattcttta atcttacttc cttgccaggg gataaacgga agaatttctt tggtggatct
    15061 tccataaaat cttctctctc aatgtatatt actctggaaa aaggaacttg tcttgttccc
    15121 atttctggat tttcagcatt attttcaaca tccagcattt caacttgtcc ttccggataa
    15181 ttggtgataa ccaccttaag aggatctaaa acagccataa ttcttggttt tttaagtttt
    15241 aagtcttcac ggatacaatg ctctaaaaat gagatgtcta ctacaccttt atttttagca
    15301 acaccagttt tttgacaaaa atcttttatg gcttcaggcg tgtagcctct tcttcttaaa
    15361 cctgaaattg taggcatacg gggatcatcc caaccgtcta caattttttc ttcaactaaa
    15421 ctacggagtt ttctcttact ggttaccatg ttagaaactt caagccttgc aaactcgatt
    15481 tgtttaggac ggttttcaat ttcaagggct tccaaaaacc aattatataa aggcctatgg
    15541 tcttcaaatt ctaaagtaca aatggaatgt gtaatccctt caatagcatc tgataaagga
    15601 tgggcaaagt cgtacatggg ataaatacac catttatcac ctgtattatg atgttttaca
    15661 tgagcaatac ggtaaatgac agggtctctc atgttcatat taggagaagc catatcaatt
    15721 ttagcccgga gtaccctgga gccatcagga aattccccgt ttttcattct gataaataaa
    15781 tccagatttt cttctactga acggtttctg taaggacttt cttttcccgg ctcagttaaa
    15841 gtgcccctgt attccctgat ttcttcggca gttaaatcat ccacataagc cttacccttt
    15901 ttaatcagtt ctatcgcata ttcataaagc tggtcaaagt accaggaagc ataataaagt
    15961 ctgtcacccc agtcgtaacc cagccatctt acgtcttctt taatggattc tacatattct
    16021 acatcttctg ctgtcggatt ggtatcatca aatcttaaat tacaaagacc accatatttt
    16081 tcagccgtac taaaatcaag ccagattgcc tttgcatgtc caatatgtaa atagccattg
    16141 ggttccggag ggaaacgtgt atgaaccttt ttaaccacac cttctgctaa gtctttatca
    16201 atgatgtcat gaataaaatt agaatgatct tcatttattt gttttttatt agactccatt
    16261 aacagccgtc ctccttatag tatatcttcc tattcttagc tcatattata tatatcatac
    16321 tcaatcctaa cacaaattat aataggtttc aatctatctt aacataaaaa ataagttgca
    16381 cagaatatgt gcaacttatt ttatttcacc agatgtacta ataatcactt ctctgtaatt
    16441 aacaatcttt tcagcattaa gcatagcttt ttttacagct gcggcaatcc ctgtgcagca
    16501 aggcacttcc attctgacca cggtaatgct ttgtgggtca tgttttaata aaatttctgt
    16561 cagtttttca gtatagtaat tgacatcatc taacttagga cagccaatca tggtaatatg
    16621 attctttata aaatcctcat ggaaattacc ataagcatat gccgtacaat ctgctgcaat
    16681 cagccagtga ggatgatcca gataggtcgc atccggatta attaaactta actgtaccgg
    16741 ccactgcatt aactgagatt gaagttcaac tttagcttca tctttaactt catttttttc
    16801 tttaacaacg gctggctttc tttgaatggt ctttgccatg gttcctggac aaccacaggg
    16861 catttgtgct tttttaagct tttctctttc ggccattttt tgattcacca attcttcatt
    16921 ataagcctga ctttctcttt caataatttc tattgctcct accgggcatg caggcaaaca
    16981 atctcccaat ccgtcacaat attcatctga taccagtctt gccttgccat caatcagttc
    17041 aatggcgcct tcatgacatg catccacaca taaaccacag ccgttgcatt tatcttcatc
    17101 aatatgaaca atttttctta tcataactta ttcctccttt atttcttgat ttaattataa
    17161 acccttactc tgtttgattc tgtgatttaa attacattta atgaataaaa tattgactat
    17221 ttatcttaat gatatataat aaactcattg attattagaa attggggaga agttctatga
    17281 attcggattt ttcgaaagga tttaaagcag gtgttcctat tgctttagga tacttcccgg
    17341 tatcctttac ttttggttta attgcagtca atggaggaat tcctgtctgg cttgccatat
    17401 ttatttcgtt ttccaatctg acctcggcag gacagtttgc tggaacaaac ttaattattg
    17461 ctaatgcacc tattatagaa attgctttga caactttcgt tatcaacatc cgttatatgt
    17521 taatgtcttt atccttgtct caaaaaataa aatcaaatat gactttatta taccgctcta
    17581 ttattgcttt tggaataaca gatgagacct ttgcccttgc ttcccttgaa aaaaacgaac
    17641 tcaccttttc ttatatgatt ggcctgatat cagggccctt tgctggctgg gttttaggaa
    17701 ctgccgcagg ggctgtgact tgctcagtac tgccagaaca agttcaaagt gcgatgggta
    17761 ttgcccttta tgccatgttt attgctttag ttgttcctcc atcaaaaaaa tccaaagcac
    17821 ctctaatcgt tactataata ggaattttta ttagttcaat cttaaagtgg ttaccttata
    17881 tcaatcaaat ttccagtggc tttagaatta ttattgctac cattgttgct tctgcaattg
    17941 gagctttatt atttcctaaa ggagaagaag aattatgagt tacgctttta ctgcggtcat
    18001 tgttatggca attgtaacct atatcccaag ggttttaccc attgcaattt ttagaaaaaa
    18061 aataaaatca aaatttatat tatccttttt atcttatgtt ccttttgccg ttttagctgc
    18121 gatgactttt cctgatattc tttattctac aaatcatctt tcttcagccc tggcaggtat
    18181 gataacggca ctgattttat cttattttga aaaaggtctt atgactgttg ccctatgcag
    18241 catcctggtc gtttaccttt gggaaatctt tatataaaat tggccgttcc acaaatgacc
    18301 cattcggact gtttaataac aaaaagcaaa agagctggtt cctgaagaca aataactgtc
    18361 aggaaacagc tcttttattt atcaagtaaa attaatctaa acaatttatt aaatgaatac
    18421 gttctgcatc tacctgatct tttatgcttt gagaaggatt ccataaggaa tcatatctga
    18481 acagtataaa acctttatag ttactgcttt ttcttccttc ttctatcatc cttgaaagaa
    18541 tatcgctcgt accaatccat tcattttttc catttcctgc ccaggcatct tcctggccta
    18601 atttgtaagc tgcaagccct atgtaaagat caacatcact atttttgata agttgattcc
    18661 attcctggac aaccttatca tacggataat tagaatgatt aaaaccaaaa tatacctgag
    18721 ggcagatata atcaatataa ccagaattag aaagccattt ttcgacatca atatattgat
    18781 tattataatt acggcttata ataccctgag ggcttatacc aaattttata tcaggatcat
    18841 aggctttaat ggtcttataa acttctctta ctaattgatt gacattttcc cttctccatc
    18901 ccgcctgact gagatatcct ccttcctgca aatacttttg atagtctgct gcatcaaaac
    18961 tcatatccgt agtcggatag aaataatcat caaaatgaat accgtccacg tcataatttt
    19021 gaataatttc ttcaatacct ttaacaatta gattttggac atctttgctt gcaggattat
    19081 aataaatacc actgtttatt tttatcaccc ggttgctgcc gtcatttaac cactctaaag
    19141 ctttattatc agcacttaaa ggcttattat tattcctgat tcggtaagga ttaatccagg
    19201 cttcaatttt taagccctgt aaatgggctt cgtttaccat aatttcaaga gggtcgtaat
    19261 aaggatttac cccttctgtt cctgaaataa tataggacca ggggaaataa gaagaaggat
    19321 aaatggcatc tccaaaaggt ctgacctgaa caaaaacagt atttaaacct gcatctttta
    19381 tatttctgaa catctttctt atactttctt taaattgtgc ttctgtctta ttagggaata
    19441 tattttgcaa ttctaaataa gaaatccaaa cacctcttac ttctccttct atactatttt
    19501 caattttatt tccttcatta ttttcatcaa gataggcttc ctccattaat aactgtttta
    19561 actgactgtc tatcctggct gctgctttta atttattatt cgaaataacc cctttttcag
    19621 ccaaatgaat caataagctt ttattttcat ttttcatttt tgtaaaaaga caattatagg
    19681 acatcatcac aatataatct cttaaaacac ctgttttttc tttagctttt aataaagata
    19741 attcactgtc attaatcatg ccaatatgat gtgcaaaatc taaaacatta tttagatgaa
    19801 aatctccatc tctatcgcta taacccaggg ctcttaataa aaaagtcata tattgctctg
    19861 gagtaatctc gtcttttgca ccatataaat tgccgctaat accaaaagtc aaccctttat
    19921 tgtataaata agaaacggct ggacttgccc aggctggtac atccgcaaaa ggatgaggct
    19981 ttgacttata ttcttctgcg gcggtctttt ctgctcctaa taatctaacc agcatggctg
    20041 ctgcttctgc cctggtactt tttctgtcca gttcaaaacc tttgtcggtt cctaagaaaa
    20101 gacccagagc ttttaaactt tctgcatttt ttacataagt agcctgatta cttgcataag
    20161 ataacgaaat acttgaaaaa gataaggata ataataaagt gaagatcata aaatttctta
    20221 taaaaatttt gattttgccc attttttcct cctaaagcca taagatataa atacatatgt
    20281 tattataaca tattttgtca aaatccttct ttaagattat attaaaaagc tgtgacatct
    20341 gtatgaatat tattttaatt gtctaacaat tataagactg cagcaaaaaa gtgtgtctta
    20401 aagttaagtt aaactttaag acacactttc ttattgtaaa tttaaacttt tttcatagat
    20461 ttgataaata tcttctaagc taactttagc aggatgattt ttaagtttca aatcattgaa
    20521 ggtcatatct tgtgcataag ctataatctc gtcttttgta actttttctt tttctcctag
    20581 taaagaatct aaaaaatctc ctaattcttc aagattattt aaacctaaac agcttaatat
    20641 cctgtctacc ctttctctat ggggatgaat ttctaaaaaa gctttcatca aaagagcatt
    20701 ggcccttcca tgagcaatat tatgaaaata tgtcagggca taacccatac catggggtaa
    20761 agaagttcct gtttgtgcaa taaccattcc tgcgatggtt gaaacaagta agagtttttc
    20821 ccttaataaa taggtaaaat tctttttttt caaagcatcc atgcattccg aaaaaaggtt
    20881 gagtcctttt tctgcccaaa tatcactaaa cagattagct tttaccgtta aataaccttc
    20941 tactaaatgc gaaagggcat ccaatgcagt attaattgta atcgcttcag gcatagccat
    21001 catatattca ggatctaata aagctaagtc aaaaaatact ttgtgaacaa tattgatttt
    21061 tgttttcttt tgatgattgg tcaatatcgc atacggcgtt gtctcagaac cggtaccagc
    21121 tgtcgtagga acagcaatcc caggaagcgt tttcatgggc tttagaatat ataaatcttc
    21181 cttactgctg tcctcattgg ctattaaaag attgatggct tttgctgcat caagaggaga
    21241 acctccccct atagcaataa taaaatctat tttttccttt ttcccaaaat ctgcaccttt
    21301 ttctactgtt tctaaagaag gattttcttc tatttcatca aaaatactgt aagctattcc
    21361 attgcgcttt aatacttcta aaatatcatc taatgaacca tttttcttag ctgaatgttt
    21421 accagtcaca atcaaagctt ttttcccata ttctttaaat aaagatgcgt tctttttaat
    21481 aaccccttgc ccaaacagga tctttgtagg catcagatac tgaaattctt gcattgtcat
    21541 cttcccttct acacttatta ccgtttatta tggattatta tattgtatat tatcaaaaaa
    21601 gtccatttta ccaaaccatt at
//
LOCUS       DAOSUD010000035        21584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1594_length_21584_cov_27.386316, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000035 DAOSUD010000000
VERSION     DAOSUD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 21584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21584
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_21584_cov_27.386316"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      113..229
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            321..950
                     /locus_tag="PLG49_05990"
     CDS             321..950
                     /locus_tag="PLG49_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ16909.1"
                     /translation="MESIKKTIPHERKLFQTTNLVRMGLLSALAVVLMRFEFVVPGFP
                     PFLKMDLSDLPAVIGAIMIGPVAGVVIQLIKVLLYTVSGSETFGIGPLANFIVGAAYV
                     LPFGLIYKYKRNILGVVVGCIAGTISMAVIAAFMNYFVMIPFYSVAFGLSIDDIVAMC
                     AKVNSRVQDLKTLIVFSIVPFNILKAIIISMISIVLFVPLKPLLIKFNH"
     gene            1058..1498
                     /gene="tsaE"
                     /locus_tag="PLG49_05995"
     CDS             1058..1498
                     /gene="tsaE"
                     /locus_tag="PLG49_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOQ16910.1"
                     /translation="MIYETFSEEETKELAYKIACQAKKGDIYCLIGDLGAGKTAFAKG
                     LAKGLDIEEEIISSPTFTLVNEYQGRLPLYHFDVYRITDIDEMEEIGYEEYFYGDGVC
                     LIEWANLIEELIPREAMWIKIIKDLDKGENYRKIIIENKKEREN"
     gene            1495..2202
                     /gene="tsaB"
                     /locus_tag="PLG49_06000"
     CDS             1495..2202
                     /gene="tsaB"
                     /locus_tag="PLG49_06000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOQ16911.1"
                     /translation="MKILAIDSSGNVASVAVLEDEKLLGEMSTNYKKTHSQTLLPMIH
                     QLCQMIDLDLNSLDYIATASGPGSFTGLRIGASTAKGLAFALNKPVVAVPTLDGLAYN
                     IVYSDHLICPIMDARRNQVYTAYYLWEEDSLKRQSEYMAIDIENCLEKVKVYNRPVIF
                     LGDAVPVYKETIKEKMEDVKYFFAPPSCNMQRAASVGSLAFVYVREGKIQSAMEFVPF
                     YLRKSQAEREYEEKNKE"
     gene            2220..2660
                     /gene="rimI"
                     /locus_tag="PLG49_06005"
     CDS             2220..2660
                     /gene="rimI"
                     /locus_tag="PLG49_06005"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOQ16912.1"
                     /translation="MIQTVPMKEEDIGRVYEIEEKCFLTPWSKYSFKKELENPLTYYI
                     VAKKENDIIGYGGMWIIVDEGHITNIAVDPAFRNQGAGKAILTALLDEAERRKLAAVT
                     LEVRKSNHIALNLYKKFGFICAGIRKEYYHDTKEDAIIMWKYLS"
     gene            2793..5222
                     /locus_tag="PLG49_06010"
     CDS             2793..5222
                     /locus_tag="PLG49_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ16913.1"
                     /translation="MKKYRELSYKELKRYIDPSELDFKTTEDLEPLEGIIGQERAVKA
                     MEFGLNIKMRGYNIYMSGPTGSGKTSYAQSCIKDFAKNEPVPYDWCYVYNYSKPSNPI
                     ALRFPPGLGKEFRDDMNELIEYLLADIPKAFSSEEYEKEKSEIIKDYQNKRDDLLNQL
                     KEKAQKLGFAVKMTGAGIYFVPIVNGEVIEEEDYRNLGKEVQEQIDQLTDEIQSEVTE
                     TMKTIRNLERKARQKIEDLDYKIGLFAVGHHINYLKEKYKEYDRVIKYLEAVQEDVLE
                     NIQEFLEYEEDEEEAQPPVPAALMPFMVKSIEDVVKKYRVNLFVDNTEIKGAPVIVDF
                     NPTYNKLIGQIEYDNEFGNLTTDFMKIKPGLFHQANGGYLILQAKDVLNNSYSWEAIK
                     RVLKTKEVTIENLRELMGLTAVSSLKPEPIPVSVKVILIGSTYLYDLLYAYDEDFRKL
                     FKIRVDFDSEMDNNHENIKMLARFVHGFTKRENTAPFTADAIAKIAEYASRLAENQEK
                     MTTRFNSIVEILCEASIWSKMSNKKEITAEDIEKAITEKEKRSNLYEEKMEELIKDNV
                     IMIDCEGEKVGQINGLAILDTGDYRFAKPSRITATTYMGQSGIINIEKESEMSGNIHD
                     KGVQVLTGYLGQTYAQEFPLSLSCRICFEQSYSGVDGDSASSTELYAILSSLSGVPIK
                     QGIAVTGSINQNGEIQPIGGVSHKIEGFYKICKLKGLTGEQGVIIPYQNVKDLVLKDE
                     VIEAVKEGKFHIYPISHVDEGIEILTGVPAGQKDENNRYDENTIHGKVYAKLKAFYEK
                     TLEQNHSKENN"
     gene            5968..7044
                     /locus_tag="PLG49_06015"
     CDS             5968..7044
                     /locus_tag="PLG49_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol-1-phosphate synthase"
                     /protein_id="HOQ16914.1"
                     /translation="MGDKKIRLALVGVGNCASSLVEGIYFYKNAEIAKEVGGLMHYEI
                     GGYEPQDIQVVVGFDIDSKKVGKDVSEAIFAPPNCAYKIADVPYLNAPVLKGPVLDGA
                     PDHLRHYYGKDYFTVDDSQEPVDVAQALKDYKVDVILINLPTGSYNAARYYVECAKKA
                     GCGIVNGIPELLASDEKIAKGCEEAGVPILGDDWKSQIGATIVHRALAKLFEDRGIKI
                     KKSYQLNYAGNTDFINLVMRGETKHVTKHDAVASILKNDVPISPGFAFVDNQGDMKTA
                     SILIEGTKFGGAPVKLELRLEVEDAPNSAGISIDAVRCCKLALDRGLAGAIIEPSSYF
                     FKHPPVQFTDDICREKVDAFIKGE"
     gene            7209..8135
                     /locus_tag="PLG49_06020"
     CDS             7209..8135
                     /locus_tag="PLG49_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOQ16915.1"
                     /translation="MKNKVDIVVIGTIIKETIVFPDKQIGPVLGSPAAYSSLVMAAQG
                     VNVGIVTYYGSDLGKVFNELDVLDKRGILPYEYTTTNLLIYNEDGTKRVEYQKSTPQI
                     RFENIHKDYLEANYFKICPMNYEVDLDLVIKLHAMGKIIFIDLGGFGGATSDIRYSID
                     TSYGRKVITTLCNNSSIIKASEEDLKSIIPGKTAEEAAEILIDMGAPKVVVTLGSKGA
                     LYKIGKDKPQYLKPFVAVSENPDGSLDFTGAGDSFGAGFMASYMQDQDIRKAILNGNA
                     TASLVIQRSGGCTYDRMPSKERVKERLQQQNI"
     gene            8201..9376
                     /locus_tag="PLG49_06025"
     CDS             8201..9376
                     /locus_tag="PLG49_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ16916.1"
                     /translation="MARQIGVGIIGAGSIADIGHCPSIAALENAKLAALCDPNKEILD
                     KMTKKWEPEKSYTDYQAMLEDKNVEVVIVGSPNRYHAQQAIDAMRAKKHVIVEKPFAC
                     THEEAWKMVDVSKEEGVFLMAGTNQRFWEQNVIARKLIDEGFIGKPQMGRSSLHEGWN
                     LYHEKLSFTRFRQYPNEAGAGALFDLGAHRADLLMFLMGSKPKRVIGMIKAFGTDYTP
                     LDDSFWIMIEFENGATGVISGDRFSPAVSNISEVYGTEGTIFTASEATNPFQTAPLAV
                     FTSKNFKQSELPEIVREYRYPQLFWSEDIMQPDGYVPKRWVPIYPKRGWAYKSMLEHF
                     LNCVEKDVPPSLTPEESALVTDILVGAYKSMETKSWVEVGRDMENYIVPLYDAPEIV"
     gene            9422..10522
                     /locus_tag="PLG49_06030"
     CDS             9422..10522
                     /locus_tag="PLG49_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOQ16917.1"
                     /translation="MKKLLAILVTMAMLVSLAACGGKDSKKTGDTDGSTNAGTETPAK
                     QEGKTDKKIKIGYSFPTINNEFWVTSLEHVQAASAALGFELIYDDCNNDQAEQLNDVE
                     SMISAGIDGLVLGPQDASVVAGILDACKKNDIKVVIIDRWPGDNLVAGEDYIAFIGPN
                     DEEAGYQIAMSLINAGVKKMIGIGGYQNTSVAEGRRAGLMKALEEHPEVELLQYEWAG
                     ENWDDGDEYFRNMYQAHPDLEGVWCYNDSLALASVNVCKELGIIDKVKVGGMDLLSPA
                     VESMQAGELWFSTGGHYMQAAFAAVIAFDEINGNKYTGDPVVSLNLLNVSQENLDKFV
                     EKYIKNEQPIDWKNLSKTFNPDAKLIFELTLD"
     gene            10673..12178
                     /locus_tag="PLG49_06035"
     CDS             10673..12178
                     /locus_tag="PLG49_06035"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOQ16918.1"
                     /translation="MGAAVVFKGIKKYFEGTKALDWDPEDVMEVFPGEIHGLVGENGA
                     GKSTLFQVLMGIYKRTAGEIYLFGQPVEINNVHDAERAGISIIMQQPNFAYNMTVAEN
                     IFMGRDKEFTNKAGLINWKKQNEAAAKILAEYNFSHIKPTDILSDLGFEERKQVEIVR
                     ALSVKPKILLVDETSAAVSKKSVEILYKLLREQRDKGTAVIYISHFIDEVYELCDRVS
                     VLRDGKLVAKMVVKDTTPEMIINNMVGRDISGESYRSDNNDSIGDVMLEVKNFTKEGV
                     FEDINLTIRSGEIVGIAGIGGCGSDEFGRVLFGYEQPTSGEMIYKGKNGHMKSPLEAI
                     NNGIAYIPKDRDLEGLFLIYDLVMNISAANLKKMHKRGIINNKLEVQVAKDSIKRLKI
                     KTPSERTAVSNLSGGNRQKVSIAKWVANESSLLIVNSPTRGVDVGAKYEIYKILESLK
                     NEGKAILLISDELPELLGMCDRIYSFRKGRNSGEFTRGVNFTEEAVVATMV"
     gene            12208..13173
                     /locus_tag="PLG49_06040"
     CDS             12208..13173
                     /locus_tag="PLG49_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ16919.1"
                     /translation="MGMTNTKNKAELIKDKLKKNISLTAFIILFIFFSLTTDGKFLTS
                     NNIQTLLGASIDLLICALGATFIIMLGSIDLSSGSMISLCGVIAAKIYETTTSTLAAV
                     LAAIAVSVLFYGIMGVAHTRLQVPTFIVTLGFLSIARAVVTMISSGTITMIPFDSPFK
                     KYFGLKPWIIILGLVMFIIVWLIERYTLFGRYTRLIGGDERVAKLSGINVNKQKVLVF
                     LFAGLLTAIGGVVMAARIGSGSPAIGQGYELNVISAVVLGGTSQRGGIGGVRGTLIGA
                     LTLAVLSNGLVLLGMPSEVQLFIKGIVLILAVYVTLERNPNAITK"
     gene            13205..13834
                     /locus_tag="PLG49_06045"
     CDS             13205..13834
                     /locus_tag="PLG49_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOQ16920.1"
                     /translation="MNLNTKIDIIKNCKEQILKEAEALNRVAKQVDEAYIEACEAILK
                     CEGRVIVTGLGKTGHIGKKLAATLASLGIPSFFVHSCESLHGDMGMITKDDVVIMISN
                     SGKSSEILSMIPALKIIGPTTISITRDKNSPLARATDIKILCDAGEEIDHLGLAPTAS
                     STAALAIGDAIATVVSWIKGFQREDFALSHPGGALGQQLIKEYTERISQ"
     gene            13935..15107
                     /locus_tag="PLG49_06050"
     CDS             13935..15107
                     /locus_tag="PLG49_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ16921.1"
                     /translation="MNKKLLYTYCILMMLFVISLCNTTQGIILSDYIVYYALESSKQG
                     LVSAFQSTGNIIGLIIIILLSGKYKKSTMLAISAALIPVIFFIIGNKPALFILLLSYA
                     FFGIVFALWDSLGSSLMADIHTINSSRYMNLLHGFFALGGIIGPLIFQSLGTEGFSWD
                     QMLRLAGIISLVAVFLFTLGLVSAKKLIIENKDNSSDILITDIKTFFREKRNRIILIS
                     AFLYGAHQIGITVWMTRYISEYLGTPQWGPAALSLFWVGIAISRLVASRLSLEPAKWI
                     FFGHLISGIVIAIGVLSENGFIMFICCGLSGLAEGAILPMTLDMACNWRKGKSSLGSS
                     MILFVHYVGFIVTPPLIGALIFTYGIKVGMIMPAILSIVATLFTYFLTRKGRERNV"
     gene            15100..16638
                     /locus_tag="PLG49_06055"
     CDS             15100..16638
                     /locus_tag="PLG49_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOQ16922.1"
                     /translation="MYDLTENYLKLQNGSDIRGIALEGVPGENVNLTPEIARNITSAF
                     ITYLSKKENKSAESLEIAVGHDSRISASLLKAGVLEGIQNMGSTAYDCSLASTPSMFM
                     STVLEGLEFDGGIMITASHLPYNRNGMKFFTKDGCLESKEIREVLEIAIEIAKKEPGK
                     AEHIKEVQKLDLLSYYSDYLKKVIKKEVGSEDEDKPLKGLHIVVDASNGSGGFFVKKV
                     LEPLGADTTGSACLEPDGTFPVHEPNPENRQAMEYIKKITLEHHADLGIIFDTDVDRM
                     GAVFSNGKEINKNAIVALMSAIVASQHPNTTIVTDSVTSEQLTDFLENKLNLKHHRFK
                     RGYKNVINEAIRLNQEGIETHLAIETSGHGALKENYFLDDGAYMAVKIICELVKCKRQ
                     GKALEDLLEGLKEPEDSIEIRIPIKVDNFKEYGQEVLEEFKKFAGENDKFNLVKNNYE
                     GVRITFNDDEVSGWMLLRMSLHDPILPMNIETDNKGGVAIALKRLAPFFEKFDGLDIS
                     VLSK"
     gene            16753..17691
                     /gene="fba"
                     /locus_tag="PLG49_06060"
     CDS             16753..17691
                     /gene="fba"
                     /locus_tag="PLG49_06060"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOQ16923.1"
                     /translation="MALVTTKEMFKKAYKGGYAIGAFNVNNMEIIQGITEAAQEENSP
                     VILQVSAGARRYAKHNYLMKLVEAAVMEVDIPIALHLDHGEDFEICKSCIDGGFTSVM
                     IDGSRHSFEENIKLTRKVVEYAHAHGVVVEGELGVLAGIEDDVNVSVENSSYTNPDEV
                     EEFVERTGVDSLAIAIGTSHGAYKFKPGQKVELRFDILEEIQKRLPDFPIVLHGASSV
                     NQEYVEMIKQYGGQMEDAMGVPEEMLRKAAKSAVCKINIDSDIRLAMTAAIRKYIMEH
                     PEDFDPRQYLKPAREAVKELVKHKIRNVLGCSNACK"
     gene            17710..18378
                     /locus_tag="PLG49_06065"
     CDS             17710..18378
                     /locus_tag="PLG49_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOQ16924.1"
                     /translation="MKKKAVIFDMDGLMIDSERVTYDGYVKICREFGYEMTLDFYKTL
                     LGLRINVISQKLKEHFGKFFPSEEIIRRVHIYIEEYFQEKGVPLKTGLQEILQYLKSR
                     DYKLIVATSTARIRAEKILKNAGISQYFDGYVCGDEVVNGKPDKEIFIKACEKAKVKP
                     EEVYVLEDSEMGILAASRANISCICVPDMKYPSEEMEAKTYKVANNLLEALEIIKEDE
                     KRFE"
     gene            18393..18782
                     /locus_tag="PLG49_06070"
     CDS             18393..18782
                     /locus_tag="PLG49_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ16925.1"
                     /translation="MLKQFHHLCLVSSDYEATIKFYVDTLGFTLYRESFSNNWNGRKL
                     ELYYNGQYMLEIFVVEKAKRRGQNPGEVYAGLNHFSFLVDDVEAFVRKLHDDNIETSE
                     VKLDKNTGKKYAFFYDPDGTKLEIYES"
     gene            18878..19537
                     /gene="rpe"
                     /locus_tag="PLG49_06075"
     CDS             18878..19537
                     /gene="rpe"
                     /locus_tag="PLG49_06075"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ16926.1"
                     /translation="MIKLAPSILSADFANLGRDVRALHDAGVDYIHIDIMDGGFVPVI
                     TFGSQLVKAIRKYSDKVFDVHLMVNNPDAHIKNFVEAGADIISVHAESCVHLHRTIQC
                     IKNYGLKAGVVLNPSTPLSSIEYILEDVDMVLIMSVNPGYGGQKFIDSAIRKISALRE
                     MINSRGLNTDIEVDGGITFENVQEVIRAGANVIVAGSSVFKGDIHKNIKEFNRLFKEV
                     N"
     gene            19537..20376
                     /locus_tag="PLG49_06080"
     CDS             19537..20376
                     /locus_tag="PLG49_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ16927.1"
                     /translation="MDKLELQKKANKIRQNIIRSIHSAKAGHPGGSLSSADILTYLYF
                     VELKNIDPKDPKKSDRDRFVLSKGHAAPAYYATLAEKGYFDEKDLTSLRQIGSHLQGH
                     PDMKGTPGVDMSSGSLGQGVSAAVGMAISAKISNENYRVYALLGDGEIQEGQVWEAAM
                     LAGHRKLDNLVVIVDNNNLQIDGTVEEVCSPYPIDKKFEAFNFHVIHADAHDFDSLEA
                     AFNEAKQVKGKPTAIIAKSVKGKGVSFMENKVSWHGVAPNDEEFEKAMEELKKAGEAL
                     CQK"
     gene            20364..21305
                     /locus_tag="PLG49_06085"
     CDS             20364..21305
                     /locus_tag="PLG49_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOQ16928.1"
                     /translation="MSEVKKIATRESYASALVELGKKYDNVVVLDADLAGATKTVKFQ
                     KEFPERFIDCGIAESNMMGVAAGIASTGKVPFASTFAMFAAGRAFEQVRNSIGYTKLN
                     VKIGATHAGISVGEDGASHQCNEDIALMRTIPGMVIINPSDDIEAKAAVFAAYEHYGP
                     VYMRFGRLAVPVINDNPDYKFELGKGITLREGNDLTIIATGLCVSAALEAADQLKAEG
                     INARVINIHTIKPIDEDIIIKAAKETGKIVVVEEHSIIGGLGSAVSEVLSENYPTLVK
                     RIGIRDVFGESGPAVQLLKKYGLDAEGIYKQVKEFLQ"
BASE COUNT         7509 a         2977 c         4516 g         6582 t
ORIGIN      
        1 catggaagaa acctatattc aagaaactta ccggtatgaa attttataaa tattttaata
       61 ataaatttga catcaataaa tacatatagt ataatgaata aaatttaata tgaagattct
      121 tcagggcagg gtgaaattcc cgaccggcgg tgacagtccg cgagtgcgaa agcactgatt
      181 tggtgaaatt ccaaaaccga cagtatagtc tggatgggag aagaattgtt ttttgttttt
      241 gaaaaacagt cagtctgaag aatcttcagg ctgtttttta tttgtccgat aaaagagaat
      301 tttaaaagga gagattcaat atggaaagta tcaaaaaaac tattcctcat gaaagaaaac
      361 tgtttcagac gaccaattta gtaagaatgg gacttttatc agcccttgca gttgttctta
      421 tgcgttttga atttgttgtt cctgggtttc cgccattttt aaaaatggat cttagtgatt
      481 tgccagcagt aattggagcg atcatgattg ggcctgtagc cggagtggtt attcaactga
      541 ttaaagtttt gttatatact gtttcaggaa gtgaaacctt tggtataggt cccttagcta
      601 attttattgt aggtgctgca tatgttcttc cttttggtct tatttataaa tataaaagaa
      661 atatactagg ggtagttgtt ggctgtattg cgggaactat aagtatggct gttatcgcag
      721 catttatgaa ttactttgtt atgattcctt tttattcggt agcctttggt ttatcaattg
      781 atgacattgt tgcgatgtgt gcaaaggtta actccagggt tcaggattta aaaactttga
      841 ttgtgttttc aattgttcct tttaatattt taaaagcaat aattatttct atgattagca
      901 tcgttctttt cgtaccatta aaaccccttt taataaagtt taatcattaa aaagtaaacg
      961 atgaattatt atggaacggt cttgaccgtt cctttaatta tttatatatt tattaatagt
     1021 gtatcattat gatatgattg aaaggaatta aagatatatg atatatgaaa ctttttcaga
     1081 agaagaaact aaagaacttg cttataaaat agcctgtcag gcgaaaaaag gtgatatata
     1141 ttgcctcatt ggggatttgg gagcaggcaa gactgctttt gcaaaaggct tggccaaagg
     1201 attggacata gaagaagaaa tcattagcag tcctactttt actctggtta acgaatatca
     1261 gggaagactt ccattatatc actttgatgt ttatagaatc acagatattg atgaaatgga
     1321 agaaataggt tatgaggaat atttttatgg tgacggggtt tgtttgatag aatgggcaaa
     1381 cctgatagaa gagttaattc cccgggaggc tatgtggatt aaaatcataa aggatttaga
     1441 taaaggggaa aattatcgta aaataattat tgaaaacaaa aaggagcggg aaaattgaaa
     1501 atattagcta tagattcatc gggtaatgtt gcttcggttg ccgtattaga agacgagaag
     1561 cttctaggcg aaatgagtac aaattataaa aaaacgcatt ctcaaacctt gcttccgatg
     1621 attcatcagt tgtgtcaaat gattgatctg gatttaaatt ctttggatta tattgccaca
     1681 gccagtggtc cggggtcttt taccggtctc agaattggag catctacggc aaagggatta
     1741 gcctttgctc ttaataagcc tgttgttgca gttccaacgc ttgatggatt ggcatacaat
     1801 attgtatata gtgatcattt aatctgccct attatggatg cgagaagaaa tcaagtttat
     1861 actgcttatt atttatggga agaagattct ttgaaaagac aatcagaata tatggcgatc
     1921 gatatagaaa attgcttaga aaaggtcaag gtctataaca gaccagtgat ttttttaggg
     1981 gatgcagtac ctgtctataa agaaaccatt aaagaaaaaa tggaagatgt gaaatacttt
     2041 tttgctcctc catcttgtaa tatgcaaagg gcagcttctg ttggcagttt agcttttgtt
     2101 tacgtaagag aaggaaaaat tcaaagtgcc atggagtttg ttccctttta tttgaggaaa
     2161 tctcaggcag agagagaata tgaagaaaaa aataaagaat aacttaagtg gggattttta
     2221 tgattcagac agttcctatg aaagaggaag acataggcag agtttatgaa atagaagaaa
     2281 aatgcttttt gactccctgg tcaaaatatt cttttaaaaa agaactggaa aaccccttaa
     2341 cctattatat cgttgccaaa aaagaaaatg atataatagg ctatggaggc atgtggatta
     2401 ttgttgatga aggacatatt accaatattg cagtagatcc tgcattcagg aatcaggggg
     2461 caggtaaagc catattaact gccttgcttg atgaagcaga aagaagaaaa ttggcagctg
     2521 ttactttaga agtcagaaaa agcaatcata tagccttaaa tctttataaa aaatttggct
     2581 ttatatgtgc aggaataaga aaagaatact atcatgatac aaaagaagat gccattatta
     2641 tgtggaaata tttatcataa tattaaaaaa gtcttcaaat caatgaagac tttttttgta
     2701 gattttttca aaaaaaaaaa taaaatcttt agaaataaga ggaaatagat agttggtatt
     2761 gaatataata atatatagaa aggagtggaa atatgaagaa atatagggaa ttaagctata
     2821 aagaattaaa gagatatata gatccttctg aacttgattt taaaacgaca gaagacttag
     2881 aacccttaga aggaattatt ggtcaggaaa gagccgttaa ggccatggaa tttggattaa
     2941 acattaagat gagaggctac aacatttata tgtcaggccc aaccggctcc gggaagacca
     3001 gttatgcaca aagttgtata aaagattttg ccaaaaatga accagttcct tatgactggt
     3061 gttatgtata taattacagc aaaccaagta atcccattgc cttacgtttt ccaccgggct
     3121 tagggaaaga attcagagac gatatgaatg aactgataga atatctcctt gcggatatac
     3181 ccaaagcctt tagttcggaa gaatatgaaa aagaaaagtc agaaatcata aaggactatc
     3241 agaataaaag agatgatctt ttgaatcaat taaaagaaaa ggcgcaaaag ctaggctttg
     3301 cggttaaaat gactggtgcg gggatatatt ttgtgccaat tgttaatgga gaagttattg
     3361 aagaagaaga ttatagaaat ctgggaaaag aagttcagga gcaaatcgat caattaacgg
     3421 atgaaataca aagtgaagtt actgaaacca tgaaaaccat aagaaatctg gaaagaaagg
     3481 caaggcaaaa aatcgaggat ttagattata aaataggatt atttgccgta ggccatcata
     3541 taaattactt aaaggaaaag tataaggaat atgacagggt cataaaatat ttggaagcag
     3601 ttcaggaaga cgttttggaa aatatacagg aatttcttga atatgaagag gacgaagaag
     3661 aagcacagcc acctgtacca gcagctttga tgccatttat ggttaaaagt attgaagatg
     3721 tcgtaaaaaa atacagggta aatctttttg ttgataatac agagataaaa ggagcacctg
     3781 ttattgttga ttttaatcca acctataata aattaattgg acaaatagaa tatgacaatg
     3841 aatttggcaa tctgacgact gattttatga aaattaagcc tggcttattt catcaggcca
     3901 atgggggcta tctcatttta caggcaaaag atgttttaaa taattcttat tcatgggaag
     3961 ccatcaagag ggtgttaaag actaaagaag taaccataga aaatttaagg gaattaatgg
     4021 ggcttacggc tgtttcttct ctgaaaccag aacccattcc agtatctgta aaggttattt
     4081 taattggaag cacttatctg tatgatttat tatatgctta tgatgaagat tttagaaagt
     4141 tatttaagat tcgtgttgat tttgattccg aaatggataa caatcatgaa aacattaaga
     4201 tgctggctcg ttttgttcat ggctttacta aaagggaaaa tacagcgcct tttacggcag
     4261 atgctatagc aaaaattgct gaatatgctt ccagattggc agagaatcaa gaaaagatga
     4321 ccactagatt taatagcatt gttgaaatat tgtgcgaggc aagcatttgg tctaagatga
     4381 gtaataaaaa agaaattact gcagaggata tagaaaaggc cattactgaa aaagaaaaac
     4441 gctctaattt atatgaagaa aaaatggaag aattaataaa agataatgtt ataatgattg
     4501 actgtgaagg tgaaaaagta ggtcagatca atggtctggc gatactggat acaggagatt
     4561 accgttttgc aaaaccttca agaattactg caaccactta tatgggacag tcgggaatta
     4621 taaacattga aaaagaatct gaaatgagcg gaaatattca tgataaaggt gttcaagttt
     4681 taacgggtta tttaggtcaa acctatgcac aagaatttcc cctttctctt tcctgcagga
     4741 tttgttttga acaaagctac agtggtgtag acggggatag tgcatccagt actgagttat
     4801 atgcaatttt atcgagcctt agtggagtac ctataaagca aggtattgca gtaacaggct
     4861 ctattaatca aaatggagaa atacagccta ttggtggcgt ttctcataaa atagaaggtt
     4921 tttataaaat atgcaaatta aaaggcttaa caggagagca aggcgttatt attccatatc
     4981 aaaatgtcaa ggatttagtc ttaaaagatg aagtcattga agctgtaaag gaaggtaaat
     5041 tccatatata tcccatatcc catgtagatg aaggtattga aatactaaca ggagtccctg
     5101 ccggacaaaa ggatgaaaat aaccgttatg atgaaaacac tattcatggc aaggtttatg
     5161 ctaaattaaa agctttttat gaaaaaactt tagagcaaaa ccacagcaaa gaaaataatt
     5221 aattatggaa aaaggagtgc tgacctttag gtaagcattc cttttttgta aggtattcta
     5281 taatctgtag taattctata taatgcaaat taaaaataag atgattgaag tttataaatc
     5341 aacgaaagta ggcttaacag ttcttgtttt ggaataaatg tacaaaaaat aaaccaatct
     5401 tttttgcaat tttaacaata gacattatgt aatagatgag ttaaaatcta attacaaaac
     5461 aaaaagatta tgttattaaa ctaatgatgt tcaaaatata catacaataa ataaatgaat
     5521 cagtattttt gaacaataat caagataagt aatagattac aagtagaaca aaatgaagtt
     5581 aaaacgattc actttttttt aagagaatac tttaacgtta actttaatgc ttaatttcca
     5641 aaaaccgagc tttgatattg taaaataata catcttttag aacatgaaac attgaactgg
     5701 aaataatatg aaataacaac agcactttaa cgtttactgt gatgattaat ttacaaataa
     5761 taatctatat attattttgg attaatagta aattattatg tgattgtgtt tataaccttg
     5821 ttccttgttt catattattt tacaaaaaga ctttaacgtt aactttaata tttatcatac
     5881 aattataagg gttttattta tatcttatta ggcaaaaagt tgaggagcta caacaaattt
     5941 tatttataaa tcaaggaggc aaaaatcatg ggagacaaaa aaatcaggct ggcactggtt
     6001 ggagttggta actgtgcgag ttcgctggta gaaggtattt acttctacaa aaatgctgaa
     6061 attgcaaaag aagtcggagg tttaatgcac tatgaaattg gtggatatga acctcaggat
     6121 attcaagttg tagtgggctt tgacattgat tctaaaaagg ttgggaaaga cgttagcgaa
     6181 gcaatttttg ccccaccaaa ctgtgcttat aaaattgctg atgttcctta tctgaatgct
     6241 ccagtattga aaggacctgt tcttgacgga gcgccagatc atctgcgcca ttattatggc
     6301 aaagattact ttacggttga tgacagtcag gaacctgttg acgtagcgca ggcattaaaa
     6361 gattataagg tagatgtaat ccttattaat ctgccaactg gttcatataa tgctgcaaga
     6421 tattacgtag aatgcgctaa aaaagctggc tgtgggattg taaatggtat tcctgaatta
     6481 ttagcttctg atgaaaaaat cgcaaaaggt tgtgaagaag ctggtgtacc tattttaggt
     6541 gatgactgga agagccagat tggtgctacg attgttcaca gggcattagc aaaattgttt
     6601 gaagacagag gaataaaaat taaaaaatct tatcagttga attacgcagg aaatacagac
     6661 tttattaatt tagtaatgcg cggtgaaaca aaacatgtaa caaaacatga tgcagttgct
     6721 tctattctta agaatgacgt tccgatttca ccaggatttg catttgttga taatcagggg
     6781 gatatgaaga cggcttccat tctgattgaa ggaacaaagt ttggtggagc gccagtaaaa
     6841 ttagaattgc gcctagaagt agaagatgca cccaactcag caggtattag cattgatgct
     6901 gttcgttgct gtaaacttgc tttagacagg ggacttgcag gtgcaatcat agaaccatca
     6961 tcctatttct ttaagcatcc tcctgtacaa tttacagatg acatatgcag agaaaaagta
     7021 gatgcattta ttaaaggaga ataagattaa aatttacatt gggctttatt ctgaaaagtg
     7081 agaggatctg ttttcacatg atcactcctt tcctgactct ttcacctccc tcattgaaat
     7141 aggtgaatga tttcaaaaag atccttctca tttttcagct tataaaaaat atattgggtg
     7201 ggtcaattat gaaaaataaa gtagatattg tggtaatagg aacaattata aaagaaacca
     7261 ttgtatttcc agacaaacaa attggtcctg ttcttgggag tccggcggcc tattcaagtt
     7321 tagtaatggc tgcacaaggt gttaatgttg gaattgtgac ctattatgga agtgatctgg
     7381 gaaaagtatt taatgaatta gatgttctgg ataaaagagg tattctgcct tatgaataca
     7441 cgacaacaaa tttattaatt tacaacgagg atggaaccaa gagagtagaa taccaaaaaa
     7501 gtacgcctca aataaggttt gaaaatattc ataaagatta tttagaagca aattacttta
     7561 aaatttgtcc tatgaattat gaagtagatt tagacttagt tattaaactt catgcaatgg
     7621 gtaaaatcat atttatagat ttgggaggct ttgggggagc taccagtgac ataaggtatt
     7681 caatagacac ttcttatggc agaaaggtca ttacaacctt atgtaataac agttcgatta
     7741 ttaaagccag tgaagaggat ctgaaatcaa tcattcctgg caaaacggca gaagaagcag
     7801 ctgaaatttt aattgacatg ggcgctccta aagtagtcgt tacccttgga agtaaaggtg
     7861 ctttgtataa aataggtaag gacaaacctc agtatttaaa accttttgtt gctgtcagtg
     7921 aaaatccgga tggttcttta gattttactg gagcaggtga ttcttttgga gcaggtttta
     7981 tggcctctta tatgcaggat caagatatta gaaaagcaat cttaaatgga aacgctacag
     8041 catctttagt tattcaacgt tcaggcggct gtacttatga cagaatgcca tctaaagagc
     8101 gggtaaaaga gagattgcag caacaaaata tttaattaac agtgtataag aggtaaaaat
     8161 aataacttat taaattatta aaaggggtgg tgcattgaat atggcaagac aaattggtgt
     8221 aggaattatt ggtgcggggt caatagctga cataggacat tgtccttcta ttgctgcatt
     8281 agaaaatgca aaattagcag ctctttgtga tccaaacaaa gaaatcctgg ataaaatgac
     8341 aaagaaatgg gaacctgaaa agtcatatac agactatcaa gctatgctgg aagacaaaaa
     8401 tgttgaagtt gttattgtgg gttctccaaa cagataccat gctcaacaag ctatcgacgc
     8461 aatgagagct aaaaaacatg ttatcgtaga aaagcctttt gcatgtaccc acgaagaagc
     8521 atggaaaatg gtagatgtaa gtaaagaaga aggcgtcttc ttaatggcag gaacaaacca
     8581 acgtttctgg gaacaaaatg ttattgcacg caaactgatt gatgaaggtt ttattggaaa
     8641 acctcagatg ggtcgttctt cacttcacga aggttggaat ctctaccatg aaaagttatc
     8701 ctttacaaga ttccgtcaat atcccaatga ggccggagca ggtgcattat ttgatttagg
     8761 tgcacacaga gcagatttat taatgttcct tatgggaagt aagcctaaac gtgttattgg
     8821 tatgattaaa gcatttggta cagattatac gcctctggac gactctttct ggattatgat
     8881 tgagtttgaa aatggtgcaa ctggagttat tagtggcgac agattctcac ctgctgtatc
     8941 caatatttct gaagtttatg gtacagaagg aacaattttc acagcttctg aagcaaccaa
     9001 tcctttccag acagctccat tagccgtatt tactagtaag aatttcaagc agtctgaact
     9061 acctgagatc gtaagagaat accgttatcc tcaattattc tggtcagaag atattatgca
     9121 acctgacgga tatgttccaa aacgctgggt acctatttat ccaaaacgtg gctgggcata
     9181 caaatcaatg ttagaacatt tcctgaactg tgtagaaaaa gatgtaccac catctcttac
     9241 tcctgaagaa agtgcactgg ttacagatat actggtaggt gcatataagt ctatggaaac
     9301 caagtcatgg gttgaagttg gacgcgatat ggaaaattat attgttccac tttatgatgc
     9361 tccagaaatc gtgtaatatt taaaaattat taatataaaa aaatcaaatg ggggtaagtt
     9421 tatgaaaaaa ttattggcaa ttctcgtaac tatggcaatg ttggtgagtt tagcggcttg
     9481 cggaggaaaa gacagcaaga aaacaggaga tactgatggc agtacaaatg caggaacaga
     9541 aacaccagct aaacaagaag gaaaaacaga taaaaagatt aaaataggat attcattccc
     9601 taccatcaac aacgagttct gggtaacttc cctggaacat gtgcaggcag catctgcagc
     9661 tttaggattt gagctcattt atgatgactg taacaatgac caggcagaac agttaaatga
     9721 cgtagaaagt atgatatcag ctggtattga cggacttgtt cttggaccac aagatgcatc
     9781 tgttgtagct ggaatattgg atgcatgtaa gaaaaatgac ataaaagttg ttattattga
     9841 ccgttggcca ggcgataatt tagtagctgg agaagattac atcgctttta tcggaccaaa
     9901 cgatgaagaa gcaggttatc aaattgctat gtcattaatc aatgcaggtg ttaaaaagat
     9961 gattggtatt ggtggatatc aaaacacctc cgttgctgaa ggtcgtagag caggtttaat
    10021 gaaagcctta gaagaacatc cggaagttga attattacaa tatgaatggg ctggggaaaa
    10081 ctgggacgac ggagacgaat acttccgtaa tatgtaccag gctcatcctg acttagaagg
    10141 tgtatggtgt tataacgact ctttagcttt agcatctgtt aatgtatgta aagaattagg
    10201 catcattgat aaagttaaag ttggtggtat ggacttatta tcaccagcgg ttgaatccat
    10261 gcaggcagga gaactctggt tctcaaccgg aggacactac atgcaggcag cttttgcagc
    10321 agttattgct tttgatgaaa ttaatggaaa taaatatact ggtgatccag tggttagctt
    10381 aaacttatta aacgtttctc aagaaaactt agataaattc gtagaaaaat atattaaaaa
    10441 cgaacaacct attgattgga aaaatttaag taagactttc aatccagatg caaaattaat
    10501 ttttgaatta actcttgact aagaatttta aggattatta aagcaatttg agtggtcaat
    10561 aataatatta attgttaatt gttgttgtga aatcccttgt cgggtaacct tagcttttgt
    10621 tacctgacaa gggagattta acaaaaagat ataaatacgg ggaggaatca ttttgggtgc
    10681 tgcagtggtc tttaaaggaa ttaagaaata cttcgaaggt acgaaagcat tagattggga
    10741 ccctgaagac gtcatggaag tattcccggg agaaatacat ggcttagtag gagaaaatgg
    10801 agcaggaaaa tcaactttat tccaggtttt aatgggaata tacaagcgta ctgccggaga
    10861 gatttattta tttgggcagc cagttgagat aaacaatgtt catgatgctg agcgggctgg
    10921 gatatccatc ataatgcaac aacctaattt tgcctataat atgactgtag cagaaaacat
    10981 atttatgggc agggataagg aatttaccaa taaagcaggt ttaataaact ggaaaaagca
    11041 aaatgaagca gcagcaaaaa ttttggctga atataacttt agtcatatta agccaacgga
    11101 catccttagt gatttaggtt ttgaagaaag aaaacaagtt gaaattgtga gagctctttc
    11161 tgttaaacca aaaatattgc tggttgatga gacttcagcg gctgtttcta aaaaatcagt
    11221 agaaattctt tataagttgt tacgtgaaca aagagacaaa ggtactgcag taatatatat
    11281 ttcacacttt attgatgaag tttatgagct gtgtgacagg gtaagcgtat taagagatgg
    11341 taagttagtg gctaaaatgg ttgtaaaaga tactactcct gaaatgatta tcaataatat
    11401 ggttggcagg gatatttcag gagaaagcta tagaagtgat aacaatgatt caattgggga
    11461 tgttatgtta gaagtaaaaa attttactaa agaaggtgtt tttgaagata tcaatttaac
    11521 cattagatct ggagaaattg taggtattgc aggtattggt ggttgtggtt cagatgaatt
    11581 tggacgggtt cttttcggat atgaacagcc aacaagtggc gaaatgattt ataaaggtaa
    11641 gaatggtcac atgaaatcac ctttagaagc aataaataat gggatagcat atattccaaa
    11701 agacagggat ttggaagggt tatttttaat atatgatttg gttatgaaca tctccgctgc
    11761 taatttaaag aaaatgcata agagaggaat tattaataat aaattggaag ttcaagtagc
    11821 aaaagattcc attaaaagac ttaagatcaa gacgccatct gaacggacgg ctgtatcaaa
    11881 tttaagcggt ggtaaccgtc aaaaagtatc tatagcaaaa tgggttgcca atgaatccag
    11941 tttattaatc gttaattcgc ctacgagagg agtagacgta ggtgctaaat atgaaatata
    12001 taagatttta gaatccctga aaaatgaagg gaaagccatt ttactaattt ctgatgaatt
    12061 accggaattg ttgggcatgt gtgacagaat ttatagtttc cgcaaaggaa gaaattccgg
    12121 tgaattcacc aggggtgtga attttacgga agaagctgtt gttgcaacca tggtctaaaa
    12181 caattttaag ggaaaggaaa ttgttatatg ggaatgacga atacaaaaaa taaagcagag
    12241 cttataaaag acaaattaaa aaagaatatc tctttaacag cttttatcat tctgtttatc
    12301 tttttctcat tgacaaccga tgggaaattt ttgacatcaa acaatataca aacgcttttg
    12361 ggggcgagta ttgacctttt gatttgtgcc ttaggggcta catttatcat catgttaggt
    12421 agtatcgatt tgtcatcagg ttctatgatt agcctttgtg gtgttattgc tgccaagatt
    12481 tatgagacaa caacaagtac attggctgca gttctggctg ctattgcagt aagtgttcta
    12541 ttttatggaa taatgggtgt agctcataca agacttcagg ttccaacctt tattgtaacc
    12601 ctgggtttct tatctattgc aagagcagtc gtaacgatga taagctcagg tactataaca
    12661 atgataccct ttgacagtcc ttttaaaaaa tattttggat taaaaccttg gataatcata
    12721 cttggactgg tcatgtttat cattgtatgg ctcatcgaaa gatatacgtt atttggaaga
    12781 tatacccgat taattggcgg tgatgagagg gtagcaaagt taagcggtat aaacgtgaat
    12841 aaacaaaaag ttttagtgtt cttatttgca ggcttactta ctgctattgg cggtgttgtt
    12901 atggcagcaa gaatcggttc aggatctcct gctatcggtc agggttatga gctaaacgtt
    12961 atatctgcag ttgtacttgg tggaacgtct cagcgtggtg gtatcggtgg tgtgagagga
    13021 acattaattg gtgcccttac cctggcagta ttgtccaatg gtttagttct tttaggaatg
    13081 ccttcagaag ttcaattatt tatcaaaggt attgttttaa tccttgcggt ttatgtgact
    13141 cttgaaagaa atcctaatgc cataacgaaa taaaacaaat gcttgaaaag gaaggagggg
    13201 gagaatgaac ttaaatacaa aaattgacat tataaagaat tgtaaagaac aaattttaaa
    13261 agaagcagaa gctttaaata gagtagcaaa acaagtggat gaggcatata ttgaagcttg
    13321 tgaagcaatt ctaaaatgtg aaggaagagt tatagtaaca ggattgggga aaaccggtca
    13381 catcggtaaa aagctggcag ctactttagc aagtttaggt attccgtcat tttttgtaca
    13441 cagttgcgag tctctgcatg gtgacatggg gatgattact aaagatgatg ttgtcatcat
    13501 gatttcgaat agtgggaagt cctcagaaat cttaagcatg attccagctt taaagattat
    13561 tggtccaaca actatttcaa ttaccagaga taaaaattct cctttagcta gagctacgga
    13621 tattaagatt ctttgtgatg caggagaaga aattgatcat ttaggtttag caccaacagc
    13681 aagcagtaca gcagcccttg ctattggaga tgctattgcg acagtggtat cctggataaa
    13741 aggttttcag cgggaagatt ttgcattaag tcatccaggc ggtgccttag gccagcaatt
    13801 aataaaagaa tacactgaaa gaatttcaca atagattttg aaagaagagc ggtgggattt
    13861 tagccaacca ccgctcattt aaaaaagatg aaactgtaat ataaaaagtt aggacgggaa
    13921 ataacgtgaa aaaaatgaat aaaaaacttt tgtatacata ctgtatttta atgatgttat
    13981 ttgttatttc tctttgcaat acaactcaag gcataatatt atcggattat attgtttatt
    14041 atgccttaga atcttcaaaa cagggattag tgagtgcttt tcaaagcact ggtaatatta
    14101 tcggacttat aataattatt ttattatcag ggaaatataa gaaaagtact atgctggcaa
    14161 tttctgcggc actgatacct gttatttttt ttatcatagg taataaacct gctttattta
    14221 ttttattatt aagctatgca ttttttggga tagtttttgc tctttgggat tctttgggtt
    14281 cgtcattaat ggctgacatt catacaataa attcatcacg atatatgaac ttattacatg
    14341 ggttttttgc tttaggcggt attataggac ctttaatttt tcaatcattg ggaacggaag
    14401 gctttagttg ggaccagatg ttaaggttgg caggcataat atctttagtg gcagtttttc
    14461 tttttacatt agggctagta tctgcaaaaa aacttattat tgaaaacaaa gacaacagtt
    14521 cagatatatt gattacagat attaaaacat tttttcgtga aaaaagaaat agaattatac
    14581 ttatttctgc ttttttatac ggagcccatc aaattggaat aaccgtttgg atgaccagat
    14641 atatttctga atatctgggg acacctcagt ggggaccggc tgcactatcg cttttttggg
    14701 tgggtatagc tatatcaaga ttagttgcaa gccgcttgtc actcgaacca gctaaatgga
    14761 ttttctttgg gcatttgatt agcggaattg tcatagctat aggagttctt tcggaaaatg
    14821 gttttataat gtttatatgc tgtggtttat caggtctggc agaaggggca attttaccga
    14881 tgactttgga tatggcctgt aattggagaa aaggtaaatc ttctttaggc agttccatga
    14941 ttctttttgt acattatgtc ggatttattg ttacaccacc tttaattggt gcgttaatct
    15001 ttacttatgg cataaaagtt ggtatgatta tgccagcgat tctttctatc gtagctactt
    15061 tatttacata ttttcttacc aggaaaggaa gagaaaggaa tgtatgattt aacagaaaat
    15121 tacttaaaat tacaaaatgg gagtgatatc agaggcattg ctttagaggg cgtaccaggg
    15181 gaaaatgtca atttaactcc agaaatagca agaaatataa catcagcttt tattacttat
    15241 ttatctaaaa aagaaaataa gtctgcagaa agcttggaaa ttgcagttgg acatgattca
    15301 aggatttctg ctagcctgct gaaagcaggt gtattagaag gaattcagaa tatgggttca
    15361 acagcttatg attgttccct tgcatcaaca ccatctatgt ttatgtctac cgtactagag
    15421 ggtcttgaat ttgatggggg aattatgatt acagccagtc atcttcctta caatcgtaat
    15481 ggtatgaaat tctttacaaa agatggctgt ctggaaagca aagaaatcag ggaagtgttg
    15541 gaaattgcga tagaaatcgc aaaaaaagag cccgggaaag ctgaacatat aaaagaagtg
    15601 cagaaattag atttactttc ttattatagt gactatctca aaaaagttat taaaaaagag
    15661 gttggaagtg aagatgagga taagccttta aaaggacttc atattgtggt agatgcttcc
    15721 aatggttcag gtggattttt tgttaagaaa gtactggaac ctctcggagc ggatacaaca
    15781 ggcagtgctt gtctggaacc tgatggtact ttccctgttc atgaacctaa tccggaaaac
    15841 agacaagcta tggaatatat taagaaaata acattagaac atcatgcaga tttaggcatt
    15901 atttttgata ccgatgtaga tagaatgggc gccgtatttt ccaatggaaa agaaatcaat
    15961 aaaaatgcta ttgtagccct tatgtcagct attgtagcgt cacaacatcc caatacgacg
    16021 attgtaacgg attcagtgac ttcagaacaa cttacagact tcctggaaaa caaattaaac
    16081 ttaaaacacc atcgttttaa aagaggttat aaaaacgtaa ttaatgaagc aattcgttta
    16141 aatcaggaag gtatagaaac ccatttagcc atagaaactt ccggacatgg tgcattaaaa
    16201 gaaaattact tcttagatga tggtgcctat atggcggtta aaattatttg tgaattagta
    16261 aaatgtaaaa gacaagggaa ggcgcttgaa gatttattag aaggtcttaa agaaccagaa
    16321 gatagtatcg aaatcagaat tcctataaaa gttgataatt ttaaagaata tggtcaagaa
    16381 gttcttgagg aatttaagaa atttgctggt gaaaatgata aatttaattt ggttaaaaac
    16441 aattatgaag gtgtaagaat tacatttaac gatgatgaag tttcaggttg gatgctgtta
    16501 agaatgtcct tacatgatcc tatcttacca atgaatattg agaccgataa taagggtggc
    16561 gtagcgattg ctcttaaaag attagctcct ttctttgaaa aatttgatgg actagatatt
    16621 tcagtactca gcaaataaaa atggtttaat gcagaaaata aacgattgaa aaataaatta
    16681 tacaataaat cattcaatgt gtgatatatt attaatgtaa cttattaaat aacaacaaag
    16741 gagagcaaaa gcatggcgtt agtaacgaca aaagaaatgt ttaaaaaagc ttataaaggt
    16801 ggctatgcaa taggtgcatt taacgtaaac aatatggaaa ttattcaggg cataacggaa
    16861 gctgctcagg aagaaaattc gcccgtgatt ttacaagttt ctgcaggtgc caggagatat
    16921 gcaaaacaca attatttaat gaaattagtt gaagcagctg ttatggaagt agatattcct
    16981 attgctttgc atttagacca tggagaggat tttgaaattt gtaagtcttg cattgatggc
    17041 ggatttactt ctgttatgat tgatggttca agacacagct ttgaagaaaa tattaagctg
    17101 accagaaaag tagttgaata tgctcacgct catggggtag tggtagaagg tgagttggga
    17161 gtgcttgccg gcatagaaga tgatgtcaat gtatctgtag aaaactcatc ttatacaaat
    17221 cctgatgagg ttgaggaatt tgttgaaaga actggtgtgg attctctggc cattgcaatt
    17281 ggcacaagtc atggagccta taaatttaaa ccaggacaaa aggtagagct tcgctttgac
    17341 attttagaag aaatacaaaa gcgtcttccg gatttcccaa tcgtattaca tggtgcttct
    17401 tcggttaatc aggaatatgt ggaaatgatt aaacaatatg gaggccaaat ggaggatgcg
    17461 atgggtgttc ctgaagaaat gttaagaaag gctgctaaat ctgctgtatg caagattaat
    17521 attgattcag atatccgcct tgcgatgaca gcagccataa gaaagtatat tatggaacat
    17581 cctgaagatt ttgacccaag gcagtattta aaaccagcaa gagaagcagt taaggaatta
    17641 gttaaacata agatccgtaa tgtattaggt tgttcaaatg cttgtaaata aaaaacaaca
    17701 ggtgaggtta tgaagaaaaa agcagttata tttgacatgg atggcttgat gattgatagc
    17761 gaaagggtaa cttatgatgg atatgtaaaa atatgcaggg aatttggtta tgaaatgact
    17821 ttagactttt ataaaacgct tttgggtctt aggatcaatg ttataagcca gaaattaaaa
    17881 gaacattttg ggaagttttt cccttcggaa gaaattatca ggagggttca catatatata
    17941 gaagagtatt ttcaggaaaa aggcgttcct ttaaaaacgg gcttgcagga aatccttcaa
    18001 tatttaaaaa gcagagatta taagctgatt gttgcgacat caaccgctcg aatcagagct
    18061 gagaaaatat taaaaaatgc cggtatttct caatactttg acggatatgt ctgtggagat
    18121 gaggtagtta atggtaaacc agataaagaa atttttataa aggcatgtga gaaggctaaa
    18181 gttaagccag aagaagttta tgttttggaa gactcagaaa tgggcatttt agcagcctcc
    18241 agagcaaaca tttcatgtat atgtgtacca gacatgaaat atccctctga agaaatggaa
    18301 gccaagacat ataaagttgc caataacctt ttagaagctt tagaaatcat taaggaagat
    18361 gaaaaaagat ttgagtgagg ggttgtgtgg acatgcttaa acaatttcat catctttgtc
    18421 tggtatcttc agattatgaa gcaaccataa aattttatgt ggacacttta ggttttacgc
    18481 tttaccgtga aagtttttca aataactgga atggtagaaa attagaattg tattataatg
    18541 gtcaatatat gttagaaatt tttgttgtag aaaaggcaaa aagaagagga caaaatcctg
    18601 gagaagtata tgcaggactt aatcattttt cttttttagt ggatgatgtc gaagcatttg
    18661 tcagaaaact tcacgatgat aatattgaaa cttcggaagt taaactggat aaaaacacag
    18721 gtaaaaaata tgcatttttt tacgatcctg atgggacaaa acttgaaata tatgaatcat
    18781 aaacggatat ttttaaattt aatttgctga taaatatctt ataagggagt tgtttatttt
    18841 ttagcaagaa tatattataa attttaggag gaatacagtg ataaaattag caccatctat
    18901 tttatctgca gactttgcta atttgggaag agacgtcaga gcccttcatg atgccggtgt
    18961 ggattatatt catattgaca ttatggatgg tggttttgta ccagtgatta cctttggttc
    19021 tcaactggta aaagccatcc ggaaatatag cgataaagtt tttgatgtcc atttaatggt
    19081 taataaccca gatgctcata ttaaaaattt tgtagaagca ggtgcagata ttatttcagt
    19141 acatgcagaa agttgtgtcc atttgcatag gacaattcag tgtattaaaa attatggctt
    19201 aaaggctgga gtggttttaa atccttctac tcccctttca agtatagaat atatccttga
    19261 agatgtagat atggtattaa ttatgtctgt gaatcccggg tacggggggc agaaatttat
    19321 tgacagtgct attcgaaaaa tatctgccct cagggaaatg attaatagca gggggcttaa
    19381 tactgacata gaagtagatg gcgggattac atttgaaaat gttcaggaag ttatccgggc
    19441 aggtgcaaat gtaattgtag caggttcgtc agtatttaaa ggtgatatcc ataaaaacat
    19501 caaagaattt aatagattat ttaaggaggt aaactaatgg ataaactaga gcttcagaaa
    19561 aaagcaaata agattcgcca aaacattatt cgatctattc atagtgccaa agccgggcac
    19621 ccaggcggtt ctttatcatc ggcggatatt ttaacttatc tttattttgt ggaattaaaa
    19681 aatattgatc caaaagatcc taaaaagtca gacagagacc gctttgttct ttccaagggt
    19741 catgcagctc ctgcatatta tgcgacatta gctgaaaaag gatattttga tgaaaaagat
    19801 ttaacaagcc ttcgtcaaat aggttctcat cttcaaggcc atccggatat gaaaggaact
    19861 cctggtgtag acatgagcag cggttcttta ggacaaggtg tttcagcagc ggttggtatg
    19921 gccatttcgg caaaaataag taatgaaaat tatcgtgtat atgcccttct tggagatggt
    19981 gaaattcagg aaggacaagt ttgggaagca gctatgctgg ctggccatag gaaattagac
    20041 aatcttgttg ttatagtgga taacaataat ttacaaattg atggaacagt cgaagaagta
    20101 tgttctccat atccaataga taaaaaattt gaggcattta acttccatgt tattcatgca
    20161 gatgctcatg attttgattc tttagaagct gcatttaatg aagctaaaca agtaaaaggt
    20221 aagccaacag caattattgc aaaatctgtt aaaggaaaag gcgtatcctt tatggaaaat
    20281 aaagtgtcct ggcatggcgt agcccccaat gacgaagaat ttgaaaaagc tatggaagaa
    20341 ttaaagaaag caggtgaagc attatgtcag aagtaaaaaa gattgcaacc agagaaagtt
    20401 atgcaagtgc tttagtggaa ttagggaaaa aatatgataa tgttgttgta ctggacgctg
    20461 atttagcggg tgcaacaaaa acagtaaaat ttcagaaaga gtttcctgaa agatttatag
    20521 attgtggtat tgctgaatca aatatgatgg gggttgcagc gggaatagca tcaacaggga
    20581 aagttccctt tgcaagcacc tttgcaatgt ttgcagcagg acgtgctttt gaacaagtga
    20641 ggaactccat cggatataca aagttaaacg ttaaaattgg agcaactcat gcagggatat
    20701 ctgtaggaga agacggggca agtcatcaat gtaatgaaga tattgcttta atgagaacca
    20761 ttcctggaat ggtcatcatc aacccttctg atgatatcga agcaaaagca gctgtatttg
    20821 cagcttatga acattatgga cctgtatata tgcgctttgg aagattagcg gttcctgtta
    20881 ttaacgataa ccctgattat aaatttgagt taggaaaagg gattaccctt cgtgaaggaa
    20941 atgacctgac cattattgca acaggacttt gtgtaagtgc agctttagaa gcagccgacc
    21001 aattaaaagc tgaaggaatt aatgcaagag tgattaacat tcatactatt aaacctatcg
    21061 atgaagatat tattattaaa gcagcaaaag aaacaggaaa gattgttgtt gtagaagaac
    21121 attcaattat tggtggctta ggaagtgctg tctcagaagt tttaagtgaa aactatccta
    21181 ctttggttaa gaggataggt atacgggatg ttttcggcga gtctggtcct gctgtacaat
    21241 tgcttaaaaa atatggttta gatgcagaag gtatttataa acaagtaaaa gaatttttac
    21301 aataatatcg gttttgttga ataagtagtt ttattagatt aattaacagg attgtaaata
    21361 attaatatta attatttaca atcctttcat tctaagattc aataaggctt aataattaca
    21421 tacattacgg tattataaat ttgtgtaata atgggtccga atttgactta ctatttagaa
    21481 tataaatcat tttatttcaa taaattatta ttcataatgc attaattatt taaaaattta
    21541 tttttgagct tgatttatta tttgggagga accactgtgg gtat
//
LOCUS       DAOSUD010000036        21074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1638_length_21074_cov_25.772967, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000036 DAOSUD010000000
VERSION     DAOSUD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 21074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21074
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1638_length_21074_cov_25.772967"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1694
                     /gene="tig"
                     /locus_tag="PLG49_06090"
     CDS             408..1694
                     /gene="tig"
                     /locus_tag="PLG49_06090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOQ16929.1"
                     /translation="MNSQLERLDNNQVKLTIEVAAERFEEGMNYAFNKNKKYISVPGF
                     RKGKAPRHLVEKMYGPEIFYEDAVNYIIPGEYEKAVEEHDLDVVSRPEFDVEKIGKGE
                     NLVFTAVVTVKPEVKLGQYKEIELPKINVEVTDEEINEELKKVQEKNSRLFTVTDRAV
                     KDGDEVVIDFEGFVDGEAFEGGKATDYTLKIGSRTFIDNFEEQLIGKNPGEQLDVNVT
                     FPEDYAQKSLAGKAAVFKVEIKGIKEKELPALDDEFAKDVSEFDTLDEYKQDIKEKLL
                     EQKESRARREKEKDLLEKIIANAEMDVPGVMIENKIDQMLQDFEMRMAYQGLPFEQYL
                     QFTNQTIDALRDKFRPEAEFQVKAGLVLEQIAKEENIEVSDEELEEEYKKLAEQYNME
                     LDKIKESMGESGSKALKEDIKIRKAVDLVVESAVEK"
     gene            1720..2319
                     /gene="clpP"
                     /locus_tag="PLG49_06095"
     CDS             1720..2319
                     /gene="clpP"
                     /locus_tag="PLG49_06095"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOQ16930.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIIFLGEEINNVTANLV
                     VAQLLFLEAEDPDKDIYLYINSPGGSVTAGMAIYDTMQYIKADVSTICIGMAASMGAF
                     LLSGGAKGKRFAVPNAEVMIHQPLGGASGQATDIQIHAEHILRIKKRLNKLLSENTGQ
                     PLEKIERDVERDYFMTAEEAKAYGLIDEIISSRKEIEKK"
     gene            2331..3626
                     /gene="clpX"
                     /locus_tag="PLG49_06100"
     CDS             2331..3626
                     /gene="clpX"
                     /locus_tag="PLG49_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOQ16931.1"
                     /translation="MAGRYDEKKQLKCSFCGKTQEQVRRLIAGPGVYICDECIELCFE
                     IIEEEFNELPESNELSDIPTPKEIKEHLDEYVVGQERAKRALAVAVYNHYKRIYSENG
                     YDDDVELQKSNILMLGPTGSGKTLLAQTLAKMMNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYDIERAEHGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFICGGAFDGIEKIIEKRIGKSGLGFGAEVKSKK
                     EKNIGEVLTHLLPQDLLKYGLIPEFIGRVPVIVSLDDLDEDALVRILTEPRNALIKQY
                     KKLFEMDDVILEFEEEAIRLIAKKAIQRETGARGLRAILEELMTDVMYEIPSTKNVEK
                     CIITKATIEKKEMPQLIINENKQPIKKKRKKRANRGETA"
     gene            3733..5400
                     /gene="lonB"
                     /locus_tag="PLG49_06105"
     CDS             3733..5400
                     /gene="lonB"
                     /locus_tag="PLG49_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOQ16932.1"
                     /translation="MSSVLIIIQFVFSVIIGIYFLVLLKNQQTTKISIGKESKKELEK
                     LKNLRLIHLTEPLSEKTRPNSFKEIIGQDDGLRALKAALCGPNPQHVIIYGPPGIGKT
                     AAARAALNEAKKIPFSPFKEDAPFIEMDATTLRFDERGIADPLIGSVHDPIYQGAGAY
                     GAAGIPQPKPGAVTKAHGGVLFIDEIGELHPIQMNKLLKVLEDRKVYLESAYYSSEDP
                     NIPSHIHDIFQNGLPADFRLIGATTRSPEEIPPAIRSRCTEIFFQPLTQEDVKQIVKN
                     AAKKGAFSLDEGVAELISTYANNGRDAVNILQTAGCVAIMENRSDITIKDIEWVIEFG
                     QYNKRLDKKIFSEKRIGSVNGLAVYGIRMGALLEIEVTAQKAEEKSKGSLKVTGIIEE
                     EEFNKNNGKMKRRSTAFASVENVLTVMKNVFDIPYKDYDIHINFPGGMPVDGPSAGVA
                     IFIAVYSAILKKPVSAKIAMTGELSIRGNIHPVGGVHAKINAAREAGIEKVFIPLDNW
                     QETYKNYAIEVIAVKNIEELVKQVFEKQNDDIIVKTKSAEVLTAQGI"
     gene            5618..6064
                     /locus_tag="PLG49_06110"
     CDS             5618..6064
                     /locus_tag="PLG49_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16933.1"
                     /translation="MVEILLLQKQAYISEAELYGDFNMLPLVETLDEIEQEFSTYLFL
                     KAIINGVIIGSVRAYQEGDTCYIGRLVVHPKFQRQGIGTRLIREIESIFRTCRRYEIF
                     TGHKSIRNINLYKKLGYKEFQVKEVSPNLQYIYMEKINENLQSNIV"
     gene            6262..8694
                     /gene="lon"
                     /locus_tag="PLG49_06115"
     CDS             6262..8694
                     /gene="lon"
                     /locus_tag="PLG49_06115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOQ16934.1"
                     /translation="MTRKTDNIIQIPLVALRGLTVFPKMILHFDVARQKSIKALEESM
                     VKGEPIFLVAQKEAEIEEPDIEDLYTVGTISHIKQILKLPNNAIRVLVEGHERGRIIS
                     FKQKDPFFLVYVEKDNKTRKMNTDSDNLNVKALLRLAAETLEDYARINNRISPDTLNS
                     IISMEKPGEMADAIISNIHLKLEIKQQVLSEFDEENRLMMAIEILKSEIEIMEVQKEI
                     LEKVKKKIDKSQREYYLREQLKIIQNELGDKDGIQAEVDEYRAKLERLSPPQEVRERI
                     EKELDKMIKMPSSSAESAVVRNYIQWLLDLPWTQETRESISIDKAEKILEKDHYGLEQ
                     VKERILEYLSIRALSPEADSPIICLVGPPGVGKTSIAHSIAKALNRKYVRISLGGVRD
                     EAEIRGHRRTYVGALPGKIIQAMKNAKSKNPLMLLDEIDKMSSDFRGDPASALLEVLD
                     SEQNSAFVDHYIDMPFDLSKVLFLTTANTLDTIPKPLLDRMEVIRISGYTTEEKFHIA
                     NNHLMDKQLKKNGLKKSQIRIDKKAIESIIIYYTKEAGVRQLERMIGQLCRKVAKEII
                     KENKKMVRITESNLEKYLGKKKYRLNKIIEEAEVGIARGLAWTPAGGDTLSIEVNTMK
                     GTGKFELTGQLGEVMKESAKAAISYIRSKAEDFGIDADFYKTNDIHIHVPEGAVPKDG
                     PSAGITMATAMISALTNMPVNNYVAMTGEITLRGRVLPVGGLKEKILAAKAAGIKKVL
                     IPAENEVDLQEIPDSVKNNLEIVPVKTMNEVLMHALINPIEKKERADNNEFTISKEIF
                     NSQDSNEMIHPS"
     gene            8726..9319
                     /gene="yihA"
                     /locus_tag="PLG49_06120"
     CDS             8726..9319
                     /gene="yihA"
                     /locus_tag="PLG49_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOQ16935.1"
                     /translation="MEVNNVKLEHVCGNASQFPDTNLNEIAFAGKSNVGKSSLINALV
                     NRKSLARTSAQPGKTQTINFYNVEGKLYFVDLPGYGYAKVSAQERERWGKFIESYLVN
                     RKQLKQVVLLIDIRHKPGENDKLMYEWLRYYQSNILVVATKADKIKRSQYQKHLSIIR
                     KELGMDSSDILIPFSSVTKQGKKEIWDLFEQWLLIEE"
     gene            9420..9629
                     /locus_tag="PLG49_06125"
     CDS             9420..9629
                     /locus_tag="PLG49_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ16936.1"
                     /translation="MKKKLCLSNRDKKFAGVCGGIAEFFDIDATLVRIFWALLTLMSS
                     IIPGIILYLICWAIMPMPDDYKDMR"
     gene            9792..10346
                     /locus_tag="PLG49_06130"
     CDS             9792..10346
                     /locus_tag="PLG49_06130"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOQ16937.1"
                     /translation="MKKTKAILGLPIISISTAENVGKVNNLIVNGADGTVDYILVETG
                     DKAINSLVISTRDIIGVGEYATTIDNEGVMIDISSVPDAIDLLQKNVQIKNTSILTKN
                     GNLIGKSGDFFVDEDFNIAALEFIEEGNSDEVKLILRKDVITFGKNLIVVEEDIEDRI
                     LDSEDELFSDEKSSFFIDPMKKAL"
     gene            10421..11371
                     /locus_tag="PLG49_06135"
     CDS             10421..11371
                     /locus_tag="PLG49_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16938.1"
                     /translation="MMTMSFEDEDSFDDNLIKDESLEEVKQADIESVKTAIAEAIAAA
                     EAEIKVEEEVLEEKTDVDIAVEEAELAVEEAVVAAESVAGLIEPVEDEVEEDIKEENT
                     EENTEEFVEEGSAKETESEEAAEQTEEQAEIEEEIVAANIMSEPYNEEETEKLSEKEF
                     FYIDMDEDMMDEDQIETINLDENILDFEDEDSQLEFDFNNNDLDFNYVSDDLPNEVFY
                     SFGKEKDKDKEDEEDGFMSRIMKKMQEAGNPIDLERNKQLDGVFEQKQIEFLTGKTIT
                     KDIVTDSGKVIAKAGELISAETITNAKKNGKLIDVIMNCK"
     gene            11499..12065
                     /locus_tag="PLG49_06140"
     CDS             11499..12065
                     /locus_tag="PLG49_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOQ16939.1"
                     /translation="MKKSSTLIGLTVVSLATAEKVGNVSSYLVNAEKGRVDYLVIETD
                     HEMMNSFVISTKDVLRAGNNAVMVENEDVMLDNRSVADAIKLFQKNIQIKDTNILTDK
                     GNYLGRTGDFYIHEERFDITGLELIPSDGGASKIIPSKYVIIYGKNTIIVKDEAVHNL
                     LNSEDEFEDEYQEVEQVSFFMGPMKKTA"
     gene            12083..13225
                     /locus_tag="PLG49_06145"
     CDS             12083..13225
                     /locus_tag="PLG49_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16940.1"
                     /translation="MDNLDNELSFEDRETQIILNDVIIDEDEELTNHLSFNSPITDEF
                     EQNENELSEDFSSLEEQDSNLTFDSTVLEDENIDLNQNEISLEEEAITLDQEVQSELH
                     FEEETDTMDEPLLEDDETKMTLEEMIQETLQREGLIAPGEKFSLDDVEPVDIDYDLLL
                     QQQNSFNFGGDNQSESDLLDDDDDDDDSMDLGNDFADDSDDDDEEVKASNLLFADEEK
                     SANNKLSDSLLDFDSNDFELSFDGEKSLEDTEDADNASYSLLMNDENEEDDINDDYAS
                     SFGSFFDDIGVEKNSEQELIDKVLKDIQAADSGAGYDELGMEFSKKDSVFEKKQTKYL
                     TGKRVTKDIRNEHGEIIAKAGDVIDEKIIKNAKRQGKLIELIMNSN"
     gene            13403..13645
                     /locus_tag="PLG49_06150"
     CDS             13403..13645
                     /locus_tag="PLG49_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16941.1"
                     /translation="MKFERQWIPNYTELEDEELLKQIELLRKELEDTKNWLEESANEK
                     GPMAYMNKRMAKLAHSLAREKYRLYTEEAQKRGILD"
     gene            complement(13650..14189)
                     /locus_tag="PLG49_06155"
     CDS             complement(13650..14189)
                     /locus_tag="PLG49_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOQ16942.1"
                     /translation="MFDSTRELTTNKLILLYLLHKIQIPISHAQITDFIMEKGYIDYF
                     SLQQYLTELSVSNLVIKEQDNHTTRYTIADKGINTLEYFSNRIPASIKSEIDGYIQKN
                     RRRLKNELEVSAEYFPNASNEYIVKCTIKEDERSLIHLEINTASKEQAKNICSNWENN
                     AQALYQSILASLTNPTGQQ"
     gene            complement(14216..15145)
                     /locus_tag="PLG49_06160"
     CDS             complement(14216..15145)
                     /locus_tag="PLG49_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414973.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOQ16943.1"
                     /translation="MNWDGIAYHSLNYHLRKKFNEKVFKISLDGGFTCPNRDGSISDN
                     GCIFCSGRGSGDFIPAADLSLFEQFMKGKELLEKKWPAGKYIAYFQAFTNTYAPVDIL
                     RKKYEEALSFPGVVGLAIATRPDCLGHDVLDLLDEINKKTYLWVELGLQTIHEKTASL
                     IHRGYSLSCFNEAVYNLNERKIESVVHLILGLPGENKEDMLSSVKYISMQPVQGVKLH
                     LLHILKNTPLYQMYLKNPFKVLEKDEYIDLIIDCLELLPPDMVIHRLTGDGSKDLLIA
                     PLWSKNKKAVLNGIHKRLKERNTWQGKFYRNHI"
     gene            15322..15996
                     /locus_tag="PLG49_06165"
     CDS             15322..15996
                     /locus_tag="PLG49_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ16944.1"
                     /translation="MDISVIIPAYNEEGRIGKVLSSLIDLECINEIIVVNDGSDDGTS
                     EEAKKYPVKVIDIAQNIGKAGAMKKGLIGSKGEWILFLDADLMGLSREHIQAMIDCAK
                     KQDADMVIGLFTKGNLITDISHKIAPFLSGQRLIKRAIIENIDFDETIAYGIEIYLTN
                     YFRKKGYYVKKVFMPNLYHIPKEIKRGFIKGKKEKLKMYCDIIFTVIQLYLIPKWLKK
                     LYKCNI"
     gene            complement(16000..16431)
                     /locus_tag="PLG49_06170"
     CDS             complement(16000..16431)
                     /locus_tag="PLG49_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16945.1"
                     /translation="MSNILSREELFFELDNLRDLIQNFDYSELENVTFLNLESLFTYI
                     AEVEDNPFQRQYEALEAALDKLEPFIPFTSRERAREFLIKMNQVETNEEIEWLKEEFS
                     NKVRNDFVNMIRLIDSEEEWLHLKEICEILRQSKEYYHTLI"
     gene            16730..17563
                     /locus_tag="PLG49_06175"
     CDS             16730..17563
                     /locus_tag="PLG49_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16946.1"
                     /translation="MQKKFLNKKMIFTVFFLVVISFSLGLCFQNASASSFLIFLNDKQ
                     DSIKCYPKGGYIKYKGEKYYLVTETDKVIIDMTAADIDNNMKEDILIIEGKKDSEYGD
                     NLIIYETEFLPGKIKLTEKYRNNLTAINPWTIKICNIDNDSEPEIFIGTYKETVYYPG
                     LENRPFFFNYRDNKLVKKWTGSKLRAPFKDICFGDLNDNGSDELIVIEELEEDKNIIS
                     VYYWFGFGFILYAESSVYKNIEFVETEIIDGKLLIKTDQGYLELSADKSESGVYLLKE
                     R"
     gene            17570..19822
                     /locus_tag="PLG49_06180"
     CDS             17570..19822
                     /locus_tag="PLG49_06180"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOQ16947.1"
                     /translation="MSKKIIAVFLLMTILLTACSSSRTGMSAGANSGKSISGKYLLKH
                     ADTSAYNNETVPFTETSYKPSIPQYSLNPDLSNVENAEQFEGLTQEQINMLVNNGFIV
                     LNPNRNKAHYYLKMYDIYEENEYKNVPNFITVDVALHIYHKFYDETLKSIEKEHLYTA
                     LQELTENMLNKSIALYKEETSKDTKELLENIMVYFSVANKLINNSYGDIPEELLPVAE
                     EEISLIDAAKGYIKSPLLDYDLNYEEFTVRGHYTGDEILEPYFKTMIWYGIGAYPLVD
                     ENGKIDYESAQKALAITYILLLDNDGSNDIALWDKIYSPTNLFIGQSDDIGILEIKEL
                     LVNVYGDDVNLEKITDKKYKKKLAEEIEKLPSPQIEYKLVTGKVDTPSDKQFRFMGQR
                     YTLDANIMQELMFPIIRPVPTGLDVAGAFGSERAEALAKQYCLNDLDEKEYSSKLTEL
                     KNKVKALKLEDWQKNLYNGWLWVLESLWTKDYKKEGYPVFMQNQAWEDKTISSALGSY
                     AELKHDTVLYSKSPVAEMGGGGELQEKYPHYVEPAVEVYDRLLWLVRYSKVNLEKRGL
                     LSENSADVLKNIEDLYELFRVCSVKELENKPLSEEEYEALKYIGGRLEWIDDQLSNQY
                     NQPLSPAVVSDVAGLADVGMFVEIGTGLPNDIIVAIYDNGKIYLARGAVYSYYEFLSD
                     KPLTDEEWHDIIGVKKVVDGEWEFEKVDPDSIGNEAEFRPPWVNSFKSFEENKVNIPD
                     IEYIIPDSEE"
     gene            complement(19869..20564)
                     /locus_tag="PLG49_06185"
     CDS             complement(19869..20564)
                     /locus_tag="PLG49_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ16948.1"
                     /translation="MLNSYYEFAWDINYCYPNSFVLRNKLNIKNKEELEKAERQITSI
                     KILDLKMNPLKGNLDFDHLLSIHKYIFIDIYPWAGMTRTVNISKGNPFCDFKYINDVA
                     SELFAQLHREHYLIGLNQKQVIERLAYYLGELNAIHPFREGNGRTQRLFIEYLGLVAG
                     YHVNFTKISSQEMIDASVDSFLKKYDKMNKLFEKAVTPISIEKQEEFINLLATKSSEY
                     KKIFYIWKEQINQ"
     gene            complement(20585..20767)
                     /locus_tag="PLG49_06190"
     CDS             complement(20585..20767)
                     /locus_tag="PLG49_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16949.1"
                     /translation="MKSSFYVVNPSQLVKEVKSTMAIEGFYLDKQEIDMLKKCAYGQV
                     SSSHLVKCLVKKYTKK"
BASE COUNT         7779 a         2719 c         4181 g         6395 t
ORIGIN      
        1 tatagaaatt tttgagataa aaattaattt tttgtataca attaacataa ttaaatataa
       61 ttaaattttt aaaagtttat ttatgatgga atgatattat taaagagaaa ttctctgata
      121 tcttgacgtt aattttttat tcagatataa ttcttatgtt aaggaaatca aattttaaaa
      181 gtaaatgagt agatttaaac agtagcttgt ggtataattt ttaaatggtt gacttgtcgt
      241 atagaaaatc aaagagtttc ttttaaaatt gatttgagag aagcagttta tatattatca
      301 tgggttcctt gcttctctat attttatatt tttatataag aaaactatgg atacacattg
      361 atgagtgtac atagattata atattgtgtt taaggaggac aaatgaaatg aattcacaat
      421 tagaacgatt agataataat caggtaaaat taaccattga agttgcggct gaaagatttg
      481 aagagggtat gaattatgct ttcaataaga ataaaaagta catttcagtt cctggctttc
      541 gtaaaggcaa ggctccaaga catttagtag aaaaaatgta tggtccggaa attttttatg
      601 aagacgctgt caattatata attcctgggg aatatgaaaa ggcagtagaa gaacatgatt
      661 tagatgttgt atcaagaccg gaatttgatg ttgaaaaaat tggcaagggt gaaaatttag
      721 tatttactgc tgtagtaacg gttaaaccag aagtaaaatt gggacagtat aaagaaattg
      781 aacttcctaa aataaatgtg gaagtaacag atgaagaaat caatgaagaa ttaaagaaag
      841 ttcaggaaaa gaactcccgc ctctttactg taacggacag agcagtaaaa gacggggatg
      901 aagttgttat tgattttgaa ggttttgttg acggagaagc ttttgaaggc ggaaaagcga
      961 ctgattatac tcttaaaatt ggttccagaa cttttataga taattttgaa gaacaattaa
     1021 ttggaaaaaa tccaggggaa caattagatg tcaatgtgac tttcccggaa gattatgctc
     1081 aaaagtcttt agcgggtaaa gccgctgtat ttaaagtaga aattaaaggc ataaaagaaa
     1141 aagaacttcc tgctcttgat gatgagtttg ctaaagatgt ttcagaattt gatacattag
     1201 atgaatacaa acaggatatt aaggaaaaac tcttagaaca aaaggaaagc agagcaagaa
     1261 gagaaaaaga aaaagatcta cttgaaaaga tcatagcaaa tgcagaaatg gatgttcctg
     1321 gagttatgat agaaaataag attgatcaaa tgcttcagga ttttgaaatg aggatggcat
     1381 atcagggact tccatttgag caatatcttc aatttacgaa tcaaacgata gatgcattga
     1441 gggataaatt ccgtccggaa gcagaattcc aggtaaaagc aggcttagta ttagagcaaa
     1501 ttgcaaaaga agaaaacatt gaagtgagtg atgaagaatt agaagaagaa tacaagaagc
     1561 tggcagaaca atataacatg gaattggata agattaaaga aagtatgggt gaatcaggaa
     1621 gcaaggcatt aaaggaggac ataaaaatcc gtaaagcagt tgatctggtt gttgaaagtg
     1681 cagtagaaaa gtaatatata aataaaagga ggaatggtta tgagtttggt acctattgtt
     1741 gttgagcaaa ccaacagggg tgaacggtct tatgacatat attccagact tttgaaggat
     1801 agaataatat ttcttgggga agaaataaat aatgtgactg ctaatttagt tgttgcacag
     1861 cttttgtttc tggaagcaga agatcctgat aaagatattt acttatatat caatagtcct
     1921 ggtggttctg tgactgctgg tatggccatt tatgatacga tgcaatatat taaagcagat
     1981 gtttcaacca tttgtattgg aatggctgca agcatgggag ctttcttact aagtggcggt
     2041 gcgaaaggaa aaagatttgc agtacctaat gcagaagtaa tgattcatca gcctcttggt
     2101 ggagcaagcg gtcaggctac ggacattcaa attcatgcgg aacatatttt aagaattaaa
     2161 aaacgcttaa ataagctttt aagtgaaaat accggccaac ctctggaaaa gatagaaaga
     2221 gatgttgaaa gagattattt catgactgct gaagaagcaa aagcttatgg attgatagat
     2281 gaaatcatta gttcaaggaa ggaaattgaa aagaaataag aggtgagtgt atggctggca
     2341 gatatgacga gaagaagcag cttaagtgtt ctttttgcgg aaagacccag gaacaggtaa
     2401 gaagattgat tgccggacca ggcgtatata tatgtgatga gtgtatcgaa ctttgttttg
     2461 aaatcattga ggaagaattt aatgaactcc ctgaaagcaa tgagttgtct gatattccta
     2521 ctcccaaaga aattaaggaa catttagatg agtatgttgt aggtcaggaa agggctaaaa
     2581 gagctttagc tgtagctgtt tataatcatt acaaaaggat ttattcagaa aatggctacg
     2641 atgatgatgt ggaattacaa aaaagtaata ttttaatgct cgggcccact ggttctggta
     2701 aaaccctttt agcacaaact ttagccaaaa tgatgaatgt accttttgct atagcagatg
     2761 ctacttcttt aactgaagca ggatatgtag gtgaagacgt agaaaatatt cttctaaagc
     2821 tgattcaagc tgcagattat gatattgaaa gagcagaaca tgggattatc tatatcgatg
     2881 agatagataa aatagcaaga aaatcagata acccttccat taccagggat gtaagtggtg
     2941 aaggggttca acaggccctt cttaaaattc tcgaaggcac tgtggcttct gttccgccgc
     3001 aaggtggcag aaaacatcct catcaggaat ttatacaaat tgacacgacc aatattctgt
     3061 ttatctgtgg aggcgctttt gacggcatag aaaaaattat agaaaagcgt attggaaaaa
     3121 gcggcttagg ttttggtgca gaggtaaaaa gtaaaaaaga gaaaaatatt ggggaagttt
     3181 taactcattt attaccccag gatttgctta aatatggttt aattccggaa tttataggtc
     3241 gtgttccagt gatcgtttcc ctggatgacc tggatgaaga tgctttagtt cgcatattga
     3301 ctgaacctag gaatgcactg ataaagcaat ataaaaagct ttttgaaatg gatgacgtta
     3361 ttttggaatt tgaagaggaa gcaatcaggt taattgctaa aaaagcaatt cagagggaaa
     3421 ctggtgcaag aggtctcagg gctatattag aagaattaat gacggatgtt atgtatgaaa
     3481 taccatctac taaaaatgta gaaaagtgta ttattacaaa ggccaccatt gagaaaaaag
     3541 aaatgccaca attaattatt aatgaaaata aacagcccat aaagaagaaa aggaagaaaa
     3601 gagcaaacag aggtgaaact gcttaagaaa aagatgcata aactgcatct tttttctttt
     3661 atctttgtaa taaaaagcct gctattgagc atactaaagc tatagtattt atgcacgata
     3721 ggaggccgtt aagtgagtag tgtattgatt atcatacaat ttgtattttc agtaattatt
     3781 ggaatctatt ttttagttct tttaaaaaac caacaaacaa cgaaaatatc cattgggaaa
     3841 gaatcaaaaa aagaactgga aaaattgaaa aacttaaggt taattcattt gacagaaccc
     3901 ttgtctgaaa aaacacggcc aaattctttt aaagaaataa taggtcagga tgacggattg
     3961 agggctttaa aagcagcttt atgtggacct aatccccaac atgttattat ttatggtcct
     4021 ccaggcatag gaaaaacagc tgctgccagg gcggcattaa atgaagccaa aaagattcct
     4081 ttttctcctt ttaaagaaga tgctcctttt attgaaatgg atgctaccac ccttagattt
     4141 gatgaaaggg gaattgcgga tccattaatt ggttctgttc atgatccaat ttaccaggga
     4201 gctggagctt atggagcagc agggatacct caaccaaagc ctggtgctgt gacgaaagcc
     4261 catggaggcg tattgttcat tgatgaaatt ggagaattac atccaattca aatgaataaa
     4321 cttttaaaag ttttagaaga cagaaaagtt tatttagaaa gcgcttatta ttcttctgag
     4381 gatcctaata tccccagtca tattcatgat atttttcaaa acgggctccc tgcagacttc
     4441 agactgatag gggcgacaac cagaagccca gaagagattc ccccagctat ccgttccaga
     4501 tgtacggaaa tattttttca gcccttaacc caggaagatg tgaagcaaat tgtaaaaaat
     4561 gcggcaaaaa aaggggcttt ttccctggat gaaggggttg cagaattaat ttctacttat
     4621 gcgaataatg gccgggacgc agtgaatatt ttacaaactg ctggctgcgt ggccataatg
     4681 gaaaacagat cagatattac aattaaggat attgaatggg ttatagaatt cggacagtat
     4741 aacaaacgat tagataaaaa gattttttca gaaaaaagaa ttggttcagt taacgggctg
     4801 gctgtctatg ggatcaggat gggcgctttg ctggaaatag aagtaactgc tcaaaaggca
     4861 gaagaaaaat ctaagggcag cttaaaagta acaggaatca tcgaagaaga agaatttaat
     4921 aaaaataatg gtaagatgaa aagaagaagt acagcttttg cttctgttga aaatgtttta
     4981 acagtaatga aaaatgtttt tgatattcca tataaggatt atgatattca tattaatttt
     5041 ccaggtggga tgcctgtaga tgggccttca gcgggagtcg caatatttat tgctgtctat
     5101 tcagcgatac ttaaaaaacc agtatcggct aagatagcaa tgacaggcga gctttctatc
     5161 cgtggtaata ttcatcctgt tgggggtgtt catgcaaaaa taaatgctgc aagagaagct
     5221 ggaattgaaa aagtatttat tcctttagat aattggcagg aaacatataa aaattatgcc
     5281 atagaagtga tagccgtaaa gaatatagaa gaactggtga aacaggtatt tgaaaagcaa
     5341 aatgatgata ttattgtaaa aacaaaatca gccgaggttt taacggcaca aggaatttga
     5401 ttgtacaatt ccttaaaaag agcatcattg taaaaagaaa agatattgat ggaaatttgt
     5461 aattatatat tgagaatata attttaaata tgatataatt tttaacatat aaaactaatg
     5521 taaaccaatg gagaaatact ttcattggtt tttaatatta ctatagacag acaggggggt
     5581 aatacttctg gttatcgagc aggcgcagtt aaaagatttg gtagagattc ttttacttca
     5641 aaaacaagcc tatattagtg aagcagagct atatggcgat ttcaatatgc tcccgctagt
     5701 tgaaacttta gatgaaattg agcaagagtt ttccacatat ttatttttaa aggcaattat
     5761 taatggggtt atcattggtt cagtaagggc ttatcaagag ggcgacactt gttatatagg
     5821 aagacttgta gtacatccaa aatttcaaag acaagggatt ggaaccagac ttatcaggga
     5881 aatagaatct atctttagaa cttgtagaag atatgaaatt tttactggtc acaaaagcat
     5941 tagaaatatt aatttataca aaaaattagg atataaagaa tttcaggtta aagaggtaag
     6001 tccaaatctc cagtatattt atatggaaaa aataaatgaa aatttacaaa gcaatattgt
     6061 ttaaaggagg cgtagagaag aagacggctc ctttttttgt gaaaaaagta gctggaaatt
     6121 agacgttgaa gattaaaaaa gaagaaagaa aaaagaaagt tggaattagg aaatggaaat
     6181 aaaatacata ataaaatctg atttgacaag tgtcgataaa ataagcataa ttactataga
     6241 cactagatgt ggggtgaaaa tatgacgaga aaaacggata atataataca aatacctttg
     6301 gttgcattaa gaggtcttac agtttttcca aaaatgatat tgcattttga tgtggctcgt
     6361 caaaaatcta ttaaagcttt agaagaatct atggtaaaag gagagcctat ttttcttgtt
     6421 gctcaaaaag aggctgaaat agaagaacca gatatagaag atttatatac agtcggaact
     6481 atctcccata ttaagcagat attaaagctg ccgaataatg cgattcgtgt cttggtggaa
     6541 ggacatgaaa ggggaagaat tataagtttt aagcaaaagg atcctttttt tctggtgtat
     6601 gtagaaaaag ataataaaac aagaaaaatg aatactgata gtgacaattt aaatgtaaaa
     6661 gcacttttaa gattagctgc ggaaacgctt gaagattatg ccagaataaa taacagaatt
     6721 tctccggata cacttaacag tattatttcg atggagaagc cgggggaaat ggcagatgca
     6781 attatatcca atatccattt aaaattagag attaaacaac aggttttaag tgaatttgat
     6841 gaagaaaatc ggttaatgat ggcaatagag atacttaaat ctgaaattga aatcatggaa
     6901 gtacaaaaag aaattttaga aaaagttaaa aagaaaattg ataaatctca aagagaatat
     6961 tatttgagag aacagttaaa aataattcaa aatgagttgg gagataagga cggaattcaa
     7021 gccgaggttg atgaatatag agcaaaactg gaaaggctta gtccgccaca agaggtaaga
     7081 gaaagaattg aaaaagagct tgataaaatg ataaaaatgc cttcaagttc agcagaaagt
     7141 gcagttgtaa gaaattatat tcagtggctg ttggaccttc cctggactca ggaaacgaga
     7201 gaaagtatca gcattgataa agctgaaaag atattagaaa aagaccacta tggtttagaa
     7261 caggttaaag aaagaattct ggagtattta tctataagag cgctttcgcc tgaggcagac
     7321 agccccatta tatgtctggt tggaccaccg ggcgtaggaa aaacttctat tgcgcattct
     7381 attgcaaaag ctcttaacag aaaatatgtt agaatttcac tcggaggcgt aagggatgag
     7441 gctgaaataa gagggcatcg cagaacttat gttggtgcac tcccgggcaa gattattcag
     7501 gctatgaaaa atgcaaaaag caaaaatcct ttaatgcttt tggatgaaat tgataagatg
     7561 agcagtgatt ttaggggaga tcctgcatct gcattattag aggttttgga ttcagagcaa
     7621 aattctgctt ttgtagatca ttatatagat atgccttttg atttatcaaa agttttattt
     7681 ttaacaacgg ccaatactct tgatacaatt cctaagccct tactggacag gatggaagtt
     7741 atccgcatat ctggttacac aacagaggaa aaattccata ttgcgaacaa tcatttaatg
     7801 gataagcagt tgaaaaagaa cgggcttaaa aaatcccaaa ttcgtatcga taaaaaagcc
     7861 attgaaagta ttatcattta ttatacgaaa gaagctggtg tcagacagtt agaaagaatg
     7921 ataggacaac tatgcagaaa agtagctaaa gaaatcatta aagaaaataa aaaaatggtc
     7981 cgcattaccg aatccaattt ggaaaagtat cttgggaaga agaagtacag actaaataaa
     8041 attattgaag aagcagaagt gggcatagca agaggacttg catggacacc tgcaggagga
     8101 gatacgcttt ctattgaagt caatacaatg aaagggacag gaaaattcga gttaacgggt
     8161 caattaggag aagttatgaa agagtcagca aaagcggcaa ttagttatat ccgttcaaag
     8221 gctgaagatt ttgggattga tgctgatttt tataaaacga atgacattca tattcatgtt
     8281 ccggaagggg ctgtccccaa agatggtcca tcagcaggaa ttacaatggc aacagctatg
     8341 atttctgcac tcacaaatat gcctgtgaat aattatgttg caatgactgg ggaaatcacc
     8401 cttagaggaa gagttttacc tgttggaggt ttaaaagaaa aaattcttgc tgctaaagca
     8461 gcagggataa aaaaagttct gattcctgca gagaatgagg tggatttgca ggagattcct
     8521 gattcggtta aaaataatct ggagattgtg cccgtaaaaa cgatgaatga agtcttaatg
     8581 catgcgctga ttaatcctat tgaaaaaaaa gaaagagccg ataataatga atttactatt
     8641 tccaaagaaa tctttaattc acaagatagt aatgaaatga tacatccaag ttaataaaaa
     8701 aattttatta tataaaggag gaagtatgga agtcaataat gtaaaattag agcatgtctg
     8761 tggaaatgcg agtcaatttc cggacacgaa tttaaatgag attgctttcg ctggcaagtc
     8821 taatgtaggg aagtcctcct tgattaatgc attagtgaat cgtaaatcct tagcaagaac
     8881 gagtgcccag ccaggtaaaa cccaaacgat taatttttat aatgtggaag gaaagttata
     8941 ttttgttgac ttaccaggtt atggctatgc aaaggtttct gcacaagaaa gagagcgttg
     9001 gggtaaattt attgaaagct atttagtgaa tcgaaaacaa ttaaaacaag tggttttatt
     9061 aatagatatt cggcacaaac cgggagaaaa tgacaaattg atgtacgaat ggcttcgcta
     9121 ttatcaaagt aacatattag ttgtagcaac caaagcggat aagataaaaa gaagtcagta
     9181 ccaaaaacat ttatcgataa ttcgcaaaga gctgggtatg gacagtagcg atatcttaat
     9241 tcctttttca tcggtcacaa aacaaggtaa gaaagagata tgggatctat ttgagcagtg
     9301 gcttttaatt gaggaataaa aataattatt aacagcaaac tttcgttaaa aaagcacgta
     9361 tataagtata taaattctac agtaacttat ggagcagttt attaaaaagg agggattcca
     9421 tgaagaaaaa gttatgtttg tccaatagag ataaaaaatt tgcgggggta tgtggcggta
     9481 tagctgagtt ttttgatatt gatgcaactt tagtgcgaat tttttgggct ttactcactt
     9541 tgatgtcaag tataattcca ggaataatcc tttatcttat ttgttgggcg attatgccta
     9601 tgcctgatga ctataaagat atgagataat agaagataat gaaagattag aaaagattat
     9661 gtaagaaaac aaataataaa agaaaaaaat gtccgatact tcggactttt tttttaaaaa
     9721 aactattgaa tagtaagtaa aatatagtta gaatatgatt atggacaaat atttagtaaa
     9781 ggggtaatat tatgaaaaaa acgaaagcta tcctaggttt accaattatt agcatatcaa
     9841 cagctgaaaa tgtggggaaa gtcaacaatt taattgttaa tggggcggat ggtactgttg
     9901 actacatttt agtagaaaca ggggataaag ccattaattc attagtaatt tcaacaagag
     9961 atattattgg tgtgggagag tatgctacaa cgattgataa tgaaggtgta atgattgata
    10021 ttagtagtgt accggatgcg atcgatttac tccaaaagaa tgttcaaata aaaaatacca
    10081 gcatattaac caaaaatggt aatttaattg gaaagagcgg agactttttc gtagatgaag
    10141 attttaatat tgctgcattg gaatttattg aagaaggaaa ttcagatgaa gtaaaattaa
    10201 ttcttagaaa agacgtaatt acttttggta aaaatttaat tgtggttgaa gaagatattg
    10261 aagacagaat attagattca gaagatgaat tattttcaga tgaaaagtca agttttttca
    10321 tagatccgat gaagaaggca ttgtagaatc tgctggtgtt cttgaatatt cactagatgt
    10381 cgatgaaggg caggaagaat ttattgaaaa agagccggac atgatgacaa tgtcttttga
    10441 ggatgaagat tcttttgatg ataatttaat aaaagatgaa agtttagaag aggtaaagca
    10501 agcggatata gagtctgtta aaacagcaat agcagaagcc attgctgcag cagaagcaga
    10561 gataaaagtt gaggaagaag tcctcgagga aaaaacggat gttgatattg cagtagaaga
    10621 agccgaactg gcagtagaag aggctgtagt agcagcagaa tctgtagcag gtttaataga
    10681 gccggtagag gatgaagttg aggaagacat taaagaagag aatacagaag agaatacaga
    10741 agaattcgtt gaagaaggat cagcaaaaga aacagaatca gaagaggctg ctgaacagac
    10801 agaagaacaa gcagaaattg aagaagagat tgttgcggca aatattatgt ccgaaccata
    10861 taacgaagaa gagacagaaa aattatcaga aaaagaattt ttctatattg atatggatga
    10921 ggatatgatg gatgaagatc aaatcgagac cattaatctt gatgaaaata ttttagattt
    10981 tgaagatgaa gattcgcaac tcgaattcga ttttaataat aacgacttgg attttaatta
    11041 tgtttcggat gatttgccca atgaagtatt ttatagcttt ggaaaagaga aagataaaga
    11101 taaagaagat gaagaagatg gctttatgtc ccgcattatg aaaaagatgc aagaagcagg
    11161 taatcctatt gatttagaga ggaataaaca attagatggt gtatttgaac aaaaacaaat
    11221 agaattctta actggtaaaa caatcaccaa agatattgtc accgatagtg gcaaagtaat
    11281 agctaaagct ggagagttaa ttagtgcaga aacaataaca aatgctaaga aaaatggtaa
    11341 actgatcgat gtgattatga attgtaagta aacatgttta cttttttttt agagaaaatt
    11401 actcctcgcg gcagaaattt gtaaaaaact attgaatatt gttgtaatat agctaaaata
    11461 gaattataga gataattaat cggtaaagga gtaaaactat gaaaaaatca agtacgctta
    11521 tcggattaac tgtcgtcagc ttagctactg ctgagaaagt cggtaacgtg agcagttact
    11581 tagttaatgc cgaaaaaggt agagttgatt atttagtgat tgaaaccgac catgaaatga
    11641 tgaattcgtt tgttatttcc acaaaagatg tccttcgtgc aggtaataat gcagttatgg
    11701 tagaaaatga agatgtaatg ctggataata gaagcgtagc tgacgccata aagttatttc
    11761 aaaaaaatat tcaaattaag gacacaaata ttctcacgga taaaggaaat tatcttggaa
    11821 ggacaggcga tttttatatt cacgaagaaa ggtttgacat taccggctta gagttaattc
    11881 cgagcgatgg tggagcaagc aaaatcattc ctagtaaata tgtaattata tatggaaaaa
    11941 ataccatcat tgtaaaagat gaagcagtac ataatttgtt aaactccgaa gatgaatttg
    12001 aggatgaata tcaggaagta gagcaggtat ctttttttat gggtccgatg aaaaagacag
    12061 catagatgct aaagaacctg aaatggataa tttagacaat gagctgtctt ttgaggatag
    12121 ggaaacacaa atcattttaa atgatgtcat tattgatgaa gatgaagagt taactaatca
    12181 tttaagcttt aactctccaa taacagatga atttgaacaa aatgaaaatg aattaagtga
    12241 ggatttttct tctttagaag agcaggacag caatttgact tttgattcta ctgtattgga
    12301 agatgaaaac atcgatctga atcaaaatga aattagttta gaagaagaag ccattacttt
    12361 agaccaggaa gttcaaagtg aattacattt tgaagaagag acagacacta tggatgagcc
    12421 tctgttagaa gatgacgaaa cgaaaatgac tttagaagaa atgatacaag aaactttaca
    12481 gagagagggt ttaattgctc ctggcgaaaa gtttagttta gacgatgtag aaccagttga
    12541 tattgattat gatttactgc tccagcagca gaacagtttt aattttggag gggacaatca
    12601 gtctgaatct gatttattgg atgatgatga tgatgatgac gacagtatgg atttaggcaa
    12661 tgattttgct gatgattcag atgatgatga cgaagaagtt aaagcaagta atttattatt
    12721 tgcagatgaa gaaaaatcag caaataataa attaagtgat agtttacttg attttgacag
    12781 taatgatttt gagcttagtt ttgatggaga aaaaagtctg gaagacaccg aagatgctga
    12841 taacgcttca tatagtttac tgatgaatga tgaaaatgag gaagacgata ttaatgatga
    12901 ttatgctagt agtttcggaa gtttctttga tgacattggt gtagaaaaga attccgaaca
    12961 agaattaatt gataaggtct taaaagatat tcaggcagca gattctggtg ctggatatga
    13021 tgaactgggt atggaatttt caaaaaagga tagtgttttc gaaaagaaac aaacaaaata
    13081 tttaacgggt aaaagagtga caaaagacat ccgtaatgaa catggggaaa tcattgctaa
    13141 ggctggggat gttattgacg aaaaaattat aaaaaacgct aaacggcaag gaaaattaat
    13201 tgagttgatt atgaattcta attaattaga agaagattta gaagaacgca tatattgcgt
    13261 tctttttttt gtaacacaaa cttaatattg tattaatatg gtcataatag aaataggata
    13321 tatttaggat attggttata aaaatggaat caattataag aaagaggttt gaatctttct
    13381 tatcgtcggg aggggggtta aaatgaaatt tgaacgccaa tggataccta attatacgga
    13441 attagaagat gaggaattat taaaacagat tgaattgtta agaaaagagc ttgaagatac
    13501 aaagaattgg ttagaagaat ctgctaatga aaaaggacca atggcatata tgaataaaag
    13561 gatggcaaaa cttgcacatt ctcttgcccg cgaaaaatat agattataca cagaagaagc
    13621 acaaaaaaga ggcattcttg attaaggatt cattgctggc ctgttggatt agtcagagaa
    13681 gccaggatag attgatataa agcctgggca ttattttccc aattggaaca aatgtttttg
    13741 gcttgctctt ttgaagctgt attaatttct aaatggatca atgatctttc atcttcttta
    13801 atagtacatt ttacaatata ttcattactg gcatttggaa agtattcagc ggatacttcc
    13861 agttcattct tcagacgacg gcggtttttt tgaatgtaac catctatttc tgacttaata
    13921 cttgcaggaa ttcggttgct gaaatattcc agggtattga ttcctttgtc tgcaatggta
    13981 tatcttgttg tatgattatc ttgttctttt attaccagat tgctgaccga taattctgtt
    14041 aagtattgct gtaaggaaaa ataatcaata tatccttttt ccataataaa atccgtaatt
    14101 tgggcatggg atatagggat ttggatcttg tgcagaagat acaataaaat taacttattt
    14161 gtagttaatt ctcttgttga atcaaacata ttttatcctc ccactatttt ctatattaaa
    14221 tatgatttct gtaaaacttt ccttgccagg tatttctttc tttgagtctt ttatgaatgc
    14281 cgtttaagac ggctttttta tttttgctcc acaagggtgc tataagtaaa tctttagaac
    14341 cgtctcctgt taagcggtga atgaccatat caggaggaag cagttccaga caatcgatga
    14401 taaggtcgat gtattcatct ttttccaaga ctttaaaagg atttttcaga tacatttggt
    14461 acaagggagt attttttaaa atatgaagaa gatgaagttt aactccttga actggctgca
    14521 tggaaatata ctttacagaa gaaagcatat cttctttgtt ttctccagga agtcctaaaa
    14581 ttaaatgaac aacactttca atttttcttt catttaaatt atatacggct tcattaaaac
    14641 atgaaagaga atagcctcta tgaataagtg aagcagtttt ttcatgaatg gtttggagtc
    14701 ctaattctac ccacaaataa gtctttttat taatttcatc caataagtca aggacatcat
    14761 gccccagaca gtccggacgg gtggcaatag ccaagccgac tacaccaggg aaagataaag
    14821 cttcttcgta tttttttctc aggatatcaa caggagcata tgtatttgtg aaggcttgaa
    14881 aatatgctat atattttcca gcaggccatt ttttttctaa taactcttta cctttcataa
    14941 attgctcaaa aagagataaa tctgcagcag ggatgaagtc tccggaaccc cggccactgc
    15001 aaaaaataca accattatcg gatatgcttc catctctgtt gggacatgta aatccgccat
    15061 ctaaagatat tttaaaaact ttttcattaa atttttttcg aagatgatag ttcaaggaat
    15121 gataggcaat gccatcccaa ttcataatta agctccattc aaaagatatt atctattatg
    15181 gtaaacctat ctaataaaaa aatcaatatt aaaaagtcca tattatttac gagagttaaa
    15241 atttcaactt cctgtaaata aatttttttc aaaaaggaaa caataattta taagacttag
    15301 ataaacatcg aggagagtct tatggatatt tctgtgatta taccagcgta taatgaagaa
    15361 gggagaatag gaaaagtcct gtcttcatta attgatcttg aatgtattaa tgaaataatt
    15421 gttgttaatg atggttctga tgacgggacc tcagaagaag ccaaaaaata tcccgttaag
    15481 gtcattgata ttgcccaaaa tatcggtaaa gccggagcga tgaagaaagg tttaattggc
    15541 agcaaaggcg aatggatatt atttttagat gcagatttga tgggtttatc cagagagcat
    15601 atacaagcaa tgattgattg tgctaagaaa caagatgcag atatggttat cggattattt
    15661 actaaaggaa atttaataac agatatttca cataaaattg ccccgtttct gtcggggcaa
    15721 agattaataa aaagagctat aatagaaaat attgattttg atgagacaat agcatatggg
    15781 attgaaatat atctaacaaa ttatttcagg aaaaaaggtt attatgttaa aaaggtattt
    15841 atgccaaatt tatatcatat tcccaaagag ataaaaagag gttttataaa aggtaagaaa
    15901 gaaaaattaa aaatgtattg cgatataata tttactgtga tccaacttta tttaataccc
    15961 aaatggctga aaaagttgta taaatgcaat atataatttt taaatcaggg tatgataata
    16021 ctccttactt tgtcttaata tttcacatat ttctttaaga tgcagccatt cttcttctga
    16081 atcaatcagt cttatcatat ttacaaaatc gtttcttact ttattactaa attcttcttt
    16141 caaccattcg atttcttcat ttgtttcaac ctgattcatt tttataagaa attctcgtgc
    16201 cctttccctt gaagtgaaag gaataaaggg ttctaattta tctaaagcag cttccaaagc
    16261 ttcatattgt ctttgaaaag gattatcttc aacttcggca atgtaagtaa ataaggattc
    16321 cagattaaga aatgtaacat tctctaattc tgagtaatca aagttctgaa ttaaatctct
    16381 taagttatcc aattcaaaaa aaagttcttc ccgacttaag atatttgaca tattaaccct
    16441 tctttcatag gaatatgttg atacaatatt aatatttgca gaagccattc aaaaaacaca
    16501 attaaaattt gttgctttta gcggataaag ctataattgc ttttaacagg aaatattaat
    16561 ttttttaaca tgaaaattaa tttttttagc aggaatatta agtatttatt agatattatt
    16621 aaatttcatt aatatatatt gcgcttcatt tttcgtctga taaaattaaa aatgaagttt
    16681 ttaataaagc ttattaaaag ccaaaataaa aaattagggt gaacagattt tgcaaaagaa
    16741 atttttgaat aaaaaaatga tatttactgt cttttttttg gtagttataa gtttttcact
    16801 gggtttatgc tttcagaatg catctgcttc atcgttttta atttttttaa acgataagca
    16861 agattctatt aaatgctatc ctaagggggg ttatatcaaa tacaaagggg aaaaatatta
    16921 tcttgtaact gaaacagata aagtgattat cgatatgaca gcagcggata ttgacaataa
    16981 tatgaaagaa gatattttaa tcattgaagg caaaaaggac tctgaatacg gggataacct
    17041 gattatatat gagactgaat ttttgccagg caaaataaaa ttaacagaga aatatcgtaa
    17101 taacttaact gccattaatc cctggaccat taaaatatgt aatattgaca atgacagtga
    17161 accagagatt tttattggta catataaaga gaccgtttat tatccgggat tagaaaacag
    17221 accattcttt tttaattata gggataataa acttgtgaaa aagtggactg gttcgaaact
    17281 gagagcgcct tttaaagata tttgttttgg agatttaaat gataatggga gtgatgaatt
    17341 aatcgttata gaagagcttg aagaagataa aaatattata tccgtatatt attggtttgg
    17401 atttggtttt attttgtatg ctgaaagcag cgtatataaa aatattgagt ttgtggaaac
    17461 tgaaataatt gatgggaagc ttcttattaa aacggatcag ggttatttgg aattatcggc
    17521 agataaaagt gaaagcggtg tttatttatt aaaggagagg tgaatttgaa tgtcaaagaa
    17581 aatcatagct gtttttttat taatgactat tttattaacc gcttgcagca gttctcgtac
    17641 aggaatgtca gcgggtgcaa attcagggaa gagtatttct gggaaatatc tgttaaagca
    17701 tgcagatact tcggcttata acaatgaaac agttcccttc acagagactt cgtataagcc
    17761 ttctattccc caatatagcc taaatcctga tctgagcaat gttgaaaatg cggaacaatt
    17821 tgaaggcctg acacaagagc aaattaatat gctggtcaat aacggattta tagttttaaa
    17881 tcctaatcgt aataaggctc actactattt aaagatgtat gatatttatg aagaaaatga
    17941 atataaaaat gtacccaact ttattacggt tgacgttgct cttcatattt atcataagtt
    18001 ttatgatgaa actttaaaaa gtatcgaaaa agagcatcta tatacagctt tgcaggaact
    18061 gactgaaaat atgcttaata aaagtatagc tttatataaa gaagaaacaa gcaaagacac
    18121 aaaagaactt ttagaaaata ttatggtata tttttctgtc gcaaacaagc ttataaataa
    18181 tagttacgga gatataccag aggagttgct tcctgttgca gaagaagaaa taagtcttat
    18241 tgatgcggct aaaggatata taaagtctcc tttactggat tatgatttga actatgaaga
    18301 atttactgtc agaggacatt atacaggaga tgaaatatta gagccttatt ttaaaacgat
    18361 gatttggtat ggtataggtg cttatccgct tgttgatgaa aatggaaaaa tcgattatga
    18421 atccgcacaa aaagctttag ccataactta tattttatta cttgataacg atggtagtaa
    18481 tgacattgct ctttgggata aaatatattc tccaaccaat ttatttatcg gtcagtcgga
    18541 tgatatagga atccttgaaa tcaaagaatt attggttaat gtttatggcg atgatgttaa
    18601 tcttgaaaag attacagata aaaaatacaa aaagaaatta gcagaagaaa tcgaaaaatt
    18661 gccatcaccg caaatagaat ataaattagt aacgggtaaa gtagacacac cttcagacaa
    18721 gcaattcaga ttcatgggtc aaagatatac tttagatgct aatattatgc aagagctgat
    18781 gttccccatt ataagaccag ttccaacagg cttagatgtt gcaggtgctt ttggaagtga
    18841 aagggcagag gctttagcga aacaatactg tctgaatgat ttagatgaaa aagaatacag
    18901 cagtaagtta actgagttaa agaataaggt taaggcactt aaattagaag attggcaaaa
    18961 gaatctttat aatggctggt tatgggtttt agaatccctt tggacaaaag attataaaaa
    19021 agaaggttat cctgtcttta tgcaaaatca ggcatgggag gataaaacga tttccagtgc
    19081 tttaggaagt tatgcagaat taaagcatga taccgttctt tattcaaagt caccagttgc
    19141 tgaaatgggc ggcgggggag aactccagga aaagtatcct cattatgtag aaccagctgt
    19201 ggaagtatat gacagattgt tatggttagt cagatattcg aaggttaatc tggaaaagag
    19261 gggcttacta tcagaaaact cagcagatgt tttgaaaaat atagaagacc tctatgaatt
    19321 attcagggtt tgttctgtta aagaactgga gaataaaccg ctttctgaag aggagtatga
    19381 agctttgaaa tatattggtg gcagactgga atggatagat gatcagttat ccaatcaata
    19441 taaccaacca ttaagtcctg cagttgtttc ggatgttgct ggtttagccg atgttggtat
    19501 gtttgttgaa ataggtacag gtttaccaaa tgacattatc gtagctattt atgataatgg
    19561 aaagatatat ttagcaagag gagctgtata cagttattat gagttcttaa gcgataaacc
    19621 attaacagac gaagaatggc atgatattat tggtgtcaaa aaggttgtgg atggcgaatg
    19681 ggaatttgaa aaggtagatc cggattcaat aggaaatgag gcagaattca gacctccatg
    19741 ggtaaattct tttaaatctt ttgaagagaa taaagttaat attccggata tagaatatat
    19801 tattccggat tcagaggaat aagaaaattt taaggggctg tgcacgtatt aataaaagtg
    19861 acagcccctt attgattgat ttgttctttc caaatataaa atattttttt gtactcactg
    19921 ctttttgtag ctaataagtt gataaattct tcttgttttt ctattgatat gggagtaacg
    19981 gctttttcaa acaatttatt cattttatca tactttttaa gaaaagaatc aacgctggca
    20041 tcaatcattt cctgggaaga aatcttagta aaatttacat gataacctgc aaccagtccc
    20101 aaatattcaa taaagagtct ttgggttctt ccatttcctt ctctgaaagg atggatggca
    20161 tttaactctc caagataata ggctaatcgc tcaatgacct gcttttgatt taaaccaata
    20221 agatagtgct ctctatgaag ttgggcaaac aattctgagg ccacatcatt gatatattta
    20281 aaatcacaaa aaggatttcc ttttgaaata ttaacagtcc gtgtcatacc tgcccatgga
    20341 taaatgtcta taaaaatgta tttgtgaata gacaacaaat ggtcaaaatc taaatttcct
    20401 tttaaaggat tcatttttaa atctaaaatt ttaatggatg taatttggcg ttctgctttt
    20461 tcaagctcct ctttattttt aatgtttaat ttattcctta aaacaaaaga attgggataa
    20521 caataattga tatcccaagc aaactcataa taagaattta acataataac caccgttgta
    20581 ccttttattt ttttgtatat tttttaacca gacacttaac taaatgagaa gaagacactt
    20641 gtccataggc acatttttta agcatatcaa tttcttgttt atctaaataa aagccttcta
    20701 tggccattgt gctttttact tcttttacca gctgggaagg attaactaca taaaatgaac
    20761 tcttcatgat gacctccatt ctttataaga gccctttcaa acgcaaaaaa taaagctttt
    20821 cttgatataa ttataccata tttattgcaa aaatacacct tttatttctt tacatttttg
    20881 tattttagca ttaaaaaatt ttttaaacta ctaaaaattt taattttaaa aacgtatcca
    20941 tttcatttca aataactatt aaaattttaa aaattttata gaaatatatc aaaaaatatg
    21001 ataaatttaa atatatattc tgaattaata taaaaaatag atttgtcata ttaacaataa
    21061 aactattttt atta
//
LOCUS       DAOSUD010000037        20789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1670_length_20789_cov_22.510080, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000037 DAOSUD010000000
VERSION     DAOSUD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_20789_cov_22.510080"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1091)
                     /locus_tag="PLG49_06195"
     CDS             complement(105..1091)
                     /locus_tag="PLG49_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ16950.1"
                     /translation="MKVEISREKVVSKKNKKTSRLIFEIRKNRFAYALVLPAFIAMLI
                     IHFIPMVQGIYMSFLKLNQFTLNQFLKAPFVGLQNYYTVLFDKSSTLRSGFMDALRNT
                     IIYALSVNILVLVIGMISALLVNRKFKFRGLARTLLLTPWVVPSYVVGMLWGFMWQQD
                     TGIINRILVDWLHILPDKPFWLSGPNTLWAIIIPTVWRSTPFIMIMLLAGLQNIPEDY
                     YEAAEIDGASSVQKFRHITLPLLRPIIAVNLLHGMITHIYSFNIVAMMFGHGAGFPGK
                     WGDLLMTNINRNSFGIWQMGTGAAATIVVMIIVLSIVGIWYRVFREDLVVEE"
     gene            complement(1160..2542)
                     /locus_tag="PLG49_06200"
     CDS             complement(1160..2542)
                     /locus_tag="PLG49_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOQ16951.1"
                     /translation="MKKRVISLLLACLMIFAVAGCSKSQSGKDNDSTPTVSKETEEKE
                     SKEPVTLNVWIMPNSGEPEKDFMDVIQPFLEENPNITVVPTVIDWGSAWTKVTTAATS
                     GEAPDVTQLGTTWVAAVASMDALADLTPYYDEFGGAEAFLPATLPTTVIEGSDQRFAV
                     PWFVDSRALYYRKDACEIAGVNPETDFATWDSFKEALKKLNGVEVDGKKMVALGMPGK
                     NDWNVVHNFSWWIWGAGGDFVSDDLKTSTINSPEAFEGVKFYTELAIEGLMSMPSLEK
                     NSAEIEALFNAGEFATIFSGGYFAKTIKDNAVKDPENSIVDKVGVAMIPEGPKGRHVF
                     FGGSTLAVYKTTKHMDEAVKLIQFLTSKEAQIEYSKRHGNLPATKEAYEDPYIDQDPM
                     LSVFKEQAKYGRAYPSIPGWAPSESLLQKGLSNVWDNVMGVNGPYDPERTKIELDAAA
                     ADATAVYQAN"
     gene            complement(2780..5437)
                     /locus_tag="PLG49_06205"
     CDS             complement(2780..5437)
                     /locus_tag="PLG49_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HOQ16952.1"
                     /translation="MERKLVMGILANVDAGKTTLSESMLYISGKIGKMGRVDKQDTYL
                     DTDEIEKSRGITIFSKQAVFEMASTQITLLDTPGHVDFSMEMERTLQVLDYAILLISG
                     PEGVKSHTLTLWRLLKIYNIPTFIFVNKMDQETADREELIKALKSQLSDRCISFEKDY
                     GEGFFEELAVCSEELLEAYLSTGHIEKDLIKEKIKARKVFPCFFGSALRLEGVEYFLE
                     KMIDYSLIPFYPEDFAAKVFKISRDEQGNRLTHMKITGGRLKVRDLIKGSDWEEKITQ
                     IRIYSGKNYEAIGEVAAGTVCAVTGLSKTRPGEGLGLEKDFSKAVLEPLLSYRINLPH
                     GYSPREVLPKLREIEEENPELHITWDETLEEIQAQVMGEVQIEVLQSIIKKRFNLDIS
                     FDDGQIVYKETIANTVEGVGHFEPLGHYAEVHLLLEPGEPGSGLQFGSNCSEDLLARN
                     WQRLILTHLEEKDHKGVLTGSSITDMKITLVAGRAHNKHTEGGDFRQATYRAVRQGLK
                     EAQSILLEPYYSFQIELPTNMVGRAMTDIEKMHGTCEIYKTGEEMTILKGTAPVANMR
                     NYQKELTAYTKGFGKLFCTFKGYEPCHNAEEVIERINYNSEADPKNPTGSIFCSHGAG
                     FYVSWDKVKDYMHVESYLKAKKEIEEKIPENKIPSATEVISLEEIDEILGKTVSNQGK
                     GNKWKKTKSSVERYYESLSSGNRPTKKEKAKPTYLLVDGYNVIHTWPELKKLAQENMD
                     SARIKLLDILSNYKAIKKYEIIVVFDAYKVNGYREKIMDYHNIHVVFTKEAQTADEYI
                     EKFSYEHNKDYNIIVATSDYLQQIIVRGSGSTLLSARELKEEVTSTRKSHIDRYIDLQ
                     DRRTASLEESISKDTKSKIKAIKKPLNNQ"
     gene            complement(5697..5876)
                     /locus_tag="PLG49_06210"
     CDS             complement(5697..5876)
                     /locus_tag="PLG49_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOQ16953.1"
                     /translation="MDNYEVVLEYFRKADKPVNAGTVAQETGVDRKEVDKIMKKLKEE
                     GKIVSPKRCYWEINK"
     gene            complement(6609..6986)
                     /locus_tag="PLG49_06215"
     CDS             complement(6609..6986)
                     /locus_tag="PLG49_06215"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOQ16954.1"
                     /translation="MQKYNVFVYGTLMKGFSNYKHYLEGRSNVKFLGMAKVEGYSMYH
                     VSYFPGIVPDKGESILGEVYQVDEKLLRQLDILEAEGHMYIRQKESAYLDTGEKIDVY
                     LYVWNLPVKENCKKVEMMPWRPL"
     gene            complement(7117..8175)
                     /locus_tag="PLG49_06220"
     CDS             complement(7117..8175)
                     /locus_tag="PLG49_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOQ16955.1"
                     /translation="MTFEEAVEKYLATLKNEEEKDSCQAFLMDCYMPIVKKEAEKHKL
                     WPLELKKIRENETEWIILADSIDTVLDVDSAQLNQSKNDMEENEYTKRKAYQYIKAFY
                     NFIEKKCNSRLKIQNLFYQGRILENKIERQLDIIKKLQETPMTKDELADYYCVTRKSI
                     DRDIDELQIDGLQLLGQKVKLKVEYRLSQKGGANTRSQELYYSSTAHPIFLPLNMTQV
                     YAMTAGLKKLARGSEYESILNHIADWVYSQLTEYAKDIIQNSIDRQGERISFKEVKPE
                     FREERKMFSNNIREELVFLEKSQKKAVIHYKNPDTGLKEVAIGYMEILDPNKIGVYQN
                     NEYILIKIDWIEKLDYTY"
     gene            complement(8194..9030)
                     /locus_tag="PLG49_06225"
     CDS             complement(8194..9030)
                     /locus_tag="PLG49_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOQ16956.1"
                     /translation="MAVVNYFAYGSCMNEKDFIRDLKKAHLEKSYKILGVGKLEGFRL
                     AFTRYAFTRGGGVLDMIPSLGDYVLGVVYQVPEKALIKVIDRREGVSGGAYYRDLIEI
                     ELNGEKIKAYTYFVKNKLEHEIEPSESYKEIVYEAMKNMNFPEEYINKYLLEHIASLK
                     RNDNDKVSYEAEVYDSSSYLDSMGTYVIGMPKSLRNLLSVDIGDWIYVRYEEKELKLK
                     VYKTDSRLLGNGQAPADNLQFHVCIPRQVRDELGLKELDAFHRKNYPVFKKKFSCVTI
                     YK"
     gene            complement(9043..9726)
                     /locus_tag="PLG49_06230"
     CDS             complement(9043..9726)
                     /locus_tag="PLG49_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16957.1"
                     /translation="MYIHLNEVPWNAFFIMAEEEYGFKILNSEEIDSMTQYIYVRYYS
                     GVEKAYCLRFEYQEDWGQWQLALYAENAIRCLSALDLYAIQGVIAPQIRDYRAYLGIN
                     PNKYIHWDLIIDRLFCGPVYEVLKVDDLTRYVRLESQQGTKEYLVGFVIIPELDYRME
                     VQVYDARDGVYLTSLDSIIVDTKVFVDYPVAEEYLNPTKQPPDYGVFIKREPYGRGRI
                     NRGLNKEYR"
     gene            complement(9819..11138)
                     /locus_tag="PLG49_06235"
     CDS             complement(9819..11138)
                     /locus_tag="PLG49_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16958.1"
                     /translation="MKKKILLIQPTPYDQKGNLIKKSRLYFVGLALPLLAALTPKEYE
                     LELCYETIEEVPFDTDADLIGISSMGHAVIRSIDIAKKFKELGKTVVLGGYMVSLMPE
                     EAQKYCDALVIGDAEESWPELLKDYEEGKLKKLYQRKLKALKTPLPKYELLLNKKIGN
                     FLPVQAGRGCPKACSFCSVYCLYRGQYLKREIPEVIRDIKRVKELGFKQFLLLDDNIF
                     SNREYALNLCAEIKKLKMKWMTQCSIDIASDEELLKAIAESGCYVLSFGLESISKESL
                     RSMNKAWAKPENYARQIKIIRQHGIDISTEMVVGADGDTLESIKNTAKFISDNHIAVP
                     RFYILTPIPGTKYFEEMQKAKRIYNTDIYSYNGCEAVHIPKNMTPEELTKAYWDLYNE
                     VYSIKNIVKRTLLTKTFLSRPANALFYFGVNLYYRSQIKNGIVPNII"
     gene            complement(11135..11782)
                     /locus_tag="PLG49_06240"
     CDS             complement(11135..11782)
                     /locus_tag="PLG49_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOQ16959.1"
                     /translation="MTWEKEDRNCGLCGERTCKDFRLSIENGKRRLNDCPFYNNNNVY
                     KETIVEEDHLDIHNNVYDFVLKAIADEPSARKIIHPFRVDLIEKLNIQAGDIVIARPV
                     GAGCPVTHVLKVYEVDELAGLLYTWVVGPKYSRGKEVKDIKAYSMVGFEGIAEQIIYP
                     PEVGRTSAFMPGFCMLRLTHYGLVNKVYNTKEGLLVRIEDIHIAHYEHIQRRSQQ"
     gene            complement(11785..12495)
                     /locus_tag="PLG49_06245"
     CDS             complement(11785..12495)
                     /locus_tag="PLG49_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOQ16960.1"
                     /translation="MKLLLVGGPPSTGKTTVIQRIIEKRQGKYKMAYLKMDVVYAHEE
                     ELIKEKYNIHARTVYAGDLCPDHAVVMILTDAIRWAEDLKADLLIIESAGLCLRCSPY
                     LNQGLGLVVLSMLSGIHSVDKMKQLICFADVAVLTMNDLVCQAEREVYIKKLKNSFND
                     LKVIETNALQGIAIEYLCNLIDESEEIHLGNLELKGNPPLGTCTICIGSKKIGWKNHY
                     GVVRQIDSNAADYMYRGD"
     gene            complement(12516..13265)
                     /locus_tag="PLG49_06250"
     CDS             complement(12516..13265)
                     /locus_tag="PLG49_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ16961.1"
                     /translation="MEREISIYAGFDKSGKKEPYDKITFKLGEVISIVGHTGSGKTLL
                     ISDIEQLSQRDSASKRMITIDDKVPGYEERFNPEKKKIAMITQNTKCVSDLLVWEFLK
                     IHADSRKIKNKDLIGEVIDLANKFTGESISEKAKVTELSGGQTRALLFADAILIGASP
                     IILVDEIENAGINKTEILKHVKKENKLVIFATHDPSIVLQTEKRILMKNGAIEKITEY
                     SKKDKEVLALVLKADKTIKEIQDKLRNGIDF"
     gene            complement(13307..14689)
                     /locus_tag="PLG49_06255"
     CDS             complement(13307..14689)
                     /locus_tag="PLG49_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16962.1"
                     /translation="MKVLLIRPHLNKTVTTIKKLMFGEPLGLECVSAILKEQGHEVLL
                     VDFLAESKRNLRKYIKSFKPDVVGFTSQCSDISNVLYLAEKTKKINHKITVIVGGIQV
                     TITPEAYFSRYVDYVVKTTTRENMKALMEQIEKDTDEEIMGVFSKKRQFQSTISSCEN
                     EYVKPDRDAAKKYRHKYKYAGYQPYATIQTSYGCRNHCSFCIRRKLEGPGLKERPIED
                     VVAEIEELKEDYVMICDSDFLINEKRLIRFLDLLEERKIKKTYICYGSVNSILEKEYL
                     FKRLSENGVKAVIVGLESFTDAFLKEYNKSANLDDNYKAVRILKKNGIATWGTFILHP
                     DFSKEDFKTFKKYLKFLKPELISFTPLVPHFLTPLYKQYKDRLIYPRDDYEKWSFGDV
                     IIYPSKMSLRRYYFEVLKLGIPANFNWCTIKYCFTTFPVKRNIGLMFGFDQILKVYIK
                     NIFNPKKLPE"
     gene            complement(14697..16061)
                     /locus_tag="PLG49_06260"
     CDS             complement(14697..16061)
                     /locus_tag="PLG49_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16963.1"
                     /translation="MKVLLIRPKPHKETIGLQNVMICEPLELEYLSSNIEAFGHQAVI
                     VDMILEKRKVTYFIKKYQPVLVGMTGYNTHINVIKTYAKQIKACNPEIKIVVGGVHAE
                     VNPEDFLSEDIDYIVRANGIRTFIEILNKLEKNEDVSVIEGIYHQKNEKAEKEKQFNY
                     LFPDREKVKKYRSKYYYMFHRNCSLIKSSFGCPYQCKFCFCRKVTDGHYFTRDLENII
                     EELKSIKEKEIYIVDDDFLVDRERILRFCDLLEKNKIEKRYLIYGRADFVASNEDVIK
                     RFKALGLRAVIVGLESCRSNELLDYNKKTQVETNERAVKILQKYDVECYGTLILGLDW
                     EKEDFMALAKWIKKLGLVFVNLQPLTPLKGTELYEQYKGNFIIKEDEYEKWDLAHLVV
                     KPSHISIRKYYYYTILLYYKITLNPKSVWYMIRKYGLHDTIKLSIGAFKINLQYFKKL
                     IGGK"
     gene            complement(16058..16684)
                     /locus_tag="PLG49_06265"
     CDS             complement(16058..16684)
                     /locus_tag="PLG49_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ16964.1"
                     /translation="MRYEVWEKLAHKYNRLWVQKYSLGPTRREILKIALPMLEENPNL
                     KILDIGCGTGQLIGEISSQYKELYYLGIDVAKNMIEIAKSNNKEKNIEFRVCPVENFT
                     CQEKYDLIICSHSFPYFPNKEETLKKIAALCNPHGQVIIVNSSTNNLKDLIINFFLKA
                     TTSKARYLSIKEMKKLFQAARLKVKKIKIIRERFYMPTIALFHLEVQP"
     gene            complement(16698..18038)
                     /locus_tag="PLG49_06270"
     CDS             complement(16698..18038)
                     /locus_tag="PLG49_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ16965.1"
                     /translation="MRVLLVKPEFKNVFTKLSLITTEPLELEYMAAICKRNHIDAQIC
                     DLSIKDKSLKYMLNHFKPDIVAMTASFVHIHSIKKYVACIKKFNPSIMTMVGGPHAEV
                     MPEDFQFEGIDIICYSGGFKPFEEIIKSNNKTDFKKIKGIYYYKDGDWHKNEKEIFDI
                     ESLPFPDRSHFYKNIKKYKYVTMKPCAIVKGSYSCPHKCNYCFSTLLNGGRYLCREPE
                     NLLEEIKSINCENIWIVDDTFYVDKEKLDRFIELAKKEKLQKKFSLYYRADFIAQNPE
                     YMKRLAEIGVTMCAVGLEVIDDEILNKYNKKTNVHIIQRALEVLKEAKISCIALLMID
                     IDADKSYFKKLYNFIRQHELYLATISVLTPMPGTQQFEKYKDRIITDDYRKWDFIHLI
                     IEPAKMSKLTFYKEFYKLYVKLALLILRNRILSFKYIKAAIGASFNYWLEVLKG"
     gene            complement(18042..19022)
                     /locus_tag="PLG49_06275"
     CDS             complement(18042..19022)
                     /locus_tag="PLG49_06275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16966.1"
                     /translation="MKKQFKHLKIYHSDDFNKIKLNIKLPYPFEKDLLRLLKKENKFE
                     QEYYYLEQGKDYAFFVLYKNKMNIFTFGKLKFNMKLKVIGYPCSLSNAGYVTNNEKMM
                     LDYIKSLKGAKLVLNVSNPTGSEGITVGQTLPTCVFYNRFHSVTEYLTALRSSYRRRI
                     NKAIKNCEDLEIKINDETIDVYDLYLNTYNKSDYKLEKLESNFFKVFDGTKIVFLKDK
                     KAIGFVLLKKVEDKLIFMFCGMDYRYNTADLYYYMLYKIIEYAIDRQCKIIDFGQTSE
                     ETKMKLGALLEKRYFYAHHSNPIINFLVNIGKDFLEYKYDFPQYRVFKEG"
     gene            complement(19006..20088)
                     /locus_tag="PLG49_06280"
     CDS             complement(19006..20088)
                     /locus_tag="PLG49_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16967.1"
                     /translation="MRVIEVKSKKGQKKFLDFRKKIYQSNPVFVDNHMFMLKMLFANK
                     TCFSNNKKIYVVNVEDDGKVLCQGLIIHAQELAEYIQLCFFESKANQEKAVELLVDKA
                     VEVGKRYHCKKLVVGLNGHLNYGLGLLNSHYDEKNSFSASANPKYYNDYFKKMNFEEV
                     HLKTYKIDRIDDRLKVYEGIIDRLNKKYRFEYFDKKNFDYYSKIYTDLNNQCFMNHRY
                     YYKRGYEEDKEMLKELFLFMKNDSLIFVFDGKEPVGFTLWYQDYNELVKKGDAFGTKH
                     YFKNKLFNKKIKKAKVMEIALLEDYRKIGLPLGLLNQIKLCLDRYKIKSLETSWVLEE
                     NYASNSVCKAICDEEYKRYVVYEKAI"
     misc_binding    20499..20747
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6299 a         4191 c         2856 g         7443 t
ORIGIN      
        1 tacaattggg aatatagtaa atataacaat tataaagagt aatatattgg cagctatatg
       61 ctgaattttt ttcttccgtt tcaagttttt catgtaatta ttcttcattc ttccaccacc
      121 aaatcttctc taaatactct ataccaaatt ccaacaatac ttaatacaat aatcattact
      181 acaatggttg cagcagcacc tgtacccatt tgccatatgc caaaagaatt acggttaata
      241 ttggtcatta ataaatcacc ccatttacct ggaaatcctg caccatgacc aaacatcatt
      301 gcaacaatat taaaagaata aatatgagta atcattccgt gaagcaaatt aacagcaata
      361 attggcctga gtaaaggtaa tgtgatatgt ctgaactttt gaacagatga ggcaccgtca
      421 atttctgctg cttcataata atcttccggg atattttgaa ggcctgctaa tagcataatc
      481 ataataaaag gggtactcct ccagaccgta ggaatgataa ttgcccataa agtattcggc
      541 ccactcagcc agaaaggttt atcaggcaat atatgtaacc aatccactaa aattctattg
      601 atgatccctg tatcctgctg ccacataaaa ccccataaca ttcctaccac ataagatggt
      661 acgacccacg gcgttaataa taaagtcctg gccagtcctc tgaatttaaa ctttctattg
      721 accagcaatg ccgatatcat accaatcact aatacaagta tatttactga taaggcataa
      781 ataatggtat ttcttaatgc atccataaaa ccagaccgca atgtgctgga cttatcaaat
      841 aatactgtat agtagttttg caatcctaca aaaggtgctt tcaaaaactg attcaaagta
      901 aattgattta attttagaaa agacatatag ataccttgta ccataggaat aaaatgaata
      961 attaacatgg caataaatgc aggtaaaact aatgcgtatg caaatctgtt ttttctgatt
     1021 tcaaatatta acctgcttgt tttcttgttt ttttttgata ccactttctc tctggatatc
     1081 tcaaccttca tacatatctc cctctttgat tattaaaaat cagtgcagag aatcttctct
     1141 gcactattat tcactttatt taattcgctt gatatactgc agtagcatct gccgctgctg
     1201 catctaattc tatctttgtt ctttcaggat cataaggtcc gtttactccc atcacattat
     1261 cccaaacatt acttaaacct ttttgaagta agctttcgga tggagcccat ccaggaatag
     1321 aaggatatgc tcttccatat ttcgcctgtt ccttaaatac ggacagcatt gggtcctgat
     1381 caatatatgg atcttcataa gcttcttttg ttgccggaag atttccatgt ctcttggaat
     1441 attcaatttg tgcttcttta gaagttaaaa actgaatcaa ttttactgct tcatccatat
     1501 gtttagtcgt tttataaaca gccagagtac ttcctccaaa gaagacatgt cttcctttgg
     1561 gtccttcagg aatcattgct acaccaactt tgtcaacgat ggaattttcc ggatctttta
     1621 ctgcattatc ttttatggtt tttgcaaaat aacctccaga aaaaatcgtt gcaaattctc
     1681 ctgcattgaa caaagcttct atctctgctg aattcttttc taagcttggc atagacatta
     1741 aaccttcaat agcaagttct gtatagaatt taacaccctc aaaggcttct ggtgaattaa
     1801 tcgtggaagt ttttaaatca tcacttacaa aatctcctcc tgctccccaa atccaccagg
     1861 aaaaattatg aactacattc cagtcgttct taccaggcat acctaaagct accattttct
     1921 ttccgtctac ttctaccccg tttaattttt tcagtgcttc cttaaaacta tcccaggtag
     1981 caaaatctgt ttcaggattt acccctgcta tttcacaagc atctttcctg taataaagtg
     2041 ctctggaatc aacaaaccat ggcactgcaa acctttgatc gcttccttca ataactgtgg
     2101 ttggcagtgt tgctggtaag aaagcttctg ctccgccaaa ctcatcataa taaggcgtta
     2161 aatccgccaa agcatccata gaagccacag ccgctaccca agttgtaccc aactgagtaa
     2221 catccggagc ttctccactt gtcgctgcag tcgtaacctt tgtccaggca gaaccccagt
     2281 caattactgt aggtactaca gtaatatttg gattttcttc taaaaacggt tgaataacat
     2341 ccatgaaatc cttttcaggt tcaccactgt tgggcataat ccaaacattc aatgtaactg
     2401 gctctttgct ttctttttct tccgtctctt ttgaaacagt tggcgtgcta tcattatcct
     2461 tccctgattg agattttgaa catcccgcca cagcaaaaat catcaaacaa gctaaaagca
     2521 aagatattac tctttttttc ataaatatcc ccctttatct attctgcatt tttagctgtt
     2581 cagcaatata ataaattatc tcatctgtaa aaaaaatatg gtaaaattac gattatattg
     2641 taataaattc acataattta tttttattat attttctccc aggtaaaatc gacgattgaa
     2701 cagaaaaata aaaaccgctt tttagctaat tagcttaaaa gcagttttta tgataaatat
     2761 ttcttaaatg aatctttatt tattgattat ttagtggttt tttaattgct ttgattttgg
     2821 attttgtatc ttttgatata gactcttcca gggaggcagt ccgcctgtct tgtaaatcta
     2881 tatatctatc aatatgactc ttacgggtac tagttacttc ttcttttaat tctctggcag
     2941 acagcagggt acttccactg ccccttacaa taatctgctg taaatagtcc gatgtcgcca
     3001 caataatatt gtaatcctta ttgtgctcat aagaaaattt ttctatatat tcatctgccg
     3061 tctgggcttc ttttgtaaag acaacatgaa tattatgata atccatgatt ttttccctgt
     3121 aaccattaac tttataagca tcaaaaacaa caataatttc atatttttta atggctttat
     3181 aattacttaa gatatctaaa agcttaattc tggcactgtc catattctct tgcgccagtt
     3241 ttttcaactc cggccaggta tgaataacat tgtaaccatc cacaagcaaa taggttggct
     3301 ttgctttttc tttttttgtt ggtctattcc cagaacttaa agattcataa tagcgctcta
     3361 cagaggactt tgtctttttc cacttgttcc cttttccctg attggatacg gtttttccta
     3421 agatttcatc tatttcttcc aaactaatga cttccgttgc agatggaatt ttattttccg
     3481 gtattttttc ttcaatttcc tttttagctt tcagatagct ctccacatgc atatagtcct
     3541 taaccttatc ccaactaaca taaaaacctg ccccatgaga acaaaagata gaacctgtgg
     3601 gatttttcgg atctgcttcg gaattgtaat taattctttc aataacttct tctgcattat
     3661 gacagggctc ataaccttta aaagtacaga ataatttacc aaagcctttt gtataagcag
     3721 tcagttcctt ttgataattt ctcatgttag caacgggcgc agttcctttt aagatcgtca
     3781 tttcctcacc cgttttatat atctcacaag tcccatgcat tttttcaata tccgtcatag
     3841 cccgtccgac catattggta ggcaattcta tctgaaaact atagtaaggc tcaagaagta
     3901 tagattgtgc ttcctttaaa ccctgacgca cagcacggta ggtggcctgt ctgaagtccc
     3961 cgccctctgt atgcttatta tgggcacgac cagccactaa agtaattttc atatctgtaa
     4021 tcgaagaacc tgtcagtacc cctttatggt ccttttcttc caaatgggtt aaaatcagcc
     4081 gctgccaatt ccttgcaaga agatcttcac tacaattact gccaaattgc aagccgcttc
     4141 ccggctctcc cggttcaagt aaaagatgca cttctgcata atgaccaagc ggctcaaaat
     4201 gcccaacccc ttcaacggta tttgcaatcg tttctttata aacaatctgt ccgtcatcaa
     4261 aagaaatatc aaggttaaaa cgcttcttta taatgctttg caaaacttca atctggactt
     4321 cccccatgac ctgtgcctgg atttcctcta aagtctcatc ccaggtaata tgaagttcag
     4381 gattttcttc ttctatttct cttagtttgg gcagaacttc tctgggagag tagccatgag
     4441 gcaaattaat cctgtatgat aataaaggtt ctaagactgc tttactgaaa tctttttcaa
     4501 gacccagacc ttcacctggt ctcgttttac tcaggcccgt tacggcacaa actgtcccgg
     4561 cagctacttc tcctatagct tcgtaattct taccggaata aatacggatt tgagtaattt
     4621 tttcttccca gtcactgcct ttaattaaat cccggacttt taacctgccc cctgtaattt
     4681 tcatgtgggt taaacggtta ccttgttcat ccctgcttat tttaaatact ttagctgcaa
     4741 agtcttccgg ataaaaaggt ataagggaat agtcaatcat tttctccaaa aaatattcaa
     4801 caccttctaa cctcagggca gacccaaaaa aacagggaaa gactttacgg gcttttattt
     4861 tttccttaat caaatccttt tcaatatgac ctgtactaag ataagcttct aacagttcct
     4921 cactgcagac agccagttcc tcaaaaaagc cctctccata gtctttttca aaagatatgc
     4981 atctgtcact taactggctt tttaaagctt ttattaattc ttctctgtct gctgtttcct
     5041 gatccatttt gttcacaaaa ataaaagtgg ggatattata aattttcaaa aggcgccaaa
     5101 gggttagggt atggcttttt accccttccg gaccactgat taacaagata gcataatcta
     5161 aaacctgtag ggtccgctcc atttccattg aaaaatccac atgtcccgga gtatccaaaa
     5221 gagtaatctg ggtggaagcc atttcaaaaa cagcctgttt tgaaaaaata gtaattcccc
     5281 tgcttttttc aatttcatct gtatccagat atgtatcctg tttatcgact cttcccattt
     5341 tccctatttt tccgcttata tagagcatac tttctgataa ggtggtttta ccagcatcaa
     5401 cattggctaa gatacccatt actaattttc tttccatagg ttccaaatcc ttcattaatc
     5461 tattttattt gtaaattgtc aaatatggct acaattctta atatcctgca atagtatgat
     5521 aacaataaag atagtttttt tcaattttaa aataatatac aaaccctaaa gcttaaaatt
     5581 taaagtttta atttatgtta aagctcgttt ttctataact taaagtttct ttattaatcc
     5641 ttgttcaata aaaaatcagc cggcaatttt ctaaccttgc cggctcatcc gatatcttat
     5701 ttattaattt cccagtagca gcgtttagga gaaacaatct taccttcttc tttaagtttt
     5761 ttcataatct tatcgacttc ttttctatct acaccagtct cctgagcaac tgttcctgca
     5821 ttaacaggtt tgtctgcttt cctgaaatat tctaatacaa cttcataatt atccatcatt
     5881 accatcctcc tttgtgtaat aaataataat aattatttaa aaataattat atcataataa
     5941 tacatatttg tatatatagg taatgtaaaa tgattattat ttaatttata taattatgta
     6001 ctagtacttc aattatattt tatttttctc aattttttca attaaatttt tattaaaact
     6061 ctattgacta attagtatat ttatagtatt gtaaaaatgt ctagtattat aatgtataag
     6121 ttataactca tactttatac tttaaatata aatgagggca aagacaagtc tttgcccata
     6181 tttttttaac tcatacatca tttcatcaaa aaatattaat tctatatgcg ctagccaaga
     6241 ttaagaaatt gttatttttt attatatata tattacacta aattttatat aaagacaata
     6301 atcgacaaaa cttcttatat cctgattatt gtgcaaaata tcaagctgtt cctgattcat
     6361 cacatacgcc tttaatccat tgaaacaaat gatttataaa cactggaact gcaataacta
     6421 tgcctaacgt tgtaccaaca aaaaaaatta tactccatgc aatgacaaga gctgttataa
     6481 aagaagccac ttgtatcgga ataaacaccc aaaaggtaat gtatcaataa tttctcagat
     6541 tttctctaaa ataatcataa caagccgata aatcagagga atacctattt cattttaatt
     6601 tctattcttt acaaaggtct ccatggcatc atttctacct ttttgcaatt ttcttttaca
     6661 ggtaaattcc aaacgtaaag ataaacatcg attttttctc ctgtatccaa ataagcgctc
     6721 tctttttgcc ttatgtacat atgaccttca gcttccagaa tatctaactg ccgtaacagc
     6781 ttttcatcaa cttgatagac ttccccaaga atactttcac ccttgtcagg aactatccct
     6841 ggaaagtaag aaacatgata catgctatag ccttctactt ttgccatccc taaaaatttg
     6901 acattacttc tgccttctaa gtaatgcttg taattgctaa atcctttcat caaagtacca
     6961 taaacaaaaa cattgtattt ttgcattttc atacctcctt aattcttttt tctcacttta
     7021 ttttaaatct tcaagaactt cggcattgga cattaaatgt ccaatgtaca atttaaaaaa
     7081 ctccatgtcc tgaaagcttt cagatcatgg agttttttaa taggtataat ctaatttttc
     7141 aatccagtca attttaatca atatatattc attgttttga taaactccta ttttattggg
     7201 atctaatatt tccatataac ctatggcgac ttcctttaag cctgtatccg ggtttttata
     7261 gtgaataacg gccttcttct ggcttttttc taaaaaaacc aattcttctc ttatattatt
     7321 gctgaacatt tttctttctt cccgaaattc aggttttact tctttaaaac ttattctttc
     7381 tccctgtctg tctatggagt tttgaatgat atcttttgca tattcggtta attgactgta
     7441 tacccaatct gcaatgtggt ttaaaatact ttcatattcg cttcctcttg caagcttttt
     7501 taaacctgca gtcatggcat aaacctgcgt catattaaga ggtaaaaaga ttggatgagc
     7561 cgtagaacta taatatagtt cctgactcct ggtatttgcc cctccttttt gggatagcct
     7621 gtattctact tttaatttaa ctttttggcc aagaagctga agaccatcga tctgcaattc
     7681 atcaatatcc cggtcaatac ttttacgggt cacacaataa taatcggcta attcgtcttt
     7741 ggtcatagga gtttcctgaa gtttttttat aatatcaagc tgtctttcta ttttattttc
     7801 taaaatcctg ccctgataaa agaggttctg aattttcaac ctggaattgc attttttctc
     7861 aatgaaattg taaaaagctt taatatattg ataggctttt cttttcgtat attcattttc
     7921 ttccatatca tttttgcttt gatttaactg cgcactgtca acatctaaaa ccgtatcaat
     7981 actgtcagca agaatgatcc attctgtttc gttttctctg atttttttta attctaaagg
     8041 ccaaagctta tgcttttctg cctccttttt tactataggc atataacaat ccataagaaa
     8101 agcctggcag ctatcttttt cttcttcatt cttaagcgtc gccaggtatt tttctacagc
     8161 ttcttcaaaa gtcatcgtaa tcccccctga cttttattta taaatggtta cgcaggaaaa
     8221 tttcttttta aaaacaggat aatttttgcg atgaaaagca tccagttctt tcaaaccaag
     8281 ttcatctcta acctgcctgg gaatacatac atgaaactgc agattatctg caggcgcctg
     8341 cccattgcct aaaagcctgg aatcagtttt ataaactttt agtttcagtt ctttttcttc
     8401 atatcgaaca taaatccaat ctcctatatc aacagataat aaatttctta aactttttgg
     8461 catgcctata acataagtgc ccatactgtc cagatagctt gaactgtcat atacttctgc
     8521 ttcataagat actttatcat tatcatttct ttttaaagaa gcaatatgtt ccagcaagta
     8581 cttgttaatg tattcttcag gaaagttcat gtttttcatg gcttcataaa caatctcttt
     8641 ataggattcg gatggctcta tttcatgctc cagtttattc ttaacaaaat aagtatatgc
     8701 tttaattttt tcaccattta attcaatttc aataagatct ctgtaataag cccctcctga
     8761 aaccccttct cttcggtcaa tgacttttat taatgctttt tcaggaacct ggtataccac
     8821 tcctaaaaca taatccccca aagaaggaat catatccagt acacccccgc ctcttgtaaa
     8881 agcatatctt gtaaaagcca gtcggaaacc ttctaattta ccaacaccca atattttata
     8941 agatttttct aagtgtgcct tttttaagtc ccttatgaaa tctttttcat tcatacaact
     9001 tccataagca aaataattga cgactgccat ttcgattcct ccttacctat attccttgtt
     9061 taatcctcta ttgattcggc ctctgccata aggttctctt ttaataaata ccccataatc
     9121 aggcggttgc ttggtaggat ttaaatattc ttctgctacg ggataatcaa caaaaacttt
     9181 cgtatcgact atgatagaat ccaaagaagt caggtaaact ccgtcccggg catcataaac
     9241 ctgaacctcc atgcgataat ccagttccgg tataatcaca aaacccacca gatactcctt
     9301 tgtgccctgt tgactttcta aacgaacata tctggttaaa tcatctactt ttaacacttc
     9361 ataaaccggt ccacaaaaaa gtctgtctat aatcaaatcc caatgaatat acttattagg
     9421 atttatccct aaataagccc tgtaatccct gatctggggg gctataacac cttgtatggc
     9481 gtataaatca agggctgaaa gacatcttat ggcattttct gcatacaagg ccaattgcca
     9541 ctgtccccag tcttcctgat attcaaacct gagacaataa gctttttcaa ctccactgta
     9601 atacctcaca tagatatatt gggtcatgga atcaatttct tctgaattta gtattttaaa
     9661 gccatactct tcttctgcca tgataaaaaa tgcattccag ggcacttcat ttaaatgaat
     9721 atacatgaaa accactcctt ttacttcgtt tgattaaagt ataaaacagg agtggtctca
     9781 aacctggaca tctgatgtct aagctggctt tatcctttct atataatatt gggaacaatt
     9841 ccattcttaa tttgactgcg gtaataaaga ttgacgccaa agtaaaagag ggcatttgcc
     9901 ggccgtgaaa ggaaggtctt cgtcagcagg gtccttttta cgatgttttt tatactgtaa
     9961 acttcattat acaaatccca gtaggctttt gtaagttctt ccggcgtcat gttttttgga
    10021 atatggacgg cttcacaacc attataagaa tagatatctg tattatagat tcgctttgcc
    10081 ttttgcatct cttcaaaata tttagtcccc ggaatggggg ttaagatata aaaacgcgga
    10141 acagcaatgt gattatctga aataaactta gccgtatttt ttatgctttc taaagtgtcg
    10201 ccatcagcac ccaccaccat ttcagtagaa atatcaatgc catgctgacg gataattttt
    10261 atctgccggg cataattttc aggctttgcc caggccttat tcatactcct caggctttct
    10321 ttgctgatac tttccagacc aaaacttaat acataacagc cgctttcagc aatagctttt
    10381 aagagttcct catccgacgc aatatcaata gaacactgcg tcatccattt cattttcaat
    10441 ttttttattt ccgcacatag attcagggca tattctctgt ttgaaaaaat attgtcatct
    10501 aataataaaa actgtttaaa acccagctct ttgactcttt taatatctct tataacttca
    10561 ggaatttctc tttttaaata ttgaccgcgg tataaacagt aaaccgaaca aaagctgcag
    10621 gctttcgggc agccacgtcc cgcctggacg ggcaggaagt ttcctatttt tttatttaat
    10681 aataactcat atttgggtaa aggcgttttt aatgctttaa gctttctttg atataatttc
    10741 ttcagcttgc cttcttcata gtcctttaat agttcaggcc agctttcttc tgcatccccg
    10801 ataaccaggg catcacagta cttttgcgct tcctccggca ttaaactgac catatagccg
    10861 cccaaaacaa cagttttacc cagttcttta aacttttttg caatatcaat gcttcgtata
    10921 acggcatggc ccatactgct tatgccaatt aaatcggcat cggtatcaaa aggaacctct
    10981 tctatagttt cataacaaag ttccagttca tactctttgg gtgtcagggc cgccagtaag
    11041 ggaagtgcta aacctacaaa atataacctg ctttttttaa taagattgcc cttctgatcg
    11101 taaggggtag gctgaatgag taatattttc ttcttcattg ctggctcctt ctttgaatat
    11161 gctcataatg ggcaatatga atgtcttcaa ttcttaccag aagcccttct tttgtattat
    11221 aaaccttatt aaccaggcca taatgggtta accttaacat acaaaagcca ggcataaaag
    11281 cagaagtcct gcctacttca ggaggataaa taatctgctc tgctattcct tcaaagccca
    11341 ccattgaata ggctttaatg tctttaactt ctttccctct tgaatacttt ggtccaacca
    11401 cccaggtata taaaagtcct gccagttcat cgacttcgta aactttaagg acatgggtaa
    11461 ccggacagcc tgctcccaca ggtctggcaa ttacaatatc accagcctga atatttaatt
    11521 tttcgattaa gtccacacga aagggatgta taatttttct tgccgaaggt tcatcagcaa
    11581 tagcttttaa gacaaaatca taaacattat tgtggatatc cagatgatct tcttccacaa
    11641 tcgtttcctt ataaacatta ttgttattat aaaaggggca gtcgtttaac cgcctttttc
    11701 cattttctat gctcagtctg aaatccttac aagttctctc accgcataaa ccgcaatttc
    11761 tatcttcctt ttcccaggtc aaatttaatc cccccgatac atgtaatccg ctgcattgct
    11821 gtcgatttgt cttactacgc cataatgatt tttccagcca atttttttgc tgcctataca
    11881 aatggtacaa gtccctaaag gtggattacc ctttaactct aagttgccta aatgaatctc
    11941 ttcagactca tcaattaaat tacataaata ttctatggca attccctgaa gggcattggt
    12001 ttctattact tttaagtcat taaaggaatt ctttaacttc ttaatgtaaa cttcccgttc
    12061 tgcctgacag accaaatcat tcatggttaa aaccgcaaca tcagcaaagc aaataagctg
    12121 tttcatttta tcaacagaat ggatccctga aagcatgctt aaaaccacta aacctaaacc
    12181 ctgatttaaa taaggggaac atcttaaaca taaacctgca ctttctatta tgagtaagtc
    12241 tgcctttaaa tcttcagccc atctaatggc atccgtaagt atcatcacta ctgcatggtc
    12301 cggacataaa tctcctgcat agacggttct tgcatgaata ttgtactttt ctttaatcag
    12361 ctcttcctca tgggcataga ccacatccat ttttaaatag gccatcttat attttccctg
    12421 tcttttttca ataattctct ggatgacggt ggtttttccc gtagatggtg gtcccccgac
    12481 taatagcaat ttcataatct catcccctgt attatttaaa aatctatacc atttcgcagt
    12541 ttatcctgaa tttcttttat ggttttatct gccttaagaa ccagagccaa aacttcttta
    12601 tcttttttgc tatactcagt gattttttca atggcgccgt ttttcattag gattcttttc
    12661 tcggtttgta agacaatact tgggtcatga gtagcaaaaa taacaagttt attttctttt
    12721 ttaacatgtt ttaagatttc tgtcttgttg atgcctgcat tttctatctc atctactaaa
    12781 ataattggag aagccccaat taaaatggca tcagcaaaca ataaagctct agtctgtcct
    12841 ccagataatt ccgttacctt agctttttca gaaatggact ctcctgtaaa tttattcgcc
    12901 agatcaatga cttcaccaat aaggtctttg ttttttatct ttcttgaatc tgcatgaatc
    12961 ttaaggaatt cccataccag taagtcagaa acacattttg tattttgtgt aatcatggct
    13021 atctttttct tttcaggatt aaaacgctct tcataacccg gaaccttgtc atcaatggtt
    13081 atcattcttt tggaagcact gtctctttga gacaattgtt cgatatcaga aattagcaaa
    13141 gttttaccgc tgcctgtatg gccaacaatg gaaatgactt ctccaagttt aaaagtaatt
    13201 ttatcatagg gttctttttt cccgctttta tcaaatcctg cgtaaatact aatctctctt
    13261 tccatttcgc tctccttaaa acagctatta tccttttact ttaaccttat tccggcaact
    13321 tcttaggatt aaagatattc tttatgtaaa ctttaagtat ttggtcaaag ccaaacatta
    13381 aaccaatatt tcgtttaacc gggaaagtcg taaaacaata tttaatagtg caccagttaa
    13441 agttagctgg tataccaagt tttaagactt caaaataata tcttctgagt gacattttag
    13501 aaggataaat aatcacatcc ccaaaactcc atttttcata atcatctctt ggataaataa
    13561 gcctgtcttt atattgctta taaagaggtg tcagaaaatg aggaactaag ggcgtaaaag
    13621 aaatcaattc aggttttaaa aatttaaggt atttcttaaa agttttaaag tcttcttttg
    13681 aaaagtctgg atgcaaaata aaggttcccc aggtggctat gccgtttttc tttaaaattc
    13741 gaacagcttt atagttatcg tctaagtttg cactcttgtt atattctttt aaaaaggcat
    13801 cggtaaagct ttcaagccca acgataactg cctttacacc attttcagat aagcgcttaa
    13861 aaagatattc tttttctaaa atgctgttaa cgctgccata acaaatataa gtctttttaa
    13921 tttttctttc ttcaagtaaa tctaaaaagc ggatgagtct tttttcattg atcaaaaagt
    13981 cactgtcaca tatcatcaca tagtcttctt ttaattcttc tatttctgct acaacgtcct
    14041 caataggtct ttcctttaaa ccaggtcctt caagctttct tctgatacaa aagctgcagt
    14101 gattcctgca gccataagaa gtctgaatgg tcgcataagg ctgatatccg gcatatttgt
    14161 atttgtgcct gtattttttt gctgcatccc tgtcgggctt tacatattca ttttcacaag
    14221 aggaaatagt tgactggaac tggcgcttct tactgaaaac acccattatt tcttcatccg
    14281 tatccttttc gatttgttcc ataagagctt tcatattttc tctggtcgtt gttttgacaa
    14341 cataatctac ataccggcta aagtaggctt ccggggtaat ggtgacctgt atcccgccta
    14401 caatgactgt aatcttatgg ttaatttttt tcgttttttc agccagatat aacacattgc
    14461 taatgtcact gcattgagaa gtaaagccca caacgtccgg cttaaagctt tttatgtatt
    14521 ttctgagatt tcttttggat tcggctaaaa aatcaaccaa aaggacttca tgcccttgct
    14581 cttttaatat agctgaaaca cattccagtc caaggggttc cccaaacatc agctttttta
    14641 tagtggttac ggttttatta agatgtggtc ttattaataa tactttcact gcctcatcac
    14701 ttgcctccaa ttaatttttt aaaatactgc agattaatct taaaggcgcc tatacttaac
    14761 tttatggtat catgcagacc gtattttctt atcatatacc agacactttt aggattaagg
    14821 gtaattttat aataaagtaa gatggtataa taataatatt tccttatgga tatatgactg
    14881 ggttttacaa ctaaatgggc caggtcccat ttttcatatt catcttcttt tattataaaa
    14941 ttccctttat actgctcata taattccgtt ccctttaagg gcgttaaagg ctgaagatta
    15001 acaaaaacca accctaattt tttaatccat ttggccagag ccataaagtc ttctttttcc
    15061 cagtccaggc ctaaaatcag ggttccatag cattccacat catatttttg caggatttta
    15121 acggctcttt cattagtttc tacctgggtt tttttattat agtctaaaag ttcattgcta
    15181 cggcaggatt ctaagcccac aatgactgcc cgcaaaccca gggctttaaa tctttttatg
    15241 acatcttcat tactggcaac aaaatctgcc ctgccataaa ttaaatatct tttttcaatt
    15301 ttattttttt ccagcaaatc acaaaatctt aagattcttt ccctgtcaac taaaaaatca
    15361 tcatccacaa tatagatttc tttttcctta atggacttta attcttctat gatattttct
    15421 aagtcccttg taaagtaatg tccatctgta acttttctgc aaaaacaaaa tttacactga
    15481 tagggacagc caaaagagct ttttatcaaa ctacaatttc tgtgaaacat ataataatac
    15541 ttggacctat attttttaac cttttctctg tccggaaaca aataattaaa ctgcttttct
    15601 ttttctgcct tttcattttt ttgatgatat ataccttcaa ttacagaaac atcttcattt
    15661 ttttctaatt tgtttaagat ttcaataaaa gtccttatac cattagcccg aacaatataa
    15721 tcaatatcct ctgataagaa atcttccgga ttaacttctg catgaacacc gcctacgaca
    15781 attttgattt caggattaca ggcttttatt tgctttgcat atgttttaat aacattgata
    15841 tgggtattat aacctgtcat cccaaccaga accggctggt atttttttat aaagtaagtg
    15901 actttccttt tttctaaaat catatctaca ataactgcct gatgaccaaa ggcttcaata
    15961 tttgatgaaa gatactctaa ttctaaaggc tcacaaatca tcacattttg aagaccaatc
    16021 gtttctttat ggggcttagg tcttattaat aatactttca tggttgcacc tctaaatgaa
    16081 ataaggcaat ggtaggcata taaaaccttt cccttataat ctttattttc ttaactttca
    16141 accgggctgc ttgaaatagt tttttcattt cttttatgga aaggtatctt gctttacttg
    16201 ttgtggcttt taaaaagaaa ttaataatca aatccttcag attatttgtt gaagaattga
    16261 caataatcac ttgcccatga ggattgcata aagcagcaat cttctttaga gtttcttctt
    16321 tattggggaa atagggaaaa gaatggctgc aaataatcag gtcatacttt tcctgacacg
    16381 taaaattttc tacaggacaa actctgaatt ctatattttt ttccttgtta tttgactttg
    16441 caatttctat catatttttt gccacatcaa ttcccagata ataaagctct ttatattgac
    16501 tgcttatttc accaattaac tggcctgtcc cacaaccaat atctaatatt ttaagattgg
    16561 ggttttcttc tagcataggt aaggcaatct ttaaaatttc ccttctggtt ggccctaaag
    16621 aatacttctg aacccaaagc ctgttatatt tatgtgctaa tttctcccat acctcgtatc
    16681 gcatcatttt tcccccatta tccttttaat acctcaagcc agtaattaaa agaggcccca
    16741 atagctgctt taatatattt aaaagataaa attctatttc tcaaaattaa tagagcaagc
    16801 ttaacatata atttataaaa ttccttgtaa aaagttaatt tactcatctt tgcaggctca
    16861 ataatcaaat gaataaagtc ccatttccta tagtcatcgg ttataattct gtctttatat
    16921 ttttcaaatt gctgggttcc cggcataggt gtcagcacgg aaatggtagc aagatataat
    16981 tcatgttgtc tgataaagtt atagagcttc ttaaaataac ttttatctgc atcaatatct
    17041 atcatcagta aagcaataca gctgatcttc gcttctttta aaacctccag ggctctctgt
    17101 atgatatgga cattggtctt tttattatat ttattaagaa tttcatcatc aataacttct
    17161 aaacccactg cacacatggt tacaccaatt tctgcaagtc ttttcatgta ttccggattc
    17221 tgggcaataa aatccgcccg gtaatataat gaaaactttt tttggagctt ttctttttta
    17281 gccagttcaa tgaaacggtc taatttttct ttgtctacat aaaaagtgtc atctacaatc
    17341 caaatatttt cacagttaat actttttatt tcttcaagca gattttcagg ttccctgcaa
    17401 agatatcgtc cgccatttaa tagggtagaa aaacaatagt tacatttatg aggacaactg
    17461 tagctacctt ttacaatagc acagggtttc atggtcacat atttatattt ttttatattt
    17521 ttgtaaaaat gactcctgtc cggaaaaggc aagctttcaa tatcaaaaat ttccttctcg
    17581 tttttatgcc agtcaccatc cttataataa taaatccctt ttatcttttt aaaatctgtc
    17641 ttattattgc tttttataat ttcttcaaaa ggcttaaagc cgccactata gcaaataata
    17701 tctatcccct caaattgaaa gtcttctggc atcacttctg catgaggacc acctaccatg
    17761 gtcataatgg aaggattaaa ttttttaata catgcaacat atttttttat agaatggata
    17821 tgtacaaaac tggccgtcat tgctactata tccggtttaa aatgatttaa catatatttt
    17881 aaggatttat cttttatact caagtcacag atttgagcat ctatatgatt tcttttacag
    17941 atagcagcca tatattccag ctccagaggc tctgtcgtaa ttaatgataa ttttgtaaat
    18001 acgtttttaa attctggttt tactaataat accctcattt tttatccttc cttaaatacc
    18061 cggtattggg gaaaatcata tttgtattct aagaagtctt ttccgatatt cactaaaaaa
    18121 ttaatgatgg gattggaatg atgggcataa aaatatcttt tttctaataa tgcccccaat
    18181 ttcatctttg tttcttctga ggtctgacca aaatcaatga ttttgcactg cctgtcaatg
    18241 gcatactcaa taattttata gagcatataa taatataaat ctgctgtgtt atagcgataa
    18301 tccataccgc aaaacataaa gattaattta tcttctactt tttttaaaag aacaaagcca
    18361 atcgcttttt tgtcttttaa aaagactatt ttggtaccat caaaaacttt aaaaaaatta
    18421 ctttctaact tttctaattt ataatccgac ttgttgtagg tgtttaaata taagtcatag
    18481 acgtcaatcg tttcatcatt aatctttatt tctaaatctt cgcagttttt gatggcttta
    18541 ttaattcttc tccggtaaga acttcttaat gcagtcaagt attctgtcac agaatgaaaa
    18601 cgattataaa aaacacaagt cggtaaggtt tgccctacgg tgataccctc tgatcctgtg
    18661 ggatttgaaa catttaaaac caactttgct cctttaaggc ttttgatata atccagcatc
    18721 attttttcat tattagtcac atagcctgca ttgctcaaag aacaggggta accaattact
    18781 ttaagtttca tattgaactt taattttcca aaggtaaaaa tgttcatttt attcttatat
    18841 aatacaaaaa acgcataatc ttttccctgc tcaagataat aatattcctg ttcaaattta
    18901 ttttcttttt ttaataaacg gagcaagtct ttctcaaagg gatacggcaa ttttatattt
    18961 aacttaattt tattaaaatc atcgctgtga taaatcttta aatgtttaaa ttgctttttc
    19021 ataaaccaca taccttttat attcttcatc acagatggct ttacaaacac tgtttgaagc
    19081 ataattttct tccagaaccc atgaagtttc tagactttta attttatatc tgtcaaggca
    19141 taacttgatt tgatttaata aacctaaggg caagcctatt tttctataat cttctaatag
    19201 ggcaatttcc atgactttag ctttttttat ttttttatta aataacttat ttttaaagta
    19261 atgcttcgta ccaaaagcgt ctcctttttt taccaattcg ttgtaatcct gataccataa
    19321 agtgaatcct acaggctctt ttccatcaaa aacaaaaatc aaggaatcat ttttcataaa
    19381 taagaataac tctttaagca tttctttatc ttcttcataa ccacgtttat aataatacct
    19441 gtgattcata aaacattgat tatttaagtc agtatagatc tttgaataat aatcaaaatt
    19501 cttcttatca aaatactcaa acctgtattt tttattaagt ctgtctataa tcccttcata
    19561 gacttttaac ctgtcatcaa tacgatctat cttatatgtc tttaaatgca cttcttcaaa
    19621 gttcattttt ttaaagtagt cgttataata ctttggattc gcactggcgg aaaaagaatt
    19681 cttttcatca tagtgggaat ttaacagacc taaaccataa tttagatgac catttaagcc
    19741 tacaactaac tttttgcaat gatacctttt accaacttca acagccttat ccactaaaag
    19801 ttctactgcc ttttcctgat tggctttgct ttcaaaaaaa cacagttgaa tatattcagc
    19861 taattcctgc gcatgaataa taagaccttg acataatacc ttcccatcat cttctacatt
    19921 cacaacatat atttttttat tattagaaaa acaagtttta ttggcaaaca acatttttag
    19981 cataaacata tgattgtcta caaaaaccgg attggactga tatatctttt ttctaaaatc
    20041 taaaaacttt ttctggcctt tcttactttt tacttcaata actctcattt tacaatcctc
    20101 tctacagact aattcttttt tgaaatattt ctcttaaacg caataaatga caaaaaagac
    20161 aagttcctaa tatggaaatt gtaccaataa agtgtcaaaa atacaatatc aaacagcttt
    20221 ttaaataaac ttttatttaa ttttaaaaca attaattatt tttactctcc taatctttgg
    20281 tccttgcatt aaatctgctt taaaaaaaca aataataatt ataataacat tttaatataa
    20341 tataataacc aagttttttt atctttttaa tgctgtttcc cttgtaatga tggcttttct
    20401 ttataaaata caatctttaa cagtttaaat aattaaatta agtaattgac aaggtcattt
    20461 ttctaaggta aaattgaaaa aaattatata agaacttctt aatggctatg atcaagagct
    20521 agtagcagta atgaaaatcc ttcagagagc tttcggttgg tgtgaagaaa gcaggattat
    20581 gcttactgtg aattcgtctt aggagctgtg tactgaacgg tattttttaa tacccagtag
    20641 gctgcaccgg aatccgtccg ttaccgcgga aaggcaatga tagttgcctg gtgaggttgt
    20701 gcaaacaacg aagcaaggtg gtaccgcgac caacaatcgc ccctgtaatt ttacaggcgg
    20761 cgttttttat ttaaataata aataataat
//
LOCUS       DAOSUD010000038        20462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1700_length_20462_cov_20.155927, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000038 DAOSUD010000000
VERSION     DAOSUD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 20462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20462
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_20462_cov_20.155927"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLG49_06285"
     CDS             <1..226
                     /locus_tag="PLG49_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ16968.1"
                     /translation="TPGLFYLSFKYGDKEEWNEGRYFRYHNEKSFEKIISKHKEFEIL
                     KMSKSKDRVKIRKDKEGEYWLNVLLKKRHD"
     gene            400..744
                     /locus_tag="PLG49_06290"
     CDS             400..744
                     /locus_tag="PLG49_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HOQ16969.1"
                     /translation="MPFISTRTSVSIDAEKRENIKAKLGKAIELIPGKTENWLMLSFQ
                     DNLPMYFKGSNQEPLAFVEVMLFGSASQEAYDKLTKAVTDILNEELGIKPNGIYVKYE
                     ECKHWGWNGSNF"
     gene            complement(874..2364)
                     /locus_tag="PLG49_06295"
     CDS             complement(874..2364)
                     /locus_tag="PLG49_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ16970.1"
                     /translation="MIIIKSILKNIKEKKFRTFLIILSITLSSALFLASITNSKNMVE
                     VRRKQIKAQMGTADIYFSKNLRADNYFFSTNLLKQYKDHFEYIIPGINSIAIYEDNNQ
                     SKLSISMLGIELEDLEKLNPISLAESKNLYPFSGKKVIISEKTTQKYHLSLGDSIKLK
                     INNSHYYFTIAGIAHGDGFFFQEIGMIYAICPRDCMEKINGALGQSNILYLKLKDPDE
                     IISTLHWLSDKYYDFDVREPYTLEDIKQEIRGIEVSFKLITALVLSVSILIIYSSFQV
                     IVLERLPIIGTFRSIGATKKYTSFVLIMESLVYGIIGGLLGLLLGLLFSYILVYGTAP
                     DWVRQGGIQLDISVFHMILTFILALFLSAASSIIPIIQTTKIPIKNIIFNLLEKNKEK
                     KISHLIIGCFLLLFIWMSSYFCPKAYLFEISLLNVILSIFAEILLTPFIVFSICSILE
                     KLTPKIFGNLAFLAFKNIKESKSSNNNIILFSLGISSLLAIEKQAI"
     gene            complement(2472..3719)
                     /locus_tag="PLG49_06300"
     CDS             complement(2472..3719)
                     /locus_tag="PLG49_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ16971.1"
                     /translation="MIDDYDKFYEKLYPYLSKVEGRLQVIDKNGFLLFDSEDKKTSLE
                     KKKVYIENSFGYDPNYHLQHPGFHRHNSPIFYNNELVASAVITKDMRHFGNGIQYKIN
                     LYYQIAAITTPVLLIVLILLFTVYLSRSILIPLKELNRATQNIAEGNLDFKVSYKRND
                     ELGEFCRTFEEMRFKLKESLNKQIFYENSRKKLIACISHDLKTPITTLKGYIEGLQDG
                     IAKNEEMFNRYLQVIHNEINSLDRLINDLFTFSQLETDKISIEKEEWMADEFFKDLLE
                     AVIIKTENQNRMLEVPKMIPSVKLMIDPQRIKQVIDNLINNAIKYTKTKGLIRIETEL
                     KDSFFIVSIQDDGTGIPQEDLPYIFDYFYRAEKSRNEAYGGIGLGLPICKKIIEGHEG
                     NISVKSSPGKGTTVIFELPIVQE"
     gene            complement(3712..3912)
                     /locus_tag="PLG49_06305"
     CDS             complement(3712..3912)
                     /locus_tag="PLG49_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16972.1"
                     /translation="MKKPLKIQSKIFMAFLAVILIPSLCVFIPFSLLYNFNNDVKDLN
                     NINQLLYDTFAKVQENYDMIDD"
     gene            complement(3909..4610)
                     /locus_tag="PLG49_06310"
     CDS             complement(3909..4610)
                     /locus_tag="PLG49_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ16973.1"
                     /translation="MAGEKILIVDDKKEIAELIRDYLENEEYEVELAYNGQEALELFQ
                     QFKPDLMILDIMLPVIDGLEVCRIIRSHSSVPIIMLSAKKSDIDKILGLGLGADDYVT
                     KPFSPSELVARVKAQLRRYLQFSEAHHNQDQLVYPPLKINLKSYTVYLEDEKLNLSTK
                     EFEILKLLALHPNQVFTREQILDKVWGYNDYADLHTVTVYIRNIRKKLEKNPQAAEFI
                     QTVWGVGYKFEGGQS"
     gene            complement(5091..5522)
                     /locus_tag="PLG49_06315"
     CDS             complement(5091..5522)
                     /locus_tag="PLG49_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ16974.1"
                     /translation="MKNQYNLPCNIAQTLNIIGDKWTLLILCQIFKGNDTFNALLENL
                     EGISTNLLSNRLKALEKDELIIPVLYQKHPPRYRYELTESGKDLEDIFYSIILWGERH
                     LKKCYKEVIHQSCHHKIQLKYYCPHCDKMINKNELFIKDIE"
     gene            complement(5612..6910)
                     /locus_tag="PLG49_06320"
     CDS             complement(5612..6910)
                     /locus_tag="PLG49_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOQ16975.1"
                     /translation="MDSIVSAGNISFFLVFIEGIVSFFSPCVIPLIPLYMSYLAGSSN
                     ETSENGKMIFDRKKVFINTIFFVLGISFAFFILGMTFTAVGSFLNSNRLLFTRIAGIL
                     VIILGLFQLGLFNIKFLNQERKIHLNIDNKKVNPFVAFLLGFTFSFAWTPCVGPALSS
                     VLIFASSSNSYIIGNLLVLLYTLGFIIPFLLLGLFTTQVINFLDKHRNIVKYTVKIGG
                     VLLILIGIILFTGWMNSISSYLNSFGSSRTPNQQEEISDSSTDEKNEENQETNEENQE
                     TNEETIYPAIDFTLVDQYGNEHTLSEYKGKVVFLNFWATWCPPCRAEMPDIEELYHEY
                     GENQEDVIILGISSPKTAENPYTKEVDKEGVIQFLDERHITFPILFDETGSVFYDYEI
                     YSLPTSYFIDKEGNIYGYAPGMITKDVMKSTIELLRNPVD"
     gene            7188..7844
                     /locus_tag="PLG49_06325"
     CDS             7188..7844
                     /locus_tag="PLG49_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ16976.1"
                     /translation="MDIFIKHQLDKDASGYILTLYINPDLTEFAKELTNKEDISTNKN
                     FYYSIKNYISENFKNIKINTIKLMAGSMLIASIPFTSPVSVSAAATGVQSSQSSSPIS
                     IIINGQKYQFSRSPVIKNGTTYIPLRDVAETLGAEVWWNASSKTVGINKDNTKISFVV
                     GSNKVRVNDRLIEMQPAYIDNGTTMVPLSLLVKVLEWMFTGIKIQKQLTSVHPLNRIK
                     "
     gene            7775..8596
                     /locus_tag="PLG49_06330"
     CDS             7775..8596
                     /locus_tag="PLG49_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ16977.1"
                     /translation="MDVHWDQNTKTINIRSSLKSHKVKAGESLWKIANQYNTTVSKIK
                     ELNALKGDTIYAGQELKIETDTNAKINTPSSSNYITYMSYSLKAGDNLWDLSIKYGVP
                     FTELLAVNNMTENSPLSVGQKIKIPVHHIAQKERVSERHGEYLDWWTEAQYLFPIDKV
                     ATVTDFQTGKSFKVKRTTGAFHADCEPLSAKDAAIIKEIWGGNYSWKERAVIVQVDGR
                     KIAASMASMPHDVACITDNNFNGHFDIHFKNSIRHKDGLISTAHQEQVKIAAGIR"
     gene            complement(8641..10068)
                     /gene="hydG"
                     /locus_tag="PLG49_06335"
     CDS             complement(8641..10068)
                     /gene="hydG"
                     /locus_tag="PLG49_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOQ16978.1"
                     /translation="MYNVKSFDSNEFICHEEILNSMEDAKKAVQNKYEIERILDKAAN
                     LKGLSHKEAAVLLEVDDDETLNKIYKIANDIKEKIYGKRIVLFAPLYLSNYCVNNCKY
                     CGLKCSNQIARHKLTQEELREEVRALEAMGHKRLLLEAGEDDQNCPIDYILECIKTIY
                     NEKFKNGSIRRVNIDIAATTEENYKKLKEAGIGTYTLFQETYHKPTYLEVHKGPKRNY
                     EWHTEAMDRAMLGGGIDDVGIGVLYGLYDYHYETIALLMHVEHLEAVCGIGPHTISIP
                     RLLPAEGVNYEEFPYLINNRDFKKVVAVIRLAVPYTGMILSTRESAEFRNELLTVGIS
                     QMSAGSSVGVGGYAKKDVYIEDGVEEKPQFYLADHRKPIDVIKGLVKDGYIPSYCTAC
                     YREGRTGDRFMPLAKSGQISNCCLPNALLTFEEYLRDYADDELMEMGQSLIDKEINNI
                     PSERRRQRTLEYLNRIRNGERDLRF"
     gene            complement(10099..10356)
                     /locus_tag="PLG49_06340"
     CDS             complement(10099..10356)
                     /locus_tag="PLG49_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOQ16979.1"
                     /translation="METRIAIVGIIVENPDSVEKLNKILHDYNEFIIGRMGIPYHKRN
                     ISIISIVMDAPNDVISALSGKLGMLPDVSTKTVYSKLSAKK"
     gene            10688..11494
                     /locus_tag="PLG49_06345"
     CDS             10688..11494
                     /locus_tag="PLG49_06345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HOQ16980.1"
                     /translation="MNILVTVDSNYILPLKVMLKSLFLNNAEDEFTIYLIYSRIKEDE
                     LADLNTYIKQHGRQLKAIKIADDYFEDAPTLMHYTKEMYYRLLAFKFLPEELDKILYL
                     DPDILVINKIDGLYHLDLEDYLFAAAYHNKISVREINRFRLSPYEIDKYYNSGVLLMN
                     LKLQRNLMNENEIFDFVRKHKSKLIMPDQDILNALYSKKIKSIDEILYNYDARFYNYY
                     KILSSGEYDMDYVIYHTVILHFCGKKKPWQKKYSGKFHSLYKHYEKLALS"
     gene            11858..11977
                     /locus_tag="PLG49_06350"
     CDS             11858..11977
                     /locus_tag="PLG49_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16981.1"
                     /translation="MRLLGELSFAEIGEIMNKSESWARVTFFRGKQQLMKGEI"
     gene            11977..12321
                     /locus_tag="PLG49_06355"
     CDS             11977..12321
                     /locus_tag="PLG49_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOQ16982.1"
                     /translation="MSLKCHIVKDLLPNYIDHVTSQETNQDIQEHLKDCEDSKKIYDE
                     MNYKITDQLIDLNQVNNNLKEINYLKKYKNTKILFFVLSYSCLLLLEYIQCFLLSLGI
                     CGCMIVRKRLMI"
     gene            12288..12800
                     /locus_tag="PLG49_06360"
     CDS             12288..12800
                     /locus_tag="PLG49_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16983.1"
                     /translation="MYDSKETINDIRMYEEILGHDGKYKYHFNRYKDIFSDEITDSMK
                     VEKFCYIYYNPWDASYLGYLVYTCDDESYRKEYERLKNIKSSDNYLIYGAKEFPYELY
                     AVYADPYYGYIYAMTNPERNQFIYVELQFYNYFSEIDNEKFIDKEHLPLGFDAKWHNK
                     IQKEFLEGSW"
     gene            12842..13273
                     /locus_tag="PLG49_06365"
     CDS             12842..13273
                     /locus_tag="PLG49_06365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ16984.1"
                     /translation="MKIEKVFSRHLTDDGKFIRKKVFIEEQGFKDEFDEYDCDEKAIH
                     LVLYDNQQAVATGRLFTKDSQKKTYTIGRLAVIKEYRKLGLGKVLVQSLEEKAKEEGA
                     EKITISAQCRAEGFYKKLGYTSSGDIYYEETCPHIHMEKIL"
     gene            13270..14001
                     /locus_tag="PLG49_06370"
     CDS             13270..14001
                     /locus_tag="PLG49_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ16985.1"
                     /translation="MKVRKIAANAVAFSYENIEDFVTNVFLVETPSKFFVIDTYCGSD
                     AMKPVLERINHSSKNKEIIVINSHFHWDHVWGNASFIGKDIISHEICRKFLDEQWEKQ
                     MKHNRQYILGRAEKQLPNITFKEKIIFYDEGIELFYSPGHTIDSISLFDHNEKVLYAG
                     DNLEKPIIYVENNDIKTYIKSLKNYLNYKPKKIVAGHSLDLKEEDIFSAIEYLKGLSE
                     GRKFTFKTDYERKIHYQNISMINKK"
     gene            complement(14119..15648)
                     /locus_tag="PLG49_06375"
     CDS             complement(14119..15648)
                     /locus_tag="PLG49_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOQ16986.1"
                     /translation="MLSKVKSCSLYGINGYLVDVEVDLSEGLPGFDLVGLPDSSVREA
                     KDRVRAAIKNSQLLFPVKRITINLAPADTKKEGSAFDLPIAVGIVSCNLSISPEKLEK
                     TMIVGELSLDGSVKPVNGILPMVYSAYKNGLEQCIVPFENAEEGAIVEGIKVIGVHNL
                     MEALEYLLGTKSIPETTVNIQTLMQQSSNSKLDFSDVKGQAHVKRALEISAAGSHNLL
                     MIGPPGSGKTMMAKRLPTILPDLTFEESIEITKIYSVSGLLPDKKSLITNRPFRSPHH
                     TVSTSALVGGGRIPRPGEVSLSHYGVLFLDELPEFNKNVLEVLRQPLEDREVTISRVN
                     ATLTYPASFMLVCSMNPCPCGYYGDEEKCHCQSETIKKYLAKISGPLLDRLDIHVEVS
                     PVKYKDLNTKEKSESSASIKKRVLKAQKIQQERYKNENIYFNAQLGAAQIEKYCILRK
                     EERELLRTAFEKLNLSARAYHRILKVARTIADLDEKENIELQHLSEAIQYRSLDRKYW
                     A"
     gene            15860..16981
                     /gene="dprA"
                     /locus_tag="PLG49_06380"
     CDS             15860..16981
                     /gene="dprA"
                     /locus_tag="PLG49_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ16987.1"
                     /translation="MEGLLESTIYWFWISNLKGLKKSRIQQLLKHFKTPKNIWKLGKN
                     EIKSVINISEEELNILERSKNYEGLIEALFDLKEKNIFFYSIQDEAYPVMLRNIPNPP
                     PGIYVKGQLPAFEKGYIAIVGSRNCTEYGKNVVRELGRELVKRGIGIISGLAYGIDSE
                     AHKVALEFGGNTVAVLANGLDNCYPPGNINLMKSIEVTGALISEYPPTTKAIPSFFPA
                     RNRIISGLSKGVLIVEAGEKSGALITADLALEQGKEVFAVPGSIFSSTSTGTNKLIQQ
                     GAKLVTKVEDILEEIKLNMDIYNKYNNKEDKPYKNESISGLALEENIVYDCISFEATS
                     MEYLSVKTKYNIDKLQAILTLLEIKGLIKQLPGKKFIRI"
     gene            16997..19057
                     /gene="topA"
                     /locus_tag="PLG49_06385"
     CDS             16997..19057
                     /gene="topA"
                     /locus_tag="PLG49_06385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ16988.1"
                     /translation="MASYLVIVESPAKAKTIKKFLGKSYKVEATMGHIRDLPKSQLGV
                     NIEEDFEPKYITIRGKGEILSKLRKIVKTADKIYLATDPDREGEAISWHLMHALNLDE
                     TKTKRITFNEITKNAVKNSIKEARDIDMNLVDAQQARRVLDRIVGYKISPLLWKKIRK
                     GLSAGRVQSVALRLICDREEEINNFVPEEYWTIEAKFLDNEKRAIESKFYGLKDNKIE
                     IDSKEKADKILSEIKKQKFKIEEVKFGERIKKPVAPFTTSTLQQEASKYLGFSTTKTM
                     RIAQQLYEGVDIEGEGTVGLITYIRTDSVRISEEAKAQAKKYIETHFGKEYINTREYS
                     YKNKGKSQDAHEAIRPTYIDKEPKSIKNSLSNDQYKLYELIWQRFMASQMAPAAYDTF
                     SIKISAGDYIFTSSGSSVKFDGFLIVYNKEKEKEEEMNFSTNIDEELKLDHLEAKQHF
                     TQPPARYSEATLVKTLEENGVGRPSTYAPTISTLFNRGYAVKENKVIYPTELGEIVID
                     ILKSYFQDIVDVDFTAQMEEQLDEVERGKIPWKKVIREFYPAFYETLKIAEEEIGDIN
                     LDEVTDVICENCGKNMLIKYGRYGKFLACPNFPECRNTKPIYEEAGVSCPLCGHKIII
                     KKTKKGRKYYGCEKNPECEFMSWNKPTGEKCPKCGSYMVEKGKKKKTAICSNEACLYS
                     KEIE"
     gene            19395..20171
                     /gene="codY"
                     /locus_tag="PLG49_06390"
     CDS             19395..20171
                     /gene="codY"
                     /locus_tag="PLG49_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOQ16989.1"
                     /translation="MSVELLEKTRKLNNLLQRSGVEPVIFNDICKVLSEVLESNTLII
                     SKKGKILGADYSNSSNKLFDADKGEVGSNVDTSFNDYLLRIIETQDNISLSDFPMSDE
                     KKLPANLIGTVIPIGAGGERLGTLLIYKDSGEYSTDDLILGEYGATVVGLEILRSKTE
                     ESAEELRKTAIVNSAIATLSYSELEAILHIFEELDGNEGLLVASKIADRVGITRSVIV
                     NALRKFESAGVIESRSLGMKGTYIKVLNDALIDELNKLRH"
BASE COUNT         7208 a         3014 c         3544 g         6696 t
ORIGIN      
        1 aactccgggc ctcttttatc tgtcatttaa gtatggagat aaagaagaat ggaatgaggg
       61 aaggtatttt agatatcata atgaaaagtc tttcgaaaaa attataagta aacataaaga
      121 attcgagatt cttaagatga gtaaatcaaa agacagagtt aagattcgaa aagataaaga
      181 aggtgaatac tggcttaatg tattacttaa aaagcggcat gattaaaaaa tattaaatca
      241 ttatttaaac tgcattcaaa aagctgtatg atatttattg tacagttaaa aaaattcagt
      301 tcattgttat tatacatttg tcaggacggc ttatatgaat aaataaaaat ctaaatcagc
      361 taagaaaaat taatgacata aatagaaagg aatgatttta tgccttttat aagtacgaga
      421 acgagcgtat cgattgatgc tgaaaaaaga gaaaatatta aagcaaaact tggtaaagcc
      481 atagagctta ttcctggtaa aactgaaaat tggttaatgc tttctttcca ggataatctg
      541 cctatgtatt tcaaaggtag caaccaggaa cctttagctt ttgtggaagt gatgcttttt
      601 ggttcagctt cgcaggaggc ctatgataag ttgacgaagg cagttacaga tattttaaat
      661 gaagaattgg gaattaaacc aaatggtatt tatgtaaaat atgaagaatg taaacattgg
      721 ggctggaatg gttccaattt ttaaaacgag gagttgttgc ctcgttttaa tttttggtcc
      781 ccaattatgt tcatactttg attttttata agcgtatagt tgtaaaaata tgaatagatg
      841 taaggaggtt ttgtttgaaa agaaataccc agcttagatt gcttgctttt ctattgccaa
      901 aaggctggat ataccaagag aaaataaaat aatattattg tttgaacttt tgctttcttt
      961 gatattttta aaagctaaaa aagctaaatt tccaaaaatt ttgggcgtca gcttttctaa
     1021 tatactgcaa atgctaaata cgataaaagg cgttaataaa atttctgcaa atattgataa
     1081 aataacgttt aaaaggctga tttcgaataa ataggctttg ggacaaaaat acgagctcat
     1141 ccagataaaa agtaataaaa agcatcctat tattaaatga gaaattttct tttctttgtt
     1201 tttttctaat aagttaaaaa tgatattttt aattggtatt ttggttgttt ggataatggg
     1261 tattatcgaa ctggctgccg ataaaaataa ggctaaaatg aaagttaaaa tcatatgaaa
     1321 tacagagata tctaattgaa tgcctccctg ccggacccag tcgggagccg ttccataaac
     1381 caatatataa gaaaataata aacccaaaag aagtcccaga aggccaccaa tgattccata
     1441 cacaagactt tccatgatta atacaaagga ggtatatttt tttgtggctc caatacttct
     1501 aaatgtacca ataataggta atctttccag tacaatcact tgaaatgagg aataaataat
     1561 taaaatactt acagataaca ccaaagcagt tattaattta aacgagactt caattcctcg
     1621 aatttcttgt tttatatctt ccagtgtata gggttctctg acatcaaaat cataatattt
     1681 gtctgaaagc caatggaggg ttgaaatgat ttcatcaggg tcttttaatt taaggtataa
     1741 aatattggat tggcctaagg caccattgat tttttccatg caatccctag ggcaaatggc
     1801 ataaatcata ccaatttcct gaaaaaagaa accatctcca tgggcaatgc ctgcaatggt
     1861 aaagtaataa tgagagttat tgattttcaa ttttatggaa tctcccaggg ataaatgata
     1921 tttttgagtg gttttttcac ttatgatgac ttttttccca ctaaaaggat agagattttt
     1981 ggattcagca aggcttatag gatttaattt ttccagatcc tccaattcaa ttccgagcat
     2041 ggatatagaa agtttagact gattgttgtc ttcgtatatg gctatactat taatgccagg
     2101 aatgatatat tcaaagtggt ctttatattg ctttaacagg tttgttgaga aaaaataatt
     2161 atctgctctt aaatttttgg aaaagtaaat gtctgctgtc cccatttgtg cttttatttg
     2221 ctttcttctt acttcaacca tattttttga atttgtaatg gaagccagaa aaagagcaga
     2281 agagagagta atcgataata tgattagaaa agtcctgaat tttttttctt taatgttttt
     2341 aagtatggat tttataataa tcacagttct cacctgcctg ttattctgtt tatgagtata
     2401 aaaataatta atcaaaaaat cataatgaaa atgttaaaaa aaaaataaaa aagaggtgtt
     2461 ttacctcttt tttattcctg aacaattggt agttcaaata tgactgtggt cccttttcca
     2521 ggactgcttt ttacagaaat attaccctca tgtccttcga tgattttttt acaaatcggc
     2581 aggcctaagc cgatgcctcc atatgcttca tttctggatt tttcagcccg atagaaataa
     2641 tcaaatatat aaggtaagtc ttcttgggga atacctgttc cgtcatcttg tatggaaact
     2701 ataaaaaatg aatcttttaa ttctgtttca atacggataa gtcctttggt ctttgtatat
     2761 tttatagcat tattaataag gttatcaatg acttgcttaa ttctttgagg atcaatcatc
     2821 aacttaacgg atggaatcat ttttggaact tcaagcattc tgttttgatt ttctgttttg
     2881 attataactg cttcaagtaa atctttaaag aattcatcag ccatccattc ttctttttca
     2941 atcgatatct tgtcggtttc cagttgggaa aaggtaaata aatcattaat taatcggtcc
     3001 aggctattta tctcattatg aataacctga agatatctgt taaacatctc ctcatttttt
     3061 gcgatgccat cctgtaaccc ttcaatatat cctttcaatg ttgtaatggg cgtttttaaa
     3121 tcgtgggaaa tgcaagcaat taattttttt cttgaatttt cataaaaaat ttgtttatta
     3181 agggattctt ttaatttaaa gcgcatttct tcgaaagtcc tgcaaaattc ccctaattca
     3241 tcgttacgtt tataactgac tttaaaatcc aggttgcctt ctgcgatatt ttgggttgcc
     3301 ctatttaatt cttttaaagg aataaggata cttcttgaca ggtaaaccgt aaacagcaga
     3361 atgagcacaa tcagtaatac aggcgtagta atagctgcaa tctgataata aagattgatt
     3421 ttgtactgaa ttccattgcc aaaatgacgc atatcttttg tgatgacagc actggcaacc
     3481 agttcattgt tgtaaaatat cgggctgtta tgacgatgaa agcctggatg ttgtaaatga
     3541 taattggggt catatccaaa gctgttttct atatagactt ttttcttttc cagggaagtt
     3601 tttttatctt ccgaatcaaa tagcagaaag ccgtttttat caatgacttg taacctgcct
     3661 tctactttgc tgagataagg ataaagcttt tcataaaact tgtcataatc atcaatcatc
     3721 aatcatgtca tagttctcct gaacttttgc gaaagtatca tataataact gattgatgtt
     3781 gtttaagtct ttaacatcat tattaaagtt atagagcagg gaaaaaggaa taaagacaca
     3841 taaggaagga ataagaataa cggctaaaaa ggccataaat attttgcttt gaatttttaa
     3901 tggctttttc atgattgtcc cccttcaaat ttataaccta ctccccaaac ggtttgaatg
     3961 aattctgcag cctgaggatt cttttctaat ttttttctga tattccggat ataaacggtt
     4021 actgtatgta aatctgcata atcattgtaa ccccaaactt tatccaaaat ttgttccctg
     4081 gtaaaaacct gattaggatg aagggcaagt aattttaata tttcaaactc tttagtagac
     4141 agattcagtt tttcatcttc taaataaacc gtataagact tcagattaat tttaagggga
     4201 gggtatacca gttggtcttg attatgatga gcttcagaaa actggagata tcttcttaat
     4261 tgtgctttaa ctctggcaac caactccgag ggactaaatg gttttgtgac ataatcatct
     4321 gcacccaggc ctaaaccaag tattttgtct atatcgcttt tttttgcact taacatgata
     4381 atgggaacag aagaatgaga acggataatt ctgcaaactt caagaccatc aatgacagga
     4441 agcataatat ccaaaatcat taaatcaggt ttaaattgtt gaaacagttc caaagcttct
     4501 tgtccattat aagccagttc aacttcatac tcttcatttt ctaaataatc ccggattaat
     4561 tcagcaattt cttttttatc atctacaatc aggatttttt ctccagccat tttaaattcc
     4621 actccataaa ataattttat gatatatttt aacttatcat atttttcgat atttttctat
     4681 taaatcatta aaatagttca aactttaata aacatatgaa aaatatttaa cctgtttgat
     4741 aagatttttt taatgaacat agattagaag agtgagcata aaacttttaa ttatatattt
     4801 tgataaagag agtagaagga atttatttag ccagttattt acttgttgac aggaaagtat
     4861 aattttaaag ggagatctaa acttatccag atcagagatt aaccttttgg cggagccgtt
     4921 aaaagtggct ggaagcctag ttctttcggg atgcaaaaat atcagactat tacccagtca
     4981 gattttagat tttctatagc taattatgac ggatggtaag atagcagaaa actcggataa
     5041 aagaagtgga aaatatgccc gaataaaaaa agccagttga ctggcttttt ttattcaatg
     5101 tctttaatga aaagctcatt tttattaatc attttatcac agtggggaca ataatatttt
     5161 agttgaattt tgtgatgaca ggactgatga atgacttctt tatagcattt ttttaaatgt
     5221 ctttctcccc aaaggataat gctgtaaaat atatcttcta aatcttttcc gctttcagtc
     5281 agttcatatc gatagcgggg agggtgtttt tgatatagaa caggaatgat tagttcatct
     5341 ttttctaaag cctttagacg attggataag aggttggtag aaataccttc taagttttca
     5401 agcaaagcat taaaagtatc atttccttta aagatttgac aaagaattaa taaggtccat
     5461 ttatctccta tgatatttag tgtttgtgca atattgcatg gtaaattata ttgatttttc
     5521 atatccatac ccctttaaat ttatctttat cgtataaaag acaggattat ggtattgtaa
     5581 taatggcatg aaaagtattc ttaaactgga tttagtctac aggatttctc aacagttcta
     5641 tggtgctttt catgacatct ttagtaatca ttcctggtgc atagccataa atattgcctt
     5701 ctttatcaat aaagtaactg gtcggcaagg aataaatctc atagtcataa aaaacagagc
     5761 cagtttcatc aaataagata gggaaagtaa tatgcctttc atctaaaaat tgaataaccc
     5821 cttctttgtc tacttccttg gtataaggat tttcagcagt tttagggcta gaaataccta
     5881 aaataatgac gtcttcctga ttttctccat attcatgata cagttcttcg atgtcaggca
     5941 tttcagcccg gcaaggtgga caccaggttg cccaaaaatt caggaatacc actttgcctt
     6001 tgtattcaga aagcgtatgc tcattgccat attgatcgac caaagtaaaa tcaatggcag
     6061 gatatattgt ttcttcattt gtttcttgat tttcttcatt tgtttcttga ttttcttcat
     6121 ttttttcatc ggttgaagaa tctgagattt cttcttgctg attgggcgtc ctggaactac
     6181 caaaagaatt taagtagctt gatatactat tcatccagcc ggtaaacagt ataattccga
     6241 taagaattaa aagcactccg ccaattttta cagtgtattt tacaatattt ctatgtttat
     6301 ctaaaaagtt tatcacttga gtcgtaaata agcccagtaa taagaatgga atgataaagc
     6361 caagagtata taatagaact aataaattac caataatgta gctgttggaa cttgaggcaa
     6421 aaatcaatac agaagacagg gctggaccaa cacaaggcgt ccaggcaaag ctaaaagtaa
     6481 atccaagaag aaaagcgaca aaaggattca cttttttatt atcgatattc agatgaattt
     6541 ttcgttcttg atttaaaaat tttatattaa ataaacctaa ctggaataaa cctaaaataa
     6601 taacaagtat tcctgcaata cgggtaaaaa gtaagcggtt actgtttaaa aaacttccta
     6661 cagctgtaaa agtcataccc agaataaaga atgcaaagga aattcccaat acaaaaaata
     6721 ttgtattgat aaagactttt tttctatcaa aaatcatttt accattttca gaagtctcat
     6781 tggagcttcc tgccaaataa ctcatataaa gcgggattaa aggaattacg caaggtgaaa
     6841 aaaaagatac aataccttca ataaacacca gaaaaaaact aatattacca gctgaaacga
     6901 tggaatccat tttctcactc cttatatttt ttttaattat atcatgggta attataattt
     6961 atcaagttaa ttcaaaaaat gaagttacat tcagaaaaat taagggaaat atttaaatca
     7021 attttttggt gaagtccatg gtggtgtgta tcctttaaat gatacagccg gatagcagaa
     7081 atatcaatta tcaaagattg actatcaggt aatgcataaa atgctttcat gcttgccaaa
     7141 ctatattgac taaattttta tcataggctg ctaaaggagt attggtaatg gatattttta
     7201 tcaaacacca attagataaa gacgcgtctg gttatatttt gactttatac atcaatccgg
     7261 atttaactga gtttgcaaaa gaattaacta ataaagaaga tatttcaaca aacaaaaatt
     7321 tttactacag tataaaaaac tacatcagtg aaaattttaa aaatattaaa atcaacacca
     7381 ttaagcttat ggctggaagc atgctgattg ccagcatccc ttttacaagt cctgtttcag
     7441 tcagtgctgc agcaacagga gttcaaagca gccaaagttc aagccctatt tctattatca
     7501 ttaacggtca aaaatatcag ttttcccgtt ctcctgtgat aaaaaacggg acaacttaca
     7561 ttcccctaag agatgttgcc gagactttgg gggcagaagt atggtggaat gcttcgtcta
     7621 aaacagtagg tataaataaa gataatacta aaatttcttt tgttgtgggt tcaaataagg
     7681 tccgggttaa tgaccgttta attgaaatgc agcctgctta tatagacaat ggaacgacta
     7741 tggttccatt aagtttatta gtgaaagtct tggaatggat gttcactggg atcaaaatac
     7801 aaaaacaatt aacatccgtt catcccttaa atcgcataaa gtaaaagcag gagaaagttt
     7861 atggaaaatt gcaaatcaat acaatacaac agtcagtaaa ataaaagaat taaatgctct
     7921 gaaaggagat accatttatg ccggtcaaga gctaaaaatc gaaacagata cgaatgcaaa
     7981 aatcaataca ccgtcaagca gtaattatat aacttatatg agctacagcc ttaaagcagg
     8041 tgataatttg tgggatctga gcataaaata cggagtacct ttcacagaat tactggctgt
     8101 caataatatg acagaaaata gccccttatc tgttgggcaa aaaattaaaa ttcctgttca
     8161 tcatattgct cagaaagaaa gggtcagtga acgtcatgga gagtatttag attggtggac
     8221 agaggctcaa tatctgtttc ccatagataa agtggccacc gtaacggact ttcaaacggg
     8281 aaagagtttt aaggtaaaaa ggaccacagg ggcttttcat gcagattgcg aacctttgtc
     8341 tgcaaaagat gcagccatta taaaagaaat atggggcggg aattattcat ggaaagaaag
     8401 agcggttatt gttcaggtgg acggaagaaa aatagctgct tccatggctt caatgccaca
     8461 tgatgtagcc tgtattacgg ataataattt taatgggcat tttgatattc actttaaaaa
     8521 tagtattcgt cataaagatg gtttaatttc aacggctcat caggaacaag tgaaaattgc
     8581 tgcaggaatc cggtaaatat taaaaaaaca tcttcaaaaa tttgaagatg tttttttaat
     8641 ctagaatcgc aaatctcttt ccccgttacg gattctgttt aaatattcaa gggttctttg
     8701 tctgcgtctt tcgctaggaa tgttattgat ttctttgtcg attaagcttt gtcccatttc
     8761 cattaattca tcatcggcat aatctcttaa atattcttcg aaagtcaaaa gggcattagg
     8821 caaacagcag ttggatattt ggccggattt ggcaagaggc ataaagcgat caccagttct
     8881 gccttcccta tagcaggctg tacagtaact tgggatatat ccgtctttaa caagaccttt
     8941 tatgacatca atgggttttc tatggtcggc taaataaaat tggggttttt cttctacacc
     9001 atcttctata taaacgtctt ttttagcata tcctccaacg ccaactgaag aacctgcact
     9061 catctgagat attcctactg ttaacaattc gttacggaat tcggcagatt ctctggtgga
     9121 aagaatcata ccagtataag gcacagcaag acgaatgact gcaaccactt ttttgaaatc
     9181 tctgttattg attaaataag gaaattcttc ataattgaca ccttcagccg gcagcaatct
     9241 gggtatagaa atcgtgtgag gaccaatacc gcaaacggct tctaaatgtt ctacatgcat
     9301 aagcaatgca atggtttcat aatgataatc atataaacca taaaggacac ctataccgac
     9361 atcatcaatg cctccgccaa gcattgccct gtccatagct tctgtatgcc attcataatt
     9421 tcgtttgggt cctttgtgaa cttcaagata agtgggtttg tgataagttt cctggaaaag
     9481 ggtatatgtt cctattcctg cttcttttaa ttttttatag ttttcctcag tagtggctgc
     9541 aatgtcaata tttactcgtc ggatagaacc atttttaaat ttctcattat aaatggtctt
     9601 tatgcattcc aaaatataat caataggaca gttctgatca tcttctcccg cttcaagaag
     9661 tagtctttta tgtcccatag cttctaaagc ccttacttct tccctcaatt cttcctgggt
     9721 aagtttatgt cttgcaattt gattgctgca ttttaaacca cagtatttac aattatttac
     9781 acagtaattg gataaataca ggggggcaaa aaggacaatt cgtttaccat atattttttc
     9841 tttaatatca tttgctattt tataaatttt gtttaaagtt tcatcatcgt ccacttctaa
     9901 taaaacagca gcttctttgt gagaaagtcc ttttaagttt gcagctttat ctaagatcct
     9961 ttcaatttca tacttattct gaacggcttt ctttgcatct tccattgaat ttaatatctc
    10021 ttcatggcat ataaattcat tggaatcaaa agattttaca ttgtacataa tataactcct
    10081 tccaaatcag ctgattcatt atttttttgc agatagtttt gaataaacgg tttttgtgct
    10141 gacatcagga agcataccaa gttttcctga aagggcacta atcacatcat tgggagcatc
    10201 catcacaata ctaataattg aaatatttcg tttgtgataa ggaatgccca ttctgccaat
    10261 aataaattca ttataatcgt gcaggatttt gttaagtttt tccactgaat ccggattctc
    10321 aacaatgata cctacgatag caattctggt ttccatatca tctccccttg ttaaaaaatt
    10381 aatttgctag tttacaataa ttctggattt ttgcatagaa aaatccttgt actaaaaggc
    10441 acaaggataa taacaaataa atatgcatta ttattctctt gccttcacaa tcgggacgaa
    10501 cccttttgta tcagtacaat cgaattatta atttatttaa ttatatttat tatattgtat
    10561 cgacaaaatc ttttcaagct atatgttaaa tccaaagcaa tgttgtataa tatacgatag
    10621 cagaactatt tatactattt cgaaagaaca ttctgttatg aaaaatatat acaaagaggt
    10681 gactattgtg aacatacttg ttacagtaga ctcgaattat attctgcctt taaaagttat
    10741 gttaaaatca ctttttttaa ataatgcgga agatgaattt accatttatc tcatttattc
    10801 tagaatcaaa gaggatgaat tagcagattt aaacacttat ataaagcagc atggaagaca
    10861 attaaaagcc attaagattg ctgatgatta ttttgaggat gcaccgacac tgatgcatta
    10921 tacaaaagaa atgtattaca ggctcctggc ttttaaattt ttacctgaag aacttgataa
    10981 aatcttgtat ctagatccgg atatcttagt catcaataag atagatggtt tatatcattt
    11041 agatcttgaa gactatttat ttgctgccgc ttatcataat aagatttctg tcagggaaat
    11101 taaccgcttt agactgagtc cgtatgaaat agataaatat tataattcgg gtgtacttct
    11161 tatgaattta aaactgcaaa ggaatttaat gaatgaaaat gagatatttg attttgtaag
    11221 aaaacataaa tctaaattaa tcatgccgga ccaggatatc ttaaatgcct tatattcaaa
    11281 gaaaataaag agtattgatg agattttgta taattacgat gccagattct ataattatta
    11341 taagatttta agcagtggtg aatatgatat ggattatgtc atttatcata cggttatcct
    11401 gcatttttgt ggaaaaaaga aaccctggca aaaaaaatat agtggcaaat ttcattcatt
    11461 atataagcat tatgaaaaat tagctttatc ctaaattccg ctaccagata tataaaattt
    11521 gaggtcttca gatgaccttt gaattttttt aactggtact cttatttttt tgtaacgaaa
    11581 ttttcttaat ccgtacataa tagttgagga ggtgaggcaa tgaaatccat caacaagtac
    11641 agaggagaat gtaagcctgc cgtctggtta tgttaaattg ccagatttgt gcggtataag
    11701 tatttagaaa agaataaaaa cacaaagcaa tttctcttga ggatatagaa atagaagatt
    11761 cggataacaa tatcgaagaa tttattgatc gtcaggataa aatcaatttt tataaaaaaa
    11821 tcaaagtctc gataaagatt accgggatgt gatttatttg agattattgg gagaattatc
    11881 ttttgctgaa ataggtgaga taatgaataa atcagaaagc tgggccaggg taaccttttt
    11941 tagggggaaa cagcaattaa tgaaaggaga gatttaatga gtttaaaatg ccacatcgta
    12001 aaagatttat tacctaatta catagatcat gtgacttctc aggaaacaaa tcaagacata
    12061 caagaacact taaaggattg tgaagacagc aaaaagatct atgatgaaat gaattataaa
    12121 attactgatc aattaattga tctgaatcag gttaataata atcttaaaga aatcaattat
    12181 ttaaaaaaat acaaaaatac aaaaatactg ttcttcgtat tatcatactc ttgtttgtta
    12241 ttattggaat atatacagtg ctttttgttg tcattgggta tttgtggatg tatgatagta
    12301 aggaaacgat taatgatata aggatgtatg aagaaatttt aggacacgat ggaaaataca
    12361 agtatcattt taatcgttat aaagatattt tctctgatga aataacagat tcaatgaaag
    12421 ttgaaaaatt ctgctacatt tactataatc cctgggatgc ttcttatctt ggatatttgg
    12481 tttatacctg tgatgacgaa agttaccgaa aggaatacga acgcttaaaa aatataaagt
    12541 cctcagataa ttatttaatt tacggggcaa aagaatttcc ctatgaatta tatgcggttt
    12601 atgcagatcc gtattatgga tatatttatg ccatgaccaa tcctgaacgc aatcaattta
    12661 tatatgttga gcttcaattt tataactatt tttcagaaat tgataatgaa aagttcatag
    12721 ataaagaaca tcttccatta ggttttgatg caaaatggca taataaaata caaaaagaat
    12781 ttttagaagg ttcttggtaa acaactcaaa aataatatat tcgagaagag gagattaact
    12841 tatgaaaatt gaaaaagtct tttcaaggca tttaacagat gacgggaaat tcatcagaaa
    12901 aaaagtattt attgaagaac agggtttcaa agatgaattt gatgaatatg attgtgatga
    12961 aaaagccatt catcttgttc tgtatgataa tcagcaagcg gttgcaacag gcagattatt
    13021 tacaaaggat tctcaaaaga aaacttatac tattggcaga ttggcagtta taaaagaata
    13081 tcgtaaactt ggactgggaa aagtactggt gcagtctctt gaagaaaagg caaaagaaga
    13141 aggggcagag aaaataacca tatcggctca atgcagagcg gaaggatttt ataaaaaact
    13201 gggttatact tcatcgggag acatttatta tgaagaaact tgtcctcata ttcatatgga
    13261 gaagatatta tgaaggttcg taagattgca gccaatgctg tggctttttc ttatgagaat
    13321 attgaagatt ttgtaaccaa tgtttttctt gtcgaaaccc cttcaaaatt ctttgtgata
    13381 gatacttatt gcggttcaga tgctatgaaa cccgttttag aacgaattaa ccattcttca
    13441 aaaaataaag aaataattgt gattaacagc cattttcact gggatcatgt atggggcaat
    13501 gccagcttta tcggaaagga tattataagc cacgaaatat gcagaaagtt tcttgatgag
    13561 caatgggaaa agcaaatgaa gcataacagg caatatatct taggcagggc agagaagcaa
    13621 ttacctaaca tcacctttaa ggaaaaaata attttttacg atgaaggaat tgagcttttt
    13681 tacagtccgg gccatacgat agacagtatt tccttatttg accataatga aaaggtatta
    13741 tatgcaggtg ataatttaga aaaaccaatt atttatgttg aaaataatga tattaaaaca
    13801 tatattaaaa gcttaaaaaa ttatttgaat tacaagccaa aaaaaatcgt tgcaggacat
    13861 agcctggatt taaaagaaga agatattttt agcgctattg agtatctgaa gggtctttct
    13921 gaaggccgga aatttacttt taaaacagac tatgaaagaa aaattcatta ccaaaatatc
    13981 agtatgataa ataaaaagta gactcatttt tattttattt tacacccatt tttattaatt
    14041 tttcataaac ttttggaagc tttttcggtg ttaacgtagc tgcttctttg tttaatacag
    14101 taacatctaa acctaatttt atgcccagta ttttctgtct aaacttcgat attgtatagc
    14161 ctcgctaaga tgctggagtt caatattttc tttttcatct aaatcggcaa ttgttcttgc
    14221 tacttttaag attctatgat aagctctggc gcttaaattc aatttttcaa aggcagttct
    14281 taataactct ctttcttctt ttcgcaaaat acaatatttc tcaatttgag cagcgcctag
    14341 ttgtgcatta aaataaatat tttcattttt atagcgctct tgttgtattt tttgagcttt
    14401 taagactctt tttttgatgc tggctgagga ttcacttttt tcttttgtgt ttaagtcttt
    14461 gtacttgaca ggcgacactt caacatggat gtccagcctg tcaagaaggg gacccgatat
    14521 ttttgcaaga tattttttga ttgtttcaga ctggcaatgg catttttcct cgtccccata
    14581 gtaaccacat ggacatgggt tcatcgagca aaccagcata aaactggctg gataagttaa
    14641 agtagcgtta actctcgata tagttacttc tctgtcttct aaaggctggc gcagtacttc
    14701 taatacattt ttattaaatt ctggaagttc atccaaaaac aagacaccat aatgggacaa
    14761 acttacttct cctggtcttg ggattctccc acctccaact aaggcagaag ttgacacagt
    14821 atgatgaggc gaacggaaag gtctgtttgt aattaaagat tttttgtccg gaagcaagcc
    14881 ggaaacacta tatattttag ttatttctat actttcctca aaagttaagt caggaagaat
    14941 ggtaggaagt ctttttgcca tcatagtttt gccagatcct ggtggtccaa tcatcagcaa
    15001 attatgagag cctgcagcag atatttctaa tgccctttta acatgtgcct gtcctttgac
    15061 atcagaaaaa tcaagttttg agttcgaaga ttgttgcatc agtgtctgaa tatttacagt
    15121 ggtttcagga atggattttg ttcctaaaag atattctaaa gcttccatta agttatgtac
    15181 gccaataacc ttaattcctt caacgattgc cccttcttca gcattttcaa aaggaacaat
    15241 gcattgttcc agtccgtttt tataggcaga ataaaccatt ggcaagatac cattaacagg
    15301 tttcacgctg ccatctaaag acaactcccc aacaatcata gttttttcaa gtttttccgg
    15361 gcttattgaa agattacaac taacaattcc gacagcaata ggaagatcaa aggcagatcc
    15421 ttctttttta gtatcagctg gggcaaggtt aattgtaatt cttttaacag gaaataaaag
    15481 ctgagaattt tttattgcag cccggacccg gtcttttgct tcccgaacag aagaatcggg
    15541 aagtcccact aaatcaaatc cgggaagtcc ttcactcaaa tcaacttcga catctaccag
    15601 atacccgtta ataccatata aagagcaact tttaacttta ctaagcattg tgttcttcct
    15661 ttcattaaaa catgaattta ttaatattat tccacattat tacattaata aaccattatt
    15721 ccacattatt acattaataa accaacagat gagaggcttg aattaattca aataagtggt
    15781 aaaatataaa aagttataca gagcgggtgt gttataatca tgtaataagt aagatttaat
    15841 gataacagaa ggtgattttg tggaagggct tttagagagt acgatttatt ggttttggat
    15901 aagcaattta aaaggtctta aaaaaagtag aatccagcag ctattaaagc attttaaaac
    15961 accaaaaaat atttggaagc tgggaaaaaa tgaaataaaa agtgtgataa atatttctga
    16021 agaagaatta aatatattgg aaagatcaaa aaattatgaa gggttaatag aagctttatt
    16081 cgatttaaaa gaaaaaaata tcttttttta ttctattcag gatgaagctt atcctgtgat
    16141 gctgagaaac attcctaatc ctccgccagg aatttatgta aaaggtcagt tacctgcctt
    16201 tgaaaaagga tatatagcca ttgtgggatc tagaaactgc actgaatatg gtaaaaatgt
    16261 tgtaagggaa ttgggcaggg aattggttaa aaggggaatt ggcatcatta gtggtttagc
    16321 ttatgggatt gacagtgagg cgcataaggt tgctttagaa tttggaggga atacggttgc
    16381 agttcttgca aatggtttag ataactgtta ccctcctggt aatataaatt tgatgaaatc
    16441 tattgaagta acaggagcac ttatttctga ataccctccg actacaaaag caattccttc
    16501 tttcttcccg gccagaaacc gaattattag tggtcttagc aaaggcgttc ttattgtaga
    16561 agcaggagaa aaaagcgggg ctcttattac tgcagatttg gccttagaac aaggaaaaga
    16621 agtgtttgct gtaccaggaa gtatctttag cagtacaagt acaggaacca ataaacttat
    16681 tcagcaggga gcaaaattag ttacaaaagt tgaagatatt ttggaagaaa taaaattaaa
    16741 tatggatatt tataataaat ataataataa agaagataag ccatataaaa atgaaagtat
    16801 aagtggactt gcattagaag aaaatatagt atatgattgt ataagttttg aagccacatc
    16861 aatggaatat ttatctgtta aaacaaagta taatatcgat aaattgcaag caatacttac
    16921 gttattggag ataaaaggat taatcaaaca gttgccgggt aagaagttta tccgaattta
    16981 atgatggagg ggtaatatgg caagctatct ggttatagta gaatcaccag ctaaagcaaa
    17041 aacaattaaa aaatttttag ggaaatcata taaggtagaa gcgacgatgg gtcatattag
    17101 agatttgccc aaaagtcaat taggcgtgaa tatagaggaa gactttgaac ctaaatatat
    17161 taccattcgt gggaaaggag aaattttaag caagttaaga aaaattgtta aaactgcaga
    17221 taaaatatat ctcgcgacgg acccggacag agaaggagaa gctatatcct ggcatttaat
    17281 gcatgccctt aatctcgatg aaacgaaaac caaaagaatt acttttaacg aaattacaaa
    17341 aaatgctgta aaaaactcca taaaagaggc cagagatatt gatatgaatt tagtagatgc
    17401 ccaacaggca agaagagtat tagacagaat tgtcggctat aaaatcagtc cgcttttatg
    17461 gaaaaaaatc agaaaagggc ttagtgccgg tagagttcag tcggttgctt taagattgat
    17521 atgtgacaga gaagaagaaa ttaataattt tgttccagag gaatactgga ctatagaagc
    17581 aaaattcctg gacaatgaaa aaagagcaat tgagtcaaaa ttttatggtt taaaagacaa
    17641 taaaatcgaa attgattcaa aagaaaaagc ggataagatt cttagcgaaa tcaagaagca
    17701 aaaatttaaa atagaagaag ttaaatttgg agagcgtatc aaaaagcctg tagcaccttt
    17761 tacaaccagt actcttcaac aggaagcttc aaaatattta gggttttcaa ccactaaaac
    17821 aatgagaatt gctcagcagt tatatgaagg tgtggatatt gaaggagaag gtactgttgg
    17881 cttaattact tatatccgta cggattctgt cagaatttca gaagaagcta aagctcaggc
    17941 aaaaaaatat atagaaactc attttggaaa agaatatatc aatacaagag aatatagtta
    18001 taagaataaa gggaaaagtc aggatgccca tgaagccatc agacctactt atattgataa
    18061 agaacctaaa agtataaaaa attctttaag taatgatcaa tataagcttt atgaattaat
    18121 atggcaaaga tttatggcca gtcaaatggc acctgccgca tatgatactt tttcaattaa
    18181 aataagtgct ggagattata tttttaccag ttcaggttca tctgttaaat ttgatggttt
    18241 tttgatcgta tataataagg aaaaagaaaa agaagaagaa atgaatttca gcacaaatat
    18301 tgatgaggaa ttaaaattag accatttaga agcaaagcaa cattttactc aaccacctgc
    18361 cagatattct gaggcaacat tagtcaaaac tttagaagaa aatggagtag gtcgtccaag
    18421 tacgtatgcg cctactatca gcaccctttt taacagaggt tatgccgtaa aagaaaataa
    18481 ggtcatttat ccgacggaat taggggagat tgtcatcgat attcttaaat cctactttca
    18541 agatatagtt gatgtagatt ttacggctca aatggaagaa caattagacg aagttgaaag
    18601 aggaaaaatt ccctggaaaa aagtaatcag agaattttat ccagcttttt atgaaacctt
    18661 gaaaattgct gaagaagaaa ttggggatat taatttagat gaagttactg atgtgatttg
    18721 tgaaaactgt gggaagaaca tgttaattaa atacgggcgt tacggtaagt ttcttgcatg
    18781 tcctaatttt cctgagtgca gaaataccaa gcccatatat gaagaagcag gtgtttcttg
    18841 tcctttgtgt ggtcataaaa taatcattaa aaaaacaaag aaaggcagaa aatattacgg
    18901 ctgtgaaaag aatccagaat gcgaatttat gtcatggaat aaaccaactg gcgaaaaatg
    18961 tcctaaatgc ggcagttata tggtagagaa agggaaaaag aaaaagacag cgatatgcag
    19021 taatgaggcg tgtttatata gtaaagaaat agaataatat atttataaaa gctatctaag
    19081 agaaatcgac aaaaaaagac aactttttag catagttttt taaaaactat tgcttttttt
    19141 aattctaaat gatattatta attcggcaaa tgattaaaaa attaaacttt ttagtaagat
    19201 tctttaaaaa tattgttttt ttaaataatt agtgctatga taataatata aatttatgaa
    19261 ttaactaatt attagtaaaa aaaacaatta caacaataat tattataaaa aaattatcgt
    19321 ttttatagta taaagtatga atttttagaa cataatacta gacagaaaat aaagattaaa
    19381 caaggaggaa taaaatgtca gtagaattat tagaaaaaac gagaaagttg aacaatttgt
    19441 tgcagcgttc tggagttgag ccagtaattt ttaacgatat atgtaaagtg ctgagtgaag
    19501 tcttagaatc caacacacta atcataagta agaaaggaaa aattttaggg gcggattatt
    19561 cgaattcttc taacaaattg tttgatgcag acaaagggga agtaggcagc aatgttgata
    19621 ctagttttaa tgattattta ttaagaatta ttgaaactca ggacaatata tctttaagcg
    19681 attttcctat gtctgatgag aaaaaacttc ctgctaattt aataggtact gttataccaa
    19741 taggtgctgg cggggaaaga ttaggtactc tcttaatata taaagacagt ggagagtata
    19801 gtacagatga tttaatactt ggtgaatatg gtgcaactgt agtaggtttg gaaattcttc
    19861 gttccaagac tgaagaatct gctgaggaac ttagaaaaac cgctattgtt aattcagcta
    19921 ttgctacttt atcttattca gaattagaag ctattcttca tattttcgaa gaattagatg
    19981 gtaatgaagg tttgctggta gcaagtaaaa ttgctgacag agttggaatt accagatctg
    20041 ttattgtgaa tgctcttaga aaattcgaaa gtgccggagt aatagaatct cgttctttag
    20101 gtatgaaagg cacttacatt aaagtattaa atgacgcgct aatcgatgaa ttaaataaat
    20161 taagacatta aaaaagtggc ttgccacttt ttttgtgtat tttagctatt aatcaattaa
    20221 cataatacta aattatagaa attattaaca atattaatta ttatattatt tttattgatt
    20281 taaattgata ttaaaaaaat tataataata tgggaaaaaa ataagcaaat gggacaaatt
    20341 tctcaatatg attttgttgc tcttttatta tttaatgtat ataatgataa ttaaagtcga
    20401 ataattattt aattaatgga agattgaaaa aaattatatt aatgtgtggt tttttgttat
    20461 at
//
LOCUS       DAOSUD010000039        19473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1803_length_19473_cov_23.343650, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000039 DAOSUD010000000
VERSION     DAOSUD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_19473_cov_23.343650"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1065
                     /locus_tag="PLG49_06395"
     CDS             73..1065
                     /locus_tag="PLG49_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ16990.1"
                     /translation="MKKLTSLLAIILSLLTLVVGCGNQNTDSKNTDSGNVSIGIIQYM
                     DHVALDAAREGFIAALKDNGYVEGENLSIDYQNAQGDQSNLSTISDRFVSNKVDLVLA
                     IATPAAQSIAGKTTEIPILATAVTDYEAAKLVDSNEAPGGNVTGTTDMNPIKEQIELL
                     VKLVSDVKTIGLLYTSSEDNSIVQANIAKEVIESLGLSHVEVTVTNSNDVQQATQSIV
                     EQCDAIYIPTDNTFASAMPVVHGVTAESKTPVICGESGMVNNGGLATLGINYYDLGYQ
                     TGLMAVKVLKGEAEPATMPIESANKFDFAINGTVAEEIGIEIPDDLKEYVFEAE"
     gene            1117..2028
                     /locus_tag="PLG49_06400"
     CDS             1117..2028
                     /locus_tag="PLG49_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ16991.1"
                     /translation="MTQIILGAVSLGLLWAIMTIGVYITYRILDIADLTVEGSLPLGA
                     AIAASAISSGVNPYLATGVAIIGGLAAGLTTGILHTKLKIPALLSGILTMIALYSINL
                     RVMGKANVSLLRMDTVYTVFQKLGLNETNAVIILGIIVVFSLVCLLYWFFGTELGSAI
                     RATGNNPRMVRAQGINTNTMKILGLVISNGLIALSGALIAQSQSFADVQMGTGSIVIG
                     LASVIIGEVLFGKRSFFSRLISLVLGAITYRIIIAFVLKMGMPANDLKLFTAITVAIA
                     LALPNIKKSLQPTFSKFFRGGENNAVR"
     gene            2015..2809
                     /locus_tag="PLG49_06405"
     CDS             2015..2809
                     /locus_tag="PLG49_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ16992.1"
                     /translation="MLSVNNIYKVFNPGTVNEKVALRGISLTLEEGEFVTIIGGNGAG
                     KSTLLNAIAGVYPVDEGSIIIDGTDVTKLPEHKRAAFLGRVFQDPMMGTASNMWIEEN
                     LALAYRRGQRRTLRWGIKQKEREYYKELLKTLELGLEDRMTTKVGLLSGGQRQALTLL
                     MATLKKPKVLLLDEHTAALDPKTAQKVLEQTDKLVEENNLTTLMITHNMKDAIRHGNR
                     LIMMHDGKIILDIKGEEKKNLTVEDLLERFGKVSGEEFANDRALLS"
     gene            complement(3045..4091)
                     /locus_tag="PLG49_06410"
     CDS             complement(3045..4091)
                     /locus_tag="PLG49_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOQ16993.1"
                     /translation="MIIVPAVEGVNSYTLILPATLFKEGQDEKKVIFSTPEGEVIVPY
                     NIFLQTIEDEKSVNIYIARLEESALSPKIKELAGNNPVVQLTLETGNKKVNMTRPVMV
                     SIPYKPSAEINLDWEYITIWKMDHSGNETPVASAEYDPQERKVNFTTTFSGQYAVVWQ
                     HKTFKDIDRVAWAQKAIEFMASKGFITGLTDNLFVADASITRADFVTYLIRSLGLTAE
                     FDNNFADVLPESDYYETVGIARKLGIALGDENNKFYPDETISRQDMMTLTDRALRQYK
                     EFDYFYDHKILDKYKDKNEISDYAINSIAIMVGEGLIVGYDDRINPYGKASRAEAVVF
                     LYRVYNKYVLPVRP"
     gene            complement(4285..6126)
                     /locus_tag="PLG49_06415"
     CDS             complement(4285..6126)
                     /locus_tag="PLG49_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOQ16994.1"
                     /translation="MEESAFNGCKSLYSIFIPDSVTSLGEYVFYDCNNLEFIQFSSDS
                     QLREIGQKAFYGCTGLRSFVIPNNVTSIEDFAFYNCMNLTSISIPEGVKRIGQYAFYE
                     CDNLRTIVLPESLIDLDEYVFYGCDNLKEIKFLEPSGISAIGKNTFYYCTGLQSVIIP
                     EGVTSIDEFAFYGCTSLVSIILPESLQTIGESAFNACNNLSSIYIPQNVIDIGGYAFY
                     DCDTLERVEFADESKIKVIGANAFYNCKGLRTIIVPESVTDIEAYAFYNCENLTSITI
                     PWQVTSIGSGAFDSCSSLREVLMQPLIAPAIEENIFNNCASDLVVFVQPTAQGYFDGI
                     WANFNVHQIKLMSIVAPEPVTGIKNGAVKTVQGLGLPNTVTLILSGGIKRQASIIWKV
                     DECAYNPNDTKKQTFEVEGVVILPDDLPNPDGIPLLAKVLVTVEEAESGFLPVSAITV
                     IAAGGSDRVVVGNKLQMLAIVEPDDAGNKAVVWSIVKGNGADIDENGLLTAHSVGTVV
                     VRATAQDGSLVYGEKEIYIIDDSDIKEKWYTVIYDGNGHTGGTVPQDYNLYMAGDRVT
                     VLDNTGKLTKSGYVFIGWIYDHQFYLPGDTFIINKDVKLEAYWFPIR"
     gene            complement(6130..6519)
                     /locus_tag="PLG49_06420"
     CDS             complement(6130..6519)
                     /locus_tag="PLG49_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOQ16995.1"
                     /translation="MNSKIISKSLLSSFIMVFLLILFPLKPLGAVDSEFVDSESGLKL
                     RVITEEGDTGTVEVIRHDYTESFYNIPATIEYNGIVYTVTSIGEYAFYDCKSLTSVTI
                     PATVLAIRSHAFYLCDNLVSVVISEGV"
     gene            complement(6611..8857)
                     /locus_tag="PLG49_06425"
     CDS             complement(6611..8857)
                     /locus_tag="PLG49_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOQ16996.1"
                     /translation="MEKMKKLDIKIFIIPLLLITIIFIATGRMVFDSVREHYYHYLNQ
                     ESVKLAQGYSHSLAKASEAREVVNQLLEERLIVAGDAVVLYDGQYNNELFARLANILK
                     VDEIDYYEAGGKLIYSNLEELIGWEAYEGHPVYDFMKSNELSRIDHIRRDSITGNYYK
                     YGYFKVSNGGLVQIGVRADKVYSFLDRFNIQQLLDEMKVGEEISLIYFMDNDFNIIGS
                     SDTHMIGEKFKNEKVIPAILNNEVYSFVTEQNGKMIYEIFQPVYFEGKKIGTIAIVKS
                     LNNAETIIKQLSVLGVVILTVIYCLVLFIMFSIYKVSGRLFHLAYYDSLTGLLNKHYL
                     RELINEELKKKEDSKKAFLFINCRHFKLINLVYGYDYGDEILKALGERLKTVQDNNRI
                     LFRYSADRFVLFVKNYKKPEELISISKEITGLFNNSLVVQDIEQLLDIQVAMVEMDRY
                     RYKDIDRVLKDASITLSYIKSNDSKKYAFFNEAMEKKLQREDLIEKEIRSIILNEDDK
                     KMYLEFQPQFDLRSNKIVAFEALARMNIPTFGFVSSLEFINLAERKQLIVPLSNLILK
                     KACDFLKDLTDKGIDSVKVAVNISGIQLLRDDFISNIMEIIKERGIDPSRLELEITES
                     VFFDSYEVINERLKRLKENKIGIVLDDFGTGYSSFSRLRELHVDCLKIDKNFVVGISQ
                     KEEDEIITGDIISMAHKLGLLVVAEGVEQEEQKTYLMKHGCDIIQGYYYSKPLSKEEA
                     IKLVFSKN"
     gene            complement(9343..11307)
                     /locus_tag="PLG49_06430"
     CDS             complement(9343..11307)
                     /locus_tag="PLG49_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ16997.1"
                     /translation="MGLFISNNHKKSTSSEGTEDYTYILSGLGINNFIFDMESYEGKH
                     YILMDKGVARLENDSLNYLYKTDMNIEDHSDLAVGEERIIFTKKAEKLIYCLSMNGDL
                     IETISWDLLDQKDMTDIAEIKIKDNYLWILARTDNYYNDILLSLNMENKSVNKVLEDK
                     LFSFDFISDDKFIFSSFIMEEYKTILCEYNLNSKVSKRLMDFSDEIASIWDIKYDAEK
                     NRLVFLSDEGLLEVDLKEKSYKNIFHNRYAEEGRRILSFSLGTPFLAFNGTELFELQK
                     APKKTTTLVILDNKEGVGNILLPNFQAAIREIRKEDDSVECSKMLVENYDETLVKKLL
                     SKHKDFDIFLVRIQDIKNLNPEFLQVIDEIPVVQDTNNILLSGIVKASCVNGRQIGIP
                     ADLWVECWGIDSNLAGELEFDPKESIQWTWDDFLEEAAKHRKDINHDGKADIWLSDWT
                     GPNIVAYIYETSSGTSPLAKEEWRNILIKIKEAQDKGIIIREEEKLAGEEGRFILEYS
                     DLHKYVADHHEADYVFFPNIDPENPVYILDGWIYCINPYSDKIDLAARLLCEAIKPQN
                     YIPTNSSILHNNNAWYNEYNDDEIIWAKQISFDDESFSKYSHVINHAIPKSKSLYETP
                     LYDLVIEYFGNKADVDLVAEELIKSYSFYK"
     gene            11578..12414
                     /locus_tag="PLG49_06435"
     CDS             11578..12414
                     /locus_tag="PLG49_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ16998.1"
                     /translation="MKGKINAITNILFYIGLGLFFLFHIVPVLMNIFFSVIDYGKKIR
                     FLGLQHYILLFKDKLFITAFSNNIIFFLLAIPLMILVAYPLARILSSCGFSFRWIKLP
                     FLIPILVPSAAITHVWSILFGEENFIGKYIFHLSGWKAQWQDILPLLILFIWKNCGYH
                     ILIFMIGFMLIPSEIYEASAIDGASKRQQEFYITLPLMTGPFFISILFTFIQQLKIGR
                     ELLLLYGEVPKESLYLLPYYFREEFFNMNIGRMSSAAVIFSIPILLAFFTLYKFISRK
                     EN"
     gene            12416..13216
                     /locus_tag="PLG49_06440"
     CDS             12416..13216
                     /locus_tag="PLG49_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ16999.1"
                     /translation="MKKKSLLLSLILGSLFLYPIMMTFSNSLMTPEEIALNENQIVFI
                     PQKISIRQYYLLFFERFEYLQMFWKSIFLTVPIVAGNSIYSVFMGSLLAKSKLKYKNA
                     IFLLFVLAMFMPYQITMLPHYMIFKCLSVDGTDWAIILPMIFSPLGVVLITLFIRNIP
                     DELIEAVKIDSSSLWDIFIHGIYPYAQGAIVILIIFTFAEAWNLVEQPLNVYNNLYEQ
                     PLSVLLAYMKYDPEIVFAASVIYIIPIIIAYFYFQESLEEQLQLIKDR"
     gene            13508..13984
                     /locus_tag="PLG49_06445"
     CDS             13508..13984
                     /locus_tag="PLG49_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17000.1"
                     /translation="MEFNPEDCTALEKEILEKIRQSEAKVKSREVKKEQIQNKYDLEL
                     ALLEKEKALLSPVSENHYELQKILHEQEVKKSSYEQAKKQLEAYQSLYESGAISYAEL
                     LSKKEEVKKLEKELEFLSKTYLETLSGLEKTMKKPCSNMKKKSSICKMLKKKNWLL"
     gene            13918..14628
                     /locus_tag="PLG49_06450"
     CDS             13918..14628
                     /locus_tag="PLG49_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17001.1"
                     /translation="MLQYEKEIIHLQNAKEEELAALESEPDPANDYNSLLDLYENIKT
                     LLQTGQVLAPCDGTIKEIQIKEHSYYQGLSELFSIIPVNSSIRYQADISNFDKSYFEN
                     LESAVLLLRNDQIEVEILGITEENGKTYLLFEPSSYLTREQTKEIQSLLITKESSFYD
                     AIIPCRALIGEHVYVLREEDRAFWGKAYYVEKVSVKAGMANMNEVGIEMGLSQGDKVV
                     VSWDRELKDKQRVVLELP"
     gene            14639..15502
                     /locus_tag="PLG49_06455"
     CDS             14639..15502
                     /locus_tag="PLG49_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17002.1"
                     /translation="MKKIKFNPHLMVIALVLLSFCVIQLNPYKNLLQLHQAIDSEQKI
                     INTQNMIAEFENLNEIESVSFYCIQKSRLNNLDIEIVFSDHKRLNFEELNLLKGRFLI
                     SSDINKKNSYAVLDKSLALQLFANLDCIGEEVMINNTNYSIIGIVSYKNKIDHFINNT
                     PKIYLPYPFLSESAEQYIEIQCQNGTVYSLENYICAKYPHLQIMNFQASVNSIFNWIK
                     ILGFAFLLFILWLTIQNLRSDYTLLSDKFQKDLQDYYWSETIKINKKRILKIILMAAA
                     CSIIILLLFQF"
     gene            complement(15774..15986)
                     /locus_tag="PLG49_06460"
     CDS             complement(15774..15986)
                     /locus_tag="PLG49_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17003.1"
                     /translation="MEHENRVDIKNLDKIIFAIMKEYNIDTTKPEGWEKIEDYLDEYS
                     DCLEFANYKMSYEKLCDLFEEAVSTH"
     gene            complement(16148..16510)
                     /locus_tag="PLG49_06465"
     CDS             complement(16148..16510)
                     /locus_tag="PLG49_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17004.1"
                     /translation="MNYKKSIIINDKEKIDYSECKTGNKLIITRDVSHDIIPSSNPIK
                     YEFINEVVRNLITDWDLTLYHWVNDKVGDLEDELESKKDEAVDEDDKLDDEICDLEEW
                     LEEIDGCFLGGKLRMLDE"
     gene            complement(16833..17549)
                     /locus_tag="PLG49_06470"
     CDS             complement(16833..17549)
                     /locus_tag="PLG49_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOQ17005.1"
                     /translation="MLNYQIEWRGNEHTNFSSREGNTPLIIVNHVADASAASCDSWFR
                     SSENKHSSAHFLVTFKGEIRQYVRIEDMAWANGLKLYDIPKAKSSLVRSMKMNPNLYS
                     VSIEHEGTTGRLTEEQFQATLWLHCYIRDYVKNKWGYQIPMDREHILGHFEIDPLKKP
                     DCPGKDFPWERLIKELQKKNTAEQWKYEGIEFLHQYGILSDLEGWKNKINEPMPVWAV
                     CLLIERIIQKYETFKHTQRV"
     gene            complement(18087..18356)
                     /locus_tag="PLG49_06475"
     CDS             complement(18087..18356)
                     /locus_tag="PLG49_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17006.1"
                     /translation="MPKLFKEKIYDNLVQDILVYNVLRLDKNLERFHKSIFSNKPYVD
                     IDVEYCPDCKNAEVLIRHYYKTFLRYELDVLKTEDYKDIDISSAL"
     gene            complement(18340..18981)
                     /locus_tag="PLG49_06480"
     CDS             complement(18340..18981)
                     /locus_tag="PLG49_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17007.1"
                     /translation="MKNTILYGLLGFIISFTICLSIILLMDSIFFSIDILFCIPVGYA
                     FIGYFACKGFNYGIVKDNLPYNPAYRIHLIFMLYVIGYYFLIHYASYFMARIEDGFLV
                     YNLTGDHISNYILEGYGLMNFSKYMRLISEPYSYRFIIFDGPAEIEVSSLLSESQVQE
                     SYNIENIFTLLQMLTGFIGFMFGGVLAAVKPKTMKWCEKCKKYYRKKLLAKAF"
     gene            complement(19276..19348)
                     /locus_tag="PLG49_06485"
     tRNA            complement(19276..19348)
                     /locus_tag="PLG49_06485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19313..19315),aa:Thr,seq:ggt)
BASE COUNT         6384 a         3657 c         2744 g         6688 t
ORIGIN      
        1 aatcgcccct gtaattttac aggcggcgtt ttttatttaa ataataaata ataatatgaa
       61 ggaggaactg aaatgaagaa attaacaagt cttttagcca tcatattatc tttgcttacc
      121 cttgtagtag gttgtggaaa tcaaaatact gactcaaaaa acactgattc tgggaatgta
      181 agtattggca ttatccagta catggatcac gttgctcttg atgctgccag agaaggattt
      241 attgcagccc ttaaagacaa tggttatgtt gaaggagaaa accttagcat tgattatcaa
      301 aatgcccagg gagaccaaag taacctttct acaataagtg atcgttttgt aagcaataag
      361 gtagatttag tccttgccat tgcaactcct gcagcacaat ccattgctgg taaaacaaca
      421 gaaatcccta ttcttgcaac cgctgttacc gattatgaag cagcaaagtt agtagattct
      481 aatgaagcac ctggaggaaa cgttacaggt actaccgata tgaaccctat aaaagaacaa
      541 attgaactcc ttgtaaaatt agtatctgat gttaaaacca ttggtctgct ttatacttcc
      601 agcgaagata actccatcgt gcaagccaac attgcaaaag aagtcattga aagtctcggt
      661 ctttctcacg tagaagtaac tgttaccaat tccaacgatg tacagcaagc aactcaatcc
      721 attgttgagc aatgtgatgc tatctatatc ccaaccgata acacctttgc atcggctatg
      781 cctgtggttc atggcgttac tgctgaatcc aaaacccctg ttatttgcgg ggaatctggc
      841 atggttaaca acggtggcct tgcaacctta ggtattaatt attatgattt aggctatcaa
      901 actggtttaa tggctgttaa agtattaaaa ggcgaagcag aacctgctac aatgcccatt
      961 gaatctgcaa acaaatttga ctttgctatt aatggtacag tagctgaaga aattggaatc
     1021 gaaatacctg atgatctgaa agaatacgtt tttgaagccg aataagaatt ttatctgaca
     1081 gcttaggaaa tttgccaaag aaatggggga atccatatga ctcaaattat tttaggtgct
     1141 gtttccctgg gacttttatg ggcaattatg accataggag tatatattac ctatagaatt
     1201 ttggatattg ccgatttaac cgttgaaggc agcctgcctc ttggtgctgc catagccgct
     1261 tctgctataa gcagtggtgt caatccttac ctcgcaactg gcgtagccat cattggaggc
     1321 ctggctgcag gattaacaac aggaattttg cacaccaaat taaaaattcc tgctctttta
     1381 tcaggaattt taaccatgat tgctttatac tccattaatc taagggtaat gggaaaagct
     1441 aacgtttcac ttttacgaat ggacaccgtt tatacggttt ttcaaaagct tggtttaaat
     1501 gaaaccaatg ctgtaattat tttaggtatc attgttgtat tttcattagt atgtctttta
     1561 tattggtttt ttggaacaga actgggctct gccatccgtg ctacaggaaa caatcccagg
     1621 atggttcgtg cccagggcat caataccaat accatgaaaa ttttaggtct tgttataagc
     1681 aacggtttga tagccttaag cggtgcctta attgctcaaa gccaaagttt tgctgatgtt
     1741 caaatgggta cgggttctat cgtcatcggt ttggcatcgg ttattattgg tgaagttctt
     1801 tttggcaaaa ggagcttctt tagccggtta atttctttag tattaggggc catcacatac
     1861 cgtatcatta ttgcttttgt attaaagatg ggtatgcctg ccaatgattt gaagctcttt
     1921 actgctatta ccgtagctat tgctttggct ctgcccaata ttaaaaaatc actccagcca
     1981 accttttcaa aattcttcag gggaggggaa aacaatgctg tccgttaata atatttacaa
     2041 agtgtttaac ccgggaaccg ttaatgaaaa agttgcttta agaggcataa gtttaaccct
     2101 tgaagaagga gaatttgtta ccatcatcgg tggaaatggg gctggaaaat ctacgctttt
     2161 aaatgctatt gccggcgtat atcccgttga tgaaggttcg attattattg atggcacaga
     2221 tgttacaaaa ttgccggaac acaaacgagc agccttcctt ggccgggtat ttcaggaccc
     2281 catgatgggt accgcttcaa atatgtggat tgaagaaaat ttagccttag cttatcgcag
     2341 aggacaacgc cgtaccttaa gatggggtat taaacaaaaa gaaagagaat attataagga
     2401 attactaaaa actttagaac taggtctgga agacagaatg acaaccaaag tggggcttct
     2461 ttcaggcgga caaaggcaag cattaaccct tttaatggca actttaaaaa aacctaaagt
     2521 tttactattg gatgaacaca ccgctgccct tgacccaaaa actgctcaaa aagtactaga
     2581 gcaaaccgat aaactggttg aagaaaataa cttaacgact ttaatgatta cccataacat
     2641 gaaagatgcc ataagacatg gaaaccgttt aattatgatg catgatggga aaatcatctt
     2701 agatatcaag ggagaagaaa agaaaaacct gaccgttgaa gatttacttg aacgctttgg
     2761 taaagtgagc ggtgaagaat ttgccaatga ccgtgcactt ttgtcttaaa tacaataaat
     2821 caaattttat atgtacccct taagtagata gaaccattaa aaatctactt aagaggtttt
     2881 ttattcttgt cattaatact tcttaatact tctgaatttt atctaaagtt tcacaaatgt
     2941 atccaaataa acttaaaagg aaaccagtga atttatacaa caaagtataa aacagccacg
     3001 taacttcgtg gctgttttta atgctattta tcatattaaa attatcatgg tctaactggt
     3061 agcacatatt tattatatac tctataaagg aatacgaccg cttctgccct gcttgccttc
     3121 ccgtaaggat taatcctgtc atcatatcct acgataagcc cttctcctac cattattgct
     3181 atactattga ttgcatagtc tgaaatctca tttttatcct tatatttatc taatatcttg
     3241 tggtcataaa agtaatcaaa ctctttatat tgccttaacg ctctgtctgt caatgtcatc
     3301 atatcctgcc tcgaaatcgt ttcatcagga taaaatttat tgttttcatc tcccaaagca
     3361 atccctaatt ttcttgctat tcctactgtt tcataataat ccgactctgg taaaacatca
     3421 gcaaaattat tatcaaattc tgctgtcaat cctaaacttc ttattaaata tgttacaaaa
     3481 tctgccctgg taatgctggc atctgctaca aataaattgt ctgtcagccc tgttataaag
     3541 cctttggaag ccataaattc tatagctttc tgagcccatg ctacacgatc aatatcctta
     3601 aaagtcttat gttgccaaac tactgcatat tgtcctgaaa aagtcgttgt aaaatttact
     3661 ttcctttctt gcggatcata ttcggcacta gctactggag tctcatttcc actatgatcc
     3721 attttccata ttgtaatata ttcccaatct aaatttatct ctgctgaagg tttataggga
     3781 atcgatacca tgacaggcct ggtcatattt acttttttgt tacctgtttc caatgtcaat
     3841 tgcacgacag gattgttgcc tgcaagttct tttattttag gcgataaggc tgattcttct
     3901 aaccttgcta tataaatatt tactgacttt tcatcttcta tagtttgtaa aaaaatatta
     3961 taaggaacaa taacctcgcc ttctggtgta gagaaaatca cttttttctc gtcttgccct
     4021 tctttaaata aagtggctgg cagtattaag gtatagctat ttacaccttc aacagcaggc
     4081 actataatca tcaaactttc atcataaccg aaacttttcc ttatgatgtc tatcgtgtcg
     4141 acaactacat tagcagcatt tttatcaatc tccagttctt ttgtaacctt tctttcattt
     4201 tcacctatta tagatacctt gtattttttt agtttttctg taaatacagt aaagtccttt
     4261 tttattgacc tatggtctgt gcctttatct tataggaaac caataagcct ctaatttgac
     4321 atccttattg ataataaaag tatctccggg taaatagaat tgatgatcat aaatccaacc
     4381 tataaataca tatccgcttt tcgtaagttt cccggtatta tcaagaacag tcaccctgtc
     4441 tcctgccata tatagattat aatcttgagg aacagttcct cctgtatgac catttccatc
     4501 ataaataacg gtataccact tttcttttat atcactatca tcaataatat aaatctcctt
     4561 ttccccatac accagggatc catcttgagc agtagctcta acgactacag tacctacaga
     4621 atgagctgtt aacaaaccat tttcatctat gtctgcccca tttcctttga caatactcca
     4681 aaccactgct ttattgccag catcatcagg ctctactatt gccagcatct gtagtttatt
     4741 tccaacgacg actctatcac ttcctcctgc tgctattact gttatagctg acacaggaag
     4801 aaagccgctt tctgcttctt ccactgtcac cagaactttt gcaagtaaag gtattccatc
     4861 tggattgggt aaatcatccg gtaaaataac cacaccttcg acttcaaaag tttgcttttt
     4921 tgtatcatta ggattataag cacattcgtc tactttccat atgatactgg cttgtctttt
     4981 tatccctccg ctaaggatta aggttacagt attaggtaaa cctaaaccct gaaccgtttt
     5041 tacagctccg ttttttatac cagtaacagg ttcaggtgct actatactca taagtttaat
     5101 ctgatgaaca ttgaaattag cccagatacc atcaaaataa ccctgtgctg taggttgaac
     5161 aaaaacgact aaatcagatg cacaattatt aaagatattt tcctcaatag ctggagctat
     5221 taacggttgc attaaaactt ctctaagact agaacaacta tcaaatgccc cactgcctat
     5281 agatgtaacc tgccatggaa tcgttatgga tgtaagattt tcacagttat aaaaggcata
     5341 cgcttctata tccgttacac tctctggaac aattattgtt ctaagtcctt tacagttata
     5401 aaaagcattg gcgcctatga cttttatttt tgactcatct gcaaattcta ctctttcaag
     5461 cgtatcacag tcataaaatg catatcctcc tatgtcaata acattttgag gaatataaat
     5521 tgaactaaga ttattacaag cattaaaagc actctctcct atagtttgaa gactctctgg
     5581 aagaataatt gataccagac ttgtacaacc ataaaatgca aattcatcta tgcttgtaac
     5641 accttctgga ataatgacag attgaagacc tgtacaataa tagaatgtat ttttacctat
     5701 ggcacttata cctgatggtt ccagaaattt aatttctttt aaattatcac aaccataaaa
     5761 tacgtattcg tctaagtcaa taaggctttc aggaaggact attgttctaa gattatcaca
     5821 ttcataaaaa gcatattggc ctattctttt tactccttcc gggatgctta tggacgtaag
     5881 attcatacaa ttataaaatg caaagtcttc tatactggta acgttattcg gtattacaaa
     5941 cgaacgaaga cctgtacaac cataaaatgc tttttgccct atttccctta actgggaatc
     6001 agaagaaaat tgaatgaatt caagattatt gcaatcataa aatacatatt cacctaaact
     6061 tgtaacactg tctggaatga aaattgaata aagacttttg caaccattaa aagcactctc
     6121 ctctatatgc taaacacctt ctgaaataac aacagataca agattatcac ataaataaaa
     6181 tgcatgagaa cgaatcgcaa gaacagttgc cggaatcgtt actgatgtaa ggcttttgca
     6241 atcataaaaa gcatactcac caatacttgt tacagtataa acgattccgt tatattcaat
     6301 agtagctggt atattataaa aactttctgt ataatcatgc cgtataactt caaccgtacc
     6361 agtatcccct tcttccgtaa tcaccctcag tttcaaacca ctttctgaat ccacaaattc
     6421 gctatcaaca gcacccagag gctttaaagg aaataatatc aaaagaaaca ccataataaa
     6481 gctagataat aaactttttg atattatttt tgaattcact ttatcccccc ctaatgaaat
     6541 aataatttat caatgatatt tattattaaa ttttttaggc ctgtatttaa cacctataat
     6601 ctaaaactta ttagttttta gaaaacacca atttgatagc ttcttcttta gaaagaggct
     6661 tactataata ataaccctgt ataatatcac aaccatgttt cataaggtaa gtcttttgtt
     6721 cttcctgctc tactccttca gctacaacca aaagacccag cttatgcgcc atggaaataa
     6781 tgtctcctgt gatgatttcg tcttcttctt tttgagatat gcccaccaca aaattcttat
     6841 caattttcaa acagtccacg tgcaattctc ttaacctgga aaaagaagaa tagcctgtcc
     6901 caaaatcatc taaaacaatt cctattttgt tttctttaag cctctttaat ctctcattaa
     6961 tgacctcata actgtcaaaa aatacagatt ccgttatttc caattccaac cttgaagggt
     7021 caatacctct ttcttttatg atctccataa tattgctgat aaaatcatcc cttaataact
     7081 gtatacccga tatattaaca gccactttaa ccgaatcaat ccccttatcg gtcaggtctt
     7141 ttagaaaatc gcaggctttt ttcaggatca gattgctaag aggaacaatc agttgctttc
     7201 tttcagccag attaataaat tcaagcgaag acacaaaacc aaaagtagga atattcatac
     7261 gtgccaaagc ttcaaaggcg acaattttat tgctccttaa atcaaactgt ggttgaaatt
     7321 caagatacat ttttttgtcg tcttcattaa gaataatgct tctaatttct ttttcgatta
     7381 aatcttccct ttgaagtttc ttttccattg cttcattaaa aaaagcatac tttttagagt
     7441 cattcgattt tatatagctt aatgtaatcg atgcatcttt taaaaccctg tcaatatctt
     7501 tatatctgta cctgtccatt tcaaccatag ccacttgtat atctaaaagc tgttctatat
     7561 cttgtacgac taatgaatta ttaaacaggc ctgttatttc tttcgaaatg gaaatcagtt
     7621 cttcaggttt cttgtaattc tttacaaaca acacaaaacg gtctgcagaa tacctgaaaa
     7681 gaatcctgtt attatcctgt acagttttta acctttcacc caatgctttt aaaatttcat
     7741 ccccatagtc atagccataa actaaattaa ttaatttaaa atgcctgcaa ttaataaata
     7801 aaaaagcttt tttgctgtct tcttttttct ttaattcctc attaattaac tctctaagat
     7861 agtgcttatt cagtaaaccc gtaagagaat catagtaggc taaatggaat aatcttccgc
     7921 tcactttgta aattgaaaac ataataaata ggactaaaca gtaaatcact gttaaaataa
     7981 ccacgcctaa aacagatagt tgttttataa ttgtttctgc attgttcaga gactttacaa
     8041 tagcaatcgt acctattttt tttccttcaa aatataccgg ctgaaaaatc tcatagatca
     8101 tttttccatt ttgttcagtt acaaaactgt acacttcatt atttaaaata gcgggaatca
     8161 ctttttcatt tttaaacttt tctccaatca tatgtgtatc tgaactgcct ataatattaa
     8221 aatcgttgtc cataaaataa attaagctaa tttcttcgcc aactttcatc tcatccagta
     8281 actgctggat attaaacctg tctaaaaagc tgtaaacctt atccgctctt acaccaatct
     8341 ggacaagtcc tccatttgat actttaaaat aaccatattt atagtaatta ccggttatgg
     8401 aatctcgtct tatatgatca attcggctta attcattact tttcataaaa tcataaactg
     8461 ggtgaccttc atatgcctcc cagccaatca attcttccaa attcgaatat attaactttc
     8521 cgcctgcctc ataataatca atttcatcaa cttttaaaat attagccaag cgagcaaata
     8581 gctcattatt atattgtccg tcatataaaa caacggcatc accggcaaca attagccgtt
     8641 cttctaataa ttgattaacc acttccctgg cttctgaagc cttggccagg ctatgggaat
     8701 agccctgggc aagtttgaca gactcctgat ttaaataatg atagtagtgt tccctgacgc
     8761 tatcaaaaac catccgacct gtagcaataa atattattgt aattaaaagt aaggggataa
     8821 taaaaatttt tatgtcaagc tttttcattt tttccattta aaattctctc cttaaggaac
     8881 caataattta taactagcag aagagattct aaaaaaattt atattatcaa aaaataaact
     8941 aactaaagca aataaaagga cattttgccc ttatagccat caaaatgtcc ttaatgctcc
     9001 atggtgctct gtcatatttt agttcgttca ttcgttaaat gattagttga taaaaataat
     9061 tatatcataa tgcaaattga gacacaagaa aacaaaaaaa cgcaaatgaa taaaaaaggg
     9121 acattcatcc aggaatgtcc tgtcaggaaa actaccgcaa tttataatgt tattcttctc
     9181 tatcttcaat tattttaaac attattgctt ctgaagcatt tttaaattta tttgctagtt
     9241 ctgcttgaag tttacctttt tcaactttcc catttacaac tgtttgttta ttggaaaagc
     9301 agttagtggc tgtctgaaca taataaaata taattaacat tatcatttat aaaaagaata
     9361 agactttatc agctcttctg ctactaaatc gacatctgct ttatttccaa aatactctat
     9421 tactaagtca taaagaggtg tttcgtaaag actttttgat ttcggaatcg catgattaat
     9481 tacatgacta tacttgctaa agctttcatc gtcaaaagat atttgtttgg cccaaataat
     9541 ttcatcatca ttatattcat tataccaggc attattatta tgaagaatgc tactatttgt
     9601 gggtatgtaa ttttgtggtt ttattgcttc acataacaat ctggctgcca aatcaatttt
     9661 atctgaataa ggattaatgc aataaatcca tccatctaaa atataaacag gattctctgg
     9721 atctatgtta gggaagaata cataatctgc ctcatgatga tccgcaacat atttatgtaa
     9781 gtctgaatat tctaaaataa accgtccttc ctctcctgcc agtttttctt cttccctaat
     9841 gataatacct ttatcttgag cttctttaat tttaattaat atattcctcc actcctcttt
     9901 cgctaagggg ctcgtaccgg aggatgtttc gtatatataa gctacaatat taggtcctgt
     9961 ccaatcgctt agccatatgt ccgcttttcc atcatgattg atgtctttcc ggtgtttagc
    10021 agcctcttct aaaaaatcat cccaagtcca ttggatactt tccttaggat caaactccag
    10081 ttctccggca aggttagaat cgatgcccca acattcaacc cataaatcag caggtatgcc
    10141 tatttgtctt ccattaacac aagaagcctt tacaattcct gataataata tattatttgt
    10201 atcctgaaca acaggaatct catctatgac ttgcaaaaat tccggattta agttcttgat
    10261 atcctggata cgtaccagaa agatatcaaa gtctttatgt ttagagagca actttttaac
    10321 aagcgtctca tcatagtttt caaccaacat tttgctgcat tctacactat cgtcttcttt
    10381 tctgatttct cttatagccg cttgaaagtt tggtaataat atgtttccaa ctccctcttt
    10441 attatccagt ataaccagtg ttgtagtctt tttgggagct ttttgaagct caaaaagttc
    10501 tgttccgtta aaagctaaaa aaggcgttcc taaggaaaaa gacaaaattc ttcttccctc
    10561 ttcagcatat ctgttatgaa aaatattttt ataacttttt tcttttaaat ctacttccag
    10621 caatccttca tcactcaaga aaacaagcct gtttttttct gcatcatatt ttatatccca
    10681 tatacttgct atttcatcac tgaaatccat taatctttta cttacttttg aatttagatt
    10741 atattcacaa agaatcgttt tatattcttc cattataaaa gaagaaaaga taaatttatc
    10801 atctgaaata aagtcaaaac taaataattt gtcttctaag actttgttta cacttttatt
    10861 ttccatattc agactcaata gaatatcgtt ataataatta tcggttcttg ctaaaatcca
    10921 taaataatta tctttaatct taatttcagc aatatcagtc atatcctttt gatctaatag
    10981 atcccatgaa atggtctcta tcagatctcc attcatgctt aaacagtaaa taagcttttc
    11041 agcttttttt gtaaatatta tccgctcttc tccaacggca agatcgctgt gatcttctat
    11101 attcatatcc gttttataaa gataatttaa gctgtcattc tccaatctgg ctacaccttt
    11161 atccattaag atataatgct tcccttcata agattccata tcgaaaatga aattatttat
    11221 ccctaaacca ctcagtatat aggtgtaatc ttctgtccct tcactgcttg tacttttttt
    11281 atggttatta ctaataaata aacccactat cattaatcct ataattccta ttaccccaaa
    11341 aaaatacaaa attttttcga ctgcatttgt gacctccaaa tcattaaaca cttaaaaaat
    11401 aaaaatattc tagtctctta ctcttttata agttgcatta ataaataatt acaaatatta
    11461 acattaacag gataatacac aatagtaata ttgtcaatat aattgttaaa tatttgactt
    11521 tttggctatt ttatgttata ttgctttcat aattttacaa tatctgaggg atatgatatg
    11581 aaaggcaaaa taaacgctat tacaaatata ttgttttaca tcggactggg tttatttttt
    11641 cttttccata ttgttcctgt attaatgaat atattttttt ccgtaattga ttatggtaaa
    11701 aaaattcgtt ttctcggact acaacattat atccttcttt ttaaagataa actttttata
    11761 actgcctttt ctaacaatat tatttttttc ttattagcta tcccattaat gattcttgta
    11821 gcttatcctt tagccaggat actttcttcc tgtggctttt catttagatg gataaagctt
    11881 ccttttttaa ttccaatact ggttccttct gcagcgatta ctcatgtctg gtcaattcta
    11941 tttggggaag aaaattttat aggcaaatac atctttcatt tatccggctg gaaagctcaa
    12001 tggcaagata tactgcctct cttaatactt tttatatgga aaaattgcgg ctatcatatt
    12061 ttgattttta tgattggctt tatgctcatt ccatcagaaa tctatgaagc cagtgctatc
    12121 gatggcgcca gcaaaagaca acaggaattt tatattaccc ttcctcttat gactggaccc
    12181 ttctttatta gtatcttatt tacatttata cagcaattaa aaattggcag ggaacttctg
    12241 cttctctatg gagaagttcc aaaagaatcg ttatacctgt tgccttatta cttcagagag
    12301 gaatttttta atatgaatat cggcaggatg agcagcgccg ctgtaatctt ttcaatcccc
    12361 attttgcttg catttttcac tttatataaa tttattagcc gcaaggagaa ttaggatgaa
    12421 aaagaaaagc ttacttttat ctttaatctt aggctcttta tttttatatc ccataatgat
    12481 gacctttagc aattcgttaa tgactccgga agaaattgca ttaaatgaaa accagattgt
    12541 ttttatacca caaaaaatca gcataaggca atattacctt cttttctttg agcggtttga
    12601 gtaccttcag atgttctgga aatctatttt tttaacggta ccaattgtag ctggaaacag
    12661 tatttattct gtttttatgg gcagcctctt agcaaaaagc aaattaaaat ataaaaatgc
    12721 tatctttctt ctttttgtat tagcaatgtt tatgccctac caaatcacta tgctgccaca
    12781 ttatatgatt tttaagtgtt tgtctgtcga cggaacagac tgggcaatta ttttaccaat
    12841 gattttttca cctttgggtg tggttttaat taccttattt atacggaata tccctgatga
    12901 actaatagaa gccgtgaaaa tcgattcttc atccttgtgg gatatattta tccatggtat
    12961 ttatccttat gcccaaggcg ccattgttat cctgattatt tttacttttg cagaagcctg
    13021 gaatctggtt gagcaaccct taaatgtcta taataatctc tatgaacaac ccttatcggt
    13081 tcttttagct tatatgaaat atgacccgga aattgtcttt gcagcttccg ttatatatat
    13141 cattccaatt attattgctt acttttattt tcaagagtcc ttagaagaac aattgcaatt
    13201 gataaaggat aggtaggtgc catatgaaca tcaagctaaa atggacaaaa aaattaagca
    13261 ttagcttttt actttttcta ttctttttta ctttttgctc ccgcagtatt ttttatatac
    13321 tgaccccaaa agtccagata gaaagtacaa aaagcggaaa aatcattaat catcaatcct
    13381 ttaacacaat tgaaacttat tttactgatg tagacagcat acggttgcct tataactttg
    13441 aaaaaggctt gtatgtaaaa aaatccttgc gtaccagggc aaaaatgtaa aaaaaggcga
    13501 agttattatg gaatttaatc ctgaagattg tactgcctta gaaaaagaaa tcctggaaaa
    13561 gatccgtcaa tcagaggcta aagtcaaaag ccgggaagta aaaaaagagc aaattcaaaa
    13621 taaatatgac ttagaacttg ccttattaga aaaagaaaaa gcccttttgt caccagtttc
    13681 agaaaatcat tatgagcttc aaaaaatttt acatgaacaa gaagtcaaaa aaagcagtta
    13741 tgagcaagca aaaaaacagc tggaagcata tcaaagttta tatgaatcag gggcaatctc
    13801 ctatgctgaa cttcttagca aaaaagaaga agtaaaaaag ttagaaaaag aattggaatt
    13861 tttatcaaaa acttacctgg aaaccttatc cggacttgaa aaaactatga aaaagccatg
    13921 ctccaatatg aaaaagaaat catccatttg caaaatgcta aagaagaaga actggctgct
    13981 ttagaaagcg agccggaccc tgctaatgac tataatagtt tactggatct ttacgaaaac
    14041 ataaaaaccc tattacaaac cggacaagtg cttgcccctt gtgatggcac aataaaagaa
    14101 attcaaatta aagaacatag ttactaccag ggattaagtg aattattttc catcattcca
    14161 gttaatagtt ctatccgtta ccaggcagac atcagcaatt ttgataaaag ctattttgaa
    14221 aatctcgaat ctgctgttct tcttttaaga aatgaccaga tagaagttga aatattaggt
    14281 attacagaag aaaatggaaa gacttatctt ttgtttgaac caagcagcta tttaaccagg
    14341 gaacagacaa aagaaataca gtccctgtta ataacaaaag aatcttcttt ttatgatgcc
    14401 attattccct gccgtgcatt gattggggaa cacgtttatg tcctgcgtga agaagacaga
    14461 gctttttggg gaaaagctta ctacgttgaa aaagtttccg taaaagcagg aatggcaaac
    14521 atgaatgagg taggtattga aatgggcttg tcacaagggg acaaagttgt tgtctcatgg
    14581 gacagagaat taaaggataa acaacgggtt gttttagaat taccctagag ggaatctgat
    14641 gaaaaaaata aaattcaatc cacatttaat ggtcattgct ttagttcttt tatctttttg
    14701 cgttattcaa ctaaatccat ataaaaatct tttgcaactt catcaggcaa tcgattctga
    14761 acaaaaaata attaacacac aaaatatgat agccgaattt gaaaacttaa atgaaatcga
    14821 atcagtctcc ttttattgca ttcaaaaaag ccgtttaaat aatctggata ttgaaatcgt
    14881 cttttcagac cataaacgat taaactttga agaattaaat cttttaaagg gcagatttct
    14941 tatctcttca gatatcaaca aaaaaaattc ttatgctgtt ttagataaat ctttagcatt
    15001 gcaacttttt gccaatttag attgcatcgg agaagaagtc atgataaata atacaaacta
    15061 cagcatcatc ggtatcgtca gctataaaaa caaaatagat cattttatca ataacacacc
    15121 taaaatatat ctgccctatc cttttctttc agaaagtgct gaacaataca ttgaaattca
    15181 atgccaaaat ggaacagtat attctttaga aaattatatt tgtgcgaaat atccacactt
    15241 acaaataatg aatttccagg caagtgtaaa cagcatcttt aactggataa aaatacttgg
    15301 atttgccttt ttattgttta tcttgtggct tactattcaa aatctgcgtt ctgattatac
    15361 tttactttct gataaattcc aaaaagacct gcaggattat tattggtcag aaacaattaa
    15421 aataaataaa aaacgaatac taaaaattat tctaatggct gctgcttgtt ctattattat
    15481 tcttctttta tttcaatttt gatttgccat caatccaaat aaaattcctc ctacttttag
    15541 cagctattca ataataaaag aaaagagctt tgaaaatatt aatgaaatca aatacagcac
    15601 tcaaaaactc acgccaaaaa aaataaagtt agaacttatt tgccttctgt caaatctatc
    15661 tttttttctg attatatttg cttttgtccg ctacataagt caatcaaaag aacaaagagc
    15721 tgcataaaaa gaatccctgc ttttatgcag ctcttttcta ctttattaag cctctaatga
    15781 gtgcttactg cctcttcaaa taaatcgcaa agtttttcat aagacatttt ataatttgca
    15841 aattcaagac aatcactata ctcatcaaga tagtcttcga ttttttccca cccttctggc
    15901 tttgtagtgt ctatattgta ttccttcata attgcgaata taattttatc aagatttttg
    15961 atgtcgactc tgttttcatg ttccatcgat tcaacatata aatctgtaaa ttttttgcaa
    16021 tatcaagatt tctgtctaat atttcgtgat ttttgattaa aacatatttt tgtattgcct
    16081 tcataaccaa aacagaaagc tcttctttta attgatctat ctcatctata tcactacaaa
    16141 cgtcaaacta ttcatctagc attcttaatt ttcctcctaa gaaacatccg tcaatttcct
    16201 caagccattc ttcaaggtca caaatttcat catcgagttt atcatcctcg tcaactgctt
    16261 cgtccttctt agattccaat tcatcttcta aatctcctac tttatcatta acccaatgat
    16321 ataaagttaa atcccagtcc gttataaggt tccttacaac ctcattaata aattcatatt
    16381 ttattggatt agaagaaggt atgatatcat gactgacatc tcttgtaata atcagtttat
    16441 tccctgtctt acattctgag tagtcaattt tttccttatc atttattata attgattttt
    16501 tataattcat tttacttctt ccctatttta ttttaggagt gaactcttta tgaaacgcgt
    16561 ttcttgctct aacaataatc caggatctct tcatggccaa tatgtcaggc ataaattcag
    16621 aaatagtctc aaaacggaat gttttttact atttttaaat gagtattttt taataatatt
    16681 tttataaaaa attaaagtaa aaattaatta aaaatgtttt ttccaaaaaa tcgatttttt
    16741 aacacacata gaatttctgg cccttttgta aaattgcaca aggatttttt gaattttatt
    16801 ccatatactt cttcccaaat gacaaacatg ttttagactc tctgtgtatg tttaaatgtt
    16861 tcatactttt gtattatcct ctctattaat aaacacacag cccaaacagg catgggttcg
    16921 ttaattttat ttttccatcc ctctaaatcg ctcagtattc cgtattgatg taaaaattct
    16981 atgccttcgt atttccattg ttctgcagta ttcttttttt gtaattcttt aatcagacgc
    17041 tcccagggaa aatctttacc aggacaatca ggtttcttaa gaggatcaat ttcaaagtgt
    17101 cccagaatat gctccctgtc cataggtatt tgataacccc acttattttt tacataatcc
    17161 cggatatagc agtgcagcca caaagttgcc tgaaattgct cttctgttaa tcggcctgtt
    17221 gtaccttcat gttctattga tacggagtat aaattgggat tcattttcat gcttcttaca
    17281 agtgaactct tcgctttagg aatatcatag agctttaaac cattagccca ggccatgtct
    17341 tcaatacgga catactgtct gatttctccc ttaaaggtta ctaaaaaatg tgctgatgaa
    17401 tgcttgttct cactcgatct gaaccaactg tcacaacttg ctgcagaagc atctgcaaca
    17461 tgattaacaa tgattaaagg agtatttcct tctctggatg aaaaatttgt atgttcattg
    17521 cctctccatt caatttgata attaagcatc ttccatcccc tttctgattc attctttttt
    17581 cttattataa tttttctttc tgtgaaaact caaaataaat ctttctgatt ctcttcccgt
    17641 aaaaaaataa ctgtccactg gacagttatc tttctcccgt attatttttg tttgattata
    17701 tttttattct tctaataata agtgaaatga tattccatac aataaaaagt acaccaacta
    17761 ttattaagac tatactgcta tagtttccta ctgaagcttc tcccgaatta aaaatattat
    17821 cgaatgctgg agttaataaa tacttatggg ctattcctaa aataatcaaa cagatgccta
    17881 aataaagcat ataaattttt ttaaaacccc tgaatttaat aaacaaattt tgtcctcttg
    17941 attcattttt ctttctatga taataatcaa attatccttt actatcaatg ctattgggat
    18001 aaattaggca ataatatcaa atgaaagggg cctggaaata tttaaacgga cttttattcc
    18061 tatgtaaatg attaagaaag atgatcttat aaagctgatg aaatatcaat atctttataa
    18121 tcttctgttt tcaaaacatc taattcatat cttaaaaaag tcttatagta atgcctgatg
    18181 aggacttcag catttttaca atcagggcag tattctacat caatatcaac ataaggctta
    18241 ttcgaaaaaa tacttttatg aaatctctcc aagtttttgt ctaatcttaa tacattataa
    18301 accaatatat cctggactaa attatcataa attttttcct taaaaagctt tggcaagcaa
    18361 tttcttcctg taatattttt tacatttctc acaccacttc attgttttag gcttaacggc
    18421 agccagaaca ccaccaaaca taaagccaat gaagcctgtt aacatttgca gcaaagtaaa
    18481 tatattctct atattatagc tttcctgaac ctggctttca gataaaagac tggatacctc
    18541 aatctcagca ggaccatcaa atataataaa tctataagaa tacggttctg aaatcaatct
    18601 catatactta gaaaaattca ttaatccata gccctctaaa atataattgc taatatgatc
    18661 tccagttaaa ttgtatacta aaaaaccatc ctcaatcctt gccataaaat aagatgcata
    18721 atggattaaa aaataatatc caataacata caacataaaa ataagatgaa tcctataggc
    18781 aggattatat ggtaaattgt ctttgacgat tccataatta aatcctttgc aggcaaaata
    18841 cccaataaaa gcatacccca caggtatgca aaataaaatg tctattgaaa agaaaatact
    18901 atccattaaa agaatgatcg ataagcaaat agtaaaactt ataataaaac ctaacaatcc
    18961 atataaaatc gtattcttca ttgcaatcct tccttttacc atacttattc atagattgaa
    19021 aattcataaa cgcaaataat tatagcatat tttaataatt aatcaataat aactcaaatt
    19081 acacttaaat tctcagtctt ttacaagagt ttctaagtat aattcacaat gacgatttta
    19141 ataatttagt gttgtcatct tttttaaata atcttctaaa taaaacactt aatctcattt
    19201 tatttctgac attcaggcaa aaaggggtta aaagcaaaaa aattccagtt aaatactgga
    19261 atcttgattt tgtgaagctg ctaacgggat tcgaacccgt gacctccgcc ttaccaaggc
    19321 gacgctctac ctcctgagcc atagcagcat atttattaaa ttcttgccta acgcttagat
    19381 atattatcat gtttctcgct ttaagtcaag cattttatag gctttttctt aagtttaatg
    19441 ctggaaaata tgataaaagt tagatgctgt tta
//
LOCUS       DAOSUD010000040        19343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1817_length_19343_cov_23.626141, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000040 DAOSUD010000000
VERSION     DAOSUD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_19343_cov_23.626141"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..965
                     /locus_tag="PLG49_06490"
     CDS             195..965
                     /locus_tag="PLG49_06490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ17008.1"
                     /translation="MLQNNDYYKLIHGDYIKIMDSFPDKCFNMIFADPPYFLSNGGIT
                     CKNGKMACVDKGEWDKSKGLTNDFDFTHTWLKNCDRVLKDDGTVWISGTYHNIHMVGY
                     ILNLMGYYIINEISWIKPNAPPNMGCRCFTASHETLLWAKKDKRQKHIFNYKFMKELN
                     EGKQMRSFWIIPTTPKREKIFGKHPTQKPEKLLYRCIMACTNEGDLILDPFCGSGTTG
                     VVAIKNKRRFIGIDINQEYLEISRKRIEDCITEETYEL"
     gene            955..1860
                     /locus_tag="PLG49_06495"
     CDS             955..1860
                     /locus_tag="PLG49_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOQ17009.1"
                     /translation="MSYNVKPILKWAGGKTQLLPEIIANLPDDYKLLRRYVEPFVGAG
                     SVFFYFINNNCFEEYIINDINEKLMNLYKVIRDDCEGLISELYRLQEEYIMLEDLVKK
                     EKFYYECREQFNLNQVSDTKMSALLIFLNKTCFNGLYRENSQGLFNVPFGKRNSPQIF
                     EEKNIKEISRLLNIKNKEGKYRVHILNKSFEDLDDYIDNNTFVYFDPPYRPVTFGGFN
                     SYNKSSFNDESQIKLRDFFVTQDKKGARLMLSNSDPKVLDKNDNFFDDLYKDFTIKRV
                     TANRMINSKGSGRGPINELLIMNYD"
     gene            1965..2762
                     /locus_tag="PLG49_06500"
                     /pseudo
     CDS             1965..2762
                     /locus_tag="PLG49_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016443.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
     gene            2840..3613
                     /locus_tag="PLG49_06505"
     CDS             2840..3613
                     /locus_tag="PLG49_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOQ17010.1"
                     /translation="MLETYRPREDILQSTTLWSFPDRGDWATHNNKYRGNWSPYIPRN
                     IIKRYSKVGDMVLDPFVGGGTTLIEAKLLNRNAIGIDINPEAIKITKQNLNFKYKTSS
                     RIYLLCRNANNLDFIKNNSIDLICTHPPYANMIKYSKNIEGDISLCSPDDFLMQLKIV
                     AHELYRVLKANKYCAFLMGDIRKNKNIIPLGFMSMKVFQETGFLLKEIVIKEQHNCKS
                     TPKWISKSSQYNFLLIAHEYLFIFQKPDPSICLDKSVLK"
     gene            3683..4390
                     /locus_tag="PLG49_06510"
     CDS             3683..4390
                     /locus_tag="PLG49_06510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOQ17011.1"
                     /translation="MTKVIIIDDIRIEVVKKKIKNIYLYVHPPNGKVKISAPLKMKDE
                     AIRNFVISKLAWIKKQQAKFQAQEGQAETEYVSGERHYYQGIPYILNVMHTNKKQRVE
                     ISKKQINLFIRPDSTKEQRERILLEWYRSELKKQIPGLISKWENIIGVKVNSWGVKLM
                     KTRWGTCNPKAKRIWINLELAKKSPRCLEYIIVHEMVHLLEKHHNKRFYAFMKQFLPN
                     WREIKAELNGLKLESPK"
     gene            4518..4781
                     /locus_tag="PLG49_06515"
     CDS             4518..4781
                     /locus_tag="PLG49_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17012.1"
                     /translation="MKICSNEDISKEKVMELFKLHWGSPMMVISTGVYNCSELDAFAA
                     VNEEGETIGLITYLIKDNECEIISLEGGDGIPIRDEIELIKWI"
     gene            4875..5288
                     /locus_tag="PLG49_06520"
     CDS             4875..5288
                     /locus_tag="PLG49_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ17013.1"
                     /translation="MWVLFAILSAVFAALTSILAKIGIEGVNSNLATAVRTVVVVIMA
                     WLMVFITDAQGGLSAISKKSWIFLILSGLATGASWMCYYKALQTGEASKVVPIDKLSV
                     VITVVLAFIFLHEKVDAKTIIGCIMITAGTLVMVL"
     gene            complement(5413..5925)
                     /locus_tag="PLG49_06525"
     CDS             complement(5413..5925)
                     /locus_tag="PLG49_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ17014.1"
                     /translation="MSFLELAKKRYSVRSYLDKPVEKEKLLQVLEAARIAPSAVNKQP
                     WHFIVVTDKIIKNKIAETYPRDWFKTVPAVIVVCGDHSKSWKRNDGKDHCDIDVAIAI
                     DHMTLAATDLGLGTCWVCAFDAKQCHKALELPENLEVVALLPIGYPADTAPESKKRME
                     LSEIVSWEKY"
     gene            6298..7209
                     /locus_tag="PLG49_06530"
     CDS             6298..7209
                     /locus_tag="PLG49_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ17015.1"
                     /translation="MDIMLEILKFIQSFSNPFFDFLFELVTMLGEDYFFILVTGLFFW
                     CFDKYFGYKLAYIYLTGGTVNTMIKEIVKFPRPIGYEGIRSLRVGTAGGYSFPSGHTQ
                     QATSLFTTLMINFKKKWLYLIGTGAMLLVGFSRMYLGVHWPTDVAGGLLIGLVWTYLA
                     IKVFDWSKDKENPVLLGILVIPMFICLLFFKTATYYKAIGTLTALWIGYIIEDKYIHY
                     ETKAVCWKQVIKGIIGFLGLIVIKVFVKKLLPENIYSDFVRYAFMGFWMTIISPLLFR
                     ISNLEERGALGKPKSYTEVTFTKELNS"
     gene            complement(7263..8663)
                     /locus_tag="PLG49_06535"
     CDS             complement(7263..8663)
                     /locus_tag="PLG49_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOQ17016.1"
                     /translation="MKNKFTRKILSFLLFFSIISQFWLPLYANDQLTQKAITYHSWAA
                     PTLFEGERLELIPIEWYDNMLAPVDQAALNTLLNNVDNKLSKLGFPASGSFNNYEADN
                     NITRETVLEGLYHQVLKYELAETVPETASHRKAAIDFMLQKSVLRGDGNNYNLDKVCT
                     LEEAALFATRLVETLCKEANVGSKGFFWKVSDDDTTVYLLGSVHVAKHDIYPMSTKIK
                     EAFKNSDALAVEANIISDQEGILYLQEKMQCPDGTTIYDYISEETAAKLDNVMANIGM
                     KKEQYMYLKPWALSQQLSQLLVTKEGGVGSSPYSGTLGIDIYFIINALYSNKPILELE
                     SIKYQADMLDSFSPEYQEKQLAETLKGFEDPALKNESAEILDTWLNQWIEGDTEAFCK
                     SYFNSIEGDEETKEEFTEKTFTLRNNNMTEAIKQYLDSGNDKSYFVVVGSGHMIGDTG
                     IVAQLEALGYEVEAIK"
     gene            complement(8718..9314)
                     /locus_tag="PLG49_06540"
     CDS             complement(8718..9314)
                     /locus_tag="PLG49_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOQ17017.1"
                     /translation="MQVLMKRILLLPRKQYCSWIALNIYLLLLQWSDITNLLGFAFLI
                     NFVINVTIREYDHKNSSAKLINSLPVTRKEIVRSTFLLTLLTLPVCYAFNYLLYLIFS
                     SAGIRFNIGNLRQNMVIAFIGLMIHSLWILLSFMIEKSLPLYFLIGFFVPLFMFNAEW
                     GIIKDFLSHQLIPLGIVFILWVSFSYFLSLRIYLKKEL"
     gene            complement(9329..10012)
                     /locus_tag="PLG49_06545"
     CDS             complement(9329..10012)
                     /locus_tag="PLG49_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOQ17018.1"
                     /translation="MNKTTWKIILKDFYITRKIWCLLIIPYLIHFILLEALITSSTGK
                     SIFYPYLCSGLYILILIACAQYLDAKHDFLIHSLSIKRTEQIRAKYAFVIIMGLISFL
                     LTGITGAILKKTGLSIMNFASLTDLMLIIAMLTATTALTIPISFLIKALWGLVANISI
                     LLTSIFVILVLPIDITVDSINPWLLLILQIKKLCNNPHNIYYFFIISFALFLISYLLT
                     NKLYTNKDL"
     gene            complement(10005..10880)
                     /locus_tag="PLG49_06550"
     CDS             complement(10005..10880)
                     /locus_tag="PLG49_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17019.1"
                     /translation="MNNILEVQNINKSYKDFSLKNVSFELKEGYIMGFIGPNGAGKTT
                     VIKLIMNLIKKDSGSIKVFGKDHIKNEKEIKDRIGFVYDDNCYYEEFKLYEIKRIIAP
                     FYSKWNDDLFNKYIRQFELPLDKKLKKLSKGMKMKFSIAMALSHDPDLIIMDEPTAGL
                     DPVFRAELLELLLELMQNENKSIFFSSHITSDLDKIADYITFIYDGEILFSRTKDEIS
                     ESYGIVKGANELLNEEIKKSFIHCTQSPYGFEALTMDIPKIRQEFGDSVIIERPSLQD
                     IMVHMHMARRQAGNE"
     gene            complement(10873..11256)
                     /locus_tag="PLG49_06555"
     CDS             complement(10873..11256)
                     /locus_tag="PLG49_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ17020.1"
                     /translation="MKIILSNSSDKPIYEQIVEQIRSQILYGELSEGTQLPSIRKLAQ
                     ELRISVITTKRAYEELEREKLIETVAGKGSFVASQNKELMREKKLAEIENKLSEAIKD
                     SKLLNMTLDDLIEILRFLYEEDKHE"
     gene            11299..11640
                     /locus_tag="PLG49_06560"
     CDS             11299..11640
                     /locus_tag="PLG49_06560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOQ17021.1"
                     /translation="MNSIYVIHAMSSSNKNFLNFCVGNVGQSVEAQVHGALEDMENRL
                     KEIGLTLESVVKVDVLLRDPWDIPVMEKVFRERFAGKYPARKTIQTEFAHVGGPDGLH
                     VQIDTIAYKKK"
     gene            11925..12737
                     /locus_tag="PLG49_06565"
     CDS             11925..12737
                     /locus_tag="PLG49_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOQ17022.1"
                     /translation="MIGAIIGDIIGSRFEFNNKKTKDFELFAKECRPTDDSLMTLAIA
                     KAIMEAEKIIRDSRDEDYYTHIEKMTVLCMQELGRKYPDAGFGGMFFKWLFSDNPMPY
                     NSFGNGAAMRISPVGFAAKTEEEACKLSKIVTGVTHNHKEGLKGAEAVAVAIYMARHG
                     AGKDEIKKKIEENYYDLDFRLDDIRPSYQFDVTCQGSVPQAIKAFLESISFEDAIRNA
                     VSLGGDSDTIGAITGGIAEAYYGVPGEFKEKALSYLTEDLRLIVKEWYDFIA"
     gene            12755..13153
                     /locus_tag="PLG49_06570"
     CDS             12755..13153
                     /locus_tag="PLG49_06570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOQ17023.1"
                     /translation="MVKIIKVTAGVIVDKGKVLITRRAPEDCLAGGWEFPGGKIEANE
                     TPEECLVRELKEELNINVSVDQFCTEVSHSDGDRQINLMAFYCTILDGEIQISVHDKF
                     KWIPVNDLLKYDLLPADVLIAKKVIEEFNA"
     gene            13345..13926
                     /locus_tag="PLG49_06575"
     CDS             13345..13926
                     /locus_tag="PLG49_06575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ17024.1"
                     /translation="MLHMIKENFIKSIGRLFRLLLLVLFFIFVLAALLYFVTGKIYFN
                     FVLLFSVLFAMAISISFLEHKDFFRVGLAFRARDIVCGMIAFAGTAVFYAIFIVMESF
                     INGKNLFPQYLDRITGHFSRSFLSVLLIPLTEEMLHRGYILGNCFQSLKCWQRSLLSA
                     FIFSLTHWVYMPGTDMYYFLLVNVVSTFVLGFF"
     gene            14294..15085
                     /locus_tag="PLG49_06580"
     CDS             14294..15085
                     /locus_tag="PLG49_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ17025.1"
                     /translation="MNKLNIKKIMALFVSLTLIFALAACGLKGKGDLSYVKNKGTLII
                     GYTNYAPMNYYDEDGNFTGFDTELAILVCEKLGVQPEFVEINWDTKEVELNAKSIDCI
                     WNGLTIDDERKATMEITKPYVKNAQVVLMKEGASYTGTESLIGKTVVAEQGSAGEKTI
                     LEDANLSQANYVPKILQTDCLMELKAGTVDAAVLDLTLAKTMTGEGTSYQDIVIVDEL
                     AEEYYGVAFRKGSDICEEVNKIFDEFLADGIMEALAEKYGLELAE"
     gene            15173..15835
                     /locus_tag="PLG49_06585"
     CDS             15173..15835
                     /locus_tag="PLG49_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOQ17026.1"
                     /translation="MNEISVIISRLSESFLLNCKLFALTLIFSLPLGLLIAFGSMSRF
                     RPLRWLVRTFVWIIRGTPLMLQLIIMFFGPGLLGLSFSWPGGASGRFIAATVAFIINY
                     ACYFSEIYRGGIESIPKGQYEAGQVLGMTKPQIFFHVTLLQVIKRIIPPVGNEVMTLV
                     KDTSLARIIANKEIIMMAGEYTNKGLIWPLFATGIYFLVFIGVLTILFNWLEKKLSYF
                     RT"
     gene            15865..16650
                     /locus_tag="PLG49_06590"
     CDS             15865..16650
                     /locus_tag="PLG49_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ17027.1"
                     /translation="MAILEVKGIEKSFEDTHVLKNINFSLEKGEVMAIIGSSGSGKTT
                     LLRCLNFLERPDKGSIIVNNEVIFDATNPSTQKESELRKKRLHFGLVFQNFNLFPQYT
                     VLKNVTLAAELLAKERPDYKQNKKAITEEIQERAKMLLAQVGLSDKLDFYPHMISGGQ
                     QQRVAIARALALEPDILCFDEPTSALDPELTGEVLKVIKNLAERDTTMIIVTHEMDFA
                     RDVADRVIFMDDGVIVEQGSPQEVFGNPKEERTKQFLARYNKT"
     gene            16961..17500
                     /locus_tag="PLG49_06595"
     CDS             16961..17500
                     /locus_tag="PLG49_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17028.1"
                     /translation="MGVKILISLIAMILIYMIDYIKRKEMKEVVIRYSKFGRLQFKKN
                     LFSDNENDLISYNQKIRFGKSNPTIQLKDYQKEKLYVYLHALNYIHEIQNEIVENMCK
                     EALDYCQRWFKLDEDGNPVRYEYIRKSFDVDTIILKIENSVVMAIIRGFCDGLIDDQD
                     IVVYINCTDKEIVKYSIEE"
     gene            17695..18036
                     /locus_tag="PLG49_06600"
     CDS             17695..18036
                     /locus_tag="PLG49_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17029.1"
                     /translation="MFDLYQKTLSCLFELQNEITENLYQETIKYCDEEGIRNSSDNPI
                     IYVTDHFIIDEIMINTIDGQIMLTVWGRAYNEYGTIYSNPMKIYISMHMYIIQRNSSC
                     MTDHIMTGKKS"
     gene            18310..18816
                     /locus_tag="PLG49_06605"
     CDS             18310..18816
                     /locus_tag="PLG49_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17030.1"
                     /translation="MYRDIEILETTAKVIISRFLGIPVGRKFEAYVRFRMKDGKKYNI
                     NMNAMATFGISFGRNPRQYEELYPKVFEAVYAIVAKAMAQPYINKIRSGATVEVAGLM
                     INATAAKPVKSRKDIVINSSNYREAVISQGYGVTVYNKQGDVLWDSPYFNYKNILLIP
                     HILNEIFA"
     gene            18832..19269
                     /locus_tag="PLG49_06610"
     CDS             18832..19269
                     /locus_tag="PLG49_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17031.1"
                     /translation="MAKVKCHICKKSNTDSVCSRCGAHIGDTASERLVLATSGAILYN
                     DDAGKYVDKFSYCKVAMTDRRLIIYNIKPDSDNPAWWFFRSIKNLFEKFPCISINLND
                     IEQVRRYREWFAVKSKTGTYSICVSKQKELDQLFTPYKDPEEQ"
BASE COUNT         6543 a         2686 c         3760 g         6354 t
ORIGIN      
        1 attgggtcaa tttttaaccg gcgattcggt caaaaatagc ccggcgttga caataaaaat
       61 atttttctca aaatattgga catgttatag taaaacaaac atataatatc ttatttttat
      121 ttaacatatc cttttaattt aataattatc gttgtatgat aagattagaa tgtatattgg
      181 aaaggatatc attcatgtta caaaataatg attattataa gctaattcat ggagattata
      241 ttaaaatcat ggatagtttt cctgataaat gttttaatat gatttttgct gatccacctt
      301 attttctcag taatggtgga attacttgta aaaatggaaa aatggcatgc gtggataaag
      361 gtgaatggga taaatctaaa ggcttaacta atgattttga tttcacacat acttggttaa
      421 aaaattgcga tagagtttta aaagatgatg gaactgtttg gatttctgga acataccata
      481 atattcatat ggttggttat atattaaact tgatgggtta ttatattatt aatgaaatat
      541 catggataaa gcccaatgca ccgcctaata tgggctgccg ttgttttact gcaagtcatg
      601 aaaccctttt gtgggctaag aaagataaaa gacaaaagca tatatttaat tataaattta
      661 tgaaagaatt aaatgaagga aaacaaatga gatcattttg gatcatacca acgactccta
      721 aaagagaaaa aatttttggg aaacatccaa cacagaagcc agaaaaactg ctttatcgtt
      781 gtattatggc ttgtacaaat gaaggagatt tgattcttga ccctttttgt ggttccggta
      841 caactggagt agtagctatt aaaaataaga ggagatttat aggaattgac attaatcaag
      901 aatacttgga aataagtaga aaaagaatag aggactgcat tacggaggag acatatgagt
      961 tataatgtta aaccaatttt aaaatgggca ggcggaaaaa cacaattatt acctgaaatc
     1021 attgcaaatt tacctgacga ttataaattg cttagaaggt acgttgaacc atttgtggga
     1081 gctggttcgg tattttttta ttttattaat aataattgtt ttgaggaata tataatcaat
     1141 gatattaatg aaaaactaat gaatttgtac aaggttataa gagatgactg cgaaggattg
     1201 ataagtgaac tttatagact gcaagaagaa tatataatgt tagaagactt ggtaaagaaa
     1261 gaaaaatttt attatgaatg tagagaacag tttaatttaa atcaagtaag cgatacaaaa
     1321 atgagtgcat tacttatttt tttaaataag acatgtttta atggtctata tagagaaaat
     1381 tcacagggtt tatttaatgt gccttttggg aaaagaaatt ctcctcaaat ttttgaagaa
     1441 aaaaatataa aagaaatatc aagacttttg aatataaaaa ataaagaagg aaagtataga
     1501 gttcatatat taaataaatc atttgaggat ttagatgatt atattgataa taataccttt
     1561 gtctattttg atcctccata cagacccgtt acttttggcg ggtttaattc atacaataag
     1621 agtagtttta atgatgaaag tcagataaaa ctaagagatt tttttgttac acaagacaaa
     1681 aagggcgcaa ggttaatgct tagtaactca gatcctaaag tgcttgacaa aaatgataat
     1741 ttttttgatg acttatataa agattttaca ataaaaagag tgactgcaaa tcgaatgatt
     1801 aacagtaaag gttctggaag aggtcctatt aatgaattat taataatgaa ttatgattga
     1861 gggggtagta gaagagcatg aagtatgaaa aaaatacgca aacttttgat tatttaatgt
     1921 caacgttaaa agaatctatt aaggattgag gttattttgt taattgggac aaggtttata
     1981 ataatgttaa ggacatagaa attgaattaa atatattaaa ttatttaata ggaaaggaca
     2041 atatagagga tgaagcaaaa tatttaataa aaaagcatcc agaagtgatg aaagttattc
     2101 caattctatt agcatgtaga gaaaaagaat atcagataac ttttactgat tctaataatc
     2161 gatttgttaa taaaagttat aaatttaata ataaaactat cactgatgaa gatattaatg
     2221 atgctgtaga atttatgaaa aaaacaaaga tatttgattt gttttctaat aaaactatta
     2281 aaaatgttgt agattatgtt tttggtgttg aagttggctt agatagcaat ggaaggaaaa
     2341 atcgttcagg agattcaatg gaaggtattg ttgaaaagta catatgcgag ttatgcaaag
     2401 aaaaaagata tgaatatctt aagcaagcga ctcctaatca aattaaaatg gcatgggggt
     2461 atcatgtaac agtggataag acagcaagaa gatttgattt tgcaataaat actggtaata
     2521 atttatattt gattgaaaca aattattacg gtgggggtgg ctctaaatta aaagctacag
     2581 caggtgaata caagactttg tatgatataa ccaaaaaaga tggccatacg tttatatgga
     2641 taacagatgg taaaggatgg ttaactgcca gtaaaccact agaagaaaca ttttatcata
     2701 atgagtacat atttaactta tctatgctgg aagataatat attgtatgaa attgtaaaat
     2761 aagaataata taaactgtaa cgaatacaat atccttaaaa tggtaataat aagatatctt
     2821 tatctttagg aggttcaaag tgttagaaac ttataggcct agagaagata tcttacaatc
     2881 gactacgttg tggagttttc ctgacagagg agattgggca acgcataata acaaatacag
     2941 aggtaattgg tctccgtata ttccaagaaa catcattaaa agatatagta aagttggaga
     3001 catggttttg gatccatttg ttggaggcgg caccacactt attgaagcta aactcttgaa
     3061 tagaaatgca ataggaatag atataaatcc agaagcaatt aaaattacaa aacaaaattt
     3121 aaattttaaa tataaaacat catcaagaat ttatttatta tgcagaaatg ctaataattt
     3181 agattttatt aaaaacaata gtatcgattt aatatgtacc catccaccat acgctaatat
     3241 gataaaatat agcaaaaaca ttgaagggga tatatcttta tgtagtcctg atgatttttt
     3301 gatgcagtta aaaattgttg cgcatgaatt atatagagtt ttaaaggcaa ataagtattg
     3361 tgctttttta atgggtgata ttcgaaagaa taaaaatatc attcctttag gttttatgtc
     3421 aatgaaagta tttcaggaaa ctggattttt attgaaagag atcgtaatta aagaacaaca
     3481 taattgtaaa tcaactccta aatggataag taaaagcagt caatataatt ttttacttat
     3541 agcacatgaa tacttattta tatttcaaaa accagatcct tcaatttgtt tggacaagag
     3601 tgtacttaag taatagaata gtatttctat tttcctcaat aaaggcaaag aagaaaatta
     3661 ttataaagtc aggcgggttt taatgacaaa agtaataatc attgacgaca ttcgaatcga
     3721 ggtagttaaa aagaaaataa aaaatatcta tttatacgta catcccccaa acggtaaagt
     3781 taaaatttct gctccattaa agatgaagga cgaagctatt cgtaattttg tcatttcaaa
     3841 acttgcctgg attaaaaagc agcaagctaa atttcaagct caagagggac aagctgaaac
     3901 agagtatgtc tcaggagaaa gacattatta tcaagggatt ccctacatac taaatgtcat
     3961 gcatacgaat aaaaaacaga gagtggaaat aagtaaaaaa caaattaact tatttattcg
     4021 tccagatagc actaaagagc agcgagagcg catattattg gaatggtacc gatctgaact
     4081 taaaaaacaa atcccaggtt taatttccaa atgggaaaat atcattggtg taaaagtaaa
     4141 ctcatggggc gttaaattga tgaaaacaag atgggggact tgtaatccta aagctaagcg
     4201 gatatggatt aacctggaac tggcaaagaa aagtccaaga tgtttagagt atattatcgt
     4261 tcatgagatg gttcatttac ttgaaaagca tcataataaa agattttatg cttttatgaa
     4321 acagttctta ccaaattgga gagagattaa agctgagctg aacggcttaa aattggaaag
     4381 ccctaaataa ggttattgtc atcaaaagtg aaagaccttt tgcacttgct gcagaaggct
     4441 tttttattaa aatttgtttt ttaaatgtta taataattat taagtaaaag atacatattt
     4501 taattggagg cataatgatg aaaatttgtt caaatgaaga tatatcaaag gagaaagtaa
     4561 tggaattatt taaacttcat tggggaagtc cgatgatggt tatttcaact ggtgtatata
     4621 attgcagtga attagatgct tttgctgctg tcaatgagga aggggaaacc attggtttaa
     4681 ttacttatct aatcaaagat aatgaatgtg aaataatatc tttagaaggg ggagatggca
     4741 tacccattag ggatgaaatt gagttgataa aatggattta gatagctatt tatctttcgt
     4801 atagaggcca tttgcttgtc ctttaattaa gtgttaaaat gatatataaa tattgagaaa
     4861 agaggtaaga gtgtatgtgg gttctttttg caatactatc ggcggttttt gcggcactga
     4921 cttccatact ggcgaaaatc ggtattgaag gtgtaaattc taatctggct acagcggtaa
     4981 gaaccgttgt tgttgtgatt atggcatggt taatggtatt tatcacggat gcacagggtg
     5041 gtttgtcggc aatcagcaaa aagagctgga tattccttat tttatccggg cttgcgacag
     5101 gggcatcctg gatgtgttat tataaagctt tgcagactgg ggaagcttca aaggtcgtgc
     5161 cgattgataa attgagtgtg gtgataacag ttgttcttgc ctttattttt cttcatgaga
     5221 aagtggatgc caaaacgatt attggctgta ttatgattac agcaggtact ttggttatgg
     5281 tcttataaaa ttaggctccc atcacctgat atattttctg gtggtaagga gcctaatatg
     5341 ttggttaaga gttatatttg aggtgttcta tcaaaattat tatattacga tatttttggt
     5401 aaccaagttt tatcaatatt tttcccagct tacaatctct gaaagctcca ttcttttctt
     5461 gctttcagga gctgtatctg caggatagcc tattggaagc agtgcaacaa cttccaggtt
     5521 ttcaggcagt tctaaagcct tatggcactg cttagcatca aaagcacata cccaacaggt
     5581 gcccagacct aaatctgttg cggccagggt catatgatcg atagcaatgg ctacatcgat
     5641 gtcgcagtga tctttgccat catttctttt ccatgatttt gaatggtcac cgcatacgac
     5701 aattaccgca ggaactgttt taaaccaatc tcttggatag gtttcagcga ttttattttt
     5761 tattatctta tcggtgacta caataaagtg ccagggctgt ttgttaacag cagagggagc
     5821 aattcgggct gcttcaagaa cttgaagaag cttttctttt tcaacgggtt tgtctaaata
     5881 acttcttacg gaatatcttt ttttagcaag ttcaagaaat gacataaatg attcctcctt
     5941 aggggatttt tatttatgag attatttttt aattaataaa tatggactat aaaaaatata
     6001 taataaatta aaaactgtgt caaaaatatg attccaacag aaaaatattt tattccatat
     6061 atagcatctt atgttaaggt atgatattat agagcatatt gtggttgtaa ttaagcgggt
     6121 tattaaatgc ttaaagatca ttgtgctgta aataagaaat gaaataaaga cgtgaagaat
     6181 ataagtgatg agaaagttta atgaaatatt tttcctgggt aaaggtttaa gcttctaata
     6241 aaaagttgtt taaaattttc tttacaatca tagaaagtta atttaggggg gaagcttatg
     6301 gatatcatgt tagagatact taagtttatt caatcttttt caaatccttt ttttgatttt
     6361 ctttttgaat tggttacgat gttaggggag gattatttct ttattctggt taccgggctt
     6421 tttttctggt gctttgataa gtattttggg tataagctgg cctatattta tctcacgggt
     6481 ggaaccgtca atacgatgat taaagaaatt gttaaattcc caaggccaat aggctatgag
     6541 ggtatccgtt ccctcagggt agggacggca ggcggttatt cttttcccag tggtcatacc
     6601 cagcaggcta cttctttatt taccactttg atgataaatt ttaagaagaa atggctttat
     6661 cttataggta caggagcaat gcttttggtt ggtttttcaa ggatgtattt aggtgtccac
     6721 tggccaacag atgttgctgg aggtttactg attggtcttg tgtggacgta tcttgccatt
     6781 aaagtatttg attggtcaaa ggataaagaa aatcctgtac ttcttgggat tcttgttata
     6841 ccgatgttta tatgtctttt gttttttaaa acagctactt actataaagc cattggtacc
     6901 ctgactgccc tgtggattgg ttacatcatt gaagataaat atatccatta tgaaacaaaa
     6961 gcagtctgtt ggaagcaagt gataaaaggt attattggat ttttaggact tattgtgatt
     7021 aaagtctttg taaaaaaact tttaccagaa aatatatata gcgattttgt aagatatgca
     7081 tttatgggct tttggatgac gataatatct ccactacttt ttagaatatc taatcttgag
     7141 gaaagaggag cattggggaa accaaaaagc tatacggaag ttacttttac aaaagagctt
     7201 aattcttaat ggtattataa taaaaaaccg tggttttcat ttaccacggt tttttattta
     7261 acttatttaa tggcttccac ttcatagccc agcgcttcta attgggctac gatacctgta
     7321 tcgcctatca tatgtcctga gccgacaacg acaaaatagc tcttgtcatt gcctgaatct
     7381 aaatattgtt ttatcgcctc agtcatatta ttatttctga gagtaaatgt tttttccgtg
     7441 aattcttctt ttgtttcttc gtctccttca atggaattaa aatatgattt acagaaagct
     7501 tcggtatccc cttcaatcca ttgattgagc catgtatcta atatttcagc actttcattt
     7561 tttaatgcag gatcttcaaa gccttttagt gtttctgcta attgtttttc ctgatattct
     7621 ggtgaaaaac tgtccagcat atcagcctga tattttatgc tttccagttc cagaattggt
     7681 ttattgctat ataatgcatt aataatgaaa tagatatcaa ttcccagggt tcctgaataa
     7741 ggtgaacttc caacaccacc ttcttttgtt accagaagct gggacagttg ttgtgataat
     7801 gcccatggtt tcagatacat gtattgctcc tttttcattc cgatatttgc catcacgttg
     7861 tctaatttag cagcagtttc ttctgagata taatcataaa tagtcgttcc atccgggcat
     7921 tgcatttttt cctgcaaata aaggattcct tcctggtcag atataatatt ggcttctact
     7981 gctaaagcat cagaattttt aaaggcttct tttattttag tactcatggg ataaatatca
     8041 tgttttgcga catggacaga accaagcaga taaaccgttg tatcgtcatc agatactttc
     8101 cagaagaaac ctttagagcc tacattcgct tctttacaaa gagtttctac aagtcttgtc
     8161 gcaaagagcg ctgcttcttc aagagtacat actttatcca agttataatt gttgccatct
     8221 ccgcgaagga cagacttttg aagcatgaaa tcgatggcag cttttctgtg gcttgctgtt
     8281 tcgggtacgg tttcagccaa ttcatattta aggacttgat ggtacaagcc ttcaagaact
     8341 gtttctctgg tgatattatt gtctgcttca taattgttaa aagaacctga agctggaaat
     8401 cctaacttgg acagtttatt atcgacgttg tttaatagag tatttagggc tgcctggtca
     8461 acaggagcca gcatattgtc ataccattcg atagggataa gctcaaggcg ttctccttca
     8521 aataaggttg gggccgccca ggaatgataa gtaatggctt tttgtgtcag ttggtcattc
     8581 gcatagagcg gtagccaaaa ttgtgaaatg atgctaaaga acaataaaaa acttaaaatt
     8641 tttcttgtaa acttattttt catgtgtaat cccctttctt aaatttatgt atatttaaaa
     8701 taataaaata atttggctta taattctttt ttgagataga tacgcagtga aagaaaatat
     8761 gaaaaactta cccataggat aaagacaatt cccaatggaa ttaattgatg agaaagaaaa
     8821 tctttaatta ttccccattc tgcattaaac ataaaaagtg gtacaaaaaa gcctattaaa
     8881 aagtaaagag gaagtgactt ttcaatcata aaacttaaca gaatccaaag ggaatggatc
     8941 attagaccta taaaagcaat aaccatattt tgccttagat tgcctatatt aaatctgata
     9001 ccggcagaag aaaaaattaa atataaaaga taattaaaag cgtagcatac aggaagtgtc
     9061 agcaaagtta ataaaaaagt gcttcttacg atttcctttc ttgtgacagg aaggctgtta
     9121 atcaattttg cactgctatt tttatggtca tactccctga ttgtaacatt aattacaaag
     9181 tttatcaaga atgcaaaccc taacagatta gtaatgtctg accattgcaa aagcagaaga
     9241 tatatattca gggcaatcca ggaacaatac tgttttctgg gcagcaataa aattcttttc
     9301 ataagcactt gcatatttac ccttctttct ataaatcttt attggtataa agtttattgg
     9361 ttaataaata agatatgaga aataaagcaa aggatattat aaaaaagtaa tagatattgt
     9421 gagggttatt acataatttt tttatctgta agatcaataa cagccaggga tttatggaat
     9481 ctactgttat atcaatgggc aggactagaa taacaaaaat actggttaat aaaatggata
     9541 tattggcaac aaggccccaa agggctttta ttaaaaaact gattggtatt gttaacgctg
     9601 tcgtagccgt aagcatggcg atgattagca taagatccgt taaactggca aaattcataa
     9661 tggaaagccc tgtttttttt aatatggcac ctgtaatgcc cgtcaaaaga aaactgatta
     9721 agcccatgat aattacaaaa gcgtatttgg cacggatttg ctctgttctt ttaatactca
     9781 ggctatgtat taaaaaatcg tgttttgcat ctaagtattg ggcacaggct ataagaataa
     9841 gaatatataa accgctgcaa aggtatggat aaaaaataga tttaccagtg gatgatgtaa
     9901 ttaaggcttc caataggata aaatgaatta aataaggaat aattaataag caccagattt
     9961 tacgagtgat ataaaaatct ttaaggataa ttttccaggt cgttttattc attaccagcc
    10021 tgcctccttg ccatatgcat atgaaccatg atatcttgca gggaaggtct ttcaatgata
    10081 accgaatctc caaattcctg ccggatttta gggatatcca tagtcaaggc ttcaaaacca
    10141 tagggagatt gtgtgcaatg aataaaggat ttttttattt cttcattcag taattcattt
    10201 gctcctttaa cgatgccgta gctttctgaa atttcgtctt ttgtacggct aaacaggatt
    10261 tccccgtcat atataaaagt gatataatca gcaattttat ctaaatcact ggttatatgg
    10321 cttgagaaaa agatggattt attttcgttt tgcattaatt ccaaaagcag ttccaataat
    10381 tcagcacgaa ataccgggtc cagacctgcg gttggttcat ccataataat caggtcagga
    10441 tcatgggaga gggccatagc aatagaaaat ttcattttca ttccttttga gagttttttt
    10501 aattttttat ctaagggcag ttcaaattga cggatatatt tattaaataa atcatcattc
    10561 cattttgaat aaaaaggggc aatgattctt ttaatttcat acagtttgaa ttcttcataa
    10621 taacagttgt catcataaac aaaaccaatc ctgtctttta tttctttttc atttttaatg
    10681 tggtcttttc caaatacttt aatactgccg ctgtcttttt tgataagatt cataattaac
    10741 ttaataactg tggtttttcc tgcaccgtta ggtccaataa aacccataat atagccttct
    10801 tttaattcaa aagaaacatt ttttaaagaa aaatctttat agcttttatt gatattttga
    10861 acttccaaga tattattcat gtttatcctc ctcatataga aatctcaaaa tttctatcaa
    10921 gtcatctagg gtcatgttaa gaagtttact gtctttaatg gcttctgaaa gtttgttttc
    10981 aatttctgcc agtttctttt cccgcattaa ttctttgttt tgactggcta caaaagaacc
    11041 ttttcctgca accgtttcaa tgagtttttc tctttccagt tcttcataag ctcttttggt
    11101 tgtaatcacg ctaatgcgaa gctcctgtgc cagtttacgg atcgaaggca gttgagtgcc
    11161 ttcagacagt tccccatata atatttggct tcttatttgc tcaacaattt gttcgtagat
    11221 aggtttgtct gagctattgg acaaaataat tttcatcatt atcacctatt ctaaatgtat
    11281 atactgtata taatatatat gaacagtata tacgttatac acgcaatgtc aagcagcaat
    11341 aaaaactttt taaatttttg tgtgggaaat gttggacaat ctgttgaagc acaggtgcat
    11401 ggagccctgg aagatatgga aaaccggctg aaagaaattg gtttaacatt agagtctgtg
    11461 gttaaagtag atgtattatt aagagatcca tgggatatac ctgttatgga aaaggtgttc
    11521 agagaacgtt ttgcaggtaa atatcctgca agaaaaacca tacaaacaga gtttgcccat
    11581 gttggcggac cagatggact gcatgtccaa attgatacca ttgcatataa aaagaaataa
    11641 aacaggcaga tcaagtttta taatagagcc tgaaagcatc ctttttgatt tagtctgaaa
    11701 ggatgctttt ttgcatattt ttatagactt cattttcgaa ctatttaaat tttttggaca
    11761 gaaggtaatt aattgagtag aataataatt aatggaataa tttataaaat cataaaggga
    11821 tacaaaaaat gtaatggggt taaaacttga cagtaaaatg gtcgtaaata tgatgaataa
    11881 gaggaatatt gcttttttat gggtatgatt aaaggaggta aataatgata ggtgcaatta
    11941 ttggagatat tataggttcc cgttttgaat ttaataataa aaaaacgaaa gactttgaat
    12001 tatttgcgaa agaatgcaga ccgaccgatg atagtctgat gaccctggcg attgcaaaag
    12061 ccattatgga agcagaaaaa atcatcaggg attcaaggga tgaagattat tatacacaca
    12121 tagaaaaaat gactgttttg tgtatgcagg aactggggcg taaatatcct gatgccggtt
    12181 ttggcgggat gttttttaaa tggcttttta gtgataatcc aatgccatac aacagttttg
    12241 ggaatggtgc ggcgatgcgg ataagcccgg ttggctttgc ggcaaaaaca gaagaagaag
    12301 cctgcaagct ctcaaaaatc gtaacgggtg taacccataa tcataaggaa ggtttgaaag
    12361 gagccgaagc agtagctgta gccatttata tggcacgcca tggggcaggc aaagacgaaa
    12421 ttaagaaaaa aatagaagaa aattattatg atttagattt cagattagat gatatcaggc
    12481 cttcatatca atttgatgtg acttgtcaag ggtctgtacc acaagccatt aaagcatttt
    12541 tagagtccat ttcttttgaa gatgctattc gtaatgctgt ttcattaggc ggagacagtg
    12601 ataccatagg ggcgattaca ggaggaatag cagaggcgta ttatggagtg cctggagaat
    12661 ttaaagaaaa ggccctttct tatctgacgg aggatctgcg attaattgtt aaggaatggt
    12721 atgatttcat agcataaaag acaaagaggt gtaaatggtg aaaatcataa aagtgacagc
    12781 aggtgtaata gtggataagg gtaaggtttt aattacaagg agggcaccag aagattgttt
    12841 agctggaggc tgggaattcc caggaggtaa gattgaggca aacgaaacgc ctgaagaatg
    12901 tcttgtaaga gaactaaaag aagagcttaa tattaatgtt tctgttgatc aattttgtac
    12961 agaagtaagt cactcagatg gagacaggca gattaattta atggcctttt actgtacaat
    13021 tttagatgga gaaattcaga tatctgtcca tgacaaattc aaatggattc cagtgaatga
    13081 cctattaaaa tacgatttgt taccagcaga tgttctgatt gctaaaaaag ttatagaaga
    13141 attcaatgct taaaaagatt aaacaggggc ttttaagatt tttgctttat cagtcatttc
    13201 aatcaaaacc ggcagtacga cttcttttgt atgaccatct tgtcaaaaac tttaagcgaa
    13261 acaaggggac ggttccgtgt ttcattaaat cgtaaaaaat gtcactgata atccagataa
    13321 ataagtaaga aataagggat tgatatgctg catatgataa aagaaaattt tattaaatcc
    13381 attggaagac ttttcagact gctattattg gtgttgtttt ttatatttgt cttagcagct
    13441 ttgctatatt ttgttacagg taaaatttat tttaactttg tccttctttt ttcggtactt
    13501 tttgcaatgg caataagtat ttcttttctg gaacataaag attttttcag ggtggggctg
    13561 gcgtttcgtg ccagggatat cgtttgtggt atgattgctt ttgctggaac ggctgttttt
    13621 tatgccatat ttattgtcat ggaaagtttt attaacggta agaacttatt cccccagtat
    13681 cttgaccgta ttacaggaca ttttagccgt tcctttttaa gtgtcttgct gattcccctt
    13741 acagaggaga tgctgcaccg aggctatatt ttgggaaact gtttccaaag tttaaagtgt
    13801 tggcagcgca gtttactttc agcatttatt ttttcattaa cccattgggt ctacatgcca
    13861 gggacagata tgtattactt tttacttgtt aatgttgttt ctacctttgt tcttggcttt
    13921 ttttaaacaa tgtccgccgg ataacaggct caatctggac aggtgctgcc atccactggt
    13981 tatataatta ttttggtata agtatcttcg gcagtacacc tccacttaga tttattgctt
    14041 ttagcgatat catttgactt gttttatttt tctttttcga gaaaataagc agggggccat
    14101 tttggagtgc ctgtaatacg ccttataata attaatggca aaggggacct gaatacatcg
    14161 ggcttatgag ttcagttgat gcattgaaga aaaatgtaga attattggag aatatcatca
    14221 tcttactctt tcctttactt aaataacgtg ctataatggt aaagcgattt taattaaatt
    14281 ggaggtataa ataatgaata aattaaatat caagaaaata atggcactgt ttgtgtcact
    14341 aactttaatt tttgctttgg cggcatgtgg tttaaaagga aaaggtgatt taagctatgt
    14401 taagaataaa gggactttaa ttattggtta tacgaactat gcgcccatga attattacga
    14461 tgaagatgga aactttactg gttttgatac agagcttgcg attttggttt gtgaaaagct
    14521 tggggttcaa cctgaatttg tagaaatcaa ctgggatacc aaagaagtag agcttaatgc
    14581 aaaatctatt gactgtattt ggaatggttt gaccattgat gatgaacgta aagcgactat
    14641 ggaaattact aagccttatg ttaagaacgc ccaggtggtt ttaatgaagg aaggcgcaag
    14701 ttatacgggt acagaatctt taattggaaa gactgttgtg gctgaacaag gttctgctgg
    14761 tgaaaagacc atcttagaag acgcaaactt aagtcaggca aactatgtgc ctaaaatact
    14821 tcaaaccgac tgtttgatgg aattaaaagc tggaaccgtt gatgcagcag tattggattt
    14881 aactttggca aaaaccatga cgggtgaagg taccagctat caggatattg taattgttga
    14941 tgaattagca gaagaatact acggggttgc tttccgtaag ggttctgata tttgtgaaga
    15001 agtcaataaa atttttgatg aattccttgc agatggtatc atggaagcat tggctgaaaa
    15061 atatggtttg gaattagcag aataagctat acgtaaagat ataaaatatt gtaaaatgaa
    15121 gaaaagtcag agggtaacct ctgactcact acttagttga gggaagataa ctatgaatga
    15181 gatttctgta attattagcc gtttatcgga aagttttttg cttaattgta aactttttgc
    15241 tttaacctta atattttctc tacctttagg gcttctcatt gcttttggtt caatgagccg
    15301 tttccgtccg cttcgctggc tggtacggac ttttgtctgg ataatccggg gaacgccttt
    15361 gatgctgcag cttattatca tgttctttgg cccagggctt ttgggcttgt ctttttcctg
    15421 gcctggtggg gcatcaggac gttttattgc tgcaacggtg gctttcatta ttaactatgc
    15481 gtgttatttt tcagaaattt accgtggggg cattgagagc attcctaagg ggcaatatga
    15541 agcaggtcag gttctgggta tgacaaagcc ccaaatcttt ttccatgtaa ccctgcttca
    15601 agtgataaag cgtattatcc ctcctgttgg taatgaggtt atgaccttag taaaagatac
    15661 atcccttgcc cgcattattg ctaataaaga aattattatg atggctggtg aatataccaa
    15721 caagggactg atttggcctc tttttgcgac aggtatttat ttccttgtct ttataggggt
    15781 cctgactata ctctttaact ggctggaaaa gaaactaagc tatttccgta cataagatcc
    15841 gtacataaga aaggagggaa cccattggca attttagaag ttaaggggat tgaaaagtct
    15901 ttcgaagata ctcatgtatt aaaaaatatt aatttttctt tggaaaaagg cgaagtgatg
    15961 gccattattg gctcctcggg aagcggaaag actacacttt taagatgtct taatttttta
    16021 gagcgaccag ataaaggaag cattattgta aacaatgaag tcatttttga tgcaacaaat
    16081 cccagtacac aaaaggaaag tgaactgagg aaaaagcgcc tccattttgg tctggttttt
    16141 caaaacttca acctttttcc tcaatatacc gttttaaaaa atgttaccct ggcagcagaa
    16201 cttctggcaa aagaacggcc agattataaa caaaataaaa aagcaattac agaagaaatt
    16261 caagaacggg ctaaaatgct cctggctcag gttggtttat cagataagct ggatttttat
    16321 cctcatatga tatctggcgg gcagcagcag cgtgtagcca ttgcccgtgc tttagcctta
    16381 gaacctgaca ttttatgttt tgatgaaccg acatctgctt tagaccctga acttacaggg
    16441 gaagtactaa aagtaattaa aaatttagca gagcgggata cgaccatgat tattgtaacc
    16501 catgagatgg attttgcccg ggatgtggca gaccgggtaa tctttatgga tgatggtgta
    16561 attgttgaac agggttcgcc tcaagaagtc tttggaaatc caaaagaaga gagaacgaaa
    16621 cagttcttag cccgctataa taaaacttaa cttaaataag ccaggaaagt attttgattt
    16681 ttaaagacat gctggttaat gacatgagaa tgcaatatga tagcattctc tttttatata
    16741 tgacatagtt aattattgaa ggataacagt aaaaatttta tgccctattg tttaagcgat
    16801 ttaatcaagt attgtctttg taatctgaaa acaccctcat tgaaatgatc agaagaaggc
    16861 taaaagttca ggagagtgtt ggtgaaaagg tcgtacttga gaaaatccat tatcgcatag
    16921 atttcagaag ataatgttaa agattcagga ggagagtgta atgggtgtta aaattttaat
    16981 ttcgcttatt gcaatgatac ttatatacat gattgattat atcaaaagaa aggaaatgaa
    17041 agaagtagtt attagatatt cgaaatttgg cagattgcag tttaaaaaga atctatttag
    17101 tgataatgag aacgacctga taagttataa tcaaaagatt cgctttggaa aatctaatcc
    17161 tacaattcag ttaaaagact atcaaaaaga aaagctctat gtatatttgc atgctttaaa
    17221 ttatattcat gaaatacaaa acgaaattgt agaaaatatg tgtaaagaag cgcttgatta
    17281 ttgccagcgt tggtttaagc tcgatgaaga cggtaatccg gtccgttatg aatacatacg
    17341 gaaaagtttt gatgtggata ccattatact aaagattgaa aacagtgttg ttatggcaat
    17401 tataagggga ttttgtgatg ggttgattga tgatcaggat attgtcgtgt atatcaattg
    17461 tactgataaa gaaattgtaa aatattcaat tgaagaataa agataatttt tggtgaggtt
    17521 taatttatgg agaaaaaaga agaagcatta atagacattc caaattttgg ggtattaaga
    17581 tttttatgat acctttatgg aactttaagt gttagtgaga gagatttaac ctatgaacat
    17641 gttaagtttg gaaagcatga gcccaaaata attataaaag attataaaga agcaatgttt
    17701 gatttatatc aaaagacttt atcttgttta tttgagcttc aaaatgaaat tacagaaaat
    17761 ctctatcagg aaactataaa atattgtgat gaagagggca tacgtaatag cagtgataat
    17821 cctatcattt atgtaacgga ccatttcata attgatgaga taatgattaa tacgatagac
    17881 ggacagataa tgctaacggt ttggggaaga gcttataatg aatatggaac aatttattcg
    17941 aatccgatga agatttatat atcaatgcat atgtatatta tccaaagaaa ttcatcatgt
    18001 atgacagatc atattatgac gggtaaaaaa agttagtcaa agtgtgtaaa tgatacaata
    18061 aagagaaata gttatctatt aaacattaaa aagtttaaat tatctttcta ttctaaaaag
    18121 ataacataag attgaaattt gggaaagtat tagatataat gaagcagtaa ttttgtgata
    18181 attttaaaaa atgatttttt gtagggggag attaataagt atggagttta tcaccgaagt
    18241 acctattagt acaaaaggaa aggctgcacg ttttatggtg acagggtaga atttgacgga
    18301 aatacggttt tgtacaggga cattgaaatt ttagaaacaa cagcaaaggt tattattagt
    18361 aggttcttag gaattccagt gggacgaaaa tttgaagctt atgtaaggtt tagaatgaaa
    18421 gacggtaaaa aatataatat caatatgaat gcgatggcga cttttggaat ttcttttgga
    18481 agaaatcccc gtcaatacga agaactttat ccaaaagtat ttgaagctgt ctatgctatt
    18541 gtggcaaaag cgatggctca accctatatt aataagataa gatctggagc cacagttgaa
    18601 gttgcgggtc taatgattaa cgctacagca gcgaaaccag taaagtccag aaaagatatt
    18661 gtaattaaca gtagcaacta cagggaagct gtgatttcgc aaggttatgg tgttacggtg
    18721 tacaataagc aaggggatgt attatgggat tctccttatt ttaactataa aaatatttta
    18781 ctgattccac atattttaaa tgaaattttt gcataagcat gaggtgatga tatggcaaaa
    18841 gttaaatgtc acatttgtaa gaaaagcaat acagattcag tatgcagtcg ctgtggggcc
    18901 catattggtg atactgcttc agaaaggtta gtacttgcta caagtggagc aattttatat
    18961 aacgacgatg ctgggaaata tgtggataag ttttcatatt gcaaggtggc tatgaccgac
    19021 cgacgtttaa ttatttacaa tatcaagcca gattcagata acccagcatg gtggtttttc
    19081 agatctataa aaaacctttt tgaaaaattt ccctgcattt cgattaatct taatgatatt
    19141 gagcaagtgc gccgctatcg tgagtggttt gcagtaaagt ccaaaacggg cacttattcc
    19201 atatgtgtat caaaacagaa ggagttggat cagttattta ctccatataa ggatccggaa
    19261 gaacaatagt tattttttta ataatatact gtttgatgac aagaatttat atttcacttt
    19321 aactttttgg cttgttttga tga
//
LOCUS       DAOSUD010000041        19150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_1835_length_19150_cov_25.533752, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000041 DAOSUD010000000
VERSION     DAOSUD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 19150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19150
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_19150_cov_25.533752"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1146
                     /locus_tag="PLG49_06615"
     CDS             151..1146
                     /locus_tag="PLG49_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HOQ17032.1"
                     /translation="MLEKNYFFMGFSFTSIIMIVIFILFNTSFLEKVRIYENKDEAYE
                     SINKYIIDDVYYNANHYFNCVGSNEELDIEILKQAIEKNSSPPFKMKPVEMIKYIKVK
                     RIKHFIGKTPLYTESDLYMQELIIELIASYDLINEMYSYYENNEYEQDDFAAAKELHQ
                     ELIYKINKLSDLLEKFNLAYHKEYNEKIIRCLEEYKSNGHLSNYYAYRCLNSANEIRR
                     YMIEKGFSPERNDYILGFDEEEFSNLCKAFRTACKEYERYINDSESIKSESNFVFISS
                     PELSQSLQNMEDILPLIPEITVEHEMYESNRKSAIDDMFFDYYELAVSEYYKIFE"
     gene            complement(1148..1777)
                     /locus_tag="PLG49_06620"
     CDS             complement(1148..1777)
                     /locus_tag="PLG49_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOQ17033.1"
                     /translation="MSNRIVLPEYMKIAVDMAARIYKGEFDEQEKLRGRSTLAGEYNV
                     SPETIRRAMRLLEDMEVVTVNQGSGIYVKSKEKAYRFMERFKQKESIDELKVGIKKLV
                     EEKKRIEEEIEAKINTIIDYADRLKHSNLITPIEITIPKNSHVIGKNIIDTNFWQNTG
                     ATIVGIKRKDELIVSPGPYTEFKEGDILIIVGDPFIKSRTDEFLSEQSP"
     gene            2325..3098
                     /locus_tag="PLG49_06625"
     CDS             2325..3098
                     /locus_tag="PLG49_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ17034.1"
                     /translation="MKKIMSIVLMAMMILTLAGCGKGEGESAAPGQGTSEGKKVIVMG
                     TNAEFPPFEYREGEKVVGFDVEIAKKIAEKLGAELEIEDMSFDGLIPALEAGKIDFIV
                     AGMSITEDRKKNVDFSKGYYTASQVIITLKDNTEIKGPEDLKNKTIGVQLGTTGDEEA
                     QKVEGAQVIQFNAAYAAIMDLQNGKLDAVVLDYEPARNFASQNDKIQILPVELTQEEY
                     AIAVKKGNKELVDAINAVLEEMKESGEYDSLVNEYFAKE"
     gene            3200..3868
                     /locus_tag="PLG49_06630"
     CDS             3200..3868
                     /locus_tag="PLG49_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOQ17035.1"
                     /translation="MLEGLKFSLLVTAFAAVIGIIIGLVVALLRITEFKPFKFLKGKK
                     YLGRLADFNPFSFVATIYVDIIRGTPSVVQLMIMHTVIFGSVKNVPKLVVAGLAFGIN
                     SGAYVAEIIRAGIQGLDKGQMEAARSLGMSYSMAMYYIIIPQAIKNILPALVSEFIIL
                     LKETSIVGFIGGMDLLRSANIIISQTYRAFEPLMAVALIYLILTGIFTKIMRIVERRM
                     RVSD"
     gene            3861..4583
                     /locus_tag="PLG49_06635"
     CDS             3861..4583
                     /locus_tag="PLG49_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ17036.1"
                     /translation="MIEVKNLYKSFGEIEVLKGIDEHIMKEEVVVIIGPSGSGKSTFL
                     RCINLMEQPTQGTIKIDGVDITSPKTNINQVRQKVGMVFQQFNLFPHMNVMDNITLAP
                     MKLKGLTKNEAEEVAYTLLDKVGLREKAMEYPNKLSGGQKQRIAIARALAMNPEIMLF
                     DEPTSALDPEMVKEVLEVIRELANEGMTMMIVTHEMGFAKEVGTRLLFMDEGKIIERG
                     NPKEVFANPQEERTRIFLSKVL"
     gene            4745..5281
                     /gene="raiA"
                     /locus_tag="PLG49_06640"
     CDS             4745..5281
                     /gene="raiA"
                     /locus_tag="PLG49_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOQ17037.1"
                     /translation="MRYIISGTNIEITNALQQRVMDKLSKLEKFFVADTEAQVTLSVE
                     KLRHIIEVTIPFQGTILRAEVDDKDMYAAIDEVVDVLERQLLKHKNRLKDKHRSNSPF
                     KKEFIKDIESTENDDEKIVIEKIKRFAVKPMDVEEAAMQMDLLGHNFFVFRNADTDEV
                     NVVYKRKNGTYGLIEPEF"
     gene            5478..8054
                     /gene="secA"
                     /locus_tag="PLG49_06645"
     CDS             5478..8054
                     /gene="secA"
                     /locus_tag="PLG49_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ17038.1"
                     /translation="MKGFLEKIFGTHSEREIRKIEGIVDEIEALGPKMEQLSDAQLRA
                     KTDEFKSRLAQGETLDDILPEAFAVVREASTRVLGMRHFRVQLIGGIILHQGRIAEMK
                     TGEGKTLVATLPAYLNALEGKGVHIVTVNDYLANRDAQWMGKIYEFLGLSVGVILNEL
                     DSEERRKAYNCDITYGTNNEFGFDYLRDNMVIYKEEMVQRELHYAIIDEVDSVLIDEA
                     RTPLIISGSSSKSTHLYKAADMFVRRLKKGRVLTEESGLDRLLQQEIKEEGDFVVDEK
                     AKTVTLTAEGVKKAEQYFSLENLADPENMEIQHHINLALKAHNIMYLDKDYVIKDGEI
                     VIVDEFTGRLMPGRRYSDGLHQAIEAKENVEVKRESKTLATITFQNYFNKYKKKAGMT
                     GTALTEEEEFRHIYGMDVVVIPTNKPVIRVDHPDVVYKTAKAKFEAVVNEIEESYKKG
                     QPVLVGTITIDTSEMLSQMLKKRGIKHQVLNAKYHEKEAEIVALAGQKGAVTIATNMA
                     GRGTDIKLGEGVKELGGLKIIGTERHEARRIDNQLRGRSGRQGDPGESRFYISLEDDL
                     MRLFGSERILKMFDAMGLPEDQPIEHKMLTKAIENAQKKVEGNNFSIRKHLLEYDRVM
                     NEQREIIYGERRKVLEGDNLKDNILNMIKNIINEVVEVHTGESTHPEEWDLTALNQHV
                     RKIIPIRDIHISKEEVEKLTKDILKEKLVDEAIKLYNKKEEEIGSEQMREIERVILLR
                     VIDQKWMDHIDNMEQMRQGIGLRAYGQRDPLVEYQFIGYEMFEEMSANIQEETIRALY
                     NVKVAYKPERERVAEPIATNKGDDTGVRTPVKRKEQKVGRNDPCPCGSGKKYKHCCGK
                     NA"
     gene            8085..9186
                     /gene="prfB"
                     /locus_tag="PLG49_06650"
     CDS             join(8085..8159,8161..9186)
                     /gene="prfB"
                     /locus_tag="PLG49_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144428561.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOQ17039.1"
                     /translation="MLELEQLKQDLQPYRKYLQEMGASLDISGAKTKLGELEEKSARI
                     DFWDNMDEAQKILQEIKSLKSKIEKFEELYREYEDLITLIEIGLEEQDEGLISEVTQE
                     AKQFINHYEEMRIATLLDGEYDRSNAILTLHAGAGGTEACDWVSMLFRMYTRWAEDKG
                     YKVETLDYLPGDEAGIKSVTIQISGENAYGYLKSEKGVHRLVRISPFDASGRRHTSFA
                     SCDVLPEIDDDVDIEIKPEDIRIDTYRSSGAGGQHVNTTDSAVRITHIPTNIVVQCQN
                     ERSQHKNRETAMKMLRAKLYEKKLEEQMEKLQDIRGEVKEIGWGSQIRSYVFHPYNMV
                     KDHRTNEETGNVSAVMDGDIDRFINAYLKWIH"
     gene            9220..9684
                     /locus_tag="PLG49_06655"
     CDS             9220..9684
                     /locus_tag="PLG49_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOQ17040.1"
                     /translation="MATRQEVIFKLGNEEYGLDIMKVYGIERYQKITKVPNTPEYIEG
                     IINLRGEVYPIYNLRKKFHMPEKAVDDETKMIIVNSNEMMVGFIVDSVVEIRHIDEDN
                     IKPAPKLISGIDRRYIIGVGEVDDRMIIILDVDLILEDEEKETVSQIINQAE"
     gene            9857..10300
                     /locus_tag="PLG49_06660"
     CDS             9857..10300
                     /locus_tag="PLG49_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ17041.1"
                     /translation="MKRETVFFIVDKNVLPEVFIKVVEAKRLLESGKVQTVQEATDLV
                     GISRSSFYKYKDSVFPFYENSRGRTITISIQLEDRTGLLSHILNVIASVGANILTINQ
                     TIPVNRIANVTITMETNPTKDDIKEIIDKVESIDGVLNLKILARE"
     gene            10309..11547
                     /locus_tag="PLG49_06665"
     CDS             10309..11547
                     /locus_tag="PLG49_06665"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOQ17042.1"
                     /translation="MIHIAVLGYGTVGSGVVEVINRNRDSINKKAGKEINIKYVLDLR
                     DFPGDPVEKILTHDIEDILNDEEVRIVIEVMGGIEPAYTYVKRALLSGKSVVTSNKEL
                     VAKHGTELLKIARERDINFLFEASVGGGIPLIRPLNQSLTADEIYEITGILNGTTNYI
                     LTKMTEEGKSFDEVLKRAQEKGYAERDPRADVEGHDACRKIAILSSLAFGMEVNYEDI
                     YTEGITKITEKDILYAEKIKHSIKLLATSKKIDHRVFARVSPVLISNDHPLAMVKGVF
                     NAVLVKGNVVGDVMFYGKGAGKFPTASAVVADVVDAVKHLNRNIVFSWSTEKMDLMEL
                     KDVPVRYFVRIKYEDFENAKNEVEQVFGTKEIIVLENLKDEFAFISNEETEGILQEKI
                     DILKKEKSIKGIENIIRVEY"
     gene            11573..12796
                     /locus_tag="PLG49_06670"
     CDS             11573..12796
                     /locus_tag="PLG49_06670"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOQ17043.1"
                     /translation="MKYVVILGDGMADEPIKELGNKTPLQYANKPMMDYMAKHGEVGM
                     VYTIPEGMVPGSDTANLSVMGYNPETYYSGRSPLEAVSMGVKLLDSDVTFRCNLVTLS
                     EEGDYDEKIILDHSSDEISTEEARELIKAVNEHFKNSEIEFFPGISYRHLMLWHEGPD
                     DFDLTPPHDILGQKITDYLPKGKQKDIIYKMMKESYNFLQNHPININRKKCGLKAANS
                     IWIWGQGRKPLLPSFEEKYGLKGSVISAVDLIKGIGICAGLDSIDVEGATGNIHTNFT
                     GKAMAAVAELKKGKDFVYVHLEAPDECGHRNELDNKVKSIELIDEKIIKIIKEELDAA
                     GEEYKMLILPDHPTPIRLRTHTADPVPYLIYQNTKEKLNPIQSYDEISALKSNHCFLK
                     GHELMNYFLKCKEDE"
     gene            12798..14012
                     /locus_tag="PLG49_06675"
     CDS             12798..14012
                     /locus_tag="PLG49_06675"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOQ17044.1"
                     /translation="MLIVQKYGGSSLATVERVFNIANRIINTYKQGHKVVVVLSAQGK
                     TTDQLIAKAHEIDPNPSKREMDMLISTGEQQSVALMAMAINKLGYPAVSLNAFQVGMH
                     TNSTYSNARLKKIDTERIEAELDRKNIVLVTGFQGINKFDDITTLGRSGSDTTAVAIA
                     AALNADKCEIYTDVDGVYTADPRIVKNARKLDEITYDEMLELAALGAKVLHHRSVEIA
                     KKYNVELEVRSSFNDSEGTIVREVCNVEKMLVSGVAGDKNVARISVIGIPDTPGKAFE
                     LFSLLAKNKISVDIILQSIGRDGTKDISFTVARSDLHDALKIIKENLERLGAKDVVYK
                     EDVAKVSIVGAGMATNPGIAAMMFEAMYNAGINISMISTSEVKISVLIDEKYTEEAMN
                     AIHERFRLAMER"
     gene            complement(14131..15864)
                     /locus_tag="PLG49_06680"
     CDS             complement(14131..15864)
                     /locus_tag="PLG49_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17045.1"
                     /translation="MKIKLKALLSYYKPYKWLFIADMICAFIASGVGLLFPLIVRYLT
                     ENVFNTNTSIAAGLIIKILSLMLFLTLIEYLCNYFITSYGHVMGALMEHDMRNDIFSH
                     LQKLSFTYYDNQKVGQIMSRITNDLLDITELCHHGPEDILISLTKLIGAFIILANIDI
                     KLTLIVFAFIPFMLLFAYYYNNKMNKAFKKNREKIAAINAAIEDSLSGIRVVKSFANE
                     DIELEKFKQNNIQFVRSKRDSYHYIAKYYSGINAFGSLIYVVLIIGASLFISNNQMNT
                     GDLVTFLLYIGSFLEPVKKLVNFAEQFQNGITGFERFHEILSIHPDIEDKKDAIEIKN
                     IKGNIRFENVSFHYHGTSDAVFSNINFNVKAGEYIALVGPSGVGKTTLCSLIPRFYEV
                     SSGSIKIDNIDIRDIQLKSLRQNIGIVQQDVYLFAGTVMDNIRYGKHDATDEEIIAAA
                     KNANAHDFIMALPNGYQTDIGQRGIKLSGGQKQRISIARVFLKNPPILIFDEATSALD
                     NESEKVVQESLEKLARNRTTFVIAHRLSTIKNAQRIVVLTEEGITEEGTHEELLALNG
                     LYAQLYNMQFQ"
     gene            16102..18243
                     /locus_tag="PLG49_06685"
     CDS             16102..18243
                     /locus_tag="PLG49_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOQ17046.1"
                     /translation="MDIVKKLQEEFNLKTFQVENTIKLIDEGNTIPFIARYRKEATGS
                     LNDVVLRNFYERLNYLRNLQEKKEQITRAISEQGKLTEELTQAIEKAETLTELEDIYR
                     PFRPKRRTRATIAKEKGLEPLAQMIWEQELTNPVEEIAKDYIDLEKEVTSVEDAINGA
                     MDIIAEWISDNADYRKYIRKETFYRGILVVKARDEKEESVYEMYYDYSEAVKEIPGHR
                     ILAINRGEKEKFLSVKIDAPVDRIILFLEKQIIKTEGPAHELLKLVIEDSYKRLIAPA
                     IEREIRNELTQKAETGALEVFRSNLSQLLMQPPIKGKVVLGLDPAYRTGCKIAVIDET
                     GKVLDITVIYPTPPQNNIEESKAIIIDLINKYNVDIVSIGNGTASRESEMFIADVIKE
                     LEKTVHYIIVNEAGASVYSASPLASEEFPEYDVGIRSAISIGRRLQDPLAELVKIDPK
                     SIGVGQYQHDMNQKRLEESLQGVVEDCVNRVGVDLNTASVSLLQYVSGITSAVAKNIV
                     EYREANGKFKNRKELLKVKKLGPKVFEQCAGFLRIPESDNIFDNTGVHPESYDVAQRL
                     LDRIGLHLNDIKENKVKNLRKNIQDIDALAQSLEVGVPTLEDIIKELEKPGRDPREDM
                     PKPILRSDVLKLEDLKENMVLTGTVRNITDFGAFVDIGVHQDGLVHISQMSDKYIKHP
                     LEVVKVGEIVKVAVLSVDINKKRIALTMKGI"
     gene            18260..19144
                     /locus_tag="PLG49_06690"
     CDS             18260..19144
                     /locus_tag="PLG49_06690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17047.1"
                     /translation="MEVRSFNDDYHEVGQLVINYLHHEYDSVEEMKDNWKGKNLILKV
                     AEDNGKVIGFGALDLVRKKGEPNSDLYGIVVDPAYRRQGVGTLLMKSLVDDAKGLGIR
                     YIYSTLDRNNEEGINFLIHCGFEYKRSVFMMEMNEPVINEIECPEGIEIKEMSVLENV
                     EEFLEVYEEIFGHINDKEQLTEKISKENNYNFFLMYRVSKSTGTKKIIGFCIVENIDE
                     DGINRRIDILGVIDKYARRGLGKVLYITVLNHYWSVPETKRITISLKSSSENVKKFYL
                     SCGMEETYIQETYRYEIL"
BASE COUNT         6862 a         2637 c         3920 g         5731 t
ORIGIN      
        1 taagaattta aaataattat caaattttga gattataaaa attaataata tattaattaa
       61 acttaatttt cttttattaa ttctcgtatt ttattaattt aaaattataa gtcagatggg
      121 taattatttg aatggaaaaa gagggaaaat atgcttgaaa agaattattt ttttatggga
      181 ttttctttta cttcaatcat tatgatcgtt atatttattc tttttaatac atctttttta
      241 gaaaaagtaa ggatttacga aaataaagat gaggcctatg agagtattaa taaatatata
      301 atagatgatg tttattacaa tgcaaatcac tattttaact gcgttggcag taatgaagaa
      361 ttggatatag aaattcttaa gcaagctatt gagaaaaaca gttctcctcc ttttaaaatg
      421 aaacccgtag aaatgataaa atatattaaa gtaaaaagaa tcaagcattt tattgggaag
      481 acccctttat atacggaatc agacctgtat atgcaagaac ttattattga gcttatagcc
      541 agttatgatc ttatcaatga aatgtatagc tattatgaaa acaatgaata tgaacaggat
      601 gattttgctg ctgcaaaaga gttacaccag gaattaattt ataagatcaa caagctctct
      661 gatttacttg aaaaatttaa tctggcttat cataaagaat ataacgaaaa aataatcaga
      721 tgtcttgagg aatataaaag taacggtcat ttatctaact attatgctta cagatgttta
      781 aacagtgcca atgagattag aagatatatg atagaaaaag gttttagtcc tgaacgaaat
      841 gattatattt tgggctttga tgaagaagag ttttcaaacc tgtgcaaagc gttcaggaca
      901 gcttgtaaag aatacgagcg atatataaat gattctgaat cgataaaaag tgaaagcaat
      961 tttgttttta tatcttcacc tgaattaagc cagagccttc aaaacatgga agatattctt
     1021 ccattgattc ctgaaataac agttgaacat gagatgtatg aatcaaatag gaagtcggct
     1081 atagatgata tgttttttga ttattatgaa ctggctgtca gcgaatatta taaaattttt
     1141 gagtaactta tggtgattgt tctgataaaa attcatccgt cctgctttta atgaaggggt
     1201 ctccaacaat aattaatata tcgccttctt taaactctgt gtaaggacct ggtgagacaa
     1261 tcagttcgtc ttttcttttt atgcctacaa tggttgcacc ggtattttgc cagaagtttg
     1321 tatcaataat atttttccca ataacatgag agttttttgg aatagtgatt tcaatagggg
     1381 tgataagatt gctgtgtttt aaccggtcag cataatcgat aattgtgtta atttttgctt
     1441 caatttcttc ttcgattctt ttcttttctt ctacgagttt tttaatgcca acttttagct
     1501 catcaatgct ttctttttgc ttaaagcgtt ccatgaaccg ataagctttt tcttttgatt
     1561 tgacatatat accacttccc tggtttacgg ttacgacctc catatcttca agaagtctca
     1621 tagctcttct tatggtttca ggtgacacat tatattcccc tgctaaggta gaacgacctc
     1681 ttaatttttc ttgttcgtca aattcgccct tatatattct tgctgccata tccacagcta
     1741 ttttcatata ttccgggagt acaattctat tactcatagg attcacctct gataagtgat
     1801 ataaatctat aatagtattt tatcatatag aagtattttt gtaacaaaag aatagaataa
     1861 ttacagaaaa gatattattt ctaaaattta ataagttatt taataattat gcaagaataa
     1921 gttaagaatt atttacaata ttaattaata atcatactca ttcgttaaaa cgtttattaa
     1981 ttatgttatt ataataaaag aaactacttt acataatagt aactgataaa gaacgataca
     2041 catagttatc cacatgatat tttactgagt tttactttat gcacaaagtt atcaacatta
     2101 tccacagctc ttatgttcac aaaacatctg ttttgtggaa attacttaat ttatccacaa
     2161 aatgagaatg ttgaattttt caataataat taattctcct ttttaaaata gcggttgctt
     2221 aaaaaaagat aattaaaaaa ttaaacaaaa agttgttgac tatttattca ttgcaatgta
     2281 taattattca aaaataaatt tttatttgta aggggagaag aactatgaaa aaaataatga
     2341 gtatcgtttt aatggctatg atgatattga ctttagcagg atgtggaaaa ggagaaggag
     2401 aaagtgctgc tccaggtcaa ggtacttcag aaggtaagaa agttattgtg atgggaacca
     2461 atgcagaatt tccaccattt gagtatagag aaggagaaaa agtagtaggc tttgatgttg
     2521 aaattgcaaa aaaaatagca gaaaaattag gggctgaatt agaaattgaa gatatgtcat
     2581 ttgatggctt gatacctgcc ctggaagctg gtaaaattga ttttatagta gcaggtatga
     2641 gtattacgga agaccgaaag aaaaatgttg acttttctaa aggctattat acagcaagtc
     2701 aggtgattat tacattaaaa gataatacag aaatcaaagg tcctgaagac ttaaagaata
     2761 agacaattgg ggtacaatta ggaacaacag gcgatgaaga agctcaaaaa gtagaaggtg
     2821 cgcaggtgat tcaatttaat gctgcttatg cagccattat ggatttacaa aatggtaaat
     2881 tagatgctgt agtattagat tatgaacctg ccagaaattt tgcttctcag aatgacaaaa
     2941 ttcaaattct tcctgtagaa ctgactcagg aagagtatgc gattgctgtt aaaaagggaa
     3001 ataaagaatt ggttgatgcc ataaatgctg ttttggaaga aatgaaagaa agcggagaat
     3061 atgatagttt agtcaatgaa tattttgcaa aggagtagcc tttattggct gactcctttc
     3121 taaatgaaat caattcaaag aaggggtgaa gcctgtgtca gtatgggaga ttttttttgg
     3181 taataacaga tatcagtaca tgttagaagg tttgaaattt tcattactgg taacagcatt
     3241 tgcagcagtc ataggaatta taataggttt agttgttgca cttttaagga taacagaatt
     3301 taaacctttc aaatttctga aaggaaagaa atatcttggc agattggcag attttaatcc
     3361 tttttcattt gtagcaacca tttatgtgga tattattcga ggaacccctt cggttgttca
     3421 attgatgatt atgcacaccg ttatttttgg cagtgtcaaa aatgtaccca agctagtggt
     3481 ggctggtttg gcctttggca ttaatagcgg tgcatatgtt gccgaaataa taagggcagg
     3541 gatacaagga cttgataagg ggcaaatgga agcagcccgt tctcttggaa tgagttacag
     3601 tatggccatg tattatatta ttattcctca ggcaatcaaa aatatcctgc ctgccctggt
     3661 tagtgaattt attatcttat taaaagaaac ctccattgta ggatttattg ggggaatgga
     3721 tttacttcgt tctgctaata ttattataag ccagacctac agagcatttg aacctttaat
     3781 ggctgttgcc cttatttatc tgattttgac aggaatcttc acgaagatta tgcgcattgt
     3841 agaaaggagg atgagggtta gtgattgagg taaaaaattt atataaaagt tttggagaga
     3901 tagaagtatt aaaaggtatc gatgaacaca taatgaaaga agaggttgtt gttataatag
     3961 gtccaagtgg ttcaggaaaa tctacctttc tgcgatgcat caatttaatg gaacagccta
     4021 cccagggaac aataaaaata gatggtgtag acattacatc ccctaaaacc aatattaatc
     4081 aggtccgtca aaaagtaggg atggtttttc aacagtttaa tttatttccc catatgaatg
     4141 tgatggataa tatcactttg gctcctatga agttaaaagg attaactaaa aatgaggcag
     4201 aagaagttgc ctatacatta ttagataagg ttgggcttag ggaaaaagca atggaatacc
     4261 ccaataaatt gtcaggaggt caaaagcaga gaatagctat tgcaagagct ttagccatga
     4321 atccggaaat aatgcttttt gacgaaccta cgtcagcatt agatcctgag atggtgaaag
     4381 aagtgttgga ggttatcagg gaactggcga atgaaggcat gaccatgatg attgtaactc
     4441 acgaaatggg ctttgcaaag gaagttggga caagattatt atttatggat gaaggaaaaa
     4501 ttatagaaag aggaaatcca aaagaagtat ttgctaatcc acaggaagaa aggactcgta
     4561 tttttttaag caaagtatta taaatgtgtc agagaataag ataggttttt tgataaaata
     4621 aaaaataatt cccaacttta aaaattaaaa atcaaaaaag gattttgata taatttgtag
     4681 aatataatat aatataaaag attaagcttt tttgaaaagt actattaaga aggagttgac
     4741 catcatgcgt tatattatta gtgggacgaa tatagagata accaacgcac tccagcaaag
     4801 ggtaatggac aagcttagta aattagaaaa gttttttgta gcagatactg aagcccaggt
     4861 taccctgagt gttgagaagt taagacatat aattgaagtt accattccat ttcaaggtac
     4921 aattctaaga gcagaagttg atgataagga tatgtatgca gccattgatg aagtggttga
     4981 tgttttagaa agacaattat taaagcataa aaacagactg aaagataagc atagaagcaa
     5041 tagccccttt aagaaagaat ttataaaaga cattgaaagt actgaaaatg atgatgaaaa
     5101 aattgttatc gaaaaaatta aacgttttgc agtaaaacca atggatgttg aagaagcagc
     5161 aatgcaaatg gatttattag gccataactt ctttgttttt agaaatgcag atacagatga
     5221 agtaaatgtt gtatataaac gtaaaaatgg cacatatgga ttaatagaac ctgagtttta
     5281 atcataaaaa ccctcactaa gtgagggttt ttattgttag ccaaatatat ccattaattg
     5341 attgtagtat tgtaaatcct tataatgttt gttatactaa acattaaagt gcctctttgt
     5401 gcactttatc ttttggtttc agacctgttt tatgaatttg ttatttttga atatatatca
     5461 aaaaagaagg tgaatttgtg aaaggttttt tagaaaaaat atttggaacc catagtgaaa
     5521 gagaaattag aaaaatagaa ggaattgttg atgaaattga agctttgggc cctaaaatgg
     5581 agcaactgtc agatgctcag ttaagggcca aaacagatga atttaaaagt agactggctc
     5641 agggtgaaac cctcgatgat attttaccag aggcttttgc tgtggttagg gaagcttcta
     5701 caagagttct tggcatgaga cactttaggg ttcaactgat tggtggcatt attcttcacc
     5761 agggaagaat tgctgaaatg aaaacagggg aaggtaaaac cttagtggct acccttccgg
     5821 cttatcttaa tgcccttgaa ggtaagggtg ttcatattgt gacagtaaac gattatttgg
     5881 ctaaccgtga cgctcaatgg atgggtaaaa tttatgaatt tttaggttta tccgtaggtg
     5941 ttattttaaa tgaattggat tcagaggaaa gacgcaaagc atataactgc gatattactt
     6001 atggaaccaa taatgaattt ggctttgact atttaaggga caacatggtt atttataaag
     6061 aagaaatggt tcaaagagag cttcattatg cgattattga cgaagtggac tctgtattaa
     6121 ttgacgaagc cagaacccct cttattatat ctggaagcag ttctaaatca acgcatttat
     6181 ataaggcggc ggatatgttt gtccgcaggc tgaaaaaagg tagggtatta actgaagaat
     6241 caggcctgga cagattatta cagcaagaga taaaagaaga gggagatttt gttgttgatg
     6301 aaaaggcaaa gaccgttaca ttgacagcag aaggggttaa aaaggctgaa caatatttta
     6361 gtcttgaaaa cttagcagac cctgaaaata tggagataca gcatcatatt aatctggctc
     6421 ttaaagctca taatattatg tatttagata aagactatgt gattaaagat ggagaaattg
     6481 taatcgtcga tgaatttacc ggccgtctta tgcctggaag aaggtattca gatggtttgc
     6541 atcaggcgat agaagcaaaa gagaatgtag aagttaaaag agaaagtaaa accctcgcaa
     6601 cgattacttt ccagaattat tttaacaagt ataagaaaaa agcggggatg acaggtactg
     6661 ctttaacaga agaagaagaa ttcagacata tttatgggat ggacgttgtt gtcattccaa
     6721 cgaataaacc ggttatacgt gttgatcatc ccgatgtggt atataagact gccaaagcaa
     6781 aatttgaggc agtggttaac gaaatagaag aatcttataa aaaaggtcag cctgttcttg
     6841 tgggtaccat taccattgat acctcagaaa tgcttagtca aatgcttaaa aaacgaggta
     6901 tcaaacacca ggtattaaat gccaaatatc atgaaaaaga agcagaaatt gtagcccttg
     6961 ccggacaaaa aggtgcagtg accatagcga caaacatggc aggacgtggt acggatatta
     7021 aacttggtga aggcgttaag gaattaggtg gcttaaagat tattggtaca gaaagacatg
     7081 aagcaagacg tattgataat cagttaagag gacgttccgg tcgtcaggga gacccgggag
     7141 aatcccgttt ctatatttct cttgaagatg atttaatgag gctttttggt tctgaaagaa
     7201 tactaaaaat gtttgatgct atgggccttc cagaagacca gcctatagaa cataaaatgc
     7261 ttacaaaggc catagaaaat gcacagaaga aagtagaagg caataacttc tcaattcgta
     7321 agcatttgct cgaatatgac agagttatga atgaacaaag agaaattata tatggagaaa
     7381 gaagaaaagt attagaaggg gataacttaa aagataatat cttaaatatg attaaaaaca
     7441 taatcaatga agtcgttgaa gttcatacag gagaaagtac tcatcctgaa gaatgggatt
     7501 taactgcttt aaatcaacat gttagaaaaa ttatccccat aagagatatt catatttcaa
     7561 aagaagaagt agaaaaattg accaaagata ttctcaaaga aaagctggta gacgaagcca
     7621 ttaagcttta taataaaaag gaagaagaaa ttggatcaga acaaatgcgc gaaattgagc
     7681 gggtaattct gctcagggtt attgaccaaa agtggatgga ccatatcgat aatatggaac
     7741 agatgcgcca gggtattggt cttcgtgctt atggacaaag agatccttta gtagaatatc
     7801 agtttatagg ttacgaaatg tttgaagaaa tgagtgccaa cattcaggaa gaaactatcc
     7861 gggcattata taatgttaaa gtagcctata aacctgaaag agagagagtg gctgaaccta
     7921 tagccaccaa taaaggagac gatacgggag taagaactcc tgtaaagaga aaagaacaaa
     7981 aagtaggacg aaatgatcct tgtccttgtg gaagcggtaa aaaatataaa cattgctgtg
     8041 gtaagaatgc atagttataa agtaaatata aaggagatga tcacttgtta gagttggaac
     8101 aattaaaaca agatcttcaa ccctatcgta aatatttgca ggaaatgggg gcttctcttt
     8161 gacatttccg gggctaaaac taaacttgga gaattagaag aaaaatctgc cagaatagat
     8221 ttttgggaca atatggatga agcccagaaa atacttcagg aaataaaaag tctaaagtct
     8281 aaaatagaaa agttcgaaga attatataga gagtatgaag atttaattac tttaatagaa
     8341 attggattag aagagcagga tgaagggcta atttctgaag taacgcaaga agcaaagcaa
     8401 tttattaatc attacgaaga aatgagaatt gctactttat tagatggaga atatgacagg
     8461 agtaatgcca tccttaccct tcatgccggc gctggaggaa cagaggcatg tgattgggtg
     8521 tcgatgcttt tcaggatgta tacacgatgg gctgaagata aaggctataa agtagaaact
     8581 ctggattacc tccctggtga tgaagcaggc attaagtctg tgaccattca aataagcgga
     8641 gaaaatgctt atggttactt aaaatctgaa aaaggtgtcc atcgtttagt taggatttct
     8701 ccttttgatg cctctggaag aagacatacc tcttttgcct catgcgatgt tcttcctgaa
     8761 atcgatgatg atgtagatat agaaattaaa ccagaagata ttcggataga tacttacagg
     8821 tccagtggag caggcggaca acatgtaaat accactgatt cagctgtacg tatcacccat
     8881 atccctacta atattgttgt tcaatgccaa aatgaaaggt ctcagcataa aaacagagaa
     8941 accgctatga aaatgttaag ggcaaaatta tatgaaaaga agttggaaga gcaaatggaa
     9001 aaacttcagg acatccgggg ggaagtaaaa gaaattggct ggggaagcca gataagatct
     9061 tatgtttttc atccttataa tatggtaaaa gatcatcgta ccaatgaaga aacaggaaat
     9121 gtttccgccg taatggatgg agatattgat agatttatta atgcttatct taaatggatc
     9181 cactgatttt aatgtttaat tttgagggga ggaaaagcca tggcaacaag gcaagaagta
     9241 atttttaaat taggaaacga agaatatggc ttggatatta tgaaagtcta tggcattgag
     9301 aggtatcaaa aaataacaaa ggttcctaat acgccagagt atattgaagg aattatcaat
     9361 ttaagaggag aggtttatcc catttataac ctaagaaaaa aatttcatat gcccgaaaaa
     9421 gcggtggatg atgagaccaa gatgatcatc gtaaattcca atgagatgat ggttggcttt
     9481 attgttgatt ctgtagtgga aatcaggcat atagatgaag acaatataaa acctgcacct
     9541 aagcttattt caggaataga cagacgatat attataggtg tcggggaagt cgatgacaga
     9601 atgattatta tattggatgt agatttaatt ttagaagacg aagaaaaaga gaccgtctct
     9661 caaatcataa atcaggctga ataagcactt ttaacaaaaa acgcgaagat attcgcgttt
     9721 ttttattatt tttttttgta ttattttctt gcattccatg cataaatatg atatattcct
     9781 tgtatcaaga acaaatgtct ttttctcaaa tacaaaaagc atcgttttgt tcttttgctt
     9841 aaaaaggagg ttgagcatga agcgcgagac cgtatttttt atcgttgata agaatgtact
     9901 tccagaggta tttataaaag ttgttgaagc caagagacta ttagagtctg gcaaagtgca
     9961 aaccgttcag gaagctactg acctggtggg aattagcaga agttcgtttt ataaatacaa
    10021 agactcggta tttccttttt acgaaaattc ccgggggaga acgattacca tatccattca
    10081 attagaagac agaacgggtc tgttatctca tatattaaat gttatagctt ctgtaggggc
    10141 aaatatatta accatcaatc aaactatacc agttaataga attgccaatg ttaccattac
    10201 catggaaact aatccgacaa aggatgatat aaaagaaatc atagataaag tagaatccat
    10261 agatggcgtt ttaaacttaa aaatattagc tagggagtga tttaaaagat gattcatatt
    10321 gcggtgctgg gttatggaac agtagggtct ggggttgtag aagtcattaa caggaacagg
    10381 gacagtatta ataaaaaagc agggaaagag attaatatta agtatgtact agatttaagg
    10441 gattttccag gggacccggt agaaaaaatt ttaactcatg atattgaaga tattttaaat
    10501 gatgaagaag taagaattgt tattgaagtt atgggtggta ttgagccagc ttatacatat
    10561 gttaaaaggg ccttattaag tggcaagagt gttgtaacat ctaataagga actcgtagcc
    10621 aagcacggca cagagctttt aaaaattgcc cgtgaaagag acattaattt cttatttgaa
    10681 gccagtgtag ggggtggtat acctctgata cgtcctttaa atcaatcctt aacggctgat
    10741 gaaatctatg aaattactgg gattttaaac ggaaccacca attacatctt aaccaaaatg
    10801 actgaagaag gtaaaagttt tgatgaagtc ctgaaaagag ctcaggaaaa aggttatgct
    10861 gaaagagatc caagagccga tgtagaaggt catgatgctt gcagaaaaat agctattttg
    10921 tcttctctgg ctttcggtat ggaagttaat tatgaagata tctatacgga aggaattaca
    10981 aaaattacag aaaaagatat tctttatgct gaaaaaatta aacattcaat taagctttta
    11041 gcgacaagta aaaaaattga ccatagggtt tttgccagag tatcaccagt tttgataagt
    11101 aatgaccatc ctttggcaat ggtaaaaggt gtttttaatg ctgtattggt caagggaaat
    11161 gttgtaggtg atgtgatgtt ttatggaaaa ggtgcaggga aatttcctac agccagtgca
    11221 gtagtagcgg atgtcgtaga tgctgtaaaa catttaaacc gcaacattgt attttcatgg
    11281 agcacagaaa agatggattt aatggaatta aaagatgttc ctgtgcgtta ttttgtcagg
    11341 attaaatacg aggactttga aaatgccaaa aatgaagtag aacaagtatt tggcactaaa
    11401 gaaatcattg ttttagagaa tttgaaggat gagtttgcat ttatttctaa tgaagaaaca
    11461 gaaggcatcc ttcaggaaaa aattgacatt ttgaagaaag aaaaatcaat caaaggcata
    11521 gaaaatatta ttagggtaga atattaaatt tataagtaga ggagaatcac ttgtgaaata
    11581 cgtagtaatt cttggagatg gaatggcaga tgagcccata aaagaacttg gtaataagac
    11641 tcctcttcaa tatgcgaata agccaatgat ggattatatg gcgaaacatg gagaagtagg
    11701 aatggtctat acgataccag aggggatggt ccctggtagc gatacagcca atttatctgt
    11761 aatgggatat aatcctgaaa cttattacag cggaaggtca cccctggagg ccgtgagtat
    11821 gggtgtaaaa cttttagaca gcgatgttac tttccgttgt aatttagtaa ccctttctga
    11881 agaaggggat tatgatgaaa aaattatttt agaccacagt tcggatgaga tttcaaccga
    11941 agaagccaga gaattaataa aagcagttaa tgaacatttt aaaaacagcg aaattgagtt
    12001 tttcccaggt ataagctaca ggcatttaat gttatggcat gaggggcctg atgattttga
    12061 tttaacccct ccccatgata ttctggggca aaagattaca gattatcttc ctaaaggaaa
    12121 acaaaaagat attatttaca agatgatgaa ggaaagttat aattttttgc agaaccatcc
    12181 cataaatata aatagaaaga aatgtggctt aaaagctgcg aattctattt ggatctgggg
    12241 acaaggaaga aagccgttat taccttcttt tgaagaaaaa tacggcttaa aaggttctgt
    12301 tatatcagca gtcgatttaa tcaaaggaat tggaatatgt gctggacttg attcgattga
    12361 cgtagaaggt gcgacaggca acattcatac caatttcacc ggtaaagcta tggcggcagt
    12421 ggctgaactt aaaaagggta aagattttgt atatgttcac ctggaagcac ctgatgagtg
    12481 tggtcataga aatgaattag ataataaggt aaaatctatt gagttaattg atgagaaaat
    12541 cattaaaatc attaaagaag aattagacgc agcaggggaa gaatataaaa tgctgatttt
    12601 gccagaccac ccaacgccaa tcagactcag aacccatact gctgatcctg taccttattt
    12661 gatttatcaa aatacgaagg aaaaattaaa tcctatacag tcttatgatg aaatatctgc
    12721 tttaaaaagc aatcattgct ttttaaaagg tcatgaatta atgaattatt ttctaaagtg
    12781 taaggaggac gaataacttg ttaatcgtac aaaaatatgg tggaagctca ttagcgacag
    12841 tagaaagagt ttttaatatc gcaaatagaa ttattaatac ttataagcaa gggcacaaag
    12901 tggttgtagt cttatctgct cagggaaaga caacggacca gttaatcgct aaagcccatg
    12961 aaattgatcc aaatccttca aaaagggaaa tggatatgtt aatttctaca ggggaacaac
    13021 aatctgtagc tcttatggca atggccatta ataaattagg ctatcctgct gtttccctta
    13081 atgcattcca ggttggtatg cacaccaata gtacctatag caatgccaga ttaaagaaga
    13141 ttgatacaga aagaattgaa gcagaactgg ataggaaaaa tatagtctta gtaacaggat
    13201 tccagggaat caataagttc gatgatatta caaccctggg cagaagtggt tcagatacga
    13261 ctgctgtagc aattgcagct gctttaaacg ctgacaaatg tgaaatatat acagatgttg
    13321 acggtgttta tacagcagac cccagaatcg ttaaaaatgc cagaaagtta gatgaaatta
    13381 cttacgatga aatgttagaa ttagctgccc tcggtgcaaa ggtattgcat catcgttctg
    13441 ttgagattgc aaagaaatat aatgtggaat tagaggtaag atccagcttt aatgattcag
    13501 aaggtacaat tgttagggag gtatgtaatg tggaaaaaat gttggtaagt ggagttgctg
    13561 gggataaaaa tgtagcaagg atttcagtaa taggtatacc cgatactcct ggcaaggcat
    13621 ttgaactttt ttcattactg gcaaaaaata aaattagtgt agatattata ttacaatcta
    13681 taggtcgtga cggcacaaaa gatatttcct ttacagtagc cagaagtgat ttacatgatg
    13741 ccttaaagat tataaaagaa aatcttgaaa gattgggtgc aaaagacgtt gtatacaaag
    13801 aagatgtagc aaaagtatca atcgttggag caggaatggc aaccaatcct ggaattgctg
    13861 ccatgatgtt tgaagcaatg tacaatgcag gaattaatat cagcatgatt tctacatcag
    13921 aggttaagat ttctgtactt attgatgaaa aatatacaga agaagctatg aatgcaatcc
    13981 atgaacgttt cagattagct atggaaagat aataaaaaaa gggctgtgca ttaatctaat
    14041 gttaatgtga cagcctttac tttttgttat tataaaagga actgttcata aataaattta
    14101 tgtaacagtc cttctagcgg gcttatgaat ttattgaaat tgcatattat atagctgggc
    14161 atacaaacca tttaaagcca gcaattcttc gtgagtgcct tcttctgtaa tgccctcttc
    14221 cgtaagaacc acaattcttt gagcattttt tatggttgat aatctgtggg caatgacaaa
    14281 agtcgttctg tttctggcta gtttttctaa tgattcctgg accacttttt cgctttcatt
    14341 atctaaggcc gaagtagctt catcaaaaat gagaatggga ggatttttta agaataccct
    14401 ggcaatgctg attctttgtt tttgccctcc ggaaagctta ataccacgct gaccgatatc
    14461 tgtctgatag ccattgggca atgccataat aaagtcatgg gcattggcat ttttggccgc
    14521 agcaataatt tcttcgtctg tggcatcatg cttgccgtaa cggatattat ccattacagt
    14581 acctgcaaac aaatagacat cttgctgaac aattccaata ttttgcctta aggattttaa
    14641 ttggatatcc cttatatcga tattatcgat ttttatggaa ccgctggaaa cctcataaaa
    14701 tcttgggatc agactgcata aagtagtttt tccaacacct gaaggtccca ccagggcaat
    14761 atattctccg gctttgacat taaaattaat gtttgaaaaa acagcatccg atgttccgtg
    14821 ataatggaag ctgacatttt caaaacggat atttccttta atatttttaa tttctatggc
    14881 atctttttta tcttctatat ccggatggat ggataggatt tcatggaatc tttcgaagcc
    14941 agtaattcca ttttgaaatt gctctgcaaa gtttacgagt ttttttacgg gttctaagaa
    15001 acttccgatg tataataaga aagtgactaa atccccggta ttcatttgat tattgctaat
    15061 aaacaatgaa gcaccaataa ttaagacaac ataaattaac gaaccaaagg cattgattcc
    15121 actgtaatat tttgctatat agtggtagct gtcacgttta ctgcggacaa attgaatatt
    15181 gttttgctta aatttttcta actcaatatc ttcattagca aaagatttta ctacccgtat
    15241 tccagaaaga ctgtcttcta tggcagcatt gatggcagca attttttccc ggttcttttt
    15301 aaaggcttta ttcattttat tattatagta gtaggcaaat aaaagcataa aaggaataaa
    15361 ggcaaaaaca attaaggtta attttatatc gatgtttgcc agaataataa aggcacctat
    15421 cagtttagtt aaagaaatca aaatatcttc aggtccatga tggcaaagtt ctgtaatatc
    15481 aagcaggtca ttagtgattc tggacataat ctgtccaact ttttgattgt cataataggt
    15541 aaaagacagt ttttgtagat gtgagaaaat atcattacgc atatcgtgtt ccattagggc
    15601 acccatgaca tggccgtagg atgtaatgaa ataattgcaa agatattcaa tcagggttaa
    15661 gaaaagcatc agggataata tttttatgat gagccctgct gctatggatg tatttgtgtt
    15721 gaatacattt tcggttaagt acctgacaat cagaggaaag agcaggccaa cccctgaagc
    15781 aataaaggca caaatcatat ctgcaataaa taaccattta tacggtttat aataagataa
    15841 taaggcttta agcttaattt tcatatcagg tctccttttc tttctcaaaa gctttatgag
    15901 tgtttaaaca tttaattttc ttaatagata tggatttgct ggcatattta tttttagaat
    15961 gatagcattt taatgtttta ttgtcaataa agatgtagaa agctgtatat agcttataag
    16021 gatttaatat tgtttgaaga tagcaaatcg tatataatag agttaatgta aaaatgaaaa
    16081 ctagaaatga atggtggtta aatggatata gtaaaaaaac ttcaggaaga atttaatctg
    16141 aaaacttttc aggttgaaaa tacaatcaag ctgattgatg aagggaacac aattcctttt
    16201 attgccagat atcgcaaaga agcaacaggc tcattaaatg atgtggtttt aagaaatttt
    16261 tacgagaggt taaattatct gcgtaacctg caagaaaaga aagaacaaat cacaagggct
    16321 atcagtgaac aaggtaagct tacggaggag cttacacaag caattgaaaa agctgaaacc
    16381 ttaacagaac tcgaagatat ttacaggccc ttcagaccta agaggagaac aagagctacc
    16441 atagctaaag aaaaaggatt agagccactg gctcaaatga tatgggaaca agaactcaca
    16501 aatcctgtag aagaaatcgc aaaggattat attgatttag aaaaagaggt aaccagtgta
    16561 gaagatgcca tcaatggagc aatggatatt atagcagaat ggatttccga taatgctgat
    16621 tatcggaaat atatacgtaa ggaaacattt tacaggggca tcttagtcgt aaaggccagg
    16681 gatgaaaaag aagagtctgt ttatgaaatg tactatgact atagtgaagc ggtaaaagag
    16741 attccgggac acaggatact ggctattaac agaggcgaaa aggaaaaatt tttaagcgtt
    16801 aaaattgatg ctccagttga caggattata ctatttttag aaaagcaaat tattaaaaca
    16861 gaaggaccag cccatgagtt attaaaactt gttattgagg acagttataa aagactgatt
    16921 gctcctgcta ttgaaagaga aatacgaaat gaattgacgc agaaagctga aacgggtgca
    16981 ttagaagtct ttagaagtaa tctttcccag ttgttgatgc agccgccgat aaaaggcaaa
    17041 gtggttcttg gcttagaccc ggcctacaga acaggatgta aaattgctgt gattgatgaa
    17101 acaggcaagg tattggatat aactgttatc tatccaacgc ctccgcaaaa taatattgaa
    17161 gaatctaagg cgatcattat tgaccttatt aataaatata atgtagatat tgtttctata
    17221 ggtaacggaa cagcttccag agaatcggaa atgtttattg cagatgtcat caaagaactt
    17281 gaaaaaacgg tgcattatat cattgtaaat gaagctggcg catccgtata ttctgcatca
    17341 cctttggcat cagaggaatt ccctgaatat gacgtaggta taagaagtgc aatctcaatt
    17401 ggaagaaggc ttcaggaccc attagcagag ctggttaaaa ttgatcctaa atccattggg
    17461 gttggacaat atcaacacga tatgaatcag aagagattag aagaaagctt gcagggagtt
    17521 gtagaagatt gtgtaaaccg ggttggtgtt gatttaaata cggcgtctgt ttccttattg
    17581 cagtatgtat caggcattac gagtgcagtg gctaaaaata ttgtagaata tagggaagca
    17641 aacgggaaat ttaagaacag aaaagagctt ttaaaagtta aaaaattagg acctaaagta
    17701 ttcgaacaat gtgcaggctt tttaagaatt cctgaatcag ataatatttt tgataataca
    17761 ggagttcatc cggagtccta tgatgtggca caaagattac tggaccgaat tggtcttcat
    17821 ttaaatgata ttaaagaaaa caaagttaaa aacttaagaa aaaacattca agatattgat
    17881 gcattggctc aaagcttaga agtgggtgta ccgaccttag aagatatcat taaagaatta
    17941 gaaaagccgg gcagagaccc cagagaagat atgcccaagc ctattctaag gagtgatgta
    18001 ttaaaattag aagatttaaa agaaaatatg gtcttaactg gaaccgtccg aaatatcacc
    18061 gattttgggg cttttgtaga tattggcgtt catcaggatg gcttggttca tatttcacag
    18121 atgtcagata aatatattaa acaccccctg gaagttgtaa aagtagggga aattgttaag
    18181 gtagccgtat taagtgttga cataaataaa aagagaattg cattgacaat gaaagggatt
    18241 taaggaaggg agaacaagta tggaagttag gtcgtttaat gatgactatc acgaggtcgg
    18301 ccagttagtt ataaactatt tgcatcatga atatgactct gttgaagaaa tgaaggacaa
    18361 ttggaaagga aaaaatctta tattaaaagt ggcagaggac aacggaaaag tgattggatt
    18421 tggtgctctt gatttagtca gaaaaaaagg ggaaccgaat tcagacttat atggaatagt
    18481 tgttgatcct gcttatcgaa ggcaaggcgt aggaactttg cttatgaagt ctttagtgga
    18541 tgatgctaaa ggtttaggta ttcgctacat ttattcaacc ctggacagaa ataatgaaga
    18601 agggataaac tttttgattc attgtggctt tgaatataag agaagcgttt ttatgatgga
    18661 aatgaatgaa cccgtcatta atgaaattga atgccctgaa ggtattgaga ttaaagagat
    18721 gtccgtttta gaaaatgtag aagaattctt agaggtatat gaagaaatat ttgggcatat
    18781 aaatgataaa gaacaactga cagaaaaaat atcaaaagaa aacaattata actttttctt
    18841 aatgtataga gtaagcaaga gtacaggcac aaagaaaatt atcggatttt gtattgtaga
    18901 aaatattgat gaagatggaa ttaaccgccg tattgatatt ttgggtgtaa tcgataagta
    18961 tgccagaaga ggtttgggta aagtgcttta cattacggtt ttaaatcatt attggtctgt
    19021 gcctgaaacc aaaagaatta ccattagttt gaaaagttca tcagaaaatg ttaagaaatt
    19081 ttatttatct tgtggcatgg aagaaaccta tattcaagaa acttaccggt atgaaatttt
    19141 ataaatattt
//
LOCUS       DAOSUD010000042        17705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2002_length_17705_cov_27.907535, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000042 DAOSUD010000000
VERSION     DAOSUD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 17705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17705
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2002_length_17705_cov_27.907535"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..145
                     /locus_tag="PLG49_06695"
     tRNA            69..145
                     /locus_tag="PLG49_06695"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Asp,seq:gtc)
     gene            224..296
                     /locus_tag="PLG49_06700"
     tRNA            224..296
                     /locus_tag="PLG49_06700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Val,seq:tac)
     gene            331..403
                     /locus_tag="PLG49_06705"
     tRNA            331..403
                     /locus_tag="PLG49_06705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:364..366,aa:Thr,seq:tgt)
     gene            412..493
                     /locus_tag="PLG49_06710"
     tRNA            412..493
                     /locus_tag="PLG49_06710"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Tyr,seq:gta)
     gene            514..589
                     /locus_tag="PLG49_06715"
     tRNA            514..589
                     /locus_tag="PLG49_06715"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Phe,seq:gaa)
     gene            625..697
                     /locus_tag="PLG49_06720"
     tRNA            625..697
                     /locus_tag="PLG49_06720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:Lys,seq:ttt)
     gene            808..2199
                     /locus_tag="PLG49_06725"
     CDS             808..2199
                     /locus_tag="PLG49_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ17048.1"
                     /translation="MAEALLYNHLMDYGESGIYPFHMPGHKLGKGLESFPLLKLDLTE
                     ISGMDNLYEAEGVIAKAQELAAATFAADETVFLVNGSTTGVIASVLGVCNPGDELIIA
                     RNSHCSAYHGMILGDIIPRYINPQVINEYGLLGGISTEDVREAVKSYPDAKAVFITSP
                     TYEGFSSDIKKIADLCHSYGKLLIVDEAHGAHFNFHSAFPKSAVTCGADIVIQSLHKT
                     LPALTQSALLHFKGNRLDKNRVKQMIRLIQTSSPSYILMGMMDWLRSHLDERKDEIFN
                     TYIENLINFRDDLKDLKKIKLLEKELNHRYAIEEIDISRLVFYAGRSNKSGLEIDKIL
                     RDHYKIQMELSSEIHFLGISTIVDTKEAFDYLAEVLIDLDKKLMINEEKTDTYNVIYP
                     ISNVKISPREAFYSKKEKIPLEKSEGKISAEFVVLYPPGIPILSPGEEITRIHIEQII
                     KGLKKKQIIDVII"
     gene            2212..2847
                     /gene="tmk"
                     /locus_tag="PLG49_06730"
     CDS             2212..2847
                     /gene="tmk"
                     /locus_tag="PLG49_06730"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOQ17049.1"
                     /translation="MKGFFITMEGPDGSGKSTQISELKEYLNDKGYDVLLTREPGGTD
                     IGEQIRKIILDVNNSHLSYMAETLLYAASRAQHVEEKIKPALEAGKVVICDRFIDSSL
                     VYQGYARKLGIELVESINQFALMGIQPDITFFFDIKPEEAMDRKQNQKALDRLEKENI
                     SFHHLVYEGYKILLDRYPKRIRRIDASQSIDEVKSQILGEIDRFLKEEGLK"
     gene            2844..3173
                     /locus_tag="PLG49_06735"
     CDS             2844..3173
                     /locus_tag="PLG49_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOQ17050.1"
                     /translation="MKLVMAIIHDDDAQKVMDELSRNQFGVTKLASTGGFLKSGNTTL
                     IIGAGEEKVDDVISIIENKCKSRKQVTTANFPLTSVADGYVPYTIEVTVGGATIFVLD
                     VEQFKKI"
     gene            3192..3635
                     /locus_tag="PLG49_06740"
     CDS             3192..3635
                     /locus_tag="PLG49_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOQ17051.1"
                     /translation="MDMKVGPVQTANITEVKTVKEKVTEKDFAFTLLSKIDESELQEK
                     LERMIEEITVQGKKLAEHMDIKDLKKYRAMVADFMNEVVSRSHKFSRENFLDKRGRHR
                     VYGIVKKVNDNLDELAQEIIKKEKDHLKILAKVDEIRGLLLDIIT"
     gene            3749..4744
                     /gene="holB"
                     /locus_tag="PLG49_06745"
     CDS             3749..4744
                     /gene="holB"
                     /locus_tag="PLG49_06745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOQ17052.1"
                     /translation="MYSFEEIVGHKEIIQGLQQGIKNNKISHSYIFDGIEGIGKKTLA
                     YTYAKTLQCLKGAASPCNECASCKSFDSGNHPDVFFIKSEKKSLGVDEIREGIQKEIE
                     IKPYKYKYKIFIVENADKMTIQAQNALLKTIEEPPAYGIIILISTNYKQFLPTIISRC
                     SLIKFNLLKTEEIKHYFMKSNPSQAENIDLYIAFSEGSIGRVKKMMESEYFMDIRKKV
                     LEWIEKAKTGDLIAIYDIEKEMETYKEDIELILYLMYIWYRDILLIKHIGDNPYIINK
                     DKINILLNQSLNLSYNNIDKILETINNARIQISKNANLRLSLELMLLQIKENANG"
     gene            4737..5624
                     /locus_tag="PLG49_06750"
     CDS             4737..5624
                     /locus_tag="PLG49_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOQ17053.1"
                     /translation="MANVIGVRFRRAGKIYYFDPDGLDIKVGDHVIVETIRGIEYGTV
                     VITNREISEEELVAPLKKVIRIATPEDDEKEKENRVKEVEAFDICKQKIKEHGLEMKL
                     IDVEFTFDNNKIMFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDETKMMGSLGI
                     CGRPLCCSSFLCEFQPVSIKMAKDQNLSLNPTKISGVCGRLMCCLKFEEETYREINDR
                     LPDVGQEVMTPDGKGTVLDINVLRELIKVAINKGEDVTEVIVYPANLVTFDPALKKNK
                     TEDILDEMDELDEVEFEDE"
     gene            5617..6372
                     /locus_tag="PLG49_06755"
     CDS             5617..6372
                     /locus_tag="PLG49_06755"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOQ17054.1"
                     /translation="MSNKPALKEGERLDDLNIKNYFLIQNPRLFCFGMDAVLLSSFTE
                     VKEGEEVIDLGTGNGIIPILLAAKTKGKSFLGLEIQAESVDMARRSVLYNDLSHKIRI
                     DQGDIKKVEEYYKKESFDVVTSNPPYMNEGGGLKNPYEAKAIARHELLCKLEDVIAAT
                     AYLLKNKGRFYLIHRPYRLVDIIFLLRKYRLEPKKMQLVQPYEGKEPNMVLIEAIKNA
                     GTLLKVLPPLIIYDNQGQYTKEVKRLYYEDDII"
     gene            6431..7276
                     /gene="rsmI"
                     /locus_tag="PLG49_06760"
     CDS             6431..7276
                     /gene="rsmI"
                     /locus_tag="PLG49_06760"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOQ17055.1"
                     /translation="MAGVLYLCATPIGNLEDISYRAVRVLKEVDLIGAEDTRHTKKLL
                     NHLDIHTPLTSYHEHNKKTKGPFLIEKLKNGLNIAIVTDAGTPGISDPGEDLVRLAYE
                     NNIKVVPVPGPSALITGLIVSGLSTKRFVFEGFLPTDNKERKERLDKLKKEDRTIILY
                     EAPHRLLNTLKNLYEVLGDRNISVIKELTKKYEEVYRCTITEGIHYYTEVEPKGEFVL
                     VIEALSKEALEEIETKKWEDITIEDHYHFYIQQGIDNKEAIKQVAKDRKISKREVYKI
                     IHKIE"
     gene            complement(7314..7580)
                     /locus_tag="PLG49_06765"
     CDS             complement(7314..7580)
                     /locus_tag="PLG49_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOQ17056.1"
                     /translation="MKSTGVVRKVDELGRVVLPIELRRNLDINEKDALEIFVDNDKII
                     LKKYQPADIFTGSMDDLIDYKGKKISKETIIELVRLAGLELKDN"
     gene            8040..8621
                     /locus_tag="PLG49_06770"
     CDS             8040..8621
                     /locus_tag="PLG49_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOQ17057.1"
                     /translation="MLNYIWAFMILLGIIVAAFTGKMGEVTNTAIASSAEGVEVCFRL
                     LGVLAFWMGIMKIAEKSGLINLLTLKIRPILRFLFPDIPDGHDAQKYIATNIIANVLG
                     LGWGATPPGLLAMKALQELNLKKDEASNAMCMFLIVNISSVQLISVNIIAYRAQYGSR
                     NPSEVIGPSILATTISTIVAIIFGKLMERRNKA"
     gene            8618..9151
                     /locus_tag="PLG49_06775"
     CDS             8618..9151
                     /locus_tag="PLG49_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOQ17058.1"
                     /translation="MKWILYISDFMIPLIVSGVIIYGLLKGINIFDVFIEGAKESVKV
                     ILTIMPTLIGLMMAVGIIRASGSLNMFELLLKPISNLITFPVELIPLIVLRTISSSAS
                     LGLVLDLFKQYGPDSFIGRLVSVMMGCIETIFYTMSVYFMSVGIKKTRYTLAGALIAN
                     LAGIIASFYITLFIFGK"
     gene            complement(9189..10844)
                     /locus_tag="PLG49_06780"
     CDS             complement(9189..10844)
                     /locus_tag="PLG49_06780"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOQ17059.1"
                     /translation="MATQQCYPRNQIGANNPVFSQIRTIIETPFYGNNVVKITSLKEA
                     YKLAAASPGTIVTDIPVYNPEAIGLDADAKVLLFNDGAVSGRAASARRIIGEPGVNTN
                     EYALKISEAVYQTRFKKMYHAEVYVGLDKDFMVKAHLLIPEGQENIMYSWMLNFQYIN
                     EYYFNMYQESVKLENEGDIYVFSDPTWSHPDHPMGLTCFDPQHNCAAILGMRYFGEHK
                     KGTLTLAWAIANRNGYASCHGGQKRYNLPSGRKFVAGVFGLSGSGKSTITHARHNDKY
                     DITVLHDDAFIISTTDGSSIALEPAYFDKTQDYPLTSEDNKYLLTLQNNGATLDENGK
                     VVVVTEDIRNGNGRAVKSKLWSTNRIDKFDEPVNAIFWLMKDPTLPPVLKIKGAELAS
                     VMGATLATKRTTAERLAPGVDPNALVVEPYANPFRTYPLADDYNKFKALFQERNVDCY
                     ILNTGSFMDKKVPPSVTLGVLEAIVEGTAEFKPWGNFSDIEIYEVEGFIPDLNDAEYL
                     DQLKKRMEDRIKFVESRETEKGGFDKLPQEALDALKKVISELQ"
     gene            11155..13101
                     /gene="metG"
                     /locus_tag="PLG49_06785"
     CDS             11155..13101
                     /gene="metG"
                     /locus_tag="PLG49_06785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ17060.1"
                     /translation="MSKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARYKRLRGYDV
                     KFLTGTDEHGQKIERIANEKGIHPKEYVDNIVKWIKELWKIMDISYDTFIRTTDDYHV
                     KSVQKIFKKLYDQGDIYKSTYEGWYCTPCETFFTERQLVDGKCPDCGRDVERIKEESY
                     FFKLSKYQDRLIEYIEDNPDFIQPQARQNEMINNFLKPGLEDLCVSRTSFKWGIPVEF
                     DPGHVVYVWIDALSNYVTALGYFSDNDEEYKKYWPADVHLVGKEIVRFHTIIWPAILM
                     ALGEPLPKKVFGHGWLIIDGGKMSKSKGNVVDPKVLVDRYGSDALRYFLLREIAFGQD
                     GNFNNEALIQRINSDLANDFGNLLSRTIAMIQKYFNGQLPEEKAATAFDQDLKNTAKE
                     TIIKMEEAMEKLLFSDSLAEIWNLIRRANKYIDETQPWVLAKDESNKAQLAGVLYNLA
                     EVLRIVSILIQPFMPATTPKVWNQLGISEGEFTTWDSIKEWGKLPDNIKVNKGEILFP
                     RIDLEDELKKLEEALKKAQEEMVVKTEEKNTEQYISIDDFSKVELKVGEVIECEKVEN
                     ADKLLKSKIKIGDEVRQIVSGIAKYYFPEEMIGKKVVVVCNLKPVKLRGILSEGMILA
                     ASDDNGKLVLVSVDKDIESGAKVK"
     gene            13101..13862
                     /locus_tag="PLG49_06790"
     CDS             13101..13862
                     /locus_tag="PLG49_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ17061.1"
                     /translation="MFFESHAHYDDEAFNEDREDLLSSFPNEGIDYVIQTAADIASSK
                     AGLKLAKEYDFIYCSLGVHPHEVKELNEDNFNVLKKLSKEDKVVAIGEIGLDYYYDNS
                     PRDLQRYWFEKQIEWAIEENLPIIVHSREASQETFEIIKKYDFPKKGVIHCYSGSAQM
                     AEEYIKMGFYIGIGGTVTFKNAKKVVEVVEKIPISSILIETDSPYLSPEPYRGRRNDS
                     RNLKYIAEKIAQIKGLDLDTVARITKENGIKLFEI"
     gene            14148..15149
                     /locus_tag="PLG49_06795"
     CDS             14148..15149
                     /locus_tag="PLG49_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOQ17062.1"
                     /translation="MGHKTRTLFFIMVMFLLCSSSATAYQLMLKEVIIIDNDKVYLYK
                     TPKTTVETFLQEQSIVIDSKDEINVTMNDNIVEGMTIKIIRAVPIQLVLDGREEEAYT
                     KAKTVKDFLKEKKIDEENLVSISSSLDEIISPHMELEIKTRKVETITEESVIPYETEL
                     QETPHLPVGEKKLVQEGKDGLKKISYRVEYIGGEEVNREIISEVIVEEAQKEIIKVGA
                     ENVIEGKDGKIYKYKKVLTMSATAYTASYEDTGKAPGHPAFGITYTGTRARVGTVAVD
                     PNVIPLGTKLYIEGYGYAVAEDIGGAIKGNKIDLYYNTLEEARNFGRKQLKVYILVE"
     gene            15194..16057
                     /gene="rsmA"
                     /locus_tag="PLG49_06800"
     CDS             15194..16057
                     /gene="rsmA"
                     /locus_tag="PLG49_06800"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOQ17063.1"
                     /translation="MELIASASKTKEILNKYPFVFRKKYGQNFLIDSHVLNKIIEGAE
                     IGREDFVLEIGPGIGSLTQVLSREAKKVVAVEIDSQLIPILEDVLKECDNLEIIHGDI
                     LKTDIKKIIEEKNNNQPIKVVANLPYYITTPIIMMLLENKLPVSSITVMIQKEVADRL
                     IARPGTKDYGAITVAVNYYAVPYLVANVPRNCFIPRPNVDSAVIKLELRSEAAVEVKD
                     EELFFKIVKAAFSQRRKKLMNNLISQEITDLSKDELALIFSQLAFDENIRGEMLSIED
                     FARLTNALKKE"
     gene            16141..17406
                     /locus_tag="PLG49_06805"
     CDS             16141..17406
                     /locus_tag="PLG49_06805"
                     /inference="COORDINATES: protein motif:HMM:NF040001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HOQ17064.1"
                     /translation="MSNGNYRSFIILRQDCRGYAISSKEPFGYCKIDAYGDKARFQLY
                     VQELKIIPKEKGYRFTLLANLPAGPKQIPLDSFYVDEKGKRNFKIEMDRNNIGGSGLK
                     VEQIEGVAVLAEDSFSNTVAPLIGFKREPFEWNPKKMRTKFEKEEKMKENNKEEKQAI
                     KERKELKETTTDEKERKEEIKKKEMERSDIEKATEKLKEIKGEDIYKEEKQEERKEEN
                     IIEKAVSEENDETKEIRLFESEKERKEDFTEIFEFKNEELEEKKPDKEERKSDQDKEE
                     SAKIETEDQFFTGQTYWNFFDKRSKEEDYINKIIERSIRMNPFEKPGEDMEWYRISYE
                     ELPLIVNFPWRWYSNPFLLVGAKKYNHILLGKNKKLNTYCLGIPDLYSQYSKERAESF
                     GYRTFLCCRNTQPVTGEYGYWIIDLVMNV"
     gene            complement(17531..>17705)
                     /locus_tag="PLG49_06810"
     CDS             complement(17531..>17705)
                     /locus_tag="PLG49_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOQ17065.1"
                     /translation="RSGGYGRGQNLDAVKNNGIDGHVDIHFLNSRTHGTNVVQKIHQD
                     KVKEAAEYIATHY"
BASE COUNT         6360 a         2457 c         3448 g         5440 t
ORIGIN      
        1 tgtttagctg ataggtacta ataggtcgag ggcttgacct aatataacag tgtttagttt
       61 ttatatatgg cccagtggct cagttggtta gagcgccggc ctgtcacgcc ggaggtcgag
      121 ggttcgagtc ccttctgggt cgccatttaa atatatatca ttgaagtaaa tcttgtcatt
      181 tgctagctaa catccaatat cttatattca aaacttctat tttgggcgca tagctcagct
      241 gggagagcac ctgccttaca agcagggggt cataggttcg agccctattg cgcccatttt
      301 tatacaataa aattttaatc aatttaaggt gccgacgtgg ctcaattggc agagcagctg
      361 acttgtaatc agcaggttat cggttcaagt ccgatcgtcg gcttatatta tggagggatt
      421 cccgagtggc caaagggggc agactgtaaa tctgttgtca aagacttcga aggttcgaat
      481 ccttctccct ccattttatt tacatattct tatgcccaga tagctcagtc ggtagagcag
      541 aggactgaaa atcctcgtgt cgctggttcg attccggctc tgggcaccag atttaacaaa
      601 aaatttacat atttcactga gcatgggcta ctagctcagg cggtagagca ctggactttt
      661 aatccaggtg tcccgggttc gagtcccggg tagctcataa taaagcatgt atttctttgg
      721 aagtacatgc ttttcttaat ttggtttaaa tctatcttat tttttgttat actataagaa
      781 caaaataatt ataataggat tgattttatg gcagaagctc ttttatacaa tcatttaatg
      841 gattacggag aaagtggaat atatcctttt catatgcccg ggcataaatt aggcaaggga
      901 ttggaatctt ttcctttatt aaaactggat ttaactgaaa taagtggtat ggataattta
      961 tacgaagctg aaggcgtcat tgctaaagcc caggagttgg cggcagctac ttttgctgcc
     1021 gatgaaactg tttttcttgt caatggttct accacaggcg ttattgcttc agtacttggt
     1081 gtttgtaatc caggagacga attaattata gcaagaaata gtcattgttc tgcgtatcat
     1141 gggatgattt tgggagatat tattccaaga tatattaatc ctcaagtcat aaatgaatat
     1201 ggcttattag gaggcatatc tacggaagat gtccgggagg ctgtaaagtc ttatcctgat
     1261 gcaaaagctg tattcataac aagtccgact tatgaaggct ttagttcaga tattaaaaaa
     1321 attgcagatc tatgccattc ttatggtaag ttattaattg tggatgaagc ccatggtgcc
     1381 cattttaatt tccattctgc ttttccaaaa agtgcggtaa cctgtggggc agatattgtt
     1441 attcaaagcc ttcataaaac ccttccagct ttaacacaaa gtgcgttatt gcattttaaa
     1501 ggcaatcggt tggataaaaa cagggtaaag caaatgatcc ggctgataca aaccagcagc
     1561 ccttcctata ttttgatggg tatgatggat tggctgagaa gccatttaga tgaaagaaaa
     1621 gatgaaattt ttaatactta tattgaaaac ttaataaatt ttagagatga cttgaaggat
     1681 ttaaagaaga ttaagttatt ggaaaaagaa ctaaatcatc gctatgccat tgaagaaatt
     1741 gacatatcca gattagtttt ttatgctgga agaagtaata agtcaggact tgagatagat
     1801 aaaattttaa gagaccatta taaaattcaa atggagctct ccagtgaaat tcattttctt
     1861 gggatttcaa cgattgtgga tacaaaagaa gcatttgatt accttgctga ggtattaatt
     1921 gatcttgata aaaaattaat gattaacgaa gaaaaaaccg atacatataa tgtaatatat
     1981 cctatttcca atgttaaaat ttctccaaga gaagcttttt atagtaaaaa agaaaagatt
     2041 cccctggaaa agtcagaagg taaaatatca gcagaatttg tagttttata tcctcctggt
     2101 attccaatat tatctccggg agaagaaatt acaaggattc acatagaaca aattataaaa
     2161 ggtcttaaga aaaagcagat tattgacgtt atcatataga cggggtggat tatgaaagga
     2221 ttttttatta cgatggaagg tcctgatggt tcgggaaaaa gcactcaaat tagtgagcta
     2281 aaagagtatt taaatgataa aggttatgat gttttgttga ccagggaacc aggaggaacg
     2341 gatattggcg aacaaatccg gaaaattatt ttggacgtga acaacagtca tttgtcatat
     2401 atggctgaaa ccctccttta tgcagcttcc agagcccagc atgtggaaga aaaaataaag
     2461 cctgccctgg aagctggaaa ggttgttatt tgtgacaggt ttattgattc aagtcttgtt
     2521 tatcaaggtt atgcaagaaa acttggcata gaacttgtag aatccattaa tcagtttgcg
     2581 ttaatgggca ttcagccaga tattactttc ttttttgata ttaaacctga agaagctatg
     2641 gataggaaac aaaatcagaa agcattagac cgcctggaga aagaaaacat ttcattccat
     2701 catcttgttt acgaaggtta taaaattctt ttagatcgtt atccaaaaag aataaggcgg
     2761 attgatgcaa gtcaatccat agatgaggtt aagagccaga ttcttggtga aatagaccga
     2821 tttttaaagg aggagggttt aaaatgaagt tagtaatggc aattattcat gatgatgatg
     2881 cgcaaaaagt aatggatgaa ctcagcagaa atcaatttgg ggttacaaag ttagcaagta
     2941 ccggtggatt tttaaagtct ggtaatacga cactgattat tggagcggga gaagaaaaag
     3001 ttgatgatgt catttcaatt atagaaaaca aatgtaaaag cagaaaacaa gtaacaacag
     3061 caaattttcc tctcaccagt gtagccgatg gatatgtacc ttatacgata gaagtaaccg
     3121 ttgggggagc aacaatcttt gtattggatg tagaacagtt taaaaaaatt tagtttaaag
     3181 gagaattttt catggatatg aaagtagggc cagtccaaac agccaatatt acggaagtaa
     3241 aaactgttaa agaaaaagta acagaaaaag atttcgcctt taccctttta agcaaaattg
     3301 acgaatcgga attacaggaa aagcttgaaa gaatgattga ggagattact gttcagggta
     3361 aaaaattggc tgagcatatg gatattaaag acttaaagaa atacagggca atggttgcgg
     3421 attttatgaa tgaggttgtg tccaggtctc ataaattttc aagggaaaat tttttagata
     3481 aaagaggaag acacagagtc tatggtattg ttaaaaaagt caatgataat ttagatgaat
     3541 tggcccagga aattatcaaa aaagaaaaag atcatttgaa aattttagct aaggttgacg
     3601 aaataagagg cttattatta gatatcatta cataaaaaag aattgataat aatagatctt
     3661 atttaatata ctataataat aaaaatacac tgctgcttat acagcggtat ttttttgaat
     3721 gaaaatttca aggaacagga gattatatat gtatagcttt gaagaaattg taggtcacaa
     3781 agagattata caggggctgc aacaaggaat aaaaaataac aaaatatctc atagctatat
     3841 ttttgatgga atagaaggaa tcgggaagaa aaccttggct tatacttatg cgaaaaccct
     3901 ccaatgtcta aaaggagctg ctagtccatg taatgaatgt gcttcttgta aatcttttga
     3961 ttccggcaat catccggatg ttttttttat aaaatcagaa aaaaagagtt taggggtaga
     4021 tgaaatcagg gaagggatac aaaaagaaat agaaataaaa ccttataaat acaaatataa
     4081 aatttttatt gtagaaaatg cggacaagat gacaattcag gctcaaaatg ccttacttaa
     4141 gaccattgaa gaacctcctg cttatggtat tatcattctt atttcaacga attataaaca
     4201 atttcttcct accatcatat ccagatgttc tcttataaag tttaatttat taaaaacaga
     4261 agaaataaag cattatttta tgaaaagcaa tccatcccag gcagagaata ttgacttata
     4321 cattgctttc tcagagggca gcattggcag ggttaaaaaa atgatggaat cagaatattt
     4381 tatggatata agaaaaaaag ttcttgaatg gatagaaaaa gctaaaaccg gggatttaat
     4441 tgctatatat gacattgaaa aagaaatgga aacttataag gaagatatag aacttatact
     4501 ctatttaatg tatatttggt acagggatat tttattaatc aagcatattg gagataatcc
     4561 atatatcatt aataaagata aaataaatat cttgttgaat caaagtctga acctgtctta
     4621 taataatatt gacaaaattt tggagacgat aaataatgca agaatccaaa ttagcaaaaa
     4681 tgctaattta aggcttagtt tagaactaat gttactacaa ataaaggaga atgcgaatgg
     4741 ctaatgtaat cggagtacgt ttcagaagag cgggtaaaat atattatttt gatcctgacg
     4801 gtttagatat aaaagttggt gatcatgtca ttgttgaaac cataagaggt atagaatatg
     4861 gtactgttgt tattaccaac agggaaatca gtgaagaaga attggttgct cccttaaaaa
     4921 aggtcataag aattgcaacc ccggaagatg atgaaaaaga aaaggaaaat agggtaaaag
     4981 aagtagaggc ctttgatatc tgcaaacaaa aaataaaaga acatggtttg gaaatgaagt
     5041 taattgatgt agagttcact tttgataata ataaaatcat gttttatttc actgcagaag
     5101 gcagaattga tttcagagaa ttggttaaag accttgctgc gatttttaag accagaattg
     5161 agttaagaca aataggtgtc agggatgaaa caaagatgat gggaagttta gggatatgcg
     5221 gcagaccttt atgttgttcc agttttttat gtgaatttca gcctgtttcc attaagatgg
     5281 ccaaagatca aaatttatcc cttaacccca caaaaatttc tggtgtttgc ggaagactta
     5341 tgtgttgttt aaaatttgaa gaagaaactt atagagaaat taatgacaga ttacctgatg
     5401 taggacaaga agtaatgacg cctgatggaa agggaacagt acttgatatt aatgttctca
     5461 gggaactgat taaagtggct atcaataaag gtgaagatgt gacagaagtc atcgtttatc
     5521 ctgcaaatct tgtaactttt gatcctgcat taaaaaagaa taaaacagaa gatattttgg
     5581 atgaaatgga tgaattagac gaggtagaat ttgaagatga gtaataagcc agctttaaaa
     5641 gaaggagaac gtttagatga cctgaacata aaaaattact ttcttattca aaatcccaga
     5701 ttattttgtt ttgggatgga tgcagttctt ttatcttctt ttacggaggt aaaagaggga
     5761 gaagaagtca ttgatttagg gacgggtaat ggaattattc caattttact ggcggcaaaa
     5821 acaaaaggaa aaagctttct tgggcttgaa atacaggcgg aaagcgtgga catggcccga
     5881 cggagtgtcc tttacaatga tttatcccat aaaatcagga ttgatcaggg cgatattaaa
     5941 aaggttgaag agtattataa aaaagagtct tttgatgtag ttacctcaaa tcctccttat
     6001 atgaatgaag ggggaggcct aaaaaatcct tatgaggcta aggctatagc caggcatgaa
     6061 ttattatgca aattggaaga tgtgattgca gcaacagctt atttactaaa aaataaagga
     6121 cgtttttatt taattcatcg tccttacagg ctggtcgata ttatcttttt gcttagaaaa
     6181 taccggctgg aacctaaaaa gatgcagttg gtgcaacctt atgaaggcaa agaacccaat
     6241 atggttctta tagaagccat aaaaaatgca ggaactttac ttaaagtatt accacccctg
     6301 attatttatg ataatcaggg acaatataca aaagaagtga agcgtttata ttacgaagat
     6361 gatattattt agaaaaaatt taaatactat tgtaaaagaa tttgagtata ttgataaaag
     6421 agggtttaag atggccggag ttttatattt atgtgcaacc cctataggaa atttagaaga
     6481 tataagttac agagcagtaa gagttttaaa agaagtggat ttaatcgggg cagaagatac
     6541 aaggcatacg aaaaaactac taaatcattt agatattcat acgcccttaa caagttatca
     6601 tgagcataat aaaaagacaa aaggtccttt tcttattgaa aaattaaaaa atggcctgaa
     6661 cattgccatt gtaacagatg caggcactcc tggaatatcg gatcctggtg aagatttagt
     6721 ccgtctggca tatgaaaata atataaaggt agttccagta cctggacctt cagcccttat
     6781 cacaggtctg attgtttcag gactttcaac aaaaagattt gtttttgaag gctttcttcc
     6841 cacggataat aaagaacgaa aagaaagatt agataaactc aaaaaagaag acagaaccat
     6901 tatactctat gaggcgcctc acaggctttt aaataccctg aaaaatcttt atgaagtttt
     6961 aggtgacaga aatatttctg ttataaaaga actaacaaaa aaatatgaag aggtttatcg
     7021 ttgtaccata actgaaggaa ttcattatta tacagaagtg gagccaaaag gtgaatttgt
     7081 tttagtgatt gaagctttat caaaagaagc attagaagaa atagaaacaa aaaaatggga
     7141 agatattact atagaagatc attatcattt ttatattcaa caaggtatag ataataaaga
     7201 agccataaaa caagtagcta aagatagaaa aataagcaaa agagaagttt ataaaatcat
     7261 tcataaaata gagtaaaaaa taaaaaagcc aatcaacaat tggctttttt attttagttg
     7321 tcttttaatt ctaatccagc taatcttaca agttcgataa ttgtttcttt agaaatcttt
     7381 ttacctttat aatcgattaa atcatccata cttccagtga aaatgtcagc aggttgatat
     7441 ttctttaaga tgattttgtc gttgtcaacg aaaatttcta aagcgtcttt ttcattgata
     7501 tcaagatttc ttcttaattc gataggtaaa actacccttc ctaattcatc aacttttctt
     7561 actacacctg tagatttcat tttttatcct ctcctcttta taaattatta ttcgacatga
     7621 aactacaatg ctaggatacc ataattagaa ataaaagtca acagatttaa aaaaaaaaaa
     7681 tgaaatataa agaattatat acatttttag ttataaaaac ttgacaatat atgactttct
     7741 aagaaaaagt tctgcatttt atgagtttat ttttcttttc attataagaa atgttacatt
     7801 ttttatcaat tcaaacaaaa taagacttgg taaatataaa atgatttatt ttcgacaatt
     7861 cgacaaaaat tgagcttatc aatttttata aagcagttca tatttttaaa caagcagcat
     7921 atatatgtga taaaagtacc agataaaaaa gctggtatat cagacaaaag tactattttt
     7981 ctttttcatt tttttactga aacaggaaaa taaaatttga attagaaaca ggtgaagaga
     8041 tgttaaacta catatgggct tttatgattt tattaggcat tattgtagca gcttttacgg
     8101 gaaaaatggg tgaagtcact aatacggcca tagcctcatc agcagaaggg gtagaagttt
     8161 gttttagatt attaggcgtt cttgcttttt ggatgggcat tatgaaaatt gcagaaaaat
     8221 ccggcctgat taatttatta acgctaaaaa tccgtcccat attaagattt ctttttcctg
     8281 atattcctga tggtcatgat gcccaaaaat atatagcgac caacattatt gcaaatgtat
     8341 tgggacttgg ttggggagcc acaccaccag gcttgttagc tatgaaagct ttacaagaat
     8401 taaatcttaa aaaggatgaa gccagtaatg ctatgtgcat gtttttaatt gttaatattt
     8461 cttctgtcca gcttatttct gtcaatatca ttgcttacag ggcccagtat ggttcaagaa
     8521 atccttcaga agtaatagga ccatctattc tggcaacgac catatcaaca atagtagcaa
     8581 ttatatttgg aaagcttatg gagaggagga ataaagcttg aaatggatat tatatatatc
     8641 cgattttatg attccactga ttgtcagtgg ggttattatt tatggtctat taaagggaat
     8701 caatattttt gatgttttta tagaaggagc taaagaaagt gtaaaagtaa ttttaaccat
     8761 tatgcccaca ttaattggtc tgatgatggc tgtagggatt atcagggcat ctggaagttt
     8821 aaatatgttt gaacttttat taaaaccgat aagcaattta atcacttttc cggttgaatt
     8881 aattcctctt attgtactta gaaccatttc ttcttcagcc agtttgggcc tggttctgga
     8941 tttatttaag cagtatggtc cggactcttt tattggcagg ttggtatcgg tgatgatggg
     9001 ctgtatagaa actatatttt ataccatgtc cgtttatttt atgtccgttg gtataaaaaa
     9061 gacaagatat actctggcag gagctctaat agccaatctg gcagggatta tcgcttcttt
     9121 ttatattaca ttatttattt ttggaaaata aaagtcttaa caataaaaaa ggagcatatg
     9181 ctcctttttt attgtaattc gctgataact ttcttaagtg cgtctaatgc ttcctgagga
     9241 agtttatcga atccgccttt ttcagtttct ctggattcaa caaacttaat tctgtcttcc
     9301 attctcttct ttaattgatc taagtattca gcgtcattta agtcaggaat aaatccttca
     9361 acttcatata tttctatgtc agagaagtta ccccaaggtt taaattcagc agttccttca
     9421 acaattgctt ctaatacacc taaagttact gatggaggaa ctttcttatc catgaaagaa
     9481 ccagtattta aaatgtagca atctacgttt ctttcttgga ataaagcttt aaatttattg
     9541 tagtcatctg ctaaggggta agttctaaag gggttcgcat aaggttctac aaccaatgca
     9601 ttaggatcca cacctggtgc taatctttct gcagttgttc tttttgttgc taatgtagca
     9661 cccattacgg aagcaagttc agctccttta attttgagaa caggaggaag ggttgggtct
     9721 ttcattagcc agaagattgc attaacaggc tcatcaaatt tgtcaatacg gttggttgac
     9781 cataatttag atttaaccgc acgaccgtta ccatttctga tgtcttctgt aacaacaacg
     9841 actttaccat tttcatcaag ggttgccccg ttattttgaa gagttaataa gtatttgttg
     9901 tcttcagaag ttaatggata gtcttgagtt ttgtcaaagt aagctggttc taatgcaatt
     9961 gaagaaccgt cagtagtgga aataataaat gcatcatcgt gaagaactgt gatatcatat
    10021 ttgtcattat gacgtgcatg ggtaatggtg gattttccag agccggaaag accaaacaca
    10081 cctgctacga attttcttcc ggatggtaaa ttatatctct tttgtccacc gtggcaggaa
    10141 gcgtaaccat tgcggtttgc aatagcccat gcaagtgtta aggtaccttt cttgtgttct
    10201 ccaaaatatc tcataccaag aatggctgca cagttatgtt gtgggtcaaa acaagtcagt
    10261 cccattggat gatcgggatg agaccatgta ggatcggaga atacatagat atctccttca
    10321 ttttctaact taacagattc ctgatacatg ttgaagtagt attcattgat gtactggaag
    10381 ttaagcatcc atgaatacat aatattttcc tgaccttctg ggatcaaaag atgggctttt
    10441 accataaagt ctttgtcaag tcctacgtaa acttcagcat gatacatttt tttgaagcgg
    10501 gtttgataaa ctgcttcgct gattttaagt gcgtattcat tagtattaac gccaggttca
    10561 ccaataattc ttcttgctga agctgcacga ccacttacgg caccatcgtt aaaaagaagt
    10621 actttagcat cggcatctaa tccaatagct tctggattat aaacaggaat atcggttaca
    10681 atagttcctg gagaagcagc tgctaattta taagcttctt tcaaagatgt tatttttaca
    10741 acattattgc cgtaaaaagg tgtttcgata atagttctga tttgggagaa tacaggatta
    10801 tttgctccta tttgattcct tggataacat tgttgagttg ccatttaaac cctccttaaa
    10861 ttttgtaaat attatattaa aaatttataa tataaaaaaa agacaaacaa acaaacacaa
    10921 taataaaaat cttgccccag gttataatga tacagtaact gcatgaaaaa agtcaataga
    10981 attgataaaa attcttagtt ttaaaacata ataataatat attttaaaaa aaattatatc
    11041 atacttttgt aaatgatgaa ttgtttttgc tttttttctc cattggatat ataatatttt
    11101 ttaatatatc aatgcatttt acatacaaat ttacaaataa ggtggaggta aaagatgagt
    11161 aaaaaaacat tttatattac tactcctatt tattatccca gtgacaagct tcatatcggt
    11221 cattcttata cgacggttgc tgcagatgct atggcaagat ataaaagact aagaggttat
    11281 gatgtaaaat tcttaaccgg gactgatgag cacggacaaa aaatagagcg tattgccaat
    11341 gagaaaggga ttcatcccaa agaatatgta gacaatattg taaaatggat taaagagctt
    11401 tggaagatta tggacatcag ttatgataca tttattcgta caaccgatga ttatcatgtt
    11461 aaatctgtac aaaaaatatt taaaaaacta tatgaccagg gggatattta taagagtact
    11521 tatgagggct ggtattgtac cccttgcgaa acctttttta cagaaagaca attggtggat
    11581 ggaaaatgcc cggattgtgg aagagatgta gaaagaataa aagaagaaag ctatttcttt
    11641 aaattgtcaa aatatcagga cagattaatt gaatacattg aagataatcc tgactttatc
    11701 caacctcagg caagacaaaa tgaaatgatt aataatttct taaagccagg tcttgaagat
    11761 ttatgtgttt ccaggacttc tttcaaatgg ggaataccgg ttgaatttga tccaggtcat
    11821 gtggtttatg tatggattga tgcactttct aattatgtta ctgctttagg ttatttctca
    11881 gacaatgatg aagaatacaa aaaatactgg ccagccgatg ttcatttggt tggaaaagaa
    11941 attgttcgtt tccatacgat tatttggcct gcaatcttaa tggctttagg cgaaccgtta
    12001 cctaaaaaag tttttggaca tggatggctt attattgatg gaggtaaaat gtcaaaatct
    12061 aaaggtaatg ttgttgatcc taaagttctg gttgaccgtt atggatcaga tgctctcagg
    12121 tactttcttt taagagaaat tgcttttgga caggatggca attttaataa tgaagcttta
    12181 attcaaagaa tcaattcgga tttagcaaac gactttggta atcttttatc caggacgatt
    12241 gctatgattc aaaagtattt taatggacag ttgcctgaag aaaaagcagc aacagctttt
    12301 gaccaggacc ttaaaaatac tgcaaaagaa accataataa aaatggaaga agctatggaa
    12361 aaactattgt ttagtgattc cttagcggaa atatggaatt taatcagacg ggctaataaa
    12421 tatattgatg aaacccagcc atgggtttta gctaaagatg aaagtaataa ggcacagctg
    12481 gctggtgttt tatataattt agcagaagtt ttaaggattg tatctatatt aatccagcct
    12541 tttatgcctg caacaacacc caaagtatgg aatcagcttg gtattagtga aggggagttt
    12601 acaacttggg acagtatcaa agagtggggt aagctgcctg ataatataaa ggttaataag
    12661 ggagaaattc ttttccccag aattgattta gaagatgaac ttaaaaaatt agaagaagcc
    12721 ttaaagaaag cacaagaaga aatggttgta aaaacagagg aaaagaacac ggaacaatat
    12781 atttccattg atgattttag taaagtagaa ctaaaagttg gagaagttat tgaatgtgaa
    12841 aaggttgaaa atgcggataa attattaaaa tcaaaaataa aaattggtga tgaagtaaga
    12901 caaattgttt ctggtattgc aaagtattat tttcctgaag aaatgattgg aaaaaaagtt
    12961 gttgtcgtat gtaacttaaa acctgtaaaa ttaagaggta tcctttcaga aggaatgatt
    13021 ctggcagctt ctgatgataa tggaaaattg gttcttgttt ctgtagataa agacattgaa
    13081 agcggagcga aggtgaaata atgttttttg aatctcatgc acattatgat gatgaagcat
    13141 ttaatgaaga cagggaagac ttattgtctt cctttcctaa tgagggaata gattatgtaa
    13201 tacagacagc tgccgatatt gcttcatcaa aagcagggtt aaaactggca aaagaatatg
    13261 attttattta ctgcagctta ggggttcatc ctcatgaagt taaagaatta aatgaagata
    13321 attttaatgt attaaaaaaa ttatctaaag aagataaggt tgttgctata ggagaaatag
    13381 gactggatta ttattatgac aattcaccaa gagatttgca aagatattgg tttgaaaagc
    13441 aaatagaatg ggcaatcgaa gaaaatttgc ctattattgt acacagcaga gaggcaagcc
    13501 aggagacttt tgagatcata aaaaaatatg attttcctaa aaaaggggtt attcattgct
    13561 attcgggcag tgcccagatg gcagaagagt atataaaaat gggtttttat attggaatag
    13621 gaggaactgt aacttttaaa aatgcaaaaa aagtggtaga ggtcgttgaa aaaatcccca
    13681 tatcttctat tttgatagaa accgattccc cttatctgag tcctgaacct tacagaggaa
    13741 gaagaaatga ttccagaaat ctaaaatata tagcagaaaa aatcgcccaa attaagggcc
    13801 ttgatttaga tacggttgcc cgcatcacaa aggaaaatgg tataaaattg tttgaaattt
    13861 gagaaagatt aagtttatat tacaaagatt tatattattt ttaaaaagcc aaaatatgta
    13921 aggtgccaaa aagccttatg tattctggct tttttatatg ttaagaaatt ataaaaaagt
    13981 ttttacaaaa tgtaatattt ttatgaataa aatttaaaag atgcgtatat aatagtaata
    14041 tcaaggcttc cgtgaattgt aaaaaatata catttagtta tttgacaatt cacccgttac
    14101 aaattataat acctaagaat ccaaattgaa aaataggagg taaaaaaatg ggacacaaaa
    14161 ccagaacact gtttttcatc atggtcatgt tcttgttgtg ctccagcagt gcaacagcat
    14221 atcaattaat gttgaaagaa gtaattataa ttgacaatga taaagtttat ttatataaaa
    14281 caccgaaaac tacagttgaa acttttttac aagaacaaag catagttatt gattccaaag
    14341 acgagattaa tgtgaccatg aatgataaca tagtggaagg catgacgatt aaaatcataa
    14401 gagccgttcc aattcaatta gttttggacg gcagggaaga agaagcttat acgaaagcaa
    14461 agacagtaaa agattttctt aaggagaaaa aaattgacga ggagaatctg gtgagtatca
    14521 gtagctctct ggacgaaata attagtcctc atatggaatt agagattaaa acccgtaaag
    14581 tagaaacgat tacagaagaa agtgttattc catatgaaac agagttacag gaaacaccac
    14641 atcttcccgt aggtgagaaa aaacttgtcc aggagggaaa agacgggcta aagaaaatta
    14701 gctatagagt cgaatacata ggaggggaag aagttaacag ggaaataata tctgaagtta
    14761 tcgtagaaga agctcaaaag gaaattatta aagttggagc tgaaaacgtc attgaaggaa
    14821 aagacgggaa aatttataag tacaagaaag ttcttacaat gagtgctact gcctatacgg
    14881 caagctatga agatacggga aaggcgccag gacatccggc tttcggtatc acttatacgg
    14941 gtacaagagc cagggtggga acagtagcgg ttgatcccaa tgttattcct cttggaacaa
    15001 aactctatat tgaaggttat ggatatgctg ttgcagaaga tataggcgga gctattaaag
    15061 gaaacaaaat tgacttgtat tataatactc ttgaagaggc aagaaatttt ggaagaaaac
    15121 aattaaaagt atatatttta gttgaatagg aaaagccgat ataatcggct tttttctaat
    15181 ggaggatagg attatggaat taattgcatc tgccagtaag acaaaagaaa tactcaacaa
    15241 atatcctttt gtttttagaa aaaaatatgg acaaaatttt ttgattgata gccatgtctt
    15301 aaacaaaatc atagaagggg ccgaaattgg ccgtgaagat tttgttttgg agataggtcc
    15361 tgggatagga agcctcactc aagtcttatc aagagaggct aaaaaagtgg tggctgtaga
    15421 aattgattct cagttgatac ctattttaga ggatgtttta aaggaatgtg acaatttaga
    15481 gattattcat ggagatatct taaagacgga cattaaaaaa ataatcgaag aaaaaaataa
    15541 taaccaaccc ataaaagttg ttgcaaatct tccttattat attacgacgc caattattat
    15601 gatgctttta gaaaataagc tgcctgtttc aagtattacg gttatgattc aaaaagaagt
    15661 agcagacagg ttaattgcca gaccagggac aaaagattat ggagcgatta cagttgccgt
    15721 aaattattat gcggttccat atcttgttgc gaatgtccca agaaattgtt ttattccaag
    15781 acctaatgtg gattctgctg taataaagtt agagttgcgt tcagaagctg ccgtagaagt
    15841 caaagatgaa gaactatttt ttaaaattgt aaaggctgct ttttctcaaa gaaggaaaaa
    15901 gctgatgaat aatttaatta gccaggagat aacagatctt tctaaagatg aacttgcttt
    15961 aatatttagt caattggctt ttgatgaaaa tatcaggggt gagatgcttt cgattgagga
    16021 ttttgcacga ttaaccaatg ccttgaaaaa agaataaata gcatattttt aactctccat
    16081 aaatatatta taaagacaag atatgttagg aatctggaca ttaagaatgg agggttagga
    16141 ttgagtaatg ggaattaccg ttcatttatt attttaagac aagactgcag gggctatgct
    16201 atatccagta aggagccatt tggctactgt aaaatagatg cctatgggga taaagcaaga
    16261 ttccagctat atgtacagga attaaaaatt attccaaaag aaaagggtta ccgatttacc
    16321 ttgctggcta acttacctgc gggtcctaag caaattcctt tggatagctt ttatgttgat
    16381 gaaaaaggta agcggaattt taaaattgag atggaccgaa ataatatagg cggaagcggc
    16441 cttaaagtgg agcaaattga aggggtagca gtcttagctg aagattcttt ttctaataca
    16501 gtggctcctt taataggttt taaaagagaa ccttttgagt ggaatcctaa aaaaatgcga
    16561 acaaaatttg aaaaagaaga aaaaatgaaa gaaaataata aagaagaaaa gcaagcaata
    16621 aaagaaagaa aagaattaaa agaaactact acagatgaaa aagaacgaaa agaagaaatc
    16681 aagaaaaaag aaatggaaag atcggatata gaaaaagcaa cagaaaagct caaagagatt
    16741 aaaggagaag atatttataa agaagaaaag caggaagaaa gaaaagaaga aaatataata
    16801 gaaaaagctg tatcagaaga aaacgatgag acaaaagaaa taagattatt tgaaagtgaa
    16861 aaagaaagga aagaagattt tactgaaatt tttgaattta agaatgaaga gctagaagaa
    16921 aaaaagccag ataaagaaga aagaaaatca gatcaagata aagaagagtc agccaaaatt
    16981 gaaacggaag atcaattttt tactggtcaa acttattgga acttttttga taaaagatcc
    17041 aaagaagaag attatatcaa taaaattata gagagaagta ttcggatgaa tccttttgaa
    17101 aagccaggtg aagatatgga atggtataga atatcctatg aagaacttcc ccttattgta
    17161 aatttcccct ggcgctggta ttcaaatccc tttttattgg ttggggctaa aaaatacaat
    17221 catattcttt tgggtaagaa taagaaatta aatacttatt gtttaggaat tccggactta
    17281 tattctcagt attctaaaga aagagcagaa tcttttggct acagaacatt tttgtgttgt
    17341 agaaatactc aaccagttac aggagaatat ggctattgga ttatcgattt agtcatgaat
    17401 gtttagtttt aaaaaaggtc tgttgcattg acagttaaat caattaatgt attgttttac
    17461 tttagtatta aaaaaggagc tgtacgtaaa taaattttcg taacagctcc tttaatttta
    17521 acttttattt ttaataatgt gtagcaatat attcagcagc ttctttcacc ttatcctgat
    17581 ggattttttg taccacattg gtaccgtggg ttctgctgtt taaaaaatgt atgtccacat
    17641 gaccatctat gccattgttt ttaacggcat ctaaattttg tcctctgcca tatcctccac
    17701 tgcgt
//
LOCUS       DAOSUD010000043        17333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2049_length_17333_cov_24.529865, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000043 DAOSUD010000000
VERSION     DAOSUD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 17333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17333
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_17333_cov_24.529865"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1273
                     /locus_tag="PLG49_06815"
     CDS             377..1273
                     /locus_tag="PLG49_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOQ17066.1"
                     /translation="MKKRLKVKNDLIFQKLFGRQDSKDSLVSLINSVLQFDEERKVED
                     VNVIEQQEELPVDISKVENQGKVDVVAKTKDGQSINVQLQIINRFNTDKKVLYNWSRL
                     FCEGLDMGQANKTISINFLDFDFIESDNYHTVFHLTEDTNNHCKFTDLLEIHFIELSK
                     FRKAKPDINRPLERWLLFIEPTSAEVLEMITYRDPGISKAEKLLESLSVDEEVLKLYE
                     LREKALSEEESKFSGAREEGKKEGIIEGLYLVARNMINAGMDIHTIKKVTGLKAEEII
                     ALQTSMQNNNQPQAPQPPFNQF"
     gene            complement(1335..2357)
                     /locus_tag="PLG49_06820"
     CDS             complement(1335..2357)
                     /locus_tag="PLG49_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17067.1"
                     /translation="MSIILGILSLIAVIIASVAVTFLTGFELLHLSVFLIIPLGSLFI
                     GYWGGYGYFKGLVLSNKYIAFKHFFVGFILSLLCIAAIKYTTYYFTCIDPENNTIIYS
                     YNGDHISNYEAVGYGKLDFLNYNKYMIENTPISFVIKSLDFAEISHPMIGWLSVFIDF
                     LGVISGFILAGLIQRKNPYCHDCKLYKKKKKLFIISKTEKDNFFNQLNDLTSQGKWET
                     AYSMLSKYAFDKNVKNQEHLLCKLSYCKNCNISELNFELYELSYLKRIKKNPYYEHPI
                     TIDYKLTEKLLNKEKAALDTDKNTEFDEKFIDKVNESIKVSEEAKIHEKVKVEEKLET
                     EEEIKL"
     gene            complement(2470..2733)
                     /locus_tag="PLG49_06825"
     CDS             complement(2470..2733)
                     /locus_tag="PLG49_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17068.1"
                     /translation="MKVVMEHIDVIACFDQTKGLIPIKFRIKNKDNSESIIKIDKICY
                     QKQEKIAGNPMIIFICQSLINNVLKKYEIKYEINTCKWYLYKI"
     gene            complement(2747..4006)
                     /locus_tag="PLG49_06830"
     CDS             complement(2747..4006)
                     /locus_tag="PLG49_06830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ17069.1"
                     /translation="MSKMPIIMHIDVNSAFLSWEACYRLQHGDTLDLRTIPSIVGGNP
                     ETRRGIVLAKSIPAKKYNIKTGESIYSALQKCPDLTIVAPRFDLYLQCSDSLMKLLKE
                     YSPIVQRYSIDECFLDYSNMESHFGDPLSAACQIKDRIKNELGFTVNIGISHNKLLAK
                     MASDFKKPDAVHTLYPHEIKEKMWPLPVENLFMVGRATKTKLHKLGIFTIKDLATADP
                     KFLEKHLKSHGLLIWNFANGIENSPVISENPKPIKGIGNSTTIPFDVTDRKTAHLILL
                     SLVETVSMRLRNENARASLISVSIRTSDFKNYSHQKKLANPTDCTNHIFKYVCCIFDE
                     VWTKEPIRKLGVRVSELSCNQFSQISLFDSVDTEKQKSVDKAIDELRLRYGSHCIFRA
                     GFLQSNLNPVTGGITIEEDLPMLSSKL"
     gene            complement(4200..4853)
                     /locus_tag="PLG49_06835"
     CDS             complement(4200..4853)
                     /locus_tag="PLG49_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOQ17070.1"
                     /translation="MIKTTIVGFGDSLTYGYGVPRNVRFIERLEKFMPLYFPNISWNI
                     CNAATSGDTTREGLKKVEKKVLSCNPNIVLILFGSNDSAMNDKQFRSLEEFEDNLRKI
                     ISKIKHHNNRTGLNKCIPIPVLITPPPVREDLWAPVRSNNRLKQYGYIVKSLAKEYHC
                     PLIDFFEHAINYGNYEELLGDDGLHLSEKGYDLLYDLTFSELTKLINYEGVLKDWDE"
     gene            complement(4935..6209)
                     /gene="aroA"
                     /locus_tag="PLG49_06840"
     CDS             complement(4935..6209)
                     /gene="aroA"
                     /locus_tag="PLG49_06840"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOQ17071.1"
                     /translation="MIIKPIETIKGTLSVPGDKSISHRAIMLGAISEGITEISGFLMG
                     DDCLSTVDCFRKLGIKIEISENKVFVHGKGLMGLSAPSTILDVGNSGTTIRLLTGILA
                     GQNFSSSITGDASIQKRPMLRVVNPLRKMGAEIEGIEGGNYCPLHIKGKNLNGIHYKL
                     PVASAQVKSAILLASLYSKGKTIIIEPKSSRDHTEIMINYFGGNILKNKNEIISSPVK
                     KLIGQQVEVPGDISSAAYFIAAALILPNSELLIQNVGINPTRSGIITVFKQMGGDIQL
                     INERIQSGEAVADIFVRSSKLKGIEIKGDIIPKLIDEIPVIAATACYADGVTIIKDAA
                     ELKVKESNRIKTMSSELRKMGAQITETDDGMIIQGTSQLMGTTVESYNDHRVAMSLAI
                     AALRANKETKIQNSECVNISFPNFFSLLSMFE"
     gene            complement(6222..7319)
                     /locus_tag="PLG49_06845"
     CDS             complement(6222..7319)
                     /locus_tag="PLG49_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOQ17072.1"
                     /translation="MNKIGIVGLGLIGGSLAKALIEKSSVRHIVAMDLNENALHQAFN
                     DGVINSYTTTIDEKFSDCDIIFLCTPVNLISDYVNQLLPYVNENCILTDVGSTKQAIM
                     TDLKNLKSEKDFCFIGGHPMTGSEKAGYMASRGHLFENAYYILTPEDSTSQNQLEKLK
                     KLIYSIGAIPLVISPEYHDLITAAISHVPHIIASSLVNMVKNLDNEDKYMNLLAAGGF
                     KDITRIASSSPEIWHNICMTNQNNIRFVLDYFIQILKEFSSAIEEQKDEFIWNFFDSA
                     KQYRKSFSDRSPGPLLKNYEIIVDVVDQPGIIANIAVLLSKHNINIKNIGIINNREHE
                     NGALQIIFDKEEDQKKSIKLLKDMNYNIYEK"
     gene            complement(7312..8337)
                     /gene="aroF"
                     /locus_tag="PLG49_06850"
     CDS             complement(7312..8337)
                     /gene="aroF"
                     /locus_tag="PLG49_06850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOQ17073.1"
                     /translation="MIVVMRPDATEENIQTVIKTIEYCGLKVHLSQGSEVTIIGVIGD
                     KSKLSKSNLELLEGVDKLVPVTESYKLSNKKFFAGPSIIKVGNCEIGGKEIVIMAGPC
                     AVESREQLLASAHAVKKAGAQILRGGAYKPRTSPYSFQGLEEEGLKYMAEAREETGLA
                     IVCEVTSTQAVETAAKYVDMMQIGARNMQNFQLLKEVGKTKIPVLLKRGLCATIDEWL
                     NAAEYIMSEGNPNVVLCERGIRTFETSTRNTLDISAVPVIKAKSHLPIIVDPSHATGI
                     RDFVEPLAKASIAAGADGLMIEVHPDPSKALSDGPQSLNPEAFMKLTKNLKPLIELMG
                     RSFNNHE"
     gene            8645..10720
                     /gene="fusA"
                     /locus_tag="PLG49_06855"
     CDS             8645..10720
                     /gene="fusA"
                     /locus_tag="PLG49_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ17074.1"
                     /translation="MKNYSMNQIRNVVLLGHGGCGKTTISEAMLYVSGQVKRQGKVDD
                     GNTVSDYDPEEVKRKVSINTSIIPIEWKDHKINILDTPGYFDFVGEVKQAVRAADSAI
                     IVVSAKSGVEVGTEKAWDYAEEAGLPKMIFINGMDDEHANMNQILEDLREKFGKSIAP
                     FQVPFKENGRFAGFVNVVKMEGRRFVNGTVEPCEVPAGMDDEIAPVRNMILEAVAETS
                     EELMEKYFNEEEFTPEEIDKALHSGVLDGSIVPVLCGSAIDTLGVRVLLNSILSYMPS
                     PAEAKSSVEGKNPKTGETETRKVDENEPTSVFVFKTIVDPYIGRLSIFKVCSGVLKAD
                     SVLLNGKKDVEEKLSHIYVLKGKEQIEVSQLCAGDIGAVAKLQETMTGDTLSDLKKPI
                     VYPEIEFPESLLMMAVFPKGKGDEDKMSAGLNRLMEEDPTFRVSLNKETLEQIMYGIG
                     DQHLDVIVSKLKAKYKVEVELKAPTIPYRETIKAKVKVQGKHKKQTGGHGQYGDVWME
                     FEPSGDLNVPYVFEEKIFGGAVPKQYFPAVEKGLQESVKNGVLAGYPVVGLKATLVDG
                     SYHPVDSSEMAFKMATSIAFKEGLKKAKPVILEPIVRVTVTVPDEYMGDVIGDLNKRR
                     GKILGMNPKGKYQVVDAEVPLAEMYKYATDLRSMTQARGSFTMEFDRYEEAPMDVQEK
                     VIEARKKEA"
     misc_binding    10773..11014
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11089..13509
                     /gene="leuS"
                     /locus_tag="PLG49_06860"
     CDS             11089..13509
                     /gene="leuS"
                     /locus_tag="PLG49_06860"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOQ17075.1"
                     /translation="MKLSSIKYDHKKIEKKWREIWEKNPINQPGPNKKKYYCLDMFPY
                     PSGNGLHVGHWRGYVLSDVWSRYKVLQNYHVLHPMGWDAFGLPAENDAIKKGIHPKIG
                     TERNVANFKRQLHDISAIYDWDREINTTDPNYYKWTQWIFVKMFEKGLAYEKEMPINW
                     CPSCKTGLANEEVVGGRCERCNSVVTKKNLRQWMLKITAYAERLLEDLKDLDWPEKVK
                     KMQTDWIGKSYGAEIDFQVKDSNKKIKVFTTRPDTLYGATFMVLAPEHEMVAEITTEE
                     QKEKVEEYVQLALTKSSVDRMADKEKTGVFTGAYAINPLNGAQIPIWISDYVLADYGT
                     GAIMCVPAHDERDFLFAKKFDLPIIQVIKKEGEEEQELTEAYTEEGIMINSENFNGIP
                     SSEAKERIADYLEEQGIGKKTVNYKLRDWVFSRQRYWGEPIPIVHCEKCGAVAVPEEE
                     LPVTLPEVESYEPTGTGESPLAAIKEWVNTTCPKCGGPAKRETNTMPQWAGSSWYFLR
                     YADPHNDKELISKEAMKKWLPVDMYVGGIEHAVLHLLYARFYTKFLYDIGVVDFQEPF
                     IRLFNQGMITKDGAKMSKSKGNVVSPDELVEKYGADSLRMYELFVGPPELDSEWDDRG
                     IDGVYRFINKVWKLITENKDANVEATQEMERVRHKLIYEITTRMNDFHFNTVVSGFME
                     YTNKLTEIAKASNGLDKETIETLIILLAPFIPHAAEELWELTGHHESVFKQQWPSYDE
                     EKMKEDTIEIVIQINGKVKDKIEIRIDEAKESVLEKAKEAVKNKMDGKSIVKEIYVPG
                     KIVNLVVK"
     gene            13703..14134
                     /locus_tag="PLG49_06865"
     CDS             13703..14134
                     /locus_tag="PLG49_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ17076.1"
                     /translation="MKVRDIMTQNVISVDEEESVLRASQVMRDANVGSVPVVRGDKLV
                     GIITDRDIVVRNVADGEDVDNVTCKNIMTTDLVTCSPDMDVDEVAALMSKHQVRRIPV
                     VENGKVVGMVSLGDLATQRTTQDEAEEALKNISSPSMPEYQ"
     gene            14197..15078
                     /locus_tag="PLG49_06870"
     CDS             14197..15078
                     /locus_tag="PLG49_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17077.1"
                     /translation="MKNNKIIICVLILFSLVTTPLLYYISDNPGLIQGKLIHNSMLNK
                     PGGKSKVIYDLNVPLDNHLISRMKIYEAKTKEIEEQEIKHYVKVLKLNFSDIKEDVSA
                     YRIQNDHYQLIVDKNTGLIQYKNLEINIEDNNKKINPEEAIEIFKAFAEKLNLPLNYR
                     KCLVEDIPDKKLYQLRFINSIEGILNYGYCTKAELSHKGEILNFNYYQLVYKPRQIVS
                     IKTKKEAYEDLLKIPFEEEAHIDIQKIELVYFTQDIEKKNREKFEDEFILIPCYRFFG
                     EISKNKSFEYFIPALKE"
     gene            15170..15382
                     /locus_tag="PLG49_06875"
     CDS             15170..15382
                     /locus_tag="PLG49_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17078.1"
                     /translation="MSKKETEFDRYERMKKIQREMEIQKRKEMDDFRAGKNKKKKKMG
                     FSENNTRRKDWTKYYEADMDDYDEVY"
     gene            complement(15438..15698)
                     /locus_tag="PLG49_06880"
     CDS             complement(15438..15698)
                     /locus_tag="PLG49_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17079.1"
                     /translation="MKRDILKDLDPDMIKEATDVMNSLKGKSDQEILEALIKLAAQRK
                     QNGKKLTREQNEAILEAMKESLPSEQRKKFEAIIQMMKILNT"
     gene            complement(15721..16362)
                     /locus_tag="PLG49_06885"
     CDS             complement(15721..16362)
                     /locus_tag="PLG49_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17080.1"
                     /translation="MRDENQAPPETNQEQENNGYDISKMLEMAKLFSSLMNHKASEEN
                     KAVSVPVTNPSPVISPNATILFDETIHTPELKVVKAAIPYFEPYHQKILGVFVKAMEF
                     KRVIDMYYKNPENPLINMNLKSNPYWKTEMLNSFKPHCSHENQYLIDIMVKVMDIGEL
                     MKKIQSLKIAEEVKKIPETTEKKNTKQKLIESLSPLLDDNQKQMLNLFSSLIE"
     gene            16586..17059
                     /locus_tag="PLG49_06890"
     CDS             16586..17059
                     /locus_tag="PLG49_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17081.1"
                     /translation="MDYLLKDIESLLGTHIDFKEDIRQIRESIINLFKEKYFNKIQSL
                     IEENRIQAQENPTKEMTLLKALKPFMDSKNHQMLDEIEKFMNTIRVLENVNQKLKSLH
                     RQSNDDDSKEEKKEKIIVDQDGVYEIDEACINNIQRESKGKEILLLFLIILLLKR"
     gene            complement(17098..17304)
                     /locus_tag="PLG49_06895"
     CDS             complement(17098..17304)
                     /locus_tag="PLG49_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17082.1"
                     /translation="MADSKSYCGGTNTLLFLFFFLLLFNNGGGLFGYESKGQGGFGDF
                     ADNSLFFFLIIFLILFGGYGGGVC"
BASE COUNT         5930 a         2600 c         3180 g         5623 t
ORIGIN      
        1 aaatgtttta aggcaattat atctaaatta tatcaaatat ttaatattat tttaattata
       61 attatcgaca aaatattaaa aagttaactg atccatttat tttataagta gcacgaatat
      121 agataatagt aactataatg cattcagcaa aagttttttt taaaatattt tatatcttga
      181 aaaaataata tccttatact ataattgtat atgtaaatgt tatttattct gatttttgtt
      241 aatagattta cagttaataa attttttact atataatttc gaaattggca attttttata
      301 attagtctgc tttactaaat gattgtcgtt tattttttta aaataaaatc aaattatata
      361 attaaaggtg atgaatatga agaaacgctt aaaagtaaaa aatgatttga tttttcaaaa
      421 gctgttcgga agacaagata gtaaagacag tcttgtaagt ttaattaatt cagtgttaca
      481 gtttgatgaa gaaagaaaag tggaggatgt aaacgttatt gagcaacaag aagagcttcc
      541 tgtggatatt tctaaagtag aaaatcaggg taaggttgac gtcgtggcaa agacaaaaga
      601 tggacaaagc ataaatgttc aacttcaaat tattaatcgt tttaatacgg acaagaaagt
      661 actttataat tggtcaagat tattttgtga aggtttggat atgggtcaag ccaataagac
      721 aatatcaata aatttcttgg actttgattt tattgaatca gataattatc acactgtatt
      781 tcatctaaca gaagatacca ataaccattg taaatttacg gacttattag aaattcattt
      841 tattgaatta tctaagttta gaaaagctaa accagacata aaccgtcctt tagaaagatg
      901 gttactattt atagagccta cttctgcaga agtattagaa atgattacat atagggatcc
      961 tggtatttca aaagctgaaa aattattaga aagcttaagt gtggatgaag aagttctaaa
     1021 attatatgaa ttaagagaaa aggctctcag tgaagaagaa agcaaattct caggagccag
     1081 ggaagaaggt aaaaaagaag gaatcattga aggactttat ttagtagcac gtaatatgat
     1141 taatgctgga atggatatcc ataccattaa gaaagtaact ggcttaaaag cagaagaaat
     1201 tattgcattg caaaccagta tgcagaataa taatcaacca caggcaccac aaccaccttt
     1261 taatcagttt taattaagct taaaaaaatt gcggcatata ataaatatgc cgctttttat
     1321 gtaagtattt tttattaaag tttgatttct tcttcagttt caagtttttc ttcaacttta
     1381 actttttcat gaattttagc ttcttcacta actttaatgc tttcattaac tttgtctata
     1441 aatttttcat caaattccgt atttttgtct gtatctaaag cagctttttc tttatttaaa
     1501 agtttttctg ttaacttata atcaatagtt attggatgtt cataataggg atttttcttt
     1561 attcttttca ggtagcttag ttcgtaaagt tcaaaattta attctgaaat attacagttt
     1621 ttacaatagg ataatttgca aagcaggtgt tcttgatttt ttacattttt atcgaaagca
     1681 tatttactta acatagaata ggcagtttcc cacttacctt gtgaagttaa gtcattaagc
     1741 tgattaaaga aattgtcttt ttctgtttta gatataatga ataatttttt tttcttttta
     1801 tagagcttac agtcatgaca atagggattt tttctttgaa tgaggccagc aaggataaag
     1861 cctgatataa caccaagaaa atcaataaat acagacaacc agccgatcat tggatgggat
     1921 atttcggcaa aatcaagact ttttataaca aatgaaatag gcgtattttc aatcatatat
     1981 ttattatagt ttaaaaagtc taattttccg tatcccaccg cttcataatt gctgatatga
     2041 tctccgttgt aggaataaat aatggtattg ttttcaggat ctatgcaagt aaagtagtaa
     2101 gtggtatatt taatcgctgc gatgcataaa agagataaaa taaagccaac aaaaaaatgc
     2161 ttaaaggcaa tatatttgtt tgataatacc aaacctttaa aataaccata gccgccccaa
     2221 tagccaatga agagacttcc taaaggtatg ataagaaata cagacaaatg taatagctca
     2281 aaacctgtta gaaaagttac agcaactgaa gcaataatga cagcaattaa tgacagaatc
     2341 cctaatatga tactcatttt ttcctcctaa gttatgagat attttgtaaa aatttattag
     2401 tataatttta tacaactgta agaaaattat caagattaac tttaaagttg aatattagga
     2461 ggatattttt taaattttat ataaatacca tttacaagta ttgatttcat atttgatttc
     2521 atactttttt aagacattat taataagact ctgacagata aaaataatca taggattacc
     2581 tgctattttt tcttgcttct gataacagat tttatcgatt tttataatgc tttctgagtt
     2641 atctttgttt tttatgcgaa attttatagg tataagtcct tttgtctgat caaaacaggc
     2701 aatgacatca atatgttcca tgacaacttt cataagcaac acctacttat aacttgctgg
     2761 ataacatagg taaatcttct tcaatggtta ttccccctgt cacaggattt aaattggatt
     2821 gtaagaaacc tgctctaaat atacaatggc ttccatatcg taatcttaat tcatcgatcg
     2881 ctttatcgac agatttttgt ttttctgtat cgacagaatc aaatagagaa atttgagaaa
     2941 attgattaca tgataattca gatactctta ccccaagttt acgaatcggt tctttagtcc
     3001 aaacttcatc aaaaatacag cagacatatt taaaaatatg gtttgtacag tcagtaggat
     3061 tagctaattt tttttgatgg gaataatttt taaaatcact tgttcttatg gatacactaa
     3121 tcaagctggc acgggcattt tcgtttctaa gccgcatcga gactgtttca actaaagaaa
     3181 gcaggattaa atgggctgtt tttctatccg tgacatcaaa aggaattgtt gtagaatttc
     3241 cgataccttt aatcggttta gggttttctg aaattactgg agaattttcg atgccatttg
     3301 caaagttcca gattaaaagt ccatggcttt taagatgttt ttctaaaaat ttagggtcgg
     3361 ctgttgctaa atccttaatt gtaaagattc ctaatttatg aagttttgtt tttgtagctc
     3421 tgccaaccat aaataaattt tctacaggca gaggccacat tttttcttta atttcgtgag
     3481 gatataaagt gtgtacggca tctggttttt taaaatcaga ggccatttta gcaagcagtt
     3541 tgttatgaga aatcccaatg ttgactgtaa atcctaattc atttttaatg cggtctttga
     3601 tttgacaggc agctgataaa ggatccccaa aatggctttc catattgctg taatcaagaa
     3661 aacattcatc aatgctataa cgttgaacaa taggggagta ttctttgagt aatttcatta
     3721 aactgtcgct acactgcaga tataagtcaa atcttggagc gacaatagta aggtcaggac
     3781 atttttgtaa agcagaatat atagattctc ctgtttttat gttatatttt ttagcaggaa
     3841 tcgatttagc cagtacaatg ccccgccttg tttcaggatt acctcctact atggaaggaa
     3901 tggtgcgcag gtccagtgta tcgccgtgct gtaagcgata acatgcttcc cagcttaaaa
     3961 aagcggagtt tacatcaata tgcataataa taggcatttt agacataaaa ttacctcctg
     4021 tcaccatgac taatatcatt atatccgaac atacgttctg tgtaaacaca cttttttata
     4081 aaatttattt aagataagga ctttccttaa taaaaaatac ggactgtgcc ctaacggtta
     4141 tttaagtgtt agatatagtt caataaaaaa gaactgtaca caattttgtg tacagttctt
     4201 tattcatccc aatcttttaa aacaccttca taattaatga gtttagttaa ctctgaaaag
     4261 gtcaggtcat ataaaagatc gtagcctttt tcacttaaat gtaagccatc atcacctaat
     4321 agttcttcat aatttccata attgattgca tgctcaaaga aatctatcaa aggacagtga
     4381 tattcttttg caaggctttt tacaatataa ccgtattgtt ttaaacggtt attgcttcta
     4441 acgggtgccc ataaatcttc ccggactgga gggggggtta taaggacagg aataggaata
     4501 catttgttta gtcctgtacg attattatga tgtttaattt tactgatgat ttttcttaaa
     4561 ttgtcttcaa attcttctaa agatctgaat tgcttatcat tcatagcaga atcattggag
     4621 ccaaataaaa ttaatacgat attaggattg catgacaaga cctttttttc gactttctta
     4681 agtccttctc ttgtcgtatc cccgctggtt gcagcattac aaatattcca gcttatattg
     4741 ggaaaataca aaggcataaa cttttctagt ctttctataa atcttacatt cctaggaaca
     4801 ccatatccat aagtcaaact gtcaccaaaa ccaacaatgg ttgttttaat cattttccac
     4861 cctccaaaaa aagagcatga cattcacata gatacattat aattcattat ctgcaaatat
     4921 acaacaagct tcaatcattc gaacatgctt aatagtgaaa agaaattagg gaaagagata
     4981 ttgacacatt cactgttttg tatctttgtt tctttgttgg ctctaagggc agcaatggcc
     5041 agggacatag ccactcggtg gtcgttatag ctttcaactg tagtccccat aagttgactg
     5101 gttccctgaa taatcattcc gtcatcggtt tcagtaattt gggctcccat ttttcgaagc
     5161 tcagagctca tggttttaat acgatttgat tcttttactt taagttctgc agcatcttta
     5221 atgatggtaa ctccgtcagc ataacaggct gtggcagcaa taaccgggat ttcatcaatt
     5281 aatttaggga taatatcccc tttaatttcg atacctttaa gtttagaaga acgaacgaat
     5341 atatcggcta cagcttcacc cgattgaatt ctctcattga ttaattggat atcaccgccc
     5401 atttgtttaa atacagtaat aatgccactt cgggtaggat ttatgcctac attttgaatt
     5461 aataattcag aattgggtaa aattaaagca gcggcaataa aataagcagc ggaagagata
     5521 tcaccaggta cttctacctg ttggccaatt aattttttaa ctggggaaga gataatttca
     5581 tttttatttt taaggatatt tccaccaaaa taattaatca taatttcagt atgatctctt
     5641 gaactttttg gctcaatgat gatagttttt cctttagaat aaagagaagc cagaaggatt
     5701 gctgatttta cctgggcact ggcaaccgga agtttatagt gaattccatt taaattttta
     5761 cccttaatat ggagaggaca ataatttcca ccctcaatac cttctatttc agcacccatt
     5821 tttcgaaggg gattgaccac tctgagcatg ggtctctttt gaatggatgc atctccggtt
     5881 attgaagatg aaaaattctg accagcaagg attccggtta aaagtcttat cgttgttcca
     5941 ctgtttccta cgtctaatat cgttgaagga gcagacagcc ccataagtcc ttttccatgg
     6001 acaaaaactt tattttctga tatttcgatt ttaataccca gctttctaaa gcaatccaca
     6061 gtagacaggc aatcgtcacc cattaaaaaa ccagagattt ctgttattcc ttctgaaata
     6121 gcaccaagca ttatggctct gtgagaaata gacttatcac caggaacgct gagggtccct
     6181 ttgatagttt ctattggttt gataatcata tttttacccc tttatttttc gtaaatatta
     6241 taattcatat cttttaggag tttaatactt ttcttttgat cttcttcttt atcaaagata
     6301 atctggaggg caccattttc gtgttccctg ttattaataa tgccaatgtt tttaatattg
     6361 atattgtgtt tgcttaaaag aacggcaata ttagcaatga tacccggttg gtcaacaacg
     6421 tcaacaatga tttcatagtt tttaagcaga ggtcccggac ttctgtcgga aaagcttttt
     6481 ctatattgct ttgcactatc aaagaagttc cagatgaatt catctttttg ctcttctatg
     6541 gcagaagaaa attcttttaa gatttgaata aaataatcca gaacaaagcg tatgttattc
     6601 tgatttgtca tacatatatt atgccagatt tctggtgaag aggaagcaat acgggtaata
     6661 tccttaaagc ctcctgcagc cagaaggttc atgtatttat cttcattgtc caaatttttg
     6721 accatattga ctaaggaaga agcaatgata tgaggtacat gactaatggc agccgtaatt
     6781 aaatcgtgat attcggggga aataacaaga ggtatagccc ctatggagta aatgagtttt
     6841 tttaattttt ctaattgatt ttgagaggta gaatcttctg gtgtaagtat ataataagcg
     6901 ttttcgaaca ggtgtcccct ggaagccatg taacccgcct tttcagaacc agtcattgga
     6961 tgaccaccaa taaaacaaaa gtctttttca ctctttaaat tttttaaatc cgtcatgata
     7021 gcctgtttcg tacttcctac atctgtaagg atgcaatttt cattaacgta tggaagtaat
     7081 tggttgacat aatcgctaat aaggtttacg ggagtacata aaaagataat gtcacaatct
     7141 gaaaactttt catcaattgt tgttgtataa gaattgataa caccatcatt aaaagcttga
     7201 tggagagcat tttcatttaa atccatggca acgatatgac ggacagaaga tttttctata
     7261 agtgctttgg ctaaagagcc accaataaga ccaagcccca caatacctat tttattcatg
     7321 attgttgaaa cttcttccca tcaattctat taaaggtttt aagtttttag tcaacttcat
     7381 gaaagcttca ggattaagag attgtggccc gtcggataaa gcttttgaag gatcaggatg
     7441 gacctcaatc atgagaccgt cagcacctgc agcgatagaa gccttagcaa gtggctccac
     7501 gaaatctctt attcctgttg catggcttgg gtccacaata atgggaagat gactctttgc
     7561 cttaataacg ggcacagcac taatatctaa agtatttctt gtagaggttt caaaagttct
     7621 tattcctctt tcgcaaagaa caacattggg gtttccttca ctcatgatat attctgccgc
     7681 atttaaccat tcgtcaatgg tagcacacaa acctcttttt aataaaacag gaatttttgt
     7741 cttgccgact tctttaagca gttggaaatt ttgcatgttt cttgcaccaa tttgcatcat
     7801 gtctacgtat tttgctgctg tttcgacagc ttgtgtactg gttacttcac aaacaatagc
     7861 aagacctgtt tcttctcttg cttctgccat atatttaagg ccttcttctt caagaccctg
     7921 gaaagagtaa ggggaagtac ggggtttgta agcaccaccc ctgaggattt gtgcacctgc
     7981 tttttttacg gcgtgagcag aagctaaaag ttgttccctg ctttctacag cacaaggtcc
     8041 agccatgatg acgatttctt taccgccaat ttcgcaattt ccgactttaa taatggaagg
     8101 accggcaaag aattttttat tggacagttt ataagactca gtgacaggaa ctaatttgtc
     8161 tacgccttct aaaagttcca gattagattt gcttaattta gatttatctc cgataacgcc
     8221 tatgatggtt acttcagagc cctgagacag atgtaccttt aacccgcaat attctatggt
     8281 tttaatgaca gtttgaatat tttcttcagt agcatccgga cgcattacta caatcataat
     8341 aaagcctcct tatataatca ttctaagaat gttcttaaca ttttacaacg ataaatcata
     8401 tatgtcaatt attgaataga tttatcggct gaaagaaaca aattagtata ttttttcgga
     8461 tattttttag agagggtgag aatcttgctg tgtaaataat ccaaattgct atagtaaaaa
     8521 ataaaatatt tgtgtaaaat tattaataat agagaaggaa aagaatattt tatgtggaat
     8581 atattaaata tgtgaaaatg agtccgaaag gactttacaa aaatataaac gggaggaaac
     8641 tgtaatgaaa aactatagca tgaaccaaat acgcaacgtc gtacttttag gtcacggcgg
     8701 atgcggaaag actaccattt cagaggctat gttatatgtt tctggtcagg ttaaaagaca
     8761 ggggaaagtc gacgatggta ataccgtaag cgattatgat cctgaagaag taaaaagaaa
     8821 ggtatctatt aatacttcta ttatccctat agaatggaaa gatcataaga ttaatatttt
     8881 agatacgcca ggatattttg attttgtagg agaagtcaaa caggctgtaa gagctgcaga
     8941 ttcagccatc attgttgttt cagctaaatc aggggttgaa gtgggaactg aaaaagcttg
     9001 ggattatgca gaagaagctg gtcttccaaa aatgattttt attaatggta tggatgatga
     9061 acatgccaat atgaatcaaa tattagaaga cttgagagaa aagtttggaa aatccattgc
     9121 gcccttccag gttcccttta aagaaaatgg tcgctttgct ggttttgtga atgttgtaaa
     9181 aatggaagga agaagatttg ttaatggaac cgttgaacct tgtgaagttc ctgcaggaat
     9241 ggatgatgaa attgctccgg taaggaatat gattttagaa gctgttgctg aaaccagtga
     9301 agaacttatg gaaaaatatt ttaatgaaga agaattcact cctgaagaga tagacaaagc
     9361 ccttcattca ggtgtattag atggtagtat tgtacctgtt ttatgtggtt cagccatcga
     9421 tactttaggc gtgcgtgtat tacttaattc tatccttagt tatatgccat cacctgctga
     9481 agcaaaatct tcagtagaag gaaagaatcc gaaaacaggt gaaacagaaa caagaaaagt
     9541 agatgaaaac gagccaacct ctgtatttgt atttaaaacc atcgtggatc cttatattgg
     9601 acgtctttct atttttaaag tatgctctgg tgttttaaaa gcagacagtg tattgctcaa
     9661 tgggaaaaaa gatgttgaag aaaaacttag tcacatttat gtattaaaag gtaaagaaca
     9721 aattgaagta tcccaattat gtgcaggaga tatcggggca gttgcaaaac tacaagaaac
     9781 tatgacagga gatactttat ccgaccttaa aaaaccgatt gtttatccgg aaatagagtt
     9841 tcctgaatcc ttgttaatga tggctgtgtt ccccaaaggc aaaggtgacg aagacaaaat
     9901 gtctgcaggt cttaatcgcc ttatggaaga agatccaact ttccgtgtca gcttaaataa
     9961 agaaaccctt gagcaaatta tgtatggtat aggggatcag catttagatg ttattgtcag
    10021 taagttaaaa gcaaaatata aagtagaagt agaattaaag gcacctacta ttccttacag
    10081 agaaactatt aaggctaaag ttaaggttca gggaaaacat aaaaaacaaa caggtggaca
    10141 tggtcaatac ggggatgtat ggatggaatt cgaaccttca ggagacttaa atgtacctta
    10201 tgtatttgaa gaaaaaatat ttggtggagc cgttccaaaa caatatttcc ctgcagttga
    10261 aaaaggtctt caggagtccg ttaaaaacgg ggttttggca ggatatcctg ttgttggatt
    10321 aaaagcaact ttagttgatg gttcttacca tccggtagac tcttctgaaa tggcatttaa
    10381 gatggctacc tctattgcat ttaaagaagg tcttaaaaaa gcaaagcccg ttattcttga
    10441 gccaattgtc agagtaacgg ttacagtacc cgatgaatac atgggagatg ttattggaga
    10501 cttgaataaa cgccgtggta aaattttagg tatgaatcct aaaggcaaat atcaagttgt
    10561 tgatgcagaa gtaccattag ctgaaatgta taagtatgca acggaccttc gttctatgac
    10621 gcaggcaaga ggaagcttta caatggaatt tgacagatat gaagaagcac ccatggatgt
    10681 tcaggaaaaa gtaatagaag caagaaaaaa agaagcttaa tacctccttg catttattaa
    10741 tttgatatga tacaatttcc tcaaaaatat cgataaaagc agtgaagagg actagtaaat
    10801 attcttttga ctttcagaga gctgttggct ggtgcaaaac agtagcaaag agtattgaac
    10861 tcccctcgga gcttcttgct gaaagatata gtaggtgagg acgctttccc cgttaaagga
    10921 atgatataat ggtttatcca ttatatcaat gagtgtattc tccataggag aataaaatag
    10981 agtggtaccg cggaataagc ctttcgtctc taacaggacg aggggctttt atcatttaat
    11041 acaaagatgc tgataatcat taatatagtt attatgagta taggaggaat gaaattgagc
    11101 agcataaaat atgaccataa gaaaattgaa aaaaaatggc gagagatttg ggagaagaat
    11161 cctattaacc aaccagggcc caataaaaag aaatattatt gtttggatat gttcccatat
    11221 ccatcgggta acggtcttca tgtcggacac tggagaggtt atgtattaag tgacgtatgg
    11281 agtcggtata aggtacttca aaattatcat gtccttcatc caatgggttg ggatgctttt
    11341 gggcttcctg cagaaaacga tgccatcaaa aaaggtattc atcctaaaat tggaacagaa
    11401 agaaatgttg ctaattttaa aagacaacta catgatatca gtgcaattta tgattgggac
    11461 agagaaatta atacaacaga tccgaattat tataaatgga cccaatggat ttttgttaaa
    11521 atgtttgaaa aaggattggc ttatgaaaaa gaaatgccta tcaattggtg tcccagttgt
    11581 aaaaccggat tagccaatga agaagttgta ggtggtcgtt gtgaacgctg caattctgtg
    11641 gttacaaaga aaaatttaag acaatggatg ttaaaaatta cggcttatgc ggaaagatta
    11701 cttgaggatt taaaagattt ggactggcct gaaaaagtta aaaagatgca gacagactgg
    11761 attggaaaaa gttacggggc agaaattgat tttcaggtga aagacagcaa caaaaaaata
    11821 aaagtcttta caacgagacc agataccttg tatggagcta cttttatggt tttggcacca
    11881 gaacatgaaa tggttgctga aataaccaca gaagaacaaa aagaaaaggt agaagagtat
    11941 gtacagttgg ctttgacaaa gtcatcggtt gacagaatgg cagataaaga aaaaactgga
    12001 gtctttacag gagcttatgc aattaatcct cttaacggag ctcaaattcc tatttggatt
    12061 tcagattatg tattagctga ttatggaaca ggggctatta tgtgtgttcc agcccatgat
    12121 gaaagagact tcctctttgc taaaaagttt gatcttccaa ttattcaggt aatcaaaaaa
    12181 gaaggggaag aagaacagga attaacagaa gcttatactg aagaaggtat tatgattaat
    12241 tctgaaaact ttaatggtat tccttccagt gaagctaaag aacgtattgc agattacctt
    12301 gaagaacaag gtattggcaa aaaaaccgta aattataaat taagagactg ggtattttca
    12361 aggcaaagat attggggaga acccattccc attgttcatt gtgaaaaatg tggtgctgtt
    12421 gcggtaccag aagaagaact tcctgttacc ttgccagaag tagagtctta tgagccaaca
    12481 ggtacagggg aatctccttt agcagccatt aaggaatggg tcaatacgac atgtcctaaa
    12541 tgtggtggcc ccgctaaaag agaaactaat acaatgccac agtgggcagg ttcttcatgg
    12601 tatttccttc gttatgctga tcctcataac gacaaagaat taattagtaa ggaagcaatg
    12661 aaaaaatggt tgcctgttga tatgtatgta ggaggcattg agcatgcggt actgcacctg
    12721 ctttatgcaa gattttatac aaaattctta tatgatattg gcgtagtaga tttccaggag
    12781 ccctttatac gcttgtttaa tcagggaatg attacaaaag acggtgcaaa aatgagtaaa
    12841 tctaagggta atgttgtatc acctgatgaa ttggtagaaa aatacggtgc ggattccctc
    12901 agaatgtatg aattatttgt aggtcctcct gaattggatt ctgaatggga tgacagaggc
    12961 attgatggtg tatatcgctt tattaataaa gtatggaagc tgattacaga aaataaagat
    13021 gccaatgtag aagcaacaca ggaaatggaa agagttcgtc ataagctcat ttatgaaatt
    13081 acaacaagaa tgaatgattt ccactttaat accgttgtaa gcggattcat ggaatatacc
    13141 aataaattaa ccgaaatcgc aaaggcttcc aatggtttag acaaagaaac cattgaaacg
    13201 cttattatac ttttagcacc ttttattcct catgcggcgg aagagctttg ggaacttaca
    13261 ggacatcatg aatctgtatt taagcaacaa tggccttctt atgatgaaga aaaaatgaaa
    13321 gaagatacga ttgaaattgt tattcaaatt aacggaaagg ttaaagataa aatagaaatt
    13381 aggattgatg aagctaaaga aagcgtcctg gaaaaagcta aagaggcagt taagaataag
    13441 atggatggta aaagcattgt taaggaaatc tatgtgccag gtaaaattgt gaatttagtt
    13501 gtaaagtaaa aatataaatt tgtggatcag gtgattcatg gaaaagtatg cttgtgaaag
    13561 tctttttctg gtcaggcgtc tgattttcat tcagacgcct gattcaggat aagatgttgc
    13621 aggcatactt tttttatgaa tacatatttt aaataaacat tgctcacaat aataaagaat
    13681 ttatcttaag gaggcaatgt tgatgaaagt aagagatatt atgactcaaa atgttatttc
    13741 tgtagatgaa gaagaatctg ttttaagagc ttctcaagtc atgagagatg ccaatgttgg
    13801 gtcagtacct gttgtcaggg gagataagct ggtaggaatt attactgata gagatattgt
    13861 agttcgtaat gttgccgatg gtgaagatgt ggataatgtt acatgcaaaa atattatgac
    13921 cacagattta gtaacttgtt ctccagacat ggatgttgat gaagtagcag ctttaatgtc
    13981 aaaacatcaa gtgagaagaa tcccggttgt tgaaaatgga aaagttgtag ggatggtttc
    14041 tttaggtgat ttggcaacac aaagaactac tcaggatgaa gcagaagaag cattaaaaaa
    14101 tatatccagt ccaagtatgc ctgaatatca ataaaaggta tgggttcccc atacctttta
    14161 atgctttaac ctgacataag gagtgaatgg atcgctatga aaaataataa aataattatt
    14221 tgtgttctta ttcttttttc cttagtgact acacctcttt tatattatat cagtgacaat
    14281 cccggactca ttcaaggtaa gttgattcat aacagtatgc taaataaacc tggaggaaag
    14341 tcaaaggtta tctatgattt aaatgtgcct ttagataacc atttaattag tcgaatgaaa
    14401 atttatgaag caaaaactaa agagatagaa gaacaagaaa ttaaacacta tgttaaggtg
    14461 cttaaattaa actttagtga tataaaagaa gatgtttcag cttataggat acaaaatgat
    14521 cattatcaac tgattgtgga taaaaatacg ggtcttatac aatataaaaa tttggaaatc
    14581 aatattgaag ataacaataa aaaaatcaac ccggaagaag ccattgaaat ttttaaggct
    14641 tttgccgaaa agttaaatct tccgcttaat taccgcaaat gcctggtaga agatattcca
    14701 gataaaaaac tttatcagct tcgttttata aattcaatcg aagggatttt aaattatggt
    14761 tattgtacca aggctgaact aagccataag ggagaaattt tgaattttaa ttattatcaa
    14821 ctggtctata agccacgtca aattgtatcc attaaaacaa aaaaagaagc ttatgaggat
    14881 ttattaaaaa ttccttttga agaagaagcg catattgata tacaaaaaat tgaattggta
    14941 tattttacgc aggatataga aaaaaagaat agggaaaaat ttgaagatga gtttatatta
    15001 ataccatgct ataggttttt tggagaaatt agcaaaaata agtcttttga atattttatt
    15061 cccgccctga aggaatagag tatttttatt tactgtattg acagttatcc ctttcatcaa
    15121 tcataatata agaggatatc attgtaatta ttataggagg agaattgtag tgtcaaagaa
    15181 agaaacagaa tttgatcgct atgaaagaat gaaaaaaatc caaagggaaa tggaaatcca
    15241 aaaaagaaaa gagatggatg attttagggc gggtaaaaac aaaaagaaga aaaagatggg
    15301 tttttcagag aataatacta gaaggaaaga ttggactaaa tattatgagg ctgatatgga
    15361 tgattatgat gaagtatatt aaattttatt aagttaaaat agattattat aaaaaagcta
    15421 tagattctat agctttttta tgtgtttaaa attttcatca tctgaatgat ggcttcaaat
    15481 ttttttcttt gctctgaggg aagactttct ttcattgctt ctaaaatggc ttcattttgt
    15541 tctctggtca gttttttgcc attttgtttt ctttgagccg ctaattttat taaagcttct
    15601 aagatttcct gatcagattt tcccttaaga ctattcatga catctgttgc ttcttttatc
    15661 atatcggggt ctaaatcttt taaaatatct cttttcatat tataacctcc aatttattat
    15721 ttattctatt aaactggaaa ataaattaag catttgtttt tgattatcat cgagtaaggg
    15781 ggacaggctt tcaattaatt tttgttttgt attctttttt tccgtagttt ctgggatttt
    15841 ttttacttcc tcagcaattt ttaagctttg gatttttttc ataagttctc caatatccat
    15901 gaccttaacc atgatgtcta tcagatattg gttttcatgg gaacaatgag gtttaaaaga
    15961 atttaacatt tccgttttcc aataaggatt ggattttaga ttcatgttta tcaatggatt
    16021 ttcgggattt ttataataca tatcaatgac tcttttaaat tccatggctt taacgaaaac
    16081 gccaaggatt ttttgatgat aaggctcaaa atagggaatg gcggctttga caacttttag
    16141 ttctggtgta tgtatggttt catcaaataa aatagttgca tttggagata ttacaggact
    16201 ggggtttgta actggaacag aaacagcttt attctcttct gaggctttat gattcattaa
    16261 actggaaaac aactttgcca tttcaagcat ttttgaaata tcatatccgt tattttcttg
    16321 ctcttgattg gtttccggag gagcttgatt ttcgtcacgc attcttatgc ctccttaaac
    16381 aggtttatca tacaacaata ttaattaata tttatgtgtg aatcggttaa aagatgacaa
    16441 gttattgtag tctctaaatt tattctatga aggataagct gaaaatgttc gctgtcttaa
    16501 aaaagaggaa ttaacaaatt tttattttaa atcatacact attatagata ggcgtaattt
    16561 tgaagctgag gagggaagca taagaatgga ttatttactt aaggatattg aaagtctatt
    16621 gggaactcat atagatttta aggaagatat ccggcaaata agagagagca ttataaattt
    16681 gttcaaagaa aaatatttta ataaaataca gtcccttatt gaggaaaata gaatacaagc
    16741 ccaggaaaat cctacgaaag aaatgacttt acttaaagcc ctgaaacctt ttatggattc
    16801 aaaaaatcac cagatgcttg atgaaataga aaaatttatg aatacaatca gggtgcttga
    16861 gaatgtcaat cagaagttaa aaagtttaca caggcaaagt aatgacgacg attcaaaaga
    16921 agaaaaaaaa gaaaaaatta tagtcgatca ggatggggtt tatgaaattg atgaagcttg
    16981 cataaataat attcaaagag agtcaaaagg aaaagaaata cttcttttat ttttaattat
    17041 tttactttta aaacgctgaa aaataaaaag cagaggaaag cctctgcttt ttatttttta
    17101 acaaactccc ccaccataac cgccaaacag aatcaagaag atgattaaga aaaagaataa
    17161 tgaattatca gcgaagtcac caaatccgcc ctgacctttt gattcatatc cgaaaagtcc
    17221 gcctccgtta ttaaaaagta gcaggaagaa gaacaggaat aaaagagtat tggttccacc
    17281 gcagtatgat ttagaatcag ccataaagat gcctccttat ttataaataa tat
//
LOCUS       DAOSUD010000044        17168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2063_length_17168_cov_25.057091, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000044 DAOSUD010000000
VERSION     DAOSUD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17168
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_17168_cov_25.057091"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLG49_06900"
     CDS             <1..734
                     /locus_tag="PLG49_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17083.1"
                     /translation="KGNFPLNSYIYLGEDNINYQQRLIPHNTLLGIYAQQGIIGVSIF
                     LIFPGYLLYKLLKTKRSQNKYVIPLLVGFLTQSFAINIENVRFMWFLFGTLLASCELN
                     IDITISEQEKWWNNREYKLFNYSLLFLIIVLFINISRYFHVNILAYNGKVIERTVRLP
                     EEGIYTVAFDIRTNDTENKVEIYDGDKLLKSIVFKSAKGKVEEQIALSHNVTVKFISN
                     NDGWMKIRNAYISNKKILFLFILIH"
     gene            845..1576
                     /locus_tag="PLG49_06905"
     CDS             845..1576
                     /locus_tag="PLG49_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17084.1"
                     /translation="MEISNVTINKYSNLTLLFDIELEYKKEIDFDCNLSLRLKYNSIS
                     DLLPYEPQRNIISYNIPVYRKNSERKLGDKYSAKKYQLLSSTDFKLYGQFYDVNNKAL
                     YENAFFEIPFGFIEKEQDIINPGKSYWVNLLYRVTEDNKINMSYSGWVETKRYRLEPG
                     EYNITFKAQGSLLDDEYSVIRIRDSYLNEIAVINLDGTNREYSVPYKVTQKEEGISFI
                     LDLINFERKDNKDRRVILEDWVKIE"
     gene            1719..3575
                     /locus_tag="PLG49_06910"
     CDS             1719..3575
                     /locus_tag="PLG49_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOQ17085.1"
                     /translation="MKQRYSAWILALADIILINLSIIFAYLLRFGGYIHPGYFAIYKN
                     MAIAVTGIKIVIFYYFGLYKSLWKYASINELISIVVATAMGNAAVLAYTYLTQIPNFQ
                     EAILPVPRAIYIIAWLLDIMFIGSIRFAYRVLKQWSFKGIWQPSDKKRALIIGAGDAG
                     AMVIREMHNHEELGMYPVAVIDDDDLKLGKKIHNVPVVGTRYRIEEIVIKKGIDIIII
                     AIPSINKKDRREIIEICNRTGVEVKILPGVYEFIEGNINISHLRNVEIEDLLGRDPIE
                     LDVDQISGYLKKSVVLVTGGGGSIGSELCRQITAFNPKKLLILDIYENNAYDIQNELL
                     EKYPSMDLTVLIASIREKERIDEIFNEYRPQIVFHAAAHKHVPLMEHNPMEAIKNNVF
                     GTLNLVQAADKYRVKRFVLISTDKAVNPTNVMGASKRLCEMMIQTYNRYSKTEFVAVR
                     FGNVLGSNGSVIPLFKKQIEKGGPATVTHPDIIRYFMTIPEAVQLVIQAGGMAKGGEI
                     FVLDMGEPVKIDKLARDLIRLSGFEPDVDIKIVYTGLRPGEKLYEELLMEEEGLTETI
                     HDKIHIARPMDLTMEELNEKLDILSRACYGKKDEVIKAIKETVPTYNPVNND"
     gene            3746..4735
                     /gene="galE"
                     /locus_tag="PLG49_06915"
     CDS             3746..4735
                     /gene="galE"
                     /locus_tag="PLG49_06915"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ17086.1"
                     /translation="MTILVAGSAGYIGSHTCVVLLEAGYDLVVADNLSNSRKETIDRI
                     KKITGKDFVFYKIDLRDVEKVEELFINHHLDGVMHFAGYKAVGESVYKPLDYYYNNLV
                     TTMVLSMACIKHKVNKFIFSSSATVYGNNKVPFVETMDLLPATNPYGESKAMNEKILM
                     DTAKAYPDFSVSILRYFNPIGAHESGLIGELPKGIPNNLMPYITQVAKGKLEKLKIFG
                     NDYPTIDGTGVRDYIHVMDLAEGHVAALEKIRPGIHVYNLGTGQGTSVLQLINTFKEV
                     NGIDIPYEIVGRRAGDIPISYADVSKARKELGWTAKRGLKEMLRDAWNFEKRL"
     gene            4762..5079
                     /locus_tag="PLG49_06920"
     CDS             4762..5079
                     /locus_tag="PLG49_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOQ17087.1"
                     /translation="MEQEISLRELIEVLLKNKKLIAIITGVSLIFSLILSFTTPPVYE
                     ARTTLLVNPIGAQQKSKEITSSMDVLDTISQYPEISVQTYKEQFLSAEVLKGTIDELG
                     LKK"
     gene            5186..6145
                     /locus_tag="PLG49_06925"
     CDS             5186..6145
                     /locus_tag="PLG49_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOQ17088.1"
                     /translation="MAAEIANTLNEKFIDYITAMTKRKSMQAVQAITEQLEEEKKALD
                     EEAKKKRDYLMNAQDIEALNQEIEALRNQLTAYKSLLIDTEKQIESDLLSLKTMEQNG
                     IGSNVDIAAIEAKIQLKNEVKNELGQLAGTQTSTNNNANGNDNGNGDNNLINNSSSND
                     SVIEQAIEIKLSNSSNLSNAILTAQKTQIETRLVQNVSEKEVLEKKIEEIQQRLSDLR
                     ATLATEEYKYNEVMRNYELAEASFEAYQIKKNEAEQNAAADIGRASIIVSSPAVVPEV
                     PSSPNKKLNLGIGIVLGLMIGVLVAFFREYWKNSAVEATKKSS"
     gene            complement(6164..6457)
                     /locus_tag="PLG49_06930"
     CDS             complement(6164..6457)
                     /locus_tag="PLG49_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17089.1"
                     /translation="MLTCPKCQNKELIIRREVSYVYSYKIDTDEESIESKLQEYPYLF
                     YNREILDSKDYIQCSKCGAQFPFSFGDNPKESKMTILKKAIRSDRVKNPQFFG"
     gene            complement(6561..10838)
                     /locus_tag="PLG49_06935"
     CDS             complement(6561..10838)
                     /locus_tag="PLG49_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOQ17090.1"
                     /translation="MSNKLHVGLDVGSTTVKIVVIDSENTILFSRYQRHYSDIKQTII
                     NLLTETYRCFPNEHITIGITGSGGLSISEWLNIDFVQEVIAGAKAVETFIPETDVAIE
                     LGGEDAKITYFEGSIEQRMNGTCAGGTGSFIDQMASLLKTDAPGLNELAKNHKTIYPI
                     AARCGVFAKTDVQPLLNEGAAKEDIAASVFQAVVNQTISGLACGKPIRGNVAFLGGPL
                     SFLSELRKRFIETLNLKEDQVFAPENAEYFVALGAAILSKKQKPVSFKALYKRLPGLK
                     EAESHEIQRMRPLFISQEELEAFRKRHEVYRVKRNELSSYTGKCFLGIDAGSTTTKVA
                     LIDEEGALLYSHYGSNEGNPLASVIKVLKDLYSKLPKEATIAYSAVTGYGEALIKAAL
                     KVDIGEIETIAHYKAADFFLPGVNFILDIGGQDMKCLRIKDGVIDKIMLNEACSSGCG
                     SFIETFANSLNMSVSDFAKEALMAEHPVDLGSRCTVFMNSRVKQAQKEGASIGDLSAG
                     LSYSVIKNALFKVINLKSPDEMGDKVIVQGGTFYNDAILRSFELVSGREPVRPDIAGI
                     MGAFGAALIAKEKYKGEETTLLSADRLNVFSTQTTQKRCPLCPNQCHLTVNTFSDGRY
                     FISGNRCERGAGVEVKKEHLPNLFDYKYKRIFSYKPLSEEEAKRGTIGIPRVLNIYEN
                     YPFWFTFFTNLGFRVELSPRSSKKVFERGIETIPSESVCYPAKLTHGHIMSLIEKGIT
                     RIFYPCLPYEKQEDPGANNHYNCPIVTSYPEVIKNNMEVLKEKGILFMNPFLPIDHKE
                     RLKERLYEEFRIFGISKEEIDRATELAWAEEEKVKKDIRQKGEEVLKYLEENHIKGIV
                     LSGRPYHIDPEIHHGIPDLINALGMAVLTEDSIAYLGQVQRPLRVIDQWVYHSRLYAA
                     AHLVGTKDYLELVQLNSFGCGLDAVTTDQVREILEGHGKIYTVLKIDEGNQLGAARIR
                     LRSLKAAMEERSQNIKAAKKDIRLKRPVFTKEMKKTYTILAPQMAPYQFEFLQEAFCA
                     SGYNLEVLPSVDYAAVDVGLKYVNNDACYPSIIVVGQLISALQSGKYDLDKTAIIITQ
                     TGGGCRATNYIAFLRKALKDANMAHVPVISLNALGLEKNPGFKITPKLLKRAIMALLY
                     GDLLVRVLLRVRPYEKIQGSAELLYKQWAEKCKKAIHRCRHKEFKENVWNIVKDFDHL
                     EIKNQTKPRVGLVGEILVKYHPTANNNVIGLVENEGAEAVLPDLTGFFLYGAYNSTFK
                     YEKLAGSRKEKWAGLALIKMIEYYQKDIIKALEKSKRFEAPKSIYELAEGVEDILSIG
                     NQTGEGWFLTAEMVELIESGVENIICMQPFACLPNHVTGKAMIKPLKQKYPGANIVAV
                     DYDPGASEVNQLNRIKLMLSVAFKNLEKQTREEKIHKKMHHTGAASK"
     gene            11101..13029
                     /locus_tag="PLG49_06940"
     CDS             11101..13029
                     /locus_tag="PLG49_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HOQ17091.1"
                     /translation="MDAAKFYKIIQKDREERKGANFKGNFLDYLEIVKENPEVAVLAH
                     ERIYKLLIEPGVEVIKTEEDPRLRRIYGNDIIKRYNFFKEDFFGIDRVLMKIVRYFHS
                     AAMRGEESRQVLYLVGPVGSGKSSIIEAIKKLLESSPPIYALEGCPIREEPLHLIPHH
                     LRDLFEKELKVKIEGDLCPVCSYRLQHEFEGKYENFPVTSVEFSIQGRKGIGVVPPVD
                     SNNQDTSVLIGSVDISKMDLYPEDDPRVFSLNGAFNVGNRGIVEFIEVFKNDVEYLHT
                     MITATQEKAIPSPGKGAMVYFDGVILAHSNESEWNKFKSDHTNEAILDRIVKIEVPYC
                     LELNEEIKIYEKILKKSNFKAHIAPHTLEVASMFAILSRLKPSNKVDPMTKLKIYNGE
                     EIIEKGTTKRIDIAELREEAGKEGMSGISTRFIMKALDNALSDSEHNCINPLSVMEAL
                     IKEVKEMAVSEELKKRYLGFIQDTIKKEYHLILEKEVTRAFIHGYREQAQDLFNNYLD
                     HAEAYINKTKIKDLSTGEELEPDEEFLASIEEQLGITPSSAKGFRQDVTSYMFAVLRN
                     GGKIDYTCYEPLKEAIEKKLTASVRELSRVITKSKVRNEEQNKKYDAMVEEMKKNGYC
                     DHCCNVILKYSANNLWKD"
     gene            13046..14209
                     /locus_tag="PLG49_06945"
     CDS             13046..14209
                     /locus_tag="PLG49_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HOQ17092.1"
                     /translation="MAIFREYQKYGRDRSIEDRRRHKKLVEKSIKENIGRIISEESII
                     GQSNNKKIKIPIRGLKEYYFRYGSNQKEIFSGDGKEKKGDKYLINKYKFGKEEGRAGN
                     EEGEDIYETEITIEEAIQYLFEDINLPYLYRKEFTETESESTGKKWGIQVKGAPPRLS
                     KKHTVKERIKRKKQAQRNQVIKEGDRFPFHEEDLRYYRLKPKKKRDSNGVVICIMDTS
                     GSMGQTKKYLARSFYFLLYQFLKIKYLNVEVVFIAHTTVAKEVSEDEFFHKAESGGTY
                     ISSGYKKALEIIHERYNPKTWNIYAFHCSDGDNWGEDNQRAVEAALELCKVCNLFGYG
                     EIKTSTYTSTIMQKYMEEIKEPNFAMVKIAGKEDVWAAFVELLNVEKNNRLEG"
     gene            14214..15530
                     /locus_tag="PLG49_06950"
     CDS             14214..15530
                     /locus_tag="PLG49_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOQ17093.1"
                     /translation="MGYSMDYSIEELKFWNEKIERLAEEKGLDFYPQEFEIISYEDML
                     GYESYLGMPSHYPHWSFGKTYERLKTLYKYNLTGLPYEMVINSNPSIAYLMKDNTLVL
                     QILTMAHVYAHNDFFKNNRLFQEYTNADLAVEMFKNHAGRIREYIKNPSIGYEKVESV
                     LDAVHSIRYQCGLKKRKKYEKTIEHQEEEEVEKKESDSLLQFLIDYGDCEEWEKDIIY
                     IVMEETRYFIPQIESKIINEGWACWWHYQLLNDLDLDYGSHIDFLRIHNNVISQYPGR
                     INPYYLGFKIWDKIAKKTRDMKKLFEIRALERDSSFIRRYLDFDTCREMNLIQLEEQD
                     RFYVITELADDEGWKSIRNYLAESVGMGRIPKIVVDDVIKSDNLLCLKQIYDGRELDV
                     TYAFETLKYIVKLWGGKVSLDTVINKSPKRIICDKEFRISLENIED"
     gene            15621..16424
                     /locus_tag="PLG49_06955"
     CDS             15621..16424
                     /locus_tag="PLG49_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ17094.1"
                     /translation="MGFKFCSITSGSSGNCIYIGTPKVNILVDAGISGTKVQAGLEKI
                     GVNPASLHGILITHEHIDHIKGVGILSRRFDLPLLATAKTWEAMEAMGKVLGKIAPHN
                     KVVIEKDKDFILKDLVIHPYPIPHDAADPVGYTFVHSNKKISIATDLGHVNDQIKDNI
                     RESNLILLESNHDIDMLINGSYPYYLKRRILSNFGHLCNEAAGKILAEVFHDKLEAVI
                     LGHLSKENNYPQLALNSVKKQLLSAGIEQDGRFELTVAKREDVSEFFSL"
     gene            16519..16659
                     /locus_tag="PLG49_06960"
     CDS             16519..16659
                     /locus_tag="PLG49_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17095.1"
                     /translation="MAKKKKIDKASQPASFATEEFASESIPQQGSQKSRKKSGKEKKE
                     IV"
     gene            16856..>17168
                     /locus_tag="PLG49_06965"
     CDS             16856..>17168
                     /locus_tag="PLG49_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOQ17096.1"
                     /translation="MRLSSHVFVADYVANILEELVGVKFNRRFLILGAKMPDIQPIRR
                     MEIHSPQVILPHFEREYDRIVLEEKKINRISYILGLLTHYISDGFCYSHNVYTIDMKK
                     HI"
BASE COUNT         5769 a         2634 c         3439 g         5326 t
ORIGIN      
        1 gcaaaggaaa ttttccattg aactcctata tctatttagg tgaagataat attaattatc
       61 aacagcgttt aatacctcat aatactcttc ttgggatata tgcacagcaa ggcattattg
      121 gagtatccat ttttttaatt tttccaggtt atttacttta taaattatta aaaacgaaaa
      181 gaagtcagaa taaatacgta attccattat tggtaggatt tttaacacag tcttttgcca
      241 taaatattga gaatgtaagg tttatgtggt ttttatttgg tactttgctc gctagttgtg
      301 aattaaatat tgatataacg attagcgaac aagaaaaatg gtggaataat agagaataca
      361 agttgtttaa ttattcacta ttatttctta taatagtctt atttataaat attagtagat
      421 attttcatgt taatatatta gcttacaatg gtaaagtaat agagagaacc gtgcgattac
      481 cagaagaggg catttatact gtcgcttttg acatacggac aaatgatacc gaaaacaaag
      541 ttgaaattta tgacggtgat aaattattaa agtctattgt ttttaaatct gccaaaggta
      601 aggtagaaga gcaaatagcg ttaagccata atgttacagt aaaatttatt agtaataatg
      661 atggttggat gaaaatcaga aatgcgtata tcagtaataa aaaaatacta ttcctcttta
      721 ttcttatcca ttagtaccag atattttaat aagtagttta aatagaaaaa atctgcttgt
      781 atatacgaaa gaatcttcct taaatcagga aataaatact ttaagcaaaa ataactttga
      841 atccttggaa atatcaaatg ttacaataaa taaatattca aatcttactt tattgtttga
      901 tatagaatta gaatataaaa aagaaattga ttttgattgc aatctaagtt tgagactaaa
      961 atacaattct atttctgatt tattaccgta tgaacctcaa cggaatatta tctcgtataa
     1021 tattccagtg tatcgaaaaa attcagaaag aaagctagga gataaatatt cagcaaagaa
     1081 atatcaatta ttatcttcga ctgactttaa actgtatgga cagttttacg atgtcaataa
     1141 taaagcactc tatgaaaatg catttttcga aataccattt ggctttattg aaaaagaaca
     1201 agatattatt aatcctggaa aaagttattg ggttaatctt ttatatcgag tgacagaaga
     1261 caataaaatc aatatgagct atagtggatg ggtagaaacc aaaagataca gactggaacc
     1321 tggagaatat aatattactt ttaaagctca aggttcattg ttagatgatg aatattcagt
     1381 tatccgtatc cgggacagct accttaatga aattgcagtt ataaaccttg atggtacgaa
     1441 tagggaatat tctgttccat ataaagttac tcaaaaggaa gaaggcataa gctttatttt
     1501 agatttgata aactttgaaa gaaaagacaa caaggacaga agggttattt tagaagactg
     1561 ggtaaagatt gaataataaa atttatattg ggattgggta aaaattattt tatattagtt
     1621 aatcataatt agatgaagat tcagtttatg actgtaaaat tgacattttc tattgttgag
     1681 gttatcatat ttgtagttta ttagagagga agtcacggat gaaacaacgt tacagtgcct
     1741 ggatacttgc tttagcagat attattttaa tcaatttatc tatcattttt gcatatttac
     1801 tcaggtttgg cggatatatt catccgggct attttgccat ttataaaaat atggctattg
     1861 ccgttacggg tattaagata gtaatatttt attattttgg tttatataaa agcctatgga
     1921 agtatgccag tattaatgag ctcatttcta ttgttgtagc aactgccatg ggaaatgcag
     1981 ctgttttagc ttatacctat ttaacacaaa ttccaaattt ccaggaagcc attttgcctg
     2041 ttccaagagc aatttatatt attgcctggt tgctggacat tatgtttatt ggaagtatcc
     2101 gttttgctta ccgtgtttta aaacagtgga gttttaaggg aatttggcaa ccttctgata
     2161 aaaaaagagc ccttattatt ggtgctggag atgcaggggc aatggttatc cgtgagatgc
     2221 ataaccatga agaattgggg atgtatcccg tggctgtcat tgatgacgat gatttaaaac
     2281 ttgggaagaa gattcataat gtgcctgttg taggaaccag atatagaata gaagaaattg
     2341 taatcaagaa aggtatagat attatcatta ttgctatacc ttccattaat aaaaaggatc
     2401 gtagggaaat tatcgaaatc tgtaaccgga ctggagtaga agttaaaatt ctgccaggag
     2461 tttatgaatt tattgaaggc aatattaata ttagccatct tcgaaatgtt gaaattgaag
     2521 acttattggg aagagatccg atagaactgg atgtcgacca gatcagtggc tatctgaaaa
     2581 aatcagtggt tttagtgact ggcggtggtg gttctattgg ttctgaatta tgccgtcaga
     2641 ttacagcctt taatcctaaa aaactcttga ttcttgatat ttatgagaac aatgcttatg
     2701 atatacaaaa tgaattatta gaaaagtatc cctctatgga tttgacagtg cttattgctt
     2761 ctatcaggga aaaggaaaga attgatgaga tttttaatga atacagacca caaattgttt
     2821 tccatgcggc ggctcataaa catgttcctt taatggagca taatccaatg gaagcgatta
     2881 agaataatgt ttttggtact cttaatttag tacaagctgc tgataaatat agggtaaaac
     2941 gttttgtttt aatttctacg gacaaagcgg ttaatccgac aaacgtcatg ggtgccagca
     3001 agagattatg tgaaatgatg attcaaactt acaacagata ttcaaaaaca gaattcgttg
     3061 cagtgcgttt tggtaatgtt ttaggcagta acggcagtgt gatcccttta tttaaaaagc
     3121 agattgaaaa gggaggacct gcaacggtta cgcatccgga tataatcaga tattttatga
     3181 cgattcctga agctgttcag cttgttatcc aggctggagg gatggcaaag ggtggagaaa
     3241 tttttgttct ggatatgggg gaacctgtta aaattgataa gttagccagg gatttaatca
     3301 ggctttctgg ttttgaaccc gatgtagata taaagatagt ttatacaggc cttcggcctg
     3361 gagaaaagtt atatgaagag ctcctgatgg aagaagaagg tttaactgaa accattcatg
     3421 ataaaattca tatcgcaagg cctatggatt taaccatgga agagctcaat gaaaaattag
     3481 atattctaag cagggcatgt tatggtaaaa aagatgaagt gataaaagca ataaaagaga
     3541 ctgtacccac ttataatcct gttaacaatg attaacacat accctttttc atgcttgcta
     3601 tgtatgaaaa agggcttttt aaaaaagata taaagaagag atagagtaag tttttgcctt
     3661 taataagaga gtttttttta tgtttatctt ctgttataat aagatagatt gaaaaatgaa
     3721 tttcattttt aaaggtggac aaattatgac tatattagtt gccggtagtg ctggctatat
     3781 cggttctcat acttgtgttg ttctgcttga agccgggtat gatttggtgg tagcggataa
     3841 tttatcgaac agcaggaaag aaacaattga cagaattaaa aaaataacag gaaaggattt
     3901 tgtcttttac aagattgatt taagggatgt tgaaaaagtt gaggaattat ttattaatca
     3961 tcatttagac ggtgttatgc attttgcagg ttataaagcg gtaggagagt cggtatataa
     4021 acctttggac tattattata acaatcttgt aacgaccatg gttttatcca tggcttgtat
     4081 taagcataaa gtcaataagt ttatttttag ttcatcagca accgtttacg gcaataataa
     4141 ggttcctttt gttgagacga tggatttgct gccagcaacg aatccttatg gagaaagtaa
     4201 ggcgatgaat gagaagattc tcatggatac tgcaaaggca tatcctgatt tttcagtttc
     4261 aattcttagg tattttaatc ctataggagc ccatgaaagt ggtttaattg gggaattacc
     4321 taagggaatt cccaataact taatgcctta tatcacacag gttgctaaag gtaaactgga
     4381 aaaacttaag atctttggca atgattatcc aaccatagat gggactggcg tgagggatta
     4441 tattcatgta atggatctgg cagaaggtca tgtagcagct ttagaaaaaa taagaccagg
     4501 gattcatgtt tataatcttg gtacaggtca gggaacttct gtattgcaac tgattaatac
     4561 ttttaaagaa gtcaatggaa tagatattcc ctatgaaatt gtgggaagaa gagctggaga
     4621 tattcccata tcttatgcag atgtcagtaa agccagaaaa gaactgggtt ggacagccaa
     4681 acgtggtctt aaggaaatgc taagagatgc ctggaatttt gaaaaacgtt tatagagatt
     4741 gttaggaaat ggagggtaaa gatggagcaa gaaattagtt taagagaact gatagaagtt
     4801 ttattaaaaa ataaaaaact tattgctatt attacaggag tatcattaat attttcgctt
     4861 attttaagtt ttacaactcc acctgtatat gaagccagga cgactctttt ggttaatcct
     4921 ataggtgctc agcaaaagag taaggaaatt accagcagta tggatgtatt agataccatt
     4981 agccagtatc ctgaaatttc tgtacaaact tataaagaac agtttttaag tgctgaagtt
     5041 ttaaaaggaa cgattgatga gttaggttta aaaaagtgaa gatgggcagg caatatctat
     5101 tcgtagctta aggaataagg ttactgtaga ggttattgat aatacgaatt taattaaagt
     5161 aacagtaaga gatggaaatc ctcaattggc agccgaaatt gccaatacgc ttaatgaaaa
     5221 atttattgat tatattacag ccatgacaaa aagaaagagt atgcaggcag tacaggcaat
     5281 aacggagcag ttggaagagg aaaaaaaggc tctggatgag gaagctaaga aaaagagaga
     5341 ttatttaatg aatgcccagg atattgaagc cctcaatcag gaaattgaag ccttaagaaa
     5401 ccaattgaca gcttataaga gtttactgat agatacagaa aagcaaattg aatcagattt
     5461 attatcctta aagacaatgg aacaaaacgg aataggttca aatgtcgata ttgcagccat
     5521 tgaagcaaaa atccagttaa aaaatgaggt aaaaaatgaa cttggacagc ttgcaggaac
     5581 acagaccagt acaaataata atgccaatgg aaatgataat ggtaatggag ataataattt
     5641 gattaataat tcttcttcta atgatagtgt tatagagcag gcaattgaaa ttaaacttag
     5701 caatagttct aatttatcta atgcaattct gaccgcacaa aaaacccaga tcgaaaccag
     5761 actggttcaa aatgtttctg aaaaagaggt actggaaaag aaaattgaag agattcagca
     5821 aagattgtct gatttaagag caactttagc gacggaagaa tataagtaca atgaagttat
     5881 gagaaattat gaattagcag aggcttcttt tgaagcttat caaataaaaa agaatgaagc
     5941 agagcaaaat gctgctgctg atatcggtcg ggcaagtatt attgtttctt ctcctgctgt
     6001 agtgccagaa gtgccttcta gtccgaataa aaaactaaac cttggaattg ggattgtttt
     6061 aggcttaatg attggcgtat tggtagcttt ctttagggag tactggaaaa acagtgcggt
     6121 agaagccact aaaaaatcct cctaacagga ggattttttt attttatcca aagaattggg
     6181 gatttttgac cctgtctgaa cggatggctt tttttaagat ggtcatttta gattctttgg
     6241 ggttatcacc aaaggaaaaa ggaaattgtg cgccacattt actgcactgg atgtaatctt
     6301 tagaatccag gatttcgcgg ttataaaata aatagggata ttcttgtaat ttggattcga
     6361 tggattcttc atcggtatct attttataag agtaaacata gctgacttcc ctgcggataa
     6421 tcaattcttt gttttgacat ttaggacaag taagcatcgg catcctccta aaaaatttca
     6481 tgtttttatt atctcttagt acttaaaaaa tatgcaaaaa agaggctgct tagaatgatt
     6541 ctaagagcct cttttatttt ttattttgaa gcagcacctg tatggtgcat ttttttatgg
     6601 attttttctt cccgtgtttg cttttccagg tttttaaaag caacagaaag cattaattta
     6661 attcggttca actgattaac ttcgctggca ccagggtcat aatctacagc gacgatattt
     6721 gcccctgggt atttttgctt taatggtttt atcatggctt tacccgttac atggttaggc
     6781 agacaggcaa aaggttgcat acagataatg ttttcgactc ctgactcgat aagttctacc
     6841 atttcagctg ttaagaacca gccttctcca gtttgatttc ctatagaaag gatgtcttct
     6901 acgccttctg ccaattcata gatggacttg ggtgcttcaa agcgcttact tttttctaag
     6961 gctttaatga tgtctttttg atagtattca atcattttaa ttaaagcaag cccagcccat
     7021 ttttctttac ggctgccagc aagtttttca tatttaaaag ttgagttata agcaccgtac
     7081 aggaaaaagc cggtcaaatc tggcagtacg gcttcagcgc cttcattttc aaccagacca
     7141 atgacattat tattggcggt tgggtgatat ttaactaaga tttcaccgac taagccaact
     7201 cttggcttag tctgattttt aatttctaaa tggtcaaagt ctttaacgat attccatacg
     7261 ttttctttaa attctttatg acggcaccta tggatggctt ttttacattt ctctgcccac
     7321 tgtttgtaga ggagttcagc agaaccttgt attttttcat aaggtcttac cctaagcagg
     7381 actcttacca gtaaatcgcc gtacagtagc gccataatgg cacgtttaag caatttaggt
     7441 gtgattttaa agccaggatt tttttctaaa cctaaagcat tcagggaaat aacaggcaca
     7501 tgggccatat tggcatcttt taatgctttt ctgaggaaag caatatagtt ggtagcccgg
     7561 cagcctccac ccgtttgtgt aatgatgatg gctgttttat ctaaatcata ttttccggat
     7621 tgtaaagcag aaatcagttg tcctacgaca ataatagaag gatagcaggc atcattattc
     7681 acgtatttaa gacctacatc aactgctgca taatccacgg aaggcagtac ttccagatta
     7741 taacctgagg cacaaaaagc ttcctggaga aattcaaatt gataaggtgc catttgaggc
     7801 gccaaaatgg tataagtctt tttcatttct tttgtaaaaa caggtctttt gagccggatg
     7861 tctttttttg cggcttttat attctggctt ctttcttcca tggctgcctt aagggaacgc
     7921 agcctgatac gggcagcccc cagttggtta ccttcatcaa tttttaagac ggtataaatt
     7981 tttccgtgtc cttctaagat ttcccgtacc tggtctgtag tcacggcatc taaaccacag
     8041 ccaaaggaat tgagttgaac cagttctaaa tagtcttttg ttcctactaa gtgagccgca
     8101 gcatacaaac ggctatgata tacccattgg tcgataaccc ttaaaggtct ttggacctga
     8161 cctaggtagg cgatggaatc ttccgttaaa acggccatgc ctaaggcgtt aatcaaatcg
     8221 ggtataccgt ggtggatttc agggtcaata tgatatggtc tgccactaag aacaatccct
     8281 tttatatggt tttcttccag gtatttaagg acttcttcgc ctttttgccg gatgtctttt
     8341 ttcacttttt cttcttctgc ccatgccagt tcagtggccc gatcaatttc ttcttttgaa
     8401 atgccaaaga ttctaaattc ctcgtatagt ctttctttta gtctttcctt atggtcgatg
     8461 ggcaagaaag gattcataaa aaggatgcct ttttctttta aaacttccat gttattttta
     8521 atcacttcag gataggaagt aactattgga cagttataat gattgttagc tcctggatct
     8581 tcttgttttt catagggcag gcaaggataa aaaatccttg ttatgccttt ttcgattaaa
     8641 ctcatgatat gtccatgagt cagttttgca gggtagcaaa ccgattcaga aggtatggtt
     8701 tcaatacctc tttcaaaaac tttctttgaa cttcggggag ataattctac cctgaaacct
     8761 aagtttgtaa aaaaggtaaa ccaaaatgga taattttcat aaatgttaag tacccgtggg
     8821 ataccaattg tgcctctttt agcttcttct tctgaaagag gtttatagga aaagattctt
     8881 ttatatttat aatcaaacag gtttggcaga tgctcttttt taacttctac gccagcccct
     8941 ctttcacagc ggttacctga aataaagtag cggccgtctg agaaagtatt gacggtaaga
     9001 tgacattggt tggggcaaag gggacagcgc ttttgtgtcg tttgagttga aaacacattt
     9061 aaacggtcag cggaaagaag cgttgtttct tcgcctttat acttttcttt tgcaattaaa
     9121 gctgctccaa aagcacccat gataccagcg atatcaggtc tgaccggttc tcttcctgat
     9181 acgagttcaa agcttctgag gatggcgtcg ttatagaaag ttcctccctg gacaatgact
     9241 ttatcgccca tttcgtcggg gcttttgaga ttaataacct taaaaagggc atttttgata
     9301 acagaataag acaagcctgc tgataagtct ccaatactgg caccttcttt ttgagcctgt
     9361 tttaccctgg aattcataaa taccgtacag cgcgaaccaa gatcaacggg gtgttcagcc
     9421 ataagggctt ctttagcaaa atcggaaacc gacatattta aggagtttgc aaaggtttca
     9481 ataaaggagc cacagcctga agaacaagct tcatttaaca tgattttgtc aataaccccg
     9541 tctttaatac gcaggcattt catgtcctga ccgccaatgt ctaagataaa gtttacaccg
     9601 ggcaggaaaa agtcggcggc tttataatgg gcaatggttt caatttctcc aatatcaact
     9661 ttaagggcag ctttaattaa agcttcccca taacctgtca cggctgaata ggcaatggtc
     9721 gcctcttttg gcagtttgga atataaatct ttaaggactt ttataacgga tgcaagggga
     9781 tttccttcat tgcttccata atgagaatat aaaagagctc cttcttcatc aattaaggcg
     9841 acttttgtag ttgttgaacc agcatctatg cctaaaaaac attttccggt atagctggac
     9901 agttcatttc gtttgactct atatacttca tgtcttttcc taaaggcttc aagttcttcc
     9961 tggctgataa ataaaggacg catcctttga atttcatggc tttcagcttc ttttagaccc
    10021 ggtaatcttt tatacaaagc tttaaacgat acaggctttt gctttttgga taagatggca
    10081 gctcctaaag caacaaagta ttctgcattt tctggtgcaa aaacctggtc ttcttttaag
    10141 ttaagggttt caataaatcg ctttcttagc tcagataaaa aagaaagagg tccgcctaaa
    10201 aaagcaacat ttcctcttat aggtttgcca caggccaatc cgctgatggt ttggttgaca
    10261 acggcttgaa agacagaagc tgcaatatct tctttagccg caccctcgtt aagaagaggc
    10321 tggacatcgg ttttggcaaa aacaccacac cttgcagcga ttgggtaaat ggttttatga
    10381 ttttttgcta actcgtttaa gcccggggca tcggttttaa gtaagcttgc catctggtca
    10441 ataaaagaac ctgtaccgcc tgcacaagtt ccattcattc tttgctctat agaaccttca
    10501 aaataagtta ttttagcatc ttccccacct aattcgatgg ctacatccgt ttcaggaata
    10561 aaggtttcaa ctgctttggc accggcgata acttcctgga caaagtcaat atttagccat
    10621 tcggaaatgg acaagccccc agaaccagtt atgcctattg taatatgctc attgggaaag
    10681 cagcggtagg tttctgttaa aagattgatg atggtttgtt tgatgtcaga ataatgtctt
    10741 tgataacgtg aaaaaaggat agtgttttca ctatcaatga caacaatttt tacagttgtt
    10801 gaaccaacat ccaaaccaac atgcagttta ttgctcatat aatcctcctt ccttaatcat
    10861 aaatatatta aaaagtctta aacccaaaaa ggcttaagac cttttgttat tctggatgca
    10921 tagctttctt attaacataa taatagataa ttgaattaaa tttgtcaaga attttacttt
    10981 ttattcatat ctgtttactt tttatccata tatttataat agaccttttt aagcgctatt
    11041 ttacttttca taaaaaggat ccttggctaa gacgctttga catataaaag gagggattag
    11101 atggatgcag caaagtttta taagatcata caaaaggaca gggaagaaag aaaaggggca
    11161 aattttaaag gtaatttctt agattattta gaaattgtaa aagaaaatcc agaagttgcc
    11221 gtccttgctc atgaaaggat ttataagctt cttatagaac ctggtgttga agtcattaaa
    11281 actgaagaag atccaagact tcgcagaatc tatggcaatg acataataaa aagatataac
    11341 ttttttaaag aggacttttt tggcatagac agggttctta tgaaaattgt caggtatttt
    11401 cattctgcag ccatgcgggg agaagagtcc aggcaagtct tatatttggt gggaccagta
    11461 ggctcaggta aatcttcaat tattgaagcc attaagaaat tacttgaatc gagcccgccc
    11521 atttatgctc ttgaaggttg tcccataagg gaagaaccgc ttcatcttat tccacatcat
    11581 ttaagagatc tttttgaaaa agaacttaaa gtcaagattg aaggagattt atgtcctgtt
    11641 tgcagctata gattgcagca tgaatttgaa ggtaaatatg aaaattttcc agtgacaagc
    11701 gtggaatttt caattcaggg aagaaaaggc ataggcgtag tgcctcctgt tgattctaat
    11761 aatcaagata cttcagtcct tatcgggagt gtggacatat caaaaatgga cttatatccg
    11821 gaagacgatc ccagagtttt ttccttaaat ggagctttta atgtcggcaa tcggggcatt
    11881 gttgaattta ttgaagtctt caaaaatgat gtggagtatt tacatactat gattacggca
    11941 actcaggaaa aggccattcc ttctcctgga aaaggggcta tggtctattt cgatggggtg
    12001 attttggctc actctaatga atcggaatgg aataaattca aatcagacca taccaatgaa
    12061 gcgattttag acaggattgt aaaaattgaa gtgccttatt gcttagaatt gaatgaagaa
    12121 atcaagatct atgaaaaaat ccttaaaaag tctaatttta aggcccatat tgcaccccat
    12181 actttagaag ttgcttcgat gtttgctatt ttaagcaggc ttaaaccttc caataaagta
    12241 gatcctatga ctaagctgaa aatctacaat ggggaagaaa tcattgaaaa ggggacgacc
    12301 aagaggattg atattgcaga acttagggaa gaagcaggaa aggaaggaat gtcggggatt
    12361 tccacaagat ttattatgaa ggccttagat aatgccttat cggattcaga gcataattgc
    12421 attaatcctt taagtgttat ggaagctctg ataaaagaag ttaaagaaat ggccgtatct
    12481 gaagaattaa agaagagata tcttggtttt attcaggata cgattaaaaa agaataccat
    12541 ttaatcctgg aaaaagaagt caccagggct tttattcatg gttacagaga acaggcgcag
    12601 gacttattta ataattattt ggatcatgca gaggcttata ttaataagac caaaattaag
    12661 gacttaagta caggagaaga attagagcca gacgaagaat ttctggcctc tatagaagaa
    12721 cagctcggta ttacacccag ttcagcaaaa ggctttagac aggatgtaac ttcttatatg
    12781 tttgccgttt taagaaatgg agggaaaatt gattatacct gttatgaacc attaaaagag
    12841 gcaatagaaa agaagcttac agcctctgtc agagaacttt cgagagtaat taccaaatct
    12901 aaggtaagaa acgaagaaca aaataaaaag tatgatgcca tggtagagga aatgaaaaaa
    12961 aacggttatt gcgatcactg ctgtaatgtg attttgaaat attcggctaa caatctttgg
    13021 aaagattaat tgaaaggaga cacaaatggc gattttccgt gaataccaaa aatatggccg
    13081 tgacaggagt attgaagata gaagaaggca taaaaaactg gttgaaaaaa gtataaaaga
    13141 aaacattgga agaattattt cagaagaaag tattattggt cagtccaaca ataaaaagat
    13201 aaaaattccc ataaggggtt taaaagaata ttattttagg tatggttcca atcaaaaaga
    13261 aatcttttca ggagatggga aagaaaaaaa aggagacaaa tatttaatta acaaatataa
    13321 gtttggtaaa gaagaaggaa gagccggtaa tgaggaagga gaagacatct atgaaacgga
    13381 aataaccatt gaagaagcta tacagtattt gtttgaagac ataaatcttc cttatttata
    13441 tagaaaggaa tttacagaaa ccgaatcaga aagtactggc aagaaatggg gtattcaagt
    13501 aaaaggggcg cctcccagac tctcaaaaaa gcatacagta aaagaaagaa ttaaaagaaa
    13561 aaaacaagcc cagcgaaatc aagtcataaa agaaggagat aggttcccgt ttcatgaaga
    13621 agatttaaga tattaccgct taaagcctaa gaaaaaaagg gattccaatg gggttgtcat
    13681 ttgcattatg gatacatcgg gttccatggg tcagactaag aaatatttgg caagaagttt
    13741 ttattttctt ctgtatcagt ttttaaaaat caaatacttg aatgtagaag ttgtttttat
    13801 tgcccatacc accgtagcca aagaagtaag tgaggatgag ttttttcata aagcggaatc
    13861 agggggcaca tacataagca gcggttataa gaaagcatta gaaatcattc atgaaagata
    13921 taatcctaag acctggaata tctatgcttt tcactgcagc gacggggata attggggaga
    13981 agataatcaa agagctgttg aagccgctct ggaactctgt aaggtttgta atctttttgg
    14041 atatggggaa attaagacca gtacttacac gagtacaatt atgcaaaaat acatggaaga
    14101 aatcaaagaa cccaattttg caatggtcaa aattgctggc aaggaagatg tctgggctgc
    14161 ctttgtcgag ctgcttaatg tagaaaaaaa caacagactg gaaggatgat accatgggct
    14221 attctatgga ttattcgata gaagaattaa agttttggaa tgaaaaaatt gaaaggctgg
    14281 cagaggaaaa aggcttggat ttttatcctc aggaatttga aatcataagt tatgaggata
    14341 tgcttgggta tgaatcttat ttaggcatgc cttctcatta tcctcattgg agctttggga
    14401 aaacttatga aagacttaaa acactttata aatataacct gacgggattg ccctatgaaa
    14461 tggtcattaa ttctaatccc tctattgcgt atttgatgaa agataatacc ttggttcttc
    14521 agattttaac catggcccat gtctatgccc ataatgattt ttttaaaaac aaccgtttat
    14581 ttcaggaata caccaatgca gatcttgccg ttgaaatgtt taaaaatcat gccggcagaa
    14641 tccgggaata cattaaaaac ccaagcattg gttatgaaaa agttgaaagc gttcttgatg
    14701 cggttcattc tataagatat caatgcgggc ttaagaaaag aaaaaaatat gaaaaaacaa
    14761 tagagcatca agaggaggaa gaagttgaaa aaaaagaatc cgacagctta ttacaatttt
    14821 taattgatta tggggactgt gaagaatggg aaaaagatat tatttatatt gtcatggaag
    14881 aaacgagata ttttatcccg cagattgaaa gcaaaatcat caatgaaggc tgggcttgct
    14941 ggtggcacta tcaattactg aatgatcttg atttggatta tggcagtcat attgattttt
    15001 taagaattca taataatgtc atttcccaat atccaggcag aattaaccct tattatttgg
    15061 gctttaaaat ctgggataag attgctaaaa aaacaaggga tatgaaaaag ctttttgaaa
    15121 taagagcttt agagagggac tcttccttta taagaagata cctggatttt gatacatgca
    15181 gggaaatgaa tttaatccag ttagaagagc aagaccgttt ttatgttatc actgagttgg
    15241 cagacgatga agggtggaaa agtattagaa attacctggc tgaaagtgta ggtatgggaa
    15301 ggattcctaa gattgtggtt gatgatgtta taaaaagtga caatctctta tgccttaaac
    15361 aaatttatga tggcagggaa ctggatgtaa cctatgcttt tgaaacttta aaatatattg
    15421 taaagctatg gggtggaaaa gttagtcttg atacagttat caataaaagt cccaaaagaa
    15481 tcatttgcga taaagagttt agaatttctc ttgaaaatat tgaagattaa ctcttctatg
    15541 gcaaaaagcc cttggaatag acaagggctt ttttttatta tacaatagga taggttaatt
    15601 cgatagagag aggaaagaca atgggattta aattttgttc aataacaagt ggcagcagcg
    15661 gcaattgcat ttatatagga actcctaaag tgaatatatt agtggatgca gggattagtg
    15721 ggactaaggt tcaggccggc ttagaaaaaa taggcgtaaa tcctgccagt cttcacggga
    15781 ttttaatcac ccatgaacat atcgaccata tcaaaggcgt agggattttg tccagacggt
    15841 ttgaccttcc tttattagcg acagccaaaa cctgggaagc catggaggct atggggaagg
    15901 ttctggggaa aatagctccc cacaacaagg tggtcattga aaaagataaa gattttattt
    15961 taaaagattt agtgatccat ccatatccta ttcctcatga tgcagcagac ccggttggat
    16021 atacctttgt ccatagcaat aagaaaataa gcattgctac ggatttgggg catgtcaatg
    16081 atcagataaa agataatatc agagaatcta atcttattct tttagaatcc aatcacgata
    16141 tagatatgct aataaacggc tcatatccat attatcttaa acgaaggatt ttaagtaatt
    16201 tcggtcattt gtgcaatgaa gcagcaggaa aaattctggc agaagtcttt cacgataaat
    16261 tagaagctgt tattttaggt catcttagca aagaaaataa ttatcctcag ttggcattaa
    16321 acagtgtaaa aaaacagctt cttagtgcag gcatcgaaca agatggccgg tttgagctga
    16381 ctgtagccaa aagagaggat gtatcagagt tctttagcct ttaaataggc tttttctaag
    16441 tgggatgtat aaaattcctt tctttgcttc ataatgataa tgagggtgat atgacatata
    16501 aaagaaaggg gtgcccctat ggctaaaaag aaaaaaatag acaaggctag tcaaccggct
    16561 tcatttgcca ctgaagaatt tgcatcggaa tccatcccac agcaaggttc acaaaaaagc
    16621 aggaaaaaga gcggtaaaga aaaaaaagaa atagtttagc tcgagctttt aaagcaagaa
    16681 aagaggatat tggacaaata aatccaagat cctctttttg ttctttattt cttcataaat
    16741 tatcttatta caatgtcaaa aaattggtag tagaaggaaa aatattttta tagactatgg
    16801 caaaagatta ttgtataatg atgaacgtta ctacttttaa aaagaaggtg tatcgttgag
    16861 attatccagt catgtatttg tggcggacta tgtagcaaat atactggaag aattggttgg
    16921 tgtaaaattt aatcgtcgct ttttgattct tggtgctaaa atgccagata tacagccaat
    16981 ccgcagaatg gaaatacatt cgccgcaagt tatcttacct cattttgaaa gagaatacga
    17041 tagaattgtt ttggaagaaa agaaaatcaa ccggatttca tatatattag gcctattaac
    17101 ccattatatc agtgatggct tttgttattc ccacaatgtt tataccatcg acatgaaaaa
    17161 acatatcc
//
LOCUS       DAOSUD010000045        16862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2108_length_16862_cov_22.737014, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000045 DAOSUD010000000
VERSION     DAOSUD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 16862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16862
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_16862_cov_22.737014"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1433
                     /locus_tag="PLG49_06970"
     CDS             30..1433
                     /locus_tag="PLG49_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOQ17097.1"
                     /translation="MNVQIFSFDFTTELAKSFNNGSVKNANSQKGSFEDLLNDSYENK
                     NIFKSKASTTGNANSKINVSEKDYHSHKSDTLKKVVTTDDSQINPEPLPEERQIIKNI
                     AEKTDMSEEEIVESLQQLGLTVYDLFSPTNINLFLQKLFNVTSSIDLLSIPDVNQLYK
                     DIMSIMEHLKVAFPFVDTHFMQAEETVKFNLKNDSEDFILNSVVDQQSMNNIDHSELM
                     EQVDHIIDQDLEQNARLLDKSQEQRSGEEVLTLESKKNNPQSNKKTFDNKFNNDESFL
                     NEDTFANSEEMRLENRNIIIQQNFETHLQSTENITENVTGRTTNDLMNQQNIVEQMVE
                     QIKINVGETSSEMSLQLKPDHLGKLSLKIVTERGIVTAQFMAENQAVKEIIEANFNQL
                     KDALSEQGLEVKNLEVFVKQDFQKHHSNFMKEKSSKSGKRISNILSDFVNNPEETYLN
                     PADNPYQRSESIIDYSA"
     gene            1458..2267
                     /locus_tag="PLG49_06975"
     CDS             1458..2267
                     /locus_tag="PLG49_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOQ17098.1"
                     /translation="MADVQKVNYIDPNDSRIQYSKQTTTPKNELSKDAFLNLLVTQLR
                     YQDPLNPTDDKEFLAQMAQFSALEQMQNMNKTLEVTKAFALIGKNIQATVVNRQTSDV
                     EIVEGKVDFVKIANNKAYLVVGDREVAVEDVNMVTDASLSAIDDGPSNPFELIGKVVQ
                     FTRNNPLNNKQEYVEGFVKHINMKNGIPYLVIGTDDYYLETTMDKIVGVVTKESITGK
                     KVVGKYFNPEMNDYEEVEGTVNYILMRDNQMYVIVNGKELAYEDLIKVYEG"
     gene            2284..2670
                     /locus_tag="PLG49_06980"
     CDS             2284..2670
                     /locus_tag="PLG49_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOQ17099.1"
                     /translation="MKINHNPVKITPINTLNVNKVHHQNSNAGVSEFQQLLQNQIASD
                     KNIKFSKHAAMRLDARNIAFTKEQFEKLEKGIKKAEEKGIKDSLVLMDQIALVVNIKN
                     RVVITAIDSSQAKDTVFTNIDGAVII"
     gene            2773..4116
                     /locus_tag="PLG49_06985"
     CDS             2773..4116
                     /locus_tag="PLG49_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOQ17100.1"
                     /translation="MMRSMFSGISGLRVHQTKMDVIGNNIANVNTTAFKSSRVTFNEV
                     FSQTLQGASAASIETGRGGRNPMQIGLGTNISSIDTLMTTGAAQRTDNPFDLMIEGDG
                     FFIVGDESGTYFTRAGVFRKDNDGNLTIPNGMKVMGWPAVDNGTRIQRGTVEYLKLDK
                     PENITADPEATTNLRIQGNVNIQDGISGNGIPIQLKFYDSLGNLYTANLYMTYDATAA
                     KWSINISDTLQLTDANGNVFTLENVGAEDDSITIVFDSDGNLDIKNSGGTDGTFTIPN
                     GRNSFDLTTDLSAGDPIIKSTIGPLTVDFSGLTQYNQKTNIDPLMGDKNGLGAGREPG
                     ELTGYSIGADGIITAQYSNGQQKMLGQIVIANFRNPAGLQKVGDNLFITTPNSGEFDG
                     IGSEPNLQGGVLEMSNVDLAKEFTEMITTQRGFQANSRIITTSDEMLQELVNLKR"
     gene            4190..4393
                     /locus_tag="PLG49_06990"
     CDS             4190..4393
                     /locus_tag="PLG49_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOQ17101.1"
                     /translation="MIKVTRLNGSYMVINSDLIEFVEETPDTVITMTTGKKIVVKENV
                     DEIIQRVIEYKQKILADISNKVR"
     gene            4399..5193
                     /locus_tag="PLG49_06995"
     CDS             4399..5193
                     /locus_tag="PLG49_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOQ17102.1"
                     /translation="MDISSILGLILGFVFMIISIQMNGRLGLFFDLPSIMITFGGTFA
                     SLLVSYPLNKFMNSLKTVRLIFKTQEFNTGNIIQKIIELANIARKEGLLALEEAAQDM
                     EDKFLQKGVLLIVDGTDPELVRNILETELAFIEGRHKEVQDFWGNMASEGPAWGMIGT
                     LIGLIAMLDQLNDPSTIGPSMAVALVTTFYGSVLANFFASPTANKLKVRSDEEILLKE
                     VMIEGLLSIQAGENPRIIEEKLKAFLSSTLRETMGKNNGEGPEAGE"
     gene            5196..5972
                     /locus_tag="PLG49_07000"
     CDS             5196..5972
                     /locus_tag="PLG49_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ17103.1"
                     /translation="MSKRKKVIEVNKGTATWLNTYGDMVTLLLTFFVLLFSMSTVDIA
                     KFKAFINSMEGSIGILTGTGSTIGEGSEIGNGINQLPDLEKYLSETSPEIDLKRFEEL
                     QKMYSDFKNFVTENQLQDAIDVDLGDHFVTVTFKDGILFDSGKADLKPEALTILDKIG
                     VQLKKYPNNRIRFEGHTDNRPIQTVQFPSNWELSAARAIAVAKYYINEWDFNPSQFST
                     EGFGEYVPVADNSTPEGRAKNRRVEIKILSEYAPYAGSDN"
     gene            5996..6499
                     /locus_tag="PLG49_07005"
     CDS             5996..6499
                     /locus_tag="PLG49_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOQ17104.1"
                     /translation="MEKNNKIFLYVILGIIFTALIAVIVALSFILSSFKSMANNNENK
                     NVNAEQNKAIVNSEDIIIFSIDDPITANLMQESANDDKHMLRISVGLALDKTKKDYKK
                     VNEQLSEKLPVLKHIIINVVRNKKYEEVLQPNAQELLGQEILNEINEYFQTDTITDIY
                     FGEFFVQ"
     gene            6539..7525
                     /gene="fliM"
                     /locus_tag="PLG49_07010"
     CDS             6539..7525
                     /gene="fliM"
                     /locus_tag="PLG49_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOQ17105.1"
                     /translation="MGEVLSQSEIDELLKALNTGEIDVTEIQESKQEKHIKKYDFARP
                     SKFAKEQLRTLEIIFDNYSRIISTYLSGYLRTPTQVDVINAEAVTYYEFSNSLSNPVI
                     LSIVDFSPLKGSIVIDLSPNLGYSIIDRILGGRGAMIDKIREFTEIERILLERIVTQL
                     IALLKEPWENVVDLNPKLEKIETNSQFAQIISPNEMIALVTLNIKLGEVEGMMNICIP
                     HLVLEPVLERLNTKYWFAVVEQEDKSAYRKFLEKRLEIAKIPIRAVLGKTHITVEEFI
                     HLQVGDVIKLDSYINSDLEIYAGNLLKFYGKPGIHRKQNAVQITAVERREEI"
     gene            7526..8701
                     /gene="fliY"
                     /locus_tag="PLG49_07015"
     CDS             7526..8701
                     /gene="fliY"
                     /locus_tag="PLG49_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOQ17106.1"
                     /translation="MGDMLSQAEIDALLGGTDIDEDNGFDNLDNEAITINDPLTEEEK
                     DALGEIGNISMGTAATTLFTLLNQKVIITTPNVKVMTWEQIASEYGESMVAVNVEFKE
                     GLEGINLLILKESDVKIIADLMMGGEGENTDEELTELHLSAISEAMNQMVGSASTSMS
                     SIFNKKIDIKPPHAFIPKLEKEKMIESLGFGEEGIVRVSFKMKIGELIDSEIMQILPV
                     DFAKNLASNLINSQRSSAGTKVETKTEAESAKAVEPTPSQVVIEPQQQNQTNQSYTYY
                     ESPVTQPSGFGQPNINVQPAQFQSFDQTAVIQTKENIEIIKDVPLEVTVELGRTHKLI
                     RDILEFSPGTVIELDKLAGEPVDVLVNGKFVAKGEVVVIDENFGIRITDIVNPDKRI"
     gene            8811..9173
                     /locus_tag="PLG49_07020"
     CDS             8811..9173
                     /locus_tag="PLG49_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ17107.1"
                     /translation="MGKTVLIVDDAAFMRMMIKDILVKNGYEVAGEAENGVVAVEKFK
                     ELAPDLVIMDITMPEMDGIQAVREIKKIDGNAMIIMCSAMGQQAMVIESIQAGARDFI
                     VKPFQAERVVEAVRKVIG"
     gene            9252..9662
                     /locus_tag="PLG49_07025"
     CDS             9252..9662
                     /locus_tag="PLG49_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOQ17108.1"
                     /translation="MNINWFKMIGQFFFLIVLFVVVLFAAYYVTKLIGTIQYQKYQGN
                     NIKIIETVGISPQKSLQLVKVGNKFYLLAITKDNIVFISEIDEKEINISDNIHQVPNL
                     KFDSLFKDMIKNVRNSSRKKNSNIDGEILDEKDR"
     gene            9646..10452
                     /gene="fliP"
                     /locus_tag="PLG49_07030"
     CDS             9646..10452
                     /gene="fliP"
                     /locus_tag="PLG49_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544035.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOQ17109.1"
                     /translation="MKRIDKKKVIKIITLTIMLIIFFSVISYGQEIDEQNLPIPHINI
                     DLEPAEGPQEVVSTLQILFIITIISLAPSILIMMTSFTRIIIVLYFLRSAIGTQQTPP
                     NQVLIGLALFLTLFIMSPTLADINEQAIKPYTAGQITQAEAIDNAMNPLREFMFRQVR
                     TSDLNLFMGISNAEPIENAENIEQIKEQIPSRVLIPAFIISELKTAFMIGFLLYIPFI
                     VIDMVVASTLMSMGMMMLPPAMISLPFKILLFILVDGWNLVIGQLVQTFK"
     gene            10464..10730
                     /gene="fliQ"
                     /locus_tag="PLG49_07035"
     CDS             10464..10730
                     /gene="fliQ"
                     /locus_tag="PLG49_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOQ17110.1"
                     /translation="MEQLIIDLAQEAIMTVIKVSAPMLLMGLAVGLLVSIFQAVTSIQ
                     EATLAFIPKILAVFISILIFGPWMLSTMMEFINNIYKNLNLYIR"
     gene            10744..11529
                     /gene="fliR"
                     /locus_tag="PLG49_07040"
     CDS             10744..11529
                     /gene="fliR"
                     /locus_tag="PLG49_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOQ17111.1"
                     /translation="MDFLMSNVFLKVDILLLVFVRFLGLFVTSPVFGGRNVPNYSKIG
                     FSLIISTLLLSVKQNITVSYDNHVIGYALLIMKELIIGMLLGFVVYLILSVFYLAGQL
                     IDYQIGFTMVSVMDPLSQIQVPVTGNFYYLLVLILFLIRNGHHKLIQALFYSYDVLPI
                     GEAVFTNQLTGSYIHIVANSFVIAFKIASPFITVVLILDVALAVLARTVPQLNMFVVG
                     LPLRLLLGLACLFIVASLLSSIFSYVSNELFSELFKVIKGLIP"
     gene            11526..12650
                     /gene="flhB"
                     /locus_tag="PLG49_07045"
     CDS             11526..12650
                     /gene="flhB"
                     /locus_tag="PLG49_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOQ17112.1"
                     /translation="MIINHKNSNDCPILYYDLQFFADKDSKTEKPTPRKREKAREEGQ
                     VAKSMEINTAFLLIFMFAGLKIFASFMYKRITSVFKNTYMLFPQLDDLFNVSSMLKFA
                     SDIFTQTLIIVAPLFAISMVVGFSVNFAQVGWHPTLKPLKPNFDRLNPIKGFSRLFSK
                     QALIELLKSIGKIAIIAVIIYNCIVDEMENLLLLLDMELMPFIIYIGSLLINIGLKVG
                     LFFIFVAAADYFFQRYEFEQNLKMTKEEVKEEYKMTEGDPQVKSKIRQKMREVSLRRM
                     IKDIPKADVIITNPTHYAVALQYDSSVHSAPVVIAKGVDYLAMRIKNIAKENNIEIVE
                     NKVLARTLYQTVEIGEEIPPELYQAVAEILAFVYSLRHSY"
     gene            12686..14737
                     /gene="flhA"
                     /locus_tag="PLG49_07050"
     CDS             12686..14737
                     /gene="flhA"
                     /locus_tag="PLG49_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOQ17113.1"
                     /translation="MNFKFGDIFLGVFVIAIIAMIIVPLPLFLLDILLILNISLSLMI
                     LLTALFSKEPLELSTFPTILLITTLFRLGLNISTTRLILGQGDAGAVVRTFGSFVAGG
                     NIVLGVIVFIIIVIIQFLVITKGAERVSEVSARFILDAMPGKQMAIDADLNTGLIDEA
                     EAKLRRKKIQDEASFYGAMDGASKFVKNDAIAGIIITFINIIGGIILGTTGIVTKKPM
                     PIMEALDVYTVLTIGDGLVSQIPSLLISTATGILVTKTASESDMSSDLIKQLGSTPRV
                     LQITGVTIIILGLVTPLPDYITLPVGILLLLGANTIDKNLKLQSISEEISSEDIEAEE
                     VRRPENVVSLLKVDPIELEFGYGIIPLADINQGGDLLDRVVMIRRQIALELGAIVPII
                     RLRDNIQLSPNQYVIKIKGVEVSKGEILFDHYMAMNPGYVEEEIDGIETVEPAFGLPA
                     LWISESQREKAEILGYTVVDPPSIIATHLTEIIKRHIHELLSRQDVQTLVDNVKETHP
                     ALVEELVPKILTISEIQKVLANLLKESVSIRDLVTIFETLADYGTITRDTDLLTEYVR
                     QNLSRAISKKFLNQPSNKVITLDPDLEQKIMDSVQQSEQGSYLALDPESTQKIFERLN
                     QEIRRFNSIGQQPILLTSPIVRIYFRRLTEQVAPDLIILSYNEIDPSIEIQSIGMVSI
                     S"
     gene            14767..15906
                     /gene="flhF"
                     /locus_tag="PLG49_07055"
     CDS             14767..15906
                     /gene="flhF"
                     /locus_tag="PLG49_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOQ17114.1"
                     /translation="MKIKKYEAKTEQEAIEKVKEDLGKDALILNIKKITPKGIFKLFK
                     KPYVEVLAALDEVNKKDSKKVQADDIIKEQKEIIKNLENKLDSMEELLTKVMEKVSTT
                     SQVKDSKEDNQRYHNPALQIIYDNLTNNEVLPELADYILKDLDKDKELNPEDVNEMIT
                     VIYNRILDLIGEPAPIKADGSQPKVVFFIGPTGVGKTTTIAKITAHFCLTERKKVGLV
                     TADTYRIAAIEQLKTYAEILNIPVEVIYNPDELPETLNKMKDYDLILIDTAGRSHKNA
                     EQFKELDYLLSTVNEKQIYLVLSATTKHKDMLSILKQYTSISDYNIIFTKIDETTSLG
                     TILNVRYLTDKPLSYVSFGQNVPDDIEIISPKKLTKALLGSIDEL"
     gene            15903..16796
                     /locus_tag="PLG49_07060"
     CDS             15903..16796
                     /locus_tag="PLG49_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOQ17115.1"
                     /translation="MMDQAEKLRKIVNEQNRQLENSVSKTRKSRVITITSGKGGVGKS
                     NFTANLAIQLQRTDRKVVIIDADLGLANIEVIFGIVPQFNLTDVIYGRKSIAEIMTPG
                     PLGINFISGGSGIQDLIHLNSQQLSNFISNLSLLDEYADIILIDTGAGLTDSVINFSK
                     AADEIILVTTPEPTSVTDAYSLIKVLCKNNLIPNIKLLVNRVEDDEEGKEIFNKLYRV
                     SKKFLNIEINELGYLPYDKNLIKSVKLQEPVCISFPNSEISRAFRKISYKITEDEPQE
                     EMNSGFTSFVKRLMHVFERNN"
BASE COUNT         5962 a         2402 c         3172 g         5326 t
ORIGIN      
        1 acaataatga aatgaaagga gggtagggga tgaatgtaca aattttttca tttgatttta
       61 caaccgagtt agcaaaatct tttaataatg gttcagtgaa aaatgcaaat tctcaaaaag
      121 gaagctttga agatttgtta aacgacagct atgaaaacaa gaatattttc aaatcaaaag
      181 caagcacgac tggtaatgct aacagtaaga tcaatgtatc agaaaaagat tatcacagtc
      241 ataaaagcga tacattgaaa aaggttgtta caacagatga ttcacagatt aatcctgagc
      301 cattgccgga agaaaggcaa attatcaaaa acattgctga aaaaacggat atgtcagaag
      361 aagagattgt ggagagtttg caacagttag gtctgactgt atacgatttg ttttctccga
      421 ccaatattaa tcttttcctt cagaagttat tcaatgtcac aagttctatt gatttattat
      481 ccattcctga tgttaatcaa ttgtacaaag acataatgag tataatggaa catttaaaag
      541 ttgcttttcc tttcgttgat actcatttca tgcaagctga agaaacagtg aaattcaatc
      601 taaaaaatga cagtgaagat ttcattttaa attcagttgt tgatcaacag tctatgaata
      661 acattgacca ttcagagctt atggaacaag ttgaccatat cattgaccag gatttagagc
      721 aaaatgccag acttctggat aaaagccagg aacagcgttc tggagaagag gttttaacac
      781 ttgaatctaa aaagaataac ccacaaagta ataaaaagac ttttgacaat aagtttaata
      841 atgatgagag ttttttaaat gaagatactt ttgctaattc agaagagatg cgattagaaa
      901 atagaaacat aattattcag caaaattttg aaacgcatct tcaaagtaca gaaaacatta
      961 ctgaaaatgt aacaggaaga acaacgaatg atttaatgaa tcagcaaaat atcgtagaac
     1021 aaatggtaga acaaatcaaa atcaatgttg gtgagacaag ttcagaaatg agtttacagc
     1081 ttaaaccaga tcatttaggt aaattgagct taaaaattgt tacagaaaga ggtattgtta
     1141 ctgctcaatt tatggcagaa aaccaggcag ttaaagaaat tattgaagcc aatttcaatc
     1201 agttaaaaga tgcactcagt gaacaaggcc tggaagttaa aaatcttgaa gtatttgtaa
     1261 aacaagattt tcaaaaacat cattccaatt ttatgaaaga aaagtcatca aaatctggaa
     1321 agagaattag caacattctt tcggattttg taaataatcc cgaagaaact tatttaaacc
     1381 cggcagataa tccttaccaa cgttcagaaa gtatcattga ttattctgca taaattctta
     1441 agcttggagg tgaaagcatg gcagatgttc aaaaagtcaa ttatattgat cctaatgatt
     1501 caaggataca gtattccaag caaacgacca ctccaaaaaa tgagttaagc aaagatgcat
     1561 tcttgaatct tctggtaacc caattaagat accaggatcc tcttaatccg acggatgaca
     1621 aagaattttt ggcccaaatg gctcaattta gcgctttaga gcaaatgcaa aatatgaata
     1681 aaactttaga agtaacaaaa gcttttgctt taatcggaaa aaatatacaa gcaaccgttg
     1741 tcaatcgtca aacatcagac gttgaaattg tggaagggaa agttgacttt gttaaaattg
     1801 caaataataa ggcttattta gtggttggag acagagaggt tgctgttgaa gatgttaata
     1861 tggtaacaga cgcaagctta tcagcgattg atgatgggcc aagtaatcct tttgaactga
     1921 ttggtaaagt tgttcaattt acaagaaaca atccactaaa taataagcag gaatatgttg
     1981 aaggttttgt aaaacatatt aatatgaaaa atggtattcc ttatctggta attggcacag
     2041 acgattatta tcttgaaacc actatggata aaattgtagg tgtcgtgacc aaagaatcca
     2101 ttacgggaaa aaaagtagtt ggtaaatact ttaatccaga gatgaatgac tatgaagaag
     2161 ttgaaggtac agtcaattat attttgatga gggataatca aatgtatgtt attgtaaatg
     2221 gtaaagaact ggcatacgaa gaccttatca aagtatatga gggctaataa aggtggtgag
     2281 tttatgaaaa taaaccataa ccctgttaaa attaccccta taaatacttt aaatgtcaat
     2341 aaagttcatc atcaaaatag caatgctggg gttagtgaat tccagcaact actacaaaat
     2401 caaattgcgt ctgacaaaaa tataaagttt tccaaacatg cggctatgcg tttagatgcg
     2461 cgtaacatag cttttacgaa agagcaattt gaaaaactgg aaaaaggaat taaaaaagca
     2521 gaggaaaaag gtataaaaga ttctttggtt ctaatggatc aaattgcttt agttgttaat
     2581 attaaaaatc gggtggttat cactgctata gattcaagtc aggccaaaga tactgttttt
     2641 accaatattg atggtgctgt aatcatataa gctggacctc ttgtaggaga gcttttcatc
     2701 ttggattgac tgaaaagatg agtgtattac tgcaccagga ttagcttatt tatttaagga
     2761 ggtcgtttta ctatgatgag atctatgttt tccggtattt ccggtttaag agtacatcaa
     2821 acaaaaatgg atgttatagg taataatatt gccaacgtaa ataccacagc ctttaaatcc
     2881 agccgggtta cttttaatga ggtatttagc caaactcttc aaggggccag tgcagcaagt
     2941 attgaaacgg gcagaggtgg aagaaatccg atgcaaattg gattaggaac caatatttct
     3001 tccattgata ctttaatgac aaccggagct gctcaaagaa cggacaatcc ttttgattta
     3061 atgatagaag gagacgggtt ctttattgtt ggtgatgaaa gtggaactta ctttaccaga
     3121 gcaggtgtat ttagaaaaga caacgatggt aatttaacga ttcctaatgg aatgaaggtt
     3181 atgggatggc ctgcggttga taatggtacc aggattcaaa gaggcacagt tgaatatttg
     3241 aaattagata aacctgaaaa cataacagcc gaccctgagg ctacaaccaa tcttcgaata
     3301 caaggtaacg taaacattca ggatggtatt tccggaaatg gaattccaat tcaattaaag
     3361 ttttatgaca gtttaggcaa cttatataca gcaaatcttt atatgacgta tgacgcaacg
     3421 gctgctaaat ggagtattaa tatatctgat accttgcaac taacagacgc gaatggtaat
     3481 gtctttacgt tagaaaatgt aggggctgaa gacgattcta ttactattgt atttgattcc
     3541 gacggaaatc tggatattaa aaactctggt ggaaccgatg gaacttttac tattcctaat
     3601 ggtagaaatt cttttgattt aactactgat ttaagtgccg gagatcctat tattaaatcc
     3661 acaataggac ctttaacagt tgatttttct ggattgaccc aatacaatca aaaaaccaat
     3721 atcgatcctt taatgggtga caaaaatgga cttggagctg gtcgggaacc tggtgaatta
     3781 acgggttata gcattggtgc agatggtatc attactgccc aatacagcaa tggccaacaa
     3841 aaaatgttag gtcaaatcgt tattgcgaat tttagaaatc cagctggtct tcaaaaggtt
     3901 ggagataact tatttattac cacgccaaat tctggtgaat ttgacggaat cggaagtgaa
     3961 cctaatctcc aaggtggtgt gttagaaatg tctaatgtag acctcgctaa agaatttaca
     4021 gaaatgatta caactcaaag aggttttcag gcaaattcaa gaatcattac cacatcagac
     4081 gaaatgcttc aggagttagt taatttaaaa cgttaattaa ctttctcgca ctttaagaca
     4141 tttttgtctt aaagtgcgag gtaatttgat gtccaaaaga gggataaaga tgattaaagt
     4201 aacaaggtta aatggtagtt atatggtgat caattcggat ttaattgaat ttgtggaaga
     4261 aacacccgat acggtcataa ccatgactac aggtaaaaaa atagttgtaa aggaaaatgt
     4321 agatgagatt atccaacgtg tcatcgaata taagcaaaaa atactggccg atattagcaa
     4381 taaagtgagg tgattgcatt ggacatttct agcattttag gactgatatt aggatttgtt
     4441 ttcatgatta tttctattca aatgaatggt cggttaggct tattttttga tttaccttct
     4501 attatgatta cttttggagg tacttttgcc tcactgttag tttcctatcc gttaaataaa
     4561 tttatgaatt ccttaaaaac agttcgttta atttttaaaa ctcaagaatt caatacaggt
     4621 aacattattc aaaaaattat tgaattagct aatattgcaa gaaaagaagg tttgctggct
     4681 cttgaagaag ctgcgcaaga tatggaagac aagtttttac aaaaaggcgt tttattaatt
     4741 gttgacggaa cagatcctga actggttaga aatatattag aaactgaatt ggccttcata
     4801 gaaggacgtc acaaagaagt gcaagatttc tgggggaaca tggcaagcga aggacctgcc
     4861 tggggtatga taggtacctt aattggactt attgctatgt tggaccagtt aaatgaccct
     4921 tctaccattg gacccagtat ggctgttgcc ttagttacaa ctttctatgg atcagtttta
     4981 gcaaactttt ttgcatctcc tacagccaac aaattgaaag tcagaagtga tgaagaaatc
     5041 cttttaaaag aagtgatgat agagggatta ttgtctattc aggcgggaga aaacccaaga
     5101 attattgaag agaaattaaa ggcattctta tcatctactt tacgtgaaac catgggcaag
     5161 aacaatggtg aaggtccgga ggcaggtgaa taaaagtgtc taaaagaaaa aaagtaatag
     5221 aagtaaacaa gggcactgca acatggctga atacttatgg tgatatggta actttgttgc
     5281 ttacgttttt tgttcttctt ttctcgatgt ccacagtaga tatcgcaaaa tttaaagcct
     5341 ttattaattc tatggaaggt tcaattggga ttctgacagg aactggttct actatagggg
     5401 aaggcagtga aattggtaat ggtattaatc agttacctga tttagaaaaa tacctgtcag
     5461 aaacaagtcc tgaaattgat ttaaaaaggt ttgaagaact tcaaaaaatg tactctgatt
     5521 tcaagaattt tgttactgaa aatcaattac aagatgccat tgatgttgat ttaggagatc
     5581 attttgttac tgtgaccttt aaagatggga tattgtttga cagtggtaaa gcagatttaa
     5641 aaccagaagc ccttaccata ttggataaaa taggtgttca actaaagaaa tatccaaata
     5701 acagaattcg atttgaaggg cacacagata acaggccgat acagactgta caattcccca
     5761 gcaattggga attatctgct gcccgggcaa tagccgtagc aaaatattat attaatgaat
     5821 gggattttaa tcctagtcaa ttttctactg aaggttttgg ggaatacgtc cctgttgctg
     5881 ataattctac tcctgaggga agagcaaaaa acaggagggt tgaaattaag attcttagtg
     5941 aatatgcacc atacgccgga agcgataatt gatggattcg ttaaggggga ttctaatgga
     6001 gaaaaacaat aaaatctttc tttatgtcat tttaggaatt atttttactg ctctaatagc
     6061 agtaattgta gctctttctt ttatactaag ttcatttaaa tctatggcaa acaataatga
     6121 aaataaaaat gtaaatgcag agcaaaataa agcaattgtt aattcagaag atatcatcat
     6181 tttttcaatt gatgatccca taactgctaa tcttatgcaa gaaagtgcaa atgatgataa
     6241 acatatgctt cgtatttctg taggattggc gttagataaa actaagaagg attataaaaa
     6301 agttaatgaa caactgtctg aaaagttgcc tgtccttaaa catattatta tcaatgttgt
     6361 aaggaataaa aaatatgagg aagttttaca gccaaatgcc caggaattat taggtcagga
     6421 aatattaaat gaaattaatg agtattttca aaccgataca atcacggata tctattttgg
     6481 ggaattcttt gttcaatagg gatatgactt acttatatag tggccaggag gtgattaagt
     6541 gggcgaagtt ttatcacaat ctgaaataga tgaactgcta aaagcattaa atactggaga
     6601 aattgatgta acagaaattc aggaatcaaa gcaagaaaag catattaaaa aatatgattt
     6661 tgccagacca tcaaagtttg caaaagaaca gttaagaaca ttagagatta tttttgataa
     6721 ttattcaaga ataatatcta cttatttgtc tggctattta agaacaccca cacaagtcga
     6781 tgtaattaac gcagaggccg ttacttacta tgaatttagt aattccttat ctaatcctgt
     6841 aattttgtca attgttgatt tttccccttt aaaaggttca attgtaatag acctttctcc
     6901 aaatttaggc tattcaatca ttgacagaat tttaggcgga agaggtgcaa tgattgataa
     6961 aataagggaa tttactgaaa tagaacgaat actattagaa aggattgtta cacaacttat
     7021 tgctttatta aaagagccat gggaaaacgt agttgattta aatccgaaat tagaaaagat
     7081 tgaaaccaat tcccagttcg ctcaaatcat ttctcctaat gaaatgattg cattagttac
     7141 tttaaatatt aaacttgggg aagtagaagg catgatgaat atctgtattc cacatttagt
     7201 tcttgaacct gtcttagaac ggttaaatac taagtattgg tttgcagtgg tggagcaaga
     7261 agataaatct gcctacagaa aattcttaga aaagagactt gaaattgcaa aaatccccat
     7321 aagagctgtt ttaggaaaaa cacatatcac cgttgaagaa tttattcatc ttcaagttgg
     7381 tgatgtgata aagctggact catatatcaa ttcagattta gaaatttatg caggtaatct
     7441 cttaaagttt tatggtaaac ctggaattca tagaaagcaa aacgcagtac agataactgc
     7501 tgttgagcga agggaggaaa tttaaatggg agatatgctt tcgcaagctg aaattgatgc
     7561 tttacttggc ggaacggata ttgacgaaga caatggtttt gataatcttg ataatgaggc
     7621 aataacaata aatgatcccc ttacggagga agaaaaagac gcattaggtg aaatcggtaa
     7681 tatcagtatg ggtactgctg caacgacatt gtttacttta cttaatcaaa aagtgattat
     7741 taccactccc aatgttaagg tgatgacttg ggaacaaatt gccagtgaat acggagaatc
     7801 aatggttgca gtgaacgttg aatttaaaga aggattagaa ggtattaacc tgcttatatt
     7861 aaaagaaagt gatgtcaaga ttattgctga tttaatgatg ggtggggaag gcgaaaatac
     7921 ggacgaagaa ttaaccgaac ttcatttaag tgccattagt gaggccatga atcaaatggt
     7981 tggctctgct tccacctcca tgtcatccat atttaataaa aaaatagata ttaaaccacc
     8041 tcatgcattt atacctaaat tagaaaaaga aaaaatgatt gaaagtttgg gcttcggtga
     8101 agaaggcatt gtaagggttt cctttaaaat gaagattggt gaattaatag acagtgaaat
     8161 tatgcagatt ttgcctgtag attttgcaaa aaatcttgct tcaaatttaa ttaattctca
     8221 aagaagttct gctggtacta aagtggaaac aaaaacagaa gctgaatcag caaaagctgt
     8281 tgaacctaca ccttcacaag tagtgattga gccacaacag caaaatcaaa ccaatcaatc
     8341 ttatacttat tacgaaagtc ctgttacaca gccttctgga tttggtcagc caaatattaa
     8401 tgttcaacct gcccaatttc aatcttttga tcaaactgca gtgattcaga caaaagaaaa
     8461 tattgaaatt attaaggatg ttcctttgga agttactgtt gaattaggaa ggacccataa
     8521 attaatcaga gatatattag aatttagtcc tggtactgtc attgaattag ataagttagc
     8581 aggagaacct gttgatgttt tagttaatgg caaatttgtt gctaaaggtg aagtcgttgt
     8641 tattgatgaa aatttcggta taagaattac agatatcgtt aatcctgata aaaggatata
     8701 ataatcgata atttgacgtt tttcatggaa ttttttaatt aaatatatta taatacctaa
     8761 taaatgctat ggctgatttc tttaatattc attcaaagga gagggtattt atgggaaaga
     8821 ccgtattaat cgttgatgac gcagcattta tgagaatgat gataaaagat attttagtta
     8881 agaatggtta tgaagtagct ggtgaagcag aaaatggtgt tgtagctgtc gaaaaattta
     8941 aagaacttgc accggatttg gtgataatgg atattacaat gcccgaaatg gatggtattc
     9001 aggctgtcag ggaaattaaa aaaattgatg gtaatgccat gatcattatg tgttcagcta
     9061 tgggacaaca ggcgatggtt attgaatcta tccaggcagg cgcaagagat tttatcgtaa
     9121 aaccattcca ggctgaacgt gtggttgagg ccgtaagaaa ggttatagga tagaaaatga
     9181 atacgatatg gaatctatat ttgccttttc tattacttca gggatcagct tcattcgata
     9241 aaggaggaca agtgaatata aactggttta agatgatagg ccagtttttt ttcctgattg
     9301 tattatttgt cgtagtatta tttgctgctt attatgttac gaaattaatt ggtacaattc
     9361 agtatcaaaa atatcaggga aataatatta aaattataga aacggtagga atttctccac
     9421 agaaaagcct gcaattggta aaggtaggaa ataagtttta tttattagcc attaccaaag
     9481 acaatatcgt atttatatcg gaaattgatg aaaaagaaat caatatttct gataatattc
     9541 atcaagtacc gaatttgaaa tttgattcat tatttaaaga catgataaaa aatgtgagaa
     9601 attcttctcg aaaaaagaat agtaacattg atggagagat tctggatgaa aaggatagat
     9661 aaaaagaaag tcataaaaat catcactttg accataatgt taataatatt tttttccgtt
     9721 attagctacg gtcaggaaat agatgagcaa aatttgccta ttcctcatat caatattgat
     9781 ttagaaccag cagaaggacc tcaggaagtt gtcagtacgc tgcaaattct ttttattata
     9841 acgattatct ctttggctcc atccatatta attatgatga cttcttttac cagaattatt
     9901 attgtcctgt actttctgcg ttctgcaatt ggaactcagc agacaccacc taatcaggtt
     9961 ctgattggac ttgctttatt tttaacttta tttattatga gtcctactct ggcagatatc
    10021 aatgaacagg caataaaacc ctatacagct ggtcagataa ctcaggcaga agccatagat
    10081 aatgcaatga atcctttgcg agaatttatg ttcagacaag tcagaacgag tgatttaaac
    10141 ttatttatgg gtatttctaa tgcagaaccc attgaaaatg cagaaaatat tgaacaaata
    10201 aaagagcaaa ttccatcaag agttttaatt cctgcattta ttattagtga attaaaaacg
    10261 gcatttatga taggattttt attatatatt ccttttattg ttattgatat ggttgtcgct
    10321 tcaaccctaa tgtcaatggg aatgatgatg cttcctccag caatgatttc tctgccattc
    10381 aagattttac tttttattct ggtagatggg tggaatttgg tgataggtca actggttcag
    10441 acctttaaat aaggaggaaa aggatggaac aattaattat tgatttagca caggaagcaa
    10501 taatgactgt cataaaagta tctgctccta tgctattaat gggtttagca gttggtttac
    10561 tggttagcat ttttcaggca gttacttcga ttcaggaagc tactcttgcc tttattccta
    10621 aaatattagc tgtttttatt tcaattttaa tttttggacc ttggatgctg tcaaccatga
    10681 tggaatttat taataatatt tataaaaatt tgaatttata tattcgttaa aggaaaatgc
    10741 agaatggact ttttaatgag taatgtattt ttgaaagttg atattttgct attagtgttt
    10801 gtaagatttt taggtttatt tgtaacgtca ccggtttttg gcggaaggaa tgttcctaat
    10861 tattcaaaaa ttggtttttc attaattatc agtactcttt tgctttccgt aaaacaaaat
    10921 attacagtta gttatgataa tcatgtcata ggctatgcct tactcattat gaaagagctc
    10981 attattggaa tgctgctggg ttttgttgtt tatttaatcc ttagcgtctt ttatttagca
    11041 ggacaactca ttgattatca aattggattt actatggtca gtgttatgga tcctctaagt
    11101 cagattcaag ttcctgttac aggaaatttt tattatctgc ttgttttaat cctctttctt
    11161 attagaaatg gtcatcataa acttattcag gctttgtttt atagttacga tgttcttccg
    11221 ataggagaag ccgtttttac gaatcaattg acaggaagtt atattcatat tgtggcaaat
    11281 agttttgtta tagcttttaa aattgcaagt ccatttataa cggtagtact tattcttgat
    11341 gttgcattag ctgttttagc acggacagtt cctcagttga atatgtttgt tgttggttta
    11401 cctttaaggc tattattagg tttggcatgt ttatttattg tagcatcctt attatcttca
    11461 atattcagtt atgtttccaa cgagttattc agcgagttgt ttaaagttat taaaggattg
    11521 atcccatgat aataaatcat aaaaattcaa atgattgtcc aatactctat tatgatttac
    11581 agttttttgc ggataaagac agtaaaacag aaaaaccaac tccaagaaaa agagaaaagg
    11641 cccgggaaga aggacaagtt gcaaaaagca tggaaatcaa tacagctttt ttgctaattt
    11701 ttatgttcgc tggcctgaaa atttttgctt cttttatgta taaaagaatc acttccgttt
    11761 ttaaaaatac ctatatgctt tttcctcaat tagatgatct ttttaatgtt tcatctatgt
    11821 taaaatttgc ttctgatatt tttactcaaa cgttaattat tgttgcacct ttgtttgcca
    11881 tttcaatggt agtaggattt agtgtaaatt ttgcccaggt tggctggcac ccaactttga
    11941 agcctttaaa gccgaatttt gacagactaa atcctataaa aggtttttcc agattatttt
    12001 ctaaacaagc actgatagaa cttttaaaat ctatcggtaa aatagctatt attgctgtca
    12061 ttatatataa ttgtattgtt gatgaaatgg aaaacctttt gcttttattg gatatggaat
    12121 taatgccatt tatcatatat attggttctt tgttgattaa tattggttta aaagtcggtt
    12181 tgtttttcat atttgttgca gcggcggatt attttttcca aagatatgaa tttgaacaaa
    12241 atttaaaaat gactaaagaa gaagttaaag aagaatataa gatgacagaa ggagaccccc
    12301 aggtaaaaag caaaataaga caaaagatgc gagaagtctc tttaagaagg atgataaaag
    12361 atatacctaa agcagatgtc atcataacaa acccgactca ttatgctgtt gcgctgcaat
    12421 atgattccag tgttcattct gctcctgtag tcatagctaa aggagtagat tatctggcta
    12481 tgagaattaa gaacattgct aaagaaaata atatagaaat tgttgaaaat aaagtgcttg
    12541 caagaacact ttaccaaact gtcgaaatag gagaagaaat tcctcctgaa ttgtatcagg
    12601 ctgtggcaga aattttggcc tttgtataca gcttaagaca cagctattga tattttgcaa
    12661 tttaggataa caagggggat tttatgtgaa tttcaaattt ggcgatatat ttttaggggt
    12721 atttgttatt gccattattg caatgattat tgttccattg cctctgtttt tgttagatat
    12781 attattaatt ttaaatattt ctctttccct gatgatttta ttaactgctt tattctccaa
    12841 agaacctttg gaattatcaa ccttcccaac gatattgctg attactactt tgttcagatt
    12901 aggattaaat atctctacaa cccgcttaat tttaggtcag ggagatgcag gtgccgttgt
    12961 aagaactttt ggaagtttcg ttgccggtgg caatattgtt ctaggtgtaa ttgtatttat
    13021 cattatagta attatacaat ttttagttat tacaaagggt gctgaaagag tatccgaagt
    13081 atctgcccgc ttcattttag atgctatgcc aggtaaacag atggctatag atgcggattt
    13141 aaatactggt ttgattgatg aagctgaagc aaaattaaga agaaaaaaga ttcaggatga
    13201 agcaagtttt tacggagcta tggatggagc aagtaagttt gtaaaaaatg acgccatagc
    13261 tggaattata atcactttta ttaatattat aggtggtatt attcttggaa ctacaggaat
    13321 tgtaaccaaa aagcccatgc ctattatgga agctttagat gtttatactg ttttaacaat
    13381 aggggatggt ttggtaagcc aaataccttc tttattaatt tctacagcaa caggtatttt
    13441 ggtaacaaag acagcttcag aaagcgatat gagcagtgat ttaataaaac agttgggcag
    13501 tacccccaga gtcttacaaa ttacaggagt aaccattatt attttaggac tggttacacc
    13561 actgccggac tacattactt tgccagtagg tattttatta ctccttggtg ccaataccat
    13621 tgataaaaat cttaaactac agtctattag tgaagaaatt tcttctgaag atatagaggc
    13681 tgaagaagtc agaagaccag aaaatgttgt ttcacttctt aaagtggatc ctatagaatt
    13741 ggaatttggt tacggcatca ttccgcttgc ggatataaat caaggaggag acttacttga
    13801 cagagttgtt atgattagga gacaaattgc ccttgaatta ggggctattg tacccattat
    13861 cagacttcgt gataacattc agttaagccc caatcaatat gttattaaaa ttaaaggggt
    13921 agaagtatcg aaaggcgaaa ttctgtttga tcattatatg gccatgaatc ctggttatgt
    13981 agaagaagaa attgatggta tcgaaactgt agaacctgcc tttggtcttc ctgcattatg
    14041 gatttctgaa agccaaagag aaaaagcaga aattcttggt tatacggtcg tagatcctcc
    14101 atctattatt gctacccatt taacagaaat cataaaacga cacattcatg aattgctgag
    14161 ccggcaggat gtacaaactc tagtggataa tgttaaagaa acacatcctg ctctggtgga
    14221 agaattagtt cctaaaatat taaccattag cgaaatacaa aaagtattag ccaatttgtt
    14281 aaaagaatct gtatccatac gagatttagt gacaatattt gaaacattag cagactatgg
    14341 aacaattaca agagatacgg atttactaac ggaatatgtc agacaaaatt tatcaagagc
    14401 aatttctaaa aaatttctta atcagccaag taataaagtg attacccttg atccagattt
    14461 agaacaaaaa attatggatt ctgttcagca gtcagaacaa ggatcatacc ttgcattaga
    14521 tcctgaaagc acgcaaaaaa tatttgaaag attaaatcaa gagatccgtc gctttaattc
    14581 cataggccag caacctattc ttctgacgtc tccaatcgtc agaatttact tcagaagact
    14641 tacagaacag gttgctccgg atttaatcat cttatcttat aatgaaattg atcctagtat
    14701 tgaaatacag tcaataggaa tggtaagcat tagttagata ataaaaatga aggtatggtg
    14761 tttgaaatga agattaaaaa gtatgaagca aagactgagc aggaagcaat tgaaaaagtt
    14821 aaagaagatt taggaaaaga tgccctcatt ttaaatataa aaaagattac tccaaaaggt
    14881 atctttaaat tatttaaaaa gccatatgtt gaagttttag ccgcgttgga cgaagttaat
    14941 aaaaaggatt ctaaaaaagt ccaggctgat gatattatta aggagcaaaa agaaattatt
    15001 aaaaacttag agaataaact tgattctatg gaagaattac ttacaaaagt tatggaaaaa
    15061 gttagtacaa ccagtcaggt aaaagattct aaagaagaca atcaaagata tcataatcca
    15121 gctttacaga ttatttatga taatttgact aataatgaag tattgcctga attagccgat
    15181 tacattttaa aagatttaga taaagataaa gagctgaatc cggaagatgt taatgaaatg
    15241 ataacggtca tttataatcg gatactggat ttaattggcg aacctgctcc aataaaagca
    15301 gatggcagcc agcctaaagt cgtatttttt attgggccaa caggagttgg aaaaacaacg
    15361 accattgcaa aaatcacagc gcatttttgc ctgacagaaa gaaaaaaagt agggctggtt
    15421 acagcggaca cctatcgcat tgccgctatt gagcaattaa aaacctatgc agaaattctt
    15481 aatattcctg ttgaagtgat ttataatccc gatgaattac cagaaacact taacaaaatg
    15541 aaagattatg atttaatatt aattgatact gcgggaagat ctcataaaaa tgcagagcaa
    15601 tttaaagaat tagattattt actgtctact gtaaatgaaa aacaaatcta tcttgtatta
    15661 agcgcaacta cgaaacataa agatatgctt agtattttaa aacaatacac ctccatatct
    15721 gattataata tcatttttac aaaaatagat gaaacaactt ctttaggaac catattaaat
    15781 gtaagatatt taacagacaa gccattatca tatgtttctt ttgggcaaaa tgttcctgat
    15841 gatattgaaa ttattagtcc taaaaaattg acaaaagcat tattggggag tattgatgaa
    15901 ctatgatgga tcaagctgaa aaactaagaa agattgttaa tgaacaaaac aggcaattag
    15961 aaaatagtgt ctctaaaaca agaaaatctc gggtaataac gattaccagt ggaaaaggtg
    16021 gcgttggtaa aagtaatttt actgctaatt tggcgataca attacaaaga accgatagaa
    16081 aagttgttat cattgatgcg gatttaggtt tagccaatat tgaagtaata tttggtatag
    16141 ttccacaatt taatcttact gatgttattt atggcagaaa aagcatagcc gaaattatga
    16201 ctcccggtcc cttaggaatt aattttattt caggaggttc aggcatacag gatcttattc
    16261 atttaaacag tcaacagtta tctaatttta taagtaactt aagcctgtta gatgaatatg
    16321 cagacattat tttaatcgac acgggggcag gattaactga ctctgttatc aatttttcta
    16381 aagctgctga tgaaattatt ctggtaacta ctcctgaacc aacttccgtt acagatgcct
    16441 attctctcat taaagtgttg tgtaaaaaca atttaatacc caatattaaa cttttagtta
    16501 accgagtgga agatgacgaa gaaggtaagg aaatatttaa taagttatac agagtatcaa
    16561 aaaaatttct taatattgaa attaatgaat taggttatct gccttatgac aaaaacttaa
    16621 ttaaatccgt taaattacaa gaacctgttt gtattagctt tccaaatagt gaaattagca
    16681 gggcttttag gaaaatttct tataaaataa cagaagatga gccgcaagaa gaaatgaatt
    16741 ccggatttac ctcgtttgtt aaacgcttaa tgcatgtgtt tgaaagaaat aattaataag
    16801 caattatttc aacaaaggag gcacttacgt gaaaaatatt ttttcaccgg gagttaaagt
    16861 tg
//
LOCUS       DAOSUD010000046        16392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2181_length_16392_cov_26.456449, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000046 DAOSUD010000000
VERSION     DAOSUD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_16392_cov_26.456449"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLG49_07065"
     CDS             <1..269
                     /locus_tag="PLG49_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ17116.1"
                     /translation="DSLDWKEYGVDPMIKQVLNHKHLGNGSIILFHNNAKYTKDALDA
                     IITGLKEQGYEMVPISQLILKDNYYIDHEGRQRPIKDNKQARNK"
     gene            complement(313..498)
                     /locus_tag="PLG49_07070"
     CDS             complement(313..498)
                     /locus_tag="PLG49_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HOQ17117.1"
                     /translation="MGQENKNTTEEIEILKAALETLLRQRNYNFLDPLVQYLSRKIDS
                     LINKLIEEQSNCTEPKD"
     gene            786..1151
                     /locus_tag="PLG49_07075"
     CDS             786..1151
                     /locus_tag="PLG49_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17118.1"
                     /translation="MSEETRMILKMVDDGKITPEQAVEMLKAVGQTESDREENDFRGD
                     DFIKDVEKWAKDFAAKMGNTVKDIEPKVKKIVKIMIQNTAKSIEEVGKVIKGQEEAFD
                     DDNDNNADSDFNEDEYTPN"
     gene            1400..3520
                     /locus_tag="PLG49_07080"
     CDS             1400..3520
                     /locus_tag="PLG49_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOQ17119.1"
                     /translation="MINEKHKVLLTEIFGSNVFNDATMRERLPRETYKALKKTIDEGA
                     NLDIKVAEVVANAMKDWAIERGATHYTHWFQPMTGMTAEKHNSFISPTSDGRIIMEFS
                     GKELIKGESDASSFPSGGLRATFEARGYTAWDCTSPAFLKEDANGVTLYIPTAFCSYT
                     GEALDKKTPLLRSMEALSKQALRILRLFGDTMTKKVIATVGAEQEYFLVDKKLFNKRK
                     DLMLTGRTLFGAPTPKAQEKEAHYYGTIKERVAAFMQELDVELWKLGVPVKIKHNEAG
                     PAQYEIAPLYSTANVAADHNQLIMETLKKIANRHDFACLLHEKPFAEINGSGKHNNWS
                     LATDEGKNLLKPGKTPHENLQFLLFLVAVIKAVDLYPELLRVSAANPGNDCRLGICEA
                     PPAIISIFIGEQLTEILEKIETGDSICSKEREILEIGVSTLPQVFKDTTDRNRTSPFA
                     FTGNKFEFRMVPSSASISDANVVINTIVADVLSDMADELEKAEDFNCAVYELLRKTIK
                     EHKRIIFNGNGYSEDWVKEAEKRGLPNIKSTVEAIPALISEKSIRLFEKHKVFTKSEL
                     CSRFEVLLEDYINTISLEAETMIQMAKREIFPAAVEYSTKVAQSITSVLNVGVYNVDA
                     QKDLLKELTDVIDSFNKNLKELEKHVQIAKSISTDSYEKACYYKDAVVSQMEKLRKDA
                     DHLECLVDEDIWPMPTYSELLFQI"
     gene            3589..4866
                     /gene="hflX"
                     /locus_tag="PLG49_07085"
     CDS             3589..4866
                     /gene="hflX"
                     /locus_tag="PLG49_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOQ17120.1"
                     /translation="MEKRNDNDYIHEEDYEKVILIAVEKENENAEENLEELEELALTA
                     KAITLDKIIQKRENVHPASYIGKGKIEEVKQKIYELNATGVICDDELSPAQMRNLEKA
                     LDCKVMDRTMVILDIFAQRASSKEGKIQVELAQLRYRLSRLAGSGEYLSRLGGGIGTR
                     GPGEKKLETDRRHIRNRIGQLKKELQELESHRELVRARRKKVGIPLVSIVGYTNAGKS
                     TLLNALSGADVLIEDKLFATLDPTTRKVKLPGGSEILLTDTVGFIRKLPHHLIEAFKS
                     TLEETQYGDILLHVVDSTAKNIEKKMETVYETLAKLKVLGKPVITLYNKCDKLTTGLL
                     PIDPKAEKTIKISAKNKEGLDELLQAIEEILKAGRKEIKALIPYSDIGILQLIRGEGE
                     RLIEEYRPEGIYIKANVNQRIYNKLEKYLIISN"
     gene            5083..5397
                     /locus_tag="PLG49_07090"
     CDS             5083..5397
                     /locus_tag="PLG49_07090"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HOQ17121.1"
                     /translation="MITKYLEGTRNCSTLDGEMIQLEYFLLEKEGTDGNNTVKTYGIE
                     IVKKSNFLGKAIRTETDQIDCLTHEKELAKEIINKLISHSVTPITMPYIINDMMDETP
                     CA"
     gene            complement(5426..5794)
                     /locus_tag="PLG49_07095"
     CDS             complement(5426..5794)
                     /locus_tag="PLG49_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17122.1"
                     /translation="MDNSQVLELILAKLQGMEEKIHGMESKIYGMEKKIETVGTDFDD
                     LKNGQEEIIINLKKLETKIENELTEKIRGLYDSREVIRDKLSSINIKVDNLSDRIDKH
                     ELIIKLAEDSNISSTKENIL"
     gene            6027..6707
                     /locus_tag="PLG49_07100"
     CDS             6027..6707
                     /locus_tag="PLG49_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOQ17123.1"
                     /translation="MLNQYKTVLQEAEAEIIEKKSKFIATVRPVKNEEEAQSFIEEMR
                     KKYWDATHNVFAYQIGERNQIQRFSDDGEPSGTAGLPVLDVLRGEDVKNVAIVVTRYF
                     GGTLLGTGGLVRAYGRAAKEGLLKAGVIEKKLYSRFHVITDYSWLGKVQYEILQGGHV
                     IEDTIYTDKIKLIVLVEEENKESFSKRIIEKTNAQAEISEVSMVYGAWNDGILKVEEI
                     DLDKEDNE"
     gene            6704..7081
                     /locus_tag="PLG49_07105"
     CDS             6704..7081
                     /locus_tag="PLG49_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOQ17124.1"
                     /translation="MSQELMKKKIWAVVGATTDPDKYGYKIYKALKEAGYKVYPISPK
                     YEEIDGDKAYKSLRDLPEKPEVVDFVVNPKIGIEVVKECAELGIENIWLQPGTVSEEI
                     LEYAKTHGINVVQACVLVALRTL"
     gene            7179..8102
                     /gene="cysK"
                     /locus_tag="PLG49_07110"
     CDS             7179..8102
                     /gene="cysK"
                     /locus_tag="PLG49_07110"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOQ17125.1"
                     /translation="MSKVYNSIDELIGNTPIVKLNKLVEEGSADVYVKLEWYNPGSSV
                     KDRIALSMINAAEQAGKIKPGDTIIEPTSGNTGIGLAMIGAARGYKVILVMPETMSIE
                     RRKLFKAFGAELILTPGTEGMTGAINKAKELVEEHGYFMPQQFENPANPEIHRNTTAK
                     EILEAMGTDLDAFVAGIGTGGTITGCGEVLKKAIPNIEIVAVEPAKSAVLSGEKKGPH
                     GIQGIGAGFVPEVLNTKIYDSIEKVDDEEALNYARRMAKEEGILVGISSGAAVAAALR
                     VAKRLGKGKKVLAISPSYGERYLSTALFSQE"
     gene            8224..8961
                     /locus_tag="PLG49_07115"
     CDS             8224..8961
                     /locus_tag="PLG49_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOQ17126.1"
                     /translation="MLKVIFFDLDNTLWNHNLALKKTIESVYDYLLENYSIPKDKAKF
                     IDMFNECNVKVWTEYKEGKISQKEVRINRFIDLLAFYGISDKDLVIKLDEFYISLYPT
                     WPVLFEGATELLEQLKGKYPLGLITNGFPITQEVKLKNANIKHYFNWIVYSEAVGKTK
                     PNKEIFDYALKEAGISGKEAVYIGDEFDVDIIGAKNAGMKTIWVRKQDNRSGKLPEIP
                     ATASLYADYMVTDLKEIVDIVKSIESC"
     gene            8997..9731
                     /locus_tag="PLG49_07120"
     CDS             8997..9731
                     /locus_tag="PLG49_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ17127.1"
                     /translation="MAGHSKFANIKHKKAKADAQKGKIFTKLGREIAVAVKEGGPDPA
                     NNSRLRDVIAKAKSNNMPSDTIERCIKKAAGEGDASNYEAITYEGYGPSGVAVIVEVL
                     TDNKNRTAANIRHAFTKGGGNLGTTGCVSFMFDKKGQIMIEKTDKIEEDELMMLALDA
                     GAEDFIADEEGYEILTSPEDFSAVREAIEKAGVEMISAEVTMIPQTTTKLTNEEDIKK
                     FEKMLELLEDDDDVQQVYHNWEQDED"
     gene            9917..10303
                     /locus_tag="PLG49_07125"
     CDS             9917..10303
                     /locus_tag="PLG49_07125"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17128.1"
                     /translation="MRYKNFFKKLNLKKDIVLDEDEEKAFHLVCVNQDELIGYCRLVV
                     NENIGYLSQIVVNEKYRGQGIGKQLILMNEKKALELGVSQIRLAAKVEVVEFYYKLGY
                     SKLGDVFPSKKTGLPHQLLIKKISGE"
     gene            10342..11499
                     /locus_tag="PLG49_07130"
     CDS             10342..11499
                     /locus_tag="PLG49_07130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOQ17129.1"
                     /translation="MTKKHIILWILILYLILILSACNKAEKTSAENEALIKEDQVIEN
                     TDEELKREENEEEVTQIPEAEEPQKIVIAFMGDVMMDSYIGDYIRDRGVDYPWEDVAS
                     ITQAADLAVINLETSVSTRGSTKKPEGYGFRSHPDSLQGLVNSGIDLVSLANNHSLDF
                     GEEAFNDTLDYLDQYGIAYVGGGKNKNEAEQLKIIEKNGIQLGFLAFTSVIPWSSWEA
                     TEDKAGTAVYKEEYKERLLQNIENASKECDILTIIPHWGVEYAQVPSEWQRELAHEMI
                     DAGADIIVGHHPHVLQGIEFYKEKPIFYSVGNFIFLKNDDLCGRTGIFQLTLDKDGFK
                     KGEFYPVNIQYCKANLLNKKEGRGKEIIDMLRELSASYGTIINEEGEIQKP"
     gene            11717..15499
                     /locus_tag="PLG49_07135"
     CDS             11717..15499
                     /locus_tag="PLG49_07135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOQ17130.1"
                     /translation="MNMSKVRRIFVEKKRGFDVEAQGMYQDLKENLEIKGLRSVRIIN
                     RYDIEGISDEEYLAARCTILSEPVVDHVYDENIDIDEHDKIIAVEYLPGQYDQRADSA
                     SQCIQILSQREMPVIRTAKLIVLSGDISDAEYSKIKNYCINPVECREATLAKPETLDE
                     KLSIPDDVKIINGFTTMSSEDLEKFMTEMGFAMSLDDLKFCQAYFRDTEKRDPTVTEM
                     RVIDTYWSDHCRHTTFLTCIDNVEIEEGPYKEVITDAYNRYLEARKNVYGEKQRDLCL
                     MDIATIAMKELRKRGLLDDLDVSDEINACSIKVNVDIDGQNEEWLVMFKNETHNHPTE
                     IEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSGDPRTPVKDTLPGKLPQRKITTGA
                     AAGYSSYGNQIGLATGLVEEVYDEGFVAKRMEIGAVIGAAPKKNVVRQKPEAGDVVIL
                     LGGRTGRDGCGGATGSSKAHTEESLLTCGAEVQKGNPLTERKIQRFFRNPKVSVMIKK
                     CNDFGAGGVSVAIGELADGLDINLDAVPKKYEGLDGTELAISESQERMAVVVAKKDAQ
                     TFIDAAKEENLEATEVAVVTEDMRMRMFWRGKVIVDISREFLNSNGAKQHTNVLVASP
                     DENNNVFCSLPKEVEEKLEDIEQAWLTNLQRLNVCSQKGLVERFDSTIGAGTVLMPFG
                     GSYQLSPQEGMAAKIPVLNGDTSIGTVMTYGYDPVVSKWSPFHGAAYAIIEAISKVVA
                     MGGNYKTIRLTLQEYFERLRKDPVRWGKPFSALLGSLYVQMKLGIPAIGGKDSMSGTF
                     KDMDVPPTLVAFAVDVVNVNHVISTEFKKAGSKVILLPVKRDKHQLPDFDQLERNWER
                     IYELACSGKILAAHTVRTGGIAEAISKMSFGNKIGFEFNGSMDVRSLFTADYGSMVLE
                     IADNENPDELFKGLEFKLLGTTVAEKVIKINGKSILLDKAIEKWEAPLEKIFPTKTQE
                     SRTNAALYSFSSPDRKKSSLKIAKPRIFMPVFPGTNCEYDTAKAFEKAGGQVDILVIK
                     NLTPSDVEESIKVMAQKIDNAQIIMLPGGFSGGDEPDGSGKFIATAFRNPYVKEAVMR
                     LLKQRDGLMLGICNGFQALIKLGLVPYGEIRDITEDCPTLTFNTIGRHVSCMVRTKVV
                     STLSPWFSKVSLGDIHTIAVSHGEGRFIANEDVLEMMKKNGQIATQYVDLDGNPTYDI
                     RFNPNGSIDAIEGITSPDGRVLGKMGHSERIGSNVAKNVPGEKDQKIFEAGVEYFS"
     gene            complement(16068..>16392)
                     /locus_tag="PLG49_07140"
     CDS             complement(16068..>16392)
                     /locus_tag="PLG49_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17131.1"
                     /translation="LLHQSGDYLSVSIQMHTFLHYYLHANSFFISLLFKMNLSKNKIK
                     ASLNFSAYLEKKYGKAKDLPVHTRSLINKRFKKAPYDYSTSALVMLSCRIKFTVCLTN
                     YNICL"
BASE COUNT         5891 a         2409 c         3488 g         4604 t
ORIGIN      
        1 ttgattctct tgactggaag gaatatgggg tagaccctat gataaaacaa gttctcaatc
       61 ataagcattt aggcaatggt tctattattc tgtttcataa caatgcaaaa tacaccaagg
      121 atgccttaga tgctattata actggcttaa aagagcaggg ctatgaaatg gtacccatat
      181 cccagcttat cttaaaagat aattattaca ttgaccatga aggtcgccag agaccaataa
      241 aagataataa gcaggccaga aataaatagt atatataaag ggacattgaa aagatgtccc
      301 tttattattc atttaatcct tgggctctgt acagttgctt tgttcttcta ttaatttatt
      361 tattaaagaa tcaatttttc tactcagata ttgaacaagg ggatctaaaa aattatagtt
      421 tctttgtctt agcagggttt ctaaagcagc ttttaaaatt tcaatttctt ctgttgtatt
      481 tttgttttcc tgacccataa ataccccctc ttttctaata ctgtttgtca tagcggagga
      541 tgattgatta aattatttta tatactttag tttatgacaa aaatatcttt aagtgattta
      601 aaataaggat tttaaaaaaa gtaaaatcat tattatatta cccaaataaa ataagctgat
      661 tcttaatata gaaaaaataa tattaatgaa aagaaaataa aagcaaacaa aatataaaaa
      721 gaaacgtata tattagagaa aataagcaat caataaaaat aagataaatt tagaggagga
      781 tttatatgag tgaagaaacc agaatgattt taaagatggt tgacgacggg aaaattacac
      841 cagaacaggc agtagaaatg ttaaaagcag taggtcaaac tgaatctgac agagaagaaa
      901 atgactttcg tggagacgat tttataaaag atgttgaaaa atgggcaaaa gattttgcag
      961 caaagatggg caatactgta aaagacatag aacctaaagt taaaaagatt gtaaaaatca
     1021 tgattcaaaa cacagcaaaa tccattgaag aagtcggaaa agttattaaa ggtcaggaag
     1081 aagcgtttga cgatgacaat gataataacg ccgacagtga ttttaatgaa gatgaatata
     1141 ctccaaatta aaaggacggt aaaaagccgt tcttttattt tttcagttta gtaggatgat
     1201 gaggcattta atttggaagg gaatatcccg cgctttccta ttttaagttt tacttgtaaa
     1261 gaataatatt tcatgataaa atttaaaatg attactaact ggaaaagtcc ggcgttctca
     1321 aaggcatgtt tggggacgcc ttttataaat aaattaagct ggacatgcca ttatttatat
     1381 acatacagga ggttgttgaa tgataaacga aaaacataaa gtacttttaa cagaaatatt
     1441 tggttctaac gtatttaatg atgcaacaat gcgggaaagg ttgcccaggg aaacttataa
     1501 ggcgttaaag aaaaccatcg atgaaggtgc caatctggat ataaaagtag ctgaagtggt
     1561 tgctaatgcc atgaaggatt gggcaattga aagaggagca acccattata ctcattggtt
     1621 ccagcctatg acagggatga cagcggagaa gcataattct tttatttcac caacctccga
     1681 tggacgcatt attatggagt tttcagggaa agaattgata aaaggagaat ccgatgcttc
     1741 ttctttccct tcaggaggac ttagagcgac ctttgaagca agaggttata cggcgtggga
     1801 ttgtacttct cctgcttttc ttaaggaaga tgctaatggg gtgactttgt atataccaac
     1861 ggctttttgt tcttataccg gggaagccct ggacaaaaaa acacccctgc ttagatctat
     1921 ggaggcccta tccaaacagg ctctgagaat cttaagatta tttggggata ctatgaccaa
     1981 aaaagtgata gctacagtag gtgcagaaca agaatatttc ttagtggata aaaaattatt
     2041 taacaaaaga aaagacttga tgttaacagg aaggacctta tttggggcac ctacaccaaa
     2101 ggctcaggaa aaagaagctc attattatgg aacgattaaa gaaagagtag cagcttttat
     2161 gcaggagttg gatgtagagc tttggaaact tggcgtacct gttaagatta agcataatga
     2221 agcagggcca gctcagtatg aaattgctcc gctttacagt acagcaaacg ttgcggcaga
     2281 tcataatcaa ttaattatgg aaaccttaaa aaagattgca aaccgccatg attttgcatg
     2341 tcttttgcat gagaagcctt ttgcagaaat taatggttct ggtaaacata ataactggtc
     2401 ccttgcaacg gatgaaggca aaaatttact aaaaccaggt aagacgcctc atgaaaatct
     2461 ccaatttttg ctgttcttag ttgcggttat taaggctgtt gatttatatc ctgaattact
     2521 aagggtttct gctgctaacc ctggtaatga ttgcagactg ggaatttgtg aagcgccacc
     2581 agcaattata tctattttta ttggagaaca attaacagag atattagaaa aaatagaaac
     2641 aggagacagt atttgttcca aagaaagaga aatactggaa ataggggtat caaccctgcc
     2701 acaggtattt aaagatacca cagaccgtaa cagaacttct ccttttgcct ttacagggaa
     2761 taaatttgag tttagaatgg taccttcttc agcttctata tccgatgcaa atgttgtcat
     2821 caatacaata gtggcagatg ttttgtcaga tatggcagat gaacttgaaa aggctgaaga
     2881 ttttaactgt gccgtatatg aattattaag aaaaacaatt aaagaacata aaagaataat
     2941 ttttaatgga aatggttatt ctgaagattg ggtaaaagaa gctgaaaaaa gaggtcttcc
     3001 caatattaag tcaaccgtag aagctatacc tgcccttatt tcagaaaaat ccatccgctt
     3061 atttgaaaaa cataaagtct ttacaaaatc tgaactctgc tcaagatttg aagtcctttt
     3121 ggaagattat atcaatacta ttagtcttga agcagaaacc atgattcaaa tggcaaaaag
     3181 agaaatattc ccggcagctg tggaatattc aacaaaagta gcacaatcca ttacatcggt
     3241 tttaaatgtg ggcgtttaca atgtggatgc tcaaaaagac ttacttaaag agctaacaga
     3301 tgtaatagat tcctttaaca agaatttaaa agaattagaa aagcatgtac aaatagctaa
     3361 aagcataagc acggattctt atgaaaaggc atgttattat aaagatgccg tggttagtca
     3421 aatggagaaa ctcagaaaag atgcagacca tttagaatgc ttagtcgatg aagatatttg
     3481 gccaatgccc acttatagcg aactgctttt ccaaatataa taaaacggtt tagtaccgtt
     3541 ttattttttt aaatagcttt tatattaatt aaaaaaagga gaatgacttt ggaaaaaaga
     3601 aatgacaatg actatatcca tgaagaagac tatgaaaagg taattcttat tgcagttgaa
     3661 aaagagaatg aaaatgcaga agagaacctg gaggagttag aggaattggc gttgactgca
     3721 aaggcaatta ccttagataa aattattcaa aaaagagaaa acgtgcaccc tgccagctat
     3781 attggtaaag gtaagattga agaggttaaa caaaaaattt atgaattaaa tgcgacgggt
     3841 gtgatttgtg acgatgaact ttctcctgca caaatgagaa atcttgagaa agccttagat
     3901 tgtaaagtaa tggacaggac gatggtgatt ttagatatat ttgctcaaag ggctagttct
     3961 aaggaaggaa agattcaagt agagcttgct cagcttaggt acagactgtc aagactggca
     4021 ggttccggag agtatctttc cagacttgga ggaggtattg ggacaagggg accaggtgaa
     4081 aagaaattag aaacggacag gcgacatata agaaatcgaa ttggtcagtt aaaaaaagaa
     4141 ttacaagagt tagaaagcca cagagaactt gtaagagccc gaaggaaaaa agttggaata
     4201 cctttagtca gcattgtggg ttataccaat gcgggtaagt ctactctttt aaatgccctt
     4261 tcaggagcag atgttttgat agaagataaa ttgtttgcaa cccttgatcc gactacccga
     4321 aaggtaaaac tgccaggagg ctcggaaatt ttgcttactg atacagtcgg atttatcagg
     4381 aaactgccgc atcatttaat tgaggcattt aaatctactt tagaagaaac acaatatggt
     4441 gacatattgc ttcatgttgt tgacagtaca gctaaaaaca tagagaaaaa gatggagact
     4501 gtatatgaga ctttagctaa acttaaagtc ttagggaagc ctgtcattac tctttataat
     4561 aagtgtgata aattaacaac cggtcttctt cctatagacc ctaaggcaga aaaaactata
     4621 aaaatatctg caaaaaataa agaaggcctg gatgaactgc tacaggccat agaagaaata
     4681 ttaaaagcag ggaggaaaga aatcaaagct ttaattcctt attcggacat aggtattctt
     4741 caattgataa gaggagaagg agaaaggttg attgaagaat acagacccga gggaatttat
     4801 ataaaggcca atgttaatca aagaatttat aataagttag agaaatatct tataattagt
     4861 aactaataat tattcttata aattatcgca aatacttaag gcatttaata taattcgaca
     4921 gcgaatttat ttattaattg atgatgttgt cgaaattata taaaacttat taaatattgc
     4981 gaaatttaac actagtcttt ttttaaaaaa ttctgtataa taaaacccgt aagaattcaa
     5041 cattcattat gttcaaataa atattttacg gggggtagtg taatgatcac gaagtattta
     5101 gagggaacaa gaaattgcag tacactggat ggcgaaatga ttcaattgga atactttctc
     5161 cttgagaaag aagggacgga tggtaacaat acagtaaaaa cctatgggat agaaattgta
     5221 aaaaaaagta attttctcgg gaaagccatt cggactgaaa cggatcagat agactgcctt
     5281 actcatgaga aagaattagc aaaggaaatt attaacaaat taatctctca ctctgttaca
     5341 cccatcacca tgccatacat cattaatgac atgatggatg aaacaccttg tgcataaaaa
     5401 gcaaggtgtt ttttttaatt aaaaattaaa gaatgttttc tttagtagag ctaatattgg
     5461 agtcttctgc cagctttata attagttcat gtttgtctat ccggtcggaa agattgtcta
     5521 ctttgatgtt aatactactc agtttatccc tgatgacttc ccgggaatca taaagtcccc
     5581 ggattttttc tgtcagttca ttttctattt ttgtttctaa ttttttaaga ttaataatga
     5641 tttcttcttg tccatttttg aggtcatcaa aatcagtacc aacggtttca attttctttt
     5701 ccatgccata aattttactt tccattccgt ggattttctc ttccataccc tgaagcttgg
     5761 caagaattag ctcaagtact tggctgttat ccatttatag ccctccccat atatgcttac
     5821 taactaacta tatagtacta ttatttttcg aataggtaaa tatatagtta tatagatcct
     5881 atcaaatttc ctttgtcttt taaaggcccg gacattatta aaaatacagt ttaaaataca
     5941 aaaatcattt tttattaata tctgatgaga tatgatataa ttatttaaaa taaaaattat
     6001 tagatattta actaaggagt atgcttatgt taaatcaata taaaactgtt ttacaagaag
     6061 cagaagcaga aattatagaa aaaaaatcaa aatttattgc aacggttaga cctgtaaaaa
     6121 atgaagaaga agcacaaagc ttcattgaag aaatgcggaa gaaatactgg gatgcaaccc
     6181 ataatgtttt tgcgtatcaa attggagaga gaaatcaaat acaacgattc agtgatgatg
     6241 gagagcctag tggaacagca ggacttccgg ttttagatgt gttaagaggt gaagatgtaa
     6301 aaaatgtagc gattgttgtt acaaggtatt ttggaggaac cttattgggg acgggaggcc
     6361 ttgtaagagc ctatggccgg gcagcaaaag agggtctctt aaaagcgggt gtaatcgaga
     6421 aaaaacttta cagccgtttt catgtcataa cggactattc ttggcttggg aaagtccagt
     6481 atgaaatttt acagggcggt catgtgattg aagatactat ttataccgat aaaataaagt
     6541 taatcgtttt ggttgaagaa gagaataaag aatcatttag taagcgaata atagagaaga
     6601 ccaatgcaca ggcagaaatc tcagaagtca gcatggtgta tggggcatgg aacgatggga
     6661 ttttaaaagt cgaagaaatt gatttagata aggaggataa tgaatgagcc aggaattaat
     6721 gaagaaaaag atttgggctg ttgtgggggc aacgacagat ccagataagt atggttataa
     6781 gatttacaaa gctttaaaag aagcgggtta taaagtttat ccgattagcc cgaaatatga
     6841 agaaatagat ggtgataaag cctacaaaag tttaagggat ttaccagaaa agccagaggt
     6901 agtggatttt gtagtcaatc ctaaaattgg tattgaagtt gtaaaggaat gtgccgaact
     6961 cggaatagaa aacatttggt tacaaccagg aactgtcagt gaagaaatat tagaatatgc
     7021 aaaaacgcat ggtataaatg tcgtgcaagc ttgtgtttta gtagctcttc gaacattata
     7081 aaaatatttt aaaaaaggtt gacatcagtt taataactat agtaaaataa aatcataaaa
     7141 accgataaga ataatatgaa ttaaaaagga gtgattatat gagtaaggta tacaattcta
     7201 tcgatgagtt aattggtaat acacccatcg ttaaacttaa taaattagta gaagaaggca
     7261 gtgcggatgt atatgtaaaa ctggaatggt acaatccagg cagcagcgta aaagacagaa
     7321 ttgcattaag tatgattaat gcagcagaac aagcaggaaa aattaagcca ggagatacaa
     7381 tcattgaacc taccagtggg aatacaggca ttggtttagc catgatagga gcagccagag
     7441 gttataaagt tattttggtt atgcctgaaa ccatgagtat agaaagaaga aagcttttta
     7501 aagcctttgg tgcagaactt attttaacac ctggtacaga aggtatgact ggagccatca
     7561 ataaagctaa agagttagtt gaagaacatg gctattttat gcctcaacaa tttgaaaacc
     7621 ctgccaaccc agaaattcac cgcaatacaa ctgctaaaga aattttagaa gctatgggaa
     7681 cggatttaga tgcttttgta gcaggcattg gaactggcgg aaccataaca ggttgcggag
     7741 aagtcttgaa aaaagctatt ccaaatattg aaattgttgc tgtagaacct gcaaaatctg
     7801 ctgttctttc tggagaaaag aaaggacctc atggtatcca gggaatagga gcaggtttcg
     7861 ttccagaagt tttaaataca aagatttacg attccattga aaaagtagac gatgaagaag
     7921 ccttgaatta cgcaagaaga atggcaaaag aagaaggaat tttagttggt atttcatcag
     7981 gagccgcagt ggctgcagcg cttagagtag caaaaagact tgggaagggt aaaaaagttt
     8041 tagcgatctc tccaagttat ggggaaagat atttaagtac cgcattattt agccaggaat
     8101 aagaaaaaat aaaagggaca tcaattgtcc cttttttatt tgtagatttt tataattaag
     8161 tatacaataa aataaaggaa gcacggggac ggttcctgaa aaacttttga aaggaagtca
     8221 aaaatgctta aagtaatctt ttttgatctg gataatacct tatggaatca taatttagca
     8281 ctcaagaaaa ccatagaaag tgtttatgat tatctgctag aaaattattc tattccaaaa
     8341 gataaagcta aatttatcga tatgtttaat gaatgtaatg taaaagtatg gactgaatat
     8401 aaagaaggga aaataagcca aaaagaggtc cgtatcaatc gttttattga tttattggcc
     8461 ttttacggca tttccgataa agatttagtt attaaattag atgaatttta tatttcactt
     8521 tatcccacat ggcctgtttt atttgaaggg gcaacagaat tattagagca attaaaaggc
     8581 aaatatccac taggacttat taccaatggt tttccgataa cacaagaagt aaaattaaaa
     8641 aacgccaata taaaacacta ttttaattgg attgtttatt ctgaagccgt aggaaaaaca
     8701 aaaccgaata aagaaatttt tgattatgcc ttaaaagaag cgggcatttc tggtaaagaa
     8761 gctgtctata ttggagacga atttgatgta gacataattg gtgcaaagaa tgctgggatg
     8821 aaaaccattt gggtcaggaa gcaagataat cgctcaggta aactgccgga gattcctgcc
     8881 accgcttctc tttacgcaga ttatatggtc acggacttga aggaaatcgt tgatattgtc
     8941 aaatccatag aatcatgcta aaatatcaac gatatgaaat gttaggagga aattgaatgg
     9001 caggtcattc aaagtttgcg aatattaaac ataaaaaagc aaaagcggat gctcaaaaag
     9061 gaaaaatatt cacaaagctg ggcagagaaa ttgcagtagc cgtaaaagaa ggcggtcctg
     9121 atcccgccaa caattcaaga ctacgtgacg tgattgcaaa agcaaaatca aataatatgc
     9181 caagtgatac catagaaaga tgtattaaaa aagctgctgg tgaaggtgat gcatctaact
     9241 acgaagcaat cacttatgaa ggatatggac caagtggtgt agctgttatt gtcgaagtgc
     9301 tgacagataa caaaaaccgc acagcagcca atatccgtca tgcttttaca aaaggcggcg
     9361 gcaatttggg aacaacaggt tgtgtatcct ttatgttcga taaaaaagga caaatcatga
     9421 tagaaaaaac agataaaatt gaagaagatg aattaatgat gttggcactt gatgcaggtg
     9481 cagaagattt tattgcagat gaagaaggct atgaaatctt aacatcacct gaagatttct
     9541 cagcagtcag ggaagctatt gaaaaagcag gagtagaaat gatttctgct gaagtaacaa
     9601 tgattcccca gacgaccact aaacttacca atgaggaaga tattaagaaa tttgaaaaaa
     9661 tgttagaatt acttgaagac gatgacgacg tgcaacaagt atatcacaac tgggaacaag
     9721 atgaagacta atcaattaaa tacttggcga gaattaaaac ctccagtcat aatttatcat
     9781 gattggaggt ttttttggaa gtaaagcact atcctttact cccttgttaa aatgttaaga
     9841 gaggattgga ttatataaga tgtatgaata taagtttatt agccgggagg attcaattta
     9901 tcagcaggtt ttggacttgc gttacaaaaa tttttttaaa aaattaaact taaaaaaaga
     9961 tattgttctg gatgaagatg aagaaaaagc ttttcatctg gtttgtgtga atcaagatga
    10021 gttaattggt tactgcaggt tggtggtcaa tgagaatata gggtatcttt cacaaattgt
    10081 agtaaatgaa aaatacaggg gacaaggtat tgggaaacaa ctaattctta tgaatgaaaa
    10141 aaaggcactg gaactgggtg tttctcagat aagactggct gcgaaggtag aagtagtaga
    10201 attttattat aaattaggtt attccaaatt gggagatgtt ttcccttcta aaaaaacagg
    10261 attaccccat caactgctta ttaaaaagat atcgggagag taggaaacgt tttcaaaaat
    10321 gacttaaacg gaggaaatgt aatgactaaa aagcatatca ttttgtggat acttatttta
    10381 tatttaatcc ttatattatc tgcttgtaat aaggccgaaa aaacttcagc agaaaacgaa
    10441 gcgcttatta aagaagacca ggtcattgaa aatacagatg aagaacttaa aagggaagaa
    10501 aatgaagaag aagttactca aattcctgaa gcagaagagc ctcagaaaat tgttattgct
    10561 tttatgggag atgtcatgat ggacagctac ataggagact acatccggga cagaggggtt
    10621 gattatccct gggaagatgt agcaagtatt acccaggcag ccgatttggc tgtgattaat
    10681 ttagaaacca gtgtcagtac aagaggctca accaaaaagc cggaaggcta tggtttccgt
    10741 tctcatcctg acagtctgca gggtttagtg aacagcggta tagatttagt ttctcttgcc
    10801 aacaaccaca gcttagattt tggagaagag gcttttaatg acactttaga ttatctggat
    10861 cagtatggta ttgcctatgt gggtggcggc aaaaacaaaa atgaggcaga gcaattaaaa
    10921 ataatcgaaa aaaatggaat acaattaggc tttttggcct ttacctcggt aattccctgg
    10981 agcagttggg aagcaactga agacaaagct ggtacggcag tttataaaga agaatacaag
    11041 gaaagattac ttcaaaacat tgaaaatgca tcaaaggaat gtgatatatt aacgatcatc
    11101 cctcattggg gtgtagaata tgctcaggta cccagtgaat ggcaaagaga actggcccat
    11161 gaaatgattg atgcaggtgc ggatatcatc gtaggtcatc atcctcatgt tcttcaggga
    11221 atagaatttt ataaggagaa gcccatattc tacagtgtag gtaattttat ctttttgaag
    11281 aatgatgatt tatgcggtag gacaggaata tttcaattga cattggataa agatggtttt
    11341 aaaaaaggag aattttatcc agtcaatatt caatactgta aagccaactt attaaataaa
    11401 aaagaaggaa gaggaaaaga aattattgat atgctaagag aactgtctgc ttcttatgga
    11461 accataataa atgaagaagg agaaattcaa aaaccataaa ataaaacagg gggacggttc
    11521 cttgtttcat gctcaaacgc gaaacaagga accgtccccc tgttttatta gtacaactaa
    11581 taaattctat taaaattatt aattttaatt atttatgata tcgggttata attaaactat
    11641 acagaaaata ttgtataatt atgttgaata acggtcaatt aaggcgctgt ctttaattat
    11701 tgtgtgtgaa gggagaatga atatgagcaa agtcagaagg atttttgttg agaaaaaaag
    11761 gggctttgat gttgaagccc agggcatgta ccaggatttg aaggagaacc ttgagatcaa
    11821 agggctgcgt tcggtcagga taatcaacag atatgatatt gaggggattt cggatgaaga
    11881 atatcttgca gcacgctgta ccatactttc tgaacccgtg gttgaccatg tatatgatga
    11941 gaatatagat attgatgaac atgacaaaat catagcagta gagtatctgc caggacaata
    12001 tgaccagagg gccgattctg cttctcaatg tattcagata cttagccaaa gagaaatgcc
    12061 tgttataaga actgcaaagt tgattgtact gagtggagat atatccgatg ccgaatattc
    12121 aaagataaag aattattgta taaaccctgt tgaatgtaga gaggccacat tagcaaaacc
    12181 tgaaacgctt gatgagaaat tgtccatacc cgatgatgta aagataataa atggttttac
    12241 gactatgtct tctgaggatc ttgagaagtt tatgactgag atgggatttg caatgtccct
    12301 ggacgacctt aaattttgtc aggcatattt cagagatact gaaaaaagag atcctactgt
    12361 cactgagatg agggttatag atacctattg gtctgaccac tgcagacata caacctttct
    12421 gacttgtata gacaatgtgg agattgaaga aggaccttat aaagaggtta ttactgacgc
    12481 atataaccgt taccttgaag caaggaagaa tgtttatggg gaaaaacaga gagatctttg
    12541 tctgatggat attgcaacca ttgccatgaa agagctcaga aaaagaggac tgcttgatga
    12601 tctggatgtt tctgatgaaa taaacgcatg cagcattaag gttaatgtgg atatagacgg
    12661 acaaaatgaa gaatggctgg ttatgttcaa gaacgaaacc cataaccacc caaccgaaat
    12721 agagcctttc ggaggagctg ctacatgtct tggaggagca atacgtgacc ctctgtctgg
    12781 caggtcatat gtttatcagg ctatgcgtgt gactggtagc ggcgatccaa ggacgcctgt
    12841 taaagacacc ttgcccggaa aacttcctca gaggaagatt actacaggtg ctgcagcagg
    12901 ttacagttct tatggaaacc agataggact tgcaacagga cttgttgaag aagtgtatga
    12961 cgaaggattt gtggcaaaaa ggatggaaat tggtgctgtt attggtgctg cgccgaaaaa
    13021 gaacgtagta agacaaaaac ctgaagcagg tgatgtggta atccttcttg gtggaaggac
    13081 aggcagggat ggttgcggag gagcaacagg ttcatcaaag gcacatactg aagaatctct
    13141 gcttacttgc ggagcagaag ttcaaaaagg aaatcctttg acggaaagaa aaatacaaag
    13201 attttttaga aatccaaaag taagtgtaat gataaaaaaa tgtaatgact ttggagcagg
    13261 gggcgtatct gttgccatcg gtgaattggc ggacgggctg gatataaatc ttgatgctgt
    13321 accaaaaaaa tatgaaggat tggatggaac cgagcttgcg atatccgagt cacaggaacg
    13381 catggctgtt gtcgttgcca aaaaagatgc tcagacattc atagatgctg caaaagaaga
    13441 aaaccttgaa gctacagaag tggcagtagt gaccgaagac atgagaatgc gtatgttttg
    13501 gagaggaaaa gttattgtag atatcagcag ggaattttta aattcaaatg gagcaaagca
    13561 gcatactaat gtattagtag cttcacctga tgaaaataat aatgtttttt gttcattgcc
    13621 caaagaagtg gaagaaaaac tggaagatat agaacaagcc tggcttacta atcttcaaag
    13681 acttaatgtc tgcagccaaa agggattggt ggaaagattc gacagtacga ttggtgcagg
    13741 aacagtactt atgccttttg gaggaagcta tcaattgagt cctcaggaag gaatggctgc
    13801 taagattcct gttcttaatg gcgatacatc tatcggaaca gtgatgactt atggatatga
    13861 tcccgttgtt tcaaaatgga gtcctttcca tggggcagca tacgccatca ttgaagctat
    13921 ttctaaggtt gttgccatgg gcgggaatta taaaaccata cgccttaccc ttcaggaata
    13981 ttttgagagg ctgcgcaagg atcctgtcag atggggtaaa cctttcagtg ctcttttggg
    14041 atctctttat gtccagatga agctgggtat acctgccatt ggagggaaag acagcatgtc
    14101 aggtactttt aaagatatgg atgtacctcc aacgctagtg gcttttgcag tagatgttgt
    14161 gaatgtaaat catgtgatat caaccgagtt taaaaaggca ggaagtaaag ttatattgct
    14221 tcctgtaaaa agggacaagc atcagttgcc tgattttgat cagttggaaa gaaattggga
    14281 acgtatttat gagcttgcct gctcgggaaa aatccttgca gcccataccg taaggactgg
    14341 aggaattgct gaagcgatta gtaagatgag ttttggaaat aaaattggtt ttgagtttaa
    14401 tggttcaatg gatgtaagaa gcttgtttac tgctgattac ggttcaatgg tattggaaat
    14461 agccgataat gaaaatcctg atgagctttt caaaggactt gagttcaaat tactgggtac
    14521 gactgttgcg gaaaaagtca ttaagattaa cgggaaatca atccttctgg ataaggcaat
    14581 cgaaaaatgg gaagcacctt tggaaaaaat attccccacc aaaacgcagg aaagcagaac
    14641 aaatgctgca ttatattcat tcagcagtcc agacaggaaa aaatcgagct taaagattgc
    14701 aaaaccacgt attttcatgc cagtgttccc gggaacaaac tgcgaatatg atacagccaa
    14761 agcttttgag aaagctggag gacaggtaga catactggtg atcaaaaacc ttactccaag
    14821 cgatgtagag gagtctataa aggttatggc gcaaaaaata gataatgctc agatcattat
    14881 gcttcccgga ggctttagtg ggggtgacga gcctgatgga tcaggtaagt ttattgccac
    14941 cgctttcagg aatccttatg taaaagaggc ggttatgcga ctccttaagc agcgggatgg
    15001 actgatgctg ggaatttgca atggttttca ggctcttata aaacttgggc ttgtaccata
    15061 tggagaaata agagatatta ctgaggattg tcctacactg acctttaata ccataggccg
    15121 ccacgtatcc tgtatggttc gtaccaaagt ggtatccacc ttgtcaccct ggttttctaa
    15181 ggtatccctc ggggatattc acaccatagc tgtttcacat ggggaaggca gattcattgc
    15241 taatgaagat gtactggaaa tgatgaagaa gaatggccag atagcaacac aatatgtgga
    15301 cctggatggt aatcctactt atgatataag gtttaatcca aatggctcaa ttgatgccat
    15361 agaaggaatc acaagccctg acggaagggt gcttggtaaa atgggccatt ctgaaagaat
    15421 cggttccaat gtggctaaga atgtgcccgg agaaaaagat cagaagatat tcgaggcagg
    15481 agtagaatac tttagctaaa aaggggtggt tcttctgctt ccgttacaga atgcataaag
    15541 gggagaaacc tatgattcta atcaatgcag aagctccata cagagtttga ggagactgca
    15601 tggagcgttg agccgttcaa aggctgttat cctcagactt tgcacttttg atacaaaaaa
    15661 attaaaatca aaggagcaaa gtcaaaatga aaaatagaag atatgagtta aaaaatttct
    15721 atattttgtg gagcacacgc ggatataaaa aaccaccttt aatcaaaaat gattaaaggt
    15781 ggttttttat aattcttgtt ttaaaacagc agatagatta tcataaaaaa acagaggtaa
    15841 cagggaagaa gcaaaagaac tgttctaaaa attaaagtaa gtaattaaaa aattattgaa
    15901 atggattatt aattaaaata taataaaatt acttttttgc taatattaaa acaaatttta
    15961 aagataaaaa aacaaaagca aaatgagctg atcttacgag acagcaccta tcatttactg
    16021 ataagagcaa aattagtcga tgagctaaaa atgcactcaa aagcatttca gaggcaaata
    16081 ttataattag taagacagac ggtaaatttg atccgacaag ataacattac aagagcagaa
    16141 gtactgtaat cataaggtgc ttttttgaat cttttattga ttaaagaacg ggtatgcaca
    16201 gggaggtctt ttgctttccc gtattttttt tcaaggtaag ccgaaaaatt taagcttgcc
    16261 ttgattttgt ttttggaaag attcattttg aaaaggagtg atataaaaaa tgaatttgca
    16321 tgcaagtaat aatgaagaaa agtatgcatt tgaatggaca ctgacagata atcaccagat
    16381 tgatgcaata gc
//
LOCUS       DAOSUD010000047        16252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2203_length_16252_cov_26.713898, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000047 DAOSUD010000000
VERSION     DAOSUD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_16252_cov_26.713898"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..2604
                     /locus_tag="PLG49_07145"
     CDS             208..2604
                     /locus_tag="PLG49_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17132.1"
                     /translation="MGFYIERKRGLFLIFILLLIVCIIPQNLQAAEKKIEALENNGAK
                     VTDIWQKGQWSFTIRGTGLTDNIKEIRLIRVNDALVITIDKKDIQKKNDQELVVNITG
                     ENAKKFSSEQYTGLYDIEIEYLDGSVLSPAESYNSIKMTVKGQNFKEDIEFVELKNLK
                     TGAWEIILAKNKVQYKDENTLILTLDKDEVKKLAGGINASDIEMTVYYGKDYIFDNKQ
                     SYFDIYLLGENFNRGVEKVILRPIAGEALLLPVDEQDIIMEKEDISVVNGSKLRVRIR
                     RDNLDKIKEFRHSGDYKFVVVYTAGTGLKDYTASMDLKLVYNYGLQTKESVVYRDNLV
                     FDNKDYITDPFRFVLLSKSSPKVIEVYPQSVGGYPWFNEKNLSHEILKDRNFLKITFE
                     DIDGKLEFNPYLGIDNLLGSSVMAIGSNSDFLDRDFIILCRDNPEMIHTYLFKQDRTN
                     QKAYLYIPVKPLVPQTQYVVNISGNIVRNDSSDNGMIDEGQRYNKPISWEFSTMASPS
                     VSEEGVLVQTVIEGYASSSYIKIYGDDFYKSSVRVFFNDTQADQVLVEQDNNGKSYLK
                     VYLPTGSRRLKAGLYNIIIKNDSDHETVIYGGLSVVEKGDEIPTEGYHLKTRTVEGEV
                     LSDAYVSHDTLYLRSKYSDYYLLELNLDKLMGEEVLNKTVNYIGNRNDYINRLLLKTK
                     DVDATFYYLTLKENASSKEININIGRAKPLVVQAIKDKLKDKKIKSEFIEISTSNCNW
                     DNLYISIPYKNSSGENLSIYRYDEIRRTWTKEDSYTDLINQKIRAFVKKAGIYVVIED
                     "
     gene            2640..>3215
                     /locus_tag="PLG49_07150"
     CDS             2640..>3215
                     /locus_tag="PLG49_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17133.1"
                     /translation="MRKKLKRYLAFLMVLIFNISLLPSDFVYAFDMNNFSITSITIGK
                     TYDRNRIFNNAYITITGKHLKDASVGIITYGEGYIPLGTPKVNSDGILQFEISENQLG
                     EFLSIEGITIPINEANMPNLTDISRNVKIGTDDLVIRGTRLNNVDGTNIVARYGTGNS
                     YTTLGAFSNATEVTISKPSGSLGLHEFIFSSS"
     assembly_gap    3216..3315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3316..8959
                     /locus_tag="PLG49_07155"
     CDS             <3316..8959
                     /locus_tag="PLG49_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOQ17134.1"
                     /translation="ISKPSGSLGLHEFIFSSSFKVYDIDFNDNNQKKSVDIQINYTYK
                     DQFRFITDLAIEGDLNMFPNRGEAGDIVYFTADKLDEYDVFFLKDLSGTDLYSNANKG
                     VNTTYKAKSSSDEKDTLTTQVPKLPVGEYYVVLTNKISSGKDPMKEVTGERVLAEKFT
                     IIDGSNKAVIVDVQPNKGPDTGELVTISGQFLGSLNISEFTPSQDSTLTVNQNSSPEE
                     LEIDYGQGTYGPSQIKIVSAKRIIRVIIGNKAAFVKDNGEYQVFFNKDLDKIIVRTAQ
                     VTDADINPVRDVVIETETVLTKEDGSKIVFKERAVKEKGYTFIVSKIKPEITEAVPNK
                     IQVVNNGGTFQIPSDRYIAIYGKNFMIHKYTNEDGREVIRYPIVQLGPNLIIDKNNPA
                     SDFNDPGKSLKGKDEVYLKVFDAYGRELDGSSGNEMGTKILIKLEEGSPAAGVVLGKT
                     FIKVINPMRNSRETGLETQMNDFIEFVNPETSKNPIIQSVNPNVVSVEGGEEVIVTGS
                     NFTDGVKVFIDGKEVTPINRGADGKTITFKAPAGREGQTQLQVMNIEGGMDVYPFSYV
                     KTFTNPKITSIEPRKGNTGTLVVVKGENFLKPDPTGGESAIYRLIGTRILLEGEDINS
                     YNINTATKEIELVAFTPNTPLISVQNSRIQVASYYHSILLRAPGGKLYTLDVDYSGVP
                     VISDGIGNDYKIVYNVQSGKLQADKAGGGLYDLTVSGGNITIQQDSGLSLQIVTPFEV
                     KDGEVIGDLVKVVDKNTIYFTVPILPADGWYDVTVQNPDTKKDTKKDKDGFYYYKQPQ
                     STPVIDEVVPSEGSVAGGNVVTIKGKEFMDNGFEKVRVFINGVEVAPANIQVSTTSDS
                     ITVVVPPYSGDLMAEKGVGRLSVPVVVINPDGGSASKENAYTYVIPSSNPKIERILPT
                     SGTAAGGAVVEIFGSDFRTFEPYEDYNRNQIRDENEPYRDLNNNGQHDMTFDPEKDKK
                     ELDHPLYSYYYDSPLLPKIYFGMEQALIVEFSATYIKVVTPRGSGTVPVFIENNDSGR
                     SNTVYYSYQGSKPKITKVSPGVGNRMGNTNIGIYGEDFRQSNIKVYKSVSEKETRTMT
                     LVRFGDNTNKNIPRDQANSGLINQGNAQVSLEKDFTAIYKAEYDDTYDSFSEIRVVIK
                     YNDVIYSNTFKRFNDEESFINTNLLVDSDGNRYPFSELIRFAVEDRRFIVERGYAPQV
                     IYISSGELSVTIPPYHTVGVVPVRVINPDGGTAQANFEYRNPDSKPSIIDITNQNISS
                     VSETVTIDGKEVTARIVRVNINGGNTIKVIGRDFRENAVISIGEILDIKPGQVDYSGL
                     PTSLSFEMPAVSEKNIGKYYRLVVLNEDGGTASSDELIPPIYITFIKGESNPQVISMT
                     PDSGPSTGGTNVTIEGKDFRSTMAGFSGTIQVFFGEVPATNVTVSADGRFIYLVTPPH
                     EPGAVEVKIQNPDGTMAKVPKPFTYISNPKITKITDINELTELKTISIEGGQEIKLKG
                     YGFKPGAKVYFNPVLKKAEGESSQKVFYIDGVPYILESGIEATKVEFIDGETVKVVTP
                     EGNKDSSGVILINQDGGASNVYGIKYSLPEVTPPEKVTADLIYDRYIKVTWTPVPGAI
                     EYEIYVVINDSNIEMVGTSKTTSFVYEDLEPRTSYKFVVKALGEFNISQASMESNTVR
                     TGNVAGIPDTDGGLSEKTISSKKGTIAEVFIGRNDYNKALTIDLIRGDLAGAKEIVIS
                     IPAEVVTSYYAKDITVIGRDFVLVLNPNAFKTETMLANSNNKSAGVRFRMYPATNNPN
                     LEKGQTALSTGYVLEAYQYVGKSNTAMEHLNSSILLNLDYDGNLSSLRRLNYVSFSRY
                     DYYNKQWRTIKQTHDNIQMAIGDYIDRLGQYMVIGSKR"
     gene            8971..10638
                     /locus_tag="PLG49_07160"
     CDS             8971..10638
                     /locus_tag="PLG49_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17135.1"
                     /translation="MWKKAMNKITIALLLLCMVSANTPKVFADTNIKTAEGFLLEMGE
                     KQVTVEEYDGSVRTFPFNSYYILRIDTLNAEPSDFKPGMEVYLELKNNKVTYLEGYST
                     VAPGHIPEGSKIRFGTIKKIDRDQLIVKLDNGQEETYFTNSGTILLKDGLKVPLSSLY
                     EGDKVKLYFDEIDTKMISRMAVQGKSVEVKGLYKGELALADVIKKELTLTDLKIFRNG
                     GWENVGSSKTISYDERSPIHIGGQRISPEKLKYYKDNTVYLAVKDYFSTDRVESMVLK
                     NQDESVYTDRIEDINWYGNSMELEVSHKNLTFNEGTIIIKNGRLSDISALDPGMDVFV
                     VGDDSAGSMAANIVYVYNEGINNSNIGQFYIYEGRLDAVLRNSLMLKDFAYLNNNSWE
                     AFSTSKELFYDEDTVIYDLDERRIISSEEFYYGDYAVDEDSNYARENNLEDRYVYAYT
                     DGDRVVAIMVQKNRDSLLKLRVTSGVALEAPENHKWTGWRIHIANAADWSSRKDQWMA
                     RKDSFYLTLEDAIIIKNGQIITSDQLKQNDRLYIIRDDLKAKVIIVK"
     gene            10652..12148
                     /locus_tag="PLG49_07165"
     CDS             10652..12148
                     /locus_tag="PLG49_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOQ17136.1"
                     /translation="MKKLAWTMIFILLFHSMAWAAPIEFSGGVSDEFQYKEVVFLSGK
                     PVVFVGTVNVNIDEKQEEKKATYTFTLSPEDIDIEGKLTRKVTLITTYTPVTGKGQTI
                     ANTKVTRYTENVTIGQDTFKLTNSDIQFSQSDIIDNRPAIDFYSGNIQALKRYEINGG
                     EGIAQVKITGGDVGYKNFWGSTQTQLLDYVYTINRKAQGENENSSWEGTVSVKVSDST
                     TKSLQYSQNEANFSSFNGGYIQVVKEGMISQYTYNLPRITDEGIDPFGRNQGTISLQA
                     SKVPKVERLIVPKFRDTAGHWAQQDIEKLYSLGVFEGNTSFFIPDGSMTRIDFVKAVV
                     KACDIGGNEEADSSSKRSSRKKKGEVEEIIFVDIDSSHPDYQYVKKAFEKNIITRGTD
                     YRFNPNEPLTKAEAVTILIRVLGLEGKAPAPGYKTSFSDDSKIPNWAKDAVYMAAEAG
                     IIGGDNNNNVNAHKQLKRSEASALLVRFLEFLEKDLQKDYSENIIYYY"
     gene            12358..13434
                     /locus_tag="PLG49_07170"
     CDS             12358..13434
                     /locus_tag="PLG49_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOQ17137.1"
                     /translation="MYFDDLLELQAYVKGLDIAEDEQLMVLVGDESGELVNNLITFMN
                     RSMIKFFGGIFPGLLVGTEMRKNGFILEKFKTIFSTMVIPDLAEPFQKISQEIKGSTV
                     IVLADGLSSRMKELTDIVYDNLGPEVTYFGGGAGYYDLQHRPCVFNNEGLFEDAAIVF
                     VIPNQTVFEVKHGWEQLAGPFTVTKAQHNTMKELDFQNPFELYRAIIEQEEGIILYST
                     DFFEVAKDHPFGILKTDDELIVRDPINLTDKDEIVCVADIPENSIVYVLKGDRHTLFQ
                     SSIEVVEECVKNAPNKYKPFLFDCISRAMFLQDHFKSELTNIQERLLYPLEGALSIGE
                     IASLRNGMIEIHNKSTVLGLINVI"
     gene            13560..15707
                     /locus_tag="PLG49_07175"
     CDS             13560..15707
                     /locus_tag="PLG49_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOQ17138.1"
                     /translation="MRQEKILQWIVNIGLGLILFILPFPRGLFFEKEIVPVQIAIFAI
                     FILWSFIKIKKKENLEIDSFLFIAVLLFPICYILPLIFGKAASHYGTLMYIFRYLSYV
                     AVFIMLSDITRSKRQLFVWLNILALSAVTAALLGIDAGLGGIINNFFGFDGYIDEFGR
                     ICGVLQYSNSFAAYMGMLFFILIALGGLVEKRYQKAIYAALGVLPLTVLYTTVSRGAI
                     LFVPIIYLFLILIIPTKEKRLEMVLFPLPAVVLSLFSGKELSNMISQSMNGGQVSIFK
                     GWFIVLAAIGFALLFAYVFLYFHVFLSKISTRTYQIAIAVLFVAGLAGGIAVIKTGFY
                     EKILPEFLVVRFREMTSFTELVSGRNNFYRDGLIMLKDHWFLGAGGNAWSAMYQKYQS
                     YNYGSTEAHSFPLQLWLETGLLGILTLIFLVAALIILYFKNRKSEKGISVTLTLIPVL
                     MLFTHSIIDFNFSYFSLPLICFAILGCANGIKAKKDLDFSIASWMAFLLGMVLILFPV
                     SWQIARNYAVKATDIMLQEKITGQDVLDSIGYMKKAVEYNGWNSYYVIREGEPQDKDL
                     LFDLDSLYKQIYKILERNDQEQLAGLLLEQENYYTKVYELEPYSSYIAMKQAELLLKM
                     GRIDEALEKAEESLKLNPMNPIRYQDISEIYLTVGEYYTAQGEKETAKGYLERILQVE
                     KDIEEVNTRAIEKVGMTEDTKKNIERASQLLNE"
     gene            complement(15735..16142)
                     /locus_tag="PLG49_07180"
     CDS             complement(15735..16142)
                     /locus_tag="PLG49_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17139.1"
                     /translation="MFHSNPRENKELKDRSGMDQISDENKRRIEQLENLVEAHTRTER
                     HLEQHSDIASEEQLERAKELQRIREEEIEKLENIIAYGENYDNDKLKNVQKNYEYTQG
                     YINHNIDHMDEETLKKTLEKQEHRKEQIEFLKD"
BASE COUNT         5557 a         2314 c         3278 g         5003 t
ORIGIN      
        1 cagtaagcaa tggatttacc aaattttgca cggcatttaa tattgcaatt tataacccca
       61 aataaaaggg gtttttataa aggcaaatac gaattatgtc gatattatta tataaaatag
      121 ggctttttta gatttaaata aatggtaaag atggaatgat taaagcttta taatttgatt
      181 ttgggatgaa aatgaggagg gattatattg ggtttttata tagagcgtaa aaggggactg
      241 tttttgattt ttattttatt attaatagtt tgtataattc ctcagaactt acaggcagca
      301 gaaaagaaaa ttgaagcttt agaaaacaat ggggcaaagg ttacagacat ctggcaaaaa
      361 gggcaatgga gttttacaat aagaggaacg gggcttacgg ataatattaa agaaatccgt
      421 ttaataagag tcaatgatgc cctggttatt actatagata aaaaggatat tcagaaaaaa
      481 aatgatcagg aattggtcgt taatataact ggtgaaaatg ccaaaaaatt ttcatcggaa
      541 caatatacag gtttatacga tatagaaata gaatatcttg acggaagtgt cttatcgccg
      601 gcagaaagtt ataattccat aaaaatgact gtaaaaggac aaaattttaa agaggatata
      661 gagtttgttg aattgaaaaa cttaaaaacg ggtgcatggg aaattatttt agccaaaaat
      721 aaagttcagt ataaagatga aaataccctt attttgactt tggacaaaga tgaagtcaaa
      781 aaacttgcag gtggaattaa tgcttccgat attgaaatga ccgtatatta tggtaaagac
      841 tatatttttg ataataagca aagctatttt gatatttatc ttttaggaga aaattttaac
      901 aggggcgttg aaaaagtcat cctgcgtcct attgcaggag aagccttatt gcttcctgtt
      961 gatgagcaag atattatcat ggaaaaagaa gatatatcgg tagtaaatgg ctcaaaacta
     1021 agagtcagga taagaagaga taatttagat aagattaaag aattcaggca ttcaggtgat
     1081 tataaatttg ttgtggttta tactgcaggg acaggtctta aagattatac tgcatcaatg
     1141 gatttaaaac ttgtttacaa ttatggttta caaacaaaag agtccgtagt ttatagagat
     1201 aatttggtat ttgataataa ggattatatt acagaccctt tcagatttgt tttactgtct
     1261 aaatcttcac caaaggttat tgaagtatat cctcaatctg tgggaggtta tccctggttt
     1321 aatgaaaaga atttatctca tgagatctta aaggacagga attttttaaa gattactttt
     1381 gaagatattg atgggaaact ggaatttaat ccatatctgg ggattgataa tttactgggc
     1441 agcagtgtga tggcaattgg aagcaattca gactttttag accgtgattt tattatactt
     1501 tgcagggata atcctgaaat gattcataca tatctgttta aacaagacag gaccaatcaa
     1561 aaagcttatt tatatattcc agtaaaacca cttgttcccc aaacccaata tgtggttaat
     1621 atttctggga acattgtgag aaatgacagt tcggataacg ggatgattga tgaagggcaa
     1681 agatacaata agccaatatc atgggaattt tctactatgg cttccccttc cgtatcagaa
     1741 gaaggggttc tggtacaaac cgttattgag ggctatgcat cttccagtta tataaaaata
     1801 tatggtgacg atttttataa atcttctgta agagtctttt ttaacgatac acaagcagac
     1861 caggtacttg tcgaacaaga taataacgga aaatcttatc taaaagtata tctgccaacg
     1921 ggtagcagaa ggctgaaagc aggcttatac aatattatta ttaaaaatga cagtgaccat
     1981 gaaacggtta tttatggagg tcttagtgtt gttgagaaag gcgatgagat tccgacagaa
     2041 ggttatcatc ttaaaacaag aaccgtcgaa ggagaagttt tatctgatgc gtatgtgagt
     2101 catgatactt tatatttaag gtcaaaatac agcgattatt atcttttaga attgaacctg
     2161 gataagttaa tgggagaaga agtactcaat aaaacagtaa attatatagg caacagaaat
     2221 gattatatta atcgcttatt gcttaaaaca aaagatgtag atgctacttt ttattatttg
     2281 accttaaaag aaaatgcttc atccaaagaa atcaatatca atattgggag ggcgaagcct
     2341 ttagttgtac aagccattaa agacaaactc aaagataaaa aaattaaatc cgagtttatt
     2401 gaaatttcta caagtaattg caattgggat aatctttata tttccattcc atataagaac
     2461 agcagtggag aaaatctttc catataccgt tatgatgaaa tcagaagaac ctggacgaaa
     2521 gaagacagtt ataccgattt aatcaatcaa aagataaggg cattcgttaa gaaggcggga
     2581 atttatgttg tgatagaaga ctaaaaggca agaagaaatt gagtctggga gttgagaata
     2641 tgagaaaaaa gcttaaaaga tatctggcgt ttttaatggt tttgattttt aatatttctc
     2701 ttttaccatc agattttgta tatgctttcg atatgaataa ctttagtatt acgagtatta
     2761 cgattggtaa aacttatgac agaaaccgta tctttaacaa tgcctatatt accattacag
     2821 gtaagcattt gaaagatgca agtgttggga ttattaccta tggggaaggt tatattcctt
     2881 taggtacacc taaagtaaat tccgatggga ttttgcagtt tgaaatatct gaaaaccaat
     2941 taggtgaatt tctttctatt gaaggtatta cgattcctat taatgaagcc aatatgccca
     3001 atttaacgga tatcagcagg aatgtgaaaa taggaacaga tgatttagtg attaggggaa
     3061 cccgtttaaa taatgtggat ggcacgaata ttgttgcccg ctatggaacg ggaaactctt
     3121 atacgacact tggggctttt agtaatgcca cggaagtgac tatttcaaaa ccttcaggtt
     3181 cattaggtct tcacgaattc attttttcaa gcagtnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnntattt caaaaccttc aggttcatta ggtcttcacg aattcatttt
     3361 ttcaagcagt tttaaggtat atgacatcga ttttaatgat aataatcaaa agaaaagcgt
     3421 agatattcaa attaattata cttacaagga tcaattcaga tttataacgg atttggctat
     3481 tgaaggcgat ctgaatatgt ttcccaacag gggggaagca ggagatattg tttattttac
     3541 agcagataaa ttagatgaat acgatgtctt tttcttaaaa gatttatctg gaacagattt
     3601 atattcaaat gccaataaag gggttaatac tacttataag gcaaagagtt cttccgatga
     3661 aaaggatact ttaacaaccc aggtacccaa acttcctgta ggggaatatt atgttgtttt
     3721 aaccaataaa atttcttcgg gtaaagaccc tatgaaagaa gttaccgggg aaagagtttt
     3781 agctgagaaa tttaccatta ttgacggaag taataaagca gttatcgtgg atgttcagcc
     3841 taataaaggt cctgatacag gggaattagt aactatttca ggtcaatttt tgggcagcct
     3901 gaatatatct gaatttaccc cttcacagga ttctaccctt acggtgaatc aaaattcatc
     3961 tccagaggaa ttggagatcg attatggaca gggtacatat ggtccatcgc agataaagat
     4021 agtatctgcg aaaagaatta tccgggtaat tatcggaaat aaagcagctt ttgttaaaga
     4081 taatggtgaa tatcaggttt tctttaataa agatttagac aagattatcg ttagaacagc
     4141 tcaagtaacg gatgcagata taaatccggt aagagatgtt gtcattgaaa cagaaacagt
     4201 ccttacaaag gaagacggca gtaaaattgt ttttaaggaa agagcggtta aagaaaaggg
     4261 ctataccttt atagtaagta agattaaacc tgaaattacc gaagcagtgc ccaacaaaat
     4321 acaagtcgtt aataatggtg ggacatttca aattccttcg gacaggtata ttgcgattta
     4381 tggtaaaaat ttcatgattc ataaatacac caatgaagac ggcagggaag ttataagata
     4441 tcctattgtg caattgggac ccaatctgat tattgataag aacaatcctg cttcggactt
     4501 taatgatccg ggtaagagcc ttaaaggtaa agatgaagtt taccttaagg tatttgatgc
     4561 ttacggaaga gaactggatg gttccagtgg taacgaaatg ggaacaaaga tattaattaa
     4621 gttagaagaa ggcagccctg cggcaggggt tgttttaggt aaaaccttta ttaaagtaat
     4681 caatcccatg agaaattcca gggaaactgg tttagagaca cagatgaatg attttattga
     4741 atttgtaaat ccggagactt ccaaaaatcc aatcattcaa agtgtaaatc ctaatgtcgt
     4801 atcggttgaa ggtggagaag aggtcatcgt tacgggaagc aactttacag atggggttaa
     4861 ggtttttatt gatggaaaag aagtgactcc aattaacagg ggtgctgatg gtaaaaccat
     4921 tacttttaaa gcacctgcgg gcagggaagg acaaacccaa ctccaagtga tgaatataga
     4981 aggtggaatg gatgtttacc ccttcagtta tgtaaaaacc tttaccaatc ccaagattac
     5041 atcgattgaa cctcgaaaag gaaatacagg aactttagtc gttgtaaaag gggaaaactt
     5101 tttaaaacct gatcctacag gcggagaaag tgccatttac cgcttaatag gaaccaggat
     5161 attgctagaa ggagaagata ttaattctta taatatcaat acagcaacta aagaaattga
     5221 gcttgtggct tttacaccca atacacctct tatttctgtg caaaacagca ggatacaagt
     5281 agcaagttat tatcatagta ttttgctgcg ggcgccaggg ggaaagcttt ataccttaga
     5341 tgtcgattat tcaggtgtgc cggttatttc tgatggtatc ggcaacgatt ataaaatcgt
     5401 ctataatgtt cagtcaggta aattacaagc ggacaaagct ggcggaggct tatatgattt
     5461 aacggtttcc ggaggaaata ttaccataca gcaagattct ggtcttagcc ttcaaattgt
     5521 aacgcctttt gaagtaaaag acggagaagt gattggtgat ttagttaaag tggttgataa
     5581 aaataccatt tactttacag tgcctatttt acctgctgac ggatggtacg atgtgacggt
     5641 tcaaaaccct gataccaaaa aagataccaa aaaagataaa gatggatttt attattacaa
     5701 gcaacctcaa agtactcctg ttatcgatga agtggttcct tctgaaggga gtgttgcagg
     5761 aggcaatgtg gtaaccatta aaggtaaaga gtttatggat aatggctttg aaaaagtaag
     5821 agtatttatt aacggggtag aagtagcccc agccaatatt caggtttcta ctacaagcga
     5881 cagcataacg gttgtagttc ctccttatag tggggattta atggcggaaa aaggtgtagg
     5941 cagattatct gttcctgtgg ttgttatcaa tcctgatggg ggaagtgctt caaaagaaaa
     6001 cgcttatact tacgttattc caagcagtaa tccaaaaata gaaagaattt taccaacgtc
     6061 agggactgct gccggtggtg cagttgtaga aatttttgga agtgatttca ggacttttga
     6121 gccttatgaa gattataacc gaaaccaaat cagagatgag aatgaaccat acagggattt
     6181 aaacaataat ggtcagcatg atatgacttt tgatcctgaa aaggacaaaa aggaattaga
     6241 tcatcctttg tatagttatt attatgattc tcctcttttg cccaaaatat attttggcat
     6301 ggaacaagct ttaattgtag aattttcagc tacatatatt aaagtggtaa ctcccagggg
     6361 aagtggaacg gtaccagtct ttatagaaaa taacgattca ggaagatcca ataccgttta
     6421 ctatagttac cagggttcca aacccaaaat tacgaaggta tcccctggtg taggaaacag
     6481 aatgggaaat actaatattg gcatctatgg ggaagatttt agacaatcca atattaaagt
     6541 ttataagtct gtcagcgaaa aagaaacaag aaccatgact ctggtacgat ttggagataa
     6601 tacgaataaa aacatcccaa gagatcaggc taattccgga ctgattaacc agggaaatgc
     6661 ccaggtatct ttagagaaag attttacagc catatataaa gcagagtatg acgataccta
     6721 tgacagtttt tctgaaatca gggttgtcat taaatataat gatgtcattt attctaatac
     6781 gtttaaacgt tttaatgatg aagaatcttt tattaatacc aatttattag ttgacagcga
     6841 tggaaacaga tatccattta gtgaactgat ccgttttgct gttgaagaca gaaggtttat
     6901 tgtagaaaga ggttatgctc ctcaagtgat atatatttca agtggggaac tttctgtaac
     6961 aataccgcct tatcataccg ttggggttgt tcctgtaagg gttatcaatc cagatggcgg
     7021 aacagcacag gctaattttg aatatagaaa tccggatagt aaaccaagca tcattgatat
     7081 tacaaatcaa aatatttctt ctgtatccga aaccgttacc attgatggca aggaagtaac
     7141 ggcaagaata gtaagagtca atattaatgg tggcaatacc atcaaagtga ttggcaggga
     7201 ctttagagaa aatgccgtaa tttctattgg ggaaatttta gacatcaagc ctggtcaagt
     7261 ggattattca ggccttccta ccagtttaag ctttgaaatg cctgctgtat ctgaaaaaaa
     7321 tattggaaaa tattatcgtt tagtggtatt aaatgaagat ggaggtacag cttcttctga
     7381 tgaattaatt cctccgattt atattacctt tatcaagggt gaaagtaatc ctcaggtaat
     7441 cagtatgaca cctgacagtg gtccatcaac gggtggaacc aatgtgacca ttgagggtaa
     7501 agacttcaga agcactatgg caggtttttc aggtacaata caagtctttt ttggagaagt
     7561 acctgctacg aatgtaaccg tttcagcaga tggaagattt atctatttag taacgcctcc
     7621 tcacgaacca ggagctgtag aagtaaagat tcaaaaccct gatggaacaa tggcaaaggt
     7681 gcctaagcca tttacctata ttagtaatcc taaaattact aaaataacgg atattaatga
     7741 attaaccgaa ttaaagacca tatccattga aggtggacaa gaaatcaaat taaaaggtta
     7801 tggatttaaa ccaggcgcca aagtatactt taatcctgtg cttaagaaag cagaaggaga
     7861 aagcagtcaa aaggttttct atattgatgg tgtaccttat attcttgaat caggtataga
     7921 agccacaaaa gtggaattta tagatgggga aacggttaag gtggtaaccc ctgaaggtaa
     7981 taaggatagt tcaggcgtta tattaattaa ccaggatgga ggagcaagca atgtttacgg
     8041 cataaaatat tctcttccgg aagttactcc tccggaaaaa gtgacagctg atttaattta
     8101 tgacaggtat ataaaagtaa catggactcc tgttcctggt gccatagaat acgaaattta
     8161 tgtggtcatt aatgacagca atattgaaat ggtaggaaca agtaaaacca ccagttttgt
     8221 atacgaagat ttagaaccaa gaaccagtta taaattcgtt gtaaaagcgc tgggagaatt
     8281 taatatttca caagcttcaa tggaaagtaa tactgtaaga acaggaaatg tcgcagggat
     8341 accagataca gatggtggat taagtgaaaa aaccataagc agtaaaaagg gtactattgc
     8401 agaagtgttt ataggcagaa atgattacaa taaggcattg accattgatt taatcagagg
     8461 ggatttagca ggagcaaaag aaatcgttat cagtatacct gcagaagtgg ttacttctta
     8521 ttatgcaaaa gatattacag ttataggcag ggactttgtt ttagttctca atcccaatgc
     8581 ttttaaaaca gaaacaatgc ttgccaacag caataataaa tctgcaggtg taagattcag
     8641 aatgtatccg gctacgaata atccaaatct agaaaaagga caaactgctc tttcgaccgg
     8701 ctatgtcctg gaagcttatc agtacgttgg aaaaagtaat acagctatgg aacatttaaa
     8761 ttcaagtatt ttattaaatc ttgattatga tggtaatctt tcttctttaa gaaggttgaa
     8821 ttacgtttcc tttagcaggt atgattatta taataaacaa tggcgaacca tcaagcaaac
     8881 ccatgacaat atacaaatgg caataggaga ttatattgac agactaggcc aatacatggt
     8941 tattggtagc aaaagatagg ggtgaaggcg atgtggaaaa aagcaatgaa taaaataaca
     9001 atcgctttgt tgcttttatg tatggtatct gcaaatacgc ccaaagtatt tgcagatacc
     9061 aatataaaga cagcagaagg ttttttactt gaaatgggag aaaaacaggt aactgtagaa
     9121 gaatatgacg gaagcgtccg tacttttcct ttcaacagct attatatatt aaggattgat
     9181 acgcttaatg cagagcccag tgactttaaa cctgggatgg aagtctattt agagttgaaa
     9241 aacaataagg tcacttattt agaaggttat tccacagttg ctccgggtca tattccagag
     9301 ggcagtaaaa ttaggtttgg aaccatcaaa aaaattgaca gggaccaact gattgtcaaa
     9361 ttggataatg gtcaggaaga aacttatttt acaaactctg ggacaatttt attaaaagac
     9421 ggacttaaag tacccctatc cagcctttat gaaggggata aagtaaaact ttattttgat
     9481 gaaatcgata cgaaaatgat aagcagaatg gctgttcaag gtaaatctgt tgaagttaaa
     9541 ggtctatata aaggggaatt ggccctggca gatgtcatca agaaagaatt aactttaaca
     9601 gacttaaaaa tttttagaaa cggaggctgg gaaaacgtag gcagttcaaa aactatttct
     9661 tatgatgagc gttcaccgat tcatattggt ggtcaaagga tatctcctga gaaattaaag
     9721 tattacaaag ataatacggt gtatttagcc gttaaggatt attttagcac tgacagagta
     9781 gaaagtatgg tgttaaaaaa tcaagatgaa agtgtttata ccgacaggat agaagatatc
     9841 aactggtatg gtaattcgat ggaactagaa gtcagccata agaatttaac ttttaatgaa
     9901 ggtacgatta ttatcaaaaa cggacgtctg tcggatatca gtgctttaga tccaggcatg
     9961 gatgtctttg ttgtaggcga cgattcagct ggttcaatgg ctgctaatat tgtttatgta
    10021 tataatgaag gcattaataa ttctaacata ggtcaattct atatttatga aggcaggttg
    10081 gatgctgtat taagaaacag cctcatgcta aaagactttg catatttgaa taataacagt
    10141 tgggaagcct ttagtacgag caaggaattg ttctatgatg aagatacggt gatttacgat
    10201 ttagacgaaa gaagaatcat ttcatctgaa gaattctatt atggggatta tgccgttgat
    10261 gaggacagca attatgcaag agaaaacaat ttggaagacc gatatgttta tgcttatact
    10321 gatggcgaca gagtagtggc aattatggtg caaaaaaaca gagattctct cttaaaatta
    10381 agagttacca gcggcgttgc cctggaagca ccagaaaatc acaaatggac aggatggaga
    10441 attcatatag ccaatgcagc cgactggagt tcaagaaaag atcagtggat ggccagaaaa
    10501 gattccttct atttaacttt ggaagatgct ataattatta aaaatggtca aatcattacc
    10561 agcgatcagt taaagcaaaa cgacagatta tatataatac gtgatgacct gaaggccaaa
    10621 gttatcatag taaaatagag aggaagatta gatgaaaaag cttgcctgga caatgatttt
    10681 tatactgttg tttcatagta tggcctgggc tgcgccgata gaattttccg gtggcgtatc
    10741 cgatgagttt caatacaagg aagttgtatt cttatcagga aaacctgttg tctttgttgg
    10801 aacggttaat gtaaatattg atgaaaagca agaagagaaa aaggctactt atacttttac
    10861 tttaagccct gaagatatag atatagaagg taaattaact agaaaggtaa ctttaattac
    10921 tacttatacc cctgtaacgg gtaaggggca aaccatagcc aatacaaagg taacaagata
    10981 tacagaaaat gtaaccattg gtcaggatac ttttaaatta acgaacagtg atattcaatt
    11041 ttctcagtca gatattattg acaaccgccc tgctatagat ttttattcgg gcaatataca
    11101 agctttgaaa cgttatgaaa ttaacggggg agaaggtatt gcccaggtta aaattactgg
    11161 cggggatgtc ggctataaaa acttttgggg cagtacccag acgcagcttc tcgattacgt
    11221 ttatacaatt aacagaaaag cccagggaga aaatgaaaat tcatcctggg aaggaactgt
    11281 aagtgtaaag gtttctgaca gtacaacaaa gtccctgcag tattctcaaa acgaagccaa
    11341 tttctcaagc tttaacgggg gctatattca agttgttaaa gaagggatga tatcccaata
    11401 cacatacaat cttcccagaa taaccgatga aggcatcgat ccttttggaa gaaatcaagg
    11461 aaccatttct cttcaagctt caaaggtacc taaagttgaa agattaattg ttcccaaatt
    11521 cagggatacg gctggtcact gggcacagca agacatagaa aaactatatt ctcttggcgt
    11581 ttttgaaggg aatacatcct tctttattcc cgatggcagt atgacaagaa ttgattttgt
    11641 aaaagcagtg gttaaggctt gtgatatcgg aggaaatgaa gaagccgaca gttcaagtaa
    11701 aagaagcagc agaaagaaaa aaggagaagt agaagaaatt atatttgtag acattgattc
    11761 ttcccatccg gattatcaat atgttaaaaa agcttttgag aaaaatatta tcacaagagg
    11821 cacagattat cgttttaatc ccaatgaacc tttgaccaaa gcagaggcgg ttactattct
    11881 tatccgggtt ttaggcttag aaggtaaagc acctgctcca ggatataaaa cttctttttc
    11941 ggatgacagt aaaattccta attgggcaaa agatgccgtt tacatggcgg cagaagcagg
    12001 aattataggc ggagataata ataataatgt taatgcccat aagcagttaa aaagatcaga
    12061 agcttctgcc ctattggtga gatttttaga attcctggaa aaagatttgc aaaaagacta
    12121 tagtgaaaat atcatatatt actattagta aataagtcct ttaacttaaa ggcttcctga
    12181 agaatgcagg aagccttttt taaaatggat ccttgaaaat tttaattaat cataagtgtt
    12241 tgaatttcta taaattaggt aattttactt attatatagt attattgaaa aaatttttaa
    12301 ttatgatata ataaaataag gtggttaatg atatatagct aaatgggagg tgtaaccatg
    12361 tattttgacg atttactaga acttcaagct tacgttaaag gcctggatat tgctgaagat
    12421 gaacagctta tggtactagt tggtgatgaa tcaggagaat tggtaaacaa cttaattacc
    12481 tttatgaacc gcagcatgat aaaatttttt ggaggaattt ttccaggttt attggttgga
    12541 acagaaatgc gtaaaaatgg ttttatcctg gaaaaattta aaacaatttt ttcaacgatg
    12601 gttatacctg atctggccga accttttcag aagatatcac aagagataaa aggttcgacg
    12661 gtaattgtct tagcggatgg tttatccagt agaatgaaag aactgacaga tatcgtttat
    12721 gataacttag gtccggaggt tacatatttt ggtggtggag caggttatta tgatcttcaa
    12781 caccgtccct gtgtatttaa caatgaaggt ttgttcgaag atgcagcgat tgtatttgtc
    12841 attccaaatc aaactgtttt tgaagttaaa catggttggg aacaattagc cggacctttt
    12901 accgttacaa aagctcagca taacacaatg aaagaattag atttccaaaa cccgtttgaa
    12961 ttatacaggg cgattatcga acaagaagag ggtattattt tatatagtac agatttcttt
    13021 gaagttgcca aagaccatcc ttttggaatt cttaaaaccg atgatgagtt aattgtcaga
    13081 gatccgatta atttaacgga taaggatgaa attgtttgtg ttgcagatat tcctgaaaac
    13141 agcattgtgt atgttttaaa aggagacagg catactttat ttcaatcatc tatagaggtt
    13201 gtagaagaat gtgttaagaa tgctccaaat aagtacaagc catttctttt tgactgtatt
    13261 tccagggcaa tgtttttaca agaccacttt aagagtgagt taacgaatat tcaggaaaga
    13321 cttctgtatc cgcttgaagg agcactttct ataggtgaaa ttgcttctct tagaaatggt
    13381 atgatagaaa tacataataa atctaccgtt ttaggattga ttaatgttat ttagggggga
    13441 tgtccccctt ttttagttaa attgaaaaga aaaacagtta aattttaata taaggttttt
    13501 tgcagataaa ttgtatgata taataataaa gtaaatttta cgagtacatg gaggagcaaa
    13561 tgagacagga aaaaattctt caatggatag taaacatagg cttaggatta attttattta
    13621 ttcttccttt tccaagaggt cttttttttg agaaggaaat cgttccagtt caaattgcaa
    13681 tatttgcaat ttttatttta tggtctttta taaaaataaa aaaaaaggaa aacttggaaa
    13741 tagatagctt tttatttatt gcagttttat tatttccgat ttgttatatc ctgcctttga
    13801 tttttgggaa agcagccagt cattacggga ctttaatgta tatcttcagg tatttatctt
    13861 atgtggctgt ctttatcatg ttatcagata taacaagaag taaaagacaa ctttttgttt
    13921 ggcttaatat attggcttta agtgctgtca cagcggcctt attagggatt gatgcaggtc
    13981 ttggaggaat tattaataat ttttttggtt ttgacggata tattgatgaa tttggtcgta
    14041 tatgcggggt gcttcaatat tccaatagtt ttgcagcata tatgggaatg cttttcttta
    14101 tactgattgc tcttggaggt ctggttgaaa aaagatatca aaaagcaatt tatgcagcat
    14161 taggcgtact gcctcttaca gtcttgtata ctactgtatc aagaggagct atcctttttg
    14221 ttcctatcat ttatcttttt ttaattctta tcattccaac taaagaaaaa cgtttagaaa
    14281 tggttctttt tcctttacca gctgtagttt tatccttatt ttcggggaaa gagctaagta
    14341 atatgatttc tcaaagcatg aatggaggac aagtttcaat attcaaggga tggtttattg
    14401 ttttagcagc tattggattt gccttattat ttgcatatgt attcttatat tttcatgttt
    14461 ttctttctaa aatttcaacc aggacctatc aaatagcaat tgcagtgtta tttgttgcag
    14521 gacttgctgg aggaattgca gttattaaga cagggtttta tgaaaagatt ttgccagaat
    14581 tcttagtggt tcgttttaga gaaatgactt cttttacgga acttgtttca ggcaggaata
    14641 atttctatag agatggacta attatgctaa aagaccattg gtttttggga gctgggggca
    14701 atgcatggtc tgctatgtac caaaagtatc aatcttataa ttatggctct acggaagcac
    14761 acagttttcc tttacagcta tggcttgaaa caggtttgtt ggggatttta actttgatat
    14821 ttttagtagc agctttaatt attttatatt ttaaaaacag aaaatctgaa aaaggaattt
    14881 cagtaacatt aactttaata cctgttttaa tgttgtttac ccatagtatt attgacttta
    14941 atttttcata tttttctctt ccgttgattt gttttgcaat tttaggttgc gctaatggca
    15001 taaaggcgaa aaaggacctg gatttttcaa tagcttcctg gatggctttt cttttaggaa
    15061 tggttttaat cctgtttcct gtatcatggc aaatcgcaag aaattatgcg gttaaggcta
    15121 cggatattat gcttcaggaa aaaataaccg ggcaggatgt tttagattct attggttata
    15181 tgaagaaggc agttgaatat aatggttgga attcttatta tgttatccgg gaaggggaac
    15241 cgcaagataa agatttattg tttgatttag atagcttata taagcaaatt tataaaatac
    15301 tcgaaagaaa tgatcaggag caactggcag gattattact ggaacaagaa aattattaca
    15361 caaaagtgta tgaattagaa ccctatagtt cttatattgc catgaaacag gcagaattat
    15421 tattaaaaat gggcagaatt gacgaagctt tagaaaaggc agaagaatct ttaaaactta
    15481 atcctatgaa tcccataaga tatcaggata tatccgaaat atatcttaca gtgggagaat
    15541 attatacagc acaaggagaa aaagaaactg caaaaggata tttggaaaga attcttcaag
    15601 ttgaaaaaga tatagaagaa gttaatacaa gagctattga aaaagtggga atgacagaag
    15661 atacaaagaa gaatatcgaa cgtgcaagtc aattgcttaa tgaataaagg ccagagaatc
    15721 tggccttcta ttttctaatc ttttaaaaat tcaatttgtt cctttctgtg ttcttgtttt
    15781 tctaaagttt tcttaagggt ttcttcatcc atgtggtcta tattatgatt aatataccct
    15841 tgggtgtatt cataattttt ttgcacattt ttaagtttgt cattatcata gttttctcca
    15901 taggctataa tgttttcaag tttttcgatt tcttcttccc ggattctttg caattctttt
    15961 gctctttcca actgctcctc ggatgcaata tctgagtgtt gttcaagatg gcgttctgtt
    16021 ctggtatgag cttcaaccag gttttctaac tgttcaattc ttcttttatt ttcatctgaa
    16081 atttgatcca taccacttcg atcttttaat tccttatttt ctcttggatt gctgtgaaac
    16141 ataataagcc tcctttatga taaactagtt atattttttc acaaagcatc aaaaataata
    16201 ccatgaaagt ctggaatata tttttaaaaa gaagaataca aagtcttagg gc
//
LOCUS       DAOSUD010000048        16132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2224_length_16132_cov_25.026746, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000048 DAOSUD010000000
VERSION     DAOSUD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_16132_cov_25.026746"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLG49_07185"
     CDS             <1..345
                     /locus_tag="PLG49_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17140.1"
                     /translation="ANSVCEAAEEGDAEIAVILPANSCITEIFVSGRSADDFDSILIT
                     VNNGTENIFCQRFYVAEDAGVAGVNFETALTRPLCVGAGGATVTATIEGGTADEGLLT
                     VVYCESCCSAEE"
     gene            complement(380..847)
                     /locus_tag="PLG49_07190"
     CDS             complement(380..847)
                     /locus_tag="PLG49_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17141.1"
                     /translation="MANPLKIRLGKLIQFIFDILRPNTSNSTDPRVPVILPPASEPVT
                     LSCITVPVSKESPLVWLSATIGSLSVFSLDPSRLVFSIWRGQPDTGELIFSTADNGQG
                     NGTSRTTTFTHVDQPNRRFCRKITYCLTAQAFDNEDNINIIGPVTFTGASIKN"
     gene            complement(867..1352)
                     /locus_tag="PLG49_07195"
     CDS             complement(867..1352)
                     /locus_tag="PLG49_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17142.1"
                     /translation="MSSSFQINIERLSNFAYDTLNPNISDSTDPRVPIPLDNTTGEVI
                     LSTIRLPVQKEKSFIWLSATVGFRVNGPDASNPPAAMIFRIYRGNPDTGNLIYTTTDS
                     GQGGAGEPTFRTTNLNHVDTQKKTCSCVSYTLTVEAVDIESIDIIGPITFTAAEILDS
                     N"
     gene            complement(1383..1856)
                     /locus_tag="PLG49_07200"
     CDS             complement(1383..1856)
                     /locus_tag="PLG49_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17143.1"
                     /translation="MNTLQPFQITNGIIRDFSFDVLNPNITNSTDSRVPIVITAADPP
                     VILAQITIDIPEKKNAVWLFASLGFSARSDLTGPAAIFRIWRGHPSNDFEIFSTADNG
                     QGSITVGTFRNSSFTTVDQLINVKCSRITYYLTVESNVATQLTFNGPISFIAVSI"
     gene            complement(1995..2738)
                     /locus_tag="PLG49_07205"
     CDS             complement(1995..2738)
                     /locus_tag="PLG49_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HOQ17144.1"
                     /translation="MEKLNQMKINRLITLFMIFILNNSLIYGEIKEAEWSMERYKDSR
                     LAIVNENEINIWQFPDTDSKVLAKANQLDVILVTGRYQDWYRIHYKYQTGWIFSKYVE
                     GFNLHLLPEITPVSSEDLFRRQIVDYAKKFIGTPYKYGGTSLETGVDCSGFTQAVMKE
                     FDISLKRSSKEQAMNGIPVKREELSPADLVFFDTSGTNNGKVSHVGLYIGNNRFIHAT
                     SSKGVKIDNLSQPYYNRAFVKAISVIGQK"
     gene            3005..4729
                     /gene="iorA"
                     /locus_tag="PLG49_07210"
     CDS             3005..4729
                     /gene="iorA"
                     /locus_tag="PLG49_07210"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOQ17145.1"
                     /translation="MKKLILGNEGVARGAYEAGVRVASAYPGTPSTEITEFIAKYDEI
                     YAEWSPNEKVAMEVAIGASLAGARAMVAMKHVGLNVAADPLFTVSYTGVNGGLVILVA
                     DDPGMHSSQNEQDSRCYARAAHIPMLEPADSQEAKDFVKLAFHLSEKYDTPVFVRMTT
                     RVSHAQSIVEFGEREEIALKPYDKNIQKNVMMPGMAIKRHIVVEERMKQLAEDANQLE
                     INKIEWNDKKIGIITSGIAYQYVKEAIPNASVLKLGMVHPLPKKLIEEFANQIETLYI
                     VEELEPVIEEQVKSYGIAAIGKEVFSVQGELSVKKILSAIAKTEIPSKKPEQLPGRPP
                     VLCPGCPHRGIYYVMKKLKLHATGDIGCYTLGAMEPLSSIDSCVCMGASVGMVHGIEK
                     ARGREFSKNWVGVIGDSTFIHSGITGLIDIVYNKGMSTVLILDNSTTGMTGHQDHPAT
                     GKTIKGEAAPILNLVELVKAIGIKNVRVVNPFDLKEAEVVLKEETNKDEPSVIIASRP
                     CALLTKEKKAACKITDACVMCGSCLRLGCPAIEKQDGKVVIDDALCNGCGLCPKVCRV
                     NAIVKDGE"
     gene            4731..5312
                     /locus_tag="PLG49_07215"
     CDS             4731..5312
                     /locus_tag="PLG49_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOQ17146.1"
                     /translation="METKNILIVGVGGQGTLLTSRIIGDVAVRKGYDVKMSEVHGMAQ
                     RGGSVVTHVRYGKKVYSPLVEEGTCDILLAFEKLEALRWIHFLKEDGIIIVNNQEINP
                     MPVITGAAQYPTDIIQRIQRACKQAYILDALSIAKALGNIKVVNTVLLGVLAKHLGLE
                     KSDWEEAIERTVPPKTVELNKKAFLTGYESEIK"
     gene            complement(5352..6275)
                     /locus_tag="PLG49_07220"
     CDS             complement(5352..6275)
                     /locus_tag="PLG49_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOQ17147.1"
                     /translation="MIFITKYRLPIAVLGLSAILIYGSVSGSFNADSILKEFPVEIII
                     LIFVLSLFTKTFENLGVFDYIKKWLIETSRGKKVLALSILMFTIYFLSLFMNNLTVIL
                     LFAFICIELAIHFKTSILPVLVSAIIASNIGGAALPWADTPAVILTLYTDFTLFDFLT
                     KLFLPCFFYIFLLILYTYFFTKKIYNPIHQPITRHNKIVVKSIPPPAPHIKKHPPLHH
                     KDEHLPPHKKEKLHHLADKNSNIPTVGSPSNSSFLFQVQKKAINIKLIMPVLLRMPLS
                     KPSFTMMKTAGYLGLRTNPLLKDLQKKIPLK"
     gene            complement(6417..6842)
                     /locus_tag="PLG49_07225"
     CDS             complement(6417..6842)
                     /locus_tag="PLG49_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ17148.1"
                     /translation="MILQIISAFFATFFFAILFNVSKKELGYCGLVGSLGWLIYLFIF
                     KWTDSVIFSNFISALSISIISRILAKLRKMPITIFLIPGIIPLVPGAGMYKTMYAVLN
                     QNFKEAAFYGAQTFEIAGAIAIAIIFISSFDTLSHKKTK"
     gene            complement(6874..7629)
                     /locus_tag="PLG49_07230"
     CDS             complement(6874..7629)
                     /locus_tag="PLG49_07230"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ17149.1"
                     /translation="MDHKLLLNFAMQAGEIMLRSGAETYRVEDTIMRILSVYDFESIE
                     VFVTPTGIIATIDDKNIEMTTKLKRINSRTIRLDKVALVNDLSRKYVLGKISLQDAIE
                     ELKKIDKISPYQPLILISVTGLTSAFFTIVFGGNYKDFLISLSIGIILGIIQRLFINL
                     QLSRFLADMLGGFLVGFLALLLTSIIPSTNFDNIIIGSIMPMVPGVAITNAIRDTIEG
                     NLLSGISRGVEAFFVAISIATGVAIALKVWLPF"
     gene            7945..9180
                     /gene="glyA"
                     /locus_tag="PLG49_07235"
     CDS             7945..9180
                     /gene="glyA"
                     /locus_tag="PLG49_07235"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOQ17150.1"
                     /translation="MYSFEEIQKVDLEVAEAIEKEINRQNNNIELIASENFVSKAVMA
                     AMGSPLTNKYAEGYPGKRYYGGCEFVDVVENLAIERAKKLFGAEHVNIQAHSGAQANM
                     AVFFAVLNPGDTVMGMNLSHGGHLTHGSPVNMSGKQYNIIPYGVDEKTGYIDYDALME
                     LAKEHKPKLIIAGASAYPRIIDFEKFRAIADAVGAYLMVDMAHIAGLVAAGLHPNPVP
                     YADFVTTTTHKTLRGPRGGMILCKEEFAKIIDKAIFPGTQGGPLMHVIAAKAVSLKEA
                     ASDEFKAYQKQIIKNAKALAEALMAKGFNLVSGGTDNHLMMIDLRNLNITGKEAETRL
                     DEIGVTCNKNTIPYDPQSPFVTSGIRLGTPAVTTRGMKEEDMQVIADIIHMTLTDFES
                     NKEKARELVAALVSKYPLY"
     gene            9243..9665
                     /locus_tag="PLG49_07240"
     CDS             9243..9665
                     /locus_tag="PLG49_07240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOQ17151.1"
                     /translation="MYIHETKIKVRYAETDQMGIVHHSNYYIWFELARTEFFEAIALP
                     YDLIERDGIMFPLVESSCIYKESARYAENLIIKAWIEEIKGMKFTFLYQVIREEDKRV
                     LAIGKTIHVPVDKNMKLINLKKKNPRLLETIKAVISAK"
     gene            9774..10496
                     /locus_tag="PLG49_07245"
     CDS             9774..10496
                     /locus_tag="PLG49_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOQ17152.1"
                     /translation="MFCTKCGNPLEEGQKCACQQSHLKTAREIFKTEVKVFQEVNSTN
                     IKLFLLENLYTFFEFIKSPTIVIKRCAKKQDIADSIFFLSLQALLSGFFLILVTMKLD
                     DFASDLLGVFSFLWEAKIPYFTIFIKTVLYVFLLFILFNFVLYLFSNVLFKGEGNFKG
                     LVSTLGIIAIPKIITLWLSFILILISFKFVLCLNFMTYIFGMILNFVAVKEVFGFSED
                     KSAYALAFSYLLYFILIVVFIF"
     gene            complement(10559..11194)
                     /locus_tag="PLG49_07250"
     CDS             complement(10559..11194)
                     /locus_tag="PLG49_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOQ17153.1"
                     /translation="MTPRKEYYKQNAKKILTALAKRHMVGYYCETKEIALKKAFEIMQ
                     EGSTVAWGGSQTLKEIGILEELKKHNYTLLDRDSAKSPEELEKIYHDTFCADYYLMST
                     NAITLDGKLINMDGRGNRIAALIFGPKNIIVFAGMNKVVPNEEAGIERIKSLAAPMNA
                     IRLQTKTPCTATGHCQNCSSPSSICSNLLITRLSKTPNRIKIILIGEELGF"
     gene            11407..14067
                     /locus_tag="PLG49_07255"
     CDS             11407..14067
                     /locus_tag="PLG49_07255"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HOQ17154.1"
                     /translation="MKKFFNQSVETVLQELNSDMEKGLSKEEATKRLKEYGANQLKEK
                     EARGIFQMFLDQFKDFLVVILIVASIISILVGEVKDAAIILAIVILNAVLGVFQENRA
                     SNALKALKEMAAPLAKVLRDGNLEKIPSSDLVPGDIVLLDAGDYVPADLRLIETVNLK
                     IEEAALTGESVPVEKFADEMVEENAGIGDRVNFAYMSTVVTYGRGKGIVVATGMDTEI
                     GKVASMLEAVEEEQTPLQKKLNQMGKVLGSLCLAICAVIFLLGLLRGQELLEIFMTSV
                     SLAVAAIPEGLPTVVTVVLALGMQKMIKRNAIMKTLGAVETLGSTTVICSDKTGTLTQ
                     NKMTVVKAFDGEYLWDVTGRGYTTEGEIKNCDNPKVNTSPALELLLKAGVLCNDAFIK
                     KGEADIIGDPTEGALVVLGAKGGYLKDALLNEMPRIGEIPFDSDRKLMTTFHRQNDEM
                     IMFTKGAPDIVLNRCKYIYLNGEVVEITEEHKKMITEKNNEFAKEALRVLALSFKMPE
                     KINESINEEKDLIFLGLVGMIDPPRDEAKKAIAICKKAGIRVVMITGDHKTTGAAIGK
                     ALGIIEKDEEAMDGKEIDVLDDEALKEKVKYVNVFSRVSPEHKVRIVRAIRNNGEIAA
                     MTGDGVNDAPALKQADIGIAMGITGTDVAKEAADMILTDDNFASIVAAVEEGRTIFAN
                     IRKFVGFLLSCNIGELLLIFIAMLLGLPVPLLPIHLLLINLITDAFPAFALGVEPMEE
                     GIMDQPPRDPQEPIINKKMAIAVGIQSIFLASAALASFYFGFKYFTGENSLVLARTYC
                     FVTLVAGELLRAYSARSEKTTIFKMKLFENKYLNLSVLGSLALLFAIVYIPALQLVFN
                     TLPLSLNNLANALLFALIPVAGGEISKKFK"
     gene            14215..15078
                     /locus_tag="PLG49_07260"
     CDS             14215..15078
                     /locus_tag="PLG49_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ17155.1"
                     /translation="MLDIAIIGAGPAGLSAAINGVIRNKKVIVFGRDPKSSYLYKAEK
                     VDNYLGLPNISGPEMIHQFIKHAQDLGVEFHEGRVLEVFPMGDYYTLNAENEFFEART
                     VIIATGVPKAKYIPGEKELLGKGVSYCGTCDGPLYRGKTTIVIGEIEEAEEDVNFLRE
                     ICSKVYFLPTYKEVKYVPSDVEIIRGKPKEIFGDPFVSGIKIDDRELKVDGVFLIKET
                     IPVTQLIKGLEMDGKTIKVNRQQETNFPGVFAAGDCTGKPFQVTKAVGEGAVAALEAV
                     SYLHKLESKNK"
     gene            15306..>16132
                     /locus_tag="PLG49_07265"
     CDS             15306..>16132
                     /locus_tag="PLG49_07265"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOQ17156.1"
                     /translation="MKKKVHISQCAELDILFEDIVSQYGTLILPKGAVINEYIIQKLK
                     EFGIEYVYIFDRNREKIFPDYESFEERFDENIDKIKYLMNEIIRGKKLDYNELNRITD
                     DVIVAWDNPEYVMNYLKKIHDKDDYTYYHCINVSFYSALIASYLKLPKEEIKTVIQAA
                     LLHDLGKVKIPLELLNKKEKLTVEEFNEIKKHAVYGYEIVKNDDEILDAIKDVILMHH
                     EREDKSGYPLGLSGEELSIYSKIVSVADVYDAMTSNRPYRKALTPFDAFNEFVNISR"
BASE COUNT         5409 a         2326 c         3275 g         5122 t
ORIGIN      
        1 gctaattccg tttgtgaagc agcagaagaa ggagacgctg agattgctgt aatattaccg
       61 gcaaattcct gtatcacaga aatctttgtt tctggcagaa gtgctgatga ttttgattcc
      121 atacttataa cggttaacaa tggcacagaa aatattttct gtcaaagatt ttatgtagca
      181 gaagatgctg gagttgctgg agtaaacttt gaaacggctt taacacgacc gctttgtgta
      241 ggagcagggg gagcgaccgt aacggcaaca attgaaggcg gaactgctga tgaaggtttg
      301 ttaacggttg tatattgtga gagttgttgt tcagcagaag aataaaagaa taagaaatta
      361 agtggggaat catccccact taatttttta tagaagcgcc tgtaaaagtt acaggaccta
      421 taatattgat gttatcctca ttatcgaagg cttgcgcggt caaacagtaa gtgatttttc
      481 tacaaaatct tctgtttggt tgatccacat gggtaaaagt cgttgtcctg gatgttccat
      541 taccctgacc attgtcggcg gtagaaaaaa tcagttctcc tgtatctggc tgacctctcc
      601 agatggaaaa aactaaacgg gacggatcta aagaaaaaac ggaaagactg cctatcgtag
      661 cgcttaacca gacaaggggc gattctttag acacagggac tgtaatacag gagagggtga
      721 caggctcact ggcaggcggc agtatgacgg gtactctggg gtccgtacta ttggatgtgt
      781 tgggccttaa tatatcaaaa ataaactgaa tcagttttcc taaacggatt tttaagggat
      841 ttgccatttc agacctcctt tcttttttaa tttgaatcaa gaatttctgc agcagtaaag
      901 gtaatgggtc caatgatatc gatactttca atatcaacgg cttctacggt taaagtatag
      961 ctgacacagg aacaggtctt tttttgcgta tcgacatgat tgagattggt tgtacgaaat
     1021 gttggttcac ctgcaccccc ctgaccactg tcagttgttg tgtaaatcaa gtttcctgta
     1081 tcgggatttc ctcggtatat tctgaaaatc atagcggcag gtgggttgga ggcgtccgga
     1141 ccattgactc tgaaacctac agtagcactt aaccagatga aggatttttc tttttggacc
     1201 ggtaagcgaa tggttgataa gatgacttct cctgttgtat tgtccagagg aatgggtact
     1261 ctgggatccg tactgtcaga aatattggga tttagggtat cgtatgcaaa gttgctgagt
     1321 ctttcaatat taatttgaaa gcttgaagac atagataacc tcctttcatt tatgattcat
     1381 acttagatac tgacagcaat aaagctaatg ggcccattaa aggtcaattg agttgctaca
     1441 ttgctttcaa ccgtcaagta ataggtgatt ctggagcatt ttacattgat aagctgatcg
     1501 acagtggtaa aactactgtt acgaaaagtt ccgactgtta tactgccctg accattgtct
     1561 gctgtactaa agatttcaaa gtcattactt ggatggcctc tccaaattct gaatatagca
     1621 gcaggacctg ttaaatctga tcttgcacta aatcccaggc ttgcaaatag ccatacagcg
     1681 ttttttttct cagggatatc tatggtgatt tgggccagaa tcacaggggg atcagcagcg
     1741 gtgattacaa taggaactct ggagtctgtg ctatttgtta tgtttggatt taagacgtca
     1801 aaagaaaaat ccctgataat tccattcgtt atttgaaatg gctgtaaggt attcattaaa
     1861 aacctccttt ctttgacatt gttctaatat ataatatgaa gttgtcttac aaaatgttac
     1921 gaattatgtc acaagatgac gaaaaaataa tgaagcaaaa aaggtaaaaa aaaaagttag
     1981 gaaaaattcc taacttattt ttgaccaatt accgatatcg ctttaacaaa ggcccggttg
     2041 taatagggct gtgacaaatt atcaattttt acaccttttg aacttgtggc atggatgaat
     2101 cgattgttac caatatataa accaacgtga gatacttttc cgttgtttgt tccagaggtg
     2161 tcgaaaaaaa ctaaatcagc ggggctaagt tcttctcttt ttaccggaat tccgttcata
     2221 gcctgttctt tcgagcttcg ctttaaagag atatcaaatt ctttcatcac ggcctgggta
     2281 aatccagaac agtcaacacc tgtttctaaa gaagtaccgc catatttata aggtgttcca
     2341 ataaattttt tagcataatc aacaatttgc cttctgaata aatcttcact gctcactggg
     2401 gtaatctcag gtaaaagatg aagattaaat ccctcaacat attttgaaaa tatccaacct
     2461 gtttggtatt tgtaatgaat tctgtaccag tcctgatatc tgccggtaac taagataaca
     2521 tccaactgat tggctttagc taaaaccttt gaatcagtat ctggaaattg ccatatatta
     2581 atttcatttt cattgacgat agcaagtctt gaatctttat atctttccat agaccattca
     2641 gcttctttta tttcgccgta aatcaaggaa ttatttaaga taaaaatcat aaaaagggtt
     2701 attaaccggt taattttcat ttgatttaat ttctccatat tagcctccca gagattaatg
     2761 gattatttta gtattttttc ttagtatgat taattttttt caaaactatt ccatgttttc
     2821 aaaaagaaaa acaaaaataa ggattactat tgattatcag tcgataaaag tgctattata
     2881 acataagtac tgtgtataca acataaatcg ctataagaat tatatttttt ttgtagagaa
     2941 aattcttaga gagaaaaata tttttatttg cagaccagta atgaattttt gatggaggga
     3001 ttttatgaaa aaattgattt taggtaatga aggggtggca agaggtgctt atgaagcagg
     3061 tgtaagagta gcttcagctt atccgggaac gcccagtaca gaaataaccg aatttattgc
     3121 gaaatatgat gaaatttacg cagaatggtc tccaaatgaa aaagtagcta tggaagttgc
     3181 tattggtgct tctctggcag gagcaagggc catggtagct atgaaacatg tgggattaaa
     3241 cgtagcggct gatccgcttt ttacggtttc ctatacaggg gtaaatggag gcctggttat
     3301 tttagtggca gatgaccctg gtatgcacag ttctcaaaat gagcaagaca gccgttgtta
     3361 tgccagagct gcccatattc ccatgttaga acctgctgac agccaggaag caaaagattt
     3421 tgtaaaattg gcatttcatt tgagtgaaaa gtatgataca ccagtttttg tccgtatgac
     3481 aacgagggtt tcccatgccc aaagtattgt tgaatttgga gaaagagaag aaatcgcctt
     3541 aaagccttac gataaaaata ttcaaaaaaa tgttatgatg ccaggtatgg ccataaaaag
     3601 acatattgtc gttgaagaaa gaatgaaaca attagctgaa gatgccaatc aattagagat
     3661 caataaaata gaatggaacg ataaaaaaat tgggattatt accagtggca ttgcttatca
     3721 atatgttaaa gaagctattc caaacgcttc tgttttaaaa cttggaatgg tacatcctct
     3781 tccaaaaaaa ttaatagagg aatttgctaa tcaaatagaa actttatata tcgtagaaga
     3841 attggaacct gttatcgaag aacaggtaaa aagctatggt attgcggcca taggaaaaga
     3901 agtcttttca gtacagggag aattatcagt taaaaaaata ttatctgcta ttgctaaaac
     3961 agaaattcca tctaaaaaac cagaacagtt accaggtcgt ccaccggtat tatgtcctgg
     4021 ttgtcctcac aggggcattt attatgtgat gaaaaaatta aaattacatg ccacagggga
     4081 tattggctgt tatactttag gtgccatgga acctttatcc agtattgatt cctgtgtttg
     4141 tatgggtgca agtgttggta tggtgcacgg tatcgaaaaa gccagaggca gagaattttc
     4201 aaagaattgg gtaggcgtaa taggggattc tacttttatt cattcaggaa taacgggttt
     4261 aattgatatt gtatataata aaggcatgtc tacggtactt attttagata attcaacaac
     4321 aggtatgaca ggccaccagg atcaccctgc aacgggtaaa accattaaag gggaagcagc
     4381 accaattcta aatctggtgg aattagtaaa ggcaatagga attaaaaatg taagagtcgt
     4441 gaatcctttt gatttaaaag aagcagaagt tgtattaaaa gaggaaacca ataaagatga
     4501 gccttcagtg attattgcca gcagaccatg tgctttatta acaaaagaaa agaaagcagc
     4561 ttgcaaaatt acggatgcat gtgttatgtg tggcagttgt ctgcgattag gttgtcctgc
     4621 cattgaaaaa caagatggaa aagtagtcat tgacgatgca ctttgtaatg gatgtggatt
     4681 gtgtccaaaa gtttgtcggg taaatgctat tgtaaaggac ggtgaataag atggaaacaa
     4741 aaaatatact cattgtcggt gtcggaggac agggaacctt attgaccagc agaattattg
     4801 gggatgtagc tgtaagaaaa ggttatgatg ttaaaatgtc tgaagtgcat ggtatggctc
     4861 aaagaggggg tagtgtggtt acccacgtaa gatatggcaa gaaggtttat tctcccttag
     4921 tggaagaagg aacttgcgat attcttttgg cctttgaaaa attagaagcc ttacgttgga
     4981 ttcatttttt aaaagaagat ggaattatta tcgtcaataa tcaggaaatt aatcctatgc
     5041 cggttattac gggagcagcc cagtatccca cggatattat tcagagaatt cagagggctt
     5101 gtaagcaggc gtatatatta gatgcactta gtattgctaa agcattagga aatattaagg
     5161 tcgttaatac cgttttatta ggggtattgg caaaacattt aggtcttgaa aaaagtgatt
     5221 gggaagaagc tatcgagcgg actgttcctc caaaaacagt tgaattaaat aaaaaggcct
     5281 ttttgacagg ttatgaatca gaaataaaat aagggtgttt atcaccctta ttttttaata
     5341 tactgtgatg gttactttaa cggtatcttt ttctgtaaat cctttaataa agggtttgtt
     5401 ctgaggccca aataccctgc tgtcttcatc atcgtaaatg atggtttgga caaaggcatc
     5461 ctgagcaaca ccggcataat caattttata tttatcgcct ttttttgcac ctggaataga
     5521 aatgaggaat tcgaagggga tccaactgtt ggaatgttgg aatttttatc tgctaaatgg
     5581 tgtaattttt cttttttatg aggggggagg tgttcatctt tatgatgtaa tggtgggtgt
     5641 tttttgatgt gtggagctgg tggagggatg ctttttacaa caattttgtt atgtctggta
     5701 atgggctgat ggatggggtt gtatattttt ttagtaaaaa aataagtata gagtataagt
     5761 aaaaaaatat aaaaaaagca aggcagaaat agctttgtaa gaaaatcaaa aagggtaaaa
     5821 tccgtatata gtgttaatat tacagctggt gtatcagccc agggtaatgc ggcacctcct
     5881 atattagacg cgataatagc agataccaaa acaggaagga tggatgtttt aaaatgtatt
     5941 gctagttcga tgcagatgaa agcaaataat aaaatgacag ttaagttatt cataaacagg
     6001 gataaaaaat agatagtaaa cattaaaata gaaagagcaa gaaccttttt accccgggaa
     6061 gtttcaatca gccacttttt tatataatca aagacgccca ggttttcaaa agtttttgta
     6121 aaaagggata gtacaaaaat aaggataatt atttccacag ggaattcttt caggatactg
     6181 tctgcattaa aactgccgct tacagaaccg taaattaaga tagcagaaag gcctaaaaca
     6241 gcgataggaa gtcggtattt tgtaataaaa atcatcatgc aataagtgat tagaacagcg
     6301 atgataatga tagacaaatg ctcaccccct tgtcagtctt gatgacagta atttgttgaa
     6361 atatttttta tactacaata tatgagctta gacaagggga tgtgacgaat cattgcctat
     6421 tttgtctttt tgtgtgataa ggtgtcaaag gaagaaataa aaatgatggc aatggcaata
     6481 gctccggcga tttcaaaagt ttgtgcccca taaaatgcag cttctttaaa attttgattg
     6541 agtaccgcat acatggtttt atacattcct gcacctggaa caagggggat aatacctggt
     6601 attaaaaaaa tagtgatagg catttttctt aacttggcca gtattcttga tataatacta
     6661 atactgagag cactgataaa attagaaaaa ataacagagt cagtccactt aaaaataaaa
     6721 agatatatta accagcctaa agaaccaacc aaaccgcaat agcccagttc ttttttagaa
     6781 acattaaaaa ggattgcaaa gaaaaaagtt gcaaaaaaag cactgataat ttgtaaaatc
     6841 atgtgaacct ccatttattt ataagcttta agattaaaaa ggaagccata ctttaagggc
     6901 aatagcaacg cctgtggcaa tggatatggc cacgaaaaag gcttctacac ctctggatat
     6961 tcctgataat aaattacctt caatggtatc tcgtatggca tttgttatgg caacgccagg
     7021 caccataggc ataatagagc caatgatgat gttgtcaaag tttgtagaag gaatgatgga
     7081 agttaataaa agggctaaaa agccaactaa aaatccgcca agcatatccg ctagaaaacg
     7141 ggatagttgc aaatttataa ataatcgttg tataattcct aaaataattc ctatacttaa
     7201 tgatatcaga aaatctttat aatttccccc aaagacgatt gtaaaaaatg cagaagtcaa
     7261 gcctgtcaca ctaataagaa ttaagggttg gtagggagaa attttgtcaa ttttttttag
     7321 ttcttctatg gcgtcttgca aagagatttt tccaagaaca tactttctgg acaaatcatt
     7381 gaccagggca actttatcca gccggatggt cctgctgtta attcttttta gtttggtagt
     7441 catttcgatg tttttgtcat cgatggttgc aataattcct gtaggagtaa caaaaacttc
     7501 tatactttca aagtcataaa ctgaaagaat ccgcatgatt gtatcttcaa cccggtaagt
     7561 ttctgctccg cttctaagca taatttctcc agcttgcatt gcaaaattta ataataattt
     7621 gtggtccata agcaaactcc ttttctgata tagaaaaagt acctgtaaag gtacttcata
     7681 ggtttgttaa tattatgaga tcaaatatat aaattagttt acgttattta tttggtaaaa
     7741 gaagaaggtt ttctttacag aatgataaga aattctttat aaatatatta attcatctgc
     7801 agctatattg caaggttaaa gtatatttta aaatttagga ctattaaaaa gcatttaatt
     7861 ttttttctta ttgtgttata attttcatgt gaaattggag tgcaaagcac tttcaaatat
     7921 catacttagg aggtaatatt tactatgtac tcgtttgaag aaatacaaaa agtcgactta
     7981 gaagtagcag aggctataga aaaagagatt aacaggcaaa ataataatat tgagcttatt
     8041 gcctctgaaa attttgtatc aaaagctgtc atggcagcta tgggctctcc attgaccaat
     8101 aaatatgctg agggatatcc tggcaaaaga tattatggtg gatgcgaatt tgttgatgtt
     8161 gttgaaaatt tagccattga aagagcaaaa aaactttttg gtgccgagca tgttaatatt
     8221 caggcccatt caggggcaca agcaaacatg gctgtctttt ttgccgtatt aaatcctggg
     8281 gatacagtca tgggaatgaa tctttcccat ggaggacatt taacccatgg cagtccggta
     8341 aacatgtcag gaaaacaata taatatcatt ccttatggtg tagatgaaaa gacaggctat
     8401 attgattatg atgctttgat ggaattggct aaagaacata aacccaaatt aattattgca
     8461 ggtgccagtg cctatccaag aattatagat tttgaaaagt tcagagctat tgctgatgca
     8521 gtgggtgctt atttaatggt agacatggcc catattgcag gtttggttgc agcaggtctt
     8581 catcctaatc cagttcctta cgcggatttt gtgacgacca cgacgcataa gactttaaga
     8641 ggtccaagag gtggaatgat tctttgcaaa gaagaatttg ctaaaataat tgataaagca
     8701 attttccctg gtactcaagg cggacctttg atgcatgtta ttgcagcaaa ggcagttagt
     8761 ttaaaagaag cagcaagcga tgaatttaag gcttatcaaa aacaaatcat aaaaaatgct
     8821 aaagccttgg cagaggcctt aatggctaag ggatttaatt tagtttcagg tggaacagac
     8881 aatcatctga tgatgataga tttaagaaat cttaacataa caggaaaaga agctgaaacc
     8941 cgattagatg aaattggtgt aacttgcaat aaaaatacaa ttccttatga tcctcaaagt
     9001 ccttttgtga ccagtggaat acgccttgga acaccagcag tgacaacaag aggtatgaag
     9061 gaagaagata tgcaggttat tgcagatatt attcacatga ccttaaccga ttttgaaagc
     9121 aacaaggaaa aagcaagaga acttgtagca gctttagttt ctaaatatcc tttgtactaa
     9181 agtagggaac attgtttcct actttttctt atctgttatt ctattaaaaa atggaggtca
     9241 atatgtatat tcatgaaaca aaaataaaag ttcgttatgc tgaaacagat caaatgggga
     9301 ttgttcatca ttccaattat tatatttggt ttgaattggc caggacagaa ttttttgaag
     9361 caattgcttt gccttatgac ctgatagaaa gagacgggat tatgtttcct ttggtagaaa
     9421 gcagctgtat ttataaagaa tctgcaagat atgctgaaaa tcttattatt aaggcatgga
     9481 tagaagaaat aaaaggaatg aagtttactt ttttatatca agtgattagg gaagaagaca
     9541 aaagagttct cgctatagga aaaaccattc atgttcctgt agataaaaat atgaagctga
     9601 taaatttaaa aaagaaaaat cccagactgt tagaaaccat aaaagccgtt atttcagcaa
     9661 aataattaaa aatatagaaa agtcttatat ttgcttgaaa aaaatgtaaa aataatgtag
     9721 tattatgata tggatggaaa acaattttat tattttaggg ggcatgtatt atcatgtttt
     9781 gtacaaaatg tgggaatcct ttggaagaag ggcagaaatg cgcatgtcag caaagtcatt
     9841 taaaaacggc cagggaaatt ttcaaaactg aagtgaaggt ttttcaagaa gttaattcta
     9901 caaatattaa attgttttta cttgaaaatc tttatacatt ttttgaattt attaaatcac
     9961 caacgattgt tataaagcgc tgtgcaaaaa agcaagatat tgcagatagt attttctttt
    10021 tatctttaca ggcactttta tcgggttttt ttcttatttt agtaacaatg aaattagacg
    10081 attttgccag tgatttattg ggcgttttta gttttttgtg ggaggcgaaa attccatact
    10141 ttacaatatt tattaagacg gttttatatg tttttctgtt atttatttta tttaattttg
    10201 ttctttatct tttttcgaat gttcttttta aaggagaagg aaattttaaa ggattagtaa
    10261 gtactttagg aattattgcc atacctaaaa taataacgct ctggttatcc tttattttga
    10321 tacttatttc ttttaagttt gtattgtgct taaactttat gacctacatt tttggaatga
    10381 tattaaattt tgttgcggtg aaagaagtct ttggatttag tgaagacaaa agcgcctatg
    10441 ctttagcttt ttcctacttg ctatatttta tattaattgt ggtatttatt ttttaatttt
    10501 tgttaaagta aggtaagaaa aaaacccgtt aatacgggtt ttaattttca ttatgatttt
    10561 agaatcctaa ttcttctcca attaagatga ttttaatacg gttaggggtt tttgacaggc
    10621 gggtaattaa aagattggaa cagatagagc ttggggaact acaattttga caatgaccag
    10681 tggccgtaca aggtgttttt gtttgaagcc ggattgcatt cattggggca gcaaggcttt
    10741 taattctttc aatgccagct tcttcattgg gaacaacttt attcatacca gcaaatacga
    10801 ttatattttt tggaccaaat attaaagcag caattctatt tcccctgccg tccatattaa
    10861 ttaattttcc atctaaggta atggcatttg tactcattaa ataataatct gcgcaaaaag
    10921 tatcgtgata gattttttct aattcttcag gagattttgc actatctcta tctaaaaggg
    10981 tataattgtg cttttttaat tcttctaata taccgatttc ttttaaggtt tgagaccctc
    11041 cccaggcaac agtagaacct tcctgcatga tttcaaaagc tttttttaaa gcaatttctt
    11101 tggtttcaca gtaatatcct accatatgcc ttttggcaag agcagtaagt atttttttgg
    11161 cattttgctt gtaatattct tttcttggtg tcatttcgat tcccctctcc ttttttctac
    11221 attattatta taacgtatta ttgctatggt tttcaaatga ctttgtattt ttgaaaaaaa
    11281 tcattaaata ataaaagggg atttataaat tttcaaaaat ctagtataat cttatagtgt
    11341 aaaattttat ttattttata attaaaatag gattgtataa attttatagg aggcaagaga
    11401 taaataatga aaaaattctt taaccaaagt gtagaaaccg ttcttcaaga attaaatagt
    11461 gatatggaaa aaggactgag taaagaagaa gctactaaac gtttaaaaga gtatggagcg
    11521 aatcagctca aggagaaaga ggccagagga atttttcaaa tgtttctgga tcagtttaaa
    11581 gattttttag tagttatttt aattgttgcc agtattattt caattttagt aggtgaagta
    11641 aaggatgcag ccattatact ggctattgtt attcttaatg cagttttagg tgtttttcag
    11701 gaaaaccgcg caagtaatgc tttaaaagca ttaaaagaaa tggccgctcc tttggctaaa
    11761 gttttaagag atggtaatct tgaaaaaata ccatcttcgg atttagtacc tggagatatt
    11821 gttttattag atgccggtga ttatgttcct gcagatttaa gattgattga aacggttaat
    11881 ttaaaaatag aagaagcggc tttaacagga gaatccgttc ctgttgaaaa gtttgctgat
    11941 gaaatggttg aagagaatgc agggatagga gacagagtaa attttgccta tatgagcaca
    12001 gtggttacct atggaagagg aaaaggtatt gtcgtggcaa caggtatgga tacagaaatt
    12061 ggtaaagttg cttcaatgct ggaagctgta gaagaagagc aaacacctct tcaaaagaaa
    12121 ttaaatcaaa tgggcaaagt ccttggaagc ttatgtttag ccatttgtgc ggttatattt
    12181 cttttaggct tattaagagg ccaggagctt ttagaaatat ttatgacttc ggttagtttg
    12241 gcggtagcag ctattcctga aggccttcct actgtcgtta cagttgtttt ggctttagga
    12301 atgcaaaaaa tgattaaaag aaatgcaatt atgaagactt taggtgctgt tgaaacttta
    12361 ggaagtacta cagtgatttg cagtgataaa acaggaactc ttactcaaaa taagatgaca
    12421 gtggttaaag cctttgatgg ggaatacctt tgggatgtaa caggaagagg ttatacaaca
    12481 gaaggtgaaa tcaaaaattg cgacaatcct aaagtgaata cttctcctgc ccttgaattg
    12541 ctgcttaaag caggggtact ttgtaatgat gctttcatta agaagggtga ggccgatatc
    12601 attggagacc cgacagaagg ggctttggtg gtattagggg caaagggagg ttatttaaag
    12661 gacgccttat tgaatgaaat gccccgtata ggagaaatcc cttttgattc tgacaggaaa
    12721 ctcatgacga cttttcatcg acaaaatgat gagatgatta tgtttactaa aggggctccg
    12781 gatattgttt taaatcgctg taaatacatt tatttaaatg gtgaagttgt agaaattact
    12841 gaagaacata aaaagatgat tacagaaaaa aataatgaat ttgcaaagga agctctaaga
    12901 gttttggctc tatcctttaa aatgccggaa aaaattaatg aaagtataaa tgaagaaaag
    12961 gacttaattt tcttaggtct tgttggtatg atagatccac caagagatga agccaaaaaa
    13021 gcgatagcaa tttgtaaaaa agcaggtatc cgggttgtaa tgattaccgg agaccataaa
    13081 acgactggtg ctgcaattgg taaagctctg ggtattattg aaaaagatga agaagcaatg
    13141 gatggaaaag aaatagatgt tctcgatgac gaagctttaa aagaaaaagt taaatatgtc
    13201 aatgtgtttt ccagggtttc tccagaacat aaggttagaa tcgtaagggc cataagaaat
    13261 aatggagaaa ttgcagcgat gacaggagat ggagttaatg acgcacctgc cttaaagcaa
    13321 gcagatatag gtattgcaat gggtattaca ggtacagacg tagcaaaaga agcagcagac
    13381 atgattctta ctgacgacaa ttttgccagc attgttgctg ccgttgaaga aggaagaact
    13441 atttttgcta atataagaaa gtttgtagga ttcttattat cctgtaatat aggagagtta
    13501 ctgcttattt ttatagcgat gttactgggg cttccagtac cccttcttcc aattcatttg
    13561 ctgttgatta atctgattac agatgccttt cctgcttttg cattaggtgt agaacctatg
    13621 gaagaaggta tcatggatca gcctccaaga gatcctcagg aacctattat taataaaaag
    13681 atggctattg ctgttggtat tcaaagtatt tttttagctt cggctgcatt ggcatctttc
    13741 tattttggat ttaagtattt tactggtgaa aatagccttg ttcttgcaag aacttattgt
    13801 tttgttactt tggttgcagg agaattattg agagcatatt cagcccgttc tgaaaaaact
    13861 actattttta agatgaaatt atttgagaat aaatatttaa acttaagcgt gttaggctct
    13921 ttggcattac tttttgccat tgtctatatt ccggcacttc agcttgtatt taacactttg
    13981 cctttatccc tgaataactt ggcaaatgcc ctgttatttg ccctaatccc tgtagcagga
    14041 ggagaaattt ctaaaaaatt caaataagct gcacatggga caaagcagaa aaatacaata
    14101 taaataggat gtaatattgc aatattatga tatatatgcc ataatattag gtacaacaaa
    14161 attatttgtt ttagagtaaa ggattatgcc tacagggcag aacaggaggg taatatgctg
    14221 gatattgcga ttattggtgc agggcctgct ggtctttcag cagccattaa tggggtcatt
    14281 agaaataaaa aggtgattgt ttttggaagg gatcctaaaa gcagttattt atataaggct
    14341 gaaaaagtgg ataattattt aggtttgccc aatataagcg gtccggaaat gatacatcag
    14401 tttataaaac atgcccagga tttaggggta gaatttcatg aaggcagagt attagaagtt
    14461 ttccctatgg gagattatta taccttaaat gcagaaaatg aattttttga agccagaaca
    14521 gtaattattg caacgggagt tcctaaggct aaatatatac ctggagaaaa agaactttta
    14581 ggtaaaggcg ttagttattg cggtacttgc gatggtcctt tgtatagagg caagactact
    14641 attgttattg gagaaataga agaagcggaa gaagatgtta attttctcag ggagatctgt
    14701 agtaaagtct atttcttacc gacttataag gaagtaaaat acgttccttc tgatgttgaa
    14761 atcataagag gcaagccaaa agaaattttt ggagatccat ttgtttcagg gattaaaatt
    14821 gatgataggg aattaaaagt ggacggggtt ttcttaatta aagaaaccat accagtgact
    14881 caactgatta agggtttgga aatggatgga aaaacaataa aggtcaatcg tcagcaagaa
    14941 actaattttc ctggggtttt tgcagcagga gattgtacag gaaagccctt tcaagttaca
    15001 aaggcagtag gagaaggtgc agtagctgcc ttagaagccg taagctatct tcataaatta
    15061 gaaagtaaaa ataaatagat ttatcttttg ttgaatattt ttttaaaaat aaaaaagcta
    15121 ctagtccatc aagtagcttt tttattttgg caatacgaca taattctgct aagattgaca
    15181 caatactaaa cctcatgaca aatattgaca aacagaatta ctattgttat aataatttta
    15241 actatagtaa ataattaaag attcatttaa ttatttaaaa tgtaaaatat ttggagttga
    15301 aaggaatgaa aaaaaaggta cacatatctc aatgtgcgga gttagatata ctttttgaag
    15361 atatagtgag ccaatacggg acattaattt tgccaaaagg tgcagtaatt aatgagtata
    15421 tcattcaaaa gttaaaagaa tttggtattg aatatgtcta catttttgat cggaacaggg
    15481 agaaaatatt tccagattat gaatcttttg aagagcgttt tgatgaaaat attgataaaa
    15541 ttaaatattt aatgaatgag attataagag gtaaaaagct ggactataat gaattaaata
    15601 gaattacaga tgatgtgatt gttgcatggg ataatcctga atatgtaatg aactatctta
    15661 aaaaaatcca cgataaggat gattatactt attatcactg tatcaatgtt tcattttatt
    15721 cagctttgat agcaagttat ctgaagcttc ccaaagagga aataaagacg gttattcagg
    15781 cagctttatt gcacgatttg ggtaaagtaa agattccact tgaactttta aataagaaag
    15841 agaaattaac cgttgaagaa tttaatgaaa ttaaaaaaca tgctgtttat ggttatgaaa
    15901 tcgtaaaaaa tgatgatgaa atccttgatg cgattaaaga tgttatttta atgcatcacg
    15961 aaagggaaga caaatcaggt tatcctctgg gtttaagtgg agaggaattg agtatttatt
    16021 cgaaaattgt ttctgttgcg gatgtatatg atgctatgac ttccaacaga ccatacagaa
    16081 aagccctgac accttttgac gcttttaatg agtttgtgaa tatttccagg aa
//
LOCUS       DAOSUD010000049        15402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2346_length_15402_cov_22.200821, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000049 DAOSUD010000000
VERSION     DAOSUD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_15402_cov_22.200821"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1021)
                     /locus_tag="PLG49_07270"
     CDS             complement(119..1021)
                     /locus_tag="PLG49_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ17157.1"
                     /translation="MVSINCLDIISLLSLEGIGRKGVGLILKQYRNISLSIKDIYEIL
                     KDKNYYFTLKELYQVYEKSKNIIEELNQSKIHMIDCLSTAYPDSLRKIEDPPLLLFIK
                     GYYDFIYNEKNCLAVIGSREPTKKGKKTAYNIARYLARKKFSIISGLALGCDTQAHLA
                     CLKEKGRTAAILAHGLNMIYPKENEKLAQDIIDKGGCLISEYLPYEKAKNYTFIERDR
                     LQSALSQAVIVIETEETGGTMHTVRFAKKQNKLLACVQYTPEQMQDYKLSGNKIILNE
                     LNAIPVRNYEDLDDLITYLRENKS"
     gene            complement(1106..2752)
                     /locus_tag="PLG49_07275"
     CDS             complement(1106..2752)
                     /locus_tag="PLG49_07275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOQ17158.1"
                     /translation="MRRFEFSEKKYTNINIYLFLLIIIYLEINKISGVLTDLGDALIF
                     LLAGILIMILKTYFIEKGYLEKKYLYLAFKIAELLVISYLIYYHEIISLIASVIYLLI
                     VAEMILQDFRHKSVFISSFFPIIMVGLLKLKTEFFNYEILINVSCIILISIIFIYVID
                     STLTNFKEDAEKDKQLLNELQQKNKELYKKNNEVELLNKELQEQKEEMKKTVENFRNS
                     VAELFILREISSYIGSILDIEQLLEMVCDMIMGIMGVDTCSIIVYNEKDGSLDFHIKS
                     IYSQDVIEKFKEQVWSSFIHSKIKNKEIIMDNNSSEEKYEFLKGRKVGSLVAVPLYKA
                     NKTYGLILAEHSLENYFSVSSTDLFKAVSMQVTMAIENAKLYEQMEDIAIKDGLTKIY
                     NRLYLQNILPQLVGEAIANKKPIAVSIFDIDHFKQFNDTYGHLFGDEVLKTVARFGQE
                     MVDQYDGIIARYGGEEFFIILPDMPLRKAAKVIEEFRKKIENYELRYNGQNAKITASF
                     GVSGYPEVADNVEELIKTADDAMYLSKRQGRNLVSIAPQI"
     gene            complement(2749..4977)
                     /locus_tag="PLG49_07280"
     CDS             complement(2749..4977)
                     /locus_tag="PLG49_07280"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOQ17159.1"
                     /translation="MILNYNIKEEDLVFGLDIGTRKVMGVIGYQEDDNFVVVDSAVVA
                     HESRAMIDGQIHDISKVANAVIKVKNILEERTGIELTDVSIAAAGRLLKTYLVKAEIH
                     FEEEKEIQRDLIRELELKGISEAQQKIKEDYKDDEIEYYSVGYSVVNYYLDDYIISNL
                     EGHIAKSIGADVLATFLPKAVVDSLYSVMDRVGLVVSNLTLEPIAAINVAIPEKLRLL
                     NLALVDIGAGTSDIAITKDGTVIGYGMIPIAGDEITEQIVHKYLVDFNTAEEIKYKIG
                     KESEITFTDIMGVSHTVSSEELYEVVKGPVSRLAEEISAKVCEVNANKKPNAVFCVGG
                     GSQVVGLTKKISEILNIPNERVAVRGGDSLINVKYLSETLNTPDVITPIGICVTALKQ
                     RGFDFIKVRFNKEEVKLLNAKRLTVLDVCMQKGLDHTNLLGRKGASLFFKLNGERRRI
                     KGGPAEPAELFVNGKEASLDTIIHDGDEILVQPAVNGEDASAFIRDFIDPKNLKRVLI
                     NGAYMELTSICYINGKLVDKNHEIFNNDDVKILSINTIADLADYLDVPLYDKEVYVNK
                     VKVDSSYIIRDGDSIEFKIIENKSFSQDVVDNNEIKISAEENKNTAGISYNMRSSHLT
                     ASNEEIEQKAVNPTTVTRDHTSEGLVFEDKMTQADELENKKTDIPVIVTVNGEKVIMK
                     SEKADYIFVDVFNFIDFDLSHPQGNIILKLNGNRAAFTDSIKNGDVIEIYWEKTNKVD
                     IL"
     gene            complement(5156..6808)
                     /locus_tag="PLG49_07285"
     CDS             complement(5156..6808)
                     /locus_tag="PLG49_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ17160.1"
                     /translation="MSRKLKSLMLAFFLILNMTSCTYNEDNNSIDPQETEEEQTVELP
                     EPTKGGEMIISFRMPKTLNPLLNEDYTVDQVLKLVFDTLIDFDESQKPIANLATDWTF
                     SNDGTTITINLRQGVQWHDGDPFTARDVIFSLDVIKNAPSTSPYKKCIENIVSYKAEG
                     DYTVKIFYNQPFSGAVYALYFPVIPAHIYETSSGQALLEKVPVGTGAYKLGEYKVAKE
                     LQLEVNPAWFKGEPYIERIKVLVIPDEETELYSFEQGQIDLIGTNAVDWGKYAQSGES
                     RVKEYVTSYYDFIGFNFNKVLFQDKKARKAVAYAIDKQNILENQYLNHGVLTDVPISP
                     TSWLYCDEIEKYNYDIEKAKELLAESGWSDSDNDGVLDKTMNGSKIDFIFNLLVESED
                     ETRKEAAREIIKMLSELGIKVNLEEVSKEEFLIRLQEKNFDAFIGGWKLSPIPDFTFA
                     FHSSQIENGSNFISFRSEQMDSLLEKAFTAVGEENMKMAYDELQKYIADELPYISLYF
                     RNAALITHDRIQGELKPQRDFYIGNIKDWFIYEPEKNEQSGQ"
     gene            complement(6833..7426)
                     /locus_tag="PLG49_07290"
     CDS             complement(6833..7426)
                     /locus_tag="PLG49_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOQ17161.1"
                     /translation="MFYIIDLRKKKFRFLFFLMLFIFFFYAGLELIKIIFYPLKYDHL
                     VLKYSELYDLDPYLVFSIIKVESKFDPNAQSHKNAKGLMQITEKTGEWAAAEIGIEEY
                     SSEKLFDPEINIHIGCWYIKKLLDQFEDIETSLAAYNAGSGNVSKWLQDKNYSKDGKK
                     VHNIPFLETKAYVKKVLDSRRIYKKLYKSIYNNQKLQ"
     gene            complement(7449..8045)
                     /gene="coaE"
                     /locus_tag="PLG49_07295"
     CDS             complement(7449..8045)
                     /gene="coaE"
                     /locus_tag="PLG49_07295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907570.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOQ17162.1"
                     /translation="MKVIGLTGGSGSGKTTVAVLLSQLTSVCIIDADKIGHEILKKGK
                     PAYDAVLERFGEAILKADGEIDRKKLGQIVFANQSLLEILNKITHPLIIQEIIERIHQ
                     IKSSDKACKYIIVDAALLIESGLHKAVDEIWLVYAPEEIRLKRIAQRDGMTTEEAKRR
                     IASQMPWEEMKKYGDVIIDNGHNEAYTMKQLNHIISSG"
     gene            complement(8057..10720)
                     /gene="polA"
                     /locus_tag="PLG49_07300"
     CDS             complement(8057..10720)
                     /gene="polA"
                     /locus_tag="PLG49_07300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ17163.1"
                     /translation="MNKIMLFDGNSIVNRAFYGVPLLTNSQGTYTNGIYGFLNILFKL
                     IEEDQPDYIAVAFDLKAPTFRHKVFKEYKGNRKGMPDELVVQIPILKQLLEAMGITIL
                     EMEGYEADDILGTLAKAAEKNGMSPIVVSGDRDLLQLASDITKIRIPKTRGSKTEIED
                     YYEKDLIDKFGVTAQEYIEVKALMGDSSDNIPGVPGIGEKTAYKIIHEYHNIENAIAN
                     SEKIKPARVSENLRVYEEQARQSKYLATICTDVPIEINWESLKMHELMNPKVYEWFKQ
                     LEFKSFFDRFKTTVARAANPSKNRNHKKVDGINELTEVIKDILLKGEFAFLIFKENHK
                     ILGISCCYDGSDGIWIEASPTLPIEEIMNKTKELFESRKIKKITHDAKSTMHILSRFG
                     ISLNNLSFDTMLGAYILNPIKDSYGFDDLAHDYLGEIFPSEEELLGKGKNKKLLSDLE
                     ENKITDITSEQACIVFHASKIMEEKIKENNQEFLYYEIELPLIEVLFSMEKHGFKIDI
                     NRLKEYIEELTEKIDILTNNIYNLAGEKFNINSPKQLGSILFEKLMLPVEKKTKTGYS
                     TAAEVLEKLKNKHPIINQILEYRQLVKLKTTYGDGLYAAVNKETGKLHSTFHQTITAT
                     GRISSTEPNLQNIPIKLEMGRKIRKVFIPQSKEYLLLDGDYSQIELRVLAHVAQDETL
                     IDAFRSGADIHKITASKVFKVPVEEVTSLQRSNAKAVNFGIIYGIGAFSLSQDLNISR
                     KEAEEYIESYFEKYPKVKEYMNRTVENAKESGYVTTLFGRRRPIPEISSKNFNLRSFG
                     ERVAMNTPIQGTAADIIKIAMVRVYKKLKERNLRSRLILQVHDELLVEVHKDELEEVK
                     EILKNEMENAVKLDVPLEVDIHFGDTWFEAK"
     gene            complement(10740..11600)
                     /locus_tag="PLG49_07305"
     CDS             complement(10740..11600)
                     /locus_tag="PLG49_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17164.1"
                     /translation="MKKWNILYILSAIIIIILIFLIFWNHSSKKSYETEIHFMEIPIG
                     HCTLIKIKERNILIGEVSEKDTKHIKEYLNSQKVNTISELIIASPKKDKIQGFESLLD
                     DYTIEKIYLPHISDLDKETEQFLYKAKEQGISIYQINSGEEWSVGEAKLLFLRPSSDT
                     TIPVSERKIIVKLILPKKSILLLPDMEKMDMSDLMLSSSKISSDIITINGNKRIEVLE
                     EDFLNAVKPDLIVFTNNARVKEKELQKVYNWNSSAKVISIYEEGYVIFYGGKGKYSLT
                     SRRTLNIKVQ"
     gene            complement(11711..12427)
                     /locus_tag="PLG49_07310"
     CDS             complement(11711..12427)
                     /locus_tag="PLG49_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOQ17165.1"
                     /translation="MKKTIKLLSVLLLAVFFTTASLSSPAVVKAETGINKITVRGEGV
                     IKVKPDMAYITLGVRTENKDAKQAQKENANKMNQVIAAVKNMGIDEKDIQTSGYSVYP
                     QYDYESKTGERIVGYTVTNTISVTVRDISKVGELLDLAVKEGANLSGGIQFSVSDTEK
                     YYNEALKQAIKNAQGKAAAIGEALGVVIKAPVSVVETSYGGRSVVYADEESFKTNAVS
                     TPVEIGEMDINAIVEVIYQY"
     gene            complement(12427..13719)
                     /locus_tag="PLG49_07315"
     CDS             complement(12427..13719)
                     /locus_tag="PLG49_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOQ17166.1"
                     /translation="MICEQYREWISFYIDGVLENEEKIAFENHLKTCKECRQELEEIQ
                     FLIEEIQKIPEKTLPEDYHKDLMLKLYKERDEKKSEKKKLDFNLKKLTAVAASFLLLF
                     FIMTSIGNMGMKSEQNGVAGEFEAAKQSYSKNEIASSDSFKSSAELAKTDMASDYGGM
                     AADYSMESLEEESINQAGNTFNAVSVDAGERKIIYNAYLSLEVELFDDTVKAIRELTQ
                     KSGGYVENFSSYIYYSEPERDIYLKEGTIVIRIPVEKYGDVSNELVKFGHLISQNEMT
                     ENITEQYIETESRIRMLELEQERLLEIMEKAEEVEDLIKLEERLNQVRTNLEIYKSMI
                     KNWDKLVEFSTFTITIKEVKTIEPIKMADPNLMARVKSNFNRSINDVRQTFEDFVVSL
                     SYALIPLLLLIVILGITFMIARPIFKKIIKMIHKRRDE"
     gene            complement(13719..14336)
                     /locus_tag="PLG49_07320"
     CDS             complement(13719..14336)
                     /locus_tag="PLG49_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ17167.1"
                     /translation="MEGKEEKLLVKRAKKGNISAFEELIIEHESKVYNIAYRILNNEE
                     DAKDLSQEVFIKAFNSLHKFREDSSFSTWLYRIAMNTCIDELRKRKGRESYSIDRDIQ
                     TDEGTIPREFQDKQLNPEEELMNKELASQIQWALNHLSETYKEVIILRDFQGFDYKQI
                     SQILGCSLGTVKSRISRARTELKNVLIKEKHFDFKNRLKTGKEGN"
     gene            14492..14575
                     /locus_tag="PLG49_07325"
     tRNA            14492..14575
                     /locus_tag="PLG49_07325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14526..14528,aa:Leu,seq:caa)
     gene            complement(14620..15090)
                     /locus_tag="PLG49_07330"
     CDS             complement(14620..15090)
                     /locus_tag="PLG49_07330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ17168.1"
                     /translation="MIEAVSCGGVVIHRGKILLLYKNYKHKYVGWVLPKGTVEPGEMY
                     RQTALREVYEETSVAAKVIRYIGHSQYTFQGKDDIVNKTVHWYLMRANSFYCKPQKEE
                     YFVDAGFYKFHEAYHLLKFNDERQIMKKAYYAYMELEKKKQWIRPKSYRKNVYK"
BASE COUNT         4660 a         3016 c         1979 g         5747 t
ORIGIN      
        1 gtttttacaa ttttagcaca ttgtttgttc tttatcaatg tttaattgtc cattactgat
       61 gatgttttaa aaaaacctta aatatttttc tggtgaattt ttactcttaa cactggtctt
      121 aggatttatt ttcccttaaa taagttatta aatcatctaa atcctcgtaa tttctgacag
      181 gtatcgcatt aagttcattt aaaatgattt tgttcccgct taatttataa tcttgcattt
      241 gctctggggt atattgaaca cacgctaaaa gcttgttttg cttttttgca aaccttactg
      301 tatgcatggt tccaccagtt tcttcagttt caataacaat cacagcctga cttaaagcac
      361 tttgcagcct gtccctttct ataaaagtat aattttttgc tttttcataa ggcagatatt
      421 cgctaatcaa acaacctccc ttatcaataa tgtcctgagc caatttttca ttttctttag
      481 gatatatcat attaagacca tgagcaagaa tggccgctgt ccgtcctttt tcttttaaac
      541 aggctaaatg tgcttgtgta tcacaaccca aagctaaacc actaataata gaaaatttct
      601 ttctggctaa ataccgggct atattataag ctgtcttctt ccccttctta gttggttccc
      661 tactaccaat gacagccagg caattttttt cattgtaaat aaaatcataa tatcccttaa
      721 taaacaacaa aagaggcgga tcttctatct ttctcaaaga atccggatat gctgtactta
      781 aacaatcaat catatgtatt tttgattgat ttaattcttc gattatattt ttactttttt
      841 cataaacttg ataaagctct ttcaacgtaa aataatagtt tttatctttt aatatttcat
      901 atatatcttt tatggataag gaaatatttc tatactgttt aagaataaga ccaacccctt
      961 ttcttcctat tccttccaga gataataatg agataatatc taaacaattt atgcttacca
     1021 ccacccatat tttcacaaat tgattaaaaa agaggctaga tatattttag cctctttttt
     1081 atcttttatt aagattcttg aatctttaaa tttggggagc aatactgacc aaatttcttc
     1141 cttgtcgctt ggataaatac atggcatcat ccgccgtttt tattaattcc tcaacattat
     1201 cagcaacttc tggataacca ctgacaccaa aacttgctgt aatcttggca ttttgaccat
     1261 tatatcgcaa ttcgtagttt tctattttct ttctgaattc ttcaatcact ttggctgcct
     1321 ttcttaaagg catatcagga agtataatga agaattcttc cccgccgtat ctcgcaataa
     1381 ttccatcata ttggtcaacc atctcctgac caaaacgggc aactgttttc agcacctcat
     1441 ctccaaataa atgtccatag gtgtcattaa attgcttaaa atggtcaata tcgaatatag
     1501 aaactgcaat aggtttttta ttcgctatag cttccccaac cagttgcggt aaaatgtttt
     1561 gcagatacaa tcggttatat atttttgtca aaccatcttt aattgcaata tcctccattt
     1621 gttcataaag tttagcattt tcaatagcca tagtaacttg catcgaaaca gctttgaaca
     1681 aatcggtact gctaacggaa aaataatttt ctaaagaatg ttcagccaat atcaaaccat
     1741 aagttttatt ggctttatat aagggtacag ccactaaaga gcctactttt cttcccttta
     1801 aaaattcata tttttcttca ctggaattgt tgtccattat aatctcttta ttcttaattt
     1861 tactatgtat aaaactgctc catacttgtt ctttaaactt ttcaataaca tcctggctat
     1921 aaatactttt aatgtgaaaa tctaaagaac cgtctttttc gttgtaaacg attatggaac
     1981 aggtatcaac ccccataatt cccataatca tatcacatac catttctaat aactgctcaa
     2041 tatcaagaat agagccaata taagaactga tttctcttaa aataaataac tcagcaacac
     2101 tatttctgaa attttcaact gtttttttca tttcttcttt ttgttcctga agttctttat
     2161 ttaagagctc aacttcatta ttcttcttat acaactcctt atttttttgc tggagttcat
     2221 tcaataattg tttgtctttt tctgcatctt ctttaaaatt agtcagtgtt gaatcaataa
     2281 cataaatgaa tatgattgaa attaaaataa tacaactaac attaattaaa atctcataat
     2341 taaagaattc ggtctttaat ttcaacaagc caaccataat tattgggaaa aaggaggata
     2401 taaaaacaga tttatgcctg aaatcctgca agatcatttc agccacaatt aataagtaaa
     2461 tcacggaagc aattaaggat attatttcat gataataaat taagtaactg ataacaagta
     2521 attctgcaat tttaaaagca aggtataaat attttttttc tagataacct ttttcaataa
     2581 aataagtttt tagtatcatt atcaatatac cagcaagaag aaagattaaa gcatcaccta
     2641 aatcagttaa aacacccgat atcttattga tttccagata aatgataatc agtaaaaaaa
     2701 ggtaaatatt aatgtttgta tacttttttt cagaaaactc aaatcttctc ataaaatatc
     2761 caccttattt gttttttccc aatatatttc tatgacatca ccattcttga ttgaatccgt
     2821 aaaagccgct ctgttaccat ttaattttag aataatattg ccttggggat gtgataaatc
     2881 aaaatcgata aaattaaaca catctacaaa aatataatcg gctttttcac tcttcataat
     2941 tactttttct ccattcactg tgacaatcac aggaatatcg gtttttttat tttcaagttc
     3001 atctgcctga gtcattttat cttcaaagac taacccttca ctggtatgat ctcttgtcac
     3061 tgtagttgga ttaacagcct tttgctctat ttcttcatta gaagctgtta aatgagacga
     3121 acgcatattg tagctaatcc cagctgtgtt tttattctct tctgcagata ttttaatctc
     3181 attattatct accacgtctt gagaaaaaga cttgttttct ataattttaa attctatgga
     3241 atccccgtct cttattatat aagaagaatc taccttgact ttattcacat agacctcttt
     3301 atcgtataaa ggaacatcca gataatctgc taaatcggct atggtattaa tgctgaggat
     3361 tttaacatcg tcattattaa atatttcatg gttcttatct actaattttc cattaatata
     3421 acatatggag gttaattcca tgtaggcgcc attaatcaat actcttttta gattcttagg
     3481 atcaataaaa tccctgataa aagcactggc atcttctcca ttgactgcag gctgtactaa
     3541 aatttcatcg ccgtcatgaa taattgtatc taaacttgct tcttttccat taacaaagag
     3601 ttccgcaggc tctgcaggtc cacctttaat ccgtcttctt tcaccgttta atttaaagaa
     3661 aagtgaagca ccttttcgtc ccagtaaatt ggtatgatcc aatccttttt gcatgcacac
     3721 atccaataca gtcaaacgtt ttgcatttaa aagctttact tcttccttgt taaaacgaac
     3781 tttaataaaa tcaaaacctc tttgcttaag ggcagtgaca catataccaa tgggtgtaat
     3841 gacatccggc gtatttaagg tttccgataa atatttaaca ttgattaaag agtccccacc
     3901 cctaactgct actctttcat tgggaatatt taatatttca gaaatttttt tggtcaatcc
     3961 tacaacctga cttcccccac ctacgcaaaa tactgcattg ggttttttat tcgcattcac
     4021 ttcacatact tttgctgata tttcttctgc taaacgagac acaggacctt ttactacttc
     4081 gtataattct tctgaagata ctgtatgact cacacccata atgtctgtaa aagtaatttc
     4141 gctttctttc ccaattttat atttaatttc ttctgcagta ttaaaatcta ctaaatattt
     4201 atgaacaatt tgctctgtta tttcatctcc ggcaatagga atcattccat aaccaataac
     4261 cgtaccatct tttgtaatgg ctatatccga tgttcctgct ccaatatcga ctaaagctaa
     4321 atttaataat cttagttttt caggtattgc cacattaatg gccgcaatcg gctccaatgt
     4381 cagatttgaa acaacaaggc ctactctgtc cattacagaa tataaactgt cgacaaccgc
     4441 cttaggtaaa aaggtagcta aaacatctgc cccaatgctt tttgcaatat gaccttccag
     4501 atttgaaata atatagtcat ccaagtaata atttacaaca gaataaccta ccgaataata
     4561 ctctatttca tcatccttat agtcttcttt aattttttgc tgagcttctg atattccttt
     4621 taattccaac tcccttataa gatctctttg aatttctttt tcttcctcaa aatgaatttc
     4681 tgcttttact aaataagttt ttaaaagccg tcctgcagcc gcaatagaaa catccgtaag
     4741 ttctattcct gttctttctt ctaaaatatt ttttaccttt ataactgcat tggctacttt
     4801 agaaatatca tgtatttgtc cgtctatcat agctcttgac tcatgtgcca caacagcact
     4861 gtcaacaaca acaaaattgt catcttcctg gtagcctatc acgcccatga cctttctggt
     4921 ccctatatct aatccaaaaa caagatcttc ttcctttatg ttataattta atatcacttt
     4981 ctactccccc attttttaat ttggatgagc tacaaagatg gaaaaattct cttatatata
     5041 atgataacat atgtaacaaa aaaatctata ataaatttta ttcttagaca aaaaaataca
     5101 acatttttaa aaattaaatg tataatgaaa aaattagaaa agaacaaagc aagccctatt
     5161 ggccgctttg ttcatttttt tccggctcat aaataaacca gtccttgata ttaccaatat
     5221 aaaagtctct ctgtggctta agttctccct gtatcctgtc atgggtaata agagcagcat
     5281 tcctgaaata aagactgata tatggtaatt catcagcaat atatttctgt aattcatcat
     5341 aggccatttt catattttct tctccaacgg ctgtaaaagc cttttcaagt aaactgtcca
     5401 tctgttcact tctaaaagaa ataaaattac tgccattttc tatttgtgaa gaatgaaagg
     5461 caaaagtaaa gtcaggaatt ggagaaagtt tccatcctcc aataaatgca tcaaaattct
     5521 tttcctgtaa acgtattaaa aactcttctt tggaaacctc ttcaagattt accttaatac
     5581 ccagttcgct taacatttta ataatttccc tggctgcctc ttttcttgtt tcatcttctg
     5641 attctaccaa taaattaaaa ataaaatcga ttttacttcc attcatcgtc ttatccaata
     5701 caccatcatt gtctgaatcg ctccagccac tttctgctaa aagttccttg gctttttcta
     5761 tatcgtaatt atatttttca atttcatcac aataaagcca ggaagttggg cttattggta
     5821 catcggttaa aacaccatga tttaaatatt gattctccaa aatattttgt ttatcaatgg
     5881 cataagctac ggcttttctt gccttcttat cctgaaataa aaccttgtta aagttaaaac
     5941 caatgaaatc ataatatgaa gttacatatt ccttaaccct gctttctcca ctttgagcat
     6001 actttcccca atccacagca tttgtaccaa ttaaatcaat ttgtccctgt tcaaatgaat
     6061 ataactctgt ttcttcatcc ggaataacaa gaactttaat tctttctata taaggttctc
     6121 ccttaaacca ggcaggattc acttctaact gtaactcttt cgctacttta tattctccta
     6181 atttatatgc ccctgtccct acaggcactt tctccaacaa agcttgtcca ctggaagttt
     6241 cataaatatg agccgggata actggaaagt acaaagcata aactgcccca ctaaaaggct
     6301 ggttgtaaaa tattttaacc gtataatccc cttcagcttt gtaagaaaca atattttcaa
     6361 tacatttctt atatggagat gtactgggtg cattttttat aacgtctaaa gaaaaaatga
     6421 catcccgggc agtaaaagga tctccgtcat gccactggac accctgccgg agattgattg
     6481 taatagtcgt tccgtcattt gaaaaagtcc aatccgtagc caagtttgct atgggttttt
     6541 gagactcatc aaaatctatc agggtatcaa aaaccaattt taatacctga tcgactgtat
     6601 aatcctcgtt taataaagga tttaacgttt ttggcatcct aaaggaaata atcatttctc
     6661 cacctttagt gggctcaggc agttcaacgg tttgctcttc ttctgtttcc tgagggtcaa
     6721 tagaattatt atcttcgttg tatgtacagc ttgtcatatt caatattaaa aaaaatgcaa
     6781 gcattaagga ttttaatttt ctactcaact taccgcacct tcctaagtat tattattgta
     6841 gtttctgatt attgtaaatg gatttataaa gttttttata aattctcctg ctatcaagca
     6901 ccttttttac ataagctttt gtttctaaaa agggaatgtt gtggaccttt ttaccatcct
     6961 tgctataatt tttgtcctgc agccattttg aaacatttcc gctgccagca ttataagctg
     7021 ccagactggt ttcaatatcc tcaaattgat ccagtaattt cttaatatac caacaaccaa
     7081 tgtgaatgtt gatttccggg tcaaacagtt tttcactgct atattcttct atccctattt
     7141 ctgcagcagc ccattcaccg gttttttcag taatctgcat aaggcccttt gcatttttat
     7201 gggattgtgc atttggatca aatttgcttt caacttttat tattgaaaaa accaaataag
     7261 ggtctaaatc atataactct gaatatttta aaactaaatg atcatacttg agcgggtaaa
     7321 aaataatctt aatcaattct aaacctgcat aaaaaaagaa aatgaaaagc ataagaaaaa
     7381 ataaaaaacg gaacttcttt tttcgaaggt caataatata aaacataatt gaactccttt
     7441 atcataattt atccactgga aataatgtga tttaactgtt tcatggtata ggcttcatta
     7501 tgcccattat caataatgac atctccatat tttttcattt cttcccatgg catttgagag
     7561 gcaattcttc gttttgcttc ttctgtagtc attccatctc tttgtgcaat tctttttaat
     7621 ctgatttctt ccggagcata aactaaccat atctcatcta ctgctttatg aagtcccgat
     7681 tctattaaaa gtgcagcatc aacaatgata tatttacagg ctttatcact gctttttatt
     7741 tgatgaatcc gttcaatgat ttcctgaata ataaggggat gggttatctt gtttaaaatt
     7801 tcaagcaaag attggttggc aaaaacaatt tgccccagtt ttttacggtc aatttctcca
     7861 tccgccttta ggatagcttc tccaaatctc tcaagtacag catcatatgc aggtttaccc
     7921 ttttttaata tttcatgtcc aattttatct gcatcaataa tacagacaga tgtcagttgg
     7981 gataataata cagctactgt agtttttccg ctaccgctgc cacctgtaag accaataacc
     8041 ttcattttgt cctcctttat ttagcctcaa accaagtatc cccaaaatga atgtcaactt
     8101 caagaggaac atctaatttt actgcatttt ccatttcatt ttttagaatt tcttttactt
     8161 cttccagttc atctttatga acttcaacta aaagctcatc atgtacctga aggattaggc
     8221 gggatcttaa attcctttcc tttaacttct tataaaccct taccattgca attttaataa
     8281 tatcagctgc tgttccctga ataggtgtat tcattgctac tctttctcca aaagaacgca
     8341 gattaaaatt ttttgagcta atttcaggaa taggtcttct tcgtccgaat aaagtggtta
     8401 cataacctga ttcttttgca ttttcaaccg ttctattcat gtattccttc acttttggat
     8461 atttttcaaa ataactctca atatattcct cggcttcttt tctgctaata tttaaatcct
     8521 ggcttaaact aaaagctcct attccataga tgataccaaa attcacagct ttagcatttg
     8581 acctttgtaa ggaagtaact tcttcaacag gaaccttaaa aacttttgaa gccgtaattt
     8641 tatgaatatc tgccccacta cggaatgcat cgattaaagt ctcatcctgg gcaacatgag
     8701 ctaataccct taactcaatt tgagaataat ctccgtctaa taacagatat tctttgcttt
     8761 gaggaataaa tacttttctg attttccgtc ccatttccag tttgatagga atgttttgca
     8821 aattaggttc tgtactgctt atacgaccag ttgccgtaat ggtctgatga aaagtagaat
     8881 gcaatttacc tgtttcttta ttgactgcag catacaaacc atccccgtaa gtcgtcttca
     8941 gttttactaa ttgacggtat tctaaaattt gattaataat tggatgttta tttttcaatt
     9001 tttccaatac ttctgctgca gttgaataac ccgtttttgt ctttttttct acaggaagca
     9061 tcaatttttc aaaaagaata cttcctaatt gtttaggaga atttatatta aatttttctc
     9121 cagctaaatt atatatatta ttcgtaagaa tatcaatttt ttccgttaat tcttctatat
     9181 attcttttaa ccggttaata tcaattttaa atccgtgttt ttccatactg aacaatactt
     9241 caataagtgg gagctctatt tcataataaa gaaattcctg attattttct tttatttttt
     9301 cttccatgat ttttgaagca tgaaaaacaa tacaggcttg ttcagaagta atatcggtta
     9361 ttttattttc ttctaaatcc gaaagtaatt ttttattttt acctttaccc agaagttctt
     9421 cttcgcttgg gaaaatttct cctaaataat catgagctaa atcatcaaag ccataactat
     9481 cttttatggg attaagaatg taagccccca gcatggtgtc aaaagaaaga ttatttaaag
     9541 atatgccaaa ccggcttaaa atatgcatgg tacttttcgc atcgtgtgtt atttttttta
     9601 ttttccttga ttcaaaaagt tcttttgttt tgttcataat ttcttctatg ggtagagttg
     9661 gggaagcttc aatccagatc ccatcagaac cgtcatagca gcagcttatc cccagtatct
     9721 tatgattttc tttaaaaata agaaaagcaa actcaccttt caaaagaata tcttttatga
     9781 cttcagttaa ttcattgatt ccatctactt ttttatgatt tctgtttttt gaaggattgg
     9841 ctgccctcgc caccgttgtt ttaaatctgt caaaaaaact cttaaattcc aattgcttaa
     9901 accattcgta aactttggga ttcattaact catgcatttt taaactttcc cagttgattt
     9961 caatgggcac atcggtgcat atggttgcca ggtatttact ttgtcttgct tgttcttcat
    10021 aaacacgtaa attttctgaa acacgagcag gtttaatttt ttcactatta gcaatggcat
    10081 tttcaatatt gtgatattcg tgaataattt tataagccgt tttttctcca atacctggta
    10141 ccccaggaat attgtcagaa gaatctccca ttaaagcctt tacctcaata tactcctgag
    10201 ccgttacacc aaacttgtct attaaatcct tttcataata atcttctatc tcagttttgc
    10261 tacccctggt ttttgggatt ctaatctttg taatgtctga ggccaattgt aaaagatccc
    10321 tgtcgcctga taccacaata ggactcatac catttttttc tgcagccttt gctaatgttc
    10381 ccaatatatc gtctgcttca tatccttcca tctctaaaat cgtaatgccc attgcctcta
    10441 aaagctgctt taatatagga atttgaacca ctaattcatc aggcatccct tttctgtttc
    10501 ccttatactc tttaaatact ttatgcctga aagttggtgc ctttaaatca aaagcaaccg
    10561 ctatgtaatc gggctggtct tcttctatta atttaaatag aatatttaaa aagccatata
    10621 tcccattggt ataagtaccc tgggaattgg ttaacaaagg aactccataa aaagccctgt
    10681 taacaatgct atttccatca aacagcataa ttttattcat tggtcttctc ctttttattt
    10741 tattgaactt taatatttaa tgtccttctt gaagtaaggc tatactttcc ttttccaccg
    10801 taaaaaatca cataaccttc ttcatagata gaaattactt tagcagagga attccaatta
    10861 taaacctttt gcagttcttt ttcttttact ctggcattat tcgtaaacac aatcaaatca
    10921 ggttttacag catttaagaa atcttcttct aatacttcta ttcttttgtt gccattaata
    10981 gtaataatat cggagctaat ctttgaagaa ctgagcatta aatcactcat atccattttt
    11041 tccatatctg gcagtagcaa aatagatttc ttcggcaaaa ttaactttac tatgattttt
    11101 ctttcactaa caggtattgt cgtatctgat gaaggcctta aaaacaataa tttagcttct
    11161 cctactgacc attcttcccc gctgttgatt tgataaatgg atatcccttg ttcttttgcc
    11221 ttatataaaa actgttcggt ttctttatct aaatccgata tatgtggaag atatattttt
    11281 tcgattgtat aatcatctaa cagactttca aaaccctgta ttttatcttt cttgggagaa
    11341 gcaataatca gttcagaaat agtatttacc ttttgactat ttaaatactc ctttatatgt
    11401 ttggtatcct tttctgagac ctctcctatt aatatgtttc tttctttaat ttttatcaat
    11461 gtgcagtggc cgataggaat ttccataaaa tgtatttctg tctcatagct ttttttagat
    11521 gaatgattcc aaaaaatcaa aaatatcaat ataataatta tgattgcaga taaaatatat
    11581 aaaatattcc attttttcat cctatcacct ccgtttttgt attataacat aaaaagttcc
    11641 gaatagaaat tcggaacttg taataaaata atttttttca gtaaaaactt ttatattgct
    11701 aacaaacatt ttagtactga taaatcactt ccacaatggc atttatatcc atttcaccta
    11761 tttcaactgg tgtagaaaca gcatttgttt taaaactttc ttcgtccgca tacacaaccg
    11821 accttccacc ataactggtt tctacaactg aaaccggagc ttttataacc acacctaaag
    11881 cctctccaat tgctgctgcc tttccctggg catttttaat ggcttgtttt aaagcttcat
    11941 tatagtattt ttctgtatct gatacactaa attgtatgcc tcctgacaaa ttagctccct
    12001 cttttactgc taaatctaag agttctccta cttttgaaat atccctgacg gtaacagaaa
    12061 ttgtattggt aaccgtataa cctacaattc tctctccagt cttagattca taatcgtatt
    12121 gtggataaac agaataacca cttgtttgta tgtctttttc atcgataccc atatttttca
    12181 ctgcagcgat aacttgattc attttatttg cattttcttt ttgtgcttgc tttgcatctt
    12241 tgttttctgt acgcactcct aatgtgatat aagccatatc tggttttact ttaatgaccc
    12301 cttctccacg gacagttatt ttgtttatgc ctgtctcggc tttaaccaca gcaggactgc
    12361 ttaaacttgc cgttgtaaaa aagacagcta ataataagac cgataaaagc tttattgttt
    12421 ttttcattat tcatctctcc ttttgtgtat catcttaata atttttttaa atattggtct
    12481 tgcaatcata aaagtaatcc ctaaaatcac tatcaatagc aataaaggta tcagggcata
    12541 ggataatgaa accacaaaat cttcaaaagt ttgccgtaca tcattgatgg atcggttaaa
    12601 gtttgattta acccttgcca tcaaattggg gtcagccatt tttatgggct ctatggtttt
    12661 tacttctttt atggtaattg taaaggtaga aaattcaaca agcttatccc agttttttat
    12721 catgctttta tagatttcta aattcgttct gacttgatta agtctttctt ctaatttgat
    12781 taaatcttca acttcttcag ccttttccat gatttctaac aaacgctctt gttctaattc
    12841 cagcattctg attctgcttt cggtttctat atactgctct gtaatatttt ctgtcatttc
    12901 gttctgacta ataagatgtc caaattttac aagttcattg gaaacatccc catatttctc
    12961 aaccggaatc cggatgacga tagtaccttc ttttaaataa atatctcttt ctggctcaga
    13021 ataataaata tagctcgaaa agttctcaac ataacctcct gatttttgtg ttaattctct
    13081 gattgctttg actgtgtcat caaataattc tacttccagg gataaataag cattatatat
    13141 tatttttctt tctccggcat ccacagaaac tgcattaaaa gtgtttccag cctgattgat
    13201 tgactcttct tccagacttt ccatactgta atccgcagcc ataccgccat agtctgaagc
    13261 catatcggtt tttgctaatt cagcgctact tttaaaagaa tcactggaag ctatttcatt
    13321 tttactatat gattgttttg ctgcttcaaa ttctccggct acaccgtttt gttcagattt
    13381 cattcccata tttccaatgg acgtcatgat aaaaaatagc aaaagaaaac tcgcagcaac
    13441 cgctgtcaac ttcttaagat taaaatctaa ttttttcttt tctgatttct tttcatccct
    13501 ttctttatat aattttaaca ttaaatcttt atgataatcc tcaggtaagg ttttttccgg
    13561 tatcttttgt atttcttcaa ttaaaaactg aatttcttcg agttcttgtc tgcattcttt
    13621 acaagtcttt aaatgatttt caaaggctat tttttcttca ttctctaaca caccgtcgat
    13681 atagaaagaa atccattccc tgtactgttc acaaatcatt agttaccctc ctttccagtc
    13741 tttagacggt ttttaaagtc aaaatgtttc tcttttataa gcacgttttt taattctgtt
    13801 cttgccctgg atattctgga ttttactgtt cccaaagaac atcctaatat ttgagagatt
    13861 tgcttatagt caaagccctg aaaatctcta aggataatca cttctttata agtttccgat
    13921 aagtgattta aggcccattg aatttgtgaa gccagttctt tattcatgag ctcttcctca
    13981 ggatttaatt gcttatcttg aaattccctg gggattgtcc cttcatccgt ttgaatgtct
    14041 ctatcaatag aatagctttc tcttcctttt cgttttctca attcatcaat acatgtattc
    14101 attgcaatac gataaagcca ggtagaaaaa ctggagtctt ctctgaattt atgtaaactg
    14161 ttaaaagctt ttataaaaac ttcctgggat aaatcttttg catcttcctc gttatttaga
    14221 atccggtaag caatattata tactttactt tcatgctcta taattaactc ttcaaaggcc
    14281 gatatatttc ctttttttgc tcttttaaca agtaactttt cttctttccc ctccattttt
    14341 tctctccttt ctatatataa taatcatcaa taaaatcata ctatatcttt tgacgtttta
    14401 cgaatataat ttgttccatt tctaacgctt caataattta tttcttgatt tttaaatttt
    14461 aatatgataa gattgttagg caaaagcttc tgcggatgtg gcggaattgg cagacgcagc
    14521 agactcaaaa tctgccggtg gcgacatcgt gggggttcga gtcccttcat ccgcatgaaa
    14581 aatacataaa aaactcagca tttgctgagt tttttatgtt tatttatata cattttttct
    14641 gtaagattta ggtcttatcc attgcttctt cttttctaat tccatataag cataatatgc
    14701 ttttttcatt atttgccgtt catcattaaa tttaagcaaa tgataagctt catgaaattt
    14761 ataaaatccc gcatcaacaa agtattcttc cttctgaggc ttacaataaa aactgttcgc
    14821 ccgcattaaa taccaatgaa cagttttatt aacaatatcg tcctttccct gaaaagtata
    14881 ctggctatga cctatgtatc gtataacttt tgcagccaca gatgtttcct catatacttc
    14941 ccttaatgct gtctgcctgt acatttctcc gggttctacc gttcccttgg gaagtaccca
    15001 gccaacatac ttatgcttat aattcttgta taataacaag attttccctc tatgaatcac
    15061 aacacccccg cagcttactg cttctatcat aataaattcc tcctgacgaa taaaaacttc
    15121 atgcagaaag ggcacacaaa tggcacacca aaacgcatcg ttaattctta ctactatttt
    15181 taattatttt aattataaca taaaaaatta aattacttat aacatgaaaa agtatacatc
    15241 tttttttgat atttttcaac cttgagattg caaaagaaaa atgccatttg tattttttta
    15301 tcaattttta tatttaaaag gagattgttg tatatgttct ttcatttttc gcattccctg
    15361 aatccacaga tataacacaa aaggaacgaa taaaatcatt aa
//
LOCUS       DAOSUD010000050        15387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2351_length_15387_cov_23.497978, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000050 DAOSUD010000000
VERSION     DAOSUD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_15387_cov_23.497978"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1186)
                     /gene="spoVAD"
                     /locus_tag="PLG49_07335"
     CDS             complement(170..1186)
                     /gene="spoVAD"
                     /locus_tag="PLG49_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOQ17169.1"
                     /translation="MAKKLGNQTIKFDLPPSIVSTASTVGPKEGKGPLSSYFDVVLHD
                     PLWGEDSWEKAESKMVKKTIERVIEKGNLTPEDIDYIFAGDLLNQLTASTFGIRGLAV
                     PFFGLFGACSTMAEAITLGAMAIDGGFANYIVAEASSHFCSSEKQFRFPLELGTQRPL
                     TASWTVTGSGAVILGKFGPSPYVTYATTGKIVDLGIKDPMNMGAAMAPAAADTIITHF
                     EDTGFLPSDYDLIITGDLGSVGRELLLELLRKHNYDIENIYTDCGIEIFDSVTQDTHA
                     GGSGCGCCAVTLAGYLYKEMKNNKWNRILFVPTGALMSPGSTQQGESIPGIAHAVTIE
                     NNWA"
     gene            complement(1197..1625)
                     /gene="spoVAC"
                     /locus_tag="PLG49_07340"
     CDS             complement(1197..1625)
                     /gene="spoVAC"
                     /locus_tag="PLG49_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOQ17170.1"
                     /translation="MKKEYSQMVTRVSPKSNLLRDCARAFWVGGLICTIGQLITNIIK
                     SFGYNQDEASMLTTIILVFLGILLTGLDIYDSIGKYAGAGSIVPITGFANSIVSPAIE
                     YKKEGYIFGVGAKMFTIAGPVLVYGTIASVIVGIIHYLFK"
     gene            complement(1659..2090)
                     /locus_tag="PLG49_07345"
     CDS             complement(1659..2090)
                     /locus_tag="PLG49_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HOQ17171.1"
                     /translation="MFWGYLFNVLAGLAGGIVISGGVFAFIAIIGIITRLIDKTKTVK
                     FILLYETVIMLGGILGTFTMVWDLSFPIGKIGSAIIGFSFGIFVGCLAVSLAEILDVI
                     PIIARRLFIKKGIQFFLLSVAIGKMIGSLIYWIIPGFVQYK"
     gene            complement(2093..2707)
                     /locus_tag="PLG49_07350"
     CDS             complement(2093..2707)
                     /locus_tag="PLG49_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HOQ17172.1"
                     /translation="MQIYIKPYKNTKIYKKKKILLSDIAEIAPANLKDISLEDILILE
                     IKEDKKQNYHISIIDIIKAICEKYPDVNIVNVGEKDIMIEYLPDDKKENPLITFLKVV
                     SVCLILFAGGGIAIMTFHTDSALPDVLIKLNEIFTGTKTDRPYWIEIPYSIGIAVGII
                     VFFNHFSSKKITDDPTPIEVEMDSYADSIEKCIINSFIEKKRGN"
     gene            complement(2682..2870)
                     /locus_tag="PLG49_07355"
     CDS             complement(2682..2870)
                     /locus_tag="PLG49_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17173.1"
                     /translation="MLNKNTLGGILFTIILLMTVFFAICYYFFFSNEKLPSRGEYVEN
                     IIELEYGGNQGANLYQTI"
     gene            complement(3036..3755)
                     /gene="sigF"
                     /locus_tag="PLG49_07360"
     CDS             complement(3036..3755)
                     /gene="sigF"
                     /locus_tag="PLG49_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HOQ17174.1"
                     /translation="MDKTIELIKKAQAGDTAARDKIVEENIGLVWSIVKRFKNRGYEI
                     DDLFQIGSIGLIKSIDKFDVNYDVKFSTYAVPMIMGEIKRFMRDDGMIKVSRSLKETA
                     AKAKAAKEKLTKDLEREPSIQELADEIGVEVEELTMALEANCDVESLQSVIFEGEGNN
                     PITLIDRGDFDYHQEAKMIDKIALNQIIEKLKPKEKQIILMRYFKDKTQTEIADMIGV
                     SQVQVSRIEKKILKSMREMFG"
     gene            complement(3773..4204)
                     /gene="spoIIAB"
                     /locus_tag="PLG49_07365"
     CDS             complement(3773..4204)
                     /gene="spoIIAB"
                     /locus_tag="PLG49_07365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOQ17175.1"
                     /translation="MEFQNSMKISFLSKSQNEAFARVAVAAFVSQLDPTVEEISEIKT
                     AVSEAVTNAIIHGYEDKEGEVLLTCTIQDNLVEITVSDRGVGIEDIEKAREPLYTSKP
                     ELERSGMGFTVMEAFMDEVEVESVKDSGTTVKMRKRLKSLE"
     gene            complement(4219..4560)
                     /gene="spoIIAA"
                     /locus_tag="PLG49_07370"
     CDS             complement(4219..4560)
                     /gene="spoIIAA"
                     /locus_tag="PLG49_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HOQ17176.1"
                     /translation="MNFSCSVRTDSLIVKIKGEIDHHSCDFIREKLEREYARHNVKNI
                     IFDFSDIHFMDSSGIGVIMGRYKSVNERGGKLGVYNVNPQIDRVFQLSGLYKNKIVHS
                     FKSLEEAITNI"
     gene            complement(4714..6081)
                     /locus_tag="PLG49_07375"
     CDS             complement(4714..6081)
                     /locus_tag="PLG49_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ17177.1"
                     /translation="MICVGCQREIQDNLIKCPYCEFDIASYKKIKRLSAQFYNKGLEQ
                     AKNRELSNAIESLNTAIGLDKTNIDARNLLGLIYYEIGEIGMALTHWVISSNLKKEDN
                     LAINYMDKIQKNTMQLEAYNDSIKMFNQSLEYIKQKSEDLAIIQLKKALQLNPHFVKA
                     HCLLAACYMSEKEQDKALNHIEKALQIDVSSSNAIRYLKMLKPNFSVASLSRTAERRP
                     ATVNLKSKNISRSSSPQMGQLLGFIAGAVCTFAVLFTLVIPDKTNDLKKRISELDSER
                     QRLEERINETIEEKNEIITALREENKSLQESNNQLQQKQLAMEQEEKINQAESLYGSR
                     KYEEAAELLYSIDEAFLSPETKIKYENLKNNVFPQAARIHYDRGRSSYSKGNYENARA
                     DFEKSLLYKQDANYSDNALYYIARTYEHEQDLEKAKELYSQVIERYNGTDGAANANRQ
                     LVNLE"
     gene            complement(6087..6242)
                     /locus_tag="PLG49_07380"
     CDS             complement(6087..6242)
                     /locus_tag="PLG49_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17178.1"
                     /translation="MINFEEELNKYQASVEVDNIEEVIQSNGIRDIIDVLTSIVKKYE
                     EKQNNKE"
     gene            complement(6244..7542)
                     /locus_tag="PLG49_07385"
     CDS             complement(6244..7542)
                     /locus_tag="PLG49_07385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOQ17179.1"
                     /translation="MNYDAVTSYLVQIERFGSRPGLERIIKLLNFLGNPEKNLNVIHV
                     AGTNGKGSVCSMLQSVLMNAGYQVGMYTSPHLERYNERLKINNQDISNDDFTDIGEKV
                     IWAAKKCVENGTEHPTVFECLTAMAFLYFYEKKVDFVVLEVGLGGRHDATNVIKKPLV
                     SVITAIGKDHQDVLGNDIESIAYEKGGIIKKNCATVLYFPNRLVYNVINEICQKENSP
                     LFYNSNIKFKDVRHSLDKMFFSIETDLYTYDGLELSLLGEHQLYNAATVLTTVEILKK
                     QGVVIPESSIRQGLKDCYWPGRIEIIKKEPLVILDGSHNEESAQALSQFLKDYLNNKK
                     ITLLIGILKDKPYKKIIDTILPFAKKVIITEPDSPRKLSASELAELVKAYPVEFIKCP
                     DIKEAVELAEKLLSEEDVLVCTGSLYLIGKVKSYLSKRGR"
     gene            complement(7647..10307)
                     /locus_tag="PLG49_07390"
     CDS             complement(7647..10307)
                     /locus_tag="PLG49_07390"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOQ17180.1"
                     /translation="MKKNLEKVYDPSKVEDRLYQKWLEKKYFHAEVDYSKKPFTIVIP
                     PPNITGTLHMGHALDNTLQDILIRWKRMQGYNALWMPGTDHASIATEVKIVQKMAEEG
                     LTKADLGREGFLERAWKWKEQYGGTILKQLQKLGSSCDWDRERFTMDEGLSKAVLEVF
                     VRLYEKGWIYRGAKIINWCPECQTTISDAEVEYEEKAGHFWHIRYPLKDGSGYIQIAT
                     TRPETMLGDTAVAVHPEDERYKDYIGKTVILPLVNREIPIIADEYVDKEFGTGAVKIT
                     PAHDPNDFEVGLRHNLPQINILNDDGTLNEEVGKYAGMDRYEARKLIVKDLEEQGFLV
                     KIEDHVHNVGLHDRCSTVIEPMIKMQWFVKMEELAKPAIEVLKKGDLRFVPERFGKVY
                     LNWLENIRDWCISRQLWWGHRIPAYYCNDCGNIDISREAPKVCSKCGSSSLRQDEDTL
                     DTWFSSALWPFSTLGWPDKTPELEYFYPTDVLVTGYDIIFFWVVRMVFSALEQMGEVP
                     FRDVLIHGLVRDSQGRKMSKSLGNGVDPLEIIDKYGADALRLTLVTGNAPGNDMRFYY
                     ERVEASRNFANKIWNAARFILMNFEDEDEIHVELKDLLAADKWILSKVNTLAKEVTEN
                     MDKYELGIAVSKLYDFFWEEFCDWYIEMVKPRLYNKEDKTRKAALWTLRTVLINALKL
                     LHPYMPFITEEIFTYIQDEEETIMLSAWPQYKEEWNFFVEENEIELMKQAIRNIRNIR
                     AEMNVPPAKKAKVYVVSDDVKVQDIFERGRTFFSPLAYASELVIQKDKAGISNDAVST
                     VIPNATIFIPFEDLLDIQKEIERLENEKKRLEKEIERVNNKLNNKGFVEKAPQKVVEE
                     EREKGIKYETMYKQVTERLEALKSNKKQDD"
     misc_binding    complement(10445..10687)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10739..11905)
                     /locus_tag="PLG49_07395"
     CDS             complement(10739..11905)
                     /locus_tag="PLG49_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17181.1"
                     /translation="MGELAAKTKKAFKSVWRFIRLGFFTKKRPEDYDLFEFDDDVKKA
                     NSLCEELLDTGKKKILLLKELEELTKKEKELEKFKALTEEDIKRIRKLLFDHKAITDE
                     MEALKGRLISRNRILSTIAHYEDNFPDIIDEIREAEEKQSRIKRDMDYLIGEKEALIY
                     SREQLEMAHGFLEKFSKGLVVLFSLVAVIFAYITLSKDSSIFVPLSLTAIGVMLVGTM
                     TYFFKRRVVYELSKNGLMQAKVVKLLNGAKIRYVYYTNYLDYEYNKYNVESVEQLQRS
                     WELYKKNKHHEKRYNNINIRMQQIEEEVLSLLKSKDIYVDYFEDIKRWSRIEERRNVL
                     NSIQKEKNIVEAKLDYLDKYQEEIWAQLEELKEKDETEDKIIEKVISERLEELS"
     gene            complement(11991..12272)
                     /locus_tag="PLG49_07400"
     CDS             complement(11991..12272)
                     /locus_tag="PLG49_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HOQ17182.1"
                     /translation="MRTEEILIRNCTAEHLPQPYNLYAELIGVDKLYILAKELGGTTI
                     YIPKTQYLLKEVMEVQLKKEFDGGNYKELAQKYNVCEKTIRNWLKKKKS"
     gene            12532..12903
                     /locus_tag="PLG49_07405"
     CDS             12532..12903
                     /locus_tag="PLG49_07405"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOQ17183.1"
                     /translation="MKKMNEMIKNLVRKYDTTNPIELANHLGIIVKLNPYSEKTKGLY
                     VKILDIPFIIINSNLKKEDQNFLLAHILGHILLHFNQDTIFAWENSVFLQGIQENEAN
                     EFALRLIAENQTNPINYQLTF"
     gene            complement(12872..13069)
                     /locus_tag="PLG49_07410"
     CDS             complement(12872..13069)
                     /locus_tag="PLG49_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOQ17184.1"
                     /translation="MKVIIDRFEEDYAIVELENKKMISMPKELIPKGAKEGMVINISI
                     DLDETKKREEILKKLADSLWD"
     gene            complement(13079..14413)
                     /locus_tag="PLG49_07415"
     CDS             complement(13079..14413)
                     /locus_tag="PLG49_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ17185.1"
                     /translation="MKKLNPTFKFKNISLYLFIVFTAFVLIGCTGANQKSSEEFIGVS
                     EKPDSVNIFNQKTASVLGEEKEEEIVEENQALTQELRVHFIDLGQADSVLIQQGNEFM
                     LIDGGNNEDSELLVSYLKEQGVNHIKYLIATHPHEDHIGGLDAVINAFNIEKLIMPNI
                     THNTRTFEDLLLAVQKKNLKITAPKVGDSYELGEGNWTILAPYPKEYEDLNNYSVVIS
                     YAYGSHTFLFTGDAEKEAEFDMLKENVANPEIFKADVLKVGHHGSSSSTSSEFLDAVS
                     PSFAVISVGAQNDYNHPHEETIKRLQERNIEILRTDLNGNIIFFSDGKELTYITGKKA
                     DVTKKTTIDKLPERAIIISHLDKKEETVTVQNCSNQDLDLSDWYLLSVTGNQKFIFPD
                     GYILKAASSVVIASGDSKGDLHWTAKNVWNNSKSDPAELYDNMGNLIYRWND"
     gene            complement(14609..15295)
                     /locus_tag="PLG49_07420"
     CDS             complement(14609..15295)
                     /locus_tag="PLG49_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOQ17186.1"
                     /translation="MNLIYKGKTKNVYQLEDGNYLLEFKDDVTGEDGVFDPGANSVGL
                     TIEGAGKSCLRITKMFFELLKEKNIPTHYIDADIDASTMKVLPAKTFGNGLEVICRFR
                     AVGSFYRRYGMYVKQGQPLDALVEFTLKDDARQDPPITEDALAMLGILTKDECQTIKE
                     LTRKIAEIVKEELKKKGLELYDIKFEFGRHKETGEIILIDEISGGNMRAYKDNEYIEP
                     LTLEKILLDE"
BASE COUNT         4741 a         3224 c         2022 g         5400 t
ORIGIN      
        1 atcgattaaa atttgaccaa aaacacaaat ggcgcctcct actataaaag ctttaatata
       61 ttccatatca tacctcctct ggtatctgat tcagttaaat gattatcctt aagcagaaac
      121 ttttaaacat tgtgaagtgt tttattaaat acgccaaaaa taattcagtt taagcccaat
      181 tgttttctat cgttacagca tgtgcaatac ctggtataga ttctccctgc tgggtgctgc
      241 ccgggctcat aagtgcacct gttggaacaa ataatattct gttccattta ttattcttca
      301 tttccttata taagtaacct gccaaagtga ctgcacaaca gccgcaacca ctaccacctg
      361 catgggtatc ctgagtaaca gaatcaaaaa tctcaatacc acaatccgta taaatgtttt
      421 caatatcata attatgtttt cttaaaagct ctaacagcaa ttctcttccg acactgccca
      481 aatcccctgt gataattaaa tcataatctg aaggcaaaaa acctgtatct tcaaagtgcg
      541 taataatggt gtccgctgct gcaggtgcca tagcggctcc catattcatc ggatctttta
      601 tccctaaatc cacaattttt cctgtagtcg cataagtcac atagggactg ggaccaaatt
      661 taccaagaat aaccgctccg cttcccgtta cagtccatga agccgttaaa ggacgttggg
      721 ttcctaactc aagcggaaat ctgaactgct tttctgaact gcaaaaatgg ctggaagctt
      781 cggctacgat ataattagca aaaccaccat caatagccat agcgcccaag gttattgcct
      841 ctgccatagt ggaacaggca ccaaaaagac caaaaaaggg aacagccaaa ccccttatac
      901 caaaagtact ggcagttaat tgattgagta aatcacctgc aaaaatataa tcaatatctt
      961 ccggggttaa atttcccttt tctattactc tttcaatggt tttcttaacc attttacttt
     1021 cagccttttc ccaactatcc tctccccata aaggatcatg taaaaccaca tcaaaataac
     1081 tggataatgg tccctttcct tctttaggtc ctacagtaga tgctgtagaa acaatacttg
     1141 gaggtaagtc aaatttaatc gtctgattac ctaatttttt tgccatacta tcctccttac
     1201 ttaaacaaat aatgaataat accaacgata acggatgcaa ttgtaccata aacaaggaca
     1261 ggtcctgcta ttgtaaacat cttggcacca acaccaaaaa tatacccttc ttttttatat
     1321 tctatggctg gtgaaacaat ggaattggca aagccagtaa taggtacgat agaacctgca
     1381 cctgcatatt ttccaataga atcgtatata tcaagacctg tcaaaaggat acctaaaaag
     1441 actaggataa tcgtcgtcag catactagct tcatcctggt tatagccaaa actttttatt
     1501 atattggtaa taagctgtcc aatggtacat ataaggccac caacccaaaa agccctggca
     1561 caatccctta acaaattaga cttgggggaa actcttgtaa ccatttgaga atattctttt
     1621 ttcatattct gaatctgatt catcattcat cctccttttc acttgtattg cacaaatcct
     1681 ggtataatcc agtaaattaa agaacctatc atttttccta tagctacact taaaagaaag
     1741 aactggatgc cttttttaat gaataacctt ctggcaataa ttggtataac atctaaaatt
     1801 tctgctaaag aaacggccaa acagcctaca aatatcccaa aagaaaaacc tatgatggct
     1861 gaaccaattt tcccaatagg aaaagataag tcccatacca tcgtaaatgt tcccaggata
     1921 ccgccaagca taatgaccgt ctcatataaa aggataaatt tgactgtttt ggtcttatct
     1981 atcaatctgg taataatacc gatgattgct ataaaggcaa aaacccctcc tgaaatcaca
     2041 atgcctcctg ccagtccggc caagacatta aataaataac cccaaaacat tattaatttc
     2101 ctctcttctt ttcaataaat gaattaatga tacatttttc tatactgtca gcataagaat
     2161 ccatttcaac ttctatgggc gttgggtcat cggttatctt tttagaactg aaatgattaa
     2221 aaaaaacaat aatcccaact gctattccaa tagaatacgg aatttcaatc cagtaaggcc
     2281 tgtctgtttt cgttccggta aaaatctcat taagttttat caatacatcc ggaagggctg
     2341 aatctgtatg gaaggtcata attgcaatgc ctccaccggc aaataaaata aggcatacac
     2401 taacgacctt taaaaaagtt ataagggggt tttccttttt atcatcgggt aaatactcaa
     2461 tcataatatc tttttctcca acattgacaa tattaacatc aggatatttt tcgcaaatgg
     2521 ccttaataat atcaataatt gaaatatgat aattttgttt tttgtcttcc ttgatttcta
     2581 atatcaaaat atcttctaag gatatgtctt tcaaatttgc aggagcaatt tctgcaatat
     2641 ctgaaagtaa aatttttttc tttttataaa ttttagtatt tttatatggt ttgatataaa
     2701 tttgcacctt gattaccccc atattccaac tctatgatat tttctacata ttctcccctg
     2761 gatggaagtt tttcatttga aaaaaagaaa taataacata tggcaaaaaa gactgtcata
     2821 agaagtatta ttgtaaacag aattcctccc agagtgtttt tattaagcat cttaccctcc
     2881 tttttatctt taaatcataa tcctgaaagc attatttttt aaatcctcta ctataaatta
     2941 actttcctct aatctgtttt tttatactat ttttttattt ttaaaagaca attctttata
     3001 tacttataaa aagtacaaaa aaaagaaaat gccccctaac cgaacatttc cctcatagac
     3061 tttaatattt tcttttcaat tcttgaaacc tgaacttgag atacaccaat catatctgca
     3121 atctctgtct gggttttatc tttaaaatat ctcattaaaa taatttgctt ttctttaggt
     3181 ttaagttttt caattatttg atttaaagcg atcttatcaa tcatttttgc ttcctgatga
     3241 taatcaaaat ctcctctgtc aattaaagta atagggttat tcccctctcc ttcaaaaata
     3301 actgactgaa gcgattctac atcacaattg gcttctaaag ccatggtcag ttcttctact
     3361 tcaaccccta tttcatcggc taattcctgt atagaaggtt ctctttctaa atcctttgtc
     3421 agtttttctt tagccgcttt agcctttgca gcagtttctt ttaaagaacg actgacttta
     3481 atcataccgt catcccgcat aaaacgctta atttccccca taatcattgg aacggcataa
     3541 gttgaaaatt ttacatcata attgacatca aacttatcta tacttttgat aagtcctata
     3601 cttcctatct gaaacaaatc atctatttca taccctctgt ttttaaaacg tttaacaata
     3661 ctccaaacca aacctatatt ttcttcaacg attttgtctc tggcagctgt atccccggcc
     3721 tgtgccttct taatcaactc tattgtttta tccatctgcc cacctctttt agctattcta
     3781 gacttttaag tctcttacgc atcttaacag tcgttcctga atctttaacc gattcaactt
     3841 caacttcatc cataaaagcc tccatcactg taaaacccat gccagaccgt tccagttccg
     3901 gttttgaggt atataaaggt tctcttgcct tttcaatatc ttctatgcct acccccctgt
     3961 ccgaaaccgt aatttcaacc aggttatcct gaatcgtgca agttaaaaga acctctcctt
     4021 ctttatcttc ataaccatga ataattgcat tggttacagc ctctgaaaca gccgttttaa
     4081 tttcagatat ttcctcaact gtaggatcta actgagaaac aaaagctgct acggcaaccc
     4141 tggcaaaagc ctcattttgg gatttgctta aaaaagatat cttcattgag ttttgaaatt
     4201 ccatgacgat tccccctact agatatttgt tattgcttct tccaagcttt tgaatgaatg
     4261 aacaatttta tttttatata gaccagaaag ctgaaaaact ctgtcaatct gcggattaac
     4321 attatatact ccaagttttc cgcccctttc attgacactt ttatatctgc ccataataac
     4381 accaattccg gaactatcca taaaatgaat atccgaaaag tcaaagataa tattttttac
     4441 attatgcctt gcatactctc tttctagttt ttccctgatg aaatcgcaag aatgatggtc
     4501 aatttctcct ttaattttta cgattaaact atccgttctt actgaacatg aaaaattcat
     4561 cctactcacc ctttttcatt tcttctttct tttttgcatg atagaaaaat aaagataaat
     4621 gcctgaataa atttccaaac aattaatata ttctccataa aatggtttat tcctttaaaa
     4681 taaaaaaaat aataccaaaa acctggtatt attttattca agatttacta gttgtctgtt
     4741 tgcattggcg gcaccatcag taccattata acgctcaatg acttgactat acaattcttt
     4801 agccttttcc aaatcttgct catgttcata agtcctggca atataatata aagcattatc
     4861 agaatagttc gcatcctgtt tatacagaag ggatttctca aaatctgccc ttgcattttc
     4921 ataattacct ttggaatatg aacttctgcc tctgtcataa tgaattcttg ctgcctgtgg
     4981 aaaaacatta ttttttaaat tttcatattt aatctttgtt tccggggaaa gaaaagcttc
     5041 atcaatggaa tacaaaagtt cagcagcctc ttcatatttt ctgcttccat acaggctttc
     5101 tgcctgatta attttttcct cctgttccat agctaactgc ttttgctgca gttgattatt
     5161 gctttcttgt aaactcttat tttcttctct taaagccgta atgatttcat ttttttcttc
     5221 aatggtttca ttaattcttt cttctaatct ttgtcgctca ctatccaact cgctaattcg
     5281 cttcttgaga tcattggttt tgtctggaat taccagagta aaaagaacag caaatgtaca
     5341 aactgctcct gctataaatc ctaataattg tcccatctga ggagaactgc ttcttgaaat
     5401 atttttactt ttaagattaa cggttgctgg tcttctttca gcagtccttg ataaagaagc
     5461 gactgaaaaa ttaggcttta acatttttaa atacctaatg gcattagaac ttgaaacgtc
     5521 aatttgtaaa gctttttcta tatgatttaa ggccttatcc tgttcttttt cactcatata
     5581 acaggcggct agaaggcaat gcgctttcac aaaatgagga ttcagctgga gggctttttt
     5641 caactggata atcgctaaat cttcactttt ttgcttaata tattccaatg actgattaaa
     5701 catcttaatc gaatcattgt aagcttccaa ctgcattgta tttttttgaa ttttatccat
     5761 atagtttatg gcaagattgt cttctttttt taaattggag ctaatcaccc agtgggttaa
     5821 agccatgcca atttcaccaa tttcataata aatcagaccc aaaagattcc ttgcatctat
     5881 attggtttta tccagaccaa tggctgtatt aagggattca atggcattac tcaattccct
     5941 gttttttgcc tgctctaaac ctttattata aaactgtgca gaaagacgtt tgatcttttt
     6001 ataagaagct atatcaaatt cacaataagg acattttatg agattgtctt gtatttccct
     6061 ctgacatcct acgcaaatca cggtatctac tccttattat tttgcttttc ttcgtatttt
     6121 ttaacgatac tggttaatac gtcgataata tccctgattc cgttcgattg tatcacttct
     6181 tctatattgt ctacttctac agaagcttga tatttattta attcttcctc gaaattaatc
     6241 atattaccta ccccttttag acaaataaga tttcactttt ccaatcaaat ataaggaacc
     6301 tgtacaaact aaaacatctt cttctgacaa taatttttct gctaattcga ctgcttcttt
     6361 aatatccggg cattttataa attcaaccgg ataagcctta accagctctg ccagttcgct
     6421 ggctgacagc ttacgtggac tatccggctc tgtaataatc acttttttgg caaagggtaa
     6481 aattgtatca ataatttttt tataaggttt atcttttaag ataccaatca gtaaagtaat
     6541 ctttttattg tttaaataat cttttaaaaa ttgggaaagg gcctgggcac tttcctcgtt
     6601 atgactgcca tccaggataa ctaagggctc tttttttata atttcaatcc ttcctggcca
     6661 gtaacagtct ttcaaacctt gtctgatact actttcagga ataaccacgc cctgcttttt
     6721 tagtatttct actgtagtca ggactgtggc agcattataa agctgatgtt ctcctaaaag
     6781 agaaagctct aatccatcgt aagtatataa atcggtttca atggaaaaaa acattttatc
     6841 cagactatgt ctaacatcct taaattttat attggaatta taaaataagg gtgaattttc
     6901 cttttgacaa atttcattaa taacattata caccagtcta tttgggaaat acaatacagt
     6961 cgcacaattt ttcttaatta ttccaccttt ctcataagca atggactcga tgtcatttcc
     7021 taaaacatcc tgatgatctt ttccaatagc agtaatgact gacaccagcg gttttttaat
     7081 cacatttgta gcatcgtgcc ttccgcctaa acctacttcc aatacaacga aatccacctt
     7141 tttttcataa aaatacaaaa aagccatggc tgttaaacat tcaaatacgg ttggatgttc
     7201 tgtgccattt tctacacatt tttttgctgc ccaaataacc ttttcgccaa tatcagtaaa
     7261 gtcatcattg ctaatatcct gattatttat ttttaatcgt tcattatatc tttccaaatg
     7321 aggagaagta tacatcccca cctgatatcc tgcattcatc aaaacagact gcagcataga
     7381 acataccgag cctttcccat tggtacctgc tacatgaatg acgttcaaat ttttttcagg
     7441 atttcctaaa aaatttaaca attttattat cctttccagt cctggtcgtg aaccaaaacg
     7501 ttcaatctgt accagataac tggttactgc atcataattc aacgaaatct cccgctttcc
     7561 atccataaag tatgctgtca caaaaataag taaatatatt atatattata ttattagtat
     7621 aaaaatatat aaataaaaaa caagacttaa tcgtcttgtt ttttattact tttaagtgct
     7681 tcaagtcttt ccgtcacttg cttatacata gtttcatatt taataccttt ttctctttct
     7741 tcttctacta ctttttgagg cgctttttct acaaagcctt tattatttaa cttattatta
     7801 accctctcaa tttctttttc caggcgtttc ttttcatttt caagtctttc aatttctttt
     7861 tggatgtcta gcaaatcttc aaacggaata aaaatggtgg cgttgggtat cactgtggat
     7921 acagcatcgt tactgatacc agctttgtct ttttgaatga caagctcact ggcataagca
     7981 agaggactaa agaaagttct tcctctttca aagatatcct ggacttttac atcatcagaa
     8041 accacataaa cttttgcctt tttagcaggc ggtacattca tttcagccct aatatttcta
     8101 atatttctaa tagcttgttt catcaattca atttcatttt cttcaacaaa gaaattccat
     8161 tcttctttat attgaggcca ggctgaaagc ataatggttt cttcttcatc ctgaatataa
     8221 gtaaagattt cttctgtaat aaaaggcata taaggatgaa gcagttttaa ggcattaatc
     8281 agaacggttc taagcgtcca taaagctgct tttcttgttt tatcttcctt gttataaaga
     8341 cggggtttta ccatttcaat ataccagtcg caaaattctt cccagaagaa atcatacaat
     8401 tttgaaacag ctatacccaa ctcatactta tccatatttt cagtaacttc tttagccaga
     8461 gtgtttactt tagataaaat ccatttatct gctgcaagta aatctttaag ttcaacatga
     8521 atttcatctt catcttcaaa attcattaat ataaatcttg cagcattcca tatcttgttg
     8581 gcaaaatttc tgctggcttc tactctttca tagtagaaac gcatatcatt acccggtgca
     8641 tttccagtga ctaatgttaa acgaagggca tcggcaccat acttatcaat aatttctaaa
     8701 ggatctacgc catttcctag agatttgctc atctttcgcc cctgagaatc tctaaccagt
     8761 ccatggatta atacatctct aaaaggcact tctcccattt gctccaaagc tgaaaatacc
     8821 atgcgtacaa cccagaagaa aataatatca taacctgtca ccaatacatc cgtaggataa
     8881 aaatattcca attcaggagt tttatctggc catcctaatg ttgaaaaagg ccatagggca
     8941 gaactgaacc atgtgtccag ggtatcttca tcttgtctta aggaactgct gccacattta
     9001 gaacatactt ttggtgcttc acgacttata tcaatattgc cacagtcatt gcaataataa
     9061 gcaggaatcc tgtggcccca ccataattgt cttgaaatac accagtccct gatattttca
     9121 agccagttca gataaacttt accaaagcgt tcaggaacaa atcttaagtc gcctttcttt
     9181 aaaacctcaa tagctggttt tgccaactct tccattttta caaaccactg cattttaatc
     9241 attggttcaa tcactgtact gcatcggtcg tgaagaccta cattgtgtac atgatcttca
     9301 atcttaacca aaaagccttg ttcttctaaa tctttcacaa tcagctttct tgcttcatag
     9361 cggtccatgc ctgcatactt tccaacttct tcatttaatg taccatcatc atttaaaata
     9421 ttaatttgag gcagattatg tcttaaacct acttcaaagt catttggatc atgggcaggt
     9481 gtaattttaa ctgctcctgt tccaaactcc ttgtcaacgt actcatccgc aatgatgggg
     9541 atttctctgt tcactaaagg taaaataact gtttttccta tataatcttt atatctttca
     9601 tcttcaggat gtacagctac agctgtatcg cccaacatgg tttccgggcg ggtggtggca
     9661 atttgaatat agccactgcc atcttttaaa ggatagcgga tatgccagaa atgacctgct
     9721 ttttcttcat actcaacctc cgcatccgaa atcgtggtct gacattcagg acaccagtta
     9781 ataatctttg ctcctctata aatccagcct ttttcatata aacggacaaa cacctccaaa
     9841 actgcttttg aaagaccttc atccatggta aagcgttccc tgtcccagtc acaagagctt
     9901 ccaagctttt gaagctgctt taatatcgta ccaccatatt gttctttcca cttccatgcc
     9961 cgttcaagaa aaccttctct gccaagatcg gccttagtta aaccttcttc agccatcttt
    10021 tgaacgatct taacttcagt ggcaatactg gcatggtcag tacctggcat ccaaagcgcg
    10081 ttataaccct gcattctttt ccagcggatt aaaatatcct gcagggtatt atcaagcgca
    10141 tgccccatat gcaaagtacc cgtaatattg ggtggcggaa tgacaatcgt aaaaggtttt
    10201 tttgaataat ccacttcagc atgaaaatac tttttctcta accatttttg atacaatcta
    10261 tcctctacct tagaaggatc atatactttt tctaaattct ttttcatact gatttccttt
    10321 ctatcataat tttatctata gtgattgtaa aaaataataa aaccaagcaa acacacttac
    10381 taaagtcata acccctcccc tctttatctt acttttccta tcattaaaaa atctctttca
    10441 gcccataaag gacgaaagag attccgtggt accaccttca tttcttttta tattatttta
    10501 tacactcaaa ataatataaa aagacactct actgacgata acgagtcaaa ccgtttgtat
    10561 ctactaaact ttcaatacaa aagctccgaa gctaccttct atggcatatt ccctagaaat
    10621 cttccagcca aaggatctcc tctctgtaag ggtatgacca tatactcctc ttcttcatcg
    10681 cttatcacaa tttatttgtt taattgtata aaagccttta atatttgtca aggatttgtt
    10741 aacttaactc ttctaagcgt tcactgataa ctttttcaat aattttatct tccgtctcat
    10801 ctttttcttt tagctcttct aactgagccc agatttcttc ctgatactta tccaaataat
    10861 ccaatttagc ttccacaata tttttttctt tttgaatgct atttaggaca tttcttcttt
    10921 cttctattct gctccagcgt ttaatatctt cgaaataatc tacataaata tccttacttt
    10981 ttaaaaggct caggacttcc tcttcgattt gctgcattcg gatattaata ttattgtatc
    11041 ttttttcatg atgcttgttt ttcttataaa gctcccagct tctttgtaat tgttctacac
    11101 tttccacatt atatttatta tattcataat ctaaataatt cgtataataa acatagcgga
    11161 tttttgctcc atttaagagt tttaccactt ttgcctgcat taagccgttt ttactaagtt
    11221 cataaacaac ccttctttta aaaaaataag tcattgtacc taccagcatg acccctattg
    11281 ctgtaagaga caaaggaaca aaaatactgg aatccttact taaagtaata taggcaaaaa
    11341 tgacagctac cagactaaat aaaacaacca gaccttttga aaacttttct aaaaatccat
    11401 gagccatttc cagttgttct ctactgtata taagcgcttc tttttcacca attaaataat
    11461 ccatatctct tttaatcctg ctttgctttt cttctgcctc tctgatttca tcaatgatat
    11521 ccggaaagtt atcttcataa tgggcaatgg tacttaaaat cctgttccga ctgattaatc
    11581 tgcccttaag ggcttccatt tcatctgtaa tggctttatg atcaaataaa agcttgcgga
    11641 ttctttttat gtcttcttct gttaatgcct taaatttttc taattccttt tcttttttag
    11701 ttaattcttc aagttcctta agcagaagga ttttcttttt cccagtatcc aataattctt
    11761 cacaaaggga attggctttt ttgacatcat catcgaattc aaacaggtca tagtcttccg
    11821 gccttttctt cgtaaaaaag cctaaacgga taaatctcca aacagactta aaagctttct
    11881 tcgtttttgc tgctaattcc cccatcaata acacctacaa tcataaataa agattattct
    11941 acattttttt attataccac atttttagcc ggaataacat tttatttatt tcagcttttt
    12001 ttctttttta accaatttct tatggtcttt tcacaaacat tatatttttg tgccaattcc
    12061 ttataatttc ccccgtcaaa ttccttttta agctggactt ccatcacttc tttcaaaagg
    12121 tattgcgttt ttggaatata aatagtcgtt ccccctagtt ctttcgccag aatatataac
    12181 ttgtccacac caataagctc cgcatataaa ttatacggtt gcggtaaatg ttccgcagta
    12241 cagtttctta taagtatttc ttctgtcctc attttccctc tcccttcttt gaattctaag
    12301 acttatgcct gtttcctgta tgtatacttt tattatagtt tctatattgt atactgtcaa
    12361 ccataatttt atttttttta caaaaataat aaaaattaca tatatttgta tttatttttt
    12421 ctaaagtatg atataataga gtgggtgata gggctaagag aacacaagat gattatagta
    12481 tggctacttt atggcgtgag gatctttgtg tgaaaagtag gagtgattca aatgaagaaa
    12541 atgaatgaaa tgattaagaa tctcgtccgc aaatatgaca caacgaatcc cattgaattg
    12601 gctaatcacc tgggcattat tgttaagctt aatccttact ctgaaaaaac gaaaggcctc
    12661 tacgtaaaaa tattggatat tccctttatc ataattaaca gcaacttaaa aaaagaggac
    12721 caaaactttc ttttggccca cattcttgga catattcttc tgcattttaa tcaggataca
    12781 atatttgcct gggaaaactc cgtgtttctt caaggtatac aagaaaatga agcaaatgaa
    12841 tttgccttaa gattgatagc ggaaaatcaa actaatccca taaactatca gctaactttt
    12901 taagtatctc ttctctcttc ttagtttcat ccaggtcaat agatatattg ataaccatac
    12961 cctcttttgc acctttaggt ataagttctt taggcatact aatcatcttt ttgttctcta
    13021 attccactat agcatagtct tcttcaaaac gatcaataat aactttcata tgtcacccct
    13081 aatcattcca tcgataaatt aaatttccca tattatcgta caattcagca ggatccgatt
    13141 ttgaattatt ccagacattt tttgcagtcc aatgtaaatc gcctttactg tctccactcg
    13201 ctataacaac actggaagca gcttttaaaa tatagccatc agggaaaata aatttttgat
    13261 tacccgttac cgacagcaaa taccaatctg acaaatccag atcctgattg gaacaatttt
    13321 gcacggtaac ggtttcttct tttttatcta aatgagaaat aataattgcc ctttccggta
    13381 gcttatcgat cgttgtcttt tttgtcacat ctgctttttt acctgttata taagttaatt
    13441 cttttccatc agaaaaaaat ataatattac catttaaatc tgttcttaaa atttcaatat
    13501 ttctttcctg caaacgttta atcgtctctt catgcggatg attgtagtcg ttttgcgctc
    13561 caacactgat aactgcaaaa cttgggctta ctgcatctaa aaattctgat gaagtcgaac
    13621 tgctgcttcc atgatgtccg acttttaaaa catctgcctt aaatatttca ggatttgcaa
    13681 cattttcttt tagcatatca aattcagctt ccttttcagc atctcctgta aataaaaaag
    13741 tatgacttcc atatgcatag gaaataacaa cagaataatt attcaaatct tcgtattcct
    13801 taggataagg cgctaaaatg gtccagtttc cttccccaag ctcatagctg tcacctactt
    13861 taggggctgt aattttcaaa tttttcttct gaactgccag caaaagatct tcgaaggtcc
    13921 ttgtattatg agtaatatta ggcataatca acttttcgat attaaatgca ttaatcactg
    13981 catccaatcc gccaatatga tcttcatggg gatgggtagc aattaaatat ttaatatgat
    14041 tcaccccttg ttccttaagg taacttacca ataattcact atcctcatta ttgccgccgt
    14101 ctatgagcat aaattcattt ccctgctgta tcaggacaga atctgcctga cccagatcaa
    14161 taaaatgaac tctaagctcc tgcgttaacg cctgattctc ttctactatc tcttcttcct
    14221 tctcttctcc caaaacagaa gctgtctttt ggttaaaaat attaacagaa tcaggttttt
    14281 ctgaaacccc aataaactct tctgaactct tctgatttgc acccgtacag cctataagaa
    14341 caaaggcagt aaaaacaatg aacaaatata aagatatatt tttaaattta aaggttggat
    14401 tgagcttttt catcatagac tcctctctga aaaatccttt cataaacaaa ataatataaa
    14461 tctaaaacta ttatataaca aaaagttgta aaatactaca caaaacaagg aataaaaaga
    14521 tttagtgaca aattaataaa tttccacaca aaaaaagtga gattccaaga atctcactca
    14581 cattgctaaa aacagcccat atattgactt attcatctaa aagaatcttt tctaaagtca
    14641 gaggttcaat atattcatta tctttatacg ccctcatatt gccaccagat atttcatcaa
    14701 tcagaatgat ttctcccgtt tctttatgtc ttccaaattc aaatttaata tcataaagct
    14761 caagaccttt tttctttaat tcctctttaa cgatttctgc tatttttctg gttaattctt
    14821 ttatggtctg gcactcatct tttgttaaaa tacctaacat agccaatgca tcttccgtaa
    14881 taggaggatc ctgacgggca tcatctttta gcgtaaactc tactaatgca tccagaggct
    14941 gtccttgttt aacatacatt ccataacgtc tgtaaaagct tccaaccgct ctgaagcggc
    15001 aaataacttc taatccattt ccaaaagttt tggctggcag tactttcatg gtggaagcat
    15061 caatgtctgc atcaatatag tgagtaggaa tatttttttc ttttaataat tcaaaaaaca
    15121 tttttgtaat tcttaaacaa gatttccctg caccttctat ggttaaacca acagagttag
    15181 ctccaggatc aaaaacccca tcctcacccg ttacatcatc tttaaattct aataaataat
    15241 taccatcctc aagctgatat acattttttg ttttaccctt gtaaatcaaa ttcattttat
    15301 cactcctcat tttggaaata agctatataa atataaaaat aaaaaaacat cgggtaaact
    15361 gtaaaataaa cgatttaaaa gaattaa
//
LOCUS       DAOSUD010000051        15076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2404_length_15076_cov_25.273950, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000051 DAOSUD010000000
VERSION     DAOSUD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_15076_cov_25.273950"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLG49_07425"
     CDS             <1..1007
                     /locus_tag="PLG49_07425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ17187.1"
                     /translation="GIHTKEGKNLYFIDKKKTFLNDFDESSKITYDKLGKIKPIFWLF
                     CYIITFLLFLVPEIFFSIVIQFFMIVTHTEEKLMPILQNPYGSMTGQWVVVSVTGVHI
                     LAFFIYIKCIERRPFKSIGFKGNEKIFKYLKGACIAVLMQLIYWGIILIFGFGKIAPE
                     AVHMTNALGLSAIGPVLIFLIAFLIQGASEEIAVRGWLLPVLARHYKPVIAIIFSSLF
                     FGFMHASNPNVAVLPLINLVLYGIFAALYALYDDGLWGICAQHSIWNWFMGNVLGLPV
                     SGMFLGQSSIIETSLTGPEWITGGGFGPEGGIIVTFIFTVASLILIKKLIHKGIIVKN
                     "
     gene            complement(1083..2369)
                     /locus_tag="PLG49_07430"
     CDS             complement(1083..2369)
                     /locus_tag="PLG49_07430"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOQ17188.1"
                     /translation="MSKENYNAYKNAQAQFDSVAKKIGLDEAICDLLRQPLREYHFSI
                     PVKMDDGTSKVFRGYRIQHNDARGPAKGGIRFHPDETVDTIRALSMWMTWKCAVVDIP
                     LGGGKGGVVCDPRVLSEREQEQICRGYVRQLARNVGPIQDVPAPDVMTNAKHMLWMLD
                     EYEAIHGAKYPGFITGKPVGMGGSLGRTEATGYGVIFVLREALKELAIDITKTTAAIQ
                     GFGNVAQYAAELYTKLGGKVIAVSCWDNNDKTAYTYKKLDGIDVDVLMSITDRFGCID
                     KDKARELGYEILPGDAWLEQDVDILIPAALENQITAENVNKISSQVKVIAEAANGPTT
                     PDADKVLNERKVFVIPDFLANAGGVTCSYFEQVQCNMNYYWEKDEVMQKLDTKMTAAF
                     KAVYDLAVNKEFNMRDAAYVISINRVAEAVKKRGWL"
     gene            complement(2513..3397)
                     /locus_tag="PLG49_07435"
     CDS             complement(2513..3397)
                     /locus_tag="PLG49_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ17189.1"
                     /translation="MVTKLDLYKVFCEVANCKSFSKAAKNLYMTQPAVSQAIMSLEKE
                     LGVRLFTRTSKGVALTNEGQLLFEYTNSAINLINVGEKKLFEARNLMAGEMKIGVGDT
                     ISRYFLLPYLSVFHDNSPNIKLKIINRTTIELCTLLKSGEIDLAICNLPVKDSAIAVK
                     KCMDIHDIFVCGEKYKELVGKKVSLSQLLNYPLIFLERKSNSRQYVEKFFTAKGLKLT
                     PEIELGSHELLLEFSKYNFGIACVIEEFSKDYLDSKLLYKINLTEEIPKRAIGFCFLK
                     SVSLSPAAQKFVDIIGYQ"
     gene            3498..4688
                     /locus_tag="PLG49_07440"
     CDS             3498..4688
                     /locus_tag="PLG49_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOQ17190.1"
                     /translation="MTRITGTIARGLRTPIINKGDNLVEIVVDTVLKASQVENFSIRD
                     RDIIGITESVVARSQGNYASIDDIAKDIKAKFGDDTIGIIFPILSRNRFAICLRGIAK
                     GAKKIVLMLSYPADEVGNHLVDIDLLDEKGINPWTDVLTEKDFRNYFGYQKHPFTGID
                     YIEYYKSLVEEYKVECEIIFSNNPKTILDYTKSVLVCDVHTRQRTKRILKANGAEKVF
                     GLDDILTKPIDGSGYNEHYGLLGSNKATEETIKLFPNRCQEIVEQIQAIIKEKTGKTV
                     EVLIYGDGAFKDPVGKIWELADPVVSPGYTKGLEGTPNEVKLKYLADNNFAGLKGEEL
                     EKAISDYIKNKKENLVASMESQGTTPRRLTDLIGSLCDLTSGSGDKGTPVIYIQGYFD
                     NYAK"
     gene            complement(4744..5418)
                     /locus_tag="PLG49_07445"
     CDS             complement(4744..5418)
                     /locus_tag="PLG49_07445"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ17191.1"
                     /translation="MVHIGNDWDELLKDEFKKDYYLRLRQFLAKEYRTKTIYPSMYNI
                     FEALKLTSYRDTKVLILGQDPYHGENQAHGLAFSVQKGVDIPPSLINIFKELKDDLGC
                     YIPNNGYLVPWAKQGVLLLNACLTVEANKANSHRNKGWEFFTDYIIQLLNEKETPMVF
                     ILWGNNAKEKIKYINNPKHLILTAAHPSPLSASRGFFGCRHFSKANEFLRKNNQTEID
                     WQIPNI"
     gene            complement(5437..6411)
                     /locus_tag="PLG49_07450"
     CDS             complement(5437..6411)
                     /locus_tag="PLG49_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOQ17192.1"
                     /translation="MNFFDLPVEELKVYKPNQTKAEDFDLFWDSIIKESKAQPLNTEY
                     IAMDYPVEQIKVYDVYYDGFRNSRIHGRLILPAAASKDNKVPVVVMYHGYNWNNRVIS
                     HTFKYLLLGYGVFIAEVRGQNVTSPDHNHYDNGGASGWMTLGIYNHKNYYYLYVYMDC
                     ARIIDLLMQREDIDTNRIAVEGGSQGGALSLAVAALCPEVKVVMADCPFLCHFKRAVE
                     LAQEYPYLEISHFFKIHDSLHKTEEQVYKTLSYFDNMNLASRIKGEVLMSVGLEDAIC
                     PPSTCFAAFNHIQSNKEMRIYPDYGHGGFSQQEEERLIFLKKRFDKEY"
     gene            complement(6490..7770)
                     /locus_tag="PLG49_07455"
     CDS             complement(6490..7770)
                     /locus_tag="PLG49_07455"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOQ17193.1"
                     /translation="MGIKEMALEAKQASIKLAVASAELKNKALEEIIKGLENHKNQII
                     KANQEDLERSKNENLAMPLLKRLKFDEGKIEDVIQGIKSLIALDDPVNVTQAITELDD
                     GLELYRVSCPIGVIGIIFESRPDALVQISTLCLKSGNAVLLKGGSEAKNTNRILYEII
                     KEACRKANMPEGWIQLLESRDDVNEMLKMDKYINLIIPRGSNEFVRYIMDHSNIPVLG
                     HADGICHCYVDADADINMAVDIVEDSKTQYVAVCNAAETLLVHQAVAEEFLPKLKEKL
                     EKKQVELYGCEKTRKIIDILPASEDDWKTEYLDYKLSIKLVSDIGEAIDHINQYGSGH
                     TDAIITNNKENADLFMNLVDSGNVFLNCSTRFSDGFRYGFGAEVGISTNKIHARGPVG
                     LDGLVIYKYKLFGNGHIVADYANKVKKFKHKKIK"
     gene            7955..9013
                     /gene="hisC"
                     /locus_tag="PLG49_07460"
     CDS             7955..9013
                     /gene="hisC"
                     /locus_tag="PLG49_07460"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ17194.1"
                     /translation="MSKYWNRLTKSLVPYVPGEQPKDKKYIKLNTNENPYPPSPNVIE
                     AIKYAVNEDLKLYPDPNADALRKVIADYHGININQVFVGNGSDEILSFAFAAFFDSSE
                     KILFPDITYSFYPVYADFYNIQYQEIPLKEDFSLPIEQFNIPNGGIVLANPNAPSSKY
                     VPLEDLEIILKNNLEKVVIVDEAYIDFGGESAVKLINQYPNLLVIQTLSKSRSLAGLR
                     VGIAMGTDELIEGLNRIKNSFNSYTLDRLAIAGAIAAYKDKEYFEKTRKKIIETREKT
                     MKELRKLGFTMPDSMANFIFVSHPKVNAEEIFLALRERGILVRYFKKPRISNYLRISI
                     GTDEEMEILINNLKEIIK"
     gene            complement(9069..9923)
                     /locus_tag="PLG49_07465"
     CDS             complement(9069..9923)
                     /locus_tag="PLG49_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ17195.1"
                     /translation="MVQIIADSTCDLSQELLEKYNIKILPLMINLEGDSYKDGVEITP
                     DEIYQWSDKVGKTPKTACPSLGDAIDFLTPFTQKGDDIIFFGISEAMSATCGVIRLAA
                     ENLDYEKIWVIDSKNLSTGIGLQVLRAAEMAMEGASADDILKEMDKIIPKVRASFVID
                     TLTYLYRGGRCSAVTSLLANTLKLKPKIIVEDGKMDVGKKYRGNIKSVILKYAKDLEE
                     DLLKADKRRIFITHSGCSEEIIKEVYDYLKGLNYFEEILITRAGGVISSHCGPNTLGV
                     LFIAGDEA"
     gene            complement(9941..10318)
                     /locus_tag="PLG49_07470"
     CDS             complement(9941..10318)
                     /locus_tag="PLG49_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17196.1"
                     /translation="MFFIGIFGIQTKEKEITAIQNTICPSCEALGSYQIIKTYDYFHI
                     FFIPIFKWNIKYYIRTSCCRKAFMIDSQLGKEIEEGKIVDLRNEDLSGFNTITEKGKC
                     LYCGAFVDDDRYAYCPYCGKPLI"
     gene            10422..11108
                     /gene="sfsA"
                     /locus_tag="PLG49_07475"
     CDS             10422..11108
                     /gene="sfsA"
                     /locus_tag="PLG49_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOQ17197.1"
                     /translation="MQLNYNTVYGTFIQRPNRFIAYIDVDHQKISCHVPNTGRLKELL
                     YPGAQVILSHHPSGKRKTDYSLRMIKKKNHWISIDSQLPNALAYEAILNGNIEELRGY
                     SMVKREICFGKSRFDLQLSGNDTCFVEVKGVTLEKDNWSYFPDAPTQRGRKHIDEMIG
                     VIEKGYRGVILFIVQIPYAKGFSPNWATDPGFAQKLKEASEKGVEILAYKCHISPNEV
                     FVTEKIPVIL"
     gene            complement(11213..12175)
                     /gene="argC"
                     /locus_tag="PLG49_07480"
     CDS             complement(11213..12175)
                     /gene="argC"
                     /locus_tag="PLG49_07480"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOQ17198.1"
                     /translation="MKKYKVFVDGQTGTTGLQIHERLKKHPFVELLTIDYDKRKDTEI
                     KKKFLNEADIVFLCLPDDAARESAKLVSNPTTKIIDPSVAHRVADGWTYGMPELSKEQ
                     REKIRESKRVTVPGCHASAFILAVAPLVKMGLMPSDYPVTAHSITGYSGGGKSLIEKY
                     ENEEINAPYIKAPRPYGLNLNHKHLPEMKVYTGLDRYPVFLPVVSNYYKGLAVSVPIH
                     NHLLNKKAGAKDIAELLSEYYKDECFVKVMPYNPMDLLFDGAFDITACNDTNQCEIFV
                     FGNDEKEMSLIFARLDNLGKGASGAAVQNMNIVLGLPEETGLES"
     gene            complement(12294..12932)
                     /locus_tag="PLG49_07485"
     CDS             complement(12294..12932)
                     /locus_tag="PLG49_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOQ17199.1"
                     /translation="MEAFIFDMDGVIIDSQPIHFEVELAGLRSFGIQKGADFLEKYAG
                     MTDYEMWELIKKEYPIHETTEKILEEQIKEKIRKVRETDISPIEGIEKLIKNLCEKKI
                     PIAVASSSPKLLIKEILNKLALYDYFDIVLSGEEVKKGKPAPDIYLETAKLLKVNPEA
                     CVVLEDSRNGVMAAKAAGMKCIGYINPNSGRQDLSKADIVVKNICEIDIRFL"
     gene            complement(12944..13138)
                     /locus_tag="PLG49_07490"
     CDS             complement(12944..13138)
                     /locus_tag="PLG49_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HOQ17200.1"
                     /translation="MSQYVPLGDNYILLSWLNMKLRNECKNLEELCERYNLKEEEVLE
                     KINSIGYIYDKDTNKFIANE"
     gene            complement(13285..15018)
                     /locus_tag="PLG49_07495"
     CDS             complement(13285..15018)
                     /locus_tag="PLG49_07495"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOQ17201.1"
                     /translation="MDKLLKKIEEKIVMILLVTVYFFTLILLVFLSSHPMFSQLTAYN
                     LNPIVINGIITQLQLLILVFLVLYFNNKKGYSIALLLNAINFILMLFSLFKRNSDGVI
                     AGIVTALGIFLILTVINNCLVEGERRKKQLYQLAYFDSVTGITNRNVIIHKVRKLTSN
                     LSKNKGKFALVFLDIDNFTAVNDTAGHLVGDLLLKKIAERIKEKIHKEDLFGRTDGDE
                     FAIIIQRDLSKEEILEYLNEIRRELTDVFFIEDKEFLLTASFGVALYPEDGPTASDIL
                     KHADISMQHAKKNGKNSISFFSNDIKEKLLTRMKLENELKKAISNKELYLVYQPQYYS
                     YKNEIRGFEVLLRWNSKELGPISPSVFIPIAEENNSIIEIGHWVLNEAMRQFKNNILK
                     YNDNFKLSINISSVQLTDNMFISKVERALKENDFNPKNLEFEITESVFISSEKCAERV
                     INYFKNLGITIALDDFGTGYSALSFLQYNHMNILKIDKAFVDKILITDPNKSLVELII
                     SMAHKLGMEVVAEGVEEKKQVEYLMKHNCDYFQGYLFARPLAIDSVLDLLNDMHRGRQ
                     DIKYLYEYNLE"
BASE COUNT         4876 a         2818 c         2202 g         5180 t
ORIGIN      
        1 aaggcattca taccaaggag ggaaaaaatt tgtatttcat tgataaaaag aaaacctttt
       61 taaatgattt tgacgaaagc tcaaaaatta cttatgataa acttggcaaa attaaaccaa
      121 tattttggtt gttctgctat atcattactt tcttactttt cctggttcca gaaatattct
      181 ttagtattgt tattcaattt tttatgattg taacgcatac ggaagaaaag cttatgccta
      241 tccttcaaaa tccttatgga tccatgacag gccaatgggt tgttgtcagt gtaactgggg
      301 tacatattct tgctttcttt atctatatta aatgtattga aagaagaccc tttaaatcca
      361 taggctttaa aggcaatgaa aaaattttta aatatttaaa aggagcatgt attgctgttc
      421 ttatgcaact tatttactgg ggaatcattt taatctttgg ttttggtaaa atagccccag
      481 aagctgttca catgacgaat gcccttggac tttctgccat aggacccgtt ttaattttct
      541 taatcgcttt tcttatacaa ggtgccagcg aagaaattgc tgtaagaggc tggttattac
      601 ctgtactggc aaggcattat aagccagtca ttgccatcat cttttcttcc ctttttttcg
      661 gattcatgca tgcttcaaat ccaaatgtag ccgtactgcc tcttatcaat ctggttttat
      721 acggtatttt tgcagcctta tatgctttat atgatgatgg tttatggggc atttgtgccc
      781 aacattccat ttggaactgg tttatgggta atgttttagg attacctgtc agtggtatgt
      841 ttttaggaca atccagtatt atagaaacaa gtttaacagg acctgaatgg attacaggag
      901 gaggatttgg gccagaagga ggtattattg ttacttttat ttttacagtt gcatcattaa
      961 tactcattaa aaaattaatt cataaaggaa ttatcgttaa aaattaactt ttcaattcat
     1021 atcaaaaaca gataaaaaac aaaagatgct caagttgagc atcttttatc tttttttact
     1081 ttttataacc agccacgttt tttaaccgct tcagcaacac ggttgatgga gatgacatac
     1141 gctgcatcac gcatattaaa ttctttgttt acagctaagt cataaactgc tttaaatgca
     1201 gcagtcatct tagtatccaa tttttgcatc acttcatctt tttcccaata ataattcata
     1261 ttacattgaa cttgttcaaa gtaactgcat gttaccccac ctgcattagc aagaaagtca
     1321 ggaataacaa atactttacg ttcattaagc actttatcag catccggagt agtcggtcca
     1381 ttggctgctt ctgcaataac ttttacctga ctgctgattt tattaacatt ttctgcagta
     1441 atttgatttt ctaaagctgc aggaattaag atatcaacat cttgttcaag ccatgcatca
     1501 ccaggaagaa tttcataacc cagttctctt gctttgtctt tatcaataca gccaaaacgg
     1561 tctgtaatac tcatcaatac atctacatct atgccatcca gctttttgta tgtataggct
     1621 gtcttatcat tgttatccca acaggaaact gctattactt taccgcctaa ttttgtatat
     1681 aactcagctg catattgagc tacattacca aatccctgaa tagcagcagt tgtttttgta
     1741 atgtcaattg ccaattcttt taaagcttct cttaatacaa agataacacc atatcctgtt
     1801 gcttcagttc tgccaagaga accacccata ccaacaggtt ttcctgtaat aaatccggga
     1861 tatttagcac catgaattgc ttcatattca tcaagcatcc ataacatatg ttttgcattg
     1921 gtcattacat caggtgcagg aacgtcctgg ataggaccaa catttcttgc taattgcctt
     1981 acatagcctc ggcaaatttg ttcttgttct ctttcagata aaacacgagg gtcacataca
     2041 acgccacctt ttcctccacc aagaggaata tctactacag cacatttcca ggtcatccac
     2101 atggataaag ctctaatggt atctactgtt tcatcaggat ggaaacggat gcctccttta
     2161 gcaggacccc ttgcatcatt atgctgtata cggtaacctc taaatacttt tgatgtacca
     2221 tcatccattt taaccggaat agaaaaatgg tattctctca ggggctggcg aagtaaatca
     2281 caaattgctt catcaagacc aatttttttt gctacactgt caaactgagc ctgtgcattt
     2341 ttatatgcat tgtagttctc tttactcata ttttacctcc taaacatact ggtttcttta
     2401 tttaactctc aataattata acattccgtt tttttatcgt caatgcagga tgaaaagatt
     2461 taaacttctt ttttgcgaca attcaacaaa ttgtaaattg aaaaatcttg ttctattgat
     2521 agccaataat atcaacaaat ttttgagctg caggagaaag ggatacactc tttaaaaaac
     2581 aaaaaccaat ggctctttta ggaatttctt ctgtcaaatt gattttatat agaagtttac
     2641 tatccaaata gtccttagaa aattcctcaa taacacaagc gattccaaaa ttatattttg
     2701 agaactccag taataattcg tgagaaccca attcaatttc cggcgttagt ttgagtcctt
     2761 ttgctgtaaa aaatttttca acatattgtc ttgaattcga ttttctttcc aaaaaaatca
     2821 aaggatagtt taataattgg gaaagagaaa cttttttacc gacaagttct ttgtattttt
     2881 ccccgcaaac aaaaatatca tgaatatcca tgcatttttt tacagcaatg gctgaatctt
     2941 ttacaggtaa attacaaatg gccagatcaa tttctcctga ctttaagagc gtacataatt
     3001 ctatggttgt tctgtttatg attttaagtt taatattagg ggaattatcg tggaatactg
     3061 ataaataggg gagtaaaaag tacctggaaa tcgtatcacc aacacctatt ttcatttccc
     3121 cggccattaa atttctggct tcaaaaagct ttttctcacc aacattaatt aaattaatgg
     3181 cagaattggt atattcaaac aacaattgtc cctcgtttgt caaggcgacc ccctttgaag
     3241 ttctggtaaa aagtcttaca ccaagttcct tctctaaact cattatagcc tggcttacag
     3301 cgggctgagt catatacaga tttttagctg ctttagaaaa gcttttgcag ttagctactt
     3361 cacaaaaaac tttatataaa tccagtttgg taaccatata agtactcctt atattcaata
     3421 ttaaagctat ttattttact tatatcattt ttttgtgtat aattcaagta tacattattg
     3481 ttaaggagag atgctagatg accagaatca caggaaccat tgccagaggt ctgagaactc
     3541 caattattaa taaaggggat aatcttgtag agattgtagt agataccgta ttaaaagcat
     3601 ctcaagtaga aaatttttct attcgggaca gagatataat cggtattact gaatctgttg
     3661 ttgcacgctc ccaaggcaac tatgcaagta ttgatgacat tgccaaagat attaaagcaa
     3721 aatttggcga tgataccatt ggtatcattt tccctatttt aagccgtaat cgttttgcaa
     3781 tttgtctcag aggtattgca aaaggtgcta aaaaaattgt cttaatgtta agctatcctg
     3841 ccgatgaagt tggaaatcat ttagtagata ttgatttatt agacgaaaaa ggcattaatc
     3901 cctggacaga tgttttaacg gagaaagatt tcaggaatta tttcggctat caaaagcatc
     3961 ctttcacagg tattgattat atagagtatt ataaatcttt agtagaagaa tataaggttg
     4021 aatgtgaaat tattttttct aacaatccca agaccatatt agattataca aaaagcgtcc
     4081 ttgtatgcga tgttcatacc agacaaagaa ccaaaagaat tttaaaagcc aatggagcag
     4141 aaaaagtatt tggtcttgat gatattttga caaaacccat tgacggtagt ggttataatg
     4201 agcactatgg cttgttaggt tccaataagg ccacagaaga aaccattaaa cttttcccta
     4261 accgttgtca ggaaattgtt gaacaaatcc aggctattat aaaagaaaag acaggaaaaa
     4321 ccgttgaagt actgatatat ggagatggtg ccttcaaaga tcctgtggga aaaatttggg
     4381 agcttgccga tcctgttgtt tcaccaggat atacaaaagg tctcgaagga acccccaatg
     4441 aagtaaagct taaatacctg gccgataata attttgctgg cttaaaagga gaagaattag
     4501 aaaaagctat ttctgattat ataaagaata aaaaagaaaa tctcgttgcc agtatggaat
     4561 cccagggaac cactcccaga agactcacag atttaattgg ctctctttgc gacttaactt
     4621 caggaagtgg tgacaaagga acccctgtca tatatattca aggatatttt gacaattatg
     4681 caaaataaac taagcaaaaa gtttataaat aatataaaat aagctgccta ttggcagctt
     4741 attttatata ttaggaattt gccagtcgat ttctgtttga ttgttttttc ttaaaaattc
     4801 attggccttt gaaaaatgcc tgcagccaaa aaagcctctt gaagcagaca atggacttgg
     4861 atgagcagca gtcagtataa gatgtttagg attattaatg tattttattt tttctttagc
     4921 attattcccc cataagataa agaccatcgg cgtttccttt tcattaagca gttgaataat
     4981 ataatcggta aaaaattccc agcccttgtt tctgtgagaa ttggctttat tggcttctac
     5041 agtcaggcag gcatttaaaa gcagcacccc ttgttttgcc cagggaacca gataaccgtt
     5101 attgggtata taacagccca aatcatcctt taactcttta aaaatattga ttagggatgg
     5161 aggaatatct acaccctttt ggacagaaaa ggctaaacca tgggcctgat tttcaccgtg
     5221 gtaaggatct tgtcctaaaa ttaaaacttt tgtatccctg tatgaagtta attttaaagc
     5281 ctcaaaaata ttatacatac tgggataaat cgtttttgtc ctgtattctt ttgcaagaaa
     5341 ctgccttaat cttaaataat agtctttttt aaattcatct tttaataatt catcccaatc
     5401 atttcctata tgaaccacac tgctttctcc tttttattaa tattctttat caaatctctt
     5461 tttaagaaat attaatcttt cttcctcttg ctgtgaaaag ccgccatggc catagtccgg
     5521 atatattctc atttctttat ttgactgaat atgattaaag gcagcaaaac aggtagaagg
     5581 cggacaaatt gcatcttcca ggcccacact cataagaact tctcctttaa tcctggaggc
     5641 cagattcata ttatcaaaat agcttaaagt tttgtaaact tgttcttcag ttttgtgcag
     5701 actatcgtga attttaaaga aatgacttat ttctaaataa ggatactcct gcgctaattc
     5761 aacagctctt ttaaaatggc aaagaaaagg acaatctgcc ataaccactt ttacctcagg
     5821 acaaagagca gctactgcta aagacaaggc acccccctga cttccacctt caacggcaat
     5881 ccgatttgta tcaatatctt ccctttgcat caataaatca atgattcttg cacaatccat
     5941 atatacataa agataatagt aattcttatg attgtaaata cctaaagtca tccagccaga
     6001 agcgcctcca ttgtcataat gattgtgatc agggcttgta acattctggc ctcttacctc
     6061 agcaataaaa acaccgtaac ctaaaagtaa atatttaaag gtatggctaa tcacccggtt
     6121 gttccaatta taaccatgat acataacgac cacaggtact ttattgtctt ttgaagccgc
     6181 agctggcaat attaatcttc catggattct ggaatttcta aagccatcat agtaaacatc
     6241 ataaactttt atctgttcta caggataatc catggctata tattcagtat ttaaaggctg
     6301 tgccttagac tccttaatta tactgtccca aaacaaatca aaatcttctg ctttcgtttg
     6361 attgggtttg tatactttta attcttctac tggtaaatca aaaaaattca tccttatccc
     6421 tccaataatt aagtctccag tatgcatcta ttatactata ctagagactt aaaaatacca
     6481 caagatatat tatttaattt tcttatgttt aaatttctta actttatttg cataatccgc
     6541 tacaatatgc ccattgccaa aaagcttata tttgtaaatt acaagaccat ctaagcccac
     6601 tggtcctctt gcatgaatct tattggtact gataccaact tcagccccaa aaccatatcg
     6661 gaaaccatca ctaaatcttg tagaacaatt taaaaagaca tttcctgaat ctaccaaatt
     6721 catgaataaa tccgcatttt ctttattatt ggtaataatt gcatccgtat gacctgaacc
     6781 gtactgatta atatgatcta tggcttctcc gatatcagaa accagtttaa tggaaagttt
     6841 ataatccaaa tattctgtct tccaatcatc ttcagaagct ggcaatatat cgattatttt
     6901 tctagtcttt tcacaaccat ataattctac ttgctttttt tctaactttt cttttagttt
     6961 tggcaagaat tcttctgcaa ctgcctgatg aaccaaaagg gtttctgcag cattacatac
     7021 cgctacatat tgggtttttg aatcttctac aatatcaact gccatgttta tatctgcatc
     7081 agcatccaca tagcaatgac aaataccatc ggcatgccct aaaaccggaa tatttgaatg
     7141 atccataata taacgcacaa attcatttga acctctcgga atgattaaat taatgtattt
     7201 atccatttta agcatttcat tcacatcatc cctgctttca agcaattgaa tccagccctc
     7261 aggcatattt gctttacgac aggcctcttt tataatttca tataaaatcc tgttggtgtt
     7321 tttagcttca cttccaccct ttaataaaac tgcatttccg ctttttaaac aaagggtaga
     7381 tatttgaacc agggcatccg gtcttgattc aaaaataatg ccaataacgc caatcgggca
     7441 actaacccgg tacagttcaa gaccgtcgtc aagttctgtt attgcttgag tgacattaac
     7501 tggatcatcc aaagcaatta aactcttaat tccttgaatc acatcctcaa tttttccttc
     7561 atcaaatttt aatctcttaa gaagcggcat ggctaaattt tcgtttttgc ttctttctaa
     7621 gtcttcctga ttggctttta tgatttgatt tttatgattt tctaatcctt ttatgatttc
     7681 ttccagggct ttatttttta actcagcgct tgctaccgca agctttattg aagcctgttt
     7741 cgcttctaaa gccatctctt taatacccat agtaccatca cctactctaa tttttacaat
     7801 aataatacta tagctgttat atttatgcaa taatttgtct aggtaaagtt actttaataa
     7861 atattaaaaa tttgcaaaat atagtattga taattagtct tgtaaattat agaatgaaac
     7921 ttatagaaaa gtattgagaa gggagcagca tgttttgagt aaatattgga acagattaac
     7981 aaaatcttta gtaccttacg taccagggga acaacctaag gataaaaaat atattaaatt
     8041 aaataccaac gaaaatccct accccccatc ccctaatgta atagaagcca ttaagtatgc
     8101 tgttaatgag gatttaaaat tatatcctga tcctaatgcc gatgcgttaa gaaaagtaat
     8161 tgcggactac cacggtatta atataaatca agtctttgta ggaaatggct ctgatgaaat
     8221 tttatctttt gcctttgctg ctttttttga ttcttcagag aaaatccttt ttcctgatat
     8281 tacttatagt ttttatccag tatatgctga tttttataat attcagtatc aggaaattcc
     8341 cttaaaagag gatttttctc ttcctataga gcaatttaat atccccaatg ggggcatcgt
     8401 tttagccaat cccaatgcac ccagctctaa atatgtcccc cttgaggatt tagaaattat
     8461 cctgaaaaac aatttggaaa aggtcgttat tgttgacgaa gcatatatcg attttggagg
     8521 tgaatcagcg gttaagctca tcaatcagta tcctaacctg ctggttatcc agaccttatc
     8581 aaaatccaga tccctggctg gactcagggt cggcattgcc atgggaactg atgaattaat
     8641 cgaaggctta aacaggatta aaaattcatt taactcttat actttagaca gactggctat
     8701 agcaggtgct attgctgcct ataaggacaa agaatacttt gaaaaaacaa gaaaaaaaat
     8761 cattgaaacc agggaaaaaa ccatgaaaga attacgaaaa cttggcttta caatgcctga
     8821 ttctatggca aacttcattt ttgtcagtca tccaaaagtg aatgctgaag aaatcttttt
     8881 agctttaaga gaaagaggta ttcttgtacg ctattttaag aagccaagaa ttagtaatta
     8941 tttaagaatc agcattggaa ctgatgaaga aatggaaata ttgattaata atttaaaaga
     9001 aatcatcaaa taaaaattgc ttttccaagg aaaagcaatt tttattttgc aaatatatca
     9061 ataacttttc atgcttcatc accagcaata aataataccc caagggtatt aggcccgcaa
     9121 tgagaggaga taacgcctcc tgccctggta atcaaaattt cttcaaagta atttaagcct
     9181 tttagataat catagacttc ttttattatt tcttcactac aaccagagtg tgtgataaat
     9241 attcttctct tatcagcttt taataaatcc tcttctaagt cttttgcata ttttaaaatg
     9301 actgatttta tattacccct gtatttttta ccaacatcca ttttaccgtc ttcaacaata
     9361 attttaggtt ttaattttaa ggtgtttgcc aacagggaag tcactgctga acatctgcca
     9421 cctctatata agtatgtcag ggtatctatc acaaaacttg cccgaacctt aggaataatt
     9481 ttgtccattt cttttagaat gtcatctgcc gaagcacctt ccatagccat ttctgctgcc
     9541 ctgagaacct gaaggcctat acctgtagaa agattttttg aatcaatgac ccatattttt
     9601 tcatagtcca aattttccgc tgctaaacgg ataacaccgc aagtagccga catagcttca
     9661 gatatcccaa aaaatataat atcgtctcct ttttgtgtaa aaggtgttaa aaaatcaata
     9721 gcatccccaa gggaaggaca tgcggttttc ggcgttttcc caaccttatc tgaccattgg
     9781 tatatttcgt ccggggtaat ttctacaccg tctttgtaac tgtctccttc taaattaatc
     9841 atcagtggaa gaatttttat attatacttt tccaataact cctgcgataa atcacaagta
     9901 ctgtcagcaa tgatttgtac catatatatc cccctattct ttaaattaaa ggttttccgc
     9961 aatatggaca ataagcatat ctgtcatcat caacaaaagc accgcaatat aagcatttgc
    10021 ctttttctgt gatcgtattg aatccagata aatcttcatt tcttaaatct acaattttgc
    10081 cttcttcaat ttcttttccc aactgggaat caatcataaa agctttcctg caacaagatg
    10141 ttcgtatata atattttata ttccacttaa atatcggaat gaaaaatata tgaaaatagt
    10201 cataagtttt tataatttga taacttccta aagcttcgca agacggacaa atggtatttt
    10261 gtattgctgt aatttctttt tcttttgtct gtatgccaaa tatcccaata aaaaacattt
    10321 acaaaactcc ttatgatgta ttttcacatt aagccattat tatatattat attatcattt
    10381 atacgaatcc ataacaagct tgttttagaa agggaactat catgcaatta aattataata
    10441 ctgtatatgg tacttttatt caaagaccta accgttttat cgcttatatt gatgtagatc
    10501 atcaaaaaat tagctgtcat gtccctaata caggaaggct gaaagaactg ctgtatccag
    10561 gagctcaagt tatcctttcc catcatcctt caggaaagcg gaaaacagac tacagccttc
    10621 gaatgattaa aaagaaaaat cactggatat ctatagattc ccaattaccc aatgcccttg
    10681 cctatgaagc cattttaaat ggaaacattg aagaattgag aggatattct atggttaaaa
    10741 gggaaatctg ctttggcaaa agccgctttg atttgcagtt aagtggaaac gacacttgct
    10801 ttgttgaagt caaaggcgtc actttagaaa aagacaactg gagttatttc cccgatgcac
    10861 ctactcagcg gggtcgtaag catattgatg aaatgattgg tgtcattgaa aaaggatatc
    10921 ggggagtcat tctttttatt gtacaaatac catatgccaa aggctttagt cccaattggg
    10981 ctaccgatcc gggctttgct caaaaattaa aagaagcctc agaaaaaggc gtagaaatcc
    11041 tggcctataa gtgccatatc agtcctaatg aagtttttgt tactgaaaaa atacctgtta
    11101 ttctgtaaat aaaatttttt taagtataca tgtcttaatc ataaaaatcc ctctattgag
    11161 ggattttatt cttctttctt aaaaattata tttttttaat aattaaagac aactaacttt
    11221 ctaaaccagt ttcttcagga agtcccaaaa caatattcat attttgtact gctgcaccag
    11281 aagccccttt tcctaaatta tctaatctgg caaaaattaa actcatttct ttttcgtcat
    11341 tgccaaagac aaatatttca cattggtttg tgtcattgca tgctgtaata tcaaaagcac
    11401 catcaaataa taaatccatt ggattgtaag gcatgacttt aacaaaacat tcatccttat
    11461 aatattcaga taaaagttca gcaatatctt tagctccagc ttttttatta agtaaatgat
    11521 tatgtatggg aaccgatact gctaaaccct tataataatt acttacaaca ggaagaaaaa
    11581 ccgggtatct gtccaaaccc gtataaactt tcatctctgg cagatgctta tggtttaggt
    11641 ttaaaccata aggccttggt gctttaatat aaggtgcatt tatttcctca ttttcatatt
    11701 tttcgattaa agacttgccg ccaccactgt aacccgtgat agaatgagcc gttacaggat
    11761 aatcggatgg cataagtccc attttaacta agggtgcaac agcaagaata aaagcactcg
    11821 cgtgacagcc aggaacggtt acccttttgg attcacgaat tttttctctt tgttcctttg
    11881 ataattcagg cattccgtaa gtccagccat ctgctaccct atgagcaaca cttgggtcta
    11941 taattttagt agtaggattg cttacaagct tagccgattc cctggcagca tcatccggca
    12001 aacataaaaa tacgatatcg gcctcattca aaaacttctt ttttatttct gtatcttttc
    12061 ttttgtcata gtctatggtt aacaattcta caaagggatg cttttttaat ctttcatgaa
    12121 tttgaaggcc tgttgtacct gtttgaccat caacaaatac cttatatttt ttcataagcc
    12181 tgtcctccaa aacttattta tgaaaaaata ttcaaaaact aatatttctt atcctatttc
    12241 atcaactcta ttaaataatc atacatcatt atgaatattt attcaagtac tctttataaa
    12301 aaacgaatat ctatttcaca aatatttttg accactatat ctgcttttga taaatcctgc
    12361 ctgcctgaat tagggttaat atatccaata catttcatgc ccgcagcttt tgctgccatt
    12421 acaccatttc gggaatcctc aagaaccaca caagcttcag gattcacctt caataatttg
    12481 gctgtttcca ggtaaatatc aggggctggc tttccttttt ttacttcttc tccgcttaaa
    12541 acaatatcaa aataatcata taaagcaagt ttatttaata tctctttaat taataattta
    12601 ggcgaagatg atgcaaccgc aataggtatc ttcttttcac aaagattttt aattaatttt
    12661 tctatacctt caataggaga aatatctgtc tcccttactt tcctgatttt ttctttaatt
    12721 tgttcttcta aaattttttc cgtcgtttca tgaataggat attctttttt tattaattcc
    12781 cacatctcat aatcggtcat tcccgcatac ttttctaaaa agtctgcccc cttctgaata
    12841 ccaaaagacc ttaaacctgc taactccact tcaaaatgaa tcggctggct gtcaataatg
    12901 actccgtcca tatcaaaaat aaacgcttcc attttagtcc tccttattca ttcgctataa
    12961 atttattggt gtctttatca taaatataac caatcgaatt gattttttct aagacttctt
    13021 cttcctttaa attatatctt tcacataatt cttcaaggtt tttacattca tttcttaatt
    13081 tcatgtttaa ccagcttaaa aggatataat tgtctcctaa aggtacatat tgactcatta
    13141 ttttcctcct ttttgaagct tttattacaa catttcaaga aagtcatctt atgcattagt
    13201 gatatcatat atacaatctg ttaaaattac aagtgttttg gtcttatttt tctttaaaaa
    13261 aataacttag taataatccg gggattattc taagttatat tcatataaat attttatatc
    13321 ttgtcttcca cgatgcatat cattgagcaa atctaaaaca ctgtctatag ctaatggtct
    13381 ggcaaacaaa tagccctgga aataatcgca attatgcttc attaaatatt ccacttgctt
    13441 cttctcttct acaccttccg ctactacctc catgcccagc ttatgcgcca tggatataat
    13501 taattcaacc aaagatttat tgggatccgt aattagaatt ttatctacaa aagctttatc
    13561 gattttcagg atattcatgt gattgtattg taaaaaactc aaagcagaat aacctgtgcc
    13621 aaaatcatct aaagcaattg ttatacccag gtttttaaaa taattaataa ctctttcagc
    13681 acatttttct gaggaaataa aaacagattc tgtgatttca aattcaaggt tttttggatt
    13741 aaaatcattt tcttttaaag ccctttcaac cttagatata aacatattat ctgttaattg
    13801 aacggatgat atattaatgg acaatttaaa attatcattg tactttaata tgttattttt
    13861 aaattgacgc atggcttcat ttaataccca atgaccaatt tcaataatac tattattttc
    13921 ttcagcaatt ggaataaaaa ccgaaggact aataggaccc aattctttag aattccaacg
    13981 gagcaagacc tcaaatcccc ggatttcatt tttataacta taatattgag gttggtaaac
    14041 taaataaagc tccttattcg aaatcgcttt ttttaattca ttttccaatt tcattcgggt
    14101 aagcaacttt tctttaatat cattagaaaa aaaggatata ctgttcttac catttttttt
    14161 agcatgctgc atggaaatat ctgcatgttt taaaatatca gaagccgttg gaccgtcttc
    14221 tggatataaa gcaacgccaa aactggccgt taataaaaat tccttatctt ctatgaaaaa
    14281 aacatctgta agctctcttc tgatttcatt taaatattct aaaatttctt ctttggataa
    14341 atcgcgttgg ataataattg caaattcatc accatcagtc cgtccaaata agtcctcttt
    14401 atggattttc tctttaattc tctctgcaat ttttttaagt aataaatctc ctacaagatg
    14461 tcctgctgta tcattaacgg cagtaaaatt atctatgtct aaaaatacta atgcaaattt
    14521 tcctttattc tttgataaat ttgaagttaa tttgcgaacc ttatgtataa tcacattacg
    14581 attggtaatg cctgttacag aatcaaaata agccaactga tataattgtt ttttgcgtct
    14641 ttctccttca accaaacaat tattaattac cgtcaaaatt aaaaatatac ctaaagctgt
    14701 aacgattcca gcaataacgc catccgaatt tcttttaaat aaagagaaaa gcatcaaaat
    14761 gaaattaatt gcatttaata ataaagcgat tgaataacct tttttgttat taaagtaaag
    14821 tactaaaaaa accagaatta ataattgtaa ttgcgtaata ataccattta taacaatagg
    14881 attaagatta tatgcagtta attggctgaa catagggtgt gagcttagaa atacaagtaa
    14941 aatcaaagta aaaaaataaa ctgttactaa taaaatcata acgatttttt cttctatttt
    15001 ttttaacagc ttatccatct tatcccccat tattcaattt tattcaccct caaaattaaa
    15061 ccacccacag ataaat
//
LOCUS       DAOSUD010000052        14930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2426_length_14930_cov_24.989042, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000052 DAOSUD010000000
VERSION     DAOSUD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_14930_cov_24.989042"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      51..237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            416..1408
                     /locus_tag="PLG49_07500"
     CDS             416..1408
                     /locus_tag="PLG49_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ17202.1"
                     /translation="MKRFKTLLSNIFIMAIISAFIVSCSAKAGQRAEENMNIRMQEEN
                     KRESASSKEAFTIVDRAGNEIIIPEEINSIISLAPSITEILIDLGFGDKLVLVDAQTK
                     ALVDLPKDIESVDLMTPDTEQMIVLKPDIVFASNITSIGGTDPTAQLTAADITIAFIP
                     TSNSIEEIYEDIMFIAQVLQVEDKGEALVNQTKERIAKIEEISKTIEEKDKKTVFFEI
                     AALPQIYSFGSNVYLNEMIELIGAKNILASKEGWMSVSEEAIVAADPDVILTNVNYIE
                     EPVEEIKSRTGWENVKAIKNNNVYYIDNKTSSLPNHNIVQALEEMAKAVYPDKY"
     gene            1422..2444
                     /locus_tag="PLG49_07505"
     CDS             1422..2444
                     /locus_tag="PLG49_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ17203.1"
                     /translation="MKTKIKIFISILSSIIVLVLGVAIGSVYVEPLKILTIIFGKILS
                     QPISADLNENLISIIWDIRLPRVLAAFLVGGALAASGTVMQSILKNPLASSYTLGVSS
                     GASVGVGLVLITGFSIPVLGNFTLPLIGLLSGLVTVYLVITITAQIDRNMGNTTIILF
                     GMNFSLFINAVTTLIMALSRETLPRLIYWQMGSFALKSWLDVKILAVIFVGCYLILIR
                     YTNELDIMTFGEEQALAIGVEAKKVKWILLILSAALTGSAVAFTGVIGFIDLIAPHVV
                     RKIFGSSHKLVVPISILFGGTFMVACDLVARILVSNSELPVGAVTALIGAPFFAYVYF
                     KNRKVR"
     gene            2446..3213
                     /locus_tag="PLG49_07510"
     CDS             2446..3213
                     /locus_tag="PLG49_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17204.1"
                     /translation="MIEVKNVSAGYSGIDVIENISLKLEVGLNLSIVGPNGCGKSTLL
                     KAIAGIIPIKGDVLIDGKSIKTMKRAEIAKKIAVMSQMSAIYFSYTVFETVLLGRYVA
                     MKDNFLKEASQQDKAYVEKCLEAVGMLEYKDRQIDCLSGGELQRVFLARTLAQEPEII
                     LLDEPTNHLDLKCQHELILYLKNWSKDHKHSVIGVFHDINHAMELADHLLVLEKGHIK
                     ALGKAEEIIKSGLLNDVYEMDIKKYMLNSLKKWEFVN"
     gene            3223..3753
                     /locus_tag="PLG49_07515"
     CDS             3223..3753
                     /locus_tag="PLG49_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HOQ17205.1"
                     /translation="MKEKRYKSKFEAYPFLSEPANDLKCDFEILTDEIASHIGLLHSL
                     VKDDAIKNELLIICELTYHINPSLRTFISVTEDELEYLETATKRLREETKDRCDKFVL
                     PVGSEGAGHAHIIRSKCKSLIRLLYRYDEQGNEVPNLLFDFTNLLSAYFFYLALKLNQ
                     LEGISEIEFKSRNYHQ"
     gene            3798..4871
                     /gene="cobT"
                     /locus_tag="PLG49_07520"
     CDS             3798..4871
                     /gene="cobT"
                     /locus_tag="PLG49_07520"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOQ17206.1"
                     /translation="MNLLKETISKITKPDEKAREEARKKWDGLLKPIGSLGVLEEITM
                     KISAMTGKVINHFDKKAIVVMCSDNGAVEEGISAAPQIFTKILAESMPKGLTGVATLG
                     KFTNTDIITVNLGIKGHIDNPEVIDKKIADGTKNFTKGPAMTYEEAIKAIETGIEVAD
                     QLYAKGYDILGTGEVGIGNTSTAAAVVSGLLGLEVDTICGKGAGLTEEQFIHKKEMIK
                     KAISINKPNKDDPLDVISKVGGFDIAGMSGLCLSAAKNKKPIVIDGFISSAAALCASY
                     LNPYVKEYIIPSHLSAEPGARYVLDVLGLKPLLTLEMRLGEGSGCPLAFQILEAAEYV
                     LENMGTFDDVSMSSSSLIDIREK"
     gene            4881..5420
                     /gene="cobU"
                     /locus_tag="PLG49_07525"
     CDS             4881..5420
                     /gene="cobU"
                     /locus_tag="PLG49_07525"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOQ17207.1"
                     /translation="MVITLVTGGARSGKSKFAESLFKSDEDVVYIATARITDKEMEER
                     IKHHQSFRPKHWRTCEANYNLKDAIGNEKNYLLDCITVLTSNIMYDISKDKESIDLEI
                     QQTIEDSVFCELKELMDEIRKKGYHLVIVTNEVGYSIVPSHPVSRAFRDIQGRINQRI
                     AAEADQVYLVCCGIPVKIK"
     gene            5417..6154
                     /gene="cobS"
                     /locus_tag="PLG49_07530"
     CDS             5417..6154
                     /gene="cobS"
                     /locus_tag="PLG49_07530"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOQ17208.1"
                     /translation="MIKGLILSIQFFTRIPIKIQVDFNEKNIRYCIFFMPLVGAIIGS
                     LSSIIFYLLEPHNKLIAAFLTLLTSIILTGGLHIDGLADTFDGFLANKDRQKTLEIMQ
                     DSRIGSFGVLAIVLHIIFILILILSIENLPLVLILSFVNSRLVASQIIAFKKPARSDG
                     LGNLFHKSNPSKVTIINTVIYLIIIILIDIKYLIPLGINYLFAEFISHIACKKIDGLT
                     GDVYGAIIELGVAVSLFSFWSVMTWIS"
     gene            6142..6708
                     /locus_tag="PLG49_07535"
     CDS             6142..6708
                     /locus_tag="PLG49_07535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ17209.1"
                     /translation="MDIIMIRHGQSEDNLRKVYSSQSTPLTKEGIAQILKTKTLLKGY
                     TYEEVYYSPYKRTVESLHYLDLKGKEETRLREIDFGIFTGKSYEEILKIYPQETNLWI
                     NDSYNYRIPKGESLSEVYKRVTGFLEEVSQSNKNTLLITHDTVIRLSLCWVFDNPLFF
                     YKFKIDEGSITTIRISEDYKYIYRVNQT"
     gene            complement(6777..7226)
                     /locus_tag="PLG49_07540"
     CDS             complement(6777..7226)
                     /locus_tag="PLG49_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17210.1"
                     /translation="MKVEKISDTDKSKYMDLLLIADEQVDMIEKYLYRGEMFALYDDD
                     LKALCVVTQEGPGIYEIKNIVTVPKYQRQGYGQHLVSYIIDYYKDSGTELYVGTGDSP
                     MTLNFYEKCGFKRSHVVKNFFTDNYDHPIYENRKQLVDMIYLKRPLG"
     gene            7412..7954
                     /locus_tag="PLG49_07545"
     CDS             7412..7954
                     /locus_tag="PLG49_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ17211.1"
                     /translation="MLKHGILGLLNYGDMTGYEIMKVFRDSLNFFWAAKTSQIYRELQ
                     TLKKEGFVTDVLVKQTGKSDKKVFSITESGKKELKRWLREYNYGNTNNALCMKVFFSG
                     ELSKEENIERFENIKKEAQEAITRYSAIFELTKAYSKMLVNPEKAAYWDMTVDYGIRY
                     MKMLVEWCDACIRELGDVKE"
     gene            7951..9555
                     /locus_tag="PLG49_07550"
     CDS             7951..9555
                     /locus_tag="PLG49_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ17212.1"
                     /translation="MKILIINGSPKGDSSNTLKLTKAFVEGMGDHEIKEITVSKLNIS
                     ACKGCFSCWGKSPGKCIIDDDMTQVIEDELWADIILWSFPLYYFSVPGSLKNLIDRQL
                     PMVLPFMTEREDGIGAGAHPARYDMSGKRHVLISTCGFYSAEKNYDSVKSMFDYILGK
                     GNYETIFCGQGELFRIPELKGRTDEYLHIVRKAGKEYISSGISNTTREKIKELLYPKE
                     AFEEMADASWGIEKEDGGKVDQSLSFTKQMAALYNKESYDGKDRVLEICYTDLGKTYQ
                     MLLGKDGCQVFTDGRLTATTRIETPWEVWLSIARGEIRGDVALAKGMYKVKGDFSLMT
                     NWDKVFPRSSQVNNGLSDGDSEAKKPAMLTMLIPWITFWLAVSINASSGAVITLAVCG
                     CLPLIMARKALTIYDKFSLSLISLLSVLALQDNMQIIAIVAAYLAFGLMWLLSCLTKE
                     PICAAYVKYDYNGDDALNNPLFMKTNYILAASWGILYLLIAVWSWFLLNFNQNILLQV
                     LNNAAVIAMGIFTTWFQRWYPVYLAK"
     gene            complement(9608..9793)
                     /locus_tag="PLG49_07555"
     CDS             complement(9608..9793)
                     /locus_tag="PLG49_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOQ17213.1"
                     /translation="MIKFLINNLSTIIISVILLLIVASIIYKQIANRKKGKTSCGCGC
                     SACSAYSLCHPESEKSK"
     gene            complement(9818..12220)
                     /gene="feoB"
                     /locus_tag="PLG49_07560"
     CDS             complement(9818..12220)
                     /gene="feoB"
                     /locus_tag="PLG49_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOQ17214.1"
                     /translation="MSIKIALAGNPNSGKTTLFNALTGSNQFVGNWPGVTVEKKEGRL
                     KKHNDVIVVDLPGIYSLSPYTLEEVIARNFLITEKPDVILNIVDGTNLERNLYLTTQL
                     TEMGIPVVVAVNLMDAVEKNGDKIDLLALSEKLGCKVIGISALKGTGVDEAANAAINA
                     AKSSTQPAHKFTEKVEDTLAQIQKTVSLPTGQERFFAIKLFERDEKAMESLEIAPNVL
                     SEIEELINACEKDLDDDSESIITNERYEYIASIIDSCYIKKNKGKLTTSDKIDRIVTN
                     RWLALPIFAVIMFLVYFISVTTIGTFATDWANDGVFGDGWFLLGIGSSAYQEAVEEYE
                     ESSTILETFETAAKEAGIEPVEAVELVAPAEFYDEEGNLEETIMVNYEIYTQIGEIKE
                     PDPSDYGIWIPGVPVLVGNFLEKINTAEWLQGLILDGIVAGVGSVLGFLPQMLMLFFL
                     LAFLESCGYMARIAFILDRIFKKFGLSGKSFIPMLVGSGCGVPGIMASRTIENDRDRK
                     MTIITTTFIPCGAKIPIIALISGAMFGGAWWVSPSAYFVGIAAIICSGIILKKTKLFA
                     GDPAPFVMELPAYRMPTLSNLLRSTWERGSSFIKKASTVIMLATILVWLLSTFGFDDN
                     GFGMVEMNNSLLATFGNLLAPIFAPLGWGHWQGTVATLTGLIAKENVVGTLGVLFGGL
                     DEVAENGWQIWANMQESFTPLAAYSYLVFNLLCAPCFAAIGAIRREMNDAKWTLFAVA
                     YQTIFAYSISLMIFNIGNLIITGSFGPGTIVALALLAIFIYLLFRPQASSRAKQKAIT
                     SA"
     gene            complement(12316..12549)
                     /locus_tag="PLG49_07565"
     CDS             complement(12316..12549)
                     /locus_tag="PLG49_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOQ17215.1"
                     /translation="MLTLKDIAIGQNIVVKKFHGEGAVKRRLMDMGITKGARLHVKKV
                     APLGDPIEVTIRGYELSIRKEDAEKIEVELINE"
     gene            complement(12576..12785)
                     /locus_tag="PLG49_07570"
     CDS             complement(12576..12785)
                     /locus_tag="PLG49_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ17216.1"
                     /translation="MPLTFAKIGERKTIKKISGKDSIKRFLESLGFVEGTEVTVLSDI
                     HGNLIVNVKESRIALSKEMACKIVV"
     gene            13360..14298
                     /locus_tag="PLG49_07575"
     CDS             13360..14298
                     /locus_tag="PLG49_07575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17217.1"
                     /translation="MEILNYKQKYEKEVVDLWNKTLTADPITIHKFRKQILFDENFDP
                     ELCYLAAEDDRVVGFLLSTKRKFPYLERGLEPTRGWINVMFVDQDYRRKGIGTALVEH
                     AEKKLKSLGVETVTLAAYSPNYFFPGIDKDNYSDAVKFFEKMGYQPGKESYSMCKDLH
                     GYQLSQDTLEKLKKAQEAGFSFINFEYKYALELLEFLKDEFGGGWKRNALISMQNNLA
                     EDCILLVLDSQEKIVGFCMRMIDGNPMRFGPIGVKKEARNYGIGGILFDLMQFEMAKR
                     GIFHLFFLSTDEPGRRFYERHGVKVYRTFVDYQKTI"
     gene            14327..>14930
                     /locus_tag="PLG49_07580"
     CDS             14327..>14930
                     /locus_tag="PLG49_07580"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOQ17218.1"
                     /translation="MSEIKRVVSVGAHSLDAEILGGPVMLKYAKQGAHCTYVHVTQGR
                     LEDPNATEEEKQAYLKELLNQNKRAAEKLNGDCLWLGYVSSNMPSLEEFASRMEQYFI
                     DEKVDLVITHWRGSMHPRHINTSDAVTTAVKCLREKGNPIRLVYGENFEDLVGFIPQA
                     YFKLEPEEVEQWFSALREYSVFCGKVNDFPYNDYYTTIGKV"
BASE COUNT         4939 a         2388 c         2972 g         4631 t
ORIGIN      
        1 ttgacatcat gaagttaata tattagactt agtaattaat cagataaaaa ttaaatattt
       61 cgggtattgg tactgaaaag tttaataggg aattaggtga gaatcctaaa cagtcctgct
      121 gccgtattga gcgagctatt ttttgtgcga aagcagccac tggagtaaat ctctgggaag
      181 gtaaaaaata gtgatgacct ctaagtcgga atacctgcca atatcttgcg tattaccgca
      241 aatattatac cttgcagagt acagggtgat agtataaata taggatttta gataaatttc
      301 gcaagaaatt atctaaaatt ctgattattg tggttaatac gtttcggatt ttccgaaact
      361 tttttatttg ttcaaaatca tatgcattta ataataagca gaaaggggag ctaaaatgaa
      421 aagatttaaa actcttttgt caaacatttt tattatggct ataatatctg catttatcgt
      481 gagttgttct gctaaggctg ggcaaagggc agaagaaaat atgaatatac gaatgcagga
      541 agaaaacaag agagaatcag ccagttctaa agaagctttt acgatagtcg atcgggcagg
      601 caatgaaatc atcattcctg aagaaataaa cagtatcatt tctttggcac catctattac
      661 tgaaatttta attgacttag gatttggaga taaactcgtt ttagtggacg cgcaaacaaa
      721 agctttagta gatttgccaa aggatatcga atcggtagat ttaatgacac cagatacaga
      781 acagatgatt gtattaaaac cagatatcgt ttttgcttct aacattactt caattggagg
      841 aacagatcct actgctcaat tgacagcagc agatataacg attgctttta taccgaccag
      901 taacagtata gaagaaattt atgaagatat tatgtttatt gctcaggtct tacaagttga
      961 agacaagggg gaagcattag tcaatcagac aaaagaaaga atagctaaaa tagaggaaat
     1021 ctctaaaacg atagaagaga aagataaaaa aacggtattt tttgaaatcg ctgcactgcc
     1081 gcaaatttat agttttggaa gcaatgtata tttaaatgaa atgattgaac ttattggagc
     1141 aaaaaatatt ttagcaagta aagaaggctg gatgtctgta tccgaagagg ccatcgttgc
     1201 tgctgacccg gatgtaatcc ttacaaatgt caattatatt gaagaaccag tcgaagagat
     1261 taaatcacga actggttggg aaaatgtgaa agcaataaaa aataataatg tttattatat
     1321 agacaacaaa acaagttccc ttccaaatca caatattgta caagcattag aggaaatggc
     1381 aaaggccgtt taccctgata aatattaagg atatgaagac tatgaaaaca aaaataaaaa
     1441 tattcatttc catccttagt agtatcattg tattggtttt aggtgtcgct attggtagcg
     1501 tttatgtaga gcctttaaaa atcctgacca ttatttttgg aaaaattttg agtcaaccaa
     1561 ttagcgcaga tttaaatgaa aatttaatat cgattatttg ggatataaga ttgccaagag
     1621 ttttagcagc ttttttagta ggtggtgctc ttgctgcaag tggtacggtt atgcagtcaa
     1681 tccttaaaaa tcctttagcc tcttcgtata cccttggggt ttcatcgggt gcttcggtgg
     1741 gagtaggtct ggtgcttatt acaggtttta gtatacctgt tctgggcaat tttacccttc
     1801 ccttgattgg cttattatca ggccttgtta ccgtttatct ggttattacc attacggctc
     1861 agattgatag gaatatggga aatacgacaa ttatattatt cgggatgaat ttttccttat
     1921 ttataaatgc tgtaaccaca ttaattatgg ccttatccag agaaacctta ccgagactta
     1981 tttactggca aatggggagt tttgctttaa agtcatggct tgatgtgaaa atcttagctg
     2041 tcatttttgt tggttgttat ttaatcttaa tacggtatac caacgaatta gatattatga
     2101 cttttggaga agaacaggcc cttgctatag gggttgaagc aaaaaaggta aaatggattt
     2161 tacttatatt atcagcagct ctgaccggta gtgctgtcgc ttttactggt gtaataggct
     2221 ttattgattt aattgcgcct catgtcgtaa gaaaaatatt tggttcgtcc cataaactgg
     2281 ttgttccaat ttctatactt tttgggggaa cctttatggt ggcttgtgat ttagtcgctc
     2341 gcattcttgt ttccaattca gaacttcctg ttggagcagt tacggcttta attggagcac
     2401 ccttttttgc ctacgtatat tttaaaaaca gaaaggtcag ataagatgat agaagttaaa
     2461 aatgtatcgg cgggatattc tggtattgat gtcatagaga atatttcttt gaagctggaa
     2521 gtagggctta atttatccat tgtcgggcct aatggatgtg gaaaaagtac gcttctgaaa
     2581 gccattgctg gtattatacc cataaagggt gatgtcttaa tcgacggaaa aagcataaag
     2641 acaatgaaaa gggctgaaat tgcaaaaaaa atagctgtta tgagtcagat gtctgcgatt
     2701 tatttttcat atacggtctt tgaaaccgtt ttgttaggtc gttatgtcgc aatgaaagac
     2761 aattttttaa aggaagcttc acaacaggat aaagcttatg ttgaaaaatg cctggaagct
     2821 gttgggatgc tggaatataa agacaggcaa attgactgct tatctggtgg agagttgcag
     2881 cgggtctttt tagcaagaac cctggctcag gaacctgaaa taattttact ggacgaacca
     2941 acgaatcatt tggacttaaa atgtcagcat gagcttattc tttatcttaa gaactggtca
     3001 aaagaccaca aacattcagt cattggggtt tttcacgata taaatcatgc aatggaatta
     3061 gcggatcatt tattagtatt ggaaaaaggt catatcaaag ctttaggtaa agcggaagaa
     3121 attattaaat cgggcttatt aaatgatgtc tatgaaatgg acattaaaaa gtatatgtta
     3181 aattctttaa agaagtggga atttgtaaat tgaggggata ccatgaaaga aaaaagatat
     3241 aaaagcaagt ttgaggctta tccattttta agcgagcctg caaatgattt aaagtgcgat
     3301 tttgaaatcc ttacggatga aatagccagt catataggtt tacttcattc tttggtgaaa
     3361 gatgatgcaa taaaaaacga actgcttatc atctgtgagc tgacatatca tataaatcct
     3421 tctctaagaa cctttatctc tgttacagaa gatgaattag aatacttaga aacagcaaca
     3481 aagcggttaa gggaggaaac aaaagacaga tgcgacaaat ttgttttgcc tgttggatct
     3541 gaaggtgcgg gacatgccca tattatccgc tcaaaatgca aaagtctgat aaggctgcta
     3601 taccgctatg atgaacaagg aaatgaagtc cccaatcttt tatttgattt tacgaactta
     3661 ttatctgcct atttctttta cttagcttta aaacttaatc aattagaagg gattagtgaa
     3721 attgagttta aaagcagaaa ttatcatcag taaaagaaaa cgttaccgat aaaaaatatg
     3781 tataggtggt gtagaatttg aacttattga aagaaaccat aagtaaaatt acgaagcctg
     3841 atgaaaaagc cagagaagaa gccagaaaaa agtgggacgg gctgcttaaa cccataggca
     3901 gtttaggcgt attagaagag attactatga aaatatccgc tatgacgggc aaggtgatta
     3961 atcattttga taaaaaagcg attgttgtca tgtgtagtga taatggtgca gtggaagagg
     4021 gcattagtgc ggcaccacaa atctttacaa aaatactggc cgaaagtatg ccaaaaggac
     4081 ttacgggcgt tgcgacttta ggcaaattta caaatacaga tattattacg gttaatttag
     4141 ggattaaagg tcatatcgat aatcctgaag tcatagacaa aaaaattgca gatgggacaa
     4201 agaattttac aaaaggtcct gccatgacct atgaagaggc tattaaagcg atagaaacag
     4261 gtattgaggt tgctgaccag ttatatgcaa aaggctatga tatattagga actggtgaag
     4321 taggaattgg aaatacttca acagcagctg ccgtcgtatc tggtctgtta ggattagagg
     4381 tggataccat ttgtggcaaa ggagctggtc ttacagagga gcaatttatc cataaaaaag
     4441 aaatgataaa gaaggctata tctattaata aacccaataa agatgatccg cttgatgtga
     4501 tatcaaaagt tgggggtttt gatattgccg ggatgagtgg actgtgttta tctgcagcta
     4561 aaaataaaaa gcccattgtt attgatggtt ttatttcttc ggcagcagcc ctgtgtgcaa
     4621 gttatctaaa tccctatgta aaggaatata ttattccatc ccatttatcg gctgaacctg
     4681 gtgccaggta tgtattggat gtactgggtt taaagcctct tttaacgtta gaaatgcgct
     4741 taggggaagg ttcaggttgt cctctggcat ttcaaattct tgaggcagca gagtatgtct
     4801 tagaaaatat gggaactttt gatgatgttt cgatgagttc ttcatcttta atagatataa
     4861 gagaaaaata aggagtgttt atggtgataa cattggtgac cggtggagcc agatcgggca
     4921 aaagtaaatt tgctgagagt ttatttaaaa gtgacgaaga tgttgtttat attgcaacgg
     4981 ccagaataac agataaggaa atggaagaaa gaattaaaca tcatcaaagc ttcagaccaa
     5041 aacattggcg tacctgtgaa gcaaattata acttaaagga cgccataggg aatgagaaaa
     5101 attatttact ggactgtatt accgtgctga catcaaatat tatgtatgat atatctaaag
     5161 ataaagaaag cattgactta gaaattcaac agacaattga agattcggta ttttgtgagc
     5221 taaaagaatt aatggatgaa attcgaaaaa aaggctatca tctggttatt gtgaccaatg
     5281 aagtgggcta ttctattgta cccagccatc ctgtatccag ggcattcaga gatattcagg
     5341 gacgtatcaa tcagcgcatt gctgctgaag cggatcaggt ctatttagtc tgttgtggga
     5401 ttccggtgaa gataaaatga taaaaggttt aatattatcc atacagtttt ttacgagaat
     5461 accaataaaa attcaggttg attttaatga gaaaaatatc cgctattgta tattttttat
     5521 gcctctggtc ggagccatta ttggttcttt aagcagtata atattttact tgttagaacc
     5581 tcataataaa ctcattgctg cctttttgac acttttaacc agcattattt tgacgggcgg
     5641 gcttcacata gatggcctgg cggacacttt tgatggtttt ttagccaaca aagaccgcca
     5701 aaaaacttta gaaataatgc aagacagcag aataggaagc tttggggtac ttgctattgt
     5761 tttacatata attttcatat tgattttaat attaagcatt gaaaatttac ctttggtcct
     5821 aatcctttca tttgtaaaca gcagattagt ggcaagtcag atcatagctt ttaagaagcc
     5881 ggcaaggtct gatggtctgg ggaatttatt tcataaaagc aatccaagta aagtaactat
     5941 aatcaatacg gttatttatt taattattat cattttaata gacataaaat atctaatccc
     6001 ccttggaata aactatcttt ttgcagaatt catcagtcat atagcttgca aaaaaataga
     6061 tggtttaact ggagatgtct atggggccat tatagaatta ggcgtggctg tttctttgtt
     6121 ttctttttgg agtgtgatga catggatatc ataatgatta gacacgggca aagtgaagat
     6181 aatttaagga aagtttacag cagccaatct actcctttaa cgaaagaagg gattgctcaa
     6241 atattaaaaa caaaaacctt attaaaaggc tatacttatg aagaagttta ttacagtcct
     6301 tacaagagaa cagtagaatc attacattat ttagatttaa aagggaaaga agagaccaga
     6361 ttaagggaaa tagattttgg aatcttcaca ggaaaaagtt atgaagaaat attaaagatt
     6421 tatccgcagg aaaccaatct ctggataaat gatagctata attaccgaat cccaaaaggt
     6481 gaatcattat cagaggtcta taaaagagtg actggttttt tagaagaagt aagccaatcc
     6541 aataaaaaca cactcttaat cacccatgac actgtaatcc gtttatcttt atgttgggta
     6601 tttgataatc ctttattttt ttataaattt aaaatagatg aaggcagtat cacgacgatt
     6661 agaatttctg aagactataa atatatttat cgggtaaatc aaacatgaaa caaaacatgg
     6721 ggacggttcc ttgtttcaag tacaagcatg aaacaaggaa ccgtccccat gttttgttaa
     6781 ccaagaggcc ttttcaaata aatcatgtcc accaattgct ttctgttctc atatatagga
     6841 tggtcataat tgtctgtaaa aaagttcttt acaacatggg atcttttaaa tccgcatttt
     6901 tcatagaaat ttaaagtcat agggctgtcg cctgttccaa catataactc agtgccagaa
     6961 tctttatagt aatcaataat ataagatacg agatgttgtc cataaccttg tcgttgatat
     7021 ttggggacgg taacgatatt tttaatttca taaatacctg gtccttcctg agtaacaaca
     7081 caaagtgctt taagatcgtc gtcatataat gcgaacatct caccacgata taaatatttt
     7141 tctatcatgt ctacctgttc atctgcaatg agcagcaagt ccatgtattt gcttttatct
     7201 gtatctgaaa ttttctcgac tttcatttgt atgcttctcc ttttattatt ttgttttgaa
     7261 taatatttaa attttaacaa acgtaaatta tattatcaat ctattttttg ggtgaaacaa
     7321 ggaagcgttc tcatgtttca ttttttctct tgatcatatt ccacttatgg aatataatca
     7381 tattaaacaa gtggaatatg aggtggtgaa tatgttaaag catggaatat taggtttgtt
     7441 aaattacggg gatatgactg gatacgaaat tatgaaggtg ttcagggatt cattgaattt
     7501 tttctgggct gcaaagacaa gtcagattta tcgggagttg cagactttga aaaaagaagg
     7561 ttttgtgacc gatgttttgg ttaagcaaac tggaaaatct gacaaaaagg ttttttccat
     7621 tactgagagc ggcaaaaaag aactaaaacg ctggctaagg gaatataatt atggaaatac
     7681 aaataacgcc ttgtgtatga aagtcttttt ttccggtgag ttatccaaag aggaaaatat
     7741 cgaacggttt gaaaacataa aaaaggaagc tcaggaagca ataacaagat attctgctat
     7801 atttgagctt acaaaagctt atagtaagat gctggtcaac cctgaaaaag ctgcttattg
     7861 ggatatgacg gtagattacg gaatacgtta tatgaaaatg ttagtggaat ggtgcgatgc
     7921 ctgtattaga gaattgggag atgtgaaaga atgaaaatac ttattatcaa tggaagccca
     7981 aaaggggatt ccagcaatac tttaaagctt acaaaagcct tcgtggaggg tatgggagat
     8041 catgaaatta aggagattac agtatcaaag ctaaatatat cagcttgtaa gggctgcttt
     8101 tcctgctggg gtaaaagtcc aggaaaatgt ataatagacg atgatatgac tcaggttata
     8161 gaagatgaat tgtgggctga tattattctt tggagctttc ccttatatta ttttagtgta
     8221 cccggttctc ttaagaattt gattgacaga caacttccca tggttctccc ttttatgaca
     8281 gaaagagaag atggtatagg agctggggca catccagcca gatatgatat gagtggcaaa
     8341 agacacgttt tgatttctac ctgtggtttt tattctgccg aaaaaaacta tgacagtgta
     8401 aaaagtatgt ttgactatat tttaggtaaa ggcaattatg agaccatctt ttgtggtcag
     8461 ggagagctat tccgtattcc ggaattaaaa gggagaaccg atgaatatct ccatatcgta
     8521 agaaaagcag gtaaggaata tatctcttca ggaatttcca acacaacaag ggaaaaaata
     8581 aaagaactgc tttatcctaa agaagctttt gaagaaatgg cggatgccag ttggggaatt
     8641 gagaaagagg atggcggaaa agttgaccaa tctttgtctt ttactaaaca aatggccgct
     8701 ttatataata aagaaagtta tgatggtaaa gaccgggttt tagaaatatg ttacacagat
     8761 ttaggaaaga cttatcagat gctattaggg aaagatggct gtcaggtttt tacggatggt
     8821 cgtttaacgg caacaaccag gattgaaacg ccatgggagg tatggctttc tattgccagg
     8881 ggagagataa gaggcgatgt agcccttgca aaaggcatgt ataaagttaa gggtgatttt
     8941 tccttgatga caaattggga taaggttttc ccaagaagca gtcaggtaaa taatgggctg
     9001 tctgatggag actcagaagc aaaaaaacct gccatgctga cgatgctgat accatggatt
     9061 acattttggc tagctgtatc tattaatgcc agttcagggg cagttattac ccttgctgtc
     9121 tgtggctgcc ttccgcttat tatggcgcga aaagccctga ccatttatga taagttttcc
     9181 ctgtcgctta tatctctttt atctgttttg gcgttacagg acaatatgca gataattgct
     9241 attgtggcag cctatttagc atttggttta atgtggcttt tgtcctgctt gacaaaagaa
     9301 cctatttgtg ctgcctatgt caaatacgat tataatgggg acgatgcctt aaacaatcct
     9361 ctttttatga aaaccaatta tatattagca gcatcctggg gtattttata cctcttaata
     9421 gcagtttggt cgtggttttt gctgaatttt aatcagaata tattattaca agtattaaat
     9481 aacgctgcag ttattgcaat ggggattttt acaacctggt ttcaaaggtg gtatccggta
     9541 tatcttgcaa aatgaaacat ggggacgatt cctggaacaa ggaaccatcc ctatgttttt
     9601 gtttcgttta tttacttttt tcactttcgg gatggcataa ggagtaggca ctgcaagcgc
     9661 tgcagccaca tccacatgat gttttccctt tttttctatt ggcaatttgc ttgtagatta
     9721 ttgaagcaac aatcaataaa agtatcacac tgataattat tgtagataaa ttatttatta
     9781 aaaatttaat catattcatt tccccttatc aagatattta ggcactggta attgcctttt
     9841 gtttagccct gctacttgct tgtggtctaa acaggagata tataaatatc gcaagaagag
     9901 caagggctac aattgttccg ggaccaaagc tgccagttat aattaagtta ccaatgttga
     9961 aaatcattaa agatatggaa taagcgaaaa ttgtttgata agcaactgca aacaatgtcc
    10021 atttagcatc attcatttcg cgtcttatag caccaattgc tgcaaaacat ggagcgcaaa
    10081 gcagattaaa tacaaggtag gaatatgcag caagaggcgt aaagctttcc tgcatattag
    10141 cccagatttg ccatccgttt tcagctactt catcaagtcc accaaagagc acgcctaaag
    10201 taccaaccac attttctttg gctatgagac cagttaaagt agcaacggtg ccttgccaat
    10261 gtccccagcc aagtggagca aaaattggag caaggagatt accaaatgtt gcaagtaagc
    10321 tgttattcat ttcaaccatt ccaaagccat tatcatcaaa tccaaatgtc gataaaagcc
    10381 aaactaaaat ggttgccagc ataataaccg tagacgcttt tttaataaag gatgaaccac
    10441 gttcccatgt acttcttaac agattggaaa gtgttggcat gcggtaagca ggtaattcca
    10501 taacgaatgg agctggatcg cctgcaaaaa gctttgtttt tttcaaaatg attccggagc
    10561 aaatgatagc agcaatacca acgaagtaag cactgggaga aacccaccat gccccgccaa
    10621 acattgcacc tgaaataagt gctattattg gaattttagc accgcatggg ataaatgtcg
    10681 tagtaataat ggtcatcttt ctgtcacggt cattttcaat ggtacgtgaa gccatgatac
    10741 ctggcacacc acatcctgaa ccaacaagca ttggaataaa agatttccct gaaagaccaa
    10801 atttcttaaa aattctatcc agaataaagg cgatacgtgc catgtagcca caggattcta
    10861 aaaaagcaag taaaaagaac agcataagca tttgcggtaa gaaacccagt acacttccta
    10921 caccagcaac aataccgtca agaatcaatc cctgcagcca ttctgctgta ttaatttttt
    10981 ctaagaaatt acccactagg acaggaacac ccgggatcca aataccgtaa tctgaaggat
    11041 caggttcttt gatttcgcca atttgtgtat aaatctcgta attaaccata atggtttctt
    11101 ctaaatttcc ttcctcgtca tagaattcag ctggcgcaac aagttcaacg gcttcaacag
    11161 gttcgattcc tgcttcttta gcagcagttt caaaagtttc caggatagta gaagattctt
    11221 cgtattcttc aacagcttcc tggtaagcgg aagagcctat gcccaataag aaccagccat
    11281 caccaaacac accatcgttt gcccagtcgg ttgcaaaggt tccgatagtt gtaacagata
    11341 taaagtaaac caaaaacatg atcactgcaa aaattggcag tgccagccac ctgttagtta
    11401 ctattctgtc gattttgtcg ctggtcgtaa gtttcccttt atttttcttg atatagcagc
    11461 tatcaatgat ggaagctatg tattcgtaac gttcattggt aataatactt tcagaatcat
    11521 catccaggtc tttttcgcaa gcgttaatga gctcttcaat ttcagaaaga acatttgggg
    11581 caatttcaag actttccatt gctttttcat ctctttcaaa gagtttgatg gcaaaaaagc
    11641 gttcctggcc agttggaaga gatacggttt tctgaatttg agccagtgtg tcttcaactt
    11701 tttcagtaaa tttgtgagca ggttgagtgc tggattttgc agcgtttata gctgcatttg
    11761 cagcttcatc aacaccggtg ccttttaagg ctgaaatacc tataacttta cagcctagtt
    11821 tttctgacaa ggccaaaaga tcaattttgt cgccattttt ttcaaccgca tccatcagat
    11881 taactgctac gacaacgggg atacccattt ctgtcagttg tgttgtaaga tataaatttc
    11941 tttcaaggtt ggtaccatca acgatattta atattacatc tggtttttca gtaattaaaa
    12001 agtttcttgc aattacttct tctaatgtgt aaggtgacaa tgaatatatt ccgggaaggt
    12061 caacaacaat gacgtcgtta tgctttttaa gtcttccttc ttttttttct actgtaacac
    12121 caggccagtt accaacgaat tggttagaac cagtcagtgc attaaacaag gtagtcttac
    12181 cactgtttgg attgccggca agagcaattt taattgacat tattcttaat cctccttatt
    12241 aagctttaaa agttagaaca acctaacatt ttgttagaaa aaaatagggc tcatttccct
    12301 cgttaaacat gtaagctatt cattaatcag ttcaacctca attttctcag cgtcttcctt
    12361 tctgatagac agttcatagc cgcggatagt cacttcaatg ggatcaccaa ggggtgcaac
    12421 ttttttaaca tgaagcctag caccttttgt tatacccata tccattagtc ttcttttgac
    12481 agcaccttcg ccatgaaatt tttttacgac aatattttgt ccaattgcaa tatcttttaa
    12541 agtaagcatc attctcatct cctcaaatga cataattaaa ctacaatttt gcatgccatt
    12601 tctttactaa gggcaatgcg agattctttg acattgacga ttaaatttcc atggatatca
    12661 gaaagtacag taacttcagt cccttcaaca aaaccaaggc tttctaaaaa tcgtttaata
    12721 ctgtctttcc cactaatttt tttaatagtt tttctttcac caatctttgc aaatgttaat
    12781 ggcatatacg acactcctaa atatttatta aaaataaaaa ttctataagt tagaaatatc
    12841 taacactttt atattattat attatcacct tttataaaaa tgtcaataga ttctttagaa
    12901 acaaggggat ggttccttgt ttcatttttt tctataaaat aaagtgatgc aaaggtatgc
    12961 cttattttct tcatcatcag aagagaagtt tatatttatt ataaagtaaa taaaaaagag
    13021 atggtgatat gcctcgtcag gcaaggagaa gaagtgaaac aggaggactg ctcctgtttt
    13081 ttgtttgaag gaaatgaaac aaggaaccgt tcctatgttt tattaggcat aattaataga
    13141 attttgatat ttggacaaaa tataataggt aaattaacaa taagcataat tcgacaattt
    13201 atttattttt aataaaaaat ttgttgatta ataaaatttc ctgtgctata atcaacttaa
    13261 catattaaca tataaacata ttaatagatt aacataataa tttacaaaca aaattaattt
    13321 tattaaaaaa gagggctctt taaaaagaaa ggagataata tggaaatatt aaattataag
    13381 caaaagtacg aaaaagaagt ggttgattta tggaacaaaa ctttgacagc agatcctatt
    13441 accattcata agtttagaaa acaaatcctt tttgatgaaa attttgatcc tgaactttgt
    13501 tatctggcag cagaagacga ccgtgtggta ggtttcctgc tttcaacaaa aagaaagttt
    13561 ccttatcttg aaagaggttt ggaacctaca agaggctgga ttaatgtgat gtttgttgat
    13621 caggattaca ggcgaaaagg aattggaaca gctctggtgg aacatgcaga aaagaaattg
    13681 aaatccctgg gagtagagac agtgaccctg gcagcataca gtccgaatta tttcttccct
    13741 gggatagata aggataacta tagtgatgca gtaaaattct ttgaaaaaat gggttatcag
    13801 ccaggcaaag aaagttattc aatgtgcaag gatttacatg gctatcaatt gagtcaggac
    13861 acattagaga aactcaaaaa agcacaagaa gcaggttttt ccttcataaa ctttgaatac
    13921 aagtacgccc tagaattact ggaattttta aaagatgaat ttggtggtgg ctggaagcgg
    13981 aatgcactga tttctatgca aaacaattta gccgaagatt gcattttgct ggtattagac
    14041 tcccaggaaa aaattgtagg cttttgcatg agaatgattg atggtaaccc catgcgtttt
    14101 ggtccgattg gtgtcaagaa agaagccaga aactatggca taggcggtat tttatttgat
    14161 ctgatgcaat ttgaaatggc taagcgcggt atattccact tattcttctt atcaaccgat
    14221 gaacctggca gaagatttta tgaaagacac ggtgttaagg tctatagaac ctttgttgat
    14281 tatcaaaaga caatatgacg ataatagtaa aggaggagac taataaatga gtgaaatcaa
    14341 acgcgtagtc agtgttggag cacattctct ggatgcggag attttaggcg gacccgttat
    14401 gctaaaatat gcaaaacaag gcgctcactg tacttatgtt catgtgaccc agggccgtct
    14461 ggaagatcca aatgcaacgg aagaagaaaa gcaagcctat ttaaaagaat tattaaacca
    14521 aaacaagcgg gctgctgaaa agttaaacgg tgattgtctt tggttaggct atgtttccag
    14581 taatatgcct tctctagaag aatttgcttc cagaatggag caatatttca tagatgaaaa
    14641 agtcgatcta gttattacgc attggcgcgg ttccatgcat ccccgacata ttaatacgtc
    14701 cgatgcagtg actactgcag taaaatgttt gagggaaaag ggaaatccta ttcgtttagt
    14761 atacggagaa aatttcgagg atttggttgg ttttattcca caagcttatt ttaaattgga
    14821 gccggaagaa gtggaacaat ggttttcagc cctacgcgaa tactccgtat tttgcggaaa
    14881 agtgaatgac tttccatata atgattacta tacaactata gggaaagttc
//
LOCUS       DAOSUD010000053        14634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2484_length_14634_cov_24.914329, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000053 DAOSUD010000000
VERSION     DAOSUD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 14634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14634
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2484_length_14634_cov_24.914329"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..889
                     /locus_tag="PLG49_07585"
     CDS             302..889
                     /locus_tag="PLG49_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ17219.1"
                     /translation="MKILIVGDKESSYIWDHFDRERFKDIDLTLSSGDLKAEYLSFLV
                     TMIKAPLFYVHGNHDGNYMTNEPLGCVNIDDKIVVHNNIRILGLGGSQAYNFGPFQYT
                     EKDMQTRVKKLRRTIKKHNGFDILLSHAPAFGLGDGDDLCHRGFQVFLELLDEYKPKY
                     FIHGHQHLNYGRQKRYLTYNETTIINAYEYHIFEY"
     gene            complement(925..2889)
                     /locus_tag="PLG49_07590"
     CDS             complement(925..2889)
                     /locus_tag="PLG49_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ17220.1"
                     /translation="MEDLQSVYQHAYKLGKANYSENVSKGLSGYLPSLDGIVKNNEIS
                     SEINLGLVEIPLKKIVGTYSHSRSIAFAKNFMPLIAGESEFKAKWIRLYESHLEEGIR
                     DPIKVYEYLNWFYVVEGNKRVSVLKYCDAYSISAFVTRLLPKKDENDLVNKIYYEFLE
                     FNKLTGINEIWFSKENSFNELSKYIQEYNPELKIYTDKYKHFLGNVYRPFREIYHKLG
                     GDELKITTGDAFLEYIKIYGIPDEIEEEKCRQRIKKLMIELEALSDEDPVEIRTDTIE
                     VPKKGVISSITHHFMAPKKLKIAFVYAKTIKSSGWTYAHNLGRLHVENVLKDQISTTY
                     IENVPENNNAYKYIKDLAKKDYDIIFTTSPTYIIPTLKAAFEFPDVKFLNCSATHSFK
                     HVCTYYGRIHEPRFLTGIVAGALTKTNIIGYVATYPISEVISSINAFTLGARLVNPYV
                     KVKVVWTKKWDQPEKSKNLAGTLKDYGADIIAHDDMPVPGDDSKEYGVYATKCNEEGD
                     HCIPQAHFAMPIWNWGVFYERLLRNVLSGTWRVVFDVLDSNPKLVNFWWGMDTGMVDI
                     IYSRTHVPLETQRLIEFMRKMIIQNEYHPFTGPIYDQEGNLKIKADDCASYEQILSMN
                     WFVEGVEGEIPKLDDDQFADPLTEMLGIKK"
     gene            complement(3071..3358)
                     /locus_tag="PLG49_07595"
     CDS             complement(3071..3358)
                     /locus_tag="PLG49_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17221.1"
                     /translation="MLLKKNSPAHLEEIKAMVRLLDKKKKKKMIGFIVLAVALLLAVT
                     GIIFWLMNRNKDDDFEDDYDYDDYDDYNVEGTYPNGVYDEDIEDEIDESNE"
     gene            complement(3443..3528)
                     /locus_tag="PLG49_07600"
     tRNA            complement(3443..3528)
                     /locus_tag="PLG49_07600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3491..3493),aa:Ser,seq:cga)
     gene            complement(3683..5785)
                     /locus_tag="PLG49_07605"
     CDS             complement(3683..5785)
                     /locus_tag="PLG49_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOQ17222.1"
                     /translation="MSLMLDFFSMDSSQKFMTYMATVKDYLIADAVFSDETANFRNPV
                     EPKSSESVTVKIRTAKDNCDEVFLIYEDKKIKMEKCMQDELFDYYQGLIPPVSHTLSY
                     HFELKKNEISYYYNKKGLLLEKDPFFDFKIIPDFSTPAWAKGAVMYQIFVDRFYNGDP
                     TNDVLDNEYIYAGKPSQQVKDWYKYPEVDGIREFYGGDLKGVIDKLVYLKELGVEAIY
                     FNPIFVSPSNHKYDIQDYDYIDPHFGVIVEDGGELLSPGKFHNKYASKYIKRTTDLKN
                     LEASNALMIELINKAHEYGIKVILDGVFNHCGSFNKWMDREGFYEGLADYQGGAYRSK
                     ESPYHNYFSWYHHNWPNNDCYHTWNGFDTLPKLNYEGSQELFNHIINIGRKWVSPPYN
                     ADGWRLDVAAELGYSKEFNHHFWREFRKAVKEANPNAIILAEHYGDSYDWIQGDQWDT
                     VMNYDGFMDPVSYFLTGIQKHSDHYREDLLGNSYVFKDAMKYHMSRFSYQSLYTAMIQ
                     LSNHDHSRFLTRTNRTVGRIHTMGPEAASQNINKGIMKMGVVIQMTWPGAPTIYYGDE
                     AGLAGWTDPDNRRTYPWGREDQELLNFHKEIIRIHKSYQALKTGSLEFLLTDYKVLSY
                     GRWDMEHAFVIALNMDDVERELFIPVWKIGISNTVNLERIFFSDKETHSTQSVQHEVS
                     NGHLCIKMPPYSSMIFIN"
     gene            5999..6418
                     /locus_tag="PLG49_07610"
     CDS             5999..6418
                     /locus_tag="PLG49_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17223.1"
                     /translation="MADGFASCGNLPALLSNYIGETVTIFTSSGGQSGAGFTGVILSV
                     NNVFVRLITRIGPPPGCSLGNACTGFKVGYGWDGYDNGCGITGAYSNGYDAGPIAPAS
                     EIGALGSGGWDGYPVYTVGSVTDIPIDRIVSFVHNAV"
     gene            complement(6501..6755)
                     /locus_tag="PLG49_07615"
     CDS             complement(6501..6755)
                     /locus_tag="PLG49_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17224.1"
                     /translation="MQYVTEYAKRRNTVKLSLNTKKKKMVQMVYRQPDNKLIKRLSYT
                     SPNLEEIVLFYFRQQNKYRKMGYEIKEKKDGKIITPVFKK"
     gene            complement(6851..7288)
                     /locus_tag="PLG49_07620"
     CDS             complement(6851..7288)
                     /locus_tag="PLG49_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17225.1"
                     /translation="MISLAGRAAEELIFGEDNITTGASNDIEKATQIIRDYVVKYGMS
                     KSLGLLNIDVLFNNQSQAFSEEALIKECSKHMERLYKETRNLIIEHKYYLNQIAKGLL
                     YKESLSEEDLDDIMEDKFSISDSVNEKEDLLPMVKCNRVVEAR"
     gene            complement(7281..8684)
                     /locus_tag="PLG49_07625"
     CDS             complement(7281..8684)
                     /locus_tag="PLG49_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOQ17226.1"
                     /translation="MNKNKIFKWALPFIFLAVSLSFLFFYQRSKIEEMTYYEFLEKVE
                     SNQVEEVILNSNPKMKVRLKEGSDKIYVTDNPRNPELKEFLLKQQIQVVEDDSATFSN
                     ILRGVFMIGIFFAFLRYMSKNFGQQSAKDMMNIQTSALPGPNKTYSFNDVAGNEEAKE
                     EVKDIIDFLKKPEKYIKYGARMPRGIIFYGPPGTGKTLMAKAIAGEAGVPFFSVSGSD
                     FVQMYVGVGASRIRSLFEKARKAGKALIFIDEIDALGKKRAASVTGGNDERDQTLNAL
                     LTEMSGFKENEGIIVIAATNRLDVLDEALLRPGHFDRQVEIALPDVKGREEILRLHGK
                     NKPLSDEVDFSKLAKQTVYFSGAMLENLLNEAAIMAANRNAKAISLKDIDKAFYKIIA
                     GSEKKDRSNILEKDREITAFHEAGHALVTKLIAPGHTVSKVTIIPSTKGAGGFSMNIS
                     PDKMYHTKKRYGSSDYD"
     gene            complement(8853..9560)
                     /locus_tag="PLG49_07630"
     CDS             complement(8853..9560)
                     /locus_tag="PLG49_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17227.1"
                     /translation="MDKQKETVVPSFAISLEEVEERMNLLQEFIKNHMKKGEDYGEIP
                     GITKPTLLKSGAEKLCDVYGFSKLVEVKNRVEDWEKGFFHYEVEVSLINKKTGLIEAQ
                     GVGSCNSKERKYRYQDAYTIVNTILKMAKKRALVDAVLSATRSSGIFTQDVEDMEADG
                     AEEIEKPSTERQRRYILSLTKEKHFDEDQLRTFCSNVIGKDKPSKDWTSEEASKIIEA
                     LQNTPKSSIIVNSDKKR"
     gene            complement(9914..10300)
                     /locus_tag="PLG49_07635"
     CDS             complement(9914..10300)
                     /locus_tag="PLG49_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17228.1"
                     /translation="MSNAKYLYELSEQDGIYSKAYNQFGDNLHRRLDMIKGYPVNSAG
                     DNPLYLLQLVTYGEKVSEDMNEGIAIKPYELTDLLKAFVCAVEHLPADIILTKPGVCK
                     YIQSHIEEFLSDILKENAQKVSSRSL"
     gene            complement(10413..12890)
                     /locus_tag="PLG49_07640"
     CDS             complement(10413..12890)
                     /locus_tag="PLG49_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ17229.1"
                     /translation="MFDSNYQKFIKFITNRLFILSLCFLVMFTILGVHLFKLQIIEGE
                     YHQSKVNTNILRELPISAPRGTIYDRYGRPLAVNKLAMSIKLDPSIDVENRDEVLLNL
                     IELLEKNNEKLIDNFPLSLDRPFEFQSSKNEELNLKAEIFKTTKDNLTEEQKNMNGEE
                     FFKELRKFFEIDESLNDSDARKLISLKYALYEKRYRKYQPITIATDIKDKTIAHLVEN
                     YESYPGFYIDVESVRYYPEGELVSHILGYTGRITEEQYEELKDEGYHPDDIVGIVGIE
                     KEMEAYLRGKDGSISVEVDPFGQRKEVHNTEDPIPGGKVFLTLDVGFQQKVEDILVGK
                     LRDIILSRIERSPTKSEPPFYIQDLLSGLVKTNTISIEKILEAPVGTAQYELAQILKS
                     KMSQSEIKNDEEYNEIAHAKELLLEIIKNKEVSSKVFIAIMVEQEVISADEEFKQKVE
                     NGNISPIQVLRERIENGEIMPHELGVDPSTGAVVVVDIHTGEVLSLVSYPSYDNNKFI
                     NGIDYEYYKKLQQDPTIPMFPRATQGRMAPGSTFKMVTGIAALEEGIVTPRDKINDKG
                     LYKDAGLPYARCWIYSNYNIGHGYTDIHRAIEVSCNYYFYDVSYRMARIDAQNSIEQN
                     GLNKLRYYASKFGLDSKTGIEVPEYEPHFSDKDVIRSAIGQGTHNFAPVHMARYIATL
                     ANGGTLYKFHIVDSVKTFDGDIILKKEPEIQEQSNFKPENLDAIYKGMHLVTAGNEGT
                     VRNIFKDFPIEVAGKTGTAQEQLSRGAHAWFVGFAPYEDPQIAIAVAIPYGYSSNYPA
                     EIFRDIVAEYFGLNADSEKATIDNVLD"
     gene            complement(13055..13330)
                     /locus_tag="PLG49_07645"
     CDS             complement(13055..13330)
                     /locus_tag="PLG49_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HOQ17230.1"
                     /translation="MTLAETQEHLRFYDSNARGKIEKLASKEFEKMCMETFEKYKEKG
                     IDLFRIEEMFSRKYPNIKIKNPIRITNLKLNVNVHLEGSTDVLSYRE"
     gene            complement(13327..14169)
                     /locus_tag="PLG49_07650"
     CDS             complement(13327..14169)
                     /locus_tag="PLG49_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOQ17231.1"
                     /translation="MKIKMLVIFLINLIILTGCFGYRDMDKALFCTAIIIDVDENENV
                     LLYTELFHSYRSKKDNSETGTRIIFTSKGKNLFEAIRDQNKSTSYKINYEQNKIIIFT
                     TRAAQYGIDNFIDYLNRDQELLLRQYLLIFEGDPRSLIQLKVRQEEFVGLYIYDLVRN
                     EITKNFADIYQIYEYLNDRYVGNSTSVIAMIKLKDETLQEDLVIEGSGIIKDGKMIDK
                     MEAKDSATHSALVGNESVKIISVPHPYHKESRIILEILDSNLTTNLSYEDNEIILEKI
                     LTLR"
     gene            complement(14166..>14634)
                     /locus_tag="PLG49_07655"
     CDS             complement(14166..>14634)
                     /locus_tag="PLG49_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOQ17232.1"
                     /translation="ARAGVPFPIYLEVLLMMFFFQILREASLRLPQSIAQAISIVGAL
                     ILGDTAVQSGLASTIAILLVALTALSAFLAPNIFGAISIWTDILIIFSSLLGLPGFLI
                     GLLLLISHLASLTSCGYPFLYPVGTKDSVQYSDRILRPEIHHLPDSINEDDQK"
BASE COUNT         4554 a         2808 c         2088 g         5184 t
ORIGIN      
        1 tcctattcta catgattttt tcacaggggt gttgcattta atattgcaat ttaagatatt
       61 atttttggtt aaattaattt ttttattcga catttataac aatacttata ttggctttta
      121 tgcccgataa ttctgccttt ttacttaatt atcctatacg tttctttttt atgaatttca
      181 cttttgagac ttattcattt tatattttta tgttcgcttg ttttatattt tgttatttta
      241 tgatacacta ttatacaaac aacaaaagta aattttcttt atagaaagtt ggaagagttc
      301 aatgaaaata ttgattgtcg gagataaaga atcttcttat atatgggatc atttcgacag
      361 ggaacggttt aaagatattg atttaacgct ttccagcggg gatttaaaag ctgaatacct
      421 ttcctttttg gtaaccatga tcaaggcacc actattttat gttcatggaa accatgatgg
      481 caattatatg accaatgaac ctttaggctg tgtcaatatt gatgataaaa ttgttgtcca
      541 caataatatc cggatccttg gtctgggtgg ttcccaggct tataatttcg gcccttttca
      601 atatacagag aaagatatgc aaacccgcgt aaaaaaatta agaagaacca tcaaaaaaca
      661 taatggcttt gatattcttt taagtcatgc cccggctttt ggcctggggg acggggacga
      721 tttatgtcac agaggttttc aggtttttct tgaacttctg gatgaatata agccaaaata
      781 ttttattcac ggtcatcagc atttaaacta tggtcgtcaa aaaagatatc tgacttacaa
      841 tgaaacaacc attattaatg cttatgaata tcatattttt gaatattaat ccatttgctt
      901 aaaaaaacag ccgtccattg acggctattt ttttattcca agcatttccg ttaaagggtc
      961 tgcaaattgg tcatcatcta atttgggaat ttccccttct accccttcta caaaccagtt
     1021 catagacaag atttgttcat aactggcaca atcatctgct tttattttaa gatttccttc
     1081 ctggtcgtat attggaccag taaaaggatg atattcattt tgaataatca ttttcctcat
     1141 gaattctatt aatctttgtg tttcaagggg tacatgggtt ctggaataaa taatgtccac
     1201 cattccagta tccatacccc accagaaatt aaccagttta ggattggaat ctaagacatc
     1261 aaatacaact ctccatgttc cgcttaaaac atttctaagc agtctttcat agaaaacacc
     1321 ccaattccag ataggcatag caaaatgagc ctgaggaata caatgatctc cctcttcatt
     1381 gcattttgtt gcatatacac catattcttt agaatcatcg ccaggaactg gcatgtcatc
     1441 atgagcaata atatctgcgc cataatcttt tagggtgcct gccaggtttt ttgatttttc
     1501 aggttgatcc cattttttag tccaaacgac tttcactttt acataaggat taaccagtct
     1561 tgctccaagc gtaaaagcat taatgcttga aatcacctct gaaatgggat aagttgctac
     1621 ataaccaata atatttgttt tggtcagggc acctgctaca atacccgtta gaaatctcgg
     1681 ctcatgaata cgaccataat aggtacatac atgtttaaac gaatgggtcg ctgagcagtt
     1741 taaaaactta acatcaggga attcaaaagc tgctttcaaa gtcggtatga tgtatgtagg
     1801 gctggtggta aaaataatgt cataatcctt tttagctaaa tctttaatat atttataggc
     1861 attgttattt tccggaacat tttcaatata agttgtgctg atttgatctt ttaaaacatt
     1921 ttcaacatga agccttccta aattatgggc ataagtccag cctgaactct taatggtttt
     1981 tgcatataca aaagcaattt taagcttttt aggtgccata aaatgatgcg taatggaaga
     2041 aataacccct tttttaggaa cttctatggt gtcagtcctg atttcaaccg ggtcttcatc
     2101 agataaagct tcaagctcta tcattaattt tttgattctt tgcctgcatt tttcttcttc
     2161 aatttcatca ggaataccgt aaatcttaat atattctaaa aaagcatctc ctgttgttat
     2221 ctttaactca tcccccccta atttatggta aatttcccta aaggggcggt aaacatttcc
     2281 taagaaatgc ttatatttat ctgtataaat tttaagctcg ggattatatt cctggatata
     2341 tttacttaat tcattaaaac tgttttcctt agaaaaccaa atctcattaa tccctgttaa
     2401 tttattaaac tcaaggaatt cgtaataaat cttattgact aaatcatttt catctttttt
     2461 aggcaacagc cttgttacaa aagcagaaat actataggca tcacaatatt ttaatacact
     2521 aaccctttta tttccttcaa ccacatagaa ccagttcaga tattcataaa ctttaattgg
     2581 gtctctgata ccttcttcca gatgtgattc ataaagtcgt atccactttg ccttaaattc
     2641 tgattctccg gcaatcaaag gcatgaaatt ttttgcaaaa gcaatgcttc ttgaatgaga
     2701 ataagtccca actatttttt taagtgggat ttctaccaga cccaaattaa tttcagatga
     2761 gatttcatta ttttttacta tcccatctaa agaaggtaaa taaccagaaa gaccctttga
     2821 aacattttca gaataatttg ctttacctaa tttataggca tgctgataaa ccgattgcaa
     2881 atcttccatc actatacccc ccaccccata aaagcctctg ggaaaaaatt taattattaa
     2941 gactacttgt taccattata ctattttttt gtaattttgc tagtattctt ttgataaaat
     3001 aatcctataa aaaaaagaca tctgagatgt cttttttact ttgtattata atttctactg
     3061 ttttatcact ttattcgtta ctttcatcta tttcatcttc aatatcttca tcgtatacac
     3121 catttggata tgtgccttct acattgtaat catcataatc gtcataatca tagtcgtctt
     3181 caaaatcatc atctttattc ctgttcatta gccagaaaat aataccagtt actgctaata
     3241 aaagagcaac tgcaagaaca ataaatccta tcattttttt cttctttttc ttatcaagca
     3301 aacgaaccat agctttaatt tcttcaagat gtgcggggct atttttcttc aacaacatat
     3361 tatcctcctt tgacttctac aaaataggaa ctatatataa taatatacca taaaatcata
     3421 aaaaataata caaaaagtta aacggagtgg gtgggattcg aacccacgga accctgaggt
     3481 tcaactgatt tcgagtcagt ctcgttatga ccacttcgat accactccat aataagaagt
     3541 tagaaattta aacttataag ttgaaaacaa aagaagtcaa aagaggaaaa tataaaaagt
     3601 aagttcagac taacatatga aatattatta tatatttttt ttattgtcaa gtagacaaat
     3661 atttgaatca gcttgcaaaa tcctagttta taaaaatcat actgctgtag ggaggcattt
     3721 taatacacaa atgcccatta gaaacttcgt gttgaacgct ttgtgttgaa tgtgtttctt
     3781 tgtcactaaa aaagattctt tccaagttga ctgtattgga aattcctatt ttccatactg
     3841 gtatgaaaag ttccctttct acatcatcca tattaagggc aataacaaaa gcatgctcca
     3901 tatcccatct gccatagctt agtaccttat aatctgtcaa taagaattct aaagaacctg
     3961 tctttaaggc ctgatagctt ttatgaattc tgataatctc tttatgaaaa tttaagagtt
     4021 cctggtcttc cctgccccat gggtaagttc ttctgttatc agggtctgtc caacctgcaa
     4081 gtcctgcttc atcgccataa taaatggttg gtgcacctgg ccaggtcatt tgaataacaa
     4141 cacccatttt cataatgcct ttattaatat tttggctggc tgcttccgga cccatggtat
     4201 gaatgcggcc tactgtacgg tttgttcttg ttaaaaatct tgaatgatcg tgatttgaca
     4261 attgaatcat ggccgtataa agggattggt aactaaacct tgacatatga tatttcatgg
     4321 catctttaaa aacataactg ttgcctaaaa gatcttctct gtaatgatcg ctgtgttttt
     4381 gtatacctgt taaaaaataa gaaaccgggt ccataaatcc gtcataattc atgactgtat
     4441 cccattgatc gccctgaatc caatcatagg aatccccata gtgctcagcc agtataatag
     4501 cattgggatt ggcttcttta actgctttcc tgaattctct ccaaaagtga tgattaaatt
     4561 ctttactata gcctaattct gcagcaacat ccaatctcca gccatctgca ttataagggg
     4621 gtgataccca cttacgacca atattaataa tatgattaaa aagttcctgg ctaccttcat
     4681 aatttaactt aggcaaagta tcaaaaccat tccaggtatg ataacaatcg ttattgggcc
     4741 aattgtgatg ataccaggaa aaataattgt gataggggct ttctttactt ctgtaagcgc
     4801 ctccctggta atccgcaagt ccttcataaa agccttctct gtccatccat ttgttaaagg
     4861 aaccgcagtg attaaaaacc ccgtctaaaa tgacttttat gccatattca tgcgccttat
     4921 tgattaattc aatcattaag gcattgctgg cttctaaatt cttaagatcc gtcgttcttt
     4981 ttatatattt actcgcatat ttattatgga actttcccgg gcttaacagc tccccaccat
     5041 cttctacaat cactccaaag tgcgggtcaa tatagtcata gtcctggata tcatatttat
     5101 gattactggg agatacaaat attggattaa agtaaatcgc ttctacacca agctctttta
     5161 aataaaccaa tttatcaata acacctttta aatctcctcc ataaaattcc cgtatcccat
     5221 caacttcagg atatttatac caatctttta cttgctgtga gggcttaccg gcataaatat
     5281 attcattatc aaggacatcg ttcgttgggt ccccgttata aaacctatcc acaaaaattt
     5341 gatacattac tgcgcctttt gcccatgctg gcgtagaaaa atcaggaatt attttaaaat
     5401 caaagaaagg atctttttct aaaagcagac cttttttatt gtaatagtag cttatttcat
     5461 ttttctttaa ttcaaaatga tatgaaagcg tatgactaac tgggggaata agcccctgat
     5521 aataatcaaa taattcatct tgcatacact tttccatttt tattttttta tcttcataaa
     5581 taagaaaaac ttcatcacaa ttatctttgg ctgttcttat tttaaccgta accgattcac
     5641 tgctttttgg ctccacagga tttctgaaat tagctgtttc atcactaaat acagcatctg
     5701 caataagata atctttaacg gttgccatat aggtcatgaa tttttgacta gaatccatgg
     5761 agaaaaaatc aagcataagg ctcataaata acctcctcgt actattgcct attctgttcc
     5821 ctggtccgca tcctgttttc tcattattat atcatattct aaaaaatctt aaaatctttc
     5881 atcaaataat taaaaattat tatagacatt tagtcacact taattcattg acttaataat
     5941 atatattaaa ggttctcgcc taagcttttt ggcgcttact ttagaaagga ggtaaattat
     6001 ggctgacggc tttgcttcct gtggaaatct tcctgcctta ttatccaatt atattggaga
     6061 aacagttacg atatttacca gtagcggtgg tcaatccgga gcaggtttta ctggtgtaat
     6121 tctcagtgta aataatgtat tcgtccgctt aatcacccgc atcggacctc cccctggatg
     6181 ttcattaggt aatgcatgta ctggatttaa agtaggttat ggctgggatg gctatgataa
     6241 tggctgtggt attacaggcg cttacagcaa tggatatgat gcaggaccta ttgctccagc
     6301 aagcgaaatt ggtgcccttg gttccggcgg atgggacgga tatcccgtat atacagtagg
     6361 ctctgtaacc gatataccaa ttgatagaat cgtatccttt gttcacaatg ctgtttaatt
     6421 ttaaaagcgg ttcctaaaga gccgctttta aaaattatgt aaatctatta ttatcaagta
     6481 tccaggaagt atttcatgcc ttatttttta aaaacaggtg ttatgatttt cccatctttc
     6541 ttttccttta tttcatatcc cattttacga tatttgtttt gctgcctgaa ataaaataaa
     6601 acaatttctt caagattggg actggtgtaa gaaagtcttt tgattaattt attatctggt
     6661 tgcctgtaaa ccatttgcac catctttttt ttcttagtat ttaatgataa cttcactgta
     6721 ttccttcttt tagcatattc agtgacatat tgcatgacaa cacctcctat gctatagtat
     6781 gtaaatcgga taacaattat tcacagcaga ttacggctat aaaaaaacgg gtacacaaag
     6841 gtacccgttt ttatctggct tcaaccactc tattgcattt aaccattggc agtaaatctt
     6901 ctttttcatt cacagaatca gaaattgaaa atttatcttc cataatatca tccaaatctt
     6961 cttcactgag agattcttta tataaaagac ccttagcaat ctgatttaaa taatatttat
     7021 gttctataat aagattcctg gtttctttat atagtctttc catatgtttg ctgcattctt
     7081 ttattaaagc ctcttcagaa aaagcctggg actgattatt aaataagaca tctatattaa
     7141 gcagacctaa gctcttactc atgccgtatt ttacaacata gtccctgata atctgagtgg
     7201 ctttttctat atcattactt gcacccgttg taatattatc ttcaccaaaa atcaattctt
     7261 ctgctgccct gcctgccaga ctaatcataa tctgaacttc catatctttt ttttgtatga
     7321 tacatcttat caggagaaat attcatacta aaacctcctg caccctttgt acttggaata
     7381 atcgttactt ttgaaaccgt atgacctggt gctattaatt tggtcaccag ggcatgacct
     7441 gcttcatgga aagctgtaat ttctctgtct ttttctaaaa tattgcttct gtcctttttt
     7501 tctgaacctg caataatttt ataaaaggct ttatcgatat cttttaggga aatcgctttc
     7561 gcattcctgt tggctgccat aatagccgct tcatttagta aattttcaag catggcacca
     7621 ctaaaataaa ccgtttgttt agccagcttc gaaaaatcca cttcatcaga aagaggttta
     7681 tttttcccat gaagccttaa aatttcctct cttcctttta catcaggaag ggctatttcg
     7741 acctgtctgt caaaatgtcc tggtctaagc agggcttcat ctaaaacgtc caaacgattg
     7801 gtagctgcaa tgacaataat tccttcattc tctttaaaac cagacatttc ggttaaaaga
     7861 gcatttaaag tttgatctct ttcatcattt ccccctgtaa cactggcagc tctttttttc
     7921 cctaatgcat caatttcatc aataaaaatc aaagctttcc ccgccttacg ggctttttca
     7981 aaaagactcc tgattctgct tgccccaacg ccaacataca tttgaacaaa atctgaaccc
     8041 gatacagaaa agaaaggaac ccctgcttcc ccagctatgg cttttgccat taaagtcttc
     8101 cccgtcccgg gagggccata aaaaataatt ccccttggca ttctggctcc atactttata
     8161 tacttttcag gttttttcag aaaatcaatt atatctttta cttcttcctt tgcttcttca
     8221 ttacctgcaa cgtcattaaa agaataggtc ttattaggtc caggaagagc gcttgtttga
     8281 atattcatca tatcttttgc actttgctga ccaaaatttt tactcatata tcttaaaaat
     8341 gcaaaaaata tgcctatcat gaaaactccc ctgagaatat tagaaaaagt tgctgaatca
     8401 tcttctacaa cctgaatttg ttgttttaat aaaaattctt ttaattcagg attccttgga
     8461 ttatctgtaa cgtatatttt atctgaaccc tcttttaacc ttaccttcat ctttggattt
     8521 gaattaagga taacttcttc cacctggttg gattccactt tttctaaaaa ttcataataa
     8581 gtcatttctt caattttaga acgttgataa aaaaataaaa aactaaggct tacagccaaa
     8641 aaaataaaag gcaaagccca tttaaaaatt ttattcttat tcacatttat ccccctaaca
     8701 agctagagcg cttatagttt acataatttg tttacattat aatacatttt ttcaaaattg
     8761 tctatggcag ttatttcata atgtctggta aaaacaaaga gggtcctctt tttaattatt
     8821 tcgcataaaa aataacagca ataaatgctg ccttatcttt ttttatcaga attgacgata
     8881 attgaagatt ttggtgtatt ctgcagtgct tctataattt tacttgcttc ttcactggtc
     8941 cagtctttac tgggtttatc ttttccaatc acattagaac agaaagttct taactgatct
     9001 tcatcaaaat gtttttcctt cgttaaactt aagatatatc ttctctgtct ttctgtacta
     9061 ggcttttcta tttcttctgc tccgtctgcc tccatatcct ctacatcctg tgtaaaaatt
     9121 cctgaagaac gggttgcaga caaaacggca tccactaaag cccgcttttt tgccatttta
     9181 agaattgtat taacaatcgt ataagcatcc tgatatctat attttctttc ctttgaatta
     9241 caagaaccta ccccctgagc ttcaattaag cctgtttttt tattaatcag acttacttcc
     9301 acttcataat gaaaaaatcc cttttcccaa tcctcaactc tgtttttgac ttctaccaat
     9361 tttgaaaatc cataaacatc acaaagtttt tctgcccctg attttaatag agtcggtttt
     9421 gtaattccag gaatttcacc ataatcttca ccttttttca tatgattttt tataaattct
     9481 tgcaaaagat tcattctttc ttctacttct tccaaagaaa ttgcaaaaga aggtacaaca
     9541 gtctcttttt gtttatccat gttaaatttt gaccctccca ttccttagat tatctttata
     9601 atttgtaaaa aaacataaaa atatgtaaaa tgagtaaagc aaattataga aaaaaagaaa
     9661 acagtggatt ccactgtttt ctggagaagg tgttaaatat tttgagggtg cagcagccta
     9721 ttgctgctta atttatctca aatcaattgg ggggtcttac ggtttttatt atagcaaaat
     9781 tttaaatgta agtgtgcata aacatattta tgtttttctt tcattttcgt atattttacc
     9841 taatacaaaa agaataatat tttatgtcaa aaaaaatatt atacaaaaaa attggtcgaa
     9901 aaagtctttt atcttataag gaccttgatg acactttttg cgcattttct tttaatatgt
     9961 cagaaaggaa ttcttcaata tgtgattgaa tatatttgca tactccaggt tttgtcaaaa
    10021 taatatctgc tggtaaatgt tcaactgcac atacaaaagc ttttaataaa tctgtcagtt
    10081 cataaggctt tattgctatt ccttcgttca tatcttctga cactttttcg ccatatgtta
    10141 caagctgcag caaatataaa ggattatcgc cagcactatt gacagggtag cctttaatca
    10201 tatcaagccg tctgtgtaag ttgtccccaa attgattata tgccttactg taaataccgt
    10261 cctgttctga taattcataa aggtattttg cattagacat ccatgctcct ccttatataa
    10321 ggtttgtata tttacctaca gtaaattata ttaaacgacc aggaattata ttactaagga
    10381 ggagaaatat actgtatgga ccatttattc atttaatcga ggacattatc aatggttgcc
    10441 ttttctgaat ctgcatttaa gccaaaatat tcagctacaa tatccctgaa tatctctgca
    10501 gggtaattag aagaatagcc ataaggaata gctacagcaa ttgcaatctg cggatcttca
    10561 taaggcgcaa agcctacaaa ccaggcatgg gcacctctgc ttaactgttc ctgggctgtt
    10621 cctgtttttc ctgccacttc aattggaaaa tctttaaaaa tatttcttac agtcccttca
    10681 tttcccgcag taaccaaatg cataccctta taaatggcgt caagattttc aggtttaaaa
    10741 tttgattgtt cctgtatttc gggttctttt ttaagaataa tatctccatc aaaagtttta
    10801 accgaatcga caatatggaa tttatataaa gtgcctccat tagccagggt tgcaatatat
    10861 cttgccatat gaaccggagc aaaattgtgt gttccttgtc caatcgctga acgaataacg
    10921 tctttgtcag aaaagtgagg ttcatattca ggtacttcaa tgcctgtttt agaatctaaa
    10981 ccaaatttac ttgcatagta acgcagttta tttaaaccat tttgttcaat actgttttgc
    11041 gcgtctattc tggccattct ataagacaca tcataaaagt aatagttaca ggatacttca
    11101 atggccctgt gtatatccgt ataaccatgc ccaatattat agttactata tatccagcaa
    11161 cgtgcataag gtaagcctgc atctttatat aagcctttgt cgttaatctt atctctgggt
    11221 gtaacaatac cttcttctaa agccgctata cccgtcacca ttttaaaggt agaaccaggc
    11281 gccattcttc cctgagtcgc cctggggaac ataggaatcg tggggtcttg ctgaagcttt
    11341 ttatagtatt cgtaatcaat tccattaata aacttattat tatcataact tggatagctc
    11401 acaagactaa gaacttctcc tgtatgaatg tcaaccacaa caactgctcc tgtactgggg
    11461 tcaacaccca attcatgagg cataatttcc ccattttcta ttctttccct taacacttga
    11521 ataggtgaaa tatttccgtt ttctactttt tgtttaaatt cttcatcggc cgatatcact
    11581 tcttgctcaa ccataatagc aataaatact tttgaagaaa cttccttgtt ttttataatc
    11641 tccagaagca attctttagc atgggcaatt tcattgtatt cttcatcgtt ttttatttca
    11701 ctctgactca ttttactttt caatatttga gccagttcat attgcgccgt accaacaggt
    11761 gcttcaagaa ttttttcaat cgaaattgta ttggttttga ctaagcctga aagtaaatcc
    11821 tgaatataaa aaggcggctc actttttgta ggagagcgtt caattctcga aagaataata
    11881 tcccttagtt tcccaactaa aatatcttca accttttgct gaaaaccaac atctaaagtt
    11941 aaaaaaacct tgccaccagg tatcggatct tccgtattgt gaacttcttt tctttgacca
    12001 aaggggtcta cttctaccga tatactgccg tcttttcctc ttaaataagc ttccatttct
    12061 ttttcaatcc caacaatgcc tacaatatca tctggatgat aaccttcatc ctttaattcc
    12121 tcatactgtt cttctgttat tcttcctgta tagcccaaaa tatgactaac caattctcct
    12181 tcaggataat accggactga ctcaacatcg atataaaaac caggatagct ttcataattt
    12241 tctactaaat gtgcaatggt tttatccttt atatccgttg ctatggtgat tggctgatat
    12301 tttctatatc tcttttcata aagagcatat tttaaagaaa taagctttct ggcatcgctg
    12361 tcatttaaac tttcatcaat ttcaaaaaac tttcttaatt ccttaaagaa ttcttccccg
    12421 ttcatatttt tttgctcttc tgttaaattg tctttggtag ttttaaaaat ttcagctttt
    12481 aaatttaact cttcattttt tgacgattga aattcaaagg gtctgtctaa agataaaggg
    12541 aaattatcaa tcagtttttc attatttttt tcaaggagtt caattaaatt taataaaact
    12601 tcatctcgat tttccacatc aatactagga tccaacttga tactcatagc taacttatta
    12661 acagccaatg gtctgccata tctatcgtaa atagttcctc ttggagcaga aataggcaat
    12721 tctcttaaaa tgttggtatt taccttactt tgatgatatt cgccttctat aatctgcagt
    12781 ttaaacaagt gaacacctaa aattgtaaac atcactaaaa aacaaaggga taaaataaac
    12841 aaacgattcg taataaactt tataaatttt tggtaattag aatcaaacat acttaattcc
    12901 tctttctaag caatccattt cttcataata ttatagttca aaaataagta attgaaaata
    12961 taaaaaaagt ctaagaaaaa tataaacatt gtcctaaaaa aacaaactga tacaaataaa
    13021 atatattaaa aaagtgtacg caaaaaacgt acacttattc tctataactc agaacatccg
    13081 tacttccttc taaatggaca tttacattta gctttaaatt ggttatccgg ataggattct
    13141 taatctttat attaggatat tttcttgaaa acatctcttc aatcctgaaa agatcaatgc
    13201 ccttctcttt atacttttca aaagtttcca tacacatttt ttcaaattct ttgcttgcta
    13261 acttttcaat ttttccccgc gcatttgagt cataaaaacg aagatgttcc tgggtttctg
    13321 ccagcgtcac cttaaggtca aaattttttc aagaataatt tcattgtctt cataagaaag
    13381 attggtcgtt aaatttgaat ctaatatttc taatataatc cttgattctt tatgataagg
    13441 atgaggaaca ctgattattt ttacagattc attgccaact aaagcactat gagtagcgga
    13501 atcctttgct tccatcttat caatcatttt tccatctttt ataatccctg aaccttctat
    13561 aacaagatct tcctgaaggg tttcatcttt aagtttaatc attgcaatga ctgaagtaga
    13621 atttccaaca tatctgtcgt ttaaatattc ataaatctgg taaatatccg caaaattttt
    13681 agtaatttca ttgcgtacta aatcataaat ataaagacct acaaattctt cctgacgtac
    13741 ttttaactga atcaaacttc taggatctcc ctcaaatatt aatagatatt gccttaataa
    13801 caattcctgg tcccggttta aatagtcaat aaaattatca ataccgtatt gtgctgctct
    13861 tgtagtaaaa attatgattt tattttgttc ataattgatt ttgtaacttg tacttttatt
    13921 ttggtcccta attgcttcaa acaaattttt ccctttactt gtaaaaataa tccgggttcc
    13981 tgtttcggaa ttgtcttttt tacttctgta agaatgaaat agttcagtat ataataatac
    14041 attttcattc tcgtccacat caataattat tgctgtacaa aaaagagcct tatccatatc
    14101 tctgtaacca aaacaacctg tcagtatgat tagattgatt aaaaaaataa ctaacatttt
    14161 tattttcatt tctggtcatc ctcattaatg gaatctggta aatggtgaat ctctggacgt
    14221 aaaattctgt cactatattg aacagaatct ttggtaccaa ccggatacaa aaagggatag
    14281 ccacaactgg ttagacttgc taaatgggaa atcaataata gtaatccgat taaaaatcct
    14341 ggtaagccta gtaaagatga aaaaataatt aatatatccg tccaaatgga aatagcacca
    14401 aaaatattgg gtgccaaaaa ggctgaaagt gctgttaaag caactaataa aatagcaatg
    14461 gtggatgcca agcccgattg tacggccgtg tctcctaaaa taagagcccc aacaatacta
    14521 atggcttgcg caatcgattg gggtaacctt aaggaagctt ctcttagtat ctgaaagaaa
    14581 aacatcatta ataacacttc cagataaatt ggaaaaggaa cccctgcccg tgca
//
LOCUS       DAOSUD010000054        14383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2529_length_14383_cov_27.731435, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000054 DAOSUD010000000
VERSION     DAOSUD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 14383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14383
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_14383_cov_27.731435"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..2222
                     /gene="uvrC"
                     /locus_tag="PLG49_07660"
     CDS             348..2222
                     /gene="uvrC"
                     /locus_tag="PLG49_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOQ17233.1"
                     /translation="MFDIQEELKKLPEKPGVYLMKDQYENIIYVGKAVNLKNRVRQYF
                     QKSANHSPKVKRMVLQIKEFEYIVTDSEVEALILESNLIKKYQPKYNIRLKDDKNYPY
                     IKITINEDYPRVLMTRKLIKDKAKYFGPYTDGTALKETLDIINKLWPIRTCNRVLPRD
                     IGKERPCLKYHIGQCKAPCAGLISKEEYNKLIEEVIAFLDGKYEEVIKDLEEEMTEAA
                     NELNFEKAAQLRDQINSIKRIAQKQKIINSALEDQDIIAFAKKEDEALVQVFFVRGGK
                     LIGREHFFLDGVDDRTQEEIMTSFVKQFYSGTSFIPKEVILQEEIDELNIIQSWLSSR
                     KGQKVYIKIPRKGEKSKLVEMAAKNALLTLNQFGEEIKRKEKRTKGALEEIQKRLGLE
                     QPIYRIEAYDISNTQGIESVGSMVVFEGGKPKRNDYRKFKIKAVIGPNDYASMEEVIK
                     RRFIHREKELESLKEKGLTLEEGKFTKVPDLILIDGGKGQVNTVIKVLEELNIDIPVC
                     GMVKDDRHRTRGLYYHDEEITFPSSSEAFKLISRIQDEAHRFAIEYHRKLRSKAQIQS
                     VLDEIPGVGPKRKRILLEHFKSVANIKAASLEDIKKLEGIPDWVAENIYDFFHANTRR
                     "
     gene            2313..3260
                     /gene="hprK"
                     /locus_tag="PLG49_07665"
     CDS             2313..3260
                     /gene="hprK"
                     /locus_tag="PLG49_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOQ17234.1"
                     /translation="MYHVKINKIIEKFNLENCTPDIDFEHITISQTDVNRPALQLAGF
                     FDYFDSERVQIIGKVEYTYLSKIDEELRRQTLERLFSFKIPCLIMCRNLRPFPEMITY
                     ANEYQVPILLTEETTSEFMAELIRWLKVELAPRITIHGVLVDIFGEGVLIMGESGVGK
                     SEAALELIKRGHRLVADDVVEIKKVSNQTLIGSCPKVIRHFIELRGIGVVDVKRLYGV
                     GAVKDVHNIDLVIKMELWDDQKEYDRLGLSEEYIEFLGNKVVCNSIPVRPGRNLAIIC
                     ETAAVNHRQKKMGYNAAEELNNRIMENIKRKREGKEFED"
     gene            3283..4227
                     /locus_tag="PLG49_07670"
     CDS             3283..4227
                     /locus_tag="PLG49_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ17235.1"
                     /translation="MYYIGVDLGGTKIAAGLVTEEGKLISKKSIPTLRQRNYQEILKD
                     MANLCLEIIKDNNLSVKDIHSIGVGSPGLPDPENGILVYNNNLNFKNVPIRAELQKYI
                     DLPVYLENDANCAALGEAVAGAAKEFKSSITVTLGTGIGGGIILDGKIYSGSFNAGGE
                     IGHHVIVADGEQCTCGRKGCWEVYASATALIREAKIAAIKHPESQINSLVNGDINRIN
                     AKTPFDAAQNGDKTAKEVIDNYFKYLGMGIVNMVNILQPEAVIIGGGISGQGDYLLNP
                     LRKILDDESYGLESAKTKLKIAQLGNDAGIIGAAMLGK"
     gene            4389..5303
                     /gene="murB"
                     /locus_tag="PLG49_07675"
     CDS             4389..5303
                     /gene="murB"
                     /locus_tag="PLG49_07675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOQ17236.1"
                     /translation="MDRKNLYSKFLEFVPEELIKIDEPMKKHTTFKIGGPADILISPC
                     NKEQIKSILKICKENEVPFYIMGNGSNLLVRDKGFRGVIIQIYKNMSKVKITEDEVWA
                     EAGILLSSLANRIKDESLTGFEFAAGIPGTLGGAVYMNAGAYGGEIKQVLVSAELLDE
                     NGNEIILTNEELELGYRTSLLQRKNYILLSAKLKLHKGNKKEIEEKMNDLAQQRAAKQ
                     PLNMPSAGSTFKRPPGYYAGKLIMDSGLRGYCIGGAEVSQKHCGFIVNKDNATAKDVI
                     DLIEFVQKTVYDKYQVKMEPEVKIIGEE"
     gene            5391..6263
                     /gene="rapZ"
                     /locus_tag="PLG49_07680"
     CDS             5391..6263
                     /gene="rapZ"
                     /locus_tag="PLG49_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOQ17237.1"
                     /translation="MRFVIVTGMSGAGKSTAAKMLEDLGFYCVDNLPPALILKFAEVC
                     SNPGNEIDKVAVGIDTRGGKLFSDLFESLQELKSKGYNYEILFLDASDEVLIKRFKET
                     RRKHPLVWNERISVGIEREREILKEVKEKADYIIDTSNILTRQLKEELYHIFVEEKPF
                     ESIMITILSFGFKYGIPNDSDLVFDVRFIPNPYYIPDMKYKTGNDSEVRDYVISWKES
                     QIFLDKMVDLVEFLIPNYIKEGKNQLVISIGCTGGKHRSVTLTNALYEILKEKGHHTV
                     LHHRDIEKDNLLGR"
     gene            6275..7276
                     /locus_tag="PLG49_07685"
     CDS             6275..7276
                     /locus_tag="PLG49_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOQ17238.1"
                     /translation="MRELNNNPLNHNPKIVAIGGGTGLSTMLRGLKKFTTNITAVVTV
                     ADNGGGSGILREEMKMLPPGDIRNCILALANTEPIMEKLLEYRFEEGRLTGQSFGNLF
                     LAAMTNICGNFEDAVQKMSEVLAVKGKVLPVTKQDVQLCAVFKDGEKICGETQIVEKS
                     KEKRIPIKEVYLSPEKPEPLSGVLEAIEAADVIILGPGSLYTSIFPNLLVNNVPEAIR
                     NSKAVKIYIGNVMTQPGETDGFDLLMHVEELERYVGKGVIDYIIANKDPIPKDLLKKY
                     YEDGASEVNYDARKLAERGFKLIEASVLKIHEDKKLIRHDPEKLATTIMEVITNHCQ"
     gene            7295..8254
                     /gene="whiA"
                     /locus_tag="PLG49_07690"
     CDS             7295..8254
                     /gene="whiA"
                     /locus_tag="PLG49_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOQ17239.1"
                     /translation="MSFSSRVKNELTRHVDAGRHCNIAEISALINICGEVSVHEDKQV
                     SIKFQTENPAVARKYFTLIKKTFNINTEVLVRQNTQLKKNRVYFLYITDSDNSLKVLK
                     ATGLIEEKDGKILRRPGIHPLIIKSTCCKRAYIRGAFLGAGSLSNPEKTYHLEFVNQE
                     YKHSKDLQELINSFNMDAKIIKRKNHYIVYLKEGEQIVDLLNVMEAHLALMDLENVRI
                     IKEMRNNVNRIVNCETANLKKTVSAAIRQVEDIEYIDKTIGLSSLPKNLEEIARYRIK
                     YTEASYKELGAMLDPPVGKSGVNHRLRKLSSIADRLREQKGGL"
     gene            8319..8579
                     /locus_tag="PLG49_07695"
     CDS             8319..8579
                     /locus_tag="PLG49_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ17240.1"
                     /translation="MIEKKVNVTIESGLEARPAALFVQIASQYDSQIHVLLGDKQVNA
                     KSIMGMMSLGVLEGQELTLRAEGSDEEEAVNALVDFLTSHKE"
     gene            complement(8653..9264)
                     /locus_tag="PLG49_07700"
     CDS             complement(8653..9264)
                     /locus_tag="PLG49_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HOQ17241.1"
                     /translation="MDFNKENLIQLINEFSTLEGIALSDIPNIDLYMDQVTTFFDEKL
                     SAWKLNAEDKILTKTMINNYAKADILFPPIKKKYSKEHMLLLILIYHLKQILSLNDIH
                     TVLSPITDKKIQKNFHSIDLFDLYEQFLDIQNKEMKNFEESFSKTVYELFDKTDIGDI
                     GVNDNKNYTLLLIIIINLVISSNIRKVFAEKLIRQFPEIFNQA"
     gene            9486..10139
                     /locus_tag="PLG49_07705"
     CDS             9486..10139
                     /locus_tag="PLG49_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOQ17242.1"
                     /translation="MFKKMREPVNALTHLIGVILSVIGLFFLLYNAAIKGTSRHLISF
                     TIFGISLILLYSASTLYHALNLPKNKIEILQKIDHMMIFVLIAGTYTPVCLTVLEGFW
                     SWSLLICIWIIAFIGILFKIFWFNSPRWLSTLFYIGMGWFVIFFFEPLMNAISLNGLN
                     WLLAGGILYTIGGIIYATKRPRINFKLFGFHEIFHLFVLGGSICHFCFMFHYLLYLH"
     gene            10311..10607
                     /locus_tag="PLG49_07710"
     CDS             10311..10607
                     /locus_tag="PLG49_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17243.1"
                     /translation="MDLEMVNREVADVFSTYETSMENQIRYISGMEHFIQYLYHQNIE
                     LEDVGETEIQQYAESLRALGYPDIFIDTNIESIRKYFSYLQNKNYIKNHTGLID"
     gene            complement(10713..12542)
                     /locus_tag="PLG49_07715"
     CDS             complement(10713..12542)
                     /locus_tag="PLG49_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17244.1"
                     /translation="MTSHDNKKMQPPVAFRSGSHHKIGAPVIKPKNLKATLLRLWDYL
                     KYQKIKLILVIIMVIISSLLMLAGPYLIGVAIDYYIIPRDFPGLLKIVLLLIAAYVLS
                     ALANWLQMYLMVDVSQKTVFKIRKDVFDKLQSLPLKFFDSNTRGELMSRLTNDIDNIS
                     NTLNSSSTQIISSIISITGTVIMMLYLSPILTFINMLIIPVMLFITGKIAKRSRKYFL
                     NQQAALGKLNGMIEESISGQRVIKVFTREEKQIEEFNKSNEELKNIGIKALILSGFIP
                     PLMNLLNNINFAFIAGVGGWLAVREIITIGVIASFINYSKQFTRPINELANQINMFQT
                     AIAGAERVFEIMDESPEKEDEKDAIRLSNISGKVDFENVTFSYDKKTPVLKNINLHIN
                     PGETIALVGPTGAGKTTIINLLTRFYDIDEGLIKIDGTDIRKINRKSLRSSLGIVLQD
                     AYLFSESVRENIRYGRLDASDEEVEEAAKLANAHKFIKRLPQGYDTVLSEEASNLSQG
                     QRQLITIARAILANPSILILDEATSSVDTRTELHIQEAMLNLMKGRTSFVIAHRLSTI
                     RDADQIVVINDGKIIEKGKHDELLKQRGFYYNLYMTQFKNQTI"
     gene            complement(12535..14256)
                     /locus_tag="PLG49_07720"
     CDS             complement(12535..14256)
                     /locus_tag="PLG49_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17245.1"
                     /translation="MTYLKPYRRTAILAPLLMFIEVIMDLLQPTLLSRIVDNGINAGN
                     IDYIIKTGIIMISVAFIGGLGGFGCIISSTITSQNFATDLRSDLFKKIQSFSFSNLDK
                     FKTGSLITRLTNDVTQVQNIVLMSLRIMVRAPLLFIGGIIMAMSINLKLTLILIISIP
                     ILIISVSLIIKKGFPLFTLVQEKIDRVNTVMRENLAGIRVVKAFVREEKEKARFHNAN
                     LELTETMINASKLISSTMPVMMLIMNLSIVAVLWFGGLQINAGAMKEGQLIAFINYLT
                     QILFSLLMIAFILMMVSRAKISAERIIEVLETEVDIKDAETARDIEITRGEVIFENVS
                     FQYTGAKGDAVLKNISFKAESGQTVAILGGTGSGKTTLVSLIPRLYDVSSGRILIDHQ
                     DIRNYKLNALRKGIGVVLQKSVLFSGTIRDNLKWGNKNVSDEKMIAACKAACAHDFIM
                     SFPEQYDTLLGQGGVNLSGGQKQRLSIARALIKEPKILILDDSTSAVDMETESKIQKA
                     LKEMKGTTIFIIAQRISSVMNADKIIVLEDGEIVAEGRHEELLKTSPIYQDIYHSQIR
                     EGESLND"
BASE COUNT         4995 a         2014 c         2757 g         4617 t
ORIGIN      
        1 ttattatcat tatagaagta tagtgatttt ttttcaagtt ataaaaggag aattaaggaa
       61 attgtaatat aaatttaatc aaatgtttaa gatgactttc gaaaatacat agtaattatc
      121 atcagaaagt tttttaatca ctgaaattcc aggattatcc atggcagagg atatgcttta
      181 tgttataatt attaataatg attgttaaat aatattttgc aatagattca cggaatgtga
      241 ttattagtaa gaattcaata atggttttta cattttatat aactgtttat actttatctt
      301 tttattatag agaatgatgg ttatgttttt taagaaagga gtagcctatg tttgatatac
      361 aggaagagct taagaagctg ccggaaaagc ctggagtata tctgatgaag gatcaatatg
      421 aaaatatcat ttatgtgggt aaagcagtca atttaaaaaa cagagtgcgc cagtatttcc
      481 aaaagtctgc caatcattca cctaaggtaa aaagaatggt tcttcaaatt aaagaatttg
      541 aatatattgt aacggattct gaagtcgaag cgcttatttt agaatcgaac ctgataaaaa
      601 aataccagcc caaatacaat atccgtttaa aagacgataa aaattatcct tatatcaaga
      661 ttactattaa tgaggattat cccagagtct taatgacaag aaaactcatt aaggacaagg
      721 ctaaatattt cggtccttat accgatggaa cagcattaaa agagacttta gatatcatca
      781 ataaattatg gcctatcagg acttgtaaca gggttttgcc acgggatata ggcaaggaaa
      841 ggccatgtct gaaatatcat attggtcaat gtaaggcgcc ttgtgccggt cttatttcaa
      901 aagaagaata taataaatta atagaggaag ttattgcctt tttggatggc aaatatgaag
      961 aggtaataaa agatctggaa gaagaaatga ccgaagctgc caatgaatta aattttgaaa
     1021 aagcagccca gttaagagat caaatcaaca gcataaaaag gatagctcaa aagcaaaaga
     1081 taattaattc ggctttagaa gatcaggata ttattgcctt tgccaaaaaa gaagatgaag
     1141 ccttggttca ggttttcttt gtccgcggag gtaagctcat tggaagagaa cattttttct
     1201 tagacggggt tgatgacaga acccaggaag aaattatgac ttcttttgta aaacaatttt
     1261 attcaggtac cagctttatc ccaaaagaag ttatcttgca ggaagaaatt gatgaattaa
     1321 atattattca gtcctggctt tcaagccgta aagggcaaaa agtttacatt aaaattccac
     1381 gtaaagggga aaaatccaag ttagtagaga tggctgcaaa aaatgcttta ctgactttaa
     1441 accaatttgg tgaagaaatt aaacgaaaag aaaaaagaac caaaggtgct ttagaggaaa
     1501 tccagaaacg acttggacta gagcagccga tatacaggat agaagcctat gatatttcaa
     1561 atacacaggg aattgaatcg gtaggttcta tggtagtttt tgaagggggt aaacccaaaa
     1621 gaaatgatta ccgtaaattt aaaatcaagg cggttatagg ccctaatgat tatgcaagca
     1681 tggaagaagt cataaaaagg cgctttattc atcgggaaaa agaacttgaa agtttaaaag
     1741 aaaaaggttt aaccttagaa gaaggaaagt ttacaaaagt tcctgattta attttaatcg
     1801 atggcggaaa aggacaagtt aatactgtaa taaaggtgtt agaagaactt aatatagata
     1861 ttcctgtttg tggcatggta aaagacgaca ggcacagaac taggggtctt tattatcatg
     1921 acgaagagat aacttttcct tcaagttctg aagcttttaa gttaattagc cgtatacagg
     1981 atgaagccca tcgttttgct atagaatatc atagaaaact cagaagtaaa gcccagatac
     2041 aatccgtatt agatgaaata cctggtgtag ggcctaaaag gaaaagaatt cttcttgagc
     2101 attttaaaag tgtagcgaat attaaagcgg cttctctgga agacataaag aagctagaag
     2161 gtatacccga ttgggttgca gaaaatattt atgacttttt tcatgccaac acaagaagat
     2221 gataataaaa cagggaaaat gccattaatt gattgctttt aataaaaact atgataaaat
     2281 aataacaggt tatatagaag agaggggtat ccttgtatca cgtaaaaatt aataagatta
     2341 tagaaaagtt taatttagaa aactgtacgc cggatatcga ttttgagcat attacaatca
     2401 gtcagacaga tgttaaccgt ccggcactcc aattagcagg tttttttgat tattttgatt
     2461 ccgaacgcgt tcagatcatt ggtaaggtag aatataccta tttgagtaag atagatgaag
     2521 aattaagacg ccaaacttta gaacgcttat ttagttttaa aattccatgt ttaattatgt
     2581 gcagaaatct caggcccttt cctgaaatga taacttatgc gaatgagtat caggtgccta
     2641 ttttattgac agaagaaaca acttcagaat ttatggcgga attgatacgt tggttaaaag
     2701 tagagttggc tccaagaata acgattcatg gtgtattggt ggatattttt ggagaaggtg
     2761 ttctcattat gggcgaaagt ggcgttggta aaagtgaagc ggctttggag ttgataaaaa
     2821 gaggacaccg cctagtagcg gatgatgttg tagaaattaa aaaggtttct aatcagactt
     2881 tgataggaag ctgcccgaaa gtaatcaggc attttattga attaagaggt attggagtcg
     2941 ttgatgtaaa gcgtttgtat ggcgttggag ctgtaaagga tgttcataat attgatttag
     3001 tcattaaaat ggaattatgg gatgaccaga aggaatatga ccgtttaggt ctttctgaag
     3061 aatatattga gtttttagga aataaggtgg tttgcaattc tatacccgta agaccaggaa
     3121 gaaatttggc cattatatgt gaaactgcag cagtcaacca cagacaaaag aagatgggtt
     3181 ataatgcggc agaagaactt aataacagaa taatggaaaa cataaaaaga aaaagagaag
     3241 gaaaagaatt tgaggattaa ttcttattta tggagggata atatgtatta tattggcgtg
     3301 gatcttggag gtacaaaaat tgcagcaggg ttagtgacag aagaaggtaa gttaattagt
     3361 aaaaaaagta tacccacttt aagacaaaga aattatcagg aaattttaaa agacatggcc
     3421 aatttgtgtt tggaaattat taaagataat aatctttctg taaaggacat tcattccatt
     3481 ggcgtaggaa gtcctggttt gcctgatcct gaaaatggta ttttagttta taataataat
     3541 ttaaatttta aaaatgtgcc tattcgcgcg gaacttcaaa aatatataga ccttccggtt
     3601 tatttagaaa atgatgctaa ttgtgcggct ttaggagaag ctgttgcagg agcagcaaaa
     3661 gaatttaaaa gttcaattac ggtaacttta ggaacaggaa tcggtggtgg aattatcctc
     3721 gatggaaaga tttacagtgg aagttttaat gcaggtggag aaatcggaca ccatgttatt
     3781 gtggcagatg gagaacaatg tacttgtggc agaaagggtt gttgggaagt ttatgcttca
     3841 gcaactgctt taatcagaga agcaaaaatt gctgcgataa aacatccaga atctcaaatc
     3901 aacagtttag tcaatggaga cattaacaga attaatgcca agacgccttt tgatgcagct
     3961 caaaatggcg ataaaacggc aaaagaagtt atcgataatt atttcaaata tcttggtatg
     4021 ggaatagtta atatggtaaa tattctacag ccggaagcag tgattattgg cggcggcatc
     4081 agtggacaag gagattatct gttgaatcct ttaagaaaaa tacttgatga tgagtcttat
     4141 ggtcttgaat cagcaaaaac gaaattaaag attgcacagt tgggtaatga cgctggtatt
     4201 attggcgcag caatgcttgg aaaataaaag atagacatgg aatatttaac ctctcattat
     4261 ttttgagagg tttttgtttt tatagaaaat atatattata ataaggaaat aaggtaaacg
     4321 attttttgcc ttaaaaattg aatatattta taagggagca taacaatcta tatacagagg
     4381 tgaattgatt ggataggaaa aatttatata gtaaattttt ggaatttgtt cctgaagaat
     4441 taatcaaaat agacgagcct atgaaaaaac atacgacatt taagatagga ggccctgcag
     4501 atatacttat ttctccttgc aataaagagc aaataaagtc tattctcaaa atctgtaagg
     4561 aaaatgaagt tcctttttat attatgggaa acggaagcaa cttattagtc agggataaag
     4621 gctttagagg cgtgattata caaatttata aaaacatgag caaggtaaaa attacagagg
     4681 atgaggtatg ggcagaagcg ggtattttgc tttcctcttt agccaacagg ataaaagatg
     4741 aaagtcttac tgggtttgaa tttgctgcag gaattccggg aactttaggt ggtgctgtat
     4801 atatgaatgc aggggcttat ggcggggaga taaagcaggt attggtcagc gctgagcttt
     4861 tggatgaaaa tggtaatgaa atcattctga cgaatgaaga attagagtta ggatacagga
     4921 cgagcctttt acaaagaaaa aattatattt tactgagtgc aaaattaaaa ttgcataaag
     4981 gcaacaaaaa agaaattgaa gaaaaaatga atgatttagc ccagcaaaga gcagcgaaac
     5041 agccgcttaa tatgcccagt gccggaagta ctttcaaaag accacctggt tattacgctg
     5101 gaaagcttat tatggattca ggtcttaggg gttattgtat cgggggagca gaggtatccc
     5161 aaaaacactg tggctttatc gtgaataagg ataatgcaac ggctaaagac gtgattgatt
     5221 taatagagtt tgtccaaaag acggtatatg ataagtatca ggtaaaaatg gaaccagaag
     5281 ttaaaatcat aggagaagaa taaaatactt tgcaattgtt ttttatccag tgaacttatg
     5341 ataaaataat attatttaat aaaacgcttt taacttaaag gaggaatatc atgcgttttg
     5401 tcatcgtgac aggcatgtcc ggcgcaggta aaagtacagc agcaaagatg ttggaagatt
     5461 taggttttta ttgtgtcgat aatcttcctc ctgctctgat tcttaaattt gctgaagttt
     5521 gttccaatcc gggaaatgaa attgataaag tagctgtggg cattgatacc agaggcggaa
     5581 agcttttttc agatttgttt gagagtttac aagagttgaa atccaagggt tataattatg
     5641 aaatcctttt tttagatgct tcggatgaag ttttaataaa aagatttaaa gaaaccagaa
     5701 gaaaacatcc tttagtttgg aatgaaagaa ttagtgtagg aattgaaaga gaaagagaaa
     5761 tcttaaaaga agtcaaagaa aaagcagatt atattattga caccagtaat attttaacca
     5821 gacaattaaa agaagaatta tatcacatct ttgttgaaga aaaacctttt gaaagtataa
     5881 tgattaccat cttgtctttt ggatttaaat atggcattcc gaatgattca gatttagtat
     5941 ttgacgtgag gtttattcca aatccttatt atattccgga tatgaaatat aagacaggaa
     6001 atgattcaga agttagagat tatgtgataa gttggaaaga aagccagata tttttagata
     6061 agatggttga tttagtagag tttctaattc caaattatat taaagaaggt aagaatcaac
     6121 tggtaatcag cataggatgt actgggggca aacacagatc agttaccctt acgaatgctt
     6181 tatatgaaat tttaaaagaa aaagggcatc atacggtttt gcatcatagg gacattgaaa
     6241 aagataattt attaggaaga tgaggaaaag ttaaatgaga gaattaaata ataatccttt
     6301 aaaccataat cccaaaattg tcgccatagg cggtggaaca ggactttcca ctatgctcag
     6361 gggcttaaaa aaatttacaa cgaacatcac agcagtagtg accgtcgcag acaatggcgg
     6421 tggttcaggc attctcagag aagaaatgaa aatgcttcca ccaggagata tcagaaactg
     6481 tattttagct ttagccaata cagagcctat tatggaaaaa ttactggaat accgttttga
     6541 agaaggcaga ttaacgggtc aaagttttgg aaatttattt ttagcagcta tgacaaatat
     6601 ttgtgggaat tttgaagatg ctgtccaaaa aatgagtgag gtactggcag tgaaggggaa
     6661 ggttcttccc gttaccaagc aggatgtgca actttgtgct gtttttaaag acggggaaaa
     6721 gatatgcggt gaaacacaga ttgttgaaaa gagtaaagaa aaaaggattc caattaaaga
     6781 agtttattta tcgccagaaa agccagagcc tctcagtggg gtattggaag ccatagaagc
     6841 agcagatgtt attattttgg gcccgggaag cttatatact agtattttcc ctaatctttt
     6901 agtaaataat gttccagagg caatccggaa ttcaaaagcc gttaaaatct acataggaaa
     6961 tgtcatgaca caaccaggcg aaaccgatgg ttttgattta ttaatgcatg ttgaagagtt
     7021 agaacgctat gtaggaaaag gtgtcattga ttatattatt gccaataagg atcctatccc
     7081 taaagattta ttaaaaaaat attatgaaga tggggcaagt gaagtaaatt atgatgcaag
     7141 aaaactggct gaaagaggat ttaagttgat agaagcctct gttcttaaaa ttcacgagga
     7201 taaaaagtta atacgccatg atcctgaaaa attagcaaca accatcatgg aggttattac
     7261 aaatcattgc caatagcttg tggaggtgaa aaaattgtct ttttcttccc gggtgaagaa
     7321 tgaattaacc cgacatgttg acgctggaag acactgcaat attgctgaaa tatcggcgtt
     7381 aatcaatata tgtggagaag tatctgttca tgaagacaag caagtttcta ttaaatttca
     7441 aacagaaaat ccagcagttg ctagaaaata ctttacatta ataaaaaaaa cttttaatat
     7501 aaatacagaa gttttagtca gacaaaatac tcaattgaaa aaaaacagag tttattttct
     7561 ttatattacg gactcggata attctcttaa ggtattaaaa gcaacaggtt tgatagagga
     7621 aaaggatggt aaaattttaa gaagaccagg aattcatcct ttaattataa aaagcacatg
     7681 ttgtaagaga gcttatatca gaggagcttt tttgggagca ggttctttaa gtaatcctga
     7741 aaaaacctat cacttggaat ttgttaatca agaatataag catagtaagg atttgcaaga
     7801 attgattaat tcttttaata tggatgcaaa aattattaag agaaaaaatc attatatcgt
     7861 ctatttaaaa gaaggagaac agattgttga tcttcttaat gtaatggaag ctcatttggc
     7921 tttaatggat ttggagaatg tacggattat aaaggaaatg agaaataatg ttaaccgtat
     7981 tgtcaattgt gaaacagcga acttaaaaaa gaccgtatca gcagccataa gacaagttga
     8041 ggatattgaa tatattgata aaacgattgg cttatccagt ttgccaaaga atttggaaga
     8101 gattgcaaga tacaggatta aatatactga agccagttat aaagaacttg gtgccatgtt
     8161 agatcctcct gttgggaaat caggagttaa tcatagactt agaaagctta gcagtatagc
     8221 tgacaggcta agagaacaaa agggaggatt ataaatttaa tcaattgatt tttatttata
     8281 aatatatgtc aagttgacag aacagaatgg aggatattat gattgagaaa aaagtaaatg
     8341 ttacaattga atcaggctta gaagcaaggc cggcagcttt atttgttcag attgccagtc
     8401 aatacgacag tcaaattcat gttttattag gcgataagca agttaatgca aaaagcataa
     8461 tgggtatgat gtctttaggt gttttagaag gacaagaact cacccttcgt gcagagggca
     8521 gcgatgaaga agaagctgta aatgctttag tagattttct tacttcccat aaagaataac
     8581 aaataaaaga ggtctgaaaa aatcaggcct ctttttattt gtttaaatga gcaatatctg
     8641 ttttaggggt gattatgctt gattaaaaat ttccgggaat tgacgaatta atttttcagc
     8701 aaatactttt cggatattag acgaaatcac taaattaatg ataatgataa gaagtaaagt
     8761 gtagttttta ttatcattca cgcctatatc acctatatct gttttatcaa atagttcata
     8821 aaccgtctta gaaaagctct cttcaaagtt tttcatttct ttattttgaa tatccaggaa
     8881 ttgctcatat agatcaaata aatcaattga atgaaagttt ttctgtattt ttttatcagt
     8941 aatgggagat aatacagtgt gtatatcatt tagagaaaga atttgtttta aatgataaat
     9001 caatatcagt aaaagcatat gttcttttga atatttcttt ttgataggag ggaatagtat
     9061 atctgctttg gcataattgt taatcattgt ttttgtaagg attttatctt ctgcatttaa
     9121 tttccatgcg gataattttt catcaaaaaa agtagtgact tgatccatat atagatcaat
     9181 atttggaata tctgaaagag caataccttc taaagtacta aattcattaa tgagttgaat
     9241 aaggttttct ttattaaagt ccataaaatc tctcctaatt tttatctttt acaactatta
     9301 taacatcttt aatcaatatg acaagtagct atcatcaata gataaaaata acttaataat
     9361 atacataatt tagtaataaa atcgacatat taattatatt attaatgtgt tgtgttatat
     9421 aatattattt attaaaatat aaataatatt acaagtagta tttaatacta cacaggagtg
     9481 gttatatgtt taaaaaaatg agagaacctg tcaatgcttt aactcattta ataggtgtta
     9541 tcctatcggt tattggatta ttttttttac tttataatgc tgctataaaa gggacaagca
     9601 ggcatctgat ttcttttact atatttggga taagtttaat tttattgtac agtgccagta
     9661 ctttatatca tgcactgaat ttacctaaaa ataaaataga aattcttcaa aaaatagatc
     9721 atatgatgat atttgtttta attgccggga cttatactcc tgtatgctta acggtgttag
     9781 aaggattttg gagctggagt ttattgattt gtatatggat tattgcgttt atcggcattc
     9841 tattcaaaat tttttggttt aattccccaa gatggttgtc tactttattt tatataggaa
     9901 tgggctggtt tgtcattttt ttctttgaac ctttaatgaa cgctatttca cttaatggtt
     9961 taaattggct acttgcagga ggaattttat ataccattgg aggaattatt tatgccacta
    10021 aaagacccag aataaatttt aagttatttg gttttcatga gatatttcat cttttcgtat
    10081 tagggggcag catttgccat ttttgtttta tgttccatta tctattatat ttgcattaat
    10141 gtttgtattt acccccaatc gtaattacct taaagattgg gggtatttat ctgaaaagtt
    10201 ttctagaatt aaaaacagca aatttatttt tttatatgga aaaatataga actaatgtta
    10261 taataagtat agaatgtttt atcgtttaaa aaattaaaag gaggtcattt atggatttgg
    10321 aaatggttaa cagggaggtt gccgatgtct ttagtactta tgaaacgtct atggagaatc
    10381 agatcagata tatttcaggc atggaacatt ttattcagta tttataccat caaaatatcg
    10441 agttagaaga tgtaggggaa actgaaattc agcaatatgc agaatcttta agagctttag
    10501 gatatccgga tatatttatt gataccaata tagaatccat aagaaaatat ttttcctatt
    10561 tacaaaacaa aaattatata aaaaaccaca ctggtttgat agattaaatt ggggcgtaac
    10621 aataatatat gtgaatacat tttttgcata ataaaaaagt aatgggctgt tacattaaca
    10681 ggtaataact gttaatataa cagccctttg ttttatattg tctggttctt aaattgtgtc
    10741 atatataaat tgtagtaaaa acctctttgt tttaataatt cgtcgtgttt tcctttttct
    10801 ataattttcc cgtcattgat gaccacaatt tggtcagcat ctcttatggt gcttaatctg
    10861 tgggcaataa caaaggaagt tcttcctttc attaaattta gcatggcttc ctgaatatgc
    10921 agttctgtac gagtatcaac actgctggtt gcctcatcaa gaattaaaat tgaaggattt
    10981 gcaagaatgg ctcttgcaat ggtaatcagt tgtctttgcc cctggctaag gttgctggct
    11041 tcttcagaaa gtacggtatc atagccttgg ggaagtcttt taataaattt atgggcattg
    11101 gcaagctttg cggcttcttc tacttcttca tcgcttgcat cgagacgacc gtaacggata
    11161 ttttctctta cactctcaga aaagaggtaa gcatcttgta atacaatacc caaagaagaa
    11221 cgaagacttt tcctgtttat ttttcttata tctgtaccat cgatttttat taaaccttca
    11281 tcgatatcat aaaatcttgt cagaagattg ataatggttg tttttccggc gcctgtagga
    11341 ccaaccaggg caatggtttc accaggattt atatgaagat taatattttt aaggacagga
    11401 gtctttttat catatgaaaa ggtaacattt tcaaaatcaa cttttccaga tatattacta
    11461 agacggatag catccttttc atcttctttt tccgggcttt catccataat ttcaaagact
    11521 ctttcagccc cggcaatagc cgtttgaaac atattgattt gattggctaa ttcattaatg
    11581 ggcctggtaa attgctttga ataattaata aagctggcaa ttacgccaat cgtaatgatt
    11641 tctctaactg caagccagcc gcctacacca gctataaagg caaagttaat attgttaagt
    11701 aaattcataa gaggaggaat aaatcccgat aaaattaaag ctttaattcc tatgtttttt
    11761 aattcttcat tggatttatt aaattcttca atttgctttt cttctctggt aaataccttg
    11821 ataactcttt gaccagagat actttcttca atcattccat ttaattttcc caaagctgcc
    11881 tgctgattaa ggaaatattt tcttgacctt ttcgcaattt ttccagtgat aaagagcatg
    11941 acaggaataa tcagcatatt aataaacgtc agaatcgggc ttaagtaaag catcatgatg
    12001 actgtgccag taatgctgat gatacttgaa ataatctgag ttgaactgct gtttaaggta
    12061 ttactgatat tatcaatatc attggttaaa cggctcatta attcgcctct ggtgttgctg
    12121 tcaaaaaatt ttaaaggcag gctttgcaat ttatcaaaga cgtcttttct aattttgaat
    12181 accgtttttt gtgaaacatc gaccataaga tacatttgaa gccagtttgc cagggcactt
    12241 aaaacgtagg cagcaatcag aagaagaaca attttaagaa gtcctgggaa atcacgggga
    12301 ataatatagt aatctatggc aacaccaatg agatagggac cagccagcat caatagagag
    12361 ctgatgatga ccataataat gactaaaatc aatttaattt tttgatattt caggtaatcc
    12421 caaagcctga gaagtgtagc ttttaagttt tttggcttaa tcacgggagc tccgattttg
    12481 tgatgagaac cgcttctaaa agctacagga ggttgcattt ttttattatc atggctagtc
    12541 attgaggctc tccccctctc ttatttggga atgataaata tcttgataaa tgggacttgt
    12601 tttgagcaat tcttcatgtc tgccttcggc tacaatttct ccatcttcca ggacaataat
    12661 tttatccgca ttcatgacag agctaattct ttgagcaata ataaaaatag tcgttccttt
    12721 catttctttt aaggcttttt ggatttttga ttctgtttcc atatccacag cactggtgct
    12781 gtcatctaat atcagaattt taggttcttt aattaaagcc ctggcaatgg ataggcgttg
    12841 tttttgtcca ccagataaat tgaccccgcc ttgtcctaaa agtgtatcgt attgctcagg
    12901 aaagctcata ataaaatcat gggcgcaagc agctttgcaa gctgctatca ttttttcgtc
    12961 tgaaacattt ttatttcccc atttcagatt atctcttatg gtaccagaaa aaagtacaga
    13021 tttttgaagt acgacaccaa tgccttttct taaggcattt aatttataat ttcggatatc
    13081 ttgatgatca attaaaattc ttccactgct tacatcatac agtcttggga taagactaac
    13141 taaagttgtt ttacctgaac ctgtaccgcc taatatagct actgtttgtc cactttcagc
    13201 tttaaaggag atgtttttta agacagcatc gccctttgcc cctgtatatt ggaaggacac
    13261 attttcaaaa attacttctc cccttgttat ttctatgtct ctggctgttt ctgcatcttt
    13321 aatatccact tctgtttcaa gaacttctat gattctttca gccgatattt ttgctcttga
    13381 gaccatcata aggataaaag caatcataag cagtgaaaat aaaatttgtg ttaagtaatt
    13441 gataaaagca atgagttgtc cttctttcat agctcctgca ttgatttgca agcctccaaa
    13501 ccaaagcacg gctactatgg ataaattcat aataagcatc atgactggca tggtagagga
    13561 gataagtttc gaagcattta tcatggtttc tgtcagctcg aggtttgcat tatgaaatct
    13621 ggctttttct ttttcttctc tgacgaaagc tttcacaacc ctgatacctg caaggttttc
    13681 cctcattacc gtattgacgc ggtcaatttt ttcctgaacc agtgtaaaaa gcggaaagcc
    13741 ttttttaata attaaagata cagaaataat gaggataggt attgaaataa taaggattaa
    13801 ggttaatttc agattaatgg acatggccat aataatcccc ccaataaaca aaagaggggc
    13861 tcttaccatt attcttaaag acattaaaac aatgttttgt acttgagtga cgtcattggt
    13921 gagtctggta atcaatgaac cggttttaaa tttatcaaga ttagaaaatg aaaagctttg
    13981 tatcttttta aataaatctg atctcaggtc cgtagcaaaa ttttggcttg tgatagtaga
    14041 actgataata catccaaatc cccccagtcc cccgataaaa gccacgctga tcatgataat
    14101 tccagtttta atgatataat caatatttcc tgcatttatt ccgttatcta caattctgga
    14161 aagcaaggtg ggttggagta aatccataat aacttctata aacattaata gtggggccag
    14221 gatagcagtt ctacgataag gtttaagata agtcaggatt ttttcatctt attcacctag
    14281 ttaaaattat tagattttgt aacgaacttt atccttatca aatcattttt ataaataatt
    14341 tttacctcaa tatttaaaat atccgatgaa gaatatatta cta
//
LOCUS       DAOSUD010000055        14175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2579_length_14175_cov_25.531232, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000055 DAOSUD010000000
VERSION     DAOSUD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_14175_cov_25.531232"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..771)
                     /gene="ispD"
                     /locus_tag="PLG49_07725"
     CDS             complement(64..771)
                     /gene="ispD"
                     /locus_tag="PLG49_07725"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOQ17246.1"
                     /translation="MDEKLKRHCSVIIPAAGQGKRMGTNIKKQYLKIGDKPIILHTIE
                     KFIKLPSIDEVIVVIAKEDIEYFNNQILKHSDFKHKIKITAGGKERQESVYNGLMTIS
                     DNTEFVLIHDAARPFVSEKDINKLIEATIIYGACVLGVKVKDTIKIADEENYICNTPD
                     RNKLWAIQTPQGFKKDIILEAHKKAKEDNYIGTDDGVLAERLGYRVKIIEGSYENIKI
                     TTAEDLLIGETIIKNLQ"
     gene            complement(838..1905)
                     /locus_tag="PLG49_07730"
     CDS             complement(838..1905)
                     /locus_tag="PLG49_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOQ17247.1"
                     /translation="MKKILRILFSAITGCLIYFLSDFLYTNYLKSLGFLWNVPNNLDN
                     IVMIIIILLVIIVIYITISSFVINLLFKKLKSFEEFFTKMSLKEIMVNILGICVGLVI
                     SNLLGIAILQYGIVGTLIVLALNIIFGYLGYQVVQRKKDEVKIFGTNEYHIAKPKILD
                     TSVIIDGRILDILKTGLVEGKIVIPTFVLEELRHIADSSDALKRNRGRRGLDILNEIQ
                     KQLKVPVEIVEIDYKDIAEVDSKLLKVAQEMDAIIVTNDYNLNKVADFQGVFVFNINE
                     LANAVKPVVLPGEDMEVTVIKDGKEDGQGIGYLNDGTMIVVEGGKDYIGETIKVFVTS
                     VLQTAAGRMIFTRTNGDKASE"
     gene            complement(1972..3003)
                     /gene="tsaD"
                     /locus_tag="PLG49_07735"
     CDS             complement(1972..3003)
                     /gene="tsaD"
                     /locus_tag="PLG49_07735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ17248.1"
                     /translation="MHDNKDILILAIETSCDETAAAVVKNGRVVLSNVVSSQIDLHKK
                     YGGVVPEIASRKHIEKINPVIYEALAQSNMTLEDMDAIAVTYGPGLVGALLVGVATAK
                     AIAFAAKKPLIGVNHIEGHIAANYIENHDFEPPFICLIVSGGHTHLVYVKDYGLYEII
                     GKTRDDAAGEAYDKVARALGIGYPGGPKIDELALEGDPYAIEFPRAYLEDGSFDFSFS
                     GLKSAVLNYLNRSKMMGEAVNINNVAASFQQAVIEVLVTKTIDCAKQKRVNKIALAGG
                     VSANRGLRQLMKKECEKEKIFLQYPSPKLCTDNAAMIGSAAYYDYIRGIRHGYDLNAI
                     ANLRLDDKS"
     gene            complement(3044..3610)
                     /locus_tag="PLG49_07740"
     CDS             complement(3044..3610)
                     /locus_tag="PLG49_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17249.1"
                     /translation="MRKFMAGLLIVIMIAVYFYYMNSKDSNTGQGTVVEKTELSKFEK
                     IKKLNEQQSELFSPNEVIERNNIILSYLYGGELENEEEIDDLILFQRNLFDPDLLAIN
                     PYEEQVEKVKATLEEYKKTGYRIIEIKRDVVQYEKNNPNIAKIRVIQYTNSNKDNYLE
                     YYLRKQEDGSWRILAWEITDEFSILNEF"
     gene            complement(3613..4629)
                     /locus_tag="PLG49_07745"
     CDS             complement(3613..4629)
                     /locus_tag="PLG49_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ17250.1"
                     /translation="MKSRFFIFLLLFFLIFSVVHASETNQDELKKLRGLYFMRQLEQT
                     EKTKGDVVFLFSNVHISSKHFGNLFVYKGKIAIDGEIKGNVYAYGADIYIGEKAKIKG
                     DIRTISSKVSCHQNAEVSGTLGPLFNTLNRFKEQWDEALFFYYTDNIPAYILDLIKMV
                     VQMVISLLCLSLFKRSVILQGKILTCQTKDVFNRGLILYLMFTALVLVFTLSIIAFPL
                     VLVLIAACYLLTQYGKAALALLIGEWLINKIAVKTDIYIKFWTGILVIQLMNFAPLMD
                     WCGNIVLLPLISFGMLAQMIINQLTNSSYVVVNTEYEGELNFYYQAQLYEQITKNLRG
                     KKGG"
     gene            complement(4629..5195)
                     /locus_tag="PLG49_07750"
     CDS             complement(4629..5195)
                     /locus_tag="PLG49_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOQ17251.1"
                     /translation="MKCSKADQLMMKYMDGILSMEEAKQLNLHLTECDACKEEFFAYQ
                     KMIDVLQDGQFYKAPDDFESTVMNRIKDIEVEYEIKKTVPIETISAIVWGFFSLIFGI
                     GVLLVLYRQPVLEYLVQNPYLGEWANSMIPAMDILSAYINDFKTEMTNILGTSKYIFE
                     VLRLILIPIVAVLGVVKYYIYRKEKVEI"
     gene            complement(5185..5745)
                     /locus_tag="PLG49_07755"
     CDS             complement(5185..5745)
                     /locus_tag="PLG49_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ17252.1"
                     /translation="MIRLEDYEVVQACLQGKKEMFEILVSRYKNLVYSIILRMTNDAE
                     EANDLAQEVFIKVYRNLDKYQPDYKFSTWIMKITTNLVIDARRKSKPNTISVDEMLIE
                     PSTEKGPEQGYLEQEKINEVIAALNALPDMYKVPMILFHQQGLSYQEIAEIINEPLSK
                     VKNRIFRARKMLKESLLSKEGGVYEM"
     gene            complement(5859..6785)
                     /locus_tag="PLG49_07760"
     CDS             complement(5859..6785)
                     /locus_tag="PLG49_07760"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOQ17253.1"
                     /translation="MLDICLLGTGGMMPLPNRWLTALLVRYQGRKILIDCGEGTQITL
                     KTLGWGFKTIDVICFTHYHGDHVTGLPGLLLTIGNAGRTEPLTLIGPPGLKKVVEGLC
                     VVTPELPFEIKLIEVNEEEAKEMRIEIDRGLFIKMLPVFHRITCFAYVLELERRPKFI
                     PEKARTLNIPVEYWKHLQQGETVRINDKIYSPEMVSGEKRKGLKIAYCTDSRPVENLV
                     DFIADADLFICEGMYGDDADKEQAKKKKHMVFSEAAQLARQAGVKELWLTHFSPALTE
                     PQNFIKVATDIFPYTKVGEDRMTKNLNFLEEK"
     gene            6956..7894
                     /locus_tag="PLG49_07765"
     CDS             6956..7894
                     /locus_tag="PLG49_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOQ17254.1"
                     /translation="MKKIMVSIILLSIISLTLYGCFPEKSNSEAEKNTLTVYASIYPL
                     YDFTKKIGKEKVNVHLIIPAGAEPHDWEPSAKLMAKIQEADLFIYNGLGMESWAERII
                     QAVDHPDLIVVNTSDNIEWLELGEEDDHHHDKESKHSYDPHLWLDPLRAMQQAEHIKN
                     ALIEADSKNKDFYEDNFLKLKEEFLKLDKEYQIALSQLSKNEIVVSHAAFGYLADRYN
                     LRQLSVSGLSPQAEPSPNEMANLTDFIKEHNVEYIFYEPLSNSKLIEVIAEETGVKML
                     TLNPLGNITQEDLDEGKDYFSIMRENLESLKKALTE"
     gene            7913..8662
                     /locus_tag="PLG49_07770"
     CDS             7913..8662
                     /locus_tag="PLG49_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOQ17255.1"
                     /translation="MNKILEVSHLTFAYEKNLILDDISFEINEGDFAGIVGPNGSGKS
                     TLIKIILGLLKPIKGSIKLFEKDIRHFDDWAKIGYISQKAASFNSSFPATVEEIVAAN
                     LYPKIGLFKRIKKEHMEKVYEMLKIVGMEDYRKQLIGNLSGGQQQRVFIARTLVSSPK
                     ILFLDEPTVGIDMKSQENFYELIYKLNKTMNITVIMISHDIGVMTQKANKVLCMGNKK
                     LIVHDSSSDTKITETLAKIYGDKMHLLLHHH"
     gene            8711..9535
                     /locus_tag="PLG49_07775"
     CDS             8711..9535
                     /locus_tag="PLG49_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOQ17256.1"
                     /translation="MPEILQYAFMQRALLAGIIIALLCPLIGIFIVLRRMSLIGDALS
                     HVALSGVVAGILLKISPLIVTLIFSVTAALGIEKLRKRYEKYAELAISIIMSAGMAVA
                     VILISLTKSINIDLFGYLFGNITTVLSQDLWLILLLGIVVMISVFLLYKELFYMSFDE
                     EAAALAGIPTRAINVYFVILVAITITLSMRIVGILLISSLMILPVAASLQIAKSFKSV
                     TILSIVFAQISVILGIIISYYFELASGGTIVLISVVILLTVLAVKNIINIRKINFI"
     gene            complement(9580..10944)
                     /locus_tag="PLG49_07780"
     CDS             complement(9580..10944)
                     /locus_tag="PLG49_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOQ17257.1"
                     /translation="MSKKTVKKVILPENEIPKQWYNVLADMPNKPEPYFSSVTNKMAT
                     PDELEVIFPKELIQQEMSQERWIDIPDEVREMYKQWRPSPLYRAVGLEKLLDTPARIY
                     YKYEGVNATGSHKLNSAIPQAYYNKMAGIKKLSTETGAGQWGSALSLACNYFGLECEV
                     YMVKVSYEQKPYRRLFMQTFGANVIASPSNLTQSGRAILEKDPDSTGSLGIAISEAVE
                     VAATNPDTNYALGSVLNHVCLHQTVIGLEAKKQLELLDEYPDIVIACCGGGSNFAGLS
                     FPFVKDKLNGTNIRLIAVEPTACPSLTKGVFAYDYGDTAKVGPIAKMYTLGHDFVPAG
                     IHAGGLRYHGASPIVSQLYHDGIIEAQAVPQRDVFDAAVKFAKTEGIIPAPESAHAIR
                     AAINEAIKCKETGEAKVILFNLSGHGYFDMAAYDNYFSGKLIDVDYPEDLIKENIRSL
                     PDIK"
     gene            complement(11178..11615)
                     /locus_tag="PLG49_07785"
     CDS             complement(11178..11615)
                     /locus_tag="PLG49_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOQ17258.1"
                     /translation="MNMIRVEEILKKAGLKLTTQRRAIIEVFMDYNDHVLTAEQIYEK
                     TKEKHPTMNFSTVYRNLEILERINIIHKITMQNQASKYQLLHYGKHHHHFICKDCGKS
                     EIIDFCPLRTLNTELVNKNFVLTDHCFELYGYCNDCMANKSEA"
     gene            complement(11655..11882)
                     /locus_tag="PLG49_07790"
     CDS             complement(11655..11882)
                     /locus_tag="PLG49_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17259.1"
                     /translation="MRKYKAVPIEKEVIDEVYCNVCGKKIEKVSPDQFEDYLHIEKLW
                     GYHSKNDGKKDEIDVCQACYEKWINSFKISV"
     gene            complement(12072..13061)
                     /locus_tag="PLG49_07795"
     CDS             complement(12072..13061)
                     /locus_tag="PLG49_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ17260.1"
                     /translation="MIRLKDIADALNVSITTVSKALNGHPDISESRRKEILEYAEKLN
                     YVPNQVARSFRQQRTKIVGIILSDNSNPYNARIIRGIEETFSSKGYYTIIMNNHEDPE
                     KELKLVSELCGLNVAGVLLTPAAGNKKSRELLRQFNIPYVLVNRYINEKEDTYVVVDD
                     WKAAYIGTKHLCSFCYDKIFFLNFLSSVSSSKNRLKGYKAALEEYKIEMDESWVIPDC
                     KNQQDGYKAMLKILEKNKPPFSVLCYSDYIAIGAICALQERLIRIPEDVAVMGTDDID
                     ILSFVKPRLTTVGVPKLRLGIRCAELLIDLIRKKETEEKRIVMKPELIIRETS"
     gene            complement(13193..>14175)
                     /locus_tag="PLG49_07800"
     CDS             complement(13193..>14175)
                     /locus_tag="PLG49_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOQ17261.1"
                     /translation="KNLFLSLIYYLGGTTVGINVDIVVLGNIINEMIIFPNKIIGPVL
                     GSPCAYSSIIMARLGRKVGIVSYIGDDLDKDMVEQFQIVDTEGFIPYKFTTHNNLIFH
                     ADGKKSVEYAKVAPVIEFEDIPENYLKAETFFICPMNCEVSIEIAKKLHEMGKRVIVD
                     LGGFGGTTSYNHFSIDTRRGKALIEYLCMYSTIIKASEEDLKYIGPDIPLGTMSQYFI
                     DKGTQSVVITMGEKGALIQSKGQAPKHIDVFPPECPEEEYNLVGAGDALAAGMIASLD
                     DMDSLEEAVIFGTAVASLVIEKKGGCVVKRMPTSFEVAKRLSKRSSVMHG"
BASE COUNT         4516 a         2771 c         1905 g         4983 t
ORIGIN      
        1 catcttaaat ccaatgtcaa gatataatat attaatttct cacatgaaat ttttatcaaa
       61 tttttattgt aaatttttaa tgatagtttc gccaataagt aaatcttctg ctgttgtaat
      121 tttaatattt tcatagcttc cttcaataat ttttacacga tagcctaacc tttctgctaa
      181 tacaccatca tctgttccta tataattatc ttcttttgct tttttatgtg cttctaatat
      241 aatgtctttt ttaaatccct gaggtgtttg gattgcccat aatttattcc tgtcaggtgt
      301 attacaaata tagttttctt catctgctat tttaatggta tcttttactt tcacacctaa
      361 aacacaggcg ccataaatta tagtagcttc tataagctta ttaatatctt tttctgaaac
      421 aaaaggtctg gcagcatcgt gtattaaaac aaactccgta ttatcagata ttgtcatcaa
      481 gccattatac actgattctt gcctttcttt tccgcctgct gttattttta ttttatgctt
      541 aaagtcagag tgctttaaaa tttgattatt aaagtattct atatcttctt ttgctataac
      601 tacaatgact tcatcaatgg atggcagctt aataaatttt tcaattgtat gaaggataat
      661 cggcttatcc cctatcttca gatattgttt ttttatgttc gtgcccatcc tttttccctg
      721 tcctgctgca ggaatgataa ccgaacaatg tctttttaat ttctcatcca aaatatcacc
      781 tcaagtcctg ctgaatatat aatcaactat cctgttcaaa agaaagctcc ggagtcatta
      841 ctccgaagct ttgtctccat tcgttcttgt aaatatcatt ctaccggctg ccgtttgcaa
      901 tacactggtg acaaatactt ttatggtttc gccaatataa tccttgccac cttcaacgac
      961 gatcatcgta ccgtcattta aataacctat gccttgacca tcttctttac catcttttat
     1021 tacagttact tccatgtctt caccaggaag aactacaggt ttcactgcat tagcaagttc
     1081 gttaatgtta aagacaaaga caccttgaaa atcagctact ttatttaaat tataatcatt
     1141 cgttacaata atggcatcca tctcttgagc aacttttaat aacttgctgt ctacttcagc
     1201 gatatcttta tagtctatct ccacaatttc taccggaacc ttaagctgtt tttgaatttc
     1261 atttaatatg tcaaggcctc tccttcccct gtttcttttt aaagcatcag aggaatctgc
     1321 aatgtgcctt aattcttcta aaacaaaagt tggaattact attttacctt ctactaaacc
     1381 cgtttttaaa atatctaaaa tccttccatc aataatgaca ctggtatcta aaatcttagg
     1441 cttagcaata tgatactcat tggtaccaaa gattttaact tcatctttct tcctttgaac
     1501 cacttgatat cctaaataac cgaaaattat attgagtgct aatacaatta aggttcctac
     1561 tattccgtat tgaaggattg cgattcctaa taaattcgat ataactaatc ctacacatat
     1621 ccctagaata ttaaccataa tttctttcaa agacatttta gtgaagaact cttcgaaaga
     1681 ttttaacttt ttaaataata aattaataac aaaagaagat attgttatat aaataactat
     1741 aatgaccaat aaaataataa taatcatcac tatattatcc aaattattag gcacattcca
     1801 aagaaagccc aaagatttta gataattagt ataaagaaaa tcagataaaa aataaatcag
     1861 acagccagtg attgccgaaa aaagaatcct taagattttc ttcatattgt atattcaccc
     1921 cctctcaatt attgccaaat aattcagtaa tatacaataa attataaata tttatgattt
     1981 atcatccagt cttaaattag ctatggcatt taaatcatag ccatgcctga ttcctcttat
     2041 ataatcatag taagctgcag acccaatcat cgcagcatta tctgtacaca atttagggga
     2101 tggatattga agaaaaatct tttctttttc acattctttt ttcattaatt gtctaagccc
     2161 cctgtttgca gatactccac cagcaagtgc aattttatta actctctttt gttttgcaca
     2221 atcaatggtt tttgtcacta atacctcaat caccgcttgt tgaaaactag ctgcaacatt
     2281 attgatatta actgcttctc ccatcatttt gctgcgattt aagtaattaa gtactgcaga
     2341 tttcaaacca ctaaagctga aatcaaaaga accatcttct aagtatgctc tgggaaattc
     2401 aatggcataa ggatccccct ctaaagctaa ttcatcaatt ttaggccccc ccggataacc
     2461 aattccaagg gctcttgcaa ctttatcata agcttcccct gcagcatcgt ctcttgtttt
     2521 tcctattatt tcgtataaac cataatcttt aacgtaaacc aaatgcgtat gacctcctga
     2581 tacaattaaa cagataaaag gaggttcgaa atcatgattt tctatataat tggctgcaat
     2641 atgaccttct atatgattaa caccgattaa cggtttcttt gcagcaaaag ctattgcttt
     2701 agcagtagcc actccaacaa gcaaagctcc tactaagccc gggccataag tcacagcaat
     2761 ggcatccata tcttccaggg tcatattgga ttgagcaagg gcttcataaa taactggatt
     2821 aattttttct atatgctttc tggaagcaat ctcaggaaca actccgccat attttttatg
     2881 aagatcaatt tgcgaagaaa caacattgga taataccact cttccattct ttacaactgc
     2941 agccgctgtt tcatcgcagg acgtctctat agccagtatt aatatatctt tattatcgtg
     3001 catataaatg tcctttctaa ataagtcaaa acttttttat ccattaaaat tcattcagaa
     3061 ttgaaaattc atccgtaatt tcccatgcta aaattctcca actgccatct tcttgctttc
     3121 ttaaataata ctctaaatag ttatccttat tactatttgt atactgaatg actcttattt
     3181 ttgctatatt aggattattc ttttcatatt gtaccacatc tcgtttaatt tcaataattc
     3241 tataacccgt ttttttgtat tcttcaaggg ttgcttttac tttttctact tgttcttcat
     3301 aaggattgat tgctaataaa tcaggatcaa ataaatttct ctgaaagaga attaagtcat
     3361 caatttcttc ctcattttct aattctccac catacaagta tgacaaaata atattatttc
     3421 tttcaatcac ttcattagga gaaaataatt cactttgctg ttcattaagc tttttaattt
     3481 tttcaaactt tgataattcg gttttttcaa caactgttcc ttgcccagta ttactatctt
     3541 tactattcat ataataaaaa tatacagcta tcataataac gatgagtaat cctgccatga
     3601 attttctcat gattatcctc cctttttccc ccgtaaattc tttgttatct gttcatacaa
     3661 ttgggcttga taataaaaat ttaattcacc ttcatattct gtatttacga caacataaga
     3721 agaattcgtt aattgattaa ttatcatttg agcaagcatc ccaaagctga taaggggtaa
     3781 tagcacaata ttcccgcacc aatccatgag tggagcaaaa ttcattaact gaatgaccaa
     3841 tattcctgtc caaaatttta tataaatatc ggttttgact gctattttat tgataagcca
     3901 ttcccctata agaagggcta aagctgcttt cccatactgt gtcagcaaat aacaagccgc
     3961 tattaaaaca agaactaaag gaaaagcaat gatcgataaa gtaaatacca aaacaagggc
     4021 tgtaaacatt aaatataata ttaagcccct attaaaaaca tcttttgtct gacaggttaa
     4081 aattttgcct tgaagtataa cacttctttt aaaaagggat aagcataata agcttatcac
     4141 catctgtaca accattttta ttaaatccaa tatatatgca ggaatattat ctgtataata
     4201 aaaaaataaa gcttcatccc actgttcctt aaaacggttt aatgtattaa aaagaggtcc
     4261 aagggtacca gatacctctg cattctgatg gcatgatact tttgatgata ttgttcttat
     4321 atctcctttt atttttgctt tttcaccaat atatatatcg gcaccataag cataaacatt
     4381 accttttatt tcgccatcaa ttgcaatttt tcctttataa acaaataaat ttccaaaatg
     4441 ctttgaagaa atatgtacat tagaaaacaa aaagacaaca tctccttttg ttttctctgt
     4501 ttgctcgagt tgccgcataa aatataagcc tcttaacttt tttaattcat cttgattcgt
     4561 ctccgaagca tgtacaaccg aaaagattaa aaaaaataaa agtaaaaaaa taaaaaagcg
     4621 ggatttcatt atatttctac cttttccttg cgataaatat agtatttaac aactccgaga
     4681 acggctacaa taggaatgag tatcaatctt aaaacttcaa aaatatactt actggtgcct
     4741 aaaatattag tcatttctgt tttaaaatca ttaatataag cagaaagaat atccattgca
     4801 ggtatcatag aattcgccca ttcgcccaaa taaggatttt gaacaagata ttccagaaca
     4861 ggctgcctgt acaatactaa taacacacct attccaaata ttaaagagaa aaatccccat
     4921 actatcgcag aaatggtttc tatgggtacc gttttcttaa tttcatactc tacttctata
     4981 tcttttatac gattcattac tgttgattca aaatcatccg gggctttgta aaactgacca
     5041 tcctgaagta catcaatcat tttttgataa gcaaaaaatt cttctttaca agcatcacat
     5101 tcagtaagat gaaggtttaa ttgtttagct tcttccatac taagaatccc gtccatgtac
     5161 ttcatcatca actggtctgc tttgctacat ttcataaact ccgccctcct ttgacaacaa
     5221 actttctttt aacatttttc ttgctctgaa tatacgattt tttacctttg ataaaggttc
     5281 attaataatt tctgcaattt cttgatagga taaaccttgc tgatgaaata agatcatagg
     5341 aactttatac atatcaggta aagcatttaa cgcagctata acttcattta tcttttcttg
     5401 ttccaaataa ccttgctcag gtcctttttc cgttgaaggt tctattaaca tttcatccac
     5461 agaaatcgta ttaggcttgc tttttctgcg tgcatcgatc accaaatttg tagtaatttt
     5521 cataatccag gttgaaaatt tgtaatctgg ttgatattta tcaagattgc gatacacttt
     5581 tataaaaact tcctgtgcta agtcattggc ttcctcagca tcatttgtca tcctcaatat
     5641 aatagaatat actaagttct tataacggga aaccaatatt tcaaacattt cctttttccc
     5701 ctgaagacaa gcttgaacta cctcataatc ttccaacctt atcaccgcca attatctatg
     5761 cagtctatca ttttaaatga tatttcttca atctatatac gaattggcct atacaaagtc
     5821 tgaaatgaat aattaagctt attataccat aattaaaatt atttttcttc aagaaagttt
     5881 aaattttttg tcatcctatc ttcaccaact tttgtatagg gaaaaatatc tgtagccact
     5941 tttataaaat tttgcggttc tgttaaggcc ggactaaaat gagtaagcca cagttctttt
     6001 acacctgcct gccttgccag ttgagctgcc tctgaaaaaa ccatatgctt tttctttttt
     6061 gcttgttctt tgtcggcgtc atccccatac atgccttcac atataaataa atctgcatcc
     6121 gcaataaaat caaccaggtt ctcaactggc ctgctgtctg tgcaataggc aatcttaagc
     6181 ccttttcttt tttcacctga taccatttca ggactataaa ttttatcatt gatccgaaca
     6241 gtttctccct gttgaagatg tttccaatat tcaacaggaa tatttaaagt tctggctttt
     6301 tcaggtataa atttgggtct tctctccagt tccaatacat aagcaaagca tgtaattctg
     6361 tgaaaaaccg gcagcatctt aataaaaaga cctctatcaa tttcaattct catctctttg
     6421 gcctcttctt cattcacttc tatcaactta atttcaaaag gtaattctgg cgtaacaaca
     6481 cacaaacctt caacaacttt ttttaaacca ggagggccaa taagggtaag aggctcagtc
     6541 ctgccagcat tcccaatggt taatagaaga ccgggaaggc ctgttacatg gtctccatga
     6601 taatgggtaa aacaaatcac atcaatggtt ttaaaacccc agcctaatgt ctttaaagta
     6661 atttgtgttc cttcaccaca atctatcaat atctttcttc cctgatatct aaccagtaaa
     6721 gcagttaacc atctattggg taaaggcatc atacctccgg tgcctaaaag acaaatatct
     6781 aacatagtaa cctccttatg ggaaacaaaa gcttttaata tattatatca ataatccggt
     6841 tctaataaag gcataatata aattacatct tgcaatttct aaatgggtgt gttattagta
     6901 aaatagctta aatgcaaatc ttttgcattt agaaatatat aaaggagacc aaagcatgaa
     6961 aaaaataatg gtatctatta tcttattatc tattatatca ttaactctat acggctgttt
     7021 ccccgaaaaa agcaattcag aagctgaaaa aaacactctt actgtatatg caagtattta
     7081 tcctttatat gattttacta aaaaaatcgg aaaagaaaaa gtaaatgtcc atctcattat
     7141 accagcagga gcagaacccc atgactggga accttctgca aagctcatgg caaaaattca
     7201 agaggcagac ctttttattt ataatggatt aggcatggaa tcatgggctg aaagaatcat
     7261 tcaggcagtg gatcatcctg acctgatcgt tgttaacact tctgataata ttgaatggct
     7321 ggaactggga gaagaggatg accatcatca tgataaggaa agcaagcaca gctacgaccc
     7381 gcatctttgg ctggatcctt taagagcaat gcagcaagct gaacacataa aaaatgcctt
     7441 aattgaagca gacagcaaga ataaagactt ttatgaagat aatttcttaa aacttaaaga
     7501 agaattcctg aaattagata aagaatacca aatagcttta tcacaacttt caaaaaatga
     7561 aatcgttgtt tcccatgcag cctttggata cctggcagac agatataatc ttagacagct
     7621 ttccgtttca ggcctgtctc cccaggccga acccagccct aatgaaatgg ccaatttaac
     7681 tgattttatc aaagaacata atgtggaata tattttttat gaacctttat ccaattcaaa
     7741 attaattgaa gtcattgctg aagaaaccgg tgttaaaatg ctcaccctga atcctttagg
     7801 gaatataaca caagaagacc tggacgaagg aaaagactat ttttccatca tgagagaaaa
     7861 tcttgaatct cttaaaaaag ctttaactga atgaaaagga aagaggaaaa gtatgaataa
     7921 aatattagaa gtcagccact tgactttcgc ttatgaaaaa aacctgatac tagatgatat
     7981 ttcctttgaa atcaatgaag gagactttgc tggtattgtt ggtcctaatg gttctggaaa
     8041 aagtacctta ataaaaatca tacttggctt attaaagcca attaaaggca gcattaagct
     8101 tttcgaaaaa gacatccgtc attttgacga ttgggcaaaa ataggttaca tctctcaaaa
     8161 agctgcttca tttaacagca gctttcctgc aactgtagaa gaaattgttg ctgcaaactt
     8221 ataccccaaa attgggcttt ttaaaagaat taaaaaagaa catatggaaa aagtttatga
     8281 gatgcttaaa attgttggaa tggaagacta ccgaaagcaa ctaattggca atctttccgg
     8341 cggacagcag caaagagttt ttattgccag aactctggtc agctctccca aaatcctctt
     8401 tctggacgag cctactgttg gcatagatat gaagtcccag gaaaattttt atgaattaat
     8461 ctataaactt aataaaacca tgaatattac cgtcataatg atttctcatg acataggggt
     8521 catgactcag aaagctaata aagtattatg tatgggaaat aaaaagctaa ttgtgcacga
     8581 tagttcttct gatactaaaa ttactgaaac acttgccaaa atatacggag ataaaatgca
     8641 tctgctcctg catcatcact aagattttct taacttattc tttataaatc cactaaaagg
     8701 agtcattgat atgccagaga tactacaata tgcctttatg caaagagctc ttcttgcagg
     8761 cattattatt gctttattat gtcctttaat cgggatattt attgtcttaa ggcggatgtc
     8821 attaatagga gacgctttat cccatgtcgc cctttctggc gttgtagctg gaatactttt
     8881 aaagatttct cctttaattg tgacattgat tttttctgta acagcagcgc tcggaataga
     8941 aaaactcaga aaaagatatg aaaaatatgc agaactggcg atatccatca ttatgtctgc
     9001 cggaatggca gttgccgtta ttttaatcag cctgaccaaa tcaatcaata ttgatttatt
     9061 tggttatctc tttggaaata ttacaacggt tctttctcag gatttatggc taattttgtt
     9121 gctgggtatt gtcgttatga tttccgtttt tcttctttat aaagaacttt tttatatgtc
     9181 ttttgacgaa gaagcagccg ctcttgccgg aattcccaca agagcaatta atgtttattt
     9241 tgtcattctg gttgctatta ccataacgct ttctatgcgc attgtcggaa tcttattaat
     9301 atcttcttta atgatcctgc ctgttgcggc aagccttcaa attgccaaaa gttttaaatc
     9361 cgttaccatt ctctccatag tatttgcaca aatttcggtt attctgggta ttattatttc
     9421 ctattatttt gaattggctt ccggaggaac cattgtcctt atttcagttg ttattctttt
     9481 gaccgtcctg gcagtaaaaa acattatcaa cattagaaaa ataaacttta tttaaatgaa
     9541 aagacccgtc tttacggacg ggtctttttt aaacaagaat tatttaatat ctggtaaact
     9601 tcttatattt tcttttatta aatcctctgg ataatccaca tcgatcagtt ttccgctaaa
     9661 ataattgtca taagcagcca tatcgaaata gccatgtcca cttaaattaa ataaaatcac
     9721 ttttgcctct cctgtttctt tacactttat agcttcatta attgctgctc tgattgcatg
     9781 cgcagactct ggtgctggta taataccttc tgtctttgca aattttactg ctgcatcaaa
     9841 gacatccctt tgaggaaccg cctgagcttc aataattcca tcgtgataaa gctggcttac
     9901 tattggggac gccccatgat atcttaaacc cccggcatga atgcctgcag gaacaaaatc
     9961 atgacccaaa gtatacattt tagcaattgg tccaaccttt gctgtatcac cataatcata
    10021 agcaaaaact ccttttgtca gtgaaggaca agctgtaggt tctacagcaa ttaatcggat
    10081 attcgttcca tttaatttat cttttacaaa ggggaagcta aggcctgcaa aattacttcc
    10141 tccaccgcag caagcaatta caatatcggg atattcatct aaaagttcaa gttgtttttt
    10201 tgcctcaaga ccaataacag tctggtgcag gcacacatga tttaaaacac tgcctaaagc
    10261 ataattggta tccggattgg ttgctgcaac ttcaaccgct tcactgatag ctattcccag
    10321 gctgcccgta gaatccggat ctttttctaa aattgccctg cctgactgcg ttaaattact
    10381 tggacttgca ataacattgg ctccaaaagt ttgcataaat aaacgcctgt aaggtttttg
    10441 ttcataactg actttaacca tatatacttc gcactctaag ccaaagtaat tacatgcaag
    10501 gcttaaagca cttccccatt gaccggcacc ggtttctgtt gaaagctttt taataccagc
    10561 cattttattg taataagctt gtggaatagc agaatttaat ttatgacttc ctgtcgcatt
    10621 gacaccttca tatttataat atattctagc tggtgtatcc aaaagttttt ccaggccaac
    10681 agccctatag agaggacttg gtctccactg tttatacatt tctctaactt catccggaat
    10741 atcaatccat ctttcctgag acatttcttg ctgaatcaat tctttaggga aaattacttc
    10801 aagttcatca ggtgttgcca tcttatttgt cacggaacta aaatagggtt ctggcttatt
    10861 aggcatatct gccagcacat tataccattg ctttggaatt tcattttcag gtaaaatcac
    10921 ttttttaacg gtttttttgc tcatcttcct tatctctcct cgccaaaaac ttagtttata
    10981 ttttttataa aaagctcatg gttctgtcag cagatattat gtaaaaaaat atttaaattt
    11041 cgtataattc taccatactc ttgtcttttt tacaataaat aatattacta ttttcccata
    11101 ttcatagtct tttaagttcc tttatatgaa ttgaatctct cttaaaatct aacttttttc
    11161 atataaaaaa gtgaagctta agcttcactt ttatttgcca tacaatcatt acaatatcca
    11221 tataactcaa aacaatgatc cgttaaaaca aaatttttgt tcaccaattc tgtattaaga
    11281 gttcttagag ggcaaaaatc aataatctcc gattttccac aatctttaca aataaaatga
    11341 tggtgatgtt taccataatg aagcaattga tattttgagg cttgattttg catagtaatt
    11401 ttatgaataa tattaattct ttctaatatt tctaaatttc tataaactgt agaaaaattc
    11461 attgtcgggt gcttttcttt cgttttttcg taaatttgct cagcagtcaa aacatggtca
    11521 ttataatcca taaatacttc tatgatggct cttctttggg tagttaattt taaacccgcc
    11581 ttttttaaaa tctcttcaac cctaatcata ttcattatgg gcactcctaa tacaatatgg
    11641 tactgatgta atggctaaac agaaatttta aagctattta tccatttctc ataacaagcc
    11701 tgacatacat ctatttcatc ttttttacca tcatttttag aatgataacc ccaaagcttt
    11761 tctatatgca aatagtcttc aaactgatcg ggagaaactt tttcgatttt tttcccacat
    11821 acattacaat aaacctcgtc tataacttct ttttcgattg gtactgcttt atattttctc
    11881 atattaagcc tccattatca tttcatgatg agaaaaatca caaattgtca aattacatta
    11941 agtatattat acctttctat ttttcccatg tatagaggta atatttgatt tgatttgagc
    12001 tttttgtaaa ttgattactt ttatttacta tattggtcaa tattacaggg ttttctttat
    12061 aatttgtctt attaagaagt ttctctgata attaactcag gcttcatgac aattcttttt
    12121 tcttcagttt ctttttttct aattaaatca atcaataatt ctgcacatct tataccaagt
    12181 cttaattttg gaacaccaac cgttgtcagt cttggcttaa caaaggataa aatatcaatg
    12241 tcatctgttc ccattactgc aacatcttcc ggtattctga ttagtctttc ctgtaaagcg
    12301 caaattgctc caatggcaat ataatcactg taacacaata cagaaaaagg aggtttattt
    12361 ttttctaata tctttaacat tgctttataa ccatcttgtt gatttttaca atctggtatt
    12421 acccagctct catccatttc tatcttgtat tcttctagcg ctgctttata acctttaaga
    12481 cggttttttg atgacgatac acttgataag aaatttagaa aaaatatttt atcataacaa
    12541 aatgaacata gatgtttcgt tccaatataa gctgctttcc aatcgtcaac cactacatat
    12601 gtatcttctt tttcattaat gtaacggtta accaatacat aaggtatatt aaactgtctt
    12661 aataattccc tgcttttttt attccctgct gcaggtgtta ataatacacc agcaacattt
    12721 aatccacata attcactgac taattttaat tctttttcag ggtcttcatg attattcatg
    12781 ataatagtat aatagccttt agaagaaaat gtttcttcaa tgcctctaat aatccttgca
    12841 ttgtatggat tagaattatc acttaaaatg attccaacaa tttttgttct ttgctgacgg
    12901 aaacttcttg ccacttgatt aggaacataa tttaattttt ctgcatactc cagtatttct
    12961 tttcttctgc tttcagaaat atccggatgc ccgtttaaag ctttcgatac ggttgtaata
    13021 cttacattga gtgcatctgc tatgtctttt aaccttatca ttttcttcac tttcactcac
    13081 cctatttgaa ttattttaag gctgactgat gaaaatgtca ttctctactt tttacccttt
    13141 gctaaaaatt ttatatttct aatcactgac cataaataat aaaaaatatc atttaaccat
    13201 gcataacgct ggatctttta ctaagtcttt ttgcaacttc aaaactagta ggcatcctct
    13261 taacaacaca gccacctttt ttctcaataa ccaaagatgc cactgccgtt ccaaaaataa
    13321 ctgcttcttc cagactatcc atgtcatcca aactggcaat catacctgca gctaaagcat
    13381 cccctgcacc aacgagatta tattcttcct caggacattc tggtggaaaa acatctatat
    13441 gctttggtgc ttgtcctttg ctttgtatca aagctccttt ttcacccatt gtaataacaa
    13501 ccgactgagt tcccttatca ataaagtatt gactcattgt ccctagtggt atatccgggc
    13561 caatatattt caaatcttcc tcactggctt ttataatagt tgaatacata cataaatatt
    13621 caattaaagc ttttcctctt cttgtatcta tactaaaatg attataagaa gttgtcccgc
    13681 caaaaccgcc taaatccaca attactctct tacccatttc atgtaatttt ttagctatct
    13741 caatacttac ttcacaattc ataggacaaa taaaaaatgt ttctgccttt agatagtttt
    13801 ctggaatatc ttcaaactca attacaggag ctacttttgc atactcaaca ctttttttcc
    13861 catctgcatg aaaaattaaa ttattatgtg ttgtaaactt ataaggaatg aaaccttctg
    13921 tatcaacaat ctggaattgt tctaccatat ctttatcaag atcatcaccg atatagctta
    13981 caatccctac ttttcttcct aaccttgcca taataatact ggaataagca caaggactac
    14041 ctaacactgg acctattatc ttgtttggga aaataatcat ttcatttata atatttccta
    14101 acactacaat atctacattt atacccacag tggttcctcc caaataataa atcaagctca
    14161 aaaataaatt tttaa
//
LOCUS       DAOSUD010000056        13426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2751_length_13426_cov_23.738239, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000056 DAOSUD010000000
VERSION     DAOSUD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_13426_cov_23.738239"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(661..1281)
                     /locus_tag="PLG49_07805"
     CDS             complement(661..1281)
                     /locus_tag="PLG49_07805"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOQ17262.1"
                     /translation="MKKLDRSFYERDTLVVAQELLGKYIVHKIKGKELIARIVETEAY
                     KGPVDKAAHSYQNRRTPRTEVMFGPPGHAYVYLIYGMYLCMNIVTEEEGMPCAVLLRA
                     AEPVEGLDEMAQLRFNKTYKQLTRSQKINLTNGPGKLAMALNITREDNGLDLCAEKMF
                     ICESDSDSNFFIGRGKRINIDYAEEAADYLWRFYIKDNPYISKVKE"
     gene            complement(1347..2024)
                     /locus_tag="PLG49_07810"
     CDS             complement(1347..2024)
                     /locus_tag="PLG49_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOQ17263.1"
                     /translation="MQTDVVNYRFILTMVVANHGDGSKVLSEAKKIGIPGGTIFFGKG
                     TVHNHLLEMLGIDEEKKEIVILASEAAYEDELHERLNNKFNMHKPGKGIMVSLPINKL
                     LYTHNCVEEDSNRDLETGGKKEMEYQAIFIIVDRGLGHEVVDVATEAGAKGATIINAR
                     GSGIHEKEMFFSINIEPEKEIVMIIIKKELTDAIINAVKEKMKIDEPGRGIIFVMDVS
                     KASGLRD"
     gene            complement(2030..3502)
                     /locus_tag="PLG49_07815"
     CDS             complement(2030..3502)
                     /locus_tag="PLG49_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOQ17264.1"
                     /translation="MNLLKEKFRDVIMSVLPVSLIVILLNFTIAPIGSYLLWKFIIGS
                     VFIIMGLTIFLFGVELGIQPIGSLMGETITKSKKIWIVILFGFILGFFINIAEPDLQV
                     LARQVSNITSGVLSQISLVVVVSVGIGIMVALGLLRIVFSIPINKLLTVLYGIILLLA
                     VFSSESFHGIAFDSGGATTGSMTVPFILALGLGVASFKGGTEAEEDSFGLVGIASTGP
                     MIAVFTMSILSSIEKLTGVSSIQVGEERGILSSYIHEIPIVTYEVLMALLPFLILFIL
                     FNHLSFKLKRRQLRKIFLGLLYTFVGFILFLVGVNAGFMEAGRAIGQTIALIGKDWLV
                     ILTGFILGLTVILAEPAVYVLNEQIEDVTSGHIRKSLILFSLAVGVAVAVALSMIRIL
                     NPSVHLWHFLLPGYLLAILLTYFTPKLFVGIAFDSGGVASGPMTATFILAFSQGVAGA
                     IPGADVLLDGFGVISMVALTPLIAIQILGILFERKAAKRS"
     gene            complement(3787..4845)
                     /locus_tag="PLG49_07820"
     CDS             complement(3787..4845)
                     /locus_tag="PLG49_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ17265.1"
                     /translation="MGLFRRELLNVIEWNETRDDVLFWKWNNSEIKKGSRLIIRPGQD
                     AIFLYNGKIEGVFKEEGNYEIETQIIPFLSTLKGFKFGFNSGLRAEVLFVNTKEFLVR
                     WGTKNPINIPAQNMPGGIPIRAFGTFSVKIDDYISLIDKIAGVKKTFTISDIEERTLS
                     LLDQLLMKWIVKEGKNMFNLQVNAYEIAKGIKEDLDMELLKIGLTVTDLKISSFNYPE
                     QIQKMIEKNASYEMVGDIGRYQQIGIMNSLEKNPGGNMSSMLQAGVGLAMGAEMLNNI
                     KKSTTSNNPSVTPASSASVTLICKKCSQPLTSDMKFCSNCGEKVENFSAGGSKFCSQC
                     GSKLDANAKFCSACGAKL"
     gene            complement(4859..5416)
                     /locus_tag="PLG49_07825"
     CDS             complement(4859..5416)
                     /locus_tag="PLG49_07825"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOQ17266.1"
                     /translation="MAIFKRISKVVQANMNASGLKNNDKELEKFFKEIEDEIGKIKAE
                     KAKAETEKARAKRELDECNSEIKKLDKYIEKAKEAQEDETPFLQKKETLESKAIELER
                     KYEEILTKELKMRQTHDALLAQIEDLKARKEAIDIKLEKVKLQNKINDINSGSFASLE
                     EKVNRALDEAEAVAELNEFERENIF"
     gene            complement(5484..6848)
                     /locus_tag="PLG49_07830"
     CDS             complement(5484..6848)
                     /locus_tag="PLG49_07830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOQ17267.1"
                     /translation="MDLPKAFEDKMKILLKDEFIEFIKTFEEERFYGLRVNSLKISPE
                     EFKNKMPLNLSPIPWCKEGFYFGKDDKPAKHPYYHAGLYYIQEPSAMAAAEMLNVKPG
                     DKVLDLCAAPGGKSTQLGAKLEGKGVLFSNDISASRCKALLKNIEMAGIKNVVITNES
                     PEKLSKYLEGYFDKILVDAPCSGEGMFRKEPETIKSWKAERTEFFADLQKNILNYAAK
                     MLKPGGYILYSTCTFAPEENEGTIEAFLVNNPDFEIIPIDHNYNFAKGMPHWIGARRE
                     IEGTARLWPHKLMGEGHFLALLYKKDGKSFKMDKEDKKIKTNKGDLTDFYKFIDVNIK
                     GGIVENFEIHGDAIYLLPDQIPDLKGLRVVRSGWLLGFLKKNRFEPSQAMAMGLQKNQ
                     VVNFIDLSLEDPNVIRYLKGETLPIDGKEGWNLVCVDGYPLGWGKIQNNKMKNKYFAG
                     WRWM"
     gene            complement(6942..7214)
                     /locus_tag="PLG49_07835"
     CDS             complement(6942..7214)
                     /locus_tag="PLG49_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ17268.1"
                     /translation="METTEKRRFLRFINESDIKIKIGDNTFVYAMQDLSAGGFRIDYV
                     EGFTVGQIVDVVIDFECGQFVTQTKVIWQDKENGIGFEFVDTELFS"
     gene            7559..7795
                     /locus_tag="PLG49_07840"
     CDS             7559..7795
                     /locus_tag="PLG49_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17269.1"
                     /translation="MATTMSITEINIITVDKSEESWLIEGEIIFEEEFSSSFEGTYNT
                     ETEEFEELIIENEPKSYDKDDLIEKILKAVEEYE"
     gene            complement(7822..8508)
                     /locus_tag="PLG49_07845"
     CDS             complement(7822..8508)
                     /locus_tag="PLG49_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ17270.1"
                     /translation="MSIFAIGDLHLSGAVDKPMDKFGENWINHAEKIKNNWFKLVRNQ
                     DTVLIPGDISWAMNMEEAMIDLDFIHQLPGRKIFVKGNHDYWWKSIRKLNSLYEDMFF
                     IQNNFIEIEDFAICGSRGWLCPNDSEYTEHDKKIYNREVKRLELSLKEAQKAKSSRKI
                     VMMHYPPTNDKLEDSAFTALFEKYQVEKVIYGHLHGDKNKAGLMGNFNGVEYCLVSCD
                     YIDFKPVLIV"
     gene            complement(8536..9495)
                     /locus_tag="PLG49_07850"
     CDS             complement(8536..9495)
                     /locus_tag="PLG49_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410300.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOQ17271.1"
                     /translation="MTLKENLYRRYSSYLKEKYGEKVYKLPVNLPVSCPNRDGVLGYG
                     GCTFCGAEGTGFESLSNTISVAEQIKENMDYISKKYKAKKFIAYFQNFSNTYMPVKDF
                     ERLVKEAITDGVVQIAISTRPDCIRKEYLEILKSIQEHDKIDISIELGLQSVNYHTLL
                     KINRGHTLGEFIDAVLKIKNYPFEICTHLILNLPWDNETDVIENAKILSALSIQQVKL
                     HSLYIVDHTLMGMQYKNKEFDMISLEEYKKRVILFLEYLSPEIVIQRLIGRAPKENTL
                     FVNWNTSWWKIRDEIEEEMRKEGKYQGRLFNYLDGKGLKNFFE"
     gene            complement(9518..10336)
                     /locus_tag="PLG49_07855"
     CDS             complement(9518..10336)
                     /locus_tag="PLG49_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOQ17272.1"
                     /translation="MFKKLGIIIFSFILIFLSSSQVLYAYESNNVIDHLNFLTEEEVE
                     ELQDLIDEVKEDYRLELVIVITDDTQGKSSRDFADDYYDYNGYGVGSDKSGLLMLINM
                     DKREVWISTTGKAIDIFTDAKIDKMLDMIKPFLSDGDYYTASKTFIDYTRMYSRNDSS
                     SGYSSSGSTVIKDNSYMHRVRSYLSSPMTLLIAFVIALVVTLVIRFSSKGSVTVDSRT
                     YEETGAFRLNINQDIFLREITTKERIRSDTHSGGGSSTHIGSSGRSHGGGGRSF"
     gene            complement(10329..11363)
                     /locus_tag="PLG49_07860"
     CDS             complement(10329..11363)
                     /locus_tag="PLG49_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17273.1"
                     /translation="MSLVKEYRCPNCGAGVQFEPILQNWRCSFCNSEFDKSQLTMPLE
                     ESQEESEKVAENLNVYSCPSCGAQLIADETTSATFCLYCRSHNIIKDRFSGKFKPDQL
                     VPFRITKEQAKDIYTKWIKKKIFAPSIFKNKEEIDKITGVYVPYWLFDCNAQGYIEGT
                     ATRVRTWTSGDYRYTNTKYYRIVRSGSSRYSRVPVDGSKKLDDNLMYMIEPYDYAHMK
                     DFSMEYLSGFLAEKYDVDAGEAEKGMRQKVEKYLENRLKETVIGYSSFNVTQKSIDVH
                     DTSCKYAMMPVYLINNTYKDKDHLFIINGQTGKISGETPLSLVNQIAFGLGLMLIIWL
                     LIGFGGALFV"
     gene            complement(11560..12912)
                     /locus_tag="PLG49_07865"
     CDS             complement(11560..12912)
                     /locus_tag="PLG49_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ17274.1"
                     /translation="MVLATVTGCSKERISTGNESATEQTTTTEKENDENKDSEKKSLT
                     GEITYWHFNPDEGPAIARAFEKANPGVKIKDEITADTNGNYQTKVQAASRSGSLPDVY
                     AAESAFVKRFVNMPGGYMPLNDFEEIDEVTSQLVPFVLGIGTNEQGQLVALSHQATAG
                     GVGYKRPIAKQYLGTDDPTQIEEMLKPEKMIETGRKLKEVSGGKAILWAGIEELVPIY
                     LGAREHAWVEDGKFVIDEKVYELVDIAAQLRKEGLEGGLTRWEEAWANSIQDDTHMCY
                     AVPTWGINWIIDCRESADVMVMTTDDHPEPGTGGRWAICTPMPYNEGGTWFGISQNTK
                     NPELAWEFIKFIASPEYQESLAQAGDFVSNKNIIEKYKADDSFINKVINQNIYQVYSK
                     VSDQINGNVITQYDDTIRMALLDCLNSYLAGEISEEEMWKQFKERLQSELQGENIVFE
                     "
BASE COUNT         4299 a         2693 c         1716 g         4718 t
ORIGIN      
        1 taatattaat attacttcct tagtatatta aattatacac ttaataattt attaatctac
       61 aaaactaaac aattccgttg tataatttaa atataaatcc attataaaaa aggtatgata
      121 ggatttgaaa gaaaaaatct ttataaaaaa aatcgtggta cttttatcat taataactat
      181 cctaaattgg tctgtgcaaa ctgctttgaa tgaagaatat cattattctt cttcaaatca
      241 ttccgtttta ttatgctata catctattac agattctata ctgtctttta ctgattttaa
      301 gaataacaat gagagaaaag tattccattt aattttatat tcggcaaaca taatagttat
      361 ttttatatat ggttgtcaat atctgtataa gtaccagcga aaaaatcaaa ttttattaga
      421 taaaagaaaa atattatttg aactgttttc taattatttt gaagggagta tccataaatg
      481 atttttcacc tttcttattt tttataagaa ataagagggg ggtgaaagga tgtctgcaac
      541 ggacattttt gtagccgttt tgttaatact ttctgcaatt gctgctatta ttttagtaaa
      601 aaaatcaaaa aataatcaat aaaaaaggat taaaaaagaa tagagaagat aatatcttct
      661 ctattctttt actttggata tataaggatt atctttaata taaaatctcc ataagtaatc
      721 tgccgcttcc tctgcataat caatatttat tcttttacct cttccaataa aaaaattgga
      781 atctgaatca ctttcacata taaacatctt ttcagcacaa aggtctaatc cattgtcctc
      841 acgggtaata ttgagtgcca tagccagctt acccggacca ttagtcagat ttatcttttg
      901 tgatctggtt aattgtttgt atgttttatt aaatcttaac tgtgccatct cgtccaggcc
      961 ttcaacaggt tctgcagctc ttaataatac agcacacggc ataccctctt cttctgttac
     1021 aatattcata cataaataca tcccataaat caaatataca taagcatgac ccggaggacc
     1081 aaacatcact tctgttcttg gcgttctccg attttgatag ctatgggctg ccttatcaac
     1141 agggcctttg taggcttctg tttccacaat cctggcaatt aattccttac cctttatttt
     1201 atgaacgata tacttaccca acaattcctg ggctacaact aatgtatctc tttcataaaa
     1261 agatcggtca agttttttca tacgaacacc ctatctatta accattaaaa acttttaaaa
     1321 taataaaatt atcttaattt atctttctaa tcccttaatc ctgaagcttt tgaaacgtcc
     1381 ataacaaaaa taatccctct gccaggttcg tcgattttca ttttttcttt tacagcatta
     1441 ataatagcgt cggtcagttc tttttttata ataatcataa caatttcttt ttctggttcg
     1501 atattaatgg aaaaaaacat ttctttttca tgaataccag accccctggc attaataatc
     1561 gtagctcctt tagctcccgc ttccgtagct acatcaacaa cctcatggcc aagacccctg
     1621 tcaactatga taaaaatcgc ttgatattcc atttctttct tacctcctgt ttccaagtcc
     1681 ctattactat cctcttcaac acagttatgg gtatacaata atttattaat aggcaaagaa
     1741 accataatcc cttttccagg cttatgcata ttaaacttat tatttaatct ttcatgcagt
     1801 tcgtcttcat aggctgcctc tgaagctaaa atgacaattt cctttttttc ttcatcaatg
     1861 cccagcattt ctaataaatg attatgaacc gttccttttc cgaaaaaaat cgtccctcct
     1921 ggtatgccaa tcttttttgc ctctgataat actttactgc catccccatg attggctacg
     1981 accatagtca gaataaatct ataattaacc acatcggttt gcacttatct caactccttt
     2041 ttgctgcttt tctttcaaat agtattccta aaatttgaat ggctatcaaa ggggttaatg
     2101 ctaccattga aatcacacca aaaccatcta ataaaacatc cgctcccgga attgctcctg
     2161 ctactccctg agaaaaagcc aggatgaaag ttgctgtcat tgggcctgaa gcgacacccc
     2221 ccgaatcaaa ggctatccca acaaacaatt tgggtgtaaa atatgtaagt agaatagcta
     2281 aaagataccc tggcaaaagg aaatgccata aatggacaga tggatttaat atcctgatca
     2341 tagataaagc tactgcaaca gctacaccaa cagcaaggga aaataaaatc agacttttcc
     2401 tgatatgccc gcttgtaaca tcttcaattt gttcgtttaa cacatatact gccggctcag
     2461 ctaaaataac cgtaagtcct aaaataaaac cagttaagat gacaagccag tcttttccta
     2521 tcaaagcaat agtttgtcca atagctctgc ctgcttccat aaaacctgca ttgacgccta
     2581 ccaaaaaaag aataaaacct acaaaagtat agagtaaacc caggaagatt ttccgcaatt
     2641 gtctcctttt caatttaaaa ctgagatgat taaacagaat aaataatatt aaaaaaggaa
     2701 gcaacgccat taatacttca taagtaacga taggaatttc atgaatataa gaagagagaa
     2761 tccctctttc ttctccaact tgaattgacg aaacaccagt cagtttttca atagaagaaa
     2821 ggatactcat tgtaaaaaca gctatcatcg gaccagtcga tgcaatacca accagaccaa
     2881 agctatcttc ttcagcctca gttccacctt taaatgaagc aactccaaga cctaaggcaa
     2941 gaataaaagg aaccgtcata gaaccagtgg tagcgccacc tgaatcaaac gcaataccat
     3001 ggaaagattc tgatgagaat acagctaaga gtaaaataat tccatacaat acggttaaca
     3061 atttattaat aggaatacta aaaacaattc tcaaaagacc caaagccacc attataccta
     3121 ttccaacaga tacaacaaca actaaactga tctgagacaa gacgcctgaa gtaatattgc
     3181 ttacttgccg agctaaaact tgaagatctg gttcagcaat attaataaaa aagcccagaa
     3241 taaaaccaaa caaaataact atccagattt ttttactttt tgtaatcgtt tctcccatca
     3301 gggaacctat cggttgaatg cctaattcaa caccaaataa aaagattgtt aaacccataa
     3361 taatgaatac acttccaata ataaatttcc ataataaata actgcctatc ggtgcaatcg
     3421 taaagtttaa caggataaca atgagtgaaa ccggtaatac agacataatt acatccctga
     3481 atttttcttt tagtaagttc aatagacatc acttcctttc tttatagtta aaattaaaag
     3541 atacatatct attaatcatt tgataacact tgtcaatata atttatatta tattatagaa
     3601 ctcaacaaag cttcaagtcg gctatgagga attaccgtgc ctttccctta tcattgtaat
     3661 aatatgttaa gcttatcaaa aatatatcca tcaaagtgaa ctgctattgc accatcataa
     3721 ataaaaataa ccctgaatga acagggttat tctattgtaa gctgacgaat tattctgatc
     3781 tgaaatttat aattttgcac cacatgcact acaaaactta gcattggcat ctaattttga
     3841 accacattga gaacaaaatt tacttcctcc tgcagaaaag ttttccactt tttctccaca
     3901 gttactgcag aatttcatat ctgatgtaag tggctgggaa cattttttac aaattaaagt
     3961 tacagaagca gaagaagctg gtgtgactga aggattattg ctagttgttg attttttaat
     4021 attattaagc atttcagccc ccattgccaa acctactcca gcctgaagca tactggacat
     4081 attgcccccg ggattttttt ctaaagaatt cattatacca atttgttgat accgacctat
     4141 atctccgacc atttcataag aagcattctt ttctatcatc ttttgaattt gctcaggata
     4201 attaaagctg gaaatcttta ggtccgtcac cgttaaacca atcttaagca attccatatc
     4261 taaatcttcc ttaatgcctt tagcaatttc ataagcattg acttgaagat taaacatgtt
     4321 tttgccttct ttaacaatcc atttcattag caattggtct aaaaggctta aagtacgctc
     4381 ttctatatcg ctgatagtaa aagtcttttt aacgcctgca attttatcaa taaggctgat
     4441 ataatcatca atttttacac taaaagtacc aaaagccctg attggaattc ctcccggcat
     4501 attttgtgcc ggaatgttaa ttggattttt tgtaccccat cttactaaaa attcttttgt
     4561 atttacaaat aagacttctg ccctgagacc actgttaaaa ccaaatttaa aaccttttaa
     4621 agtgcttaag aaaggaatta tttgcgtttc tatttcataa ttaccttctt ctttgaatac
     4681 accttcaatt tttccattat aaagaaagat agcatcttgt ccaggtcgaa taattaagcg
     4741 gctgcctttt ttaatttcgc tattattcca tttccaaaat aaaacatcat cccttgtttc
     4801 attccattca atcacattaa gtaattctct tcgaaaaaga cccatttccc tttccccctt
     4861 aaaaaatatt ttctctctca aactcattta attccgccac tgcctctgct tcatccaatg
     4921 cacggttaac tttttcttca agacttgcaa aggagccaga atttatatca tttattttat
     4981 tttggagttt gactttttct aatttaatat cgatggcttc ttttctggcc tttaaatcct
     5041 caatttgagc cagtaaagcg tcatgggtct gtctcatctt caattcttta gtcaaaattt
     5101 cttcatattt tctttccaat tcaatggctt tactttctaa agtttctttc ttctgcagaa
     5161 aaggggtctc atcctcctga gcttcctttg ctttttctat atatttatcc aattttttaa
     5221 tttcactgtt acattcatcc agttctcttt tggcacgtgc cttttcagtc tctgcttttg
     5281 ccttttcagc ttttattttt cctatttcat cctcaatttc tttaaaaaac ttttctaatt
     5341 ctttatcgtt attttttaaa ccagaagcat tcatatttgc ctggactact tttgaaattc
     5401 ttttaaaaat cgccatcttt aacctcctaa aaacagttaa atattaaata tattctataa
     5461 tattacttat tagaattcaa ctattacatc catctccaac cagcaaaata cttatttttc
     5521 attttattat tttgtatctt gccccatcct aaaggataac catccacaca caccagattc
     5581 cagccttctt ttccatcaat aggaagggtt tcccctttta aatagcggat aacattagga
     5641 tcttctaaag ataaatcaat gaaattaacc acttgatttt tttgaagacc catggccatg
     5701 gcctgtgaag gttcaaaacg atttttcttt aaaaatccga gaagccaacc agacctgact
     5761 acccttaaac cttttaaatc cggaatttgg tctggtaata aataaatagc atctccatgg
     5821 atttcaaaat tttctactat cccgcctttt atatttacgt caataaattt ataaaagtct
     5881 gttaaatctc ccttatttgt tttaattttt ttatcctctt tgtccatttt aaaactttta
     5941 ccgtcttttt tataaagtaa tgctaaaaaa tggccttctc ccattaattt atgaggccat
     6001 aaccttgccg taccttctat ttccctcctg gccccaatcc agtgaggcat tcctttagca
     6061 aaattatagt tatggtctat tggtataatt tcaaaatcgg gattatttac cagaaatgct
     6121 tctatcgtac cctcattttc ttcaggcgca aaagtacagg tagaatatag aatataacca
     6181 ccaggtttca acattttagc ggcataatta aggatatttt tttgtaaatc agcaaaaaac
     6241 tcagtccttt ctgctttcca gctttttatg gtttcaggtt cttttctgaa catcccttct
     6301 ccagaacaag gtgcatcgac taaaatttta tcaaaatatc cttctaaata ctttgacaat
     6361 ttctctgggc tttcattggt aatgactaca tttttaatcc ctgccatttc aatatttttt
     6421 aaaagcgctt tacatcttga agcactgata tcattagaaa acaatactcc tttgccttct
     6481 aattttgccc ccaattgagt gctttttcct ccaggggctg cacataagtc taaaacctta
     6541 tctcctggtt ttacatttaa catttctgct gcagccatag cactaggttc ttgtatataa
     6601 tataagccag catgataata cggatgtttt gcaggcttat catctttacc aaaataaaaa
     6661 ccctctttgc accagggaat tggagataga tttaaaggca ttttattttt aaattcttca
     6721 ggactaattt ttaatgaatt aaccctgaga ccataaaatc tttcttcttc aaaagtttta
     6781 ataaactcaa taaattcatc cttaagaagg attttcattt tatcttcaaa cgctttaggt
     6841 agatccatta tgccttgctc ctttgttctt ttaaaatatt atacccaatt ctttttataa
     6901 attgtaaaaa gcgtgtgcaa agacacgctt tagaaaaact cctaagaaaa cagttcagta
     6961 tctacaaatt caaatcctat accgttttct ttatcttgcc atatcacttt tgtctgagta
     7021 acaaactgac cgcattcaaa atcaataaca acatctacaa tttgccctac agtaaaacct
     7081 tcaacataat caattctaaa cccgcctgca gaaagatcct gcatagcata tacaaaagta
     7141 ttatccccta tttttatttt tatatctgat tcatttataa aacgtaaaaa ccttcgtttt
     7201 tcagtagttt ccacgctttc acgctcctta acataagata tattaataaa cacttaatgt
     7261 caagattccg cccatatgtt cattttataa taattgtctg atatattcaa cataattaaa
     7321 ttcattaatt aaaattattc attataattt attaattaaa ctttccatgt atgtttttat
     7381 taaaattaca taaaacattg atatattcat atagtattaa tatcaataat ccttatgcgc
     7441 ataaaaatta aatagataaa taattctttg aatactataa ttatacacca attttaaaaa
     7501 taaataaagt taaaaaacta aattaatatc atttactttt tcaaaaagga ggtatcttat
     7561 ggcaactacc atgtccatta ccgaaatcaa tattattact gtagacaaat cagaagaatc
     7621 ctggttaatt gaaggcgaaa ttatatttga agaagaattc agcagttctt ttgaaggcac
     7681 atataatact gaaacagaag aatttgaaga actcattatt gaaaatgaac ctaaaagtta
     7741 tgataaagat gatttaatag aaaaaattct aaaagctgtt gaagaatacg aataataaat
     7801 aaagtcccct aataagggac tttatacaat aagaacaggt ttaaaatcaa tataatcaca
     7861 agaaaccaga caatattcta ctccgttaaa attgcccatt aaacctgctt tgtttttatc
     7921 tccatgcaag tgaccataaa tcaccttttc tacctgatat ttttcaaaca atgccgtaaa
     7981 agcagaatcc tccaacttat cattagtagg aggataatgc atcatgacta tttttctgct
     8041 gcttttagcc ttttgtgctt cttttaatga aagctccaga cgttttactt ctctattgta
     8101 tatttttttg tcatgttccg tatattcact gtcattaggg caaagccatc ccctgctacc
     8161 acaaatggca aaatcttcta tctctataaa attattttgg ataaaaaaca tatcttcgta
     8221 taagctgttt aattttcgaa ttgatttcca ccaataatca tgatttcctt taacaaatat
     8281 ttttcggccg ggcagttgat gtataaaatc taaatcaatc atagcttctt ccatattcat
     8341 tgcccaggaa atatccccgg gtataagaac tgtatcctga tttctaacca gcttaaacca
     8401 attgttcttt attttttctg catggtttat ccaattttct ccaaacttat ccatgggttt
     8461 atccactgcc ccactcaaat gcaaatcacc tatggcaaag atggacatag taatgtctct
     8521 cctttttagt tatgcttatt caaaaaaatt ttttaacccc ttaccatcta aataattaaa
     8581 taatctcccc tggtattttc cctcttttct catttcttct tctatctcat ccctgatctt
     8641 ccaccaactt gtattccagt tcacaaacaa agtattttcc tttggtgctc ttccaattaa
     8701 tctctgtatg acaatctcag gagaaagata ttcaagaaac aggatcactc tttttttgta
     8761 ttcttccagg cttatcatat caaattcttt attcttatat tgcattccca taagcgtatg
     8821 atcaacgata tataaagaat gaagttttac ctgctgtata gaaagagcag acaagatttt
     8881 tgcattttca atcacatctg tttcattatc ccatggcaaa ttcaaaatta aatgtgtaca
     8941 tatttcaaaa ggataatttt taattttaag aacagcatct atgaattccc ctaaagtatg
     9001 tcctcggtta atctttaaaa gtgtatgata attcacagat tgaagaccta actcaataga
     9061 tatatcaatt ttatcatgct cctgtatgct ttttaaaatc tctaaatatt cctttctaat
     9121 gcaatcaggt cttgtcgata tagcaatttg aacaacacca tctgtgatgg cttcttttac
     9181 aagcctttca aagtctttta caggcatata agtattactg aaattttgaa agtaagcaat
     9241 gaattttttt gctttatatt ttttactgat ataatccata ttttccttaa tttgttcagc
     9301 gacagaaatg gtattggata aactttcaaa acccgtacct tctgctccgc aaaaagtaca
     9361 gcccccatag cctaagacac catccctgtt gggacaacta acgggcaaat taacgggcag
     9421 cttgtatact ttttcaccat atttttcttt taaatagctg gaatatcttc tatacaaatt
     9481 ctcttttaaa gtcattgcct tcaccgtctt aactaaatta aaaacttctt ccaccgccac
     9541 catggcttct tccgctactt ccaatatggg tgctgcttcc tccgcctgaa tgcgtatctg
     9601 aacgaatgcg ttcttttgtc gttatttctc ttaaaaagat atcctgattt atatttaacc
     9661 taaaagctcc agtctcttca taagttcgac tgtctactgt aacagaacct ttacttgaaa
     9721 accttataac aagggttact actaaagcta taacaaaggc aataagaaga gtcataggtg
     9781 aagataaata tgatcgcacc ctatgcatat atgaattatc tttaataacc gtagagccag
     9841 aactgctata tccagaggaa gaatcatttc tactgtacat tcttgtatag tcaatgaaag
     9901 ttttcgaggc tgtataataa tctccgtcag aaagaaaagg ctttatcata tcaagcattt
     9961 tgtcaatttt agcatccgta aagatatcga ttgccttacc tgttgtggat atccacacct
    10021 ctcgtttatc catattaata agcatcaaaa gtcctgattt atcactgcct accccataac
    10081 cattataatc atagtaatca tctgcaaaat ctctggatga cttcccttgg gtatcatctg
    10141 ttataacaat aaccaattct aaacgataat cttcttttac ctcatcaatt aaatcctgta
    10201 attcttcaac ttcctcttca gtaaggaaat tcagatgatc tattacgttg ttgctttcat
    10261 aagcataaag gacttgactg gaagaaagaa aaatcaaaat aaaactaaag ataattatcc
    10321 ctaatttttt aaacaaataa agcaccccca aatccaatta acagccaaat aataagcatt
    10381 aatcccaagc caaaggcaat ttgatttact aaacttaatg gcgtttctcc agaaatcttt
    10441 cctgtttgac cgttaataat aaaaagatgg tctttatcct tatatgtatt attaattaaa
    10501 taaactggca tcatagcgta cttacaagac gtatcatgta catcaatgga tttttgagtg
    10561 acattaaaag aagaataacc aataactgtt tcctttaatc ggttttcaag atatttttct
    10621 actttctgtc tcatgccttt ctctgcttca ccagcatcga catcatattt ttcagccaaa
    10681 aagcccgata agtattccat tgaaaaatct ttcatatgcg cataatcata aggctctatc
    10741 atatacatta aattatcatc taatttcttt gaaccatcaa caggcactct ggaatatctt
    10801 gagcttccgg atctcacaat cctgtaatat tttgtattcg tatagcgata atctcctgac
    10861 gtccatgttc tcactcttgt agctgttcct tcaatatatc cctgggcatt gcagtcaaat
    10921 aaccaatagg ggacataaac gcctgtaatt ttatcaattt cttccttatt tttaaaaatg
    10981 ctgggcgcaa atattttttt cttaatccac tttgtataaa tatctttagc ctgctcctta
    11041 gtaattctaa aaggtacaag ctgatctggt ttaaatttgc ctgaaaatct gtctttaata
    11101 atgttatggc tcctgcaata taaacaaaaa gttgcagaag tcgtttcatc tgcaatcaac
    11161 tgagcaccgc agctgggaca ggaataaaca tttaaatttt ctgctacttt ctctgactct
    11221 tcctggctct cttcaagcgg cattgtaagc tggcttttat caaattcact attacagaag
    11281 ctacaacgcc aattctgtaa aataggctca aattgaactc cggcaccgca attaggacac
    11341 ctgtattctt ttaccagact cattttattt ccccctccaa tacacatata tttatattat
    11401 acaccatctg attcaatcca aatacacaaa tatcatcata atattaacaa atttttataa
    11461 taaagttcaa tattttataa aaaattatgc cttattagca tttgctaata aggcataata
    11521 tttattgtta aaaagatagc atggttttac tgatgataat cattcaaata caatattttc
    11581 tccttgtaat tcagattgca atctttcttt gaattgtttc cacatttctt cttcactgat
    11641 ttctccagca agatatgaat tcaggcagtc aagtaatgcc attctgatag tatcatcata
    11701 ttgcgtaata acgttaccat taatttgatc agatactttg ctatatactt gataaatgtt
    11761 ctgatttatt actttgttaa taaaactgtc atctgctttg tatttttcaa taatgttttt
    11821 attacttaca aaatccccag cttgggctaa actttcttga tattccggac ttgctatgaa
    11881 tttaatgaat tcccatgcaa gttctgggtt ctttgtgttt tgagagattc caaaccatgt
    11941 accaccttca ttatatggca ttggtgtgca gattgcccat cttccacctg ttcctggttc
    12001 tggatgatcg tctgttgtca ttaccattac gtctgctgat tcacgacagt cgataatcca
    12061 gttgattccc catgtgggta ctgcataaca catgtgtgta tcatcttgaa tggaatttgc
    12121 ccatgcttct tcccatcgtg tcagtccgcc ttcgagccct tctttcctta attgtgctgc
    12181 tatatcaact agttcataca ctttttcgtc aatcacaaat ttaccgtctt caacccatgc
    12241 atgttcacga gcaccaaggt aaataggaac taattcctct attcctgccc acaaaattgc
    12301 ttttccacca ctgacttctt ttaattttcg tcctgtttca atcatttttt ctggttttag
    12361 catttcttca atttgtgtag gatcgtctgt tcctaagtat tgttttgcga ttggtctttt
    12421 ataaccaaca ccaccagctg tggcctggtg agataaagct accaattgac cttgctcatt
    12481 tgtaccgatt cctaaaacaa aggggacaag ctggctggta acttcatcaa tttcttcaaa
    12541 gtcgtttaat ggcatatatc caccaggcat attaacaaaa cgttttacaa aggcactttc
    12601 tgctgcatat acatcaggca gactgccaga cctggaagct gcttgtacct tagtctgata
    12661 gttcccattt gtgtctgctg tgatttcatc ttttattttt actccagggt tagctttttc
    12721 aaaggctctc gctattgctg ggccttcatc tgggttgaag tgccagtagg tgatttctcc
    12781 tgtaagactc tttttttcac tgtccttatt ctcatcattt tctttttcag tggttgtagt
    12841 ctgttctgtc gctgattcat tacccgtaga aattctctcc ttgctacaac cagtcaccgt
    12901 tgccagtacc atactcaagc aaagcagtaa agtcatagtc ttttttaaaa gactcatata
    12961 aatactcctc ccctttaata atttatataa aaggcataat gccttttata tcagtcgata
    13021 aagttacttg ctcaaaattt gaaattaaat tcatttctat gtatcctaca accatattaa
    13081 aaattaataa taatttaaat ttaatttttt caattctgtt ttacttttgt gattatttta
    13141 cttttctcat taatccaatt caaggtattt tataaaaatt atataaattt ttaacaataa
    13201 tataggaaat tttaacaaat gcttaacaaa ttatgtttga tttgtataaa ttgcgaccat
    13261 ataagttact tcttattata tatatttaac ctatttattt ctttgaaaaa ttgttcacta
    13321 tacaatgtac aatcttttat aaatccctta gaattttttt aaattttgca agtgtcaccg
    13381 ccggggtata attgacccat agcgccggac agaaattgac ccacac
//
LOCUS       DAOSUD010000057        13217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2791_length_13217_cov_26.061769, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000057 DAOSUD010000000
VERSION     DAOSUD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_13217_cov_26.061769"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..392
                     /locus_tag="PLG49_07870"
     CDS             126..392
                     /locus_tag="PLG49_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOQ17275.1"
                     /translation="MFIRQDLSQIRKDIQDCIGTRVKLKTNKGRRKTVINEGILEDAY
                     PSVFVVKVKNSFENSFRRVSYSYTDILTQTVELSFYNDSENKVN"
     gene            479..2050
                     /locus_tag="PLG49_07875"
     CDS             479..2050
                     /locus_tag="PLG49_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOQ17276.1"
                     /translation="MSLELIKETVEFDQIIGKESVQIIVEGDIIVPDIKPDILKVLQI
                     DGKIDMGQLDISADRLNYKGQVKVNILYAAEGSERSIHTMSASLSMDDFINMDGIKKD
                     MSYQLDYFLEHLDYSLINSRKLNVRALITLNAKVVDKKQNAVVVGTDSRAPIQVQTRD
                     FNYYKTVGSKQDKIIIKDELTVPAGKANIQEILKNDISLSNKEVRTGDDTITVKGYLN
                     IRTLYASSMGEKEIEYMEHSIPYNGSIDCPGAGSEEGIYCYVDIKVSNYHVQVKPDLD
                     GEERILDIEVELQVNVRVISVENLKIIDDAYCPGKEILIRKEKIPYQRLVNKLKEVIT
                     LNEVINIDSDLPDINYIYNVEVRPKIEEIQLFEDKIALEGMAEVKMIYMSEDEEKPVC
                     VYESMLPFKKNIVAEGASLEKKLEVKVDVDDIIYSRESQKDFQVNIILNLDVDIIDEQ
                     KVEIIVDMTEKDLDPDVLLNMPSLIIYMVQCGDTLWKIAKKYNTTVEELVAINDIENP
                     DKIYPGQKLLIIKKI"
     gene            2222..3094
                     /gene="ispE"
                     /locus_tag="PLG49_07880"
     CDS             2222..3094
                     /gene="ispE"
                     /locus_tag="PLG49_07880"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOQ17277.1"
                     /translation="MHEIELKARAKINLALDVLSRREDGYHNVRMVMQTVDLYDYITI
                     RKTKSPGIKLKSDYKWLPEDERNIAYKAAKLMFDSYSIDEGILINIHKKIPVAAGLAG
                     GSADAAAVLVGLNKLFDLKKTKKELMDLGYQLGADVPFCILRGTALAEGIGEKLTQLP
                     SMPFCYVVIAKPLFSVSTAYVYQNLDLNNLSYHPDIEEVIKGIQMGDLEHIAKNLGNV
                     LETVTIKEHPEINDIKKKMIEFGALGALMSGSGPSVFGLYKDKKEAQAAYTELKLHSQ
                     AKDVFIATIFNRER"
     gene            3097..3780
                     /locus_tag="PLG49_07885"
     CDS             3097..3780
                     /locus_tag="PLG49_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ17278.1"
                     /translation="MDKNELDINLNEYLPLRDVVFNTLRKAILSGSLKPGERLMEKQL
                     AEKMGVSRTPVREAIRKLELEGFVVMVPRKGAQVAEITEKDIQDVLEVRGALEALAVK
                     IACEKMKKEDLDHLKEIMKEFNEASEKKDIDKVIEKDVEFHDVIYQATNNEKLIQIIN
                     NLREQIHRYRVAYLKSFADYKFIGEEHKQIIRAIENKDQNLAEQIANEHIKNQEKTVI
                     NYIKQGSKN"
     gene            3907..4143
                     /locus_tag="PLG49_07890"
     CDS             3907..4143
                     /locus_tag="PLG49_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17279.1"
                     /translation="MKSIGWEWIMEWFINKMVSIYSIWPMILTVGIGFFTLFKDAQIL
                     KDHNCLREAKWARILGYIYIFAGSGFYILFKILS"
     gene            4209..5800
                     /locus_tag="PLG49_07895"
                     /pseudo
     CDS             4209..5800
                     /locus_tag="PLG49_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
     gene            5810..6909
                     /locus_tag="PLG49_07900"
                     /pseudo
     CDS             5810..6909
                     /locus_tag="PLG49_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
     gene            6899..8050
                     /locus_tag="PLG49_07905"
     CDS             6899..8050
                     /locus_tag="PLG49_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOQ17280.1"
                     /translation="MKNKLLIIFLVISLFLTGCWDNININRRAFVISMGIDKFVFDEE
                     KDDPKLNNDLSRYRIAFSIPNLPKFVGQQGVSDEPKYTRVSVGTTITNAYKSLESRIN
                     KSIYFGHTKSLLFGEELLKDKVLLKQTMDALQRNSDFGKSIPVVAVEGSVVDALEVLP
                     DTQPMVGIYIMELFENNKERRSFIEEKELGNLLQDFEENKTGLIPRLIVGEKDLKLEG
                     AAIIKDYELQGWLDEKYLKGILWLLKKSYRLEISVPYHDYYIAYDVSDSETKIEFSGD
                     SQDLSCKISIETEGSIDEYILDEEMFQAEEIRNLEKAVEKEIEKEVLDSIEILQKKYK
                     TDVIEIGKRLERQKPEIWKEIKDNWDEVFENMDFTVDVKVYARRVGQTK"
     gene            8115..8822
                     /gene="spoIIR"
                     /locus_tag="PLG49_07910"
     CDS             8115..8822
                     /gene="spoIIR"
                     /locus_tag="PLG49_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOQ17281.1"
                     /translation="MKKINVIHWFSKEKTILLLSLICGFLITAFILHASKEYSETIQS
                     GLAANVIRFHVIANSDSKEDQEIKEKVRDAILNEMAPVLSDSDSIEETRNLILDNRDK
                     IKKIAEEVIHKNGKSYPVEVTLGPAVFPTKQYGDIVLPTGEYEALRVSIGKAQGKNWW
                     CVMFPPLCFVDVTHGVVPEESKTDLRKVLTDEEYKIIITQVTNEEEIPVKIKFKIVKW
                     WQERKLSKGHHFAQKRF"
     gene            9093..9332
                     /locus_tag="PLG49_07915"
                     /pseudo
     CDS             9093..9332
                     /locus_tag="PLG49_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
     gene            9396..9962
                     /gene="sleB"
                     /locus_tag="PLG49_07920"
                     /pseudo
     CDS             9396..9962
                     /gene="sleB"
                     /locus_tag="PLG49_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
     gene            10022..10726
                     /gene="sleB"
                     /locus_tag="PLG49_07925"
     CDS             10022..10726
                     /gene="sleB"
                     /locus_tag="PLG49_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HOQ17282.1"
                     /translation="MKAKKIILLMVNLIMFLNLAYSFWIYKVTPKAVDTITYTWGSEG
                     DVVRQIQQKLKQWGYYEGNVDGKYGYKTWEAVKAFQSKNGLKADGIAGKQTLEKMGIQ
                     VKDSSRKQGGDNVASNKDLMLMAAAIHGEGRGEPYEGQVAIGAVILNRVRHSSFPNTI
                     AGVIYQPGAFDAVADGQINLEPGDSAIKAARDALNGWDPTGGAIYYWNPATAKSKWIW
                     SVPITKRIGKHVFGKK"
     gene            10746..12143
                     /gene="ypeB"
                     /locus_tag="PLG49_07930"
     CDS             10746..12143
                     /gene="ypeB"
                     /locus_tag="PLG49_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOQ17283.1"
                     /translation="MEEKDNRLSQWKRLSGGSFLIALGLLLGFGIYQYKQKLNYRQYL
                     QNRFQESFYEATTYVDNMENLFTKVRLTKSPDQSAPLFAQIWREASSAQDNLAELPYD
                     HNTISKALKFLSQASDFSFSMTNKAIEGFELDEEDWKKVDQLKKYAKILSDELNKIKQ
                     EVNDGKRIDWEEIESEGEKRLAKGNTKAITGSFSEISKQFEDIPTLIYDGPFSDHIER
                     MEPRMTRNKKKISKEEGKKVVEEFIGKDKVKSIKQTGETDTKVEHTIPVYSYQVVLRD
                     QDEPTISIDVTQHGGLVLWMLNYTEKESTEEKLSLEEARKKAEKFLEERNYPNMKASY
                     YEKVDGTVVINFAPYENKVIMYPDLIKVKVAMDDGEIIGFESLGYIMMHHDRKLPEIK
                     ITEEEARDKITKDFKVQQVNMAVIPLESKREVLCYEFKGEFDNQLFLIYINAESGKQE
                     KILQILQSKNAVLTK"
     gene            12357..12662
                     /locus_tag="PLG49_07935"
     CDS             12357..12662
                     /locus_tag="PLG49_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17284.1"
                     /translation="MAFENGLFSFLLIMIISFILTVIGIILLVIFKNKTNGLRFIGFL
                     LISIAMIMILFSKKLCLDTVRVKETVDVAPMTELKFSSILKVLKRLWKALNHLTNWK"
     gene            12626..13075
                     /locus_tag="PLG49_07940"
     CDS             12626..13075
                     /locus_tag="PLG49_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17285.1"
                     /translation="MESIESFDELEVNTIDGVNIAMPVRTTDGIVEMLTGVDPKGAYL
                     MVNIYVYGSVKGANESYEHNYKNLGKDYGIMTSGGSNSDRYFVTYKNQLRLSGDALYL
                     LTDQYVTYVYFQKRNMIIKLMETKRDDTDSKINDYIKLLSDKLSELE"
BASE COUNT         4689 a         1743 c         2736 g         4049 t
ORIGIN      
        1 agtataacat aattactatt atctattgac agttaatgag agattttggt ataattaaaa
       61 atttattgac atctcagctt atttatgata cactaattta agtgacatag gatgaggtga
      121 tgtaaatgtt tataaggcaa gacctttccc aaatcagaaa ggacattcag gactgcattg
      181 gtaccagagt aaagcttaag accaataaag gaagacggaa gacagttatc aatgaaggaa
      241 tacttgaaga tgcttatcct agtgtttttg ttgtaaaggt aaaaaactct tttgaaaatt
      301 cttttagaag agtttcttat agttacacag atattttaac acaaaccgta gagctttctt
      361 tttataatga ttccgaaaac aaagtcaatt aatttgattt tgtttttttt attttgtcat
      421 aaattcctta ggattagtca tacatttata tagacaaagg tcctaaggag ggaaatatat
      481 gtcattggag ctaattaagg aaacggtaga atttgatcag attataggaa aagaaagcgt
      541 acagattatt gtagaaggag atattattgt acctgacata aaaccagata ttttaaaggt
      601 tttacaaatc gatggaaaaa tagatatggg gcagctggat atttcggcag acaggcttaa
      661 ttataaaggt caggttaaag tcaatatcct ttatgcagcg gaagggtcag aaagatcgat
      721 acatactatg tcagcaagcc tatctatgga tgattttatt aatatggacg gaattaagaa
      781 agatatgtct tatcaattgg attacttcct ggagcatttg gattattcat taattaatag
      841 ccggaaactt aatgtacgtg ctttaatcac tttaaatgcc aaggttgtcg ataaaaaaca
      901 aaatgctgtg gtggttggta cagacagtcg ggctcccatc caggttcaga cccgggattt
      961 taattattat aaaactgtag gcagcaaaca agataaaatc atcataaaag acgaattgac
     1021 ggttcctgca ggcaaggcaa atatacaaga aattttaaaa aatgatatca gcctttctaa
     1081 caaggaagtc agaacaggtg atgatacgat tactgtaaaa ggatatttaa atataagaac
     1141 cctgtatgca agcagtatgg gagaaaaaga aattgagtat atggaacata gtattcctta
     1201 taatggcagt atcgattgcc cgggggcagg aagcgaagaa ggtatttatt gttatgtaga
     1261 tattaaagta tcgaattatc atgtgcaagt taaacccgat ttagatggag aagaaagaat
     1321 actagatatt gaagtagaac ttcaagtaaa tgtcagagtc atttctgttg aaaatttgaa
     1381 aattattgat gatgcttact gtcccggaaa agaaatactt atcagaaagg aaaagattcc
     1441 ttatcaaaga cttgttaata agttaaaaga agtcataaca ctcaatgaag ttattaatat
     1501 tgattcggat ttaccagata tcaattatat atataatgtc gaagtcaggc caaagataga
     1561 agaaattcaa ctttttgaag ataagattgc tttagagggc atggcagaag taaaaatgat
     1621 atatatgtct gaagatgaag aaaagcctgt ttgcgtctat gaaagtatgt tgccctttaa
     1681 gaaaaatatt gttgcagaag gggcttcttt agaaaagaag ctggaagtta aagttgatgt
     1741 agacgatatc atttatagcc gggaatctca gaaagatttt caggttaata ttattcttaa
     1801 tttagatgta gacattattg atgaacaaaa agttgaaatt attgtcgata tgacagagaa
     1861 agatctggac ccagatgttc ttttaaatat gccaagtttg attatttata tggtgcaatg
     1921 tggagatacc ttatggaaaa tagcaaaaaa atataataca acggttgaag aactggtagc
     1981 cattaatgat atagaaaatc ctgataagat ctatcctgga caaaaattgc ttattattaa
     2041 aaaaatataa aaaaagacgg ggaaacctgt ctttattttt ttctgcttat tgaaaatcat
     2101 ttgaaagtat tgtaacttcg cttaaaacat ttagtattgg gaaaatcctt atagttgact
     2161 tgcaaatcat atggtgtata taatattaag tatacaacct tgtggaaatg atgtgatgat
     2221 gatgcacgag attgaattaa aggcccgggc aaaaatcaat ttggccctgg atgtattaag
     2281 caggagagaa gatggttatc ataatgtcag aatggttatg caaacggtcg atttatatga
     2341 ttatataaca atcagaaaga caaaaagtcc agggataaaa ttaaaaagcg attataaatg
     2401 gctgcccgaa gatgaaagaa acattgctta taaagcagca aaattgatgt ttgatagtta
     2461 tagtatagat gaaggaattt taataaatat tcataaaaag atacctgttg cagcaggact
     2521 tgcaggagga agtgctgatg cagcagcagt tttggttgga ctgaataaat tatttgattt
     2581 aaagaaaacg aaaaaagaat taatggattt aggatatcaa ttaggtgctg atgttccgtt
     2641 ttgtatttta agggggacgg ctttagcaga aggtatcgga gaaaagctta cccaattgcc
     2701 ttcaatgcct ttttgctatg ttgtcattgc aaaacctttg tttagtgttt caacggcata
     2761 tgtataccaa aatcttgatt taaataattt atcttatcat cctgatatag aagaagtgat
     2821 taagggaatt cagatgggag atttagagca tattgcaaaa aatcttggta atgtattaga
     2881 gacggtaaca ataaaagagc atcctgaaat taatgacata aagaaaaaaa tgattgaatt
     2941 tggagcattg ggtgctttaa tgagtggtag cggtccttct gtttttggac tttataaaga
     3001 taaaaaagaa gcacaggctg cttatacaga gcttaagctt cacagccagg ccaaggatgt
     3061 ttttatcgct acaatattta atagggagag ataggaatgg ataaaaacga attagatatt
     3121 aacttaaacg aataccttcc tcttagggat gttgtattta atacacttag aaaggctatt
     3181 ttatctggaa gtttaaagcc cggagaaaga cttatggaaa agcaattggc agaaaaaatg
     3241 ggtgtaagcc ggactcctgt cagagaagct ataagaaaat tagaattaga aggttttgtt
     3301 gtaatggtac ccaggaaagg agctcaggtt gcagaaataa ctgaaaaaga catccaggat
     3361 gtattagaag tccggggagc tttagaagct ctagccgtta aaattgcctg tgaaaaaatg
     3421 aaaaaagaag atttagatca tttaaaagaa attatgaaag agtttaatga ggcttctgaa
     3481 aagaaagata ttgataaagt tattgaaaaa gacgtagagt ttcacgatgt catttatcag
     3541 gcaaccaata atgaaaagct tattcaaatc attaataatt taagagaaca aattcaccgc
     3601 tacagggtgg cttatttaaa aagctttgca gattataaat tcattggtga agaacacaaa
     3661 caaattatac gtgccattga aaataaggat cagaatttag ctgagcaaat agccaatgag
     3721 catattaaga atcaagaaaa aactgtgatt aattatataa agcaagggtc aaagaattaa
     3781 cctttaggaa aaatcctaaa ggttttttta tgggtagttt cagggcagag gggtgaaagc
     3841 gatatatgat aattttagct tttatgaata aaatgcaaag aaagtacaaa aaatgaagta
     3901 aacaagatga aaagcattgg gtgggaatgg attatggaat ggtttattaa taaaatggtc
     3961 agtatttatt caatctggcc tatgatttta acagtaggaa taggtttttt tacccttttt
     4021 aaagatgcac aaattttaaa agaccataat tgcttaaggg aagcaaaatg ggcaaggata
     4081 ttaggctata tttacatatt tgcaggaagc ggtttttata tattattcaa aatattaagt
     4141 taaaagaatt atatttgtta aattaaaata aaaaattagc aaaatttagt actcaatagg
     4201 aggctaaaat gggaattaaa gactggttta aaaataaaac ggaaaggaaa gaacaatggg
     4261 aagatgcatt ccatgatgaa gataataaaa ttaccattcc tctttcaaaa aagttagatg
     4321 aaaatattaa aaacttagag attttatttc aggattgcgg tgactttgtc agaagaaagt
     4381 ttcctgttgg ggttaataaa gaaattaatg tttttattgc ctatattgat ttaatgatag
     4441 acagaagaat tattgaagaa tctttgctgg aacagcttat tgttcaaatc cgcagcgttc
     4501 cgcctcaaat ggacaggata aagacatcta tatatgaatt tatccgggac ggaggtttgg
     4561 ccactgcaga tataaaagaa gtagaaagcc tggatgaagc agttcttgcc atattatccg
     4621 gtgataccat tttaattatt gatggttatg caaaagttat agtgatatct acgaaaggct
     4681 ggcctaaccg cggcattcag gaattggatt cggaagttgt tatcagaggt tctaaagaag
     4741 gctttacaga agccctccgg atgaatactg ttttgattag aagaagaatc cgggatacaa
     4801 aactaaaagt gaaacaatta caaataggaa ccagaagcag gacggacatt gccattatgt
     4861 atctggatga tatcgtaaga aaagatattc ttgaggattt ggaaaagcgt ttagacagtt
     4921 ttaagattga cagcatatta gatagttctt atattgagga acttatacaa caggactgga
     4981 tgtctccttt tccgcaaata caggcgacac aaaggcctga caaagcagct tctgcaattc
     5041 ttgaaggaag aattgccatc gttgttgaca attctccttt tgtgctttta attccgtcaa
     5101 cgattaataa tttctttcaa tcctctgaag attattatga cagatggaca attatgagtt
     5161 ttacaagact gatccgtttt attgcagctt tttttctgtt gggatacctg gcttttatat
     5221 cgctttaacg gattatcatc cttctatgat tccgactcct ctttccatgg ctattgctgc
     5281 atcaagagaa caagtgcctt ttccaacggt ggttgaagtg gttttaatgg aattagcctt
     5341 tgaattacta agagaagcgg gaacccggct gccgggaccc ataggaagca cgattggaat
     5401 tgttggcggt attattatcg gacaagctgc agttgaagca aaaattgtaa gcccggttgt
     5461 tgttattatc gtagcagtta ctgccatttc cagctttgca attcctgata attccctgac
     5521 agtaggcttt agaattgtaa agtacttgat cattgctact tctgctattt taggtttata
     5581 tggctttttg ctgggaattc tgattgttct tattcatctt gtatccttaa aaagctttaa
     5641 tattccttac ttatatcctt atgtttcagc aggaataaca ggatataatg atttaaaaga
     5701 ttcgattttc agacttcctt tatttatgat gtataaaaga cctgtttatt ccagacatga
     5761 gcaaagaatc cggttcagaa tgaaaaagaa aaatgagtag gaggatatta tgttttcaaa
     5821 aaatgaaaag atctgtatca gacaactcga aagcctgctc attctggatt tatacagcag
     5881 aggtgtattg tttttaccaa gacttgctgt agaagctgcc aatcaggacg gttggatttg
     5941 ccttatagga gcaactattt tagcagcatt ttatggtttt attattacaa ggctggtaag
     6001 gcgttttcct aatgatactt ttgtagaatt ttctgcaaaa atattgtcca agcctgtagg
     6061 aatggtttta agcctcctct tttttgttaa attaataata tctgctgctt ttgaattaag
     6121 aatatttgga gaagtggtta aacaaatcct attgcctcag acgcctatag aagttattat
     6181 tgcttctatg cttcttatag cttcttactt ggttagaaaa ggctttgagg caagggcaag
     6241 attaggggaa attctttttt tattgttttt ataccgctta tttctgtgtt aatttttgtt
     6301 cttccagatg ctgattttgg taatttacaa ccctttttaa cgacttcttt tactcatttt
     6361 ttaaagagta gttatgattt gagccttact tatattgcca tagaattact tttattagta
     6421 ggacctttta taaatgatta tagaaaatta gaaaaggctt ctgttatttc catttttttt
     6481 gtaggtattt taaatgtgat ttcagcaatt atagttttgg ctgtctttgg agtagtgcaa
     6541 accaagaggc aattattgcc ttttatggtt ttaatgggaa tagtagaaat tccgggagcc
     6601 tttattgaaa ggcaagaaat tttaatgata attttttgga ttttatctga tttttctttt
     6661 ataaatgcta gtctttattt ttcgagcctt attataaaaa gagtgtcaaa aatagaggat
     6721 cagggctttt tagtacttcc cctggtacct attgtatatt taatttctct atttccggat
     6781 aatatcgtac agacaaccga ctggattttg gtgttaagaa gttttagaat tctatttatg
     6841 ttttttattc ctcttgttct tttattggtt gcgagattaa ggaagttggg tgagactgtt
     6901 gaaaaataag ctgttgatta tatttttagt gatttctcta ttcctgacag gttgctggga
     6961 taatataaac attaatagaa gagcctttgt gattagtatg ggtattgata aatttgtttt
     7021 tgatgaagaa aaagacgatc ccaaattaaa caatgatttg tcacggtaca gaattgcttt
     7081 ttccattcct aatcttccta aattcgtagg acagcaagga gtcagtgatg agcctaaata
     7141 tacaagggtc agtgtgggaa caacgattac taatgcttat aaatcccttg aaagccgtat
     7201 taataaatcg atttattttg gtcatacgaa gtccctatta tttggagaag aacttctaaa
     7261 agataaagtg cttttaaaac aaaccatgga cgcccttcaa agaaattctg attttgggaa
     7321 aagtattcct gttgttgctg tagaaggctc tgtggtagat gccttagaag ttttgccaga
     7381 tacccagcct atggtaggaa tatatattat ggagttattt gaaaataata aagaacggag
     7441 aagttttatt gaagaaaaag aattaggaaa tcttctgcaa gattttgaag aaaataaaac
     7501 tgggctgatt cccaggctga ttgtagggga aaaagactta aaacttgaag gagctgccat
     7561 tattaaagat tatgaattgc agggctggct ggatgaaaaa tatttaaaag gtattttatg
     7621 gcttttaaaa aaatcatacc gcttagaaat aagtgttcct tatcatgatt attatattgc
     7681 ctatgatgta tcagactcag aaactaaaat tgaattttca ggagatagtc aggacttatc
     7741 ctgtaaaatt tcgattgaga ctgaaggcag tattgatgaa tatattttgg atgaagaaat
     7801 gtttcaagca gaagaaataa gaaatctgga aaaggcagtt gaaaaagaaa ttgaaaaaga
     7861 agttcttgat tcaattgaaa ttctccaaaa aaaatataaa acagatgtca tagaaatagg
     7921 taaaagatta gaaagacaaa agccggagat ttggaaagaa ataaaagaca actgggatga
     7981 agtttttgaa aatatggact ttacagttga tgtaaaagtt tatgctcgtc gggttggaca
     8041 aacaaaataa gctcctttgt ataccaataa tagaaatggg aataataata acaaaataca
     8101 aagggggaaa taaaatgaaa aagataaatg ttattcattg gtttagtaaa gaaaaaacaa
     8161 ttcttttatt gtcattaata tgcggttttc ttataacagc ttttattctt catgcatcta
     8221 aggaatattc agaaactata caaagtggtt tagccgctaa tgtaatccgt tttcatgtta
     8281 ttgctaatag tgactcaaaa gaagatcagg aaataaagga aaaagtaagg gatgctattt
     8341 taaatgaaat ggctccagtt ctttcagatt ctgattcaat tgaagaaacc agaaatctaa
     8401 ttcttgataa tagagataaa ataaaaaaga tagcagaaga agttattcat aagaatggaa
     8461 aatcttatcc ggttgaagtg actctaggtc ctgctgtatt tcctactaaa cagtatgggg
     8521 atattgtact gcccacaggg gaatatgagg cacttagagt atctatcggc aaggcccagg
     8581 ggaaaaactg gtggtgtgta atgtttcctc ctctttgctt tgttgatgta acccatggtg
     8641 ttgtgcctga ggagtcaaaa acagatttaa gaaaagttct tacggatgaa gaatataaaa
     8701 tcattattac acaagtcaca aacgaggaag agattccagt taagataaaa tttaaaatcg
     8761 taaaatggtg gcaagagagg aagttaagta aaggacacca ttttgcccaa aaaagatttt
     8821 aaaaaatttg gaaatcgtat atatgtatgg aaagagtccg ctattaaggc ggattttttc
     8881 atgaataagt ttaaatataa taaagacaat ttatttattt tgacctgtct ttttaaaatt
     8941 ttttattaaa agtttttcta tatttttgtt cttttgttaa ttgaatcgga taaaaattta
     9001 tctctttaca aatactacta tcaacacaag tttgtgatgg gaggatttca atgaagataa
     9061 gtaaagttgc cacaggtctg ctgatttttt taatgatcgt tagtacaagc ctggctgttt
     9121 ttacttctta tataactcct gagtctgtgg aagcagctac ttatacctgg ggctctgaag
     9181 gggctactgt cagggaaata caaacaaagt taaaacaatg gggatactac aatggcaata
     9241 tagatggcaa atatggttat aatacctgga tggccgtaag aaagtttcaa gccaaaaacg
     9301 gattaaaggt ggatggaata gccggccctg aaattttaag aaaaataggt attaatgttc
     9361 catcaggagg cgggggcagt tcgggaaaag tatattcctg gggagccaga ggagaaaccg
     9421 taagagaaat tcaaagaaga ttaaaaaact ggggctatta taatggcagc gtggatggga
     9481 tttatggcta tggaacctgg tcggctgtaa aaaaatttca gcaaaaaaac ggtcttaagg
     9541 cagacggggt aacaggtgct gcaacccttc aagctctggg tattccaacg ggtcaaagtc
     9601 agacttcatg ggaaagaaac ctctatcttc tggcagcagc tattcatggg gaagccagag
     9661 gagaacctta tgaagggaaa gtcgcagtag gagcagttat tatgaaccgg gtaaggcata
     9721 gttccttccc caataccata gcaggagtta tttatcagcc gggagctttt gatgctgtgg
     9781 cagacggaca aataaatctg actccggatg acagtgcttt taaagcggcc agagatgctt
     9841 taaatggctg ggatccaaca ggaggcgcca tttattattg gaatcctgca acggcgacaa
     9901 gcagctgggt ttggtcaaga cctattatta aacaaattgg aaaacatgtc tttgcaaaat
     9961 aaaactttct atataaaaaa ttagacagtt tttatgtgct ttaaagaaca ggagggaaaa
    10021 gatgaaggct aaaaaaatta tactacttat ggttaactta atcatgtttt taaatttagc
    10081 ctattctttt tggatttata aagtaacacc aaaggctgta gacaccatca cttatacatg
    10141 gggttcagaa ggagacgtgg taaggcaaat ccagcaaaag ttaaaacagt ggggttatta
    10201 cgaaggtaat gtagatggta aatatggtta taaaacctgg gaagcggtaa aggcttttca
    10261 aagcaagaat ggactaaaag cagatggaat agcaggaaag cagactttag agaaaatggg
    10321 aattcaagtg aaggatagtt ccagaaaaca aggaggggat aacgtagcaa gtaataaaga
    10381 tcttatgctt atggcagcag ctattcacgg ggaaggaaga ggagaacctt acgaaggtca
    10441 ggtggcgata ggagccgtga tattaaaccg ggtaaggcat agttctttcc ccaataccat
    10501 agcaggggtc atttatcagc ccggggcttt tgatgccgtg gcagacggac aaattaactt
    10561 ggaaccagga gacagtgcaa tcaaagcagc cagggatgct ttaaatggct gggatccaac
    10621 aggaggtgcc atttattact ggaatcctgc aacggctaag agtaaatgga tttggtctgt
    10681 gcccattaca aagcggattg gcaaacatgt atttggcaaa aaataatgct taaggggtga
    10741 atactatgga agaaaaagat aatagattat cccagtggaa acgcttatcg ggcggcagtt
    10801 ttttaattgc tttggggttg ttgttaggct ttggtattta tcagtataag caaaagctta
    10861 attacaggca gtacttacaa aaccgttttc aggaatcctt ttatgaggca acgacttatg
    10921 tggacaatat ggaaaatctt tttacaaaag taagattaac aaaaagtccg gatcagagtg
    10981 cccctctgtt tgcccaaatt tggcgagaag cttcttcagc tcaggataat ttggcggaat
    11041 taccttatga ccataataca atttcaaaag ctttaaaatt tttatcccag gccagtgatt
    11101 tttccttttc tatgaccaat aaagccattg aaggttttga actggatgag gaagactgga
    11161 aaaaggtaga ccagttaaaa aaatatgcta aaatcctttc tgatgagtta aataaaatta
    11221 aacaagaggt taatgatggc aagcgtattg actgggaaga aatagaaagt gaaggagaaa
    11281 aaaggttagc caaaggaaat acaaaagcaa ttacaggttc cttttcagaa atcagcaagc
    11341 agtttgaaga tattcctact ttaatttatg acggaccttt ttcagatcat attgaaagaa
    11401 tggaacccag aatgaccaga aataaaaaga aaatttctaa agaagaaggg aaaaaggttg
    11461 tagaagaatt tattgggaaa gataaagtta aaagtattaa gcaaacagga gaaacggata
    11521 caaaagtgga acatacgatt cctgtatata gctatcaggt tgttttaaga gatcaggatg
    11581 agccgacgat ttcaatcgat gtgacacaac acggcggact ggtattatgg atgcttaatt
    11641 atactgaaaa agagtccacg gaagaaaagc tcagcttaga agaagccaga aagaaagcag
    11701 aaaaattttt agaagaaaga aattatccca atatgaaagc aagttattat gaaaaggtag
    11761 atgggaccgt agttattaat tttgcccctt atgaaaataa ggtgattatg tatcctgatt
    11821 taataaaggt taaggtggct atggatgatg gagaaatcat tggctttgag tccctgggtt
    11881 atattatgat gcatcatgac aggaaactgc cggaaattaa aattacagaa gaagaagcaa
    11941 gggataaaat tacgaaagat tttaaagtcc agcaagttaa tatggcagtt attcctttag
    12001 aatctaaaag agaggtttta tgctatgaat ttaagggcga atttgataat caactctttt
    12061 taatttatat taatgccgaa agcggaaaac aagaaaaaat ccttcaaatc ttgcagagta
    12121 aaaatgcagt attgactaaa taaggatgct tatggcatcc ttatttatat caggaaaaac
    12181 attatctttt gatattatct tgataaagca tagtttcaat aaggtttgca aacaaaatag
    12241 agacaagctt tatgataaaa atataattat atccttgcaa ccattcgtta tgattgcaag
    12301 agaaagattt agttcagaag gaaggttgag ggaggtatcc ggggaagggg cactttatgg
    12361 catttgaaaa cggattattt agctttttgc ttataatgat tatatcattt attttaacag
    12421 tcataggcat tatacttctt gtaatattta agaataaaac taatggctta aggtttattg
    12481 gctttttatt aatctcaata gcgatgataa tgatattatt ttcaaaaaag ctttgtcttg
    12541 atacagtcag ggtaaaagaa acagtggatg ttgcaccaat gacagaactc aaattttctt
    12601 caatattaaa agtgttaaag agattatgga aagcattgaa tcatttgacg aattggaagt
    12661 gaatacgatt gatggcgtaa atattgcaat gccagtaaga acaactgatg gaattgttga
    12721 aatgttaact ggggttgacc caaaaggagc ttatttaatg gttaatatat atgtatatgg
    12781 tagtgtgaaa ggtgccaatg aatcatatga acacaactac aaaaatcttg gaaaagatta
    12841 tggaattatg actagtggtg gaagcaatag tgacaggtat tttgttacat ataaaaatca
    12901 gttgcgttta agtggagacg ctctgtattt gctaacagac caatatgtta catatgttta
    12961 ttttcagaag cgaaacatga ttatcaaatt aatggaaaca aaaagggacg atacggacag
    13021 caaaataaat gactatataa aattgctctc agataagtta tcagaattgg aatagattga
    13081 gcatgcattt tttatgaaaa ttatttgtct gaaacaattt aaggggctgg aatttgccag
    13141 cccattttta atggtttctc ttgtatttat aaaatgactt gaattcatac atataaaaca
    13201 ttaaaatggt atggaaa
//
LOCUS       DAOSUD010000058        13185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2798_length_13185_cov_25.509444, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000058 DAOSUD010000000
VERSION     DAOSUD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_13185_cov_25.509444"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1514
                     /gene="glmU"
                     /locus_tag="PLG49_07945"
     CDS             147..1514
                     /gene="glmU"
                     /locus_tag="PLG49_07945"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOQ17286.1"
                     /translation="MNQLSAIILAAGEGTRMKSKMPKVLHTVLGRELLNHVIDSAKSA
                     GTERICVVIGHKGDEVKEKIGDCVEYVEQKERLGTGHAVMQADGFIQEEGDVLVLYGD
                     TPLITGETLKRLIDTHRKENNGVTILSAIVENPAGYGRIIRDEKGNFLKNVEHKDASE
                     EELKVNEINGGMYCFKAKLLKEALKELTNNNSQKEYYLTDTLEIILSKGHRVNALVVE
                     DSDEILGVNSRVQLAQVTQIMKRRINKMHMEAGVTIIDPENTYIEPEVKIGKDTIIYP
                     GCNLQGKTVIGEDCIIGPNSTIKSSQVADKVTVQNSVVLESIINSNTTVGPFAYLRPN
                     SNIGSHVRIGDFVEIKNSTIGNHTKVSHLTYIGDADVGERVNFGCGTVVVNYDGISKH
                     RTKIGNDAFIGCNTNLISPVTVHDEAYTAAGSTITEDVPAKALAIARARQINKNDWVE
                     KRKKK"
     gene            1611..2564
                     /locus_tag="PLG49_07950"
     CDS             1611..2564
                     /locus_tag="PLG49_07950"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOQ17287.1"
                     /translation="MSTSNSEIKIFSCNAHKPLAEEIAQVLGLKLGNSEVGRFSDGEI
                     SVKIDEKVRGADVFVIQPTSTPVNEHLMELLIMIDGLRRASAGRITAVIPYYGYARQD
                     RKTRARDPISARLVADLIQTAGADRVLTMDLHCAQIQGFFTIPLDHLVGLPLLTKYYL
                     EKFESLEDFVAVSPDLGSVTRTRAFATKLEIPLAIIDKRRPKANESEVMNIIGNIEGK
                     NVILVDDMIDTAGTISNAADALIERGAKRVYACCTHPVLSGPAIERIEKSAISELVVL
                     NTIYLPEEKRIPKIKEISVAPIVAEAIHRIHEDLSVSRLFD"
     gene            2664..3236
                     /gene="pth"
                     /locus_tag="PLG49_07955"
     CDS             2664..3236
                     /gene="pth"
                     /locus_tag="PLG49_07955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOQ17288.1"
                     /translation="MYAVIGLGNPGLQYAATRHNVGFEVIERLAYENGIKVEKRKHQA
                     LIGEGIIGGEKVILAKPQTYMNLSSQSVIEIMNWYKLDNKHIIIIYDDISLPIGFIRV
                     RTKGSDGGHNGIKNIISCLNSQEFLRVRVGVGEKPPGWDLADYVLSRFNREEIEEIVQ
                     SIKIASDAVETIIKEGPQKAMNQYNQKVRE"
     gene            3256..6774
                     /gene="mfd"
                     /locus_tag="PLG49_07960"
     CDS             3256..6774
                     /gene="mfd"
                     /locus_tag="PLG49_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOQ17289.1"
                     /translation="MNGILQPLLELDAYQKLLEDLKNHNTPLLATGMVDSQKAHLVAA
                     LKNHSLRPAVIITMDDLKAKELFEDLQFFIKENLYFYPSKDILFYSADVHSMDIVKQR
                     VNVLKALAKEENTIVVLSIEALLDRLVKKDLFKSFILNLKVGSIIDIKDLTYQLFLMG
                     YEHSDLVESPGQFALRGGIVDVYPSTEESPYRIEFWDDEVDSIRLMDPVSQRSVEKVD
                     SIEIFPMRELVFTDEAIKRGISYIEEETRKTIKKYEKKNLKEEKETLQKSINTILEKL
                     KNQKGFAGIEAYVNYFFEETVTLANYFLKDTIVFLDEPQRIKEKCHKVISEFEDSMKR
                     RLEKGYILPRQCETIFRYEDILNSIENFDIVLLTMLTRTAGDFLLKELIQFPVKSVES
                     FYKQMDLFEKNLLYWKKKKFRILILAGPKSKGERLAKELNDRNIFCTFRDNLSEPVPK
                     GEIIVSQGSLYKGFEYGLIDFIVISDKEVFGELKKKRKARKKISGSKIENFTDLNPGD
                     YVVHENHGIGIYQGIEKITVDNVSKDYLKIRYLDNGNLYVPINQMDMIQKYIGGEGRA
                     PKLNKLGGNEWVKAKAKVRKAVEDLAKELIELYAKRQSSKGYKYGPDTVWQKEFEDMF
                     PYEETEDQLIAIEEVKRDLESDKIMDRLICGDVGYGKTEVAIRAAFKVVQEGKQVAYL
                     VPTTILAQQHYNTFLQRMKDFPIEVGLLSRFRSKSEQTATIEGLKKGMVDIVIGTHRL
                     LSKDVAFKDLGLLIIDEEQRFGVVHKEKLKQLKEKVDVLTLTATPIPRTLHMSLIGIR
                     DMSILNEPPEERYPVQTYVMEYNTEFVKDAIRRELARGGQVYYLYNRVQNIDKVTAKI
                     QELVPEAVVAYAHGQMSERELENIMMDFINKEIDVLVCTTIIESGLDISNVNTIIIQD
                     ADTLGLSQLYQLRGRVGRSNRLAYAYLMYQKDKVLQEGAEKRLQAIKEFTEFGSGFKI
                     AMRDLEIRGAGNLLGAQQHGHIDAVGYDLYSKLLEEAIRKEKGEEIKEHFETAIELDI
                     DAYIPQNYIEDELQKLEIYKKIASIQGERDYYDIQEEIEDRYGDLPESVQNLLEIALI
                     KWEAHELKITSISQKKLIITITFKEDAPIDAAKIPDLLKEYRNSLSFAADKNPYFTYR
                     LQNDKDIEVIRQIKNILQSLKCLKLE"
     gene            6835..7836
                     /locus_tag="PLG49_07965"
     CDS             6835..7836
                     /locus_tag="PLG49_07965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ17290.1"
                     /translation="MKKLIPIFVLSTLVCFLLSACNSDYLSKKEYIAVINNEKIKVDE
                     YKVYLWRIKQLYQNIGQEDIWETKFDGKPAEEVAKERAFDSIKSIKIQVQEGRKMGLT
                     FAEEEKEDILSQTEELMNAIGEEEMKNMGLTEDTIRTVVEDSVMSQKVYTEMTKDFVG
                     NKADFELFFETNKEKLKQVRVKHILLKTHDLVDGKLVALPQEEQIKAKERAEEALGRA
                     RNGADFDLLVQEYSQDTDSLPNNGEYIFMRGQGIDQNFEEAAFSLKVNEISDIVETVY
                     GYHIIKLEEIIEPDKDFIEQEYYDLMKKNYFNKKLEEWINNSTIEINQEVWEEIKIK"
     gene            8039..8590
                     /gene="spoVT"
                     /locus_tag="PLG49_07970"
     CDS             8039..8590
                     /gene="spoVT"
                     /locus_tag="PLG49_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOQ17291.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDHDGQV
                     ILKKYSPIGELGNFAKEYAEALAQTTGLITCITDKDQIIAVSGGSKKELLDKNISKSL
                     EKVIEERSVFTANKDEDKFIPITADENTGNFYSELIYPIISEGDVVGSVIFLSNEKNV
                     VMGEVEKKLAQSASGFLGKQMEQ"
     gene            8725..9525
                     /gene="mazG"
                     /locus_tag="PLG49_07975"
     CDS             8725..9525
                     /gene="mazG"
                     /locus_tag="PLG49_07975"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOQ17292.1"
                     /translation="MKKKEKYTYDDLLEIMRILRSENGCPWDRKQTHESILPNLLEET
                     FEVIDAVKKNNKNALKEELGDLLLQIVFHARMAEEDNLFSMEEVIDGICKKLILRHPH
                     IFGDVIVHTAEEVLENWEQIKKVEKGYHTQTEVLKSVPEVFPALMRAYKVQKKAAEIG
                     FDWDNIEDVIKKVEEEKNELLEALKEGNNSKIVEEYGDFLFSIVNFARFLKINPEFAL
                     TNTTEKFINKFEYVENTAISQGKKLEEMTLKEMDELWEESKRKEIEKS"
     gene            9593..9871
                     /locus_tag="PLG49_07980"
     CDS             9593..9871
                     /locus_tag="PLG49_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ17293.1"
                     /translation="MNKSELVAAMAAKAELSKKDTEKVLKAFEEVVTEELAKGGKVQL
                     VGFGTFDVAERAAREGRNPQTGKPMKIPASKAPRFKAGKVLKDAVNKK"
     gene            9987..10226
                     /locus_tag="PLG49_07985"
     CDS             9987..10226
                     /locus_tag="PLG49_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOQ17294.1"
                     /translation="MRLDKYLKISRLIKRRTIANEACDAGKVLLNGKAAKAGAQVKAG
                     DIIEIRFGDNVTKVEVLEVKEHVKKEEINNMYRYL"
     gene            10336..10623
                     /gene="yabP"
                     /locus_tag="PLG49_07990"
     CDS             10336..10623
                     /gene="yabP"
                     /locus_tag="PLG49_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOQ17295.1"
                     /translation="MEEKAVNTKGHSIFIKERQSMMITGVIDVLSFDESNVDIETEMG
                     MLAIKGTDLHVNKLNLEKKELELEGEIESLIYHDGDSVGKGGTSLLGRLFK"
     gene            10636..11157
                     /gene="yabQ"
                     /locus_tag="PLG49_07995"
     CDS             10636..11157
                     /gene="yabQ"
                     /locus_tag="PLG49_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOQ17296.1"
                     /translation="MIVSVSEQANTFLIAVLSGIVIGIFYDIFRIIRKIIPHPNWLVQ
                     LEDLIYWIIVSAFMFLVLFTNNYGEIRGFALLGAFLGNILYFFTLSIIVMKFSDWIIY
                     WLKKIVTALIKMILIPLKLVLRIFYYPLKWISYPVKRFTHKSRFFFNRVQNKLKRKIR
                     QIFKELYIMCRKV"
     gene            11269..11577
                     /locus_tag="PLG49_08000"
     CDS             11269..11577
                     /locus_tag="PLG49_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ17297.1"
                     /translation="MKKRRRKKKFFQLGWNTILLSIFFVMIAFKTYDLQKINRELQEQ
                     KENYIKKIEEETKENAKLLEQKEYSESDLYIEKIAREKLGLVKEDEIVFMEKSEESID
                     "
     gene            11714..>13185
                     /locus_tag="PLG49_08005"
     CDS             11714..>13185
                     /locus_tag="PLG49_08005"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17298.1"
                     /translation="MARIEYNTFENLEYCERKEGMQLKDILLNISFKDLSIDIVAFMV
                     SRVVLFTNLLPLSIAYFASSLSSKANRIWLLLFSTVGIISAKQDVSVIKYLIIFILIL
                     LMVSYLEKKGYKQTKIGQAIIAMVSTFLAGIFFLVLKNFAGYYLVVAILESIFVFTTT
                     YIYSSGIFSLRGHLKRTVLSTEEIISLILLFGTSIAGLIDFSINGVYFREVISMMIIL
                     IFGFVGGPSLGAAIGLIVGLILTLIGVTPAIFIGIFGLSGMVASLFKDIGRLGTSMGF
                     LLSYIIFDFYFQGELLDFIFIKPVIVAIVLFITLPKSCILYIKSFVSFEPEMGQELYY
                     KRIRDITAQQLKSFSDAFFKLSKTFSYLSKKKKNLNQKDVSRLIDDVASKVCSDCGLC
                     LHCWESDFYNTYQTVFSILSAAERKPQIDLKDIPREFLDKCIKAEEFIDTTNRMFELY
                     KLNLLWHNRIVESRELVCEQLKGVSTIIDSLATEIYGKIY"
BASE COUNT         4829 a         1627 c         2729 g         4000 t
ORIGIN      
        1 agttttattt tagcaagtgt gtatttttta cacacttttc ttttgtgtat aaagattttt
       61 tgttgcaata tttttttaga aatgagagaa taaaaaagga catgatattt tgaataggaa
      121 gaggtgtata catgacgaat tcatctataa atcaattatc tgcaattatt ttggctgctg
      181 gtgaaggaac cagaatgaaa tcaaaaatgc ctaaagtttt gcatacagtg ttaggcaggg
      241 aattgttaaa tcatgttatt gattcagcaa aatctgcagg aactgagaga atttgtgttg
      301 ttattgggca taaaggagat gaagtaaagg aaaagatagg agattgcgtt gaatacgttg
      361 aacaaaaaga gcgtttaggg acaggtcatg cagtcatgca agccgatggt tttattcagg
      421 aagaaggtga tgttttagta ttatatggag acacaccgct aattactggc gaaacattaa
      481 aacgtttaat cgatacccat agaaaagaaa ataatggagt aactatttta tctgccattg
      541 tagaaaatcc tgctggctat ggaagaatca taagggatga aaagggtaat tttttaaaga
      601 atgtagagca taaagatgcc agtgaggaag aattaaaagt caatgaaatt aatggaggaa
      661 tgtactgttt taaagctaaa ttgttaaagg aagctttaaa agaacttacc aataataata
      721 gtcaaaagga atactatttg acggatacgc ttgaaatcat attatctaaa ggtcataggg
      781 tcaatgcttt agttgtggaa gacagcgatg aaatattagg tgttaattca agagttcaat
      841 tggcacaggt aacgcaaatt atgaaaaggc gaattaataa aatgcatatg gaagccggtg
      901 tgactattat agatccggaa aacacttata ttgagcctga agtaaagatc ggaaaggata
      961 cgattattta tccgggctgc aatttacagg gaaagactgt tataggagaa gattgtatta
     1021 taggtcctaa ttcaaccatt aaaagttccc aagttgctga taaagttacc gtacaaaaca
     1081 gtgtggtgtt agaaagcatc attaattcca atacgaccgt ggggcccttt gcttatctta
     1141 gacccaatag taatattgga agccatgtaa gaattgggga ctttgtagaa ataaaaaatt
     1201 ctaccatagg aaatcacaca aaggtatctc atttaaccta tatcggagat gcagatgtag
     1261 gtgaaagagt taattttgga tgtggtacgg ttgttgttaa ctatgacggt atcagtaagc
     1321 acagaacaaa aataggaaac gatgccttta ttggttgtaa tacaaattta atatctccag
     1381 ttacagtaca tgatgaagct tatacagcgg caggatctac tattactgaa gatgtccctg
     1441 caaaagcttt agccattgca agagcgagac aaattaacaa aaatgactgg gtagagaaaa
     1501 gaaagaaaaa ataagcagca taataatttt atttttttat aaagtatgtt ataattgtgg
     1561 cggcaattag ggggcgaaag aggaagatat tttaaggagg attttttaaa atgagtacta
     1621 gcaacagtga aattaaaatt ttttcttgta atgcacacaa acctttggcg gaagagatag
     1681 cacaggtatt aggattaaag ctgggcaatt cagaagttgg caggtttagc gatggtgaaa
     1741 tatctgtaaa aattgatgaa aaagtaaggg gagcagatgt atttgtcatt cagcctactt
     1801 ctacaccagt caatgaacat ttaatggaat tgttgattat gattgatggt ttaagacgag
     1861 cttcggcagg aagaattaca gcggttattc cttactatgg ttatgcaaga caagacagaa
     1921 aaacaagagc cagggatcca attagtgcga gattggttgc agatttaatt cagactgcag
     1981 gagcagacag ggttttaaca atggatctcc attgcgctca aattcaggga tttttcacaa
     2041 ttcctctgga tcatttagta ggacttcctt tgctgaccaa atattattta gaaaaatttg
     2101 agtcactgga agactttgtt gcagtttctc cagacctggg aagcgttacc agaacaaggg
     2161 cctttgctac aaaattagaa ataccgttag ctattattga caagagaaga cctaaggcaa
     2221 atgaatcaga agtgatgaat attattggca atattgaagg aaaaaatgtt atattggttg
     2281 atgatatgat agatactgca ggaaccattt ctaatgctgc cgatgcttta attgaacgtg
     2341 gagcaaaaag ggtttatgct tgttgtactc atcctgtact ttcagggcct gctatcgaac
     2401 gaattgaaaa atctgctatt tctgagttag tcgtattaaa tacaatatat ctgccagagg
     2461 aaaaaagaat tccaaagatc aaagaaattt ctgttgcacc tatagtggca gaagctattc
     2521 atagaattca tgaagattta tctgtttcca gattgtttga ttaattgata gccattagga
     2581 gagactttaa taataagtgg ctgtttaaat tataagccct agttctacta gggctatttg
     2641 ctatttttga tggaggtgta gcaatgtatg cagtgattgg cttaggcaat ccaggccttc
     2701 aatatgctgc tacaaggcat aatgttggct ttgaagtgat tgaaagactg gcttatgaaa
     2761 atggtattaa agtagaaaaa agaaagcatc aggctttaat tggggaagga atcattggag
     2821 gagaaaaagt tatattggct aagccccaaa cctacatgaa tttaagtagt caaagtgtga
     2881 tagaaatcat gaattggtat aagttagata acaagcatat aattataata tacgatgata
     2941 taagtcttcc cataggtttt atccgtgtac ggactaaagg aagtgacgga gggcataatg
     3001 gaataaaaaa cattatatca tgcttaaatt ctcaggaatt tttaagagta agggtcggcg
     3061 taggagaaaa acctcctgga tgggatttgg ctgactatgt tttgagccgt tttaatcgtg
     3121 aagaaatcga agaaattgtc caaagtatta aaatagcttc agatgctgtt gaaacaatta
     3181 ttaaagaagg tccacaaaag gcaatgaatc aatataatca aaaagtaagg gagtgatggt
     3241 ttggagtctt tggatatgaa cggaatcttg cagcctcttt tagagttaga tgcctatcaa
     3301 aaacttttag aagacttaaa gaatcataat actcccctac tggcaacggg gatggtagat
     3361 tctcaaaaag cacatcttgt tgctgcttta aaaaatcata gtttaaggcc tgctgtcatt
     3421 attacaatgg atgatttaaa ggctaaagag ctatttgaag atctccaatt ttttataaaa
     3481 gaaaatcttt atttttatcc ttcaaaagat attttatttt atagtgcaga cgttcacagc
     3541 atggatattg taaagcaaag agtcaatgtc ctgaaggctt tggcaaaaga agaaaacacc
     3601 atagtcgttt tatcgataga agccttattg gacagacttg taaaaaaaga tttgtttaaa
     3661 tccttcatat taaatcttaa agttggaagc atcattgata taaaggattt aacttatcag
     3721 ttgtttttaa tgggctatga gcattcagat ttggtcgaga gcccaggaca atttgctctc
     3781 aggggtggca ttgttgatgt ctatccgagt actgaagaaa gtccttacag gattgaattt
     3841 tgggatgatg aagtagattc tatccgatta atggaccccg tttctcaaag atctgttgaa
     3901 aaagtcgatt cgattgaaat atttcccatg agggagcttg tttttacaga tgaagcgata
     3961 aagagaggca tatcctatat agaagaagaa acaagaaaaa caataaaaaa atatgagaaa
     4021 aaaaatttga aagaagaaaa agaaactcta caaaaatcaa ttaatacaat tttagaaaaa
     4081 ctaaaaaatc aaaaaggttt tgcaggaata gaagcttatg tgaattattt ctttgaagag
     4141 acggtgacct tagcgaatta ttttttaaaa gacaccattg tttttttaga tgagcctcaa
     4201 agaataaaag aaaaatgtca taaggtaatc agtgaatttg aggacagcat gaagagaaga
     4261 ctagaaaagg gatatatcct cccccggcaa tgcgaaacaa tttttagata tgaagacata
     4321 ctaaattcca tagaaaactt tgacattgtc ctcttaacga tgttgacccg cactgcagga
     4381 gactttcttc ttaaggaatt aattcagttt cctgtaaaat cggtagaatc tttttataag
     4441 caaatggatt tattcgaaaa aaatcttttg tattggaaaa agaaaaaatt caggatactt
     4501 atattggctg gtccaaagag taaaggagaa cggctggcaa aagaattaaa cgacaggaat
     4561 atattttgta ctttcagaga caatttgtct gagcctgttc caaaaggaga gattattgtt
     4621 tcccagggca gcctttataa gggttttgaa tatgggctga ttgattttat tgttatttct
     4681 gataaagaag tctttggaga attaaaaaag aaaaggaaag caagaaagaa aatcagtgga
     4741 agtaaaatcg aaaactttac agatcttaat cccggagatt acgttgtaca tgaaaatcat
     4801 gggataggga tttatcaagg cattgaaaag attacagtag ataatgtcag taaagattat
     4861 ctgaaaatcc gttacttaga caatggaaac ttgtatgttc cgattaacca aatggatatg
     4921 attcaaaagt atataggagg tgaaggcagg gctccaaagc ttaataaact tgggggaaat
     4981 gaatgggtaa aagccaaggc aaaggtaaga aaagcagttg aagatttagc gaaagagctg
     5041 atagagcttt atgcaaaaag acaaagttcc aaaggttata aatatggtcc ggatacggtt
     5101 tggcaaaaag aatttgaaga tatgtttcct tatgaagaga ctgaagatca gttaattgcc
     5161 attgaagaag tcaaaaggga tttagaaagt gacaaaataa tggaccgttt aatttgcggg
     5221 gatgtaggct atgggaaaac agaagtagcc ataagagctg ctttcaaagt cgttcaggag
     5281 ggcaaacagg tagcttatct ggttcctaca accatcctgg cccagcagca ttataatact
     5341 tttctccaaa gaatgaaaga ttttccgatt gaagtgggct tattatcacg attcagaagc
     5401 aaaagtgagc aaacagcaac gattgaagga ttaaaaaaag gtatggtaga tattgtgata
     5461 ggtacccaca ggcttttatc caaagatgtt gcctttaaag atttaggctt gctgataata
     5521 gatgaagagc aaagatttgg tgttgttcat aaagaaaagc taaaacagtt aaaagaaaaa
     5581 gtggacgttt taacattaac cgctactccg attccccgga ctcttcatat gagtcttata
     5641 ggtatcaggg atatgagtat attaaatgaa ccacctgaag aaagatatcc ggtacaaact
     5701 tatgtcatgg aatataatac agagtttgta aaagacgcca tccgcagaga actggcaaga
     5761 ggtggacaag tatattattt atacaacagg gttcaaaata ttgataaagt aacagcaaaa
     5821 attcaggaac tggttcctga agcggttgtt gcctatgctc acgggcaaat gagcgaaaga
     5881 gaattagaaa atataatgat ggattttata aataaggaaa ttgatgtctt ggtgtgtacg
     5941 accattatcg aaagtggtct cgatatttcc aatgtaaata caattattat tcaggatgct
     6001 gatactttag gtctgtctca gctttatcag ttaagaggaa gagtcggacg ctctaaccgg
     6061 ctagcttatg cttacttgat gtatcaaaag gacaaagtcc tgcaggaagg agcagaaaaa
     6121 agactgcagg ctatcaagga atttactgag tttggttcag gctttaagat tgctatgagg
     6181 gacctggaga taaggggtgc agggaactta ttaggagccc aacagcatgg ccatattgat
     6241 gcagtaggct atgatttgta cagtaaattg ttggaagaag ccatcaggaa agaaaaagga
     6301 gaagaaatta aagaacattt tgaaacggcc atagagttag atattgatgc ttatattcca
     6361 caaaattata tcgaagatga gcttcaaaaa ctggaaattt ataaaaagat tgcaagcata
     6421 caaggagaaa gagattatta tgacatacag gaagaaattg aagacaggta tggggattta
     6481 ccggaaagtg ttcaaaattt attagagatt gcccttataa agtgggaggc tcatgaattg
     6541 aaaattacat ctatatcgca aaaaaagctt attattacaa ttacatttaa agaagatgcc
     6601 ccaattgatg cagcaaaaat accggatttg cttaaagaat accgcaatag cctgagtttt
     6661 gccgcagata aaaaccctta tttcacatat cgtttgcaaa atgataaaga tattgaagtt
     6721 atcagacaaa ttaagaatat attacaaagt ttgaaatgtt tgaagttaga atagaaatta
     6781 cttataatat tattagggag aaatgttatt tcttagagag atggggttga agtgatgaaa
     6841 aaacttatac caatattcgt cctgtcaaca cttgtatgct ttttgctttc tgcttgtaat
     6901 tctgattatt taagtaaaaa agaatatata gcggtcataa acaatgagaa aattaaggtt
     6961 gatgaatata aggtttattt atggagaatt aaacaattat atcaaaatat aggacaagaa
     7021 gatatatggg aaacaaagtt tgacgggaag ccagcagaag aagtggctaa agaaagagct
     7081 ttcgattcca ttaaatccat aaagattcag gttcaggaag gaagaaaaat gggacttacc
     7141 tttgctgaag aagagaaaga agatatttta tcccagacag aagagttaat gaatgcaatc
     7201 ggagaagaag aaatgaaaaa tatgggcttg acggaagata ctatacggac tgttgtagaa
     7261 gattctgtca tgagtcaaaa agtttatacg gaaatgacta aggattttgt aggaaataaa
     7321 gcagattttg aacttttttt tgaaacaaac aaagaaaagt taaaacaggt aagagtcaaa
     7381 catattttat taaaaacgca tgatctggtg gacggcaagc tggttgcctt gccacaggaa
     7441 gaacaaataa aggcaaaaga acgggctgaa gaagctctgg gaagggcaag aaacggagca
     7501 gattttgatc ttttggttca ggaatattct caagatacgg attctctgcc caataatgga
     7561 gaatatatct ttatgagagg gcaaggtata gatcagaact ttgaagaagc agcttttagc
     7621 ctgaaagtga atgaaatcag cgatattgtt gagacggtat acggatatca tattattaag
     7681 cttgaagaaa ttatagagcc ggataaggat ttcatagagc aagaatatta tgatttgatg
     7741 aaaaagaatt attttaataa aaaacttgaa gaatggatta ataattcaac catagaaatc
     7801 aatcaggaag tttgggaaga aattaaaata aaataatcga ttgaatgata atacttccgg
     7861 gcgtttgaat agctcgggag tattttttta taaaatttat aaataaaaga gtttttgata
     7921 tgaaattaat tatttttaaa tagtgaatta aaatggaaaa catgaataaa atagcaatat
     7981 taaaatcata ctaatagtga aaaccatcac gaaagctaga aattaaggag gaataaaatt
     8041 gaaggcgaca ggaatcgtaa gaagaataga tgatttaggc agagtagtaa ttccaaaaga
     8101 aatacgaagg actttaagaa tcagagaagg ggacccttta gaaattttta cagatcatga
     8161 tggacaagtt attttaaaaa aatactctcc tattggggaa cttggcaatt ttgcaaaaga
     8221 atatgctgaa gctttggctc aaacgacagg ccttattaca tgtattacgg ataaagatca
     8281 aattattgcg gtttcgggag gttctaaaaa agaattatta gataagaata ttagtaagtc
     8341 tttagaaaaa gtgattgaag aaagaagtgt ttttacagcg aacaaagatg aagataaatt
     8401 tataccaata accgctgatg agaacaccgg aaatttttat tcagaactca tttatccaat
     8461 tatttcagaa ggagatgtag ttggctcagt catctttttg tccaatgaaa agaatgttgt
     8521 gatgggagaa gttgaaaaga aattggctca atctgcttca ggatttttag gaaaacaaat
     8581 ggaacaataa tattgcagat agatttctat ctgcatttat caactttttt gttttttaat
     8641 tcaatttgac tatatatatt tttttcgatt tatgctatac tatataaaaa attaaatcgc
     8701 agcaatgatt gagttggtgt acttatgaag aaaaaagaaa aatatactta cgatgaccta
     8761 ttggagatca tgagaatttt aagaagtgaa aacggatgtc cctgggatag aaagcagacc
     8821 catgaaagta ttcttcctaa tttgctcgaa gaaacttttg aagtcattga tgcagttaag
     8881 aaaaataata agaatgcttt aaaagaagag ctgggggatt tattgcttca aatagttttt
     8941 catgccagaa tggctgaaga agacaattta ttttcaatgg aagaggtcat tgatggaatt
     9001 tgtaaaaagc ttatattaag gcatcctcat atttttggag atgttattgt ccatacggca
     9061 gaagaagttt tagaaaactg ggagcaaatt aaaaaagttg aaaaaggtta tcatacgcaa
     9121 accgaagttt taaaaagtgt accagaggtt tttcctgctt taatgagagc ctataaagta
     9181 caaaaaaaag cagccgaaat aggttttgac tgggataaca tagaggatgt tattaaaaaa
     9241 gtggaagaag agaaaaatga gcttctagaa gctttaaaag aaggcaataa cagtaagatt
     9301 gtggaagaat atggagattt tctcttttcg attgtaaatt ttgcaagatt tttaaaaata
     9361 aatcctgaat ttgccttgac aaatacgaca gaaaagttta taaataaatt tgaatacgtt
     9421 gaaaatactg cgatttctca aggtaaaaaa ttagaggaaa tgacccttaa agaaatggac
     9481 gagctttggg aagaatccaa gcgaaaagaa attgaaaaat cgtagtattt tactcgtaga
     9541 ttttaaaagg cttttatttt taattttaaa atgaaatatg aggaggaaga atatgaacaa
     9601 atccgaatta gtagcggcta tggctgcaaa agctgaatta agcaaaaagg acactgaaaa
     9661 ggtacttaaa gctttcgaag aagtagtgac tgaagaatta gctaaaggcg gaaaagttca
     9721 actagttgga tttggtactt ttgacgtagc agaaagagca gcaagagaag gaagaaatcc
     9781 tcaaacagga aaaccaatga aaattcctgc ttctaaagct ccaagattca aagcaggaaa
     9841 agtattaaaa gatgcagtta ataaaaaata agtagcatac atatttaaaa taaaaatcta
     9901 cacttattat gttattataa gtgtagattt ttttgtggta taatattttt gataagcttt
     9961 ttaaccgatc tacaggagga catgctatga gattagataa atatttaaaa ataagccgtt
    10021 tgattaaaag aagaaccatt gctaacgagg cttgtgacgc aggaaaagtt ttacttaatg
    10081 gaaaggcagc taaggcagga gctcaggtaa aagcaggaga tattattgaa atcagatttg
    10141 gcgataatgt aaccaaagta gaagtcttag aggtaaagga acatgtaaaa aaagaagaga
    10201 ttaataatat gtatcggtat ttatagcttt tataagcata tttcattcaa cgcccaatat
    10261 atatatatat aggaagctga caaatatatc agaagaacag tatttcatat atattgaata
    10321 aaaaggtggg gttgtatgga agaaaaagct gtaaatacta aaggacatag tatctttata
    10381 aaagaaagac agagtatgat gattacaggt gtaatagatg ttttatcttt tgatgaatct
    10441 aatgtagata tagaaacaga aatgggtatg cttgcaatca agggtacgga tcttcacgtt
    10501 aacaagctga atttagaaaa aaaggaatta gaactggaag gtgagataga aagcctgatt
    10561 tatcatgatg gagattctgt tgggaaaggt ggaacatcat tattgggcag gctctttaaa
    10621 taggggttga ttgccttgat tgtatctgtc agtgagcaag caaatacttt tttaattgct
    10681 gtattatcag gaattgttat aggaatcttt tatgatattt ttagaattat aaggaaaatc
    10741 attccacatc ccaattggtt agtccaatta gaagatttaa tttattggat tattgtttct
    10801 gcttttatgt ttttggtctt gtttacgaac aattatggag agattagagg ctttgcctta
    10861 ttaggagcat ttcttggtaa tatcctctat ttctttactc taagtattat cgtcatgaag
    10921 ttttcagatt ggattattta ttggcttaaa aaaattgtga ctgcacttat taaaatgatt
    10981 cttatacctt taaaactagt attaagaatt ttttattacc ctttaaagtg gatttcttat
    11041 cctgttaagc gtttcactca taaatccaga ttctttttta accgcgttca aaataaatta
    11101 aaaagaaaaa ttagacagat ttttaaagag ctatatatta tgtgcaggaa agtataaaaa
    11161 gtataaaata aaaaaggaga ttctttttaa atgaagaata tttaatttta acaggttttt
    11221 ttatggaata atcgttgctg gtagtttgtt tttaaggtgg ggataagggt gaaaaagaga
    11281 cgaaggaaga aaaaattttt tcaattaggc tggaatacga tattattgtc tatatttttt
    11341 gtcatgattg cttttaaaac ctatgatttg caaaaaataa acagagaatt gcaagaacaa
    11401 aaagagaact atattaaaaa gattgaagaa gaaacaaagg agaatgccaa acttttagaa
    11461 caaaaagaat atagtgaatc ggatttatat attgaaaaga ttgcaaggga aaaattaggt
    11521 ctggtaaaag aagacgaaat tgtatttatg gaaaaaagtg aagagagtat agattgagga
    11581 cgcttgggcg tcctttattt tttgtaaaat tgctgttata aatgtctaaa ggtttttttg
    11641 acgacttcca tataatgaca aataattttt gccttcttta ttataatata ggcagaaatt
    11701 aggagggtgt tatatggcga gaattgaata taatacgttt gaaaatctgg agtattgtga
    11761 aagaaaagaa ggaatgcaat taaaagatat attattgaat atatctttta aagatttatc
    11821 catcgatatt gtagctttta tggtttccag ggtagtgctt tttaccaatt tactgccttt
    11881 aagtattgca tattttgcat cttctctatc ttcaaaagca aacagaatct ggcttttgtt
    11941 attttcaaca gtaggaatta tcagtgcaaa gcaagatgtt tcagttataa aatatttaat
    12001 catttttatt ttaatcctgc taatggtcag ctatcttgag aaaaaaggat ataaacagac
    12061 taaaattgga caagctatta ttgcgatggt gagtacattt ttagcaggga tctttttttt
    12121 agttcttaaa aattttgcag gatactattt agtagtagct attttagaaa gtatttttgt
    12181 ttttacaaca acttatattt atagcagtgg catattttct ttaagaggtc acttaaaaag
    12241 gacagtatta agcacggagg aaattattag tttaattttg ctttttggta cttctatagc
    12301 aggcttaatt gatttttcca taaacggggt ttatttcagg gaagtcatca gtatgatgat
    12361 tattttaatc tttggatttg taggagggcc ttctttaggt gcagctattg ggcttattgt
    12421 tgggctgata ttaactttaa taggcgtaac acctgcaatt tttataggga tatttgggct
    12481 tagcggtatg gtggccagct tgtttaagga cataggacgt ttaggaacga gtatgggttt
    12541 tttattgtct tatattattt ttgattttta ttttcaaggg gagttgttag attttatatt
    12601 tattaagcct gtcatagttg ctattgtact atttattaca ttacctaaaa gttgtatttt
    12661 atatattaaa agctttgtct cttttgagcc tgaaatggga caagagttat attacaaaag
    12721 gataagggat attacggcac agcagcttaa aagcttttcc gatgcttttt tcaagttatc
    12781 aaagactttt agctatcttt caaagaaaaa gaaaaactta aatcaaaaag atgtttcccg
    12841 tcttattgat gatgttgctt ctaaggtatg cagtgattgt ggcttatgtt tacattgctg
    12901 ggaaagcgat ttttataata cttatcaaac ggtctttagt attttatctg ctgcagaaag
    12961 gaaaccacaa attgacttaa aagatattcc gagagaattt ttagataaat gtataaaagc
    13021 tgaagaattt atcgatacca ccaacaggat gtttgaactc tataaactca atcttctttg
    13081 gcacaataga attgtagaaa gcagggaact ggtttgtgaa cagttaaagg gcgtctctac
    13141 gataattgac agccttgcaa cagaaattta tgggaaaata tattt
//
LOCUS       DAOSUD010000059        12997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2845_length_12997_cov_23.625406, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000059 DAOSUD010000000
VERSION     DAOSUD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_12997_cov_23.625406"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      104..284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            382..1377
                     /locus_tag="PLG49_08010"
     CDS             382..1377
                     /locus_tag="PLG49_08010"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ17299.1"
                     /translation="MKKINLAIVGATGMVGRTFIKVLEEKDLPIENLYLFASSRSAGK
                     TITFKGKEYVVEELTENSFDRGIDIALFSAGGSASLKFAPIAASKGCVVVDNSSAWRM
                     NPDVPLVVPEVNPEDVKLHKGIIANPNCSTIQAVVALKPLDEYYNIKRIIYSTYQAVS
                     GAGMGGWQDLEEGIKGNPPKKFPHPIFNNCLPHIDVFTENGYTKEEIKMIEETRKILH
                     RQDLKITATTVRVPVFNSHSESINVEFEKPFDLEELKEVLRKAPGVIVQDDPENNVYP
                     LAANATGHDEVFVGRIRRDESVENGVNLWVVADNIRKGAASNAVQIAEILIKDMQ"
     gene            1393..2277
                     /gene="dapA"
                     /locus_tag="PLG49_08015"
     CDS             1393..2277
                     /gene="dapA"
                     /locus_tag="PLG49_08015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOQ17300.1"
                     /translation="MEIFRGSGVAIVTPFKENGEVNYELLEDLIEFQIQNNTDSIIIC
                     GTTGESSTLNDDDHLECIKVAVEKVNKRIPVIAGTGSNDTEHGLYLTKRAYQLGADAC
                     LLVTPYYNKTTQKGLIEHYSYIAKNVDVPIILYNVPSRTGLNITPKTAAELSKIDNIV
                     GIKEASGNISQIAELMHLCEGRLSLYSGNDDQIVPILSLGGIGVISVVANIAPKQVHD
                     LVDHYLSGNVAQSTKMQLEMIPLVKALFCEVNPIPVKAALELMGYPVGRCRMPLSTIE
                     EEHLELLKKEMKNYGLIN"
     gene            2326..3084
                     /gene="dapB"
                     /locus_tag="PLG49_08020"
     CDS             2326..3084
                     /gene="dapB"
                     /locus_tag="PLG49_08020"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOQ17301.1"
                     /translation="MIKIIMHGCNGRMGQVISEIVNEEENCTIVAGIDPDVTKSNPYP
                     VFSKIDDCDIKGDVIIDFSTASAVKPLLDYAVRMQTPVVICTTGLSDDDLELLKKSSE
                     KIPVFFSANMSLGVNLLMNLVKRASEVLANANFDIEIIEKHHNQKIDAPSGTALALAN
                     AINETLDNRYEYKYDRHQERKQRDKKEIGIHAVRGGTIVGEHSVIFAGRDEIIELKHS
                     CMSREVFATGAIKAAKFLVGKNPGLFNMENLINE"
     gene            complement(3118..3648)
                     /locus_tag="PLG49_08025"
     CDS             complement(3118..3648)
                     /locus_tag="PLG49_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOQ17302.1"
                     /translation="MEKGLIQVYCGDGKGKTTAAIGQGIRAVGQGLKVIMIQFLKIPS
                     SGEINVLKNLEPDFKIFHFERPRRFFWELNEEEKNELKNDILNGINFAKKVLDTKECD
                     MLILDEILGVIENSLFEEDELCRILDNKPDEIEIILTGRKVTEKIEERAHYVSWIQEK
                     KHPFQSGIKARKGIEY"
     gene            complement(3811..4104)
                     /locus_tag="PLG49_08030"
     CDS             complement(3811..4104)
                     /locus_tag="PLG49_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17303.1"
                     /translation="MKIKITQHMQLADFNNGWTTISKTFNSNVIPHKGDMLTDTFWKD
                     PYEYEVTEVVISYQFDECYVSVKPLVIRSNKRDVLIKYIEKAKLAGWESSLEI"
     gene            complement(4312..4953)
                     /locus_tag="PLG49_08035"
     CDS             complement(4312..4953)
                     /locus_tag="PLG49_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ17304.1"
                     /translation="MTDHNIKNNQVTVVGKVIEEIQFSHKVYGEGFYTFKVEVPRLSN
                     VSDILPVTISERLLAGKTQLLNAIVEIQGQFRSYNQYEQGKNRLILTIFALDLFIKEN
                     EEELKSINQIYLDGYICKAPVYRTTPFGREITDILIAVNRSYHKSDYIPCIAWGRNAK
                     YAQHLKVGDHLKIWGRVQSRDYQKKLENGEVTTKTAYEVSISKMEIDDNNNKE"
     gene            complement(5047..5694)
                     /locus_tag="PLG49_08040"
     CDS             complement(5047..5694)
                     /locus_tag="PLG49_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOQ17305.1"
                     /translation="MIKVGVVENQDETTTIDILAQLCKSSGKKVSILKEYNDVYRKNY
                     SFLSYINELIKNNMDMVIIHLSQEGLKNKWFKDINFDIIIYSVSSTEKENADFPLDDN
                     KFFLTLSQDAVGIINADDKELMHFFKRKINRLVTYGFNPAACITASSIQEEEINNRIQ
                     CCVKKKLKTFSGRVLEPQEFSIRLPLNENHELYSALAAITAAMLNDIKIPDKNHS"
     gene            5905..8862
                     /locus_tag="PLG49_08045"
     CDS             5905..8862
                     /locus_tag="PLG49_08045"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOQ17306.1"
                     /translation="MLLNDLLFRYYTKELENSTKTVYDLFDWKTVDVKITDHKSGKVI
                     VDMENLEFPVHYSQAACDIIASKYFRKKGVPNDRGYEYSLKQVVDRMVSFWVASALDE
                     GLFDESKKQIVYDELAYTMLNQMWAPNSPQWFNTGLKMAYGIDGPPQGHYYYDEKKQD
                     VVLSKDAYTRTQGSACFIVSVQDSLLGEKSLTDQLVTETRLFKYGSGVGSNWSAIRAK
                     GEPLSGGGKSSGLMSFLKVFDRNAGAIKSGGTTRRAAKMNILDIDHPEIEDFITWKAK
                     EEDKVVALGKMGYSTHFDGEAYETVSGQNANNSIRVTDEFMNAINDPDASIELKGRID
                     SSINKKVPVSRLWDEIAYSAWRCGDPGIQFDDTINAWHTCPAGEDGQYNAKHNRINAS
                     NPCSEYMFLDDTACNLASINIVKFYDYENDTFDLQGYLHTIKIVQIVLEATIHWGQFP
                     TKDIARKSYHFRTTGLGLTNLGALFMLMIKPYDSDEARNIAASLMSILTGYSYYISSL
                     FAEVKGPFSYFEMNKEHMLKVISNHAKAANFDEFEEDFKDLNYSPMVIDHNILLKEGY
                     QEISNCLKVVWKKALEYGKKYGYRNAQVSVLAPTGTIAFAMDCATTSSEPFFSHVVYK
                     KLVGGGSIEIVNPIIPIALRKLGYTKEETEDIINYVLRKEKRDGYEYIADGKIEGAPH
                     LLEEHYPIFDTANRCGSGKRFISPEGHVKMMGALTPHISGGISKTVNLPHDATVEDIK
                     NIYYLSWKLGVKAIALYRDGCKASQPLTTSMDEKIENKLEDLTYEELLKYAKSKQVVY
                     KPIRVKPEGIRSARVHEAEISGLRLYITTSFYEDGRLGEIYVAAGRQGSLVKGLLDSL
                     STTISEMLQYGVPPKDIARMYRGQKYEPSGFVSRHPYIKSADSISDLISKIIEIELGD
                     FSHCQVKPQDLAQGNHNNSQKEFRIEAIEPESSEPPEILYGEICPECKSSKLVRNGTC
                     KVCLECGTTTGCS"
     gene            complement(8889..9629)
                     /gene="yunB"
                     /locus_tag="PLG49_08050"
     CDS             complement(8889..9629)
                     /gene="yunB"
                     /locus_tag="PLG49_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HOQ17307.1"
                     /translation="MRRRIKGKKPKRVKNIYKKKIRFSVITFLILILLLLFYIRINSK
                     IMPTVMTIAHIKANSIATEAISKAVNDAFRKKNITAQDLVVYDYNDEGEIVSWSINTP
                     VINELCADIVVGITKELENSPKTTIKVPLGNITEGKIFANIGPKISIQVLPTGSAVIN
                     YDKEFRSTGINQINHTVWLNVDTVVKVVIPMSSEEIRVSRKVILVDKVLSGKVPPSYV
                     DTTQDSILDAELEDPFEPPKAFEYPSVN"
     gene            9879..12614
                     /locus_tag="PLG49_08055"
     CDS             9879..12614
                     /locus_tag="PLG49_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOQ17308.1"
                     /translation="MNFSKESNAKKKKAINAKRKKVKNRLGLIVFRFIFALFIIAIFA
                     GVGGALGALFGIINTAPDVDTIQLSPERYTSIIYDLNGNELDRLHGDENRVYAELDEI
                     PLYLQQAFIAIEDERFYSHNGVDVKGMMRALYVNIKERNFSEGASTITQQLVKNRVLS
                     KEKKIKRKLQEQYLAIQLEKKYSKEQILEWYLNEIALGRGYNGVKSAARGYFNKEVSE
                     LTLAESAVIAAITQNPSYYDPIRFPENNRVRQTIVLDKMLEQGYITQSQYNEAINEDV
                     YQKIQEASELFLEDSKHTYYIDQVISDVMRDLQIKKGYTANEAEFLIYSGGLSIFTPF
                     DQNIQDIVDKHYQNDDLFPPKSYELKIIYKLSVEKPNGDVKNFEAEEIVKNEEAIESL
                     KAQKKEEWEVTENDKVLGEALYKIPQPQSAMVIMDYHNGHVLAIAGGRGEKVGNLLFN
                     RATQAKRQPGSAFKVLAAYAPALDTGKITPSTVIDDAPLKVKDGSGYKYINNWTGTYK
                     GLSTVREGIYNSMNVLAVKTLLMTGIDTCFDYLQHFGFTTLVDREERNGYVFSDKNPV
                     LALGGITYGVTPLELTAAYATIANGGVYNEPIFYTKILDHDGNLLLENVPEQRRVLKE
                     STAYLLTDMMQDVVTKGTGQLARMKSMPVAGKTGTTSDDKDLVFVGYTPYYVAGIWLG
                     HDTPKRLRYDRSYHLLLWKAIMEDIHEGLPVKKFPSTEGIVQAKICTKSGKLATTTCE
                     HDPSGSTIRYEYFASGTQPKEYCDVHTVRKVCTVSGKLANEYCPEDVVKEKVFIKRPI
                     PLDPNAGAVPEDAQYELPSEENEFCDVHGPHTVTDPYYPDYYDIPEADENGNIVPYDP
                     NLNNPVPNTPTPNIPDNNNSQRQERNNPQKREQNTTSSTTNYTPISQDDFYVPLP"
     gene            complement(12652..>12997)
                     /gene="spoVAE"
                     /locus_tag="PLG49_08060"
     CDS             complement(12652..>12997)
                     /gene="spoVAE"
                     /locus_tag="PLG49_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOQ17309.1"
                     /translation="KAFIVGGAICVFGQILIDKTKLMTARILVLFVVLGAVLHGIGLY
                     EPLVKFAGAGATVPLPGFGYSLAKGAMEEVDKIGLKGAFTGGIKSTAAGITAAIVFAY
                     LAALIFNPKAKD"
BASE COUNT         4337 a         2374 c         2262 g         4024 t
ORIGIN      
        1 tggttattcc caagataaaa tatacatatg attataaaaa ggcttcaaaa acaattgaca
       61 gatttacata tgaatgatac aattaaacaa atttcatatt ggtacgcaag atagaggcgc
      121 attttctaag agtatatata cggatgtaag tgggtacaga tgaagtatat agaaaggaga
      181 aaatgccgaa gagctttaaa ccctactgtt tactgctctg gttgtgcagt taagagctgt
      241 acgactgtca tcgtaattaa tttcgatgga gagctatctt cttaatgctt tatgaatgca
      301 ttaataggtt ggcttatgcc aacttttttt gttaaaaatg ttaatcattt aaatttaaaa
      361 taaatttaag gaggcgtaat catgaaaaaa attaacttag caattgtagg tgctactgga
      421 atggttggaa gaacttttat aaaggttctt gaagaaaaag atctccccat tgaaaattta
      481 tatttatttg cttcatcccg ctctgctggg aaaacaataa cttttaaagg taaagaatat
      541 gttgttgaag aattaacaga aaactctttt gacagaggaa tagacatcgc attattctcc
      601 gctggcggat ctgcaagctt aaaatttgct cctattgcag ctagtaaagg ctgtgttgta
      661 gtagataaca gctctgcatg gagaatgaat ccagatgtac ctttagtcgt tcctgaagtt
      721 aatcctgaag atgttaaact tcataaagga attattgcta atccaaactg ttccactatt
      781 caagcagtag tggcattaaa acctcttgat gaatattata atattaaacg cataatttac
      841 tcaacttacc aggctgtatc cggagccggc atgggaggct ggcaagattt agaggaaggt
      901 ataaaaggta atcctccaaa gaaattccct catcctatct tcaataactg tttacctcat
      961 atagatgtct ttacagaaaa tggttacact aaagaagaaa ttaaaatgat tgaagaaacc
     1021 aggaaaattt tacacagaca agacttaaaa attactgcaa cgactgtaag agtacctgtg
     1081 tttaatagcc atagtgaatc tatcaatgtg gaattcgaaa agccctttga tttggaagaa
     1141 ttaaaagaag tcctcagaaa agcacctggt gttattgttc aggatgaccc ggaaaacaac
     1201 gtttatcctt tagctgctaa cgcaactggt catgatgaag tctttgtggg tagaatcaga
     1261 agagacgaaa gcgttgaaaa tggtgtaaac ctttgggttg tagctgacaa tatcagaaaa
     1321 ggtgccgcat caaatgcagt tcaaattgct gaaatcctga ttaaagatat gcaataagaa
     1381 agggtggtat agatggaaat cttcaggggt tctggtgtag cgattgtcac cccttttaaa
     1441 gaaaacggag aagtcaatta tgagttgctg gaagatttga ttgaatttca gattcaaaat
     1501 aatacggaca gtatcattat ctgtggtacc accggtgaat cttctacttt aaatgatgat
     1561 gaccatttgg aatgtattaa agtagctgtc gaaaaagtta ataaaagaat tccggtcatt
     1621 gccggaacag gaagtaatga cactgaacat ggcctgtact taaccaaaag agcttatcag
     1681 ttaggcgctg acgcttgtct tctggtaacc ccttattaca ataaaacaac ccaaaaaggt
     1741 ctaattgaac actatagtta tattgcaaaa aatgttgatg tacctattat attgtataac
     1801 gtaccttcaa ggactggttt aaatattact ccaaaaacag ctgctgaatt atccaaaatt
     1861 gataatattg ttggaattaa agaagctagt ggaaatatca gtcaaattgc agaattgatg
     1921 catttatgtg aaggaagact cagtttatat tcaggaaatg acgaccaaat cgttcccatt
     1981 ttatccttag gaggaatcgg tgttatctct gttgttgcaa atattgcacc aaagcaagtc
     2041 catgatcttg ttgaccatta tcttagcggc aatgtggccc aaagtacaaa aatgcaatta
     2101 gaaatgattc ctcttgtaaa agctctgttt tgtgaagtga acccaattcc tgtaaaagct
     2161 gctcttgaac ttatgggata tcctgttggc cgatgcagaa tgccacttag caccatagaa
     2221 gaagaacatc ttgaactact taaaaaagaa atgaaaaatt acggcttaat caattaatta
     2281 aagtgcagag gttctccctg cactttttac gaaaggagct aaaatatgat aaaaatcatt
     2341 atgcacggtt gtaatggaag aatggggcaa gtcatttcag aaattgtgaa tgaagaagaa
     2401 aattgtacca ttgttgcagg cattgaccct gatgtgacaa aatctaatcc ttaccctgtt
     2461 tttagcaaaa tagatgattg cgatataaaa ggagatgtta tcattgattt ttcgacagct
     2521 tctgcagtaa aacccttact cgattatgcg gttaggatgc aaacacctgt cgtaatatgc
     2581 acaacaggct tatcagacga tgacctggaa ctccttaaaa aaagctctga aaaaattcct
     2641 gtattttttt ctgcaaatat gtcccttggg gtcaaccttt taatgaatct ggtaaaaaga
     2701 gcaagtgaag tcttagccaa tgctaatttt gatattgaaa tcattgaaaa acatcataat
     2761 caaaaaatag atgcacccag tggaactgcc cttgctttag ccaatgccat taacgaaaca
     2821 ctggataacc gttatgagta taaatacgac cgccatcagg aaagaaaaca acgggataaa
     2881 aaagaaattg gtattcatgc tgtcagaggg ggtaccattg ttggtgagca ctccgttatc
     2941 tttgcaggtc gggatgaaat tattgaactt aagcattcat gcatgtccag agaagtcttt
     3001 gctactggag caattaaagc cgctaaattt ttagtaggca aaaatcctgg actctttaat
     3061 atggaaaact taattaacga ataaaaaaat ggggttcgtc atacggacct cattttttta
     3121 atactctatt ccttttcttg cttttatacc tgactgaaag ggatgctttt tttcctgaat
     3181 ccaggataca taatgggctc tttcttcaat tttttcagtt acctttcgac ctgtcagaat
     3241 aatttctatt tcatcaggtt tattatccaa tatcctacat aattcatcct cttcaaacaa
     3301 tgaattttca ataacgccta gtatttcatc caatattaac atatcacatt cctttgtatc
     3361 caaaactttt ttagcaaaat ttatgccatt taaaatatca ttttttaatt catttttttc
     3421 ctcttcattg agttcccaaa aaaatcttct gggacgttca aaatgaaaaa ttttaaagtc
     3481 cggctccaaa ttctttaaca catttatttc gcctgaagaa ggtatcttaa gaaactgaat
     3541 cataatgacc ttcaagcctt gcccgacagc tcttatcccc tgtcctatgg cggccgttgt
     3601 ttttcccttt ccatccccgc aataaacttg aatcaaccct ttttccattt tttcacctct
     3661 tccttcacat taacattatt attatatatt tagttttatt tttttcaagc aaaaaaagaa
     3721 aaacatcttt ttcttcaaga tgtttttctt agtgtcttct ctgtcttgtt actataatta
     3781 tattacatcc tttgtcattt cattccatat ttatatctcc aaactggatt cccaaccagc
     3841 cagctttgct ttttctatat attttattaa aacatctctt ttattacttc ttatcaccag
     3901 tggcttaaca ctgacataac attcatcaaa ctgataacta attacgactt ccgttacctc
     3961 atattcataa ggatccttcc aaaaggtatc cgttaacata tcacctttat ggggtataac
     4021 atttgaatta aaagtcttgg aaatagtagt ccaaccatta ttaaaatcag ccagctgcat
     4081 atgttgagta attttaattt tcatcaaatc tcccccttag aattaatcct ctctttcaga
     4141 atttcgacaa taaaggggaa attccttctt ttttataaaa ttttttattt gtttactaca
     4201 ttcatatcaa accttagaaa atcttcttta ctccaatccg gttttttctg accttcgtcc
     4261 tatgcttact ttaaatctcc tgcttctaac gttccaatcc agcctccggt ctcattcctt
     4321 gttgttattg tcatcaatct ccatcttcga tattgagact tcataagccg ttttagttgt
     4381 cacttcacca ttttcaagtt tcttctgata atccctgctt tgtacccttc cccaaatttt
     4441 aagatgatcc cctactttta aatgttgggc atattttgca tttctgcccc aggcaataca
     4501 aggaatataa tcagatttat ggtaagagcg gttaacagca attaagatat cggttatttc
     4561 tctgccaaat ggcgttgttc tatatacagg tgctttacaa atataaccgt caagatatat
     4621 ctgattaatg ctttttagct cttcttcatt ctctttaata aataaatcca aagcaaatat
     4681 tgtaagaatc aagcggtttt taccttgttc atactgatta taagagcgga attgaccttg
     4741 tatctcaaca atggcgttta ataactgcgt tttcccagcc aataacctct ctgaaatggt
     4801 tacaggcaag atgtctgaca cattacttaa tcttggaacc tccactttaa aagtataaaa
     4861 gccctctccg taaactttgt gactaaattg gatttcttca attactttcc caacaacagt
     4921 aacttgattg tttttaatat tatggtctgt cacttatttc acctctccct tattatctga
     4981 aagttcttta agtgattttt ctatcattaa catctttatt cgtaagacat aaattttaga
     5041 agtaatttaa gaatgatttt tatctggtat ttttatatca ttaagcatag cagctgtaat
     5101 agcagccagt gcactgtaca attcatgatt ttcgttaaga ggcaatctga tggaaaattc
     5161 ctgaggttct aaaaccctgc cgctaaaagt tttaagcttt tttttcacac agcattgaat
     5221 tctgttgtta atctcttctt cctggatact ggaagccgta atacaggcag caggattaaa
     5281 accataagta accagtctgt tgatttttct tttaaaaaaa tgcattaatt ctttatcatc
     5341 tgcattgata attccaactg catcttggct taaagtcaaa aagaatttat tatcatccag
     5401 aggaaaatct gcattctctt tctcagtact ggatacagaa taaattataa tatcaaaatt
     5461 tatatcttta aaccatttat tttttaaccc ttcttgtgat aaatggataa tcaccatatc
     5521 catattattt ttaattaact catttatata acttaaaaaa gaataattct ttctgtatac
     5581 atcattatac tcttttaata tgcttacctt cttacctgaa gatttacaaa gctgagccag
     5641 gatatcaatg gttgtcgttt cgtcctgatt ttcaactact cctaccttaa tcataccaaa
     5701 gctccttttt tagtcataat aatttattaa tagtttttgt attatatttt ttttatgtgc
     5761 atttttcgtc aaaatccttc tttattaagt tccctaaaaa atttaatatc aaaatctttt
     5821 ttattatttt taaacaatat attgaattat ttattcgtaa tgatacaata tatagtgtat
     5881 tataaaaata gggggggatg gtttatgtta ttaaacgatc tactttttcg ttattacaca
     5941 aaagaacttg aaaattctac aaaaaccgta tacgaccttt ttgattggaa aactgtcgac
     6001 gtaaaaatta ccgatcacaa aagtggaaaa gtcatcgtag acatggaaaa tctagaattt
     6061 cctgttcatt attcccaggc tgcctgcgat attattgcca gcaagtattt tagaaaaaaa
     6121 ggcgttccca acgaccgcgg atatgaatat tccctaaaac aagtcgttga cagaatggtt
     6181 tccttttggg tagcctcagc cttagacgaa ggtttatttg acgaatcaaa aaagcaaatt
     6241 gtttatgatg aactcgctta caccatgtta aatcaaatgt gggcacctaa tagtccccaa
     6301 tggtttaata ctggcttaaa aatggcatat ggcattgatg gccctcctca agggcactat
     6361 tattacgatg aaaaaaaaca agatgtcgtt ctttctaaag atgcttatac cagaactcaa
     6421 ggttctgcat gctttattgt cagtgttcag gattccttgt taggcgaaaa atctctgact
     6481 gatcaactgg ttactgaaac ccgcttattt aaatacggtt caggggtcgg cagcaactgg
     6541 tctgccatta gggccaaagg agaacctcta tccggtggtg gaaagtcttc aggtcttatg
     6601 agctttttaa aggtatttga ccgaaatgcc ggcgctatca aatctggagg aacaaccagg
     6661 agagccgcaa aaatgaatat cctggatatc gatcatcctg aaatagaaga ttttattacc
     6721 tggaaagcta aagaagaaga taaagtagtt gcccttggaa aaatgggtta ctctactcat
     6781 tttgacggag aagcctatga aacggtttca ggtcaaaatg caaacaattc catccgggtt
     6841 acagatgaat ttatgaatgc aataaacgat cccgatgcaa gcatcgaatt aaaaggaaga
     6901 atagattctt ccatcaataa aaaagtacct gtttccagac tttgggatga gattgcttat
     6961 tctgcctgga gatgcggaga tccgggaata caatttgacg ataccatcaa tgcctggcat
     7021 acctgccctg ccggagaaga tggacaatat aatgcaaaac acaaccggat taatgccagc
     7081 aacccttgtt cggaatatat gttcttagat gacacagcat gtaatcttgc aagtattaac
     7141 attgtaaaat tttatgatta tgaaaatgac acctttgatc tacaaggtta tcttcatact
     7201 ataaaaattg tccagattgt acttgaagca acgattcact ggggacaatt ccctacaaaa
     7261 gatattgcca gaaaatctta tcatttcagg acaactggtc ttggattaac caacttaggg
     7321 gcacttttta tgctcatgat aaaaccttac gactcagatg aagcaagaaa tatcgctgct
     7381 tctttaatga gcatcttaac aggatattcc tattatattt cttccttgtt tgcagaagta
     7441 aaaggtccct tcagctattt tgaaatgaat aaagaacata tgctgaaagt catcagcaat
     7501 catgcaaaag ctgcaaactt tgatgagttt gaagaagatt ttaaggacct gaactattcc
     7561 cctatggtca tagatcataa tattcttctg aaagaaggtt atcaggaaat ctccaactgt
     7621 cttaaagttg tatggaaaaa ggccttggaa tacggcaaaa aatatggcta cagaaatgca
     7681 caggtttctg ttttagcgcc tacaggaacc atcgcttttg ccatggattg tgcaaccacc
     7741 tcctcagaac ccttttttag ccatgtcgtt tataaaaaat tggttggcgg gggcagtatt
     7801 gaaattgtta atcctattat tccaattgct ttaaggaaac tgggatatac gaaagaagaa
     7861 acagaagata tcataaatta tgtattgaga aaggaaaaaa gggacggtta tgaatatata
     7921 gcagacggta aaattgaagg tgcaccacat ttattagaag agcactaccc tatttttgat
     7981 acggcgaacc gctgtgggag cggaaaaaga tttatcagtc cagaaggtca tgtaaaaatg
     8041 atgggtgccc tgacccccca tatttcagga ggcatcagca aaacggtcaa tctgcctcat
     8101 gatgccactg tggaagatat caaaaatatt tattatctat cctggaaatt aggagtcaaa
     8161 gccattgccc tttacagaga cggctgtaaa gcatcccagc cgttgaccac cagtatggat
     8221 gaaaaaattg aaaataaatt agaagatttg acttatgaag aactgctcaa atacgcaaaa
     8281 agtaaacaag tggtttataa acccatcagg gtaaaacctg aaggcatccg gtctgccaga
     8341 gttcacgaag ctgaaatatc aggattaaga ttatacatca ccacctcttt ttacgaagac
     8401 gggagactgg gtgaaatata tgtagctgca ggtcgtcagg gctccctggt aaaaggtctg
     8461 cttgacagtt tatcaacaac tatttctgaa atgcttcagt atggcgtacc cccaaaagat
     8521 attgcgagaa tgtatcgtgg acaaaaatat gagcccagcg gttttgtaag cagacatcct
     8581 tatattaaaa gtgccgattc tatttccgat ttaattagta aaattattga aattgaatta
     8641 ggagacttct cccactgtca agttaaacct caggacctgg cccagggcaa tcataacaac
     8701 agtcaaaaag aatttaggat agaagccatt gaaccagaat catcagaacc cccagaaatc
     8761 ctatatggag aaatttgtcc cgaatgtaag agttcaaaac ttgttcggaa tgggacttgt
     8821 aaagtctgct tagaatgcgg gacgacaact ggatgtagtt aagaaaaagc atgccctggc
     8881 atgctttttt aatttactga ggggtactca aaagctttgg gtggttcaaa aggatcttcc
     8941 agttctgcat ccaggatact gtcctgagtt gtatctacat aacttggcgg tactttacca
     9001 cttagaactt tatcaaccag aataactttt ctgctaaccc ggatttcttc agaagacata
     9061 ggaataacaa ccttaacaac cgtatctaca ttaagccata ccgtatgatt aatttgatta
     9121 atccctgtgg aacggaattc tttatcataa ttaatgaccg ctgaacctgt aggtaagacc
     9181 tggatactta tcttaggtcc tatattggca aatatttttc cttctgtaat gttaccaaga
     9241 ggaaccttga ttgtggtttt agggctgttt tctaattcct ttgtaatacc tactacaata
     9301 tctgcacata attcatttat aactggtgta ttaatcgacc aggaaacaat ttctccttca
     9361 tcattataat catatacgac taaatcctgg gctgttatat tcttttttct aaaagcatca
     9421 ttaacggctt tacttatggc ttctgttgca atggaatttg ctttaatatg tgctatagtc
     9481 ataactgtcg gcataatttt actgtttatt ctgatgtaaa atagtaataa taatattaaa
     9541 attaaaaaag taataacaga aaatctaatt tttttcttat aaatattttt aactctttta
     9601 ggttttttac ctttaatcct tctcctcatc ctaccccccc tcttacaatt tatgcagtag
     9661 ttataaaaat gttccgataa aaataatata ttattagtct ttttaaactt gcctatatct
     9721 gttataatat agtgaggcaa atttgcctac atatatttaa cagcaaaggt aagactttta
     9781 attaaattat ttttgtatac tcatacgcat taaagtatat gcatagtatg gatgatatgg
     9841 ctaagaatca ttatccatat caaatgagga ggcttaaaat gaatttttca aaagaatcca
     9901 atgctaaaaa gaaaaaagca ataaatgcaa aaagaaaaaa agttaaaaac agacttggac
     9961 tcatcgtatt cagatttatt tttgctttat tcatcattgc catatttgca ggcgtaggcg
    10021 gggccttggg cgcattattt ggtatcatta atacagctcc cgatgtggat acgattcaac
    10081 ttagtccaga aaggtatact tctattattt atgacttaaa tgggaacgaa ctcgacaggc
    10141 ttcacgggga tgaaaacagg gtttacgctg aactggatga aatccctctg tatttacaac
    10201 aagcttttat tgctattgaa gacgaaagat tctattccca taacggtgtc gatgttaagg
    10261 gtatgatgag agcactttat gtgaatatta aagagcgaaa ctttagtgaa ggtgcaagta
    10321 ctattaccca acaattagta aaaaacaggg tattatcaaa agagaagaaa atcaaaagaa
    10381 aacttcagga gcaatattta gccattcagc tcgaaaagaa atacagtaaa gaacaaattt
    10441 tagaatggta tctcaatgaa attgctcttg gcaggggata taacggtgtt aaatctgccg
    10501 caagaggcta cttcaataaa gaagtcagcg aattgactct ggcagaatca gcagttattg
    10561 ctgctattac ccaaaatcct tcttattacg accctatcag atttccggaa aacaacaggg
    10621 taaggcagac cattgtcctg gataaaatgc tcgaacaagg ttatatcacc caaagtcaat
    10681 ataacgaagc catcaatgaa gatgtctatc aaaaaattca ggaagccagt gaattattcc
    10741 tggaagattc caaacataca tattatattg accaggttat cagcgatgtt atgcgggatc
    10801 ttcagattaa aaaagggtat acagccaatg aagcagaatt cttaatttac agcggaggac
    10861 tgagcatttt tactccattt gaccagaata ttcaagatat cgtagacaaa cattatcaaa
    10921 acgatgacct cttcccacca aaatcttacg aattaaaaat catttataaa ttatctgttg
    10981 aaaaaccaaa tggagatgtt aaaaactttg aagcagaaga aattgttaaa aacgaagagg
    11041 ctattgaaag cttaaaagcg caaaagaaag aagaatggga agtaacagaa aatgataaag
    11101 ttttagggga agccttgtac aaaatacctc aacctcaatc cgcaatggtc attatggatt
    11161 accataatgg acatgtattg gccattgccg gagggcgtgg agaaaaagta ggtaatctgt
    11221 tatttaaccg ggcaactcag gcaaaaagac agcccggatc tgcttttaaa gttttagcag
    11281 cctatgctcc tgctctcgat actggaaaaa tcacaccttc aaccgttatt gatgatgcac
    11341 ctttaaaagt taaagatggt tctggatata aatacatcaa taactggacc ggtacttata
    11401 aaggtttaag tactgtcaga gaaggtattt ataactccat gaatgtatta gcagttaaaa
    11461 cgcttttaat gacaggtatt gatacctgtt ttgattactt acaacatttt ggctttacta
    11521 cccttgttga tagagaagaa cgcaacggct atgtcttttc agataaaaat cccgtattag
    11581 ccctgggtgg aataacatat ggcgttacac ctctggaact gactgctgct tatgcaacca
    11641 tagcaaacgg gggcgtatat aatgaaccga ttttctatac caaaatcctg gaccatgacg
    11701 gaaatctcct tttagaaaat gtacctgaac aaagaagagt tctcaaagaa tccactgcat
    11761 atcttcttac agacatgatg caagacgtcg taacaaaagg tacaggacaa cttgcccgca
    11821 tgaagagtat gcctgtggct ggaaaaacag gaacaacctc agatgataag gacttagtct
    11881 tcgttggata tacaccttat tatgttgcag gaatctggtt gggacacgat acaccaaaaa
    11941 gactccgcta tgacaggagt tatcatctgc ttctttggaa ggccattatg gaagatatcc
    12001 atgaaggctt accagttaag aaattccctt ccactgaagg aattgtgcaa gctaaaatat
    12061 gtacaaaatc cggaaaactg gctacaacaa cttgtgagca tgacccgtca ggctcaacca
    12121 tccgctacga atactttgcc agtggaaccc agcctaaaga atactgtgat gttcacaccg
    12181 taagaaaagt atgtaccgtt tcaggaaagt tggctaatga atactgtccg gaagatgtgg
    12241 ttaaagaaaa agtatttatc aaacgaccaa tacctttaga tcctaatgcc ggtgcagtac
    12301 ctgaggatgc tcagtatgaa cttccttctg aagaaaacga attttgtgat gtacatggtc
    12361 cacatacggt aacagatcct tattatccgg attattatga tattcctgaa gcagatgaaa
    12421 acggaaacat tgtaccatat gatccaaatt taaataatcc tgtaccaaat accccaacac
    12481 caaatatacc tgataataat aatagtcaaa gacaggaaag aaataatcct caaaaaagag
    12541 aacaaaatac aaccagctcc actacaaatt atacgccaat tagtcaagat gatttttatg
    12601 ttcctttacc ttaatcataa aaaagactag tctttcagaa tatcagaaag actagtcttt
    12661 tgcttttgga ttaaaaataa gtgctgccag ataggcaaat acaatagctg cagttatgcc
    12721 tgctgctgtc gatttaatac cacctgtaaa agcaccttta agacctatct tatcgacttc
    12781 ttccatagca ccttttgcca gagaatatcc aaagccaggt aaaggaactg tggccccggc
    12841 acctgcgaac tttaccaaag gctcatataa acctattcca tgaagaacgg cacccaaaac
    12901 aacaaacaag actaaaattc ttgctgtcat aagctttgtc ttatcgatta aaatttgacc
    12961 aaaaacacaa atggcgcctc ctactataaa agcttta
//
LOCUS       DAOSUD010000060        12619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2918_length_12619_cov_24.891515, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000060 DAOSUD010000000
VERSION     DAOSUD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_12619_cov_24.891515"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1135
                     /gene="metA"
                     /locus_tag="PLG49_08065"
     CDS             230..1135
                     /gene="metA"
                     /locus_tag="PLG49_08065"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOQ17310.1"
                     /translation="MPIKIPNDLPAKQVLENENIFVMDETRAVTQDIRELKIVILNLM
                     PKKQETEVQLLRLIGNSPLQVEITLLHPKTHKSKNTSEEYLLAFYHTFDDIKHKKFDG
                     MVITGAPVETLSFEEVTYWEELKEIMEWTKSNVTSTLHICWGAQAGLYYHYGVPKYPL
                     PKKMFGVFPHYVLKNAKLLQGFDDVFYVPHSRHTYTRREDIEKIEALELLSVSDESGV
                     YIAASKDGKQIFITGHSEYDPNTLKDEYERDLSKGLDIEIPINYFPDNDVTKNPVVTW
                     RSHANLLFSNWLNYYVYQVTPYHLD"
     gene            1307..1948
                     /locus_tag="PLG49_08070"
     CDS             1307..1948
                     /locus_tag="PLG49_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ17311.1"
                     /translation="MKFAGLKEYVYHEIKQRLINNEIKPGERIWEEEIAAELEVSRTP
                     VREAINRLIAEEFVENRPRKGIFAAEISKEELRKMLDVRIALESLSVSQCCKLISEEE
                     KDELRAIFGDYKRKMEEGEYEKASQLDSKMHRFIAKIADNKKLEAYINDIQDFFAYSR
                     PYTVKWTEAKIKRAINDHKELIDALCNGNEERAIEIIREDIEAMRDLLKDNNE"
     gene            2209..2844
                     /locus_tag="PLG49_08075"
     CDS             2209..2844
                     /locus_tag="PLG49_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ17312.1"
                     /translation="MKFSGLKEYAYHKIREKLISNEIKPGERIWEEEIAAELGISRTP
                     VREAINRLVAEGLVGNRPRKGVFAAEISKNELRKMLSVRTVLETLSVSQCCKLITEAE
                     MYELKKIYDNYKSAMEKGDYEKASQLDSQIHRYIAKISGNRRLENYINDIQDFFAYAR
                     PHKVKWTETKIKRSLQDHKKLVDAICNRDEDSAIAIIKEDIAAMDDLLKNS"
     gene            2875..3705
                     /locus_tag="PLG49_08080"
     CDS             2875..3705
                     /locus_tag="PLG49_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOQ17313.1"
                     /translation="MKLCYQVATPDVAIADSVTAYQGPLEKSFYDLASYGYDGVELMT
                     LNPDELDWDFVKKEAEKNKLAVVLVCTGEIFGQLGLSYTNPKEEIRKEAIRRTKGIID
                     FAAFLGANINIGRIRGQYCKELSKEQTEKYAIDAFKELSDYSGKKNVQIALESVTIMQ
                     TNFINTLEEAVLMIKAVDNPYFKLMMDVFHLNLEEKDICEAIRNYSPYNIHVHLADNN
                     RRYPGHCGLDFEKIIKTFKECGYDGNYCTEIFQIPSQEEAAKGAIQHLRPIFNKVYGK
                     "
     gene            3727..4554
                     /locus_tag="PLG49_08085"
     CDS             3727..4554
                     /locus_tag="PLG49_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ17314.1"
                     /translation="MTKERKKELQDLCLKFRHDLIDILHSIQTGHPGGSLSCTEIVTT
                     LYFEKMNIDPKDPQKEGRDRFILSKGHACPMLYLNLAERGFFPKEDLKHLRQTDSHLQ
                     GHPCFHKTPGVELSTGPLGMGLSAGLGMALGEKLKKTDAKIYVLLGDGEIQEGGIWEA
                     AMAASKFKADNLIAILDYNGVQLDGTLEEIMPLGDVASKFESFGWNVLHCDGHDVESV
                     SAAIDKAKESTDKPSMIIARTVKGKGISFMEGKNTWHGKPISDEEYKMAKAELGGGN"
     gene            4554..5489
                     /locus_tag="PLG49_08090"
     CDS             4554..5489
                     /locus_tag="PLG49_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOQ17315.1"
                     /translation="MSKQIAIRDAYGEALLELGKKNEKIVALEADVGASTKSILFGEV
                     FPDRYFNVGISEFNMVAMAAGFAQEGFIPFVNTFAVFMTTRASDPIQSLIAYDKLNVK
                     MAGTYAGLSDSYDGASHQAITDIAFVRSIPNMTVISVSDAVETKKAVEAIAEYDGPVY
                     LRLSRAPAPVIFDENYDFQIGKGVELRTGKDVAIIATGTILHNALKAAELLEEEGIEA
                     TVINIHTIKPIDEEIIINAARLTGAVLTVEEHSIYGGLGSAVAEVLSQNYPTPVDFVG
                     ATTFAESGDYDELLEKYGYGARTIADKAKQLINRK"
     gene            5521..6417
                     /locus_tag="PLG49_08095"
     CDS             5521..6417
                     /locus_tag="PLG49_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOQ17316.1"
                     /translation="MRESIHKYFQIGTIQFMSYPGIDPVESIKKIACDDYFDAIEITK
                     VDGEEKRAQVKKMLIESGLKVCYGAQPLLLGTKLNPNAIDEEERKKAEELLLKAIDDA
                     EYFGAKGIAFLAGKWEKETKDQAYEQLLKTTRNLCDYAATKGMHVNLEVFDFDMDKAA
                     LIGPAPYAAKFAADMRTTHSNFGLMVDLSHFPTTYETARFVIRTLRPYIAHFHIGNAV
                     VKEGCEAYGDMHPRFGFPNSANNIEELLEFFRVLKEEGFLDSKEPYVLSFEVKPWKDE
                     DPDIILANTKRVINRAWALLED"
     gene            6467..7426
                     /locus_tag="PLG49_08100"
     CDS             6467..7426
                     /locus_tag="PLG49_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ17317.1"
                     /translation="MKIVILDGYTENPGDLSWEGFEKLGELTVYDRTPADKIIERIGD
                     ADVVYTNKTPISRETLDACPNVKFIGVLATGYNVVDVEAAKEKGIPVSNIPTYGTDAV
                     AQFTIALLLELCHHIGAHSDAVKNGEWTNNPDWCFWKYPLIELAGKTMGIIGFGRIGQ
                     RTAEIAQALGMKILAYDAYKNSALESETCKYAELDELLANSDVISLHCPLFPETQGII
                     NKDTIAKMKDGVMILNTSRGPLIVEEDLRDALNSGKVAGAALDVVSKEPIEMNNPLLS
                     AKNIIITPHIAWAPKESRQRLMDIAVDNLKAFISGNPQNVVNK"
     gene            7521..7970
                     /locus_tag="PLG49_08105"
     CDS             7521..7970
                     /locus_tag="PLG49_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOQ17318.1"
                     /translation="MTKFKVKNLIPLLFVVMGLVFAILGFTQLGFWSDVDGPKPGFFP
                     SIIAIVMVFTGLIAFIQSFKEEDKAQYNAQEFLAMAGGLGIFAATFIIGLVPTIILYI
                     ILWLKLVEKASWKVILIVLAIACFITIGVFGIWLGIQFPKGIFEFIL"
     gene            7989..9497
                     /locus_tag="PLG49_08110"
     CDS             7989..9497
                     /locus_tag="PLG49_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOQ17319.1"
                     /translation="MSNFEMLLRGFSVVFTPANIGASILGAILGLIVGAMPGIGSLAG
                     VALLLPLTYKFNPTTAIIMLGALYYSNMYGGSFSAILLNIPGDSPAVMTAVDGYPMAK
                     KKKRPGQALFTANMASCIGGTIGMIILTFAGPALANIGLKFGPSEMTALLLVAMTSIS
                     WLVGENPIKGIVATAFGILLASMGMDTLSGSPRYDFGSMHLLGGIPFTPFVIGSVGFA
                     QVINLMNERYVQTEMIDAKLSIRGSLPTKREFLRLIPPSIRSGILGTFVGVLPGAGAT
                     TGAFMGYAIQKNFKSDEPLGTGAVEGIAACEAANNAAAAGSFAPLLALGIPGSGTGAV
                     LLGGLMLWGLNPGPLLFQNEPDFAWGLISSLFLSNAITLLIAIAVIPFLTKILAVPTK
                     YMIPIITVVCVVGSYSTTYSMFGVLVMLLSGIVSYFIQKNNYSTAPMLLSFVLAPLLE
                     SNMRKAFIISGGSLKIFFTRPISLMFMLIFFTMLLIPLVRSILGKKKEEKTA"
     gene            9583..10629
                     /locus_tag="PLG49_08115"
     CDS             9583..10629
                     /locus_tag="PLG49_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOQ17320.1"
                     /translation="MKNKMMALLAGVLTVSVLFTGCQKSGGNSSSGGTSSGGTSSGEK
                     TTVTKEFKPASTVDWYVASSPGGGSDIFTRMITDIMISEGIVDTNFIVTNKTDGGGEV
                     VRNEVAHMSKGDRANNTLLTFNSGCLMPMVENTKNRSSNFKVIAVMAVDKQLLFKTEH
                     TKYADFNEAIEAAKSGASIVIGGSKGDDVATYEALIQEIGVSADNMKYITYDATSDAI
                     TAGLGGHVDFVMSKPAAASEYVEAGSLIPVLALSKERFSGNLADAPTLSEIGDYEDVE
                     VPVWRSVVAPASMSDEAVAFWTDALKKVSETEKWQNDYIAKYKLIPEYMDAATATGYI
                     SEFEKNFMASKGIQ"
     gene            10768..11769
                     /locus_tag="PLG49_08120"
     CDS             10768..11769
                     /locus_tag="PLG49_08120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOQ17321.1"
                     /translation="MEKKPIIGIILGDAAGVGPEIIAKLAARKFYDEYCRPVILGDAR
                     VFEEGARVAGVEVPVQIIEDVDDATWEKGIPVLDQKDLDPANIPYGQLNVESGKACLD
                     LLKLAVELFQAEKIDGFCFGPLNKSAMIAAGCPYESEHHYMAALFNHTEPFGEINYLD
                     GLWTTRTTSHIPIKEVSNHLTVDRILRAVRLANTTLKNAGYDKPVLGLAALNPHCGEN
                     GKCGREEIDVIAPAIEKAIEMGIDARGPYPADILFIKAFNGEFDGVVTMYHDQGQIAL
                     KLKGFDYGITIAGGLPAPIVTCAHGTAYDIAGKGIAKTTAFENAVKMAARMASHIRG"
     gene            11784..>12619
                     /locus_tag="PLG49_08125"
     CDS             11784..>12619
                     /locus_tag="PLG49_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOQ17322.1"
                     /translation="MNLSMRKYMKVGIILHMAFDGLISGEGPILECLEKIAADEYFEV
                     VEITQIKDPGVRKAVREMVETAHMELIYGGQPRLLTKGLNINDLDEEKRRLAVEELKD
                     GIDQAYELNAVGFSYLAGRYEEETKEESFQALIKSSREICEYAKSKGNMPVTLEVFDY
                     DIDKKSLIGPVHLAKRFAQIMSDEYDNFGLLVDSSHIPMLHESLDENLLPIKDYIKHA
                     HMGNTVIKDPSYPAYGDNHPRFGFPNSENDVEELAAYLRMLMKIGYLNDKERRIVSFE
                     VKP"
BASE COUNT         4349 a         1909 c         2633 g         3728 t
ORIGIN      
        1 gtggagcagt ctggtagctc gtcgggctca taacccggag gccgtaggtt caaatcctac
       61 ctccgcaatt cctaaacctt taaatcgtat tgatttaaag gtttttttat tttattgcat
      121 ttgctatggc atttatatat aatataatca attattatca ggaataatat tagaattatc
      181 tatatattaa taaatcaaat caataactat agtagaaaag aggaaagata tgccaataaa
      241 aataccaaac gatttacctg caaaacaagt attagaaaat gagaatatct ttgtaatgga
      301 tgaaacccgt gcggttactc aggatataag agaattaaaa atagttattt tgaatttgat
      361 gcctaaaaag caggaaacgg aagttcaatt gctcaggctt ataggtaatt cacctcttca
      421 ggtagaaatt acattactgc atcccaaaac ccataaatct aaaaatactt ctgaagaata
      481 cttgctggcc ttttatcata cctttgatga tattaagcat aagaaatttg atggaatggt
      541 gatcactgga gctccagtgg aaactttatc ttttgaggaa gtgacttatt gggaagaatt
      601 aaaagaaatt atggaatgga ctaaaagtaa tgtgacttct actttgcata tttgttgggg
      661 agctcaggct ggcttatatt atcattacgg ggtgcctaaa tatcctctgc ccaagaaaat
      721 gtttggcgta tttccccatt atgttttaaa aaatgcgaag ttattacagg gttttgatga
      781 tgtattttat gttccccatt caagacatac ttatacaaga agagaagata ttgaaaaaat
      841 agaagcgctc gaattattat cggtttctga tgaatcaggg gtatacattg cagcttctaa
      901 agatggaaag caaattttta taacaggaca ttctgaatac gatcccaata ccctgaaaga
      961 tgaatatgag agagatttgt caaaaggtct ggatatagag atccctatta attattttcc
     1021 tgataatgat gtaacaaaaa atccagttgt tacctggaga agccatgcta atttactttt
     1081 ctcaaattgg ttaaactatt atgtgtatca ggtgacgcct taccacttgg attaatatca
     1141 aatattgtca taaatacctt ttgtcactta tttttttatt gaaatttatt tttaaaataa
     1201 gtatactaaa tttagaatga taaatttgtg acaaattatt tttttcattg gcaactgcta
     1261 aagtgagcat aggcttttat ttgaattggg gatggagtga ataagagtga aatttgcagg
     1321 attaaaagaa tatgtttatc atgaaatcaa gcagcggtta attaataatg agataaaacc
     1381 tggagaaagg atatgggaag aggaaattgc agcagagttg gaggtaagca gaactccagt
     1441 acgggaagca attaaccgtc tgattgcaga agaatttgtt gaaaacagac ctcggaaggg
     1501 tatttttgct gctgagatat ccaaagaaga actaagaaaa atgctggatg tcaggattgc
     1561 cctggaatct ctttcagtca gtcaatgctg caagctgatt agcgaagaag aaaaagatga
     1621 attacgagca atatttgggg actacaaaag gaaaatggaa gaaggagaat atgaaaaggc
     1681 aagtcagtta gacagcaaaa tgcacagatt cattgccaaa attgccgata ataaaaaatt
     1741 agaggcctat attaatgata ttcaggattt ttttgcttat agcaggcctt atacggtaaa
     1801 atggacagaa gctaagatta aaagagccat taatgaccat aaagaattga ttgatgcttt
     1861 atgcaatgga aatgaagaaa gagcaataga aataataaga gaagatatag aagctatgag
     1921 agatttacta aaagataata atgaataagt aagttaaaaa atcaacagaa gattattcaa
     1981 agaagccact gaatagctaa gatattctca taactattca gtggcttttt ttattggatt
     2041 aagcgacaaa attctccaaa ataattgtaa tggtttaatt ttataatgtt tttgataaaa
     2101 atatataaaa agcacttgca aaaagtttaa tctgttgtat aattagatta agattataaa
     2161 ttataattta taatttataa aagattttat gagtaaggtg gaaatcggat gaaattttca
     2221 gggttaaagg aatatgctta tcacaaaatc cgggaaaaac taatcagtaa cgagatcaaa
     2281 cctggtgaaa gaatttggga agaagaaata gcagcagaat taggaataag cagaacgccg
     2341 gtacgtgaag caatcaacag attagttgca gagggactgg tgggaaacag gccacgtaaa
     2401 ggtgtttttg cagcagagat atcaaaaaac gaactaagaa aaatgctcag tgtcagaaca
     2461 gttttggaaa ctctttcggt tagtcagtgc tgtaaattga ttacagaagc agaaatgtat
     2521 gaactgaaaa aaatatatga caactacaaa agcgcaatgg aaaagggaga ctatgaaaaa
     2581 gccagtcaac tggatagtca gatacacaga tatattgcta agatttccgg aaacagacga
     2641 cttgaaaatt atattaatga cattcaggat ttttttgctt atgccagacc tcataaggta
     2701 aagtggacag aaactaaaat aaaaagatct cttcaagacc acaagaaact ggttgatgcc
     2761 atatgtaaca gagatgaaga cagtgcaata gccattatta aagaagatat agcagcaatg
     2821 gacgatttgc taaaaaatag ttaataaata agtgagtgat aggagagata ggaaatgaaa
     2881 ttatgttatc aagtagcaac accggatgta gccattgccg attcggttac tgcttaccag
     2941 ggtcccttag aaaagagttt ttatgactta gcttcttatg gttatgacgg tgttgaatta
     3001 atgaccttga accctgacga actcgactgg gactttgtaa aaaaagaggc tgaaaaaaat
     3061 aaattagcag tcgttctggt ttgtaccggg gaaatatttg gacaactggg actaagctac
     3121 acaaatccaa aagaagaaat aagaaaagaa gccattagac gaacaaaagg gattattgat
     3181 tttgcggcct ttttaggcgc taacatcaat attggcagaa ttcgcggcca gtattgtaaa
     3241 gagctttcta aagagcaaac agaaaaatat gcgatagatg catttaaaga attaagtgac
     3301 tacagtggaa agaaaaatgt acaaattgcc ttagaatctg taaccatcat gcagaccaat
     3361 ttcatcaata ccttagaaga agccgttctg atgataaaag ctgttgataa tccttatttt
     3421 aaactgatga tggacgtttt ccatttaaat ctggaagaaa aagatatttg tgaagccatc
     3481 agaaattata gtccctataa tatccatgtt catttagcgg ataataacag acgttatcct
     3541 ggtcattgcg gcttagactt tgaaaaaatc ataaagacct ttaaagaatg cggttatgat
     3601 ggaaactact gtactgaaat cttccagatc cccagtcagg aagaagctgc aaaaggtgca
     3661 atccaacatc taagacccat attcaacaag gtttatggga aatagtatag aagggagcgt
     3721 attttcatga ccaaagaaag aaaaaaagag cttcaggatt tatgcttaaa attcagacat
     3781 gatttaatag atattttaca cagtattcaa accggtcatc ccggaggttc cttatcttgt
     3841 actgaaatcg ttacaaccct ttactttgaa aaaatgaata tcgatcccaa agaccctcaa
     3901 aaagaaggaa gagaccgatt tattctctca aaagggcatg catgtccaat gctttactta
     3961 aatttagcag aaagaggatt tttcccaaag gaagacttaa aacatttaag acaaacagac
     4021 agtcacctgc aaggtcatcc ttgttttcat aaaactcctg gcgttgaatt gtcaacagga
     4081 cctttaggga tgggtcttag cgcaggtctg ggaatggcat taggggaaaa attaaaaaag
     4141 actgatgcta agatttatgt cctgttaggg gatggggaaa tacaagaagg tggaatctgg
     4201 gaagccgcta tggcagcttc aaaatttaaa gccgataatc tgattgctat tttagactat
     4261 aacggggtac agttagacgg tacattagaa gaaattatgc ctctggggga tgttgcttcc
     4321 aaatttgaat cgtttggctg gaatgttctt cactgtgacg gtcatgatgt ggaaagtgta
     4381 tctgctgcga tagataaggc aaaagaatct acggataaac cttcaatgat tatcgcaagg
     4441 acagtaaaag gaaaaggcat atcctttatg gaaggcaaaa acacatggca tggaaaacca
     4501 attagcgatg aagaatataa aatggcaaaa gccgagctgg gaggtggaaa ctaatgtcaa
     4561 agcagattgc cattcgggat gcatatggag aagctttact ggaattaggt aaaaagaatg
     4621 aaaaaatagt tgctttagaa gcagatgttg gtgcctccac aaaaagtatt ctctttggtg
     4681 aggtgtttcc tgacagatat tttaatgtag gtatcagtga gtttaatatg gttgccatgg
     4741 cagcaggttt tgcacaggaa ggttttatac cttttgttaa tacttttgct gtttttatga
     4801 ctacgagagc ttcagatcca atccaaagct taattgccta tgacaaatta aatgttaaaa
     4861 tggcaggaac ttatgcagga ctttctgact cctatgatgg tgcaagccac caggcaatta
     4921 ccgatatcgc atttgtaagg tctataccca atatgactgt gatttccgta tctgatgcgg
     4981 ttgaaacaaa aaaggcagtt gaagctattg ctgagtatga tggaccagta tatttaagat
     5041 taagccgtgc gccagcacct gtaatctttg atgaaaatta cgattttcaa ataggaaaag
     5101 gtgttgagtt acgaactgga aaagatgttg ccatcattgc gacaggcacg attttacaca
     5161 atgccttaaa ggctgcagag ttattagaag aagaaggtat cgaagcaact gtcatcaata
     5221 ttcatacgat taaacctatt gacgaagaaa tcattattaa tgctgcaaga cttacgggtg
     5281 ctgttcttac agtagaagaa cacagtattt atggtggctt aggcagtgct gtagcagaag
     5341 tactgtctca aaattatcca acacctgtag attttgtagg agcgactact tttgcagaat
     5401 caggagacta tgacgagtta ctcgaaaaat acggttatgg agccaggact atagctgaca
     5461 aagcaaaaca actgattaat agaaaataga gtataaaaag caaaatggag gaatgaagaa
     5521 atgagagaat ctattcacaa gtattttcag ataggaacca tccagtttat gagttatcca
     5581 gggattgatc ccgtggaatc cattaaaaag attgcttgtg atgactactt tgatgccatc
     5641 gaaattacca aagtagacgg cgaagaaaaa agagcacaag ttaaaaaaat gctgatagaa
     5701 tctggactta aagtgtgtta tggtgcacaa cctcttttac ttggaacaaa attaaatccc
     5761 aatgcaattg atgaagaaga aagaaagaaa gcagaagaac tgcttcttaa agctattgac
     5821 gatgcagaat actttggggc aaaaggtatt gctttcttag caggaaaatg ggaaaaagaa
     5881 acaaaagacc aggcttatga acaattgtta aagacaacaa gaaatttatg tgactatgcg
     5941 gcaacaaagg gcatgcatgt aaatctggaa gttttcgact ttgatatgga taaggctgca
     6001 ttaatcggac cagctccata cgcagcaaaa tttgctgctg acatgagaac aacacacagc
     6061 aattttggcc ttatggtaga tctttctcac ttcccgacaa cttatgaaac agccagattt
     6121 gttattcgca ccttaagacc atatattgca cacttccata ttggtaatgc cgttgtaaaa
     6181 gaaggttgtg aagcatacgg agacatgcat ccaagatttg gattccccaa tagtgctaac
     6241 aacattgaag aattattaga attctttaga gtattaaaag aagaaggttt cctggattca
     6301 aaagaaccct atgtactttc ttttgaagta aaaccatgga aagatgaaga tcctgatatc
     6361 atcttagcaa atactaagag agtgattaac agagcctggg ctcttttaga agactaaagc
     6421 tttgtcattt atttataatt tatcaacaga aaggaacggt gtgaaattga aaatagttat
     6481 tttagatgga tatacagaaa atcctggaga tttatcctgg gaaggctttg aaaagttagg
     6541 tgaattaacg gtttatgaca gaacaccagc tgataaaatt attgaaagga ttggggatgc
     6601 tgatgtggtt tataccaata aaacccccat tagcagagaa actttagatg catgtcccaa
     6661 tgttaagttt attggagtgc ttgctacagg ttataacgta gttgatgtgg aagcagctaa
     6721 ggaaaaaggc attcctgtat ccaatattcc aacctatgga accgatgctg tagcacaatt
     6781 taccattgct ttattattag aattatgcca tcatatcggg gcacattctg atgcggttaa
     6841 aaatggtgaa tggaccaata atccagactg gtgcttttgg aaatatcctt taatagaact
     6901 ggcaggaaag accatgggta ttataggttt tggaagaatt ggccaaagaa ctgcagaaat
     6961 tgcacaggct ttaggaatga aaatcttagc ttatgatgct tataagaatt cggcattaga
     7021 atctgaaacc tgcaagtatg cagaattaga tgagctcctt gcaaattctg acgttatttc
     7081 ccttcactgc cctttattcc ctgaaactca ggggattatc aataaggata ccatagcaaa
     7141 aatgaaagat ggggtaatga ttcttaacac ttcaagaggt ccattgattg ttgaagaaga
     7201 tttaagagat gccttaaaca gtggaaaggt tgccggagca gcattggatg ttgtatcaaa
     7261 agagccgatc gaaatgaata accccttact ttctgcaaaa aatatcatca ttacaccaca
     7321 cattgcctgg gctcctaaag aatccagaca aagattaatg gacattgctg ttgacaactt
     7381 aaaagctttt atttcaggca atcctcaaaa tgttgtcaac aagtaactct tttatcatag
     7441 atttcacttt tcaagcttta tgctttgaaa agtgaagtct aataagatag attttacatt
     7501 ctaaatatag gaggattgac atgacaaagt ttaaagtgaa gaatctgata ccgcttttat
     7561 ttgtggtgat ggggttagta tttgcaattt taggctttac acaacttgga ttctggagtg
     7621 atgttgatgg acccaaacca ggattctttc catcaatcat tgcgattgtt atggttttta
     7681 caggtttaat agcctttatt caatctttta aggaagaaga taaggcacaa tataatgcac
     7741 aagaatttct tgctatggca ggaggtcttg gaatttttgc agctaccttt attatcggtt
     7801 tagttccaac aattattctc tatattattc tttggttaaa attagttgaa aaagctagtt
     7861 ggaaagtgat tttaattgta ttggctatag cttgttttat taccattggg gtatttggaa
     7921 tctggttggg tattcagttt ccaaaaggca tctttgaatt cattttataa gaagggggaa
     7981 gagaagaaat gtcaaacttt gagatgttgt tacgaggatt ttcagttgta tttacaccag
     8041 ctaatatcgg ggcatccatt ttaggtgcga tattaggtct tatcgtcgga gcgatgcctg
     8101 ggattggtag tttggcagga gtagcattat tacttcccct gacatataaa tttaatccaa
     8161 caacggcaat tattatgttg ggtgcactct actattcaaa tatgtacggc ggaagcttta
     8221 gtgctatctt actgaatata ccaggagatt caccagctgt tatgactgca gttgatggat
     8281 atccgatggc taagaaaaag aaaagacctg gacaagcttt gtttacagca aatatggcat
     8341 cctgtattgg tggtaccatt ggaatgatta ttcttacctt tgcagggcca gctttagcaa
     8401 acatcggatt aaaatttgga ccatctgaaa tgacagcact tttacttgtt gcaatgactt
     8461 ctattagttg gttagttggt gaaaatccaa tcaaagggat tgtggctaca gcattcggta
     8521 ttttgttagc ctctatgggt atggatacct tatccggatc accaagatat gattttggaa
     8581 gtatgcattt attgggtgga attccattta cgccttttgt tattggttct gttggttttg
     8641 cacaagtgat taatttaatg aatgaaagat atgttcaaac agaaatgata gatgcaaaac
     8701 tttcaataag aggaagttta ccaactaaac gggaattctt aaggctgata cctccttcta
     8761 taagatctgg tattttggga acttttgtag gtgtattacc aggtgcaggg gctacgacag
     8821 gtgcatttat gggatatgcg attcagaaga attttaagag tgatgagcct ttaggaacag
     8881 gtgcagtaga aggtattgca gcttgtgaag cagcaaataa cgcagcagct gcaggttctt
     8941 ttgctccttt acttgctctt ggtattcctg gttctggaac aggtgcggtt cttcttggag
     9001 gcttaatgtt atggggtctt aatcctggac cattgctttt ccaaaatgaa ccagattttg
     9061 catggggttt aatatcatcc ctattcttat ccaatgcgat aaccctttta attgcgattg
     9121 cggttattcc attcttaacg aagatattag ctgtacctac aaaatatatg ataccaatta
     9181 ttacagtagt ttgtgtggta ggttcatata gtacaaccta ttcaatgttt ggtgtattag
     9241 taatgctttt atcaggaata gtaagctact ttattcaaaa gaataactat agtacagcac
     9301 cgatgctttt atctttcgta ttagcgccac tgcttgagtc taatatgaga aaagcattta
     9361 ttatctctgg tggtagctta aaaattttct ttacaagacc tatatctcta atgttcatgt
     9421 tgatcttctt tacaatgctt ttaattccac ttgtaagaag tatattaggt aaaaagaaag
     9481 aagaaaaaac tgcttaattt aattatttta gctcgctata taattttcat taattatata
     9541 gatataaatt ttaaatttta aatattaaga ggaggattta gtatgaagaa taaaatgatg
     9601 gctttattgg caggggtttt aacagtatct gtacttttta caggttgcca aaaatctggc
     9661 ggaaattcca gttcaggtgg aacaagctca ggcggaacaa gttctggaga aaaaactact
     9721 gtaactaaag aatttaaacc agcttcaacg gttgactggt atgttgctag tagtccaggt
     9781 ggtggcagcg acattttcac cagaatgatt acagacatta tgatttctga aggaattgtt
     9841 gacacaaact ttattgttac caacaagacc gacggtggcg gtgaagttgt tcgtaatgaa
     9901 gtagcacata tgtcgaaagg tgacagagca aacaatactt tattaacttt taacagcgga
     9961 tgtttaatgc caatggttga aaacactaaa aatcgttcca gcaactttaa agttatcgct
    10021 gttatggcag tagacaaaca attacttttc aaaacagaac atacaaaata tgcagatttt
    10081 aatgaagcaa ttgaagcagc aaaaagtggt gcaagcattg ttattggtgg ttcaaaaggt
    10141 gacgacgttg caacttatga agcattaatt caagaaattg gtgtttctgc agacaacatg
    10201 aagtacatta cttacgatgc aacttctgac gctattactg caggcttggg cggacacgtt
    10261 gactttgtta tgtctaaacc agcagcagca tctgaatatg ttgaagcagg ttcattaatt
    10321 cctgtattgg cactttcaaa agaaagattt tcaggtaatt tagctgatgc tccaacatta
    10381 agtgaaattg gagattatga agatgttgaa gttcctgtat ggcgtagtgt tgtagcacct
    10441 gccagcatga gtgatgaagc ggtagcattc tggaccgatg ctcttaaaaa agtatctgaa
    10501 acagaaaaat ggcaaaatga ttatattgca aaatataaat taattcctga atacatggat
    10561 gcagcaacag caactggcta tatcagtgaa ttcgaaaaga atttcatggc atctaaagga
    10621 attcaataat ttaagaagtt aaaaactgca acaatcacca acaagttaaa aagaagtgct
    10681 gtctaaatac tatgatagta cttcttttta acaaagactt aaaaggaaac aggattaaaa
    10741 tagatttatc atctgggagg aaaatatatg gagaagaaac cgattattgg gataatttta
    10801 ggggatgcag caggcgttgg tccagaaatt atcgcaaaac tggcagcaag aaaattttac
    10861 gatgaatatt gcagacccgt gattttaggg gatgcaagag tttttgaaga aggtgcaaga
    10921 gtagcaggtg ttgaagttcc cgtacaaatt attgaagacg ttgatgatgc aacatgggaa
    10981 aaaggtattc ctgttcttga tcaaaaagat ttagaccctg caaacattcc ttatggacaa
    11041 cttaatgttg aaagtgggaa agcttgtctg gatttattaa aattagctgt tgaactgttt
    11101 caggctgaaa agattgatgg cttttgcttt ggtcctttaa ataaatccgc tatgattgct
    11161 gctggttgtc cttacgaaag tgaacatcat tacatggcag cgttgtttaa tcatacggaa
    11221 ccctttggag aaattaacta cttagatggt ctttggacga caagaactac cagtcacatt
    11281 ccaattaagg aagtaagcaa tcatttaacc gtagacagaa tcttaagagc tgtccgttta
    11341 gccaatacaa ctttaaaaaa tgccggatat gataaacctg ttttaggtct ggcggctctt
    11401 aacccacact gtggagaaaa tggtaaatgc ggcagagaag aaattgatgt gatagccccg
    11461 gctattgaaa aagcaattga aatgggaatt gatgcaagag gaccatatcc tgcagatatt
    11521 ctttttatta aagcatttaa cggggaattt gacggtgtgg taacaatgta tcatgaccag
    11581 ggtcaaattg cattaaaact taaaggattc gactatggca ttaccattgc aggtggtctt
    11641 ccagctccta tcgtaacttg tgcgcatgga accgcttatg acattgcagg aaaaggcatt
    11701 gcaaaaacaa ctgcttttga aaatgcagta aaaatggcag ccagaatggc ttctcatatc
    11761 cgcggctaag aaaggagttt attatgaacc tttcaatgag aaaatatatg aaggtaggta
    11821 ttatccttca tatggctttt gatggtctta tttccggaga aggtcctatt ttggaatgtt
    11881 tagaaaaaat agctgctgac gagtattttg aagttgtaga aattacacaa atcaaagatc
    11941 ctggagttag aaaagctgta agggaaatgg ttgaaactgc tcatatggaa ttgatttatg
    12001 gcggacaacc aaggctgctt acaaaaggac ttaacattaa tgatttagat gaagaaaaaa
    12061 gaagattagc cgtagaagaa ttaaaagacg gcattgatca ggcttatgaa ttaaatgctg
    12121 taggattttc ctatttggca ggcagatatg aagaagagac taaagaagag tctttccagg
    12181 ctttaatcaa atccagcaga gaaatttgtg aatatgcaaa atcaaaaggt aatatgccag
    12241 tgacattaga agtatttgac tatgatattg ataaaaaatc tttaattgga cctgtgcatt
    12301 tggcaaagag atttgctcaa atcatgtctg atgaatatga taattttggt ttgttggttg
    12361 actccagtca tattcccatg cttcatgaaa gcttagatga aaacttactc cccattaaag
    12421 attatataaa acatgcccat atgggtaata cagtcattaa agatccctct tatcctgctt
    12481 acggggataa tcatccacgc tttggtttcc ccaatagcga aaatgatgtg gaagaattag
    12541 cagcttattt aagaatgctg atgaaaatag gttatttaaa tgataaagaa cgccggattg
    12601 taagttttga agtaaagcc
//
LOCUS       DAOSUD010000061        12614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2919_length_12614_cov_22.949200, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000061 DAOSUD010000000
VERSION     DAOSUD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_12614_cov_22.949200"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1187
                     /gene="hrcA"
                     /locus_tag="PLG49_08130"
     CDS             141..1187
                     /gene="hrcA"
                     /locus_tag="PLG49_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOQ17323.1"
                     /translation="MDFEMNLDERKIKILKAIIKDYILTGEPVGSRTISKKYELGVSS
                     ATIRNEMSDLEEWGFIIQPHTSAGRIPSDKGYRLYVDKLMEYQAIEDDQLRLFERTLE
                     QKIDQIDELMMEVAKLISYFTNYTSMVSSPQIQKTKLKHMQLIPLDKRSVMLIIVTNA
                     NIVTNNVIRMDVPADQDTLNILSNMLNESLRGLTIEEINLPLIQSLKLKMGIYGEIVN
                     SILDAIDHTIQRKNTPDIYLSGTKNLLNFPEFGDLVKTKQIFHTLEEKELLASLLSKA
                     ANDGIKIIIGQENEIDEMKHCSIITASYEIDGKTVGTIGVIGPTRMEYSKAVSILSYI
                     SRNLDKLLKKISDG"
     gene            1215..1877
                     /gene="grpE"
                     /locus_tag="PLG49_08135"
     CDS             1215..1877
                     /gene="grpE"
                     /locus_tag="PLG49_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ17324.1"
                     /translation="MEERQKNDHFDQEEIKDEDIIEDTEVLNEDTKDNEKNDDSKPSN
                     EDLEEASVESAEASDEELKEETDIDEMKKLLQEKENQCADLLDRLQRTMAEFDNFRKR
                     TIKEKTSLFDDGVRSTIEQILPVIDNFERALSSNTTEDESNPYLQGMEMIYRQFKDIL
                     AAIGVEEIKTIGEKFNPDFHNAVTHIEDEAYGENEIVEEFQKGYIYKDKVIRYSMVKV
                     AN"
     gene            1912..3765
                     /gene="dnaK"
                     /locus_tag="PLG49_08140"
     CDS             1912..3765
                     /gene="dnaK"
                     /locus_tag="PLG49_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOQ17325.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPVVIPNSEGARTTPSIVAFSK
                     NGERLIGEPAKRQAVTNPEKTIMSIKRHMGSDYRVNINGKSYSPQEISAMILQKLKAD
                     AEAYLGETVTEAVITVPAYFSDSQRQATKDAGRIAGLEVKRIINEPTAAALAYGLENE
                     KEQKIMVYDLGGGTFDVSIIEIGDGVIEVLATNGNNRLGGDDFDERIIQELIQDFKNK
                     EGIDLSQDKMAMQRLKEAAEKAKKELSTVTTTNINLPFITANQEGPKHLDMTLTRARF
                     EELTADLVEKTMEPVRLALSDAGLEASDLDKVILVGGSTRIPAVQEAVKRLTGKEPFK
                     GINPDECVAIGASIQGGKLSGDEGAGDILLLDVTPLSLGIETLGGVTTRLIERNTTIP
                     TRKSQIFSTAEDNQTAVDIHVVQGERQMAKDNKTLGRFRLDGIPSAPRGVPQIEVTFD
                     IDANGIVNVSAKDLGTGKEQKITITASTNLSEDEIERAIKEAEQFAEQDKKKKEAIDT
                     KNEADSLVFQTEKTLKELGDKIEAADKTKIEDELKKVKELLQKFDTENINESQVAELK
                     KAKESLTNIFYEISAKLYQQQGPQGGANASSGPEKKNDGPDDVVDADYKEI"
     gene            3934..5094
                     /gene="dnaJ"
                     /locus_tag="PLG49_08145"
     CDS             3934..5094
                     /gene="dnaJ"
                     /locus_tag="PLG49_08145"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ17326.1"
                     /translation="MTERDYYEILGVDKNASETDIKRAYRKLAKKYHPDANPGDKEAE
                     QKFKEINEAYEVLSDPQKREKYDQFGHAAFSNGAAGAGGFDGFGGFSGFGGFGMDDIF
                     DMFDIFGSRSRRSSSQPTKGENIRMSMELSFHEAAFGTSKELQINVWDTCDTCGGSRV
                     KKGSHPETCKKCGGTGQVRYTQTTFLGTVSRVQTCDNCHGEGIIIKDPCPACNGKGKV
                     RKTKTIQVDIPAGIDHGQSIRIQGKGEPGERGGPNGDLLITVYIREHPIFERRGNDVY
                     CEIPITFVQAALGGELVVPTLDGNVQYTIKEGTQTGTVFRLRGKGIPHLGNPKIRGDQ
                     YVEVKVEVPTKLNEAQKKILQEFANISGEEIYQQRKGFFKKVKDAFGDAFGN"
     gene            5106..6074
                     /gene="prmA"
                     /locus_tag="PLG49_08150"
     CDS             5106..6074
                     /gene="prmA"
                     /locus_tag="PLG49_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOQ17327.1"
                     /translation="MYNLDWIRIIIKTNSQGQEAIGYALSQLGIQAVEIEDPFEIITA
                     IEKKKEETQVQWDYIDEELIQNNKDEVLIKAYLAEDTPYEDKIIQIQEKINHIKSFLD
                     IGSGEIEIEKVNEEDWAESWKKYYKPFRLGKHIIIKPIWEEYSSAEKNDIIVEMDPGM
                     AFGTGTHETTSMCIELLEKYLNENACVFDIGCGSGILSIVAAKLGADHVIAVDLDSNA
                     VEASKKNVALNQLQEKVRIFQGNLLDNISEKADIVVANIIADVIIQLSNSVPDYLKNE
                     ALFIASGIIKERKDEVISVLERNGFNIIEITEKGEWCALVAQKRSE"
     gene            6077..6814
                     /locus_tag="PLG49_08155"
     CDS             6077..6814
                     /locus_tag="PLG49_08155"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOQ17328.1"
                     /translation="MLPRFFVSPSQIQGKQIVIQGEDVKHISKVLRLKKGDKILICNR
                     QGIDYECIIKDMEKIITAEIISKNASIGEALTKITLFQALTKSDKMDYIIQKAVELGV
                     HRIVPVVTERTIVKIENEKKENSKLQRWNKISESAAKQSQRGIIPEVAAVIPLIEAFN
                     QAEKMDLKLIPYEMEKNNHLKDLLANCTAKSIAIFIGPEGGFEESEINLAKEHGIIPV
                     TLGRRILRTETAGLVTVSIMMYEMGEM"
     gene            6817..7185
                     /locus_tag="PLG49_08160"
     CDS             6817..7185
                     /locus_tag="PLG49_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ17329.1"
                     /translation="MAKLRFLVVDDAIFMRTVLKKMLTEEGFEVVGEAGNGLEAIQMA
                     AKLQPDIITLDITMPEMNGIKAVPEILKVSPKSKIIICSALGQQAMVVDAIRKGAKDF
                     IVKPFEKSRVLQAIHNVSRR"
     gene            7276..8442
                     /locus_tag="PLG49_08165"
     CDS             7276..8442
                     /locus_tag="PLG49_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOQ17330.1"
                     /translation="MEEIYFDNAATSQPSAMVIEKMTQVLSKDYGNPSSLHLKGLEAE
                     RHIRNTSQILSSLLKVGEDEIIYTSGGTESNNLAIIGIAQSYHRRGKHIITSKIEHPS
                     VYYAMKYLEEEGFEISYLENDSKGYIDLNQLKKEIREDTILVSIMHVNNEIGTIQPIA
                     EIGEIIKKINPGTVFHVDGVQSFGKLLLMPKKWNIDLLSISAHKFHGPKGIGALYKNK
                     KVRLKSLLYGGGQQKGLRSGTENVPGIVGLGIAAEEIYQELDDNNRHILSLKKMLWEG
                     LSSHIDDVYLNGPDLEDGAPNIINILFKNIKGEVLLHALEDKKIYVSTGSACSSNQPH
                     LSQTLKSLGLSDEEVQSSLRFSFSKYNTEEEVKNCIQALKDIVPILRRFKAGGR"
     gene            8443..9621
                     /gene="thiI"
                     /locus_tag="PLG49_08170"
     CDS             8443..9621
                     /gene="thiI"
                     /locus_tag="PLG49_08170"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOQ17331.1"
                     /translation="MKNIYLVKYGEIAIKGKNRYLFEDALLSNIRSSIKKLGKFHIKK
                     EQGRILIEPEDKDVDKDEVIEQLKKVFGIVAICPGIKNEEISMDSIKETALYYMKNIC
                     QKNQYTFKVETRRSDKRFPLNSMEISRDVGAYLLKNMNGQLSVDVHSPQIRLNIELRN
                     SVYVYSMVIPGPGGMPVGTNGKAMLLLSGGIDSPVAGWMIAKRGVEIGAVYFESPPYT
                     SERAKQKVVDLARTIATYTGKMKLFVVPFTKIQMEIYEKCPHDQLTIIMRRMMMKIAE
                     RLAKEENALGLITGESIGQVASQTIQSLAVTNAAVELPVFRPLIGFDKEEIVQIAKKI
                     NTYEISILPYEDCCTIFVPKHPETKPKLEYILQSEKSLDNMESLLEEAIANKEIIMIE
                     "
     gene            complement(9644..9877)
                     /locus_tag="PLG49_08175"
     CDS             complement(9644..9877)
                     /locus_tag="PLG49_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ17332.1"
                     /translation="MKSITISLNSIEKVKQFVNTIGKYNGDFDLSSGRYKVDAKSIMG
                     IFSLDLSAPLVLDIHDDSIADEVIQAIKDYIIK"
     gene            9988..11292
                     /gene="mtaB"
                     /locus_tag="PLG49_08180"
     CDS             9988..11292
                     /gene="mtaB"
                     /locus_tag="PLG49_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOQ17333.1"
                     /translation="MATAAFYTLGCKVNQYETEALIELFKDRGYEIVDFGDYADVYVI
                     NTCTVTNLGDRKSRQMIRKAKKMNPSSIVAVVGCYVQVAPEKVKAIEEVNIIIGTDER
                     HRIAELIEQYKEEQKNISLVKNIMKVKEFEELSVSKLEGRTRAYLKIQEGCNQFCSYC
                     IIPYARGPVRSRKPENIINEVNKLVYNGFKEIVLTGIHVASYGKDLGNINLLKLLEMV
                     HTIKGLERIRLSSIEPSIVNEEFVNTIQGLHKICPHFHLSLQSGCDETLKRMNRKYTT
                     KDYKEAVELLRDKIEDIALTTDIIVGFPGENDEEFNQSYHFVKEIAFSQIHVFKFSPR
                     QGTPAARMKDQIAPEVKEERSRKMIELGQFLQNQFLVQQIGKTMDVLFENTLDDHEEI
                     FEGHTPNYLKVEVHSKEDIKNQILPVTFQGIKNHSLIGNIKN"
     gene            11398..11652
                     /locus_tag="PLG49_08185"
     CDS             11398..11652
                     /locus_tag="PLG49_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOQ17334.1"
                     /translation="MSEFNETVQFNVDKDKGNEIRDILFYVYEALKEKGYNPVNQIVG
                     YILSGDPTYITSYNNARSIIRKLERDELLEELIKSYLEKN"
     gene            11667..>12614
                     /locus_tag="PLG49_08190"
     CDS             11667..>12614
                     /locus_tag="PLG49_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17335.1"
                     /translation="MFKRIFLTITVLIFFFMADKSYGESTKSMLLIVTESSWDAFLAS
                     PFISSFIPESSVGSMNIRTNHQNKIIEYYGTINAGRRWSYYEVNDMGAVDKGLGDYLN
                     QSGLSTAFFSDKDELIKIIENQYGMTSYRTNDFEKLFSDDNKKILETADVILIDMDLY
                     CTKENQDFLLDFIKKRHVRLYVFCPYHVENKLTPFLFYDSRKPYPGLITSATTRRQGI
                     ITNLDIAPSILSYYGIDCSSMVSKGIEIQYIPDAYKKLNHLLEKIEFLNYYRSNIIKI
                     YNFFLISLLIIYFFLEKRNHYMFNIIYDAFIIFALWLPVL"
BASE COUNT         4659 a         1677 c         2584 g         3694 t
ORIGIN      
        1 atttagccaa tatcgtattt gcagaatttt taccataaat aattaaataa tatcttgaca
       61 aatgttgttt tgaatggtat tttatttgtg aagttagcac tcgagctaat agagtgctaa
      121 caactaaggg gggaaacctg atggattttg aaatgaattt agatgaaaga aaaattaaga
      181 tattaaaagc aatcattaaa gattacatac ttactgggga acctgtaggt tccagaacga
      241 tttctaaaaa atatgaattg ggtgtaagtt ctgcgacgat tagaaacgaa atgtctgatt
      301 tagaagaatg gggttttatt attcaacctc atacttctgc tggccgaata ccttctgaca
      361 aaggttatcg cttgtatgta gataaattga tggaatatca agcgatagaa gatgatcagc
      421 ttagattatt tgaaagaaca ttagaacaaa aaattgatca aatcgatgaa ttaatgatgg
      481 aagttgctaa attgatttct tattttacga actatacttc catggtatca tctcctcaaa
      541 ttcagaaaac aaaattaaaa catatgcagt tgatcccctt agataaaaga tcagttatgt
      601 taattattgt gaccaatgcc aatattgtta caaacaatgt gatcagaatg gatgtgcctg
      661 ctgatcagga tacacttaac attttatcca atatgcttaa tgaatcttta agaggactta
      721 ccattgaaga gattaatctt ccattaatac aaagtttaaa attaaaaatg ggtatctatg
      781 gagaaattgt gaattcaatt cttgatgcca ttgatcatac tattcaaagg aaaaatacac
      841 cagatatata tctgagtggt accaagaatt tattaaactt ccctgagttt ggagatttag
      901 taaaaacaaa acagattttt cataccttag aagaaaaaga actgttagct tcccttttat
      961 ctaaagcagc caatgatggc attaaaatca ttattggcca ggaaaacgaa atcgatgaaa
     1021 tgaaacattg cagtattatt actgcttcat atgaaattga tggaaaaacc gtaggaacga
     1081 ttggtgtcat aggtcctacg agaatggaat atagtaaagc tgtttcaatt ttgtcttata
     1141 tttctagaaa tttagataag ttgcttaaaa aaatatctga tggataatat cataagaaga
     1201 aaagggtgtg tcaagtggaa gaaagacaaa aaaatgatca tttcgatcag gaagaaataa
     1261 aagatgaaga tatcatagaa gatacagaag tcttaaatga agatacgaaa gataatgaaa
     1321 aaaatgacga cagcaagccg tccaacgaag atttagaaga agcttctgta gaatcagcag
     1381 aagcctcaga tgaagagctg aaggaagaaa ctgacataga cgaaatgaaa aaattgcttc
     1441 aggaaaaaga aaatcaatgc gccgatttgt tagacagact gcaaaggact atggctgagt
     1501 ttgataactt cagaaaacgg acaataaaag aaaaaaccag tttgtttgat gatggtgtca
     1561 ggagcactat cgagcaaata ctgccagtta tagataattt tgaaagagct ctgtcttcca
     1621 atactacgga agatgaaagt aatccttatc ttcaaggcat ggaaatgatt tacagacaat
     1681 ttaaagatat tttagctgcc ataggcgtag aagagataaa aacgataggg gaaaaattta
     1741 accccgattt tcacaatgcg gtgacgcata ttgaagatga agcctatgga gaaaatgaaa
     1801 ttgttgaaga atttcaaaaa ggatacatct ataaggataa agttatccga tacagtatgg
     1861 ttaaagtggc caattaataa agacaacgaa ttgatttaag gaggaaataa tatgggtaaa
     1921 attataggta tcgatttagg tacaactaat tcatgtgtag ccgttatgga aggcggaaaa
     1981 cctgttgtta ttccaaattc agaaggtgca agaacgaccc cttccattgt tgcttttagt
     2041 aaaaacggag aaagattaat tggtgaacct gcaaaacgtc aggcggttac aaatcctgaa
     2101 aaaaccatta tgtctattaa acgacatatg ggttcagatt atagagtgaa tattaatggc
     2161 aagtcttaca gcccacaaga aatttcagca atgattttac aaaaattaaa ggcagatgca
     2221 gaagcttatt taggagaaac tgtaactgaa gcagtcatta cagtacctgc ttatttttca
     2281 gatagtcaaa gacaagcaac aaaagacgct ggaagaattg ctggtctgga agtaaagaga
     2341 attattaatg agcctactgc agcggcatta gcttatggac ttgaaaatga aaaagagcaa
     2401 aaaattatgg tatatgattt gggtggcgga acttttgacg tttctattat tgaaatagga
     2461 gatggcgtaa tagaagttct tgcaaccaat ggtaacaacc gtcttggagg agacgacttt
     2521 gatgagagaa ttattcagga actcatacaa gattttaaaa ataaagaagg aattgattta
     2581 agccaggaca aaatggcaat gcagagatta aaagaagctg ctgaaaaagc aaaaaaagaa
     2641 ctgtctacag tgacaaccac caatattaat ttgcctttca ttacagcaaa tcaggaagga
     2701 ccaaaacact tagatatgac cttaacaagg gcaagatttg aagaattaac agccgatctt
     2761 gttgaaaaaa ccatggaacc ggtaagattg gctttgtctg atgcaggttt agaagcgtca
     2821 gatttagata aagtgatttt agtaggtggt tcaacccgca tacctgctgt acaggaagca
     2881 gttaaaagac ttacgggtaa agaacctttt aaaggaatta atccggatga atgtgttgct
     2941 attggtgcat ctattcaggg tggaaaactt tccggcgatg aaggtgcagg agatattctt
     3001 ttattggatg ttacacccct ttctcttgga attgaaacat tgggcggggt aacaacccgt
     3061 ttaatagaaa gaaatacaac tattcctaca agaaagagtc agattttctc aacagctgaa
     3121 gacaatcaga cagccgtaga catccatgtc gttcagggcg aaagacaaat ggcaaaagat
     3181 aataagacct taggaagatt cagattagat ggtattccat cagctccaag gggcgtacct
     3241 caaattgaag taacttttga tattgatgcc aacggaattg ttaatgtgtc tgcaaaggat
     3301 cttggaactg gaaaagaaca aaaaattact attacagcaa gtacgaattt aagtgaagat
     3361 gaaatagaaa gagctattaa agaagcagaa caatttgcag aacaagataa gaagaaaaaa
     3421 gaagccattg atacgaaaaa tgaggcagat tctttagtat tccagactga aaaaacatta
     3481 aaagaacttg gagataaaat tgaggcagca gataaaacga agattgaaga tgaacttaaa
     3541 aaagtaaaag agcttcttca aaaatttgat acggaaaaca ttaatgaaag tcaagttgca
     3601 gagctgaaaa aagcaaaaga atctttaacc aatatttttt atgaaatttc tgcgaaacta
     3661 tatcagcaac aaggtcctca gggtggagca aatgccagca gcggaccaga aaagaaaaat
     3721 gatggtccgg atgatgtggt agatgctgat tataaagaaa tataatagcc ctaacttaaa
     3781 aagccaaaga gaaattcttt ggctttttaa aagttatatt tgttttttaa ttgcttttag
     3841 gagattaaaa atatataata tattaattga tatgaatgat tgccttaatc cgagcttgcc
     3901 caggaataat tgttatgttt ggaaggtgag ggaatgacag aaagagatta ttacgagata
     3961 ttaggggtag ataaaaatgc ttctgaaaca gacataaaga gagcctatag aaaactggca
     4021 aaaaagtatc atccggatgc caatccagga gataaggaag cagaacaaaa gtttaaagaa
     4081 ataaatgaag catatgaagt tcttagcgat cctcaaaaaa gagaaaaata tgaccaattt
     4141 ggtcatgcag ccttttctaa cggagctgcc ggagccggag gttttgatgg ttttggaggt
     4201 tttagtggtt ttggtggctt tggtatggat gatatttttg acatgtttga tatttttgga
     4261 agcaggagca gaagaagcag ttcacagccg accaagggtg agaatatccg tatgagcatg
     4321 gaattgtctt tccatgaagc tgcttttggg acttctaaag aattacagat caatgtatgg
     4381 gatacttgtg atacatgcgg gggaagcagg gttaagaagg gaagtcatcc ggaaacctgt
     4441 aaaaaatgtg gtggaactgg tcaggtaaga tatactcaga caacattttt aggtactgtg
     4501 tccagggttc agacctgtga caattgtcat ggagaaggaa tcatcataaa agatccttgt
     4561 cctgcttgta atggaaaagg taaggttaga aaaacgaaga ccatacaagt ggatataccc
     4621 gcagggattg atcatggtca aagtataaga atccagggta aaggtgagcc tggggaaaga
     4681 ggcggtccga acggagatct ccttattaca gtctatatca gagaacatcc aatatttgaa
     4741 agacggggca atgatgttta ttgtgaaatt cccattacct ttgttcaggc agcacttgga
     4801 ggagagttag tggttcctac tttagatggt aatgttcagt ataccataaa agaaggaacc
     4861 caaacgggta cagtatttag attaagagga aaaggtattc cccatctcgg aaatcctaaa
     4921 atccgcggag atcaatatgt agaagttaaa gtagaagtac cgactaaact aaatgaagca
     4981 caaaagaaaa tactgcagga atttgctaat ataagtgggg aagaaatcta tcagcaaagg
     5041 aaaggctttt ttaagaaagt aaaagatgct tttggggatg cctttggaaa ttaatttaag
     5101 aggagatgta caatttggat tggattcgta ttataataaa gacaaatagt cagggacaag
     5161 aggctatcgg ttatgcttta tctcaattgg gtatccaggc agtggaaata gaagatcctt
     5221 ttgaaattat tactgcaata gaaaagaaaa aagaagagac ccaggtccaa tgggactaca
     5281 ttgatgaaga actgattcaa aataataagg atgaagttct tataaaagct tatttggcag
     5341 aagatacacc ctacgaggat aaaataatac aaattcagga aaagataaat catataaagt
     5401 cttttctgga tattggcagt ggggaaatag aaattgaaaa ggtgaatgag gaagactggg
     5461 cagaatcatg gaaaaaatat tataagccct tcagactggg aaagcatatt attataaaac
     5521 ctatttggga agagtattca tcagctgaaa aaaatgatat aatagtagag atggatccag
     5581 gtatggcatt tggtaccggg actcatgaga cgacttctat gtgtatcgaa ttacttgaaa
     5641 aatatcttaa tgaaaatgct tgtgtttttg acataggttg tggcagtgga attttaagca
     5701 ttgttgcggc aaaacttggt gctgatcatg tcattgcagt agatttagat tctaatgctg
     5761 tcgaggcaag caaaaagaat gtagcattaa atcaacttca ggaaaaggtt agaatttttc
     5821 agggaaatct tctggataat atttctgaaa aagcagatat cgtggtggct aatattattg
     5881 cagatgttat tattcaactt tccaattcag ttcctgatta tttaaaaaat gaagctttgt
     5941 ttattgcttc aggtattata aaggaaagaa aagatgaagt aataagtgtt ttggaaagaa
     6001 atggattcaa tatcattgag ataactgaaa agggagaatg gtgtgctctt gtagctcaaa
     6061 agaggagtga ataattttgc taccaaggtt ttttgtcagt ccttctcaaa tacaaggtaa
     6121 acagattgtt attcaggggg aagatgtaaa gcatatcagc aaggttttaa gattaaaaaa
     6181 aggtgacaaa atattaattt gtaatagaca aggaatagat tatgagtgta taataaaaga
     6241 tatggaaaaa ataattaccg ctgaaattat ttccaaaaat gcttcaatag gagaagcgct
     6301 tactaaaata acattatttc aagctttaac caaatcagat aaaatggatt atattattca
     6361 aaaagcagta gagctaggtg tgcataggat agttcctgtt gttacagaaa gaaccattgt
     6421 taaaattgaa aatgagaaaa aagaaaattc caaattgcaa agatggaaca aaatttcaga
     6481 atcagcagca aagcaaagtc aaagaggaat tattcctgaa gtggcagcgg ttattccttt
     6541 aattgaggca tttaatcagg cagaaaagat ggatttaaaa ttaataccct atgaaatgga
     6601 aaaaaataat catttgaaag atttattagc taattgtact gctaaaagca ttgctatatt
     6661 tataggtcct gaaggcggat tcgaagaaag tgaaattaat cttgccaaag aacatgggat
     6721 tataccagtt actttgggaa gaagaattct tcggactgaa acggcaggac tcgtcacagt
     6781 atcaattatg atgtacgaaa tgggagagat gtaaaaatgg caaagctcag atttttagtg
     6841 gtagacgatg caattttcat gcgcactgta cttaaaaaaa tgctcacgga agagggcttt
     6901 gaagttgttg gagaagcagg taatggcttg gaagcaattc agatggccgc aaaactacag
     6961 cccgatatta ttactttaga tattaccatg ccggaaatga atggaattaa ggctgttccg
     7021 gaaatactga aagttagtcc aaaatcaaaa ataatcattt gttctgcttt aggacaacaa
     7081 gctatggttg tggacgcaat taggaaaggc gcaaaagatt ttattgtaaa accatttgaa
     7141 aaatcgagag tgctccaggc aattcataat gtttcaagaa ggtagaaact ggtaggtaat
     7201 cctaccggtt ttttttgggg tactctttgc gaatgaatag ataaaaagga ctaaaagaat
     7261 aaagaaggtg gaagaatgga agaaatatat tttgataatg cggctacaag ccagccttct
     7321 gctatggtta tagaaaaaat gactcaggta ttaagtaagg attatggaaa tccttcttcc
     7381 cttcatctga aaggattaga agcagaaagg catattagaa atacaagtca aattctttca
     7441 tcactcctaa aggttggtga agatgaaatt atctatactt caggaggaac tgaatcgaat
     7501 aatttagcca tcattggtat tgctcaaagt tatcatcgca gaggaaaaca cattattacc
     7561 agtaagattg aacatccttc cgtttattat gctatgaaat accttgaaga agaaggcttt
     7621 gaaattagtt atttagagaa tgattccaaa ggttatattg atttgaatca actgaaaaag
     7681 gaaatccgtg aagatacgat attggttagc attatgcacg ttaacaatga aattgggaca
     7741 atacagccga ttgctgaaat tggtgaaatc ataaaaaaga ttaatccagg tactgtattt
     7801 catgttgatg gggttcagtc ttttgggaaa ttattattga tgcctaaaaa atggaatata
     7861 gatttattaa gtataagtgc tcataaattc catggaccta aaggtattgg agcattgtat
     7921 aagaataaaa aggtaaggtt aaaatcactg ttatatggag ggggacagca aaaaggcctc
     7981 aggtctggta cagaaaatgt tcccggtatt gttggtttag ggattgcagc cgaggaaatt
     8041 tatcaggaac tagatgacaa taacagacat atactttccc tcaagaaaat gctttgggaa
     8101 ggactttcca gccatattga tgatgtatat cttaatggtc ctgatttgga agatggtgca
     8161 cccaatatta ttaatattct ttttaagaat attaagggag aagttttact acatgcttta
     8221 gaagataaaa agatttatgt ttctacaggt tcagcctgtt catccaatca gcctcaccta
     8281 agccagaccc tgaagtcttt aggcttgtct gatgaagagg ttcaaagttc actgaggttt
     8341 agtttttcaa agtataatac agaggaagaa gtcaaaaatt gtattcaggc attaaaagat
     8401 atcgtgccga tactcaggag atttaaagca ggaggaagat aaatgaaaaa tatttatctc
     8461 gtgaaatatg gagaaatcgc aataaaagga aagaacagat atttgtttga agatgctctt
     8521 ttatccaata ttagaagcag tatcaaaaaa cttgggaaat ttcatataaa aaaagaacaa
     8581 ggaagaattc ttattgaacc agaagataaa gatgtcgata aagatgaagt gattgagcag
     8641 ttaaaaaaag tatttggaat cgtggcgatt tgcccgggaa taaaaaatga agaaatcagt
     8701 atggattcca ttaaagaaac ggctctttat tatatgaaaa atatatgtca aaagaatcaa
     8761 tatactttta aagttgaaac aagacgttca gacaagcgct ttccattaaa ttctatggag
     8821 atatccagag atgtcggagc ttatttgctt aaaaatatga atgggcagtt atctgtggat
     8881 gttcatagtc ctcaaatacg tttaaatatt gagttaagaa attctgttta tgtttattct
     8941 atggttattc caggtcctgg cggtatgccg gttggtacca atggaaaagc tatgctttta
     9001 ttatctggcg ggattgatag tcctgtagca ggctggatga ttgccaaaag aggtgtagag
     9061 ataggagccg tttattttga aagtccacct tacacatctg aaagggctaa gcaaaaagtg
     9121 gttgatttgg caaggactat agccacgtat actggaaaaa tgaaattatt tgtcgttcct
     9181 tttactaaaa ttcaaatgga aatatatgaa aagtgtcctc atgatcaatt aacgattatt
     9241 atgagaagaa tgatgatgaa aattgctgaa aggctggcaa aagaggaaaa tgctctgggg
     9301 cttataacag gagaaagcat tggacaggtt gccagtcaga cgatacaaag tttagctgtt
     9361 accaatgctg ccgtagaatt gccagtattt cgtcctctta taggatttga taaggaagaa
     9421 atcgttcaga ttgcaaaaaa gataaacact tatgaaatat cgattttacc gtatgaagat
     9481 tgctgtacca tatttgttcc aaaacatccg gagacaaaac ccaaactgga atatatttta
     9541 caatccgaaa agagtctgga taatatggaa tctcttttag aagaggctat tgcgaataag
     9601 gaaataatta tgatagaata aataatccac tttttaaagt ggattattta atgatatagt
     9661 cttttatggc ttgaattact tcatcggcaa tactgtcatc atggatatcc aaaactaaag
     9721 gggcacttag atccaggcta aaaattccca tgatagattt tgcgtcaact ttatatcttc
     9781 cggaagataa atcaaaatcg ccattatatt ttccgatggt attaacaaat tgcttaactt
     9841 tttcaatgga atttaaagaa attgttattg atttcataga aacctccata taatatttgt
     9901 ttctaactat tgtatgatat ttatttctaa ttataatagt aagtctttaa aaaatttttg
     9961 tcaatcctgt tatgaagagg tgaaagaatg gcaacagcag ctttttatac tttaggctgt
    10021 aaggtgaatc aatatgaaac agaagcttta attgagttat ttaaagatag aggatatgaa
    10081 atagtagact ttggtgatta tgctgatgtc tatgtgatta atacttgtac agtgactaat
    10141 ttaggcgaca gaaagtcaag gcaaatgata agaaaggcca aaaaaatgaa tccttcttcc
    10201 attgttgccg ttgtaggctg ttatgtgcag gtagctcctg aaaaagtcaa agcaattgag
    10261 gaagttaata ttattattgg aacggatgag aggcatagaa ttgctgaatt aatcgaacag
    10321 tataaagaag aacaaaaaaa catcagtttg gtaaagaaca ttatgaaagt caaagaattt
    10381 gaagagttat ctgtaagtaa actggaaggc agaacccgag cttatcttaa aatccaggaa
    10441 ggttgtaatc agttttgctc ttattgtatt atcccttatg ccagaggacc tgttagaagc
    10501 agaaaacctg aaaacattat aaatgaagtg aataagctgg tttataatgg ctttaaggaa
    10561 attgttttga caggaataca cgtggcatct tatggaaaag atttgggtaa tattaatttg
    10621 ttaaaattac ttgaaatggt gcatactatt aagggattgg aaagaatccg cttaagttct
    10681 attgagcctt ctattgttaa tgaggaattt gtaaatacga ttcaaggtct tcacaaaata
    10741 tgcccgcatt ttcatttatc attacagagt ggttgtgatg aaactttaaa aagaatgaat
    10801 agaaaatata caacaaaaga ttataaagag gccgttgaat tattaagaga caagattgag
    10861 gatattgctt taacgacaga tattattgtt ggtttcccag gggaaaatga tgaagagttt
    10921 aatcaatcct atcattttgt taaggaaatt gcattttctc aaattcatgt ttttaaattt
    10981 tcccccagac aaggcacgcc ggctgctcgg atgaaggatc aaatagctcc tgaggtcaaa
    11041 gaagaaagaa gccggaaaat gattgaactt ggtcagttct tacaaaatca gtttttagtc
    11101 cagcaaatcg gaaagaccat ggatgtatta tttgaaaata ctttggacga ccatgaagaa
    11161 atttttgaag gtcatacgcc gaattatctt aaagtggaag tgcattcaaa agaagatata
    11221 aaaaatcaaa tcctccccgt aacttttcaa ggaattaaaa atcatagctt aataggtaat
    11281 attaaaaatt gataacaaat tccaatatcg tttgcacaaa gacttttttt gtattatgat
    11341 agaaatatat attagtcttt gttatttaat atggagatct ttaggggagt gagaagaatg
    11401 agtgagttca atgaaaccgt tcaatttaat gtagataaag ataaaggaaa tgaaataagg
    11461 gatattttat tctatgttta tgaagcacta aaagaaaaag gttataatcc agttaatcaa
    11521 attgtcggct atatattatc tggtgatccg acttatataa ccagttataa caatgccaga
    11581 agcatcatta gaaaattgga aagagatgag cttttagaag aattaataaa aagttatctt
    11641 gaaaaaaatt aaggagttta tacttcatgt ttaaacggat tttccttacg ataactgttt
    11701 tgatattttt ttttatggca gataaaagct atggagaaag tacaaaaagc atgttattaa
    11761 tcgtaacaga aagtagttgg gatgcgtttt tggcttctcc ttttatttct tcttttattc
    11821 cggagagtag tgttggttca atgaatatcc gcacaaatca ccaaaacaaa atcattgaat
    11881 attatggaac catcaatgca ggaagaagat ggagttatta tgaggtcaat gatatggggg
    11941 ctgtagataa aggattgggg gattatctga accaatcggg tttatctaca gctttttttt
    12001 cggataagga tgaattaata aaaatcattg aaaatcaata tggaatgacc agttatagaa
    12061 cgaacgattt cgaaaaatta ttttcagacg ataacaaaaa aattttagaa acagcagatg
    12121 ttattttaat tgatatggat ttatattgca ctaaagaaaa ccaggatttt ttattagatt
    12181 ttattaaaaa aagacatgtc cgtctctatg ttttttgccc ttatcatgtg gaaaataaat
    12241 taactccttt tttattttat gatagcagaa aaccttatcc aggtttaatt acatcagcaa
    12301 ccaccagaag gcaagggatt attaccaatt tagatatagc tccttctatt ctgtcttatt
    12361 atggtattga ttgtagttct atggttagta aaggcataga aatacaatac attccggatg
    12421 cttataagaa gttaaatcat ctattagaaa aaattgaatt tttaaattat tatcgttcaa
    12481 atataatcaa gatatataat ttttttctaa tttctttgtt aattatttat ttttttcttg
    12541 aaaaaaggaa tcattatatg tttaatataa tttatgatgc ttttattatc tttgctttat
    12601 ggctgcctgt ttta
//
LOCUS       DAOSUD010000062        12329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_2981_length_12329_cov_28.893841, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000062 DAOSUD010000000
VERSION     DAOSUD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2981_length_12329_cov_28.893841"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..636
                     /locus_tag="PLG49_08195"
     CDS             73..636
                     /locus_tag="PLG49_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ17336.1"
                     /translation="MSEIERHLKDWEFLIALENKHVAYILHSLDNFKCKIMQMYSCCA
                     SFQVAETLALQLKDARGKRVLNYIKRKKGKFAFHSEETLNILRQYWNKFKTQITMGTV
                     QKIFEYAHKASGIKKDASSHRLNQGYKTYLLEYGTKYRHIQELLSHGNNITKIYFYGS
                     TNSLAQAKSPFDSLNPNRKEAINEEIR"
     gene            620..745
                     /locus_tag="PLG49_08200"
     CDS             620..745
                     /locus_tag="PLG49_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17337.1"
                     /translation="MRKFVKNWWIWQYLDRRNSLYVNERGMASCLKIKMFDYERI"
     gene            732..3938
                     /locus_tag="PLG49_08205"
     CDS             732..3938
                     /locus_tag="PLG49_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOQ17338.1"
                     /translation="MREFKKSQNKVANHNSLPKLADSLVSKTKNLLREAKTEEDLRIG
                     FEKLLEPIRTELNLTSTPKYEKSVYSGRSDAVHGQVIIEYEPPRSFSSKKNIDHANEQ
                     LVNYLSDEAEKTKLTQWVGVGFDGEQIFFVQYQGENSKAVDKTKFVMRGPYDFTPESA
                     RTFLIHLRALSRLPLTAENLAQKFGPQSEIAPKAVSAFANALEYWGVKAHIRTFFNEW
                     KRLFGIVYGEQFNEGHQEKETEILSKLYKVGAETDFQELLFSIHTYFAFLMKLIAVEL
                     LTLQETSFSSSLTSQLAHVSDDELKRQLEDIENGGIYARKGITNFLEGDFFRWYLDTF
                     ESPELKDAIREIARALSEFEPATSTLDPVSTRDLLKKLYQYLVPQEVRHRLGEYYTPD
                     WLAELLLNEVGYDGNTLKRFLDPACGSGTFLVLAIQRAIRHGREKERIPDLEIVKRIV
                     ANIWGFDLNPLAVIAARTNYLFALGDLVNEILDRGGKIEIPIYLCDSVLTPTRTTGNL
                     FGEFLEVSTSVAKFQVPAEWVRDGGFLLANAAPLVEEMVKNQYSVNEALERFKKEGLV
                     FSHNEQVVKDFYNQILELEKQNKNGIWARFLKNSFAPMVAGKFDFVVGNPPWILWEFL
                     SKEYRNATSNLWQDYKLLPKGFSRPDFSMLFTYASADYYLKDGAKLGFLITQEVFKSK
                     GAGEGFRRFQLGNKKYLKVLKAHDLVSIQPFEGAANKTAAIILKKGERTEYPVPYFIW
                     SKKKGVGKIATDKLLDEVLLLLQKKKYNAKPIGSTLGSWQTQPAGFEELSKIEGKNFY
                     KAQYGVETKPYGVFWVKINQFLSDRSVIVSNIPEKGKVKIQKIEERIESDLIYPALRG
                     ADIKRWGKTNEIFILLTHDKNGKPIEENILRTTFPRTYGYLTKFKRDLLNINAKSTKK
                     IRQQKAFYAMFGVNEDTFSNYKVVWGYMGNDILATVVSEIKTGSNYKKLIPLYTTCFF
                     PVYNESEAHYLCAIINSKPVRDFIKSFSSAGRGFGTPSAMEHVGIPKFDPKNPLHQKL
                     AKISKKCHQLKLEGKEKEIEKLEKENDELVCELFNIKG"
     gene            4096..4824
                     /gene="cas6"
                     /locus_tag="PLG49_08210"
     CDS             4096..4824
                     /gene="cas6"
                     /locus_tag="PLG49_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOQ17339.1"
                     /translation="MGIVFKSENKVILPLSYNEIVQGFIYGNLSPTVAEHLHEEGFKD
                     GKRSFRLFTFSRLLGNFTIQNDSFQIKSPFKLIISSPHRDTLQNLAENLLKSPEAELG
                     NQKVTIESISAHFTPPLEGEAEIEMLSPVTVYSTLTRADGGKKTYYYNPKEEEFSVLI
                     RENIIKKYKALYEKDPGGTEFHIEPLKVLKKDEKIITYKGFVIKGWMGTYKISGTPEL
                     LKIAYEAGIGAKNSQGFGCFEVIK"
     gene            4839..6470
                     /gene="cas3"
                     /locus_tag="PLG49_08215"
     CDS             4839..6470
                     /gene="cas3"
                     /locus_tag="PLG49_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOQ17340.1"
                     /translation="MRCDEFYREYLNIQNPYEFQVKTWQLIQEPKFPLLIKAPGGSGK
                     TEAVVAPFLAQFVKNEFSLAPRLIYTLPIRALANTIAKRIEKYAKRISQKISVNVQHR
                     DVHNSPFFIGDIIVTTLDQFLYGFARTSMQVGHHIDIPAGSIASSFVVFDEAHMYRDS
                     FTFSIMRALMEILYESKVPFVVMTATMPDSLEESLFENIGKQDVNAIDNIPSFSKIDI
                     KFSEEPLYAGEVNICDQILSKIKHKKTLIVLNQVKRAQKVYDEINKRLALNNSGIVLL
                     HSRFTKVDREQHEDIATSLLPRKDDGEIKTPKGIGIVVSTQVLEAGIDFSAELLLTEL
                     APADSLVQRAGRCARYQGEIGEMIIFPTEKENKGYLPYEKEPIERTREWLRSNPNFNI
                     KDFNQVRSFVNETLNYRANDYEARDTLIDLYECVLYADQKPTNIQLRNGKPITLVVCD
                     LSNGAKDKIEDKIKDAIRRTNIRENSIDVDIGVGLKLLNDDGVLKYALSFDNEKREWI
                     PSKVNDISPFGYYLIDRNFYCPLKGVLADAESFII"
     gene            6448..7167
                     /locus_tag="PLG49_08220"
     CDS             6448..7167
                     /locus_tag="PLG49_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOQ17341.1"
                     /translation="MQKVSLYSDPNENYLCHISRCKNVFEIIFPVFNHTLSRIIPSQM
                     NEDSFVKMILFHDLGKLTHKWQTNIQANKRLPSHSTIGAAYLWKILPNGIKEPISFAV
                     AIHHTDKGLLGDNIEKPDVQAILEGIVDNSGNIIWHEETKNLSEEYFPYKVRELNISD
                     LKEMARGLRNWAKGCGILEQHQRRLKASLAHHILKLCDISAATERKEYQKKDDQDYYG
                     GWLMVENIRNYVDAIRERIKL"
     gene            7164..8582
                     /gene="csa5"
                     /locus_tag="PLG49_08225"
     CDS             7164..8582
                     /gene="csa5"
                     /locus_tag="PLG49_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-A CRISPR-associated protein Csa5"
                     /protein_id="HOQ17342.1"
                     /translation="MSITLYTPATGFPDLEVKIAYGLARVGVEAFGMEKVTIRDNGGF
                     YSILINVDETEFPKLDSTFNMLCKRLLSSEFIPSNTPGIGSRSAKSINVKENESFSLE
                     IYKSMEFLSENKSNENTCRHKHSSVGNIIGLTASTSFHNRRDGLDIQSQSMNPKDKTS
                     PKVPRRPTNPKNICKTCALLAILGMWYASFIFNIKGKEIIALPVPEGEVSDTRLQQIF
                     SLHHQIRKSWFNLEIPQTLIPLIFLANVPSSADILKQFDLFIAVLSRQQGYHVDGLYL
                     MPIEKYLSFLSDNPFNIAAIDLMVEKEAFSALQELNNIIYYDKQDSIMMFARLYVQET
                     STNNFTNLLYPKTAKYLLKEVAMISQDIIENRALESLARTLRYFIIEKKYGYADDIRN
                     ARKESRDFEETIAKMLREGRLRLEQKEKIHLPTDEEIKEVFRLANQDFEATKTTLVIL
                     AFSFPSASQKPEEVITEEVSNA"
     gene            8575..9582
                     /locus_tag="PLG49_08230"
     CDS             8575..9582
                     /locus_tag="PLG49_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HOQ17343.1"
                     /translation="MLKFISFAVKTQLNVHDLNNEAVAGNVADIRIMEFIDEEGKRQE
                     APAVSGRMLKHWHYEGMRNLGKQQKLPFCDSCRIGEPVRPAKLKSDGILSQKDAIEGE
                     QISVVKNCVICDIHGYLATIKGEDKKEQENEEEQEDKREQKGKGKGVSERRSSRVMFS
                     WLMPVLGSEFPSKQVIHSRVKSDREIDDKNLASQMLFNKSYASGIYAFVSALDVDRIG
                     LVELNLGTQNPYVIDDNSRKERIRVAIEAYRYLISGQMGASLSHAIPHMNPKEIVVVY
                     SEIGPLPFPVSPMYSDYISKTAGLMPKDAKFLYWGPETPEGFSKKDTIDGIFAELLSK
                     V"
     gene            9585..10319
                     /gene="cas5"
                     /locus_tag="PLG49_08235"
     CDS             9585..10319
                     /gene="cas5"
                     /locus_tag="PLG49_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOQ17344.1"
                     /translation="MKALVFNIRVNSLYSIRIPFTWQSALTYPILPPSAVIGMLANAL
                     QRYKNNKHPVEYLELIEDNLLWAGSRLLKPCIIKSYTMSAITKWEDAIGGKFTNALGR
                     QFAYTELLEISAIFRNDILIEDIVNALQTSPVTCGDSESLVSLDGEVMVKNVEETSNI
                     EDITTEYPVPFSMDTHIKEGKGQMYLMHERCKKTSESFPLILYLVPIEEKNGIFSKSE
                     IRIEKTADMVYKIQDVGYIVMRAQNR"
     gene            10316..10858
                     /gene="cas4"
                     /locus_tag="PLG49_08240"
     CDS             10316..10858
                     /gene="cas4"
                     /locus_tag="PLG49_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOQ17345.1"
                     /translation="MKDDFDQTSGQAEKNRPTKPSGTLIWYYYICPREVWFMSRQLNP
                     SQENPFIEIGKLISEESYERERKEVRVDNIVIDVLKDSGGKVLVGEVKKSSRYEKSAM
                     MQLCYYLLRLKYLGIEAKGLLLFPKEKKRIEVNLSPEIENELENAINEIEMIKQMSTP
                     PKLKKTRFCAKCGYIEVCWS"
     gene            10855..11847
                     /gene="cas1b"
                     /locus_tag="PLG49_08245"
     CDS             10855..11847
                     /gene="cas1b"
                     /locus_tag="PLG49_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOQ17346.1"
                     /translation="MKRTLYIFSNGELKRKGNTIYFESNEGKKYIPVENIQEIFAFGE
                     VTMNKKLFEFLCESEIILHFFNYYGYYVGSYYPREHLNSGFMILKQAEHYLDESKRVS
                     LAKKFVIGAVENINRVLAYYKNRGKDLNEVIEKIDSLSQHINRCTTTEELMAIEGNIR
                     EKYYKAFDKILDNEHFVFQARSKRPPLNRINALISFGNSLLYTVCLSEIYNTHLDPRI
                     GFLHTTNFRRFTLNLDVSEVFKPIIVDRVIFNLVNKSMIKPQHFESKLQGIVLNEKGR
                     EIFIREFDERLRTSFHHRGLKRNVTYKTLIRLELYKIEKHLMGDEEYKPFISSW"
     gene            11855..12118
                     /gene="cas2"
                     /locus_tag="PLG49_08250"
     CDS             11855..12118
                     /gene="cas2"
                     /locus_tag="PLG49_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOQ17347.1"
                     /translation="MYVILVYDVQVERVQKVLKTCRKYLYWVQNSVFEGEITQAKLEK
                     LKHEINKIIEDDTDSVIIYTFLSERYSNREILGVAKGGMDILL"
BASE COUNT         4385 a         2156 c         2396 g         3392 t
ORIGIN      
        1 gccctcaata tttttaatcc agcagttagt gctctataat tttactattg cgtgatgctt
       61 ggaaaagagt tcgtgagtga gattgaaagg caccttaagg attgggagtt tctaatagct
      121 ttagaaaaca aacatgttgc gtatatcctc cattctcttg ataattttaa gtgtaaaata
      181 atgcaaatgt atagttgctg tgccagtttt caggttgcag aaacattagc cctgcagtta
      241 aaggacgcac gcggtaaacg agtcctgaac tatatcaaac gcaaaaaagg caaattcgca
      301 ttccattcag aggagacttt aaatatcttg aggcaatatt ggaataaatt caaaactcaa
      361 attacaatgg gaacagttca aaaaatcttt gagtatgctc ataaagcttc gggaataaag
      421 aaagatgctt cgtctcatag gttgaatcaa ggttacaaaa catatttatt ggaatatgga
      481 actaaatata gacacattca agaattattg agtcatggaa ataatataac aaaaatttat
      541 ttttatggta gcacgaatag tcttgcccaa gcgaaaagtc catttgatag tctaaaccca
      601 aataggaaag aggcaataaa tgaggaaatt cgttaagaac tggtggattt ggcagtattt
      661 agatagaaga aatagtttgt atgtgaatga acgaggcatg gcatcatgcc taaaaataaa
      721 gatgtttgat tatgagagaa tttaaaaaaa gccaaaataa agtagctaac cacaattcgc
      781 ttccaaaatt agcagatagt cttgtttcta aaactaaaaa tctgcttcgc gaagctaaaa
      841 ccgaagaaga tttaaggatt ggctttgaaa aacttttaga gccgataaga accgaattaa
      901 atcttacatc tactcctaaa tacgagaagt ccgtttatag cggacgctca gatgcggtgc
      961 acggacaagt aataattgaa tatgaaccac caaggtcgtt ttcttcaaag aaaaacatag
     1021 accacgccaa cgagcagtta gtaaattatt tgtctgatga agcagaaaaa acaaaactaa
     1081 cccagtgggt cggcgtgggt tttgacggtg aacaaatttt cttcgttcaa tatcaaggtg
     1141 aaaatagtaa agcagtagat aaaaccaaat ttgttatgcg tggtccctat gattttacgc
     1201 cggaaagcgc cagaactttt ttaattcatc tacgggcatt atctcggctt ccacttaccg
     1261 ccgaaaattt ggctcaaaaa tttggtcccc aaagtgaaat cgcaccaaaa gcggtttctg
     1321 cgtttgccaa tgcgttggag tattggggtg tcaaagcaca tatccgcaca ttttttaatg
     1381 aatggaaaag actatttggt attgtttacg gtgaacaatt taatgaaggg catcaagaaa
     1441 aagaaactga aatactttcg aaactttaca aagtaggagc agagacagat tttcaagaac
     1501 ttttgttttc catccatact tactttgctt ttttaatgaa acttattgct gttgaacttt
     1561 taaccttaca ggaaacatcc tttagttcgt ctcttacatc tcaacttgct catgtttccg
     1621 atgacgaact taaaagacaa cttgaagaca tagaaaacgg cggaatttat gcccgaaaag
     1681 gaataactaa tttcttagaa ggtgattttt tccgttggta tctcgatact tttgaatcac
     1741 cagaattaaa agatgctatc agggaaattg cccgtgctct ctccgaattt gaaccagcaa
     1801 ccagcacttt agaccctgtt tcaactcgcg atcttctcaa aaaactttac cagtatttag
     1861 tcccacaaga agtccggcat cgtctcggtg aatattatac acccgattgg ctggcagaat
     1921 tattgctaaa tgaagttggc tatgacggta acacgctcaa aagatttttg gacccggctt
     1981 gcggttccgg tacttttctt gtcttggcaa ttcagcgagc aatcagacac ggtagggaaa
     2041 aagaaagaat acctgatctt gaaatcgtaa aaagaattgt cgctaatata tggggatttg
     2101 atttaaatcc attggcagtt atcgccgccc gcaccaatta tttgtttgct ttaggagatt
     2161 tagtaaatga aattttagac agagggggaa agatagaaat tccaatttat ctttgtgatt
     2221 ctgttttgac tcccacaaga accacaggaa atctttttgg tgaatttttg gaagtttcta
     2281 catctgtcgc aaaattccaa gtccctgccg aatgggtgcg ggacggcgga tttttgctgg
     2341 caaatgccgc gccgcttgtc gaagaaatgg taaaaaatca atactcagta aatgaggcat
     2401 tagaaagatt caaaaaagag ggtttagttt tttctcataa cgaacaagtc gtaaaagatt
     2461 tttataacca aattttagaa ctggaaaagc agaataaaaa cggcatctgg gcaaggtttt
     2521 tgaaaaacag ttttgcgcca atggttgctg gtaaatttga ttttgtcgtc ggcaatccgc
     2581 cgtggatatt gtgggaattt ctttctaaag aatatagaaa tgctacttca aatctatggc
     2641 aggattataa gttactccca aagggattca gccggcctga tttttcaatg cttttcactt
     2701 atgcttctgc agattattac ttaaaagacg gggcaaaatt aggatttttg ataactcagg
     2761 aagtttttaa atcaaagggt gccggtgaag gatttagaag atttcagttg ggaaataaaa
     2821 aatatcttaa agtattaaaa gcacatgatt tggtttctat ccagccgttc gaaggagcag
     2881 ctaataaaac cgccgctata attcttaaga aaggcgaaag aacggagtat ccagttcctt
     2941 attttatttg gtcaaagaag aagggcgtgg gtaaaattgc cacagataaa ctattagacg
     3001 aagttttgct gcttttacag aagaaaaaat acaatgctaa acctattggg tcaactcttg
     3061 gttcctggca aactcaacct gctggatttg aggaattgtc taaaattgaa ggaaaaaatt
     3121 tttataaagc ccaatacgga gttgaaacaa aaccttacgg ggtattttgg gtaaaaataa
     3181 atcaattctt atctgacaga agtgtcattg tctccaacat accagaaaag ggcaaagtga
     3241 aaatacaaaa aatagaagag agaattgaat ccgatttaat ttatccggct ctgcgaggag
     3301 ccgatattaa aagatgggga aaaacaaatg agatctttat attattaacc catgataaaa
     3361 acggaaaacc aatagaagaa aacattctta gaactacttt tccaaggaca tacggatact
     3421 taacaaaatt taaaagagat ttattaaata tcaatgcaaa atctacgaaa aaaatacgac
     3481 aacaaaaagc attttatgca atgtttggag taaacgaaga tactttctct aactacaaag
     3541 tagtttgggg atatatgggt aatgatattt tagctacagt agtatctgaa atcaaaacag
     3601 gttcgaatta taaaaagtta atccctttat atactacctg ttttttccct gtttataacg
     3661 aatccgaagc tcattatctc tgcgccatta tcaactcaaa acctgttcgt gatttcatta
     3721 aatcattttc ttccgctggg cgcggttttg gcacaccttc cgctatggag catgtcggca
     3781 ttccaaaatt cgaccccaaa aatcccctcc accaaaaact cgctaaaatt tccaaaaaat
     3841 gccaccaatt aaaacttgaa ggaaaagaaa aagaaattga aaaattagaa aaagaaaatg
     3901 acgagttagt ttgcgaactc tttaacataa agggatgata tataagtcaa acctctttag
     3961 gggtgtgttg tacctgctat ttttaagacc gtataaaata ttatttcatg attaaaaatc
     4021 acgaccacaa ccccttcttg acaaaagacc aagtatacta caaaatttaa caaaatctaa
     4081 tttagataaa tgagattggg gattgtattt aaatcagaaa ataaggtgat acttcctcta
     4141 tcatataatg aaattgttca aggctttatt tatgggaatt tatcccccac cgtagcagaa
     4201 caccttcacg aagaagggtt taaagatgga aaaagaagtt tcagactttt cacgttctca
     4261 aggctattag gaaattttac aattcaaaac gattcttttc aaataaagag cccctttaag
     4321 ttaatcattt cgtcacccca cagagacact cttcagaacc tcgctgagaa ccttctaaaa
     4381 tcaccggaag cagaacttgg aaatcagaag gtaacaattg aatcgattag cgcccacttt
     4441 accccacccc tcgagggaga agcggaaatt gaaatgctct cacctgtgac cgtttacagt
     4501 actttaacac gagccgatgg aggcaaaaaa acatattatt acaaccccaa agaagaagaa
     4561 ttttcagtct taataagaga aaacattata aaaaaatata aagcacttta tgagaaagac
     4621 ccaggaggaa cggaatttca tattgaacca cttaaagtct taaagaaaga tgaaaaaatt
     4681 attacttaca agggctttgt tataaaaggt tggatgggaa cgtataaaat atccgggacc
     4741 ccagagcttt taaaaattgc atacgaggcg ggtattggag caaaaaactc tcaggggttc
     4801 ggatgctttg aggtaataaa gtaaaggggg ttaaaaagat gagatgtgat gagttttata
     4861 gggagtacct caatatccaa aacccgtatg aatttcaggt aaaaacatgg caattaatac
     4921 aggaaccgaa atttccactt cttatcaaag cacctggtgg ctcaggtaaa accgaggcag
     4981 tagttgcacc ctttttggct cagttcgtga aaaatgagtt ttctctcgct ccacggctga
     5041 tctacacatt gccaataaga gccctggcta acacaatagc aaaaaggatt gaaaagtatg
     5101 caaaaaggat ttctcagaaa atttccgtga atgttcagca tagagatgta cacaacagtc
     5161 cgtttttcat cggggacatt atagtaacga cccttgacca attcctctac ggttttgcga
     5221 gaacaagtat gcaagttgga catcatatag acattcctgc aggttctatt gcatcctcct
     5281 tcgtcgtgtt tgacgaagcc catatgtata gggacagttt cacattttca attatgagag
     5341 cgttaatgga gatactttat gaaagtaaag ttcctttcgt tgtaatgacc gcaacaatgc
     5401 ctgatagcct tgaagaaagc ctttttgaaa acattggcaa gcaagatgta aatgcaattg
     5461 acaacatccc aagtttctca aaaatcgaca taaagttttc agaagaacct ctctatgcag
     5521 gagaagtaaa catttgtgat cagatcctta gcaaaatcaa acataaaaag actctaatcg
     5581 tgctgaatca agtgaaaagg gcacagaaag tttacgatga aattaacaaa aggcttgccc
     5641 taaacaatag cgggattgtt ttgctgcact cacgctttac aaaagttgac agagaacaac
     5701 acgaggatat agccacttca ttgctacccc gaaaagatga tggagaaata aagactccaa
     5761 aaggtatagg aatcgtagtc tcaacacagg ttcttgaggc aggaattgat ttttctgcag
     5821 aattattact tacagaactt gcaccggcag attcactggt tcaacgggca ggtagatgtg
     5881 cacgatacca aggcgaaata ggggagatga ttattttccc tactgaaaag gaaaataaag
     5941 ggtatttacc atatgaaaaa gaacctatag aaaggactcg tgaatggctc cgcagtaatc
     6001 cgaattttaa cataaaggac tttaatcaag tacgttcctt tgttaacgag actcttaact
     6061 atagggcaaa tgactatgag gcacgtgata ccttaattga cctctatgag tgcgttttat
     6121 atgcagatca aaaaccaact aacattcagt tgagaaatgg taagccaata actcttgtag
     6181 tatgcgatct ttcaaatgga gcaaaagata aaatagaaga caagattaaa gatgcaataa
     6241 ggagaactaa tatacgcgaa aactcaatag atgtggatat tggtgttggg ttgaaattgt
     6301 tgaacgacga cggtgttcta aagtatgcgt taagctttga taatgaaaaa agagaatgga
     6361 tcccctcaaa agtaaacgat atctcgccct ttggttatta tttaattgat cgaaattttt
     6421 attgtccatt aaagggggta ttagccgatg cagaaagttt cattatatag cgacccaaat
     6481 gagaattatc tgtgccatat ctcaagatgt aagaatgtct tcgagataat ctttcctgta
     6541 ttcaatcaca ctctgagtag aattattcct tcacaaatga atgaggattc tttcgttaaa
     6601 atgatactat ttcacgacct gggaaagctc acgcacaaat ggcaaaccaa tattcaggca
     6661 aataaacgtc tgccatccca ctccaccatc ggggcagctt acttatggaa aattttacca
     6721 aatggcatca aggagccgat aagttttgca gttgccattc atcataccga taagggactg
     6781 cttggggaca acatcgaaaa acccgatgtt caagcgatcc ttgaaggcat cgtagataat
     6841 tccggcaaca ttatatggca tgaggaaacg aaaaatttat ctgaagagta tttcccatac
     6901 aaagtaaggg aactaaacat ctctgatttg aaggaaatgg caaggggctt gagaaattgg
     6961 gctaaaggct gtggtatact tgagcagcat caaaggagat taaaggcttc ccttgcccac
     7021 catatattaa aactctgcga catttctgct gccaccgaga gaaaagaata tcaaaaaaaa
     7081 gatgaccagg attactatgg tggatggctt atggtggaga atataagaaa ttatgttgat
     7141 gcaatcagag agagaataaa attatgagca ttacccttta tacccctgca actgggttcc
     7201 cggatttaga agttaaaatt gcctatggat tggcaagggt cggagttgaa gcttttggca
     7261 tggagaaggt tacaatacga gataatggcg gcttctactc cattttaatt aacgtagacg
     7321 agactgaatt tcccaaactt gatagcacat ttaatatgct ctgcaaaaga cttctctcat
     7381 ctgaattcat tccatccaat acgccaggca taggtagtag gagtgccaaa agtataaatg
     7441 ttaaagaaaa cgagtctttc tcactcgaga tttataaatc aatggaattt ctctcagaaa
     7501 acaagtcaaa cgaaaatact tgcagacaca aacacagttc ggtcggcaat atcataggtt
     7561 taactgcatc aacttcattc cacaatagga gagatggctt agacatccaa tctcaatcaa
     7621 tgaatccaaa agacaaaaca tctcctaaag tacccagaag acctacaaat ccaaaaaaca
     7681 tttgtaaaac atgcgcttta cttgcaattc ttggcatgtg gtacgcctcc tttatattta
     7741 acatcaaagg gaaagaaata attgcactac cagtaccaga aggtgaggtt agtgacacaa
     7801 gattacagca aattttctct ctgcatcatc aaataagaaa gagctggttc aatttggaaa
     7861 ttccacaaac tcttatcccc ctgatctttc tggccaatgt cccatcatca gcagatattc
     7921 taaagcaatt tgacttgttc atcgcggtac taagtcgtca acaaggctat catgttgacg
     7981 gactttattt aatgcctata gaaaagtact tgagcttctt aagcgacaat ccatttaata
     8041 ttgcagcaat agacttaatg gtagaaaaag aagctttttc agcacttcaa gaactcaaca
     8101 atattattta ttatgacaaa caggattcaa ttatgatgtt tgcccgcctt tatgttcaag
     8161 aaacatcaac aaataatttt acaaatcttt tatatccaaa aacggcaaaa taccttttaa
     8221 aggaggtagc tatgataagt caggatatca tagaaaatcg tgcattggag agtcttgcga
     8281 ggactctaag gtactttatc atagaaaaga agtatggcta tgcagatgat ataagaaatg
     8341 cgagaaaaga gtcaagggat tttgaggaaa caattgcaaa gatgttgaga gaaggaaggt
     8401 taagattaga gcaaaaagag aagatacacc ttccaaccga tgaagaaata aaggaagtct
     8461 tcaggcttgc aaatcaagat tttgaagcta ctaaaactac tcttgtgatc ctcgcttttt
     8521 cattcccctc agcatcacaa aagcctgaag aagttataac agaggaggtg tcaaatgctt
     8581 aaatttatat cttttgcagt aaaaacccag ttaaacgtac atgacctcaa taatgaggct
     8641 gtagctggca atgtagcaga tattaggatt atggaattca tagacgaaga aggtaaaaga
     8701 caggaagcac cggcggtatc aggaaggatg ctaaaacatt ggcattatga aggaatgaga
     8761 aacctgggca agcagcaaaa attacctttt tgcgactcat gtaggattgg agaacctgta
     8821 agacctgcaa aactaaaatc agatgggatc ctttctcaaa aagatgctat tgagggggag
     8881 caaatatctg ttgtcaaaaa ttgtgttatc tgtgatattc atggatatct tgctacaatt
     8941 aaaggagaag ataagaaaga acaagaaaat gaggaagaac aagaagataa gagagaacaa
     9001 aaaggtaagg gaaaaggcgt ttctgaaagg agaagttcaa gagttatgtt ttcatggtta
     9061 atgcctgttt tgggtagtga atttccctca aaacaagtta tccattcacg agtcaaaagt
     9121 gatagagaaa ttgatgataa aaacctggct tctcagatgc ttttcaacaa atcctatgcc
     9181 tctggcattt acgcatttgt atcggctctt gatgtggata gaattgggct cgtagaacta
     9241 aatcttggga cccaaaatcc ttacgttatt gacgataatt cacgaaaaga aagaataagg
     9301 gttgccattg aagcctaccg ttatttaata tctggacaaa tgggggcttc tttatctcat
     9361 gcaatccccc atatgaaccc aaaagaaatc gtagtagttt attctgaaat tggaccttta
     9421 ccgttccctg tatcaccgat gtattcagat tatatttcca aaacagctgg tttaatgcct
     9481 aaggatgcaa aattcctcta ttggggtcca gaaaccccag aaggatttag taaaaaggat
     9541 accattgatg ggatatttgc tgaactctta agcaaggtct aaaaatgaag gcattagtct
     9601 ttaacataag agtgaactct ctgtactcaa taagaattcc cttcacctgg caatccgccc
     9661 ttacttaccc tatattgcca ccatctgctg ttatagggat gctcgcgaat gcattgcagc
     9721 gatacaaaaa caacaaacac cccgtagaat acctcgaact tatcgaagat aacttactgt
     9781 gggcaggttc acgactatta aaaccttgta taatcaaaag ctataccatg tctgctatta
     9841 caaaatggga ggatgcaata ggcggtaaat tcaccaatgc ccttgggaga caatttgcat
     9901 atactgaatt attagagatt tctgcaatat ttcgtaatga tattttaata gaagatatag
     9961 ttaatgccct acaaacatct cccgttacct gtggagatag tgaatcttta gtatccttag
    10021 acggtgaagt tatggttaaa aacgtagaag agacttcaaa tatcgaggac atcacaactg
    10081 aatatccagt acccttttca atggataccc acattaaaga gggtaagggg cagatgtatt
    10141 taatgcatga aagatgcaag aaaacatcgg aatctttccc attaatacta tatcttgtac
    10201 caattgaaga aaaaaacggg attttttcca aatcagaaat tcgcatagaa aaaactgccg
    10261 atatggtgta taaaattcag gacgttggtt atattgtgat gagggcacaa aacaggtgaa
    10321 agacgatttt gatcaaacct caggacaagc agaaaaaaac agaccaacaa agccttctgg
    10381 tactcttata tggtattact acatatgtcc tcgagaagtg tggtttatga gcaggcaact
    10441 taatccttct caagaaaacc ctttcatcga aattggaaaa ctcattagtg aagaaagcta
    10501 cgagcgggaa agaaaagaag tcagggttga caatatagtc atcgatgtct taaaagacag
    10561 tggaggaaaa gttttagttg gagaggtgaa aaagtcatca agatatgaaa agagtgcaat
    10621 gatgcaactt tgctattatc tactgaggtt aaaatacctt ggaatcgaag ctaaaggctt
    10681 gttactgttt cctaaagaaa agaaacgcat agaagtcaat ttatctccag aaatcgaaaa
    10741 cgagttagaa aatgcaataa atgaaatcga aatgattaaa caaatgtcga cacccccaaa
    10801 actgaagaaa accaggttct gcgctaaatg cggttatatt gaggtttgtt ggtcatgaaa
    10861 agaacactat atatattctc aaatggggaa ttaaaaagaa aaggaaacac aatttatttc
    10921 gaatctaatg aagggaagaa atacattcca gtagagaata ttcaggaaat atttgctttc
    10981 ggcgaagtta caatgaataa gaagctcttc gagttccttt gcgaatctga gattatattg
    11041 cattttttta actattatgg atactatgtt gggtcttatt acccacggga acatctaaat
    11101 tcgggtttta tgatccttaa acaggctgaa cactatctcg atgagtctaa aagggtttca
    11161 cttgccaaaa aattcgtcat tggtgcggta gaaaatataa atagagtgct cgcatactat
    11221 aaaaacagag gaaaagatct caatgaagta attgaaaaaa tcgactcgtt aagccaacac
    11281 attaacagat gcacaaccac ggaagaacta atggcaattg aaggtaacat aagggagaaa
    11341 tactacaaag cttttgataa gatacttgac aacgaacatt ttgttttcca agccagaagt
    11401 aaaagaccgc ccctcaatag aattaacgca cttataagtt ttgggaattc actactctac
    11461 acagtatgcc taagcgaaat ttacaacact catttagacc caagaattgg ttttctacat
    11521 acaacaaact tcagaagatt taccctgaac cttgatgtat ctgaagtttt taaaccgata
    11581 atagtagata gggtaatctt caatttagtc aacaaatcta tgataaaacc tcaacatttt
    11641 gaaagtaaac ttcagggcat tgtgcttaat gaaaagggaa gagaaatttt cattagagaa
    11701 ttcgacgaac gattaaggac aagtttccac caccgaggat taaaaagaaa cgtaacttat
    11761 aaaactctta tccgcctcga gctgtacaag attgaaaaac accttatggg agacgaagag
    11821 tacaaaccct ttataagctc atggtgaaag aaaaatgtat gtgattctag tttacgacgt
    11881 acaagtagaa agggtacaaa aggtcttaaa aacatgcaga aagtaccttt actgggtgca
    11941 aaattcagtt ttcgaaggtg aaatcaccca agctaaactt gaaaaactta agcatgaaat
    12001 caacaagatt attgaagatg acactgactc tgttataata tacacattcc tcagcgagag
    12061 gtacagcaat agagaaatat taggcgtagc taaaggaggc atggacatac tgttatgaga
    12121 tttacattct caatatcgtc tacctcaatt aatgtaaaac gatgcatgtt acgacgattg
    12181 caaaaacata gacagcccag gcaaatcgca atataataaa caagtaaagc agctctttgt
    12241 aaatttcgta tgcttgggat ataatataaa aatgggtttt gagcctacct ataaggaatt
    12301 gaaacttatg tgttctacga ctatctctg
//
LOCUS       DAOSUD010000063        12065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3046_length_12065_cov_19.029808, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000063 DAOSUD010000000
VERSION     DAOSUD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3046_length_12065_cov_19.029808"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLG49_08255"
     CDS             <1..309
                     /locus_tag="PLG49_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ17348.1"
                     /translation="RRLFYVGITRAKKYLAISTVKKRYEEEVKPSRFIEEMTKKPSTE
                     EFQKGAIIVHKKYGRGKISNIDGTTAAIAFEKGIFSRKIDLKYCIDKELIYIEKDETN
                     "
     gene            355..795
                     /locus_tag="PLG49_08260"
     CDS             355..795
                     /locus_tag="PLG49_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17349.1"
                     /translation="MFFGRRSKEKQRKLAAFLAFLIVIAMILSIILPFVTYAAETEDI
                     IIKGEIGFNNKYKVGGTTPITIEITNNGEDFKGEVQVKILKEEYNNVTNYIVYAKDIE
                     LPRGSTKKFDMVVTTSNMQRYFNIELTDGKKYCQENDLCYCFSA"
     gene            731..2842
                     /locus_tag="PLG49_08265"
     CDS             731..2842
                     /locus_tag="PLG49_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17350.1"
                     /translation="MNLLMGKSIAKKTIYATAFPPESRFVGVLSEDPQSLKYLKNVGF
                     GEVIGNKLIELDENSFPTDINILNDFETVFINNFDTSKLSKEQINIMNQWVQSGGILI
                     LGTGPNSDKVLKGLGSEIASVVNKGITHTTDFTEMEQLGEKTLDSGQPLQIALLDIKN
                     GEDILFSGNLPITTLIHEETGSVIIHHFDLGINPFAQWDGNRYMLTELYIKHIPVFAQ
                     TSNTSDSYDYDPYLNPYTLRLFPRQQQTGLVIGVLIIISVYIILVGPILYWILKVKDK
                     REYGWFIIPILAFAFSGGIYLLSYKTIFNAPVGSSIGIVRLNTGETTSSLSISAGFFT
                     PDTGESKITFAENIQPRVSYVDSSWDFRNRVINNGIKTEVISAKIRIGNANELIFYND
                     QSWAMNTVTASHTVNFAGPINASLTFKDNNLIGTIENHLGFDLETCILVVGDHYYYLD
                     TLKNDDAMYIEKQISSRAKYRYEALDEIFGSVYDINAMNKKWGRELSKEEIHRLIQRR
                     EIFVNSNDRYHITTNSFNAQNIYGNNESTIVLYGYSEESLFGDVKINDKEIRNYNQNL
                     FIIPLEIDYSKADQIEIPYGIIKPYITGNLGFSVEEYDQSIYIHSSGSLDLTYSIDPS
                     IKLDEFQIQFMKSSSVDGSYIFNNESGIWEELSILPYEENVQKYRNSEGQIQIRFDVL
                     VDDQKDNRMEIPKLRLKGDKQ"
     gene            2842..3778
                     /locus_tag="PLG49_08270"
                     /pseudo
     CDS             2842..3778
                     /locus_tag="PLG49_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3771..4625
                     /locus_tag="PLG49_08275"
     CDS             3771..4625
                     /locus_tag="PLG49_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17351.1"
                     /translation="MINPVLRRELKTKMRTWKAPTLLVIYLAILAVFGGLILSINELE
                     SYTGGFDPGNALTIYFLLAGCQLGLIIILVPALTSGTISGERERQTLDLLLITKLSPF
                     SIIIGKLMASISQIILLILASMPVFSIIFLFGGVSASNILLLFFFFIATAVMVGSIGI
                     FCSTFFRKTTTATVVSYLLILILSIGTLVGLALLNQYAYIVRQQGLTYGESLLIAGAN
                     PFVGFLSVIQNQAGYNIVSDILGVRNNISKIPFQPWQVNMIFDAIITCIMIFFSVIRI
                     RPVMRRRK"
     gene            4668..5024
                     /locus_tag="PLG49_08280"
     CDS             4668..5024
                     /locus_tag="PLG49_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17352.1"
                     /translation="MTELYKALKPLRKKIILEKWMQYIVIGCIAGMTLDLGIIVLSKW
                     VLIPNLFIILASVILLSWLCASVTVLFKLPGIEDIAKKGDALGYKERFITAIEISQKG
                     DNFFNSHVQISTGGCS"
     gene            4993..6243
                     /locus_tag="PLG49_08285"
     CDS             4993..6243
                     /locus_tag="PLG49_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17353.1"
                     /translation="MYKLALEDAVNAAKNADFDKRYSISLPKKKLWIIGILMVGTIVA
                     GFTPSPVKSKLELQLEIKEKAKSEIVKIEKLQKELENNPKISSKELKRLNKELDTLKA
                     DLKKVNTKGEIVRASQRTQQQLKKISKESVQGDLRKLGEKLSSHELTRALGDQLQQGN
                     IAEIRKNLDDLQNRLKKMDKDQIQQLASILKDAANEIENNSELQQILNDYSQAVKSGD
                     LEGLADEYEKLGQILEYLSMENQDLREAIENISKAMASNNPNNKENEENQQQSQGSGT
                     EEEEGQKQGSETGKGQGEGQGQGQGTGQGEGQGQGSGTGQGQGQNKSGGQGRGQGHKP
                     NEKIYSRQAQDKQDYDAQVDGIKNQSGDMHKVDQKTIGNRGVSVPYEEVYQQYRQDAI
                     KFLEDDTIPYGIKNLVEEYFSSLE"
     gene            6301..10755
                     /locus_tag="PLG49_08290"
     CDS             6301..10755
                     /locus_tag="PLG49_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ17354.1"
                     /translation="MNFYNPWGFLGLIAIPIIILMYLLKQKYEEVKVPSVFLWEQALS
                     YSQAYQPWQKLRKNLLLFIQLLAAALLVIALANPYLTGKWEKSGNYIFILDSSMSMQA
                     EDEKPNRFENAKNQMEHIINSLEPNAEISLIVMEKEPYILVNKSTEKNTVIKKLREVE
                     VSNTEVNSEETASLVQALYEQTKGKIYLFSDENMFVPDLDIEFVKVGKNSDNCGIVLL
                     SHSKEEGQLAVLVRVKNFGKTPRKVSVSLYGDEEFIDVKEAELSADQESNVFFAGVPK
                     NIEILKAEIDSDDVLKTDNIMYDVVQEDVKQKVLLITEQNIFLEQMMSILPQVELYKG
                     DFENQEVPKGYYLYIFDGKMPSVLPEDGHILIFNPEANNPLVQVEGEVEVDHILPSGS
                     RLFEFIKEMDFDIAKAKKMVLPEWGEEILGSDQTPLIFFGELQEQKVLICGFDLHDTD
                     LPLKKEFPIFIYNIMQWFIPSEANNLKNITAGDTIEFDILPEAREVNLVDPKGNIEKL
                     APPFPVQPYKNIDELGIYVLEQKGENRDVFYSFAVNPPTELESDLDRTNQDNVQVGNK
                     ASEEEKIARNRNLKNIFILLVLLLLVFEWWLYIRREKKVFLTSIRCIIAILLVFSLMG
                     FEIRKTIDATTTIFAVDLSDSSKASIKEAENFIQQAIAFKSDHDEVGIVSFGGNAAVE
                     VSPKSEPVFSGFDTIINAHFTDISQGLKLSSSLIPEESKKRIVLISDGQENLGDALSQ
                     AKILAGQNIKVDVVPVQKSVSDEVQITDLTLPQYLNLHQEFSVQIRVDSLVDTQGILK
                     IYNGKNLVETQKVNIRKGQNRFAFSDKAEEGGGRIYRVELEADKDSMNENNVAYGYTY
                     VEDIPHLLLIEKDHSGEEIRKLLEGTKVEINAIEPYLSPTQMDELSQYDGVILANISG
                     DDLDEKFLGNLEYYVSHLGGGVVVTGGENAYALGNYFDTPLETILPVEMELKDKNNIP
                     DMGLMIVTDRSGSMEEAPYGISKLELAKEAAIRAAEVLHSFDQIGVIAFDDMPQEIVK
                     FQKIDNNLESIKNDIASISPGGGTSIIPALRKAYETLYSADTKVKHIILLTDGQAERS
                     GYSELLGQMKASGITLSTVAVGRSSDTVLLEELAQNGGGRYYYTDEFSDLPKIFMKET
                     MMAGKTYINTETFYPKVTGISPILKDIEELAPLHGYISTTPKNRAEVILKSEKDDPVL
                     AVWRYGLGKTAVWTSDVHNQWSADWLSSDSGVRIIRNMISWVLRKPMSDTIKVEGKVI
                     GDQIELSAEVQNSDLTEEIEVNVVKPDLTQETLVLKPTAPGKYTGRFDGNDLGAYMLN
                     FQINRNGETESIHTGINISYSSEYDIRKITAETTLLSKIAQLTGGKILTDAKEVFKPI
                     EDSIYGKKDITEILLILSLLLYLLDIAYRRVGFIHSIIDNGILLIAKKMRNRKKEYNK
                     EKTQKQELKRPKTKKPKVKEKQKSVTQEQMKENTSMVLLNQKRKRSKK"
     gene            10860..>12065
                     /locus_tag="PLG49_08295"
     CDS             10860..>12065
                     /locus_tag="PLG49_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOQ17355.1"
                     /translation="MMEREYIDQQNRIIEAPSITIKNVLIALGYFIDNEKESFLNPSD
                     KCKKMIRDLVLHIVQKHSHRGTKIHHIQDRIILLKYSIMEVITKWKVLEEHELKYVGA
                     FFDHVINQFTDIAVNDFQTAFETLARPILNLNNYDELCGEIVSKTCSLLPQDYCSLVT
                     FYNDTHSVRISDYTGELFSELMHPIDWELIDKVRKEKITYMKTVRKKDRLQDWAQISG
                     PIEINNKVVGVLSLHYRSAHHFDYDEQKMLEILCRQAAVSIHTLKLYKKLSYAYQNSK
                     FVNEELRLTKSRLEESNKNLLELDRLKSMFIANISHELKTPLNTIIGFSELLMSSLED
                     KIPEQNLKDLNNIYQSGKYLLRLINDILDLSKIEAGKVEFENEEVDLYEIVLNGAMTL
                     RSLIGDKNIE"
BASE COUNT         4281 a         1738 c         2465 g         3581 t
ORIGIN      
        1 agaaggctgt tttatgtggg gatcacaaga gcaaagaaat acttggcaat atcaaccgtc
       61 aagaaaagat acgaagaaga agtaaaacct tcaagattta ttgaagaaat gacgaaaaaa
      121 ccttcgacag aagaatttca aaaaggcgca atcattgtcc ataaaaaata cggcagagga
      181 aaaatcagca atattgatgg aactactgcc gctatcgcat ttgaaaaagg aattttttca
      241 agaaaaattg atttaaaata ttgtatagac aaggaattaa tttatattga aaaagatgaa
      301 acaaattagc ctatttttac gttcatgtat ttatgaaaca tgaggaggga tactatgttt
      361 tttggaagac gctcaaaaga aaagcaaaga aaacttgccg cttttttagc atttttgatt
      421 gtaatagcca tgattttaag tattatactt ccctttgtta cctatgctgc agaaactgaa
      481 gatattataa tcaaaggtga aataggattt aataacaagt ataaagtggg aggaacaact
      541 ccaattacca tagaaataac caataatgga gaagatttta agggagaagt acaagttaaa
      601 atcttaaagg aagaatataa caatgttacg aactatattg tgtatgcaaa agacattgag
      661 cttcccagag gttctacgaa gaaatttgat atggttgtta ctacttccaa tatgcagcgt
      721 tatttcaata ttgaacttac tgatgggaaa aagtattgcc aagaaaacga tttatgctac
      781 tgcttttccg cctgaaagca gatttgtagg tgtattatca gaagatcctc aaagtctgaa
      841 atacttaaaa aatgttggct ttggtgaagt cattggcaat aaattaatag agttagatga
      901 gaatagtttt ccaactgata tcaatatatt aaatgatttt gaaacagtgt tcattaataa
      961 ttttgatact tcaaagctca gcaaggagca gattaatatt atgaatcagt gggtacaaag
     1021 cggtggaatc cttattcttg gaactggacc gaattcggat aaggttttaa aaggtttggg
     1081 ttcagagatt gcttctgtag ttaataaagg aattactcat accacagatt ttactgaaat
     1141 ggagcaatta ggagaaaaaa ctcttgattc tggtcagcct cttcagattg ctcttttgga
     1201 catcaaaaat ggagaggaca ttttattttc aggcaatttg cctattacta ctttaattca
     1261 cgaagaaaca ggcagtgtta ttattcatca ttttgattta ggtattaatc catttgcaca
     1321 gtgggatggg aataggtata tgcttactga attgtatata aagcatatac ctgtttttgc
     1381 acagacgagc aatacatcag acagctatga ttatgatcct tatttaaacc cctatacatt
     1441 acggttgttt cccaggcagc agcaaacagg tttagtaata ggggttttaa ttataataag
     1501 tgtatacatt attcttgtag gccccattct ttactggatt ttaaaagtaa aagacaaaag
     1561 agaatatggt tggttcatta ttcctattct tgctttcgct ttttcaggag gaatttactt
     1621 attaagttat aaaacgatct tcaatgctcc agttggaagc agtattggaa tagtacgttt
     1681 aaatacgggt gaaacaacat cttccttaag tatcagtgca gggtttttta ctcctgatac
     1741 gggagaaagt aagattactt ttgctgaaaa tattcagccc agagtctcat atgtagacag
     1801 ttcatgggat tttagaaaca gagtaatcaa taatggcata aagacagaag taatttctgc
     1861 aaaaattaga ataggaaatg ccaacgaact tattttttat aatgatcaaa gctgggcgat
     1921 gaatacagtt actgcaagtc atactgtaaa ttttgcaggc cccatcaatg catcccttac
     1981 ttttaaagat aataatttaa ttggcactat tgaaaatcac ttagggtttg accttgaaac
     2041 atgtattctg gttgtaggag atcattatta ctatctggat acactaaaaa atgatgatgc
     2101 aatgtatatt gaaaaacaga tttcttctag agcaaagtat cgatatgaag ctttggacga
     2161 gatattcgga tcggtatatg acataaatgc catgaataaa aaatggggac gggaattatc
     2221 taaagaagag atccacagat taatacaaag aagagaaatc tttgtaaatt ccaatgatag
     2281 ataccatata accacaaata gttttaacgc tcaaaatatt tatggtaata atgaatctac
     2341 tattgtactg tacggttata gtgaagaatc tctttttggg gatgtaaaaa ttaatgacaa
     2401 agaaatcagg aattataatc aaaacttatt tattatacct cttgaaattg attattcaaa
     2461 agcagatcag atagaaattc cctatggcat tataaaacct tatattacag gaaatcttgg
     2521 atttagtgtg gaggagtatg atcaatctat atacattcat agcagcggta gtttggatct
     2581 tacttattcg attgatccta gtataaagct tgatgaattt caaatacagt ttatgaaaag
     2641 ctctagtgtt gacggatcat atatttttaa taatgaaagt ggaatatggg aggaattaag
     2701 catactgcct tatgaagaaa atgttcaaaa gtatagaaac tctgaaggac agatacagat
     2761 tcgttttgat gttttagtgg atgaccagaa agataataga atggaaattc cgaaattacg
     2821 cctgaaagga gataaacaat aatgttagaa atacagaatt tagttaaaaa aatatggtaa
     2881 gttcactgct gttgatggtt tatctctgac ggttaatgat ggagaaattt ttggatttgt
     2941 tggaccaaac ggagcaggaa aaacaactac tatgaaaatt atggcaggac ttttaagctc
     3001 tacttctggt aatgtattaa ttaatggagt agatgttaca gcgcatccaa gaaaattgag
     3061 agagaaaata ggttatatgc ccgacttttt tggagtctat gacaacctga aggtagatga
     3121 atatatggat ttttatgcag gaacctatca tattccctat agtgaaagag aaactattat
     3181 taataattta ttggagttag ttgatttatc acataaaaaa gatgcttatg tagatacact
     3241 atccagagga atgaagcaaa gattatgtct tgccagaagt cttatacacg atccggatat
     3301 tctcattttg gatgaacctg catcgggatt agatccgagg gccagagtag aaatgaagga
     3361 agtactaaag cagttgaaaa ctttaggaaa aactattatt atcagttctc atattctgcc
     3421 ggaattagct gagttatgta ctgtaatcgg tattattgat aaaggaaaga ttgcggcata
     3481 tggtactgtg gctcagatta tgcagcagct gactcagaaa agaattatta aaatcaaagt
     3541 aatgggcaat atggatcaac tgattacaat tttaaaagaa cagcccaatg ttcatgagat
     3601 tattgaaaaa gtagatgaag ttgaaattga atatgatgga gacgatcata gtcttgctta
     3661 tcttctaaaa aatatggttc aacaagatat ccccattatt tccttttcag aaaaagaagg
     3721 aaatttggaa gaaatcttta tgcaaattac agaaggaggc gaaagtgatc atgattaacc
     3781 ctgtacttcg tcgtgaatta aaaacaaaga tgcgaacatg gaaagcaccg actttactgg
     3841 ttatatattt agcaatattg gcagtgttcg gaggtcttat cctgtcaatc aacgaacttg
     3901 aatcttatac cggtggattt gatcctggaa atgcgttgac catatatttt cttcttgcag
     3961 gctgccaatt gggattaata attatattag tcccggcatt aacctcggga accataagtg
     4021 gtgaaagaga aagacaaact ctagatcttc ttcttattac taaactatcg cctttttcta
     4081 ttatcatcgg gaaattaatg gcgtctatca gtcagattat tttgctgata ctagcttcaa
     4141 tgcctgtttt tagtattata ttcttatttg gaggcgtttc agcatcgaat attttactat
     4201 tgttcttctt tttcattgct actgcagtta tggttggaag tataggcata ttttgctcaa
     4261 cattttttag aaaaactact actgctacag tggtctcata tttacttatt cttattcttt
     4321 ccattggtac ccttgtggga ttggctttgc ttaaccagta cgcttatatt gtcaggcaac
     4381 aaggattgac ctatggagaa tctttgttaa ttgccggagc taatccgttt gtaggatttt
     4441 tatccgtaat tcaaaatcag gcaggctata atattgtaag cgatatactt ggcgttcgta
     4501 acaatatttc taaaattcca tttcaacctt ggcaggtcaa tatgatattt gatgctatca
     4561 ttacctgcat aatgatattt tttagcgtga tcaggatccg tcctgtgatg aggcgaagaa
     4621 agtaacagaa ttttaaagat gcatattagt ataaggaagt ggatattttg acagagcttt
     4681 ataaagcatt aaaaccttta cgaaagaaaa ttattttgga aaaatggatg caatatatcg
     4741 ttattggctg cattgctggt atgactttgg atctcggtat tatagtattg tccaagtggg
     4801 tactgattcc taatctgttt ataattcttg catctgtcat tcttctttca tggctttgtg
     4861 ccagcgtaac agtattgttt aaattgcctg ggatagagga catagctaaa aaaggtgatg
     4921 ctttaggata taaagagcgt ttcattaccg cgattgaaat atcacaaaaa ggggataact
     4981 tttttaactc ccatgtacaa attagcactg gaggatgcag ttaatgcagc taaaaatgca
     5041 gattttgata aacgttattc tatttcactt cctaagaaaa aattatggat cataggtata
     5101 ttgatggttg gaacgattgt tgcagggttt actcctagtc ctgtaaagtc aaaacttgaa
     5161 ttgcagcttg aaataaaaga aaaagcgaaa tcagaaattg taaaaataga aaagttgcaa
     5221 aaagaattgg aaaataaccc taaaatatct tcaaaagaat taaaaaggct taataaagag
     5281 ctggatacgt taaaagcgga tttaaagaaa gtgaatacta agggagaaat tgttcgtgca
     5341 tcacaaagaa ctcagcagca acttaagaaa atttctaaag agtcggttca aggcgactta
     5401 agaaaattag gagaaaaact atcaagccat gaattaacaa gggctctggg tgaccagcta
     5461 cagcagggga atatagctga gatcagaaaa aatctggatg atctccaaaa ccggctgaag
     5521 aaaatggata aagatcaaat ccaacaactg gcaagcatct taaaagacgc tgctaatgaa
     5581 atagaaaata attctgaatt acaacagatt ttaaatgatt attcacaggc agtgaaaagt
     5641 ggtgatcttg aaggtttggc agatgagtat gagaaattag gccaaatatt agagtatttg
     5701 tctatggaaa accaggattt aagagaagcc attgaaaata tcagcaaagc aatggcatct
     5761 aataacccaa ataataagga gaatgaggaa aaccaacagc aaagtcaggg cagcggaaca
     5821 gaggaagaag aagggcaaaa gcaaggcagt gaaactggta aaggccaagg agaaggacag
     5881 ggccaaggac agggaacagg tcaaggcgaa ggtcaaggac aaggaagcgg aactggtcaa
     5941 ggtcagggac agaacaaaag tggtggtcaa gggcgagggc aagggcataa gcccaatgag
     6001 aaaatttatt caaggcaagc tcaagataaa caggattacg atgcacaggt tgacggaata
     6061 aaaaaccagt cgggagatat gcataaagtt gatcaaaaaa ctatcgggaa cagaggagta
     6121 tctgttcctt atgaagaggt ttaccaacag tatagacaag atgcgattaa atttttagaa
     6181 gacgatacga ttccatatgg aattaaaaat ttagtggagg aatatttctc ttccctggaa
     6241 taatacattt ggtgcctgtc tgtctcaaca gacaggcatc atttaaaaag aggtgtttcc
     6301 atgaattttt ataatccatg gggtttttta gggcttattg ctatacccat cattattttg
     6361 atgtatcttt taaagcaaaa gtatgaagaa gtaaaagttc caagtgtatt tttatgggag
     6421 caggcattaa gttattctca ggcttatcaa ccctggcaaa agcttaggaa aaatcttttg
     6481 ctttttatac agcttcttgc agcagctctg cttgtaattg ctttggcaaa cccctatttg
     6541 accgggaaat gggaaaagtc agggaattac atattcattc ttgacagttc catgagtatg
     6601 caggcagaag atgaaaaacc taatcggttt gaaaacgcaa agaatcaaat ggaacacatt
     6661 attaactctt tggagcccaa tgcagaaatt tctcttatag tgatggaaaa agaaccttat
     6721 attctggtga acaagtccac tgaaaaaaat acagtcataa aaaagcttag agaggtagag
     6781 gtatccaata cagaggtcaa cagtgaagaa acagcttcgt tggtgcaggc cctttatgag
     6841 cagacaaaag gaaaaatcta tttgttttct gatgagaata tgtttgttcc tgatttagat
     6901 atagaatttg tcaaagtagg taagaactct gataattgcg ggattgtatt attatctcat
     6961 tcaaaggaag aagggcaatt agctgtactg gtaagagtaa aaaattttgg aaagacacct
     7021 agaaaagtat ctgtgtccct ttatggagat gaagaattta ttgatgtaaa ggaagcagaa
     7081 ctttcagcgg atcaggaatc aaatgttttt tttgcaggag ttccaaagaa tatagaaatc
     7141 ttaaaagctg aaatcgattc agatgatgtt ttaaaaacag acaacattat gtacgatgtg
     7201 gtacaagaag atgtaaagca aaaagtttta ctcattacag aacagaacat ttttttagaa
     7261 cagatgatgt ccatacttcc ccaagtggaa ttatataagg gcgattttga aaaccaagaa
     7321 gtacccaagg gatattattt gtatattttt gatggcaaaa tgccttctgt tctgcctgag
     7381 gacggacata tactgatctt taatcctgaa gcgaacaatc ctttggttca agtagaagga
     7441 gaggtagaag ttgatcatat tttgccgtcg ggcagcagac tatttgaatt tataaaagaa
     7501 atggattttg atattgctaa agccaagaaa atggttcttc ctgaatgggg agaagaaatt
     7561 cttggttctg atcaaacacc tttaattttc tttggagaat tacaggagca aaaagttctt
     7621 atctgcggtt ttgacttgca tgatacagat ttaccgctaa aaaaagaatt tcctattttt
     7681 atatataaca tcatgcagtg gttcattcct tctgaggcca ataatctaaa aaacatcact
     7741 gcaggagata ccattgagtt tgatatattg ccggaagctc gggaggtcaa tttagtagat
     7801 cctaaaggga atattgagaa attagcgcct ccttttccgg tacagcctta taaaaatatt
     7861 gatgaattgg gaatatatgt acttgaacag aaaggtgaaa acagagatgt tttttactct
     7921 tttgcagtca atcctccaac agaattggaa tctgatttgg acaggactaa tcaagataat
     7981 gtccaagttg gtaataaagc ttctgaagaa gaaaagattg caagaaatcg taatttaaaa
     8041 aacatattta tactcttagt gttgcttttg cttgtctttg aatggtggtt atacattcga
     8101 agagaaaaga aagtatttct tacaagtatt cgatgtatca ttgcgatatt gcttgttttc
     8161 tccttgatgg gatttgaaat caggaagacg atcgatgcaa ccaccaccat atttgccgta
     8221 gatctatccg atagttctaa agcatctata aaagaggcgg agaattttat tcagcaagcc
     8281 attgctttca aaagtgatca cgatgaagtt ggaattgtga gttttggagg caatgcagca
     8341 gtggaagtga gcccaaagtc agaaccagtt ttttcaggat ttgatacgat tattaatgcg
     8401 cattttacag atatttctca gggattaaag ctatcttctt ctcttattcc ggaagaatcc
     8461 aaaaagcgga ttgtactcat ttcagatggg caagaaaatc tgggagatgc attaagtcaa
     8521 gcaaaaatct tggcagggca gaatataaag gtggatgtag ttcctgtcca gaagtctgtt
     8581 tctgatgaag tacagatcac ggatttaaca cttccccagt atttaaatct gcatcaggaa
     8641 tttagtgttc aaataagggt ggacagctta gtcgatactc agggaatttt gaaaatatat
     8701 aacggaaaaa atttagtaga gactcaaaag gttaatattc gaaaaggaca aaatagattt
     8761 gctttctccg acaaagctga agaaggggga ggcagaattt atcgtgttga actggaagcg
     8821 gacaaagaca gcatgaatga aaataatgta gcttatggct atacttatgt ggaagatatt
     8881 ccccatttgc ttttaattga gaaggaccat agcggggaag agattagaaa attgttggaa
     8941 gggacaaagg ttgaaatcaa tgcaattgaa ccttatcttt cccctaccca aatggatgaa
     9001 ttgtcccagt atgatggggt tattttagcc aatatctcag gggatgactt ggacgaaaaa
     9061 tttctgggca atttagagta ttatgtgagt catttgggag gaggagtagt cgtaaccggt
     9121 ggagaaaatg cttacgctct ggggaactat tttgatactc ccttggagac aatactgccg
     9181 gtagaaatgg agttaaaaga taaaaacaat attccggata tgggacttat gattgtcact
     9241 gacaggtccg gaagtatgga agaagcccct tatggtatct caaaactgga actggcaaaa
     9301 gaagcggcta ttcgtgcagc agaagttctg cactcctttg atcaaatagg agttattgca
     9361 tttgatgata tgcctcaaga aattgtaaag tttcaaaaaa tagataataa tcttgaaagt
     9421 attaagaatg atattgcaag tatttcgcct ggaggaggaa ccagtattat tcctgccctt
     9481 agaaaagcct atgaaacgct ttacagcgca gatactaaag ttaaacatat tattcttctt
     9541 acagacggtc aggcagaaag atcaggatac agcgagctcc ttggacaaat gaaagcatca
     9601 ggcatcactt tatccacagt agctgtggga agatcttctg atacagtttt attggaggaa
     9661 ttggctcaaa atggaggagg aagatattat tataccgatg agttttctga tttgcctaaa
     9721 atttttatga aagaaacgat gatggcagga aaaacttata tcaataccga aactttttat
     9781 ccaaaagtaa caggaatttc tcctatattg aaggatatag aggaattggc gccacttcat
     9841 ggatatattt ccacaacgcc taaaaaccgt gcagaagtga ttttaaaaag cgaaaaggat
     9901 gatcctgttt tagctgtctg gagatacgga ttaggtaaaa cggctgtatg gacttcggat
     9961 gttcacaatc agtggagtgc tgattggctg tcctcagatt ctggggttag aattatcaga
    10021 aatatgattt catgggtttt aaggaagcca atgagtgata cgattaaagt agagggaaaa
    10081 gttataggtg atcagatcga actaagtgca gaagtccaaa attcagatct aacagaagaa
    10141 attgaagtga atgttgttaa accggattta actcaagaga ctctggtgct taaacctaca
    10201 gctcctggca aatacactgg gagatttgat ggaaatgatt tgggagctta tatgttgaat
    10261 ttccaaatta acaggaatgg agaaacagaa tccattcata cgggaatcaa tatatcttat
    10321 tcttcagaat acgatattcg caaaatcact gcagaaacta ctcttttaag taaaattgcc
    10381 cagttaacag ggggaaagat tttaacagat gcaaaagaag tctttaagcc tatagaagac
    10441 tcgatttatg ggaaaaaaga tattacagag atcctgctta ttctaagtct tttactatac
    10501 cttttggata tcgcctatag aagagtaggt tttattcaca gtataataga caatggaata
    10561 ctcttgattg ctaaaaaaat gagaaatcga aaaaaagaat acaataagga gaaaactcag
    10621 aaacaggaac taaaaaggcc taaaacgaaa aagccaaagg ttaaagaaaa acaaaagtct
    10681 gttacccaag aacaaatgaa agagaataca agtatggtat tattaaatca aaagagaaaa
    10741 agatcaaaga agtagatgga tcaaaaactg tatttgaatt cagaggtgag gagattgggc
    10801 cattttgtaa acagatgtga aagatatatg gatgacattt tatttggctg ggaaaaaata
    10861 tgatggaacg agaatatatc gatcagcaaa atagaatcat tgaagcacct tcaattacaa
    10921 taaaaaatgt tttgattgct ttgggatatt ttattgataa tgagaaagaa tcttttctga
    10981 atccatcaga taaatgcaag aaaatgatta gggatttggt tcttcatatt gtgcaaaagc
    11041 atagtcacag aggaaccaaa attcatcata ttcaagatag gattattctt ttaaaatatt
    11101 cgattatgga agtcattacg aaatggaaag ttttagaaga acatgagtta aagtatgtgg
    11161 gtgctttttt tgatcatgtc atcaatcagt ttacagacat tgctgtgaat gatttccaaa
    11221 cggcttttga aacattagca aggcctatct taaatctcaa caattatgac gaattatgtg
    11281 gagaaatcgt atctaaaacc tgctcactgc tgccacagga ttactgttct ttggttactt
    11341 tttataatga tacccacagt gttcggattt cggattatac aggagagtta ttcagtgaac
    11401 tgatgcaccc tattgattgg gagttgattg ataaagtcag aaaagaaaag attacgtata
    11461 tgaaaacagt cagaaaaaag gatagattac aggattgggc acagatttcc gggcctatag
    11521 aaatcaataa caaagttgta ggagtcctta gccttcatta cagatctgct catcattttg
    11581 attatgatga gcaaaaaatg ttggaaatct tatgcagaca ggcggctgtt tccattcata
    11641 ctcttaaatt atataaaaag ctttcttatg cttatcaaaa ttcaaaattc gtgaatgaag
    11701 aacttagatt aaccaaaagt cgattggaag aaagtaataa aaatctatta gagttagata
    11761 gattaaaatc catgtttatt gcaaacatat cccatgaatt aaaaacccct ctaaatacga
    11821 ttattggttt ttctgaactt cttatgtcca gtctggaaga taaaattccg gaacagaatt
    11881 tgaaagatct taataatatt taccaaagcg gtaaatacct tttaagactc atcaatgaca
    11941 ttttggattt atcaaaaatt gaagccggaa aagttgagtt tgaaaatgaa gaagtggatc
    12001 tatacgaaat cgtattaaat ggggcgatga cattaagatc actgatcggg gataaaaaca
    12061 ttgaa
//
LOCUS       DAOSUD010000064        12011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3055_length_12011_cov_26.134326, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000064 DAOSUD010000000
VERSION     DAOSUD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_12011_cov_26.134326"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2269)
                     /locus_tag="PLG49_08300"
     CDS             complement(<1..2269)
                     /locus_tag="PLG49_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17356.1"
                     /translation="MKGINQLKIFFLILVMMMTSMPQMTYASDDAQLESQEIVVNNDF
                     GNDSKDYFAEELSTEGPAAKDETVAERVYELPQKSLIVDQPVIFRDDFFEANFTILEE
                     SEDTFIGEIILENTSLVSFKDWELEFNLNQEIIHIENAEIVRCRYNQYTISGVDSTAY
                     FYAGERIRIIFEAKLDGNFEFPDYFSLDGEIDFDHYPEDETEYKIWKEQVLARKNNNV
                     SSRSFRLQAASEAKKGSIGNDYIRFTYVNGPHGVMTTGGDPDNPYDDYRDLLYGSGLS
                     SLATIRIDGENYDFENLDSVTTFENKMIGTKQIGDIIVYQQLSIVYNPYTQREDTVEF
                     LYTVKNTGDTPHTVGVRLLLDTVFDGNDLVPFKIPGIGERAIETDLWGDDIPEYWQIF
                     NSVTNPKIIAQGILKVDKEYMPDRVRFTNWTSARLNMWDYYVPEGQSNGDSAVALYWN
                     ERTLGKDASFSCKTFFGLGKLEQDLTPPLSLAVMGTTELGVLKNEAGEEEYSPNPMTI
                     SAFIENIGTGTATNVIATLNLPEGMEIVEGSQSMYLGDLPAGAKKYDLLWKVKVQPTW
                     SEKVAKYSVTITADNTDSKTVEKEIVIPKFTKVGGQGITFTHIKDTDYKITLKTKNRI
                     DKAVIQFKNPNGWNWALFDDIKDDDRFIMIPVENDGNPELFTYEKIIRLTTTGRREFR
                     ILFLNSKNEIIEKSDSQYINAGKLVSFDTDRTDYKLIIKKHSEVVNKIKVCIGKQFDN
                     IGVSNTGINTEILYFI"
     gene            complement(2522..4438)
                     /gene="cooS"
                     /locus_tag="PLG49_08305"
     CDS             complement(2522..4438)
                     /gene="cooS"
                     /locus_tag="PLG49_08305"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOQ17357.1"
                     /translation="MDETILEKTEGRVSYHDSVEEMLKRIREDGMSNVFDRWTLQEKI
                     RCKFCLQGLSCQLCSQGPCRISEKGGQDRGVCGIGADAMAMRNLLLQNIMGAGTYSHH
                     AYEAFRTLRATGEGKTPFKITDVDKLKWMCEKLGIDTNQDINQMAIDLANFLEHQMKI
                     GKDEPNIMVEVFASKKRKELWKKTHLYPAGVNHEEEICVASCLTNVDGDYASLAKKAL
                     RLGLATIYNAQIGLEMVQDILFGTPKPHEVTVDLGIMDPDYVNVVFNGHQPWAGVAVL
                     EKARDPKIQEKARAAGAKGLRVVGSIETGQELLQRFEVDDVFVGLMGNWLAIEPLLAT
                     GTVDVMAMEENCSPPAIDMYAEKYQVTLVSVSTIIGIPGLQHAIPYHPDKVGEMADQL
                     IDLAIENFKKRKGKIVPRVPKITQKAIAGFSTEAVLEVLGNKLDPLVDVIAAGKIKGV
                     VALANCSTLRNGPQDWNTVNLTKELIKKDILVVSGGCGNHALELAGLCNLEALNIAGE
                     GLRQVCSALKIPPVLSFGTCTDTGRISMLVSALADHLDVDVSDLPIAVTAPEWMEQKA
                     TIDGVFAVAYGAYTHLSPTPFVTGAPALVKLLTEDVESITGGKIALGDDPVEVANNIE
                     THILSKRKAMGLKI"
     gene            4623..6443
                     /locus_tag="PLG49_08310"
     CDS             4623..6443
                     /locus_tag="PLG49_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ17358.1"
                     /translation="MSFIFISSSSNDKAIADAIYENLEKKGIKCWNSDKNIHANQNYN
                     AAILQAIHNCRAMVFIYSSNAESSQDVLREVKEASENNKIIIPFIIENKIPSQNMEAY
                     LQSNYKIDATVTSIYESIYKLENLLKNLLAAQRNNVPHAVIPQAPVDYSNNFNSPNPN
                     RPVPAKKSKAPLIIGISAAAVVCLILLAGGIFGMSLLLAPTDESYIEEITTTSSNGAY
                     DIGFKVEQDDDLYGSRFTEEEIIEFAKEAIRELDRTNSDSEFYQNYGYQGPEYKDLYN
                     VQEDVLFAMMGLEVVRFDDVKVMGKNKKDFGIEYLLSMDFTVYANYDRETETGETEHI
                     EGEASLYNNVVIIETNDGELKYLYMESIKQEELENRYEKINSFYEEMKEKEVIEAEDT
                     VQAYVPSDANINNNSLKSVKEIVEENDHKVVAVFVDTPEGEVQGSGFFIEDGIIVTNF
                     HVIEGGRSAYVRLTDERFIEVEGIVCANPALDLAILKLTEQIGVEPVRLGGISDVQKG
                     EMAVAIGSPLGLFNTVSTGIISNTWNQDGVHLIQISIPITHGNSGGALFDDSGKVIGV
                     TTSGIGDANLNFAVSTEHLLEVCELIKEIGYDNIEVVPFK"
     gene            6517..9495
                     /locus_tag="PLG49_08315"
     CDS             6517..9495
                     /locus_tag="PLG49_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOQ17359.1"
                     /translation="MPKYDAFISYRHTEPDKTIAEKLHKMLETYKVPSKIAKKTGKKK
                     INRVFRDKEELPTSSNLAASIEDALINSEYLIVICSPRTPESKWVLKEIETFSKLHGH
                     DKILALLIEGEPIDAFPEQLRFVTKEMIDNEGNVIEEKEEVEPLAADIRGKDTKELIK
                     NLKKELLRLLAPILGCNFDDLRQRHRERFIKRVITISICFSLFFLAFGSFSTWQAIQI
                     KQQVNKTLEGQSLYLANLSRQVLNEGDRRTAILLALEALPKNLEKPERPYVEEAEYAL
                     TEGLNIYNTSNTFMSDIVLNHRKSVSNIQISPDHSKVFTVCNDGKIYLWDTSNGNLLY
                     TFPHNSITLSKEQSSFSANGEIIVSITSDGLAAWSTTTGVKLWEIEATIPRFVLNPDG
                     KSLALIQQEPTKNHLKLIDINNGNVFIHMTFERKNFELVNAMAFNQDGSILALGTDQG
                     LIQLVDIQNQKNLYRLNSANENIKKVFFSDDGKKIAAFSGNFDLETADIYSTGTGNIE
                     IWDTESTQNILNHKLNTSTIAEASFSPVNSNIVAYTENELVKVVDISSGEILFTFTHG
                     SKVTNFKIFNDGDFFVTTSLDGNIMFYSLDIGISLPSWNIYHNDSISVMDIEDNILAL
                     SASTSNKAYLLKSLDNPYVTTMSGHSSTIEKGKFSPSGQKVLTVAESEVFIWDIEKRS
                     LFNKLNGIEGTILNADFTPDESKISILTKEGYIKIFDMSSGSELSSLNTGSFTNYYYS
                     PSNSLVALQIDDMVKIFDCYNCKELLTINNNSYLNKSFFSNQDKFVLIGKWDGEFSLY
                     DIKTAKILHTFQNKENLKINVIAFNKDDSLLALNQGDRIYIYDTKNFEMVGYIENLLF
                     APSVITFSHDNRELFVGLDDKSIQIYDLETGQYKTELTGHNQILKECRYSPDGNLLIT
                     IGEFGECILWNPVNYKKLAYINKVLDINHSFTNFLSFNYQDVILMPRYDLESLVRIAK
                     SELGNRKLSKEEKIKYYIIE"
     gene            complement(9587..10078)
                     /locus_tag="PLG49_08320"
     CDS             complement(9587..10078)
                     /locus_tag="PLG49_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HOQ17360.1"
                     /translation="MKYIINQKIFSIGDKFVIKDEYGSERYFVEGKVFSLGDKLKIYD
                     SNGQERIYIEQKLFKLLPEYYLYFDGNFAAKVKQAFSLLRPKFQIESPFGSYSVEGDF
                     FGYDFRIMKNSRLVAHVSKKFFSFRDSYGVDIVNDENDLLILAVVIVIDQVIHDEDHH
                     RNH"
     gene            complement(10090..11589)
                     /locus_tag="PLG49_08325"
     CDS             complement(10090..11589)
                     /locus_tag="PLG49_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ17361.1"
                     /translation="MKLSDLLNYSSIYIQCHDNPDADAIASGYALYEFFKSKGKKVRL
                     IYGGPFKITKPNLKRMIEELKIPIEFAEELSLKDELLITVDCQYGAGNVKKFNAKNVA
                     IIDHHQQEIYDVAQAEIRSYLGSCSTLVWRMLLDEGFKVNDYKDVATALYYGLFTDTN
                     SFIEIHHPLDKDLRDNIYYDASLIRILKNCNFTLEDMEIAGIALIRHSYIDAYRCAIF
                     KVHPCDPNILGFISDLALQVDKIDTCIVYNETNAGVKFSVRSCIKEVMASEMAAFIAE
                     GIGSGGGHTDKAGGFISESKFRELNLGMNKDGYLHHRIKEYFESFDIIYSSHHKLDLS
                     TMEVYKKKNLVVGFVPSTDICEEGTPLLIRTIEGDLDIVASDDIYIMIGIKGEVYPIK
                     QEKFISSYKVLGEAFVLEGVEYFPLIKNKITGEYIDFRNYAKACMSTGEVFIYAKPLT
                     KNTKVFTNWDPGKYMSGKEGDYIAVRKDDYHDVYIIEKSIFNKTYQKCE"
     gene            complement(11798..>12011)
                     /locus_tag="PLG49_08330"
     CDS             complement(11798..>12011)
                     /locus_tag="PLG49_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOQ17362.1"
                     /translation="NWGVQYTPVANGKIYNNPSEESIRAARMALEGQNNVGECLYFLN
                     PKKSTNNWIIKNRTFYRRIGNHDFYL"
BASE COUNT         3937 a         2289 c         1813 g         3972 t
ORIGIN      
        1 caataaaata tagtatttcc gtattaattc ctgtattgct gactccaata ttatcaaact
       61 gtttgccaat acatacctta attttattaa ccacttctga atgtttttta ataataagtt
      121 tataatccgt tcggtctgta tcaaatgaaa ccaacttacc tgcattaatg tactggctat
      181 cggatttttc tattatttca ttttttgaat tcagaaaaag gattctaaat tcccttctgc
      241 ctgtagtagt taatcgaata attttttcat aagtaaataa ttcaggattc ccatcatttt
      301 ccacaggaat cataataaac ctatcatcat ctttaatatc atcaaacaat gcccagttcc
      361 aaccatttgg atttttgaat tgaataactg ctttgtcaat cctgtttttt gtcttaagtg
      421 ttattttata atctgtatcc tttatatgag taaaagtaat accctgtcct ccaacctttg
      481 tgaactttgg aataacaatt tctttttcta cagtctttga atctgtatta tccgctgtaa
      541 tagttacact atacttcgcc actttttctg accaggtcgg ctgaactttt actttccata
      601 ataaatcata cttttttgca cctgcgggta aatctcctaa atacatgctt tgactaccct
      661 ctactatttc cataccttca ggtaaattta aagtagcaat aacattcgtt gctgtacccg
      721 taccaatgtt ttcaataaat gccgatatag tcataggatt aggagaatac tcttcttctc
      781 cggcttcatt ttttaatact cccagctctg tagtgcccat tactgcaagg gacaaaggtg
      841 gtgttaaatc ttgttctaat ttaccaagac caaaaaatgt tttacaacta aaagacgcat
      901 ctttccccag agttctttca ttccaatata aagctacagc actatctcca ttactctgtc
      961 cctctggtac gtaataatcc cacatattaa gtcttgcact tgtccaattg gtaaagcgta
     1021 ctctgtcagg catgtattcc ttatcaactt ttaaaatccc ctgagcaatt attttaggat
     1081 tcgttactga attaaaaatc tgccaatatt ccggtatatc atccccccat agatctgttt
     1141 caattgctct ctctcctata ccaggtattt taaagggaac caggtcattc ccatcaaaga
     1201 ctgtatcaag cagaaggcgt acaccaaccg tatggggcgt atcacctgta tttttcactg
     1261 tatagagaaa ttcaacagta tcctctcttt gagtatatgg attatagaca atacttaact
     1321 gctgataaac aataatatct ccaatttgtt tagtaccaat cattttattc tcaaacgtcg
     1381 ttactgaatc cagattttca aagtcataat tttccccatc tattcttatc gttgccaatg
     1441 atgatagacc tgagccatac aataaatctc tataatcatc ataaggattg tcaggatcac
     1501 ctcctgtagt catcactcca tgaggaccat taacataggt aaatcttatg taatcattgc
     1561 ctattgatcc ttttttagct tctgatgctg cctgcaaacg aaaagaacga ctggaaacat
     1621 tattattctt tcttgccaat acttgttcct tccaaatttt atactctgtt tcatcttctg
     1681 gataatgatc aaaatcaatt tctccatcta agctaaaata atctggaaat tcaaagtttc
     1741 catcaagttt tgcttcaaat ataatacgta ttctttcacc tgcataaaaa tatgctgtag
     1801 aatctacccc gcttattgta tattgattat atctgcatct tacaatttcc gcattttcga
     1861 tatgaataat ttcttgattt aagttaaact ctaattccca atctttaaaa gaaacaagtg
     1921 atgtattttc taatatgatt tctccaataa aagtatcctc tgattcttct aaaatggtaa
     1981 aattcgcttc aaaaaagtca tctctaaaaa taacaggttg atcgacgatt aaactttttt
     2041 gtggcaattc ataaaccctc tcagctactg tttcatcttt tgcagcaggt ccttccgtgc
     2101 taagttcttc tgcaaaataa tcctttgagt cattaccaaa atcattatta actactattt
     2161 cctgactttc taattgagca tcatcactag cgtatgtcat ttgcggcatg gacgtcatca
     2221 tcattaccaa gattaaaaaa aagattttta gctgatttat ccctttcatc tctatcctcc
     2281 cattgactta cgaatttgtt tttattttaa tcttaaaaaa atttcaacat tttatttgta
     2341 attttttgtt atatttatgg tattcagttg ttttttattg gcatatttca ttaattattc
     2401 taatacactc ttctctttaa aatgacatta tggcttgtaa tacttaaaat ccaagtatta
     2461 gcgatatatc ttttaaaaaa ttcaattata atctcatgac taaggggcag ttaactgccc
     2521 cttaaatttt taatcccatt gctttcctct ttgacaggat atgagtttct atattattgg
     2581 ccacttctac cggatcatcc cctaaagcaa ttttacctcc tgttatactt tccacatctt
     2641 ctgttaacag ctttactaaa gcaggtgccc ctgttacaaa aggcgtagga gacaaatggg
     2701 tatacgcgcc ataagccact gcaaaaacgc catctatggt cgccttttgc tccatccatt
     2761 ctggtgctgt aacagcaatg ggtagatcgg atacatccac atcaaggtgg tctgccaaag
     2821 ctgaaaccag catagaaatc ctgcccgtat ctgtgcaagt cccaaaacta aggactggtg
     2881 gaatctttaa agcagaacat acttgtctca aaccctctcc ggctatattt aaagcctcta
     2941 aattacaaag accggctaat tccaatgcat ggttcccgca acctccgctg acaaccagta
     3001 tatccttttt gataagctct ttggttaaat tcactgtatt ccagtcctga ggtccgtttc
     3061 gtaaagttga gcaatttgca agggctacca ctcccttaat cttgcctgct gcgatgacat
     3121 ccactaaagg atctaactta ttgcctaaaa cttctaaaac cgcttctgta gaaaaacctg
     3181 caattgcctt ttgcgtgatt ttaggaaccc tgggaacaat ctttcctttt cttttcttaa
     3241 agttttcgat tgctaaatca attaattgat ctgccatctc tcctacttta tccggatgat
     3301 aaggaatagc atgctgaaga ccaggaattc caataatagt actgacactg actaaagtca
     3361 cctgatattt ttcagcatac atgtcaatgg caggcggaga acaattttct tccatggcca
     3421 taacatcgac ggttcctgtt gccagtaagg gttcaatagc cagccagttg cccataagcc
     3481 ctacgaaaac atcatctact tcaaatcttt gcaggagttc ctgacctgtc tcaattgaac
     3541 caactaccct taaaccttta gcacctgctg ctcttgcctt ttcctgaatc ttaggatccc
     3601 gtgctttttc aagaactgct acccctgccc agggttggtg cccgttaaat accacattga
     3661 cataatccgg gtccattata cctaaatcca ccgtaacttc atggggttta ggcgtaccaa
     3721 ataagatatc ttgtaccatc tccaaaccta tttgtgcatt atagattgtt gccagtccta
     3781 agcgaagtgc cttttttgcc aaagaggcat aatcaccgtc tacattggtc agacaactgg
     3841 ccacacaaat ttcttcttcg tgattaaccc ccgccggata taaatgtgtt tttttccata
     3901 attcttttct ttttttagat gcaaaaactt ctaccatgat attaggctca tccttgccta
     3961 ttttcatttg atgctctaaa aaatttgcca gatcaatagc catttgatta atgtcctgat
     4021 tggtatctat tcctaatttt tcacacatcc attttaattt atcaacatct gtaattttaa
     4081 aaggggtttt cccttctcct gttgccctga gggttctgaa agcctcgtag gcatggtggc
     4141 tataagtccc tgctcccata atattttgaa gaagtaaatt ccgcattgcc atagcatctg
     4201 caccaatacc acaaacccct ctgtcctgcc ctcctttttc actgatccgg catggaccct
     4261 gggaacaaag ttggcaactc aatccctgaa gacaaaattt acaccttatt ttttcctgta
     4321 atgtccatcg gtcaaaaaca ttggacattc catcttcacg gattcttttt aacatctctt
     4381 ccaccgaatc atgatagctc actctgcctt cagtcttttc caaaatggtt tcatccataa
     4441 ctttattctc ctttctttgt atattgaaga ttttttccta aaaatcgttt aatatacata
     4501 gaaaactact cttaaattaa gagcaggaat tttttaaaac cattgtaaac aatatgaatt
     4561 ttgtatataa tataaaatag tttaatcatt tataattaat aattttttaa tgaggtgaaa
     4621 aaatgagttt tatttttatt tccagttcat caaatgataa agcaatagcc gatgcaatat
     4681 atgaaaatct cgagaaaaaa ggtataaaat gctggaattc tgataaaaac attcatgcta
     4741 atcagaatta caatgcagca atactccagg caattcataa ctgccgtgct atggttttta
     4801 tctattcatc aaatgccgaa tcttcacagg atgttttacg tgaagtcaaa gaagcttcgg
     4861 aaaacaataa aattattatt ccgtttatca ttgaaaacaa aataccttct caaaatatgg
     4921 aagcctatct gcaaagcaac tataaaatcg atgcaacagt aacttctata tatgaaagca
     4981 tatacaaact tgaaaattta ttaaaaaatc ttttagccgc acaaagaaat aatgttcccc
     5041 atgctgtaat accacaagca cctgtagatt acagcaacaa ctttaattcc cctaatccta
     5101 accgtcctgt cccggcaaaa aaaagtaagg cacctttaat tattggaata tcagctgcag
     5161 ccgttgtgtg tctgattctt ttagcaggag gcatctttgg catgtcactt cttttggctc
     5221 ccacggatga atcttatata gaagaaataa caacaaccag cagtaacggt gcttatgata
     5281 ttggttttaa agttgaacag gatgatgatt tgtatggaag cagatttaca gaagaagaaa
     5341 tcatagaatt cgcaaaagaa gccatccggg aacttgaccg tacaaacagc gactcagaat
     5401 tttatcaaaa ttacggatat cagggaccag aatacaaaga tttatataat gttcaggaag
     5461 atgttctgtt tgccatgatg ggcttagaag ttgtccgatt tgatgatgta aaagtaatgg
     5521 gaaaaaataa aaaggatttt ggtattgagt atcttttgag tatggatttt accgtttatg
     5581 ctaactatga cagggaaaca gaaactggag aaacagagca tatagaaggt gaagcctctt
     5641 tatataataa tgtcgttatt attgaaacaa atgacggtga attaaaatac ctttacatgg
     5701 aatccatcaa acaagaagaa ctggaaaaca gatatgaaaa aattaattct ttctatgaag
     5761 aaatgaaaga aaaagaagtc attgaagcag aagataccgt tcaagcttat gttccttcag
     5821 atgctaatat caacaacaat tctttaaaat ctgtaaaaga aattgttgaa gaaaacgatc
     5881 acaaagttgt tgctgttttt gttgacacac cagaaggaga agtacaggga agtggctttt
     5941 tcattgaaga cggtatcatt gtaaccaatt ttcatgtcat cgaaggtgga agaagtgctt
     6001 atgtccgcct tacagacgaa agattcattg aagttgaagg aattgtatgt gctaatcctg
     6061 ctcttgattt ggctatttta aaattaactg aacaaattgg tgttgaaccc gttagattag
     6121 gtggaatcag cgatgttcaa aaaggtgaaa tggctgtagc aataggctca ccactgggac
     6181 tttttaatac agtatcaact ggaattatat caaatacctg gaaccaggat ggtgttcact
     6241 taattcaaat ttctattccg attactcatg gtaattctgg tggagcctta tttgatgatt
     6301 ccggtaaagt gattggcgta acgacttctg gtattggtga tgctaatttg aactttgctg
     6361 tttcaacaga acatttactg gaagtttgtg aattaatcaa agaaattggc tatgataata
     6421 tagaagttgt tcctttcaaa tgaaaattgt ttattgctca tattagaccc agcaaaacat
     6481 ttagcagtgg ctaaaataag ataagcaggt gatttaatgc caaaatacga tgctttcatc
     6541 agttatcgtc atactgaacc tgataaaaca atcgctgaaa aacttcataa aatgttagaa
     6601 acctataaag taccttctaa aattgctaaa aaaacgggga aaaagaaaat taaccgtgtt
     6661 ttcagagaca aggaagaact tcctacatcc agtaatttag ctgccagtat agaagatgct
     6721 ttaataaatt ctgaatacct gattgttatc tgttctccac gaacgcctga atccaaatgg
     6781 gtattaaaag aaattgaaac cttttcaaaa cttcatggac atgataaaat tctggcactt
     6841 ttgattgaag gagagcctat tgatgctttc cctgaacaac ttcgctttgt tacaaaagaa
     6901 atgattgata atgaaggaaa tgtcattgaa gaaaaagaag aagtagagcc ccttgcagcg
     6961 gatatacgag gtaaagatac aaaagaatta atcaaaaatt taaaaaaaga attattaaga
     7021 ttactggcac ccattttagg ctgcaacttt gatgatttaa ggcaaagaca cagggaaaga
     7081 tttataaaaa gagtgattac gatttccatt tgcttctcct tgttcttcct tgcctttggt
     7141 tcgtttagta catggcaggc catacaaata aaacaacaag taaataaaac ccttgaaggt
     7201 caatccttat acttagcaaa tttatcccga caagtgctca atgaagggga tagaagaact
     7261 gctattcttt tagctttgga agccctcccc aaaaatcttg aaaaacctga acgtccttat
     7321 gtagaagaag ctgaatatgc cctgactgaa ggattgaata tctacaatac aagcaatact
     7381 tttatgagtg atattgtatt gaatcatcga aaaagtgtgt ccaatattca gatcagtcca
     7441 gatcattcta aagtgttcac tgtctgcaac gatgggaaaa tctatttatg ggacacaagt
     7501 aatggtaatt tgctttatac tttcccccat aattccatca cgctttccaa agaacaaagt
     7561 tctttttctg ctaacggaga aataattgta tcgattactt cagatggact ggctgcctgg
     7621 agtacaacaa caggcgtaaa gctttgggaa atagaagcta ctattcctcg ttttgtatta
     7681 aatccagatg gaaaaagcct tgcattgatt caacaagaac caacgaaaaa ccatttaaaa
     7741 ttaattgata tcaacaatgg aaacgtattc atccatatga ctttcgaaag aaaaaatttc
     7801 gaacttgtga atgccatggc ttttaaccaa gatggatcca tccttgcttt ggggacagat
     7861 cagggcttaa tccaattagt agatatccaa aatcaaaaaa atctttatcg tcttaattct
     7921 gccaatgaaa atattaaaaa agtctttttt agtgatgacg gtaaaaaaat agctgccttc
     7981 tcaggaaatt ttgatttaga aacggcagat atttatagta ccggcacagg taatatagaa
     8041 atatgggata ccgagagtac tcaaaacatc ttaaaccata aattaaacac ctccaccatt
     8101 gctgaagcaa gttttagccc tgtaaactca aatattgttg cctatacaga aaatgaattg
     8161 gttaaagtcg ttgatatatc ttctggagaa atcttattta cttttaccca tggttctaag
     8221 gtaacaaact ttaaaatatt caatgatggc gacttttttg ttactacatc cctagacgga
     8281 aatattatgt tttactctct tgacataggc attagtctgc cttcctggaa tatttatcat
     8341 aatgattcca tatccgtaat ggatatagag gataatatac ttgcactatc tgcctctact
     8401 tccaataaag cttatttatt aaaatcttta gataatcctt atgtaacaac aatgagcgga
     8461 catagttcaa caatcgaaaa aggtaaattt tcacccagcg gccagaaggt tttaacagtt
     8521 gcagaatcag aagtcttcat ttgggatatt gaaaagaggt ccttatttaa taagcttaat
     8581 ggaatagaag gaactatttt gaatgcagat tttacacctg atgaaagcaa aatatctatt
     8641 ctgactaaag agggatatat taaaatcttt gatatgtcct ccggctcaga attatcctct
     8701 ctaaatacag gttcctttac aaattattac tatagccctt ccaactcttt agtggcatta
     8761 caaattgacg atatggttaa aatatttgat tgctataact gtaaagagct attgactatt
     8821 aataataaca gttatttaaa taaaagtttc ttttctaacc aggataagtt cgttttaatc
     8881 ggcaaatggg acggagaatt ttcactttat gatataaaaa cagctaagat cctacatact
     8941 tttcaaaaca aagaaaacct caaaataaat gtaatcgctt ttaataaaga tgatagtctg
     9001 ctggctctta atcaaggtga cagaatctat atttacgata cgaaaaactt tgaaatggtt
     9061 ggttacattg aaaacttatt gttcgccccc tcagtcatta catttagtca tgacaaccgt
     9121 gaactatttg tcggcttaga tgataaaagc attcaaattt acgatttaga aactggacag
     9181 tataagacag aacttacagg acataatcaa attttaaaag aatgcaggta tagtcctgat
     9241 ggaaatctgc ttataaccat tggtgaattt ggagaatgca tattatggaa tcctgtgaac
     9301 tataaaaaat tagcttatat caataaagta cttgatatta atcattcatt tacaaatttc
     9361 ttatccttca attatcaaga cgtcatttta atgccgcggt atgatttaga aagtctcgta
     9421 cgtattgcaa aaagtgaatt gggaaatcgc aaactgagca aggaagaaaa aatcaaatat
     9481 tatattattg aatagtgacc taaaaaaata cttccagcca gatagtctct atttgtgctg
     9541 gaagtatttt tttgcttttg gttacaataa gacttcaaaa tttattttaa tgattacggt
     9601 ggtgatcttc atcatgaatc acctggtcaa taacaatgac aacggccaaa atcaacagat
     9661 cattttcatc gtttacaatg tctacgccat aagaatctct aaaagaaaag aattttttac
     9721 ttacatgggc aaccagacgg ctatttttca taattctaaa atcatagcca aaaaaatcgc
     9781 cttcaacact atatgaacca aaaggactct ctatctgaaa tttaggtcgt aaaagggaaa
     9841 aagcttgttt tacttttgca gcaaaattcc cgtcgaaata aagataatac tcaggtaaca
     9901 gtttaaataa tttttgttca atataaattc tttcctgccc attactatca taaatcttaa
     9961 gtttatcccc tagggaaaaa accttacctt ctacaaaata cctttctgaa ccatattcat
    10021 cttttataac aaatttatct ccaatactaa atattttttg attgattata tatttcatgg
    10081 tatactcctt tattcacact tttgataagt tttattaaaa attgattttt cgattatata
    10141 gacatcatga taatcgtctt ttcttacggc aatataatct ccttcttttc ctgacatata
    10201 tttacccgga tcccaattgg taaacacctt tgtgtttttt gtcaggggtt ttgcataaat
    10261 aaagacttct cctgtagaca tacaggcttt agcataattt ctaaaatcaa tatattctcc
    10321 tgttatttta ttttttatta aaggaaagta ttccacacct tctaaaacaa aagcttcacc
    10381 caatacttta tagctgctta taaacttttc ctgcttaata ggatagactt ctccctttat
    10441 cccaatcatg atataaatat cgtctgaagc aacaatatcc aaatcgccct ctatggttct
    10501 gataagtaag ggggttcctt cttcacaaat atcggtagaa ggaacaaagc ctacaacaag
    10561 attcttcttt ttataaactt ccattgtact aagatccagc ttatgatgac tactgtaaat
    10621 aatatcaaag ctttcaaagt attccttaat cctatgatga agatagccgt ctttattcat
    10681 tccaagattt aattctctaa atttactttc actgataaag cctcctgcct tatccgtatg
    10741 tcctccacct gaaccaatac cttcagcaat aaaggcagcc atttcagatg ccataacttc
    10801 ctttatacaa ctgcggactg aaaacttaac cccagcattg gtttcattat aaacaataca
    10861 tgtatcaatt ttatccacct gtaaagctaa atcacttata aatcccagta tattcggatc
    10921 acaaggatgt accttaaaga ttgcacaacg ataagcatca atataagaat gcctgattaa
    10981 agcaatccct gctatttcca tatcctctaa agtaaagtta cagtttttta atattctaat
    11041 taagctggca tcataataaa tattgtctct taagtcttta tccaatggat gatggatttc
    11101 aatgaaactg ttggtatcgg taaacaggcc ataataaagt gctgtggcta catctttgta
    11161 atcattcact ttaaaacctt cgtccaaaag catcctccat actaaagttg aacaacttcc
    11221 aagataagac cggatttctg cctgagctac atcatagatt tcctgttgat gatggtctat
    11281 aattgccaca ttttttgcat taaacttttt cacatttcct gccccgtatt gacaatctac
    11341 cgtaatcaaa agttcatcct ttaaagataa ttcttctgca aattcaatag gtatctttaa
    11401 ttcttctatc attcgcttaa gattgggttt tgtaatttta aaaggtcctc cgtaaatgag
    11461 tcttaccttt ttgcctttgc tcttaaaaaa ctcatacagg gcataaccgg aagcaattgc
    11521 atcagcatcg ggattatcat gacattggat ataaatggaa ctataattta ataaatcaga
    11581 aagtttcaag ttaatccccc taaatcaaat ttttcattat cttacgaaat atttttacaa
    11641 tatttgtatt ataatcatta taataaagcc taaacaatat gtctataatt agaggaaata
    11701 attcattttt tataaaaaaa atactcccaa aaagatagcc ttaaaaattt gactatctat
    11761 tttggaagca aatatataat aagcttaaag cgtggcttca cagataaaaa tcatgattac
    11821 caattctgcg ataaaaagtc ctgtttttga taatccaatt gtttgtggac tttttgggat
    11881 ttaagaaata aagacattct ccgacattat tttgtccttc caaagccatg cgggcagctc
    11941 ttatactttc ttctgaagga ttattatata ttttcccatt agctacagga gtatattgta
    12001 caccccaatt g
//
LOCUS       DAOSUD010000065        11956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3063_length_11956_cov_25.460802, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000065 DAOSUD010000000
VERSION     DAOSUD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_11956_cov_25.460802"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLG49_08335"
     CDS             complement(<1..778)
                     /locus_tag="PLG49_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HOQ17363.1"
                     /translation="MKSLCSEVVRGFGYQLKSYNAFRNLYICTTDSGIKVIKSVENKI
                     PSLLFQHSVKEHLYQNGFKYLDRFHLSLDKTPYYAYQGNIYVMTDWLEGRECDFNHLE
                     DIKKAISTLADMHKLSKGLVPVEGSFAKPYDEDLSFVIKKRIREFHTIKKKIKRQSHM
                     SNFDLLFIKNYKKYEEYSQRALEWLEKMDYHQIKAKVEKEKYFYHNDYTYHNLILVPD
                     GSLFVTHFEQCRYGLPVYDLIYILKKIMKKHKWDIKIASEL"
     gene            980..1933
                     /locus_tag="PLG49_08340"
     CDS             980..1933
                     /locus_tag="PLG49_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17364.1"
                     /translation="MIEVSNLSKSYGSLKAVDNISFSLKEGEILGFLGPNGAGKTTTM
                     NMICGLMIPDEGEIKINGINLLENPKKAKRHIGYLPEIPPLYPDMTVMELLSFCCQLK
                     DYNGNIQEECQRVISLCGIDNVKTRLIKNLSKGYRQRTGIATALIGNPKVLILDEPTV
                     GLDPKQIKEIRDVIKSLSKKHSIILSSHILPEVNALCDRIVIINKGKIAAIDTPDNLS
                     YSLQSSFNLYLEIEGPKEDVLYTISSLNNILSVEAVKQIDEKIFGYVVKGEKDKDIRR
                     SLFYALADGKMPMLKLEVEKMSLEDAFLHITLEEDLREGEE"
     gene            1939..2646
                     /locus_tag="PLG49_08345"
     CDS             1939..2646
                     /locus_tag="PLG49_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17365.1"
                     /translation="MKTWVIFKRELRGYFYSPQAYILMGFFILLTGFFFSDYIINTRY
                     PSIQYVMSSWPMIFLFIAPILTMKLFTEERKYGTEKLVFSSPVSVTEVVLGKFFSVCV
                     VYLASLILTLTHVIIVFIIGNPDLGRIFTAYIGNILLGFSFLSVGLFASSLSENQIIA
                     AIISFGLLIFFWVIDILQDIFTGIPHKIIQIISLFEPYAGFQNGFISLSSVVYYISFT
                     GIFLFLTVRVIKSRWLQ"
     gene            2661..3986
                     /locus_tag="PLG49_08350"
     CDS             2661..3986
                     /locus_tag="PLG49_08350"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOQ17366.1"
                     /translation="MNRKKLSRNIKYSSNSFIFTAAVIGIVIIINLIIRSFHIQWDLT
                     SNKIYTLSEEGINALKKLDQDVSILFFANPADPVTSDIQQLYENMATVTNHISLEIID
                     PLKNPSLVNQYQITTEYSAVVSSEKMSKQVHGFDIIRTDYSTGEYFFDGEGALIQAIL
                     DVTADKQTQLYVLEGHGEFSLDENLTLFKYLLEQKGVKVHSFTLTTSADLPGDSDIIC
                     ILGPLYDYTPNEIEILKDFLDKGGKVILAFNDYQENENLDNLKNFAQNYGIKVENIPL
                     SDSLRHYAQDERALVPVYTSHEIVQRLEEDNIATIIPNARKLSIEKKEDIINRVVLET
                     SQEEAIAITAEKYLDNDETAQLLVIGNAFFLHDQAISLGGNSDIVLGALTWFKDDESI
                     VNIKPKTYSPEPIVLVGIQSQLIFLVCVVLIPLFVFIFGLMLYLRRRTL"
     gene            3983..4912
                     /locus_tag="PLG49_08355"
     CDS             3983..4912
                     /locus_tag="PLG49_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOQ17367.1"
                     /translation="MKKIIPTLLAVIIFLAAYAYYCYYEKTYEVPEDETIYVWQLEKN
                     KITHIDITHENQSIQLVRDGQSWNITQPIEFPADDFNVNTLLGRFNTLQATAFVEENS
                     SHLSNYGVDSSKTSITFTDDEGNSNTLILGNEAPLSKGIYVYNKNNNSIYTISSRLLE
                     DLSFDLDFFRDKILLSFKKEYIEKIKIENEGKTFEIQLKDDQWYSNDRTLDEASVRSL
                     ITVLNTRKISEFVKDNASEKDLEKYGLKNPRTSITIYLYDSDNSTLNLYLGNIEDTFT
                     YASRDKKFIYKISSSSLLSNDLSVENFYIKNKE"
     gene            complement(4977..6314)
                     /locus_tag="PLG49_08360"
     CDS             complement(4977..6314)
                     /locus_tag="PLG49_08360"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOQ17368.1"
                     /translation="MYKEKIPKEVEEIILKLNKEGFEAYIVGGCVRDLLMGRIPNDWD
                     ITTSAKPEEVKKIFPKTYDTGIAHGTVTVILNGAHFEITTYRIEGKYEDYRRPKEVTF
                     TRNLEEDLSRRDFTMNAIVYHPQEGFMDPVEGIKDIKNKMIRCVGEPSKRFEEDALRM
                     LRAIRFSAQLDFEIEEKTLGAIKGHAALITHISAERIKDELDKMLMASYTDRLILLYE
                     TGLLKYILPELDKCFAVEQNHPHHVDNVGIHTLKSVGLVDKNLNLRWTMLLHDIGKAN
                     YKETDEKGIDHFHGHVRESVRLTKDIVKRLKFDKKSSSHIIKLIDWHDERIKKDKRAV
                     KEVLSQIGEEAFLDLLKVQLADSRAQNPKYIKEKEEQLQSLYEVYKEIKENNECFLIK
                     DLAVNGEDLIRLGIKEGKEIGELLKQLLELVIENPDQNNKESLLLYVKENLIP"
     gene            6413..6886
                     /locus_tag="PLG49_08365"
     CDS             6413..6886
                     /locus_tag="PLG49_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HOQ17369.1"
                     /translation="MDKTVIALFNRREDAEQAANELRNQNFPIENISIAVKESDNNEK
                     QEELVDDDITDGITAGGVLGGIAGLLIGAGTFAIPGLGLLTAAGPLAGLLSGAVTGGI
                     VGGLIDLGIPKTKTSLYEEGLKEGKILMTTKVKAENINQVMNILKKYGGNEIESY"
     gene            complement(6909..8345)
                     /gene="proS"
                     /locus_tag="PLG49_08370"
     CDS             complement(6909..8345)
                     /gene="proS"
                     /locus_tag="PLG49_08370"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOQ17370.1"
                     /translation="MEKDKKFVQEITDMNEDFAQWYTDVVKKAELIEYSSVRGCMIIQ
                     PYGYAIWELIQSQLDQRFKETGHKNVYMPLFIPESLLQKEKDHVEGFAPEVAWVTHGG
                     NEALTERLCVRPTSETLFCEYYSNVIQSYRDLPKLYNQWCSVVRWEKTTRPFLRTLEF
                     LWQEGHTAHATAEEAQEETIRMLNVYAEFCENVLAIPVIKGKKTEKEKFAGAKETYTI
                     ESLMHDGKALQSGTSHNFGDGFARAFDIKYTDKNNELQYVHQTSWGVTTRLIGAVIMV
                     HGDNSGLVLPPRIAPIQLVIIPIAQHKEGVLEKAHEIKERLSSIVRVELDDSDKMPGW
                     KFSEYEMKGVPFRLEIGPKDIENNQVILVRRDTREKIKVAMDELEIKIPELMDTLQKD
                     LLEKARQMRDSKTYIAKNMDEFKDIITNTPGFIKAMWCGDEACEIKIKEETGATARCI
                     PFEQEHLSDTCVCCGKSADKMVYWGKAY"
     misc_binding    complement(8445..8644)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8710..9150)
                     /locus_tag="PLG49_08375"
     CDS             complement(8710..9150)
                     /locus_tag="PLG49_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOQ17371.1"
                     /translation="MLIKQLSVFLENKSGRLTEVTKTLGDLDINISALSIADTAEYGI
                     LRMILTDPDKAYKALKEKGFSVRLTDVLCIATPHRPGELAKALDILSKNGISVEYMYA
                     FEVGEKAFIVIKTADNQKTIDTIIAHKDEVELINADEICQIRAK"
     gene            complement(9162..10460)
                     /locus_tag="PLG49_08380"
     CDS             complement(9162..10460)
                     /locus_tag="PLG49_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOQ17372.1"
                     /translation="MIWNEYNECMSREEMIKLQSKRLCETVERVYHNVPFYRKKMQEM
                     GMEPGDIRGIEDLNKLPFTTKQDLRDNYPFGLFAVPMSEVMRLHASSGTTGKPTVVGY
                     SRKDITTWSEVVARCLCAAGVRKNDVIQIAYGYGLFTGGLGLHYGSEKVGATVIPISG
                     GNTQKQIQIMRDFKSTVLACTPSYALYLAETMEEMGVDPKELSLRVGIFGAEPWTEEM
                     RKKIESKLHLKAIDIFGLSEIVGPGVSCECEMQNGLHINEDHFIPETINPDTLETVSE
                     GEVGELVFTTITKEALPLLRYRTRDLTVLHYDNCPCGRTLVRMEKCKGRSDDMLIIRG
                     VNVFPSQIESVLLNMGETKPHYLLIVDRINNLDTLEVWVEVDEIFFSDEIRNLESLSA
                     KIKHQIESTLGISVKVKLVEPKTIERTEGKSKRVIDRRKL"
     gene            complement(10672..11739)
                     /gene="ytvI"
                     /locus_tag="PLG49_08385"
     CDS             complement(10672..11739)
                     /gene="ytvI"
                     /locus_tag="PLG49_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOQ17373.1"
                     /translation="MIEFYKKHKDSINKLLTALFLILCLYLFFRYIFSYVAPFIIAWL
                     LASAMEPLVRWFQKKCKMPRGLASILSIIIILVFIGTVVTGIVMRIIQEAQAFAQDFP
                     AYYKGFMESLESLRGKTETFILALPDSLQDLIRNGFDAIMKSLTEAVGPGVKKGSIGF
                     VVALPNAFFVSVVTLIATFFMSKDKMKIQNFIERQLPASWNRKIDIARRDLFGALLGY
                     IKTQLILMIFVSSICIIGLTILRVKYSLLIGFVIGIFDAFPILGSGGILIPWAIYNFI
                     TGDYFRAIGFLIIYGVVILFRQMVEPKVLGTQIGVYPLVTLMAMYIGIKMFGVIGIIA
                     GPIVVIFIKTMQKVGIIPEWR"
BASE COUNT         3865 a         2287 c         1734 g         4070 t
ORIGIN      
        1 gcaattcact ggctatttta atatcccatt tatgcttttt cataattttc tttaaaatat
       61 aaatcaaatc atagacagga agaccatatc tacattgttc aaaatgggta acaaataagg
      121 aaccatcggg aactaaaatt aaattatgat atgtataatc attatgataa aaatatttct
      181 ccttttctac tttagccttt atctgatgat aatccatttt ttcaagccat tctaaagctc
      241 tttggctgta ttcttcatat ttcttataat tttttataaa taataaatca aaattactca
      301 tatgactttg tcttttaatt ttcttcttta tggtatgaaa ttctcttatt ctttttttaa
      361 taacaaaaga taaatcttca tcataaggct ttgcaaaact tccttccacg ggaacaagcc
      421 ctttggacag cttatgcata tcggctaagg tagaaatagc ctttttaatg tcttccagat
      481 gattaaaatc acattccctg ccttccagcc agtcagtcat cacataaata tttccctgat
      541 aagcataata aggtgtttta tccaaagaaa gatgaaaacg gtccagatac ttaaaaccgt
      601 tttgatataa atgttctttt acactatgtt gaaacagtaa acttggaatc ttattctcta
      661 cagacttaat aacttttatc cctgaatcgg tcgtacaaat atataaatta cgaaaagcat
      721 tataagattt taattgataa ccaaatcccc ttacgacttc agaacataaa cttttcaact
      781 tatcaccacc attctatata aaaagcaatt ctttagataa aaataatcca ataaaaattg
      841 actccctata tatttatgaa aaactatgga gaaatatgaa gagatgggca tctattttta
      901 ctttttgtaa tttgttcata atttgttcat agtctcaaat tatactataa ataattatta
      961 gttaaagagg tgttcttcca tgattgaggt ctctaatctc tcaaaatcct atggctccct
     1021 aaaagcagtt gacaatatta gtttttctct taaagaaggc gaaattttag gttttcttgg
     1081 ccccaacggt gctggaaaaa caacgaccat gaatatgatt tgcggtctta tgatacctga
     1141 tgaaggggaa attaaaatta atggaattaa cttattggaa aatccaaaaa aagcaaaacg
     1201 acatataggg taccttcctg aaatccctcc tttatatcct gatatgaccg taatggaact
     1261 cctttctttt tgttgtcagc taaaagatta taatggcaat attcaggaag aatgtcaaag
     1321 ggttatcagt ttatgcggca tagataatgt aaagacaagg ctgataaaaa acctttccaa
     1381 agggtacagg caaagaactg gcattgcaac cgccttaatc ggaaatccta aggtccttat
     1441 tttagatgaa ccaactgtag gtcttgaccc gaaacaaata aaagaaatcc gggatgttat
     1501 taaaagtctt tcaaaaaaac attccatcat tttaagctcg catattcttc ctgaggtaaa
     1561 tgccctctgt gacaggattg tcattattaa taaaggaaaa attgctgcta ttgatacacc
     1621 tgacaattta tcctattctc ttcaaagctc cttcaatttg tatttggaaa ttgaaggacc
     1681 gaaagaagat gtcctttata cgatttcttc tttaaataat attttatcag tagaagctgt
     1741 caaacaaatt gatgaaaaaa tctttggata tgtggttaaa ggagaaaaag ataaagatat
     1801 ccgccgctct ttattttatg ccctggcaga tggaaaaatg ccaatgctta aattagaagt
     1861 tgaaaaaatg agcctggaag atgcattctt gcacattact ttagaggaag acctcaggga
     1921 aggagaggag taaggcttat gaaaacttgg gtaatcttta aaagagaatt aaggggctat
     1981 ttttattcac cacaagctta tatcttaatg ggatttttta ttttactgac aggttttttc
     2041 tttagtgatt atatcatcaa tacaagatat cccagcatcc aatatgtcat gtcaagctgg
     2101 ccaatgattt tcctttttat tgctcctata ctgaccatga aactctttac agaggaaaga
     2161 aaatacggaa cagaaaaatt ggttttttct tcaccagtat cggttacaga agtcgtttta
     2221 ggaaaatttt ttagtgtctg tgttgtttat cttgcttcat taatacttac cttaacacat
     2281 gtgattattg tatttatcat aggaaacccg gatctgggac gcatttttac cgcttacata
     2341 ggtaatattc ttttaggctt tagcttttta tctgttgggc tttttgcttc ttcattatca
     2401 gaaaatcaaa ttatcgcagc tattattagc tttggacttt taatattctt ttgggttatt
     2461 gatatcctgc aagacatttt tacagggata ccccacaaaa tcatccaaat cattagtctt
     2521 tttgaaccct atgccggttt tcaaaatgga tttatttctc tgtcatctgt cgtttattac
     2581 atcagtttta caggtatatt cttattttta accgttaggg ttattaaaag tcgatggcta
     2641 caataatagg gggaaatatt atgaatagaa aaaaattaag cagaaatata aaatacagca
     2701 gtaattcatt catttttact gcagcagtca taggaattgt tatcattatt aatttaatta
     2761 tccgttcctt tcatatacaa tgggacttaa ccagcaataa aatatatacc ctttcagaag
     2821 agggtatcaa tgctttaaaa aagctggatc aagatgtcag tatcttattt tttgctaatc
     2881 cagcagatcc tgtaacttca gatattcagc aattatatga aaatatggcg acggttacaa
     2941 atcatatctc cttagaaatc atcgatcctt taaaaaatcc cagcttagtc aaccaatatc
     3001 aaattactac tgaatactca gcagttgttt cttctgaaaa aatgagcaag caagtgcacg
     3061 gctttgacat tataagaacc gattattcaa ccggagaata cttttttgac ggtgaaggag
     3121 cccttattca agccatttta gatgttacgg cagataaaca aactcaatta tatgttcttg
     3181 aaggccatgg ggaatttagt ctggatgaaa acttaacact ttttaaatat cttctggaac
     3241 aaaaaggagt aaaagtgcat tcttttaccc tcaccacttc agcagatctt cccggagaca
     3301 gtgatatcat ttgtatatta ggccctctgt atgactatac gccaaatgaa attgaaatat
     3361 taaaagattt tctggataaa ggcggcaaag taattttagc ctttaatgat tatcaggaaa
     3421 atgaaaatct ggacaatctt aaaaactttg cccaaaacta cggaataaaa gtagaaaata
     3481 tacccctgag cgactcttta agacattacg cccaggatga aagagccctg gtccctgtat
     3541 atacaagcca tgaaattgtt caaagactgg aagaagataa tattgcaacc atcataccca
     3601 atgccagaaa actgtctatt gaaaaaaaag aagatatcat taaccgtgtt gttcttgaaa
     3661 cttctcagga agaagcaatt gccataaccg cagaaaaata tctggataac gatgaaacgg
     3721 cacagctttt ggttattgga aatgcctttt tcctgcatga tcaggccatt agcttaggcg
     3781 gaaacagtga tatcgtttta ggcgctttaa cctggtttaa agatgatgaa tccatcgtta
     3841 atattaaacc aaaaacctat tcccctgaac caatcgtttt agtaggtatt caaagccagt
     3901 taatcttctt ggtttgtgtg gttcttattc ctctttttgt attcatattt ggcttaatgt
     3961 tataccttag gaggagaact ttatgaaaaa aattattcca accttattgg ccgtcattat
     4021 atttttagct gcttatgctt actattgcta ttatgaaaaa acatatgaag tccctgaaga
     4081 tgaaacgatt tatgtatggc aacttgaaaa gaataaaatt acccatatcg atattaccca
     4141 tgaaaatcaa agtattcaat tagtaagaga cggtcaaagc tggaacatta cacagcccat
     4201 agaatttcct gcagacgact tcaatgtaaa taccttactt ggcagattta atactctaca
     4261 ggcaacagct tttgttgaag aaaacagtag tcacttatcc aattatggcg ttgattcatc
     4321 taaaacaagc attactttta ccgacgatga aggaaattca aataccctga ttttaggaaa
     4381 tgaggcccct ttatcaaaag gcatttatgt ttataataaa aataacaata gtatttatac
     4441 cataagctca agactattgg aggatttatc ttttgacttg gactttttca gggataagat
     4501 tctactatcc tttaaaaagg aatatataga aaagattaag attgaaaatg aaggaaaaac
     4561 ttttgaaatc caattaaaag acgatcagtg gtacagtaat gacagaacat tagacgaggc
     4621 ctctgtcaga tctctgatca ctgtattaaa tacaagaaaa atttctgaat tcgttaaaga
     4681 caatgccagt gaaaaagacc tggaaaaata cggcctcaaa aaccccagaa ccagcataac
     4741 catttattta tatgacagcg ataacagtac tttaaatctt tatcttggaa atatagaaga
     4801 tacttttacc tatgccagca gagataaaaa atttatttat aagatttcca gttcttcctt
     4861 actatccaat gatttatccg tcgaaaactt ttacatcaaa aataaagaat aagatacctg
     4921 aaataaaaaa gctcctattt agaaaaaata acttattttc taaatgggag cattttttaa
     4981 ggaataaggt tttctttaac atataaaaga agactttctt tattattttg atctggattt
     5041 tctatcacca attccaataa ctgctttaaa agctctccga tttcttttcc ttcctttatc
     5101 cccaaacgaa ttaaatcttc tccattgaca gctaaatcct ttattaaaaa acattcatta
     5161 ttttctttta tctctttata aacttcataa agactttgca gttgttcttc tttttctttt
     5221 atatatttag gattttgagc ccttgaatct gccaattgta cttttagcaa atctaaaaaa
     5281 gcctcttctc ctatttgtga taaaacttct ttaactgccc ttttatcttt ttttattctt
     5341 tcatcatgcc agtctattaa tttaataata tgactgctgc tttttttatc aaactttaac
     5401 ctcttcacaa tatctttagt caaacgaaca ctttccctga catgaccatg aaaatgatca
     5461 atgccctttt catctgtttc tttataattg gcttttccga tatcatgtaa gagcatagtc
     5521 catcttaaat ttaaattttt atccacaagt ccgaccgatt ttaaagtatg aatccccaca
     5581 ttatccacat gatgaggatg attttgctca actgcaaaac acttatccag ttcaggaaga
     5641 atatatttta atagtcctgt ttcatataat aagattaagc ggtcagtata agacgccatt
     5701 aacatcttat ccagttcatc ctttattctt tctgcactaa tatgcgtaat taaagcagca
     5761 tgccctttaa tcgctccaag ggtcttttct tctatttcaa aatccagttg tgcagaaaaa
     5821 cgaatggctc taagcattct aagggcatct tcttcaaatc tttttgaagg ctctccaaca
     5881 catctgatca tcttattttt tatgtcttta ataccttcca caggatccat aaaaccttcc
     5941 tgaggatgat aaacgatggc attcatggta aagtctcttc ttgataaatc ttcttctaaa
     6001 tttcttgtaa aggtcacttc tttaggtctt ctgtaatctt catattttcc ttctattcta
     6061 taagttgtta tttcaaaatg agccccattt aaaatcaccg taactgttcc atgagcaata
     6121 cccgtatcat acgttttagg gaaaattttt ttaacttctt caggttttgc agaagttgtt
     6181 atatcccagt cattcggaat tctacccatc agaaggtctc ttacgcatcc cccaacaata
     6241 taggcttcaa aaccctcttt atttagtttt aaaataattt cttcaacttc tttgggaatc
     6301 ttttccttat acacatcttt ccctccaaat gcaaaagcga ttatttttat taaatcaact
     6361 ttatgaataa aaatgacttt ttaacttaaa ctatggaaaa ggaggtatct ttatggataa
     6421 aaccgtcatt gctcttttca accgcagaga agatgctgaa caagcagcaa atgaacttag
     6481 aaatcaaaat tttcccattg aaaatatttc tatagccgta aaagaatcgg ataataatga
     6541 aaaacaagaa gaattggttg atgatgatat taccgatgga attacagctg gaggggtgct
     6601 cggtggtatt gcagggcttt taattggcgc tggtactttt gctatccctg gtcttggcct
     6661 gttaaccgct gccggacctt tagccggtct tttatcagga gcagtcactg gaggaatcgt
     6721 cggaggttta attgatctgg gcatacccaa aacgaaaacc agtctctatg aagaaggatt
     6781 aaaagaaggc aaaattctta tgacaacaaa agtcaaagct gaaaatatca atcaagtgat
     6841 gaatatcctc aaaaaatatg gcggaaatga aatagagtca tattaaaaac ttccggaaac
     6901 ggaagttttt aatatgcttt accccaataa accattttat ctgcagactt tccacagcaa
     6961 acacaggtat ctgataaatg ctcttgttca aaaggaatac atcttgctgt agcccctgtt
     7021 tcttctttta ttttaatttc acaagcttca tcgccacacc acattgcttt tataaagcca
     7081 ggcgtatttg taataatatc tttaaattcg tccatatttt ttgcaatata agttttactg
     7141 tcccgcattt gacgtgcttt ttcaagtaaa tctttctgta aggtatccat taattcagga
     7201 attttgattt ctaattcatc catcgccact ttaatttttt ctcttgtatc tcttctaacc
     7261 aatatcactt gattattttc aatgtcttta ggtcctattt ctaagcggaa aggaaccccc
     7321 ttcatctcat actcactaaa tttccagcca ggcattttat cgctgtcgtc caattccact
     7381 ctgacaatag aacttaatct ttcttttatt tcatgagctt tttctaatac tccttcctta
     7441 tgctgtgcaa taggaataat aaccagctgg attggcgcaa ttctgggagg cagtaccaga
     7501 ccactgttgt ctccatgaac cataatcact gctccaatta agcgggtggt tacaccccag
     7561 gaagtttgat gaacatattg taattcattg tttttgtctg tgtatttaat atcaaaggct
     7621 cttgcaaaac catccccaaa gttatggctg gtacctgact gcaacgcttt accatcgtgc
     7681 attaagcttt ctatggtata agtttcctta gccccggcaa atttttcttt ttccgtcttt
     7741 ttacctttaa tcactggaat agctaaaacg ttttcacaaa attcagcata tacattcaac
     7801 atacggatag tttcttcctg agcttcctct gccgttgcat gggctgtatg accttcctgc
     7861 cataaaaatt ctaaagtacg taagaaaggt cttgtggtct tttcccaacg gaccactgaa
     7921 caccattgat tatataattt aggtaaatct ctgtaagatt gaataacatt agaatagtat
     7981 tcacaaaaga gcgtttctga agtaggtctt acacaaagcc tttctgttaa tgcttcattc
     8041 ccaccatgcg tcacccaggc cacttcaggt gcaaaacctt ctacatggtc cttttctttt
     8101 tgtaataaac tttctggaat aaaaagtggc atataaacgt ttttgtgacc agtttcttta
     8161 aaacgttggt ccaattgact ttgaatcaac tcccatatag catatccata tggctgaata
     8221 atcatacatc ctctgacact tgaatattca ataagctcag cctttttgac aacgtccgta
     8281 taccattgag caaaatcttc attcatgtct gtgatttcct gaacaaattt tttatctttc
     8341 tccattttaa ttccccttct cttcctttct ttttgtcctt catatacttt attcattata
     8401 acgttttttc taaaaaggta aaataatata aaaacctcca tcccaaaagg gaccgaggtt
     8461 ttcggcggta ccaccctaat aatgcatacg catcactcat ttttattaac gataaccaac
     8521 gttaccgctg tattcatcat acagaactcc aaggcgggtc ataaatacat tttttagaaa
     8581 tctcccacca acgattccct ctctgaaaaa aaatgtattt actattcctc ttcattgtct
     8641 ttgtctattt ttttgattaa taataaaata taacttactg tcttgtcaag gcatttaaaa
     8701 ttttttatgc tacttagccc gtatttgaca aatttcatcg gcattaatca gttctacttc
     8761 atctttatga gcaattattg tatcaatggt cttttgatta tctgccgttt taattacaat
     8821 aaaagctttt tcgcctactt caaaagcata catatattct acactgatac cattttttga
     8881 aagaatatct aatgcctttg ccaattcccc gggtctatgg ggtgttgcaa tacataatac
     8941 atctgttaat cttacggaaa aacccttttc ttttaatgct ttataggctt tatccggatc
     9001 tgtcagaatc attctgagaa tcccatattc tgccgtatca gcaatgctga gtgcagaaat
     9061 attaatatct aaatccccta aagtttttgt tacttccgtt aatcttcccg acttgttttc
     9121 caggaaaacc gataactgct taatgagcat tatatccctc cttaaagttt tctcctgtcg
     9181 ataacccttt tactttttcc ttctgttctt tcgatggtct ttggctctac aagtttaact
     9241 tttacagaaa ttcctagagt gctttctatc tgatgtttaa tttttgctga caaagattca
     9301 agatttctga tttcatccga gaagaagatt tcatccactt ctacccatac ttctaaagta
     9361 tccagattat ttatcctatc aacaataagt aaataatgag gcttagtttc tcccatattc
     9421 aacaatacac tttctatttg agatgggaat acgtttacgc cgcgaataat taacatatcg
     9481 tcacttcggc ctttacattt ttccatacga accaaagttc ttccacacgg gcaattatca
     9541 taatgaagaa cggttaaatc tcttgtcctg tatctgagaa gtggaagcgc ctcttttgta
     9601 atggttgtaa ataccagttc cccaacctct ccttctgaaa ccgtttctaa ggtatctgga
     9661 ttaatggtct caggaataaa atgatcttcg ttaatatgta ggccattttg catttcacat
     9721 tcacaagaaa caccaggacc aacaatttca cttaaaccaa atatatcaat cgcctttaaa
     9781 tgcaacttgc tttcaatctt tttcctcatt tcttcggtcc agggttcagc tccaaaaatc
     9841 ccaactctta aagaaagttc tttaggatca acgcccattt cttccatcgt ttcagccagg
     9901 taaagagcat aggagggtgt acatgctaac accgtacttt taaaatctct cataatttga
     9961 atttgctttt gtgtgtttcc gccggatata ggaatgacag ttgctccaac cttttcacta
    10021 ccataatgca aacctaagcc accagtaaac aaaccatatc cataagctat ctgaataaca
    10081 tcatttttcc tgacgccagc agcacaaaga cacctggcaa ccacttcaga ccaggttgtt
    10141 atatcttttc tggaataacc tactactgtc ggctttcccg ttgtaccaga agaggcatgc
    10201 agtctcatca cttcactcat aggtacagca aataagccaa aaggataatt atcccttaaa
    10261 tcctgctttg ttgtaaaggg aagtttattt aaatcttcaa tgcctcttat atctcctggt
    10321 tccatgccca tttcctgcat tttctttcta taaaaaggga cattatgata aaccctttca
    10381 accgtctcac atagtctttt actttggagt ttgatcattt cttcccggct catgcactca
    10441 ttatattcat tccaaatcat agtctaagtc tccccactct cttattaata atttcttatt
    10501 ttttatagta atcaaatatt ttacgctcat tggaacttta attttaagag ctgtcaggaa
    10561 gcagccagat aaagatattt cctgtttgga aaatacatta tttttaaatc attataaatg
    10621 aattatattt attatacaag ttattcactt tattcatttt ttatcgtgta tttatctcca
    10681 ctcaggaata attccaactt tctgcatggt ctttataaaa ataactacta taggacctgc
    10741 aataattcct ataacaccaa acatcttaat acctatgtac atagccatta aggtcacaag
    10801 gggataaacc cctatttgtg ttcctaaaac tttaggttct accatttgtc tgaataaaat
    10861 aaccacacca tatatgatta aaaaacctat cgctctgaaa taatcccccg taataaaatt
    10921 ataaatggcc cagggaataa gaatcccacc acttcccaaa atggggaaag catcaaaaat
    10981 accgataaca aatcctatta ataaagaata ttttaccctt agaattgtaa gacctataat
    11041 acatatactg gaaacaaata tcataagaat aagctgggtt tttatatatc ctaataaagc
    11101 cccaaataaa tcccttctgg cgatatctat ttttctgttc caactggctg gcaattgcct
    11161 ttcaataaag ttctggattt tcatcttatc tttactcata aagaaggttg caatcaaggt
    11221 cacaacactg acaaaaaaag cattcggaag ggccacaaca aagccaatag aacctttttt
    11281 aacacctggt ccgaccgcct ctgttaaact tttcatgatg gcatcaaaac catttcttat
    11341 taaatcctga agagaatccg gtaaggctaa aataaaggtt tctgtctttc ctcttaaact
    11401 ttctaaactc tccataaatc ctttataata agcaggaaaa tcctgtgcaa aagcctgagc
    11461 ttcctggata attctcataa cgattcctgt tactacagtt cctataaaaa caagaataat
    11521 aatgattgat aaaatacttg caagccctct gggcatcttg catttttttt gaaaccacct
    11581 gactaaaggc tccatggcag aagctaaaag ccaggcaata ataaagggtg caacatacga
    11641 aaaaatataa cggaaaaaca agtataagca taaaattaag aataaagctg tcagtaattt
    11701 attaatacta tctttgtgtt ttttataaaa ctctatcatt gtatcaccct tatacttgtc
    11761 ttatttttat tgatatttga ctttatgata aattatgata aatagcagaa aatattatta
    11821 tattattaat tatctattac ttgaattata ctacatgatt aaaagatatt aaacatttat
    11881 attagattat aaaattagat ttttaaaaaa attaaaggca ggttgccctg cctttttcat
    11941 tctaatggga tttctt
//
LOCUS       DAOSUD010000066        11278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3254_length_11278_cov_25.412367, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000066 DAOSUD010000000
VERSION     DAOSUD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_11278_cov_25.412367"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1411
                     /locus_tag="PLG49_08390"
     CDS             221..1411
                     /locus_tag="PLG49_08390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ17374.1"
                     /translation="MISEKMKVLVEGSSAIRAMFEEGKRLAKIHGEENVFDFSLGNPN
                     VDPPDRIKASIFEILNSEHPNFIHGYMNNSGYEDVRTKIANHLNQKHNLNLSFNSIVM
                     TCGAAGGLNIILKTLLNPGDEVIVFAPFFGEYRNYVSNYDGKLVVVDPDIETFEPNIE
                     ALKEKITSKTKVVIINSPNNPTGVVYSERAIKELTKTLSDKEKELGISIYLVSDEPYR
                     EIVYDGVEVPYVLKYYKNTFIGYSYSKSLSLPGERIGYIVANPEMDDFENIMSGLNVA
                     NRILGFVNAPSLFQRVIADCLDTEVDVNIYKRNRDLLYNHLVELGFTCVKPEGAFYLF
                     PRTPIEDDVAFCQEAKKFNLLLVPGSSFGCPGHMRLAYCISYEKIRNSLPAFEKLAKA
                     TIIK"
     gene            1604..2371
                     /locus_tag="PLG49_08395"
     CDS             1604..2371
                     /locus_tag="PLG49_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ17375.1"
                     /translation="MEEYTVKQLADMAGISSRTLRYYDEIGILKPARINASGYRVYGK
                     KEVDILQQILFYKELGVPLETIKEIIISPDFDSYTALIEHRQQLLKKRKQLDLLIANV
                     EKTIASLEGRIVMSDQDKFLGFKEKVIKENEEKYGKEIREKYGDKAVDESNAKWMNMS
                     QEQYEAFKKLEEELYQVLAAAMETNDPASDLAQKAADLHRQWLSFTWGSYSKEAHAGL
                     AEMYVADERFKAYYDKIKPGAAQFLRDAIRIYTQNNH"
     gene            2395..2862
                     /locus_tag="PLG49_08400"
     CDS             2395..2862
                     /locus_tag="PLG49_08400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOQ17376.1"
                     /translation="MKKLFYYHTPIGEIGIAEEDGSICAIFFSHEKISKEEYMIKESS
                     LIKNAYRQLVEYFNKQRKTFDLPLAPEGTEFQMKVWKALSDIPYGKTRSYKEIAIAIG
                     NPKAVRAVGMANNKNPIPIIIPCHRVIGSDGKLVGYGGGLEIKEYLLKLEGVL"
     gene            3024..4868
                     /locus_tag="PLG49_08405"
     CDS             3024..4868
                     /locus_tag="PLG49_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOQ17377.1"
                     /translation="MINKRRKAILSGRMISFILTAMILFVFTTACNNSRKIDGNEIVR
                     IGSNREIEEESTAEALNENHGESTKLTASNVKVNLALGNSWENAGVIYTQADITIHNN
                     GDFEISNWEVEIPVSENTKVDQIWNASYRVENGKIFFKAEEFNQTIPARGSVAFGCIL
                     AGIHEIDSNAIKVSYTENNTRVAANSSENHSNEQQTSREYIQPQKLIPKDVPPPTTDD
                     WLYVKGNKIVDKYGQEVWLTGVNWFGYNTGTNTFDGLWAADLNTSLAAIADRGFNILR
                     IPISSELILQWASGNPPKANFNEAINSYLVGMDSLQIFDYVVGQCRANGLKIMIDIHS
                     AKTDPMGHMKNVWFEGDITEQDYLDSLSWMAERYKDDDTIIAFDLKNEPHGKPHESPR
                     AIWNDSKDPENWKYVAEKAANAVLSKNPNVLVLVEGIEIYPKDIKSNGDYSSTNPEDY
                     YFNWWGGNLRGVKDYPVNLGKYQNKLVYSPHDYGPAVHAQPWFEGGFTYESLYRDCWR
                     DNWMYIYEDGIAPLLIGEWGGYMTEPNLSWMKCLRELIKKHKLHHTFWCFNANSGDTG
                     GLVLDDFVTWDEEKYTFVKEVLWTKDGKFVGLDHEIPLGKNGIALPKK"
     gene            4881..5432
                     /locus_tag="PLG49_08410"
     CDS             4881..5432
                     /locus_tag="PLG49_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17378.1"
                     /translation="MKKYKLALSYYLPSYLSMIVIIIFSFYFYIGTYKGAFNFVNFSI
                     FLVLLTGINIPLLINFIYIKSTCYVIDDEGITEKTLFRNKKILWDDINFMILTNNTTV
                     NRPGIDLLIKSKDNEEIAVHYQAYDFEEIYHTVLKGREELLKGPWTLSSIKPAMSDKK
                     AAFIMAVSGLLMLGLLIFTYYKA"
     gene            5630..7780
                     /locus_tag="PLG49_08415"
     CDS             5630..7780
                     /locus_tag="PLG49_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOQ17379.1"
                     /translation="MDFISGVVERITYTNEENGFTVIKIKSKGYSELITVVGNFASVN
                     VGASLQIKGQWKYDSKYGKQFQALDFKETVPATIVGIEKYLGSGLIKGIGPVYAKRIV
                     KHFKEDTLRIIEEEPDRLIEVEGIGEKRLQMIKKDWQEQKEIKNIMIFLQSYGVSTSY
                     GVKIYKTYGDESIQIVKENPYRLADDIWGIGFKTADKIAEKLGYRKDSPERCRSGIIY
                     LLNDLSNEGHCYVMKEELTEKAVHILEVSHELVERTIAVMLEEKSLIIEESGAIYLPP
                     FYHSETGVVKRIKEILACDIPKSEITIEKALESIQKEHHIQYDEIQLKAVKCAANSKF
                     MVLTGGPGTGKTTTTLAIIEIFKRMNKKILLAAPTGRAARRMSEATGMEAKTIHRLLE
                     YKPSEGYQKNESNPLDCDVLIIDEVSMVDIILMYNLLKAVKNSTIVILVGDVDQLPSV
                     GAGNLLKDIIDSAAIPVIKLTKIFRQAQGSAIIINAHRINKGLMPILKGGKNTDFFFI
                     PEEEPSKVAEIIKELCSKRLPRYYQVDPIEDIQVLCPMQRGECGAYNMNILLQEALNP
                     SKTTIRYGGTNYRLNDKVMQIKNNYDKNVFNGDIGRIVKINQEDKILTIRFDDNDIDY
                     DITELDEVVLAYATTVHKSQGSEYKIVVAPFTMQHYMMLQRNLLYTCVTRAKKVLVLV
                     GSKKAISIAVANNKIQKRNTLLESRLKESLCINF"
     gene            complement(7884..8402)
                     /locus_tag="PLG49_08420"
     CDS             complement(7884..8402)
                     /locus_tag="PLG49_08420"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOQ17380.1"
                     /translation="MKAKTPKESVVEMTELVLPNDTNLLGNLLGGKMMHWIDIAGAMA
                     ASRHSNTAVVTACVDSLDFRYPVRVGELVMLNAKLTWVGRTSMEVKVKVYTENIKTGE
                     TILTNKAYITYVALDDEGKPTPVPPLCPETDEEKKDYQEAEMRRNERLKKKAQKLKNV
                     SIENNYSPYTDQ"
     gene            complement(8498..9379)
                     /locus_tag="PLG49_08425"
     CDS             complement(8498..9379)
                     /locus_tag="PLG49_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ17381.1"
                     /translation="MKKVKKIQLVLWLVLFANLTVAFIKIVIGYLIKSAGVMTDGLHS
                     ITDMSSNIIGLIGIHFAYKPGDKKHPYGHRKIENLAGLLIALLLLILAIKQSADAIKR
                     FFHPLPLEISLLSLLLLILSLIINIFIAYYEYKQGKALNSTILISDSIHTKSDIFISL
                     GVLTTLIAVKLGLPAFIDSVASLIVSVFILAAAYEIFKRTSGPLLDQAILDEDKIKEI
                     VLSFHEVKDVHDIKSRGTEDDVYLDFHIIIDPSISFMESHQLTDSIEKKLKSNFCENM
                     NINIRLEPCPLWENDHS"
     gene            9624..10262
                     /locus_tag="PLG49_08430"
     CDS             9624..10262
                     /locus_tag="PLG49_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17382.1"
                     /translation="MRFRFLLLAFMIVIAFNLSGCENNQNGSKESMSQGIDQPIEEMA
                     TDHEPEDKDEAEDINENLKLPKEDESVTVIYGQKDNAWTYITDEGTKLREYAIDLDGD
                     GAEEKIQLYTAAQRDENGDILWDDSQDWLLLVEDQNAYYPLFAEPVQIGMVYFNVFYD
                     ENDLPKISLLLSAGASMRITNYYYDDALNGYKAENVYEENSINSMYSTIPYY"
     gene            10310..11263
                     /gene="menA"
                     /locus_tag="PLG49_08435"
     CDS             10310..11263
                     /gene="menA"
                     /locus_tag="PLG49_08435"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOQ17383.1"
                     /translation="MKLKSFLKLVEIQTKLASVIPFLLGTVYAIYRFHEFNIINFILM
                     FISLIHVDMMTTAINNYIDYKKAKKNHGYNYELHNAIVRDNLKESTVLVTIFILFIIA
                     VVSGLILYLRTDIIVLLLGAISFLVGICYSFGPVPISRIPLGEVFSGFFMGFVITFIA
                     DYIHVFDKGLIRIAYNNSILSLFVNLKEIVYIFLLSLPAVIGIANIMLANNICDIEDD
                     IENKRYTLPIYIGKEKSLMLYKILYYFLYLNILLTILIGINSFVVVLTLITFIVVSRN
                     IKIFYDKQTKKDTFSTAVQNFALVNIIYILSICIGILFPLG"
BASE COUNT         3931 a         1593 c         2191 g         3563 t
ORIGIN      
        1 atgaatgcct taataaatag tttaatatat ttttacaaac tagtcaaaaa atttatgatt
       61 tttacttgtt agattcaaaa attatttttg aaatttgatg cattaaaaga caaagaatca
      121 taaaattttg agctgggttg attgatttta atctattaaa atagtaaaat ttaactatgc
      181 attttaaatc atgaattgcc aataaaagga gagcgtaaaa atgatatcag aaaaaatgaa
      241 agtattagtt gaaggcagct ctgccatcag agcaatgttt gaagaaggta aaagacttgc
      301 caaaattcat ggtgaagaaa atgtatttga tttcagtttg gggaatccca acgttgaccc
      361 ccctgaccgt attaaagcgt ccatattcga gatattaaat tcagaacatc ctaactttat
      421 ccatggatac atgaacaatt ccggctatga agatgtcagg actaaaattg ccaatcatct
      481 taatcaaaag cacaatctga atttgtcttt taatagtatt gttatgacct gtggggctgc
      541 agggggttta aatataattt taaagacttt gcttaatccg ggagatgaag ttattgtatt
      601 tgcgcctttt tttggagaat accgtaatta tgtcagcaat tacgacggga aacttgtagt
      661 ggtagatccg gacattgaaa cttttgagcc taatatcgaa gccttaaaag aaaaaataac
      721 atccaaaaca aaagttgtaa ttattaatag tcccaataat ccaacagggg ttgtttattc
      781 tgaaagagcc attaaagaat tgacaaaaac cctttcggac aaagaaaaag aattaggaat
      841 cagtatttat cttgtatctg atgaacctta cagagaaatt gtatatgatg gggtagaagt
      901 accttatgta ttaaaatatt ataaaaatac atttataggt tattcttata gtaaatcttt
      961 atctcttcca ggagagagaa taggctatat tgtagccaat cctgaaatgg atgattttga
     1021 aaatatcatg tctggcttaa atgttgctaa taggattctt ggctttgtaa atgcaccttc
     1081 tttatttcaa agagtgattg ctgattgttt agatactgag gtagatgtaa atatctataa
     1141 gagaaacaga gacttgttat ataatcattt ggttgagctg ggctttactt gcgttaagcc
     1201 agaaggggcc ttctatttat tccccagaac gcctatagaa gatgatgttg ctttttgcca
     1261 ggaggctaaa aagtttaatc ttttactggt acctggttca agttttggat gtccgggtca
     1321 tatgagattg gcctattgca tttcttatga aaaaattaga aattccttac ctgcttttga
     1381 aaagttagca aaagcaacaa taataaaata aaaagtaaaa aagccttttg ttagtatact
     1441 aacgaaaggc ttttttgtct tggaatgtct taattatatt ttttcagaaa atggagaaaa
     1501 acatccttga cattaacgca gcgtaagggt atatgatgaa cacagatgcc tatattaaag
     1561 caggaaggaa aataatattt aagtttttta aggagttgct tatatggaag aatatacagt
     1621 taaacaattg gcagatatgg caggcataag cagccggaca cttcgttatt atgatgaaat
     1681 cggtatatta aaacctgcaa gaatcaatgc gtctggttat cgggtttatg gaaaaaaaga
     1741 agtagatatc ttgcagcaaa ttttatttta caaagaatta ggcgttccct tagaaaccat
     1801 aaaagaaatt atcatatctc ctgattttga ttcctatact gctttgatag agcatcgcca
     1861 acagcttctt aaaaaaagaa agcaattgga tttactgata gcaaatgtag aaaagaccat
     1921 agcgtcatta gaagggagaa ttgttatgtc agatcaagat aagttcttag gttttaaaga
     1981 aaaagttatt aaggaaaatg aggaaaagta tgggaaagaa atacgggaaa aatacggaga
     2041 taaagcagtg gatgaatcta atgctaaatg gatgaatatg agccaggaac agtatgaagc
     2101 atttaaaaaa ttagaagaag aattatatca agtattagcg gctgccatgg aaacgaatga
     2161 tccggcaagt gacttagccc aaaaagcagc agatctgcat cggcaatggt tatcttttac
     2221 ctggggcagt tacagcaaag aagcccatgc tggtttagcc gaaatgtatg tggcagatga
     2281 gcgttttaaa gcctattatg ataaaataaa accaggggca gcccaatttt tgagggatgc
     2341 gataagaatt tatactcaaa ataatcacta acagtttttg gtttggagga gtatatgaaa
     2401 aagctatttt actaccatac acctattggt gaaattggca tagctgaaga agatggcagt
     2461 atttgcgcta tattttttag tcatgaaaag atttctaaag aggaatatat gattaaagaa
     2521 agcagcctta taaaaaatgc ctacaggcaa ttagttgaat attttaataa acagcgaaaa
     2581 acatttgatt tacctctagc tcctgagggt acggaatttc aaatgaaagt ctggaaagcc
     2641 ctgagtgaca ttccttatgg taagacccgt tcttataaag agattgctat tgcgattgga
     2701 aatcctaagg ctgtcagggc tgtgggaatg gcaaataaca aaaatcctat acccataatc
     2761 attccttgtc acagagtcat tggttctgat ggtaaactgg tgggttatgg aggcggttta
     2821 gagataaaag aatatttatt aaagctggaa ggcgttttat agaactttga ccattcttga
     2881 gtattaaaag ctatggagga atccatagct tattttttat tacttagttt gatttatatg
     2941 gtataatata aaataatgtg aaattataaa ataatgtact tatttttaag gttataaata
     3001 ttgttgttaa tggggtggaa aaagtgatta acaaaagaag aaaagccatt ttatctggaa
     3061 gaatgatttc tttcatttta actgcaatga ttttatttgt atttactact gcttgtaata
     3121 acagcaggaa aatagatggc aatgaaattg taagaattgg cagtaataga gaaatagaag
     3181 aagaaagcac agcggaagct ctgaatgaaa atcatgggga aagtactaaa ctgacagcat
     3241 ctaatgttaa agtgaatctt gctttaggca acagttggga aaatgctggt gtcatttata
     3301 cgcaagcaga tattaccatt cataataatg gggattttga aatctctaat tgggaagtag
     3361 aaattcctgt atcagagaat acaaaagttg atcaaatctg gaatgcttct tacagagttg
     3421 aaaatggcaa gatatttttt aaagcagaag aatttaatca aacaattcct gccagaggca
     3481 gtgtggcttt tggctgcatt ttagctggta tccatgaaat tgacagcaat gccattaaag
     3541 tatcctatac ggaaaacaat acaagagtgg ctgcaaacag cagtgagaat cactcgaatg
     3601 agcagcagac ttcaagggaa tatattcagc cacaaaagct gataccaaag gatgttccgc
     3661 ccccaacgac agatgactgg ctttatgtaa aaggcaataa aattgtagat aaatatggac
     3721 aggaagtttg gttaacgggt gtcaattggt ttggctataa cacaggaacc aatacttttg
     3781 atggtctttg ggcagctgat ttaaatacat ctttggctgc catagccgac agaggtttta
     3841 atatattaag aataccgatt tccagtgagt tgattttaca gtgggcaagt ggaaatccgc
     3901 ctaaagccaa ttttaacgaa gccattaatt cttacctggt aggcatggat agcttgcaaa
     3961 tctttgatta tgtagtagga cagtgcaggg caaatggact aaaaataatg attgatatcc
     4021 attcagccaa aacggatcct atgggacata tgaagaatgt ttggtttgag ggggatatta
     4081 cggaacagga ttatttagat tctctttcct ggatggctga aagatataaa gacgatgata
     4141 ccattatagc ctttgactta aaaaatgaac ctcatggaaa accgcatgaa tctccaaggg
     4201 caatctggaa tgattcaaaa gaccctgaaa attggaagta tgttgcagaa aaagctgcca
     4261 atgcagttct ttcaaaaaac ccgaatgttc ttgtattagt cgaaggaata gaaatatatc
     4321 caaaggatat taaaagcaat ggggactatt catctaccaa tcctgaagat tactacttta
     4381 attggtgggg cggcaattta agaggggtaa aggattatcc tgttaatttg ggcaaatatc
     4441 aaaacaaact ggtctattct cctcatgatt atggtcctgc tgtacatgcc cagccctggt
     4501 ttgaaggagg ttttacttat gaaagtctat acagggattg ctggcgggat aactggatgt
     4561 atatatatga agatggtata gcgcctctat tgattggaga atggggaggt tatatgactg
     4621 aacctaacct gagttggatg aaatgtttaa gagagttgat taagaaacat aaattacatc
     4681 ataccttctg gtgctttaat gccaattcag gggatacagg tggtttggtt ctggatgatt
     4741 ttgtaacctg ggacgaagaa aaatatacct ttgtcaaaga agtattatgg acaaaagacg
     4801 gcaagtttgt cggcttagat catgaaattc cattgggcaa aaatggaatt gctttaccaa
     4861 aaaaataaaa gggggatgct atgaaaaaat ataaattggc tttatcttat tatcttccgt
     4921 catatttatc gatgatcgtc ataatcattt tttctttcta cttctatatt ggaacttata
     4981 agggtgcatt taattttgtt aatttttcaa tctttttagt cttattaaca gggattaata
     5041 ttcctttgtt aattaacttt atttacatta aatcgacttg ttatgtaatt gatgatgaag
     5101 ggataacaga aaaaacactt tttagaaata aaaagatttt atgggatgat attaacttta
     5161 tgattttgac caacaataca acggtaaaca gaccaggcat tgatttgtta atcaaaagca
     5221 aagacaatga agagattgct gttcactatc aagcttatga ttttgaagaa atttatcata
     5281 ccgtattaaa aggcagggaa gagcttctta aaggaccctg gaccttatca tccattaaac
     5341 ctgctatgtc cgataaaaag gctgccttta ttatggctgt atcaggctta ctgatgttag
     5401 gcttattaat atttacttat tataaagcat aagtttacaa catatagtgg gtaaaggaac
     5461 tggatggtgt gttatcagaa gaggataaaa gaggaaaaat agtggaatag ttgtagggct
     5521 tagagcttta caacttttat ttttaagtgc tttaatgatc ttatgatata ataaatttta
     5581 taatgggtaa atatttaaat gaatataaaa aatataggat ggttttatga tggattttat
     5641 tagcggagtg gttgagagaa ttacatacac gaatgaagaa aatggcttta cagtgattaa
     5701 aattaagtca aagggttatt cagagctaat aactgttgta ggtaattttg cttcggttaa
     5761 tgtaggagca agtcttcaaa taaaaggaca gtggaaatac gacagtaaat acggcaagca
     5821 atttcaagcc cttgatttta aagaaaccgt tcctgctacc attgtcggga tagaaaaata
     5881 tttaggaagc ggcttaatta aagggatagg acctgtgtat gccaaaagaa tcgtaaaaca
     5941 ttttaaagaa gataccctta gaattattga agaagagcca gaccgtttaa tagaggtaga
     6001 aggaataggg gaaaaacgtc tgcagatgat caaaaaggac tggcaggagc aaaaggaaat
     6061 aaaaaatatc atgatctttt tacaaagtta tggggtttct acaagctatg gggtaaaaat
     6121 ttataagact tatggagatg aaagtattca aattgtaaaa gaaaatccct atcggctggc
     6181 cgatgatatt tggggaattg gttttaaaac tgctgataaa attgctgaaa aattgggcta
     6241 tagaaaagat tctcctgaac gttgccgttc aggtatcata tatcttttaa atgacttgtc
     6301 caatgaaggg cattgttatg tgatgaaaga ggaattaaca gaaaaagccg tccatatcct
     6361 tgaagtaagt catgaattag tggaaagaac cattgccgtg atgttggaag aaaaaagtct
     6421 gattatagaa gaaagcggag ccatttactt gccgcccttt tatcacagcg aaacaggtgt
     6481 tgtaaaaaga ataaaagaaa tccttgcctg tgatattcct aaatcagaaa taacaataga
     6541 aaaagccctg gaaagcattc aaaaagaaca tcatattcaa tatgatgaga tccaattaaa
     6601 ggctgtaaaa tgtgcagcca attcaaaatt tatggttctt actggtggcc cggggacagg
     6661 taaaaccaca accactctgg ccatcatcga aatctttaaa aggatgaata aaaagatact
     6721 tttagcggca ccgacaggaa gagcagccag gagaatgtca gaagcaacag ggatggaggc
     6781 aaaaaccatt catcgcttac tggaatacaa gccgtcagaa ggttaccaaa aaaatgaaag
     6841 caatccttta gattgtgatg tattaatcat agatgaagta tctatggtgg atatcatttt
     6901 aatgtataat cttttaaaag cagtaaaaaa cagtacgatt gtcatactcg tgggggatgt
     6961 tgatcaattg ccgtcggttg gggcaggaaa cttattaaag gatattattg attcagcggc
     7021 tattccggtt ataaaactga cgaaaatttt tagacaagct caaggaagtg ctatcattat
     7081 caatgctcac aggattaata aaggcctgat gcctattcta aagggaggaa agaatacaga
     7141 ttttttcttt atcccggaag aagagccttc taaagtggca gaaatcataa aagagctttg
     7201 cagcaaaagg ctgcctcgct actatcaagt agatccgata gaggatattc aggtattatg
     7261 ccctatgcaa agaggagaat gcggagcata taacatgaac atccttttac aggaagcctt
     7321 gaatccttca aaaacaacta tccgttatgg gggtacaaat taccgcttaa atgataaagt
     7381 gatgcagata aagaataatt acgataaaaa tgtatttaac ggggacatag gccgtattgt
     7441 aaaaatcaat caggaagata aaattctgac tatccggttt gatgacaatg acattgatta
     7501 tgatattact gaattggatg aggtcgtttt agcctatgca acaacagttc ataaaagcca
     7561 gggaagtgaa tataaaattg ttgttgcccc ttttactatg caacattaca tgatgctgca
     7621 aagaaatctt ttatatactt gtgtcaccag agccaaaaaa gtccttgttt tagtaggttc
     7681 aaaaaaagcc atttccatag cggttgccaa taataaaatt caaaaaagga ataccttgct
     7741 cgaatcaaga cttaaagaaa gtttatgtat taatttttaa tataaataag aggctgcagc
     7801 taaacacttt tcgcctgtac agccgccttt tttaacaatc aaaaaaaact atacagcaat
     7861 actgctgcac agctttttta gtcttattga tccgtataag gactataatt attttctata
     7921 gatacatttt ttaatttttg ggctttcttt tttaatcttt catttcttct catttctgct
     7981 tcctgatagt cctttttttc ttcatctgtt tcaggacaaa ggggtggaac aggagttggt
     8041 tttccttcat catccagggc tacataagtg atgtaagctt tgttggttaa aatcgtttcc
     8101 cctgttttaa tattttcggt atatactttt acttttacct ccatggaagt tctgccaacc
     8161 caggttaatt ttgcattaag cataaccagt tcacctaccc ggacggggta tctgaaatcc
     8221 agactgtcaa cacaagctgt cacaacagct gtattagaat ggcgggatgc agccatagca
     8281 cctgcaatat caatccagtg catcatctta ccgcccaata aattgccaag gagatttgta
     8341 tcattgggaa gaaccaattc tgtcatttca acaacggatt cttttggtgt tttagctttc
     8401 ataaattgcc tcctttactt atcttttgct gaggtttcca gagcttattt ggaagtatta
     8461 taccataatt agtatcaata taaaaataat taatattcta tgagtggtca ttctcccaga
     8521 ggggacaggg ttccaggcga atattgatat tcatattttc acaaaaatta ctttttagct
     8581 tcttttcaat ggaatctgtc agttgatgag attccataaa acttatgctg ggatcaataa
     8641 taatatgaaa atctaagtaa acatcatctt cagtcccacg actttttata tcatgcacat
     8701 ctttaacttc atgaaaacta aggacaattt ctttaatttt atcctcatca agaattgcct
     8761 gatctaataa gggtcctgag gttcgtttaa aaatttcata ggctgctgca aggatgaata
     8821 cagatacaat caatgaagcg acagaatcaa taaaagcagg taacccaagt ttaacagcaa
     8881 ttaaagttgt caatactcct agagaaataa aaatatcact ttttgtgtga atggaatcag
     8941 aaattaaaat agtactgttt aaggctttgc cttgtttata ttcatagtaa gcaataaaaa
     9001 tattaataat aagacttaaa ataagcaata aaagacttag aagactgatt tctaggggaa
     9061 gaggatgaaa gaatctttta atggcgtcag ctgactgctt tatggcaaga atcaaaagta
     9121 agagagcaat caaaaggcct gcaagatttt caatcttcct gtgaccatat ggatgttttt
     9181 tatcgccagg tttatatgca aaatgaatac ctattaagcc gataatattg gaactcatgt
     9241 ccgttataga gtgaagaccg tcagtcatta cacctgcgct ttttataaga tagccaataa
     9301 cgattttaat gaaagcgaca gttaaattag caaacaaaac aagccacagg actaactgaa
     9361 tctttttaac ttttttcata atattgcctc caaaaaataa tcaaaaaatt ttatagatag
     9421 gactaggttt tcaattgaga taatatatga ttattatatt cctgaaaaaa tgttttgtaa
     9481 atagttgttg gaagattata tgaattatca aatgataata agattaattg tcattaatat
     9541 aataaatatc aaattaatat taagtgtgct tttatggtat aatagacgtg tttaatctta
     9601 atactttaaa agaaggtgat ggaatgagat ttcgtttttt attgttagca tttatgattg
     9661 tgattgcctt taatttatca ggttgtgaaa ataatcaaaa cggttcaaaa gaatccatga
     9721 gtcaggggat tgatcagcct attgaagaaa tggcaacgga tcatgagcct gaagataaag
     9781 atgaggctga agatataaat gagaatttga aattaccaaa agaagatgag tctgttacag
     9841 tgatttatgg acaaaaagac aatgcctgga cttatataac cgatgaggga acaaagctta
     9901 gggaatatgc tatagactta gatggggatg gtgcagaaga aaagattcaa ttatatacgg
     9961 cagcacaaag agatgaaaac ggggatatac tctgggatga tagtcaggac tggcttttat
    10021 tagtagaaga tcagaatgcg tattatccgt tatttgcaga accggtgcaa atcggaatgg
    10081 tatattttaa tgttttttat gatgaaaatg atcttccaaa gattagcctt ttactatccg
    10141 ccggtgcgag tatgaggatt acaaattatt attatgatga cgcactcaat ggatacaaag
    10201 cagaaaatgt ctatgaggaa aacagtatca attctatgta cagcaccata ccttactatt
    10261 aaatcaaaat gaaataagtt gctggcgtat taataaaagg gtggtaagga tgaagttaaa
    10321 aagttttttg aagttggtag aaatacaaac aaaattagca agcgttattc cttttttgct
    10381 gggaactgtt tatgccatat atagatttca tgaatttaat atcataaatt ttatcttaat
    10441 gtttatttct ttaattcatg tggatatgat gacaacagcg attaataact atatcgatta
    10501 taaaaaagca aaaaaaaacc atggttataa ttatgaatta cacaatgcca ttgtaagaga
    10561 taacttaaaa gaatccacgg ttttagtcac tatttttatc ttgtttatta ttgctgttgt
    10621 atcgggtctt attttatatt taagaacgga tattattgtt cttttgcttg gagctatatc
    10681 ctttttggtt ggtatttgtt attcttttgg tccggttcct atttccagga tacctttggg
    10741 agaagtattt tcaggatttt ttatgggctt tgttattact tttattgcag attatattca
    10801 tgtttttgac aaaggactta ttcgtatcgc gtataataat tccattcttt ctctttttgt
    10861 taatttgaaa gaaattgttt atatttttct tctttcctta cctgccgtta taggtatagc
    10921 taatattatg ctggccaata atatttgtga tattgaagat gatattgaaa acaaacgata
    10981 taccctgcca atttatattg gcaaagaaaa aagtttaatg ctttataaaa tactttatta
    11041 ctttttatac ttaaatattc tgctgacaat tcttattggt attaattcgt ttgttgttgt
    11101 actgacgctt attacattta ttgttgtttc taggaatatt aagatttttt atgataagca
    11161 aacaaaaaaa gatacttttt ctacagctgt tcaaaatttt gcattagtaa atattattta
    11221 tattttaagt atttgtatag gtattttatt tcctttgggt tagtagtctt ggtaagta
//
LOCUS       DAOSUD010000067        10582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3499_length_10582_cov_21.495488, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000067 DAOSUD010000000
VERSION     DAOSUD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_10582_cov_21.495488"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..391
                     /locus_tag="PLG49_08440"
     CDS             74..391
                     /locus_tag="PLG49_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HOQ17384.1"
                     /translation="MNQHIITMIYNYFIALGVILGGSLFGSMAAVFCKEKPVKMMLVF
                     SEQLKLWAIIVALGGTFSSFEVLELGILKGEIKSVIKQILYILSAFAGAHTGYIILNH
                     LGG"
     gene            398..850
                     /locus_tag="PLG49_08445"
     CDS             398..850
                     /locus_tag="PLG49_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17385.1"
                     /translation="MKKTSSILWIFILGFIGGMMSNHMLYMHELEKSLNENEILSTKL
                     ADSLIKLNQLENNDFKKEKLILKDIVPVNTNKDEPVIPKNEIDQYIKLLLNNQIGKEV
                     KNIDIHLIYNALNNRILKYQDGNYQLKIRAILVSEMMHIYYSVEKIQI"
     gene            958..1182
                     /locus_tag="PLG49_08450"
     CDS             958..1182
                     /locus_tag="PLG49_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOQ17386.1"
                     /translation="MKTRPLDFLSLLLIIIGALNWGLIGFFQFDLVASLFGGTDSWLS
                     RIVYALVGLAGLYGLSLFGRIDEERLGERE"
     gene            1311..1724
                     /locus_tag="PLG49_08455"
     CDS             1311..1724
                     /locus_tag="PLG49_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOQ17387.1"
                     /translation="MIQIISGEKGEGKTKHLIDMANTKVKETPGHIVYLNNDREHMYQ
                     LHYKIRFIVVDDFPVKNSKEFLGFICGIIAQDHDIDHIYIDGLLKMTNLTLEEAYNLI
                     EELRQVGEKFNVNFVIGMSCRNKDIPESLKSYLIA"
     gene            complement(1783..2943)
                     /locus_tag="PLG49_08460"
     CDS             complement(1783..2943)
                     /locus_tag="PLG49_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ17388.1"
                     /translation="MKKKRIISFKYKLSIIISFWIFLAFNLIAFNIYLNTNDSLEKQY
                     NLRKYADTNVSLSNYNFLYSNYNNSDFYLLIMSVLFLVISIVLGALIVFYISNPIKKM
                     MIGIEEVSNGNYDYMIEINTTDEFNMLGKSINNLSRKMKVLIEEKCNKERELLLKDSQ
                     IEMQKDVIASQSEQISMLNEKLEELFSENKNICIAAINALTNSLEVKDVYTRGHCERV
                     REYVVKIGQALNLSKNEINELEFAALLHDIGKIGIPSRILNKEDELTEEEAELIKMHP
                     RLGYEIVKNLPFLDNAKYAILQHHERIDGKGYPDGLVDEEISLYSKILAVADAFDAMT
                     TGRKYRIIPYPKEVALEQLIHNKNIQFDVRIVDVFVDIIKKEMNEDKNKIPS"
     gene            3102..4316
                     /gene="pepT"
                     /locus_tag="PLG49_08465"
     CDS             3102..4316
                     /gene="pepT"
                     /locus_tag="PLG49_08465"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOQ17389.1"
                     /translation="MTNVAERFLKYVKFPTSSNESSPTCPSTKNQLEFAKYLISECQT
                     IGLEEVELDENGYVMATLPSNTDKDVETLGFIAHMDTSPDMSGENIQPKMIKNYNGED
                     IVLNEEKNIILSPKVFPNLKKYTGHTLITTDGNTLLGADDKAGIAEILTAMEYLIAHP
                     EIKHGKIRIAFTPDEEIGRGADLFDVQKFAADFAYTIDGGLLGELQYENFNAAYCKIT
                     INGQNVHPGTAKEKMKNAVRIGTELANLFPNEEIPEKTDGYEGFFHLNSFNGNVEKAE
                     LTYLIRDFDMTHFKQRKEFIEDTVRMMNERYGQGTIHLDLRDQYYNMKERLESKMDIV
                     NRAFNAMKAVGIEPVVRPIRGGTDGARLSFMGLPCPNLFTGGDNFHGKYEYISVEAME
                     KAVEVIVKIAEI"
     misc_binding    4381..4583
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4666..5892
                     /gene="tyrS"
                     /locus_tag="PLG49_08470"
     CDS             4666..5892
                     /gene="tyrS"
                     /locus_tag="PLG49_08470"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOQ17390.1"
                     /translation="MNNVFDVLQERGFIQQTTHEKEIRELLEKEKVTFYIGFDPTADS
                     LHVGHFVTIMAMAHMQKAGHRPIALIGGGTAMIGDPTGKTDMRKMMTEETIQHNAECF
                     KKQLSRFIDFSNDKAIMVNNADWLLKLNYISFLREIGVHFSVNKMLTYESYKTRMEKG
                     LSFLEFNYTLMQSYDFLELYRRYNCKLQLGGNDQWSNILGGVELIRRIGHDDAFGMTF
                     TLLTTSEGKKMGKTESGAIWLDPQKTSPYDFYQYWRNVADADVKKCLSLLTFLPMEEV
                     NRLAALEGAEINKAKEILAYEVTKIVHGEEEAIKADTAAKALFKGGAEGGSIPTTEIT
                     KEELVKGMDILTLLQKTNLVPSRSEGRRLIQQGGIKVQNQKISDIDYLITEKDFENSS
                     LMIQKGKKVFHRVQLI"
     gene            complement(5958..7178)
                     /locus_tag="PLG49_08475"
     CDS             complement(5958..7178)
                     /locus_tag="PLG49_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ17391.1"
                     /translation="MKLPWDKHYLKISFHVIFTLIVIFGLAIFIYNIIPITATIAAMI
                     SRILSILSPFFIGVAIAYLLDPMVDIYQRRVVEPLGKFLKDKNIIFGKKDNNEKSQDK
                     EKEISRFLPTLLTYLTLILALTIIGILLSKSLGNHKGFKNLDNLVSSIKSYVQSFNNM
                     ITNLQQGLEDIGFLQKAEETLTEFSGRLGNYVQNIALFFIEKITKAGNRVVDLVLAFV
                     IAFYLLKDKAKLLRVFNQFFRILLPEKIYIRLKYLCIDMDTVLSGYIRGQLIDSLIMG
                     VLIGISVAIIGIDFPIIIGIIAGIANVIPYFGPIIGMIPAAFMGLISDNPMKALYACI
                     TLFILQQIDGAIIAPKVVGESVDVHPVAIVVSILIGGSLFGLLGMLIAVPIAAFLKLL
                     LIRFMERKSEEKTP"
     gene            complement(7276..8733)
                     /locus_tag="PLG49_08480"
     CDS             complement(7276..8733)
                     /locus_tag="PLG49_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOQ17392.1"
                     /translation="MASTVKELEPKMVWEYFESISQIPRGSGNEKAISDYLVEFARQH
                     QLEVIQDKALNVIIKKKASPGYENAPVVILQGHMDMVCEKNKDTVHDFLKDPLDLVVE
                     GDYIFARGTSLGGDDGIALAYGLALLASDNIPHPPLEVVFTTSEETGMEGAANLDCSH
                     LKGKILINLDSEEEGVFLSSCAGGARAHLILKTQWVKEEKSGPVYSIQIRGLKGGHSG
                     QEIDKERANANLLMGRILYSLQEDMHFDLISINGGAKDNAIPREADAVIQIESKNIEK
                     LMKIIEKWNDILKNEYKKSDPDIEVKIELVREISSEHLSEEIKGKIIHILMLMPNGVQ
                     SMSMDIEGLVESSTNLGVVITDKEEIRFISALRSSVESRKRLIINKIKVLASFVGADF
                     LLKGEYPAWQYAEDSKIRSFFTNKYEELFGKKAEITAIHAGLECGFFAEKIKDVDIIS
                     LGPNMKDIHTPNERLSISSTKNTWELLLAVLKEIK"
     gene            complement(8818..10176)
                     /locus_tag="PLG49_08485"
     CDS             complement(8818..10176)
                     /locus_tag="PLG49_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOQ17393.1"
                     /translation="MITRFEVQETNQMIEQAKLDVRTITLGINLLDCADSNMDTLNRK
                     IYDKITKTAEHLVKVGEDIEKEFGIPIVNKRISVTPISIVAAATNAQSYVPVAHTLDR
                     AAKAVGVNFIGGFSALVHKGCNASDNVLIQSIPQALAETERVCSSVNLGSSKTGINMD
                     AVKEMGKIIKDTAVKTKDKDSIGCAKLVVFCNAPEDNPFMAGAFHGVGEKDAVINVGV
                     SGPGVVKKALEEVKNKDFETLCETVKRTAFKITRVGQLVAREASQRLNIPFGIIDLSL
                     APTPAIGDSIAEILEEMGLESVGAPGTTAALALLNDNVKKGGIMASSYVGGLSGAFIP
                     VSEDQGMIKAVEKGSINLEKLEAMTCVCSVGLDMIAIPGDTSEETISGIIADEMAIGM
                     VNGKTTAVRIIPVIGKGVGDVVEFGGLLGYAPIMAVNSFSCKDFINRGGRIPAPVHSF
                     KN"
     gene            complement(10199..10468)
                     /locus_tag="PLG49_08490"
     CDS             complement(10199..10468)
                     /locus_tag="PLG49_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ17394.1"
                     /translation="MKGIITVVGKDKVGIIAKVCTLLSEKNINILDISQTIVQGYFNM
                     MMIVDLTNATDDFGKIEDELIELGKQIGVVIKLQREEIFNSMHRI"
BASE COUNT         3546 a         1885 c         1634 g         3517 t
ORIGIN      
        1 ctaattttat tttatttctc ttttttaaaa catagaatta aatagcacgc ttttatcata
       61 acagggggta aaaatgaatc aacatattat aacaatgatt tataactatt ttattgcttt
      121 aggggttatt ttaggcggat ccttatttgg ctctatggct gcagtgtttt gcaaagagaa
      181 gcctgtaaaa atgatgctgg ttttttcaga acaattaaaa ctatgggcaa ttattgttgc
      241 tttaggcgga accttttctt cttttgaagt tttagaactg ggaattttaa aaggtgaaat
      301 aaaaagtgtt atcaaacaaa ttttatatat attaagtgct tttgcaggag cccatacagg
      361 atatattatt cttaaccatt taggaggtta gcaaaatatg aaaaagacct cttcgattct
      421 gtggattttt atattgggct ttataggcgg aatgatgtcc aatcatatgc tttatatgca
      481 tgaattagaa aaatctctta atgaaaatga aattttatcc acaaaattgg cagacagttt
      541 aataaaacta aatcaattag aaaataatga ttttaaaaaa gaaaaattaa tcttaaagga
      601 tatcgtgcca gtcaatacga ataaagacga gcctgttatt cctaaaaacg aaattgatca
      661 gtatataaaa ctattactga ataaccagat tggtaaagaa gtaaaaaata tcgatatcca
      721 tttaatatat aatgccttaa ataaccgtat attaaaatat caggatggta actatcaatt
      781 aaaaatacgg gcaattcttg taagtgaaat gatgcatatc tattattccg tggaaaaaat
      841 tcaaatataa atcttagtaa agagattctt gtataaaatt aactattttt aaaaatacta
      901 ttggtgtaaa acagaaattt gctgtcaaag cacctgtaag aagaaaggag tacagttatg
      961 aaaacaagac ccttagattt tttgtcatta ttacttatca tcatcggtgc attaaactgg
     1021 ggattaattg gctttttcca atttgacctg gtagccagtt tatttggtgg aacggatagc
     1081 tggttaagtc ggattgttta tgcccttgtc ggactggcgg gtttatacgg attaagtctt
     1141 tttggcagaa ttgatgaaga aagattgggt gaaagagaat aatatttata aaaaaacaac
     1201 aaaaattgac ctttttttaa aaaatgtttt ttaaaaaaag gtttttttaa tctggggaag
     1261 aatatattta atatagaagt tgtacaatta tattaaaggg ggagattcca atgatacaaa
     1321 tcatttcagg agaaaaagga gaaggtaaaa caaaacattt aatcgatatg gccaatacga
     1381 aagtaaaaga aacacctggc catatcgtat accttaacaa cgaccgtgaa cacatgtatc
     1441 agttacacta caagatccgc ttcattgttg tagacgattt tcctgtgaaa aactcaaagg
     1501 aatttttagg atttatatgt ggtatcattg cccaggatca tgacattgat catatttata
     1561 ttgatggatt attgaaaatg accaacctta cactggaaga agcatataat cttattgaag
     1621 aattaagaca agttggtgaa aaattcaatg taaatttcgt aattggaatg agttgtagaa
     1681 ataaagatat ccccgaatcc ttaaaaagtt atttgatcgc gtaaataatt gtaaatgaat
     1741 ataaataaat atatgattta ataacgaacg aaaataaatc ccctaactgg ggattttatt
     1801 tttatcttca ttcatttctt ttttgataat gtcaacaaaa acatctacta ttctcacatc
     1861 aaactggata tttttattat gaattaattg ttccagagca acttctttag gatatggaat
     1921 aatcctgtat tttcttcctg ttgtcattgc atcaaaagca tcagctacgg ccagaatttt
     1981 tgaatacaaa ctaatttcct catcaaccag accatctgga taacctttcc cgtctattct
     2041 ttcatgatgc tgtaaaatag catatttagc attatctaag aagggtaagt ttttgacaat
     2101 ttcatagcca agtcggggat gcatttttat aagttcagcc tcttcttctg ttaattcatc
     2161 ttctttatta agaattcttg aaggaattcc tatttttcct atatcgtgca gtaatgccgc
     2221 aaattctagc tcattgattt catttttact taaatttaat gcttggccta ttttaacgac
     2281 atattccctg actctttcac aatgtcctct ggtatataca tcttttactt ccagtgaatt
     2341 cgttaaagca ttgatggctg caatacaaat atttttattc tcactaaata attcttctaa
     2401 tttttcattt aacatactta tctgttcact ttgactggca ataacgtctt tttgcatttc
     2461 aatttgacta tctttaagca ataattccct ctctttatta catttttctt ctatcaaaac
     2521 tttcatcttc cgggacagat tattaatact ttttcccaac atattaaatt catccgtcgt
     2581 attaatctca atcatgtaat cataattgcc attagatacc tcttcaatac ctatcatcat
     2641 tttctttata ggatttgaga tataaaaaac aatcaaagcc cctaaaacga tagaaataac
     2701 aagaaaaaga acagacataa ttaataaata aaagtcagaa ttgttataat ttgaatataa
     2761 aaaattataa tttgataaac ttacgttagt atctgcatat tttcttaagt tatattgttt
     2821 ttctaatgaa tcatttgtgt taagataaat attaaaggca attaaattaa aagctaaaaa
     2881 aatccaaaaa cttattataa tgcttaattt atatttaaaa gaaattattc tctttttctt
     2941 catgaataat actcctttgt tcagcatact ttattcgtgt aatattttac tatattttta
     3001 tcaagatttg tcctattatt tttaattaat taaattttta acttaagcgt atattttttt
     3061 tagataaaaa gtcatatttc aatgtaaagg agcaaagcaa aatgacaaac gtagcagaaa
     3121 gatttttaaa atatgttaaa tttccaacca gttcaaatga atcctctcca acttgcccaa
     3181 gcacaaaaaa ccaattagag tttgctaaat atttaatttc tgaatgtcag actattgggc
     3241 ttgaagaagt tgaacttgac gaaaatggtt atgtcatggc taccttaccg tcaaatacgg
     3301 ataaagatgt tgaaaccctt ggttttattg cgcatatgga tacaagccct gatatgtccg
     3361 gcgaaaatat ccagcctaaa atgataaaga attataatgg tgaagacatt gttttaaatg
     3421 aagaaaaaaa tatcatttta tctccaaaag ttttccccaa cttaaaaaaa tatacaggtc
     3481 atacccttat tacaacagat ggcaatactt tattaggtgc tgatgataaa gcaggtattg
     3541 ccgaaattct aacggctatg gaatatctta ttgctcatcc ggaaattaaa catggtaaaa
     3601 tccgtattgc ttttacacca gacgaggaaa tcggaagagg tgcagatctt tttgatgttc
     3661 aaaaatttgc ggctgatttt gcctatacta tcgatggtgg attattagga gaactccagt
     3721 atgaaaattt taatgctgcc tactgcaaaa taaccattaa tggtcaaaac gttcatcctg
     3781 gtacggcaaa agaaaaaatg aaaaatgcag tccgtatagg aactgaattg gccaatttat
     3841 ttcccaatga ggaaattccc gaaaaaacag atgggtacga aggctttttt catctgaaca
     3901 gttttaacgg aaatgttgaa aaggctgaat taacttatct aatccgtgat tttgatatga
     3961 cgcattttaa gcaacgaaaa gaatttatag aagatacagt cagaatgatg aatgaacgtt
     4021 atggtcaagg caccatacac ctggatttaa gagaccaata ttataatatg aaagaaagac
     4081 tggaaagcaa aatggacatt gtaaacagag cttttaatgc tatgaaagcc gtaggcatag
     4141 aacccgtcgt aagacctata agaggcggta ctgacggagc ccgattatct tttatgggcc
     4201 tgccctgccc taaccttttt acaggcggtg ataactttca tggtaaatat gaatatattt
     4261 ccgttgaagc tatggaaaaa gccgtagaag ttatcgttaa aattgcagaa atatagattt
     4321 tattttaatc acacttgagt aaattttaga attatggtat gatatcttac aataattaag
     4381 ataaaagtga tgaaggagac caagtagtcg tcacggttac aaaatcagag agcaaaccgg
     4441 ctggtgaaag gtttgtagta cgtgaaccgg cgaagtacaa ctcccaagct tcggtttaac
     4501 cgacggttct ctccgttaaa gagcagaggc atgaatgatt tcatgcgaat taaggtggta
     4561 ccacgggtcc tctcgtcctt ataaaatgtt ttatacagga gagaggcttt ttttaggtaa
     4621 aaatttaata ttatacgaag ataaacttaa agattggagg atattatgaa caacgttttt
     4681 gatgttttgc aagaaagagg ctttattcaa caaacaaccc atgaaaaaga aataagagaa
     4741 ttattagaaa aagaaaaagt tactttttat attggttttg accctacggc agatagttta
     4801 cacgttggtc attttgtaac cattatggcc atggcacata tgcaaaaagc tggtcacaga
     4861 cctattgcgc ttataggtgg tggcacagca atgattggtg accctactgg taaaaccgat
     4921 atgcgcaaaa tgatgacaga agaaaccata caacataatg cagaatgttt taaaaagcag
     4981 ctttccagat ttattgattt tagcaacgat aaagccatta tggttaataa tgccgactgg
     5041 ctgcttaaat taaactatat ttctttctta agagaaattg gcgttcactt ttctgtaaac
     5101 aaaatgctta cttatgaaag ttataaaacc agaatggaaa aaggactgtc tttcttagaa
     5161 tttaactata ctctcatgca gtcttatgac tttttagaat tgtacagaag atacaactgt
     5221 aaacttcaat taggaggaaa tgaccaatgg tcaaatattt taggaggtgt tgaactaatc
     5281 cgacgcattg gacatgacga tgcttttggc atgaccttta ccctgctgac aaccagcgaa
     5341 ggtaaaaaaa tgggtaaaac cgaatccgga gccatttggc ttgatcccca aaaaacatct
     5401 ccttatgatt tctatcaata ttggagaaac gttgccgatg cagacgttaa aaaatgctta
     5461 tcacttctta ccttccttcc tatggaagaa gtaaaccgtt tagcagcttt agaaggggct
     5521 gaaattaata aagccaaaga aatccttgct tatgaagtga ctaaaattgt gcatggtgaa
     5581 gaagaagcta taaaagctga tacagctgcc aaagccttat ttaaaggtgg cgctgaagga
     5641 ggttccattc caacaacaga aattaccaaa gaagaattgg taaaaggcat ggatatcttg
     5701 accttacttc aaaaaacaaa tcttgttcct tcccgttccg aagggcgtcg tttaatacaa
     5761 caaggcggaa tcaaagttca aaatcaaaaa atcagtgata ttgattatct tattacagaa
     5821 aaagactttg aaaatagtag cttaatgatt caaaaaggta aaaaagtctt tcaccgtgtt
     5881 caattaatat aagtaaatca tctgtttatt atttataata aaattcagct aaaatataaa
     5941 ccccatagaa aaactttcta tggggttttt tcttcacttt ttctttccat aaagcggatt
     6001 aataagagtt tcaggaaagc tgcaataggt actgcaataa gcattcccaa caatccaaat
     6061 aaagaacctc caattaatat ggataccaca attgctacag gatgaacatc aacactttcc
     6121 cctacaacct taggtgcaat gattgcccca tcgatttgct gtaagataaa aagggttatg
     6181 caagcataaa gtgctttcat cggattgtca ctgattaaac ccataaacgc tgccggaatc
     6241 atgccaataa ttggtccaaa ataaggaatt acattggcta tccctgcaat gattccaata
     6301 ataatcggga aatctattcc aataattgcc acacttattc caattaaaac acccatgatt
     6361 aaagaatcta ttaattgacc tcttatatat cccgataaaa cggtatccat atcaatacaa
     6421 agatatttta atcgaatata aattttttct ggtaacaaga tcctgaaaaa ttgattaaag
     6481 actcttaata acttggcttt atcttttaat aagtaaaatg caataacaaa agctaagacc
     6541 aagtcaacaa ccctgtttcc cgcctttgta atcttttcaa taaaaaataa ggcaatattt
     6601 tgaacataat ttcctaatct accactaaac tcagtcaggg tttcttctgc cttttgtaaa
     6661 aatccaatat cctcaagccc ttgttgcaaa tttgttatca tattattaaa ggattggacg
     6721 taacttttga tgctgcttac caaattgtct aaatttttaa aacctttatg attacccaag
     6781 cttttggata ataatatccc tataattgta agtgctaaaa taagcgtcaa atatgtcaga
     6841 agtgtaggaa gaaatcttga aatctctttc tctttatcct gacttttttc attattatcc
     6901 tttttgccaa aaataatatt tttatctttc aaaaatttcc ctaaaggctc tacaactctt
     6961 ctctgataga tatcaaccat tggatccaaa agataggcaa tcgctactcc aataaaaaaa
     7021 ggagacaaaa tggataatat cctggaaatc attgcagcaa tcgttgctgt aataggaata
     7081 atgttataaa taaaaatagc cagtccaaaa ataactatca gtgtaaaaat tacatgaaat
     7141 gaaattttta aataatgctt atcccaaggt aatttcacct agatcccatc tttcttatta
     7201 ttagagattt gttgttattt gcattttaat gtaatattac gattattata tacagatatc
     7261 cgaagacttg taaagctatt ttatttcttt taatacagca agcaataact cccaggtatt
     7321 tttagtagat gaaatactta atctttcatt gggcgtatga atgtctttca tattaggacc
     7381 taaagaaata atgtcaacat cttttatttt ttctgcaaaa aagccgcatt cgagtcctgc
     7441 atgaatagct gttatttcag cttttttacc aaataattct tcatacttat tagtaaagaa
     7501 tgaacggatt tttgagtcct ctgcatattg ccaggctgga tattcaccct ttagcaaaaa
     7561 gtcggcacct acaaaacttg ccagaacttt aattttatta atgataagcc ttttcctgct
     7621 ttctacagaa cttctcaatg ctgatataaa acgtatttct tctttgtctg taataacaac
     7681 ccccagattc gttgaacttt ctaccaaacc ctctatatcc atactcatac tttggacacc
     7741 attgggcatc agcatgagta tatgaataat ttttcccttg atttcttctg ataaatgttc
     7801 actacttatt tcccttacca gttctatctt gacttcaata tccgggtcag actttttata
     7861 ttcatttttt aaaatatcgt tccacttttc tattatcttc ataagctttt ctatattttt
     7921 agattcgatt tgaatgaccg catcggcttc ccttggaata gcattatctt ttgccccgcc
     7981 attaatggat attaaatcaa aatgcatatc ctcttgcagg ctgtataaaa tccgtcccat
     8041 taataaattg gcattagcac gttctttgtc aatttcctga ccagaatgac caccttttaa
     8101 tcctctgatt tgaatggaat aaacaggtcc tgatttttct tctttaaccc attgcgtttt
     8161 taagattaaa tgtgccctgg caccacctgc gcaactggaa agaaaaaccc cttcttcttc
     8221 tgaatccaga ttgattaata ttttgccctt taaatgagaa caatctaaat ttgcagcacc
     8281 ttccatcccc gtttcctcac tggttgtaaa gacaacttct aaaggaggat gaggaatatt
     8341 atccgatgct aaaagtgcca gtccataagc cagagctata ccatcatctc ctcccaggct
     8401 ggtacccctg gcaaaaatgt aatcgccttc tacgactaaa tcaagcggat ctttcaaaaa
     8461 gtcatgtacg gtatctttat ttttttcaca aaccatatcc atatgtcctt gtaagataac
     8521 cacaggcgca ttttcatagc caggactagc tttctttttt ataattacat tcaaggcttt
     8581 gtcctgtatt acttctaatt gatgctggcg ggcaaattcg actaaataat cactaattgc
     8641 tttttcattt cctgagcctc ttggaatttg actaatgctt tcaaaatact cccacaccat
     8701 ctttggttct aattctttca cggttgaagc catatttatt tcctttcttt tattatttta
     8761 aaaaaaccaa gccctttata gtttagactt ggtttaatat aaatataaca tattctctta
     8821 atttttaaaa ctatgaactg gcgcaggaat ccggcctcca cggttaataa aatctttaca
     8881 gctaaacgaa ttcactgcca tgataggtgc atagcctaat aagccaccaa attcaaccac
     8941 atctccaaca ccttttccaa taacaggtat gatacgaact gctgttgtct ttccgttgac
     9001 catgccaatg gccatttcat cagcaataat tcctgaaatc gtttcttcac tggtatctcc
     9061 aggtattgca atcatatcaa gacctacaga acatacgcaa gtcatagcct caagtttttc
     9121 aaggttgata gagcccttct caacagcttt aatcataccc tggtcttcac tcacagggat
     9181 aaaagcaccg ctaagacctc ctacataaga agaagccatg attccgcctt tttttacatt
     9241 atcatttaat aaggccaaag cagcagttgt gcctggtgca cctacggatt ctaagcccat
     9301 ttcttcaagg atttctgcaa tactgtctcc aatagcaggc gtcggagcca aagacaggtc
     9361 aatgatgcca aagggaatat taagcctttg tgaagcttcc ctggcaacca actgtccaac
     9421 ccttgttatc ttaaatgctg ttcttttaac ggtttcgcaa agggtttcaa aatctttgtt
     9481 ttttacttct tccaatgctt tttttaccac acccggacca cttacaccta cattaattac
     9541 tgcatctttt tctcccacac cgtggaaagc gcctgccata aaagggttgt cttctggtgc
     9601 attacaaaat accacaagtt ttgcgcaacc gatggaatct ttatctttcg ttttgacagc
     9661 agtatccttt ataattttac ccatttcttt gaccgcatcc atattaattc ctgttttact
     9721 actgcctaaa ttcactgaag aacaaaccct ctcagtctca gccagagcct gtgggattga
     9781 ttggataagt acattatcag aagcattgca acctttatga actaaggctg aaaaacctcc
     9841 aataaaatta acccccactg cttttgcagc cctgtccagg gtatgagcaa caggaacata
     9901 agattgggca tttgttgcag ctgcaacaat ggaaataggt gttactgaaa tccttttgtt
     9961 aacaatgggg ataccaaatt ctttttcaat atcttctcca actttgacta aatgctcagc
    10021 cgtttttgta atcttatcat aaattttccg atttagcgta tccatattgc tgtcagcaca
    10081 atcgagtaaa ttgataccta aagtaatggt acgcacatct aatttggctt gttcaatcat
    10141 ttgatttgtt tcctggactt caaatcgcgt aatcatctat tccctctctc cttggtgatt
    10201 aaattctatg catactgtta aaaatttctt ccctttggag tttaattaca accccgatct
    10261 gctttcctaa ttcgataagc tcatcttcga ttttaccaaa gtcatctgtt gcattcgtta
    10321 aatcaacaat catcatcata ttaaaataac cttgaacaat tgtctgggaa atatctaaga
    10381 tatttatgtt tttttcagaa agcaaggtgc agacttttgc tataattcct accttgtctt
    10441 ttccgacaac agtaataatt cctttcattt cttaaatgct cctttcatct gctgaacatt
    10501 tttcattact catctaatga ttaaaatgtt tttacaattt tagcacattg tttgttcttt
    10561 atcaatgttt aattgtccat ta
//
LOCUS       DAOSUD010000068        10231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3638_length_10231_cov_24.811517, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000068 DAOSUD010000000
VERSION     DAOSUD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3638_length_10231_cov_24.811517"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..418)
                     /gene="spoVG"
                     /locus_tag="PLG49_08495"
     CDS             complement(95..418)
                     /gene="spoVG"
                     /locus_tag="PLG49_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOQ17395.1"
                     /translation="MEITDIRVRKINKEGKMKAVVSVTFDNEFVVHDIKVIEGDKGRF
                     IAMPSRKTMDGEFRDIAHPINSATREKIQTAVLEKYEMILVADENDAAEEVAAAEEAN
                     DGENE"
     gene            complement(561..1667)
                     /gene="glgD"
                     /locus_tag="PLG49_08500"
     CDS             complement(561..1667)
                     /gene="glgD"
                     /locus_tag="PLG49_08500"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOQ17396.1"
                     /translation="MKALGIILAGGSSETLSDYPNKSIAALPVGGSYRTIDFTMSNMT
                     NSGVNKVAVITQYSSRSLLDHLSSAKWWDLGRKQGGLFVFTPYITNDNPFGYRGTADA
                     MYRNISFLKRSNEPFVIIASGKHVYKIDFNDVLEYHQQKQADITIIYKEANYEKPSNY
                     GVMVLDENKRLIEFEEKPLEPQTNIISLGIYVIPRTLLIKLLEDVVAENRYDFVNDVI
                     IRYRKKLKIYGYPFEGYWRCIRSIQSFFDINMDFLNKDVRDLFCKQYPYIATKVMDEP
                     PAKFNIGSSTKNCIVGGGSILNGYVENAVLFRKVFTGSYSFIKNAIIMDGSYIGNNCY
                     LEYVVLDKEVVVSDGRKLVGSKENPIFLEKGSVI"
     gene            complement(1664..2941)
                     /locus_tag="PLG49_08505"
     CDS             complement(1664..2941)
                     /locus_tag="PLG49_08505"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOQ17397.1"
                     /translation="MRKREIISMILAGGHGTRLGILTKHLAKPAVTFGGKYRIIDFVL
                     SNCINSDIDTVGVLTQYEPLKLNQHIGIGIPWDLDRKTGGVTILPPYIKEGQGAWYSG
                     TANAIYQNIKFIEEYNPEYVLILSGDHIYKMDYSKMLEYHKQKEADATIAVLEVPIEE
                     AHHFGIMNTNEEDRIVEFEEKPREPRSNLASMGIYIFNWNKLKQVLIEDNEYHPDSDF
                     GKHIIPRMIHNEDRLFAYRFNSYWKDVGTIESYWQANMELIETLPSFNLYEEFWKFYT
                     NLDHQPPQYVGEAADVKRSILSEGSEVYGNVYSSVLGSGVIIEEGATVRDSIIMAGTI
                     VQSGSVLNRCIVSENCFIGKNVIIGEGENIANFEKPDIYHTGITVVGDQTTVPDNVWI
                     GKNCVVYGNTKFSDYNNGRLESGQSLILEGEES"
     gene            complement(3138..3947)
                     /gene="purR"
                     /locus_tag="PLG49_08510"
     CDS             complement(3138..3947)
                     /gene="purR"
                     /locus_tag="PLG49_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HOQ17398.1"
                     /translation="MNRINKNERVGAIIKILTENPKKIYNLNYFCEIFDCAKSTLSED
                     IDIVKDIFEKFKLGEIETVTGAAGGVLYKPNMTKEQFKDFCTELCKKINNSERIIPGG
                     YIYMNDLIYSADVSFNIGRALGSFFQDVDFDYIVTIETKGIPIALMTARVLNKPLVVV
                     RKEGRLTEGTAIHMNYVTGSSKRIQTMYLAKRSIARDSKVIFIDDFMKAGGTAKGIID
                     LMKEFEAEVVGVGILMVTKEPEEKLIDNYHSLIVLEEIDSSSGEIIVKPSI"
     gene            4249..5691
                     /gene="murC"
                     /locus_tag="PLG49_08515"
     CDS             4249..5691
                     /gene="murC"
                     /locus_tag="PLG49_08515"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOQ17399.1"
                     /translation="MSKINFNDTNKKFHFIGIGGISMSGLAEILLNKGFKVSGSDIKN
                     TKIIKHLEDLGVDFYLGHLASNIKDDVDFVIYTAAIKKDNPELVEAQKRNLTILNRAE
                     LLGEIMKAFPYSIAVSGTHGKTTTTSMLSNILIQAKKDPTVTVGGIVDAIGGNIRIGQ
                     SEYFITEACEYCDSFLNFYPYIGIILNMEEDHLDFFKDINHIRKSFTAFAKNISKDGA
                     LIINGDIENLSLILDEIDCKVVTFGTDPSRVMWTAANIKYNEKACGKFDLLYQGKNMG
                     TVSLNVPGIHNIYNALAASAAAFELNIEIADIIKGLHNFKGTHQRFEIKGSLKGITIV
                     DDYAHHPTEIKATLEVAKHYPHNTIWCVFQPHTYTRTKMFLKEFAEALKEADKIILTD
                     IYAAREKDLGEIHSKDLQRELAKIGKESYYFPDFDSIENFLLQNCIPGDLLITMGAGD
                     VGTIGEDLLGANISTLSTNFPVEKEIHSCE"
     gene            5947..7038
                     /locus_tag="PLG49_08520"
     CDS             5947..7038
                     /locus_tag="PLG49_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOQ17400.1"
                     /translation="MTVIHFENRTDPATTPIHNEFIDKYLPKADGNFVKVYIFLYRCY
                     YHQKPSLSTKDISEKLGLLESDVIKAFQYWAEQGLIHFKQNDDMITIGFQIPSDKEIK
                     EDSPKNSVHKKVHIYHETRPNYSPEELSIYMKNPEISQLFKIAERYLGRMLNHNDLQI
                     LYSLYDWLRLPVDVIELLLEHCISNNHRSIRYIEKVAITWAEEGINTLEKAKLRIKIF
                     NEDFREIMKTFGLNRDPAPVEIEYMNKWINELHAPMEIILEACKRTITQIQEPKFSYA
                     HTIIDSWNKNGVKSLEDIKKLDAAYLQKKNLQNNSSNTNDYYSKPSKFVNFKQREWDF
                     EELERLEREYLQKELSKEDDCSEIKKNFF"
     gene            7084..8079
                     /locus_tag="PLG49_08525"
     CDS             7084..8079
                     /locus_tag="PLG49_08525"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ17401.1"
                     /translation="MSIRESQYKKILKDYELKRERSNKELRQRQEEVYKKIPRIAEID
                     KLLASTGIEISKAILQNPEKSEELIHSLEQKNLDLMIEKAELLYINGYNKNYLEPSYE
                     CYSCKDTGYINNKPCHCFKQALIDIAYDQSNLKEILAVENFDNFNFDYFSTEIDPESN
                     ISPRENIKQIYSHCIRFIEQFDEQFDNLIFYGNPGLGKTFLCNCIAKDLLDRGKTVIY
                     LTAFQLFQLFEQARFNKDENDEFTKDYLESIFTVDLLIIDDLGTEFNTSFTGSELFNC
                     LNSRLLDKKHTIISTNLSPNDWQKQYSERIVSRIFGHYKALKLIGNDIRILKKYR"
     gene            8174..8872
                     /locus_tag="PLG49_08530"
     CDS             8174..8872
                     /locus_tag="PLG49_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOQ17402.1"
                     /translation="MNSFRKQFLLLFILALVLCDMVYYRHSYMHLYQGVFQQNKNPYY
                     IHIDLDDYKMYVFKDDEIIKIYPVSGGKQDSPSPLGTWTIISKADWGEGFGGTWMGFN
                     VPWGMYGIHGTDEPWSIGRPLSQGCIRMYNKDAKELQKVIPHGTKVTIVKGSFGPFGD
                     YFRVLEPGDTGSDVLMVQRKLNDLGYYNGYCDGKYGDHMKTAIHKFQKDNNLPVNNHV
                     SHAMYEKLGFIPME"
     gene            complement(8902..>10231)
                     /locus_tag="PLG49_08535"
     CDS             complement(8902..>10231)
                     /locus_tag="PLG49_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ17403.1"
                     /translation="KQLEESKQNVMDEVEVNGNKYEVLYKPILDENQELIGHFSVWVL
                     KEDVIDMSNAILNKLLLTIIIAIVLGIIAALFFGTVIHKALSEVVKDVTKISNGDYMI
                     NTNKYKINRNDEVGELSRAIVDMINKQTSVLKYVSENANNMDTSSKELHETVETIMAQ
                     IEEISSATEDIVAVMEEVDAASEEVHNTSVAVVDNINKLYENINQSKGTAEQIKNRAE
                     SMKDASQKSQEETSTIYVQKKEKILEGIKKGEIVKEITNMAQLISSIAEQTNLLALNA
                     SIEAARAGESGRGFAVVAEEIRTLAEQSANTVTNINTVITQVYEAFENLSDNARDILE
                     FINEKVNKDYELMLETSMQYQRDSELVNELVAQFVESTQQINRSVEEIKKQIQEVSKA
                     INSATLNTQNIAFGLKETTAAVESIEEVAARQSQLGDKLTGLLGEFTLAN"
BASE COUNT         3398 a         1874 c         1505 g         3454 t
ORIGIN      
        1 cttgctaaaa taaaactttt aattcaattt acggtaagcc ctatatgacc aaattaagaa
       61 aagcacttcc taagaagtgc tcaattacca gctattattc gttttcccca tcatttgctt
      121 cttctgcagc agcaacttct tctgccgcat cattctcatc tgctacgaga atcatttcat
      181 atttttccaa aacagcggtt tgaatttttt ctcttgtagc agaattaatt ggatgagcaa
      241 tatctctgaa ctctccatcc attgtctttc gacttggcat tgcaataaat cgacctttgt
      301 ccccctcaat tactttgata tcatgaacaa caaattcatt atcaaaagta accgatacaa
      361 ctgctttcat cttaccctct ttgttgattt tgcgcacacg aatatctgta atttccatac
      421 gtgaacccct ctctgtaaca tgatgcggct ctagagcaag ttctagacac cgttaaattt
      481 gtacttctat tatataacag atttatccaa atatcaatat gttttatata ttcaaattat
      541 acaaaattat atcaattata ttatataaca ctacctttct ccaaaaaaat tggattttct
      601 ttacttccaa ccaattttct tccgtcagaa actactactt ctttatctaa aacgacatac
      661 tccaagtaac aattatttcc aatatatgac ccatccatta tgatggcatt tttaataaag
      721 gagtatgagc cagtaaatac ctttctaaat aaaacagcat tttcaacgta tccgtttaaa
      781 atcgaacctc ctccgacaat gcaattcttt gttgaagaac caatgttaaa cttagccggt
      841 ggttcatcca ttactttagt cgcaatatag gggtattgct tacaaaataa atccctgaca
      901 tccttattta aaaaatccat attgatatca aaaaaagatt gtatagatct tatgcatctc
      961 caataaccct caaaaggata accatatatt tttaattttt ttctatatct tataataaca
     1021 tcattgacaa agtcataacg gttttcggca accacatctt ctaaaagttt tatgagcaaa
     1081 gttcttggaa ttacatatat accaagagaa ataatatttg tttggggctc taaaggcttt
     1141 tcttcaaatt ctataagcct tttgttctca tccagcacca taaccccgta attagatggt
     1201 ttttcataat tagcttcttt gtaaataatg gtgatgtctg cctgcttttg ctgatgatat
     1261 tccaatacat cattaaaatc aattttatag acatgttttc ctgaagcaat aataacaaag
     1321 ggttcattac ttctttttaa gaagctgata tttctgtaca tcgcatcagc agttcctcta
     1381 taaccaaaag gattatcatt ggtaatatag ggagtaaaaa caaagagtcc tccttgcttt
     1441 ctccctagat cccaccattt agccgaagat aaatggtcta ataaagaacg ggaactatac
     1501 tgcgttatta ctgctacctt attgacacca gaattcgtca tattgctcat tgtaaaatct
     1561 atcgtcctgt aggaccctcc aacgggtaat gcagcgatgg atttattggg ataatcgctt
     1621 aatgtttcac tacttcctcc cgccaatatg atacctaatg ccttcatgac tcctcccctt
     1681 ccaaaataag agattgcccg gattcaagcc ttccattatt ataatcacta aactttgtgt
     1741 tgccataaac aacacaattc ttgcctatcc acacattatc cggcacggta gtttgatctc
     1801 caacaaccgt aattcctgtg tgatatatat caggcttttc aaaattagct atattttctc
     1861 cttctccaat aatcacattt tttcctataa aacaattttc tgagacaata caacggttaa
     1921 gtacagaacc gctttgcaca atcgtaccag ccattatgat tgaatccctt acggtagctc
     1981 cctcttcaat aataacacct gatcccaaaa cagaactata aacatttcca taaacttcac
     2041 tgccctcaga taaaatactt ctttttacat cagcagcttc tccgacatat tgaggaggct
     2101 gatggtctaa gttggtataa aatttccaga attcttcgta caaattaaaa cttggaaggg
     2161 tttctataag ttccatattg gcctgccaat aagattcaat tgttcctacg tccttccaat
     2221 agctgttaaa tctataggca aataacctgt cttcattatg aatcattcgg ggtataatat
     2281 gttttccaaa atcactgtcc ggatggtatt cattgtcttc aatcagcact tgttttaatt
     2341 tattccagtt aaatatgtaa atacccattg aagctaaatt actcctgggc tctctaggct
     2401 tttcttcaaa ctctacaatt ctgtcttcct cgttggtatt cattattcca aaatggtgag
     2461 cttcttctat aggaacctct aatacggcga tggttgcatc agcttctttt tgcttgtgat
     2521 actccaacat tttgctataa tccattttat aaatatggtc acctgaaaga ataagaacat
     2581 attccggatt atattcttca ataaatttta tattttggta aatggcatta gctgttccag
     2641 aataccaggc accctgcccc tctttaatgt aaggaggtaa aatcgtaaca cccccggttt
     2701 tcctgtctaa atcccatggg attccaattc cgatatgctg gtttaatttc aaaggctcat
     2761 attgcgtcaa tacaccgacc gtgtcaatat ctgaattaat gcaattactt aaaacaaaat
     2821 caataatcct gtatttgcct ccaaaagtga ctgccggttt agccagatgc tttgttaaaa
     2881 tacctaagcg agttccatga ccgcctgcca gtatcattga tataatctct ctttttcgca
     2941 tactccccac cctcttgatt gttcttgcct gtaatattag atttctaatc tgtatttgaa
     3001 gataaaaatt aactaagttc aaaagtttcc tgtatattaa taatagcata ttcaaacata
     3061 tttgaacaat ccaaaaaatc tagtcaatta tcactattta ttataaaata aacaaaaaaa
     3121 atagcctatc ggctatttta tatagaaggt tttacaatta tttctccgga actactatca
     3181 atttcctcca aaactatcaa actatgataa ttatcgataa gtttttcttc aggttctttt
     3241 gtgaccatta aaattccaac tccaacaact tctgcctcaa attctttcat taaatcgata
     3301 attccttttg cagttcctcc agctttcata aaatcatcaa taaaaataac ttttgaatct
     3361 ctggcaatag atctctttgc cagatacatg gtttgaattc ttttagaaga acctgttaca
     3421 taattcatat gtatagcagt tccttctgtt aaccttcctt cttttctaac caccactaaa
     3481 ggtttattaa gtactctggc agtcattagg gcaataggta taccctttgt ctcaatggta
     3541 acaatataat caaagtctac atcctgaaaa aagctgccta aagccctgcc aatattgaaa
     3601 ctgacatccg ctgaataaat aaggtcattc atataaatat atccgccagg aattattctt
     3661 tcactattat ttattttttt gcaaagctct gtacaaaaat ctttaaattg ctcttttgtc
     3721 atgttgggct tataaagaac accacccgca gcaccagtta cggtctcaat ttcacccaat
     3781 ttaaattttt caaaaatgtc ttttacaata tcaatatctt cgcttaaagt agatttagca
     3841 cagtcaaata tctcgcaaaa ataatttaaa ttataaattt ttttggggtt ttccgtaaga
     3901 attttaatga ttgctccaac cctctcattt ttattaattc ggttcatttt atactccttt
     3961 cattgactgg aggattcatt tttattatta tataccaact catcgaatat tttaatattt
     4021 ttttaaaaaa atattcggtt ttatttttct tattctatac tgcttttatt tttagaattt
     4081 aaatttatta ctttttataa taataataat atagtataat aatatattta gaaaaataaa
     4141 tataaaaata tgtttttcaa ttttatgtat aaattattca atcttatccg aatatatttt
     4201 tatcagcata tttaactgcc ttaaaaatag aacgaattga ggtgtagtat gtcaaaaatt
     4261 aattttaatg atacaaataa aaaatttcac tttattggta ttggcggaat cagtatgagt
     4321 ggtctggcag aaatcctttt aaataaaggt tttaaagttt ctggctctga cataaaaaat
     4381 acaaaaatca taaagcattt ggaagattta ggtgtagact tttatttagg tcatttagct
     4441 tcaaatataa aagatgatgt ggatttcgtt atttatacag cagctattaa aaaggacaat
     4501 cctgaattag tggaagctca aaaaagaaat ttaactattt taaaccgtgc tgaactgctc
     4561 ggggaaatca tgaaggcttt cccttattct atagctgttt ccggaacaca tgggaaaact
     4621 acaacgacat ccatgctgtc taatatcctg atacaagcta aaaaagaccc tactgttacc
     4681 gttggaggca ttgtagatgc cattggtgga aatatccgga ttgggcaatc agaatatttc
     4741 attacagaag cctgtgaata ttgcgatagc tttcttaatt tttatccgta tatcggtatc
     4801 atattaaata tggaagaaga ccatttggac ttttttaaag atatcaatca tatcagaaag
     4861 tcttttacag cctttgcaaa aaatatatct aaagatggag cccttattat taatggtgac
     4921 attgaaaatc tttctcttat tttagatgaa atcgactgca aggttgttac ttttggtaca
     4981 gatcccagcc gcgttatgtg gactgctgcc aatattaaat ataatgaaaa agcttgtgga
     5041 aaatttgact tattatatca aggtaaaaat atggggacag tttctttaaa tgtccctgga
     5101 attcataaca tttataatgc acttgctgct tctgctgcag cctttgaatt aaatatagaa
     5161 atcgccgata ttattaaagg actgcataat tttaaaggca ctcaccaaag atttgaaata
     5221 aaaggcagtc tgaaaggtat tacaattgtt gatgattatg cacaccatcc cacagaaata
     5281 aaagcaaccc tggaagttgc aaaacattat cctcataata ctatctggtg tgtttttcaa
     5341 ccccatacct atacaagaac taaaatgttc ttaaaagaat ttgcagaagc attaaaagaa
     5401 gcagataaaa tcatcttaac tgatatttat gctgcgcggg aaaaagattt aggagaaata
     5461 cattcaaagg atcttcaaag agaactggct aaaatcggaa aagaatcgta ttattttcca
     5521 gattttgatt ccatagaaaa ttttctttta caaaattgta tccccggcga cttgttgata
     5581 actatggggg caggagacgt tggtacaatt ggggaagacc tgcttggagc aaatatatcc
     5641 acattatcca caaattttcc tgttgaaaaa gaaattcatt catgtgaata aattcttaat
     5701 aactatagtt tatcccatat ttttacatcg ttttttatac ccaaaaagta aaactttttg
     5761 ggtattttac atttaaaaag agttatttat tcaatttatc cacattatcc acagaattat
     5821 taacataatc tatgttaatt atactttatt ttgattaaga aagtctgaat tttctttcct
     5881 aaacaatcca taaactatgt gttataatat caatacaatt aagcttaaaa gaaaggagag
     5941 atggtaatga cagtgattca ttttgaaaat cgtacggatc ctgctacaac tcctattcac
     6001 aatgaattta tcgataaata tctgccaaag gccgatggaa attttgtaaa agtatatatt
     6061 tttttatacc gctgttatta tcatcaaaaa ccatctctca gtaccaaaga tatttcagaa
     6121 aaattgggct tgttagaatc agacgtcatt aaagcctttc aatattgggc agagcaaggt
     6181 ttaattcatt ttaaacaaaa tgatgacatg attacgattg gtttccaaat accttccgac
     6241 aaggaaatta aagaggatag tccgaagaac tccgtgcata aaaaagtaca tatttatcat
     6301 gaaacaagac caaattatag ccctgaagaa ttatccatat atatgaaaaa tccagaaatc
     6361 tcccaactct ttaagattgc agaaagatat cttggcagaa tgcttaacca caatgatttg
     6421 cagatacttt acagcctcta tgactggtta agactgcccg tagatgtgat agagctctta
     6481 ctggagcatt gcatatccaa taaccacaga agtattcgtt atatcgaaaa agtagccatt
     6541 acatgggcag aggaaggaat taatacttta gaaaaagcaa aactaagaat aaaaattttt
     6601 aatgaagatt tcagagaaat catgaaaact tttggtctta acagagatcc ggctccagtt
     6661 gaaatagaat atatgaataa atggatcaat gagcttcatg cccctatgga aatcatttta
     6721 gaagcatgta aaagaaccat tacgcaaatt caggagccta agtttagtta tgcccataca
     6781 atcattgaca gctggaataa aaatggtgtt aaatcattag aagatataaa aaaactggat
     6841 gctgcatatc tgcaaaagaa aaacctgcaa aacaattctt caaatactaa tgattattat
     6901 agcaaaccca gtaaattcgt aaactttaag caaagagaat gggacttcga agagttagag
     6961 cgcttagaaa gagagtattt gcagaaagaa ctttctaaag aagatgactg tagtgaaata
     7021 aagaaaaatt tcttttaaaa agattcctga cacaaattct taaatagtag aaggatgata
     7081 cgaatgagta taagagaaag ccagtacaaa aaaatattaa aagattatga gttaaaaagg
     7141 gaacgctcta ataaagaatt aagacaaaga caggaagaag tttataaaaa gatcccacgt
     7201 attgcggaaa ttgacaaatt attagcttca acagggatag aaatctctaa agcaatttta
     7261 caaaatcctg aaaaatctga agagcttatc cattcccttg agcaaaaaaa tttagattta
     7321 atgatagaaa aagctgaact tttatatatc aacggatata ataaaaatta tttagaaccc
     7381 agctatgaat gttatagctg taaagatacc ggttatatta acaataaacc gtgtcattgt
     7441 tttaaacaag cccttatcga tatcgcatat gaccagtcta acttaaaaga aattttagct
     7501 gtagaaaatt ttgataactt taactttgat tatttttcta ctgaaataga tccggaaagt
     7561 aatatttcac cacgggaaaa tattaaacag atatacagcc actgtatccg ctttatagaa
     7621 cagtttgatg agcaatttga caatcttatt ttttacggca atcctggtct tggcaaaact
     7681 ttcctatgca attgtattgc caaagattta ttagacagag gtaaaaccgt catctactta
     7741 acggcttttc aactctttca gctttttgaa caagccagat ttaataaaga tgaaaatgat
     7801 gaatttacaa aagattattt agaatctatt tttacagtag acttattaat tattgatgat
     7861 ttaggcacag aatttaatac atcctttaca ggttccgaat tgtttaattg ccttaatagc
     7921 cgtttattag ataaaaaaca tacaatcatc tctacaaact tgtcacctaa cgactggcaa
     7981 aaacaatatt cagaaagaat cgtctcaaga atttttggcc attataaagc actcaaatta
     8041 ataggaaatg acatacgaat tcttaaaaaa taccgttaaa gaccaaattg atttggtctt
     8101 tttttgttct agtaagctct atttctttca tatcatataa tgtaaatgac aggcggaaag
     8161 aaggaggatt atcatgaatt cttttcgaaa acagttttta cttcttttta tcctggcctt
     8221 agttctttgc gatatggttt attaccgtca tagttatatg cacctttatc agggcgtttt
     8281 tcagcaaaat aaaaatcctt attacataca tattgatctg gatgattata agatgtatgt
     8341 gtttaaagat gatgaaatca ttaaaattta tcctgtttca ggcggaaaac aagattctcc
     8401 ctctccttta ggaacctgga ccattatttc aaaagcagat tggggagaag gctttggagg
     8461 aacctggatg ggcttcaatg ttccctgggg aatgtacggt atccacggaa cagatgaacc
     8521 ctggtctatt ggaagacctc tgtctcaggg ctgcattaga atgtacaata aggatgctaa
     8581 agaactccaa aaagtgattc cccacggaac aaaggttact attgttaaag ggagctttgg
     8641 tccctttgga gattatttcc gagtattaga accgggcgat acgggctcgg acgtcctgat
     8701 ggttcagagg aaactcaatg atttgggata ttataatggc tattgtgacg gtaaatatgg
     8761 cgatcatatg aaaacagcta tacataaatt tcaaaaagac aacaatctgc ctgtcaacaa
     8821 tcatgttagt catgctatgt atgaaaagct gggctttatt cctatggaat aaagcccagc
     8881 ttttcatatc gtctgctttt cttaatttgc aagagtaaat tcccctaaaa gaccagttaa
     8941 tttatcaccc aactgtgact gtcttgccgc gacctcttca atactttcta cagcagcagt
     9001 cgtttctttt agtccaaaag caatattttg agtatttaac gtcgcagaat taattgcttt
     9061 agaaacttct tgaatttgtt ttttaatttc ttctacagaa cggttgattt gctgggtact
     9121 ctcaacaaac tgtgcaacta attcattaac taattcagag tctctttgat actgcatact
     9181 ggtttctaac attaattcat aatctttatt aactttttca ttgataaatt ccagaatatc
     9241 cctggcattg tccgatagat tctcaaaagc ctcatatacc tgtgtaataa cggtattaat
     9301 atttgtaacc gtattcgctg actgctctgc caatgtcctg atttcttcgg ctacaaccgc
     9361 aaaacctctt ccgctttccc ctgccctggc agcttcaata cttgcattta aagccagaag
     9421 atttgtttgc tcagcaatac ttgaaataag ttgggccata ttggtaattt ccttaacgat
     9481 ttctcccttt ttgatccctt ccaggatttt ttctttcttt tgaacataaa tggtgctggt
     9541 ttcttcctgg gatttttgag aagcatcttt catggattct gccctgtttt taatttgttc
     9601 tgctgtgccc ttactttgat tgatattttc atataactta ttaatattgt ctacaacagc
     9661 aacactggta ttatgaactt cttcactggc agcatctact tcttccatca cagcaacaat
     9721 gtcttcagta gcagaactga tttcttctat ttgagccata atcgtttcta ccgtttcatg
     9781 aagctccttt gaagatgtat ccatattatt agcattttct gaaacatact ttagtacact
     9841 ggtctgttta ttaatcatgt ctacaatagc cctggacagt tcccccactt cgtcatttct
     9901 attaatcttg tatttatttg tattaatcat ataatctcca ttagatattt tagtaacatc
     9961 tttgactact tcggaaagag ctttatgaat cacggttcca aagaataagg ctgcaataat
    10021 tccaagaaca attgctatta taatcgtaag caataattta ttaagaatgg cattactcat
    10081 atcaatgacg tcttctttta gtacccagac actgaaatgc ccgattaatt cctgattttc
    10141 atctaaaatg ggtttgtaaa gcacttcata tttattgcca ttcacttcaa cttcgtccat
    10201 aacattttgc ttactttctt ctaattgctt c
//
LOCUS       DAOSUD010000069         9980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3721_length_9980_cov_17.492997, whole genome shotgun sequence.
ACCESSION   DAOSUD010000069 DAOSUD010000000
VERSION     DAOSUD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_9980_cov_17.492997"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLG49_08540"
     CDS             complement(<1..669)
                     /locus_tag="PLG49_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ17404.1"
                     /translation="MKNNIIKDSIVKHDIVKDDDIAKDGISKDDMIKDHLVEDDMAKD
                     NIVKDEFATDDRLAENIIPDQFMLPFVELDNPVSYNIPEGYVALTFDDGPSKYSKEIV
                     DILKEYGIGGTFFFIGLNVKKYPEHVKYIHSNGYSIGTHSMNHSNLPTLSNEKQEKEL
                     IQSSQLIESLTGEKLSLFRPPYGAMNQNVKDLANQYGYKIILWNNDPEDWRNKDADAI
                     VNHIK"
     gene            complement(948..1364)
                     /locus_tag="PLG49_08545"
     CDS             complement(948..1364)
                     /locus_tag="PLG49_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17405.1"
                     /translation="MSVYHGKLLELVAVKKKDEKYFLHIRISFEQDIEVYLEIDEYTF
                     INLINITNFWGRYKYRISFNTIWNETKKQHIGVLIQTYREQSEQICFPCSEEYINKLN
                     AIKNLQHIHDLEQLDFIFTHPFSSNKKDFKDIEQKI"
     gene            complement(1408..2031)
                     /locus_tag="PLG49_08550"
     CDS             complement(1408..2031)
                     /locus_tag="PLG49_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17406.1"
                     /translation="MPHEKGEKKGLTWLKNIRSSQVQTEPTTVEPQSSVELSEEIEQT
                     EPQNNTELFSKDTQDKIVLDLIVSLENLLKDRQLMLYKNKALEEQLHNANESINRLKQ
                     DQLKKEQLLQEKNKEIRSLEDILTSKQMSYEQLLEDYKEYQSMTKINDLEEKIRNLEI
                     EKQQLEQQYQKIIQEKTELLQTINDFTERMSFSFLTKNTASNSSAQE"
     gene            2344..4341
                     /locus_tag="PLG49_08555"
     CDS             2344..4341
                     /locus_tag="PLG49_08555"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOQ17407.1"
                     /translation="MRSNVLMKEPVYEPYSRFNFSSNVYSTVIVFTLILGGLYYMSIQ
                     NYLFFHSIVELICITIGAVIAIMAVNTYPFETQAGLLILGIAYGVVSIFDAIHMLSYK
                     GMSVLAGHCSTNLPVQMWVIARYIEGISIFAAILLFKKQIQLKKVLWIYYAVSAFLLF
                     STFIWDIFPECCAEGVRPTAFAVISEYIISIVLIISLIVILRKKKYFSDRIFYFFVVS
                     ICFTIVSELLISFCIDMGEVFSVFAHYFKLVSFYFLYKAFIEFDLKAPHRNLQKSEKL
                     YRILFDTIPTGVIIHFNEKIQIANNAFIHLAGEASKERVLKTPIWDYFDVEKRESFRK
                     VIEQVKTQNTDIVFEEKLMRNDGNFLDVVVNITPYTYESEPAVLITIKDITSTKQVEF
                     FKCQIEETTAYQKLQRDFFSNLSHELKTPVNLIFSTVQLLETHFKDALLENEKMKRYM
                     RILRQNCYRMLRLINNLIDVTKMDSGYFKLNLKNYNIVNVIEDIVLSTAEYMDSKGIS
                     LLFDTDVEEKIMSVDVNMIERIILNLLSNAIKFTDRGGEITVKIEDGEDYITIIVKDT
                     GIGIPKEYQKIIFDRFGQVDKMKSRNRQGSGLGLSLVQSLVELHGGTISVQSEYNQGS
                     EFIIKLPVKVLPEEEYPVILKDEEVDKYVEMMHIEFSDIYC"
     gene            4582..5259
                     /locus_tag="PLG49_08560"
     CDS             4582..5259
                     /locus_tag="PLG49_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ17408.1"
                     /translation="MKILLVEDDRTIASGLEYSLDQEGYHTVLCYDVTSAKRALMENE
                     FDLCLLDVSLPDGSGYELCKIAREKWDIPIIFLTACDDEVNVVMGLDMGADDYITKPF
                     RIRELISRIKSVLRRYQKGIKSKHILELGDIRINTLDAKVYKNGKEVNLTPLEYRLLL
                     TFANNEGQILSRSQLLEGIWDVAGDFVNDNTLTVYIKRIREKIEDDPQNPMIIKTIRG
                     LGYKVGD"
     gene            5261..6265
                     /locus_tag="PLG49_08565"
     CDS             5261..6265
                     /locus_tag="PLG49_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ17409.1"
                     /translation="MLRNREIQHFAILMFLIFAAGSTFIFILNPIAGIISVITFLLIM
                     GCSFFYTFRRYEEIKKLSNYLRMLSNGDHALDIRDNREGELSILKNEIYKVTLMLSKQ
                     GELLKKEKEQLADALSDISHQLKTPLTSMTVMTDLLSNDNLTKEKRIEFTKNIEAQLE
                     RMEWLLTSLLKLSKIDAGTVTFKKNKVLVSQLIQKAINPLLIPMEIKEQKLIVEGDEK
                     TSFTGDMNWSVEAIINIVKNCIEHTPKGGTISITFHENPLYTEIKISDNGIGIEKEDL
                     PYVFKRFYKGKNASKDSVGIGLAMAKSIISNQNGDINVTSQKNQGTEFSMKFYKNISD
                     "
     gene            6344..7027
                     /locus_tag="PLG49_08570"
     CDS             6344..7027
                     /locus_tag="PLG49_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17410.1"
                     /translation="MEILKVEHLSKIYGSGETAVKALNDVSFSVEKGEFVAIIGPSGS
                     GKSTLLHMLGGVDIPTEGKVFIDGTDIYSLDETQLAIFRRRQIGLIYQFYNLIPVLNV
                     EENITLPLLLDGHQVDSEQFRNIVKILGLENRLHHLPNQLSGGQQQRVSIGRALINNP
                     ALMLADEPTGNLDSKNSHEIIDLLKMFNKTFQQTLIIITHDERIALQADRIIAIEDGK
                     ITKNEVIRP"
     gene            7024..9636
                     /locus_tag="PLG49_08575"
     CDS             7024..9636
                     /locus_tag="PLG49_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ17411.1"
                     /translation="MNIVNKLTLRQLKLNKKRTLVTIIGSIISVAMVTAVATLGISFM
                     DLMRRQNIADQGEWHVVYNNVNKTQVEAIKNDAETKDLILERVLGYAPINSQNLNKPY
                     LFLMEYNKEGFDKFPIELLEGRFPEKEDEIVISEAILSNAKLQYKIGDVITLDIGERH
                     YTSEEGEEQILSQNEPLQKTSEGIQETLISDITKTYTIVGMISRPTWEPTWSPGYTAL
                     SYMDEKTITSENTVNAAVVLQHVNDSLFEYGEELAEKNGIEKVEFNHSLLRYYGAIKD
                     NQLRNMLFYLSAIIMIIIVIGSVSLIYNAFAISVSERSRYLGMLSSVGATKKQKRDSV
                     FFEGTVIGIISIPIGIIAGLVGIGITFICINPIIKGALGVTENFRLVVLPSSILVAVL
                     ISALTIFISTYIPARRASNISAIDAIRQVVDVKLTSKEVKTSRLTRKIFGIEGDIGLK
                     NLKRNKRRYQATVFSLIISMVLFLVVSAFTAVLKKSIVLSQDGINYDIQVSIESENEE
                     EQESIIKKINLLEHITESTHIERFEVQTWVEEEYIADYLKGEHEDIMKNDKYPYSISV
                     NVLDDESLEVYAKEVGINFKRLKDTENPGVIIIDTVKYKDTEEDKYIETKAIKTREGE
                     KLDLNYYDWENEKEIPLESVEILALTDKSPSGVFSQRIYSGFNMILSKDVYEKIKNNA
                     PIINKDAYSLLYIKSDMPLKLQEDIEAIQNNENQNNIYLYNFFLARQREEQMILLMSV
                     FTYAFIILITAICIANIFNTVSTSIALRKREFAMLKSVGMTPKSFNKMIYYESIFYGL
                     KALLYGMPISFAVMYLLHRTLMIKFSFEFFIPWTNVGIAIGAVFIIVSTAMFYSSKKV
                     KKENIIDVLKQDIA"
BASE COUNT         3344 a         1488 c         1861 g         3287 t
ORIGIN      
        1 tttaatatga tttacaatgg catctgcatc tttgttgcgc caatcttccg ggtcattatt
       61 ccaaaggatg attttatatc catactggtt tgctagatct ttgacatttt gattcatagc
      121 cccataaggc ggtctaaaaa gagataattt ctcgcctgtt aaactttcta tcagctgact
      181 agattgtata agttcctttt cttgcttttc gttcgaaaga gtaggaagat tactatgatt
      241 catcgaatga gtaccgatgg aatatccatt ggagtgaatg tacttgacat gttcaggata
      301 ctttttaaca ttaagcccaa taaagaaaaa tgtgccccca ataccgtatt cctttaagat
      361 atccacgatt tctttggaat attttgaagg accgtcatca aaggtaaggg cgacatatcc
      421 ttctggaata ttataagata cagggttgtc aagttctaca aaagggagca tgaattggtc
      481 cgggataata ttttccgcaa gcctatcgtc tgttgcaaat tcgtccttta caatattatc
      541 ctttgccata tcgtcttcca caaggtgatc ttttatcata tcatccttag agataccatc
      601 ttttgctata tcatcatctt ttacaatatc atgcttcaca atactgtctt ttataatatt
      661 atttttcaca atatcatcat ccttcacagt tctatccttt acaacattat cttgtgcagt
      721 atcctcgatg ataatttcct cttttataac agaatcctcg ttgtaagacg gctctatatc
      781 tgtttctata aatgtggctt gagtgtataa ttttctgtca ggtttaattt tacctattaa
      841 atatccttga ctagagcagc taaatagggt gataaaaaga atgctaaaaa acgtaacggt
      901 tgttcttgta tatgaaagac aatatttcct gatcggagca gcgtgggtta tattttttgt
      961 tctatatcct taaaatcttt cttattcgat gaaaaaggat gagtaaagat aaagtctagt
     1021 tgctctagat catgaatatg ttggagattc ttaatagcat ttagtttatt gatatactct
     1081 tccgaacaag gaaagcatat ttgttcactt tgttccctgt aagtttgtat taatacgcct
     1141 atatgctgtt ttttagtctc attccatatg gtgttaaaag agattctgta tttatacctt
     1201 ccccaaaaat ttgtaatatt tatcaaattt ataaatgtat attcatcaat ctccaaatat
     1261 acctctatat cctgttcaaa ggatattcta atatgtagaa aatatttttc atccttcttt
     1321 tttactgcta ctagctcaag taattttccg tgatagactg acatgattgt cccccttgta
     1381 ataggtatcc atacttttta ataatattta ttcttgagcg gatgaattag aagcagtatt
     1441 ttttgttaag aaggagaaag acattctttc agtaaagtca ttaatggttt gtaataactc
     1501 tgttttctcc tgaataatct tctgatattg ctgttctaat tgctgctttt cgatttctaa
     1561 atttctaatt ttttcttcta gatcattaat ttttgtcata ctttgatatt ctttataatc
     1621 ttcaagaagt tgttcatagc tcatttgttt gcttgtaagg atgtcttcca atgaacgaat
     1681 ttctttattc ttttcttgta aaagctgttc tttctttaac tgatcttgct ttaaacggtt
     1741 gatggattca tttgcattat gtagttgttc ttccaaagct ttatttttat aaagcattaa
     1801 ttgacggtct tttagcagat tttcaagaga aacaattaaa tctaatacga ttttatcctg
     1861 agtatctttt gagaataatt cagtgttgtt ttgtggttct gtttgctcga tttcttcaga
     1921 caattcaact gaggattgag gttccactgt ggtgggttcc gtttgaactt ggctagagcg
     1981 aatattttta agccaagtaa gtcctttttt ttcgcctttt tcatgtggca cgatatcaac
     2041 tcccatatca gtttatagca ttaaggattt ttctctatat tataacattt tgcacggttg
     2101 agaaatgttt taaatacaaa aatagcaaaa aagtcctagt ataagacaac ttttttgtca
     2161 aggtctctct atccacaaaa atatattagc tgtgggattt tctaaatcgc agatttatta
     2221 tatgataata attgacaata attatagaaa caattatcat aataattaag tatattatgt
     2281 aaaaaaatac ttaagatggt gttaataatt taacaaaatc ttataatgtt gcagggtgtg
     2341 attatgagaa gcaatgtgtt aatgaaagaa ccagtttatg aaccttacag ccgatttaat
     2401 tttagcagta acgtttacag tacagttatt gtatttaccc tcattttagg cggcctttat
     2461 tacatgagca ttcagaatta cttgttcttt cacagcattg ttgagttaat atgcattacg
     2521 attggcgctg ttattgccat tatggctgtt aatacctatc cttttgaaac tcaggcaggg
     2581 cttcttattt taggaatagc ttatggagtt gtttcaattt ttgacgcaat tcatatgctt
     2641 tcttataaag gaatgtcggt attagcagga cattgcagta cgaatttacc tgtgcagatg
     2701 tgggttattg caagatatat agaagggata tctatttttg cggccattct tttgttcaaa
     2761 aaacagatac aattaaagaa agtgttatgg atttattatg cagtgtctgc tttcttattg
     2821 ttttctacct ttatatggga tattttccct gagtgttgtg cagaaggtgt aaggcctact
     2881 gcttttgcag tgatcagtga gtatattata tcgattgtgt taattatttc acttattgtt
     2941 attcttagaa aaaagaaata tttttccgat agaatatttt atttctttgt agtatctata
     3001 tgtttcacta ttgtttctga actgttaata agtttttgta ttgatatggg tgaggtgttc
     3061 agtgtgtttg cccattattt taaactcgtt tcgttttatt ttctttataa ggcatttata
     3121 gagtttgatt taaaagctcc gcatagaaat ctgcaaaaaa gcgaaaagtt ataccgaatt
     3181 ttatttgata caattcctac gggagttatt attcacttta atgaaaaaat acagatagcc
     3241 aataacgctt ttattcattt agcaggagaa gcatccaaag aacgggtact gaaaacaccg
     3301 atctgggatt actttgatgt agaaaaaaga gaatctttcc gtaaagttat agaacaggtg
     3361 aaaacgcaaa ataccgacat agtgtttgaa gaaaaactca tgcgaaacga tggaaatttt
     3421 cttgatgtag tggttaatat tacaccttat acctatgaaa gtgaaccggc ggtattaatc
     3481 acgataaaag atattacatc aaccaaacaa gtagaatttt ttaaatgcca gatcgaagaa
     3541 acaacagctt atcaaaaact ccaaagagat ttcttttcca acttgtccca tgaactcaaa
     3601 acgcctgtca acttgatttt cagtacagtt cagcttttgg aaacacattt taaagatgct
     3661 ttattagaaa acgaaaaaat gaaacgatat atgcggattt taaggcagaa ttgctatcgt
     3721 atgctgcggc ttatcaataa cctcatcgat gtaaccaaga tggattcggg ttattttaaa
     3781 ttaaatctta agaattataa tattgttaat gtcatagaag acattgtctt atccactgca
     3841 gaatacatgg acagcaaagg catttcccta ctttttgata cggatgttga agaaaagatt
     3901 atgtctgtcg atgtgaatat gatagaaaga attatcctga atcttctttc caatgcaatc
     3961 aaatttaccg acagaggcgg ggaaataacg gttaagatag aagatggaga agactacatt
     4021 acgattattg taaaagatac aggcatcgga attccaaaag agtatcagaa aatcatattt
     4081 gacaggtttg ggcaagtgga taaaatgaaa tcaagaaaca ggcagggcag cggcttaggt
     4141 ttgtcactgg tacaatccct ggtagaattg catggaggaa ccatatctgt ccaaagtgaa
     4201 tacaatcaag gcagtgaatt tattattaaa cttcctgtta aagttcttcc tgaagaagaa
     4261 tatcccgtta tcctaaaaga tgaagaagtg gataaatatg ttgagatgat gcatattgag
     4321 ttttcagaca tatattgtta aaatttagaa gggctacact tgaatataat tatataaagc
     4381 actttctgtc aattttctgc cagaaggtgt ttttttgtgg ggaataagga ataagccgat
     4441 gtagatgtga gttttgcggt attagcaatc aagttcaaaa cagattgcac catgaaaaag
     4501 ataggttata atattaatga aataaattag catgagaaaa gtaacgtata aacagttgac
     4561 acactatttg gaggtgagtg cttgaagatt ttactcgttg aagatgatcg aacaattgcc
     4621 agtggcttag aatactcctt ggaccaagaa ggctatcata cagttctttg ctatgatgtt
     4681 acatctgcaa aaagggcttt aatggagaat gaatttgatt tatgtctcct tgatgtatct
     4741 ctgcccgatg gaagtgggta tgaattatgt aagatagcaa gggaaaaatg ggatattccc
     4801 attatatttt tgacggcttg tgatgatgaa gtcaatgtgg tgatgggact tgatatggga
     4861 gcagatgatt atatcacaaa gccctttcgt atcagagaac ttatttctcg tatcaaatca
     4921 gtgctcagaa gatatcaaaa gggcataaaa tcaaaacata ttctcgaact gggggatata
     4981 cggattaata ctcttgatgc aaaggtatat aaaaatggaa aggaagtaaa tcttacgcct
     5041 ttagaatatc gcctgctatt aacttttgct aataatgaag gacaaattct gtcacgaagt
     5101 cagctcttag aagggatttg ggatgttgcc ggtgattttg ttaatgataa tactttaact
     5161 gtgtatataa agcggattcg agaaaagata gaagatgatc ctcaaaatcc aatgattatc
     5221 aagacaattc gtggccttgg atataaggtg ggtgattgat atgcttcgca atagagaaat
     5281 tcaacatttt gcaatcctta tgttccttat attcgctgct ggaagtactt ttatttttat
     5341 tctaaaccca attgctggaa tcatatccgt gataactttt ttactcatta tgggatgtag
     5401 ttttttctat acgtttagga gatatgaaga aataaaaaag ctctctaatt atttaaggat
     5461 gctttccaat ggggatcacg cattggatat tcgtgataac agggagggag agcttagtat
     5521 tcttaaaaat gaaatataca aggtaaccct tatgctctcg aaacaaggag aacttcttaa
     5581 aaaggaaaag gagcaattag ccgatgccct ctctgatatt tctcatcagc ttaaaactcc
     5641 gctgacctct atgactgtta tgacagattt attaagtaat gacaacttaa caaaagaaaa
     5701 aagaattgaa tttactaaaa atattgaagc gcagctggag cggatggaat ggcttttaac
     5761 ttctttactg aaactctcta aaattgatgc tggaacagta acctttaaaa agaataaagt
     5821 attggtctct cagctgattc agaaagcaat caatcctctc ttaattccaa tggaaattaa
     5881 agaacaaaag ctcattgtcg aaggtgatga gaagacatct tttacaggag atatgaattg
     5941 gagcgttgag gcaatcatta atattgtaaa aaactgtata gagcatacgc ctaaaggggg
     6001 gacaatctct atcacattcc atgaaaatcc tttgtatacg gaaattaaaa tttcagataa
     6061 tggaattggt atagagaagg aagacctccc ttatgttttt aaacgattct ataaaggaaa
     6121 gaatgccagc aaagacagtg taggcatagg tcttgccatg gcaaaaagta taataagcaa
     6181 tcaaaatgga gatatcaatg ttacaagtca gaaaaatcaa ggtactgaat ttagcatgaa
     6241 attttataaa aatataagtg actaaattgt aatattaaag tcactgcaaa gtcatctaag
     6301 gcagatatac taaagtcaca ataagaattc tggaggttgt agcatggaaa tattaaaagt
     6361 tgaacatctt tctaaaattt atggtagtgg cgaaacagca gttaaagcct taaatgacgt
     6421 ttctttctca gtagaaaaag gagaatttgt tgccattata ggaccgtctg gttccgggaa
     6481 atccacttta ttacacatgc ttggaggtgt ggatatacct actgagggta aagtgtttat
     6541 agatggcaca gatatataca gtttggatga aactcagctg gcgattttta ggcgcagaca
     6601 gattggtctt atctatcaat tttataatct tatccctgtg cttaatgtgg aagaaaacat
     6661 caccttgcct ttgctgcttg acggacatca ggtcgattct gagcagtttc gaaatatagt
     6721 aaaaatactt ggtctggaaa acagactcca tcatttgccc aatcaacttt ccggcggtca
     6781 gcagcaaaga gtatccatag gaagagccct tattaataat cctgctttga tgctggcaga
     6841 tgagcccaca ggaaatcttg acagtaaaaa tagtcatgag attattgatc ttttaaaaat
     6901 gttcaataag acttttcagc aaacgcttat tatcattacc catgatgaac ggattgcatt
     6961 acaggctgat agaatcatag ctatagaaga tgggaagatt accaaaaacg aggtgattcg
     7021 cccatgaata tagttaataa gttaacttta agacaactca aattaaataa aaaaagaacc
     7081 ctcgttacca ttatagggtc cattatctca gtagcaatgg ttacagcagt agctacctta
     7141 ggaatttcat ttatggattt gatgaggaga caaaacatag ccgatcaagg agaatggcat
     7201 gttgtttata ataatgtgaa taaaacacaa gtggaagcga ttaaaaatga tgccgaaaca
     7261 aaagatttga ttttagagag ggttttaggc tatgccccca taaatagcca aaacttgaac
     7321 aagccctatt tatttcttat ggaatataac aaagaaggtt ttgataaatt tccaatagaa
     7381 cttttggaag gaagatttcc agaaaaagaa gatgaaatcg tcatctctga agccatatta
     7441 tccaatgcaa aacttcagta taagatagga gatgtcatta ctcttgatat tggagagaga
     7501 cattatacca gtgaagaagg agaagagcaa attctatcgc agaacgagcc tttacaaaaa
     7561 acttcagagg gaattcaaga aacattaata agcgatatta caaagactta taccattgta
     7621 ggcatgatca gtaggcccac ttgggaacct acctggtctc cgggatatac ggcattatct
     7681 tatatggatg aaaaaacgat aacatctgag aacactgtaa atgctgcagt tgttttacag
     7741 catgttaatg attcactatt tgaatatgga gaagagttgg cagaaaaaaa tggcatagag
     7801 aaagtagaat ttaatcattc tttattaaga tactacggag cgataaaaga caatcaatta
     7861 aggaatatgc ttttttattt gtctgccatt attatgatca ttattgtgat tggctctgtt
     7921 tcattaatat acaatgcttt tgcaatttca gtttcggaac gatcgagata tctaggtatg
     7981 ttatccagtg taggtgctac aaaaaaacaa aaacgagatt ctgtattctt tgaagggact
     8041 gtgattggaa taatcagtat tcccattggg attattgcag gccttgtagg aatcggaatt
     8101 acattcattt gtattaatcc gattatcaaa ggcgctctgg gtgtaactga aaatttcagg
     8161 ttggtcgtat tgccttcttc tattttagtt gcggtactta tatctgccct tactatcttt
     8221 atttcaactt atatccctgc ccgaagagct tcgaacatat ctgccataga tgctattcgt
     8281 caagtagtag atgtaaaatt gaccagtaaa gaggtaaaaa catcaagatt aactcgaaag
     8341 atttttggga ttgagggaga tataggcctt aagaacttaa aaagaaacaa aagaagatac
     8401 caggcaacag ttttttcgct gatcattagc atggttttat ttttagtagt gtccgctttt
     8461 acagcagtgt taaagaaatc catcgtgctt agtcaagacg gaataaacta tgatattcaa
     8521 gtgtcgattg aaagtgagaa tgaagaagaa caggaatcta ttattaaaaa aataaatttg
     8581 ttggagcata taacggagtc aacccatatt gagcgtttcg aggtacaaac ttgggtagaa
     8641 gaagaatata tagcggatta tttaaaaggt gagcatgaag acataatgaa aaatgacaag
     8701 tacccttatt ctatatctgt taatgtattg gatgatgaat ctttagaagt ttatgcgaaa
     8761 gaggtaggaa taaactttaa gcgcctaaaa gacacagaga atcctggggt aatcattatt
     8821 gatacagtaa aatataaaga cacggaagaa gacaaatata ttgaaactaa agcgattaaa
     8881 acccgtgagg gagaaaaact ggatctaaac tattatgatt gggaaaatga gaaggagata
     8941 cctttagaat ctgtagaaat ccttgcgctt acagataaat cgcctagcgg tgttttctcc
     9001 caaagaattt attcaggatt caatatgatt ttatctaaag atgtctatga aaagattaaa
     9061 aataatgctc cgataatcaa taaagatgcc tacagcttgc tctatataaa aagtgatatg
     9121 cctctaaagc ttcaggaaga cattgaagct atacaaaata atgaaaatca aaataatatt
     9181 tatttgtata atttctttct ggccagacaa agagaagaac aaatgattct tttaatgtcg
     9241 gtgttcacgt atgcgtttat tatactcatc actgccattt gtattgccaa tatttttaat
     9301 accgtatcga ccagtattgc acttaggaaa agagaatttg caatgctcaa atctgttggt
     9361 atgacgccga aaagttttaa taaaatgatt tattatgaaa gtatttttta tggtttaaaa
     9421 gctctgttat acggaatgcc cataagtttt gccgttatgt acctattgca ccgaacattg
     9481 atgataaaat ttagttttga attttttatt ccatggacca atgtaggtat agccatagga
     9541 gctgtgttta tcatagtaag tacggcgatg ttttattcca gcaaaaaagt aaaaaaagaa
     9601 aatattatag atgtgttaaa gcaagatatc gcttaataca aagtacactt atcctaatct
     9661 atttagagca aaactctatt tggattagga tttttttaag aaatttaaat gtgcaacata
     9721 taataataat aaagattctt gacgtgctta cataaaagct tttatgaatg tggaatactt
     9781 aaagtaaaat gatattttaa gcagaaagga agaggtgctg tgcaaagatc tagaacagga
     9841 tggattgtgt tagggattgt attaatcgtt ttactgatta tagggtctag tgtcattggc
     9901 agttataata gtttggcagc agccagtgaa aatattgaca gcaaatggag tcaggtagaa
     9961 aacaatcttc aaagaagggc
//
LOCUS       DAOSUD010000070         9948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3733_length_9948_cov_17.706965, whole genome shotgun sequence.
ACCESSION   DAOSUD010000070 DAOSUD010000000
VERSION     DAOSUD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3733_length_9948_cov_17.706965"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLG49_08580"
     CDS             complement(<1..254)
                     /locus_tag="PLG49_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17412.1"
                     /translation="MRIAFFVNKFPSTSETFILNQITGLLDKGHTVDIYAHYRNRKTK
                     IHPVIKEYKLLNKCHYLCWEVNDPWYKKIEMFKLAFFNTI"
     gene            complement(285..1559)
                     /locus_tag="PLG49_08585"
     CDS             complement(285..1559)
                     /locus_tag="PLG49_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOQ17413.1"
                     /translation="MKTVQDLDLTYVCTGCGTCEVVCPANAIKIKTKEGQYFPEIDMG
                     KCIHCSRCIHICPGREENNRTSLKTCFPNSIYDKSIGYYQATYIGYSIDKEIRFKAAS
                     GGITTTVLLYLLENQWIDGAIVTKQDPKNPLKNLSFVARTKEDILESVGSKYSPTHTV
                     SAIKEIKDKPGQYAFVGLPCQIRAIRKLQEKYSWVKEKIVLTIGLFCSRGVTYKGTEF
                     VVDKFARGTRKIKSIKYRGDGWPGGCRISYANKKYTIALKDYWDPYFANYFFTPYRCL
                     VCGDLFSEGADISLGDAWLPEIKKKDSIGTSIIISRSKIAEEILLKLKEENILHLNIC
                     PKKAVIISQKGIIKRKINALYARRILFKLLRRPVPKEDKDNKDSYAVKDFIMAAMMLF
                     NAAFSKSRLGQKILLRVPKDVLLKYRNILHKL"
     gene            complement(1583..2656)
                     /locus_tag="PLG49_08590"
     CDS             complement(1583..2656)
                     /locus_tag="PLG49_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOQ17414.1"
                     /translation="MDSKLAVDKYKRLVKKICLYLKIVYKNIVFIYKNREYKNKKKII
                     YLLTPPPRLSNVGDHAQAVAIHKWFNKHFSHLPVLEFNKDDSDSYFLALKYLVNKDDI
                     IFLHSGGNLGDRGIWSESIRRKYIKGFRNNKIISLPQTIYFSSTKKGRAEKKKTKEIY
                     NRHNDLIIMGRDEESSNIARHMFTKAKIYNVPDFVLSLDKEYKPPTEKKTCQKILLCL
                     REDKESILSEKEKRDLVQSLPYDCRRYDTTLKKPIYKRERTLENTLKMFNKYDLVITD
                     RYHGLIFSVLCKKPCIVIPTVDHKLTSAINWFKDIPTVSLIKDLNDIPKEIKKITANN
                     QNIVIDWNAKYFDPLAEQLKKEL"
     gene            complement(2700..3647)
                     /locus_tag="PLG49_08595"
     CDS             complement(2700..3647)
                     /locus_tag="PLG49_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17415.1"
                     /translation="MKALESEIGRIIGSLREKGSVIKEFNFIEDIDKEKSNRSRYDAN
                     DSFMEFHYALSMDARLIHKETVLGDCIEEAGKITLIPTLNNEYKDQIKRAGLIFNSSI
                     DPSIDMLAVKFVLTNELVNFYCILANDSFYHYITDKSPEKIMEHPADSYQLACCFNNK
                     GCTVKERKGKNYISTSPVFIDMHEGENRYLLDLIRQKIKINNEKFRNFIKTENTQANY
                     LYLAAVGEGTVTINEMEFGETKGYIYLKPVEVNASSVMLNMVYTGEVEVEYFNDEGKW
                     EHISINENITSGSRLLHIRLLMETGSKVFYLNISKGKDS"
     gene            complement(3680..5005)
                     /locus_tag="PLG49_08600"
     CDS             complement(3680..5005)
                     /locus_tag="PLG49_08600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOQ17416.1"
                     /translation="MLHRIGIIGSGYVGLVTGACLADFGMKVICVDVDEEKINQLKEG
                     KIPIYKPGLEQLVERNTYYKRLSFTIDLKEAVHNSDVIFIAVGTPPAEDGSADIAHVL
                     KAAEDIAYHMNEYKVIVDKSTVPVGTGQKVKKTIQSVLKKRNVQIDFDVVSNPEFLRE
                     GSAIYDFTHPDRVVIGGESQKAIEIMKDVYDVLYLNETPFIITNIETAEMIKYASNGF
                     LAMKITYINEIANLCEKVGANVQEVARAMGRDGRIGPKFLHPGPGYGGSCFPKDTRAL
                     ASIARAHHSPVTLIEATIEANERQKLIMVKKVTDALGDLNNKTLAILGVTFKQNTDDM
                     RESPALTIIGELEKLGAKFRIYDPQGMKEAKWRLKAIEEKCVYCKDEYDAVKDADALL
                     IITEWNQFRNLDIDTIKSSMKGNYFFDFRNIYDREYIENKGFNYYAVGR"
     gene            complement(5143..6135)
                     /locus_tag="PLG49_08605"
     CDS             complement(5143..6135)
                     /locus_tag="PLG49_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ17417.1"
                     /translation="MFQGKKILVIGGTGTIGQGLVKELIKRNPKVIRIFSRDEYKQFK
                     MEQELGRLENIRFLLGDVRDKERVQRAMEGIDIVFHLAALKHVPSCEYNPFEAIKTNV
                     MGTQNVIESAIHHRVERVVYTSSDKAINPTNTMGATKLLAERLMSSADYYKGGKRPIF
                     ASVRFGNVMGSRGSVIPLFKTQILEKRKVTVTDPAMSRFMMSITEAVSLVLKAAEYAV
                     GGDVFVLKMPVVNIKDLVEVVIEETCKKYDLPREEIKIEYIGLRPGEKMYEELMTKEE
                     GERAEELEEMFRIYPITRAGINITGINGYSTDSSKVKALNKEEIRNLILREDLI"
     gene            complement(6205..7620)
                     /locus_tag="PLG49_08610"
     CDS             complement(6205..7620)
                     /locus_tag="PLG49_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ17418.1"
                     /translation="MIHYEKNILLLKQKDLLLYNKMNLYQSDKEKYIPVMTTSGDMTL
                     KVISYDYTRQKQIDFFLHSKYNPIKEAEKFAKAKYNSNKGIILYGFALGYHIEALMEL
                     LSDEQELYIFEMNLDVFKSALEIRDLSKILSHPGLHFIITDNTSDFALKLSRLLEEHK
                     NLIIYPPSLKTIPEEGGNIRFILEDWDIKKTESNEWKERVIYNYERNKRLNCPNVGEL
                     FGKYKDLPFVVVSAGPSLEKNVHLLKKLEGRAFIFAVGRVLKILLWKDIEPDMFCIID
                     PQYGPTYSQIEGYEDLDIPLVFHEYAAADTIAKYRGPKYIASDKKEHIENPGHLMELG
                     GSVATAVVDLAIRFGGNPIVFVGQDLAFTNGQSHAYGGLVNQDVSRRKVKGQNGEFLD
                     TTLGLLSFKHWIENKIRDNPRVEFINATEGGAYIEGCKHMSLQDFIDQYIDFSSPIDK
                     RLRQRKLEEEIKLFTASIEYI"
     gene            complement(7617..7979)
                     /locus_tag="PLG49_08615"
     CDS             complement(7617..7979)
                     /locus_tag="PLG49_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17419.1"
                     /translation="MDKREEKMELVRTAADYTGRLIEGINEAVDLFHEGMEGKALDLV
                     IQIIDGLKWTMEAITVTLDVQEEAIDISEINDAFNAMSEALENVDYVLIADVLEYEIL
                     PKVEEWNQKLFITAGEES"
     gene            complement(7997..9853)
                     /locus_tag="PLG49_08620"
     CDS             complement(7997..9853)
                     /locus_tag="PLG49_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ17420.1"
                     /translation="MSSSLFEKNVSQYCKKYGKIIKNFTDTSNLLLEKTKDDCYTLKL
                     KYLDRELYLHSKYSPIKEAERFVEKIKDFNIKTVFIIYGMGLGYHILELMKYLHEENS
                     LIIIEPNEEVLSKAFSVFDWTKLLNRKKTHLFTGTDRQAINLLLGEFVDITNAENIQF
                     LTFSQYDKIYPEFYKVVYEEIKNYVSLFKISKNTKTHFWNKYNENFFNNLTKMVHEHS
                     INDLKGQFNNVPAFIVSAGPSLDKNIKALRQVQNRGLIFSGGRTLTALIHNNIRPHFI
                     VSMDPGIAAYEVLQEHKKHDIPLITTVVSNDEVIQAHQGDQFYIANSEYTGLIHYFTK
                     KEMDILPLGASVANASAAIALYLGCNPIIFVGQDLAYTDGKYHAQSCQIGQEETDEDD
                     RFEVEGIYGGKVWTNSVWYTFLMWMEKFIYHSSGKLFIDATEGGAKIHGTEILTLKES
                     IEKYCQKTFDVDEKLKEIISNHEKVYQNEKLKTLLNNLKNNLDKLDKISDKALKDLEE
                     VYHCDIESITIEHTRPFRVFEEQINKMMKDNPGIKDILIRVFEEINSNNDFIEKVNET
                     EKEKLEKIIKRNQEFYRQIKITVKSIEGFIENLIENQLESIKIFGARIHFKS"
BASE COUNT         2975 a         1952 c         1313 g         3708 t
ORIGIN      
        1 tttattgtat tgaaaaaagc aagtttaaac atttctattt ttttatacca gggatcattt
       61 acttcccaac ataaataatg acacttattt aataatttat actcctttat aactggatga
      121 atttttgtct tcctgttacg ataatgagca taaatatcta ctgtatgccc cttatccaac
      181 aaacctgtaa tttgatttaa aataaatgtc tctgatgttg aaggaaattt attcacaaaa
      241 aaagctatcc gcattttacc tctcctaata attgtatttt ttcatcacaa tttgtgcagt
      301 atatttctat actttagcaa tacatccttt ggtactctca aaagtatttt ttgtcctaat
      361 ctacttttgc tgaaagcagc attaaaaagc atcatagcag ccataataaa atctttaaca
      421 gcataagaat ccttattgtc tttatcctct ttcggaactg gtcttctcaa taatttaaac
      481 agtattcttc ttgcatacaa agcattaatc tttcttttga taataccttt ttgcgatata
      541 ataactgctt tcttaggaca aatatttaaa tgaagaatat tttcttcctt taattttaat
      601 aagatctctt ctgcaatctt tgaccttgaa ataataatag aagtaccaat actgtctttc
      661 tttttaattt ccggcaacca agcatctcct aaagaaatat ctgctccttc actaaataaa
      721 tccccacata ctaaacaacg ataaggtgta aaaaagtaat tagcaaaata gggatcccaa
      781 taatctttaa gagctattgt atatttttta ttagcatagg atattctgca gcctccaggc
      841 catccatctc cgcgatattt aatcgatttt atcttccttg ttcctctggc aaatttatcc
      901 actacaaatt cagttccttt ataagtaaca cccctggaac aaaaaagacc tattgtaaga
      961 acaatttttt cttttaccca agaatacttt tcctgcaact ttctaatcgc acgaatctga
     1021 caaggtaacc ctacaaaggc atattgacct ggtttatctt taatttcttt aattgccgaa
     1081 accgtatgag taggagaata tttacttcca acactttcaa gaatatcttc ttttgttctt
     1141 gctacaaaag aaagattttt taatggattt tttggatctt gtttggtaac aatcgctcca
     1201 tcaatccatt gattttctaa taaatataaa agaactgtcg tcgtaattcc ccctgaagcc
     1261 gctttaaatc taatctcctt atcaatagaa taccctatat aagttgcctg ataataacct
     1321 atacttttat cataaataga gttggggaaa caggttttta ggcttgtccg attattttct
     1381 tcccttcccg ggcatatatg aatacatctc gaacaatgaa tacattttcc catatctatt
     1441 tcaggaaaat attgcccttc tttagtttta atctttatgg cattggcagg acatacaact
     1501 tcacatgtcc cacaccccgt acaaacataa gttaagtcta aatcttgaac tgttttcatg
     1561 tttatattcc ttctttaata agttataatt ctttttttaa ttgttctgct aaaggatcaa
     1621 aatatttggc attccaatcg ataacgatat tttgattgtt agctgttatt tttttgattt
     1681 cttttggtat atcatttaaa tcctttatta atgaaaccgt aggtatatcc ttaaaccaat
     1741 taatcgctga agttaattta tgatctactg tcggaatcac gatacagggc tttttacata
     1801 gcacagaaaa aattaagcca tggtaacggt ctgtaatcac aagatcatat ttattgaaca
     1861 ttttaagtgt attttccaga gttctctctc ttttataaat aggtttcttt aaggtcgtat
     1921 catatcgcct gcaatcatag ggaagacttt gaacaagatc tcttttttct ttttcagata
     1981 aaatggattc tttatcctcc ctaagacaca ataggatttt ttgacaggtc tttttctctg
     2041 tagggggctt gtattcttta tctaatgaca agacaaaatc agggacattg tatatctttg
     2101 ccttcgtaaa catatgtctt gcaatattgc tgctttcttc atcccgtccc ataataatta
     2161 aatcattatg ccgattatag atttcttttg tcttcttttt ttctgctctt ccttttttcg
     2221 tggaactaaa ataaatggtt tgaggtaaag agataatttt attattccta aagcctttta
     2281 tatatttcct tcgaatagac tctgaccaga ttccccggtc tcccagatta cctccactat
     2341 gaagaaagat aatatcgtct ttattcacta aatattttaa agctaaaaag tatgaatctg
     2401 aatcgtcctt attaaattct aaaactggta aatgagaaaa atgtttatta aaccatttat
     2461 gaattgctac agcttgtgca tggtctccca catttgaaag cctgggagga ggcgtaagaa
     2521 gataaatgat ttttttctta tttttatatt ctctgttttt atatataaaa acgatatttt
     2581 tatatacaat ctttaaatat aagcatatct tttttaccag tcgcttatat ttatctactg
     2641 ccagtttact gtccataaat aacccccatt aataaaagga taaatagtcc agacaaatgc
     2701 tatgaatctt ttccttttga aatatttaga taaaatactt tactgccggt ttccattaac
     2761 aatctgatat gcaataatct tgatccagaa gttatatttt catttatact gatatgttcc
     2821 cattttcctt catcattaaa atattcaact tcaacttctc cagtataaac catattcagc
     2881 attactgaag aagcattcac ttcaaccggt tttaaataga tatagccttt tgtctctcca
     2941 aattccattt cattaattgt aactgttcct tctccgacag cagccagata tagataattt
     3001 gcctgagtat tttctgtttt aataaaattc ctgaatttct cattgtttat ttttatcttt
     3061 tgtcttatta aatcaagtaa atatctgttt tctccttcat gcatatcaat aaaaaccgga
     3121 ctggtactga tataattttt acctttcctt tcttttacgg tacagccttt attattaaaa
     3181 cagcaggcta actgataact atctgcagga tgctccatta ttttttcagg gctcttgtct
     3241 gtgatataat gataaaaaga atcattggct aaaatacaat aaaaatttac taattcatta
     3301 gtcaagacaa actttactgc cagcatatct atagatggat caatagaaga attaaagatc
     3361 aaaccagctc tttttatctg gtctttatat tcattgttca aagtaggaat aagcgtgatt
     3421 ttacctgcct cttcaatgca atctcctaat acagtttctt tatggataag tctggcatcc
     3481 atagaaagag cgtaatgaaa ctccataaag gaatcattag catcatacct tgaacgatta
     3541 cttttttcct tatcgatgtc ttctataaaa ttaaactctt taataacaga gcctttttct
     3601 cttaaactcc ctattattct gccaatttca ctctccaaag ctttcatttt tttgcaccta
     3661 cttttaaaat aatttctgct tatctgccca cagcataata attaaaccct ttattttcaa
     3721 tatattctct gtcatatata tttctaaaat caaagaaata attgcccttc attgaacttt
     3781 ttattgtatc aatatccaaa tttctaaatt gattccattc tgttatgata agcagtgcat
     3841 ccgcatcctt tactgcatca tattcatctt tacaataaac acatttttct tcaatggctt
     3901 taagcctcca ctttgcttct ttcatcccct gtggatcata aatcctgaat ttagccccaa
     3961 gcttttccag ttcccctatt atggtaaggg caggagactc tctcatatca tctgtatttt
     4021 gtttaaaggt tacacctaat atcgccaggg tcttattatt taaatctcct aatgcatctg
     4081 tcactttttt aaccattatt aacttttgtc tttcattggc ctcaatcgtt gcttcaatca
     4141 aagtgacagg agaatgatgc gctctggcta tacttgctaa tgcccttgta tcttttggaa
     4201 aacaacttcc tccataacca ggacccgggt gaagaaattt cggacctatc cttccgtccc
     4261 ttcccatggc tcttgcaacc tcctgcacat tggcacctac cttttcacaa aggttggcaa
     4321 tctcattgat ataagttatt ttcattgcca aaaatccatt ggaagcatat tttatcattt
     4381 ctgctgtttc tatattcgtt attataaaag gtgtttcatt taaataaaga acatcataaa
     4441 cgtctttcat gatttctatg gccttttggg attctccgcc aataaccacc cggtcaggat
     4501 gggtaaaatc ataaattgcc gaaccttctc ttagaaattc gggatttgac accacatcaa
     4561 aatcaatttg tacatttctt ttttttaaca cactttgaat agtctttttt actttttgcc
     4621 cagtcccaac gggtacggtt gatttatcta caatcacttt atattcattc atatggtatg
     4681 caatatcttc tgccgctttt aaaacatgtg caatgtctgc actgccatct tcggcaggtg
     4741 gtgttcctac ggcaataaaa ataacatcac tattatgaac agcttcttta agatcgattg
     4801 taaagcttaa tcttttatag taagtatttc tttctaccaa ttgctcaaga ccaggtttat
     4861 aaattggaat ctttccttct tttaattgat ttattttttc ttcatctaca tcaacacaaa
     4921 taaccttcat accaaaatct gcaagacatg cacctgttac aagacctaca tatcctgagc
     4981 caataatccc tattctatgc aaaattatca cctgctttat aataaattct atttataata
     5041 tcgtcaaaag ctaacacaat ccttagtata attatttgta tcttttgcct aataaataag
     5101 gctgtgcact taggttaaat aacccttagt acacagccct ttttaaatta ggtcttctct
     5161 gagaataaga tttctaattt cttctttatt taaagcttta actttggatg agtctgttga
     5221 atatccatta ataccggtta tattgatgcc tgcccttgta ataggataaa ttctgaacat
     5281 ttcttctaat tcttctgccc gttctccttc ttcttttgtc atcaactctt catacatctt
     5341 ttctcctggt cttaaaccaa tatactctat tttgatttct tcccggggca aatcgtattt
     5401 tttgcatgtt tcttcgataa ccacttcaac taaatctttt atattaacta ctggcatttt
     5461 taatacaaaa acgtctcccc ctacggcata ttctgcagcc tttaatacca aactcacagc
     5521 ttcagtaatg ctcatcataa aacgggacat agcaggatct gttacagtga cctttctttt
     5581 ttcgagaatt tgtgttttaa ataaagggat cactgagccc ctggaaccca tgacatttcc
     5641 aaagcggact gaagcaaaaa taggtctttt acctccttta taataatccg cagaagacat
     5701 caaacgttct gccagaagct tcgtagcccc catggtgttg gtcggattga tagccttgtc
     5761 gctgcttgta taaacaaccc tctcgaccct gtgatgaata gccgattcta tgacattttg
     5821 cgttcccatc acattggttt ttattgcttc aaaaggattg tattcacacg aaggaacatg
     5881 tttcaatgca gctaaatgga agacaatatc aatcccttcc atagcccttt ggactctttc
     5941 cttatctctt acgtctccta ataaaaacct tatattttct agtctgccca gctcttgctc
     6001 cattttaaac tgtttatatt catccctgct aaagatacgg ataacctttg gattacgttt
     6061 tatcaattct tttaccagac cttgtccaat ggttcccgtt cccccaataa ccaaaatctt
     6121 tttcccctga aacatctttt cacctactta atttatgatc taattaaaat gctgctttat
     6181 aaaaagatct ttatcagtaa tgttttaaat gtactcgata gatgcagtaa aaagctttat
     6241 ttcttcttcc agctttcttt gccttaatct tttatctata ggacttgaaa aatctatata
     6301 ctggtcaatg aaatcttgaa gactcatatg tttacaccct tcgatatatg ccccgccttc
     6361 tgtagcattg ataaactcaa cccggggatt atctctgatt ttattttcta tccaatgttt
     6421 aaaacttaaa agccctaaag ttgtatcaag aaattcccca ttttgacctt ttacttttct
     6481 cctgctgaca tcttgattga ctaatcctcc gtaagcatga ctttgaccat tcgtaaaagc
     6541 taaatcttgt cctacaaaca caataggatt tcctccaaag cggatagcca aatccacgac
     6601 tgccgtcgca acagaaccac ctaattccat gagatgccca ggattttcaa tatgctcttt
     6661 tttgtctgat gcaatgtatt taggacctct gtattttgct atggtgtcag cagcagcata
     6721 ttcatgaaag accaggggga tatctaagtc ttcataacct tcaatttgtg aataagtagg
     6781 tccatattgc ggatcaatga tacaaaacat atcaggttca atatctttcc aaaggagaat
     6841 ttttaatact cttccaacgg caaaaataaa agctcttcct tctaattttt tgagcaaatg
     6901 cacgtttttt tctaatgaag ggcctgctga tacaaccacg aagggcaggt ctttgtattt
     6961 gccaaacaat tctcccacat tgggacaatt aagcctttta tttctttcat aattgtatat
     7021 gactctttct ttccattcat tggattcagt ttttttaata tcccagtctt ccaggataaa
     7081 acggatattt ccgccctctt caggaattgt ttttaaagat ggcggataga taattaagtt
     7141 tttatgttcc tctaacaatc gagacaactt taaggcaaaa tcacttgtat tatccgttat
     7201 gataaaatgc agccccgggt gggataaaat tttagacagg tccctgattt ctaaggctga
     7261 tttaaataca tctaaattca tttcaaaaat atagagctct tgctcatcgg ataacaattc
     7321 cattaaagct tcaatatgat atcctaatgc aaaaccataa agtataatcc ctttattgga
     7381 attatacttt gcctttgcaa atttttcggc ttcctttatc ggattatatt tactgtgcag
     7441 aaaaaaatcg atttgttttt gccgggtata atcataactg attaccttaa gcgtcatgtc
     7501 tcctgaagtt gtcatgacag ggatatattt ttctttatcg ctctgataca aattcatctt
     7561 gttatataat aatagatctt tttgcttgag taaaagtatg ttcttttcgt aatgaatcat
     7621 gactcttccc ctgcagtaat aaataatttt tgattccatt cttctacttt tggaagaatt
     7681 tcatattcca gtacgtctgc aatgagtaca taatccacat tttccaaggc ttctgacata
     7741 gcattaaaag cgtcattgat ttcagatata tcaatggctt cttcctggac atctaaagta
     7801 accgtaatgg cctccatagt ccactttaag ccatcaataa tttgaataac caaatcaagt
     7861 gcctttcctt ccattccttc atgaaataaa tccactgctt cattaattcc ttcaattagt
     7921 ctgccggtat aatcggctgc tgtacgaact aattccatct tttcttctct tttatccatc
     7981 tttattcctc ctgcttttaa gatttaaaat gaattctggc cccgaatatt tttatgcttt
     8041 ccaattggtt ttctattaag ttttctatga agccttcaat gctttttacg gttattttaa
     8101 tttgtctgta gaattcctgg tttcttttaa tgattttttc tagtttttct ttttctgttt
     8161 cattaacctt ctctataaaa tcattatttg aattaatctc ttcaaaaacc cgtattaata
     8221 tatcctttat ccctggatta tctttcatca ttttattaat ttgttcttca aaaaccctaa
     8281 agggtcgggt atgttctatt gtaatactct ctatatcaca atgatatact tcttctaaat
     8341 cttttaaagc cttatctgat attttatcaa gtttatctaa attatttttt aaattattta
     8401 ataaagtctt taatttttcg ttttgatata ctttttcatg attagatata atttctttta
     8461 gtttttcatc aacatcaaag gtcttttggc agtatttctc tatgctttcc tttaaggtca
     8521 aaatttctgt cccatgaatt ttggccccgc cttctgtggc atcaataaaa agtttgccag
     8581 aagaatgata tataaatttc tccatccaca tcaaaaaggt ataccagaca ctattggtcc
     8641 atactttgcc gccatagata ccttccactt caaatctgtc atcttcatcc gtttcttcct
     8701 gtccaatctg gcagctttgg gcatgatatt taccatcggt ataagcaaga tcttgtccta
     8761 caaatattat gggattgcag ccaagataaa gtgctatggc ggctgaggca ttcgccaccg
     8821 atgcgcctaa aggcaaaata tccatctctt tctttgtaaa ataatgaata agacccgtat
     8881 attcgctgtt tgcaatgtaa aattgatccc cctgatgagc ctgtatcacc tcatcatttg
     8941 aaaccactgt cgtgataaga ggaatatcat gttttttatg ttcctgtaaa acctcatagg
     9001 ctgcaatgcc aggatccatg gatacgataa aatgaggtct gatattgtta tgaataaggg
     9061 ctgtcaaggt tctgcctcct gaaaagataa gccccctgtt ctgtacttgt ctcaatgcct
     9121 taatattttt atccaaagaa gggcctgcag atacaataaa agcaggtaca ttattaaact
     9181 gcccttttaa atcattaata gaatgttcat gtaccatttt agtcagattg ttaaaaaagt
     9241 tttcattata tttattccaa aaatgcgttt tagtgttttt tgaaatttta aataaggaaa
     9301 cataattttt aatttcctca taaacgactt tataaaattc aggataaatt ttatcatatt
     9361 gggaaaaagt taaaaattga atgttctctg catttgtaat atcaacaaat tctcctaaaa
     9421 gaagattaat agcctgtctg tcggtccctg taaaaagatg ggttttcttt ctatttaaca
     9481 attttgtcca gtcaaaaaca gaaaaagcct tagataaaac ttcttcatta ggttcaataa
     9541 taatcaagga attttcctca tgcaagtatt tcataagctc caggatatga tagcccaaac
     9601 ccattccata tataataaag acagttttta tattaaaatc tttaattttc tctacaaatc
     9661 tttctgcttc tttgattggg gaatacttac tatgaagata aagctccctg tctagatatt
     9721 ttaatttaag agtataacag tcatctttgg ttttttctaa aagcagattt gaagtatctg
     9781 tgaaattttt tatgatttta ccgtactttt tacaatactg gctgacgttt ttttcaaaaa
     9841 gagaagaact cattattaag ctcctttatg tttgatattt ttcttaattt taattggttc
     9901 ttctctttgt ttatcgacaa aaaatcatat attatttata tcaaaaaa
//
LOCUS       DAOSUD010000071         9947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3735_length_9947_cov_24.650627, whole genome shotgun sequence.
ACCESSION   DAOSUD010000071 DAOSUD010000000
VERSION     DAOSUD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_9947_cov_24.650627"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /gene="rlmD"
                     /locus_tag="PLG49_08625"
     CDS             complement(<1..1335)
                     /gene="rlmD"
                     /locus_tag="PLG49_08625"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOQ17421.1"
                     /translation="MPKKNQVIDVTVEDLIFPNKGIAYIDGKKVIIKNAIKGQKVRAR
                     VTKKRKDKIEASIIEVLEKSPIEKEALCPHFGICGGCSYQTLPYEEQLKLKESQVKRL
                     LDNAGLEGYEFLDILPSPSEYNYRNKMEFTFGDVEKGGELALGMHKTGSFYEMVTVEG
                     CQIVHADFIKIMMAVLIYFRERKIPFYHTRKHEGYLRHLVIRRSAKLGEILVNLVTSS
                     QMNLDLSDLVEEVKNLKLEGELKGFLHTINDSLADVVQSDETRVLYGQDYFMEELLGL
                     KFKISAFSFFQTNSLGAERLYSVVRDFAGSTKDKIIFDLYCGTGTIAQIMADVSKKVI
                     GIEIVEEAVQAAKENAKLNGLENCEFIAGDVLAKVDELKDKPDLIILDPPREGVHPKA
                     IDKIIKFGAKQIIYVSCKPTSLARDLKIFVEKGYKVEKVRCVDMFPHTPHVET"
     gene            complement(1578..1955)
                     /locus_tag="PLG49_08630"
     CDS             complement(1578..1955)
                     /locus_tag="PLG49_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOQ17422.1"
                     /translation="MIKKVVTDKAPAAIGPYSQAIIYGNFLFTSGQIPIDPVTGEVVG
                     DDIETQANQVMKNLAAILTEAGTSFDKVVKVTCFLKDMNDFAKFNEVYTRYFTSKPAR
                     SCVAVKSLPKDVLCEVELIAVID"
     gene            complement(2610..4088)
                     /locus_tag="PLG49_08635"
     CDS             complement(2610..4088)
                     /locus_tag="PLG49_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ17423.1"
                     /translation="MYQDNYNTKRVHKVNILITIILIFLICIPIISSKGITGAMSSIV
                     AGILLLGIDLIAYFLPVKDYVKGFFLSLLPVIIILSLFVLQGYALNKHYIILLSIAMV
                     TLYFKKELILILGLFIDAAYLFLFFTFPENLLGVNNDLKGFFTVFFIINAIIILLYLL
                     TKWGRQLIEEAYQKDLESQKLVQRITSTFNATEEVSSLLDEHITNFKNDINTIYHSSK
                     GIIEAVEQMAIGIQEEAESVNLINDTMNHSMSKMDGAMNISKAIVTESQSMNIKVQEG
                     WQKINQVTNYIDNVASAISTTTQTVSDLQFSLDRVNNLLNGIQTIADQTNLLALNAAI
                     ESARAGEHGKGFAVVGEEVRKLAEQSTRITLDIAEVTTELFNKSKEAQEKSVQGDISI
                     KEGKKLLEEISRYFEELKDTYNIINEGLSNGMEEIAQAVENFSMIQSQIENVSAISQE
                     NAASTEEIISTIENEHALISSINAAVTDIKELSQKLHNMTQN"
     gene            complement(4205..4550)
                     /locus_tag="PLG49_08640"
                     /pseudo
     CDS             complement(4205..4550)
                     /locus_tag="PLG49_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
     gene            complement(4660..6393)
                     /gene="ade"
                     /locus_tag="PLG49_08645"
     CDS             complement(4660..6393)
                     /gene="ade"
                     /locus_tag="PLG49_08645"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOQ17424.1"
                     /translation="MEKSLLKKRIDTAAGRVPADMVIKNCKIIDVYNASIIEGQSIAI
                     TDGYIVGIGDYQGKHIIDAQGRYAAPGFIDGHIHIESSYVTPEEIGRLLVPHGTTTII
                     ADPHEIVNVCGLSGLNYMLEAARGTKLDILYMLPSCVPATPFENAGAVMYAESMKEPI
                     KDKRILGLGEFMNYTGVVEAEDDVLDKLQLAINEGKIIDGHSPALAGNALNAYVACGI
                     RTDHECSTVEEMLDRLSRGMYILLREGSACHDLRTLLKAVTPANSRRCLLCSDDRQPE
                     TIFRLGHLDNHLRICVEEGLSPIIAVQMASLNAAECYGLLDRGAIAPGLRADIVLLDN
                     LYDFKVHQVFIKGEEVAREGKYLPKIQRHDVSAVQGRFNVKDFSIGRLRLKSHSKKVY
                     VIDLLPGGVVTAKGTAEINLNEKGEFIWRPEQDIAKIAVVERHHGTGNVGLALLRGYG
                     IKAGAVALSIAHDSHNIITVGTNDEDMLLAVEELIKQGGGIILVKDGLVINSMPMVVG
                     GIMSDQSGEWVCEKLTQIHKDAYEQLGISKAVEPVMTLCFMSLAVIPEIKLTDKGLFD
                     ITQHAFISIEA"
     gene            complement(6609..7655)
                     /locus_tag="PLG49_08650"
     CDS             complement(6609..7655)
                     /locus_tag="PLG49_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17425.1"
                     /translation="MNIRCLYENLTCIKEIKVIGKNVKLNDVICNVMGIVRRGTELRL
                     LILRYDESFQRKIEENESTEWFDNPVSPKTNRLLLRGDREIYENNPFHSVLKVFIGEN
                     EFEVDYLEQQRLNTQDWESLMIIIKFLNAGWQADEIDYQDINMLFLTSIKLNGDYNSI
                     PDFNQNSELHFVMGPQSVAHLVEKPITLPIGGIYPEKLTFQDAMTGEEHWVQINRVYL
                     ADMWEETDKIFSNPKFQEQMSSEELVQMRLDFENKLLEICPKGMYFPVIEYECEEDIS
                     LQFYSKAFLDAKPVQKSSSMGFIIRPDKSEGILGFRLKAAVIQEPVPVNTVAVEAELF
                     QYIHTTAGRDIVLK"
     gene            complement(7753..8055)
                     /locus_tag="PLG49_08655"
     CDS             complement(7753..8055)
                     /locus_tag="PLG49_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17426.1"
                     /translation="MILFYSVEATKSFSSAIEFSDNSYRFYPFEILFLIYVRNKQGLP
                     VLDKFDDLLMSNPEAKMIFKDPEPYPQWDPLLRMIDDFYRRNYSDYFPNKYGSLFQ"
     gene            complement(8071..8454)
                     /locus_tag="PLG49_08660"
     CDS             complement(8071..8454)
                     /locus_tag="PLG49_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17427.1"
                     /translation="MARELYKTRYPNMSTWVGYDIGELIIALIHFTMFGWEKEENALF
                     DFITEQLGENIMNVNECRMHIWFLFELYLQYRNKTLLGTNQNVHQAVKNYFKGAELKY
                     LLIPEDLGIYKEVLDKWTTGDLDEI"
     gene            complement(8667..8813)
                     /locus_tag="PLG49_08665"
     CDS             complement(8667..8813)
                     /locus_tag="PLG49_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17428.1"
                     /translation="MQLEDMNELINSIGQDGTDTELSEENRTDYMKRNINAHLKSILK
                     IVRK"
     gene            complement(8803..9036)
                     /locus_tag="PLG49_08670"
     CDS             complement(8803..9036)
                     /locus_tag="PLG49_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17429.1"
                     /translation="MICEDKTFIDVFDWVPDADYDSRIGEWYVKVKENSGQIYVCAPY
                     VDTMTKQLVLTLSKEITLKERWKKSRFRSRHAT"
     gene            complement(9185..>9947)
                     /locus_tag="PLG49_08675"
     CDS             complement(9185..>9947)
                     /locus_tag="PLG49_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17430.1"
                     /translation="YLSDSVMRTDTGMDIYKFIYIIDGLVWLASCNVRGGEIVDLNVC
                     KKEHFNIVRQAIEFYRHDYSSSVLKEKFKKEHSGTSIYRYNDKLKKELLTLFKKELLA
                     YYGEPERALQNKLCEFCEILNQNVISAKYTVESFRVQDDDENDILPSRLINRDGDIWL
                     YFVGPADDTHLLSDNLETERLLFYYKGFVTCLFFSKNKTDKGFFKLNAFFVNKQEIDK
                     DELIEKLKENGIDSNEYHEILQIVLNNLHFNRYLV"
BASE COUNT         3047 a         2033 c         1372 g         3495 t
ORIGIN      
        1 cgtctccacg tgaggcgtgt gagggaacat gtctacacat ctcacttttt ctactttata
       61 tcccttctcc acaaaaattt ttaaatctct agccaaagaa gtcggcttgc atgaaacgta
      121 tatgatctgc tttgcaccaa actttataat cttatcaata gcctttggat gaacgccttc
      181 acgaggcggg tctaaaataa ttaaatcagg cttatctttt aattcatcta cttttgctaa
      241 tacatcacca gcgataaatt cgcagttttc cagtccgttt aattttgcat tttcttttgc
      301 tgcctgaaca gcctcttcta caatttcgat tccaatcact ttttttgata catcggccat
      361 aatttgagca atggttccag tcccacagta taaatcaaaa atgatcttat ctttagttga
      421 tcctgcaaaa tctcttacaa cagaatagag tctttctgct cctagagaat tggtttgaaa
      481 gaaagaaaaa gcagatattt taaactttaa accaaggagt tcttccataa aataatcttg
      541 tccataaagg accctggttt catcactttg aaccacatct gcaagactgt cattaatcgt
      601 atggagaaag ccttttaact ctccttcaag cttcaagttt ttaacttctt ctactaagtc
      661 cgataaatct aaattcattt gagaactggt cactaagtta actaaaattt cgcctaattt
      721 tgcacttctt cgtatgacaa gatgacgaag gtagccctca tgttttcgtg tatgataaaa
      781 aggtattttt ctttctctga aataaattaa cacagccatc ataattttaa taaaatctgc
      841 atggacaatc tgacagcctt ccaccgtaac catttcataa aaagagcctg ttttatgcat
      901 tcctaaagcc agttccccac ctttttctac atctccaaaa gtaaattcca ttttgttacg
      961 gtagttatat tctgaagggc tcggaaggat atccaaaaac tcatatcctt ccagtcctgc
     1021 attatctaaa agtcttttga cctgactttc tttaagtttt aactgttcct cgtaaggtaa
     1081 agtttgatag ctgcagccac cacatatacc aaaatgagga cataaagctt ctttttcaat
     1141 tggggatttt tcaagaacct ctataattga agcttcgatt ttatcttttc tcttttttgt
     1201 aacccgggct cttacttttt gtcccttgat tgcatttttt ataatgactt ttttaccatc
     1261 tatataagct atccctttat tcgggaaaat aagatcttca acagttacat caatcacttg
     1321 attctttttt ggcattttac tcctccgtac atttaatttt tgcaaaaaca cagggacggt
     1381 tccctgtttc attttgtttc attttgtttc attttgtttc attttctatg ctttcttatt
     1441 taattatgat aatctatcac cttttttaaa acaagaatta taaaagacag ttgccattct
     1501 ttttgtttaa ttgattactt gaattaagtt aaaaaaacga aagaactcct gcttcctgtc
     1561 attattaaag attataatta gtcaatgact gctattagtt caacttcaca aagaacatct
     1621 ttaggtaaac tttttacagc cacacaggac cgtgcgggtt tactcgtaaa atacctggta
     1681 taaacttcat tgaacttagc aaagtcgttc atatctttta agaaacaagt aaccttaacg
     1741 actttatcaa aagacgttcc tgcttccgtt aaaatagccg ctaaattctt catgacttga
     1801 ttggcctggg tttcaatgtc atctccgacc acttcacctg taacaggatc tataggaatt
     1861 tgaccagatg taaataagaa gtttccatat ataatggcct gagaatacgg accaattgct
     1921 gctggtgcct tgtctgtaac aactttcttt atcatttttt caacctccat tattttatat
     1981 tttacaaaga agcaaaggaa cggttctttt gcttcttgaa tgtttcttat gctgattcct
     2041 ttttttatca taatctatct atttcttgga aacaagaact attcaaaata atttcttctg
     2101 ctctgatctc atttaagttt tttatatatt tttaagaaac aaacttgttt taagcctgcc
     2161 aaatatatac ccatattcac aaaacagttc atacctttct ttatgaactg gtctcatatc
     2221 ctaactcctt ttaagttcaa attgtattat agaattaaaa acaaaaatac cgcaaaacag
     2281 tctaaaaatt actgcaaact ggtgcctgaa aataaacata caaaaagtac acctcttcga
     2341 tgtacttaaa attttcaata tatatgagag gctgcttagc ttaaaaataa agcgatccat
     2401 aagactataa ataaaatacg tgatttttac catgccaata tttagtgttt tgacattatc
     2461 acctcatgca aaaatgtcat aaacatcgtc tcctactctt ccacgcttac tattcttgtt
     2521 aaatatatta acgtccaaat tgtctatctt tcaatattat tgtaaaaaat gtgtaacaaa
     2581 agaaccgtcc ccttacttca cccttgttct taattttgtg tcatgttatg aagcttctga
     2641 cttaactctt ttatatctgt aactgctgcg ttaattgaac taattaatgc atgttcgttt
     2701 tctatcgttg atataatttc ttcagttgat gctgcatttt cctgtgagat tgcagataca
     2761 ttttctatct gactttgaat cattgaaaag ttttccacgg cttgtgctat ttcttccata
     2821 ccatttgata atccttcgtt aataatatta taagtatctt taagctcttc aaaatatctg
     2881 gaaatttcct caagtaattt tttcccttct ttgatcgata tatctccctg gactgatttt
     2941 tcctgggctt ctttcgactt attgaatagt tctgttgtta cctcagcaat atccagagta
     3001 atacgcgtgc tttgttctgc cagttttctt acttcttcgc caacaaccgc aaagcctttc
     3061 ccatgttccc ctgcccgtgc agattcaatg gcagcattaa gggccagtaa attagtctga
     3121 tcagcaattg tttgaatgcc atttaataag ttattcactc tatccagact aaactgtaaa
     3181 tcagaaaccg tctgtgtagt tgtactaatt gcggaagcaa cattatcgat ataatttgtc
     3241 acttgattaa ttttctgcca gccttcctga acctttatat tcatgctttg agattctgtt
     3301 actatagcct tactaatatt cattgcacca tccattttag acatagaatg attcatagta
     3361 tcattgataa gattaacgct ttctgcttct tcctgtatac caatggccat ttgctcaacg
     3421 gcttctataa tgcctttgct ggaatggtaa atggtgttaa tatcgttctt aaaatttgta
     3481 atatgttcat caagtaaact ggaaacttct tcagttgcat taaaagttga tgttattcgt
     3541 tgtacgagct tttgagactc aagatctttt tgataagctt cttctatgag ctgccgcccc
     3601 catttggtta atagatacaa caggataatt attgcattta ttatgaaaaa gactgtaaaa
     3661 aatcctttta aatcattgtt gacacctaat aagttttcag gaaaagtgaa aaacaaaaat
     3721 aaataagccg catcaataaa taatcctaaa attagaatga gctctttctt aaaatacaat
     3781 gtaaccatgg caatagataa aaggataata taatgcttat ttaaagcata accctgtagc
     3841 acaaaaagtg aaagaataat tatgacaggc aacaatgaca ggaaaaaccc ttttacataa
     3901 tctttgacag gcaaaaaata agcaatcaga tctatcccta aaagcaagat tccagctaca
     3961 atactcgaca tggctcctgt aatacccttt gaggatatta ttggaataca aattaaaaaa
     4021 attaaaatga ttgttattaa aatattgact ttgtgaaccc ttttagtatt gtaattgtct
     4081 tggtacatca aattccagcc tccatcctca ggtctaaaat aaaaatctcc taagcacatt
     4141 ctatcatctt aggagaaaaa ctgtaaatat ttaatttgag gaagtcagct ttactttttt
     4201 tctactattt tcttaaaatt ttttccatat cttttccctt tgccaactca tctattaatt
     4261 tgtctaaata acgaatgttc tgcatcattg gttcttcaat atcttctacc cggacaccac
     4321 aaacgactcc tttaattaaa ttccgtgcag gatttaattg gggtgcttca ttaaagaaag
     4381 tctcaacatc tacctgcctt tctaactgag cttctaactc cttctgactg taacctgtaa
     4441 gccagcagat aatctcatcg acttctgttt tcgtgcgccc tttttttttc tgcctttgag
     4501 acataaaggg gataaacttt tgaaaaactc attttataaa tcttctgcat gatataacac
     4561 ccctttcttg attatttttg ctccttactc catctccgcc tacttacaaa aaactaatac
     4621 gagttatgtc aatattacct ttttcctttt cagcttttct tatgcctcta tagaaataaa
     4681 agcatgctgt gtaatatcaa aaaggccctt atccgttaat ttaatttcag gtattactgc
     4741 cagtgacata aagcacaagg tcatcactgg ctctactgct ttactgatac caagttgttc
     4801 ataggcatct ttgtgaattt gtgtcagctt ttcacagacc cattcacccg actggtcact
     4861 cataattcca cccacaacca ttggcataga attgataacc agaccatctt tcacaaggat
     4921 aataccacca ccttgtttta taagttcctc tactgccagt aacatgtctt cgtcattggt
     4981 tcccaccgtt ataatattat gagagtcatg agcaatggac aaggccacag cacctgcttt
     5041 aattccatat ccacgaagca aagctaaacc tacatttccc gttccatggt gccgttctac
     5101 aactgctatt tttgctatat cctgttctgg gcgccaaata aactctcctt tttcattgag
     5161 attaatttct gctgtacctt ttgccgttac aacaccgcca ggcagcagat ctattacata
     5221 gacttttttt gaatggcttt ttaatcttaa tcttccaata gaaaagtctt ttacattaaa
     5281 gcgaccttgt actgctgaaa catcatgcct ttgtattttt ggtaaatatt tgccttccct
     5341 ggcaacttcc tcacctttta taaagacctg atgtacttta aaatcatata aattgtctaa
     5401 caatacaata tctgcgcgaa gccctggggc tatagcacct ctgtccagta agccatagca
     5461 ttccgctgca tttaagctgg ccatttgaac agcaataata ggagaaagtc cttcttctac
     5521 gcaaatacga aggtgattgt ccaggtgtcc caatctgaaa atggtttcag gctgccggtc
     5581 atcagaacat agcaggcaac ggcggctatt agctggtgta acagctttca gcaaggtacg
     5641 aagatcatga caagcagaac cttctcttaa aaggatatac attcccctgg aaagtcggtc
     5701 gagcatttct tctacagtag aacattcatg atcggtacga attccacaag ccacataagc
     5761 atttaaagca ttccctgcta aagctggact atgtccatca atgatttttc cttcattgat
     5821 ggcaagctgc aatttatcta aaacatcatc ctctgcctcc acaactcctg tatagttcat
     5881 aaattcgcct agtccaagaa ttcgcttatc cttaatcggc tctttcatac tttctgcata
     5941 cattaccgcc ccggcattct caaaaggcgt tgccggcaca caagatggaa gcatataaag
     6001 aatgtcaagt tttgttcccc tggctgcttc gagcatataa ttcagaccgc tcagaccgca
     6061 cacattcaca atttcatgag gatcagcaat aatggtggtc gttccatgag gaactaaaag
     6121 ccttccaatt tcttctggtg taacatagga ggattcaata tggatatgcc cgtcaataaa
     6181 acctggtgca gcatagcggc cctgtgcatc aattatatgc ttgccctgat aatcacctat
     6241 acccacaata tagccatctg taatggcaat actttggcct tcaataatag aggcattata
     6301 gacatcaata attttacaat ttttgattac catgtcggca gggactcttc ctgctgctgt
     6361 atcaattctt tttttcaata agcttttttc cattataata tctcctttat ttcaactttt
     6421 ttatatcaag ctgaccgacc aatagcaaca aggggacgat tccttgtttc tttttacact
     6481 atttataaat attcttttta atcataataa gctatctatt tttctaaaac aagaattatt
     6541 aaaaaaatac cttagattct tttgcatcta aggtatttta gcatttctaa ttatttatta
     6601 ttgtttctct acttcaatac aatatccctg ccagctgtag tatgaatata ttgaaataat
     6661 tctgcttcga ctgcaacagt attgactgga acaggttcct gtataacagc agccttaagc
     6721 ctaaatccca agataccttc tgacttatct ggtctaatta taaatcccat gctgctgctt
     6781 ttttgcacag gttttgcatc caaaaaagct ttagaataaa actgcaaaga aatatcttcc
     6841 tcacattcat actcgataac aggaaagtac atacccttag gacagatttc taaaagttta
     6901 ttctcaaaat ctaacctcat ttgcacaagt tcttcagagg acatttgctc ttgaaatttg
     6961 ggattcgaaa aaatcttatc tgtctcttcc cacatatctg ccaaatatac tctattgatt
     7021 tgtacccagt gttcctcacc agtcattgca tcctgaaaag ttaatttctc gggatagata
     7081 ccaccaatag gcagggtaat tggtttttct acaagatgag ccacactttg aggtcccatt
     7141 acaaaatgca gttctgaatt ctgattaaag tctggaatgg aattgtaatc accatttaat
     7201 tttatactgg ttaaaaatag catatttatg tcctgataat cgatttcatc agcttgccat
     7261 ccagcattca ggaatttaat aattatcatt aaagattccc agtcctgtgt attgagtcgc
     7321 tgctgttcca aataatctac ttcaaattca ttttcaccaa tgaatacttt taatactgaa
     7381 tgaaaaggat tattttcata gatttctcta tccccacgta atagcagcct gtttgttttt
     7441 ggacttactg ggttatcaaa ccattcggtt gattcatttt cctcaatctt tctttgaaaa
     7501 ctctcatcat accgtaaaat caaaagacgg agctccgtgc cacggcgtac tattcccata
     7561 acattacaaa ttacatcatt caatttaaca ttttttccta ttacctttat ctccttaata
     7621 caagttaaat tttcgtacaa acacctgata ttcatatact catagctccc tattcatgat
     7681 tacttaactt tttcccttat cctatcatcc agttccactc atttatcatt tcattgaatt
     7741 ttttacttat tatcattgaa acaatgaacc atatttattt ggaaaataat cagaataatt
     7801 tctgcgataa aaatcatcaa tcatacgcaa caaaggatcc cattggggat agggctcagg
     7861 atctttaaat atcatctttg cttccggatt actcatcaat aaatcatcga atttgtcaag
     7921 aacaggcaaa ccttgcttat ttcttacata aataaggaat aatatttcaa atggatagaa
     7981 tcgataactg ttatcactaa attctattgc tgacgaaaaa ctctttgtgg cttctacaga
     8041 gtagaataat atcatttgct ggatcaaatt ttaaatttca tctaagtcac cagttgtcca
     8101 cttgtccagt acttctttat aaatacccag atcctcaggg attaataggt acttaagttc
     8161 tgcaccttta aaataatttt tcactgcttg atgaacattt tgattggttc ctaaaagggt
     8221 tttatttcta tattgcaaat ataattcaaa taaaaaccaa atgtgcatac gacattcgtt
     8281 cacattcatt atattttcgc ccaattgctc cgtaataaaa tcaaataatg cattttcttc
     8341 tttctcccag ccaaacatgg taaaatggat tagcgctata ataagctccc cgatatcata
     8401 tcctacccag gtactcatgt tagggtaacg tgttttgtaa agttcccgag ccatatatta
     8461 gtaaaagatc gccttttggg cttgttttaa gttttctgtt tgacttctgc cattaataag
     8521 aacatcttct ttccttttag atatgtaata tgaaataata tcatctatat atataaacaa
     8581 aacatgctcc ctgtctaggt tgtttgactg aatgatatca gctatttcac tttcatcttc
     8641 atgataagat tcttcatcca gctctattac ttcctgacaa tttttaatat actttttaaa
     8701 tgtgcgttga tatttctttt catataatcc gtccgattct cctctgaaag ttctgtatcg
     8761 gttccatctt gtccaataga gtttattaac tcattcatat cttcaagttg catgtctgct
     8821 cctaaaacgg cttttcttcc atctttcttt taatgtaatt tcttttgata aggttaaaac
     8881 caactgcttt gtcatggtat cgacataggg ggcacacaca taaatttgtc cactgttttc
     8941 ttttacttta acataccatt ctccgattct ggaatcataa tctgcatctg gtacccagtc
     9001 aaaaacatca ataaaagttt tatcttcaca tatcaacgtt tattagaatg aattttaatt
     9061 ttttactgca caagaattaa aaaaaagcat aaaatcattt cacaatttgc attatactaa
     9121 tttgaataaa atcttctatt cttttcaaga gaataaaacg cctttttcaa ctactgagca
     9181 acattcatac aagataacga ttgaagtgga gattgttaag gactatttgt aaaatctcat
     9241 gatattcatt tgaatctatt ccgttttctt ttaatttttc aatcaactca tccttatcaa
     9301 tttcttgctt attgacaaaa aaagcgttta atttaaagaa gcctttatct gttttatttt
     9361 tagaaaaaaa caaacaagta acaaatcctt tatagtaaaa taataaacgc tctgtttcta
     9421 aattatctga taatagatgc gtatcatctg caggtccgac aaagtaaagc cagatgtcac
     9481 catctcgatt tatcaatcta ctgggtaaaa tatcattttc gtcatcatct tgtactctaa
     9541 aactttcaac cgtgtattta gccgatataa cattttggtt caaaatctca cagaattcac
     9601 agagtttgtt ctgcaatgcg cgttcaggtt caccataata agcaagaagt tcttttttga
     9661 aaagcgttaa aagctctttt ttaagtttat cattatacct atatatagaa gttcctgaat
     9721 gctctttttt aaatttttct ttaaggacac tagatgaata atcatgcctg taaaattcga
     9781 tggcctgacg cactatatta aagtgttctt ttttacacac attaagatca acaatctcac
     9841 caccccgcac attacatgaa gccagccaaa ccaggccatc aataatatat ataaatttat
     9901 aaatatccat gcctgtgtcc gtacgcatca cagaatctga aagataa
//
LOCUS       DAOSUD010000072         9886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3753_length_9886_cov_23.977215, whole genome shotgun sequence.
ACCESSION   DAOSUD010000072 DAOSUD010000000
VERSION     DAOSUD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3753_length_9886_cov_23.977215"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..527)
                     /gene="flgN"
                     /locus_tag="PLG49_08680"
     CDS             complement(69..527)
                     /gene="flgN"
                     /locus_tag="PLG49_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOQ17431.1"
                     /translation="MDLDETRVQLKILLDLLYKKKSILEQILTITENQFLIIHSDEKN
                     MDFLDEMVKEKSHLIDKVNEIDEHFLNLYNKISFQIKKNADLYKDIIKKLQERIKDIS
                     DTDAKIKIQEEKNRQELLININSKNRKRGLFKPKKEIIDAYKKYGLKKDH"
     gene            complement(548..928)
                     /gene="fliS"
                     /locus_tag="PLG49_08685"
     CDS             complement(548..928)
                     /gene="fliS"
                     /locus_tag="PLG49_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOQ17432.1"
                     /translation="MTQQNAYNNYRNNVIMTASPQELTLMLYDGALKFCRQAMLAIDE
                     GKIKEAHEYIVRVEDIIEEFQATLDKKYEISSSLDLLYDYIYRRLVEANVQKDKAILE
                     EVYGLIKELRDTWKEAMKLSKVKK"
     gene            complement(1040..1519)
                     /locus_tag="PLG49_08690"
     CDS             complement(1040..1519)
                     /locus_tag="PLG49_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17433.1"
                     /translation="MENKNLIEKMTLLAKEKQKELKAIWDITEEQAKNCTEDQLAQFM
                     ILLEKRQEHIDRISVIDKAFNESSNILKKNLQIQYLNQINQETYPLAGNLLRMIEEID
                     NMASRIKSLDDENKKRMDILLEDIKGKLKKIGEGKAANKLYQNGYSGSGGTFFDTKR"
     gene            complement(1521..3989)
                     /gene="fliD"
                     /locus_tag="PLG49_08695"
     CDS             complement(1521..3989)
                     /gene="fliD"
                     /locus_tag="PLG49_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOQ17434.1"
                     /translation="MLITSNKLRLTGLSGLDTEAMVESLMKAEFAKVDKMKQQLQKLK
                     WQQEGYREMINVLRGFKDTYLDVLSSKNIITTSNFRVYGVTAKINGSDTDAVKVEATS
                     SAGSASHSIVVKKLAQKEIWESISSVSGEMLGNDIDWDALKRGKTIDVTLDGTKKTIK
                     LSQDHSVLKDVDALVADLQSQFDKAFGSGNISVANVGGKISFVAEGHTFKLSNSINTY
                     VSSLGFNNNQSNAITGTKVTFPLDLSNGSFKVKVNGEERTITLSESAVYDESSLDVLA
                     ADLKAAINAAFDTDVVDVRIEENKLKIVSYNTSDEIVFTNGTENNILNKLGFSNNAKI
                     AKLEGAADISISEIGKEFDIYIDGVKNHIEITEEINSVADLANVINAQISGITVEADG
                     DKLVFKDTNGKEVRVSNSQLQSLKGMGFTDGDTNILNLDSSLSKAFGITEDTTVEING
                     VSLTFKATDTVRSMMNKINSSSAGVTLSYSSVKDKFILEAKQEGAANKIQVSDNVLFN
                     KLFNPTNDSNASYVIQEAADAEIILDGVETRRSSNTFEVDGLKYTLNRETGNEKIEIK
                     VSPEPEKLVEKIKDFVNAYNEMISKIAEKVNEKRVKSGSYEYYEPLTDEQKKEMSEKD
                     IELWEEAAKSGLLRNDATLQNILYSMRRALYDAVEGVGIRIFDIGITTSKEYTDNGKL
                     VIDEEKLRKAIEERPDEIAELFTKKSAISYDGGNRNTRYAENGLASRLLDIINDNIRT
                     TVNSKGYRGTLIEKAGIENTVSATNNILSKQIEKQSQEIEKMLDRLTEKENNYFLMFS
                     RLESMMQQLNTQSTFLASIIGGWQ"
     gene            complement(4001..4375)
                     /locus_tag="PLG49_08700"
     CDS             complement(4001..4375)
                     /locus_tag="PLG49_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOQ17435.1"
                     /translation="MKVENSVQTAQMTMQNIQNQKYGNLAQEQKLDNNQVVDQKQKST
                     DSNLTIAEKTVIEAIERANKKLSGVFAEFEFSIHEKTKQISIKVINKETKEIIREIPP
                     EQVLDMVAHLWEMAGIILDEKR"
     gene            complement(4496..5443)
                     /locus_tag="PLG49_08705"
     CDS             complement(4496..5443)
                     /locus_tag="PLG49_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOQ17436.1"
                     /translation="MNILVTGGAGFIGRWVVKRLLEDKHYVWVLDDLSNGREVNIEAF
                     YGHPCFKEFIKGDIKDKALLEKIFQNNFDICYHLGASINVQDSIDDPRTTFENDTVGT
                     FNVLEECKKYNTKMVFMSTCMVYDRAKDEKGITEFHPTKPASPYAGSKIAGENMVLSY
                     WYAYGLPVVVVRPFNTYGPFQKTGGEGGVIAIFIKRALEGEDLLIYGSGEQTRDFLYV
                     EDCADFVVEAGYNSKVNGEIINAGLGRDISINDLALLIAKDQSKIKHVPHIHPQSEIM
                     KLLCNYNKAQELLGWSPKYSLEEGIEKTKEWIASNSHLL"
     gene            complement(5486..6646)
                     /gene="pseC"
                     /locus_tag="PLG49_08710"
     CDS             complement(5486..6646)
                     /gene="pseC"
                     /locus_tag="PLG49_08710"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOQ17437.1"
                     /translation="MRDDFLPYGRQWIDEDDIQAVVNVLKSDYLTTGPAIKEFEDQLK
                     SVTGAEYVVAISNGTAALHAACFAAGIGEGDEVITTPMTFAATANAVLYCGGTPVFAD
                     IDPETYNIDPEDIRRKITDKTKAIIPVHYTGQPCDMDEIMKIAKEHNLIVIEDAAHAL
                     GAEYKGRKIGSIGDMTTFSFHPVKPITTGEGGAITTNNKEFYDKLMLFRSHGITRDQK
                     MLLNDNAAWYYEQHFLGYNYRMTDIQAALGCSQLNKLDYFLEKRREYAKLYNDAFKDC
                     EGVIRPRQLPDTLSGWHLYVLQLDLDKIRPDRRVIFDELRIHNIGVNVHYIPVYYHPY
                     YQKLGYQRGLCPEAEKLYERIISLPLFPKMTKEDIMYVIKCVKEIIGVYIKR"
     gene            complement(6651..7634)
                     /gene="pseG"
                     /locus_tag="PLG49_08715"
     CDS             complement(6651..7634)
                     /gene="pseG"
                     /locus_tag="PLG49_08715"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HOQ17438.1"
                     /translation="MMKILIRADGGKTIGMGHIMRTSVLAEELRRCAEVSYVCSEEEE
                     FDFGVNFLRQNGYFVYRFSEENLLSKINDIAYDCMIVDSYNVDANFFKSVKRAGSFLG
                     YIDDLKKEEYPVDFIVNQNPYALDLNYTEENYIETFLGITYVLLRKEFQNPMKRYISP
                     VCKDVLITVGGSDINRITEKIIQNILKEELDMTLHVVIGPAFPKGYLDTFSHPKVKLY
                     YNPIMSDLMKKCDLAVSACGSTVYELAACGTPTIGIVVADNQMMAYEKLKQLGIIKGS
                     NVENILDSIKEMTYEKRLEMSRKGQELVDGLGAKRLASEIFNLINERITGV"
     gene            complement(7646..8824)
                     /locus_tag="PLG49_08720"
     CDS             complement(7646..8824)
                     /locus_tag="PLG49_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17439.1"
                     /translation="MARIVLLKGRSQYDVLRIFVDYLGEAFNQLGDEIFIVDLTNPNR
                     MKALQDAFSFPCDFVFAFNCIGIDLKLDDEYLFNLADTTYIGCLVDHPIYHLQRINTN
                     LNNLILTCVDYSHIEFLCTYFPLKRSAFLPHGGSVKGEDIKEQRIRDIDVLFGGSFKD
                     PDLIRKEWLILPKTMISIMDEAAEYLLANPDKTLEGGLQFVLQSRNFYGEYGILMNNP
                     AFLQLVDSYVRSYRRYKCVEELIKSGIPIDFYGVLGWDRNPFEMYKNFRVHNSVDFNE
                     MLSLMGNSKFVLNVLPNFKNGSHERIFTSMLQGAISVTDKNQYLENQFNDEEDIIFYT
                     WDKIELLPEKIKDILEDEDNRNYIALNGMKKANEKHTWLQRAKEIKELVEINSVFRTP
                     "
     gene            complement(8856..9593)
                     /locus_tag="PLG49_08725"
     CDS             complement(8856..9593)
                     /locus_tag="PLG49_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOQ17440.1"
                     /translation="MDNGIICIIQARMGSERLPGKVIKEIKGTPMILHILNRLNRANK
                     IEKTIVATSTNSENDRLEDVVKAAGGNIFRGSEDDVLKRYVDAAEQFRGKYIIRVTGD
                     CPLISAEVIDELIDKFLEAKVDYMRVDVPNTFSRGFDAEIFTREALMKAHYKATEARY
                     REHVTLYMYEHPEEFSVERMEAKESWNRPNYRLCVDTKEDFDLVEKVYEALYDQNKGF
                     EIDEIISYLDNHPEVAGINQFIIQKNV"
     gene            complement(9610..>9886)
                     /locus_tag="PLG49_08730"
     CDS             complement(9610..>9886)
                     /locus_tag="PLG49_08730"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOQ17441.1"
                     /translation="LKAMVKGIRDAEKAIGSSVKGLADTERLHHQRGRRSIFAKTDIP
                     KGTIITKDMLAVLRPGVGLMPKYFDIVIGREAKRDIKKNEPITWEVI"
BASE COUNT         2833 a         2056 c         1314 g         3683 t
ORIGIN      
        1 taatataaca tggagtggct aattagtcaa tatataaata aaaaatttta ttgattatct
       61 cataaggttt agtgatcttt tttaaggcca tattttttat aagcgtctat gatttctttt
      121 tttggtttga ataagccccg ttttctattt ttagaattta tattgatcaa caattcctgc
      181 ctgttttttt cttcctgaat tttaatttta gcatctgtgt ccgaaatatc ttttatgcgc
      241 tcctgcaatt ttttaataat atctttatat aaatctgcat tcttttttat ctgaaaagaa
      301 attttattat ataagtttaa aaaatgttca tcgatttcgt tgaccttatc aattaaatga
      361 cttttttctt taaccatttc gtccagaaaa tccatgtttt tttcatctga atgaatgatt
      421 aaaaattgat tttctgtgat ggtcagtatc tgttccagga tgcttttttt cttatataat
      481 aaatcaagaa gaattttcaa ttgaaccctc gtttcatcta agtccatact atcacctgta
      541 aaatcgacta cttcttaacc tttgacaact tcatggcttc tttccaggta tctcttaatt
      601 ccttgattaa tccatatact tcttctaaaa ttgccttatc tttttggaca tttgcttcaa
      661 ctaaacgtct gtagatatag tcatataata agtctaaact gctgctgatt tcatatttct
      721 tatccagagt tgcctggaat tcttcaataa tatcttctac acggacaata tattcatgcg
      781 cttcttttat tttgccctca tctattgcta acatggcttg tctgcaaaat ttcagtgcac
      841 catcataaag cattaaagta agttcctgag gagaagcagt cataataaca ttatttctat
      901 agttgttata agcattttgc tgagtcatac ggattctcct ttctgtctga ccctgcatag
      961 agggtctttt taattttagc taaaaacttt ctcatcgtga taaagaaaaa ttgctcttgg
     1021 ccatcaaagt aagagcaatt tatctttttg tatcaaaaaa tgttcctcct gaacctgaat
     1081 agccattttg ataaagctta tttgctgcct tgccttctcc aattttcttt aattttccct
     1141 ttatatcttc aagcaagata tccattcttt ttttgttttc gtcatctaaa gactttatcc
     1201 ggctggccat attatcaatt tcttcaatca tccttaataa attaccagcc agcggataag
     1261 tctcctgatt aatttgattt aaatactgga tttgtaagtt tttctttaaa atattggagc
     1321 tttcattaaa agctttatca attacgctaa tcctatctat atgttcttga cgcttttcta
     1381 aaagaatcat aaactgtgcc agttggtctt ctgtacaatt tttggcttgc tcttcagtaa
     1441 tgtcccagat cgcttttaac tctttttgtt tttcttttgc taatagtgtc attttttcaa
     1501 ttaaattttt attttccata ctattgccaa ccgcctatta tacttgctaa aaatgttgat
     1561 tgagtattta attgctgcat catagactcc aatctggaaa acatcaggaa ataattgttt
     1621 tctttttctg ttaatctgtc tagcattttt tcaatttcct gactttgctt ttctatctgc
     1681 tttgataaga tattatttgt agcagaaacc gtattttcta ttcctgcttt ctctatcaaa
     1741 gtacctctgt aacctttgct attaacggtt gttctgatgt tatcattaat tatatctaac
     1801 aaccttgagg ccagaccgtt ttcagcatat ctggtatttc gattcccgcc atcataagaa
     1861 atcgctgatt tctttgtaaa taactctgca atttcatctg gtctttcttc tatggctttt
     1921 ctcagctttt cttcatctat aaccaattta ccattgtcag tatattcttt ggatgttgta
     1981 attccaatat caaaaattct tattcctaca ccctctacgg catcatacaa agctcttctc
     2041 attgaatata aaatattctg caaggtagcg tcatttcgaa gtaagcctga ttttgctgct
     2101 tcttcccata attcaatatc tttttcggac atttcctttt tctgctcgtc cgttaaaggc
     2161 tcataatatt cgtatgaacc ggattttacc ctcttttcat tgaccttttc agcaattttg
     2221 ctaatcattt cattgtaagc attaacaaaa tctttaatct tttccactaa tttttcaggt
     2281 tccgggctaa ctttgatttc tatcttttca ttacctgttt ccctattaag agtatatttc
     2341 aacccatcca cttcaaaagt attggaactt ctcctggttt caacaccatc taaaataatt
     2401 tcagcatccg ctgcttcctg aataacataa gaagcattgg aatcattcgt aggattaaat
     2461 aatttgttaa aaagaacatt atcacttacc tggattttat tagctgcgcc ctcctgctta
     2521 gcttccagaa taaatttatc ttttacactg ctataagaaa gggttacacc cgcactactg
     2581 ctgttaatct tattcatcat gcttcttact gtatctgttg ccttaaaggt cagggaaacg
     2641 ccattgattt ctactgtagt gtcttctgta atgccaaaag cttttgacaa actgctgtct
     2701 aaatttaaga tatttgtatc tccatcggta aaacccatcc cttttaaact ttgcagttga
     2761 ctgttgctaa ctcttacttc ttttccattg gtatccttaa atactaattt gtcgccatct
     2821 gcttcaacag taattcctga aatttgtgca tttattacat tcgctaaatc agccacactg
     2881 ttaatctctt ctgtaatttc aatgtgattt ttaacaccat caatgtaaat atcaaattct
     2941 ttacctattt cagatatgga aatatctgct gcgccctcta atttcgcaat tttagcatta
     3001 ttactaaaac ctaacttatt taagatgtta ttttctgtcc cattcgtgaa aacaatttca
     3061 tcggatgtat tgtatgatac gatttttaat ttattttctt ctattcttac atctaccaca
     3121 tctgtatcaa aagccgcatt aattgctgct tttaaatctg ctgccaatac atccagtgaa
     3181 gattcatcat atactgcaga ttcagataaa gtaatggttc tttcttcccc atttacttta
     3241 accttaaaac tgccattaga taaatctaag gggaaagtta ccttagtgcc cgtaattgca
     3301 ttgctttggt tattattaaa acctaaggaa ctaacatatg tattgattga attagataat
     3361 ttaaaggtat gtccttctgc cacaaagcta attttccctc caacattagc tacactaata
     3421 ttaccggaac caaaagcctt atcaaactga ctttgtaaat ccgctaccaa agcatcaaca
     3481 tcttttaaaa cagaatgatc ttgggaaagc ttaattgtct tttttgttcc atctaaagtc
     3541 acatcaatgg tcttgcctcg ttttaaagca tcccaatcaa tatcattgcc aagcatttct
     3601 cctgaaacac tgctgatact ttcccaaatt tccttttgag ccaatttctt aacaacaata
     3661 ctatgacttg ctgatcctgc cgaactggtt gcttcaacct taacagcatc tgtatcactg
     3721 ccattaattt ttgctgttac accataaacc cggaaattac ttgttgtaat gatgttttta
     3781 ctggacagta catctaaata agtgtcctta aaacccctaa ggacattgat catttcacgg
     3841 taaccttctt gttgccattt cagtttttgc agttgctgct tcatcttatc gactttagca
     3901 aattcagcct tcataaggct ttcaaccata gcttctgtat ccagccctga aagacccgta
     3961 agacgaagtt tattacttgt tatgagcatg ttctcactcc ttatctcttt tcatccaaaa
     4021 taatccccgc catctcccaa agatgcgcta ccatatctaa tacttgttcc ggaggtatct
     4081 ctcgaataat ttcttttgtt tctttattaa tgacttttat agagatttgc tttgtttttt
     4141 catgaataga aaattcaaat tctgcaaata caccactgag ttttttattt gccctttcaa
     4201 tagcttcaat cactgttttt tcagctatag tcagattact gtctgtactt ttttgctttt
     4261 gatccacaac ttggttatta tcaagttttt gttcttgagc aagatttccg tatttttgat
     4321 tttgtatatt ttgcatcgtc atttgcgcag tttgaactga attttcaact ttcatttgtt
     4381 acacctccgt atcctttatt attatacttt accggcatat tatctactat ttatatcggc
     4441 attcaaccaa acattcttaa tataattcac tatatttaat aaaatttatg tgaaactata
     4501 acagatggct gttgcttgct atccactctt ttgttttttc aataccttcc tccaaagaat
     4561 atttcggact ccagcctaaa agttcttggg ccttattata attgcacagc aatttcatta
     4621 tttcgctttg gggatgaata tgagggacat gctttatctt gctttgatcc tttgcaatga
     4681 gcaaagccag atcattaatg ctgatatctc tgcctaaacc agcattgata atctcaccat
     4741 ttactttact gttgtagcct gcttctacaa caaaatctgc acaatcttct acatataaaa
     4801 aatccctggt ctgttcgcca ctgccgtaaa taagcaagtc ttccccctct aaggctctct
     4861 taataaaaat agcaataaca ccgccttctc ctcctgtttt ttgaaaaggt ccataagtat
     4921 taaagggcct tacaactact acaggaagcc catatgcata ccaataggaa aggaccatgt
     4981 tttctccggc aattttactg cctgcataag gagaggctgg tttagtcgga tgaaactctg
     5041 ttatcccttt ttcatccttt gccctgtcat agaccatgca agtactcata aataccattt
     5101 ttgtattgta ctttttacat tcttccaata cattaaaagt tcctactgta tcattttcaa
     5161 aggttgtcct gggatcatcg atactgtcct gaacattgat actggccccc agatggtagc
     5221 aaatatcaaa gttattttga aagatttttt ccaacagggc tttatctttt atgtccccct
     5281 taatgaattc tttaaagcag ggatgtccat aaaacgcctc tatattgact tctctgccat
     5341 tggataagtc atctaaaacc catacataat gcttatcctc taataatctt tttacaaccc
     5401 agcggccaat aaagcctgct cctccggtta ctaagatatt caaaatatca ctcctcagtt
     5461 atgacttcca ataaaaagct cttgtttatc gttttatata aactccaata atttctttta
     5521 cgcactttat tacatacatt atatcttcct ttgtcatttt aggaaatagc ggcaagctga
     5581 taattctctc gtataatttt tctgcttcag ggcacaaacc tctttgatag cccaatttct
     5641 gataataagg atgatagtaa accgggatat aatgaacatt gaccccaata ttatgaattc
     5701 ttaattcatc aaatatcact cttctgtctg gtcttatttt atccaaatca agttgcaaaa
     5761 cataaagatg ccagcctgaa agggtatctg gcagttgtct gggtctaatg accccttcac
     5821 aatctttaaa agcatcatta tagagctttg catattctct tctcttttca agaaaataat
     5881 ccaatttatt aagctgactg cagccaaggg ccgcctgaat atccgtcatt ctgtaattgt
     5941 atcctaaaaa gtgctgttca taataccaag ctgcattatc atttaaaagc atcttctgat
     6001 ctctggtaat accgtgagag cgaaagagca ttaatttatc ataaaattct ttattgttgg
     6061 tggttatggc tcctccttct cctgttgtaa tgggtttaac aggatgaaag ctaaaggttg
     6121 tcatatcccc aatacttcca atttttctgc ccttatattc agcacctaaa gcatgggctg
     6181 catcttctat gacaatcaaa ttgtgttctt ttgctatttt cataatctca tccatatcac
     6241 agggttgccc tgtataatga acgggtataa tcgcctttgt tttatctgtt atctttctcc
     6301 taatgtcctc cggatcaata ttataggttt caggatcaat atctgcaaat acgggtgttc
     6361 ctccgcagta aagtactgca ttggcagtcg ctgcaaaagt cattggcgtt gtgatgactt
     6421 catctccttc accaattcct gccgcaaagc aagcagcatg aagggctgct gttccattgg
     6481 atatggcaac cacatattct gctcctgtca ctgactttaa ttgatcttca aattctttta
     6541 tggccggacc tgtggttaaa tagtcgcttt ttaatacatt tacaacagcc tgtatatcat
     6601 cttcatcaat ccactgacgc ccatagggta aaaaatcatc tctcattttg tcacactcct
     6661 gttattcttt cgttgatcag attgaatatt tcggaagcta aacgcttagc tcccaacccg
     6721 tcaaccaatt cttgtccttt cctggacatc tcgagtcttt tttcataggt catttcttta
     6781 attgaatcca gtatattttc aacatttgat ccttttataa tacccaattg ttttaatttt
     6841 tcatatgcca tcatttgatt atctgctact acaattccta tagtaggcgt gccgcaggct
     6901 gccagttcat atactgtaga accacaggct gaaacagcta aatcacattt tttcattaaa
     6961 tctgacataa taggattata atataattta accttagggt gactgaaggt atctaaatac
     7021 cccttaggaa atgccggtcc aatgactaca tgaagggtca tatccaactc ttcttttagg
     7081 atattttgaa taattttttc tgtaattcta tttatatcag aaccccctac tgttatcaga
     7141 acatctttac atacagggct tatatatcgt ttcattggat tttggaactc ttttctaagc
     7201 aaaacatatg taatgcctaa aaatgtttct atataatttt cttctgtata gtttaaatct
     7261 aaggcatagg ggttttgatt gacaataaaa tcaacgggat attcttcttt ctttaaatca
     7321 tcgatatatc ccagaaaact tcctgccctt ttcacacttt taaaaaagtt tgcatctaca
     7381 ttataactgt ctacaatcat acaatcatag gctatatcat taatcttgct taataagttt
     7441 tcttccgaaa atcgataaac gaaatatcca ttttgtctta agaagttaac gccaaaatca
     7501 aattcttcct cttctgagca aacataggaa acctctgcac atcgtcttaa ttcctcagcc
     7561 aatacagaag ttctcataat atgccccata cctatggttt tgcctccatc tgcgcgaatc
     7621 agaattttca tcattaaacc taccttcagg gagttctaaa tacactgttt atttccacta
     7681 actctttaat ttcctttgct ctttgcaacc aggtatgctt ttcatttgct tttttcattc
     7741 cgttcaaagc aatataattc ctattgtcct catcttctaa tatatcttta attttttccg
     7801 gtaaaagctc tattttatcc caagtataaa aaataatatc ttcttcatca ttaaattgat
     7861 tttctaaata ttgattttta tctgttacag atattgctcc ttgaagcata gaagtaaaga
     7921 ttctttcatg agagccattt ttaaaattag ggagtacatt taagacaaat tttgaatttc
     7981 ccattagaga aagcatttca ttaaaatcca cagaattatg cactctaaag ttcttataca
     8041 tttcaaaagg atttctatcc catcctagta caccataaaa atctattggt attccagatt
     8101 ttatcaattc ttcaacacat ttatatctgc gataactcct tacgtagcta tccactaatt
     8161 ggagaaatgc aggattattc attaaaatac catattctcc ataaaaattt cttgattgga
     8221 gtacaaactg aagtcctccc tctaatgttt tatctggatt tgccaataaa tattctgctg
     8281 cttcgtccat tatagaaatc atggttttag gtaaaatgag ccattcttta cgtatcaaat
     8341 caggatcttt aaaacttcct ccaaataata catctatatc tcttattctt tgctctttaa
     8401 tatcctcccc tttaacagag cctccatgag gtaaaaatgc agatctcttg agtggaaaat
     8461 atgtacacag aaattcaata tgagaataat ccacgcatgt caaaataagg ttatttaaat
     8521 tggtattaat tctttggagg tgataaattg gatgatctac taagcagcca atataagttg
     8581 tatcagcaag attaaacaaa tactcatcat ctaattttaa atctattcct atgcaattaa
     8641 acgcaaatac gaaatcacac ggaaaagaaa aagcatcttg taacgctttc attctattgg
     8701 ggttcgttaa atctacaata aatatctcat cacctaattg attaaaggct tctcctaaat
     8761 aatcgacaaa tattctaagc acatcatatt gggatcgccc ttttaataat acaattctag
     8821 ccataaaacc ctcctattag atttcagcaa gattcctata catttttttg aataataaat
     8881 tggttgatcc ctgccacttc aggatggttg tctagatatg aaataatctc atctatctca
     8941 aaccctttat tctgatcata taaagcctca tatacttttt ctacaaggtc aaaatcttct
     9001 ttagtgtcaa cacataatct ataattcggt ctgttccagc tttcctttgc ttccattctt
     9061 tcaacagaaa attcctctgg atgctcatac atatataagg ttacgtgttc cctatatctt
     9121 gcttctgtgg ctttataatg ggctttcata agggcttctc ttgtaaatat ctccgcatca
     9181 aaaccccggc taaaggtatt tggaacatcg actctcatat agtctacctt tgcttctaaa
     9241 aacttatcaa ttagttcgtc aataacttct gcactaataa gaggacaatc cccggtaact
     9301 cttataatat atttccctct aaactgctcc gctgcatcta catatctttt cagcacatca
     9361 tcttccgagc ctctaaagat atttcctcct gcagccttta caacatcttc caatcggtca
     9421 ttttcagaat tggtcgaagt agcgacgata gttttttcaa ttttattagc tcgatttaat
     9481 cgatttaata tgtgaagaat cataggagtt cctttaatct cttttatcac tttccccggc
     9541 aatcgctctg accccattct ggcctgaata atacaaataa tgccattatc catagtaatc
     9601 cttctttcat taaataactt cccaagtaat gggttcattc tttttgatat ctctttttgc
     9661 ttctcttcct attacaatat caaaatactt tggcatgagc ccgacaccgg gtctgagcac
     9721 tgccagcatg tcttttgtta ttatagtccc tttgggaata tccgttttgg caaagatact
     9781 tcttcgccct ctttgatgat gcaatctttc tgtatcggcc aatcctttaa cagaagaacc
     9841 aatagctttt tccgcatctc taataccttt taccatagct tttaat
//
LOCUS       DAOSUD010000073         9801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3797_length_9801_cov_24.576442, whole genome shotgun sequence.
ACCESSION   DAOSUD010000073 DAOSUD010000000
VERSION     DAOSUD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_9801_cov_24.576442"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..175
                     /locus_tag="PLG49_08735"
     tRNA            101..175
                     /locus_tag="PLG49_08735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Pro,seq:tgg)
     gene            181..251
                     /locus_tag="PLG49_08740"
     tRNA            181..251
                     /locus_tag="PLG49_08740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:213..215,aa:Gly,seq:tcc)
     gene            257..330
                     /locus_tag="PLG49_08745"
     tRNA            257..330
                     /locus_tag="PLG49_08745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:291..293,aa:Arg,seq:tct)
     gene            350..423
                     /locus_tag="PLG49_08750"
     tRNA            350..423
                     /locus_tag="PLG49_08750"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:His,seq:gtg)
     gene            434..505
                     /locus_tag="PLG49_08755"
     tRNA            434..505
                     /locus_tag="PLG49_08755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Gln,seq:ttg)
     gene            514..586
                     /locus_tag="PLG49_08760"
     tRNA            514..586
                     /locus_tag="PLG49_08760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Lys,seq:ttt)
     gene            592..672
                     /locus_tag="PLG49_08765"
     tRNA            592..672
                     /locus_tag="PLG49_08765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:626..628,aa:Leu,seq:tag)
     gene            complement(710..1168)
                     /locus_tag="PLG49_08770"
     CDS             complement(710..1168)
                     /locus_tag="PLG49_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOQ17442.1"
                     /translation="MEIERKYLVKRLPENLHLYEKEEIIQGYISIDPIIRLRKSNDNY
                     CLTIKSKGHLKREEVEFPLTKEQFESLWQKVDSNTIYKSRYNIPLENNLVAELDVYHG
                     DLEDLLTVEVEFESEKASEKFTPPDWFGKDVTHDNRYKNSNLAVYGIPKD"
     gene            1643..3478
                     /locus_tag="PLG49_08775"
     CDS             1643..3478
                     /locus_tag="PLG49_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ17443.1"
                     /translation="MLKRFWAMSLVLVMVFAIALTACSKSDDNQTVGEAQQNTNNEDT
                     KEEYQGEKVLDYYLSSEPQTLDPQQMWGQPDIMVENMFMEGLMRFGKDDSSLEPGVAK
                     GYTYDEASNTYTFELREDAKWEDGTPITADDFFFAWRLAVDTAAPYSFLITDYIEGAA
                     DYAAYDKASFLAEKDAAFANLSEEEQAARIEAMADDELKQYNAKKDELWSQVKAVAEG
                     SNIKITLSIPAPYFPSLTAFAVYTPAREDFYKEKSAKNKYGIEAEGLLANGPWKVEEW
                     VHKDYFKLVKNENYWNKDNINIDVINLKIVNEVETRTNLLKTGALDGSAIQSKDVPDF
                     EDMATLEELNLQPMINRSDFSVFYIDFNHFDNPIIQNKNIRKALMYAMDRTSFVEKIN
                     IGDEPALAFVPKVFPGLEKTFREENGMELFKDNDKEKAKEYLEAGLKELGLTELPPLD
                     MLIGDSDIDQKVGEKFQADWNEIGITVNLVPLPWGERLTRLQKGDFAMSSSGWGPDYP
                     DAMTFLELFESVNPNNNGQYNNPEYDKLIKAAKNEKDAKKRIQYLYDAEKMLMEDAVI
                     VPQYFRNVHFTFKKYVTGVVIRGVGATTDFYWADIDMAAKAAEKK"
     gene            3617..4546
                     /locus_tag="PLG49_08780"
     CDS             3617..4546
                     /locus_tag="PLG49_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17444.1"
                     /translation="MLKYILKRLLMALATLWVISTLTFVLMHALPNDPFTDPKLKPEV
                     RQALRVKYGLDKPPSEQYVIYMKNLLKGDLGTSIKYPGRSVSDIIAESFPKSFALGWR
                     ALIIAVIFGMLLGIIAALNHQKTLDYLSIFIAIIGVSIPSIILGPILAYFFGVKLGWF
                     PVTVDKSEISLFLPSLTLAMSSLATISRLMRTTTLEVLGQDYISTAKAKGLSKTQIVL
                     KHVLRNAVMPIITILGPLFAALITGSIVVEKIFAVAGLGEYFVSTITEQDYPMIMGIT
                     IFYAALIILSILVVDIMYGFIDPRLRISGKGGE"
     gene            4547..5833
                     /locus_tag="PLG49_08785"
     CDS             4547..5833
                     /locus_tag="PLG49_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17445.1"
                     /translation="MMNTDMDRNEFKHVGKNLKASQEIKRPSLSYWKDAYRRLKNNKV
                     AMLSFWVLVILIIMSAIGPVITKNLYGHTYESQNLAEQNQTPIMTNARSIALLANDVF
                     EYDKYLSSYRKDKTTFKNVRLKSPGKIGFKIGNAADESWQGDKKEYQLMIEVSSSDTW
                     SSIIEKLNNEGQRLLQEDPDFRGIKFEKKGSNLVIETVGEKWFNKTHWFGTDEFGRDL
                     FTRLWEGGRISFFIAFISVFIASIFGIIYGGLSGYIGGKVDEILMRIVEILMTVPDML
                     YTILLLTVMDPGIKPISIVLIVTGWMGTARIVRGEVMRLKHSEYVVAAKTLGADTKKI
                     VFEHLIPNAMGPIIVNMTMMIPKMIFAEAFLSFIGLGVPVPYASWGSLANQGARIFRQ
                     FPHQLLIPAIAISLTMFAFNLLGDGLRDALDPRLRK"
     gene            5848..6897
                     /locus_tag="PLG49_08790"
     CDS             5848..6897
                     /locus_tag="PLG49_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17446.1"
                     /translation="MKKILEVKNLEVSFDTYAGEVQAVRDVSFDLYEGETLAIVGESG
                     SGKSVTAKSLIRLNPEPPARYKAGEILLHGENILDKKEKEMQKIRGSKIGMIFQDPMT
                     SLNPTMTVGKQIMEALMKHQKVAKSEARKVALEMLRMVQIPNPEKRFDEYPHQFSGGM
                     RQRVMIAIALVCNPQILIADEPTTALDVTIQAQILDLMKNLQEKIKTSIILITHDLGV
                     VAHSAQRIIVMYGGKIVESGTTDQIFYKSRHPYTWGLLNSVPRLDIHKEDGRLDSIEG
                     TPPDLFAPPQGCPFADRCEYAMEICRKKMPPQFETGENHHSACWLNHEFAPKVENPTQ
                     IAKKLQKEEVQMREQ"
     gene            6887..7870
                     /locus_tag="PLG49_08795"
     CDS             6887..7870
                     /locus_tag="PLG49_08795"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOQ17447.1"
                     /translation="MSNDREVLIKVRNLKKYFNIDKNIYLKAVDDVSFDIYKGETLGL
                     VGESGCGKSTIGRTILNIYKATSGTVEFKGKNIYELKGKEAKEFTRKAQMIFQDPYAS
                     LNPRMLVSNIIGEGIDIHKLLTGQARMNRIHELLELVGLNKEHANRFPHEFSGGQRQR
                     IGIARALAVNPEFIVCDEPISALDVSIQAQVVNLLKDLQEKMGLTYLFIAHDLSMVRY
                     ISDRVAVMYLGSMMELANSDELYENPLHPYTKALLSAIPIPDPKVQRKRERIVLQGDV
                     PSPINPPKGCKFADRCPMATEKCKNERPKLEEVKENHFVACHYWKEAEKRI"
     gene            complement(8066..8506)
                     /locus_tag="PLG49_08800"
     CDS             complement(8066..8506)
                     /locus_tag="PLG49_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17448.1"
                     /translation="MDSSYSYHFCKYSFLQVYQKNIEKKLQEIDLFLKITPEQLNIEN
                     TSALLDISVEEIKDLMSKYNIDSINPSSFFIIMVTGSSYICGLLRRQMQRGYKKIYTK
                     EDIAYIYQINPDKIEQALNQAQICEITGDNLKELFKYIPVCIAE"
     gene            8689..8916
                     /locus_tag="PLG49_08805"
     CDS             8689..8916
                     /locus_tag="PLG49_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOQ17449.1"
                     /translation="MANNNTNSRRNAVPQARAALDKFKFEVANEIGVPLKQGYNGDLT
                     SAQAGYVGGYMVKKMIERQEQQMAGNTNNLQ"
     gene            complement(9006..9644)
                     /locus_tag="PLG49_08810"
     CDS             complement(9006..9644)
                     /locus_tag="PLG49_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17450.1"
                     /translation="MNKVKNSLVFVLVLLVCLSTAACSKKELTLGEFQGNTYTNSYFG
                     ISMNIPEGWEIQNDETVNELMEAGKEAIAGDNAAKKAALDLAEQKLLTFFMICKYPIG
                     ELGKINPNVICQAEKVGSLVKNESEYLEAIRNQLESASQTPYNFKDIYTENLGGEDFY
                     VLESNIDFGSFVYTQKYYTKIMDKYAFNIVVGYTEEEGKEEISQMLESVIFE"
BASE COUNT         3309 a         1521 c         1844 g         3127 t
ORIGIN      
        1 acaaaaacgc ttccatcgac agattgagga gcttttgatt tttttaaaaa actattgacg
       61 aataaattgc cataagctat aataatcgtt gtcggtattt cggggtgtgg ctcagtttgg
      121 ctagagcact tgatttggga tcaaggggtc gcaggttcga atcctgtcac cccgatattt
      181 gcgggtgtag ttcaatggta gaacaccagc cttccaagct ggatacgtgg gttcgattcc
      241 catcacccgc ttttctgtgc ctgtagctca gctggataga gcaatggcct tctaagccat
      301 gtgtccgggg ttcgaatccc tgcaggcacg tcattttatt gccagcatgg tgggtatagc
      361 tcagctggtc agagcgccag gttgtggccc tggaggccgt gggttcaagt cccactattc
      421 accctttcat cgttgggtag tcgccaagcg gtaaggcaca ggactttgac tcctgcatgc
      481 gaaggttcga atccttccta cccagttaga tttgggctac tagctcaggc ggtagagcac
      541 tggactttta atccaggtgt cccgggttcg agtcccgggt agctcattct cgcggatgtg
      601 gcggaattgg cagacgcact agacttagga tctagcgcct ttggcgtggg ggttcgactc
      661 ccttcatccg catttaaaaa agcttcaaga cataggtctt gaagcttttt taatctttgg
      721 gaatgccgta aactgcaaga ttgctatttt tatatcggtt gtcatgagtc acgtcttttc
      781 caaaccagtc tggaggcgta aacttttccg aagccttttc tgattcaaat tctacttcca
      841 ctgtaagcag atcttctaaa tctccatgat aaacatctaa ttctgcaacc agattatttt
      901 ctaatggaat attatatcta cttttataga tggtattgga atcaactttt tgccaaaggc
      961 tttcaaattg ttcttttgtt aaaggaaatt ctacttcttc tctttttaaa tggcctttac
     1021 ttttaatagt aaggcaatag ttatcattac tttttcttaa tcggataata gggtcaattg
     1081 aaatataacc ttggatgatt tcttcctttt catatagatg aagattttca ggtaatcttt
     1141 tcactaaata tttacgttct atttccatga taaattcctc ccaagaaata ttaagagaac
     1201 agattacttt aattataatg tataattctc ttttaaaaaa gccaaaaaac aaaaaaataa
     1261 aaaagcataa ttttctaagg attaaacgat aaaatcttat ctagaaaaaa atgtttttat
     1321 tagttatttt tataaaaaaa tctggtttaa atttttcatc aaatgaatgt tttatagtaa
     1381 agtatcatta taataaagta ttaaagctaa attaatcact gataaaaagc tgtttcttaa
     1441 tattcataat taactaaata atatattaat aggttcaaaa atataaatat gaaatttttc
     1501 cttcatttta tataagatta tgataaaagt aatccattac gattcgagga taaatgaaaa
     1561 ataaattgca aattccgata aaaggtgtta taataatcct tgtttttaat caatcagcag
     1621 gtaagataaa ggaggacaat atatgttaaa gagattttgg gcaatgagct tagtgcttgt
     1681 aatggttttt gccattgctc ttacggcttg cagtaagtct gatgacaatc aaaccgttgg
     1741 agaagctcag caaaatacaa ataatgaaga tacaaaagaa gagtatcaag gagaaaaagt
     1801 attagattat tacttatcat ctgaacctca aaccttagac ccgcaacaaa tgtggggaca
     1861 accagatatc atggttgaaa atatgtttat ggaaggttta atgagatttg gtaaagacga
     1921 ttcttcccta gaaccaggag ttgctaaggg atatacttat gatgaagcaa gcaatactta
     1981 tacttttgaa cttcgtgaag atgcaaaatg ggaagatgga acaccaatta ctgcggatga
     2041 tttcttcttc gcatggagac tggctgtgga tacggcagca ccttatagct tcttaattac
     2101 agactatatt gaaggtgcag cggattacgc agcttatgat aaggcaagct ttttagctga
     2161 aaaagatgct gcctttgcta accttagtga ggaagaacag gctgctcgta ttgaagcgat
     2221 ggcagacgat gaattaaagc aatacaatgc taaaaaagat gaattatgga gtcaggtaaa
     2281 agcagtagca gaaggttcaa acatcaaaat aactctttct attccagcac catattttcc
     2341 aagcttaacc gcatttgcag tttatacacc tgcaagagaa gatttttaca aggaaaaatc
     2401 agctaaaaat aaatatggta ttgaggctga aggcttactg gctaatggac catggaaagt
     2461 tgaagaatgg gttcataaag attactttaa gctagtaaag aatgaaaatt attggaacaa
     2521 agacaacatt aatattgatg taattaactt gaaaatagtc aatgaagtag aaacaagaac
     2581 aaaccttctt aagacaggtg ctttagacgg ttcagccatt cagtcaaaag acgtaccgga
     2641 ttttgaagat atggcaactt tagaagaatt aaatttacaa ccaatgatta atcgctcaga
     2701 ctttagtgta ttctatattg actttaatca ttttgataat ccgattattc aaaataaaaa
     2761 catccgtaaa gctttaatgt atgctatgga cagaacttca tttgttgaaa aaattaatat
     2821 tggtgatgag cctgctttag catttgtgcc aaaagtattc ccaggacttg aaaagacatt
     2881 ccgagaagaa aatggcatgg aattatttaa agataacgat aaagaaaaag ccaaagaata
     2941 cctggaagct ggtcttaaag aattaggtct tactgaactt cctccattag atatgcttat
     3001 tggggattca gacattgacc aaaaagttgg tgagaagttc caggcagact ggaacgaaat
     3061 tggtattact gtaaatcttg ttccactgcc ttggggagaa agattaacaa gacttcaaaa
     3121 aggtgacttt gcaatgagtt cttccggttg gggaccagat tatcctgatg cgatgacttt
     3181 cttagaatta tttgaatccg taaatccaaa taataatgga caatataata atcctgaata
     3241 cgataaatta attaaggcag caaaaaatga aaaagatgca aaaaaacgta ttcaatattt
     3301 gtatgatgct gaaaagatgc ttatggaaga tgcagttatt gttccacaat actttagaaa
     3361 tgttcatttt acattcaaaa aatatgtaac aggtgttgta ataagaggcg taggagcaac
     3421 aaccgatttc tattgggcag atattgatat ggctgctaaa gctgcagaga aaaagtagtt
     3481 tgtttagtca gttaataata tatggaaagg tatatgtata tatacatata cctttcttaa
     3541 tataataaaa gaattattta ttcgattacc aggcttgaat ggttttagaa aatcataaat
     3601 gtgaggaggt taccatgtgc taaagtatat tctaaaacga cttctaatgg cccttgcaac
     3661 cttgtgggtt atatcgaccc tgacctttgt cttaatgcat gcgctgccta acgatccttt
     3721 tacggatcca aaattaaagc ctgaggtcag acaagcctta agagttaaat atggtttaga
     3781 caagcctcct tcagagcaat atgttatcta tatgaaaaat ttgcttaaag gggatttagg
     3841 aacatctatt aaatatcctg gccgttctgt ttcagatatt attgcagaaa gtttcccaaa
     3901 atcttttgct ttaggatggc gtgccctgat tattgctgta atttttggaa tgcttttagg
     3961 cattattgct gcattaaatc atcaaaagac tctggactat ttatcgattt ttattgcgat
     4021 aataggggta tctattcctt caattatttt aggcccgatt ttggcttatt tctttggagt
     4081 aaagctggga tggtttcctg ttacagttga taagagtgag atttctcttt ttctcccatc
     4141 cttaacatta gcgatgagtt ctttagcgac aatatccaga ttaatgagaa cgaccacatt
     4201 agaagtctta ggacaagatt atatcagtac tgcaaaagct aagggactat caaaaacaca
     4261 aattgtcctg aaacatgttt taagaaatgc agtgatgcct attattacta ttttaggacc
     4321 tttatttgca gccttaatca caggctctat tgttgttgaa aagatttttg cagttgctgg
     4381 tttaggagaa tattttgtat caacgataac agaacaggat tatcccatga taatgggtat
     4441 tacgattttt tatgctgcac tgattatttt atctatttta gtggtagata taatgtacgg
     4501 atttattgat ccaagactga gaatttccgg gaaaggaggg gaatagatga tgaatacgga
     4561 tatggataga aatgaattta aacatgttgg taaaaactta aaggcatccc aggaaataaa
     4621 gcgtcccagt ttatcttatt ggaaggatgc atacagaagg ttaaagaata ataaagttgc
     4681 aatgctttcc ttctgggtac tggttatttt aattattatg tctgctatag gtcctgtaat
     4741 tacaaagaat ttatacggtc atacctacga gagtcaaaat cttgcagagc aaaatcaaac
     4801 accaattatg acaaatgcaa gaagtatcgc cttattggcg aatgatgttt ttgaatatga
     4861 taaatattta tcaagttatc gtaaggataa gacaactttt aaaaacgtcc gacttaaatc
     4921 accgggtaaa attggattta aaataggaaa tgcagcagat gaatcctggc agggagacaa
     4981 aaaagaatac caattaatga ttgaagtaag cagttctgat acctggagca gtattattga
     5041 aaaattaaat aatgagggtc aaagacttct tcaggaagat cctgacttca gaggcattaa
     5101 attcgaaaaa aagggtagca atttagtgat agagaccgta ggtgaaaagt ggtttaataa
     5161 aacccattgg tttggtacag atgaatttgg aagagattta tttaccaggc tttgggaagg
     5221 tggaagaatt tctttcttta tcgcctttat ttccgtattc attgcatcta tttttggtat
     5281 tatttatggc ggactttcag gttatatagg aggaaaagta gacgaaatct taatgagaat
     5341 cgtcgaaatt ttaatgactg ttcccgacat gttatacaca atattattat taacggttat
     5401 ggatccagga attaaaccca ttagtattgt attaattgta acgggttgga tggggacagc
     5461 cagaatagtg cgtggagaag taatgcgttt aaagcattcc gaatatgttg ttgcagcaaa
     5521 aaccttaggt gctgatacga agaaaattgt ctttgaacat cttattccca atgccatggg
     5581 acccattatc gttaatatga caatgatgat tcctaaaatg atctttgctg aagcgttttt
     5641 aagctttatt ggtttagggg ttcctgtgcc ttatgcatcc tggggttctc ttgcaaacca
     5701 gggcgcaagg atattcaggc aattccctca tcagttattg attccggcta ttgctattag
     5761 cttaacaatg tttgctttta atttattagg tgacggatta agagatgctt tagaccccag
     5821 acttagaaaa taggaggtga gtcactgatg aaaaaaatcc tggaagttaa aaatctcgag
     5881 gtatcatttg atacttatgc aggcgaagta caggctgtaa gagatgtttc ttttgattta
     5941 tatgaaggag aaactctggc tattgttggc gaatctggtt caggtaaatc tgttacggca
     6001 aaatctttaa tacgtcttaa tcctgaacca cctgcaagat ataaagctgg agaaatcctt
     6061 ttgcacggtg aaaatatatt ggacaaaaaa gaaaaagaaa tgcagaaaat taggggaagt
     6121 aaaattggaa tgatttttca ggatccgatg acttccctta atcctactat gactgttggc
     6181 aaacaaatta tggaagcatt gatgaaacat caaaaagtag caaagtctga ggcaagaaaa
     6241 gttgctttag aaatgttaag aatggtgcaa atccctaatc ccgaaaaacg ctttgatgaa
     6301 tatcctcacc agttttctgg tggtatgaga caaagagtta tgatagcaat tgcattagta
     6361 tgcaatcctc aaatattaat tgccgatgag ccaacaaccg ccctggatgt aaccattcaa
     6421 gcccagatat tggatttgat gaagaattta caagaaaaaa ttaaaacttc aataatctta
     6481 attactcatg acttaggtgt tgtggctcat tctgcccaaa ggataattgt tatgtatgga
     6541 ggtaaaattg tagaaagcgg aacgacagat caaatttttt ataaatccag acatccttat
     6601 acctgggggt tattaaattc tgttcccaga ctggatatcc ataaagaaga tggtaggctg
     6661 gattctatag aaggtactcc tccggactta tttgcccctc ctcaaggctg tccatttgct
     6721 gaccgatgtg aatatgcaat ggaaatatgc agaaagaaaa tgccacctca gtttgaaaca
     6781 ggagaaaatc atcattctgc ttgctggtta aatcacgaat ttgctcctaa ggttgaaaat
     6841 cctactcaaa ttgccaaaaa gcttcaaaag gaggaggtgc agatgcgtga gcaatgatag
     6901 agaagttctt ataaaagtaa gaaacctgaa aaaatacttt aatattgata aaaatatcta
     6961 tcttaaggct gtagacgatg tttcctttga catttataaa ggagaaacat tggggcttgt
     7021 aggtgaatca ggctgtggca aatcaaccat tggaagaacg attttaaata tttataaagc
     7081 cacatctggt acagtagaat tcaaaggaaa aaacatctat gagcttaagg gaaaggaagc
     7141 caaggagttt acaagaaaag cccagatgat tttccaggac ccttatgctt cattaaatcc
     7201 gagaatgctt gtgtctaaca ttattggaga aggtattgat atacacaaat tattgactgg
     7261 acaggccaga atgaaccgta ttcatgaact gcttgaactg gtaggtttaa ataaagaaca
     7321 tgcaaaccgt tttccccatg aatttagtgg aggacaaaga caaagaatag gaattgcgag
     7381 ggccctggca gttaaccctg aatttattgt ttgtgatgag cccatatcgg cattggatgt
     7441 atctattcag gcacaagtgg ttaatctctt aaaagattta caggaaaaaa tgggcttgac
     7501 ctatttattt attgcccatg atttatccat ggtaagatat atttctgaca gagtagcggt
     7561 gatgtatctg ggttctatga tggaattggc caatagtgat gaactatatg aaaatcctct
     7621 tcatccttat actaaagccc tcttatcagc tattcccata ccagatccta aagtgcaaag
     7681 aaaaagagag aggattgtac tgcaagggga tgtgcctagt cctatcaatc cgccaaaagg
     7741 ttgtaaattt gcagaccgct gtcccatggc aacagagaaa tgtaaaaatg aaagacccaa
     7801 attagaagaa gtaaaagaaa atcattttgt tgcatgtcat tattggaaag aagctgaaaa
     7861 gagaatatga acaaaataaa aaagctatat aattatttca ataaataatt atatagcttt
     7921 tttattatgt caaaaaagat tataaatatt ttatcaacat attaaaaata ttgagataat
     7981 ataaaaagtt ataagactta aattgatata tgtatttttt gttttttagc aattttgctc
     8041 ggaaaaacct caatgcctta aaatatcatt cggctatgca aacaggtatg tatttaaaca
     8101 gttctttaag attatctcct gttatttcac agatttgggc ttgatttaaa gcctgctcaa
     8161 ttttatcagg attaatctga tagatatagg caatatcctc ttttgtataa atttttttat
     8221 atcctctttg catttgacgc ctaaggagtc cacagatata actgcttcct gtaaccataa
     8281 tgataaaaaa agaactggga ttaatagaat caatattata ttttgacatt aaatctttta
     8341 tttcttctac actaatatcc aacaaggcag atgtattttc aatattaagc tgttcgggag
     8401 tgattttgag aaaaagatcg atttcttgaa gttttttttc gatgtttttt tgatatacct
     8461 gaagaaatga atatttacag aagtgatatg aataagatga atccattatg agttcctcct
     8521 tgatgtagat aagaattgtg tcgattttta ttataacaaa ttcgcttgtt tatttcatgt
     8581 gtaatagttg gaataatcta ttgttttatg gaaatggtgc tatattccaa caaaagaact
     8641 aatatttttt aaaaaaatag ggataataat atcacggagg tgaaaaaaat ggccaataat
     8701 aatacgaata gtagaagaaa tgcagttcct caggcaagag cagcattaga caaatttaaa
     8761 ttcgaagtag ccaatgaaat cggagtacct ttaaagcaag gctataatgg agatttaact
     8821 tctgctcaag ctggttatgt aggcggatat atggttaaaa aaatgattga aagacaagaa
     8881 caacaaatgg ctggaaatac aaataatctt cagtaaaaaa ataaaaagat gacataatta
     8941 tttatgtcat ctttttttaa tgcttttggg atttaaaaga taacagtcat ttgccttata
     9001 atagattatt caaatataac gctttctaac atttgactta tttcttcttt tccttcttct
     9061 tccgtatatc cgacaacaat attaaatgca tatttatcca taatcttagt ataatatttt
     9121 tgagtataca cgaagctgcc aaagtcaata tttgattcta atacataaaa atcttctccg
     9181 cctaagtttt cagtgtaaat atctttaaaa ttataaggtg tttgacttgc actttctaac
     9241 tggtttctga tcgcttccag atattcactt tcattcttaa ctaatgaacc aactttttca
     9301 gcctgacaaa ttacatttgg gtttattttg ccaagttcac caatagggta tttgcatatc
     9361 atgaaaaagg ttaataattt ttgctctgca agatctaatg ctgctttttt agctgcattg
     9421 tctcctgcga tagcttcttt tccagcttcc atcagttcat taaccgtttc atcattttgt
     9481 atttcccagc cttcagggat attcatggat ataccaaaat aactgtttgt ataagtattt
     9541 ccctgaaatt cacctaaagt tagttctttt ttagaacaag ctgcagtgga taaacatact
     9601 aataatacca atacaaaaac taaagaattc tttactttat tcattatctc accctattcc
     9661 agaaattgtt gttgtgttac aatagatgac tgttatccaa tgaccaaatt ataatgttaa
     9721 taatagaaaa attcaattat ttcatttata gaaatttttg agataaaaat taattttttg
     9781 tatacaatta acataattaa a
//
LOCUS       DAOSUD010000074         9761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3810_length_9761_cov_22.777766, whole genome shotgun sequence.
ACCESSION   DAOSUD010000074 DAOSUD010000000
VERSION     DAOSUD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_9761_cov_22.777766"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..628
                     /locus_tag="PLG49_08815"
     CDS             281..628
                     /locus_tag="PLG49_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17451.1"
                     /translation="MKKTNYLICFLFFVILFCICFTGCNKNEVQQSNTDEISETNQLN
                     TNDKDSEAEQSNSEETNTENLEIDLADSESYFTIDPFEIEMDGKKLTLPFKWVEIEDT
                     VYTSKLDPLMFYN"
     gene            complement(744..2066)
                     /gene="eno"
                     /locus_tag="PLG49_08820"
     CDS             complement(744..2066)
                     /gene="eno"
                     /locus_tag="PLG49_08820"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ17452.1"
                     /translation="MKGYLEILDVFAREIMDSRGNPTVEVEVVVDAGGRQVVGRAAVP
                     SGASTGAFEAVELHDGGDRYVGKGVQNAVDNVNNIIAEEIIGMNALDQVEIDNRMIEL
                     DGTPNKSKLGANAILGVSLAVAKAAAEALDMPLYQYLGGFNAKVLPVPMMNILNGGKH
                     ADNTVDLQEFMIMPVGAKSFSEALRMCAEVYHNLKKVLSARGLSTAVGDEGGFAPDLA
                     TSEETIQVIIEAVEKAGYKPGEDIRIALDAAATELYDEETKTYRFPGESKMKGQEIVR
                     TSEEMVAYYEDLVNKFPIISIEDGLSEEDWEGWKLLTERLGKRIQLVGDDLFVTNTER
                     LAKGIELGVANSILIKVNQIGTLTETFNAIQMANRAGMTAVVSHRSGETEDATIADIA
                     VAVNAGQIKTGAPCRSDRVAKYNQLLRIEEELGDTAEYLGLKAWFNLK"
     gene            complement(2102..3646)
                     /gene="gpmI"
                     /locus_tag="PLG49_08825"
     CDS             complement(2102..3646)
                     /gene="gpmI"
                     /locus_tag="PLG49_08825"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOQ17453.1"
                     /translation="MMKKPTVLMILDGFGLNDRCEGNAICQAKTPVIDELMKKYPFVK
                     GYASGMDVGLPEGQMGNSEVGHLNIGAGRIVYQELTRITKSIQDGDFFKNPELLAAVN
                     NCKENNSSLHLFGLVSDGGVHSHNSHIYALLELAKREGLSKVYVHCFLDGRDTPPASG
                     KEYVAQLQEKIKEIGVGKIATVMGRYYAMDRDNRWERVKLAYDAMVLGEGNRCADPVE
                     AVQLSYDNEIYDEFVVPTVITASDEPVATIQARDSIICFNFRPDRVRQITRAFCDEDF
                     AGFERAKGFLPLKYVCFTEYDITIKNKEVAFKPTSLTKTLGEYLAEKGLKQLRLAETE
                     KYAHVTFFFNGGVEKPNEGEDRILVNSPKVATYDLKPEMSAIEVTDKLVEAILSNKYD
                     VIVINYANPDMVGHTGVLEAAIKAIETIDTCVGRAVDALLQVDGQMFICADHGNSEQL
                     VDYDNGEPFTAHTTNPVPFILVNAGEGIGLKEGGRLADIAPTLLDLMGLEKPEEMTGN
                     SLLVRN"
     gene            complement(3675..4427)
                     /gene="tpiA"
                     /locus_tag="PLG49_08830"
     CDS             complement(3675..4427)
                     /gene="tpiA"
                     /locus_tag="PLG49_08830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOQ17454.1"
                     /translation="MMRKKIIAGNWKMNKTPEEAQKLVEELKALVNTDKVDVVFCPPF
                     VCLPGVIEAVKGTHIAVGAQNIHFEESGAYTGEVSAEMLKNLGVKYVILGHSERREYF
                     HETDEIVNKKVLKALEHDLIPIICVGESLKQREQGITIDLVRLQTKIALKDVPADKAK
                     ELVIAYEPIWAIGTGKTATAEQAEEVCKAIREVVAEIYDQTVADAIRIQYGGSVNAGN
                     AKELFEMPNIDGGLVGGASLKIDFSKIVNYND"
     gene            complement(4440..5636)
                     /locus_tag="PLG49_08835"
     CDS             complement(4440..5636)
                     /locus_tag="PLG49_08835"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOQ17455.1"
                     /translation="MLNKKTVDDIQVKGKRVLVRCDFNVPLENGRITDENRLVAALPT
                     IKKLVNEGAKVILCSHLGKPKGEPKPEMSLAPVAKRLSELLGKEVVFAKDDNVVGENA
                     KNAVSEMKEGDIVLLENTRYRIEETKNIDDFSKELASLADVFVNDAFGTAHRAHCSNV
                     GVTKFIEGDCAVGYLMQKEIEFLGNAVNNPKRPFVAILGGAKVSDKINVINNLLDKVD
                     TLIIGGGMAYTFLKAMGYEVGKSLLEEDKVEFAKEMMEKAKAKGVNFLLPVDTVVAKE
                     FKNDAEQKVVERGGIEPDWEGLDIGPETRKLFADAVKDAKTVVWNGPMGVFEMPNFAK
                     GTMAVAEALAQTDAITIIGGGDSAAAVNILGYGDKMTHISTGGGASLEFLEGKELPGV
                     AAADDK"
     gene            complement(5750..6736)
                     /gene="gap"
                     /locus_tag="PLG49_08840"
     CDS             complement(5750..6736)
                     /gene="gap"
                     /locus_tag="PLG49_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOQ17456.1"
                     /translation="MAKIAINGFGRIGRNAFKIAIAKGMDIVAINDLTDAKTLAHLLK
                     YDTCFGKFDGTVEAKEGALVVNGKEIKVFAEKNPADLPWKELGVDIVIESTGIFRKKE
                     QAQLHIDAGAKKVIISAPGKGTKSIVMGVNESDYNPAEDDIVDNASCTTNCLAPVAKV
                     LHEKFGIKRGIMTTIHSYTNDQRILDLPHSDLRRARAAAESIIPTTTGAAEAVAKVIP
                     SLKGKLTGMAMRVPTPTVSVVDLTAELDKDVTVEEVNAALKAAADDKILGYTDEPLVS
                     IDFRQDSRSSIVDGLSTMVIDGNLVKVISWYDNEWGYSNRIVDLANYIAERL"
     gene            complement(6807..7844)
                     /locus_tag="PLG49_08845"
     CDS             complement(6807..7844)
                     /locus_tag="PLG49_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOQ17457.1"
                     /translation="MKNLSLTLRKIIPEGVLTLERRYEILRAISYSQPVGRRLLANQL
                     GISERIIRSETDFLKENSYINVTSLGMEITHDGYTILQGLENLIYELRGLTELEKQIS
                     ELLNIDKVIVVPGDSDKNEMVKKDIGKAAAQIFREILKPGNTIAITGGTTVAQMIQAF
                     QPLNNNNYEDVFVLPARGSIGNKVEYQSNTLASQLANKLGADYKLLNIPDHLSKKSLE
                     SISKEPEIKDTLQKVSNTDILIFGIGNALKMAKRRGLSETIIDFLRRKEAVAEAFGYY
                     FNLEGEIVYSSRSVGIQLDEVKNISYMIAVAGGSSKAEAIMAVKHLLRKGCIVMDEGA
                     ANEIVTYKTNR"
     gene            complement(7987..9636)
                     /locus_tag="PLG49_08850"
     CDS             complement(7987..9636)
                     /locus_tag="PLG49_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOQ17458.1"
                     /translation="MGEYIKVIFTLVGGLGLFIYGMQIMADGLQKSAGNKMKRLLEAL
                     TNKKLMGVLVGAVVTAIIQSSSATTVMVVGFVNAGLMNLSQAVGVIMGANIGTTVTSW
                     LVSSAEWAKFLNPSELAPLAIAVGVGMMIFAKRKFVKQIGEIIAGFGILFVGLDMMSG
                     AVKPFRESEIFKNAFLSLGRSPLLGIFAGLAVTAIIQSSSASIGILQSLARYSLVPRS
                     AAFYIIMGQNIGTCVTALFSSIGANKNGKRTAYIHLLFNVIGTMIFSIIAIVYFKYIN
                     QAVGESFITMTEISAVHTIFNVANTVILFPFSGMLVFLASQLVRGEDEEDESALRHLD
                     DRILETPSFAVESAIKEVIRLSKMAAENARLAMEALLEKSDEKAEQVYKREKSINALE
                     KAITPYLVKILNSSINERQNLIITSLFHITNDIERVGDHAENIAELAQFAIAENVQFS
                     DMAIEELKTLSEKSIGCYELAARAIEEDNPRLARQVEPLEEIVDKLEEEFRQTHIIRL
                     SQNKCSANSGVVFLDVISNLERISDHASNIAFAVLDQHKKA"
BASE COUNT         2937 a         2064 c         1436 g         3324 t
ORIGIN      
        1 ataaaaataa gcaaatactt ggcaattaat ataaaactgt tcttaaacca cagtttttta
       61 ttgatttaaa gttaattatg atttaaaatt atgcccatac ttaatttata aaaagaaagg
      121 agcggtcatc aataacagtt agcattatat aataatataa taactatttt atcaaccatc
      181 tatccttttt gcttgctaaa aaagatttta aaatgactca taaaatccag gaaaaactga
      241 tgcttaaaat tttataacaa aggaggaggc taatattaaa atgaaaaaga caaattactt
      301 aatttgtttc ttattctttg taatactatt ttgtatttgt tttactggtt gtaataaaaa
      361 tgaagtacag caatcaaata ctgatgaaat ttcagaaacc aatcaactga atactaatga
      421 taaagattct gaagcagaac agtcaaactc cgaagaaacg aatacagaaa atttagaaat
      481 tgacttagca gattcagaat cttattttac aatagatcct ttcgaaatag aaatggacgg
      541 aaaaaaatta acattgccat ttaagtgggt agaaatcgaa gatacggtat atacttccaa
      601 actagaccct ctaatgtttt ataattagat gctctatttg gaagtatttt ttattgtcta
      661 taaaaaaaga ttagaatgcc tggctaaatc aggcatccta atctatatac ataaaaaacg
      721 ctctatattg actaaccgct taattattta aggttaaacc atgcttttaa tccaagatat
      781 tctgctgtat cgcctaattc ttcttcgata cggagtaatt gattgtattt agcaacacgg
      841 tcagaacggc aaggagcacc agtcttaatt tggccagcat ttacagctac agcaatgtct
      901 gcaatcgttg catcttctgt ttctccggat ctgtgagaaa caactgctgt catgcctgct
      961 ctgttagcca tttgaatcgc attaaaggtt tcagttaatg taccaatttg atttactttg
     1021 attaaaatgg agtttgctac gcctaattca ataccttttg ctaatctttc agtgttggtt
     1081 acaaataagt cgtcaccaac caattgaatt ctcttgccta atctttcagt taataacttc
     1141 cagccttccc agtcttcttc acttaagcca tcttcaatag aaataattgg gaatttattt
     1201 actaaatctt catagtaagc aaccatttct tcagaggttc ttacgatttc ctgacctttc
     1261 attttgcttt cgcctgggaa acggtaagtt tttgtttctt catcatataa ttcagttgca
     1321 gcggcatcaa gcgcaatacg gatatcttct cctggtttgt atccagcttt ttcaaccgct
     1381 tcgataatta cctgaatggt ttcttcagat gttgctaagt caggagcaaa tcctccttca
     1441 tcaccaactg ctgtagataa acctctggca cttaatactt tcttaaggtt gtgatatact
     1501 tcagcacaca ttcttaaagc ttcgctgaag gattttgcac caactggcat aatcatgaat
     1561 tcttgtaagt ctactgtatt gtcagcatgt tttccaccat ttaagatgtt catcatagga
     1621 actggcaata cttttgcatt gaagccgcca aggtattggt ataaaggcat atctaatgct
     1681 tctgcagctg cttttgcaac agctaaagat acaccaagaa tagcgttagc acctaatttg
     1741 gatttgttag gtgttccgtc taattcaatc attctgttgt caatttcaac ttgatcaagc
     1801 gcattcattc cgataatttc ttcagcaata atgttgttaa cattatcaac tgcgttttgt
     1861 acacctttac ctacatatct gtcgccacca tcatgaagct caactgcttc aaaggcacca
     1921 gtagaagctc ctgaaggtac agctgctctt ccaaccactt gtcttccacc agcatctaca
     1981 actacttcta cttctacagt aggattgcct cttgaatcca taatttctct tgcaaatacg
     2041 tctaaaattt ctaagtatcc tttcatttta tttctccttt caacattagt atatatataa
     2101 tttagtttct aactaatagt gagttaccgg tcatttcttc cggtttttca agacccatta
     2161 aatcaagaag tgtaggcgct atatctgcaa gccttccacc ttcttttaat cctattcctt
     2221 ctcctgcatt aactagtata aagggaacag gattggttgt atgtgccgta aaaggctctc
     2281 cattgtcata atctactaac tgttctgaat taccatggtc tgcacaaata aacatctgac
     2341 catcaacttg taataaggca tcaaccgccc tgcctacgca agtatctatg gtttctatag
     2401 cttttatggc tgcttctaaa acgcctgtat gacctaccat gtccggattt gcatagttaa
     2461 ttacgataac atcatattta ttagaaagta tcgcctcaac cagtttatcg gttacttcaa
     2521 ttgcactcat ttccggcttt aaatcataag tcgcaacttt gggagaattt accagaattc
     2581 tgtcttctcc ttcattaggc ttttctacgc caccattaaa gaaaaaggta acatgggcat
     2641 acttttctgt ttcagccaga cgcaattgtt ttaatccttt ttcggccaaa tattcaccta
     2701 gagtcttggt taaacttgta ggcttaaaag caacctcttt attctttatg gtaatgtcat
     2761 attcagtaaa gcaaacatac ttaagaggca aaaaaccttt tgctctttcg aaacctgcaa
     2821 aatcttcatc acaaaatgct cttgtaattt gtcttactct gtcaggtctg aaattaaagc
     2881 agataattga gtctctggcc tgaatggtag ccacaggctc atcagaagct gtgatcactg
     2941 taggcacaac aaattcgtca taaatttcat tgtcatatga caattgaact gcttcaactg
     3001 ggtcagcgca tctgtttcct tcgcctaaaa ccatggcatc gtaagccagt ttaactcttt
     3061 cccagcggtt atccctgtcc atggcataat atcttcccat aacagttgct attttaccta
     3121 caccaatttc ctttattttt tcctgaagct gcgccacata ttcctttcct gaagccggag
     3181 gtgtatccct gccatctaag aaacagtgca catatacttt agacaagcct tctcttttag
     3241 ccagctccag caaggcatat atatgagaat tgtggctgtg tacccctcca tcagatacca
     3301 gtccgaataa atgaagggat gaattgtttt ctttgcagtt attcactgca gccagcaatt
     3361 caggattttt aaagaaatct ccatcttgta tagattttgt gattcttgtc agttcctgat
     3421 atacaatacg acctgctccg atgtttaaat gtccaacttc cgaattaccc atttggcctt
     3481 caggaagtcc tacatccatg ccactggcat aaccctttac aaaaggatac tttttcatca
     3541 attcgtctat tacaggggtt tttgcctggc aaatagcatt gccttcgcag cggtcattta
     3601 gtccaaaacc gtcaagtatc attaggactg ttggtttctt catcattata ataatactcc
     3661 ttctatcatc tttcttagtc gttatagttc acaatcttgc taaaatctat ttttaaactc
     3721 gctcctccga ctaagccacc gtcaatgtta ggcatttcaa aaagttcttt tgcatttcca
     3781 gcatttacgc tgccaccata ttgaatacgg atagcatctg ccactgtctg atcataaatt
     3841 tcagcaacca cctcacgtat tgccttacaa acttcttctg cttgctcagc agttgcagtc
     3901 ttacccgttc caatagccca tataggctca taagcaatca ctaattcttt tgctttatcg
     3961 gcaggaacat ctttcagagc aattttagtt tgtagacgga ctaaatctat tgtaatccct
     4021 tgttctcttt gctttaaaga ttctccgaca caaataatag gaatcaagtc atgttcaaga
     4081 gctttaagta cttttttatt aacaatttca tcggtttcat ggaagtactc tcttctttca
     4141 gaatgcccta aaatgacata ttttactcct aaatttttaa gcatttcagc agatacttca
     4201 ccagtatagg caccactttc ttcaaaatgg atattttgtg caccaacggc aatatgagta
     4261 ccttttactg cttcaatgac tccgggaaga catacaaaag gagggcaaaa gacaacatca
     4321 accttgtctg tattcactaa agcttttaat tcttccacca gtttttgtgc ttcttccgga
     4381 gttttattca ttttccagtt tcccgcaatg atttttttac gcataataac ctcctacact
     4441 tatttatcat ctgcagcagc tactccgggt agttctttac cttcaaggaa ttctaaagaa
     4501 gcaccgcctc cagtagaaat atgagtcatc ttatctccat aacctaatat atttacagca
     4561 gctgcagagt ctccaccacc aataatggta atcgcatcag tttgtgctaa tgcttctgca
     4621 acagccatgg taccttttgc aaagtttggc atttcaaaaa cgcccatagg tccgttccaa
     4681 acaacagttt ttgcatcttt tacagcatca gcaaatagtt ttctggtttc aggtccaata
     4741 tctaaacctt cccaatcagg ttcaatgccg cctctttcaa caactttttg ctctgcatca
     4801 tttttaaatt cttttgcaac aaccgtatca acagggagta aaaagtttac accctttgct
     4861 ttagcctttt ccatcatttc ttttgcaaat tcaactttat cctcttcaag aagggatttt
     4921 ccaacttcat atcccattgc tttaaggaaa gtatatgcca taccgccgcc aataattaag
     4981 gtgtctacct tatcaagaag attgttgatt acattaatct tatcagatac cttcgcgcca
     5041 cctaaaatcg ctacgaaagg tctttttgga ttatttacag cgttacctaa gaattcgatt
     5101 tccttttgca ttaaatatcc tacagcacaa tcgccttcga taaacttagt aacacctaca
     5161 ttggagcagt gtgctctgtg agctgttcca aaagcatcgt taacaaatac atccgcaagg
     5221 cttgctaatt ctttactaaa atcatcgatg tttttggttt cttctatacg atatcttgta
     5281 ttttcaagaa gaacgatatc gccttctttc atttcgctaa ctgcattttt agcgttttct
     5341 ccaacaacat tatcatcttt tgcaaataca acttcttttc ccaataattc acttaatctt
     5401 ttagcaacag gtgctaatga catttcaggt ttgggttctc ccttaggttt tcctaaatga
     5461 gaacataaga tgacctttgc accttcatta actaatttct taattgttgg aagagctgct
     5521 accagtctgt tttcatccgt aattctacca ttttcaagtg gaacattaaa atcacagcga
     5581 actaacactc ttttaccttt aacctgaata tcgtctacag tctttttatt taacatttta
     5641 ttaatattcc ctcctcggca aattcagttg atatggataa ttaaaattga aataaggtcc
     5701 ggtcctatag atactactac aaaaccggac ctaaaggtct tagcaattat taaagacgtt
     5761 cagcaatata atttgctaag tcaacgattc tgttagaata tccccattca ttgtcgtacc
     5821 aggaaataac tttaactaag ttgccatcaa tcaccattgt ggataaacca tctacgatag
     5881 aagaacggga atcttgtctg aagtcaatag atactaatgg ttcatctgtg tatcctaaaa
     5941 tcttgtcatc tgcagcagct ttaagagcag catttacttc ttctactgtt acgtctttat
     6001 ctaattctgc tgttaagtca actacagaaa cagtaggtgt aggaacgcgc attgccattc
     6061 ctgttaattt tcctttaaga gaaggaataa cttttgcaac tgcttcagca gctcctgtag
     6121 ttgtaggaat aatagattcc gctgccgcac gtgctcttct taaatcactg tgtggtaagt
     6181 ctaaaattct ttggtcattg gtataagagt gaatagttgt cataattcct ctcttaatac
     6241 cgaatttttc atgaagcact tttgcaacag gtgctaaaca gttggttgta caggatgcat
     6301 tgtcaacaat gtcatcttct gcaggattgt aatcactttc attaacgccc ataacgattg
     6361 attttgttcc ttttccagga gcagaaataa taactttctt tgcacctgca tctatatgta
     6421 attgtgcttg ttctttcttt ctgaaaatac ctgtagattc aattacaata tcaacgccaa
     6481 gttctttcca tggaagatca gcaggattct tttcagcaaa aactttgatt tctttaccgt
     6541 ttacaactaa agcaccttct ttagcttcaa cagttccatc aaattttcca aagcaagtat
     6601 catattttaa taaatgagct aaagtctttg catcagttaa atcattaatc gctacaatat
     6661 ccattccttt tgcgattgct attttgaaag catttcttcc aattcgtcca aaaccattaa
     6721 ttgcaatttt tgccattttt cattcctcct ataaaattta atttattata attgtaagac
     6781 ataaaaatat gcttacaata ttcagcttaa cgattcgttt tatatgttac tatttcattg
     6841 gcagctcctt catccataac aatacagcct tttcttagaa gatgttttac tgccataatt
     6901 gcttctgcct ttgaggaacc tccagcaacg gcaatcatat aagaaatatt tttaacttca
     6961 tctaattgaa tccccacaga acgggaactg tatacaattt cgccctcaag attaaaataa
     7021 tatccaaaag cttcagctac tgcttcttta cgtcttaaaa aatctataat tgtttctgac
     7081 agccctcttc ttttagccat cttaagagca ttgcctatac caaaaatcaa aatatctgtg
     7141 ttagatactt tttgaagcgt gtcttttatt tccggttctt tgctgatgct ctccagggac
     7201 tttttactta aatgatcagg aatatttaaa agcttataat cagcacccaa tttattagcc
     7261 agttgagaag ctaaagtatt ggactggtat tcaactttat taccaatact gcctctggct
     7321 ggcaacacaa aaacatcttc ataattatta ttatttaaag gttgaaaagc ctgaatcatt
     7381 tgggcaactg ttgtccctcc ggtaatggca atagtattgc ctggttttaa gatttccctg
     7441 aaaatctgtg cagcagcttt tccgatatct tttttgacca tttcgttttt atcggaatct
     7501 ccgggaacaa caatgacttt atcgatattt aatagttcag atatttgttt ttcaagctct
     7561 gtaagacctc ttaattcata tataaggttt tccaacccct gaagaatagt atatccgtca
     7621 tgagtaattt ccatgcctaa agaagtaaca tttatatacg aattttcttt taaaaaatct
     7681 gtttcacttc ttataatcct ctctgatatg cctaactgat tggctaacag tcttctgcca
     7741 acaggctggg aataactgat ggcccttaat atttcatatc ttctttctaa agtcaaaacc
     7801 ccttcaggaa taatctttcg caatgtaaga gaaagatttt tcattataat ctctcctcgg
     7861 gactattttt gtcccgcgcg acaattttcg tcccagtact cataaaaaaa tttctcattg
     7921 tcgcttctat tgtagcgaaa acgagataaa atatcaagaa aaaaatagaa aaaaactatg
     7981 aattttttaa gcttttttat gctgatccag aacggcaaaa gcaatattgg aagcatggtc
     8041 agaaattctt tctaaattgc tgatgacatc caaaaacaca acgcctgaat ttgcactgca
     8101 tttattttgg gaaagtctta taatatgggt ctgtctgaat tcttcttcca acttgtccac
     8161 gatttcttct aaaggctcaa cttgtctggc cagtctagga ttatcttctt caatggctct
     8221 ggccgccagt tcataacagc ctatactctt ctcagacaga gtttttaatt cttctatggc
     8281 catatctgaa aactgcacat tttcagcaat tgcaaattgc gccagttcag caatattttc
     8341 tgcatgatcc ccgactcttt ctatatcatt cgtaatatga aataagcttg taatgattaa
     8401 attttgccgt tcattaatcg atgaatttaa tattttaacc agataaggag taatagcttt
     8461 ttctaaggca ttaatacttt tttctctttt ataaacctgc tcggctttct catcactttt
     8521 ttctaataaa gcctccatgg ctaacctggc attttctgct gccattttac ttaaacgtat
     8581 cacttcttta atagcacttt ccacagcaaa ggaaggcgtt tctaatattc tgtcatctaa
     8641 atgtctgagc gcactttcgt cttcttcatc ttcaccccta accaattgac tggctaaaaa
     8701 aaccagcatt ccggaaaaag ggaataaaat aaccgtattg gcaacattaa aaatcgtatg
     8761 tacagcactg atttctgtca ttgtgatgaa agattctcca acagcctggt tgatatattt
     8821 aaaatacaca atagctataa tcgaaaaaat catggtaccg attacattaa aaagcaagtg
     8881 aatatatgcc gttctttttc cgtttttatt ggcaccaata cttgaaaata aagccgttac
     8941 acaagttcca atattttgtc ccatgatgat ataaaaagca gcactcctgg gtactaagga
     9001 ataccttgct aatgattgta aaataccaat ggaagctgaa ctgctctgaa taatagctgt
     9061 tactgctaaa cctgcaaaaa tacctaacaa aggacttctc cctaagctta gaaatgcatt
     9121 tttaaatatt tcagattccc tgaaaggttt tactgcacca ctcatcatgt ctaaaccaac
     9181 aaataagatc ccaaaacctg caataatttc tccaatttgt ttgacaaatt tccttttggc
     9241 aaaaatcatc atacctacac caacagctat agcaagaggg gctaattctg aaggatttaa
     9301 aaattttgcc cattctgcac ttgaaacaag ccagctggta acggttgtcc cgatatttgc
     9361 acccataata acacctacag cttgtgacaa gttcattaag cctgcattta caaaaccaac
     9421 gaccataacg gttgttgctg aactgctttg aataatggcc gtaacaacgg caccaaccaa
     9481 tacgcccatt aatttcttat ttgtaagagc ttctaacagt cttttcattt tatttccagc
     9541 agacttttgt aagccatctg ccataatttg cataccataa ataaaaagtc ccagtccccc
     9601 gacaagggta aaaattacct tgatgtactc tcccatcttt tacctcccaa tttattatta
     9661 cttcctgaag tattctatca tggagccaaa aagctaagca agcctttata atttattaga
     9721 gtttatttat tatttatata caaaaatatt tatattatta a
//
LOCUS       DAOSUD010000075         9727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3819_length_9727_cov_24.946030, whole genome shotgun sequence.
ACCESSION   DAOSUD010000075 DAOSUD010000000
VERSION     DAOSUD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_9727_cov_24.946030"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1754
                     /locus_tag="PLG49_08855"
     CDS             372..1754
                     /locus_tag="PLG49_08855"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ17459.1"
                     /translation="MEKTMDQIVALAKSRGFIYPGSEIYGGLSNTWDYGPLGVELKNN
                     VKRAWWKKFVQESPYNVGVDCAILMNTRVWEASGHVGGFNDPLMDCKECKERFRADQL
                     IESYLKENGIELSVDGWDNAKMKAYIDENQIKCPSCGAHNFTDIRQFNLMFKTYQGVT
                     EDSKNLVYLRPETAQGIFVNFKNVQRTTRKKIPFGIAQIGKSFRNEITPGNFIFRTRE
                     FEQMELEFFCEPGTDLEWFNYWKEFCKNWLLSLGMKEEHIRLRDHSKEELSHYSNATT
                     DIEFLFPFGWGELWGIADRTDFDLKQHQEYSGEDMNYFDPNTNQKYIPYCVEPSVGVD
                     RVTLAFLCEAYEEEELEDGDKRTVLRLHPALAPVKAAVLPLSKKLSDSAYKVYEKLLK
                     LFNVDFDDSGSIGKRYRRQDEIGTPFCITFDFDSLEDNAVTVRDRDSMQQERIKIDDL
                     KNYLKNKMDF"
     gene            1924..4551
                     /gene="ppdK"
                     /locus_tag="PLG49_08860"
     CDS             1924..4551
                     /gene="ppdK"
                     /locus_tag="PLG49_08860"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ17460.1"
                     /translation="MSKKYVYMFSEGDASMRNLLGGKGANLAEMTKMGLPIPQGFTVT
                     TEACNKYYEDGEVIAGEVVEQIKASLAKLEEIAGKKFGDSKNPLLVSVRSGARVSMPG
                     MMDTILNLGLNDISVEGIAESTQNPRFAYDSYRRFIQMFSDVVMEIAKSKFEKVLDEM
                     KESKGVKYDSELTAEDMKNVVAKFKEIYKAEKGEDFPQDPYAQLMAAVRAVFRSWNNP
                     RAIVYRRMNDIPSNWGTAVNVQMMVFGNMGETSGTGVAFTRNPATGDKEIFGEYLINA
                     QGEDVVAGIRTPKSIKTLEEDMPEVYKQFMDIANTLEKHYKDMQDMEFTIENGKLFFL
                     QTRSGKRTAKAALKIAVDMVDEGLINEEEALLKVEPKQLDQLLHPTFDADALKKAKPL
                     GKGLPASPGAAAGKVYFTAEDAKNAKEAGERVILVRLETSPEDIEGMVAAEGILTVRG
                     GMTSHAAVVARGMGTCCVCGLGEIKINEEEKYFTLAGQTFHEGDYISLDGSTGNVYGE
                     DIPTVKPEITGNFARFMEWADKVRKLKVRTNADTPKDAKTAVEFGAEGIGLTRTEHMF
                     FEEDRIPKVRKMILAKTEEERRKALDALLPLQKGDFIGIFEAMEGRPVTIRLLDPPLH
                     EFLPHEDDDIRELAKDMGITFEELKATVVSLHEFNPMMGHRGCRLAVSYPEIAEMQTR
                     AIIEAAIEVKKNKGYDIVPEIMIPLVGEVKELKFVKSVVVKTADEVIKEAGVNLTYKV
                     GTMIEIPRAAVTADQIAEGAEFFSFGTNDLTQMTFGFSRDDAGKFLEDYYEKQIYDFD
                     PFAKLDQTGVGELVKIATEKGRSTRPDIKLGICGEHGGDPSSVEFCHNIGLDYVSCSP
                     YRVPIARLAAAQAQVKNPR"
     gene            4719..5729
                     /locus_tag="PLG49_08865"
     CDS             4719..5729
                     /locus_tag="PLG49_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOQ17461.1"
                     /translation="MLIREVQEKKEYDLLSPYASKAAETKGRLFEEEKCSIRTDFQRD
                     RDRIIHCKSFRRLKHKTQVFITPEGDHYRTRLTHTLEVAQIARTIARALSLNEDLTEA
                     IALGHDLGHTPFGHAGEEVLNEVCPYGFKHYEQSIRVVNFVEKNGKGLNLTWEVRDGI
                     LNHPSKGSPGTLEGKIVQISDKIAYINHDIDDAIRGKVLTEEDLPKDCIEVLGKTFTE
                     RIDTMIHDIVKNSINQPYIKMSPDVEESMKKLRKFMFSHVYIGSKAKEQEKKAQHIVK
                     ELYYYFMNHPNEIPKDFYAIYEGKDEAQERIVCDYIAGMTDRYAINKYCEIYVPSSWS
                     IY"
     gene            5739..6197
                     /locus_tag="PLG49_08870"
     CDS             5739..6197
                     /locus_tag="PLG49_08870"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HOQ17462.1"
                     /translation="MYKTRIIIDGDSCPVLKIIERVSQKYQIKAILFCSYCHLPNEKY
                     HMEYRIVDSLPQAADMAIINFIDKEDIVVTQDYGLAALVLAKGAFAISTNGYIYSNEN
                     MDMLLYRRHLSSEIRKMKGRIKGPPKRNETDDFEFEENLIKLIEQKKYVQ"
     gene            6322..8127
                     /gene="dnaG"
                     /locus_tag="PLG49_08875"
     CDS             6322..8127
                     /gene="dnaG"
                     /locus_tag="PLG49_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ17463.1"
                     /translation="MYYSEEIIEEVLSRNDLVDVVSEYIRLKKQGSSYFGLCPFHNED
                     TPSFSVSPDKQMYYCFGCQAGGNVFTFIMQMENYSFAETVKYLADRANITLPQPEYSA
                     EVKKALKEKEILRNIHKEAAKYFYYNLQSERGKVALEYLQGRKVEEKIRKLFGLGYAN
                     ADYDDLYKYLLYKGYDEEFIAKSGLVIPRKTGKGYIDRFRNRVMFPIFDVHNNIIGFG
                     GRVLDDSLPKYMNSPETQLFDKSRNLYGLNYARTARENNILIVEGYMDVISLYQAGFK
                     NVVASLGTAFTYQHAALLKRYTNEVVLVYDNDAAGIKAALRAIPILSSAGLKIKVLQI
                     PGEKDPDDFIKEKGPEAFRDLIMKALSSVMFQVNVVKAKYNLENLEEKIQFTKEVANI
                     LAKLNSSIEIEVYSEQIAEETGVSKQSILEEIKKLRAIRQKENRKINKSSFSTNESEN
                     NLKSSSQQKADEKDGLYKAQLNLLRLISQNETIYQKIKDYLSFSEFIDPVLSKAAKLI
                     YESYGEGRSIEAAKIINHFENLQEQNQVATIFNEEKSSVKEYDVEKAINDQIKLIKRA
                     NIDLKSRATTDIYEIQALIEQKKQLEKLYINIISG"
     gene            8161..9237
                     /gene="rpoD"
                     /locus_tag="PLG49_08880"
     CDS             8161..9237
                     /gene="rpoD"
                     /locus_tag="PLG49_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOQ17464.1"
                     /translation="MKTSEQNKFLTHLKELVAIAKQKKGVLEYKEIMDHFADMELEPE
                     KIEKIYEYLERQNIDVIGIIEEDEGVIEDDLDLSLPEGISIDDLVRMYLKEIGKVPLL
                     TAEEEIELAIRMENGDEEAKRKLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIK
                     AVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQ
                     ELGREPTPEEIAKEMDMPVSKVREIIKIAQEPVSLETPIGEEEDSHLGDFIPDEDVPV
                     PSEAAAYTLLKEQLMDVLDTLTDREQKVLRLRFGLDDGRARTLEEVGKVFNVTRERIR
                     QIEAKALRKLRHPSRSKKLKDYLE"
     gene            9325..>9727
                     /locus_tag="PLG49_08885"
     CDS             9325..>9727
                     /locus_tag="PLG49_08885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ17465.1"
                     /translation="MILSKRLSAIANEVPHYASIADIGTDHAYLPIYLYKHNKIKKAI
                     ATDINKGPLEKAKNNIMSHQADRIIETRLGNGLEVLKENEVDTIILAGMGGMLIIDIL
                     KKRPEIVENIDRLIIQPQLDQNEVRKYLHSIN"
BASE COUNT         3501 a         1360 c         2004 g         2862 t
ORIGIN      
        1 ttcagacttt agaatttatc aagacaaatt taatggattt gcagtaaatc attattgaca
       61 aatttcatgt aatttgctag attaatgata atctcataag aatttgcgat gacaaagagg
      121 agtagctatt tgaattctta tagcgagctg gtgagggtgt aagaccagta gaattcgtta
      181 gcgaagggcg ctttggagca atatttttta aagaggatgc cttgcatcaa tgagggtgga
      241 accgcggaag agtattcttt cgtcccttgt atagatgtac aggttttttt attgctcaaa
      301 ataaatataa tattaatgtt tggattaaaa atgcgttgag tcgttttttt aagataagga
      361 ggctattcat catggaaaaa acaatggacc aaattgttgc attggcaaaa tcaagaggtt
      421 ttatctatcc gggttccgaa atatatggag gattatcgaa tacctgggat tatggcccat
      481 taggcgtaga attaaagaat aatgtaaaaa gagcctggtg gaaaaaattt gtgcaggaaa
      541 gtccatataa tgtgggtgtt gattgcgcaa ttctgatgaa tacgagagtt tgggaagctt
      601 caggacatgt aggaggtttt aatgaccccc tgatggactg taaagagtgt aaagagcgct
      661 tcagggctga tcaacttatt gaaagttatt taaaagaaaa tggtatagag ctttctgttg
      721 atggttggga taatgctaaa atgaaagcat atatagacga aaatcaaatt aaatgcccaa
      781 gttgcggtgc tcataacttt accgacatca gacaatttaa cctgatgttt aaaacttacc
      841 agggagttac agaggattct aaaaacttag tgtatctccg tcccgaaaca gcacaaggta
      901 tttttgtgaa ttttaaaaat gtccaaagaa ctaccagaaa aaagattccc tttggaattg
      961 cccaaatcgg gaaatctttc agaaatgaaa tcactcctgg aaacttcatt ttccgtacca
     1021 gggaatttga acaaatggaa ttagaatttt tctgtgaacc aggtacggat ttagaatggt
     1081 ttaattattg gaaagaattc tgtaaaaatt ggcttttatc tttgggaatg aaagaagaac
     1141 atattcgcct gagagatcat tctaaggaag aattatccca ctatagtaat gcaacaacgg
     1201 atattgaatt tctatttcct tttggttggg gcgaattatg gggtatagct gacagaactg
     1261 attttgactt aaaacagcat caggagtatt ccggagagga catgaattat ttcgatccaa
     1321 ataccaatca aaagtatatt ccttattgtg ttgaaccttc cgtaggtgta gacagagtta
     1381 cccttgcatt tttatgtgag gcttacgaag aagaagaatt agaagatgga gataaaagaa
     1441 ctgtattaag actccatcct gctttggcac ccgttaaggc tgcagtttta cctctttcta
     1501 aaaaactttc tgattctgcg tataaagttt atgaaaaatt gttaaaacta tttaatgtgg
     1561 attttgatga cagcggcagc attggaaaac gttatagaag acaagacgaa ataggaacac
     1621 cattttgtat tacttttgat tttgattctt tagaagataa tgctgttacg gttagagaca
     1681 gggatagtat gcagcaagaa agaattaaaa tcgatgattt gaaaaactac ttaaaaaata
     1741 aaatggactt ttaaatctta aaaaaaaatg taatttcccc tagtcttttt ggggttttat
     1801 gatataataa ttaacggttg aatagacaaa acgtgacaag ttacataaac aagacatttt
     1861 tttggcggaa aatggttttg tttattctac aaatgaatat taagatacag gaggttaagg
     1921 ataatgagca aaaagtatgt ttatatgttc agtgaaggcg acgcatccat gagaaattta
     1981 cttggaggta agggtgcaaa tcttgctgaa atgacaaaaa tgggactccc aattccacaa
     2041 ggttttactg ttacaacaga agcttgtaat aaatattatg aagatggtga agtaatcgct
     2101 ggtgaagtag ttgaacaaat taaagcttca ttagctaaat tagaagaaat agccggaaag
     2161 aaatttggag attctaaaaa tcctttatta gtatccgttc gttctggtgc aagagtttct
     2221 atgcctggta tgatggatac aatcttaaac ttaggattaa atgacatatc tgtagaagga
     2281 attgcagagt ccactcaaaa tccacgtttt gcgtacgact cttacagaag atttatccaa
     2341 atgttctctg acgttgttat ggaaattgct aaatctaaat tcgaaaaagt tcttgatgaa
     2401 atgaaagaaa gcaaaggtgt aaaatatgat tctgaattaa cagcagaaga catgaaaaat
     2461 gttgttgcta agtttaaaga aatttacaaa gcagaaaaag gggaagattt cccacaagat
     2521 ccatatgcac aattaatggc agctgtcaga gcggtattcc gttcatggaa taacccaaga
     2581 gcaattgtgt acagaagaat gaatgacata ccaagtaatt ggggaactgc tgttaacgtt
     2641 cagatgatgg tatttggtaa tatgggagaa acatctggta caggtgtagc atttaccaga
     2701 aatcctgcaa caggtgacaa agaaatcttt ggtgaatact taattaatgc tcagggtgaa
     2761 gacgttgttg caggtatccg tactccaaaa tctatcaaga ctttagaaga agatatgcct
     2821 gaagtttaca aacaatttat ggacattgcc aatacattag aaaagcatta taaagacatg
     2881 caagatatgg aatttaccat tgaaaacggt aaattgttct tccttcaaac ccgtagcggt
     2941 aaaagaactg caaaagcagc attaaaaatt gctgttgaca tggtagatga aggcttaatt
     3001 aatgaagaag aagctttact taaagtagaa cccaagcaat tagaccaatt gcttcaccca
     3061 acttttgatg cggatgcatt aaagaaagca aaaccattag gaaaaggtct tcctgcatct
     3121 cctggtgctg ctgcaggtaa agtatacttt actgcagaag atgctaagaa cgcaaaagaa
     3181 gcaggagaaa gagttatctt agttcgtctt gaaacatctc ctgaagatat cgaaggaatg
     3241 gtggctgctg aaggtatttt aaccgtacgc ggtggaatga cttctcacgc tgctgtagta
     3301 gcaagaggaa tgggaacttg ctgtgtatgc ggacttggtg aaattaagat taatgaagag
     3361 gaaaaatact ttacattagc tggtcaaacc ttccatgaag gagattatat ctctttagat
     3421 ggttctactg gtaatgttta tggtgaagat atacctacag taaaacctga aattacagga
     3481 aactttgcaa gatttatgga atgggctgac aaagtaagaa aacttaaagt aagaaccaat
     3541 gcagatacac caaaagatgc taaaacagca gtagaatttg gtgctgaagg aattggatta
     3601 acccgtactg agcatatgtt ctttgaagaa gaccgtattc caaaagtacg taaaatgatt
     3661 cttgctaaga ctgaagaaga aagaagaaaa gcattagatg cattacttcc attacaaaaa
     3721 ggtgacttta ttggaatctt tgaagcaatg gaaggacgtc ctgtaactat ccgtcttctt
     3781 gacccaccac ttcatgaatt cttgcctcat gaagatgatg atatcagaga attagctaaa
     3841 gatatgggca ttacttttga agaattaaaa gcaacggttg tatctttaca tgaatttaac
     3901 ccaatgatgg gtcatagagg atgccgtttg gctgtatctt atcctgaaat tgcagaaatg
     3961 caaacaagag cgattattga agcggcaatt gaagttaaga agaataaagg ctatgatatc
     4021 gttcctgaaa ttatgattcc attggtagga gaagttaaag aattaaaatt tgttaagtct
     4081 gtagttgtaa aaactgctga tgaagtaatc aaagaagcag gcgttaactt aacatataaa
     4141 gtaggtacca tgattgaaat cccaagagct gcagtgactg cagaccaaat tgccgaagga
     4201 gcagaattct tctccttcgg tacaaacgac ttaactcaaa tgacctttgg attctcaaga
     4261 gacgatgctg gtaaattctt agaagactat tatgaaaaac aaatttatga ctttgatcca
     4321 tttgcaaaat tagaccaaac tggtgttggt gaattagtaa aaattgcaac tgaaaaaggt
     4381 cgttcaacca gaccagatat taaacttgga atttgtggag aacatggtgg agatccttca
     4441 tccgttgaat tctgccataa cattggttta gattatgtat cctgttctcc atacagagta
     4501 ccaattgcaa gattagctgc agcacaagct caagtaaaaa atcccagata ataatgtttc
     4561 aataaacaat aaagggtatt gtctttaaag tattaaaggc aatacccttt ttaattaagg
     4621 aaatttatta ttttaggtgt tttgaaggat tttgaaaaaa gatgtagtat atattacata
     4681 tggagatttt ttatgattaa cattaggtgg tgacttcttt gctgataaga gaagttcagg
     4741 aaaaaaaaga atatgattta ttaagtcctt atgcctcaaa agcggcagag acgaaaggca
     4801 gacttttcga agaagaaaaa tgcagtattc gaacagattt tcaaagagac agggatagaa
     4861 ttattcattg caaatccttc agaaggttaa aacataaaac acaagtattt attactccgg
     4921 aaggggacca ttaccgtaca agacttactc atactttaga ggtggcgcaa attgcaagga
     4981 ctattgcaag agctttgtca ttaaatgaag acttgactga agcgattgct ttaggtcatg
     5041 atcttggaca tacgcctttt gggcacgccg gtgaagaagt tcttaatgag gtctgccctt
     5101 atggctttaa gcattatgag caaagtatca gggtagtcaa ttttgttgaa aagaatggaa
     5161 aaggtttaaa tcttacctgg gaagttcgcg atggaatact caatcatcct tcaaaaggca
     5221 gtccaggtac tttagaagga aagattgttc aaatttctga caagattgct tatattaatc
     5281 atgatattga tgatgcgata cggggtaaag ttttaacgga ggaagatctc cctaaagatt
     5341 gtattgaagt tttaggaaag acttttacgg aaagaattga taccatgatt catgatattg
     5401 ttaagaatag tatcaaccaa ccctatatta aaatgagtcc tgatgttgaa gaaagcatga
     5461 aaaaattaag aaagtttatg ttttctcatg tatatatcgg ttctaaagcg aaagaacaag
     5521 aaaagaaagc tcaacatatt gtaaaagaac tttattatta ttttatgaat catcccaatg
     5581 aaattccaaa agatttttat gcaatttatg aggggaaaga cgaagcacaa gaaaggattg
     5641 tatgtgatta tattgcgggt atgacagacc gttacgcaat caataaatat tgcgaaatct
     5701 atgttccttc atcctggagc atctattagg aggcagcaat gtataagacc cggattatta
     5761 ttgatggtga cagttgtcca gtgcttaaaa ttattgaaag agtatctcaa aaatatcaga
     5821 taaaggccat tttgttttgt agttattgcc atcttccaaa tgaaaaatat catatggaat
     5881 accggattgt ggattccctg cctcaggcag cagatatggc aattattaat tttattgata
     5941 aagaagatat tgtcgtaacc caagattatg ggttagcagc tttggtttta gcaaaaggtg
     6001 cttttgccat atctacgaat gggtatatat attctaatga aaatatggat atgctattgt
     6061 acaggaggca tttgtcgagt gaaatcagga agatgaaggg tcgtataaaa gggcctccta
     6121 aaagaaatga aacagatgat tttgaatttg aagagaattt aataaaattg attgaacaaa
     6181 agaaatatgt tcaataaatt tttgctaggc gtaaattttt tatgtagttt atacaaattt
     6241 ttagaaaata tatggtttaa aaaaggattt aaagaaaaga tgtcgaaaat tatatgttag
     6301 catgaaagtt aggggagcaa aatgtattat tcagaagaga ttattgaaga agttcttagc
     6361 cggaatgact tagttgatgt cgtttctgaa tatataaggc tcaaaaagca aggcagttcc
     6421 tattttggct tatgtccttt tcataatgag gatacgcctt ctttttcagt tagtcctgat
     6481 aagcaaatgt attactgctt tggttgtcag gcaggaggaa atgtttttac ttttatcatg
     6541 cagatggaaa attacagctt tgcagaaact gtaaagtatt tagctgacag agcaaatatt
     6601 actttgcccc agccagagta ttctgcagaa gttaaaaaag ccttaaaaga aaaagaaatt
     6661 cttcgtaata ttcataaaga agcggctaaa tacttttatt ataatctaca atccgaaaga
     6721 ggcaaggttg ctcttgaata tttacaaggc agaaaagtcg aagaaaaaat acgtaaatta
     6781 tttggtttag gttatgccaa tgcagattat gatgatttat acaaatattt attatacaaa
     6841 ggatatgatg aagaattcat tgccaaaagc ggcttagtca tcccaaggaa aactggcaaa
     6901 ggttatattg accgttttag aaatagagtc atgtttccta tttttgatgt tcataataat
     6961 attattggtt tcggcggaag agtattagat gatagtcttc caaaatacat gaactcgcct
     7021 gagacacaat tatttgataa aagcaggaat ttatatggtt taaattatgc cagaacggct
     7081 cgtgaaaata atattttgat tgttgaaggt tatatggatg tgatttcttt ataccaggca
     7141 ggttttaaga atgttgtagc atccctggga acagcattta cttaccagca tgctgcctta
     7201 ttgaagcgtt ataccaatga agtggttttg gtttatgata atgatgctgc aggaatcaaa
     7261 gccgccctaa gggcaattcc cattttatct tctgcgggct taaagataaa ggtactccaa
     7321 attccgggtg agaaagaccc tgatgatttt attaaagaaa agggcccaga agcattccgg
     7381 gatttaatta tgaaagcttt aagttctgtg atgtttcagg tgaatgtcgt aaaagcaaaa
     7441 tataatcttg aaaatcttga agaaaaaata cagtttacaa aggaagttgc taatatttta
     7501 gcaaaattaa acagttctat tgagatagaa gtttattctg aacaaattgc agaagaaacc
     7561 ggcgtttcaa agcaatctat ccttgaagaa attaaaaaac taagagcaat tcgtcaaaaa
     7621 gaaaatagaa aaattaataa gtcttctttt tcaacaaatg agtctgagaa taatttaaag
     7681 tcttcaagtc agcaaaaagc agatgaaaag gatggtttat acaaagccca attaaacctt
     7741 cttcgattaa tcagccaaaa tgaaacgatc tatcaaaaaa taaaggatta tttatctttt
     7801 tctgagttta ttgaccccgt tttgtctaaa gcagctaaat taatttatga aagttatggt
     7861 gaaggtcgta gtatagaggc tgctaaaatt attaatcatt ttgaaaattt acaggaacaa
     7921 aatcaagtgg ctacgatttt taatgaagaa aaaagtagtg tgaaagaata tgatgttgaa
     7981 aaagcaataa atgatcagat aaagcttatt aaaagagcaa atattgattt aaaaagcaga
     8041 gcgacaactg atatctatga aatacaggct ctgattgagc aaaaaaagca gttggaaaag
     8101 ttgtatatta acattataag tggttaaaat agtagacagg tgagaaagga aggattttgc
     8161 gtgaaaacta gcgaacaaaa caaatttttg acccatttga aggaattagt cgcaatcgca
     8221 aagcaaaaaa agggcgtcct tgaatataaa gaaataatgg atcattttgc cgatatggaa
     8281 ttagaaccag aaaaaattga aaaaatatac gaatatttgg aacgccaaaa tattgatgtt
     8341 attggcatta tcgaagaaga cgaaggcgtt attgaagatg atttagattt aagccttccg
     8401 gaaggtatta gtattgacga ccttgtccgt atgtacttaa aagaaattgg taaagtacct
     8461 cttttaactg cagaagaaga aattgaatta gccataagaa tggaaaatgg tgacgaagaa
     8521 gcaaaaagaa aactggcaga agcaaacctg agattagtag tgagtattgc aaaaagatat
     8581 gttggacgag gtatgctttt tttagactta attcaggaag ggaatttagg tcttattaaa
     8641 gctgtagaaa aatttgacta tcgtaaggga tttaaattta gtacatatgc gacatggtgg
     8701 attcgtcagg cgattacaag ggctattgca gatcaggcac gaacgattcg tatccctgtt
     8761 catatggttg aaaccattaa taagctgata agggtgtcca gacaactcct tcaggaatta
     8821 ggtcgggagc ctacacctga agaaattgca aaagaaatgg acatgcctgt ttcaaaggtg
     8881 cgagaaatta tcaaaattgc acaggaacct gtttctttgg aaacacctat tggggaagaa
     8941 gaagacagcc atttgggaga ctttattcct gatgaagatg tgccagtacc ttctgaagca
     9001 gcagcttata cgcttctgaa agaacagctt atggatgttt tagatacttt aaccgatagg
     9061 gaacaaaaag tattaagact tagatttggc ttagacgatg gaagagcaag gactttagaa
     9121 gaagtaggaa aagtctttaa cgttaccaga gagagaatca gacaaattga agccaaggct
     9181 ttacgaaaac tcagacatcc cagcagaagt aaaaaattaa aagattattt agaatagaag
     9241 ttgactgcca tcaacttctt tttcataatt aaggaatttc tttaaaagct aaacgttgta
     9301 tgcaaaattt ttcaggagga gcctatgata ttatcaaaac gcttatctgc tattgcaaat
     9361 gaagttcctc attatgcaag catagcagat attgggacag atcatgctta tttacctatt
     9421 tatttataca agcataataa aataaagaag gccattgcta cagatatcaa taaagggcct
     9481 ctcgaaaaag caaaaaataa tattatgagc catcaggcgg ataggattat tgaaaccaga
     9541 ttgggaaatg gcctggaagt tttaaaagaa aatgaggttg acacaattat tttagctggc
     9601 atgggcggca tgctgattat tgacatttta aagaaaagac cagaaatcgt tgaaaatatt
     9661 gacaggctga ttattcagcc tcaattggac caaaatgaag tcagaaaata tttacatagt
     9721 attaatc
//
LOCUS       DAOSUD010000076         9721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_3822_length_9721_cov_23.008587, whole genome shotgun sequence.
ACCESSION   DAOSUD010000076 DAOSUD010000000
VERSION     DAOSUD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_9721_cov_23.008587"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLG49_08890"
     CDS             <1..922
                     /locus_tag="PLG49_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17466.1"
                     /translation="FNIIYDAFIIFALWLPVLFMLTFIRNIYFVCTLWLLVFILVYWM
                     LNHFSYDIVLKYSSLAIFIILVLDSLLGSHLQQQSFLSYDPVIGARFYGVGNEYGGII
                     IGSLYLLLYFVSNSPYFVIISLAVQFFLAIILGMPFFGANVGATLSLLTGMISFCIHY
                     SKKKMKSPLFYLTITAFVVFWIFTDLFFVEEKSHLGQMVFQSINGNFRIFTQMIWRKL
                     MMNLQLMRYSLWSKLLAFSLFAVTIKFNLKNDFLKKMAFPFIGAMAGGILMNDSGIVL
                     GAAISIYFVFPYLRSNRRNNQSDHRMECRS"
     gene            919..1338
                     /gene="ruvX"
                     /locus_tag="PLG49_08895"
     CDS             919..1338
                     /gene="ruvX"
                     /locus_tag="PLG49_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOQ17467.1"
                     /translation="MRIMGIDYGEKTIGIAVSDAFGWTAQGIETIKRKEEDNLKESIE
                     RIKALVQEYNVEKIVLGLPKNMNNSLGFRAEKTLKFKEKLEKVLSIPIVLWDERLSTK
                     AAENILLEADLSRKKRKEVIDKMAAVYILQGYLDSLS"
     gene            1355..1630
                     /locus_tag="PLG49_08900"
     CDS             1355..1630
                     /locus_tag="PLG49_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOQ17468.1"
                     /translation="MSGEFKTVFLTDEETGEEVGFEIIDAVEVDGNKYLLVVLMEEDE
                     AQDRAYILKDISQDDKEAVYRLVDDDEEFNKIAVYFMEDNNEYEIEI"
     gene            1728..2168
                     /locus_tag="PLG49_08905"
     CDS             1728..2168
                     /locus_tag="PLG49_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOQ17469.1"
                     /translation="MTIKGMDLKEFLKEKGCKLTTQRRAVLDVLMEHKGKHLSTEEIY
                     EYVKEKCPEIGLATVYRTVQLFEEIKIIDRLNFDDGCSRYELIIDNEEHHHHHLICES
                     CGRVIEVEEDLLEELEERIESKYYFKVKNHKVKFYGLCQKCGAD"
     gene            2184..3554
                     /locus_tag="PLG49_08910"
     CDS             2184..3554
                     /locus_tag="PLG49_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOQ17470.1"
                     /translation="MDKIKIYGKDIEDIELIILCILSSFFLLIYDGKNYSPFACKSQV
                     LYLVAIISIFLLFLKFFVEKSNRLKNTAHIPLFIYAALIIISALFSEYPQVVYKGLPG
                     RLEGVYALLSYITVYFAAYYLIKKEKIYVLIYVLLTFTSIVALIGVLEFYGLNPLDQT
                     YKELRVEHEYKIVIYSTIGNQNFIGSLMCLTLMIGIILFVSSQNKYQKIFFFITSVFL
                     YSGLIVSRTRSAWTGTVLSLVIFLFFLIKKKYIKEVWKKLIFLFMVFFIITFLIAISS
                     EGRIAGKFLAVVRDIKMIEADSKQMDSLGSGRFYIWKNSIPLLTEYFPFGSGPDTFPF
                     VFPSNWEDKMRIFGDPYVIIDKAHNEFLQIGVTTGIPSLLAYLFFIAVIMLKNQIIVF
                     KLDFFSTYDPLILASFIGCIAYLVQSFFNISVVSVAPVYWMILGINQNLVLKYVESSF
                     KETSFS"
     gene            3591..5252
                     /locus_tag="PLG49_08915"
     CDS             3591..5252
                     /locus_tag="PLG49_08915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOQ17471.1"
                     /translation="MATRKSSLKIIPLGGLGEIGKNITAFEYGNSIIVVDCGLAFPED
                     EMLGIDLVIPDVSYLKKNVEKIKGIILTHGHEDHIGALPYVLKEISVPIYGTRLTIGL
                     VENKLKEHNMLKSIERHCVSAGDTIELGPFKVELIRINHSIADSVALAIHTPLGIIIH
                     SGDFKVDYHPINGEPIDLQRFAVLGSRGVLALLSDSTNAERKGYNMSERTVGNVFEDI
                     FSEAVHHRIMVATFASNVDRVQQIINAAYKYKRKVVFIGRSMINVVKTAAELGYLDIP
                     RNTLIELSEINKYNDEELVVIMTGSQGEPMAALSRIASSELRQLELKPGDKIILSASP
                     IPGNEKTISKVINELFRKGADVIYEDAHVSGHAFQEELKLLYSLVKPKYFIPVHGEYR
                     HLKHHAELVMNLGMPKENIFILSNGDVLEFTKNTCKITGTVPSGQILVDGLGVGDVGN
                     IVLRDRKHLSQDGLLVVVITLDRESNKVIAGPDIISRGFVYVRESENLMDEARNVVKN
                     SLTECEDKQITEWAQMKSIIKDTLKEFLWQKTKRSPMILPIIVEV"
     gene            5268..5612
                     /locus_tag="PLG49_08920"
     CDS             5268..5612
                     /locus_tag="PLG49_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOQ17472.1"
                     /translation="MSLDNEINSLIHAILESEQYKNYQRAMMNLSKDEELLKKVNAFR
                     KQRLFLESQAKGNNYPHHELKAVQESYSSLIIFPLAADFLEAEKALSQMLLKIYKNIA
                     EALDFNLDFLNQ"
     gene            5771..6835
                     /gene="mltG"
                     /locus_tag="PLG49_08925"
     CDS             5771..6835
                     /gene="mltG"
                     /locus_tag="PLG49_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ17473.1"
                     /translation="MKKIALKMIKGLFQLGIFCLIVVLCYYVGNKAYNYVYHIANQPS
                     ENERSVKEVIITVEKGATTKEIAKLLEENGLIRSSLLFELKSRYYKYDGKYKEGEFAL
                     STNMSEEEIMEILMTKGAQAEGVRFTIPEGYTVQKIAETLEEQKICTKEEFMKALNEA
                     KYDYKFLDGIPDRSNKFEGYLFPDTYEVRKGADATEIVSKMLNRFDEIVTPEYYKRAA
                     ELGYTMDEIIIIASIIEQEAKLDEERPIIAGVIYNRLNSDINLQMCSTVMYALGKRKE
                     HLLLTDLEIDSPYNTYLYGGLPVGPICNPGAASIRAALYPEEHDYFYFVLKDEETGEH
                     VFTSTGEEHNAAKKKYNQKF"
     gene            6913..7584
                     /locus_tag="PLG49_08930"
     CDS             6913..7584
                     /locus_tag="PLG49_08930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOQ17474.1"
                     /translation="MSDIIYDYINDYIREIIPDSSGLLGQIEREAEKTDLPIISKEVA
                     RMISVLLSIKKPQKILEIGTAVGYSAILMSQYLQEGGQILTIERYDVMYNAAKENIQK
                     AGLENTIYLIEKDAEEVLPNLEDSFDVIFLDAAKGQYNNFLPHCLRLLKEDGLLIADN
                     VLHKGTVAKLRYDVPRRQRTIHKRMRNFLWEIIHNEDLESCILPIGDGVALCYKKTNK
                     AELKG"
     gene            7590..8837
                     /locus_tag="PLG49_08935"
     CDS             7590..8837
                     /locus_tag="PLG49_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOQ17475.1"
                     /translation="MTGKNRKPELLAPAGSLEVLKTAIDYGADAVYIGGYEYGLRAKA
                     KNFTMEEMAEGITYAHKHQVKVYVTANIIAHNQDLIGLDKYFKELAAMGADAVIVADP
                     GVLMVLKETVPDMPVHLSTQANNTNYKGVLFWYKQGVERVVLARELSLKEISEIRSKL
                     PLDLTLEAFVHGAMCISYSGRCLLSNYMTGRDANKGDCAQACRWRYYLVEETRPGEYM
                     PVYENERGTFIYNSKDLCMIDAIPQLIQAGIDSFKIEGRMKTAFYVGSVVKAYREAID
                     DYFEDENLYHSKINYYLEEVTKFSHRDYTKGFYFEKPSGSEQIYSHSSYIRGYSFAGR
                     VMDYDPESQVATIEQRTKFSVGEEIEFMPFSSPSFAQRISQLWDQEGNEIHSAPHPKQ
                     IVKMKVIKPVEKNILIRKKEEDF"
     gene            8915..9589
                     /gene="sigK"
                     /locus_tag="PLG49_08940"
     CDS             8915..9589
                     /gene="sigK"
                     /locus_tag="PLG49_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOQ17476.1"
                     /translation="MFLGILQFIGTFSYFTGNSSFPQPLSPEEEKYYLELYKKGDNEA
                     KNILIERNLRLVAHIVKKYNGLNKELDDLISIGTIGLIKAISSYDLDKGTRLATYAAR
                     CIENEILMSIRSSKKERYEVSLQAPIGIDKEGNEIALIDILYNDSESVLDQVDFKIKL
                     KKLYQKMKTVLKRREKDVLEMRYGLENGSEKTQREIADLLGISRSYVSRIEKKAIKKL
                     SEELAK"
BASE COUNT         3366 a         1240 c         1946 g         3169 t
ORIGIN      
        1 gtttaatata atttatgatg cttttattat ctttgcttta tggctgcctg ttttatttat
       61 gttgactttt atcaggaata tttattttgt ctgtacttta tggcttttag tttttattct
      121 ggtctattgg atgctaaatc atttttcata tgatatcgtt ttaaaataca gttctttggc
      181 tatatttatt atcttagtcc tggattctct tttgggttct catttacagc agcaatcttt
      241 tttatcttat gatcctgtta taggggcacg tttttatggt gtagggaatg agtatggagg
      301 cattattata ggaagtctat atttattact ctattttgtt tctaattctc catattttgt
      361 gataatttct cttgcagttc aattttttct tgctataata ttagggatgc ctttttttgg
      421 tgctaatgtt ggcgcaactc tttcgctttt gacaggaatg atttcatttt gtatccatta
      481 cagcaaaaag aaaatgaaat ctcctttatt ctatcttaca ataacagcct tcgttgtttt
      541 ttggattttt acagatttgt tctttgtgga agaaaaatct catttgggac agatggtttt
      601 tcaaagtata aatgggaact ttagaatctt tacccaaatg atttggagaa aattaatgat
      661 gaatttgcaa ctaatgaggt attccttatg gtcaaaatta ttagcatttt ccctttttgc
      721 agtaacgatt aagtttaatt taaagaatga ttttttaaaa aaaatggcgt ttccttttat
      781 tggagctatg gcaggcggaa tattgatgaa tgactcagga attgtgttag gggcagctat
      841 ttccatatat tttgttttcc cgtatttgag aagcaataga agaaataatc aatctgatca
      901 tagaatggag tgtagaagtt gagaatcatg ggaatagact atggggaaaa gactataggg
      961 attgcagtga gtgatgcttt tggctggact gcccagggga ttgaaactat taaaagaaaa
     1021 gaagaagata atttaaaaga aagtattgaa cgcatcaagg ctttggttca ggaatacaat
     1081 gtggaaaaaa tcgtattagg gctccccaaa aatatgaata acagcttagg ttttagggca
     1141 gaaaaaactt taaagtttaa agaaaagctg gaaaaggttc tttccattcc cattgtttta
     1201 tgggatgaaa gactaagtac aaaagctgca gaaaatattt tattggaagc agatttaagc
     1261 aggaaaaaaa gaaaagaagt cattgataaa atggcagcag tatatatttt gcaaggttat
     1321 ttggatagtt taagctaaaa ggaggaaaat gagtatgtct ggagaattta aaacggtatt
     1381 tttaaccgac gaagaaactg gtgaagaagt tggttttgaa atcatcgatg cagtcgaagt
     1441 agatggcaat aaatatttat tggtcgttct catggaagag gacgaagcac aagatcgggc
     1501 ttatatttta aaagatatta gtcaggatga taaagaagca gtttacagat tagtagatga
     1561 cgatgaagaa tttaataaaa ttgctgtgta ttttatggaa gataataatg aatatgaaat
     1621 agagatttaa tgaaattgga tatcaattga caaaaagcgc tttctattat atactactgg
     1681 taattattat gaaacggcag ataggattaa aattatggag gtattcaatg accataaaag
     1741 gaatggattt aaaagagttt ttaaaagaaa aaggctgtaa gttaacaaca caaagaaggg
     1801 ctgttttaga tgtattaatg gagcacaaag ggaaacactt gagtacagag gagatatatg
     1861 aatatgttaa agaaaaatgt cctgaaatag gtcttgccac ggtgtacaga acagttcagt
     1921 tgtttgagga aataaaaatc atagaccgct taaattttga tgatggttgt agtcgttatg
     1981 aattaattat tgataatgag gaacatcatc atcatcattt aatctgtgaa tcctgcggaa
     2041 gagttattga ggttgaagaa gatttactgg aagaattgga agaaagaata gaaagtaagt
     2101 attattttaa agttaaaaat cataaggtta agttttatgg cttgtgtcaa aaatgtggtg
     2161 cagattaatt tgggggggtt gtgttggata aaatcaaaat ttatggaaaa gatatagaag
     2221 atatagaatt gattattcta tgtattttat ccagcttttt tttgcttata tatgatggta
     2281 aaaattattc cccatttgcc tgtaagtctc aagtattata tttagtagca ataatttcaa
     2341 tttttctttt attccttaag ttttttgtag aaaagagtaa ccgactaaaa aatacagccc
     2401 atattccttt attcatttat gcagctttaa ttataatatc agccttgttt tctgaatatc
     2461 cccaagttgt atataaaggt cttcccggaa gactggaagg tgtgtatgcc ctcttatcat
     2521 atattacggt gtattttgct gcttattatt taattaagaa agaaaagata tatgtactta
     2581 tttatgtttt attgactttt acatctattg tagccctgat aggggtactg gagttttatg
     2641 gacttaatcc cctggatcaa acctataaag agttaagagt ggagcatgaa tataaaattg
     2701 taatttattc cactataggt aatcaaaatt ttattggttc tcttatgtgt ttgactctaa
     2761 tgattggtat tattttgttt gtttccagtc aaaataaata tcaaaagatt tttttcttta
     2821 taacttcagt ttttctttat tcaggactga ttgtaagccg taccaggagt gcctggacag
     2881 gtaccgtgct tagccttgtt atttttttat ttttcttgat taaaaaaaaa tatataaaag
     2941 aagtttggaa aaaattgatt tttttgttta tggttttttt tataattacc tttttgatag
     3001 caatcagttc agaaggcaga attgctggta aatttttggc ggtagtaaga gatataaaaa
     3061 tgattgaagc tgattctaag caaatggatt ctttaggaag tggtcgtttt tatatctgga
     3121 aaaattcaat tccactttta acagaatatt ttccctttgg ttcaggacca gacacatttc
     3181 cctttgtttt tccttctaac tgggaagaca agatgaggat atttggagat ccttatgtca
     3241 tcatcgataa agcacacaat gaatttttgc aaattggagt tacaacaggg attcccagtc
     3301 ttttagcata tttatttttt attgctgtta ttatgttaaa gaatcagata atagttttta
     3361 aattagattt ctttagtaca tatgatcctt taatattagc ctcatttata ggatgtattg
     3421 cttatttagt gcaaagtttt tttaacattt cagttgtgtc tgtagcacct gtttattgga
     3481 tgatacttgg tattaatcaa aatttagtat tgaaatatgt ggaatcttct tttaaggaga
     3541 catcatttag ttagctttaa aattatttta tgtttactgg aggtgtagtt ttggcaacaa
     3601 gaaaatcaag tttaaaaata atccccctgg ggggtttagg tgaaataggt aaaaatatta
     3661 ctgcttttga atatggcaat agcattattg ttgtagattg cggcttagct tttccggaag
     3721 atgagatgct aggaattgat ctggtgatac cagatgtaag ttatctgaaa aagaatgtag
     3781 aaaaaattaa aggaattatc ttgacccatg gtcatgagga tcatattgga gctttacctt
     3841 atgtattaaa ggaaatcagt gttcctattt atggaactcg tttaacgatt ggtttggtag
     3901 aaaataaact taaagaacac aacatgttaa aatccatcga aaggcattgt gtatctgccg
     3961 gcgatacgat tgaactggga ccatttaaag tggaattaat aagaattaat catagtattg
     4021 cagattctgt agctttagca attcatacgc ctctgggtat cataattcat tcaggggatt
     4081 ttaaagttga ttatcaccct attaatggtg aacccattga tcttcagcgt tttgctgttt
     4141 taggaagccg gggcgtgctg gcccttttat cagacagtac caatgctgaa agaaaaggct
     4201 ataatatgtc tgagagaacc gtaggaaatg tttttgaaga tattttttca gaggcagttc
     4261 atcaccgtat tatggtagcg acttttgctt ccaatgtgga tagggttcag caaattatta
     4321 atgcagcgta taaatataaa agaaaagtgg tttttattgg ccgaagtatg atcaatgttg
     4381 ttaagacagc tgcagaatta ggctatttgg atatacccag aaacactctt atagaattaa
     4441 gtgaaattaa taagtataac gatgaagaat tggttgtaat tatgactggc agtcaggggg
     4501 aacctatggc agctttatcc agaattgctt catcggaact cagacaatta gaattaaagc
     4561 caggtgataa aattattctt agtgcttccc caattccagg aaatgaaaaa accatttcaa
     4621 aagttatcaa tgagttattc agaaaaggtg cagatgttat atacgaagat gcccatgttt
     4681 cgggtcatgc ttttcaggaa gaattaaaac ttttatatag cttagtaaaa ccgaaatatt
     4741 ttataccagt tcatggcgaa tatcgccatt taaaacatca tgctgaatta gtaatgaatt
     4801 taggaatgcc taaggaaaat atttttattc tgtccaatgg ggatgtgctt gaatttacaa
     4861 aaaatacttg taaaataact ggtaccgttc cctctggtca gatcctggtc gatggcttag
     4921 gtgtaggaga tgtgggaaat atcgtactta gagacagaaa acatctttcg caggatggtc
     4981 tgttggttgt agtcattacc ttagacaggg aaagcaataa ggttattgct ggtcccgaca
     5041 ttatttccag aggttttgtg tatgtaagag aatctgaaaa cctcatggat gaagccagaa
     5101 atgtagtaaa aaattcctta acggaatgtg aagataagca aattacagaa tgggcgcaaa
     5161 tgaaaagtat tattaaagat accctaaaag aatttttatg gcagaagacc aaaagaagtc
     5221 ctatgatttt accgattatc gttgaagtat aaaaaagggg gacggcaatg agtctggata
     5281 atgaaataaa ttcccttatt catgccatac tggaaagcga gcaatacaaa aattatcaaa
     5341 gagcaatgat gaatttgtca aaagatgagg aattgctgaa gaaagttaat gcttttcgga
     5401 aacagcgctt atttttggaa tcccaggcaa aaggaaataa ttatccgcat catgaattaa
     5461 aagctgtgca ggaaagctat tcttcattaa taatttttcc tcttgcagct gattttttag
     5521 aggcggaaaa agcgttaagt cagatgcttt taaaaattta taaaaatatt gcagaagcct
     5581 tagactttaa tttagatttt ttaaatcaat aatgtttatc tgtcaggagc cagttgtaaa
     5641 aactggctct tatataaaaa atattaactg accttgctcc ctattcaaat aatcagaata
     5701 agtttttggt tgaatcttgt attaaatctc tgtataatat gaatagattt taacagaata
     5761 gggtgaaaaa atgaaaaaga ttgcattgaa gatgataaaa ggcttgtttc agttaggaat
     5821 attttgttta atcgtcgtat tatgttatta tgttggaaat aaagcttata attacgtata
     5881 tcatattgct aaccaacctt cagaaaatga aagaagtgtt aaagaagtca taataacggt
     5941 cgaaaaagga gctactacga aagaaattgc taaactttta gaagaaaatg gcctaatccg
     6001 tagcagctta ttatttgagt taaagagcag atattataaa tatgatggaa aatataaaga
     6061 aggcgaattt gctttatcca cgaacatgtc agaagaagaa attatggaaa ttcttatgac
     6121 aaaaggtgcc caggcagaag gtgtcagatt tactattcca gaagggtata cggttcaaaa
     6181 aattgctgaa actttagaag aacaaaagat ttgtacaaaa gaagaattta tgaaagcatt
     6241 aaatgaagcc aaatatgact ataagttttt agatgggata cctgacagaa gtaataaatt
     6301 tgaaggctat ctttttcctg atacctatga agtaagaaaa ggtgccgatg cgacggaaat
     6361 tgtaagtaaa atgctcaacc gtttcgatga aatcgttacg ccggaatatt ataagagggc
     6421 tgcagagttg ggttatacta tggatgagat tattattatt gcttccatca ttgaacagga
     6481 agctaaatta gatgaagaaa gacctattat tgcaggtgtt atttataatc gtttaaattc
     6541 cgatatcaat ctacagatgt gttcaaccgt aatgtatgct ttaggtaaaa gaaaagaaca
     6601 ccttttatta acggatttgg agattgattc tccttataat acttatttgt atggtggact
     6661 gcctgtgggt ccgatatgca atccaggagc agcttccata agagcagccc tgtatccgga
     6721 agaacatgac tatttttatt tcgttttaaa agatgaagaa acaggtgaac atgtctttac
     6781 ttctactgga gaagaacata atgcagcaaa gaaaaaatat aatcagaaat tttagggaaa
     6841 gatataaaga cgaaagaaat ttctttcgtc tttataattt tgattgtgac cagttttata
     6901 caggaggtta atatgagtga tataatttat gattacatta atgattatat aagagaaatt
     6961 atacctgaca gttcaggcct tttaggtcag attgaaagag aggctgaaaa aactgattta
     7021 cccattatat ctaaagaagt tgcccgcatg atatcagttt tattatctat aaaaaaaccg
     7081 caaaaaatct tagaaatagg gacagcagtg ggttactcag cgattttaat gagtcagtat
     7141 cttcaggagg gtggtcagat tcttactata gaaagatatg atgtgatgta taatgcagca
     7201 aaagaaaata ttcaaaaagc aggacttgaa aataccatat acttgataga aaaagatgct
     7261 gaagaagttt tgccaaattt agaggattct tttgatgtga tttttttaga tgctgcaaag
     7321 gggcaataca ataattttct tccacattgt ctgagacttt taaaagaaga tggtttactg
     7381 attgccgaca atgttcttca taagggaaca gtggccaagc ttagatatga tgtaccaagg
     7441 aggcagagga ccattcataa aagaatgcgg aattttttat gggaaatcat tcataatgaa
     7501 gatttagaaa gctgcatact tcccatagga gatggagtgg ccctgtgtta caaaaagacc
     7561 aataaagctg aattgaaagg atgaaaaagg tgacgggaaa aaatagaaag cctgaattgc
     7621 tggctccagc aggcagttta gaagtcttaa agactgctat tgattacggg gcagatgctg
     7681 tttatatagg aggttatgaa tatggcttaa gggcgaaagc gaaaaatttt actatggaag
     7741 aaatggctga aggaataacc tatgcccata aacatcaggt aaaagtgtat gtaacagcaa
     7801 atattattgc ccataatcaa gacttaattg gcctagataa gtattttaaa gaacttgccg
     7861 ctatgggtgc tgatgctgtg atagtagcag acccaggagt acttatggtt ttaaaagaaa
     7921 cagttccgga tatgcctgtt catttaagca ctcaggcaaa taacacaaat tataaaggcg
     7981 tattgttttg gtataaacaa ggtgttgaaa gagtcgttct tgccagggaa ttgtctttga
     8041 aagaaatcag tgaaataaga agtaagctgc ccttagattt aactctggaa gcttttgttc
     8101 acggggcaat gtgtatttcc tattctggcc gttgcttgtt aagcaattat atgacaggaa
     8161 gggatgccaa taagggggac tgtgcgcagg catgccgctg gcgctattat ttggtcgaag
     8221 aaaccagacc aggggaatat atgcctgttt atgaaaatga aagaggtact tttatttaca
     8281 attcaaaaga tttatgtatg attgatgcta tccctcaatt aattcaggca ggtattgaca
     8341 gttttaaaat tgaaggccgg atgaaaactg ctttttatgt tggttcagtg gtaaaggctt
     8401 acagggaagc cattgatgac tattttgagg atgaaaactt atatcattct aaaataaact
     8461 attatttaga agaagtaaca aaattcagtc acagggacta tacaaagggt ttttattttg
     8521 aaaagccttc aggcagcgaa caaatttaca gccatagcag ttatatcaga ggatacagtt
     8581 ttgctggcag agtcatggat tatgatcccg aaagtcaagt ggctacgatt gagcaaagaa
     8641 ccaaattttc tgttggggaa gaaattgaat tcatgccttt ttcgagtcca tcctttgctc
     8701 aacgaattag ccagctttgg gatcaagaag gtaatgaaat ccattcagca ccgcacccaa
     8761 agcaaattgt aaaaatgaaa gtaataaagc ctgttgaaaa gaatatatta ataaggaaga
     8821 aagaagaaga tttttagtaa ataaaatgag acagacacat ctttgccgga agcttcatag
     8881 aataaaaaag aagcttttag cggaggtgtg ttttttgttt ttagggatac tgcaatttat
     8941 tggtactttc tcgtatttta caggaaattc ttcttttcct cagcctctta gtccagaaga
     9001 agaaaagtat tatttagaat tgtacaaaaa aggtgataat gaagccaaaa atattctgat
     9061 agaaagaaac ttacgcttag ttgctcatat tgtgaaaaag tacaatggtt tgaataaaga
     9121 attggatgat ttaatttcta taggaaccat aggacttata aaagccatta gctcttatga
     9181 cttggataag ggtacaaggc ttgccactta tgcggcaaga tgcattgaaa atgaaatttt
     9241 aatgagcatc cgttcttcaa agaaagaacg ctatgaagta tccctgcaag cacctattgg
     9301 aatagataaa gaaggaaatg aaattgcatt aatcgatatt ttatacaatg attcggaatc
     9361 tgtattagac caggtggatt ttaaaataaa actaaaaaaa ttatatcaaa aaatgaaaac
     9421 agttttaaaa agaagagaaa aagatgtctt ggaaatgcgt tatggtttgg aaaatggcag
     9481 tgaaaaaacc caaagggaaa tagcagattt attaggaata tctcgttctt atgtctccag
     9541 aatagagaaa aaagccatta aaaaattaag tgaagaattg gcaaaataaa ggcacttatt
     9601 tttagtgctt tatttttata tttaaaatag aaataaatat aggatttata caaataatta
     9661 catatgttat aataaacaaa aaagtaagta ttaatataaa ttatggtaga ttaaaaatat
     9721 t
//
LOCUS       DAOSUD010000077         9180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4023_length_9180_cov_26.068493, whole genome shotgun sequence.
ACCESSION   DAOSUD010000077 DAOSUD010000000
VERSION     DAOSUD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_9180_cov_26.068493"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..2206
                     /locus_tag="PLG49_08945"
     CDS             359..2206
                     /locus_tag="PLG49_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ17477.1"
                     /translation="MQALILAAGMGKRLKDLTRDSAKCMVKVNGKTLIERMLLQLDAL
                     KLKEIIIVVGYQGEKLISYINSLNIQTPVSFIKNDAYSKTNNIYSLYLAKDYLVKDDT
                     LLLESDLIFSDEILKRIINNPYPDLALVAKYESWMDGTVVTLDSQNNICSFVEKCEMD
                     YNKLDQYYKTVNIYKFSKEFFKSCYFPFLKAYIDSQCCNEYYEQVLKVLSFIKKSPIK
                     AEVLEDEPWYEIDDLQDLDIAESIFSEKSFEKMKKRYGGYWRYPKLIDFCYLVNPFYP
                     PGKMIEEMKANFEQLICNYPSGMEVNSLIAGKYYGIKKEHICPGNGAAELISSLMKTV
                     KGHVGVIFPTFEEYPNRLNQNYIVLYYPRNKNFAYTAQDLMDYYRKFNIEALILINPD
                     NPSGNFIEKTELLRLAAWAEQKGIRLIVDESFVDFTDAGEAQSLLSEEILQKYKNLAV
                     VKSISKSFGVPGLRLGIIASGDEALIDFIKRDLPIWNINSLAEFYLQISEKYKKDYQL
                     ALEKFKLIRKEFTEQLKKLPHLRVIPGQANFVMCELMGPCSAEELSESLLNEYNILIK
                     DLSGKKGIDGQYIRLSIKTREENDLLLKSLHRILNQVSRSKTVNLHFTA"
     gene            2219..2860
                     /locus_tag="PLG49_08950"
     CDS             2219..2860
                     /locus_tag="PLG49_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOQ17478.1"
                     /translation="MSKELIQAQRDYTMLYFVKDPANLCSLTGLLASFFAIYFAVTKQ
                     HHYALACALWAVVFDWLDGRIARRLKNRSDYHRAIGVQMDSLIDIVSFGVFPAFFLLS
                     YGNFGPVFMPGASMIVAAAAVRLSYFNVFGMIDKNTYKGLALDNNVIILALIFILEKF
                     LPQQSFSIFLYLALILLAVFNLAPVPTPKLGDKWLPVLFVYTLVMSVYYIIFY"
     gene            complement(2967..3728)
                     /locus_tag="PLG49_08955"
     CDS             complement(2967..3728)
                     /locus_tag="PLG49_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ17479.1"
                     /translation="MLLLSILLVFIASFAITARALVPIRKSWERQVQFTADASHELRT
                     PIAVIQANLEVVMDEPEQTVAKKQKWLNHIKAEADRMQKLIEDLLILSRADEGQENLE
                     KSLFNLSETLINTLESLIPYAQSKGIKLKTDIEADISFFGDKEQIKQLLVILVDNAIK
                     HTPSGGEVLVKSQIQNRNLVIKVTDTGEGIAKEHLTKIFDRFYRINKARERESGSSGL
                     GLSIAKWIVEEHRGSIHVESKLGAGSTFTVVLPPK"
     gene            3955..5730
                     /locus_tag="PLG49_08960"
     CDS             3955..5730
                     /locus_tag="PLG49_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ17480.1"
                     /translation="MNCIDYFVAGAYYDRDNILQITTEYIHLLEENGYEVLSANISKT
                     PGVSMHLEEYEGALFIFQVFSLLAFIFGCLIMASLFSTMLAGQVKQIGILKSMGARTG
                     KIKFAYMGAAGILIAFNLAVSLPLSVVIGRGLSAFFLRIGNMYLTRFDLSPVVYILVT
                     AALVTVPLLLSYLPVRRGLAIGVKDAINDYGIQVRIQELKIPHRCLNRLSRPVLLSVR
                     RALEHRRRFIMNLMMLTLGGLLFVGIMGNIVSINKALSDNMEARHFDYQFTSSHYKDM
                     DKIKKALDANPEMVAYYEVWGEATGKLIYPDGHTGNLYALTAMDYNSKLFTPEMIEGR
                     WLQAGDSNAIVVSFEFLNNEPYALGDKVLFQFGSITQEMTIVGIIKEIGSQSIYMEIK
                     GFETLIPPKAQRISVPVKTAIQKRNMRRVYKDLEEYLSISEISIVQSESKMERYKILK
                     AHFTTTLTSFLTVAILVVFVAGFGLASAMNLQVSERTREIGIMKSMGASNRQIKKIVR
                     TESTFVCLCSWLLSLLLAIPVLFLSIYYIGVYVLEIPLTSSWLALILSMLAWFVFTWF
                     IGRFASRSAGKRAARMTVRRAIIQE"
     gene            complement(5998..6981)
                     /gene="bioB"
                     /locus_tag="PLG49_08965"
     CDS             complement(5998..6981)
                     /gene="bioB"
                     /locus_tag="PLG49_08965"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOQ17481.1"
                     /translation="MTEKMTIKELLSKVIEGKDISLEEANLLIRPDLTDLNELIQASD
                     IITKHYFNNTVEMCAIYPAKMGKCSGDCAFCAQSVHHNSKITPISVQELKLDDIISKA
                     KDLEKFGTKHFSLVTSSETLNDEEFNLIIHIFYRLNKETTLKLCASLGSLTLEKAKKL
                     KNAGVSRYHHNIETSRSFFQKICSTHSYDDKIETIKTAQQAGLEICCGGIISMGETAE
                     QRVEMAFALKNLNVDCIPINILNPIPGTPLENQKLLSAEEILRTIAVFRFILKDKTLR
                     FAGGREKALAEHEYLGYRAGINALLIGDYLTTPGKSIENEKENLKMAGLII"
     gene            complement(6996..7625)
                     /locus_tag="PLG49_08970"
     CDS             complement(6996..7625)
                     /locus_tag="PLG49_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOQ17482.1"
                     /translation="MKNKKAKSVLSAKELSVIALFTAITAVFAQIAIPLPFTPVPISF
                     GLVAVYMSGILLKPKHAVYSQLCYLTLGGLGIPVFGGFRGGLGALFGPTGGYLLVYPI
                     MAWLVSMVLNSQKSRQNESSNSKGFLLFKSAVAICTAHIILYLGGTTWLSITTGNSFY
                     ATLAIAVFPFIPMDILKIIFCIFAVVPIRSRLISTNFLIIDNTKADFSE"
     gene            8182..8820
                     /locus_tag="PLG49_08975"
     CDS             8182..8820
                     /locus_tag="PLG49_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17483.1"
                     /translation="MNVYYEKEKDAATDTFNYADTATEFIAKKLGFTEKENINIYIYD
                     SQATMQRKKYGFIGPLLGLDWYIGDNIGTNVILTSPANPGAMHNYDSVKYAVLHEIVH
                     AYISVLNPDIDLWLTEGVALYLANGEEFYKEYLETMSIPSYSDICTRNPVRFSNCGGY
                     TFATTYVEYLDVTYGWDSVLELIKTEDYMLCFGKSQEDIYQEWVEYIKNYYQ"
     gene            8873..>9180
                     /locus_tag="PLG49_08980"
     CDS             8873..>9180
                     /locus_tag="PLG49_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17484.1"
                     /translation="MITYGNKISEKEYNDLRKAVGWNTLSKEQAERGLMNSAFLTVAY
                     KDGKAVGMARVVGDGGYIVIIADVMVLPEFQGQGIGRQLMNNVMDYLKNSMQKNDFLM
                     V"
BASE COUNT         2867 a         1423 c         1872 g         3018 t
ORIGIN      
        1 aaaaggctgt tattcaactt ttgttgagta acagcctttt tataaaaaag cctattgaca
       61 aaatttaaaa aaaatgatat ataaaatttg agtttagcac tcgcgattta tgagtgctaa
      121 cagcaataaa gtcatagctg tgtgaatgtg cagcgctaaa ttaaaggaga agtaaaagcc
      181 atttttattt gttatttttt aatttactat agataactaa aagataaaaa tcttatcccc
      241 cctaaaattt aaaccaattt ttattttgac agcgctgtat atttatatgg cgctatttct
      301 gttgttaaaa ataagcatgc atgctttttt gacaaaaata taaaaagagg tgaaagatat
      361 gcaggcttta atattagcag caggaatggg aaaaaggtta aaagacttaa ccagagattc
      421 agcaaaatgc atggttaagg ttaatggaaa aactttaatt gaaagaatgc ttttacaact
      481 tgatgcgtta aagcttaaag aaattatcat tgtggtgggg tatcaggggg aaaaattaat
      541 atcatatatt aattccctaa acattcaaac gcctgtaagc tttatcaaaa atgatgcata
      601 ttctaagaca aacaacattt attcactgta tttggcaaag gattatttag ttaaagatga
      661 taccttgctg ttagaatctg atttgatatt tagcgatgag attttaaaaa gaatcattaa
      721 caatccctat cctgacctgg ctttagtggc taaatatgaa agctggatgg acggaacggt
      781 tgttacttta gacagtcaaa ataatatctg cagttttgta gaaaaatgtg aaatggatta
      841 taacaagctt gaccaatatt ataaaactgt aaatatttat aaatttagca aagaattttt
      901 taaatcctgt tattttcctt tccttaaagc ctatatcgat tcacaatgct gtaatgaata
      961 ttatgagcaa gtccttaaag tattaagctt tatcaaaaaa tctcccatta aagcagaagt
     1021 tttagaagat gaaccatggt atgaaattga cgatttacaa gatttggata tagcagaatc
     1081 cattttttct gaaaagagtt ttgaaaaaat gaaaaagagg tatggcggat actggagata
     1141 tcctaaactg attgattttt gctatctggt gaatcctttt tatccgcctg gaaaaatgat
     1201 tgaagaaatg aaagcaaatt ttgaacagtt gatttgcaat tatccatcag gtatggaagt
     1261 aaacagtctg attgccggga aatattacgg aatcaaaaaa gaacatattt gtccaggcaa
     1321 tggggcggcg gagctcattt cttctctgat gaaaacggtt aagggtcatg ttggtgtgat
     1381 tttccccact tttgaagaat atccaaatcg cttaaatcaa aattatattg tgctgtatta
     1441 tccaaggaat aagaattttg catatacagc acaagactta atggattact atagaaaatt
     1501 taatatagaa gccctgattt taattaatcc tgataatccc tccggtaatt ttattgaaaa
     1561 aactgagtta ttaagattag cggcctgggc tgaacaaaaa gggataaggc tgattgtaga
     1621 tgaatctttt gtagatttta cagatgctgg agaggctcaa agcttacttt cagaggagat
     1681 tcttcaaaaa tacaaaaatt tagctgttgt caagagtatt tcaaagtcct ttggtgtacc
     1741 aggcttgcgt cttggtatta ttgccagtgg ggatgaagcg ttaattgatt ttatcaagag
     1801 agatctgcct atatggaata tcaattcatt ggccgaattt tatcttcaga tttctgaaaa
     1861 atataaaaag gattatcagc tggccctgga aaaattcaaa ttgatacgaa aagaatttac
     1921 agagcaatta aaaaagctgc cccatttaag agttataccc ggccaggcca attttgtaat
     1981 gtgtgaatta atggggcctt gttcagctga agaattgtct gaaagcttgc taaatgaata
     2041 caatatattg ataaaagatt tatctggaaa gaaaggaatt gatggacaat acatcaggct
     2101 gtcaattaaa acgagggaag aaaatgattt actgctgaaa tcccttcatc gtatacttaa
     2161 ccaggtaagc aggtctaaaa ccgttaacct tcactttact gcataggagg ggcttgatat
     2221 gagcaaagaa ttaatccaag ctcaaagaga ttatacgatg ctttattttg taaaagatcc
     2281 tgccaatctt tgttcgctta caggtctgtt ggcttctttt tttgcaatat attttgcagt
     2341 gacaaagcaa catcattatg ctttggcatg cgccctttgg gcagtcgttt ttgattggct
     2401 ggacggacga atagcccgtc gtcttaaaaa ccgaagcgat tatcaccgtg cgataggcgt
     2461 tcagatggat tctcttattg atattgtcag ttttggcgtt tttccagctt tctttttatt
     2521 aagttatgga aatttcggcc cggtatttat gccaggagcg tctatgatag tagcggcagc
     2581 agcagtccgt ttaagttatt ttaatgtgtt tggcatgatt gataaaaata catataaagg
     2641 cttagcctta gataataatg ttattatatt ggctttaatt ttcatcctgg aaaagtttct
     2701 gcctcagcaa tcgttttcga tattcctata tttagcttta atactccttg cagtttttaa
     2761 tcttgcccct gttcccaccc ctaagctggg agataaatgg cttcctgtac tctttgtata
     2821 tacgcttgtt atgtcagttt attatatcat tttttattaa tatcactgtt ttttgtaaaa
     2881 tcatttgatt ttcagataaa gacagatgat ttctcctgcg tacctgttaa aaagtaaagc
     2941 actggcaaag ccagtgcttt acttttttat tttggtggga gaacgactgt aaaggtgctg
     3001 ccagcaccta atttgctttc aacatggatg gaaccccggt gttcttccac aatccatttg
     3061 gcaatggaaa gacccaggcc tgaactgcca ctttcccttt cccgtgcttt gttaatccgg
     3121 tagaaacggt caaagatttt agtcagatgt tcttttgcta tcccttctcc tgtgtcagta
     3181 actttaataa caagattgcg gttttgtatc tggcttttga ccaggacttc tccacctgaa
     3241 ggggtatgtt tgatggcatt atcgacgagt attaccagca gttgttttat ctgttctttg
     3301 tctccaaaga atgatatatc cgcttcaatg tctgttttta attttatccc cttgctttgt
     3361 gcataaggta tgagagactc taaagtattt atcaatgttt ctgaaagatt aaagagagat
     3421 ttttctaaat tttcctgtcc ttcatcagcc cgggagagga ttaataaatc ctctattaat
     3481 ttctgcatcc ggtctgcttc agctttaata tgattaagcc atttttgctt tttggcaacc
     3541 gtttgttctg gctcatccat aacgacttca agattggcct gaatcaccgc tatgggtgta
     3601 cgtaattcat gggaagcgtc agcggtaaat tggacttgcc gttcccagga ttttcgtatg
     3661 ggaaccagtg cccgcgctgt gatggcgaag ctggctataa acacgagcaa gatacttaga
     3721 agcagcatag ggctaatacg ggaaagatat gatgtaaggg cttgtccgta gcttatggat
     3781 tcttgcaata caatttgtgt gttggctccg gccttatgta taaaacgcag ataaatatag
     3841 ccttgcccgt tataagttat ttgtccactg gctgaatcct tttccaggat aagatttgcc
     3901 agttcagtat atgcttatgt atcagaagag accctgaaat ctatggggct tgaaatgaat
     3961 tgcattgatt attttgttgc aggagcgtat tatgacaggg acaatatttt gcaaattaca
     4021 acagaataca ttcacctgtt ggaggaaaat ggttatgaag tattgtctgc taatatatca
     4081 aaaacgcctg gtgtgagtat gcatcttgag gagtatgaag gtgccctgtt tattttccag
     4141 gttttctcac ttttggcttt tatatttggc tgcttaatta tggccagtct tttttccaca
     4201 atgcttgctg gtcaggtaaa acaaattggc atattaaaat ccatgggtgc acgaactggc
     4261 aagataaagt ttgcctatat gggagcagcc ggaatattaa ttgcttttaa tctggctgtg
     4321 tcattacctt tatccgtagt tatcggcaga ggtttgtctg ctttcttttt gcggatagga
     4381 aatatgtatc tgacaagatt tgatctgtct cctgttgtat atatccttgt aactgctgca
     4441 cttgttacag tgcctttact gctttcttat ttgcctgtac gccggggact tgcgatagga
     4501 gttaaagacg ctattaatga ttatggaata caggtcagaa ttcaagaact taagatacct
     4561 cacagatgcc ttaaccgttt gtcaagacca gtgttactgt ctgtacgcag ggctctcgaa
     4621 caccgccgcc gttttattat gaatttgatg atgttaacac ttgggggtct cttgtttgtg
     4681 ggtataatgg ggaatatcgt ttcaattaat aaggctttat cagataatat ggaagcacgc
     4741 cattttgact accagtttac gagcagtcat tataaagata tggataaaat taaaaaagca
     4801 ctggatgcta atcctgaaat ggtggcttac tatgaagtgt ggggtgaggc aacaggaaaa
     4861 ctcatttatc cggatggaca tacagggaat ttgtatgccc tgacagccat ggattataat
     4921 agtaagttat ttacacctga aatgatagaa ggccgttggt tgcaagcagg tgatagcaat
     4981 gccattgtag taagttttga atttttaaac aatgagccat atgctttggg agataaggtc
     5041 ttattccagt ttggcagtat tacacaggaa atgaccatag taggcattat caaagaaatt
     5101 ggaagccaga gcatatatat ggaaataaag ggttttgaga cccttattcc gccaaaggct
     5161 cagagaatta gtgttccagt caaaacagca atccaaaaga gaaatatgcg aagagtttat
     5221 aaagatttgg aagaatatct ttctataagc gaaatatcga ttgttcaatc agaaagtaag
     5281 atggagcgct acaagattct taaggctcat tttactacaa cattgacgtc ctttttaacc
     5341 gtagcgattt tagttgtgtt tgttgctggc tttggccttg cttctgctat gaatttgcaa
     5401 gtgtcagagc gtacccggga aatcggtatt atgaaatcga tgggtgccag caacaggcaa
     5461 atcaaaaaaa tagtccgaac agaaagtact tttgtatgtc tgtgtagttg gcttcttagt
     5521 cttttgctgg ctatacctgt tttattctta agcatttact atataggcgt ttatgttctg
     5581 gagattccac tgacatcttc atggcttgca cttattcttt ctatgctggc atggtttgtc
     5641 tttacatggt ttattggccg ttttgcaagc cgttctgcag gaaagcgggc cgcaaggatg
     5701 acagtacgca gggcaattat tcaggaataa aaaattaccg gggcttttta ttgaccccgg
     5761 tttttaaggt ataaaaaatt cttaaaattt attctagaat atagtacgct tttgctactt
     5821 taaactttat taggggaatt aaggtgataa aggctgcata aaagcttagg gggaagaaaa
     5881 gagggaaatt ggatttatta atcactaagg tcgcttttta tcatttaccc gatttttgga
     5941 agcagattca aaaatgaaag taaatggatg cataaaatat gaagatggcc ttaaagatta
     6001 tatgattaag ccagccatct tcaagttttc tttttcattt tcaatacttt ttccaggtgt
     6061 tgtcaggtaa tcccctatga gcaaagcatt aattcctgca cgatagccca aatattcatg
     6121 ttcagccaga gctttttctc tacctccggc aaatcgtaaa gtcttgtctt ttaatataaa
     6181 gcgaaagact gctatggttc ttaggatttc ttctgcggaa agaagctttt gattttctaa
     6241 tggggtgcca gggataggat tgagtatgtt aataggaatg caatcaacat ttaaattttt
     6301 taaagcgaaa gccatttcca cacgttgctc tgcggtttct cccatagaaa taataccgcc
     6361 acagcagatt tctaaacctg cctgttgggc agttttgata gtttctattt tgtcgtcata
     6421 gctatgggtt gagcaaattt tttggaaaaa actacggctt gtttcaatgt tatggtggta
     6481 gcgggatacc ccggcatttt tcaatttttt agctttttct aaagttaagc tgcccaaaga
     6541 tgcacagagt tttaatgtcg tttccttatt tagcctataa aatatatgga tgataaggtt
     6601 gaattcttca tcatttaatg tttcactact tgtgacgagg ctaaaatgct ttgtcccgaa
     6661 cttttctaag tctttagctt ttgagataat atcatctagt tttaattctt gaactgaaat
     6721 aggcgtaatt ttactgttgt ggtggacaga ttgagcacaa aaagcacagt ctcccgagca
     6781 ttttcccatt ttagccgggt atatggcaca catttcaact gtattattaa aataatgttt
     6841 ggtaatgatg tcagatgcct gaattagttc atttaagtcc gttaaatcag gtcttatgag
     6901 cagattcgct tcttccaaag agatatcttt tccttctata actttgctta ataattcttt
     6961 gatagtcatt ttttctgtca tgattccggc tccttttatt cagaaaagtc tgcttttgta
     7021 ttatctatga ttaaaaaatt ggttgagatt aaacgggaac ggatgggtac gacagcaaat
     7081 atgcagaaaa taatttttaa gatgtccata gggataaagg gaaatacagc gatcgctaaa
     7141 gtagcataaa aagagttgcc tgtggtgatg ctcagccagg tggttcctcc taaatataaa
     7201 atgatatgtg cagtgcaaat ggcaacagcg gatttaaaga gtaaaaatcc cttactgtta
     7261 gaggattcgt tttgcctgct tttttggctg ttaagcacca tagagacaag ccaggccata
     7321 atgggataga ccagtaagta gccgcctgtt ggtccaaaaa gggctcccag gccgccacga
     7381 aagccgccaa agactggaat tcccagacca cccagagtaa gataacacag ttgagaatag
     7441 acagcatgct ttggttttaa taaaattcct gacatgtaaa cggcaaccag accaaaggag
     7501 ataggtactg gtgtaaaagg gagcgggata gcgatttgtg caaatacagc agtgattgca
     7561 gtaaagagtg caatgactga aagttctttt gcagataaaa cagattttgc ttttttattt
     7621 ttcatcgaat aatcctcctt atatttcaat tgttaaccaa aaacattata tacttaacaa
     7681 ttgacattgt caaccataaa aaataagaga ttgacaattt agcttactat aaaagattat
     7741 ggatataaat tagaaaacga acaaagagat ttttactgaa taaaattttg aatggattat
     7801 gctaattata agatattgca tgaaaatatg agatttgtga tgaagaagtg gaaatgaatg
     7861 atgcttttaa ttaaaagttc agaggtgaca gataaagcat agacagagat tatttattga
     7921 caaattaaat atattgtatc ataattaaaa atattttgat ataaataaca ggctaataaa
     7981 agctacgtat tgatagcagc aagaatcaga taaagcgttg tcatttatgg agctaaatat
     8041 ttgcggtgat tgtctatgaa aaagaagagt ataataattg ggttttttat tatggtaatg
     8101 atttgtttat tgttttttat taattttata cctacttttc atttaaagtc ggcagatatg
     8161 agtcttctgg aaggtcaatg ggtgaatgta tattacgaaa aggaaaagga tgcagcaaca
     8221 gatactttta actatgcaga tactgcaaca gagtttatcg ctaaaaagtt aggctttact
     8281 gaaaaagaga atataaatat ttatatttat gattcacagg cgactatgca aagaaagaag
     8341 tacggattta ttggtccgct tttaggcctg gactggtata taggggacaa tattggaacc
     8401 aatgttattt taacctctcc tgctaatcct ggggccatgc ataactatga tagtgtgaag
     8461 tatgcagtgt tgcatgaaat tgtgcatgcc tatatcagtg ttttaaatcc ggacattgat
     8521 ttatggttaa cagaaggagt agcattgtat ttagctaatg gtgaagaatt ttataaagaa
     8581 tatttagaga caatgtctat tcctagttat agtgatatct gtacaagaaa tcctgtccgg
     8641 ttttcaaatt gcggcggtta tacttttgca acaacctatg ttgaatatct ggatgtaact
     8701 tatggatggg acagtgtgct tgaacttata aaaacagaag attatatgct ctgttttggt
     8761 aaatcacaag aagatattta tcaagaatgg gtagaatata taaaaaatta ttatcaataa
     8821 aagtctcaac tggatttaaa ggattatatt tttgttattt agggggatta gtatgataac
     8881 ttatggcaat aagatttctg aaaaagagta taatgattta agaaaggctg ttggatggaa
     8941 tacgctttcg aaagaacagg cggaaagagg acttatgaat tcggcttttc ttacagtagc
     9001 atataaagat gggaaggctg taggaatggc gagagtcgtt ggagatggcg gctatattgt
     9061 cattattgca gatgttatgg tattacctga gtttcagggg caaggaattg ggagacagtt
     9121 aatgaataat gtcatggact atttaaagaa ttcgatgcag aaaaatgatt ttctaatggt
//
LOCUS       DAOSUD010000078         8977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4127_length_8977_cov_26.031271, whole genome shotgun sequence.
ACCESSION   DAOSUD010000078 DAOSUD010000000
VERSION     DAOSUD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4127_length_8977_cov_26.031271"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..491
                     /locus_tag="PLG49_08985"
     CDS             192..491
                     /locus_tag="PLG49_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17485.1"
                     /translation="MKKYALLLFIMILTIILSVGCSSLNTAHWEEGIYETVNNLEGVR
                     MLVKEDSLSSKGLTLIIKNDSDRDCIFSEDFLLEKNRWKMAENYTHYCLAAKFNI"
     gene            complement(574..1224)
                     /gene="sigH"
                     /locus_tag="PLG49_08990"
     CDS             complement(574..1224)
                     /gene="sigH"
                     /locus_tag="PLG49_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOQ17486.1"
                     /translation="MSLKLKKTSRFVSYDEMEDEAIVDLVRDGNNDAMDYLINKYKNY
                     VRAKARTYFLIGADREDIIQEGMIGLYKAIRDYDEEKISSFRAFAELCITRQIITAIK
                     AATRQKHIPLNSYISLHKPIFNDGSEKTTLMDVIIGGKVANPEELLIGQENLEFIEQQ
                     MGEILSDLECKVLSLYLQGKAYYEIAEEMNRDIKSIDNALQRVKKKLEKFLNNKKG"
     gene            complement(1417..2172)
                     /gene="rlmB"
                     /locus_tag="PLG49_08995"
     CDS             complement(1417..2172)
                     /gene="rlmB"
                     /locus_tag="PLG49_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOQ17487.1"
                     /translation="MKNFNEDALRLEGRNAVLEALKSERDIDKILVQEGSKEGSIKKI
                     LAEARKRKIVIHTVSKAKLDEISETKKHQGVIAFVSAHNYVELEDIIKSAEEKGEKPF
                     IIILDSIEDPHNLGAIIRTANICGAHGVVIPKRRAVGLTATVAKASAGAIEYIPIAKV
                     TNIGSAIDKLKSLGIWVACADMDGELMYKTDLTGAIALVIGSEGEGVSRMVKEKCDYI
                     VKIPMYGQITSLNASVAAGILMYEVVRQNKFKE"
     gene            complement(2337..3959)
                     /locus_tag="PLG49_09000"
     CDS             complement(2337..3959)
                     /locus_tag="PLG49_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOQ17488.1"
                     /translation="MSKETKTFIKGAIILSIGGVIAKILSAFFRIPLTHLIKDTGIGY
                     YQMPYPIYTLMIAIAYTGIPSTVSKIVSERLVHKKYKEAHRIFQYTFIVLIFTGVLAS
                     LFLFLGADWLIYMQGWVPEAKYSLLGLALSPIFIAIMGAFRGYFQGMQNMFPTATSQI
                     FESFARVIFGLGLAYYFMSSNFGIAVAAGGASFGATAGAIFGTLILLIYYLTKRKKII
                     EKVHEDNESNQEVEFLPTAARILWIAFPVSVGAAVNSVMNWADSALVVRRLIHAGATQ
                     LEATDLFGQIGKASTFINIPLTFSMALVVGLVPSIAEAIEKRNKKELHDKIEWGTRFA
                     MLLGLPSAAGLGILAQPLMTLIYWEYNKGASILALLSISLVFIILGQAYTGVLQGMGK
                     FYIPVVNLLIAVLCKALVNYTLVAGPLGVNGAAIGSIIGYGVFAILNYIFVKKYSDFK
                     MNLKLVIFKPLLSTLLMAGVTFGVYYLFRNFTGNTISTLTAVACGVIIYALMLLLTGA
                     IAEEDFHMIPQGDKLLWLLKKVKIIKVQSRSL"
     gene            complement(3980..4417)
                     /locus_tag="PLG49_09005"
     CDS             complement(3980..4417)
                     /locus_tag="PLG49_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOQ17489.1"
                     /translation="MDDQFITNQYLNALFEHVKSNTVKPREYSPLVLAYIGDAVFEIF
                     IRTAIVVKGNAPVNKLHKAAKEYVKASAQAEIYEKIKSCLTEEEESIFKRGRNAKSAT
                     VPKNALLSDYRHATGLEALFGYLYLDGQIERLQYLMNLGIEKE"
     gene            complement(4443..5849)
                     /gene="cysS"
                     /locus_tag="PLG49_09010"
     CDS             complement(4443..5849)
                     /gene="cysS"
                     /locus_tag="PLG49_09010"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ17490.1"
                     /translation="MKIYNTLTRKKEEFIPVDPGEIKMYVCGPTVYNYIHIGNARPYI
                     IFDTVRRYFEYKGYQVKYVQNFTDVDDKIIKRAQEEKTTTTEIVEKYIAEALKDADGL
                     NIKRATVHPRVTEEIPEIIDMIKTLIEKGFAYEVNGTVYFDTQAFDSYGKLSKKNQEE
                     LEAGARIEVNEDKKHPMDFVLWKPNKEGEPYWESPWGQGRPGWHIECSAMARKYLGDT
                     IDIHAGGEDLIFPHHENEIAQSEAATGKEFARYWMHNGFINVDNKKMSKSKGNFFTLR
                     EVAQEFPYEVIRLFMLSAHYRSPINFSRELMQAAQNSLERIKNALFNLDHLIRHSQEE
                     KMTEDEKRLEKELEHFQNKFEEAMEDDFNTADAVSVIFELVKFSNVNVDSNSSKEFAI
                     AVKNKILALLNILGLFEKEEDTADILDEEIEELIMKRQEARKAKNWALADEIRDHLKQ
                     KGIILEDTPQGVKWKRQG"
     gene            complement(5836..6501)
                     /gene="cysE"
                     /locus_tag="PLG49_09015"
     CDS             complement(5836..6501)
                     /gene="cysE"
                     /locus_tag="PLG49_09015"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOQ17491.1"
                     /translation="MLGFIREEIRVIKERDPAIKKTIEVFLYPSFYAILSHRIAHWFY
                     NHKRYFIARFISQISRGFTGIEIHPGAKIGKGLFIDHGMGVVIGETCEIGDNVTIYQG
                     VTLGGTGKEKGKRHPTIGNNVMIGAGAKILGPFKIGDNSRIGAGSVVLTEIPENCTCV
                     GIPARIVRRDKQKVDPCQSLDQVKIPDPVQMELCKLQVKIRELEKEIEELQNKGGKGN
                     EDI"
     misc_binding    complement(6540..6752)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6821..8218)
                     /locus_tag="PLG49_09020"
     CDS             complement(6821..8218)
                     /locus_tag="PLG49_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ17492.1"
                     /translation="MPKEELDCLLEEILSINEHFKRAKADDHVLIQGNYTGKISMLKD
                     AFNWMLNTIYEQEAKLQEDRIEIGRYSRQIGHKENELYRRQSELEKAHLQLKAYTKEI
                     ESINAELNRKVEELKYLFYFSQEVTSTFERYELLKIALRDIVPFIDGSFGAAFISNEA
                     GLELVDTYYKDTNVFLKVNAEINRLLNKNYLVYELSPNKVEIYYDINQDSRIKRIIGD
                     DLAQFFRSLAIAPIVNKDDEIVAVIFLLGDYFDDSEKYLLSCYTNVLRIAFENAGLYN
                     DINKMFIDTVKVLANAIEVKDRYTKGHVDRVTDYSVAIARKMNFDKSSLEKIKIAAAL
                     HDIGKIGVPDEILNKPAKLTKEEFHIVKQHPIKGYEIIKDIPALKDISIYVKQHHERI
                     DGRGYPDGLKNHEITLEARIITVADAFDAMTSDRPYRKGMSLNTAVKILQENKGSQFD
                     EYVVNVFLSCLSELR"
     gene            complement(8387..8854)
                     /gene="ispF"
                     /locus_tag="PLG49_09025"
                     /pseudo
     CDS             complement(8387..8854)
                     /gene="ispF"
                     /locus_tag="PLG49_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
BASE COUNT         2743 a         1770 c         1203 g         3261 t
ORIGIN      
        1 aggctttttc ttaagtttaa tgctggaaaa tatgataaaa gttagatgct gtttaatctt
       61 ttttactaaa aaaacatact ttaaggattg acaattgcaa agatatcaaa aaaacggata
      121 aatttctata atcgttttct tccggaaact tatgttttat cccagcatct aataggataa
      181 ggagtagtta tatgaaaaaa tatgcgcttt tactttttat tatgatttta acgattattt
      241 tatcagtagg atgcagttca ttaaatactg cacattggga agaaggcata tatgaaacag
      301 tcaataatct tgaaggagtc cgtatgcttg ttaaagaaga cagtctgtct tctaaaggat
      361 taactttaat aattaaaaat gatagtgaca gggattgtat ctttagtgaa gattttcttt
      421 tagaaaaaaa tagatggaaa atggcagaga actacactca ttattgtctg gcggctaaat
      481 ttaatattta aaagcaattg aaacagattt aagtatttta gaaaatatat tgaaaggata
      541 ataaaaacat cagaattata actgatgttt tcattatcct tttttattgt taagaaattt
      601 ttctaatttc ttctttactc tttgcagcgc attatctatg gattttatat ccctattcat
      661 ttcttctgct atttcataat aggcttttcc ctgcaaatag agagataaaa ccttacattc
      721 caaatcactt aaaatttctc ccatctgttg ttcaataaat tctaagtttt cttgtcctat
      781 aagcagttct tcgggatttg ccactttacc ccctataatc acatccatga gggttgtctt
      841 ttcactgccg tcattaaaaa ttggtttatg caaagaaata tatgaattca ggggaatatg
      901 cttttggcga gttgcagctt ttatggccgt aattatttga cgggtaatac acaattcagc
      961 aaaggcacga aaagatgaaa ttttttcttc atcataatct cttatggcct tatataaacc
     1021 tatcattcct tcttgaatga tatcttccct gtcagctccg attaaaaaat atgttctggc
     1081 ttttgcacga acataatttt tatatttgtt gattaagtaa tccatggcat cattgttgcc
     1141 atctcttact aaatcaacaa ttgcttcatc ttccatttca tcataagata cgaatctact
     1201 ggtctttttt aattttaagc tcattgttcc cgccccccgt tctgtttctt taatgctttt
     1261 gtgtattatt tagtttactg ctattactgc tgtacagaaa aaagaaaaat ttcattaagc
     1321 agcttgtcct ttctgctctc tataaattag tataacacaa tatatttatt ttgttaaggg
     1381 aaactgtaaa atataaccat ttgcatcgct tacattttat tccttaaatt tattttgcct
     1441 tacaacttca tacattaata tgcccgctgc tacagaagca tttaaagaag taatttgtcc
     1501 atacatcggg atcttgacaa tatagtcgca tttttctttt accattctgc tgacaccttc
     1561 cccttcactg ccaatcacta aagcaatggc tcctgttaaa tcagtcttat acatcagttc
     1621 tccatccata tctgcacagg caacccatat acccagagat ttaagtttgt ctattgcgct
     1681 tcctatattg gttacttttg caatagggat atattcaata gctccagcag atgcctttgc
     1741 cactgtcgct gttaaaccta ctgctcttcg tttggggata actaccccat gagcaccaca
     1801 aatattggct gttctaataa tggcacctaa attatgagga tcttcaatgg agtctaaaat
     1861 aataataaaa ggtttttctc ctttttcttc ggcactcttt ataatatcct caagttctac
     1921 ataattatga gctgatacaa aagcgataac cccttgatgt tttttagttt cagatatttc
     1981 atctaatttt gcttttgaaa ccgtatggat gacaattttt cttttcctgg cttcggcaag
     2041 gattttttta atagaacctt ccttacttcc ttcctgaact aaaattttat caatatctct
     2101 ttcagatttc aaggcttcta aaacagcatt tcttccttcc aaccgaagag catcttcatt
     2161 aaagtttttc ataaaaaaac tcctctcttc ttacgaatat gaatcataat aatattattt
     2221 tgagcctttt ttttaatata ctgttaaatt tttatataaa atatttattc gatgataccc
     2281 gaaacttcaa aaatccagaa gtaagaaaga tctgatatat ttaatgaaga taattattac
     2341 aaagatctgc tctgaacttt aataattttt actttcttta atagccagag gagcttatca
     2401 ccctgaggaa tcatatggaa gtcttcttct gcaattgcac ctgttaataa aagcataaga
     2461 gcataaataa taacaccaca ggcaacagca gttaaagttg aaatcgtatt acctgtaaag
     2521 tttctgaaca gataatatac cccaaaagtt acccctgcca ttaaaagtgt tgacaataat
     2581 ggtttgaaaa ttactagctt aagattcatc ttgaagtctg aatacttttt aacaaaaata
     2641 tagtttaaga ttgcaaaaac tccataacct ataatagaac ctattgctgc accgtttact
     2701 cctaatggtc ctgcaaccag ggtataatta acaagagcct tacataaaac tgcaattagc
     2761 aaattaacta cgggaatata aaatttaccc attccttgta aaacacctgt ataggcctgc
     2821 cctaatatga taaagactaa gcttatactc aataatgcaa gaatgctggc acctttatta
     2881 tattcccaat aaataagtgt cattaagggt tgagccagga tgcctaaacc agctgctgaa
     2941 ggcaaaccaa gtagcatagc aaatctggtt ccccactcga ttttatcgtg aagttctttt
     3001 ttatttcttt tttcaatggc ttctgcgata cttggtacca atcccactac taaagccata
     3061 ctaaaagtta aagggatatt tataaaagtt gaagcctttc caatttgtcc aaataaatcg
     3121 gttgcttcta attgagtggc tccggcatga atcaatcttc ttaccaccaa tgctgaatca
     3181 gcccaattca taaccgaatt aaccgcagct cctacggaaa cgggaaaggc aatccataaa
     3241 atccttgctg ccgttggtaa gaactctact tcttgattac tttcattgtc ttcatgaact
     3301 ttttctataa tttttttcct ttttgttaaa taatatatca ataaaataag agtaccaaaa
     3361 attgctcctg ctgttgcacc aaaagatgct cctccagcag caacggctat accaaaatta
     3421 ctgctcataa agtaataagc cagacctaaa ccaaaaatga ctcttgcaaa actttcaaaa
     3481 atttgtgatg tggccgtggg aaacatattt tgcataccct gaaaatatcc tctgaaagcc
     3541 cccatgatag caataaaaat cggactaaga gccagtccca ataaagaata ttttgcttca
     3601 ggaacccaac cctgcatata gattaaccag tctgccccta aaaatagaaa taaggatgca
     3661 agcactcctg taaagattaa aacaataaag gtgtactgga atattctatg ggcttctttg
     3721 tattttttat gaaccagcct ttcagaaacg atttttgata cggtagaagg aattcctgta
     3781 taagcaattg ctatcatcag tgtataaatt ggataaggca tttgataata acctattcct
     3841 gtatctttaa tcaaatgggt caatggaatt ctaaaaaacg cactcaatat ttttgcaatt
     3901 actcccccta tagaaagaat aattgccccc ttaataaatg tttttgtctc tttagacatt
     3961 tgttgtaacc tccatgtctt tattcttttt ctatccccaa attcattaaa tattgaagtc
     4021 tttctatctg tccatctaaa tacaaataac caaaaagggc ttctaaacct gtcgcatgcc
     4081 tgtaatctga cagaagggca tttttgggaa cagtagctga ttttgcattt cttcctcttt
     4141 taaaaatgct ttcttcttct tcggtcagac aggatttgat tttttcatat atttcagcct
     4201 gggcagaggc ctttacatat tcctttgccg ctttatgaag tttattaaca ggtgcatttc
     4261 cctttacaac aatagccgtt ctgataaaaa tttcaaaaac tgcatctcca atatacgcca
     4321 aaaccaaggg cgaatactcc cttggtttga cagtattact ttttacatgc tcaaaaagag
     4381 catttaaata ctgattcgta atgaattgat catccataca gatctccgtt tcttttataa
     4441 atttagcctt gtcttttcca ttttactccc tgaggagtat cttctaaaat gatacctttt
     4501 tgctttaaat gatctcttat ttcatccgct aaagcccaat tctttgcttt tctggcttcc
     4561 tgacgcttca tgataagttc ttctatctct tcatccaaaa tgtcagcggt atcttcttct
     4621 ttttcaaata agcctaaaat atttaacaag gccagaattt tatttttgac agctatggca
     4681 aattcttttg aagaattact atctacattg acatttgaaa acttaaccag ttcaaagata
     4741 accgatacag catccgctgt attaaaatca tcttccatag cttcttcaaa tttattctga
     4801 aaatgttcta attccttttc taagcgcttt tcatcttctg tcattttttc ttcctggcta
     4861 tgacgaataa gatgatccag attaaacaga gcatttttaa ttctttccag actgttttgg
     4921 gcagcctgca ttagttctct gctaaaatta atgggacttc tgtaatgtgc acttaacata
     4981 aaaagacgga ttacctcata aggaaactct tgtgccactt cccttaaggt aaagaagtta
     5041 cctttggatt ttgacatttt tttattatct acgtttataa aaccattatg catccaatat
     5101 cttgcaaatt cttttcctgt agctgcttca gattgggcaa tttcattttc atggtgcgga
     5161 aatattaaat cttctcctcc tgcatgaata tcaatggtat cacctaaata tttccttgcc
     5221 atggcagaac attcaatatg ccagcctgga cgtccttgac cccaggggct ttcccagtaa
     5281 ggttctcctt ctttattagg cttccataga acaaaatcca taggatgctt tttatcttca
     5341 ttcacttcaa ttcttgcgcc tgcttctaat tcttcctgat ttttctttga taattttcca
     5401 taggaatcga aagcctgggt atcaaaataa accgttccat tgacttcata agcaaagcct
     5461 ttttcgatta aagttttaat catatcaatg atttctggga tttcttctgt taccctggga
     5521 tgaaccgtgg cccttttaat attaagacca tcagcatcct ttaaagcttc agcaatatat
     5581 ttttccacga tttctgtggt tgttgttttt tcttcctgag ctcttttgat gattttatca
     5641 tctacatctg taaagttttg aacatatttt acctgatagc ctttatattc aaaatatctt
     5701 cttacggtat caaatataat ataaggtctt gcatttccta tatgaatata attatacacg
     5761 gtaggcccac atacgtacat ctttatttca ccgggatcta caggaataaa ttcttctttt
     5821 ttccttgtca gcgtgttata tatcttcatt gccctttcct cctttatttt gaagctcttc
     5881 aatttctttc tctaattctc ttatttttac ctgaagtttg catagttcca tttgtacagg
     5941 gtctggtatt ttaacctggt ctaacgattg acaaggatct actttttgct tatctcttct
     6001 tacaatcctt gctggaatcc ctacacaagt acaattttca ggtatttcag tcaatacgac
     6061 agaaccagcc ccgattctgg aattatcccc tattttaaaa ggacctaata ttttagcccc
     6121 tgcacctatc ataacattat ttccaatcgt aggatgtctt ttaccttttt cttttccagt
     6181 accacctaaa gtaactccct ggtaaatggt cacattatcg cctatttcac aagtttcacc
     6241 aatcacgaca cccattccat gatcgataaa taaaccttta cctatttttg ctcccggatg
     6301 aatttctatt cccgtaaatc ctctggatat ttgggaaatg aatcttgcga taaaatagcg
     6361 tttatggtta taaaaccaat gagctattct atgcgataaa atagcgtaaa agctagggta
     6421 taagaaaacc tctattgttt ttttaatcgc tgggtctctt tcttttatca ctcgaatctc
     6481 ttctcttata aagcccaaca taataatctc ctttcaacca ataaaaaact ccatctcaat
     6541 tcagagacga agttaatccg cggttccact ctgcttaaag cttacgcttt ccctcaatgc
     6601 atataacggc atgccaccgg aataaactac taaaatttca tctattctac tccaggatgc
     6661 acttcattta tatgcgcttt aaaccgctct cagctaatac ggttttctct gtataaaggc
     6721 atataaacta ctcttccctt catcgtattt aacattgtac tttttgatat tataacagat
     6781 ttacaaagca ttaatcaatt ctattttaat atcttatttt ttatctaagt tcagataaac
     6841 aagatagaaa tacatttacc acgtactcat caaattgact gcctttattc tcttgcaata
     6901 ttttaactgc tgtattcagg ctcatgcctt tacggtaagg cctatctgaa gtcatcgcat
     6961 caaaggcatc cgccacagtt ataatcctgg cttccaaagt aatctcatga ttttttaagc
     7021 catccggata gcctctgcca tcaattcttt catgatgttg cttgacataa atactaatgt
     7081 cctttaaagc tggtatatcc tttataattt cataaccttt tatgggatgt tgctttacga
     7141 tatgaaattc ttctttagtt aatttggcag gcttatttaa tatttcatca ggaacaccta
     7201 ttttccctat atcatgcagg gctgcggcaa tttttatttt ttctaaacta cttttatcaa
     7261 aattcatctt tctggcaata gcaacggaat aatctgttac tctgtcaaca tgccctttag
     7321 tatacctgtc ttttacttct attgcattgg caagaacctt tactgtatca ataaacattt
     7381 tattgatgtc attataaaga ccagcatttt caaaagcaat tcttaaaaca tttgtgtagc
     7441 aactgagtaa atatttttcg ctgtcatcaa aataatctcc taataaaaat attacggcaa
     7501 cgatttcatc atctttattc acaatgggtg caattgccaa agatctaaaa aactgggcta
     7561 agtcatcccc aattatcctc ttaattctac tgtcttgatt gatgtcataa tatatttcta
     7621 ctttattggg agaaagttca taaactaaat aattcttgtt gagcaatcgg ttaatttcag
     7681 cattaacttt taagaaaaca tttgtatctt tataataagt gtctacaagt tctaatccag
     7741 cttcattaga aataaaagct gcaccaaaag aaccatcaat aaagggtaca atatctctta
     7801 aagcaatttt taaaagttcg tatctttcaa aagttgatgt gacttcctga ctaaaataaa
     7861 ataaatactt taattcttct acttttctgt ttaattccgc attaatgctt tctatttctt
     7921 tagtatatgc ctttaattgt aaatgggcct tttctaattc tgattgtctg cgatataatt
     7981 cattttcttt gtgacctatt tgcctggaat atctgcctat ttcaatcctg tcttcctgaa
     8041 gtttagcttc ttgttcataa attgtattaa gcatccagtt aaaagcatct tttaacatgg
     8101 agattttacc agtataattt ccctggatca atacatggtc atcagcctta gctcttttaa
     8161 aatgctcatt aatcgacagt atttcttcta atagacaatc caattcttct ttaggcatac
     8221 ttgtcacctc aaatatttac aattcattag taatccattt acccataaat acaattcgct
     8281 aaagctttgt aataaattaa attttctaaa aataacaaaa attttaaagt atagtatttt
     8341 aattttatca ctgcaagttt tacttttcaa tgggaaagaa gattcatttt tttaacatac
     8401 agattgcata agcagatatg cctttgcctt ctcctgtaaa ccccaaacct tcttctgttg
     8461 tagcttttat attgatttgt tctatttcaa tttctaaaac ttctgcaaca ttctttctca
     8521 tactatcaat atatgatgcc agtttaggcc tttgtgctac gattgttgaa tcaatattaa
     8581 caactgtata accatcctca tttaatatgt ctttaactct ctttaataaa atcatactgg
     8641 aaatatcctt atatttttca tcattatcag gaaaattctt tcctatatct cctttagctg
     8701 ctgcccccaa aagtgcatcc attatcgcat gaataagaac atctgcatca gaatggccca
     8761 taagcccttt ctcataagga atctctatac cacctaaaat taattttctg ttttctgtca
     8821 gtttatgtac atcataacct attcccactc tcactggtct tcctcctatt tttatcaaag
     8881 aaaaatttat tttttcagtt attttataca tttgttgtac acaagattcg ttcatatttc
     8941 ataatttttt ctatacatca gtaaattttt atactaa
//
LOCUS       DAOSUD010000079         8829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4200_length_8829_cov_23.584682, whole genome shotgun sequence.
ACCESSION   DAOSUD010000079 DAOSUD010000000
VERSION     DAOSUD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_8829_cov_23.584682"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLG49_09030"
     CDS             complement(<1..417)
                     /locus_tag="PLG49_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17493.1"
                     /translation="MKGFRLRFRGKMVALLAVLLIVFGIALYLFISNGISQLNNYNMK
                     RQLESILDLEYELFSLKFEGEWTLKDGALYKGNVLLEDNSELADVVSGASRYSVALST
                     KEKVVASSINDSAISPELLKQLEESKQNVMDEVEVNG"
     gene            complement(806..889)
                     /locus_tag="PLG49_09035"
     tRNA            complement(806..889)
                     /locus_tag="PLG49_09035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Leu,seq:caa)
     gene            complement(893..967)
                     /locus_tag="PLG49_09040"
     tRNA            complement(893..967)
                     /locus_tag="PLG49_09040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(933..935),aa:Gln,seq:ctg)
     gene            complement(1079..1924)
                     /locus_tag="PLG49_09045"
     CDS             complement(1079..1924)
                     /locus_tag="PLG49_09045"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOQ17494.1"
                     /translation="MKKEIIIAKYSGFCFGVKRAVDMTTNHKNTNGKIYTLGPLIHNK
                     DVINRLNNQGIKIMEMDEFDKLTEEDTLVIRSHGVGAEIIQKLENSGANIIDATCPFV
                     SSIHRKVKKYSDLGYQIIIVGDKDHPEVIGINGWCDNTAIVTKDGTDLKDIPSRVCIV
                     AQTTERLTNYEKVVDIVSKNCKELVKFNTICNATKERQESAQELSKEVDLMIVIGGKN
                     SSNTKKLYEICRLNCENTLFIENSTELPKWVITDETIKKIGITAGASTPDWVIEEVVN
                     KIKNE"
     gene            complement(1978..3426)
                     /locus_tag="PLG49_09050"
     CDS             complement(1978..3426)
                     /locus_tag="PLG49_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOQ17495.1"
                     /translation="MNFRMIRRILGILLIIEAIFMIPPFLISVYYGQQDMYAFLLSIL
                     FTGITGFTMYKLKVKNTTIKVRDALAIATFGWLIISFFGALPFVFSRSIPSLVDAFFE
                     TVSGFTTTGATLVNHLEDMPKSILFWRSFTHWIGGMGILVFTVAFLPAMGVGSFQIFK
                     AESPGPTADRLVPRIKDSAKLLYMTYFSFTIVQIIFLLFGKMSLFEAVVYTFGTVGTG
                     GFATEATSIAAYDSVYIHIVIAIFMFLSGINYSLYYSLFKGKWRDVVKNQEFKLYLGI
                     IFVSTILITVNLMTTIYHDIGTAFKDAFFQVNAIITTTGYAIADFDLWPAFSKAVLFM
                     LMFVGGCAGSTAGGIKVIRILTLLKLIKREIQRIFHPRAQIAIKNGENIIQNETASSI
                     TSFCALYFLIFILSIVIVSLDGFDLETTISAVATTLGNVGPGFNQVGPMRTFSVFSAF
                     SKITFSILMLLGRLELFTMIAIFSPEAWKKQA"
     gene            complement(3423..4814)
                     /gene="trkA"
                     /locus_tag="PLG49_09055"
     CDS             complement(3423..4814)
                     /gene="trkA"
                     /locus_tag="PLG49_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOQ17496.1"
                     /translation="MNEDQKGRGAMKVIIVGVGKLGYKLAETLANTDVDVTVVDQDEK
                     VLEGINNYLDVLTVCANGIEIEVLKEINIRSYDLLVATTESDETNALICTLAKRLGCK
                     RTIARIRNPEYTKQVEFIKSELGIDHIINPDLATSNEIARYLLKSYNFYSEDFAKGKV
                     SIVDFNVNQIPELIGKKIMDLDIFEDLLIVAIFRDGELIIPYGNIELQEDDILNIIGS
                     LEAINKFSERFHFINQRNKIKKVMILGGGNIAFYLVQKLRYNHIDVRIIEQDLERSTY
                     LSEKLSDVLIIQGDGTDINLLEEEGLGSMDAFIGATGFDEQNLLMALIAKHAGVKMTI
                     AKVSRPSYLKIIDSLDVDVFLNPINIAVSNILKFIRGGKVVSVSLLLGGEAEVIEMIA
                     LKDSRIVGKPLYQLGLPKGIIIGAIVHEGKVSIPNGNTVIYPNDRLIIFCLASDIPKL
                     EAFTNPEKGGLFR"
     gene            complement(4869..5945)
                     /locus_tag="PLG49_09060"
     CDS             complement(4869..5945)
                     /locus_tag="PLG49_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOQ17497.1"
                     /translation="MKSLGNFDIKIEDILMWNILLALLIILLTIVLKRAIIKLLIKLL
                     NRLTKNSKGNWMNKLFMVLDKPLELAFVTFAFYIALKLISFPVNIGLVINNIAKTIVL
                     FTIFWAAYRIANVFDSLYEVITEKTDKKLDGMVLSFLKNGIKIVIVIIGSITILQVWF
                     KELGGLLAGLGLGGLAFALAAQETAANLFGGIAIMSDRPFEIGDWIQTPSVEGTVEEI
                     GFRSTRIRTVQQTLVTIPNSVLSKEVIRNWSKMGKRLISYRLGLVYNTGLEQLKACLE
                     EIRKMLKEHPEVHPETIYVYFERFGESSLDIFIHFFTKATERQKYLEVQEDVNLKILE
                     ILNKLEISIAFPSRTIYVEKTDKK"
     gene            6143..7597
                     /locus_tag="PLG49_09065"
     CDS             6143..7597
                     /locus_tag="PLG49_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ17498.1"
                     /translation="MNITVLRNHIHKVNKAMSYFLIFLAVTALVSSFVLRMPILLANF
                     AIFLFSSIFMIYSYRKQKLEMVSSYILSFSLVLLFVLPLPSNEYLFIAIIPVIVASMY
                     FNQKLVIITSTVLNISILAKELLAGNINSYFITTLLIIDFTILILLTLSLIGNRLIHL
                     ANAEIIKSNNLLSQLNETMEIIQKTSENLSKDIQNSNLNLELIKRNGDTVTHSVQEIS
                     KGIIEQTESVSRISEMMNDADKRVEEVNELSKQLADISTETSETVREGSEKINNMAHQ
                     MDIINQAVTKSYATVLELNENMDAVNSFLTSIAQISEQTNLLALNAAIEAARAGESGK
                     GFAVVAEEVRKLAEQSATTTEQISEIIINIQEKSRDVLEEVQNGTLATNEGEKIAKAV
                     NENFEKINQSFKKIDEYIYEELTKNQNISSLFSQIRAETESIATISEEHSAATEELMA
                     TTEEHNASIETIYNAMLDIQKACENLQNIVNNNR"
     gene            complement(7661..8635)
                     /locus_tag="PLG49_09070"
     CDS             complement(7661..8635)
                     /locus_tag="PLG49_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOQ17499.1"
                     /translation="MLYPLKMNPIYKEAIWGGEKLRKVFGKAIPSCHTGESWEIACHQ
                     NGMSLVANGELEGKSLKEVLDIYGEEALGTEIWKRKYGKFPLLVKIIDASDKLSVQVH
                     PQDEYAKIHEGDLGKTEMWIVLDAKPDAKLIYGLKAGISKKDFEIAIEEGSLEDVLNY
                     VDVKKGDVFFIPAGTVHAIGEGILIAEIQQNSDTTYRVYDWNRTDENGNSRELHIKKA
                     LDVISFSNLAGKEKVVGKLVKEGKNTRNYLISCEYFSTELLEIHTESKESLNGERFNL
                     LIFIEGEGSLEYKNGYISFKAGDSFFIPASIGDYFIVGPAKVIKTYIE"
BASE COUNT         3018 a         1723 c         1127 g         2961 t
ORIGIN      
        1 gccattcact tcaacttcgt ccataacatt ttgcttactt tcttctaatt gcttcaatag
       61 ttcaggagat atggcagaat cattaattga agaagcgact actttttctt ttgtagacaa
      121 ggctacagaa tatctgcttg cacctgacac aacatctgca agctcactat tatcttccag
      181 caaaacatta cctttatata gagctccgtc ttttaatgtc cattcccctt caaattttaa
      241 agaaaaaagt tcatattcca aatctaaaat agattctaat tgtcttttca tgttataatt
      301 gtttaattgg cttatcccat tactgatgaa aagataaagc gctataccaa agacaattaa
      361 cagaactgcc aataaagcca ccatttttcc tctaaacctg agccggaaac ctttcataca
      421 attcctcctt tttattacta ttggaactat tattttttaa aagtgataat aaatagttat
      481 aaaatatcat tcgcttataa taataatgtc taaatatttt attagaatta tatcatattt
      541 ctaaaatgtt aactatcata aaacaaataa attaacactt tttatttaaa tttcatttta
      601 ctttttcagg tcctgaaaaa ctttatttta tccttgtttt tgtaaataaa cagcttattt
      661 aaacttatca tttcactgct atgtcatctg tttattgtct ttcccgaata aaaataaaaa
      721 agcaagaaaa gaaacatgta accgtcccct tattttccta aaaaaaagaa gcaagaaaat
      781 cattcccttg cttcctttga caacatgcgg atgaagggac tcgaaccccc acgatgtcgc
      841 caccggcaga ttttgagtct gctgcgtctg ccaattccgc cacatccgca tatggctggg
      901 taggaaggat tcgaaccttc gcatccagga gtcagaatcc tgtgccttac cgcttggcca
      961 ctacccaaaa aaatatccga acaacaaaaa attttatcat gaatttactc atttgtccag
     1021 tgatttaatt ttccttttta ataaatttat tatttcttaa tcaactagct taaaattttt
     1081 attcattttt tattttatta acaacctctt caatgaccca atcaggtgtt gaagctccgg
     1141 cagtaatgcc tattttctta attgtttcat ctgtaatcac ccactttggt aattcagtgc
     1201 tattttcaat aaaaagagta ttttcacagt tcaatcggca tatttcatat aacttttttg
     1261 tattggaact gttttttcct ccaatcacta tcattaaatc aacttctttt gatagctcct
     1321 gagcactttc ttgtctttct ttcgtggcat tacatatggt attaaattta accagttctt
     1381 tgcagttttt tgaaacaata tctacaacct tttcataatt tgtgagcctt tctgtcgttt
     1441 gagccacaat gcataccctt gaaggaatat cttttaaatc tgttccatcc ttagtaacta
     1501 tagctgtatt atcacaccag ccgttaattc caatgacttc aggatgatcc ttgtctccta
     1561 caatgataat ttgatatccc aagtcagaat acttttttac ttttctatga atacttgaaa
     1621 caaaaggaca agtggcatca ataatattgg ctccactatt ttctagcttc tgaataattt
     1681 cagcacccac accatgcgat ctgatgacaa gggtatcctc ttcagtaagc ttgtcaaatt
     1741 cgtccatttc cattatctta ataccctgat tgtttaaacg attaatgaca tctttattgt
     1801 gaataagggg tcctaaggta tatatcttcc catttgtgtt tttatgatta gtagtcatgt
     1861 ctacagcccg ttttacacca aaacaaaagc ctgaatactt tgcaatgatt atctcttttt
     1921 tcataattat catactccta tatcaaataa accaataaaa ctttttacgc actattttta
     1981 tgcctgcttt ttccatgcct ctggactaaa aattgcaatc attgtaaata attcaagtct
     2041 tccaaggagc ataagaatag aaaaggttat tttactgaaa gcactaaata cactaaatgt
     2101 tctcattggc cctacctgat taaaaccagg acccacattt ccaagcgtcg ttgcaacggc
     2161 agaaattgtt gtttccaaat caaaaccatc cagagaaacg atcacaatgg ataatatgaa
     2221 aattagaaaa tataatgcac aaaaacttgt tatactcgat gctgtttcat tttgaataat
     2281 gttttcacca ttttttatag caatttgggc gcgaggatga aaaattcttt gaatttctct
     2341 ttttattaac tttaataaag tcaaaattct aataacctta atacctccag cagttgagcc
     2401 cgcacaacct cctacaaaca tcagcataaa taaaaccgct ttactaaaag caggccaaag
     2461 atcaaaatct gctatcgcat agccagtggt cgtaataata gcattaacct gaaaaaaagc
     2521 atctttaaaa gctgttccaa tatcatgata tatagttgtc attaagttaa cagttattaa
     2581 tattgttgaa acaaaaataa ttcctaaata taacttaaac tcctgatttt ttactacatc
     2641 tctccacttt cctttaaaca aagaataata taaagaataa ttaattcccg ataaaaacat
     2701 aaatatagca atgactatat gaatgtaaac gctgtcatat gctgctatac tagttgcctc
     2761 tgtagcaaag cccccagtac caactgtccc aaaagtataa acaaccgcct caaaaagact
     2821 cattttccca aaaaggagaa atataatttg tacaatcgta aaagaaaaat aggtcatata
     2881 taaaagtttt gctgaatctt ttattctcgg aaccaatctg tctgctgtag gccctgggct
     2941 ttcagcttta aaaatttgaa aactacctac acccattgct ggtaaaaaag ctactgtaaa
     3001 aacgagtatt cccataccac ctatccaatg cgtaaaagag cgccaaaaca aaatactctt
     3061 aggcatatcc tctaaatgat taactaaagt agcaccagtt gttgtaaaac ctgaaacagt
     3121 ttcaaaaaaa gcatcaacta aggaaggaat agatcttgaa aaaacaaagg gtaaagctcc
     3181 aaaaaatgat attataagcc agccaaatgt agcaatcgcc aaggcatctc tgacttttat
     3241 tgttgtattc tttaccttta atttatacat tgtaaaacca gtaataccag taaatagaat
     3301 ggacaaaaga aaagcataca tgtcctgttg cccataatat actgatatta aaaaaggagg
     3361 aatcataaat attgcttcaa taataagcaa gattcccaat atccttctta tcattctaaa
     3421 attcatctaa ataatccacc tttctcagga ttagtaaatg cttctaattt aggtatatct
     3481 gatgccaggc agaatattat taaccgatca ttgggataaa tgactgtatt cccattaggg
     3541 attgaaactt taccttcatg aacaatagca ccaattataa ttcctttagg taatcctaat
     3601 tgatataaag gtttcccgac tatcctggaa tccttaaggg ctatcatttc aataacttca
     3661 gcttctcctc ccaacaagag agatactgat acaacttttc ctcctcttat aaatttaaga
     3721 atattactaa cagcaatatt gataggattt aaaaatacat caacatccaa actgtcaatg
     3781 atcttgagat aacttggcct gctaacttta gcaatggtca tttttacacc tgcatgtttt
     3841 gcaatcaggg ccataagcag attctgctca tcaaaccctg tcgctcctat aaaggcatcc
     3901 atggaaccca aaccttcttc ttctaaaaga tttatgtcgg tcccatcccc ttgaataatt
     3961 aatacatcac ttaacttttc tgataaatag gtggatcttt ctaaatcttg ttcgattatt
     4021 cttacgtcaa tatgattgta tcttaacttt tgcactaaat aaaaggcaat atttcctcca
     4081 cctaaaatca ttactttttt tatcttattt ctttggttaa taaaatgaaa tctttcacta
     4141 aatttattaa tggcttcaag actaccaata atattaagaa tatcatcttc ctgtaactca
     4201 atattcccat agggtatgat aagctcccca tctctaaata ttgctacaat aagtaaatct
     4261 tcaaatatat ccagatccat tatttttttg ccaatgagtt ccgggatctg attaacgtta
     4321 aaatcaacaa ttgaaacttt tcctttagca aaatcctcgg aataaaaatt atagctttta
     4381 agaagatatc tggctatttc atttgaagtt gctaaatcag gattaatgat atggtcaatt
     4441 cctaactctg atttaataaa ttctacctgc tttgtatatt caggatttct aatcctggca
     4501 atggttcttt tacaacctaa tctctttgcc aaagtacaaa taagagcatt ggtttcatca
     4561 ctttctgttg ttgccacgag taaatcatag gatctaatat tgatttcttt taaaacttct
     4621 atttctattc cattcgcaca aacggttagt acatctaaat aattattaat cccttctaag
     4681 actttttcat cctgatcgac tactgtaaca tcaacatctg tattagccaa tgtttctgcc
     4741 agtttatagc ccagcttccc tacacctaca ataattactt tcattgcacc tctgcctttc
     4801 tgatcttcat tcatatgata caacatcaat cgtaacaaaa atagctctat aaaacatgta
     4861 aaattatatc acttcttatc tgtcttttct acataaattg ttctggaagg aaaagcaatt
     4921 gatatctcta atttattcaa aatttccagt attttaagat ttacatcttc ttgtacttcc
     4981 aaatactttt gtctttctgt agctttcgta aagaaatgaa tgaaaatatc caatgagctt
     5041 tccccaaatc tttcgaagta tacatagatt gtttcaggat gtacttctgg atgttctttc
     5101 aacatttttc ttatttcttc cagacaagct ttaagctgtt ctaaaccagt attatagacc
     5161 aatcccaatc tgtaactgat caatcgtttg cccattttag accaattacg gataacttcc
     5221 ttactaagca ccgaattagg gatagtaaca agagtctgtt gaacagttct tattctggta
     5281 ctacgaaagc ctatttcttc tacagttcct tcaacactag gcgtctgaat ccaatcacct
     5341 atttcaaaag gcctgtcact cataatggct ataccaccaa ataagtttgc tgctgtttct
     5401 tgtgctgcaa gagcaaaagc cagtccacca agacctaaac ctgctaataa gccccctaat
     5461 tctttaaacc atacctgcaa tattgtaata cttcctataa tgacaataac gattttgata
     5521 ccattcttca aaaaagaaag caccataccg tctaattttt tatctgtttt ttctgtgatg
     5581 acctcatata atgaatcaaa aacatttgca atcctatagg ctgcccaaaa tatagtaaaa
     5641 agcacaatgg ttttggctat attattgatg actaatccta tatttacagg aaagctaatc
     5701 agttttaaag caatataaaa tgcaaaagtc acaaaagcta attccagtgg tttatcaagc
     5761 accataaata acttattcat ccaattacct tttgagtttt tagttagtct gttcaataat
     5821 tttataagca gttttataat ggctctcttt aaaacaattg tcaataaaat aatcaataaa
     5881 gccaaaagga tgttccacat taatatgtct tcaattttta tatcaaaatt tcccagagat
     5941 ttcatcttat cctcctattt tacataatgt tattagtata ataaaacaat ctagataatt
     6001 aatcaactcc taaatatcct ccttggtatt tcagtatcta tattaattgc taaaaatcga
     6061 caaaatattt aaaaactatt caaaaaatct acatttaatg atataataaa gaaaaaattt
     6121 aaatcaatac ggggggatag ctatgaacat tactgtatta aggaatcata ttcataaagt
     6181 caataaagca atgagttatt ttctaatttt tttagctgtg actgctcttg tttcttcttt
     6241 tgttctccgt atgcccatct tactggcaaa ctttgcaata tttctctttt catcaatatt
     6301 tatgatttat tcataccgta aacaaaaatt agaaatggta tcttcctata tattatcttt
     6361 ttccctcgtt ttattatttg tactgccttt gccaagtaat gaatacttgt ttatagccat
     6421 tattcctgtt atcgttgctt ctatgtactt taatcagaaa ttagtcatta taaccagtac
     6481 tgttttaaat atttccatcc ttgctaaaga acttttagca ggcaacataa acagttattt
     6541 tattacaaca cttcttatta ttgattttac tatccttatt ttactcactt tatccctgat
     6601 aggaaaccgc ttaatccatc ttgcaaatgc agaaataatt aaatcgaaca atttattatc
     6661 tcagttaaat gaaacaatgg aaatcattca aaaaacttca gaaaacctaa gtaaagatat
     6721 tcaaaattct aatttaaacc tggaattgat aaaaagaaat ggggatacag tgacccacag
     6781 tgtacaggaa atatcaaaag gaattattga gcaaacagaa agcgtttcaa ggattagcga
     6841 aatgatgaat gatgcagata aaagagtgga agaagttaac gaactttcta agcaattagc
     6901 tgatatctct acagaaacca gtgaaactgt acgtgaaggt tctgaaaaaa taaacaatat
     6961 ggcacatcaa atggacatta ttaaccaggc tgtaacaaaa tcttatgcaa cggtattaga
     7021 actgaatgag aatatggacg cagtcaacag ttttttaaca agtatagcgc aaatatctga
     7081 acaaacgaat ttattggctt taaatgccgc tatagaagct gccagagctg gtgaatcagg
     7141 aaaaggcttt gcagttgttg ctgaagaagt cagaaagctt gctgaacaaa gtgccacaac
     7201 caccgaacaa atcagtgaaa tcatcattaa tatccaggaa aaatccagag acgtcctgga
     7261 agaagttcaa aatggaaccc ttgctacgaa tgaaggagag aaaatagcaa aagccgttaa
     7321 tgaaaacttt gaaaaaatta atcagtcctt taagaagatt gatgaatata tctatgaaga
     7381 attaacgaag aaccaaaaca tttcatccct cttttcgcaa atacgggctg aaactgaaag
     7441 tattgcaacc atttctgaag aacactctgc tgctacagaa gaacttatgg caaccaccga
     7501 ggaacacaat gcaagcattg aaaccatcta caatgctatg ctggatattc aaaaagcctg
     7561 tgaaaatctg caaaacatcg ttaataataa ccgttaactt tcaaactcac atatatcccc
     7621 ctcaataaaa aagaaaggta ataaaatacc tttctttttt ttattcaata taagttttaa
     7681 tgactttagc tggtccgaca ataaaataat cacctatact tgcaggaata aaaaaagaat
     7741 ctcctgcttt aaaagaaata taaccgtttt tatattctaa gcttccttca ccttctataa
     7801 atatgagcaa attaaatctt tccccattta agctttcctt gctctcagta tgaatttcaa
     7861 gaagttctgt tgaaaaatat tcacatgaaa taagataatt tctagtattt ttgccttctt
     7921 tcacaagctt tccaacaact ttttccttac cagcaagatt tgaaaaagaa ataacatcta
     7981 aagccttctt aatatgcagt tctcttgaat ttccattttc atccgtcctg ttccagtcat
     8041 aaaccctata ggttgtatct gaattttgct gtatctctgc aattaaaatg ccttcaccaa
     8101 ttgcatgtac tgttcctgca gggataaaaa acacatcacc tttttttaca tctacgtaat
     8161 tcagaacatc ttccaggcta ccttcttcaa ttgctatttc aaaatctttt ttgctaatac
     8221 ctgctttaag accataaatc aattttgcat caggcttagc atccagaaca atccacattt
     8281 ccgtcttacc cagatcacct tcatgtatct tcgcatattc atcctgaggg tgaacctgaa
     8341 cggatagttt atcggatgca tcaattatct taaccaataa agggaactta ccatattttc
     8401 ttttccaaat ttctgtacct aatgcttcct ctccataaat atcaagcact tcttttaaac
     8461 ttttcccttc taattcacca tttgcaacca gactcatccc attttgatga caagctatct
     8521 cccagctttc accagtatga caggaaggaa ttgctttacc aaaaactttt ctaagttttt
     8581 cacctcccca tatagcttct ttatatatag gattcatctt taaaggatat agcataacaa
     8641 cctcctgtaa ttttcaactt atcttttgag catccttttt tacttaacta gacagctaat
     8701 atcattttta aaattaacaa cagcaataaa tgcaggggat aaaataaata ataaaaatat
     8761 ttgtttaatt taactttaac atttgtatga aaaaaaatga aaggaatgca aaaaacagca
     8821 aaaccttgt
//
LOCUS       DAOSUD010000080         8712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4254_length_8712_cov_21.634400, whole genome shotgun sequence.
ACCESSION   DAOSUD010000080 DAOSUD010000000
VERSION     DAOSUD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_8712_cov_21.634400"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLG49_09075"
     CDS             complement(<1..455)
                     /locus_tag="PLG49_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17500.1"
                     /translation="MNNDIDIIHVKKTKTIKITLLYIILSNIFFGLISYPYFIIFLLV
                     YMSNIIENLLTANEYDFFAELFNFPVLSFILFDLFLASLAIFILYSVIQKKFEDNFDF
                     IWKKINKNYSFFTVLRLILMLIEIALFFWCAKPYDNWIINNYFKDSSGDL"
     gene            complement(629..3250)
                     /locus_tag="PLG49_09080"
     CDS             complement(629..3250)
                     /locus_tag="PLG49_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOQ17501.1"
                     /translation="MKQKIIHSILLLALMVIAFLGMTNFNNIKVQIPVTEQNTDIAEE
                     KFEKKETPSKKKLKQRSQEGPFNYGEALQKAILFYEFQRSGKLDPSTLRNNWRGDSGL
                     EDGADVGLDLTGGWYDAGDHVKFNLPMAYTVSMLGWAIYEYEEAFEETGQLDYLLNEI
                     RWASDYLMKCHPSPDEFYYQVGNGHQDHAWWGPAEVLPMQRPAYKVDKNNPGSTVAGE
                     AAAALAVTSIVFKDTDLNYSKLCLKHAKELFEFADSTKSDAGYTASNGFYDSWSGFYD
                     ELSWAATWLYLATGDKTYLNKAESYVAYWGREGQTDKIAYKWAHCWDDVHYGAALLLA
                     RITDKPLYKECMEMHLDYWSVGYNGERITYSPGGMAYLDQWGNLRYVTTTAFLAFVYS
                     DWEGCSKEKVKTYQEFAESQINYALGDNPRNGSYMVGFGKNPPQAPHHRTAHGSWADS
                     QTIPNKHRHILYGALVGGPDQNDNYNDNIGDYVANEVACDYNAGLVGALAKMYLLYGG
                     EADPDFKAIETPEDEIFVEAAVNASGTNFIEIKAILNNQSGWPARVIENASFRYYMDL
                     SELISAGKRAEDIKTNINYHEGASISQLKHLKDNIYYVEIDFSGTKIYPGGQSSYKKE
                     VQFRLTAPEGSQWNSNNDFSFNGLTTGSPVKTEYIPVYEKGKLIYGKEPFSSVNMTSS
                     NSEEKSSEQENQNNKKFFDESKEKEKNILKENDNKDQSIARGVLKVQAFNTIRDANSN
                     TLYPKIKLINSGKEAVDLSKVTIRYYFTKDDEKQLSFFCDWSSAGPSNVKGTFISMKS
                     QKNNTDTYLEIGFNQGAGILNPGEELEIQIRIAKSDWSNFNQSNDYSFNSAASDYVDW
                     DKITVYLSNQLKWGIEP"
     gene            complement(3289..6507)
                     /gene="carB"
                     /locus_tag="PLG49_09085"
     CDS             complement(3289..6507)
                     /gene="carB"
                     /locus_tag="PLG49_09085"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOQ17502.1"
                     /translation="MPKRNDIKKILIIGSGPIIIGQACEFDYSGTQACKALKNLGYEI
                     VLVNSNPATIMTDPGLADYTYIEPLNKKNLTEIIAKERPDALLPNLGGQTALNLCSEL
                     YKDGVLDEYGVEVIGIKIDAIERGEDRIIFKEIMNKLNIEMPRSQAAYSVEEAEKIAK
                     ELGYPVVIRPAYTMGGTGGGLVYNIEELRVVANRGIAASMIGQILVEESVLGWEELEL
                     EVVRDSKNQKITVCGIENVDPVGVHTGDSFCVAPMLTVPKEVQGRLQEYAYRIIEHIG
                     IIGGCNVQFAYDPKSDRIVIIEINPRTSRSSALASKATGFPIAYISAMLAVGVTLDEI
                     PYWRDGSLDKYTPAEDFVVVKFPRWNFEKFHGAEDKLGTQMRAVGEVMSIGKNYKEAM
                     QKAVRSLEINRFGWGFAKDFHDQALEDLMIMLNEASSERHFIIYEALRKGATVDEIYE
                     ITHINKYFIDQMKELVLLEEEILKYQGKKLPDDLLIKAKKDGFSDAYLAKLLNATEPE
                     IRKQRLDLGIIQAFEAVPVSGVENAAYYFSTYNAKDNIPVTSQKKVMILGGGPNRIGQ
                     GIEFDYCCVHAVLALKDLGYETIMINCNPETVSTDYDTSDKLYFEPLTVEDVLAIYNK
                     EKPLGVICQFGGQTPLNIAKELENAGVRILGTSQKTIDLAEDRDRFREMMKKLDIPMP
                     ESGMAVTVEEALEIANRIGYPLMVRPSYVLGGRGMEIVHDEKMLKKYMAAAVDVTPER
                     PILVDRFLKHAIEAESDALSDGKNVFVPTVMEHIEYAGIHSGDSACVIPPVSIEKKHI
                     ETIIEYTKRIAKEMNVVGLMNMQYAIADDKVYVLEANPRASRTVPLVSKVCNIPMARI
                     ATEIIMADYNNDNYPIENFENKKIPHYGVKEVVLPFNMFPEVDPVLGPEMRSTGEVLG
                     LSDSFELAFYKAQEATQTVLPTQGTVLISVNDEDKNDILAAAKEFFNMGFKIVATEGT
                     CKFLREHGIDCQFIKKIYQGSPNIADSIKNKEIQLIINTPMTKRSEFDDSYIRKIAIR
                     YKVPYITTTTAALASAKGIAAYRNDQPKHKVKSLQEYHKDID"
     gene            complement(6653..7276)
                     /locus_tag="PLG49_09090"
     CDS             complement(6653..7276)
                     /locus_tag="PLG49_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOQ17503.1"
                     /translation="MGRKKDFNYFEAFVDLSKYSLEAAKLLNDTLNNFNTMLLLDKIK
                     EMHEIEHSADLAKHKVINRLFKEFLPPIEREDIISLSQKIDDITDAVEDVLIRIDIYN
                     VKSIPPEILEFTNLIVECCNSIHKALLEFQNFKKSKELPSLIIKVNSLEEEGDALYVN
                     AVRKMYRSSKDPITLMIWTEVLQRLEICCDACEDVADDIESVVMKNS"
     gene            complement(7297..8349)
                     /locus_tag="PLG49_09095"
     CDS             complement(7297..8349)
                     /locus_tag="PLG49_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOQ17504.1"
                     /translation="MTISLLDFLNQLQNNPTLLIIVILTLGVILVNGWTDAPNAIATC
                     VSTRSMTPKKAIIMAAVFNFLGVFIMTMINSTVAQTIYKMVDFRGDSKQSLVALCAAL
                     FAIVLWATAAWVFGIPTSESHALIAGLSGAAIALQKGFSGINGQEWIKVIYGLFLSTI
                     LGFFAGFIVVRITEFICKGIDRRKTLPFFQKAQVLSAAAMGFMHGAQDGQKFMGVFML
                     GIFLAMGQTDVTTFTVPVWLMVLCSVVMALGTSIGGYRIIKTVGMGMVKLEKYQGFSA
                     DSAAAICLLISSVFGIPVSTTHTKTTAIMGVGASKRISSVNWSIVKEMVLAWILTFPG
                     CGAVGYLMAYIFMKIF"
     gene            complement(8483..8656)
                     /locus_tag="PLG49_09100"
                     /pseudo
     CDS             complement(8483..8656)
                     /locus_tag="PLG49_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619674.1"
                     /note="involved in the sodium dependent uptake of proline;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
BASE COUNT         2750 a         1763 c         1314 g         2885 t
ORIGIN      
        1 agatcaccag aactatcttt aaaataatta ttaattatcc aattgtcata gggctttgca
       61 caccaaaaaa acaatgctat ttctataagc attaaaatta acctcaaaac tgtaaaaaaa
      121 gaataatttt tattaatttt tttccatata aaatcaaaat tgtcttcaaa ttttttctgg
      181 ataacgctat ataaaataaa aatagcaaga ctggcaagaa ataagtcaaa taagataaaa
      241 cttaaaacag gaaaattaaa taattctgca aaaaaatcat attcatttgc agtgagaaga
      301 ttttcaataa tattagacat gtatactagt aaaaaaatta taaaataagg ataactaatt
      361 aatccaaaaa agatattgga taaaataata tataacaaag ttattttaat agtttttgtt
      421 tttttgacat gaataatatc aatatcatta ttcatatttt cctcctaata tttatatgca
      481 taattgctta taggattata atattatatt tttcaacaat tataaattat tttaaaatat
      541 tttataaaat attaaactct gtaaataaat atgtattaca aaaagtgacc gtatactaat
      601 agtaagatta gtatacggtc acttttcatc agggttctat tccccatttt aattgattag
      661 acaaataaac cgttatctta tcccaatcca cataatcact ggcagcagaa ttaaatgaat
      721 aatcgttact ttgattaaag ttgctccagt cagattttgc aattctgatt tgaatctcca
      781 gttcttctcc aggattaagg attcctgctc cctgattaaa gcctatttcc aagtaagtat
      841 ctgtattatt tttttgactc ttcatggaaa taaatgtccc tttaacattt gaaggaccag
      901 ctgatgacca atcacaaaag aaagacagtt gtttttcatc atcctttgta aaataataac
      961 ggatggttac tttagataaa tcgacagctt cttttccact atttattaat ttaatctttg
     1021 gatacaaggt attggaatta gcatctctga tggtattaaa ggcctggact tttaatacgc
     1081 ccctggcgat gctttgatcc ttattgtcat tttctttaag gatatttttt tctttctctt
     1141 tactttcatc aaagaacttt ttattatttt gattttcctg ttcagaagat ttttcttctg
     1201 aattggatga agtcatattg acacttgaaa aaggttcttt tccatagatt aattttccct
     1261 tttcatatac agggatatat tccgttttga ctggcgatcc ggtcgtcagt ccattaaaag
     1321 aaaaatcatt atttgaattc cactgactgc cttccggggc agtaagcctg aattgaactt
     1381 cttttttata tgaggattga ccaccaggat aaatttttgt tcctgaaaaa tcaatttcta
     1441 cgtaatagat attatctttt aaatgtttta actgagaaat ggaagcccct tcatgataat
     1501 tgatatttgt cttaatatct tctgcacgct ttccagcact gataagttca gacaaatcca
     1561 tataatacct gaaagaggca ttctctataa ctctagccgg ccatccagac tgattattta
     1621 aaatagcttt tatttctata aaattagtac ctgaagcatt aacagctgct tccacaaaaa
     1681 tctcatcttc aggggtttcg attgctttaa agtcaggatc ggcttctcct ccataaagta
     1741 ggtacatctt ggctaaggca cctactaaac cagcattata gtcacatgcc acttcattgg
     1801 ctacataatc tcctatatta tcattataat tgtcattttg gtctggtcca cccactaagg
     1861 caccatacag gatatggcgg tgtttattgg gaatggtctg actgtcagcc caggaaccat
     1921 gtgccgtacg atgatgcggg gcctgaggag gatttttgcc aaaaccaacc atatagctgc
     1981 catttctcgg attgtctcct aaggcataat taatctgact ctcagcaaat tcctggtatg
     2041 tcttaacctt ttctttactg cagccttccc agtctgaata gacaaaggcc aaaaaggcag
     2101 tagtcgtaac ataacgtaaa ttaccccatt gatccagata agccatgcca ccaggactat
     2161 atgtaattct ttctccatta tatcccactg accagtaatc cagatgcatt tccatacatt
     2221 ctttatacaa tggcttgtct gtaatccggg ccagcaacaa agctgcgcca tagtggacat
     2281 catcccagca atgagcccat ttataggcaa tcttatccgt ttgaccttct cttccccaat
     2341 aagctacata agattctgcc ttatttagat aagttttgtc tcctgtagcc aggtaaagcc
     2401 aggttgcggc ccatgacaat tcatcataga aaccactcca ggaatcataa aaaccatttg
     2461 aggccgtata acctgcatca ctttttgtac tgtcagcaaa ctcaaataat tctttggcgt
     2521 gttttaaaca taactttgaa taattcaagt ctgtatcttt aaaaacaata gatgttaccg
     2581 ctaaagccgc agcagcttca cctgctacgg ttgaacccgg gttatttttg tcaactttat
     2641 aagcaggcct ttgcatgggg agtacttctg caggacccca ccaggcatga tcctggtgcc
     2701 cgttaccaac ttgataataa aactcatctg gtgagggatg acatttcatt aaataatctg
     2761 aagcccatcg gatttcattt aataaatagt ctaattgtcc agtttcctca aaagcttctt
     2821 catattcata aattgcccat cccagcatgc tcaccgtata tgccattggt aaattgaatt
     2881 taacatggtc accagcatca taccaaccgc cggtaagatc cagaccaacg tctgccccgt
     2941 cttccagtcc ggaatctcca cgccagttat ttctaagagt agatggatcc aatttcccag
     3001 aacgctgaaa ttcataaaac aatatggcct tttgtaaggc ttccccgtaa ttaaaaggac
     3061 cttcctgaga cctctgtttt agttttttct ttgagggtgt ttcttttttc tcaaattttt
     3121 cttcagctat atctgtattt tgctctgtca caggtatttg aacttttatg ttattaaaat
     3181 tagtcattcc caaaaaagca ataaccatta aagcaagaag caaaatggaa tgaattatct
     3241 tttgtttcat ctaccttccc ccagaaagat aaaggtattt tacttatttt aatcaatatc
     3301 tttatgatat tcctgtaaag attttacttt gtgtttaggc tgatcatttc tgtaagcagc
     3361 tatacctttt gcactggcta aggctgccgt tgtcgttgta atatagggta ccttatagcg
     3421 gatagcaatt ttgcggatat atgaatcatc aaattcactg cgcttggtca tcggtgtatt
     3481 aataatcagt tgaatttcct tatttttaat actatcggct atattgggcg aaccctggta
     3541 gatttttttg ataaactgac aatcaatgcc gtgttctctt aaaaatttac aagtaccttc
     3601 tgttgcaaca attttaaagc ccatattgaa aaattcttta gcagcggcta aaatgtcatt
     3661 tttgtcttca tcatttacac taataagtac tgtaccttgt gtaggcaaga cagtttgtgt
     3721 ggcctcttgt gctttataga aagccagttc gaaggaatcc gaaagcccta atacttctcc
     3781 tgttgaacgc atttcaggtc ctaatacagg gtcaacttca gggaacatat taaagggtaa
     3841 aaccacctct ttaacaccat aatgaggtat tttcttattc tcaaaatttt ctattggata
     3901 attgtcatta ttataatctg ccataattat ttccgttgca attcgagcca taggaatatt
     3961 acagacttta gaaactaaag gtactgtcct ggaagctctt ggattagctt ctaagacata
     4021 aactttatca tcggctatgg catattgcat attcataaga cctacaacat tcatttcctt
     4081 tgcaattctt ttagtatatt ctataatcgt ttcaatgtgt ttcttttcaa tacttaccgg
     4141 tgggatcaca caagcagaat ctccggaatg gatacctgca tattcaatat gctccataac
     4201 tgttggtaca aatacatttt taccatccga taaagcatcg gattcagctt ctattgcatg
     4261 ctttaagaac ctgtccacta agattggtct ttcaggtgta acatctactg ctgccgccat
     4321 atattttttc aacatttttt cgtcatgcac aatttccata ccccgaccac caagaacata
     4381 tgaaggtcga accattaaag gatagccaat acggttggca atttctaagg cttcttcaac
     4441 tgttacagcc atacctgatt caggcatagg gatatccagc tttttcatca tttcgcggaa
     4501 tctatccctg tcttcggcca aatctatagt tttttgagaa gttcctaaaa ttcgcacacc
     4561 agcattttct aattctttgg caatatttaa aggtgtctgg cctccaaatt ggcaaataac
     4621 gcctaatggt ttttctttat tgtaaatggc taaaacgtct tcaacagtca atggttcaaa
     4681 atacagctta tccgaggtat cgtaatctgt tgatactgtt tcaggattac aattaatcat
     4741 gatggtttcg tatcctaaat ctttaagggc aagaacagca tgaacacaac aatagtcaaa
     4801 ttcaatccct tgaccaatcc tgtttggtcc tcctccaaga atcataactt ttttctgact
     4861 tgttaccggg atattatctt ttgcattata cgtagaaaaa taataagctg cattttcaac
     4921 accgctcaca ggcactgctt caaatgcctg tataatgcct aaatccagtc tttgcttacg
     4981 aatttcaggc tcagtagcat ttaataactt agctaaatat gcatctgaaa atccgtcttt
     5041 tttagctttg attaacaagt catcaggtaa tttctttccc tgatatttta agatctcttc
     5101 ttctaaaaga acgagttctt tcatctggtc aataaaatat ttattaatat gggtgatttc
     5161 atagatttca tcaactgttg cacccttgcg taaagcttca taaataataa aatgtctttc
     5221 actgctagct tcattaagca taatcattaa atcttcaagc gcttggtcat gaaaatcttt
     5281 tgcaaatccc cagccaaaac ggttaatttc aagggaacgt acagcctttt gcatggcttc
     5341 tttataattt ttaccaatgc tcatgacttc cccaacggcc cgcatttggg tacctaattt
     5401 atcttctgct ccatggaatt tttcaaaatt ccagcgtgga aatttaacca ccacaaaatc
     5461 ttctgcaggc gtatatttat ccagactgcc gtctctccaa taggggattt catctaaagt
     5521 cacacccaca gccagcatgg cagaaatata ggcaatagga aaaccagttg cttttgaagc
     5581 cagggctgac gaacgggaag tccttggatt aatttctata atcacaatgc ggtctgattt
     5641 aggatcataa gcaaattgaa cgttacaacc tccaataata ccaatatgtt caataatcct
     5701 ataggcatat tcttgcagtc ttccctgaac ttctttggga acagtcagca ttggagcaac
     5761 gcaaaaagag tcccctgtat gaaccccaac cgggtcaaca ttttcaatac cgcaaactgt
     5821 aatcttttga tttttactgt ctctgactac ttcgagttca agttcttccc agccaagaac
     5881 agattcttct acgaggattt gtccaatcat actggcagca ataccacggt tagcgacaac
     5941 tcttagttct tcaatattat aaaccaaacc tccaccagtt cctcccatgg tataagctgg
     6001 cctaattaca accggatatc ctaattcttt tgcaatcttt tcggcttctt ccacactata
     6061 agcagcctgg cttcttggca tttcaatatt aagcttattc ataatttctt taaatataat
     6121 gcggtcttct ccccgctcaa tcgcatctat tttaatacca ataacctcga caccatattc
     6181 atctaaaaca ccatctttat atagttctga acaaagatta agggctgttt gtccgcctaa
     6241 attaggtaaa agagcatctg gtctttcttt tgctataatt tctgttaaat tctttttatt
     6301 taaaggctca atatatgtat aatccgccaa cccaggatcc gtcataatgg ttgctggatt
     6361 agaattgacc agaacaattt catatccaag attttttaat gctttacatg cttgagtccc
     6421 tgaatagtca aattcacaag cttgtccaat aataataggc cctgaaccaa taatcagtat
     6481 ttttttaata tcatttcttt taggcatcgt attcctcctt aatgtccaat ctaaacttct
     6541 gatcaaaaag ttaaatggtt aaaatctatt ttattataac acaattgcaa attcattaaa
     6601 aataaaattc atttttctat aaaaaaaaac gtctcaatag agacgtcatt ttttatgaat
     6661 ttttcatgac aacactttcg atatcatctg ctacatcttc acacgcatca caacaaattt
     6721 ctaatctttg aaggacttct gtccaaatca tcagagtaat gggatctttg gaactcctgt
     6781 acatttttct tacagcattg acatacaaag catcgccttc ttcttctaaa ctattgactt
     6841 tgataattaa tgaaggcaac tcttttgatt ttttgaagtt ttgaaattct aataaagctt
     6901 tatgtattga attacagcat tctacaatta aatttgtaaa ttccaatatt tctggtggga
     6961 ttgattttac attatagata tcaatacgga ttaagacatc ctcaacggca tctgtgatat
     7021 cgtcaatttt ttgtgaaaga ctaatgatat cttctcgttc aataggcggc aaaaactctt
     7081 taaaaagtct gttaatcacc ttgtgttttg ccaggtcagc agaatgttca atctcatgca
     7141 tttctttaat tttgtccagt aaaagcatgg tattgaaatt atttaaggta tcatttaata
     7201 atttggcagc ttctaaagaa tacttggata aatccacaaa agcttcaaaa taattaaagt
     7261 ctttttttct tcccattgtt ctctctcctt atcctattaa aaaattttca taaagatata
     7321 tgccattaaa tatccaacgg ctccacagcc gggaaaagtt aatatccaag ccaataccat
     7381 ttcttttaca atactccagt tcactgaaga aatgcgtttg gaggcaccaa cacccataat
     7441 agcggttgtt ttcgtatggg ttgtacttac gggaatacca aaaaccgatg atatcaaaag
     7501 gcatatggct gcagcgctat cagctgagaa accttgatac ttttctaatt ttaccatacc
     7561 cattcctacc gtttttataa ttctgtagcc tccaatagag gttcctaaag ccatcacaac
     7621 agaacaaagc accattaacc agacaggtac agtaaaagtg gttacatctg tttgtcccat
     7681 ggcaaggaaa atgccaagca taaatacacc cataaatttt tgcccgtctt gtgccccatg
     7741 cataaagccc atagcagcag cacttaaaac ctgggctttc tggaagaaag gaagggtttt
     7801 ccgtctgtca attcctttac agataaactc tgtgatacga acaacaataa agcctgcaaa
     7861 aaaacctaaa atagtagaca aaaacaagcc ataaataact ttaatccatt cttgtccatt
     7921 aatccctgag aagccttttt gaagggctat ggctgcacct gacaagccag caattaatgc
     7981 atgactttca ctggtaggga taccaaaaac ccatgcagca gttgcccaca aaacaatggc
     8041 aaacagtgcg gcacaaagag caactaagga ttgtttagaa tcgcctctga aatcaaccat
     8101 tttataaatg gtttgtgcaa cggttgaatt aatcatggtc ataataaata cacctaaaaa
     8161 attaaaaact gcagccataa tgatcgcttt tttaggcgtc atcgaacggg ttgaaacgca
     8221 cgtcgcaatt gcattaggtg catcggtcca gccattaacc aggataacac ctaaagtaag
     8281 aataacaata atcagtaagg taggattatt ctgaagttga ttaagaaaat caagcaaact
     8341 aattgtcata gcttttcctc caatattata gtcttaaatg caaaccaaag atattataac
     8401 atttcagttt aagcataaca acctaataaa atttataaat taaaaaacct tttctggttc
     8461 tgtcagaaaa ggtttttaac tgttattgaa ctgttcttgc cagttcaaat tctttttcaa
     8521 tttcttctga aggttttttt gaaagtaacg atacaaccac aatggctata caagaaataa
     8581 caaatgccgg caacaattca taaatagcaa atacccctcc taatggactt aaaagcctat
     8641 cccaaacaaa aaccatgata ccgggttgac cccctataat tggagattat ctgcgtaata
     8701 atttttctac ct
//
LOCUS       DAOSUD010000081         8557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4324_length_8557_cov_23.457539, whole genome shotgun sequence.
ACCESSION   DAOSUD010000081 DAOSUD010000000
VERSION     DAOSUD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4324_length_8557_cov_23.457539"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1170
                     /locus_tag="PLG49_09105"
     CDS             34..1170
                     /locus_tag="PLG49_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ17505.1"
                     /translation="MDFLISGISSITPQQLVMYAVGILLIYLAICKDFEPALLLPMGF
                     GAILVNLPNSGVLNQTLAGIGETNGIIEWLFNIGIEASEALPILLFIGIGAMIDFGPL
                     LSNPKMFLFGAAAQFGIFFTISLACLLGFDLKDAASIGIIGAADGPTSILVSQILGSK
                     YIGPIAVAAYSYMALVPIVQPFAIRLVTTRNERLIKMPYNPKSVSKTTRILFPIVVTF
                     IAGLVAPMSVALVGFLMFGNLLRECGVLNSLSETAQNTLANLITLLLGITISFKMTAD
                     AFVSLDTLLIMVLGLIAFVFDTIGGVLFAKILNLFLKEKINPMIGAAGISAFPMSARV
                     IQKMGLKEDPTNHLLMHAVGANVSGQIGSVVAGGIILSLLTQIL"
     gene            1192..1326
                     /locus_tag="PLG49_09110"
     CDS             1192..1326
                     /locus_tag="PLG49_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17506.1"
                     /translation="MNIENFLISLGIMGKGMLGIFVVIIIIMIIVNLLTKTSSKNSEN
                     "
     gene            1400..1945
                     /locus_tag="PLG49_09115"
     CDS             1400..1945
                     /locus_tag="PLG49_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ17507.1"
                     /translation="MKELMELFMVFARIGAFTFGGGYAMLPLIEKEIVEKRKWASRDE
                     ILDYFAIGQCTPGIIAVNTATFIGYKRKGIIGSLFSTFGIVTPSFIIILIIASFIKHF
                     QDYAIVQHAFGGIRIAVAALIVHTIIKLWKQSIKDWSAIVLFIISFLAITFFNLSPVI
                     MIILGGIAGAYKNTRKKADEK"
     gene            1942..2505
                     /locus_tag="PLG49_09120"
     CDS             1942..2505
                     /locus_tag="PLG49_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005212625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ17508.1"
                     /translation="MIYLLIYYEFFKIGLFAIGGGLATIPFIQDLAQRYNWISIEELI
                     DMIAISESTPGPIGINIATYTGYKISGVFGSLAATLGIITPSIIIILLIAHFYMKFSE
                     EPIVRAGLTGIRPAVIGLIAVAAFEVIKIALFNIEKFYETKVLLDIFNFKAIVLFSLL
                     LFFIQKYKKHPILYIAASAVIGILIKY"
     gene            complement(2534..3586)
                     /locus_tag="PLG49_09125"
     CDS             complement(2534..3586)
                     /locus_tag="PLG49_09125"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOQ17509.1"
                     /translation="MSIVKTSFGKSKDGVEAFLFKLTNSKGMEVGITNYGAILVSIKV
                     PDKAGNFDDVLLGFDQLDDYLKDHPYFGAVVGRHANRIENAEFTLNGKTYHLAKNDGN
                     NHLHGGYEGFDKKFWEAEIININNRESIQLSYFSKDGEENYPGNLEVKVIYTLTEDNE
                     LIIDYYAVSDQDTVVNLTNHAYFNLKGHNKGDILDHQLMINADQFTVINNECIPTGEI
                     RNVKGTPMDFTSLRSIREGLLSDDEQIKCGKGYDHNWILNVSGKAAEKAGELYEPESG
                     RLMEFFTTKLGVQFYSGNFIDVVGKAGAKYIKHSGLCLETQYFPNSLKHKHFPSAVLK
                     AGDKYQHTTIYKFSTR"
     gene            complement(3625..4932)
                     /locus_tag="PLG49_09130"
     CDS             complement(3625..4932)
                     /locus_tag="PLG49_09130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOQ17510.1"
                     /translation="MKRKLSLMMLVILLFIHIIPVYAQEPSFLDSELQSDAVILIEEE
                     TGRVLFEKNSNKKIYPASLTKILTALIAIEYLDPNEVITVGNEVYAVPLDASKAGHNP
                     GDEITLKDLLISLLLPSGNDSAFVIACQVSKKATGNANLDVNGCIAHFAGMMNEKAKE
                     FGLSDTHFVNPHGYHDENHYSTAHDIAQLTREALKNPLFKEIVKMPSAEIGTAGNPKE
                     QKLTFKNRNLLLDSKNSETFYSYATGVKTGFTNEAGQCLVASAEKDGMSLIAVVLNNP
                     TSVGRFKEAKTLLEYGFQNYNFQNIVNEGDTITTVVVEKAAPKGPSELELVAKNDFKE
                     LFYQDDFSRVEKSVTLFNEPIEAPIVKGQVIGEVSFILDGEQLGAVELIAKYDIQKRT
                     ILDLIFSVNAIPYWCGAVGIIFILVSVITAIKKKRKRRGLHFR"
     gene            complement(5102..6277)
                     /locus_tag="PLG49_09135"
     CDS             complement(5102..6277)
                     /locus_tag="PLG49_09135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ17511.1"
                     /translation="MDNFVFYNPTKIIFGIDTENQVGEEVARYSKKILFVYGGGSIKR
                     TGLYDRIVASLRKSQVEFIELPGVKPNPRLSLVREGIKICRDNQLDFILAVGGGSTID
                     TAKAIAIGVPYDGDVWDFYSKNVAPQAALPVGTVLTIPAAGSESSDSSVITNEEGWYK
                     RGVGSELIYPKFSILNPKIASTIPKNHVAAGVADIMAHLMERYFTNTKNVELTDRLIE
                     STMKTVINHAPVVIRDPDNYEAWSQLMWSGTVAHNNLFSTGRVGDWASHNIEHELSGI
                     YDVAHGAGLAVVFPAWMKYVYKHDLNRFVQFAVRVWNVEEDFFNPEKTALEGIKKLEE
                     FFISIGLGVYLEDLGIQDDRLEEMADKATNSNQRKLGNFVKLDKNDVYSILKLAKRG"
     gene            complement(6309..8438)
                     /locus_tag="PLG49_09140"
     CDS             complement(6309..8438)
                     /locus_tag="PLG49_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ17512.1"
                     /translation="MADKKKGFKFLKKQDKVEKVRNNKVLKESTNKRKRKDIFLNPMK
                     NVGIAQKLIITFIILSIIPLSIIASFAYENAETTVENKVGYYSQKMVEQLAVNLDFKI
                     DEYEKIPRMIIGNDELMKSLLREKFATINEEKQNEQSIQKLLFSMNFSNAGMEALYIY
                     KTSGALYRAGSRLNLTSELKGTSELDIFKDLAKIKKESKESFLWITGYNNTYKYIILV
                     KELSTTDDVGIIVVLINSEELGSIVDAAEINGETSIMVLDTNRKVISHQDSSLLGSEL
                     TDGYLDNIYGGKESDHFRDKDDVISFATTKTGWKLITKQPMSVLMEEMQIVRQGIIVV
                     VFICIILSIIIGMYMAFSISKPLNVIIRLMGKVEQGDLTVSSPITGKNEIGKLSYSFN
                     KMIENVRELIIKTDSVSQEVVKGANIIKSSSEQSAAAASQVASAITELAEGASEQTRQ
                     VENTNQLMDRLANNINNVIERIEEIMKMIEKAEASRDYASSTMDRLNEKTKNVVESSN
                     IINQEIQLLNEETKEVIKVVKVIAGISEQTNLLALNAAIEAARAGKAGKGFAVVADEI
                     RKLAMETKDATGMINKIISNIQTKTQKAVEIVESSDKIFDEQKLMVLEMNDAFIEMAE
                     STQKMIEQIEEINSKIADISQQKDQTVSAISNIAAIVQQSAASIEEVTATSQEQTTAA
                     EQLSILANNLNEVIESLKKSLAQFIIN"
BASE COUNT         2569 a         1649 c         1273 g         3066 t
ORIGIN      
        1 attttttcta taaaataagg aggaaatatt aacatggatt ttttaatatc aggtattagt
       61 tcaattacac ctcaacagtt agtgatgtac gccgttggta tattacttat ttatctggcc
      121 atatgtaaag actttgagcc agcattattg cttccaatgg gttttggtgc tatcctggtt
      181 aatttaccaa actccggggt tcttaatcaa acccttgctg gtattggaga aacaaatgga
      241 attatcgaat ggttatttaa tataggtatt gaagcttcag aggctttgcc tattttactc
      301 tttataggta tcggggcaat gatagatttt ggtcccctgc tttcaaatcc aaagatgttt
      361 ttatttggtg cagcagctca atttggtata ttttttacca tatcccttgc ctgcttatta
      421 ggcttcgatt taaaagatgc tgcttctatt ggaattatcg gtgcagcaga tggtcctacc
      481 tctattcttg tttcacaaat tttaggttca aaatacatag gtcctattgc cgtggccgcc
      541 tattcttata tggctttggt acctatcgtg cagccctttg ctatccgatt ggttactaca
      601 agaaatgaac gtttaataaa aatgccctat aatcctaaat ctgtttccaa aacaaccaga
      661 attctcttcc ctattgtggt tacttttatt gcaggccttg tagctcctat gtctgtggcc
      721 ttggtaggat tccttatgtt tggaaattta ctcagagaat gcggtgttct taacagcttg
      781 tctgaaacag cccaaaatac tttggccaat ttaatcactt tactgcttgg catcaccata
      841 tcttttaaga tgactgctga cgccttcgta tcactagata ccctccttat tatggtttta
      901 ggtctgattg cttttgtatt tgataccatt ggcggtgtat tatttgcaaa aatccttaac
      961 cttttcttaa aagaaaaaat taatcctatg attggggcag caggtatatc tgcctttccg
     1021 atgtcagcaa gagttattca aaaaatgggc ttaaaagaag atcccaccaa tcatctttta
     1081 atgcatgcag ttggtgctaa tgtatcagga caaatcggct cagtcgttgc aggaggtatt
     1141 atattaagcc tgctaacgca aatattatag gtttgtaagg aggattgaat catgaatata
     1201 gaaaacttct taatctcttt aggaatcatg ggaaaaggta tgctgggcat ttttgtggtc
     1261 atcataatca ttatgattat tgtgaacttg ttaacaaaaa catcgtctaa aaatagtgaa
     1321 aattaagcta ttggtttcat cccaatagct ttttttaact tttttatata cttaagacta
     1381 gacagaaagg agctagacag tgaaggaact tatggaatta tttatggtat ttgcccggat
     1441 aggtgcattt acctttggtg gaggctatgc catgcttccc ttaattgaaa aggaaattgt
     1501 agaaaaaaga aaatgggcaa gcagggatga aattttagac tattttgcta ttggtcagtg
     1561 tacacctgga attatagcag tcaatacagc cacctttatc ggttataaaa gaaaaggaat
     1621 tattggttcc ctcttttcaa cctttggaat cgtaacccct tcttttatta tcattttaat
     1681 tatcgccagc tttataaaac actttcagga ttatgccatc gtccagcatg cctttggagg
     1741 tattagaatt gcagtagcag cccttattgt ccatacaatt attaaattgt ggaagcaatc
     1801 cataaaagac tggtctgcaa tcgtattatt cattatttct tttcttgcta ttacattttt
     1861 taatctttct ccggttatta tgattattct aggaggcatt gcaggcgcat ataaaaatac
     1921 acgaaaaaag gcggatgaaa aatgatttat ctcttaattt attacgaatt ttttaaaatt
     1981 ggtttatttg ccataggcgg tggtctggcc actattccat ttatacaaga tttggctcag
     2041 cgttataatt ggattagcat agaagaatta atcgatatga ttgccatatc tgaatcaaca
     2101 cctggaccaa taggtattaa catagcaact tacacaggct ataaaatttc tggcgttttt
     2161 ggttccctgg ctgctacctt aggaatcata accccttcaa ttattattat tttgttaatt
     2221 gcccatttct atatgaaatt tagtgaagaa cctattgtcc gggctggtct tacaggaatc
     2281 cggcccgcag ttattggtct tattgctgta gcggcttttg aagtgataaa aattgcttta
     2341 tttaatatag aaaaattcta cgaaacaaaa gtccttttgg atatcttcaa ttttaaagct
     2401 attgttcttt ttagtttatt attatttttt atacaaaaat acaaaaaaca ccctatatta
     2461 tatatagctg cttctgcagt tatcggaatc ctgattaaat attaaaaaag gatgcctcag
     2521 gcatcctttt tcattatctg gtagaaaatt tataaatggt tgtatgctga tatttatctc
     2581 ctgcttttag aactgctgaa ggaaaatgtt tatgctttaa agaattgggg aagtactgag
     2641 tttcaagaca aagcccactg tgtttaatat attttgcacc tgcttttcca acgacatcaa
     2701 taaagtttcc agaataaaat tgtacaccca gttttgttgt aaagaattcc attaatcttc
     2761 cactttcagg ctcatagagt tcccctgctt tttcagcagc ttttccactg acatttaaaa
     2821 tccagttatg atcgtatcct tttccacatt taatttgctc atcatcggac aacaaacctt
     2881 ccctaatgct tcttaagctt gtaaaatcca taggcgtacc ttttacattc ctgatttcac
     2941 ccgttggaat acattcgtta ttaataactg taaattgatc tgcattaatc attaactgat
     3001 gatcaagaat atctccctta ttatgacctt taaggttaaa gtaagcatgg ttcgtaagat
     3061 taaccactgt atcctggtca gataccgcat aataatcaat aattaattca ttgtcttctg
     3121 ttaaagtata aatcacttta acttcaagat tacctgggta attttcttct ccgtccttac
     3181 taaaatagct aagttgtata gattctctat tatttatatt aatgatttct gcttcccaaa
     3241 acttcttatc aaatccttca tacccgccat ggagatgatt gttaccatca ttcttagcaa
     3301 gatgataagt ctttccattt agagtaaact ccgcattttc aattctattg gcatgtctgc
     3361 ctaccactgc tccaaaataa ggatggtctt ttaaataatc atcgagttga tcaaaaccaa
     3421 gaagcacatc gtcaaaatta cctgctttat ctggcacttt tatggaaaca agaattgcgc
     3481 cgtaattggt aattcctact tccatccctt tagaatttgt taatttaaaa agaaacgctt
     3541 caactccatc tttgcttttt ccaaagcttg ttttaacaat actcataaaa ttcctcctta
     3601 gtttatataa tgtagaattt atgactatct aaaatgaaga cctcttcttt ttcttttctt
     3661 ttttatagca gtaataacgg ataccaatat aaaaataatt ccaaccgcac cacaccaata
     3721 aggaatggca tttacagaaa aaataaggtc taagatggtg cgtttttgta tatcatattt
     3781 tgcaatcagt tctacagcac ctaattgttc cccatcaagt ataaaactaa cttctccaat
     3841 tacttgtcct ttaacaatag gcgcttctat aggctcatta aacaaagtca cggatttttc
     3901 tacccgcgaa aaatcatcct ggtaaaagag ttccttaaaa tcatttttag caactaattc
     3961 tagttcagaa ggtcctttgg gagctgcttt ttctactact actgtcgtta tggtgtcacc
     4021 ttcattgacg atattttgaa aattataatt ctgaaaacca tattccaata aagttttggc
     4081 ttctttaaaa cgacccacag aagttggatt attaagtact actgcaatca aactcatgcc
     4141 atctttttca gctgaagcca caaggcattg acctgcttca ttggtgaaac cagtttttac
     4201 acctgtcgca taagagtaaa aggtttcact atttttcgaa tctaaaagta aatttctatt
     4261 tttaaaagtt aatttttgct ctttcggatt tcctgcagtt ccaatttcag ccgatggcat
     4321 ttttacaatt tccttgaata agggattttt taaagcttcc ctggtcagtt gggctatatc
     4381 atgagccgtc gaatagtgat tttcatcatg ataaccgtga ggatttacaa aatgagtatc
     4441 tgaaagtcca aattccttgg ccttttcatt catcataccg gcaaaatgag ctatgcaacc
     4501 attgacatca agatttgcat taccagtggc tttcttagaa acctggcagg caatcacaaa
     4561 agcagaatca ttacccgaag gcagcagcaa agaaattagt aagtctttta aagtaatctc
     4621 atcgccagga ttatgtccag ccttgctggc atctaaaggt acggcatata cttcattccc
     4681 aaccgttatg acttcatttg gatctaagta ttctatggca attaatgcag ttaatatttt
     4741 tgtcagactg gctggataga tttttttatt tgagtttttc tcaaaaagta cccttccggt
     4801 ttcttcttct attaaaataa ctgcatctga ctgcaattcc gaatccagaa atgaaggctc
     4861 ctgagcataa actggaatta tatgaataaa caagagaatg accagcatca ttagagataa
     4921 ttttcttttc acctttaagc tcctttcaat acaagtataa ttatccgtac ccattgtaac
     4981 ataaaaatat aaaaaaaaca gcctaataaa acaaatcttt attgtggagc atatactttt
     5041 tttacatata taaaagccca gggaagaatc cccaggcttt tattaatgct ttaaacacag
     5101 tttatcctct tttagccagt tttaaaatac tataaacgtc attcttatcc aatttaacaa
     5161 aatttcctaa tttacgttga ttagaattgg ttgctttatc ggccatttcc tctagacggt
     5221 catcttgtat acctaaatct tctaaataca caccaagccc tatagaaata aagaattctt
     5281 caagcttttt gattccttct aaagctgtct tttcaggatt aaagaaatct tcttcaacat
     5341 tccataccct tacggcaaat tgaacaaagc ggtttaaatc atgcttatat acatatttca
     5401 tccaggcagg gaaaaccact gctaaacctg caccatgggc aacatcataa atgccgctta
     5461 attcatgctc aatattatga gaagcccaat cacccaccct gccagtacta aaaagattgt
     5521 tatgagcaac agtacctgac cacattaact gactccaggc ttcataatta tcaggatcac
     5581 gaatgaccac cggagcatga ttaataacgg ttttcatagt ggattcaatt aatctgtcgg
     5641 ttaattcaac atttttagta ttcgtaaaat atctttccat aagatgagcc attatatccg
     5701 ctactccggc agcaacatga ttcttcggaa tggttgaagc aattttaggg ttaagaatag
     5761 aaaacttagg atagatcagt tctgacccta cccctctttt ataccaacct tcttcattgg
     5821 ttattacaga actgtcactg gattcactgc ctgcagcagg tatggtcaaa actgtaccaa
     5881 ctggtaaagc tgcctgagga gcaacatttt tactgtaaaa atcccataca tctccgtcat
     5941 aaggtactcc tattgcaata gcttttgcag tatcaattgt acttcctcca ccaacagcaa
     6001 ggataaagtc cagttgatta tctctgcaaa tctttattcc ttcccttacc aggcttaatc
     6061 tcggattggg ttttacgcca ggaagttcta taaattcaac ctgcgacttt cttaaggaag
     6121 ctacaatcct gtcataaaga cctgttcttt ttatactgcc tccgccataa acaaacaata
     6181 tttttttact atacctggcc acttcttctc ctacttgatt ttcagtatcg ataccaaaaa
     6241 taatcttcgt aggattgtaa aacacaaaat tatccatggt cattcctcca atgctgataa
     6301 taatatgatt aattaataat aaattgtgct aaagatttct tcaagctttc aatgacttca
     6361 tttaagttat tggcaagaat tgacagttgt tctgctgcag ttgtttgttc ctggctggtt
     6421 gccgtaacct cttcaattga agcagcactt tgctgtacaa tcgctgcaat attactaatt
     6481 gcactaaccg tttgatcttt ttgttgagaa atgtctgcaa ttttactatt aatctcttca
     6541 atttgctcaa tcattttttg agttgattct gccatttcaa taaaagcgtc attcatttca
     6601 agtaccatta atttttgttc atcaaaaatc ttatctgaac tttcaacaat ttctactgct
     6661 ttttgagtct ttgtctggat attggaaata attttgttaa tcatacctgt tgcatctttt
     6721 gtttccatcg ctaacttacg gatttcatct gcaacaacag caaaaccttt tcctgccttt
     6781 cctgctctgg cagcctcaat agcagcattt aaagcaagaa gattggtttg ctcactgatt
     6841 cctgcaataa ctttaacaac tttaataact tcttttgttt cttcatttaa aagttgtatt
     6901 tcttgattaa taatatttga tgattctact acattctttg ttttttcatt aagcctgtcc
     6961 attgtactgg atgcataatc ccttgaagct tctgcttttt caatcatctt cataatttct
     7021 tcaattcttt caataacatt attgatattg ttggccaggc ggtccattaa ttgattcgta
     7081 ttttctactt gtctggtttg ctctgaagcc ccttcagcca attcagtaat agcagaagct
     7141 acctgacttg ccgccgcagc agattgttct gaagaacttt ttataatatt ggcaccttta
     7201 acaacttctt gtgatacact gtcggtttta ataattaatt ctcggacatt ttcaatcatt
     7261 ttgttaaagc tataggataa ttttccaatt tcatttttcc cggtaatagg tgacgatacg
     7321 gttaaatctc cttgttccac tttacccatt aagcgaataa tgacatttaa aggcttggaa
     7381 atgctaaatg ccatatacat acctataata atggataaaa taatacaaat aaatactact
     7441 acaataatac cttgtctgac aatttgcatt tcttccatta aaacagacat aggttgcttt
     7501 gtaatcagtt tccatcctgt cttagttgtt gcaaaactga ttacatcatc tttatctcta
     7561 aagtgatcgg attcttttcc tccatatata ttatccaaat atccatccgt taattcactt
     7621 cccagcaagc tactgtcctg atgagaaata acttttctgt tcgtatccaa aaccatgata
     7681 ctggtttcgc cattgatttc tgctgcatca acaatagaac ccagttcttc actatttatc
     7741 aaaaccacaa tgattccaac atcatctgtt gtagaaagtt cttttaccaa aataatatat
     7801 ttgtaagtat tgttataacc tgtaatccag aggaaactct ctttactttc tttttttatt
     7861 ttagctaaat ctttaaatat atccaattca ctggttcctt taagttcact cgttaaattt
     7921 aagcggctgc cagccctgta taaggcacca ctcgttttgt agatataaag tgcttccatt
     7981 cctgcgtttg aaaaattcat tgaaaatagt aatttttgaa tgctttgttc gttttgcttt
     8041 tcttcattaa tcgttgcaaa tttttcccta agaagacttt tcattaattc atcgttacca
     8101 ataatcattc ttggaatttt ttcatattca tcaattttaa aatccagatt aactgccaat
     8161 tgttcaacca ttttttgtga ataataacct actttatttt caacggttgt ttcagcattt
     8221 tcataagcaa aagacgcaat tatacttaat ggaataatac ttaaaataat aaaagtaatg
     8281 atgagctttt gagcaatacc gacatttttc atcggattta aaaatatatc ttttcttttt
     8341 cttttatttg tactctcctt taaaacttta ttatttctta ctttttcaac tttatcttgt
     8401 ttctttaaaa atttaaatcc tttcttctta tcggccataa ctttgccccc caccatttgt
     8461 aaaattactt acccagttgc cagaaacttt acttctaata ctattatata taaaatcttt
     8521 taaaatttct atactatttt cacaaattca aataatg
//
LOCUS       DAOSUD010000082         8413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4388_length_8413_cov_31.444365, whole genome shotgun sequence.
ACCESSION   DAOSUD010000082 DAOSUD010000000
VERSION     DAOSUD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4388_length_8413_cov_31.444365"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG49_09145"
     CDS             <1..317
                     /locus_tag="PLG49_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17513.1"
                     /translation="NEKFFQLFDFDEGRAFEPDFVMLLKEKNHKITIYQVFIEPKGEF
                     LIPYEKWKEDFLKKIEGEARIQEDLIIKAENKQFKLIGLPFYNEKLKKEFEEALINKL
                     YF"
     gene            418..618
                     /locus_tag="PLG49_09150"
     CDS             418..618
                     /locus_tag="PLG49_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17514.1"
                     /translation="MRFNQRKAGTETSAFGSLGRINYDSTPFYTSKLYEGLLKEEITE
                     YVENPVPSEFLDKIFCKTSEKN"
     gene            647..1344
                     /locus_tag="PLG49_09155"
                     /pseudo
     CDS             647..1344
                     /locus_tag="PLG49_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1812..2345
                     /locus_tag="PLG49_09160"
     CDS             1812..2345
                     /locus_tag="PLG49_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17515.1"
                     /translation="MGIDYKNLDAQTRKFMLKELKMDTDKRTLYISPRLNSMGQSIWA
                     ELLKEAIVGHSDDWLATQLNVRGYMKSHEERRLPSGKITVAKVPVNAPEMLAEGEFNR
                     FYARGLCARAIAEGISEVVVCRGKQVRQPRPESQAMIGTRIIPTDLLDDLRRSQGVEP
                     ALGIPPGPNSGLTICLP"
     gene            2478..3296
                     /locus_tag="PLG49_09165"
     CDS             2478..3296
                     /locus_tag="PLG49_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17516.1"
                     /translation="MKEEKIEKIQKRLELITGKWWFFLIFILIQFIIPPYASKGYKLA
                     EQGIVIGEILSHAIVYNHTKLYPIFKIIPVILVISIFFLRNKVTKLFSFYAAISYVLF
                     AFLQNIAVTEKYGLGIVTINFLMFLVVAAFWFWEVIARKNDFAPRKQPVWKYWVVPFA
                     FLAFWYPVNMNTLRPDFNPLYLFTNMAGLAFCMMTPVYLGILTLFYPRVNIAALRVTS
                     LVGIIIALYNIIAIFSNLRVLWWNGVLHIPLLAISIYALVLSFQKISLEETRGD"
     gene            3625..3822
                     /locus_tag="PLG49_09170"
     CDS             3625..3822
                     /locus_tag="PLG49_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17517.1"
                     /translation="MEIKKINKSVRIDLKEKLKEVLPEVFEDNKVNCEKLNSLLSDEV
                     IEKEDDRFYFNWAGKNSQMSR"
     gene            3823..5463
                     /locus_tag="PLG49_09175"
     CDS             3823..5463
                     /locus_tag="PLG49_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOQ17518.1"
                     /translation="MTNKTIIEKKLQEYFEGLKNIYLHKDYTEWTYRTPFENFIKGLN
                     SDYSLVQEPKRDAGLGAPDFKAFNKNRKAGYIETKKLDENLNDILKTDQIERYIASID
                     NLILTNYLQFILIRKGYKVYDCNLFSLSDLDKGHPEISEDKINIFFSLINEFFDYKLP
                     TISSAEELAEELSKKAKLLKELAKEQLLDDLKKVENGENPSSIYDFYQGIKELIKDIE
                     LEDCADAYAQTVTYGLFLAKKGCQNELNIKTASSYIPKSVGVIKRIFLNISGEEFPPN
                     VSWIVEDIINILNATNLDEILTKIDKRGKKDKDPIIFFYEDFLNYYEPEKRKHLGVYY
                     TPRPVVNFIVNSVHSVLKELFDKPLEFADDSVNVLDPAIGTGTFFWIAYLVVLNELKN
                     QGLKGIIRDKIRNHLLKHFYGFELLITPYVISHLKLTYLLQSWHYTFEDDDRIQIYLT
                     NTLEPSDVHGLLPFLREITEESRVANLIKTEKPILVVMGNPPYAGISANKGKWIDDLL
                     KEGYTRADGSKDDGYYRVDGRALEEKNLSGFKMIMSNS"
     gene            5571..6905
                     /locus_tag="PLG49_09180"
     CDS             5571..6905
                     /locus_tag="PLG49_09180"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17519.1"
                     /translation="MQSFDRIYILNLHGNALKKEKCPDGSKDENVFDIKQGVTIGIFI
                     KSKKLGKKRIYYADLWGLKEDKYHWLDRHTIDNVEWQEIKPISPFYFFVPKNTASEEE
                     YNKFWKITDIFPVNSVGVVTSRDDFVIDFDKEELKKRIKDFRNNEIPDDKIKNKFKLK
                     DKKNWKLADARKKVIEDEDWLNSINKILYRPFDIRWIFYDDKVIERSRKEVMRHMLKE
                     NLGLITVRQVKAGEIWYHCWITDEIIESSYISNKTSEINYLFPLYLYSDSGKTPNFSS
                     EFLKFISEKYDKTLTPEEIFYYIYAILHSPTYRKKYEEFLRYDFPRIPFVDNYDKFKR
                     LSNLGKELVELHLMKKRLPIKVKFDIPGSNIVERVKYENGKVWINKEQYFDGVPEDVW
                     NFYIGGYQVLDKWLKSRKNMKMKSEEIEQFFQIIEIVRQTINLMKEIELFCY"
     gene            7071..8357
                     /locus_tag="PLG49_09185"
     CDS             7071..8357
                     /locus_tag="PLG49_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17520.1"
                     /translation="MSSINLPDLPKGKEFEEYISAFFQSGGNYIERNIIEKEVEEILE
                     LDIIATDYDLSKPEIKLIEVKSGDWGFPDLFKVRGWMDYLNIASGVFIASKEKDSVDF
                     LKKKAKGLNIDLVVIPDLNESKKALSELISDGSIEDVDISTWRFSYWVERNLLERLNH
                     KKKFSPDKKCFKSLKDYYSKVNSGIFFTENIIQKVNTLYSTFQEFPRISAKCGNELIG
                     NPFDGEYYTLPKGIYNDTYYKCNYNDIQISTFIEHRARLAILKNAIDYKLYEGAGVTS
                     KTEDTYLDRLPPSFKEGLNTISKHKYFHKYPVFWQWFMWIFGGFVLKDYKSKEYEILS
                     KKTGIPIEEITNAFDSYQILFPQDDGWFMDLSPKSNIKMMKMFPVPFMGIGANYKRLL
                     YTESGKFEDLELTGKHTLNNLIKWNNLTVEVLKNEK"
BASE COUNT         3055 a         1223 c         1626 g         2509 t
ORIGIN      
        1 gaaatgaaaa attttttcaa ctttttgatt ttgatgaagg tagggcattt gaaccagatt
       61 ttgtgatgtt gttgaaagaa aaaaatcata aaattactat ttatcaagtg tttattgaac
      121 caaaaggcga atttttaatt ccttatgaaa aatggaaaga agatttttta aagaaaatag
      181 agggcgaagc aagaatacag gaagatttaa taataaaggc tgaaaataaa caatttaaac
      241 ttataggttt acctttttac aatgagaaat taaaaaaaga gtttgaagaa gctttaataa
      301 ataaattata cttttaaagc aaaagttgag aaactaacat aagttgaaat aaagctacgg
      361 atttaggata aacttaaaaa gattaagttt aattcttaca aaagatgggg tgatttaatg
      421 agatttaatc aaagaaaagc agggaccgaa acaagtgcct ttggatctct cggacgtatt
      481 aactatgatt ccaccccttt ttatacaagt aaactatatg aaggtctact taaagaagag
      541 ataaccgagt atgtggaaaa tccagttcct tctgaattct tagataaaat tttttgtaaa
      601 accagcgaaa aaaattgagg aattgccaga taacagcatt catcttatgg ttacttcacc
      661 gccttataat gtagaaaaag agtatgatga aaacctttct ttaaatgagt ataggaaatt
      721 tctaaaaagg gtatgggatg aagttaaaag agttcttgcc caggtggaag ggtttgcata
      781 aatgttgcca atcttgggag gaaaccgtac attcctctcc atgtttttat tgttgaggat
      841 atgcttgatt tggggtttct aatgcgtggt gaaatcatct ggaacaaagc ctccagtagc
      901 agtccttcaa ctgcgtgggg tagctggttg tccgctaaaa atcctacatt aagagatata
      961 cataaatata ttttggtctt ttctaaaggt atgtttaaaa gggaaaatct cggaagaaaa
     1021 aacacaattt ctaaagagga atttcttgaa tttaccaaaa gcgtgtggac atttgcagca
     1081 gaaccagcga caaaggtggg acatcctgca ccttttccta tagaattacc ttatcgatta
     1141 attcaacttt atacttttga aggtgaaatt gttttagatc catttatggg aagtggtcaa
     1201 acagccattg cttctataaa aagccaacga cattatattg ggtatgacat aaatgaaaag
     1261 tatgtaaaac tagcagaaag gcgtattaag gagttttcct taaattttaa tgctcctaaa
     1321 ttgtttgaat ttgaaggacg atgataaaaa tggcgaaacg gcatataaca caacccaaaa
     1381 ggtccgtgca ttttcaccca aattgccttc ggcaacttct ttttccacaa actgttatac
     1441 ccaattgaaa aatcaaaaag aactggagag caaaaatatt aaaacttgac atcatattca
     1501 agttgattta atattaacca aaagttgtat gtagaaggtg gaaattatta atctaaatga
     1561 aaggatatat gaagtgaatt tcggctataa tgccaaaatg gaaggatata cgaacttgag
     1621 ttcagatata aacaagatag ccaaaatgac gctttgcttg aatacctgcc attaaggggt
     1681 taaggaatgt tagagaaaag agagagactg gtatgcaata tcgaagggaa ataggttgtt
     1741 gcaatggcat tcacatttgg gctatcggga tggcggaata ccaattagaa aattaaaaag
     1801 gaggtaataa tatgggtata gattataaaa atcttgatgc ccagacacgc aagtttatgc
     1861 ttaaggaatt gaaaatggat accgataaga gaaccctata tataagccct cgtttgaatt
     1921 ctatgggaca atctatatgg gctgaacttc tcaaagaagc tatcgttggc catagtgatg
     1981 actggttagc cactcagttg aatgtaagag gttatatgaa atcacatgag gaaaggagat
     2041 taccaagtgg gaaaataacg gttgcaaaag ttcctgtgaa tgctcctgaa atgctcgctg
     2101 aaggtgaatt caatcgcttt tacgcacggg gactatgtgc aagagcaatc gctgagggaa
     2161 tcagcgaagt cgtagtgtgt agaggaaaac aagttcgaca accccgccca gagtctcaag
     2221 caatgattgg aacgagaatt attccgacag atttgttgga tgacctccgt agatcacaag
     2281 gggtagaacc ggctttaggt ataccacctg gaccaaactc gggtttgaca atatgcttac
     2341 catgaaaaac gtaagcacat ccgctaacag cggacatacg actacgcttc gctccgccca
     2401 aatgctcggc ttagcctcgc actttgtatg tccgcatccc gatatacgct atcggctcag
     2461 tagaaggggg tagttgaatg aaagaagaaa aaattgaaaa aattcaaaaa aggttagaat
     2521 taataactgg caaatggtgg ttttttctaa tctttatatt gatacagttt attatccctc
     2581 catatgcttc aaaaggttat aaattagcgg aacagggaat agttataggg gagatactta
     2641 gccatgccat agtatataac cacactaaat tgtatccaat ttttaagata attccagtta
     2701 tcttggttat ctctatattt tttctcagaa ataaggtcac aaaactcttt agcttttatg
     2761 cagctatcag ctatgttctt ttcgcctttc ttcagaacat cgctgtaact gaaaaatatg
     2821 gcttagggat agtcaccata aatttcctga tgtttctggt agttgctgct ttttggttct
     2881 gggaggtcat tgccaggaag aacgactttg ccccacgaaa acagccagtg tggaaatatt
     2941 gggttgttcc ttttgcattt ctagcattct ggtatccagt gaatatgaat acgctgaggc
     3001 cagatttcaa tcctctctac ttattcacaa atatggctgg cctggctttc tgtatgatga
     3061 caccagttta tcttgggata cttactctct tttatcccag ggttaatatt gcagcgctaa
     3121 gggttacaag cctcgttgga ataatcatcg ccttatataa cataatagcc atcttttcta
     3181 accttagagt gctttggtgg aacggagtgt tgcatatacc tttgttagct atatccatat
     3241 acgcccttgt cctttctttt caaaagattt cgttagaaga aacaagaggg gattgaaaag
     3301 atggtaggag cgagccgaca gcgcctatcg gcgggcttct tttatccgta ctccgttatt
     3361 atgaaatgga ggtttcaaaa tgaataaaag gataaaaaat atgagaacaa acaatgctac
     3421 tcaattaacc ttagaaaatt tagaagctat cccacttact tacgggacag tgatattaaa
     3481 taaaaaatag gtgtagtgta aaaatggatt atactcataa tgagtacata cgcgatgcag
     3541 gtgtaactct aattttggat gatatacgca ttgcgtatat gaacaagtta tatgaaattc
     3601 tgaacctcat tatggaggag aaaaatggaa attaaaaaaa taaataaatc ggtaagaatt
     3661 gatttaaaag aaaaattgaa agaggtatta cctgaagttt ttgaggataa taaagtcaat
     3721 tgtgagaagt tgaactcctt attaagtgat gaggtcattg aaaaggaaga cgatagattt
     3781 tatttcaatt gggctggaaa aaattctcaa atgagtagat aaatgactaa caaaaccata
     3841 attgaaaaaa aactacaaga gtacttcgaa gggcttaaaa atatatactt acataaggac
     3901 tatacagaat ggacttatag aactcctttt gaaaacttta ttaaaggatt gaattccgat
     3961 tatagtcttg ttcaagaacc aaaaagggat gccggacttg gtgctcccga ttttaaagct
     4021 tttaataaaa atagaaaggc aggttatata gaaactaaaa aacttgacga aaatttaaat
     4081 gatatactta agaccgacca gatcgagaga tatattgcaa gtatagataa tctaatactc
     4141 accaattatc ttcaatttat tctcattaga aaaggttaca aagtttatga ttgcaatctt
     4201 ttttcactat ctgacttgga taaagggcat ccggaaattt ctgaagataa aatcaatata
     4261 ttcttctctc taataaatga attttttgat tacaaacttc caaccataag ctccgctgaa
     4321 gaattagctg aagaactctc taaaaaagcg aaattattaa aagaactggc taaagaacaa
     4381 cttttggatg atttgaaaaa ggttgaaaat ggcgaaaatc cttcatcaat ttatgatttt
     4441 tatcaaggta taaaggaact tataaaagat atagaattag aggattgtgc agacgcctat
     4501 gcacaaactg ttacttatgg cttgttcttg gctaagaaag gttgccaaaa tgagttaaat
     4561 ataaaaacag catcatccta tatccccaaa agtgtcggtg taattaaacg tatcttcctg
     4621 aatatatcag gtgaggagtt tcctccaaat gtctcgtgga ttgtagaaga tattataaat
     4681 attttaaacg ctacaaattt agatgaaatt ttaactaaga ttgataagcg tggtaaaaaa
     4741 gataaagatc caataatatt tttctatgaa gattttttga attattacga gcctgaaaag
     4801 agaaaacatt taggagttta ttatactcct cgtccggttg tcaacttcat tgtaaattct
     4861 gtgcattcgg ttttgaagga gctatttgat aaaccattgg aatttgcaga cgatagcgta
     4921 aatgttctcg atccagcaat aggaacagga acattcttct ggatagcata tcttgttgtt
     4981 ttaaatgagt taaaaaatca gggattaaaa ggaataatta gagataagat cagaaatcat
     5041 cttctaaagc atttttatgg gtttgagctt ttaattactc cttatgtaat ttctcatttg
     5101 aaacttacgt atttactcca gagttggcat tacacctttg aagacgatga ccgaatacaa
     5161 atctatctta caaacacatt agagccatca gatgttcatg ggctcttacc gttcttaagg
     5221 gagataacag aagaaagcag ggttgccaat ctgataaaga cagaaaaacc aattctcgtt
     5281 gtgatgggaa atcctccata tgcaggtata tccgcaaata aaggaaaatg gattgatgat
     5341 ttgctgaaag aaggatatac acgagcagat ggaagtaaag atgatggcta ctacagggtg
     5401 gatgggagag cacttgaaga gaaaaatcta agtggcttca agatgattat gtcaaattca
     5461 taagatatgc acaatggaag atagacaaaa atggagaggg ggttgttggt tatataacga
     5521 atcatagtta tctggataac ccaacattca ggggaatgag aggatcacta ttgcagagtt
     5581 ttgacagaat ttacatctta aatttacatg gaaatgcttt aaagaaagaa aaatgtcctg
     5641 atggtagcaa agatgaaaat gtctttgata taaaacaagg agttacgatt ggaatattca
     5701 taaagagtaa aaaattaggc aagaaaagaa tttattatgc agacctttgg ggattgaaag
     5761 aagataaata tcactggtta gataggcata caatagataa tgttgaatgg caagagataa
     5821 agccaatatc accattctat ttctttgttc ctaaaaatac tgcgtcggaa gaagaatata
     5881 ataaattctg gaagattacg gatatttttc ctgtaaacag tgttggagta gtaacttcaa
     5941 gagatgactt tgtaatcgat tttgataagg aagaacttaa aaaacgcata aaagatttca
     6001 gaaataacga aatacctgat gataaaataa aaaacaaatt caaattaaag gacaaaaaga
     6061 attggaaact ggcagatgca agaaagaaag taatagaaga tgaagattgg ttaaattcca
     6121 ttaataagat actttataga ccttttgata ttcgctggat tttttatgat gataaagtta
     6181 tcgaacgatc tcgtaaagag gttatgagac acatgctaaa ggaaaatttg gggctgataa
     6241 ctgtgagaca ggtaaaagca ggcgagattt ggtatcattg ctggattacc gatgaaatca
     6301 tagaaagtag ttatatttca aataaaacaa gcgaaataaa ttatctattc ccactctatc
     6361 tttattcaga ctctggcaaa accccaaact tcagctctga atttttgaaa tttatctcag
     6421 aaaaatatga taaaacttta acgccagaag agatatttta ttacatctat gctatcttac
     6481 attctccaac ttacagaaaa aagtatgagg agtttttacg atacgatttc ccgagaatac
     6541 cttttgttga taattatgat aaattcaaaa gactttctaa tctgggtaaa gaactggttg
     6601 agttgcattt gatgaaaaag aggttgccga taaaagttaa atttgatatc cccggctcaa
     6661 atattgttga aagggtgaaa tacgaaaacg gtaaggtatg gataaacaag gagcaatact
     6721 ttgatggtgt gcctgaagat gtctggaatt tctatatcgg tggttatcag gtcttagata
     6781 aatggcttaa aagcagaaaa aatatgaaga tgaaaagcga agagatagaa caattttttc
     6841 aaattataga gattgtaaga cagacaatta atttgatgaa agaaattgaa ctgttttgct
     6901 attaaggcac catgatacag tcattccctg ttaaatattg cttctccctt tatccatcgg
     6961 gtcatataat agcagataaa atggaaagaa aaaattatcc aaatcacctt cggcaacttc
     7021 ttttatccac aaaacgttat ccgaaatagc taatacaaag gaggcgaaag atgtcatcta
     7081 ttaatttacc tgatttgccg aaagggaagg agtttgaaga atatatttcc gctttttttc
     7141 agtccggtgg gaattatatt gaaaggaata tcattgaaaa ggaagttgaa gagattttgg
     7201 agttagatat tattgccaca gattacgact tatctaaacc tgaaatcaag cttatcgaag
     7261 taaaatcagg ggattggggg ttcccggatt tatttaaagt gcgggggtgg atggattatt
     7321 taaatatagc tagtggagta tttattgcta gtaaagaaaa agacagcgta gattttttaa
     7381 aaaagaaggc caaaggacta aatatagatt tagtagtaat tcctgactta aacgagtcca
     7441 aaaaagcatt atctgaactt ataagtgatg gaagtattga agatgtggat atttcaactt
     7501 ggagattctc ttattgggtt gaacgtaatt tattggaacg tctaaaccat aagaaaaaat
     7561 tctcaccaga taaaaaatgt tttaaatctt tgaaagatta ctattctaag gtaaatagtg
     7621 ggatattctt tactgaaaat attattcaaa aagtaaatac attatactct acctttcaag
     7681 aattcccacg catttcagct aaatgtggaa acgaacttat aggtaaccct tttgatggtg
     7741 aatattatac cttaccgaaa gggatctaca atgatactta ctacaaatgt aattataatg
     7801 atattcaaat atctaccttt atagaacata gagcaagatt ggctatattg aaaaatgcga
     7861 tagattataa actttatgaa ggtgctggag ttacgagtaa aaccgaggat acctatttgg
     7921 atcggttacc tccctcattt aaagaaggct taaatactat ttctaaacat aaatatttcc
     7981 ataaatatcc tgtcttctgg caatggttta tgtggatatt tggaggattt gtcttgaaag
     8041 attataaaag caaagaatac gaaatcttat ctaaaaaaac tgggattcct atagaagaaa
     8101 taacaaatgc ttttgattct tatcaaatac tatttccaca agatgacgga tggtttatgg
     8161 atttatctcc aaaatcaaac ataaaaatga tgaaaatgtt cccagttcct tttatgggaa
     8221 tcggtgcaaa ttataaaagg ttgctctata ccgaatctgg aaaatttgaa gatcttgaac
     8281 ttactggaaa acatacctta aataatttga ttaaatggaa taatttaaca gtagaagtgt
     8341 taaaaaatga aaaataagca acttcgccta acacagcata cacgctgcgg gctgacgccc
     8401 ttgcccttcg gta
//
LOCUS       DAOSUD010000083         8283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4449_length_8283_cov_20.131624, whole genome shotgun sequence.
ACCESSION   DAOSUD010000083 DAOSUD010000000
VERSION     DAOSUD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_8283_cov_20.131624"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLG49_09190"
     CDS             <1..478
                     /locus_tag="PLG49_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17521.1"
                     /translation="IRQMPALLQKLPDAKFLVVGDGPVKKDLEELCKELNIEESVIFA
                     GEQPWDSIGKFYQIGDVFVSASVTETQGLTFAEAMAAKIPVVAKQDKSIEGIIQDHTN
                     GRVFAEDEELSSILFELLTQKDVAMQLTENAAAMVEPLSSTHFGENIEKIYQSLVK"
     gene            complement(495..1340)
                     /gene="mltG"
                     /locus_tag="PLG49_09195"
     CDS             complement(495..1340)
                     /gene="mltG"
                     /locus_tag="PLG49_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ17522.1"
                     /translation="MEIIKKWVIKHYLWLMLGCSILAIGFAGVTIYQVYQLMTLNVPT
                     ISQKSFKIIPKHQEEDIRNAITLNVCAGDTVEKISQKLEKAGIISRNDFFTYLKQGQI
                     NLEGIVFEGEYSIPVPSTPKEIHSILTEPYAQWIKNESKKFISLGRTPVEIITIASMI
                     EKEAAEDSERPIIAGVIYNRLKNNMKLQIDATVIYALGEHKSRLTYEDLKVNSPYNTY
                     IIEGLPPGPICTPRKASIEAALNPDMHSYFYYVLSSYGSQKHLFAETYEEHLSNVEKY
                     KTTVK"
     gene            complement(1383..1754)
                     /locus_tag="PLG49_09200"
     CDS             complement(1383..1754)
                     /locus_tag="PLG49_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ17523.1"
                     /translation="MILFVIGLLSGLISGMGIGGGTVLIPALTIFVGMSQHSAQSINL
                     IYFIPTAIAALVIHIRNKRIRKELLWFLIGGGVVGSVIGSFVAISLDAVLLRKLFGVF
                     LFYMGIAEIRKNKRKIKENRE"
     gene            complement(1751..2110)
                     /locus_tag="PLG49_09205"
     CDS             complement(1751..2110)
                     /locus_tag="PLG49_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ17524.1"
                     /translation="MVKEKFKIILVGVITGLTNGLFGAGGGTIVVPAMERILKVEEHE
                     SHATAIAVILPLSMMSTFIYVRHQILDWKSLLYIAVGGMAGGILGAKLLSRISPQWLH
                     KIFGIFMIVASIRMIMG"
     gene            complement(2205..2873)
                     /gene="nth"
                     /locus_tag="PLG49_09210"
     CDS             complement(2205..2873)
                     /gene="nth"
                     /locus_tag="PLG49_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ17525.1"
                     /translation="MNLYGDKSKSKKILALLDEYYPKDIKCYLNYETPWQLLIATILS
                     AQCTDDRVNMVTKDLFKKYPSLLDFAEADLEELEQDIKSTGFYRNKAKNIILCAQKLL
                     EKYKGEVPKNIDELTQLAGVGRKTANVILGNIYGIPSIVVDTHVKRVSYRLGLTKFTD
                     PTKIEFDLMKILPEEHWIRYNTQIIAHGRAICTARNPKCEKCFLLPYCQYGLEERMGA
                     VSER"
     gene            complement(2954..4717)
                     /locus_tag="PLG49_09215"
     CDS             complement(2954..4717)
                     /locus_tag="PLG49_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOQ17526.1"
                     /translation="MNIVLSALNAKYIHTSLALRSLKSFCTEYKSNITIAEYTINHEE
                     NYIMNELYKLKPDILGFACYIWNIEQTLDLVINIKKILPDTLIILGGPEVSYDGEKLL
                     KEYKEIDIIVYGEGEATFYDLLHSFIDGDRSLERIDGIIYSSNGKIVKNKERTPLNLD
                     AIPFVYEDFKDMENQILYYESSRGCPYQCQYCLSSIDKKVRFLSLERVYSDLQRFLDG
                     RVKQVKFVDRTFNCNKNHARAIWNYLIEHDNGTTNFHFEISADLLDDEIIAFLSKARP
                     GLFQFEIGVQTTNREVLKIINRKMDFERLKTVVKNIKKGQNIHQHLDLIAGLPGENYA
                     SFRNSFNDVYNLFPEQLQLGFLKVLKGSGMRENEEKYGLVYKSKAPYEILFTKELNYE
                     EMLKLKMIEEMVEKYYNSGRFYYSIRYITSFFDTPFDFYEALAIFWERKGYHHVQHNK
                     IQLYTILLEFFKEKVEEEAEVLKALMKFDIYLLEKAKKLPEWMNSSEEMYKSQIRDFY
                     RNEENIKCYLPKLIEYSSKQISRMAHLEIFSVDFTEWIKSIGSGEESPSIDKKTTAIL
                     FDYYSKNQILGHAAYYKVSIQ"
     gene            complement(4832..6172)
                     /locus_tag="PLG49_09220"
     CDS             complement(4832..6172)
                     /locus_tag="PLG49_09220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOQ17527.1"
                     /translation="MVLLADLRKHINIFFILLILAGILLFFQHYNLLLVDVQDDIAVI
                     RVNFLVPMEQFDIEKNIEIKSSLPNTKFRCDLKWEDHNRLKVTITELSELKGQKILFK
                     IHGAKSQFPFLHKSLSTSVQFQTEPKLLEVENKDNVPTEGPLVLQFNTPMDFRNIHKF
                     IQSDIKFDLKTHEFEENGKSTDYSRWNLYPKEKMKNESNYLLLIKKGLKAQCGHMLKE
                     DIQIQIRTASKPKIVETTPKQSAQWVGLYPKITAKMSEELKDAIVMIDHVKQKVSIKE
                     NKLEFLPHTILNPGEEYEVSIQGISKYGEKTDPYILKFITMPINDDDLWVEVSLKDKH
                     EVIIHKGKEIIRRMPASGGTKEEPTVLGTFYIQDRGTSFFSKRFNEGATYWVRIIDQY
                     LFHGIPRDNHWNIIESERKKIGKPASHGCVRLNEEDAKWFYENIPHGTMVIIHE"
     gene            complement(6333..7580)
                     /locus_tag="PLG49_09225"
     CDS             complement(6333..7580)
                     /locus_tag="PLG49_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOQ17528.1"
                     /translation="MIKIRDYKKILCIYKDVFLLDLTAIGIGIIVGIIDTLFGKVLLE
                     ITNIRNSYVFQLIPFLPLTGIVILFTYFRIGKNSIKGMSLIFSAGLNDEETIPKRLVP
                     LVIVSTWLTHLFGGSAGREGVAVQIGGTVGYSIGRELNIRNSSKILLITGMAAGFAGL
                     FQTPIAAIFFAMEVLTAGVVEYSALFPCILGAFTASYMSHTLGLEKFDVNLNYSLEFN
                     IYLFLKLVLLGIFFGLIGGFFANLLNWSKKFFAQRIGDPILRIFILGCILSVFLLILH
                     KGRYAGLGTNLINASFSNEKIYEYDWLLKFLLTILTLSAGFQGGEVTPLFSIGASLGA
                     VLSPVMGLPIEFTAALGYVAVFGGATNTILAPIFIGGEVFGYNYLPYFFIVSAVAYVF
                     NGNKSIYSAQKILGDVKKVISLE"
     regulatory      complement(7762..7836)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7926..7997)
                     /locus_tag="PLG49_09230"
     tRNA            complement(7926..7997)
                     /locus_tag="PLG49_09230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7963..7965),aa:Gly,seq:gcc)
     gene            complement(8006..8079)
                     /locus_tag="PLG49_09235"
     tRNA            complement(8006..8079)
                     /locus_tag="PLG49_09235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8045..8047),aa:Gly,seq:tcc)
     gene            complement(8084..8156)
                     /locus_tag="PLG49_09240"
     tRNA            complement(8084..8156)
                     /locus_tag="PLG49_09240"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8121..8123),aa:Phe,seq:gaa)
BASE COUNT         2638 a         1612 c         1233 g         2800 t
ORIGIN      
        1 gattcgtcaa atgcctgcat tacttcaaaa acttcctgat gcaaagtttt tagttgtagg
       61 agacggccct gtaaaaaaag acttagaaga attgtgtaaa gaactgaata ttgaagaatc
      121 tgttattttt gccggtgaac agccgtggga ttctatagga aagttttatc agataggaga
      181 tgtttttgtc agcgcttccg taacagaaac ccaaggactt acctttgctg aagctatggc
      241 tgctaaaata cctgtcgtag caaaacagga caaaagtata gaaggaatca tacaagacca
      301 tactaacgga agagtatttg ctgaagatga agagttatct tctattcttt ttgagttatt
      361 aactcaaaaa gatgttgcta tgcagctgac tgaaaatgct gctgcaatgg tggaaccact
      421 ttcttctact catttcggag aaaatattga aaaaatctat caatctttag taaaataggg
      481 aagcttaagc ttccctattt tactgttgtc ttgtattttt caacattact taaatgttct
      541 tcataagtct ctgcaaaaag atgtttttgg cttccatatg aacttaaaac ataataaaaa
      601 taagaatgca tatcagggtt taaagctgct tcaatagaag cttttctcgg agtacagata
      661 ggccctggtg gaagcccctc aataatgtat gtattatatg gagaattaac ctttaaatct
      721 tcatacgtaa gcctggattt atgttctcct aaagcataaa tcactgtagc atctatttga
      781 agcttcatat tatttttcaa tcgattataa atgactccag caatgatcgg tctttcactg
      841 tcttcagcag cttctttttc aatcatagaa gcaatcgtta taatttctac aggtgttcgt
      901 cctaagctaa taaatttctt tgattcattt ttgatccatt gggcataggg ttctgtaaga
      961 atagaatgta tttctttagg agttgaaggc acaggaatag aatattctcc ttcaaataca
     1021 ataccttcta aattgatttg accttgtttt aaatatgtaa aaaaatcatt ccgtgaaata
     1081 atccctgctt tctctaactt ttggcttatt ttttctactg tatctcctgc gcatacattt
     1141 agagtaattg cattccttat gtcttcttcc tgatgtttcg gaataatctt aaaagacttt
     1201 tgagaaatgg ttgggacatt tagtgtcatc aattgataaa cttgataaat cgttacaccg
     1261 gcaaatccta ttgccaatat ggaacatccc agcataagcc acagatagtg cttaatgacc
     1321 catttcttta ttatttccat aatttcaccc actatttttt cagcttttga tttttattat
     1381 ctttattctc tgttctcttt aattttcctt ttgttctttc taatctccgc tatccccata
     1441 taaaaaagaa atactccaaa caatttccgc aaaagtacag catctaaaga aattgccaca
     1501 aaagatccaa taacagaacc cacaactcct ccgccaatca aaaaccaaag taattctttt
     1561 cttattcttt tatttcgtat gtggatcacc agggctgcaa tcgccgttgg gataaagtaa
     1621 atcaaattga tgctttgagc actatgctga ctcataccca caaagattgt taaggcagga
     1681 attaaaaccg ttcctccacc aattcccatc ccgcttatga gtcctgacag caaacctata
     1741 acaaataaaa tcatcccata atcatcctta tcgaagcaac aatcataaat ataccaaaga
     1801 ttttatgaag ccattggggg gaaattctgc tcaataactt tgcccccagt attcctccgg
     1861 ccatcccccc tacggcaata tacagtagcg atttccaatc caatatttga tgtcttacat
     1921 aaataaatgt actcatcata gataaaggta aaatcactgc aatagctgtt gcatgagatt
     1981 catgttcctc aacttttaaa atcctttcca tggcaggaac tacaattgtt cctcctcccg
     2041 ctccaaaaag gccattggtt aaaccagtaa ttactcctac caaaataatc ttgaattttt
     2101 ccttaaccaa aaaggttgcc tccattaatt taaattttta tacttacttt cccctgtttg
     2161 gcataatttc attacatgta ccttaaaaaa attacactga acatttacct ctcagaaacc
     2221 gctcccattc tctcttctaa tccatactga cagtatggaa ggagaaaaca tttttcgcat
     2281 ttgggatttc ttgcagtaca gatggctcgt ccatgggcaa tgatctgagt attatatcga
     2341 atccaatgtt cttcaggaag aatcttcatt aaatcaaatt caatttttgt aggatctgta
     2401 aatttggtaa ggcctagtct ataagaaacc cgtttgacat gagtatctac aacaatactg
     2461 ggaatgccat aaatatttcc caaaataaca ttggctgttt ttcttcctac tcccgctaat
     2521 tgagtaagtt catctatatt ttttggaact tcccccttat atttttccag taatttctgc
     2581 gcacagagaa taatgttttt ggccttattt ctataaaatc ccgtggattt tatgtcttgc
     2641 tccaattcct ctaaatcagc ttcggcaaaa tccaaaagtg atggatattt cttaaataaa
     2701 tcttttgtaa ccatatttac ccggtcatcg gtacattgag cacttaatat tgtagcgatc
     2761 aacagctgcc agggtgtttc ataatttaaa taacatttaa tatctttagg ataatattca
     2821 tctaaaagtg ctaagatttt tttacttttg cttttatctc catataaatt cacctcatca
     2881 tttttttggg aaataattta taaatttcca tattatttcc tgggaaatta tatctttatt
     2941 tgggaaatat tgtttattgg attgatactt tgtaatatgc agcgtgcccc aaaatttgat
     3001 tttttgaata ataatcaaac aagatcgcag tcgttttttt atctatactt ggtgattctt
     3061 cgccacttcc gatactttta atccattctg taaaatcaac agaaaaaatc tctaaatggg
     3121 ccattcggct gatctgtttt gatgaatatt ctattaattt tggtaaataa catttgatat
     3181 tctcttcatt tcgataaaaa tcccttattt gagatttata catttcttca ctggaattca
     3241 tccattccgg caatttttta gccttctcga gtaaatatat atcaaatttc atcaacgctt
     3301 taagtacttc tgcctcttct tccacttttt ctttaaaaaa ctctaataat atggtataga
     3361 gttggatttt attatgctga acatgatgat atccttttct ttcccaaaaa atagccaaag
     3421 cttcataaaa atcgaagggc gtatcaaaaa aagaagtgat atatctgatg ctgtaataaa
     3481 agcggcctga attataatat ttttctacca tttcttcaat cattttaagt tttagcattt
     3541 cttcataatt taattctttg gtaaacagga tctcataggg ggctttgctt ttataaacaa
     3601 ggccatattt ctcctcgttt tctctcatgc cggagccttt taagactttt aaaaatccta
     3661 actgaagctg ttcaggaaat aaattataaa catcattaaa agaattccta aaggaagcat
     3721 aattttctcc tggaagccct gcaatcaaat ccaaatgctg atggatattt tggcctttct
     3781 taatattttt cactaccgtt tttagacgtt caaaatccat cttacgattg atgattttta
     3841 gaacttctct gttggttgtt tgaacaccta tttcaaattg gaataatcca ggccttgcct
     3901 tagataaaaa agcaataatt tcgtcatcta ataaatctgc ggaaatctca aaatggaaat
     3961 tggttgttcc gttatcatgt tctattaaat aattccagat tgctcttgca tgatttttat
     4021 tgcaattaaa ggttctgtcc acaaacttaa cctgttttac tctcccgtct aaaaatcgct
     4081 gtaaatcgga atataccctt tcaagagaca aaaatcttac tttcttgtct attgaagaca
     4141 ggcagtattg gcattgataa ggacatcctc tgctgctttc ataataaaga atttgatttt
     4201 ccatatcttt aaaatcttca tacacaaaag ggatagcgtc taaattcaga ggggtacgtt
     4261 ctttgttttt aacgatcttc ccattggaag aatatatgat tccatctatt ctttccaggg
     4321 acctgtcccc atctataaaa ctgtgaagga gatcgtaaaa agttgcttct ccttctccat
     4381 aaacaatgat atctatttct ttatactctt ttagcagttt ttctccatca tatgatactt
     4441 caggtccccc taatataatg agggtatctg gcaatatttt tttaatattg atcaccaaat
     4501 ctaaagtctg ttcaatattc catatataac atgcaaatcc caatatatcg ggtttaagct
     4561 tgtacaattc attcataatg taattttcct catgattaat ggtatactct gcaatggtga
     4621 tgttggactt atattctgta caaaaagatt taagcgatct aagggctaaa gatgtatgta
     4681 tatatttggc gttcaatgca gacaaaacaa tattcataat aattctccat tctttatcat
     4741 ttgttttatt atagtataaa ttctcatatt atgaaagtag agtgtcgtaa ccgacactcc
     4801 atcaaaatag ctttaactta tttaatgcaa tttactcgtg gataataacc attgtcccat
     4861 gaggaatatt ttcataaaac cattttgcat cttcttcatt caatcgcaca catccgtgac
     4921 ttgccggctt acctattttc ttacgttccg attctatgat attccagtga ttatctctgg
     4981 gtatgccatg gaataaatat tggtcgataa ttcttaccca ataggttgca ccttcattga
     5041 aacgtttcga aaagaaactg gtacctctgt cctgaatata aaaagtccca agtactgtag
     5101 gttcttcttt ggttccaccg gaagcaggca tccttcttat gatctctttt cctttatgga
     5161 tgattacttc atgcttatct tttaaagaaa cctctaccca tagatcgtca tcattgatag
     5221 gcatagtaat aaactttaat atataaggat cggttttttc accatatttt gaaatgcctt
     5281 gaatactcac ttcatattct tctcccggat ttaaaatcgt atgaggtaga aattccaatt
     5341 tattttcttt gatagatact ttttgcttta catgatcaat cataacgatg gcatccttta
     5401 attcttcact cattttggca gtaattttag gatataaccc cacccattga gcagactgtt
     5461 tgggagttgt ttccacaatc ttaggtttgg aagcggtacg gatttgaatt tgaatatctt
     5521 cttttagcat gtgtccacat tgagctttta atcccttttt gattaaaagc agataattgc
     5581 tttcattctt cattttttct ttgggataca agttccatct gctgtaatcc gtgctttttc
     5641 cattctcttc aaattcatgg gtttttaaat caaacttaat atcagattga ataaatttat
     5701 ggatatttct aaaatccata ggagtgttaa actggagtac aagtggccct tccgtgggaa
     5761 cattgtcttt attttctact tctaatagtt ttggttctgt ctgaaactgt acggaagttg
     5821 acaaagattt atgtaaaaaa ggaaattgtg attttgcacc atgaatttta aataaaatct
     5881 tttgcccctt taattcactg agttcagtta tagtaacttt taggcgatta tgatcctccc
     5941 attttaaatc acatcggaat tttgtattgg gaagggagga tttaatttca atattttttt
     6001 caatgtcaaa ttgctccata ggaactaaaa aattaactct tataactgca atatcatctt
     6061 gtacatctac aagcagtaaa ttataatgtt gaaaaaatag caatattcct gccaaaatca
     6121 ataatatgaa aaagatattg atatgctttc gcaagtctgc aagcaaaacc atgctttgac
     6181 cccctctgtc atttattctt aactaaatat taagacttgc ttactttttt agtactaata
     6241 aatgtacaag ccaggcttat ttcttctcat taatgtatat gttttttaaa aatgatatag
     6301 tacattcaaa aaggccatat catcagaaca ttttattcta atgatatgac cttttttaca
     6361 tctcctaaaa ttttttgcgc agaataaata gacttatttc cattaaaaac ataagcgact
     6421 gccgaaacta taaaaaaata aggcaaataa ttatatccaa acacctctcc cccaataaaa
     6481 atcggagcaa gtattgtatt cgtagcacca ccaaatactg cgacatagcc aagcgctgct
     6541 gtaaattcaa tgggcaatcc cataacaggc gagagcactg ctcccaaact tgcacctata
     6601 gaaaaaagtg gcgttacttc ccctccttga aaaccagcac tcagagttaa aatagtaagc
     6661 agaaatttta gtaaccaatc gtattcatat attttctcat ttgaaaaaga tgcattgatc
     6721 agatttgttc ctaaacctgc atatcttcct ttatgcaaga tcagaagaaa tacacttaaa
     6781 atacacccca aaataaatat tcttaaaatc ggatccccaa tacgttgagc aaaaaacttt
     6841 ttggaccaat tcaatagatt agcaaaaaag cctccaatta acccaaaaaa aatacccagc
     6901 aaaacaagtt ttaaaaatag atatatatta aactccaatg agtagttcag attcacatca
     6961 aatttttcaa gacctaaagt atgtgacata taacttgcag taaaagcccc caaaatacaa
     7021 ggaaacaaag cagaatattc aacaacgcca gccgtcaaaa cttccatagc gaaaaaaata
     7081 gcggctatag gtgtctgaaa cagtcctgca aatcctgctg ccataccggt tataagtaaa
     7141 atcttggaac tatttcttat gttgagctct cttcctatcg aatagccaac tgttccacct
     7201 atttgaactg ctactccttc acgaccagca ctacctccaa atagatgggt cagccatgta
     7261 ctcactatca ccaacggtac taatctcttt ggtatagtct cctcgtcatt taatcctgct
     7321 gaaaaaataa gtgacattcc ttttatgcta ttcttgccta tcctgaaata agtaaacagg
     7381 atcactatcc ctgtcaacgg aaggaagggg attaattgaa aaacatagct gtttcttata
     7441 ttggtaatct ctaatagaac tttaccaaaa agggtatcaa ttatacctac aataattcct
     7501 attccaatcg ctgttaaatc cagtaaaaat acatctttat aaatgcacaa tatcttttta
     7561 taatctcgta tcttgatcat tcctttcttc ttcaaaaaag aaaaatttct gcttgcaaaa
     7621 ctctcgtcgc cgagcgcggt cggcaaaacc gacagaatac aacactacaa cacgcgcaag
     7681 tttcatcctg ctcgaaaggc tttttattgt atgggaaatc tagaggaatt tcctatgcaa
     7741 taaaaaagct aataactctg ttgttcaaca gaagtcatta gctttaaaag cggtttaggc
     7801 ttaataccaa gggagaactt cattcccttt cataattata tcaaataacg aaaacataat
     7861 catttataaa agtataaaag aataaaaaca taagcctata aattacaaga cttatgtttc
     7921 agataagcgc gagacgggat tcgaacccgc gaccctcgcc ttggcaaggc gatgctccac
     7981 cactgagcca ctcgcgcaca atttttggag cgggtgatgg gaatcgaacc cacgtatcca
     8041 gcttggaagg ctggtgttct accattgaac tacacccgca cagtgcccag aaccggaatc
     8101 gaaccagtga cacgaggatt ttcagtcctc tgctctaccg actgagctat ctgggcattt
     8161 atgtattttt aaaatatgcc ggagaccgga atcgaaccgg tacggtcggt aaggaccgca
     8221 ggattttaag ttctatcagt caaaaagaac ctttaggaac ggagtggaac gattaggaat
     8281 tta
//
LOCUS       DAOSUD010000084         8032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4589_length_8032_cov_25.202582, whole genome shotgun sequence.
ACCESSION   DAOSUD010000084 DAOSUD010000000
VERSION     DAOSUD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4589_length_8032_cov_25.202582"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..925)
                     /locus_tag="PLG49_09245"
     CDS             complement(179..925)
                     /locus_tag="PLG49_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ17529.1"
                     /translation="MIRVLLVEDNDYERQRLVQIINANTEGIKVYEAEKGSTAINIIK
                     NHDIDLFLMDIELPDISGIKLAEKIRKVPKYEFAHMVFITTHGYLLLDAFKKIHCYDF
                     IVKPYENKEIVDVINKLTRAVIRQKKEHVQKREEIRFKLKSCILRIYVDEILFIESQG
                     RNCIIHTKKKEYEISNYNMSKMLSILPQNYFMQTHKSYIVNINNIREVDKRERNSWTV
                     FFDDYDIPAFVGNTYKNHFIERTNDVRVVE"
     gene            complement(1110..1268)
                     /locus_tag="PLG49_09250"
     CDS             complement(1110..1268)
                     /locus_tag="PLG49_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOQ17530.1"
                     /translation="MSQWVCSVCGYVYDPAEGDPDNGIAPGTAFEDLPEDWVCPVCGV
                     GKDEFEEQ"
     gene            complement(1440..3203)
                     /gene="pyk"
                     /locus_tag="PLG49_09255"
     CDS             complement(1440..3203)
                     /gene="pyk"
                     /locus_tag="PLG49_09255"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOQ17531.1"
                     /translation="MRKTKIVCTLGPATSDVDTIKKLIKSGMNAARINFSHGTYESHG
                     KIIDNLIKAREELNIPIPLLLDTKGPEIRIKDFVKEPVFLQQGQTFTLTTENVEGDET
                     RVSVTYKNLPFDLRRGSRVLIDDGLIELRVKNLTDTEVECEVINGGPLSSKKGVNIPD
                     VYVNLPSLTEKDIEDIKFGIKKGFDYIAASFIRSASDVIKIRRILEENGGNDIDIIAK
                     IENRDGVDNIDEILEVADAIMVARGDLGVEIPIEEVPLVQKMLIKKANQMGKPVITAT
                     QMLDSMVRNPRPTRAEANDVANAIFDGTDAIMLSGETAKGAYPIESVLMMATIAERTE
                     SSIDYIKNMTKFHTSIQSNITDAISHATCTTAADLNAACIVTVTRSGGTARMVSKFRP
                     CAPVLACTVDEKAWRQLNLVWGCIPVKTEFKHTTDELFNQAVEKSVETGLAKNGDIIV
                     ITAGVPVGVSGTTNILKAHIVGNVLVKGKGIGKKTVSGTARVIKLIEEADKYFKKGDI
                     LVTSNTDNDFLPYIKKASAIIVEEGEHGENSHAAIVGRALDIPVIIGAKNAVDLIRDG
                     TLITVDSKNGLVYNGAQEDVQ"
     gene            complement(3253..4227)
                     /gene="pfkA"
                     /locus_tag="PLG49_09260"
     CDS             complement(3253..4227)
                     /gene="pfkA"
                     /locus_tag="PLG49_09260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ17532.1"
                     /translation="MSKKISTIGVLTSGGDAPGMNAAIRSVVRTAISKGIKVMGVRKG
                     YNGLINGDIFEMNTKSVSDTIQRGGTILQTARCAQFMTEDGQRRAAEMCRVFGMEGLV
                     VIGGDGSLQGAEKLSKLGINTIGIPGTIDLDIACTDYTIGFDTAVNTAMEAINKIRDT
                     STSHERCSIVEVMGRHSGYIALWSAISNGAEMVLIPEKERPSDEEIVKEILESKKKGK
                     KHFLIIAAEGVGGSEQLAKKIEELTGIETRASILGHLQRGGSPSALDRLHASMMGAIA
                     VELLCEGKSNRVIAYRDGKYVDFDINEALQMKKTINEKMYEVSKILSL"
     gene            complement(4373..7825)
                     /locus_tag="PLG49_09265"
     CDS             complement(4373..7825)
                     /locus_tag="PLG49_09265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ17533.1"
                     /translation="MPFTHLHVHTEYSLLDGSAKIEELIARTKELGMDSIAITDHGAM
                     YGVIDFYKEAKKQGIKPIIGCEIYVAPRSRFDKEKQDANYYHLVLLAENMEGYKNLIK
                     LVSYGFIEGFYHKPRVDLELLEKHSKGLIGLSACLAGPVARAIREESYEKAVEVALQY
                     ERILGKGNFYLELQDHGLKEQGEVNQCLVRMSKETGIPLVATNDIHYIYKEDSKAHEI
                     LLCIQTAKTINDEDRMVYPGGEFYLKSPEEMYHRFPYAKEALRNTYEIAQRCNVEFTF
                     HELKLPEYDVPEGYTAKEYLRKLCYEGFNDRYPDADDELKARLEYELDTIERMGYVDY
                     FLIVWDFIKYAKDNGIMVGPGRGSAAGSMVAYCLQITNIDPIKYQLLFERFLNPERVS
                     MPDIDIDFCYERRQEVIDYVIRKYGEDRVAQIITFGTMSARAVIRDVGRAINMPYAEV
                     DKVAKMIPAELGITIDRALEINGELKALYENDEEVHYLIDMSRRLEGLPRHSSTHAAG
                     VVISKKPIMEYVPLNANEGVITTQFPMTTLEELGLLKMDFLGLRTLTVIRNAVEQIEV
                     NHNIKIDIDKIDFNCKEVYDLISAGKTEGVFQLESSGMRSFMKELQPRTFEDIIAGIS
                     LYRPGPMDFIPKYIKGKNNPLAIEYATDALKPILAATYGCIVYQEQVMQIVRELSGYS
                     LGRSDLVRRAMAKKKAEVMAEERKNFIYGNGKDVPGCVNKGIPVAVAERIFDEMADFA
                     KYAFNKSHAAAYAVIAYQTAWLKAHYPIEFMAALMTSVMDNTDKLKEYIYACREMGIE
                     ILSPDINEGFASFSVSNGKIRYGLAAIKNVGRAMIAAIVEERNKNGKFLSMTDFFERM
                     DSGELNKRSIESLIKAGAFDSLGGKRSQYIAVFKQISDNINNRRKKAIEGQIDLFDFG
                     QEDITNEDILPDIEEYPAKVFLQMEKEVLGMYLSGHPLDEYEEELKKNSNVTSYDFIN
                     QESEERPVLVDGQVVQLGGMITEKSIKSTRSNQLMAFITLEDLYGSVEIIVFPNKYEE
                     YQKYLNEDEIIYVKGRVSLKEDEDAKLICEEIIPFDKMKDLRKQYNQKLYLKIPASEE
                     KFEVIEKVKEILKTSKGTIPVIIYIEATKETMKASKNLWINPKEDLIFKLKQVLGKEN
                     VLLK"
BASE COUNT         2400 a         1693 c         1045 g         2894 t
ORIGIN      
        1 gtgggtcaat ttctgtccgg cgctatgggt caattatacc ccggcggtga cacaagaaaa
       61 tcgttgatat aaatttatat aaatattttc ctttaaatct gtcatctatt ctatcccaca
      121 aaataaataa gcagcaaatt tccatacaag aaagaataaa agtttcatac catcgcattt
      181 attccaccac tctcacatca tttgttcttt ctataaaatg atttttatat gtgtttccta
      241 caaaagcagg tatatcgtaa tcatcgaaga aaacagtcca tgaattacgc tctcttttat
      301 caacttctcg tatgttatta atattgacaa tataagactt atgcgtttgc ataaaataat
      361 tttgaggcaa tatactgagc attttactca tattataatt gctaatttca tattcttttt
      421 tcttcgtatg gataatacag tttcttccct gagattctat aaataatatc tcatctacat
      481 agattcttaa gatacaactt ttaagtttaa atctgatttc ctcccgcttc tgaacatgtt
      541 ctttcttttg tctgataact gcacgggtca atttattaat cacatctaca atttctttat
      601 tttcataagg ctttacaata aaatcataac aatgtatttt tttaaatgca tctaataata
      661 aataaccatg ggttgtaata aaaaccatat gagcaaattc atattttggt acttttctaa
      721 ttttttctgc caacttaatc ccagatatat ccggaagctc aatatccatt aaaaataaat
      781 cgatatcatg attttttata atatttattg ccgtagaacc tttttctgct tcatatactt
      841 ttattccctc agtattagca ttaataattt gtaccaatct ttgtctttca taatcattat
      901 cttcaacgag taatacccgt atcatatgat cacccatgtg ttaaaatatt atataacata
      961 ttaccattaa tttcatttca atacaagaat tatgtagctt tttgtattaa aaaatcatat
     1021 ttataattta taagttattt ttctctaatc attttcaaaa aaaagcagag aaattctatg
     1081 aatttctctg ctttttactt ttgggaccct tattgttctt caaactcgtc tttacctaca
     1141 ccacaaactg gacataccca atcttccgga agatcttcaa atgcagttcc cggagcaatt
     1201 ccattatctg gatctccttc agcaggatca taaacataac cacaaactga acatacccat
     1261 tgactcataa tatttctccc ttcctcttta tatgaaaaac aactttaaaa gttgcttttc
     1321 ttataatata aagtagctta ttaaattttt cttatggtaa acaggttaaa tataacatgt
     1381 atagtatgca tattatacca tttgtttata ctaaatgcaa taattgttaa acacaaaggt
     1441 tattgaacat cttcctgtgc tccattatag actaatccat ttttactgtc cacagtgatt
     1501 aaggtgccat ctctgattaa gtccactgca tttttagcac caataattac tggaatatct
     1561 aatgctcttc caacgattgc agcatgagaa ttttcaccat gttccccttc ttcaacaata
     1621 atggctgaag cctttttaat gtatggaaga aagtcattat ccgtattact ggtgactaaa
     1681 atatctcctt ttttaaagta tttatccgct tcttctatta attttatgac tctggcagtt
     1741 cctgaaacgg tttttttacc aattcctttc cccttcacca atacatttcc cacaatgtgc
     1801 gcttttaaaa tattggttgt accactcacg ccaactggaa cacctgctgt aataactata
     1861 atatcaccat tcttagccaa acccgtttct acagattttt caaccgcttg attaaaaagc
     1921 tcatccgttg tatgcttaaa ttctgtttta acaggaatac agccccaaac taaatttaat
     1981 tgtctccagg ctttttcatc aacggtacat gctaatacag gcgcacaagg acggaattta
     2041 gaaaccattc ttgcagtacc cccagagcgt gttacggtaa caatgcatgc agcatttaag
     2101 tcggcagccg ttgtacaagt tgcatgactg atggcatctg ttatattgct ttgaatagac
     2161 gtatgaaact tagtcatatt cttaatgtaa tcaatagaac tctctgttct ttctgcaatg
     2221 gttgccatca tcaaaacact ttcaattgga taagcacctt ttgccgtttc tcctgaaagc
     2281 ataatagcat ccgtaccatc aaaaatagca ttagcaacgt cattggcttc tgcccttgtt
     2341 ggtcttgggt ttctaaccat tgaatccaac atttgagtag ccgtaataac cggcttgccc
     2401 atttggtttg ccttttttat taacatcttt tgaaccaggg gcacttcttc tatagggatt
     2461 tcaacaccca aatcccctct ggcgaccata atggcatcag ctacttccaa aatttcatcg
     2521 atattgtcta caccatctct attttcaatt ttagcaataa tatcaatatc gtttccgcca
     2581 ttttcttcaa gaattctacg tatttttata acatcgctgg cagaacggat aaaggaagct
     2641 gcaatataat caaagccttt ttttatccca aacttaatat cttctatgtc cttctctgtt
     2701 aaagaaggaa gattgacata gacatcagga atattaaccc cttttttaga actcaaaggt
     2761 ccaccattaa taacctcaca ttctacttct gtatcggtta aattcttaac cctaagttca
     2821 ataagaccat catcaataag aactctgctt cctcttctta aatcaaaggg taaattttta
     2881 taagtgacac taactcttgt ttcgtcccct tctacattct ctgtagtaag ggtaaaagtc
     2941 tgaccctgct gtaaaaatac cggctccttt acaaaatctt taatgcggat ttcaggacct
     3001 ttcgtatcca aaagcaaagg aatcggaata tttaattctt cccttgcttt aataagatta
     3061 tcaatgattt ttccatggga ttcataggtt ccatgagaaa aatttattct ggcagcattc
     3121 ataccgcttt ttatcaattt ttttatcgtg tccacatcac ttgtagccgg tcctaatgta
     3181 caaacaattt tagtttttct cattgttacc tccagttaag ctatctatga cataaaaaat
     3241 cctaaatttc atctataagg ataaaatctt tgaaacttca tacatttttt cattaatggt
     3301 tttcttcatt tgtaatgctt cattaatatc aaagtctaca tattttccat ctctgtacgc
     3361 tataacacgg ttgcttttac cttcacaaag aagttcaaca gcaatagcac ccatcattga
     3421 agcatgaagc ctgtccagtg cagaaggact acctcctctt tgcaaatgac ctaagataga
     3481 agcccttgtt tcaatacctg ttaattcttc tatttttttt gccaattgct ctgaaccacc
     3541 tactccttct gctgcaataa tcaaaaagtg ctttttccct ttctttttac tttccaaaat
     3601 ttctttaaca atttcttcat cagaaggtct ttctttttca ggaattaaaa ccatctcagc
     3661 cccattgctg atagcactcc aaagagcaat atatcctgaa tgtcttccca taacttctac
     3721 gatgctgcat ctttcatggg atgtagaagt atctctgatc ttatttattg cttccatagc
     3781 agtatttact gctgtatcaa aaccaatcgt ataatctgta caagcaatat ccagatcgat
     3841 ggtaccagga ataccaatcg tattaattcc taactttgat aacttttcag ctccttgtaa
     3901 tgaaccatct cctccaataa caacaaggcc ttccatacca aacactctac acatttctgc
     3961 tgctcttctt tgtccgtctt ccgtcataaa ctgagcacat cttgccgttt gaagaatggt
     4021 accccctctt tgaatcgtat ccgatacact ttttgtattc atttcaaata tatctccatt
     4081 gattaatccg ttatagcctt ttcttacgcc cattacttta atgccttttg aaatagctgt
     4141 tcttacaacg gatcttatag cagcattcat tcccggagca tctccgccac ttgtcaatac
     4201 accaatagta ctaatttttt tgctcattct tctacctcca ccatcttata ctctatcttt
     4261 atgcgacttc ttcaatcata ctagaagata cctacatttt cacatataca ttgtaatcaa
     4321 tttgtatttt ctttgcaata aaaagataag attttacgtt ttctataaat ttttatttta
     4381 aaagtacatt ttctttacct aaaacttgct ttaatttaaa aattaaatct tcttttggat
     4441 taatccataa attttttgat gctttcatgg tttcctttgt agcctctata tagatgataa
     4501 ccggaatcgt tcctttagaa gttttcagaa tttcttttac cttctcaatg acttcaaatt
     4561 tttcttcaga agcaggaatc tttaaataaa gcttttgatt atattgtttc cttaaatctt
     4621 tcatcttatc aaaaggaata atttcttcac agattaattt agcgtcttcg tcttctttta
     4681 aagaaaccct tcctttaaca tatatgattt catcttcatt gaggtacttt tgatattctt
     4741 catatttgtt aggaaaaaca ataatctcta cggaaccata taaatcttct aaagtgataa
     4801 atgccatcaa ttgattactt cttgttgatt taatgctttt ttcagtaatc attcctccta
     4861 attgtactac ttgaccatct actaaaacag gtctttcctc tgattcctga tttataaaat
     4921 cataacttgt aacattggaa tttttcttta attcttcttc atattcatcc aaaggatgcc
     4981 cgcttaaata catgcctaat acttcttttt ccatttgcaa aaaaactttt gcaggatatt
     5041 cttctatgtc aggaagaata tcctcatttg taatatcttc ttgtccaaaa tcaaataaat
     5101 caatctgtcc ttctatagct ttttttcgtc tgttattaat attgtcagaa atttgtttaa
     5161 aaactgcaat atactggctt ctctttcctc caagggaatc aaaagcccct gctttaatta
     5221 aactttcaat acttctttta tttaattcac cactgtccat tctttcaaaa aagtccgtca
     5281 tactaaggaa ctttccattc ttatttcttt cttctacaat cgcagcaatc attgcccggc
     5341 ctacattttt aatggcggct aatccatatc tgatttttcc atttgaaacg gaaaaagaag
     5401 caaaaccttc attgatatca ggtgaaagga tttctatgcc catttccctg caagcataaa
     5461 tgtattcttt cagcttgtcc gtattatcca tgacagaagt catcaaagca gccataaatt
     5521 caataggata atgggccttt aaccatgccg tctgatacgc aataaccgca taagccgctg
     5581 catgggattt gttaaaagca tatttggcaa aatcagccat ttcatcaaat attctttcag
     5641 caaccgctac aggtatgccc ttattcacac accccggaac atctttgcca ttaccataaa
     5701 tgaaattttt tctttcttca gccataacct ctgctttttt cttggccatg gctcttctaa
     5761 ccaaatcact tctgccaaga ctgtagcctg atagttctct tacaatttgc atcacttgtt
     5821 cctgataaac aatacagcca taagttgcag ctaagatagg ttttaatgca tctgtcgcat
     5881 attctatggc caggggatta ttttttcctt taatgtattt aggtataaaa tccatgggac
     5941 ctggacggta aagagaaatt cctgctatga tatcctcaaa agtcctgggt tgcagttctt
     6001 tcatgaaact cctcatgccg ctgctttcta actggaatac cccttctgtt tttcctgcag
     6061 aaatcaaatc gtagacttct ttacagttaa aatcaatttt atcaatatca attttaatat
     6121 tatgattcac ttcaatttgt tcaacggcat tgcgaatcac tgtcaaggtt cttaaaccta
     6181 aaaagtccat cttcagcaaa cccagttcct ctaaagtcgt catgggaaat tgtgtggtaa
     6241 taacgccttc attggcgttt aaaggaacat attccataat gggttttttg gatatcacga
     6301 cccctgcagc atgggttgat gaatgtcttg gaagtccttc taatctcctt gacatatcaa
     6361 tcagataatg gacttcttca tcattttcat acaaagcttt taattcaccg ttaatttcaa
     6421 gggccctgtc gatagttatt cctaattctg caggaatcat ttttgcaact ttgtccactt
     6481 ctgcataagg catattgatt gccctgccaa catcacggat gactgccctg gcagacatgg
     6541 ttccaaaagt gatgatttgt gcaacccggt cttctccata ttttcttatt acataatcaa
     6601 taacttcttg tcttctctca tagcaaaaat caatatcaat atcaggcatg ctgactcttt
     6661 caggatttag aaaacgttca aaaagcaatt gatattttat aggatctata ttcgtaatct
     6721 gaagacaata agcaaccata cttccagcag ctgagccccg tccgggtccc accataattc
     6781 cgttgtcttt agcatatttt ataaaatccc atacaattaa gaaataatca acatatccca
     6841 tccgttcaat cgtatctaac tcatattcaa gtcgtgcctt aagctcatca tcagcatcag
     6901 gatatctgtc attaaatcct tcgtagcaca attttcttaa atactctttt gctgtataac
     6961 cttctggtac atcatattca ggcagcttaa gctcatggaa ggtaaattct acgttacaac
     7021 gctgtgcaat ttcataagta ttccttaaag cttctttagc ataaggaaat ctatgataca
     7081 tttcctcagg ggatttgaga taaaattctc ccccagggta aaccatcctg tcctcatcat
     7141 tgatggtttt tgcggtttga atacaaagca gtatttcatg cgctttacta tcttctttat
     7201 aaatataatg aatatcattg gttgcaacca gagggatacc agtttcttta ctcatacgca
     7261 ccaggcattg gttaacttcc ccttgttctt taagaccatg gtcttgaagt tcaagataaa
     7321 aattaccttt ccctaatatt ctttcatatt gcaaagctac ttccactgct ttttcataag
     7381 actcttccct gatggccctg gcaacaggac cagccagaca agcacttagg cctattaaac
     7441 ctttggaatg cttttccagg agttctaaat ccactctggg tttatgataa aaaccttcta
     7501 taaagccata agaaaccagt ttaataaggt ttttatagcc ttccatattt tcagccaaaa
     7561 gaaccaaatg ataataattg gcatcctgtt tttctttatc aaaacggctt cttggagcga
     7621 catagatttc acagccaata atcggcttaa tcccttgttt tttagcttct ttatagaaat
     7681 caattacccc atacattgcc ccatggtcag taatggcaat actatccatt cccagttctt
     7741 ttgttcttgc aattaattct tcaatctttg cagaaccatc taaaagacta tattctgtat
     7801 gaacatgaag atgagtaaaa ggcattgaat tggtcaattg tttatccata tttggcttcc
     7861 ttccacactt acttaagctt ctgttataag cttattatag catactctca aaaatattat
     7921 atagatttat atcaatagtc ctttttgttt aaaatttatt gcataatttt tttaatgtag
     7981 taatatattc ttcatcggat attttaaata ttgaggtaaa aattatttat aa
//
LOCUS       DAOSUD010000085         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4876_length_7565_cov_19.710652, whole genome shotgun sequence.
ACCESSION   DAOSUD010000085 DAOSUD010000000
VERSION     DAOSUD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_7565_cov_19.710652"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLG49_09270"
     CDS             complement(<1..453)
                     /locus_tag="PLG49_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOQ17534.1"
                     /translation="MAKYRCSVCGYIHEGEMTEDFKCPRCKKPASFFVKIEEETKRKN
                     PYAGTKTEKNLMEAFAGESQARNKYTYFAQIAQREGYDQIAELFLKTARNEQEHARIW
                     FEELGGLGNTAENLLKAAEGENYEWTDMYDRFAKDAEEEGFPELAEKFR"
     gene            complement(518..907)
                     /locus_tag="PLG49_09275"
     CDS             complement(518..907)
                     /locus_tag="PLG49_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOQ17535.1"
                     /translation="MKKEEKSFYLCNHCGNLVGLINNAGVPLVCCGEKMQELIPNTVE
                     ASQEKHIPVIKQEGNKVILEIGSAPHPMVDEHYITWVYIQTKKGGQRKELYPGQEPYV
                     EFALTEDDEIEIAYAYCNLHGLWKASL"
     gene            complement(1104..2437)
                     /locus_tag="PLG49_09280"
                     /pseudo
     CDS             complement(1104..2437)
                     /locus_tag="PLG49_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(2683..4017)
                     /locus_tag="PLG49_09285"
     CDS             complement(2683..4017)
                     /locus_tag="PLG49_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ17536.1"
                     /translation="MEKSIFKNFSRYVSLNILGMVSISCYILTDTYFISKALGHQGLA
                     ALDLSTPMYSFIYAVALMLAIGGSTRYSILKAKKDTIRANRVYSICINVGLITGILFA
                     MIGIFFSNSLGVLLGADIEILPMTEIYLRTILCFSPFFILNDIILSFVRNDNNPNLPM
                     IAILSGSFFNIVLDYIFIFYFDMGMFGAAFATGLAPIISLVVLASYFIRAKNHFKYIP
                     FKFNWSYLKDISTLGLSAFVNEISAAIVIITFNLVILNLEGNYGLASYAIIANIDLVV
                     FAVFNGLAEGIQPIISKSHGLNDDFVLKKTLKYALLTSLGLALLIYFTLNFNAESIIR
                     AFNEEENIKIAIIGKAGLKIYFIGYFFAGINITLTMFLTATEKARLAFLLSVLRGCIV
                     IVPMVLVLSCLWKMTGVWLSFVLAELFVTVIAIFIMGVKSRQIIKLKFENNS"
     gene            complement(4132..4830)
                     /locus_tag="PLG49_09290"
     CDS             complement(4132..4830)
                     /locus_tag="PLG49_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOQ17537.1"
                     /translation="MKSSTVSMVTNFFIWIKKLFIIVLIIILITVIVISLINYYMKRS
                     VKDRIFESAEDIDFEADCILVLGAGVKNERPSHMLEDRLKQGIKLYELGASDRLLMSG
                     DHGRKEYDEVNVMKKFAIDAGVPSEHIFMDHAGFSTYESLYRARDIFEAKKVIIVTQE
                     YHLYRALYISDKLGLEACGVSSDLRPYYGQKYREFREYFARAKDFIWTIFKPEPTYLG
                     ETIPISGNGDLTND"
     gene            5118..5840
                     /locus_tag="PLG49_09295"
     CDS             5118..5840
                     /locus_tag="PLG49_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOQ17538.1"
                     /translation="MFKRPIYKTNNPYIVFAARGDVTGDGIKDYIYLTGISKEDSPFV
                     QRITLVIKDGRTASTVTVPLKTDAGYSPRIFLGDFTGNGVYDILISITSGGSGGIIYY
                     YIYSALNNIPKLLFDYEAFNEKYQYQVIYKDNYKVELINTTRKIRYIIDISYRDKEYL
                     DEIYNPDGSLKAPVEGFINPLSGLYPIDFDSNDVYELLGYQRISGRYAADALGYLQTS
                     LEWDKNQFIVREQSVNIFGSDY"
     gene            complement(5906..6139)
                     /locus_tag="PLG49_09300"
     CDS             complement(5906..6139)
                     /locus_tag="PLG49_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HOQ17539.1"
                     /translation="MSHKSKITKVKKNSEGDITDVMLDDGSVYPIDEAIEMVKDDLIE
                     GVNVGKAKNGREFLRADPNGDLTDNLDNLSTFE"
     gene            6367..7263
                     /locus_tag="PLG49_09305"
     CDS             6367..7263
                     /locus_tag="PLG49_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOQ17540.1"
                     /translation="MLSKQEYIRLSIENNLFFQRIIKEHLFFIETNLPTVESVNISRA
                     DMLKRNFEQLLAETTFYAKWFVSEQAIKSNEFVTPYTLRAEELSAKLTGATLNTEITK
                     AELSLFNVPSRCTTEHLEAIINDLNKRSIILVKQVIDFQNKLLFLLSECQLFITLYNE
                     MLEHLNHESEYYLRLLESLQNKSLSKKQLCRELNFWNHIMENHAQFITGMLDPTEINL
                     KEIAETIAKKFETLIKICLQTPEKEILNMSLEAVTEIRNFKEEATKQILSCKIKSIIL
                     PLLADHVLREANHYIRLLNTIK"
     gene            7377..7565
                     /locus_tag="PLG49_09310"
     CDS             7377..7565
                     /locus_tag="PLG49_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOQ17541.1"
                     /translation="MTCNNNVQCNCTYTSCSRHGKCCECIAYHRRCNEVPGCLFSKAG
                     ERTYDRSIENLYNDYKNR"
BASE COUNT         2717 a         1373 c         1015 g         2460 t
ORIGIN      
        1 tctaaacttt tctgctaact ctggaaaacc ttcttcttct gcatcttttg caaatcgatc
       61 atacatatcg gtccattcat aattttcgcc ttcagcagct tttagaagat tttctgcagt
      121 atttcctaag ccacctaatt cttcaaacca aatacgggca tgttcttgtt catttctggc
      181 agtttttaag aaaagttcag caatttgatc ataaccttcc ctttgagcga tttgtgcaaa
      241 atatgtatat ttatttcttg cctgactttc tcctgcaaaa gcttccatga gatttttttc
      301 tgtttttgta cctgcataag ggttttttct ctttgtttct tcttcaattt ttacaaagaa
      361 tgaagcaggc ttcttacatc ttggacattt aaaatcttca gtcatctctc cctcatggat
      421 gtatccacat acactgcagc gatattttgc cataataatt cctcctatta ttttttaatc
      481 ttaatagatt taaaatttta tattcttatc tgtattttta aaggctagct ttccaaagtc
      541 catgtaaatt acaatatgca taagcaattt ctatttcatc atcttctgtt agagcaaatt
      601 ctacataagg ttcctgacca ggataaagct cctttctctg tccacctttc tttgtttgaa
      661 tatataccca ggtaatataa tgttcatcca ccataggatg gggtgctgaa ccaatttcta
      721 aaatgacctt atttccttct tgcttaatta ctggaatatg tttttcctgg gaagcctcca
      781 cggtgtttgg aattaactcc tgcatttttt caccacaaca cactaaagga acccccgcat
      841 tgttaattaa gccaaccaaa tttccgcaat gattacagag ataaaaactt ttttcttctt
      901 tcttcattaa aaatcactcc tttttataaa aatatttaaa gagcttaatt tattatagaa
      961 aaattattat tttaattcaa ttattagtta ataatttttc tacactagca atattatact
     1021 tttatacttt tcttttttca agaaaagttc aagaaattat tataattttt atcttaatca
     1081 gatgtaatag ataaatataa gctttaacgg ctattctcaa attgcagttt tattgctaaa
     1141 tcacttttca aaagccatat aaagcttaca actatggtta caaaaaattc tgtgaaaaca
     1201 aaggaaagcc atacccctgt catcttccac caactgctta ataataaaac cataggtacg
     1261 atgataagac aacctcttaa aatagaaata ataaaagcat tcctggcttg ttctgttgcc
     1321 gttaaataca tcgccataca aatatttata ccggcaaaga aaaaacccat aaaatagata
     1381 ttaagtcctc tttttgcaat ctggcttatt tgcggatttt gttcattatt aaagagatga
     1441 ataatgtttt tactgttata aaaaacgcca agataaacta acactgccaa aactattgca
     1501 gtaacgatgg catacttaag tatttttttc aatagcactt tatcgtttaa tccataagcc
     1561 tgactaataa tcggttgtat tccttgcgca agaccagtaa atatagcaag acctacaaga
     1621 gcaatatttg cgacaattcc ataagaagcc agtccaatgt ttccctcaag atttaaaata
     1681 actaagttaa aaataataag gactacagca gatgaaattt catttataaa agcagacaaa
     1741 cctaatgctg aaatatctat aagacaggaa atttgaaata tataacgaat atagtgaaaa
     1801 ccattgtttt tccttataaa atgaattgac aatactccta aactaattac aggcgctaaa
     1861 caggttgcaa aagcagcacc aaacatgccc attttaagtg gaaacataaa aatataatct
     1921 aataaaatat tcgataaact tcctgtaagc atagcaatca ttgataattt tggattatta
     1981 tcatttcgta caaaagaaat cataatgtta ttaaggagaa aaaaaactgc aaaaaagagt
     2041 attgttttta aatagctttc cgtcatgcct aaagtttcat gattagctcc taaaagacat
     2101 cccaaaggtc ttgaaaagaa aaaccaatga ttgcaaaaat catgccaata atgaaagagg
     2161 atttaaaaca aattgtaaat actttgtccg cttcctcatc ttcttttttt gattttaata
     2221 tgctatattt gcttgcacca ccaatagcca gcatcaaccc aagaccatga atgacactgt
     2281 atactgaaat tgaaagatta agcgctgcaa tgccagaagg tcctaatgct cttgaaataa
     2341 aaaacgtatc tgccaggata taacacgaaa tacctatcat tcctaaaata cttaaactta
     2401 tatatctaat aaactgttta agaccagatt catccatcct tctctctcct tatcaatttt
     2461 aagcttgcca aatgaaattt ttcaaaaacg tccatatatg taaaaaagtg ttcaaaaaca
     2521 ccccttctaa ggcctaacgg actgtttaga acacttatta acctttaccc ggcggcaaag
     2581 taaaaattct aatattcgtt taaacaaagc catgataaga tatttcgaaa agattgtcaa
     2641 gaaaacctgt taatgtggtt tacaaaggaa attaacctta atctaagagt tattctcaaa
     2701 tttcaatttt attatttgcc tgctctttac tcccataata aaaattgcaa tgacagtaac
     2761 aaataattct gccaaaacaa aggaaagcca taccccagtc attttccaca ggcagcttaa
     2821 tactaaaacc ataggtacga tgacaataca gccccgtaaa acggaaagta aaaaggccag
     2881 tcttgctttt tcagtagctg ttaaaaacat tgtcaaagta atattgatac cagcaaaaaa
     2941 ataacctata aagtatatct taaggccagc ttttccaatg atggctattt ttatattttc
     3001 ttcttcatta aaagcacgaa taatgctttc ggcattaaaa tttaaagtaa aatatattaa
     3061 aagtgccaaa cctaaggaag ttaacaaagc atatttcagg gtttttttca atacaaaatc
     3121 atcatttaag ccatggcttt tactaataat gggctgtata ccttctgcaa ggccattaaa
     3181 tacagcaaaa acaacaagat ctatattggc aatgattgca taagaagcaa gcccataatt
     3241 accctcgaga tttaaaatga ctaagttaaa agtgataata acaatagcag ctgatatttc
     3301 attaacaaaa gcagacagac ctaaagtgga tatatcttta agataagacc agttaaattt
     3361 aaatggaata tatttaaaat gattctttgc tcttataaaa taacttgcta atacaactaa
     3421 acttattatg ggcgctaaac ccgttgcaaa agcagcgccg aacataccca tatcaaaata
     3481 aaagataaaa atatagtcta aaacaatatt aaaaaagcta cccgataata tcgcaatcat
     3541 aggtaaatta ggattattat cattacggac aaaagaaaga ataatatcat ttaaaataaa
     3601 aaaaggagaa aaacaaagga ttgtcctcaa atatatttct gtcatgggta atatttctat
     3661 gtcagcacct aaaaggacgc ctaatgaatt tgaaaaaaat ataccgatca ttgcaaaaag
     3721 aatacctgta atcaagccaa cattgataca aatactatag accctgttcg cccggatcgt
     3781 atcctttttt gcttttagta tgctatacct tgtagagccg cctatggcaa gcattaaagc
     3841 aacagcataa ataaaactat acatgggtgt agaaagatca agggctgcaa gcccctgatg
     3901 acctaatgct tttgatatga aatatgtatc tgttaatata taacatgaaa tactcaccat
     3961 tcctaatatg tttaaactaa catatctgga aaaattcttg aatatagatt tctccatata
     4021 ttcctcccaa aatatgtcca ctatttcctg tcccaaaacg agtcggttaa atttttaaaa
     4081 gaacacatta aatttattca tatttttctt aaaccattga atttctaaat attaatcatt
     4141 ggttaaatcc ccattaccgc taataggtat agtttctccc agataggtgg gctctggttt
     4201 aaaaatggtc caaataaaat cttttgctct ggcaaaatac tcacgaaatt cccggtattt
     4261 ttgaccataa tatggcctga ggtcagaaga cactccacag gcttcaagtc ctaacttgtc
     4321 cgaaatatat aaagcacggt acaaatggta ttcttgcgtt acaatgataa ctttttttgc
     4381 ttcaaatata tctcttgccc tgtacaaact ttcataggtt gaaaaacctg catgatccat
     4441 aaaaatatgc tctgaaggta ccccggcatc aatagcaaat ttcttcatca cattcacttc
     4501 atcatattct tttctgccat gatctccact cattaataat ctatcggatg cacccaactc
     4561 ataaagttta atcccctgct ttaatctgtc ttctaacata tgacttggtc tttcattttt
     4621 tacgcctgct ccaaggacca aaatgcagtc tgcttcaaaa tcaatatctt ctgctgactc
     4681 aaaaatcctg tcttttacag atctcttcat ataataatta attaacgata taacaataac
     4741 cgttatcaaa ataatgatta agacaataat aaatagcttt tttatccaaa taaaaaaatt
     4801 cgtcaccata gatactgtac tgctcttcat cccaattctc ctgtccactc tattttaaat
     4861 acctaaaaaa atgtataaat ttaagaacaa tacttaaaaa atttttacct atctatttct
     4921 actctatagc ttttctacat attaatatta tacgtaattt tttagcagat tccagtaaat
     4981 tattaaaaat aatccgtttt ttgtcgtaaa atttgttatt accacactta ttaaaaaatt
     5041 ttttaataaa gattctgtca ccattttggt ctttgcttca taaaatatta ataagacata
     5101 ttagcggggg aatgttcatg tttaagcgcc ctatatataa gacaaataac ccatatatcg
     5161 tttttgcagc acgcggagat gtgacaggtg acggtataaa agattatatt tatctgacag
     5221 gcataagtaa agaagatagc ccctttgtgc agcgtattac cttagtcatt aaagatggca
     5281 gaacagcttc tactgttacg gttcctctta aaacagacgc tggttacagc ccgagaattt
     5341 ttcttggtga ctttaccgga aatggcgtat atgatatttt aatatccata acatcaggtg
     5401 gtagtggagg tattatttac tattatatct actctgctct taacaatatc cccaaattat
     5461 tatttgacta tgaagctttt aatgaaaaat atcagtatca agtcatatac aaagacaatt
     5521 ataaagtaga gctaataaac actacaagaa aaatcagata tatcatcgat atttcctata
     5581 gagacaaaga atatctggat gaaatatata atccggatgg cagtctgaaa gctcccgtag
     5641 aaggatttat caatccctta agcggtcttt atcccattga ttttgattcc aatgatgttt
     5701 atgaactgtt aggataccaa agaatttccg gaagatatgc agccgatgcc cttggatatc
     5761 ttcagacatc tttggaatgg gataaaaatc aatttattgt tcgggagcaa tctgtcaata
     5821 tttttggttc agattattag aaaaaagcac tttaatacaa tacccgtact tctaaatacc
     5881 agaaagtacg ggtatttatt tttgtttatt caaatgttga gaggttatct aaattatctg
     5941 ttagatcccc attagggtca gcccttaaaa attcacggcc attcttagct tttcctacat
     6001 taacaccttc aattaaatca tcttttacca tttcaattgc ttcgtcaata ggataaacac
     6061 tgccgtcatc cagcattaca tcggtaatat ccccttctga atttttcttc acttttgtaa
     6121 tctttgactt atggctcata aaaaacctcc ttttaaataa tttcatagat ttggaaaatc
     6181 tcacaatcct attatttctt agaatcaaaa aattattcct ttatagtcat atttaataac
     6241 aagaaaattc caatcatata aataataaaa ggtcatttta tgtatttttg aaaagtttca
     6301 taaagtcaca ttttattttt cttaaaatat tatgtattaa gtaatgtctt gaaaggagat
     6361 tttttaatgc tgtccaaaca agaatatatc cgactttcca tagaaaataa tttatttttt
     6421 caaagaatca ttaaagaaca tttatttttt atcgaaacaa atttaccaac agtggaaagc
     6481 gttaatattt caagagcaga catgttaaaa agaaattttg aacaacttct tgctgaaaca
     6541 actttttatg caaaatggtt tgtttcagaa caagcaatta aatccaatga gtttgtaacc
     6601 ccttatacct taagagctga agagctaagc gcaaaactta cgggagccac gcttaatact
     6661 gagattacaa aagcagaatt gtcactgttt aatgttccga gccgctgcac aacagaacat
     6721 ttagaagcaa tcattaatga cttaaataaa cgttccatca ttttagtaaa gcaagtcata
     6781 gactttcaaa acaaattatt attcctgctt tcagaatgtc agttatttat caccttatat
     6841 aacgaaatgc tggaacatct taaccatgaa tccgaatatt atttgcgact attagagtcc
     6901 ttacaaaaca aaagcctgtc taaaaagcag ttatgcaggg aacttaattt ctggaatcat
     6961 attatggaaa accatgcgca attcatcact ggcatgttgg atccaacaga aataaatctt
     7021 aaagaaattg cagaaacaat tgctaaaaaa ttcgaaacgc ttataaaaat atgccttcaa
     7081 acacctgaga aagaaatcct taatatgagc cttgaagcag taacagaaat cagaaacttc
     7141 aaagaagaag caaccaaaca aatactgtct tgcaaaataa aatcaattat tcttccgctg
     7201 cttgcagacc atgtacttag ggaagccaat cactatatca gacttttaaa tacaatcaaa
     7261 taagcatttt aatatagtct tcctcctata caatccatga aatcctatac tatatattta
     7321 caatagtccc tatggatgtt tataatataa tcagaatgaa gtgtaggagg gataaaatga
     7381 cctgtaataa taatgttcaa tgcaactgca cttatacatc ttgttcaaga cacggaaaat
     7441 gctgtgaatg catcgcatat catagaagat gcaatgaagt acctggttgt ttgttttcaa
     7501 aagctgggga aagaacctat gaccgttcca tagaaaacct gtataatgat tataaaaaca
     7561 gataa
//
LOCUS       DAOSUD010000086         7531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4890_length_7531_cov_22.841359, whole genome shotgun sequence.
ACCESSION   DAOSUD010000086 DAOSUD010000000
VERSION     DAOSUD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4890_length_7531_cov_22.841359"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..678
                     /locus_tag="PLG49_09315"
     CDS             406..678
                     /locus_tag="PLG49_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17542.1"
                     /translation="MGKLSPHFKEELNKKEINLIILLSLIGFFMYLFINLLFPFANTI
                     SVVFLAITVGLTIYSLINKNYHLLYYALVSIILSIFLALWTHHFFS"
     gene            complement(714..1070)
                     /gene="rplT"
                     /locus_tag="PLG49_09320"
     CDS             complement(714..1070)
                     /gene="rplT"
                     /locus_tag="PLG49_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ17543.1"
                     /translation="MARVKGGLSAKKRHKRVLKLAKGYYGARSKQYRTAKQSVMRALS
                     SAYAGRKQRKRQFRQLWITRINAAARLNGLSYSKFMNGLKLAGVNINRKVLADMAVTD
                     ADGFAQLVEIAKTNLK"
     gene            complement(1102..1299)
                     /gene="rpmI"
                     /locus_tag="PLG49_09325"
     CDS             complement(1102..1299)
                     /gene="rpmI"
                     /locus_tag="PLG49_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ17544.1"
                     /translation="MPKLKTHRGAAKRFKLTGTGKIKRAKAYKSHILTKKTAKRKRNL
                     RQPGLVDKSNVKQMKKLLPYL"
     gene            complement(1344..1856)
                     /gene="infC"
                     /locus_tag="PLG49_09330"
     CDS             complement(1344..1856)
                     /gene="infC"
                     /locus_tag="PLG49_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ17545.1"
                     /translation="MSDLLINEQIRDKEVRLIDVDGKQLGIVSIQEAQAVANERNLDL
                     VKIAPKANPPVCRIMDYGKYKFDMAKKEKEAKKKQKVINVKEVRLSPNIDKHDLETKI
                     KSAIKFLKGGDKVKVTVRFKGREMAHLEKGKELLDNFAKAIEEYGQVEKQAKLEGKNN
                     LVIIVTPLSM"
     gene            complement(2081..4018)
                     /gene="thrS"
                     /locus_tag="PLG49_09335"
     CDS             complement(2081..4018)
                     /gene="thrS"
                     /locus_tag="PLG49_09335"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOQ17546.1"
                     /translation="MVKIKLKDGSVKAYESGITVLEVAKDLSKGLARVACAGMINDEL
                     VDLRTPIHEDCELKILTFDDEGGKHAFHHTSSHILAQAVKRLFPQAKLAIGPAIEDGF
                     YYDIDTDKPFSPEDLEKIEEEMKKIVKEDLEIERFELPREEAIQLMQEKGENYKVELI
                     NDLPEDAVISFYKQGEFIDLCAGPHLMSTKPVKAFKLLSLAGAYWRGNEKNKMLTRIY
                     GTSFTKKSDLEAYLHKLEEAKKRDHRKLGKELELFTLMEEGPGFPFFLPKGMELRNTL
                     IDFWRQKHREAGYQEISTPIILNQELWHRSGHWDHYKDNMYVTKIDDMDYAVKPMNCP
                     GGMLVYKTKMHSYRDLPIRLAELGLVHRHELSGALHGLMRVCNFTQDDAHIFMLPEQI
                     KEEIKRIIDLIDDFYNIFGFKYHVELSTRPEDSMGSDEDWDRAINALKEVLEEKGYDY
                     VINEGDGAFYGPKIDFHLEDCLGRTWQCGTIQLDFQMPERFDLVYIGQDGEKHRPVMV
                     HRVVFGSIERFIGILIEHYAGAFPTWLAPVQVKVLPISEKYHDYAQKVQSLLEKEGLK
                     VEGDYRAEKIGYKIREARLQRVPYMLIIGQKEEEENTVAVRSREKGDEGSQDLDSFIS
                     RIKEEIETKKYTVQSLDNDEK"
     misc_binding    complement(4124..4329)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4402..5298)
                     /locus_tag="PLG49_09340"
     CDS             complement(4402..5298)
                     /locus_tag="PLG49_09340"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HOQ17547.1"
                     /translation="MVVLTIGAIKYWDKIKEQIDIEFEKIDQKELIPECRFHKKGDIH
                     FLIFEIDEKNLLDDNGSFYTEGLKYYVSSAITNVILNYTRFDIITEILENKYLRYMSE
                     NRKILDTIKEFFTENDNNDSPHVFNYSYRKEIMNALIDFFEDNNEFIYEGFVQFRMKK
                     YREILERAIDFIVKEYETQKEYKEFLRLLKYFVDVQEMKEESVHIVGIKNGQFKLYDK
                     DKKDITNRCKEEFIQDFNHKDINYDDFLVSALITIAPKQIIIHNYQELENRPIFETII
                     NIFQGKVTICDECDFCINSIEK"
     gene            complement(5475..6347)
                     /gene="hslO"
                     /locus_tag="PLG49_09345"
     CDS             complement(5475..6347)
                     /gene="hslO"
                     /locus_tag="PLG49_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOQ17548.1"
                     /translation="MNDYIIRATDKKKQVRAFAAITTNLVEEGRRIHNTSPVVSAALG
                     RLLTAAGMMGYMLKGEKDIITLQIRGDGPIKGLVVTSDSKGNVRGYPYNSLVDLPLNS
                     IGKLDVKTAIGNGYLTVIKDLGLKEPYIGQTKLVSGEIAEDLTYYFYNSEQIPSAVAL
                     GVLVDRDWSIKKAGGFIIQLMPEADEETISTLESNLKKIHSVTSLLDEGKTPEELLEI
                     LLGEIEINDKLPVQYHCNCSREKIKKALISIGLKDLKEMLEEDKQGEVKCHFCNTAYL
                     FNEEDLKEIIKELS"
     gene            complement(6352..7119)
                     /locus_tag="PLG49_09350"
     CDS             complement(6352..7119)
                     /locus_tag="PLG49_09350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ17549.1"
                     /translation="MEPYKSFALVYDLFMQEVPYEEWVKYIEKIWERFNCSPELMAEL
                     GCGTGNLTTLFAKKGIDMIGIDNSPDMLTIAKEKALSEHLDILYLLQDMREFELYGTV
                     DCIISLCDSLNYILDEEDLLQTFKWVNNYLNPGGLFIFDMNTEYKYKYILGDNIFADS
                     YDEAAFIWNNFYDEDKALNEYHLTLFIRQEDTNLYERYEEIHYEKMYSVSQLIGLIEK
                     AGLEFLAVYDAYSFDPPKENSERLFFVAREKGKTDRR"
     gene            complement(7206..7373)
                     /locus_tag="PLG49_09355"
     CDS             complement(7206..7373)
                     /locus_tag="PLG49_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOQ17550.1"
                     /translation="MTEDEKLKYEIASELGLLEKVEKEGWKSLTAKETGRIGGLMTKR
                     KKEITKQQTQN"
BASE COUNT         2264 a         1510 c          934 g         2823 t
ORIGIN      
        1 ttcagttttc aaggatcaaa ttttgaatgc tgcgctaatg agcgacagcg aaatacatct
       61 tatcacagaa aaatatttgt gtcaacattt tttgagcttg aattttttgt aattaataga
      121 tattttttct ttgagctaaa aaaagcttat attatcagct ttattaaaca caatagatgc
      181 tattattatt ttgtttaaaa aaatattttt tatgcatatt gcactttgtt attcataact
      241 tgcttattta tttcatagat atattataaa attaaatcat ttgcttttat tacaatatct
      301 tgcactattc aagcttctta cttttaactt cttaacttca acgaattgtt attattttta
      361 acttccagtc ttcaacttct aacttctttt tttaaggagc tgattatggg taaattatct
      421 cctcatttta aagaagaatt aaataaaaaa gaaattaatc ttatcattct tctgtctctt
      481 atcggctttt ttatgtattt atttattaat ttgcttttcc cttttgccaa taccatatct
      541 gtcgtttttt tagcaattac ggtaggctta actatttaca gtttgattaa taaaaattat
      601 catctgcttt attatgcgct tgtttcaatt atcttatcga tttttctggc cctttggacc
      661 caccactttt tttcataaaa aaataagcac attcctgtgc ttattttttt atattatttt
      721 aaatttgttt ttgcaatttc tactaattgt gcaaatccgt cagcatcagt cactgccata
      781 tctgctaaaa ctttgcggtt gatattaaca cctgcaagct taagtccatt cataaattta
      841 ctgtaagaaa gaccatttaa tctggctgca gcattaattc tggtaatcca aagttgacga
      901 aattgtcttt ttctttgttt tctgccagca taagcagaac tcaaagctct cattacagat
      961 tgtttagcag ttctatattg tttggatctt gcaccataat aacctttagc taactttaga
     1021 actcttttat gtcttttttt ggcactcagt ccgcctttta ctcttgccat ctttaaccct
     1081 cctgttcttt ctttctaatt gctataaata aggtaaaagt tttttcattt gtttaacatt
     1141 tgatttatca actaaacctg gttgtctgag gtttcttttt cttttagctg ttttcttggt
     1201 taaaatatga cttttataag ccttagctct tttaatctta cctgttccag taagcttaaa
     1261 acgttttgct gctcctctat gggtttttaa tttaggcatt tatgtttcct cctttatacg
     1321 aaatataaaa tatataatag acgttacatc gaaagcggtg taacgattat taccaaatta
     1381 ttctttcctt ccaattttgc ttgtttttcc acctgtccat attcttctat ggcttttgca
     1441 aagttatcca acaattcttt tcctttctct aaatgagcca tttccctgcc tttaaatctc
     1501 accgtaacct taactttatc tccgcctttt aaaaatttaa tggcactttt aatttttgtc
     1561 tccaaatcat gcttatcaat attgggagac aaacgaactt cttttacatt gattactttc
     1621 tgctttttct tggcttcctt ctctttcttt gccatatcaa atttgtattt accataatcc
     1681 ataatgcggc atacaggtgg atttgccttt ggggcaattt ttaccaaatc gagattcctt
     1741 tcattggcta cagcttgtgc ttcctgaatt gaaacaatcc ctagttgctt tccgtcaaca
     1801 tcaataagcc tcacttcttt gtcacgaatt tgctcgttaa ttaataaatc gctaatagtc
     1861 tagcacctcc taaaaatatt tataagattt tgtattaaaa aaagcggata gaaacctatc
     1921 cgcttacaca ctcacaaaaa gagtcttata tcataagcca taaaagactt aaaatactct
     1981 ttaaatgaaa tcataaaccc tgtcgactta taaagcgcaa ggtgagaaac ggacgtttct
     2041 gcttaaaaga caaatattca caacaatagt taatatatca ttatttttca tcattgtcaa
     2101 gactttgtac agtatatttt ttcgtttcaa tttcttcttt aatcctacta ataaagctgt
     2161 ccaaatcctg acttccttca tcgccttttt cgcggcttct tacagcaact gtgttttcct
     2221 cttcttcttt ttgaccaata atcagcatat aaggtactct ctgcaatctt gcttcacgaa
     2281 tcttatagcc gattttttca gctctatagt caccttctac ttttaatcct tctttttcta
     2341 ataaggactg cactttttga gcataatcat gatatttttc agaaataggt aatactttta
     2401 cctgtacagg ggcaagccat gtggggaaag cacctgcata atgttcaatt aaaataccaa
     2461 taaacctttc aatgcttcca aaaacaactc tgtgaaccat aacagggcgg tgtttctctc
     2521 catcctgacc gatataaact aaatcaaaac gttctggcat ctggaagtca agctgaatgg
     2581 ttccacactg ccaggttctg cctaagcaat cctctaaatg aaaatcaatt ttgggtccat
     2641 aaaaagcacc atcaccctca ttgataacat aatcataacc tttttcttct aatacttctt
     2701 ttaaggcatt gattgccctg tcccaatctt catcactgcc catactgtct tcaggtcttg
     2761 ttgaaagctc aacatgatat ttaaatccaa agatattata gaaatcatca atcaagtcta
     2821 tgattcgttt tatttcttct ttaatttgct ctggcagcat aaaaatgtgc gcatcgtctt
     2881 gagtaaaatt acaaactctc ataagaccat gaagggcacc tgataattca tggcgatgga
     2941 ccagacctaa ttctgccaaa cggattggta aatccctgta ggaatgcatt ttggtcttgt
     3001 aaaccagcat accgcctgga caattcatcg gttttacagc ataatccata tcatcaattt
     3061 tggtaacata catattgtct ttataatgat cccaatgacc tgaacgatgc cataattcct
     3121 gatttaaaat aataggcgta ctaatttcct gataacctgc ttccctatgt ttttgtctcc
     3181 aaaaatcaat taaagtattt ctgagctcca tgcccttagg caggaaaaat ggaaaacctg
     3241 gaccctcttc cattaaagta aaaagttcta attcctttcc aagctttcta tggtctcttt
     3301 ttttggcttc ttctaacttg tgaagataag cctccaaatc agactttttg gtaaaggaag
     3361 tcccataaat tcttgtgagc atcttattct tttcattgcc tctccaataa gcaccggcta
     3421 aggacaataa tttgaaagcc ttaacaggtt tggtagacat aaggtgagga cccgcacaca
     3481 aatcaataaa ttctccttgt ttatagaaag agataactgc atcttcagga agatcattga
     3541 tcaattcaac tttataattt tctccttttt cctgcatcaa ttgaatagct tcttctcttg
     3601 gcaattcaaa tctctctatt tctaaatctt ctttaacaat ctttttcatt tcttcttcaa
     3661 ttttttctaa atcttccggg ctaaagggct tgtccgtatc aatatcataa taaaagccgt
     3721 cttcaatagc tggaccaatt gctaatttag cctgtggaaa taatcttttt actgcctgag
     3781 ccaaaatgtg agaactggta tgatgaaaag catgtttacc tccttcatca tcaaaggtta
     3841 aaattttcag ttcacagtct tcatgaatag gtgtccttaa atccacaagc tcatcattaa
     3901 tcattcctgc acaagccact cttgccagac ctttacttaa atctttagca acttccagga
     3961 ctgttatacc tgattcatac gctttcaccg aaccatcttt taactttatc ttaaccattt
     4021 ttcttctcct ttcttctaac cttaaggaaa taaattttgc tattatgcaa cttaaacgat
     4081 attatattta ctttcttaca taaaaaaact cgtctctttt atgcaaaggg acgagaggat
     4141 agctttctcc cgcggttcca ccctaattgt ttcaaggaaa ccactttatt atgattataa
     4201 cggcatcacc gtacctgatt agggtcgctc cgaggtggtc ttcatcaaac taagctgaag
     4261 atactcacag cttttgtacc ttctctctga aaacttatgt ctgactactc ttctcatcat
     4321 cgcattcaaa tataattatt atataaaatt ataacatgta tgaattcctt gtcaagaaat
     4381 aataaataaa ggtcatttca tttatttttc tatagaatta atacaaaaat cacactcatc
     4441 acaaatagta actttacctt gaaagatatt aataatggtt tcaaaaatcg gtctgttttc
     4501 taattcctga taattatgaa taataatttg ttttggtgca atggtaatca aagcactgac
     4561 taaaaagtca tcataattaa tgtctttatg attaaagtct tgtataaact cttctttaca
     4621 gcggttggta atgtcttttt tgtccttgtc atataattta aactgaccat tttttattcc
     4681 tacgatatgg accgattctt ctttcatttc ctgaacatcc acaaaatatt taaggagtct
     4741 taaaaattct ttatattctt tttgggtttc atactccttt acaataaaat caatagctct
     4801 ttctaaaatt tccctgtatt ttttcatcct gaactgaaca aagccttcat aaataaattc
     4861 attattatct tcaaaaaagt ctatcaaagc attcataatt tcttttctgt aagaataatt
     4921 aaaaacatgg ggactgtcat tattgtcgtt ttcagtgaaa aactctttaa tggtatctaa
     4981 tattttccta ttttcagaca tataacgaag atatttattt tctaaaatct cagtaattat
     5041 atcaaagcgg gtataattta atattacatt tgtaattgca ctgcttacat aatacttaag
     5101 accttctgta taaaatgaac cattatcatc taacagattc ttttcgtcaa tctcaaagat
     5161 taaaaaatga atatcgcctt ttttatgaaa acgacactct ggaatcaatt ccttctggtc
     5221 tattttttca aattcaatgt cgatttgttc tttaatttta tcccagtatt taattgctcc
     5281 tatagtaagc actaccatat cagtcactca ctttcattta ataacccacc tgatatatat
     5341 tgctaagaaa atctacctta tcgttattat tatatgcatg ataaaatcac gggttacatt
     5401 gtccgtcata tttgaataac agactttaat gtcttttctt tattttacta tattcgattg
     5461 aaaaggacaa acccttagga caattcttta ataatttctt ttaaatcctc ttcattaaat
     5521 agataagccg tattgcaaaa atgacatttg acttccccct gtttatcttc ttccaacatt
     5581 tcctttaaat cttttaagcc tatactgatt aaagcttttt taattttttc ccttgagcag
     5641 ttacagtgat attgaaccgg cagcttgtca ttgatttcta tttctcccaa cagaatttcc
     5701 aaaagctctt ccggagtttt accttcatcc aataaagatg taacagaatg tatcttttta
     5761 agattacttt ctaatgttga tatggtttcc tcatccgctt caggcatcag ttgaataata
     5821 aaacctcctg cttttttaat tgaccagtcc ctgtctacca ggactccaag ggcaaccgct
     5881 gaaggtattt gctctgaatt ataaaaataa taagtcaaat cttcagcaat ttctcctgat
     5941 acaagtttag tttgcccaat ataaggctct ttaagtccta aatctttaat aacagtcaga
     6001 taaccatttc caatagccgt ctttacatcc aattttccta ttgaatttaa aggtaagtct
     6061 accaatgaat tgtaaggata tccccttaca tttcccttac tgtctgaggt tacaacaagc
     6121 ccttttattg gaccatcccc cctgatttgc agggtaataa tatctttttc tccttttaac
     6181 atatacccca tcattcctgc tgcagtcaga agtctgccca atgctgctga aaccacaggg
     6241 gaagtattat gaatccttcg tccttcttct accaaattgg ttgttatagc tgcaaaagca
     6301 cgaacttgtt tctttttatc ggttgcacga ataatataat cattcatcat tttacctcct
     6361 gtctgttttc cctttttccc tggctacaaa aaacaatctt tcactgtttt ctttgggtgg
     6421 atcaaaacta taggcatcat agactgccaa aaactccagt cctgcttttt ctatcaagcc
     6481 aatcaactga ctgacagagt acattttttc ataatggatt tcctcatatc tttcataaag
     6541 gtttgtatct tcctggcgta taaacaatgt aagatgatat tcatttaagg ctttatcttc
     6601 atcataaaaa ttattccaga taaaagccgc ttcatcataa ctgtctgcaa aaatattatc
     6661 ccccaaaata tatttatatt tatactctgt attcatatca aaaataaaga gcccgccagg
     6721 attaagataa ttattaaccc atttaaaagt ttgtaataaa tcttcctcat ctaaaatata
     6781 gtttaagctg tcacacaggc tgattataca atctacagta ccatataatt caaattccct
     6841 catatcctgc agcagatata aaatatccag atgctcagaa agggcctttt ctttagcaat
     6901 agtcaacata tccggtgaat tatctattcc aatcatatca atgccttttt ttgcaaacaa
     6961 agtagtcaga tttccggtgc cacaacctaa ttcagccatc agttcaggag aacagttaaa
     7021 tctttcccat attttttcaa tatatttaac ccactcttca tatggaacct cttgcataaa
     7081 taaatcatat actaaagcaa aacttttata gggctccatt cttccacctt ctttttcctt
     7141 ttttcgtcat tttaattttt aattaattat aagaataaat atataaaaaa gcaacaaacc
     7201 cagaattaat tctgagtttg ttgtttcgta atttctttct tacgttttgt catcaatcca
     7261 ccaattctgc ctgtttcctt agctgttaaa gatttccaac cttctttttc gaccttttcc
     7321 agtaaaccta attctgaagc aatctcatat ttaagttttt cgtcttctgt cattggctta
     7381 tttttattct tctttttcat cttgctttac acactccaat caacattcta cccttcttgg
     7441 gcaaatctga aatttttgat ttatttatta aaagtatggt tattcccaag ataaaatata
     7501 catatgatta taaaaaggct tcaaaaacaa t
//
LOCUS       DAOSUD010000087         7497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4911_length_7497_cov_24.266998, whole genome shotgun sequence.
ACCESSION   DAOSUD010000087 DAOSUD010000000
VERSION     DAOSUD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_7497_cov_24.266998"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..2376
                     /locus_tag="PLG49_09360"
     CDS             268..2376
                     /locus_tag="PLG49_09360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOQ17551.1"
                     /translation="MGDEYSGNASELFLAIAVIVSILSFIEAYWLYKWVKKQPASNAR
                     VIEIGELIRKGANTFLAKEYKKLAAFSGIVVIFILLLLPSPIWSGNITDNMIMAAAYI
                     CGTIFSAIAGKVGITIATIANVKAAEASKEGIQKSFMAGFRGGAVMGMAVVGSSLFGV
                     SLIYLLTRDTTALLGFSFGASSLALFAKAGGGIFTKTADVSADLVGKVELGIPEDDPR
                     NPAVIADNVGDNVGDVAGMGADLFDSNVASMAAALVMAVALDQGTGKMLNTSMVFCYG
                     AIGLLASIIGVASARIGKKGNPTRALNNSTYVTTAIFGILTVLATIIFGFEWRIWGAS
                     AVGLLIGVIIGIATNYFTDDTKKPVHAVAKSSESGPAFTVLSGISYGLISTLPAIVGI
                     AFSALIAYKLCEPLGSGYALFGISMSALGMLSIVGMIISNDAYGPIVDNARGLVEMGN
                     LGDKALEVTDSLDSAGNTVKAVTKGFAIGAAGLTVIALLGAFISEVNGAALELGKEAI
                     SNFDIMNPLVFFGLLVGCSIPVVFSAMLMLGVEKNAQRMVAEIHRQFEEIDGLKEGKE
                     GVMPEYDKCIEIATDGAMKELIPAGLMAIIATIVVGIIGGVQAIGGFLTGNIISGLLF
                     ALFMSNTGGLWDNTKKFVEAGNHGGKGSRAHKSAVVGDTVGDPFKDTAGPSINTQITV
                     VSLVASLMSTIFLTLSLFGK"
     gene            2544..3845
                     /locus_tag="PLG49_09365"
     CDS             2544..3845
                     /locus_tag="PLG49_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOQ17552.1"
                     /translation="MEKRPRYLFVISFDGLSSMDFDFIKELPNFKDFLKEASYCKKVY
                     SIYPSLTYPAHTTIVTGKYPKNHGIVDNTRLQINRSSPDWYWYRKDIKGDTLYDLAIR
                     KGMKVVALLWPVTAKSKIQYNVPEIFPNRPWQNQIMVSLMNGSPLYQYKLNKLFGNLR
                     KGIKQPYLDDFTHQSFLYSIKNLEAELFFVHYTDLDSQRHDYGFNSKEAREALLRHDK
                     RLGEFLKVINEKAICDEAAVILLGDHSSLDEDKVIYLNSWLKEKGFIKVNEKGKIINY
                     QAVAKSCGGSAYIYTKDKDKNLLEALRLIFEEINQKFHCFEYIYNREEALKMGADPNC
                     SFMLEANKGYYFLDEITDSFIKEISEEKEREDSSYTRSTHGYSPYKENYTTVFMAKGK
                     GIKKGIVLETMNLIDEGPTLAKLLNLELKDSDGRVLEEILE"
     gene            3872..5119
                     /locus_tag="PLG49_09370"
     CDS             3872..5119
                     /locus_tag="PLG49_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ17553.1"
                     /translation="MKLTKVEKSWALYDWANSAYTMTVTSTILPLYFKNIFASSGGSA
                     ATSTAYWGYANSFATLIIAILAPILGTIADYKGFKKKFFSIFLGLGVIFTFLLTIVPQ
                     DFWLLLLIFYILTAMGFAGANIFYDAFLVDITSEERMDKVSTIGFALGYIGSTIPFII
                     CMTFVVLSQLEIIPLSFEMASKIAFVITALWWGIFSIPMLKTVKQVYGIEMEDHPVRN
                     SFKRLWDTFKNIKQHKQVFIFLIAYFFYIDGVDTIIKMATSYGADLGIGSVTLLVVLL
                     VTQFVAFPFALLYGKLSEKFPGKTMLYVGISIYTFICIYGYFLDSKLDFWILAMLVGT
                     SQGGIQALSRSYYGKIIPKEKSNEFFGFYNIFGKFAAIMGPALMGMISQATGKTNYGV
                     LSVILLFIVGGVVLIMVPNEESA"
     gene            5189..6418
                     /locus_tag="PLG49_09375"
     CDS             5189..6418
                     /locus_tag="PLG49_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ17554.1"
                     /translation="MDKKLLEKYARLLVKTGVNIQKNQILVVQSPVEVADFMRMVVEL
                     AYKEGAKDVQIFWKDELSTKMKYTYAPDEVFEEYPDWQKEFYNSYARKGAAFLNIYAS
                     DPELLKDIDPERVAKYQKVTNIALKEYRDRTMTNQNAWCIASVPTVAWAKKVYPDLPE
                     EEAVESLWQAILKTVRVDQEDPVAAWEEHKRNLEKRMEFLNSKKFRYLHYKNSLGTDL
                     VIELPENHIWTGGADFTQDGVAFIANMPTEEVFTLPKRDGVNGKVVSSMPLNYNGSLI
                     DDFSLTFKDGKIVDFTAAKGYDSLKHLINTDEGSAYLGEVALVPYDSPISNLKILFYN
                     TLFDENASCHLAIGKAYSPCIKGGEKMTPEELKKEGANDSLVHEDFMVGTADLEIMGT
                     TFDGEEIPVFINGNFAF"
     gene            complement(6468..7109)
                     /locus_tag="PLG49_09380"
     CDS             complement(6468..7109)
                     /locus_tag="PLG49_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOQ17555.1"
                     /translation="MKYKKIIWSLMILSFIGTLAIFPHLPEHIPIHWNMHGEIDNYSS
                     KYIALFLAVLPALIYLMMTYLPKIDPKKESYQKHSKAYSIMSATLAVFTILIHWVSLL
                     AALNIIRQTDFFVKLLVGILFVILGNYMTQLRYNYFIGIRLPWTLASETVWRKTHRVG
                     GIAMMLLGLILVLTSFIRGSISAVIFGVSLFSFIFFTFLYAYLEYKKEIKGKS"
     gene            complement(7114..7398)
                     /locus_tag="PLG49_09385"
     CDS             complement(7114..7398)
                     /locus_tag="PLG49_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOQ17556.1"
                     /translation="MDNVFKALSDKNRRQILKLLRKKDLSAGEIAEHFDISKPSLSHH
                     LNILKQANLVQTERQGQNIIYSLNTTAIQELISWIYDFKEKDDSNIKNGG"
BASE COUNT         2417 a         1042 c         1532 g         2506 t
ORIGIN      
        1 ttatagaaaa aattttataa agcattttgt gggttaataa actaatagat attatagtac
       61 aaccttttaa attttgtaca cttcaaataa agcgaatagg aaaaataatt tgtattaatt
      121 tgtaataaaa aaaatgaaag taaaaatgta aaaagcttga attattaatt aaatcgtatt
      181 aatatttagt tggtgttaaa taaaaaagaa aagggggcgt atttagtttt caaacttatt
      241 cattcattaa gaaatatttc gttttcaatg ggggatgaat acagtggaaa tgcaagtgaa
      301 ctttttttgg caattgcagt tattgtttca attttatctt ttatcgaagc ttattggctg
      361 tataaatggg taaaaaagca gccagcttca aatgcacgtg ttatagaaat aggagaactg
      421 atccgaaaag gtgcaaacac atttttagca aaggaatata aaaaattagc agcgttcagt
      481 ggtattgttg ttatttttat tcttttatta cttccttctc cgatttggag tggcaatata
      541 acagacaata tgattatggc tgcagcatat atttgtggaa caattttttc tgcaattgca
      601 ggtaaagtag gtataactat tgcgacgatt gctaatgtga aagctgctga agcttcaaaa
      661 gagggtattc aaaaatcttt tatggcaggt tttcgtggtg gagctgtcat ggggatggca
      721 gttgtaggaa gcagtttatt tggtgtgagt ttaatatatc ttttaaccag agataccact
      781 gctttattag gctttagttt tggtgcaagt tctttagctt tatttgcaaa ggcaggggga
      841 ggtattttta caaagactgc cgatgtaagt gcagacctgg ttggtaaggt agaattaggt
      901 atccctgaag atgatccaag aaatcctgca gttattgcgg ataatgtggg tgataatgta
      961 ggagacgttg ctggaatggg tgcggacttg tttgactcta atgtagcatc aatggctgct
     1021 gctcttgtaa tggcggttgc tttggaccag ggaactggaa aaatgttaaa cacaagtatg
     1081 gtcttttgtt atggagcaat aggacttttg gcttcaatca tcggcgttgc ttcagcaaga
     1141 atcggcaaaa aaggtaatcc tacaagagca ttaaataaca gtacatatgt tacaaccgct
     1201 atttttggca ttttaaccgt tttggcaacc ataatttttg gatttgaatg gcggatttgg
     1261 ggagccagcg cggtaggttt attgattggc gttattattg gtattgcgac caattatttt
     1321 acagatgata ctaaaaaacc agttcatgca gtagcaaaat catcagaatc tggtcctgct
     1381 tttacagttt tatctgggat ttcgtatggt ttaatcagta ctttgccagc tattgtaggt
     1441 attgcttttt ctgccttgat tgcttataaa ttgtgtgagc ctttaggtag cggttatgct
     1501 ttgtttggta tttctatgtc agcattaggc atgctttcaa ttgtaggaat gattatttct
     1561 aatgatgcct atggtcctat cgtcgataat gcaagaggtc tcgtagaaat gggaaattta
     1621 ggcgataaag ctctggaagt aacagattct ctggatagtg ccggaaatac agtaaaagcg
     1681 gttaccaaag gatttgctat tggtgcggca ggtcttaccg ttatagctct tttaggtgcc
     1741 tttatcagcg aagtgaatgg cgctgctctt gaattaggaa aagaagccat cagtaatttt
     1801 gatattatga atcctttagt tttctttggt ttattggttg gctgttctat tccagttgtt
     1861 ttttcagcta tgttaatgtt aggtgtagaa aagaatgcac agaggatggt tgctgaaatt
     1921 catcgtcaat ttgaagaaat tgatggttta aaagaaggaa aagaaggcgt tatgcctgaa
     1981 tatgacaaat gtatagaaat tgctaccgat ggtgctatga aagaattaat accagcaggt
     2041 ttaatggcta ttatagcaac cattgtggtt ggtattattg gtggtgtcca ggctattggg
     2101 ggatttttaa ccggaaatat tataagtggt ttattatttg ccttatttat gtctaacaca
     2161 ggcggactgt gggacaatac taaaaaattc gttgaagcag gtaaccatgg tggaaaaggt
     2221 tcaagagccc ataaatcagc tgttgttggc gatacagtag gagatccttt taaagataca
     2281 gctggtcctt ccattaatac acagatcacg gttgtttctt tagtggcatc cttaatgtca
     2341 accatctttt taaccctttc attatttggt aaatagaatt attcatttaa aattttgata
     2401 tgatatctcc aaaggatagt ctgatgaatg ataatcagtc tgttcattgg agatattttt
     2461 atatatgact gtatatgctc ggaaaattat ggtatgatta attatagagt ataatcatac
     2521 agagcaaatt taggagggca tctatggaga aaagaccaag atatttgttt gtcatttcct
     2581 ttgatggtct ttcaagtatg gattttgatt ttataaagga attgccaaat tttaaagact
     2641 ttcttaaaga agcttcttat tgcaaaaagg tttatagtat ttatccatca ctgacttatc
     2701 cggcccatac aacgattgtc acgggaaaat atccgaaaaa tcatggtatt gttgacaata
     2761 ccaggcttca gattaataga agctctccgg attggtattg gtaccgtaag gatataaaag
     2821 gagatacttt atatgatttg gccattagaa aaggcatgaa agttgttgct ctcttatggc
     2881 cagtaactgc aaaatccaaa attcaataca atgtaccaga gatttttcct aacaggccct
     2941 ggcaaaatca gataatggtt tctttaatga atggaagtcc tttatatcag tataaactca
     3001 ataaattatt tggaaattta agaaaaggta taaagcagcc atatcttgat gattttacgc
     3061 atcaatcttt tttgtatagc ataaaaaatt tagaagccga gctctttttt gttcattata
     3121 cagatttaga tagccaaaga catgattacg gttttaattc aaaagaagcc cgggaagcct
     3181 tattaaggca tgataagagg cttggagaat ttcttaaggt tataaatgaa aaggctattt
     3241 gtgatgaagc ggctgtgatt ttgctgggag atcacagcag tttagatgaa gacaaggtga
     3301 tttacttaaa tagctggtta aaagaaaaag gctttataaa ggtgaatgaa aaaggaaaaa
     3361 taataaatta ccaggctgtt gcaaaaagct gcggaggttc agcttatatt tatacaaaag
     3421 ataaagataa aaatctattg gaagctttgc gtttgatttt tgaggaaatc aatcaaaaat
     3481 ttcattgttt cgagtatatc tataatcgcg aagaagcctt aaagatggga gcagacccaa
     3541 actgcagctt tatgctggaa gccaataaag gttattattt tttagacgaa attacagata
     3601 gttttataaa agaaatttca gaagagaaag aaagagaaga ttcttcttat acacgttcaa
     3661 cgcatggcta ttcaccttat aaagaaaatt atacgacggt ttttatggct aaagggaaag
     3721 ggattaaaaa aggaattgtt cttgaaacaa tgaatttaat tgatgaagga cctactctgg
     3781 caaaactgtt aaatttagaa ttaaaagatt cagatggcag agtccttgaa gaaattctgg
     3841 aataaaaata cattaagaat tgggggataa aatgaaatta acgaaagtag aaaaaagctg
     3901 ggctttatat gattgggcaa attctgcata cactatgacg gttacatcaa cgattttacc
     3961 tttatatttt aaaaatattt ttgcaagcag tggaggcagt gcagcgactt ctacggctta
     4021 ttggggttat gccaattctt ttgcaacttt gattatagcc atattagccc cgatcctggg
     4081 tactatagca gattataaag gatttaaaaa gaaatttttt agtatttttt tgggtttagg
     4141 ggtgattttt actttccttt tgacaattgt accacaagat ttctggcttc tgcttttgat
     4201 tttttatata ttgactgcca tgggttttgc aggtgccaat attttttatg atgcattttt
     4261 agtggatatt acatccgaag aaaggatgga taaggtttct accattggat ttgctttagg
     4321 ttatattggc agtaccattc catttattat ttgtatgact tttgttgttt tatctcagct
     4381 agaaatcatt cctttatctt ttgagatggc aagtaagatt gcatttgtga ttacagcttt
     4441 gtggtggggg attttctcaa tacccatgtt aaaaacagta aaacaagtct atggaataga
     4501 aatggaagac catcccgtcc gcaatagttt taaaagatta tgggatacgt ttaaaaatat
     4561 taagcagcat aagcaggtat ttatcttttt aattgcatac tttttttaca ttgatggagt
     4621 cgataccatt ataaaaatgg ctacttctta tggtgcggat ttgggcattg gttcagtaac
     4681 attgcttgtg gttcttttgg ttacacagtt tgttgctttt ccctttgccc tcttatatgg
     4741 aaaactttcg gagaaatttc ctggtaaaac aatgttatat gtgggtatta gtatttacac
     4801 gttcatctgt atttatggat attttttaga cagtaagctg gatttctgga ttctggctat
     4861 gttggtaggt acttcccagg ggggcattca ggcgcttagc cgttcttatt atggtaaaat
     4921 tattcctaag gaaaaatcga acgagttctt tggcttttat aatatatttg gcaaatttgc
     4981 ggccattatg ggaccagcat taatgggtat gatctctcag gccacaggta aaactaatta
     5041 tggtgttttg agtgttatct tgttatttat cgtcggggga gtggtattaa tcatggtacc
     5101 caatgaagaa agtgcataga aaaaattgac ttgtaaagaa taaagagata tattaataat
     5161 attgacttaa aaatggaagg agatctatat ggataaaaaa ttgcttgaaa agtatgctag
     5221 attattggta aaaacagggg tcaacattca aaaaaatcag attctggttg tacaaagccc
     5281 cgttgaagta gcagatttta tgcgaatggt tgtcgaactt gcttataaag aaggggcaaa
     5341 agacgtacaa atcttttgga aagatgaatt gtctaccaag atgaaatata cttatgctcc
     5401 ggatgaagtt ttcgaagaat atccggattg gcaaaaagaa ttctataatt cttatgcaag
     5461 aaaaggagct gcttttttaa atatatatgc ttcagatcct gaactgctta aggacattga
     5521 tcctgaaagg gttgctaaat atcaaaaggt aaccaatatt gctttaaaag agtacaggga
     5581 cagaactatg accaatcaaa atgcctggtg tattgcgtcc gttcccacag ttgcctgggc
     5641 aaaaaaggtc tatccggatt tacctgaaga agaagcagtg gaaagcttgt ggcaagctat
     5701 tctaaagact gtcagagtag atcaggagga tcctgtagct gcctgggaag agcataaaag
     5761 gaatctggaa aaaagaatgg aatttttaaa ttctaaaaaa tttagatatc ttcattataa
     5821 aaattcttta ggaacggatc ttgtgattga acttcctgaa aatcatattt ggactggtgg
     5881 agcggatttt acgcaagatg gtgtagcctt tattgccaat atgccgactg aagaagtctt
     5941 tactttacca aaaagagacg gagtcaatgg taaagtggtt agttctatgc ctttaaatta
     6001 taatggcagc ttaatagatg acttttcatt gacttttaag gatggaaaaa tagttgattt
     6061 tacagctgca aaaggctatg acagtttaaa acatctgatt aataccgacg aaggttcagc
     6121 atacttgggt gaagttgccc tggtacccta tgattcgcca atttctaact taaagattct
     6181 cttttataat accttatttg acgaaaatgc ttcttgtcat ttagccattg gaaaagctta
     6241 ttccccatgt ataaagggcg gggaaaagat gacgccagag gaactgaaaa aagagggagc
     6301 caatgattcg cttgtacatg aagattttat ggttggtacc gcagaccttg aaatcatggg
     6361 aaccactttt gatggggaag aaattcctgt ctttataaac ggtaattttg ctttttaact
     6421 gactaaaaaa tcctaagaga ccgtattctc ttaggatttt ttctttttta agatttgcct
     6481 tttatttctt ttttatattc aaggtatgcg tataaaaatg taaagaatat aaaagagaaa
     6541 agtgaaacac caaaaataac agcgctaatc gaacctctta taaatgaagt taagacaagg
     6601 ataaggccta aaagcatcat tgcgattcca ccaactctgt gagtctttct ccagacggtt
     6661 tcgctggcga gtgtccaggg taaccttatt cctataaaat aattatagcg taattgggtc
     6721 atgtaattgc ccaaaataac aaataaaata ccaaccaata gttttacaaa aaagtcggtc
     6781 tgtctaatga tatttaaagc agctaaaaga ctaacccaat gaataagaat tgtaaatacg
     6841 gctaaagtgg ctgacataat actgtaagct tttgaatgtt tttgatagct ttcttttttt
     6901 gggtcaattt tggggaggta tgtcatcata agatagatta gtgcagggag gacagccagg
     6961 aagagagcaa tatattttga actataattg tcaatttccc cgtgcatatt ccaatgaatt
     7021 ggaatatgtt caggtaagtg cgggaagatt gcaagggtgc caataaaaga aagaatcatt
     7081 aaggaccaaa tgattttttt atatttcatt ttatcatcct ccattcttaa tatttgaatc
     7141 atctttttcc ttgaaatcat aaatccagct aatcaactcc tgtatggcag tagtatttaa
     7201 agaataaatg atattttgtc cttgtctttc agtttgtact aaattggcct gttttaaaat
     7261 atttaaatga tgacttaaag aaggtttact gatatcaaaa tgttctgcaa tttctcctgc
     7321 ggataaatct tttttccgaa gtaactttag tatttgcctt ctatttttat ctgataaagc
     7381 tttaaataca ttatccaatt gatagcctcc tttctttaga tatttagata attatctaaa
     7441 tatctaatta aataataaca tgatttttat aataatgcaa tagaaaaatc tatttgg
//
LOCUS       DAOSUD010000088         7451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4932_length_7451_cov_26.853705, whole genome shotgun sequence.
ACCESSION   DAOSUD010000088 DAOSUD010000000
VERSION     DAOSUD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_7451_cov_26.853705"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1614
                     /gene="brnQ"
                     /locus_tag="PLG49_09390"
     CDS             259..1614
                     /gene="brnQ"
                     /locus_tag="PLG49_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HOQ17557.1"
                     /translation="MKERLGLKSNILIGSLLFGLFFGAGNLIFPVYMGQLAGQKTLEA
                     TVGFLITGVGLPLLGVIASALSKSDSLFDMAKPVSKAYSVFFTCMLYLTIGPLFAIPR
                     TASVAFEVGLNPFVPKEYLQLALFIFSFLFFAMTLYFSLKPSRILDWVGKYLTPVFLL
                     LLSVLIFAVFLKPMGQANQYEAQGAYVQKAFFTGLLEGYNTMDALASLAFAIVIISNI
                     EKLGVRDSKRKAIETAKSGLVCVIGMGLIYTSLAYMGATSLGAVGRASNGGQIMSLVS
                     EHYFGNTGKILLAAIVTVACLKTAIGLITSCSQMFSRLFPKSLSYNQYAVFFTIISFI
                     IANFGLNKIIQLSIPVLMFLYPLAIVLIVLSLLSPVLNKAEEVYKWTTVLTVFASFFD
                     FCNALPDVIKKTVVINSLIEFARTYLPGFNIGFGWLIPALAGFITGFTLWKVRGRSLE
                     Q"
     gene            1906..3126
                     /locus_tag="PLG49_09395"
     CDS             1906..3126
                     /locus_tag="PLG49_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOQ17558.1"
                     /translation="MSEMINNREYRQKVLKELITELHNGKSVDEIKPRFEELIQGISA
                     AEIAEMEKALIMEGMPVEEIQRLCDVHAAVFKGSIEEIHRPQKPEEVPGHPVHTFKLE
                     NEAIRKLVNTEIKPQLERYKNGETDSLKALKEGFEKLWEIDKHYLRKENLLFPYLEKY
                     GITAPPKVMWGVDDEIREGIKEIRSSLSNHTIDLGKAEETINKVMEMIFKEENILFPM
                     ALDSLTEDEWLEIVNASKEFGYCLINPKGEWKPARVDIAKKEQAEGNQPVGDKGYIEF
                     DAGCMTAEEINALLNTLPIDITFVDKDGAVKYFTQGKERIFARPKTILGRQVQNCHPP
                     ASMHIVEKIVEDLKAGRKDHEDFWIKMGDKYVFIRYFAVRNKEGEFLGIIEVTQDIAP
                     IQKITGEKRLLSEE"
     gene            3311..3490
                     /locus_tag="PLG49_09400"
     CDS             3311..3490
                     /locus_tag="PLG49_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ17559.1"
                     /translation="MLIKLQGIKKAFGEKKIFNHLNLEIKRGEIFALIGPNGVGKTTL
                     MRRYLTGIGILKGNI"
     gene            3508..4005
                     /locus_tag="PLG49_09405"
     CDS             3508..4005
                     /locus_tag="PLG49_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ17560.1"
                     /translation="MGYSPETPVFPEILSGRQVLEYYMEVRGFDKSFYRKESVRLMEK
                     VGLSLKDTRVKNYSKGMKQRLAVAQALIGDPDLLLLDEPSAGQDFLGQQQMQDLIAEL
                     KKDKKGILLNSHLLYDVEKVADRGIIIMNEKICREFTRDDFKKISLADMFLELAKEVR
                     YEGSY"
     gene            3989..4756
                     /locus_tag="PLG49_09410"
     CDS             3989..4756
                     /locus_tag="PLG49_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17561.1"
                     /translation="MKAVIKLHLKENIKKNSFIIFAVLGSIVTLVVLSQMQFTVNGVQ
                     ATSDYAIYGIQWRILSLMASLAGVALSMNVISNHKEGSKRELLKLHGLPVYKQYLSLI
                     SGNLIVTSIMALILCTGLFIQILIKGGNMTVSGILAGVFFYLLTITCATLFVSLFTLI
                     FPPVEDALLEILAIALGSLRGTLHLIVGNMGGLFGTVTSFFLKLIPPLDAFGQLTRDF
                     FMDSFSDYRTLFSCLFYLWALTGISYFLTRVVTRNED"
     gene            4871..5956
                     /locus_tag="PLG49_09415"
     CDS             4871..5956
                     /locus_tag="PLG49_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ17562.1"
                     /translation="MDFVPMENPINPDDLNWEAIHELGGYGMVIDNEGNIIKRYYQEE
                     DKKSYTYQELMDLFNLRRNDKTTFSYSTVDNNKLLIVYPSSMMTATPTINARALQKTS
                     PHYIQLVFIFALLIYIFLVYIIISILTKRLKKEFNLIKAEEDKRKSLFFRGLAHDVKT
                     PLSTVITYSKALEDGIVDKSELKNYYEVIYKNGILLKERIDNMLSLTTLGDEGIFSPQ
                     EADLLEEIRRFIGDNYSWFVNHKAGIHIEFSDDARFITRYDKKLFIRLLENLLQNSVY
                     HNNKPVNIFIEWENKERCLIIYDDGVGVPEKIRPYMWEPMVTGEESRTGEKLRGMGLA
                     NVKKIVELHGWKIEYDDGKFKIKIKQR"
     gene            6061..6486
                     /locus_tag="PLG49_09420"
     CDS             6061..6486
                     /locus_tag="PLG49_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOQ17563.1"
                     /translation="MFIFETIQLIEQLEQIILASEKVNGFDLHAINEIFRTMHTIKGS
                     SAMMIFNNISTVSHSMEDIFYFLREENPENIEWSKLFDLVLEVVDYIKDEINKIKNGD
                     DTATSPEKLLEELKNYLFSLKSQNKSTSIEQGKQTEEKT"
     gene            6571..>7451
                     /locus_tag="PLG49_09425"
     CDS             6571..>7451
                     /locus_tag="PLG49_09425"
                     /inference="COORDINATES: protein motif:HMM:NF018851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17564.1"
                     /translation="MENIRAYTIIHNIKDMTDDYYFFPEDIMDNDDTCEQIRKEGFTI
                     YLKTDKSYEEMHAFFMKTIFLRDLELKELENDDEFKRFSKTKEAEGSIVLASDPVKTP
                     KLVDEEKVDNGKDRQVAATTQSFISVNVTKLDQLMDLVGEMVVAEAMVIQNPDLQGLE
                     LDNFHKAARQLNKITNVEKLTSNAEEILKFIDEKVSPDYDMLEQTGEQYDQDAHFVKT
                     LTEDFATAASQIADSIESIVKSIEQVAATVEEANASSQEISSNASESTRALEEVAKTA
                     QSQAEMAEKLMHLVEKF"
BASE COUNT         2503 a         1073 c         1540 g         2335 t
ORIGIN      
        1 ttaaaagaca gagttctaaa gaaactctgt cttttaattt atccaaaatg cagatcaaag
       61 gaatttttat tttttatcaa ggaaaatatt tgcttaaaaa tacatctttt ttaaattcat
      121 atgtcgtaaa cagaaaaaat ttgttaaaat aattaattat aataatatca aataatcttg
      181 ataaaaataa agtacttttt tatttggtat tttggaaaaa gttcgtacta tacttaaagc
      241 atgtagggag ttttggctat gaaagaaagg cttggtctta aatccaacat attaatcggt
      301 tccttattat ttggattgtt ttttggggca ggaaatctaa tttttcctgt ttatatgggt
      361 caattggctg gccaaaagac attagaggcg acagtcggtt ttttaataac tggagtggga
      421 cttccactat taggtgttat tgcttctgcc ctttcgaaaa gtgacagttt atttgatatg
      481 gcgaagccag tcagcaaagc ctattcggta ttttttacgt gtatgctgta cttgacgata
      541 gggcctttat ttgcgattcc tcgtacggcc agtgtagctt ttgaggtggg attaaatcct
      601 tttgttccaa aggaatatct tcagcttgct ttatttatat tttcatttct tttctttgcc
      661 atgacgcttt atttttccct taaaccttcc agaattcttg attgggtggg gaaatatctt
      721 acaccagttt ttttgcttct attgtcggta ttaatttttg ctgtcttttt aaagccgatg
      781 ggacaagcaa accaatacga agctcagggt gcttatgttc aaaaggcatt ttttactggt
      841 ttattagagg gctataatac aatggatgcc ttagcttccc tggcttttgc tattgtcatt
      901 atctccaata ttgaaaagct gggggttaga gattctaaga gaaaggccat agaaacagct
      961 aaatcaggcc tggtttgtgt gataggaatg ggtcttattt atacctcctt agcttatatg
     1021 ggagctacaa gcttaggggc tgtcggcaga gcttcaaatg gcgggcagat tatgtctttg
     1081 gtcagtgaac attactttgg caatacaggc aagattttat tagctgctat tgtaacggtt
     1141 gcctgtttaa agacagcgat tggtttaatt acttcctgtt cccagatgtt tagcaggctg
     1201 tttccaaagt ctttgtctta caatcagtat gcggttttct ttacgataat atcttttata
     1261 attgctaatt ttggtcttaa taaaataatt cagctttcta taccggtgtt gatgttttta
     1321 tatccgctgg ctattgtatt gattgtttta tcattgcttt ctccagtgct aaataaagct
     1381 gaagaagttt ataagtggac aacagttttg acagtctttg catccttctt tgatttttgt
     1441 aatgcactgc cagatgtaat aaagaagact gttgtaatca acagcctgat agaatttgcc
     1501 agaacttatc tgccaggttt taatattgga tttggctggc ttattcctgc cctggctggc
     1561 tttatcacag gatttacctt atggaaagtc cggggaagaa gtttggaaca atgattttaa
     1621 tatctaataa aaataaggac atctaaaacg aataaaagcc acctatgcct ttagaggaat
     1681 aaggggcttt ttcataaaac ttaagtttga ataaaagagg ctaaagttgg cagactagat
     1741 aatgttatta tgttttctta aaagattggc gatatatgaa atacagcaca aatattaaaa
     1801 gcaacataat ttgtctgata attgtatttt tgctgtaaaa aagcttgtaa atgtgttacg
     1861 attataatta atctgaaaat tatttaaaaa ggggatgatg gctttatgag tgaaatgatt
     1921 aataaccgtg agtacaggca aaaagtatta aaggaactca ttactgaact gcataatggc
     1981 aaaagtgttg atgaaattaa acctaggttt gaagaactca ttcaaggcat ttcagctgct
     2041 gaaattgctg aaatggaaaa agctttaatt atggaaggta tgcctgtgga agagattcaa
     2101 agactttgtg atgttcatgc agcagttttt aaaggctcca ttgaagaaat ccacagacct
     2161 cagaaacccg aagaagtgcc agggcatccc gttcatacgt ttaaacttga aaatgaagcc
     2221 atcagaaaac ttgtaaacac tgaaattaag ccgcaattag agcgctataa aaatggtgaa
     2281 acggatagtc ttaaggcgct taaagagggc tttgaaaagc tttgggagat tgacaaacat
     2341 tatttgcgaa aagaaaattt attgttcccc tatcttgaaa aatacggaat caccgcacca
     2401 cctaaagtca tgtggggcgt ggatgatgaa atccgagaag gtattaaaga gataagaagc
     2461 agcctttcaa atcataccat tgatttggga aaagcagagg aaaccattaa taaagtcatg
     2521 gaaatgattt ttaaagaaga aaatattctt ttccccatgg ctttagatag tctgacggaa
     2581 gacgaatggc ttgaaattgt caatgccagc aaagaatttg gatattgctt gataaaccca
     2641 aaaggagaat ggaagcctgc acgggtagat attgcaaaga aagagcaagc agaaggcaat
     2701 cagcctgtgg gagataaagg ttatatcgag tttgacgcag gatgtatgac agcagaagaa
     2761 atcaatgcct tattgaatac tttgcccatt gatattactt ttgtggacaa ggatggtgct
     2821 gttaaatatt ttactcaggg aaaagaaagg atatttgccc gccccaagac cattcttgga
     2881 aggcaggtgc aaaactgtca tcccccagca agtatgcata ttgtagaaaa aattgtggaa
     2941 gatttaaaag caggacgaaa ggatcatgag gatttctgga ttaagatggg cgataaatat
     3001 gtttttatca gatattttgc tgtaagaaac aaagaaggtg aattcttagg tattatcgaa
     3061 gtcacacaag atattgcacc gatacaaaaa attacaggtg aaaaacgcct tctttcggaa
     3121 gaataattgc atagaggata tgacaggctt tccgctactg gagtatatcc ggagctggaa
     3181 agctttttta ttttaaatga ttatcctaag cgcaggaact atcctgaaga tttattattt
     3241 aaggttaatt taagatagat caaagttaga ttaaagttta atgttttata ctatagctga
     3301 ggtgatgaaa atgttaatta agttgcaggg cataaaaaag gcctttggag aaaaaaagat
     3361 ttttaatcat ctgaatctgg agattaaaag aggggaaatc tttgccttaa tagggccaaa
     3421 tggggttggt aaaacaaccc ttatgcggag atacttaact gggataggga ttttgaaggg
     3481 gaatatttaa aagaagaggg cataaagatt ggctactcac ctgaaacacc tgtctttcct
     3541 gaaattttaa gtggacgtca ggttttagaa tactacatgg aggtaagagg ctttgataaa
     3601 tctttctaca gaaaggaaag tgtcaggtta atggagaagg ttggtttatc cttaaaagat
     3661 acccgtgtaa aaaattattc caagggcatg aagcaaagac ttgccgtggc gcaggctttg
     3721 ataggggatc cggatttgct gttactggat gaaccttctg cagggcaaga ttttttagga
     3781 cagcagcaaa tgcaggattt aattgcagaa ttaaaaaagg acaaaaaggg tattttgctg
     3841 aattcacatt tactttatga tgtcgaaaag gttgcggaca ggggcattat catcatgaat
     3901 gaaaagattt gcagggaatt tacaagagat gattttaaaa aaataagtct ggcagatatg
     3961 tttttagaac tggcaaagga ggttcgttat gaaggcagtt attaaactac atttaaaaga
     4021 aaatattaag aaaaattctt tcattatttt tgctgtttta ggaagcattg taactttagt
     4081 tgtcttatct cagatgcagt ttactgttaa tggggttcag gcaacttcag actatgcaat
     4141 ttacggaata cagtggagaa ttctttcgct tatggcgtct ctggctggcg ttgccttatc
     4201 aatgaatgtt atatctaatc ataaggaagg cagtaaaagg gagcttttaa aactccacgg
     4261 tttacctgtt tacaaacaat atctaagctt aatatccggc aatctaatcg ttacatcaat
     4321 tatggcttta atcctttgta cgggtctgtt tattcaaatt ctgataaaag gcgggaatat
     4381 gacggtttcg ggtattttgg caggagtatt tttctattta ttaaccatca catgcgcaac
     4441 actttttgta tccttgttca ctttaatatt tccgccagtt gaggatgcct tgcttgagat
     4501 attagccatt gcgctgggca gcctgagagg gactttgcac ttgattgttg gcaatatggg
     4561 tggtttattt ggcacagtga caagcttttt tctgaaactt atccctcctt tagatgcttt
     4621 tggtcaattg acaagagact tttttatgga cagtttttcg gattaccgga ccctttttag
     4681 ctgtttgttt tacctttggg cattgacggg catcagttac tttttgacca gggtggtgac
     4741 aagaaatgaa gattaaactg atttatttat tcatagggct gcttttgttg tttcttttat
     4801 tattatcaat gcaatctttt taattatttt aggggtaatg cttttatttg tccctaaagg
     4861 ggtcaattta atggactttg ttccaatgga aaatccaatc aatccggatg atttaaattg
     4921 ggaggcaatc catgagcttg gtggctatgg aatggttatt gataatgagg gaaatataat
     4981 taaaagatat tatcaggaag aagataaaaa aagttacact tatcaggaat taatggactt
     5041 atttaacctc agaagaaatg ataaaacgac tttttcctat agtactgtag ataataataa
     5101 actgcttatc gtttaccctt cttcgatgat gacagcaaca cccacgataa atgcaagagc
     5161 actgcaaaag acaagtcctc attatattca attggttttt atttttgcct tattaattta
     5221 tatctttttg gtatatatca tcatcagtat attgacaaaa agattgaaaa aagaattcaa
     5281 cttaataaaa gccgaagaag ataaaagaaa aagcctcttt ttcagaggtt tagcccacga
     5341 tgttaaaacc cccctgtcaa cggtgattac ttattcaaaa gcccttgaag atggcattgt
     5401 cgataaaagt gagttaaaga actattatga agtgatttat aaaaacggca ttttattaaa
     5461 agaaagaatc gacaatatgt taagccttac aaccttaggg gacgagggta tcttttcacc
     5521 gcaagaagct gaccttttag aagaaataag acgttttata ggagataatt attcctggtt
     5581 tgttaatcac aaggccggca ttcatataga atttagtgat gatgcccgtt ttattacccg
     5641 ttatgataaa aaactcttta taagattatt ggaaaacctg cttcaaaaca gtgtttatca
     5701 taacaataag ccagtgaata tttttattga atgggaaaat aaagaacgat gtttaatcat
     5761 ttatgatgac ggcgtaggcg taccagaaaa aataagacct tatatgtggg agcctatggt
     5821 gacaggagaa gaaagccgaa caggtgaaaa attaagaggc atgggcctgg caaatgtaaa
     5881 gaaaattgta gaacttcatg gctggaagat agagtatgat gatgggaagt ttaaaattaa
     5941 aataaagcaa agatagatga agggtcagtg gtgataaaat aaaaagctca tatttgtatg
     6001 agttttttta attgacaact atatcggatg acgatataat taaatgatcc tatgcaggat
     6061 atgttcatat ttgaaactat tcaacttatt gaacaacttg agcagattat cttagccagt
     6121 gaaaaagtca atggctttga tttacatgca ataaatgaga tatttcgaac catgcataca
     6181 ataaaaggtt catcggctat gatgatattt aataatattt ctacggtttc tcatagtatg
     6241 gaggatatat tctacttttt gcgggaagaa aatccagaaa atatagaatg gtctaaactt
     6301 tttgatttgg ttttagaagt tgtagattat ataaaagatg aaataaataa gattaaaaat
     6361 ggtgatgaca cggccactag tcctgaaaag ttattggagg aactaaagaa ttacttgttc
     6421 agtttaaaaa gtcagaataa gagtacaagt attgaacagg gaaagcagac agaagaaaaa
     6481 acttagaatt atatcgatcc agataaaatt acacccaatc attatcaata cgcctataaa
     6541 gcaacaatat tttttgatga aggctgtgaa atggaaaata tcagggcata tacaattatc
     6601 cataatatta aggacatgac agatgattat tactttttcc ctgaggatat tatggacaat
     6661 gatgatacct gcgagcaaat acgtaaggaa ggtttcacaa tatacttaaa aacggataaa
     6721 agctacgaag aaatgcatgc attctttatg aaaactatat ttctcagaga tttagaatta
     6781 aaagaattgg aaaatgatga tgagttcaaa cgatttagca aaacaaaaga agctgaaggt
     6841 agcattgttt tagccagtga tccggttaaa actcccaaat tagtggatga ggaaaaagtt
     6901 gataatggta aagacagaca agtagctgcg acaacacaaa gctttataag tgttaacgta
     6961 actaaattag atcagttaat ggatttagtt ggagagatgg ttgttgcaga agcaatggtt
     7021 attcaaaatc cggatttaca aggattagag ctggacaatt ttcacaaagc ggccaggcag
     7081 ttaaataaga ttacaaatgt tgagaagctt acttctaatg ctgaagaaat attaaaattt
     7141 attgatgaaa aggtttcgcc agattacgat atgttggagc aaactggaga acagtatgat
     7201 caggatgctc actttgtaaa aactttgacc gaagattttg caactgctgc ttctcaaatt
     7261 gcagattcta ttgaaagcat tgttaaatcg attgagcaag tggctgctac cgtggaagaa
     7321 gccaatgcat cttctcagga aatcagcagc aacgcctcag aatcaacaag agctctggaa
     7381 gaagtggcaa aaacagcaca atcacaggca gaaatggctg aaaaactaat gcatctggta
     7441 gagaaattta g
//
LOCUS       DAOSUD010000089         7444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4940_length_7444_cov_22.941535, whole genome shotgun sequence.
ACCESSION   DAOSUD010000089 DAOSUD010000000
VERSION     DAOSUD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_7444_cov_22.941535"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLG49_09430"
     CDS             <1..496
                     /locus_tag="PLG49_09430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ17565.1"
                     /translation="FSWIIFGLTPLAWIMVSDTIATSFVSPLGEEFGWRGFLTGHLSK
                     HFSILKTAVIVGLIWAGWHYWFYLIPGEFSIEVPVLFFILSCIADSLWITYFYVKNKE
                     SVLMGILFHFAYNLSYRIIPIRPSFYFGNGLTYLITVIIELILAVFVILNKWSVPIHC
                     KKLQ"
     gene            908..3220
                     /locus_tag="PLG49_09435"
     CDS             908..3220
                     /locus_tag="PLG49_09435"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HOQ17566.1"
                     /translation="MITKIKKRDGREVPFNIEKITNAIFKATKAAGQEDYDLAMILAN
                     KVVQRLHEDPNKAIPTVEEIQDIVEKVLIEEGHANTAKKYILYRAERTRVREMNTRLM
                     KIYEDLTFKESKDNDLKRENANIDGDTAMGTMLKYGSEGAKQFCEMYILKPEHAQAHK
                     EGDIHIHDLDFLTLTTTCCQIDIVKLFKDGFSTGHGHLREPNDIHSYSALACIAIQSN
                     QNDQHGGQSIPNFDYGMALGVAKTYARLYRQNLAKALKLFTANEDAVNIVNEILQKIT
                     KETNLRPTLAPNEQYLKLEQEYLMKYIGDADIISKAQDFAIKEAEVEIDRNTYQAMEA
                     FVHNLNTMHSRAGAQVPFSSINYGTDTSPEGRLVIKNILLATEAGLGNGETPIFPVHI
                     FKVKEGINYNPGDPNYDMFKLACRVSAKRLFPNFSFIDAPFNLKYYRAGQPETEIAYM
                     GCRTRVIGNVHDPSREIIYGRGNLSFTTVNLPRIAIRSNGDMDYFFEELDKKIDLIID
                     QLLERYKIQARKKVKNFPFLMGQGVWIDSDKLNWEDEIREVLKHGTLSMGFIGLAECL
                     KALIGKHHGESEDAQQLGLKIIRHMRQRMDDTSKKYKMNFTLLATPAEGLSGRFVRMD
                     RELFGSIEGITDREYYTNSFHIPVYYEINAFDKIRLEAPYHELTNAGHITYVEMDGDP
                     TQNLQAFEKIIRAMKEAGIGYGSINHPVDRDPVCGFTGIIGNTCPSCGREEGDIKFER
                     IRRITGYLVGSLERFNDAKRAEERDRVKHI"
     gene            3282..3791
                     /gene="nrdG"
                     /locus_tag="PLG49_09440"
     CDS             3282..3791
                     /gene="nrdG"
                     /locus_tag="PLG49_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOQ17567.1"
                     /translation="MESYIKIAGLIKESIVDGPGIRMVVFAQGCKHHCPGCHNPETHS
                     FEGGQLISIDSILEEAKKNPLLDGITFSGGDPFEQAEAFAQLAKKAKKLNLDIVTYTG
                     YTYEFLLKNLSSHKGWAELLDETDILVDGKFELDKRNLLLKFRGSENQRIIDVKKTKA
                     KKKIVLFDD"
     gene            3940..4773
                     /locus_tag="PLG49_09445"
     CDS             3940..4773
                     /locus_tag="PLG49_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ17568.1"
                     /translation="MALKFISWNIDSLNAALTGTSDRSVLSRTVLNKIVEHNPDVIAF
                     QETKLTADGPTKKHLEILEKLFPDYKIVWNSSVKPARKGYSGTMFLYKNHLNPLVSFP
                     KIGAPGTMDWEGRIITLEFEDFYLTQVYTPNAGDALNRLDDRQIWDVKYAEYLVSLDN
                     KKPVIASGDFNVAHKEIDLAHPESNRRSAGFTDEERAGFSNLLSKGFTDTFRYLHGDI
                     EGVYTWWSQRIRTSKINNSGWRIDYWLVSDRIAEKVRKSEIIDSGPRQDHAPILLEMD
                     I"
     gene            4890..5623
                     /gene="ygiD"
                     /locus_tag="PLG49_09450"
                     /pseudo
     CDS             4890..5623
                     /gene="ygiD"
                     /locus_tag="PLG49_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
     gene            5799..6374
                     /locus_tag="PLG49_09455"
     CDS             5799..6374
                     /locus_tag="PLG49_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ17569.1"
                     /translation="MELGKQIKKYRKALSLSQDALAEKIFVSRQTISNWENDKSYPDI
                     NSLILLSEVFNTSIDNLVKGDVETMKKEISQFERKRFNELKNFYAVLLILDIVIPTPL
                     FYFLKSAGVVIWVLISALTIYIAFQLEKEKKKYDIQTYREIVAFLEGKNLNEIEKAKE
                     EGKRLYQKLIAAIASLTVAMLIAIGFNCILR"
     gene            complement(6386..7147)
                     /locus_tag="PLG49_09460"
     CDS             complement(6386..7147)
                     /locus_tag="PLG49_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOQ17570.1"
                     /translation="MIGLYFSGTGNSKYCIEKFLHQYDKTAKAYPIEDISSFNEINEH
                     EIIVMAYPVQYSNIPKILSDFIENHKTLWQGKQIFIIATMALFSGDGTGVLARKLKKY
                     GAQIIGGIHITMPDSIADEKVLKRSFKQNKKLISRADEKIKKAVEKIKNNKPPQEGIG
                     LFCQLAGLLGQRLYFYHKTKEYSDKLKIDSEKCIGCGKCVKLCPMKNLELKNKTLISK
                     NKCTLCYRCINDCPKQALTLLGKQVISQHKIDKYL"
BASE COUNT         2510 a         1132 c         1425 g         2377 t
ORIGIN      
        1 gtttagctgg attatttttg gtcttacacc tcttgcctgg attatggttt cagatactat
       61 tgccacttct tttgtttcac ctctgggaga agaatttggc tggagaggtt ttttgacagg
      121 gcacctttca aaacatttta gcattttaaa aacagcagtt atagtcggat tgatttgggc
      181 aggctggcat tattggtttt atttaatacc aggagaattt agtattgaag tacctgtttt
      241 attttttata ttatcctgta ttgcagatag cttatggatt acctacttct atgtaaaaaa
      301 taaagagagt gtattaatgg gaatactatt tcattttgca tataatcttt cctatcgcat
      361 cattccaata agacccagtt tttacttcgg aaatggatta acttatttaa taactgttat
      421 aatagaactt atacttgctg ttttcgttat attgaataag tggtctgttc cgatacattg
      481 taaaaagctc cagtaatgat tttcttaccg ttaaaaaaac taccatatac tgccacctgc
      541 tgatatcaaa gcttattcac gggctatttt tgagcaaatc aattgatata attaattgat
      601 atatgtaata taaaaaaatt atatatgtta aaataagcca ctttctatca tattttctga
      661 tagagggtgg tttttttgaa cttgtcaagt cttgattcaa tatatagtat tatgatagta
      721 taaaaacaac taaatattgt tatattaaag aggctgacaa tgatgattat aaatcagtcc
      781 gattattttt taaagtttcg ccggcaatca taatgttttt acttgtggca gtctcttgac
      841 ttttgctggg caattttaca tatttaacaa acatatgaaa aatttatatg aaggaagggt
      901 aagtcttatg attacaaaaa tcaaaaaaag agatggaaga gaagtacctt tcaacatcga
      961 aaaaatcacc aatgctattt ttaaagcaac caaggctgca ggacaagaag actatgacct
     1021 ggctatgatt ttagctaaca aagtagttca acgtttacat gaagatccga ataaagcaat
     1081 accaacagtc gaggaaatcc aggatattgt agaaaaggtg ttaatagaag aggggcatgc
     1141 caatactgca aaaaagtaca tactttaccg ggcagaaaga actcgtgtca gggaaatgaa
     1201 tacccggtta atgaaaatat acgaagactt aacctttaaa gaatctaaag acaatgattt
     1261 aaaacgtgaa aatgccaata ttgatgggga tacggctatg ggaaccatgc tcaaatatgg
     1321 ttctgaaggg gcaaaacaat tttgtgaaat gtatatatta aagcctgaac atgcgcaagc
     1381 tcataaagaa ggcgatatcc atattcacga tttagatttc cttactctga caacgacttg
     1441 ttgtcaaatt gatattgtta agctgtttaa agatggtttt agtacaggtc acggacattt
     1501 aagggagcct aacgatatac acagctattc tgctttggct tgtattgcaa ttcagtccaa
     1561 ccaaaatgac caacatggtg gtcaaagtat acccaacttt gattatggta tggctttagg
     1621 cgttgctaaa acctatgcca gattatacag acaaaatctg gccaaagctt taaaactttt
     1681 tactgctaat gaagatgctg taaatatcgt caatgaaatt ttgcaaaaga ttacaaaaga
     1741 aactaatctt cgcccaactt tagctcccaa tgaacagtat ttaaaattgg agcaggaata
     1801 tttaatgaag tatattggcg atgcggatat tatcagtaag gcgcaagact ttgccattaa
     1861 agaggcagaa gtggaaattg accgtaacac ctatcaagcc atggaggctt ttgttcacaa
     1921 tttaaatacg atgcacagca gggcaggagc acaagtacct ttcagttcaa tcaactatgg
     1981 tacagatact tctcctgaag gcagattggt cattaaaaat attcttttag cgacagaagc
     2041 aggtcttggt aacggggaaa caccaatatt tcctgttcat attttcaagg taaaagaagg
     2101 aattaattat aatccagggg atcccaatta tgatatgttt aaactggcct gtagggtaag
     2161 tgctaagaga ttgtttccta acttttcctt tattgatgcg ccttttaatt tgaaatatta
     2221 tagagcaggt caaccagaaa cagaaattgc atatatgggt tgcagaacaa gggttattgg
     2281 taatgttcat gacccatcaa gggaaatcat ttatggaagg ggtaatttaa gttttacgac
     2341 cgttaacctt ccacgaattg ccataagaag caatggagat atggattact tctttgaaga
     2401 attggataaa aaaattgatt taattattga tcagctttta gaaagatata aaattcaggc
     2461 tcgaaaaaag gttaaaaact ttccatttct tatgggacag ggtgtatgga ttgattcaga
     2521 caagctcaat tgggaagatg aaatcaggga agttctgaaa catggaactt tgtcaatggg
     2581 ctttatcggg ctggctgaat gtttaaaagc cttaattggt aagcatcatg gggaatcgga
     2641 agacgctcag cagcttggac ttaaaattat aagacacatg cgccagcgta tggatgatac
     2701 cagtaaaaag tacaagatga actttaccct tcttgccact ccggccgaag gtttatccgg
     2761 cagatttgtt cggatggaca gagaactttt tggttctatt gaagggatta cggacaggga
     2821 gtattataca aacagctttc atattcctgt ttactatgaa attaatgcct ttgataagat
     2881 aagacttgaa gcgccctatc atgaactgac aaatgctgga catattacct atgttgagat
     2941 ggatggggat ccaacacaaa atcttcaggc ctttgaaaaa atcataagag ccatgaaaga
     3001 agcaggtatt ggctatggtt caattaatca ccctgtagac agagatcctg tatgcggatt
     3061 tacaggtatc attggcaaca cttgtccttc ttgcggcagg gaagaaggag acattaagtt
     3121 tgaaaggata agacgtataa cgggttatct tgtaggttct ttagagcgat ttaatgatgc
     3181 caaaagagca gaagaaagag accgggtaaa gcatatataa gatggataat gaaatatctt
     3241 taaaatgatt tctatcttag gaatgataag tgggtgatct gatggaaagc tatatcaaaa
     3301 tagcaggttt aatcaaagaa tctattgtgg atggaccagg aatacggatg gtggtttttg
     3361 cacaaggctg taagcaccac tgccctggct gccacaatcc tgaaacccat tcttttgagg
     3421 gcggtcaatt gataagtatt gattcaattc ttgaagaagc taaaaaaaat ccccttttgg
     3481 atggaattac ctttagtgga ggggatccct ttgagcaggc agaagctttt gcacaactgg
     3541 ctaagaaggc aaaaaaatta aatttagata tagtgacgta tacagggtat acttatgaat
     3601 ttcttttaaa aaatttatcc agtcacaaag gttgggcaga acttctggat gaaaccgata
     3661 ttcttgtaga cggtaaattt gaactggata aaagaaactt gcttttgaaa ttccgaggat
     3721 cagaaaatca aagaataatt gatgtaaaaa agacaaaagc taagaaaaaa atcgttcttt
     3781 ttgatgacta atatttataa aaacctgcag tgttgcaggt ttttatattt ctgaatgcga
     3841 cattatattt acaaattctt tttaataaga taagcttata ttaatcagaa attgtctaaa
     3901 atagatagaa atagatgtta atattaaaaa tatggaggta tggcattgaa gtttatatca
     3961 tggaatatcg attcattaaa tgcagcatta acaggtacat ccgaccgaag tgtattatca
     4021 cggactgttc ttaacaagat tgttgaacac aatccggatg tcattgcttt tcaggaaaca
     4081 aaacttactg cggatggacc tacaaaaaag catttagaga ttttagaaaa gctgtttcca
     4141 gattacaaga ttgtttggaa tagttctgta aagcccgcaa gaaagggtta ttccggtacg
     4201 atgtttttat ataaaaatca cttaaatcct ttggttagct ttcctaaaat cggagctccc
     4261 gggacgatgg actgggaagg ccggattatt acactggaat ttgaagattt ttatttaaca
     4321 caggtatata ctcctaatgc cggagatgct ttaaaccgtc ttgatgacag acaaatatgg
     4381 gatgtaaaat atgcggagta tctggtcagc ctggataata aaaaaccagt tattgcatca
     4441 ggagatttta atgtggcgca taaagaaatt gacctggcac accctgagag caaccgccgt
     4501 tcggcaggtt ttaccgatga agaacgtgca ggcttttcaa atttattatc caaaggcttt
     4561 acagatacct tccgttatct tcatggtgat attgaaggcg tttatacctg gtggtctcag
     4621 cggatcagaa cgagtaaaat caataactct ggctggagaa ttgattactg gttagtcagt
     4681 gacaggattg cagaaaaagt ccgtaaatca gaaatcattg attcaggtcc aaggcaagac
     4741 catgcgccaa ttttgcttga aatggatatt taatggaata agaaatcaaa aaagcatgaa
     4801 taaaatacat ctttccttac tcgaagggaa aataataaag ttatttaaaa ttaatatcag
     4861 ggaaaggaaa gtgagttata gtgtcaaaga tgccagtgat ttttgtagga cacgggtctc
     4921 ccatgaatgc catgaaagag atagctaaaa gaataccaaa accagaagtc atcattcgat
     4981 atctgctcat tggtttacca aaggtacaaa aattatgaat gaagaagcgc ctgaaaccat
     5041 ctatgatatg tatggttttc caaagaaact ttatgaaata gtatatgatg caccaggttc
     5101 tcctgttttt gcaaaggcag ttaaggattt gatttcaaaa gaaacagctt atgacaattc
     5161 atgggggatt aatcatggca gctggtcagt attggttcat atgtacccag acaggaatat
     5221 acctgtattt caaattagtg tggatgctta tgcgccacct caggcccatt atcaaattgg
     5281 tcaggaatta aaatctttaa gagaaaaagg tgctcttatc tttggcacag gcaatatcgt
     5341 tcacaattta agattagtgg attggcatat gggaagtaaa ggttttgact ggacctatga
     5401 attcgatgat tttatatatg ataatattat caataaaaac cacaacaata ttttaaagta
     5461 taatgaaaag tctgatgcgg cacagtatgc tgtaccaaca cctgatcatt tttatccttt
     5521 gttatatgtt cttggagctt cagaggcaga agataaagtg accgttttta ataaatcctg
     5581 tgagcttggt tccctgacca tgacaggtta tatttgggaa taatgatttt cttttaggaa
     5641 tttttgaaga ttcattaaat agctgatgaa atcttgtttt ttaaggatgt aaaagacttt
     5701 tgacaatgaa aaatcagact ttatttgtca aatgtaaaag tcttttgctg gacctgttat
     5761 taccctttgg ttagattaag gttgaaggag gttgaaaaat ggaacttggt aaacagataa
     5821 aaaaataccg gaaagcatta tctttatccc aggatgcact ggcagaaaaa atttttgttt
     5881 caagacaaac gatttcaaat tgggaaaatg ataaaagcta tccggacatc aatagtttaa
     5941 ttttactaag tgaagttttt aacacctcca ttgataatct ggtcaaagga gatgttgaaa
     6001 caatgaaaaa agaaattagt cagttcgaaa gaaaaagatt taatgaatta aaaaactttt
     6061 atgctgtttt attaatttta gatatcgtca ttccaacgcc cctgttttat tttcttaaat
     6121 ctgcaggagt tgtcatttgg gttttaattt cagcgcttac gatttatatt gcttttcagc
     6181 tggaaaaaga aaaaaagaaa tatgacattc agacttacag agaaatcgtt gcatttctgg
     6241 aaggtaaaaa cttaaatgaa attgaaaagg ccaaagaaga aggaaaaaga ttatatcaaa
     6301 aactaattgc agctatagct tctctgaccg tggcaatgtt aatcgcgatt ggatttaatt
     6361 gcattttaag ataagtatct gttttttaca ggtacttatc tattttatgc tggctgataa
     6421 cctgcttacc caataatgtg agggcttgct ttggacaatc attgatgcaa cggtaacaca
     6481 gagtacattt gtttttagag atgagcgttt tattttttaa ttccaggttt ttcattgggc
     6541 atagtttaac gcacttacca cagccaatgc atttttcgga atcaattttt agtttatcgg
     6601 aatattcttt ggttttatga taaaagtaaa gtctttgccc caaaagacct gccagctgac
     6661 agaacaagcc aattccttcc tgtgggggct tgttattttt tattttttct acagcttttt
     6721 taattttttc atctgctctt gatattaact ttttattttg cttaaaagac ctttttagta
     6781 ctttttcatc ggcgatacta tcgggcatag taatatgtat accaccaata atttgggcgc
     6841 catatttttt tagcttcctt gccaatactc ccgtaccatc tccgctaaac aaggccatgg
     6901 tagcaataat aaatatttgt ttgccttgcc aaagcgtttt gtgattttca ataaaatcgc
     6961 ttaaaatttt gggtatatta ctgtactgga ctggataagc catcacaatg atttcgtgtt
     7021 catttatttc attgaatgag ctgatgtctt caatagggta tgccttagct gttttatcat
     7081 attggtgtaa aaatttttct atacaatatt tagagttacc tgttccgcta aaataaagtc
     7141 ctatcaatat agaattcccc aagtccaata attcttatta ttataatgaa acaactgcta
     7201 taaatacatg atattattca tctatgcatt aatcaataat attttcgtat ttatctgttt
     7261 taggattagt aattaacatc tttaagtaaa aataaaatgt taaagcaaat cttttagaac
     7321 caattaagaa aactaagctt atattagcca ggtagtattt tgttttcaaa tgacagtatt
     7381 aataattctt acctaaattt ttattgaata aaaataaaaa taagatatat ttttcttata
     7441 tttg
//
LOCUS       DAOSUD010000090         7428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4950_length_7428_cov_23.450834, whole genome shotgun sequence.
ACCESSION   DAOSUD010000090 DAOSUD010000000
VERSION     DAOSUD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4950_length_7428_cov_23.450834"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="csx2"
                     /locus_tag="PLG49_09465"
     CDS             complement(<1..776)
                     /gene="csx2"
                     /locus_tag="PLG49_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOQ17571.1"
                     /translation="MAKKLFAFLGIGDYKSVEYYFQNKNEGYKTEYIQEAITKLLDEQ
                     DLNVTVFVTEEARKKHWEPESNIGLKSKLEKLNINCKAVDIPDGKANNDVWKIFLNVY
                     NEIDINDEIYVDVTHSLRNIPIIFMSVLNYAKVAKNCIIKGIFYGAYEAKENDRAPIY
                     DLTLFDQIGEWSSGVEQLLMTGECEMFCSTVEKTLGPLLREAKGTDELIKLVKKCSKL
                     IKEFYTDLKLVRGKSVLEDGRKLYQVLCEIKALNTEKHIT"
     gene            complement(826..2901)
                     /locus_tag="PLG49_09470"
     CDS             complement(826..2901)
                     /locus_tag="PLG49_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOQ17572.1"
                     /translation="MGNNENVVYKSKAPYNFIELEDFILDKCREEELLMHERYHDGLK
                     TGCIQYEIKVITPLHISAGKKEFGKEKEDSEEDNSREEELFKNPLGQYVIPGNTIRGL
                     ARYNASIFSFASVINEPKRKKDIENKRFFYRTFASKDANIRKWYSDTLGMKPGQRGSF
                     RYTVLQKVQAGYIRKRGEEYVITPAVKIGNKNVDDESRMKSYMPIHEWELRNLNVQGV
                     YYLYNDNLKKEDYKSDSTLRENRNSQYRPYFIEVRYNVAEGKPKIDINGKFKGMLANS
                     NYINSKRHHYLIFEEDKNSAEIVVSKKLAELYTDDLKYTQERNAASKEIYKEYYELPK
                     EGEVKPVFFVKEGDRLIFGFTPYLRIPAEGDIYDGIPEAHKNYTGTDFIDAMFGWKDF
                     RTKLSFLDAVCESPNPEITKEYEMMLAEPKPSWYKGYLKQRKDTLESYSTEGYEIRGR
                     KFYWMKESLDIQGMEEQEKKERLVTRMKCYAENTKFIGKVKFENLTDEELGLLIYSLK
                     QGDTEGYFNLGKGKPYGFGKCRIRILGLFIEDIKEKYTSFNANYLKEEKTDKYVETFK
                     KYIIEHYRIKVSNVKEIISYREFELSKKVLKNSETRYMKVGEFAKRAELPTLEDAVRE
                     TGNVISTKMKGITQKNGNHEVKRDKRKNHNESNKQHNKKGYYNNTKSLNLNTEAFNIL
                     KNYKFENDE"
     gene            complement(2916..3281)
                     /locus_tag="PLG49_09475"
     CDS             complement(2916..3281)
                     /locus_tag="PLG49_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17573.1"
                     /translation="MKIEKIQSGDIASLIKDFNNYIIYYFDNLKFGKGQIELDTDNVN
                     EAFFFGEGKCLHIYREDGVKGMLYIEEPEDKEFILEEQILEDRILKEPLKVLAVKKYI
                     EYDEDGQAYISRTLPVKFI"
     gene            complement(3288..>3583)
                     /locus_tag="PLG49_09480"
     CDS             complement(3288..>3583)
                     /locus_tag="PLG49_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17574.1"
                     /translation="ALMNDEVVMGDVEIECTFNTSGLNDYKKEIGLLLLVLRDLCKED
                     LSIGSGYAVGRGYIKADALELYDGEKLVFDFKSPDGEILKIFDSYISSLMNVG"
     assembly_gap    3584..3683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3684..4132)
                     /locus_tag="PLG49_09485"
     CDS             complement(<3684..4132)
                     /locus_tag="PLG49_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOQ17575.1"
                     /translation="MIYKNRLFKTIRYNVKATTISPLAIRDDEDNLKIDQLTGKVYIP
                     GSSVAGAFRNYYENYIDKNSNENFNELFGGQKTGMSQIVFYDGYLVNDYVKAMISSRP
                     GIKMDPKRMTVEVSDDVKKSGKKFKRRFLNEGLTFEFVFELNNYEDDA"
     gene            complement(4129..5730)
                     /locus_tag="PLG49_09490"
     CDS             complement(4129..5730)
                     /locus_tag="PLG49_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOQ17576.1"
                     /translation="MHKCIQFDIQALEPLKLGKFERDSNNEYSYSYIPGSVVKGAVAW
                     GLVEEKGFVPKEILNGSSIFYNAYPLLDGNPAIPMMQGYMGDKQEIRSNKDNVRVVHS
                     FNRKIENSIPLNNYEFVVKDAENEKLLLGYNPGKIENLHINKKDALNDSSKLKMFRYE
                     AIRKGECFRAYIRVPEEHYDDIVKILSKDYIYFGGSRGSGYGKCRVTGIKEVSAVCLY
                     ESDLDIKEDLYIYFLSDAILYYDGKVNTYIPENELKEMLGIEGKCEYVESYSGLGVAA
                     SYNTLYRTNTVCYTAVLKGSVIKYRVEGKINPDRIKKLASEGVGLRREDGYGQIAVLG
                     KIQDDMVVSRYRKNDIVEKSDIELTDEDKRVVNMILCNIFKTRSKLQIEKMVIELLKD
                     VNRPQESLQSQIGKLLNIFQNGFYQTEDDFRKYLNEYLEHIRKKKGKDVWHKLYDFTV
                     ANTLDKSSSERLSIQKMLEDFVQNKSNSVFREIENIVSKGITLGRCQYPVESQKAQVL
                     YNLKMGFFISFFEYCLRLKEKEVIS"
     gene            complement(5723..6397)
                     /locus_tag="PLG49_09495"
     CDS             complement(5723..6397)
                     /locus_tag="PLG49_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOQ17577.1"
                     /translation="MEEYTLKIELLTDAVFGSGNSVPGFVDADVLHDEYGFLYINGKT
                     LKGKLGEMASVFVNILMEIDECREIAEILQKKKEELFGVAEKYSHSKLKFSDCEFSKN
                     IKDYFKCYMKDSDIKPEEILEALTHIETQTSINYVSGVAKKNSLRNYRVINRGIVLYS
                     SVYSPKKLDDEDKILLASACSMLRHLGSNETKGKGEVRVSLWLKEKEVTHEYIRLLEK
                     KVKLNA"
     gene            complement(6400..>7428)
                     /locus_tag="PLG49_09500"
     CDS             complement(6400..>7428)
                     /locus_tag="PLG49_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17578.1"
                     /translation="KEELSGNYYSGEAKSKIDMFNQREKSNNQFALNIKDLGVSKNEK
                     SYIAITHIDGNRMGKRISNLRESFRSKYNSENIKEINEQYINALNQFSIDIDKAFKTA
                     FNKMVETVEKNRENLEKEGMELKSSVIPIRKVVLAGDDVCYITDARIALECAYIFLQE
                     LEKHSVMGEKITACAGIAIVKEKYPFFKTYELSEELCKNAKSSIEEGKIESRIDWHIV
                     QGEYNNNLDEIRNTVYKTLDGKDLSMRPLVVSKESDSPNHYSLFRKDIEVIRSRKLPR
                     GKIKGMLKEMKKGEAYLDTYIEINQIYNVLGAHRLNAKSGFLNGKCVLFDAIEALDYF
                     IPFCDEEV"
BASE COUNT         2087 a         1601 c          832 g         2808 t
ORIGIN      
        1 atagtaatat gcttttctgt atttaacgct ttaatttcac acaaaacttg atacaacttt
       61 ctaccgtctt ctaaaactga ttttcctctc acaagtttca aatctgtata aaattctttt
      121 ataagttttg aacatttttt tacaagttta attaactcat ctgttccttt tgcttccctt
      181 aataaaggac ctaaagtttt ttcaacagtg gaacagaaca tctcacattc acccgtcata
      241 agcaattgtt caactcccga actccattct ccaatctgat caaacaatgt caaatcatat
      301 ataggtgctc tatcattttc tttagcttca tacgcgccat aaaatattcc tttaataata
      361 cagttttttg caacctttgc ataatttaaa acagacataa atattattgg tatatttcta
      421 agagagtgag tcacatcaac atatatttca tcatttatat caatctcgtt atatacattt
      481 aaaaaaattt tccacacatc attatttgct ttcccatcag gtatatcaac tgctttgcaa
      541 ttaatgttta atttttccaa tttactttta aggccaatat tactttcagg ttcccaatgc
      601 ttctttcgtg cttcttctgt cacaaatact gtaacattta aatcttgttc atccaataat
      661 ttagtaatag cttcttgtat atactctgtt ttatatcctt cgtttttatt ttgaaaataa
      721 tactctacgc ttttataatc tccaattccc aaaaacgcaa aaagtttttt agccattatt
      781 ctatccccct tgacctctaa actttttcct ctttccaaaa ttgacttatt catcattttc
      841 aaatttataa tttttaagta tattaaaagc ttcagtattt agatttaatg attttgtatt
      901 attgtaataa ccttttttat tatgctgttt atttgattca ttatggttct tccgcttgtc
      961 acgttttacc tcatgattac catttttctg cgttattcct ttcatttttg tagatattac
     1021 atttcccgtt tcacggacag catcttccaa tgtaggcaac tcagccctct ttgcgaactc
     1081 tcctaccttc atatatctag tctctgaatt tttcaaaacc tttttactaa gttcaaactc
     1141 cctgtaactt ataatttctt ttacattgct cacctttatt ctgtaatgtt cgattatgta
     1201 tttcttgaaa gtttccacat acttgtctgt cttttcttcc ttgagataat tagcattaaa
     1261 agaagtgtac ttttctttta tatcttctat aaaaagtccc aaaatccgta tcctgcattt
     1321 accgaatcca taaggctttc ctttacccag gttaaagtat ccttcagtgt ctccttgttt
     1381 taatgagtat atcaaaagtc ccaattcctc atctgtcaaa ttttcaaatt taacttttcc
     1441 tataaattta gtattttctg catagcactt catccttgta acaagtcttt ctttcttttc
     1501 ctgttcttcc attccttgaa tatcaagact ttctttcatc cagtaaaact tccgtcctct
     1561 tatttcgtac ccttctgtgc tatacgattc caaagtgtct tttctttgtt ttaaataacc
     1621 tttataccat gacggttttg gctccgccaa catcatttca tactcttttg ttatttcagg
     1681 atttggactt tcacaaacag catccaaaaa cgaaagcttc gttctaaaat ctttccatcc
     1741 aaacatcgca tcaatgaaat ctgttccggt ataatttttg tgagcctccg gtattccgtc
     1801 atatatatct ccttcggctg gaatcctcag atacggagta aaaccaaaaa taagcctgtc
     1861 accttctttt acaaagaaaa ccggttttac ttctccttct ttgggaagtt cataatattc
     1921 tttataaatc tctttgcttg cagcattgcg ctcttgcgtg tacttcaaat catctgtgta
     1981 aagttctgcc aatttttttg atacaactat ttcagcactg tttttatctt cctcaaatat
     2041 taaatagtgg tgcctcttgc tatttatata atttgaattg gcaagcatac ctttgaactt
     2101 tccgttaata tcaattttag gttttccctc cgcaacatta tatcttactt ctataaagta
     2161 tggtctgtac tgactgttac ggttttctct taaagtcgaa tcacttttgt aatcttcttt
     2221 tttgagattg tcattgtata aataatatac cccttgaacg tttaaatttc ttagttccca
     2281 ctcatgtatt ggcatatacg atttcattct gctttcatcg tccacatttt tgtttcctat
     2341 cttaactgca ggagtaataa catattcctc tcccctttta cgtatgtaac cggcctgaac
     2401 tttttgaaga acagtgtacc tgaaacttcc cctttgaccc ggcttcattc ccaaagtatc
     2461 agaataccac tttctaatat ttgcatcttt ggatgcaaaa gttctgtaaa aaaatctttt
     2521 gttctcaata tctttctttc tttttggctc atttatcact gacgcaaaag aaaatatgga
     2581 agcattgtac cttgcgagac cgcgaattgt atttcccgga ataacatatt gcccaagagg
     2641 atttttaaag agttcttctt ctcttgaatt atcttcctca ctatcttctt tctctttacc
     2701 aaattctttt ttgcctgcag aaatatgaag aggtgttatt accttgattt catactgaat
     2761 acatcctgtc ttcaatccat catgatacct ttcatgcatt aatagttctt cttctctaca
     2821 tttatccaaa atgaagtctt ccaattcaat aaagttatat ggcgctttcg atttatacac
     2881 aacattttca ttattgccca taactcattc ctcccttata tgaactttac cggcaacgtt
     2941 cttgatatat aagcctgtcc atcctcatca tattcaatgt atttttttac agccaaaact
     3001 ttaagaggtt cttttaaaat ccgatcttct aaaatctgtt cttccaaaat aaactcttta
     3061 tcctccggtt cttctatata aagcatccct tttactccgt cttccctgta tatgtgcaaa
     3121 cacttacctt ctccaaagaa aaaagcttca ttaacattat ctgtatccag ttctatttgg
     3181 cccttgccaa atttcaaatt atcaaagtag tatataatgt aattgttaaa atcttttatc
     3241 aaagaagcta tatctccgct ttgaattttt tcaattttca tttgtcatca ccccacgttc
     3301 atcaaactcg atatgtagga atcaaatatt ttcaatatct ctccatccgg tgacttaaaa
     3361 tcaaaaacca atttttcacc atcatacaat tccaaagcat ctgccttgat atatcctctc
     3421 cctacagcat aaccgcttcc gatactcaga tcttctttgc acaaatccct tagcaccaaa
     3481 agcaaaagcc ctatctcttt tttgtaatca tttaaccctg atgtgttaaa ggtacactct
     3541 atctcaacat ctcccattac aacctcatca ttcatcaatg ctcnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnngcatcat cttcataatt gttcagctca aaaacaaact
     3721 caaaagttaa tccttcattt aaaaaacgtc ttttaaattt cttaccggat tttttaacat
     3781 catctgaaac ttcaactgtc attcttttag gatccatctt tattcccggt cttgacgaaa
     3841 tcatcgcttt cacataatca ttaacaagat aaccgtcata aaaaacaatc tgactcattc
     3901 ctgttttttg gccgccgaac aattcattaa aattctcatt ggaattctta tcaatgtaat
     3961 tttcataata gtttcgaaaa gcacctgcaa cagatgaacc gggtatataa actttacctg
     4021 tcagttgatc tattttcaga ttatcttcgt catctctaat tgccaaaggt gaaatcgttg
     4081 ttgctttaac attgtatctt attgttttaa acaatctgtt tttataaatc atgatatcac
     4141 ctctttttct ttcaatctca gacagtattc aaaaaaactg ataaagaagc ccattttaag
     4201 attatataga acttgagctt tttgactttc aacaggatat tggcatctgc ccaatgtaat
     4261 gcctttactc acaatattct caatttcacg gaaaacagaa ttggatttat tctgtacaaa
     4321 atcttcaagc attttttgaa tacttagtct ttctgaactg gacttgtcca aagtgtttgc
     4381 aacagtaaag tcatacaatt tatgccatac gtcctttccc tttttctttc ttatatgttc
     4441 aagatattca ttcaaatatt ttctaaagtc atcttccgtc tggtagaaac cattctgaaa
     4501 aatattaaga agctttccta tttgactttg aagactctct tgcggtctgt ttacatcttt
     4561 tagcagttct attaccattt tttcaatttg aagttttgac cgggtcttaa aaatattaca
     4621 taaaatcata tttacaacac gtttgtcctc atctgtaagt tcaatatctg atttttcaac
     4681 aatatcattt ttcctatatc ttgaaacgac catatcatct tgaattttgc ccaatacggc
     4741 aatttggcca tatccgtcct ctcttcttag cccaacacct tcagatgcca atttttttat
     4801 tctatccggg ttaatctttc cttccactct gtattttatt acacttccct tcaatacagc
     4861 agtgtagcaa actgtatttg ttctgtataa agtattgtaa gacgccgcaa ctccaagacc
     4921 ggaataggac tctacatatt cacatttacc ttcaattcca agcatttctt ttaattcatt
     4981 ttccggtatg tatgtattaa ctttaccgtc gtaatacagt attgcatcgg ataaaaagta
     5041 aatatataaa tcctctttaa tatccaaatc cgattcatac aaacaaacag cagaaacttc
     5101 ttttataccg gttactctgc actttccata gccacttccc cttgaaccgc cgaaataaat
     5161 ataatcttta ctcaatatct ttactatgtc atcataatgc tcctcaggaa ctcgaatata
     5221 ggccctaaag cattcacctt ttcttattgc ctcatatctg aacattttta atttactact
     5281 gtcatttaac gcatcttttt tattgatatg aagattttcg atctttcccg gattatatcc
     5341 cagcaggagc ttttcatttt ccgcatcttt aaccacaaat tcataattgt ttaaaggaat
     5401 gctgttttct atttttctgt taaatgagtg taccacacgg acattatctt tatttgaacg
     5461 tatctcctgc ttatcaccca tatacccctg catcattggt attgcaggat taccatccag
     5521 caaaggataa gcattgtaaa atattgaact gccgttcaat atctccttcg gaacaaagcc
     5581 tttttcttca accaatcccc aagcaacggc tccttttact acagaacccg gaatatatga
     5641 ataactgtac tcattatttg aatccctttc aaatttgcca agtttcaaag gctccagtgc
     5701 ctgaatgtca aactgaatac atttatgcat ttagcttcac cttcttttcc aaaagtctta
     5761 tatattcatg tgttacttct ttttctttca gccaaagtga aaccctgact tctccttttc
     5821 cttttgtttc attggagccc aaatgacgaa gcatcgaaca agccgaagcc aaaagaattt
     5881 tatcctcatc atccagtttc ttaggtgaat aaacagacga ataaagcaca attcccctgt
     5941 ttatcactct ataatttctt aatgaatttt tctttgcaac acctgaaacg taattaatag
     6001 atgtctgagt ttctatatgc gttagtgctt ctaaaatttc ctccggcttg atatctgagt
     6061 ctttcatata acacttgaaa taatctttga tatttttcga aaattcgcag tcggaaaatt
     6121 ttagtttgct atgactgtat ttctcagcca caccaaaaag ctcttctttt ttcttttgta
     6181 atatctctgc aatttctctg cattcatcta tttccattaa tatatttaca aataccgatg
     6241 ccatttctcc cagttttcct tttaaagttt ttccgttgat atataaaaat ccatattcat
     6301 catgcaaaac atcagcatca acaaaccctg gaactgaatt accgcttcca aaaacagcgt
     6361 ccgtcaataa ttcaattttc aatgtatatt cttccatact tacacctcct catcacaaaa
     6421 cggtataaaa taatccaatg cttctattgc atcaaacaaa acacattttc cgtttaggaa
     6481 tccgcttttt gcattaagcc ggtgtgcacc aagtacatta taaatctgat taatttctat
     6541 ataggtatct aaataagcct ctcctttttt catttctttt aacattcctt ttatttttcc
     6601 cctgggtaat tttctcgatc ttattacctc tatatccttc ctaaaaagag aataatgatt
     6661 tggtgaatct gattcttttg aaaccaccaa cggtctcatg gaaaggtctt taccgtcaag
     6721 tgtcttataa accgtgtttc taatctcatc caaattgtta ttgtattccc cttgaactat
     6781 atgccagtca atcctcgatt caattttgcc ttcttcaata cttgattttg catttttaca
     6841 aagttcttcg gataattcgt atgttttaaa aaacggatat ttttctttca caattgcaat
     6901 accggcgcag gccgttatct tttctcccat aacgctatgc ttttccaact cttgcaagaa
     6961 aatataagca cattccaaag caatcctcgc atcagtgata taacaaacat catcgcctgc
     7021 cagcacaact tttcgaatcg gtataactga actcttaagc tccattcctt ctttttccaa
     7081 gttttcacga ttcttttcta ctgtctcaac cattttgtta aaagctgttt taaaagcctt
     7141 gtcaatatct attgaaaact gattcaatgc atttatgtat tgctcattta tttcctttat
     7201 attttcagaa ttatacttgc ttctaaaact ttctctcaag ttgcttatcc gctttcccat
     7261 cctgtttccg tcaatatgtg taatggctat atagcttttt tcatttttac ttacacccaa
     7321 atccttaata tttaaagcaa attgattgtt acttttttcc ctttggttga acatatcaat
     7381 tttggatttt gcctcgccgg aataatagtt tccgcttaat tcttcttt
//
LOCUS       DAOSUD010000091         7416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_4966_length_7416_cov_26.921342, whole genome shotgun sequence.
ACCESSION   DAOSUD010000091 DAOSUD010000000
VERSION     DAOSUD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_7416_cov_26.921342"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1426)
                     /gene="radA"
                     /locus_tag="PLG49_09505"
     CDS             complement(62..1426)
                     /gene="radA"
                     /locus_tag="PLG49_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOQ17579.1"
                     /translation="MAKTKNIFVCQECGYESAKWLGKCPACNGWSTFVEEITDSRKPS
                     VLSGKEPHSYPVILKDIGTDEEERILTGSKELDRVLGGGIVKGSLILVGGDPGIGKST
                     LLLQISSHIGQNGCKILYVSGEESIRQIKMRADRLELGADDLLILSETNIDAIEGIIM
                     KESPDIVIIDSIQTVYKEEISSAPGSVSQVREATSSLMRISKGNNISIIIVGHVTKEG
                     AIAGPKVLEHMVDTVLYFEGERHASYRILRAVKNRFGSTNEIGVFEMQDKGLIEVENP
                     SELMLAGRPVNTPGSVVSCSMEGTRPLLVEVQALVSFTNFGMPRRTATGIDYNRVVML
                     MAVLEKRVGMQLISYDSYVNLTGGIKINEPALDLGIAAAIASSFRDKIIDSQMVVFGE
                     IGLTGEIRGVSMAEKRVAECAKMGFKSCVIPKANLKNMKKLDGIQIYGVENINEALEI
                     IIKN"
     gene            complement(1483..1893)
                     /locus_tag="PLG49_09510"
     CDS             complement(1483..1893)
                     /locus_tag="PLG49_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HOQ17580.1"
                     /translation="MAVVEELIRLEDDNSLSFGNYLMDTKKKVIDFEVDGDLYKVKTY
                     NKITKLEKNGILLYESVPGTTVHNFTMNEKQVTFAVEGENTSQITMELEPEKEYKIYV
                     DNVQVGKVKASLAGKVTFSIDFTTGKQNVRIEKI"
     gene            complement(2002..4446)
                     /locus_tag="PLG49_09515"
     CDS             complement(2002..4446)
                     /locus_tag="PLG49_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOQ17581.1"
                     /translation="MMGKFTRRAQEALQASQQAAAELGHGYVGTEHILIGLLRIDDSV
                     AARALKNQGVSEEAIVDKLKAVIGMGDIATAEPQDFTPRAKRIIEMSFREALKMGAGY
                     IGTEHLLLALLREQDCIAVKLLMSMGVNPQKIYDDIMAMLGENNKGQGGMPINQKGAK
                     NGHSDTPTLDQFSRDLTAMATEQKFDPIIGRDKEIERVIQILSRRTKNNPCLVGEPGV
                     GKTAIAEGLAQKIVEGNVPEIIRNKRVVSLDLSAMIAGSKYRGEFEERIKKALDEIRT
                     AGNVILFIDELHTIIGAGAAEGAIDASNILKPSLARGEIQVIGATTLDEYRKYIEKDA
                     ALERRFQPVKVEEPTEEETIEILKGLRDKYEAHHQVHITDDAIVASVKLSSRYITDRF
                     LPDKAIDLIDEAASKVRLRLYTAPPNVKELEARLEELEKEKEAAIKTEEFEKAGEIKK
                     KQDEIKKLLEQEKNDWKDAYDKSKQEVTEEEIADIVSSWTGIPVKKLAEEESERLKNL
                     EEILHKRVIGQEEAVRAVAKAIRRGRVGLKDPKRPIGSFLFLGPTGVGKTELTKALAE
                     ALFGDENAMVRIDMSEYMEKHSVSKLIGSPPGYVGYDEGGQLSEKIRRKPYSVILFDE
                     VEKAHPDVFNILLQVLDDGHITDSQGRRVDFKNTVVIMTSNIGARNIIEPKKLGFASV
                     ENETKNYEDMKKNVMDEVKKLFRPEFLNRIDEMIVFHPLNQDHIKQIVSVMVEQLAKR
                     IKQNMSISIEITESAKEYLAKEGFDQAYGARPLRRAIQSKIEDKMAEEILDGKIKEGD
                     HIKIDLEENQLKFIRI"
     gene            complement(4476..5498)
                     /locus_tag="PLG49_09520"
     CDS             complement(4476..5498)
                     /locus_tag="PLG49_09520"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOQ17582.1"
                     /translation="MRKWYEDSLDSDIVVSCRIRLARNYKKYPFAVKLSLPQAEKLIE
                     ETKKVLLEGNTILSKEFEYISVYNNSPVNKRAMMESHIISPELVRKNAPCGVLLKRDE
                     SISIMVNEEDHLRIQSMAAGMNLDKAWDLADKIDDVLEESIEYAFDETLGYLTSCPTN
                     VGTGMRASYMLHLPALEWSGQLQNIIQAIGKLGFAVRGLYGEGTKAEGSYYQISNQIT
                     LGQSEKEIIENLNTVTMQIVEQEKNIRENIIKDRKMEFLDKIYRSYGIVKYAKVLSTK
                     EAMTYLSDVKIGYEMGILDEPKPKMNLYSLVMHIQPANLQKKMGISLTGQEIDIERAS
                     FIRQQF"
     gene            complement(5495..6013)
                     /locus_tag="PLG49_09525"
     CDS             complement(5495..6013)
                     /locus_tag="PLG49_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOQ17583.1"
                     /translation="MLCERCKKNIANVHVIQINNNEKVELYLCEQCAKETESIHFDTP
                     ISFQDFLTGFLDMPLMGKVPSKEYSSQGTTLQCPSCKMTYDEFSKIGRFGCAQCYTAF
                     SSRLMPIIKNVHGNNEHVGKLPSSAAGELKIKRELEMLKKELKEAIRLEEFERAAQLR
                     DKIRSMEQGGEQ"
     gene            complement(6150..6647)
                     /locus_tag="PLG49_09530"
     CDS             complement(6150..6647)
                     /locus_tag="PLG49_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17584.1"
                     /translation="MRNLKKILKDMETATLYIGKKTKDSLDNIKLKKQLYDESKKLSR
                     LYEALGKISFDIHSGRMHPASESNSLCSQIKEQIQLIEQLKNKLNRPIEEIENEETKD
                     SFLDNLENLKEEPVENEHLDAEALKPELGEDGFLLLKFCPNCQTGNHPESKKCIACGQ
                     VLNGV"
     gene            complement(6659..7165)
                     /locus_tag="PLG49_09535"
     CDS             complement(6659..7165)
                     /locus_tag="PLG49_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17585.1"
                     /translation="MPMDDWRMKLAKALKTVKDGTGELYQTTKINVDLGREQDNLKKI
                     YYDIGKKVHEIYQYGGSLGKFFDEKYLEIKEVEEKIEALQKKMEELKKVRVCIHCGKE
                     VESKAKFCPKCGSPMDDTVKEEKNTKETIKEEENRSPQLNIKVCPNCKAENQKDDKFC
                     LSCGRTLV"
BASE COUNT         2044 a         1590 c         1008 g         2774 t
ORIGIN      
        1 aactggaccg ggtaaaaatg ctgggaaatt ttaatttaag ataataaaga actaaccggg
       61 gttagttctt tattattatt tctaatgctt cattaatatt ttccacgcca taaatctgaa
      121 tgccatctaa ttttttcatg ttcttcagat ttgctttagg tataacacaa cttttaaatc
      181 ccattttcgc acattctgct actcttttct ctgccatact tacgcctctt atttcaccgg
      241 ttaggcctat ttctccaaac acgaccattt gggaatcgat aattttgtcc cgaaaacttg
      301 aagcaattgc agctgctatt cctaaatcaa gagcaggttc attaatttta atcccaccag
      361 ttaaattaac atagctgtca taactaatta actgcatacc tactctcttt tccaaaacag
      421 ccatgagcat aacgacacgg ttatagtcaa tacctgttgc agttcttctg ggcataccaa
      481 aattagtgaa gctgaccaaa gcctgaactt ccaccaataa tggtctggtc ccttccatac
      541 tgcaggaaac cactgagcct ggcgtattta caggtctgcc tgcaagcatt aattcagaag
      601 gattctcaac ttcaataaga cctttatcct gcatttcaaa gacgcctatt tcattggtag
      661 atccaaaacg gtttttaaca gctcttaaaa tacgataact ggcatgtctt tctccttcaa
      721 aatataaaac ggtatcaacc atatgttcca aaaccttcgg tccggcaata gcaccttctt
      781 ttgtcacatg gccaacaatg ataatagata tattatttcc ttttgatatt cgcattaagg
      841 aggaagtggc ttcccgaact tgactgacac tgccgggcgc tgaagatatt tcttctttat
      901 aaaccgtttg aatcgagtca atgataacga tatcagggct ttctttcatg ataatccctt
      961 ctatggcatc aatatttgtc tcagatagga tgagtaagtc atcagcccct aattccagcc
     1021 tgtctgcccg catttttatc tgtctgatgg attcttctcc ggaaacatat aaaattttgc
     1081 atccattttg accaatatga ctgcttattt gtaagagcaa agtagactta ccaatcccag
     1141 gatctccccc tactaaaatt agagaaccct taacgattcc tcctccaaga accctgtcca
     1201 gttctttact ccctgtaagt attctttcct cttcatctgt gccaatatct tttaaaataa
     1261 cgggatagct gtggggttct ttgccggata agacagaagg ttttctggaa tcagtaattt
     1321 cttcaacaaa agtactccag ccattacatg ccggacattt tcctaaccat tttgcagatt
     1381 catatccgca ttcctgacat acaaaaatat tttttgtttt cgccatacta tcttcctttt
     1441 tattattaaa gcacatctaa accagatgtg ctttaatgat tatcatattt tttcaatcct
     1501 gacattttgc tttcctgtag taaaatctat gctaaatgtc actttaccag ctaagcttgc
     1561 cttgactttc cccacctgaa cattgtccac gtatatttta tattcttttt caggctctaa
     1621 ctccatagta atctgagaag tgttttctcc ttccacagca aaagtaactt gcttttcatt
     1681 catggtaaaa ttatgcaccg ttgttccggg aacggattca tacaggagta ttccattctt
     1741 ttcaagcttc gtgattttat tatatgtttt gactttatac aggtccccat ccacttcaaa
     1801 gtcaatgact ttctttttag tgtccattaa atagtttccg aaacttaaac tattatcatc
     1861 ttccaatcgt attaattctt caacgactgc cacattggta accccctaga ctatttttat
     1921 aattttatta tactctattt cattcataaa ttccattcta aaatggtttt tttaaaaacc
     1981 ttgtcgtaat atctgctaaa gtcagattct aataaatttt aattgatttt cttctaaatc
     2041 aatttttata tgatctcctt ctttaatttt accatctaaa atttcttcag ccattttgtc
     2101 ttctatttta gactgaattg ctcttcttag aggtcttgca ccataggcct ggtcaaagcc
     2161 ttcttttgct aaatattctt ttgcactttc tgtaatctca atgctaatac tcatattttg
     2221 cttaatgcgt tttgctaatt gttcaaccat cacactgaca atctgcttaa tatggtcctg
     2281 attaagtgga tggaagacaa tcatttcatc aattctgttt aaaaattcag gtctgaaaag
     2341 ttttttaact tcgtccatca catttttctt catatcttca taattttttg tttcgttttc
     2401 aacagatgca aaacctaatt ttttaggctc gataatattt cttgccccga tattggaggt
     2461 cataatgaca acagtattct taaagtctac tcttcttccc tgagaatccg taatatgtcc
     2521 gtcgtctaaa acttgaagaa gaatattaaa aacatctgga tgagcctttt ctacttcatc
     2581 aaatagtata acagaatagg gttttcttct gattttttca cttaattgac ctccttcatc
     2641 atagccaaca tatccaggag gtgaaccaat aagtttagaa acactgtgtt tttccatata
     2701 ttcagacata tctattctaa ccatagcatt ttcatcacca aaaagggctt ctgctaaagc
     2761 ctttgtcagc tcagttttcc caacacctgt tggtcccagg aacaagaagg aaccaatggg
     2821 tcttttggga tcctttaagc ctaccctgcc tcttcttatg gcttttgcta cggcacgtac
     2881 agcctcttct tgtccaataa ctcttttatg aagtatttct tctaagtttt tcaatctttc
     2941 actttcttct tcggccagct ttttaaccgg aatacccgtc cagcttgaaa caatatccgc
     3001 aatttcttct tctgttacct cctgcttaga tttatcataa gcatctttcc agtcattctt
     3061 ttcctgttct aaaagtttct ttatttcatc ttgtttcttt ttaatttcgc cagctttttc
     3121 aaattcctcc gtcttaatgg cagcttcttt ttctttttcc aattcttcta atcttgcttc
     3181 aagttctttt acattaggtg gtgctgtata taaacgcagt ctaaccttgg aagctgcttc
     3241 atcaattaag tcaatggctt tgtctggtaa aaacctgtcg gtaatgtatc tgctggacaa
     3301 ttttaccgaa gcaacaatag catcgtcagt aatatgaacc tggtgatggg cttcatattt
     3361 atctcttaag ccctttaaga tttctatggt ttcttcttct gtaggttctt ctactttaac
     3421 aggctggaaa cgtcgttcaa gagcagcatc tttttcaata tattttcggt attcatccag
     3481 ggtagttgca ccaatgactt gaatttctcc tcttgctaaa gaaggtttaa gaatatttga
     3541 agcatcgatt gccccttctg ctgcacctgc acctataatg gtatgaagct cgtcaataaa
     3601 aagtatgaca tttccggcag tacggatttc atctaaagct ttctttatcc tttcttcgaa
     3661 ttctcctcta tatttggagc ccgcaatcat agcagataaa tcaagagata cgaccctttt
     3721 atttctgatg atttcaggca catttccttc tacaattttt tgtgccagtc cttctgcaat
     3781 agctgtttta ccaacacctg gttctcctac taaacatgga ttattttttg ttcttctgct
     3841 aagaatctga ataacccttt caatttcttt atctcttcca atgatcggat caaatttttg
     3901 ttctgtagcc attgcggtta aatcacggct aaattgatcc aaggtagggg tgtcgctgtg
     3961 accattttta gcgccttttt gattaatggg cataccacct tgtcctttat tattttcccc
     4021 taacatcgcc atgatgtcat cataaatttt ttgaggatta acccccatac tcatcaacaa
     4081 tttcacagca atacaatctt gttctcttag aagcgctaaa agcaaatgtt cggttccaat
     4141 ataacctgca cccattttta gtgcttccct gaagctcatt tctataatcc ttttagccct
     4201 tggagtaaaa tcctggggct ctgctgtagc tatatccccc atgccaataa cagcttttaa
     4261 tttatcaaca atcgcctctt cggatacacc ttgatttttt aatgctctgg cagcaacact
     4321 gtcatctatt cgaagcaatc ctatgagtat atgctccgta cctacatagc catgtcctaa
     4381 ttctgctgca gcttgttgag aagcttgaag cgcttcctga gcacgtcgtg taaatttccc
     4441 catcatcgta gattcctcct tttcttctaa tgtgattaaa actgctgtct aataaatgaa
     4501 gcacgttcta tatcgatttc ctgaccagtt aaactgatac ccatcttttt ttgtaaattt
     4561 gctggttgaa tatgcataac caaactataa agattcattt ttggttttgg ctcgtctaat
     4621 atacccattt catagccaat tttcacatcc gataaatagg tcatggcttc ttttgttgat
     4681 aaaacttttg catatttaac aatgccgtat gatctataaa ttttatccaa gaattccatt
     4741 tttctgtctt ttataatgtt ttctctgata tttttttcct gttctacgat ctgcatcgtc
     4801 actgtattta agttttcaat gatttctttt tcagactgtc ctaaagtaat ttgatttgaa
     4861 atttgatagt aacttccttc cgcctttgtt ccttcaccgt ataaacctct tacggcaaaa
     4921 cccaatttgc cgatggcctg aatgatattc tgaagctgac cagaccattc caaagcagga
     4981 aggtgaagca tataggatgc cctcatacct gttcctacat tagtcggaca ggaagttaag
     5041 taacctaatg tttcatcaaa cgcatattct atcgattctt ccagaacatc atcaatttta
     5101 tctgctaaat cccaggcttt atccaaattc attcctgctg ccatggattg tatcctgaga
     5161 tggtcttctt cattaaccat aatacttatg gattcatctc tttttagcaa aacaccacac
     5221 ggagcgtttt ttcttaccag ttcaggactt attatatgac tttccatcat agccctttta
     5281 ttaacaggac tgttgttata aacagaaata tattcaaatt ctttagataa aatcgtattc
     5341 ccttctaaca acactttttt agtttcttca atcaattttt ctgcttgagg cagagacaat
     5401 tttacagcaa aaggatattt tttgtagttt cttgccaatc ggatcctgca agaaaccacg
     5461 atatcagaat ctaaactatc ctcataccat tttctcattg ttctcccccc tgttccatgc
     5521 tgcggatttt gtcccgtaat tgggctgccc tttcaaattc ctccagtctg atggcttctt
     5581 tcaattcttt cttaagcatt tcaagttctc tttttatttt caattcgcct gccgcactac
     5641 ttggcaactt tccaacatgt tcattgttgc catggacgtt ttttataata ggcatcagac
     5701 ggctgctaaa agctgtataa cactgtgcgc agccaaaacg acctatctta gaaaattcat
     5761 cataagtcat cttacaggaa ggacattgta aagtcgtacc ctgactgcta tattctttcg
     5821 aggggacctt acccattaaa ggcatatcta aaaaacccgt taaaaaatcc tgaaaagaaa
     5881 tcggggtatc aaaatgtatg gactcagttt ctttagcaca ctgttcacat aaataaagtt
     5941 caactttttc attattattg atttgtatca catgcacatt agctatattt tttttacaac
     6001 gttcacataa cataatatcc cccttttcta tatggaatct ttatctagta tagcatgtac
     6061 tgttgagtca taatcttatt catcaaacat tatatgcatt gaataatttt ttagtaaaaa
     6121 acaagaccgt aaaaggtcct ttaaattaat tatactccat tcaatacttg tccacaagca
     6181 atgcactttt ttgattctgg atgattaccc gtttgacaat tggggcaaaa cttgagtaat
     6241 aaaaaaccat cttctcctaa ttcaggcttc agtgcctctg catctaaatg ttcattctcc
     6301 acaggctctt cctttaaatt ctccaaatta tccaaaaagc tgtctttggt ttcttcattt
     6361 tctatctctt ctatgggtct atttaattta ttttttagct gttctattaa ttgaatttgt
     6421 tctttaatct gactacaaag gctattactt tcacttgccg ggtgcattct tccactatgt
     6481 atgtcaaagg atatcttccc aagtgcctca tataatctac ttagtttttt tgattcgtca
     6541 tataattgct tttttaattt tatgttatct aaactgtctt tggttttttt ccctatataa
     6601 agggtggctg tttccatatc ttttaagatt ttttttaagt tcctcatagt tttctccttt
     6661 acactaaagt tctgccacat gataaacaaa acttatcatc tttttgattt tctgccttac
     6721 aattcggaca aacttttatg ttaagctgag gtgacctgtt ttcctcttct ttaatcgtct
     6781 ctttagtgtt tttctcctct tttactgtat catccatagg agaaccacat ttaggacaaa
     6841 attttgcttt actttcaact tcttttccac aatgaataca aaccctgacc ttctttaatt
     6901 cttccatttt cttttgcaat gcttctattt tttcttcaac ttctttgatt tctaaatact
     6961 tttcatcaaa gaatttacct aacgaacctc cgtattggta aatctcatga acctttttcc
     7021 ctatatcata gtaaatcttt tttaaattat cttgttctct ccccaaatct acattaattt
     7081 tagtcgtttg atacagctct cctgtaccat cctttacagt ctttaatgct ttggccagtt
     7141 tcattctcca atcatccata ggcatatttt tcaccttctt aattataata taaaaatatg
     7201 tataaataat gaattaatta ttactaattt tattaatttc attctagcat attgttaatt
     7261 gttggtaaat agttcatatt taattctatt atcaattaat atttattatt aattatataa
     7321 tattatatca taataatcaa atatttcgaa tcaaaattaa aaatataaat aaaaaccata
     7381 ttacattatg taatacggtt tttatttaaa aaagtc
//
LOCUS       DAOSUD010000092         7339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5017_length_7339_cov_21.228720, whole genome shotgun sequence.
ACCESSION   DAOSUD010000092 DAOSUD010000000
VERSION     DAOSUD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5017_length_7339_cov_21.228720"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLG49_09540"
     CDS             <1..233
                     /locus_tag="PLG49_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ17586.1"
                     /translation="SKERSKLGAIQNRLEHTIKNLDTAAENLQASESRIRDVDMAKEM
                     MNYTKLNILSQAAQSMLAQANQNPQIVLQLLR"
     gene            341..2185
                     /locus_tag="PLG49_09545"
     CDS             341..2185
                     /locus_tag="PLG49_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ17587.1"
                     /translation="MSYSLYEKNIQEYMKRTGKNIRAYEKNSNAVLEKTKDNYYTVKI
                     NESGREVYIHSKYSPIKEAERFVEKLEDYNIKTVFIVYGMGLGYHILELIKYLHKENP
                     LIIIEPNEGVLSKAFSIYDWTPLLRRDKTYIFTGTNANALKLVLGEFIDISNVENLML
                     LPFSQYHKIYSVFYEKVIEEVKNYLSLFKISKNTKEHFWEQFNKNFFNNLHSIFYQRS
                     INDLKNQFKNVPAFIVSAGPSLDKNVKELKNVKDKGIIFSGGRTLPTLLNHNIEPHFV
                     VSMDPGIAAYHVLKDCADHKFPLITTVVSNDTVISEHQGDVFYISNSEYRGLVQYFTQ
                     RDMDILPLGASVANASLAIAVYLGCNPIIFLGQDLAYTDGKHHADSCGGTTLKEDDDL
                     IEVEGIDGNKVFTNSVWHTFLMWMEKFIIHNPNKTYIDATEGGAKIHGTDIMTLRQAI
                     EKYCLKNEFIQDKINTIIKAKKIGYQDEKLQRLLMNLKVDLEKVHKISSQGFKEAHQL
                     LNKENTDEKDIRRLNLFETQLNDLIKRNEAVADILLRIINDIDTNNEFIEKMNESKEE
                     KCRRLINRNVALHKKIEDTAFNINKLVQSVIDQDIEIIKIFQCSISFV"
     gene            2215..2571
                     /locus_tag="PLG49_09550"
     CDS             2215..2571
                     /locus_tag="PLG49_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17588.1"
                     /translation="MNQRAEKMEVIQTLVEYTGRLINGINEAVDSFHEGMEGKGMDLV
                     VQIIDGLKWTMEAITLTLDAQVEPVDVSEIIEPLNAMIEALENIDYVLIGDVLEYEIL
                     PKVEEWNQKLNLTIGE"
     gene            2574..3995
                     /locus_tag="PLG49_09555"
     CDS             2574..3995
                     /locus_tag="PLG49_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ17589.1"
                     /translation="MDFCKINMDLLKVKDVDLYHLMNQFILKEEKYKKELTHSKVYTL
                     KISEYDYTRKKHVDFYLHSKYDPVREAEKFAKGQYSLEKEKIIIFGFGLGYHVEAIFS
                     LLKDHQKLYIFEMNLNVFKSALECRNLSNILSSDRVHLIISDHETQQALHLGNLLKED
                     AHLIIHPPSLKTIPKNSEKIRFILEDWNITKSETNEWREMLDENYRRNESLNAPNVGE
                     LFNQYKGLPFIIVSAGPSLEKNIHLLSDLEGRAFIFAVGRTLKTLLWENIEPDMFCII
                     DPQYEPTYDQIKGYENLDIPMVFFESASADTVSRYKGPKYFASNKKEHIENPAHIMDL
                     GGSVATAVLDLSIRFGGNPIVFVGQDLAFTDGQSHAYGNTVQENANRRKVKGQNGEWL
                     DTTLGLLSFKHWIENKIKDHPHIEFINATEGGAYIEGCRHMPLQDFIEEYIDYSKPAD
                     KRLRIRKLKEEIKIFTATIEFMN"
     gene            4007..5779
                     /locus_tag="PLG49_09560"
     CDS             4007..5779
                     /locus_tag="PLG49_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ17590.1"
                     /translation="MTIQIETSKSGVPTAKLQIGEKELYLHSKYDPVKEAVRNIGSLE
                     GYGLNTVFILFGLGLGYNLYELSKYARENLIIILEPNEELYLYSKELEHIKPIIENEN
                     VISLAGNACFNIYEILKQNTSIENYNHFKLYTLPYYDKIYADFYSQLLSIIHSYNLEL
                     QVQANAIEKLSSQISRNLAENLPYIIEGYSIISFKEIFKDCPAVIVSAGPSLEKNIDE
                     LKDVQDKAVIFAGVRTLKPLLDRGIIPHFLCNIDPQDITYELAKDHMDLSIPVISLVH
                     GNHILVEEWKGKKIFIPDDKLKNLVEYIFGERQDTIEVGGSVANSSTAIATYMGCNPL
                     ILVGQDLAFTDQKHHSDIASKVCEEDQKESSGDLLVEDIYGNRIPTSSQLYYYLNWFE
                     NYIKDHSNNTFIDATEGGAKIKGTKIMTLKDTIIKYCDQHINPKDKIKLIMENKENNV
                     YYKGIEKLKNVLKELRVIKKCSKSNLEWSLELKRYYEGKSREKFNKILYQLDRNDEAI
                     NKAVLANELLFYYKQPGLLQIAQEFEPKIIEKEENAYQRILEKNEKLYKLQYNAAVHF
                     EKTIETLIEENEIKLLTAFIKIQS"
     gene            5797..6798
                     /locus_tag="PLG49_09565"
     CDS             5797..6798
                     /locus_tag="PLG49_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOQ17591.1"
                     /translation="MFENKKILVLGGTGTIGQALVKKLIEYNPQVIRVFSRDEYKQFV
                     MEQELGKRKNIRFLLGDIRDKERLERAMHGIDIVFYLAALKHVPACEYNPFEAVKTNV
                     LGTQNVIECAINQKVERVIYTSSDKAISPTNTMGATKLLAERLMSSADYYKGGKRPIF
                     AAVRFGNVMGSRGSVIPLFKQQILENKKITLTDPNMSRFMMSITQAVGLVLKATEYAQ
                     GGDIFVLKMPVINLKDLAEVVIEETRKKYNLPWDEEIKIETIGLRPGEKMYEELMTKE
                     EAMRAEELDEMFRIYPFVQSYPKVNLNNISEYSTDSSKAKALSKEELRELILSENLI"
     gene            6921..>7339
                     /locus_tag="PLG49_09570"
     CDS             6921..>7339
                     /locus_tag="PLG49_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ17592.1"
                     /translation="MHRIAIIGSGYVGLVTGACLADFGMKVICVDINEDKINQLNQGK
                     VPIYEPGLEQLIERNTYYKRLTFTTDIKEAVNQSDVIFIAVGTPPNEDGSADLTHVLK
                     AAEDIASHMNEYKVIVDKSTVPVGTGQKVKQTVKKIL"
BASE COUNT         2769 a         1039 c         1379 g         2152 t
ORIGIN      
        1 tatctaaaga aagatccaag cttggtgcta ttcaaaaccg cttagaacat accatcaaga
       61 atttggatac agctgctgaa aacttacaag catctgaatc cagaattcgt gacgttgata
      121 tggctaagga aatgatgaac tacaccaaac ttaacatctt aagccaagct gctcaatcca
      181 tgcttgcaca agcaaatcaa aatccacaaa ttgtacttca attattaaga taacaaaatt
      241 ttcaaaaagc caaggcatat gccttggctt ttttctatta atgatttgtg cttttttgcc
      301 gatataaatt gtacagtatt ttatctaaag gagctttaag atgagttatt ccctttatga
      361 gaaaaacata caagaataca tgaaaagaac aggaaagaat atcagagcat atgagaaaaa
      421 ttccaatgca gttttagaga aaactaagga caattattat acagttaaaa taaatgaatc
      481 aggaagagaa gtttatattc acagcaaata ctctccgatt aaagaagcag aacgatttgt
      541 ggagaagtta gaagattaca atataaagac agtttttata gtatatggga tgggattagg
      601 gtatcatatc cttgaactaa taaagtactt acacaaggaa aaccctttaa tcattattga
      661 acctaatgaa ggggtactct ctaaagcatt ttctatttat gactggactc ctcttttgag
      721 aagagataag acttatattt ttacaggtac taatgccaat gcactgaaac ttgtattagg
      781 agaatttatt gatatttcta atgtagagaa tttaatgttg cttccctttt ctcaatatca
      841 taaaatttat tctgtatttt atgaaaaggt tatagaagaa gttaaaaact atctttcttt
      901 gtttaaaatt tctaaaaata cgaaagaaca cttttgggaa caatttaata aaaatttctt
      961 taataattta cactctatat tttatcaacg gtcaattaat gatttaaaaa accaatttaa
     1021 aaatgttcct gcatttatcg tatcagcagg gccatctttg gataaaaatg taaaagagct
     1081 taaaaatgta aaagacaaag gcattatttt ttcaggaggc agaactttgc ctactttgct
     1141 taatcataat attgagcctc actttgtagt ttctatggat cctggaatag cagcttatca
     1201 tgttctgaaa gactgtgcag atcacaaatt tcctcttatt actacggtgg tttcaaatga
     1261 tacagtgatt tctgaacacc agggagatgt attttatatc tcaaatagtg aatatagagg
     1321 gctagttcaa tattttacgc aaagagatat ggatatttta cctctggggg cttctgtagc
     1381 caatgcttct ttggctattg cagtttattt aggttgtaat cccattatat ttttaggaca
     1441 ggacttggca tatacagatg ggaaacatca tgcggatagt tgtggaggta ccacacttaa
     1501 ggaggatgat gatctcattg aggttgaagg aattgacggc aataaggtat tcaccaatag
     1561 cgtatggcat acttttttaa tgtggatgga aaaatttata atccataatc cgaataaaac
     1621 ttatatagat gccacagaag gtggagccaa aattcatgga acggatatta tgactttaag
     1681 acaagctatt gagaaatact gtcttaaaaa tgagtttata caggataaaa ttaatactat
     1741 tattaaagct aaaaaaatag gatatcaaga cgaaaaactc caacgccttt taatgaattt
     1801 aaaagtagat ttagaaaagg ttcataaaat ttctagccaa ggatttaaag aagcacatca
     1861 attactcaat aaagaaaata cagatgagaa agatatcaga aggcttaatc tgtttgaaac
     1921 acaacttaat gacctgataa aacgcaatga ggctgttgca gatattttat taagaataat
     1981 aaatgatatt gacacaaata atgaattcat agaaaaaatg aatgaaagca aagaagagaa
     2041 atgtcgtcgt ttaattaata gaaatgtagc acttcataaa aaaatagaag ataccgcttt
     2101 taatatcaat aaactggtac aaagtgttat tgatcaagac atagagatta ttaaaatatt
     2161 tcagtgcagt atttcttttg tttaaaaaaa taattttttg aaaaggggat aattatgaat
     2221 caaagagcag aaaaaatgga agttattcaa acattagtcg aatacactgg cagacttatc
     2281 aatggaatca atgaagcagt agactccttt catgaaggaa tggaaggcaa aggtatggat
     2341 ctggttgttc aaattatcga tgggttaaaa tggactatgg aagctattac ccttaccctg
     2401 gatgcacagg tggagcccgt tgatgtttca gaaatcattg aacctttaaa tgcaatgatt
     2461 gaagctttag aaaacataga ctatgtatta attggagacg tattggaata tgaaattctg
     2521 cctaaagttg aagagtggaa tcaaaaactt aatttaacta taggggaata aatatggatt
     2581 tttgcaaaat caatatggat ttactcaaag ttaaagatgt agatctctat catctcatga
     2641 accaatttat attgaaagaa gaaaagtaca agaaagaact aacccattct aaggtatata
     2701 cccttaaaat ttctgagtat gattatacaa gaaaaaagca tgtagatttt tatttgcata
     2761 gtaaatatga tcccgttaga gaagcagaga aattcgcaaa aggtcaatac agtttagaaa
     2821 aagaaaaaat cattattttc ggattcggcc tgggttatca tgttgaagca atattcagtc
     2881 tgttaaaaga tcatcaaaaa ctgtatatat tcgaaatgaa cctgaacgta tttaaatctg
     2941 ctttagaatg caggaattta tcaaatattt tatccagtga tcgagtacat cttataatct
     3001 ctgatcatga gacacaacag gcattgcatc tagggaattt attgaaagaa gatgcccatc
     3061 tcattatcca ccctccctct ttaaagacaa tacctaaaaa tagcgagaaa atccgattta
     3121 ttttagagga ctggaatatt accaagagtg aaaccaatga gtggagagaa atgctggatg
     3181 aaaactatag acgaaatgaa tccttaaatg ctcctaatgt gggggaatta tttaatcaat
     3241 ataaagggtt gccttttatc attgtatcgg cagggccttc attagaaaag aatattcatt
     3301 tgctcagcga tttggaaggc agggcgttta tctttgcagt aggaagaact ctaaagactt
     3361 tactctggga aaacatagaa ccagatatgt tttgcatcat agatcctcaa tatgaaccta
     3421 cttacgatca aatcaaagga tatgaaaatc tggatatccc tatggtattt tttgaatcag
     3481 ccagtgcaga tactgtatca agatataaag ggcctaaata ttttgcttct aataaaaaag
     3541 aacatataga gaatccggcg cacattatgg acttaggagg atctgttgct accgcagtac
     3601 tcgatttgtc tattcgattc ggaggaaacc ctattgtgtt tgtaggacaa gacttggctt
     3661 ttacggatgg acaaagtcat gcttatggaa atacggtcca agaaaatgct aacagaagga
     3721 aagtcaaagg gcaaaatgga gaatggctgg atacgacttt gggactactg agttttaagc
     3781 attggataga gaataaaatc aaggatcatc ctcacatcga atttattaat gctactgaag
     3841 gtggagccta tatagaggga tgcagacata tgcctctgca ggattttatt gaagagtata
     3901 tagattactc aaaacctgca gacaaaagat taaggataag aaagctaaag gaagaaataa
     3961 aaatctttac agctaccatt gagtttatga attagaaggt gtaacgatga ctatccaaat
     4021 agaaacttca aaaagtggag ttccaactgc aaaactccaa ataggggaaa aggaacttta
     4081 tttgcacagc aaatacgatc ctgtaaaaga agcagtgcgc aatataggct cattagaggg
     4141 atatggatta aatactgtat ttatattatt tggattagga ttaggatata atttgtatga
     4201 attatcaaaa tatgcaaggg aaaatcttat tattatttta gagcccaatg aagaattata
     4261 tttatattca aaagagctgg aacatataaa acctattata gagaacgaaa atgttatttc
     4321 tcttgcaggc aatgcatgtt ttaacattta tgaaatctta aagcaaaata cctctataga
     4381 aaattataat cattttaagc tgtatacact accttactat gataagatat atgctgattt
     4441 ttatagccag cttttaagta ttattcatag ctataatctt gaacttcaag tacaagcgaa
     4501 tgctatcgaa aagctctcaa gccaaattag taggaatttg gcagaaaatc ttccatatat
     4561 catagaaggc tattctatta tttcttttaa agagattttt aaagattgcc ctgcggtgat
     4621 cgtatcggca ggaccttccc tggaaaaaaa tattgatgag cttaaagatg tacaggataa
     4681 agcagtgatt tttgcgggag taagaacatt aaaaccctta ttagataggg gaattattcc
     4741 tcattttttg tgtaatattg atcctcaaga tattacctat gaattagcca aggaccatat
     4801 ggatttatcc ataccagtta tttctttagt tcatgggaat catattcttg tcgaagaatg
     4861 gaaaggcaaa aaaatattca taccggatga caagcttaaa aaccttgtag aatatatatt
     4921 cggtgaacga caagatacta tagaagtagg agggtctgtt gccaacagct ctactgctat
     4981 cgccacatac atgggatgta atcctctcat tttagtaggg caggatttgg catttaccga
     5041 tcaaaaacac catagcgaca tagcatctaa agtatgcgaa gaagatcaaa aagaatcatc
     5101 aggggatcta ttggtagaag atatttatgg aaataggatt cctaccagtt cgcaactgta
     5161 ttattatctt aattggtttg aaaactatat caaagatcat tccaataata cttttataga
     5221 tgccacagaa ggcggagcta aaatcaaagg caccaagatc atgacattaa aagatactat
     5281 tataaaatac tgtgatcagc atattaatcc aaaagacaag ataaaactta taatggaaaa
     5341 caaagagaat aatgtatatt acaagggaat agaaaaactg aaaaatgtcc taaaggaatt
     5401 aagagtgata aaaaagtgct ctaaatcaaa tttggagtgg tctttagagc ttaagagata
     5461 ttatgaagga aaaagcagag aaaaatttaa taagatatta taccaattag acaggaatga
     5521 tgaagcaatc aacaaggctg ttcttgccaa tgagctttta ttttactata agcagcccgg
     5581 gttattacag attgctcagg aatttgaacc taaaataatc gaaaaagaag aaaacgctta
     5641 tcagcgtatt ttagaaaaga atgaaaagtt atataagctt caatataatg cagcggtaca
     5701 ctttgaaaaa accatagaaa cactgataga agaaaatgag atcaaactac ttacagcatt
     5761 tattaaaatt caatcttagg ttaagtgggt ggaataatgt ttgaaaacaa aaagatttta
     5821 gtccttgggg ggacaggaac gattggacaa gcgttagtaa aaaaactcat agagtataat
     5881 ccacaggtta ttcgagtatt tagcagggat gagtataaac agtttgtcat ggaacaagaa
     5941 ctgggaaagc gtaagaatat caggtttttg ttgggagaca taagagataa ggaaagattg
     6001 gaacgggcaa tgcatgggat tgatattgta ttttacttag ctgccctaaa acatgttcca
     6061 gcctgtgaat acaatccttt tgaagcggtt aaaactaatg ttctcggtac tcagaatgtc
     6121 atagagtgtg cgattaatca aaaagtagaa agagtcattt atacaagcag tgataaagcc
     6181 atcagcccaa ctaacaccat gggagctacc aagcttttag cagagaggtt gatgtcttct
     6241 gctgattatt ataaaggtgg caaaagaccc atatttgcgg cggtgcgttt tggaaacgtc
     6301 atgggatcca gaggatcggt gatccctctt tttaaacagc aaatcttaga aaacaaaaaa
     6361 ataacgctta cagatcctaa tatgtcaaga tttatgatga gtattactca ggcggtgggt
     6421 ttggtgctaa aagctacgga gtatgctcag ggaggagata ttttcgtcct taaaatgccg
     6481 gttatcaact taaaggattt agcagaagtt gttattgaag aaacaagaaa aaaatacaac
     6541 ttaccttggg atgaagaaat taagattgaa accataggct taagaccagg agaaaaaatg
     6601 tacgaagagc ttatgaccaa agaagaagct atgagagccg aagaactgga cgaaatgttt
     6661 agaatttatc cgtttgtgca aagttatcct aaggttaatc ttaataatat cagtgaatat
     6721 tcaacagatt cttcaaaagc aaaagcttta agcaaagaag aattaagaga acttatttta
     6781 agtgaaaatt taatataaat cattataaca gtacgaaaaa gaactgtttt ttattatcta
     6841 cagatttttc taaggttttt gtaaagttat gacgaaaata ataatagtgt atatttatta
     6901 atacagaaaa aggtgataac atgcatcgta ttgcgataat tggttcagga tatgtaggat
     6961 tagtaacagg agcttgtctt gcagactttg gaatgaaagt gatctgtgta gatatcaatg
     7021 aagataaaat taatcaactc aaccaaggga aggtacctat ctatgaacct ggacttgaac
     7081 aattaattga gagaaataca tattataaac gattaacatt tacaacggat ataaaagaag
     7141 cagtgaatca aagtgatgtg attttcattg cagtaggaac gcctccaaat gaagacggca
     7201 gtgcagatct aactcatgta ttaaaggcag cagaagatat tgctagtcat atgaacgaat
     7261 ataaagtaat cgtcgataaa tctacagtac ctgttggaac tgggcaaaag gtcaaacaaa
     7321 ctgtgaaaaa aatattaga
//
LOCUS       DAOSUD010000093         7310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5043_length_7310_cov_24.527498, whole genome shotgun sequence.
ACCESSION   DAOSUD010000093 DAOSUD010000000
VERSION     DAOSUD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_7310_cov_24.527498"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..695
                     /locus_tag="PLG49_09575"
     CDS             234..695
                     /locus_tag="PLG49_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17593.1"
                     /translation="MNQNFSGYLKLFSESENQKILVKGTWGLEKESQRINLSGELALT
                     PHPEAFGDKLSNPYITTDFSESQLEFITPPFASIEQSYDFLLNLHNKAGKALKNELIW
                     PLSMPPRLPDEEKIPIAKFNNSPKGKEQEIYRKGLALRYGKKDADDLRHSL"
     gene            655..1689
                     /locus_tag="PLG49_09580"
     CDS             655..1689
                     /locus_tag="PLG49_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17594.1"
                     /translation="MAKKMQMICGIHYNFSLADEFLDLLYNQQSNKISEQEFINQLYF
                     SLARNFLRYKWLLIYLLGASPSFDSSYKVLAKESKFIKEVIPKELHFISNFKKYATSL
                     RVSPFGYSSKIQGKNHISYNSLEEYIRGLRQLLNTPCKEYLDLGLLANGEPLQLNANI
                     LQKESELYSPIRFKQQLHEGENSLDALEKRGIRYIEIRVLDLNPFEKTGLSLSQLYFL
                     HVFILFCLFEENRPISKEDSEIININQQLVAFAGRKTDLMLYDDKRKKQSLHELGNKI
                     FDKLESIAFLMDQGINDHKYRQSVQKEKEKLYDISLLPSSLIENEMKNKQESFIEFGL
                     RWAKKHALEG"
     gene            1691..2680
                     /gene="gshAB"
                     /locus_tag="PLG49_09585"
     CDS             1691..2680
                     /gene="gshAB"
                     /locus_tag="PLG49_09585"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HOQ17595.1"
                     /translation="MISKDYKSLELSTQLIIKEALKRKIEVEVLDWEDNFIRLKKGDK
                     VEYIKQATRTSADTYIAPLIMENKEVTKIILKEYGLSVPESLTIRSVEDAMAAYPKFV
                     TKDIVVKPKSTNFGKGITILKKLNSQKEFLDAVQYAFKEDKTLLIEDFIEGKEYRFLV
                     IGDEVPAILHRVPANVKGDGVHSIRELVKIKNQDPLRGKNYVTPLEKINLGHVEEEFL
                     KAQGKDFDYIPKENETVYLRKNSNISTGGDSIDYTDEISDAYKKIAIKAAQAVGAKIC
                     GADIIIKNLNEKPSKDNYSIIELNFNPALHIHDYPYIGKNREVEKKVLDLLKF"
     gene            complement(2713..3807)
                     /gene="mnmA"
                     /locus_tag="PLG49_09590"
     CDS             complement(2713..3807)
                     /gene="mnmA"
                     /locus_tag="PLG49_09590"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ17596.1"
                     /translation="MSKKKVVVGMSGGVDSSVTAYLLKEQGYEVIGMTMQIWQDETRE
                     EMENHGGCCGLSAVDDARRVCQKIGIPHYVVNFKEVFSNKVIDYFAQEYFKGKTPNPC
                     IACNRYIKWESMLFKALQLEAEYIATGHYAKIVYLPERKRYTLKESAALGKDQTYVLY
                     NLTQEQLKHTLMPLGDYNKDQVREIAKKAGLIVASKPDSQEICFIPDNDYGKFLEENF
                     DMPIVPGNFVDTQGHVLGKHKGIIYYTVGQRKGLGIALGKPMYVVEIRPETNEVVLGE
                     HNEVFKKVLYANELNFMAIEKLEGKMRLTGKIRYSHAKVPCTIEMAGDDLIRCEFDEA
                     QRAITPGQAVVFYDGDIVVGGGTILYGVNE"
     gene            complement(3825..4265)
                     /gene="nifU"
                     /locus_tag="PLG49_09595"
     CDS             complement(3825..4265)
                     /gene="nifU"
                     /locus_tag="PLG49_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOQ17597.1"
                     /translation="MYSEKVMDHFMNPRNMGEIENASGVGVVGNPKCGDIMKIYLDIE
                     DGIIKDVKFKTFGCGAAVATSSMATELVKGKTIEEALKVTNKAVMEALDGLPPVKVHC
                     SVLAEEAIHAALWDYAQKNGIEIEGLKPPVDHDDHHHDIHEDDE"
     gene            complement(4305..5489)
                     /gene="nifS"
                     /locus_tag="PLG49_09600"
     CDS             complement(4305..5489)
                     /gene="nifS"
                     /locus_tag="PLG49_09600"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOQ17598.1"
                     /translation="MKMHKIYFDHAATTSTRKEVVDIMLPYFTESFGNPSSVYEIARK
                     NKQAVDEARQKTADLLGADFNEIYFTSGGTESDNWAIKGVAESYKSKGNHIITSAIEH
                     HAVLHTCQYLESKGYEVTYLPVDEYGQVNPEDVRKAIKDTTILISIMYANNEIGTIQP
                     IEEIGRIAKERGVLFHTDAVQAMGHIPIDVKKANVDLLSFSGHKFHGPKGIGALYIRK
                     GVRIKPLLHGGAQERNRRAGTENVPGIVGLAAALELAIRDMEENNKKMLELRDYIIRG
                     ITERIEHVKLNGHPTKRLPNNINFTFDFIEGESLLLLLDSKGIYCSSGSACTSGSLDP
                     SHVLLAIGLPHERAHGSLRITLGDENTKEEADYLLEVLPSVVQRLRDMSPLYEEFKRK
                     NK"
     gene            complement(5515..5964)
                     /locus_tag="PLG49_09605"
     CDS             complement(5515..5964)
                     /locus_tag="PLG49_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOQ17599.1"
                     /translation="MRLSTKGRYGLKAMVDLAIHSQDKQIALRHIAERQDISEHYLEQ
                     LIAVLRKSGLVKSVRGAQGGYSLALPPDEITVGDILRALEGSLAPVACVDDEFAENCA
                     EWDYCVTKFVWQKIRDKVNEVVDSITLQDLAEEHEKLNCDKEYMYYI"
     gene            6218..>7310
                     /locus_tag="PLG49_09610"
     CDS             6218..>7310
                     /locus_tag="PLG49_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOQ17600.1"
                     /translation="MKNLKLIATTSFGLEAIVKKELMDLGYTDLKVSDGKVTFMGGKE
                     DIVRTNLWLRSADRILLEMGEFKALTFEELFEKTKALPWDEWIPIDGKFTVTGKSVKS
                     KLYSVPDCQAIVKKAVVEKLKNKYQVEWFKESGAEYTIQVSLLKDLASLTIDTSGTGL
                     HKRGYRQKAVEAPLRETLAAALIQLSYWNKNRSLYDFFCGSGTIPIEAALIGKNIAPG
                     LNRNFACMDWPQIEKEIWKKEKTAAYKAIDQEVQLKITAYDIDPKAICIAKENAREAG
                     VDDCIRFEVKDAKKINIQEPYGILITNPPYGERLGEREEIKNLYKSLGKIFKDESTWS
                     LYFLTSVEEFEKYFGKKANKKRKLYNGRIK"
BASE COUNT         2467 a         1416 c         1147 g         2280 t
ORIGIN      
        1 tataatttat tagagtttat ttattattta tatacaaaaa tatttatatt attaaattat
       61 ttttgtcgtt ctttaagtca taatgaaaaa aattatcaat tacctcatta tttcattaat
      121 atataaaatg gtttaaataa tttactttac aatattaatc agttacttta tcatcaaata
      181 ataaaaatat gatatgataa taaataaaac tataaatata aaaaggtgaa tctatgaatc
      241 aaaatttttc tggatatctt aaattattct ctgaatcaga aaatcaaaaa atacttgtta
      301 aaggtacctg gggactcgaa aaagaatccc agagaataaa tctttccgga gaactggctc
      361 ttacacctca tccggaagct tttggtgaca agcttagcaa tccctatatt acaacagatt
      421 tttctgaaag tcagttagaa tttattacgc caccctttgc cagcatagaa caaagctatg
      481 actttctttt aaatcttcac aataaagctg gtaaagcatt aaaaaatgaa ttaatctggc
      541 ctcttagtat gcccccaaga cttcctgatg aagaaaaaat tcctatcgct aaatttaaca
      601 acagtccaaa aggaaaagaa caagaaatct acagaaaagg cttggcctta agatatggca
      661 aaaaagatgc agatgatttg cggcattcat tataactttt ctctggctga tgaatttctt
      721 gatttattat acaatcaaca atctaataaa atctctgagc aggaatttat caatcagttg
      781 tatttttctt tagcccggaa ctttctaaga tataaatggc ttttgatata tcttttggga
      841 gcatcacctt cctttgattc ttcttataaa gtattagcca aagaatcaaa atttataaaa
      901 gaggttattc caaaagaatt acattttatc agtaacttta aaaaatatgc tacgtccctt
      961 agagtaagtc cttttggtta ttccagcaaa attcagggaa aaaatcatat ttcatacaac
     1021 agtcttgaag aatatatcag aggcttgcgt caattattaa atacgccctg taaagaatat
     1081 ttggacctag ggctgcttgc aaacggggaa cctttacaat taaatgcgaa tatactgcaa
     1141 aaagaaagtg aattatattc acccattcgt ttcaagcaac aactccatga aggggaaaac
     1201 tctctggatg ccttggaaaa aagaggaatt cgctatattg aaataagagt tttagattta
     1261 aacccatttg aaaaaacagg tttaagttta agtcaattat attttcttca tgtgtttatc
     1321 cttttttgct tatttgaaga aaacagacct atttctaaag aagattcaga aataatcaat
     1381 ataaatcaac aattggtagc ttttgctggc agaaagacag atttaatgct atatgatgac
     1441 aagagaaaga aacaaagcct acatgagctt ggaaataaga tatttgataa actggaaagc
     1501 attgctttct taatggatca aggtataaat gaccataaat acagacaaag tgttcaaaaa
     1561 gaaaaagaaa agctttatga tatttcttta ctgccctcaa gtttaattga aaacgaaatg
     1621 aaaaacaaac aggaaagctt tattgagttt ggattaagat gggcaaaaaa acatgctttg
     1681 gagggatgaa atgatttcaa aagactataa aagcttagaa ctgtccacac aattaattat
     1741 caaagaagct ttaaaaagaa agatagaagt tgaagtcctg gattgggaag ataattttat
     1801 ccgcctgaaa aaaggggata aggttgaata tatcaaacag gccaccagga cctctgcaga
     1861 tacttatatt gccccattaa ttatggaaaa caaagaagta accaaaatca ttttaaaaga
     1921 gtatgggttg tctgtcccag aatctttgac catccgttct gtggaggatg ctatggctgc
     1981 atatcccaaa ttcgtgacca aagacattgt ggtaaaaccc aaatcaacca attttggtaa
     2041 aggcataacg attttaaaaa aactgaattc acaaaaagaa tttcttgatg cggttcagta
     2101 tgcttttaaa gaagataaga cacttttaat agaagatttt attgaaggaa aagaatatcg
     2161 ttttttagtg attggcgatg aagtgcctgc cattttacac cgggtccctg caaatgtaaa
     2221 aggcgatggc gtccacagca ttcgagaatt agtcaaaata aaaaatcagg atccattaag
     2281 gggaaaaaat tatgtaaccc cccttgaaaa gattaactta ggtcatgtag aagaagaatt
     2341 tttaaaagcc cagggaaaag attttgacta tatcccaaag gaaaatgaaa ctgtttatct
     2401 gcgtaaaaac tccaatatca gcactggagg cgacagtatc gattataccg atgaaatatc
     2461 tgatgcctat aaaaaaattg ccatcaaagc cgctcaagca gtgggtgcca aaatctgtgg
     2521 ggcagatatt atcattaaaa atcttaatga aaaaccttca aaggataatt atagtatcat
     2581 cgaacttaat tttaatcctg cccttcatat tcatgattac ccttatattg ggaaaaacag
     2641 agaagttgaa aagaaagtcc ttgatttatt aaaattttag cctataaaaa aatccttgga
     2701 tatccaagga ttttattcgt ttacaccata caaaatggtt cctcccccaa ccacaatatc
     2761 tccatcataa aagacgaccg cctgtccagg agtgattgct ctttgggctt catcaaattc
     2821 acaccttatt aaatcatccc ccgccatctc aatagtacaa ggtactttag catggctgta
     2881 gcggattttc cctgtcagtc tcatcttgcc ttccaacttt tcaatagcca taaagttcag
     2941 ctcatttgca tacaatacct ttttgaagac ttcattgtgt tcacctaaga ctacttcatt
     3001 tgtttcaggt cttatttcta caacatacat aggttttcca agggcaatgc caagcccttt
     3061 tctttgtccg accgtgtaat aaataatccc tttatgtttt ccaagaacat gaccttgagt
     3121 atctacaaaa ttaccaggga caattggcat atcaaaattt tcttctaaaa atttaccata
     3181 atcattatct ggaataaaac agatttcctg gctgtcaggc ttagatgcca caataagccc
     3241 tgccttcttt gcaatttccc tgacttgatc tttattatag tcccctaagg gcattaaagt
     3301 atgctttaac tgctcttgtg tcaggttata aaggacatag gtttggtcct tgcctaaagc
     3361 agctgattct ttcagggtat atcttttcct ttccggcaaa taaacaattt ttgcataatg
     3421 gcccgttgca atatattctg cttctaattg aagggcttta aacaacatag attcccattt
     3481 gatatagcgg ttacatgcta tgcaaggatt aggcgtttta cctttaaaat attcctgtgc
     3541 aaaataatca atgaccttat tactaaaaac ttctttgaaa ttcaccacat aatggggaat
     3601 acctattttt tgacatactc ttctggcatc gtctactgcg gacagaccac agcatccccc
     3661 atgattttcc atttcttctc ttgtttcatc ctgccaaatc tgcatggtca tacctataac
     3721 ctcataacct tgctccttaa gcaagtaagc agtaacggaa ctatcaacgc ctccagacat
     3781 ccctacaaca actttttttt tagacattct ttcacctcaa aaacttattc gtcatcctca
     3841 tgaatatcat gatgatgatc gtcgtggtca acaggaggct ttaatccttc aatttctatt
     3901 ccgttttttt gtgcataatc ccataatgct gcatgaattg cttcttctgc caaaacagag
     3961 caatgtacct ttacaggtgg caaaccgtca agagcttcca taaccgcttt attggttacc
     4021 tttaatgctt cttcaatagt cttgcctttt accagctctg ttgccatgct gctcgttgca
     4081 acagcagcac cacagccaaa ggttttaaat ttaacatctt taataatgcc atcttctata
     4141 tcaagatata ttttcataat atctccacat ttaggattgc caacaacccc tacacctgag
     4201 gcattttcaa tttcacccat atttctggga ttcataaaat gatccatgac tttttcgcta
     4261 tacataaata cacctcttct aattgattat tatctatttt ttacttattt gttttttctt
     4321 ttaaactcct catacaaagg agacatatct cttaaccttt gaacaacaga cggaagcact
     4381 tcaagcaaat aatccgcctc ttctttggta ttttcatctc caagagtaat tcttaaagaa
     4441 ccatgagctc tttcatgagg aagcccgatt gccagtaaaa catgagacgg atccagcgaa
     4501 ccggatgtac atgctgaacc actggaacaa taaatgcctt tactgtctaa taacaataat
     4561 aaggattcac cttcaataaa atcaaaagta aaattaatat tattgggcaa cctctttgta
     4621 ggatgaccat ttaacttcac gtgttcaatt ctttcagtaa tccctctgat aatataatcc
     4681 ctaagttcaa gcattttttt attattttct tccatatctc ttatagccag ttctaaagcc
     4741 gcagccaagc ctacaattcc aggaacattt tctgttcctg cccttctgtt cctttcctga
     4801 gcccctccat gtaaaagtgg cttaattctt acgccttttc ttatatagag agcaccaatt
     4861 cccttgggac catgaaattt atgtcccgaa aaagacaaca aatctacatt ggcttttttc
     4921 acatcgatcg gaatatgccc catggcctga accgcatccg tatggaataa aacacctctt
     4981 tcttttgcaa tacggccaat ttcttctata ggctgaatgg taccaatttc attattggca
     5041 tacataatag aaatcagaat ggtcgtgtct ttaatggcct ttcttacatc ttcaggatta
     5101 acttgtccat attcgtcaac aggtaaatac gtcacttcat agcctttaga ttctaaatac
     5161 tgacaagtat gaaggactgc atggtgctcg atagcagatg taatgatgtg atttcccttg
     5221 cttttataac tttctgctac gcctttaatg gcccagttgt ccgattcagt tcctccactg
     5281 gtaaaatata tctcattaaa atctgcccct aaaagatcgg cagtcttctg tctggcttcg
     5341 tcaactgctt gtttgttctt tcttgcaatt tcataaacac tggaaggatt cccaaaactt
     5401 tcggtaaaat atggcaacat aatatctaca acttcttttc tcgtagaagt tgtagcagca
     5461 tgatcaaaat atatcttatg cattttcaca ccactcctca cttgcaagct aaaactaaat
     5521 ataatacata tattctttgt cacaatttaa tttttcatgt tcttcagcca aatcctgaag
     5581 ggtgatagaa tcaacgactt cattgacctt atcccggatt ttttgccaaa caaatttagt
     5641 aacacaataa tcccactcag cgcagttttc agcaaactca tcatcaacac aagcaacagg
     5701 tgctaaagaa ccttccaatg ccctgagtat atctccaaca gtgatttcgt caggaggtaa
     5761 agccaggctg tagcctccct gtgcccctct gacactcttc actaaaccgg atttccttaa
     5821 aacagcaatt aactgctcta aataatgctc ggaaatgtct tgtctttccg caatatgcct
     5881 taaagcaatc tgcttatcct gactatgaat tgccaaatct accattgctt ttaaaccata
     5941 tctgccttta gttgataatc tcatactctc acctcgagat ttaattccta gtttattact
     6001 cggttttcca taatagaata tcactttatg atttgactgt caataagata ttatattttt
     6061 ctattacaat cgtcccaatc actgatttta tcataaataa aaacaaaaga atgcgtaaaa
     6121 ctaatgaatg tatcaattat tttaattcaa ttattttcat ttttcccaat atgcattaga
     6181 atattactgt ctaaactaaa cgataaggag tattcaaatg aaaaatttaa agttaattgc
     6241 tacgaccagc tttggattag aagccatcgt taaaaaagaa ttaatggatt taggctatac
     6301 agaccttaaa gtttctgacg gcaaagttac ttttatggga ggaaaagaag atattgtccg
     6361 caccaatctc tggttgcgct ctgccgaccg tatcttattg gaaatgggag aatttaaggc
     6421 cctgactttt gaagaacttt ttgaaaaaac caaagcccta ccctgggatg aatggattcc
     6481 catagatggt aagtttactg ttacgggtaa atccgttaaa tccaaactct acagcgttcc
     6541 agactgccag gcaattgtta aaaaagccgt tgttgaaaaa ttaaaaaaca aatatcaagt
     6601 ggaatggttt aaagaaagcg gcgctgaata tactatccag gtttccctgt taaaggacct
     6661 ggcaagcctt accattgata ccagtggtac tggccttcat aaaagagggt acagacaaaa
     6721 agctgtggaa gcccctttaa gagaaacatt agctgcagct ttaatacaat taagttattg
     6781 gaataaaaac agaagcctct atgatttctt ttgcggttct ggaacaatcc cgatagaagc
     6841 tgcccttatt ggaaaaaaca tagctcctgg attaaacaga aactttgcct gtatggactg
     6901 gcctcaaatt gaaaaagaaa tatggaaaaa agaaaaaacc gctgcctata aagccataga
     6961 tcaggaagtt cagctaaaaa taaccgctta tgatatcgat cctaaagcca tctgtattgc
     7021 aaaagaaaac gcccgggaag caggcgttga tgactgtatc cggtttgaag taaaagatgc
     7081 aaaaaaaata aacattcaag agccttatgg aattttaatc actaacccgc cttatggaga
     7141 acggcttgga gaaagagaag aaatcaaaaa tctatataaa agccttggaa aaatcttcaa
     7201 agacgaatcc acctggtctt tatatttctt aacttctgta gaagaatttg aaaagtattt
     7261 tggcaaaaaa gccaataaaa aaagaaagct ttataatggc cgaataaaag
//
LOCUS       DAOSUD010000094         7305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5050_length_7305_cov_22.124276, whole genome shotgun sequence.
ACCESSION   DAOSUD010000094 DAOSUD010000000
VERSION     DAOSUD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_7305_cov_22.124276"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..461
                     /gene="flgB"
                     /locus_tag="PLG49_09615"
     CDS             90..461
                     /gene="flgB"
                     /locus_tag="PLG49_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOQ17601.1"
                     /translation="MLFSRLNSNVDILEKALDASWLRNEIIAGNIANADTPNYKKKEV
                     KFEQYLADALKSNNKKNVEKVQPRIVVNYDNLETRMDGNNVDVDVEMVEMAKNSIRYN
                     TLISQVSHEFKRINIVLNNIR"
     gene            473..913
                     /gene="flgC"
                     /locus_tag="PLG49_09620"
     CDS             473..913
                     /gene="flgC"
                     /locus_tag="PLG49_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOQ17602.1"
                     /translation="MSFFRAMDVSASGLTAQRLRMDIISQNIANINTTRTENGEPYRR
                     KTVLFEEKKEQLPFSKLLKKSMEQYSSSGVRVSAIVEDNSDFQMVYDPEHPDADENGY
                     VRMPNVNIIEEMVNMISANRSYEANVTAINTNKSMALKALEIGR"
     gene            938..1252
                     /gene="fliE"
                     /locus_tag="PLG49_09625"
     CDS             938..1252
                     /gene="fliE"
                     /locus_tag="PLG49_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOQ17603.1"
                     /translation="MKVEGRNFINSLNLDNNFDNHTTKVNREALVFEDFYKAALGLIN
                     TTNSLQKDAEQITLDFVTGKTDNIHNVMIAQEKANVALQFTVQITNKVLEAYNDIMRI
                     NL"
     gene            1305..2945
                     /gene="fliF"
                     /locus_tag="PLG49_09630"
     CDS             1305..2945
                     /gene="fliF"
                     /locus_tag="PLG49_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOQ17604.1"
                     /translation="MQETFSKISEQITEYWNKFNKGQKIRLGLIAVCIVFSLAVAIFI
                     TSRPKMVRLFNEDLTYEQAAEIKEVLDAANIYNKTNENGTSIEVKKEDYAKAKMELAM
                     EQIPDSGFTFEDALDNGMGTTETEKNAKLKEAEQERLAKEIATIEGVEAANVHLVIPN
                     DDNFFIAANQEASASVKLTLKKELSKKQVLGIAHYVAGSVKNLDVKNVRIIDNRGNIL
                     YLADAETAGVNMDEQQEVKLAAEREIERKVLSILGPLFDDVRVTPNLVLDFDKYREKQ
                     ELVSSPIEGSNKGVISYENTSSRKATGGASGEEPGLAANDLDVPQYVMGAENAYEAKE
                     NQSEIKYELDKKYTELEKSIGSVIADESNISVVVLRDKIYDQEQLESNRGSNNANSMQ
                     DGESWEEFKYRIKSTPSIIDIENEYPQLRSLVANASGLNVNNVEILGYEVPVFIDKTI
                     TPRPYHEYVLLGILFVLIALLAYALIRKAEPIEVTEIEPELSVEAMLESSQVRSEELE
                     PIEYNEESEIKKQIDKFVDEKPEAVASLLRNWLSEEWE"
     gene            2952..3965
                     /gene="fliG"
                     /locus_tag="PLG49_09635"
     CDS             2952..3965
                     /gene="fliG"
                     /locus_tag="PLG49_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOQ17605.1"
                     /translation="MAIVKEELSGREKAAMLLIALGPEKSARIFKHLREEEIEQLTLE
                     IANIRTVSPQMKEKVLDEFYQVCLAQQYIAEGGIAYAKQVLEKALGSEKAIEVINKLT
                     VSLQVRPFEFVRKTDASQLLNFIQNEHPQAIALILSYLKPTQAAAVLASLPQEKQPDV
                     ARRIALMDRTSPDIIKEVEKVLEKKLSALVTEDFTAVGGVDAIVDIINCVDRGTEKYI
                     METLEVEDTELAEEIKKKMFVFEDIITLDSRSIQRILREVDNNDLAIALKGATDEVKN
                     VIFANLSKRLSAMIQEDMEYMGPVRLRDVEDAQQKIVNVIRKLEEVGEIVISRGGGDE
                     IIV"
     gene            3958..4695
                     /locus_tag="PLG49_09640"
     CDS             3958..4695
                     /locus_tag="PLG49_09640"
                     /inference="COORDINATES: protein motif:HMM:NF018358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOQ17606.1"
                     /translation="MSSIIKGPAVNYNYEQTVQIKANEEFPKEDLVKIDNTSAEIIVQ
                     NILEKARYDANKMIHEAEKAAAELLDKARQEAIALKKETEEEAWKEGYEKGLQQGILE
                     GEKIKSQAKEELKKAYLEKERIINEIEPQMVQLIIDILEKLLANSIKTNPQYILYLIR
                     KGFSQVKEGNEKIIIRVSDQDYPLVIESKEELIEDLGINNDIEIIKDSSLSCSDCIIE
                     TDFGNIDCSLGVQFEGLKEELLSIKNY"
     gene            4716..6032
                     /gene="fliI"
                     /locus_tag="PLG49_09645"
     CDS             4716..6032
                     /gene="fliI"
                     /locus_tag="PLG49_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOQ17607.1"
                     /translation="MNTINIDKYRSVLNKDYFKYQGRVSQVVGLTIESIGPVCNVGDL
                     CYIRSKNTSYPVMAEVVGFKNNKILLMPLGEMAGIGPGSIIETTKTNLNVSVGYHLLG
                     RIINGLGQAMDGKELPASDIRYPIENQPPNPLKRKRITEVLPLGVKAIDGLLTVGKGQ
                     RLGIFAGSGVGKSTLLGMIARNTKADLNVIALIGERGREVREFIEKDLKEEGLKRSVL
                     VVATSDQPALIRLKAAQTATAIAEYFRDEGKDVLLLMDSLTRFAMAQREIGLAIGEPP
                     VSRGYTPSVFAVMPKLLERAGNSDRGSITGLYTVLVDGDDMTEPVTDTARGILDGHIV
                     LSRKIANRNHYPAIDILQSVSRVMNDIVDNDHKAMAYEIKKLMAVYRDAEDLINIGAY
                     VKGSNDEIDEAISKIKKINDFLTQKVEENFEMNEVLEQMNRILYGE"
     gene            6051..6518
                     /gene="fliJ"
                     /locus_tag="PLG49_09650"
     CDS             6051..6518
                     /gene="fliJ"
                     /locus_tag="PLG49_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOQ17608.1"
                     /translation="MGRFYFRMESILNVREKIEEQKKQEYSNALNELHREISIKEKLT
                     EESNELIHLLRSKIHDSIAPNEIIFYNNYGNFLKRKIIKQEENVKLAYEKAEKKRQEL
                     IEAFRQKKMLEKLKEKHWFNFLDDENKKEQRSIDEVVSFQSQNRLIDRSETNS"
     gene            6502..7257
                     /locus_tag="PLG49_09655"
     CDS             6502..7257
                     /locus_tag="PLG49_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17609.1"
                     /translation="MRQIASKVSTPTTKNKEEDFLPSEERKKKSPLFIAIIIFIILFL
                     VIAAVIRFNVGNLSEKYLRNFLENVPIISNLLPASKKDGNKYGNLTKEELIKEINNLE
                     SQLERKEEQLASYQNAIERLEAEVKRLREIEEQQLQFKLDKDEFSKQVAQDNPEAFMK
                     FFESIYPDTAAQIYEELLGEEVNTQEIKKYAEPFQTMDPASAAEVVEEMMVTDMDLVV
                     LIFNNIDSEQQAAIIEEMNPERAARLVKSLAPQ"
BASE COUNT         2740 a          970 c         1499 g         2096 t
ORIGIN      
        1 tttaattaat ggaagattga aaaaaattat attaatgtgt ggttttttgt tatataatta
       61 tgattaatta aagcatagga gggttaataa tgttgtttag ccggctaaat tctaatgttg
      121 acattttaga aaaagccttg gatgcttcgt ggttgagaaa tgaaattatc gctgggaaca
      181 ttgctaatgc agatacacca aattataaga aaaaagaagt aaaatttgaa cagtatttag
      241 cagatgctct taagtcaaat aataagaaaa atgttgaaaa agttcagccc cgaattgtag
      301 tcaattacga taatttggaa acacgtatgg atggaaacaa tgtagatgtt gatgtagaaa
      361 tggtggagat ggccaaaaac agcatcagat acaacacttt aataagtcag gtatctcatg
      421 agtttaaaag aattaacatt gtgttaaaca atattagata ggggtgaggt cgatgtcatt
      481 tttcagggca atggatgtca gtgccagtgg tttaacagct caaagattaa gaatggatat
      541 catttctcag aacattgcta atatcaatac gacccgcaca gaaaatggag aaccatatag
      601 aagaaaaacg gttttatttg aagaaaaaaa agagcaactg cctttcagta agcttttaaa
      661 aaagagcatg gaacaatata gtagttctgg ggtacgggtt tcagcaatcg tagaggataa
      721 tagtgatttt caaatggtat acgaccctga acatcctgat gcagatgaaa acggatatgt
      781 tcgcatgcct aatgttaata ttattgaaga aatggtaaat atgatttctg ctaaccgctc
      841 ctatgaagct aatgttacag ctatcaatac caataaaagt atggcgttga aagcattgga
      901 aattggtcgt taatttatct gatttaggag gatgtacatg aaggttgaag gtcggaattt
      961 tattaattcc ttaaatttag ataataattt cgataatcat actactaaag ttaatagaga
     1021 agcccttgtt tttgaagatt tttataaagc agcgttaggc ttaattaata ctaccaattc
     1081 tcttcaaaaa gatgcagagc aaattacttt agattttgta accggtaaaa cggataatat
     1141 acacaatgtc atgattgcac aggaaaaagc aaatgtagca cttcaattta ctgtacaaat
     1201 caccaataaa gtattagaag cttataatga catcatgcgg atcaatttat aatgttaagt
     1261 tataagcgta taatactgat tttgtacaga aatgaggtga atgaatgcag gaaacttttt
     1321 cgaagatttc agagcagatt acagaatatt ggaataaatt caataaagga caaaaaatta
     1381 ggttagggct catagctgta tgtattgttt tttctttggc tgttgctatt tttattacca
     1441 gcagaccgaa aatggtaaga ttatttaatg aagatcttac ttatgagcaa gctgcagaaa
     1501 ttaaagaagt attagatgcg gccaatattt ataataaaac caatgaaaat ggaacatcca
     1561 ttgaagtaaa aaaagaagac tatgctaaag ccaaaatgga attggctatg gagcaaatcc
     1621 cagatagtgg ttttactttt gaagatgcac ttgataatgg tatgggaaca acggaaacgg
     1681 aaaaaaatgc caagttaaaa gaagctgaac aagagaggtt agcaaaggaa attgctacta
     1741 ttgaaggtgt tgaggcggcc aatgtgcatt tagttatacc aaatgatgat aattttttca
     1801 tagcggctaa tcaggaagca agtgcaagtg ttaagttgac tcttaaaaag gaattaagca
     1861 aaaagcaagt tttaggaatt gctcattatg ttgcaggaag cgttaaaaat ttagatgtaa
     1921 aaaatgtaag gattattgat aacagaggta acatcttata tcttgctgat gctgaaacag
     1981 caggtgttaa tatggatgaa cagcaggaag ttaaacttgc tgcagaaaga gaaattgaaa
     2041 gaaaagtact ctcaattctg ggtcctttat ttgatgatgt gcgggtaact cccaatttgg
     2101 tattagattt tgataagtat agggaaaaac aagaattagt cagctctcct atagaaggat
     2161 caaataaagg cgttatttct tatgaaaaca cttcttcgcg caaagcaact ggtggtgctt
     2221 caggagaaga accaggctta gcggctaatg atcttgatgt tccacaatat gttatgggtg
     2281 ccgaaaacgc ttatgaagct aaagaaaacc aaagtgaaat aaagtatgaa ctagacaaaa
     2341 aatatactga attagaaaaa agtatcggtt ctgtaatagc agatgaatcg aatatttcgg
     2401 ttgttgtatt acgggataaa atttacgatc aggagcaatt agaaagcaat cgtggttcaa
     2461 ataatgcaaa ttcaatgcag gatggagaaa gttgggaaga atttaagtat cgtattaaat
     2521 cgaccccttc catcattgat attgaaaatg aatatccgca attacgtagt ttggtggcaa
     2581 atgcatcggg tttaaatgta aacaatgttg agattcttgg atatgaggtt cctgttttta
     2641 tagataagac cattacacca aggccttatc atgaatatgt tttattgggt attttatttg
     2701 ttctgattgc attgttagct tatgctttaa ttagaaaagc agagcctata gaagttacag
     2761 aaatagaacc tgaattgtct gtagaagcaa tgcttgaaag ctcacaagtt cgttcagaag
     2821 aacttgaacc cattgaatat aatgaagaat ctgagatcaa gaaacaaatt gataaatttg
     2881 ttgacgaaaa acctgaagca gttgcaagtc ttttacgcaa ttggctaagc gaagaatggg
     2941 agtgaagata gatggcaatt gtgaaggagg aactttcagg ccgagaaaaa gcagctatgc
     3001 ttcttattgc attgggacct gaaaaatcag ctcgtatatt taaacattta agggaagaag
     3061 aaatagagca attaacttta gaaatagcaa atatacggac tgtttcccca caaatgaagg
     3121 aaaaagtttt agatgagttt tatcaggttt gtttagcgca gcaatatatt gcagaaggag
     3181 gtattgctta cgctaaacag gttcttgaaa aagcgttggg cagcgaaaaa gccattgagg
     3241 ttattaataa acttaccgta tctcttcaag tcagaccttt tgaatttgta agaaaaacgg
     3301 atgcaagtca gctattaaac tttattcaaa atgaacatcc tcaagcaatc gcccttattc
     3361 tatcttattt aaaacccact caggcagctg cagtattagc atctttacct caggaaaaac
     3421 aacctgatgt agcaagaaga attgctttaa tggataggac atcgccagac atcatcaaag
     3481 aggtcgaaaa agttttggaa aagaaactct ctgctttagt aacagaagat tttactgctg
     3541 tcggcggtgt agatgctatt gttgatatca taaattgtgt ggacagggga actgaaaaat
     3601 atattatgga aacactggaa gtagaagata ctgaactagc agaagaaatt aagaagaaga
     3661 tgtttgtatt tgaagatatt attaccttag acagcaggtc aattcaaaga atactcagag
     3721 aagttgataa caatgattta gcgattgccc ttaaaggagc aaccgatgaa gttaaaaatg
     3781 ttatctttgc aaatctttct aaacgtctct ctgccatgat acaagaagat atggaatata
     3841 tgggacctgt tcgacttagg gatgtcgaag atgctcaaca aaaaattgtt aatgtaatca
     3901 ggaaacttga agaagttggc gaaattgtta tttccagagg tggaggtgac gaaataattg
     3961 tctagtatca taaaaggtcc tgctgttaac tataattatg agcaaacggt acagattaaa
     4021 gccaacgagg aatttcctaa agaagatctt gttaaaattg ataatacttc ggctgaaatc
     4081 atagtccaga atattttaga aaaagcaaga tacgatgcta ataaaatgat ccatgaggca
     4141 gaaaaagcag cagctgagtt gttggataaa gcccgtcagg aagcaatcgc attaaaaaag
     4201 gaaacagaag aagaagcctg gaaagaaggt tatgaaaaag gattgcaaca aggcatcctg
     4261 gagggcgaaa aaattaaaag tcaggcaaaa gaagaattaa agaaagctta tttagaaaaa
     4321 gaaagaatca ttaatgaaat agaaccccaa atggttcagc tcattattga tattttggaa
     4381 aagttattag cgaatagcat aaaaacaaac ccgcaatata tcttatattt aataagaaag
     4441 ggtttttctc aggttaaaga aggaaatgaa aaaataatta tccgggtttc tgatcaagac
     4501 tatccgttgg ttattgaatc caaagaggaa ttgatagaag atttaggaat aaataatgat
     4561 attgaaatta ttaaagattc ttcattatct tgttcagatt gtattatcga aacagacttt
     4621 ggtaatattg actgcagttt aggcgtgcaa tttgaaggat taaaagaaga attactgtca
     4681 attaagaatt attgatttta ggattgggtg aggtcttgaa tacaatcaac atcgacaaat
     4741 acaggagcgt acttaataaa gattatttta aatatcaggg aagagtatca caagttgttg
     4801 gtttaaccat cgaatccata ggaccagttt gtaatgtggg agatctttgc tatattcgtt
     4861 caaaaaatac ttcttatccc gtaatggctg aagtggtagg ttttaaaaat aataaaattc
     4921 tcttaatgcc tttgggagag atggctggca ttggtcctgg cagtattata gaaaccacta
     4981 aaacaaattt aaatgtttct gttggctatc atttattagg aagaattata aacggactgg
     5041 gacaagctat ggatggtaaa gaattacctg cttcagatat aagatatccc attgaaaatc
     5101 aaccgcctaa tcctttaaag cgcaaacgga taacagaagt tcttccttta ggtgttaagg
     5161 caattgatgg acttttaacc gtaggaaaag gtcaaagatt aggaattttt gccggtagtg
     5221 gtgtgggaaa aagtacctta ttaggtatga ttgccagaaa cacaaaagct gatttaaatg
     5281 tgattgcttt aatcggagaa aggggaagag aagtaagaga gtttatagaa aaagatttaa
     5341 aagaagaagg actaaagcgt tcagttcttg tcgttgcaac ttctgaccag cctgcattaa
     5401 tccgtttaaa agctgctcaa acagcaactg caattgctga atatttccgg gacgaaggga
     5461 aagatgtatt gctgcttatg gattctctga cacgttttgc aatggctcaa agagaaatcg
     5521 gtttagccat tggtgaacca cctgtatcaa gaggctacac cccttctgtt tttgcagtta
     5581 tgcctaagct tttggaaaga gcaggtaatt ctgatcgggg ttcgattacc ggtttatata
     5641 cggttttagt tgatggcgat gatatgacag aacctgtgac agatacagca cgtggaattc
     5701 tggatggtca tattgtctta tccagaaaaa tagctaatag gaatcattat ccagctatcg
     5761 atattttaca aagtgtatcc agggtaatga acgatattgt tgataatgac cataaagcaa
     5821 tggcatatga aataaaaaaa ttaatggctg tttatagaga tgctgaagat ttaattaata
     5881 ttggtgcata tgttaaagga agcaatgatg aaatcgatga agctatttct aaaataaaaa
     5941 aaattaatga ttttttaacg caaaaagttg aagaaaattt cgagatgaat gaagttttag
     6001 agcaaatgaa tcgaattctt tacggagaat agatattaaa ggggattttt atgggccgtt
     6061 tttatttccg tatggaaagc attttgaacg taagagaaaa aattgaagaa caaaaaaagc
     6121 aggaatattc aaatgcctta aatgaacttc atagagaaat atcaataaaa gaaaaattga
     6181 cagaggaatc taacgaattg attcatttat tacgttcaaa aatccatgat tcaattgctc
     6241 ctaatgaaat aattttttat aataattatg gtaacttttt aaaaagaaaa ataatcaagc
     6301 aagaagaaaa tgtaaaactt gcttatgaga aggcagaaaa aaaaaggcaa gaactaattg
     6361 aagcattcag gcaaaagaag atgctggaga aattaaaaga aaaacactgg tttaatttct
     6421 tagatgatga aaataaaaaa gaacaacgaa gtattgatga agtcgttagt ttccaatctc
     6481 aaaaccgtct tatagatagg agtgagacaa atagctagta aagtaagcac cccgaccaca
     6541 aaaaataaag aggaagattt tcttccatct gaagagagaa aaaagaaaag tcctttattt
     6601 attgcaatta ttatatttat tatcctattt ctcgtaatag cggctgtaat ccgttttaat
     6661 gtcggaaact tatcggaaaa atatttaaga aattttttag aaaatgtacc tattatcagc
     6721 aatcttcttc ctgccagcaa aaaagatggt aacaagtatg ggaatttgac taaagaagaa
     6781 ttaatcaagg aaataaataa cttggaaagt caattagaaa gaaaagaaga acaacttgct
     6841 tcttatcaaa atgccataga aagacttgaa gctgaagtga aaagactgag ggaaatagaa
     6901 gagcagcaat tacaatttaa gctagacaaa gatgaattta gtaagcaggt tgcacaggac
     6961 aatcctgaag cttttatgaa gttttttgaa tctatttatc cggatacagc agcacaaatc
     7021 tacgaggaat tactgggtga agaagttaat acacaagaga ttaaaaaata tgctgaaccg
     7081 ttccaaacca tggaccctgc cagtgctgca gaagttgttg aagaaatgat ggttacagat
     7141 atggatttgg ttgttcttat ctttaataac atagatagtg aacaacaggc tgcaattata
     7201 gaagaaatga atcctgaacg tgcagccaga cttgtaaaaa gtttagcacc acaataatga
     7261 aatgaaagga gggtagggga tgaatgtaca aattttttca tttga
//
LOCUS       DAOSUD010000095         7182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5135_length_7182_cov_21.121510, whole genome shotgun sequence.
ACCESSION   DAOSUD010000095 DAOSUD010000000
VERSION     DAOSUD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5135_length_7182_cov_21.121510"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLG49_09660"
     CDS             <1..290
                     /locus_tag="PLG49_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOQ17610.1"
                     /translation="PSIHLIRADIETEVSPIVGTEKQSEELVNYLMSEFETDPRKIWE
                     SNIFGKSLHELVNEGLQNKLYRMPEDAQMKLQETLQKIINEGHGGLICIIL"
     gene            475..666
                     /locus_tag="PLG49_09665"
     CDS             475..666
                     /locus_tag="PLG49_09665"
                     /inference="COORDINATES: protein motif:HMM:NF040112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HOQ17611.1"
                     /translation="MREEVYFQFNGKEVLHSDLIKQFKEKWKQDNLKIKDVKSLKLYY
                     NADEEKCYYVVNDKESGTL"
     gene            764..2260
                     /locus_tag="PLG49_09670"
     CDS             764..2260
                     /locus_tag="PLG49_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOQ17612.1"
                     /translation="MAKKKYKKIKRQHKRRIVLGLFLSSCVIVYLIGALLRFKSITPL
                     SIEVAQIGEIDRNIHLSGIIVRDEKVVKSKEEGNITYLAFEGEKVKPGTKICMVGDST
                     VIAKIQEDIKKINEDILKEQNQRKEFSLVRKDIKNINYQIDDLVHKYMSTYSNRNYSQ
                     LYAIKSSIELEIEKKQSVLAQENSSALQELVALKKEYENKLFNNSDVITAPKGGIVSY
                     YYDGLEEKFSLDNFAQFSEKDLKTSAELVDLSQNHNVKKDSPVFKIVDNYSWYILGTF
                     SKELVEDYQVGDMIAIKLNGIHDSNLKAEIYNLKPLNDETMLFILRCTEQMPPLMFGR
                     NIEFEILRDNYEGIKIPVNAIVEKVFLKVPKKYLQDSGSNKVIIKRLPDKDELIPVKI
                     EYEDDNHVYILEDFSSVKLYDTIVLPENAQEEYVINQSESKEGVFVVNGGIVRFKTIK
                     ILAQNDEYAIVDGSLNNGIKIYDQIVSNAKNVKENQLINQFNIINNQQ"
     gene            2289..2987
                     /locus_tag="PLG49_09675"
     CDS             2289..2987
                     /locus_tag="PLG49_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOQ17613.1"
                     /translation="MSDIKKNLEVIKEKIDRAAQKSGRKGTDITVIAVTKTIDVARIN
                     ELISYGIQNIGENKAQELRDKYPQIDPSVNWHFIGHLQSNKIKYIIDKVKLIHSVDSE
                     KLAYNINEAAKKHDKVMDILLQVNVAEDENKFGLSYKEVEPLLYKLKDLKNIRVRGLM
                     TIVPYDEEAENNRLYFRRMKQLAVDIDNQNIDNISMEFLSMGMTNDFEIAIEEGSNMV
                     RIGTGIFGARNYNM"
     gene            3000..3464
                     /gene="sepF"
                     /locus_tag="PLG49_09680"
     CDS             3000..3464
                     /gene="sepF"
                     /locus_tag="PLG49_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOQ17614.1"
                     /translation="MAKFIDKMMDVMGFGSDEDFEEEVEEIEEKTEVPNIRSLRSRSG
                     GNSKIVNIHTNIKMEVVITDPEAYEEAQEICDHIKSKKPVVINLENMDRQTAQRVMDF
                     LSGACYALNGDVQRVANNIFIIAPENVDIANSFREELKTKGIILPWANGGMK"
     gene            3477..3770
                     /locus_tag="PLG49_09685"
     CDS             3477..3770
                     /locus_tag="PLG49_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOQ17615.1"
                     /translation="MLGIVLYYLAVYFFRLLEILIFIRVVLSWLPVKRQHTFVQLIYK
                     LTEPLLAPSRNLLEKSAIGASRLMVDFSPVITLIVLDFIRMCILYFIGKYLIF"
     gene            3808..4596
                     /locus_tag="PLG49_09690"
     CDS             3808..4596
                     /locus_tag="PLG49_09690"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOQ17616.1"
                     /translation="MINKDLYLGKFYDLEEKITASNILDKVNICLRDHVNTFTNFIDM
                     YKIGKYLEMLSSFRDINLRAFGGYPDSERNIIGLSPCYKELEESDFPIAAIEINLRNP
                     KAYPVSHRDYLGSVLSLGIDRKKIGDILVQENKAIVFAFEEVADYILNHLTRIRNIGA
                     SAQRISLNDIKVPTPEIKEVISTVSSLRADAVLSSGFNLSRSKVVELIKAEKALINGS
                     IAEPSSTVQCGDYLTLRGYGKIKLSEVKGKTKKGRISVVIHCYK"
     gene            4609..5301
                     /locus_tag="PLG49_09695"
     CDS             4609..5301
                     /locus_tag="PLG49_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOQ17617.1"
                     /translation="MLTPLDIETREFKRKPYGYSIEEVDKFLIEVINCYERLYKENIE
                     LKDKISILNDGISHYKSLEETLQSTLVLAEKTAEETKSAAYQKGEQIINAAEIKANQI
                     LEQAKQEVFKIKQEIERLKKQYVAAKIQMMQILNSQLQLLEQSSLFEEEESDNNQLVK
                     RKDDKLNSDEGKETNQYKDIDISKGLKKFDEDNEDMESDQGEAGLMFDDYEDYKELNQ
                     QFRLARRDEDAI"
     gene            5298..6389
                     /gene="aroB"
                     /locus_tag="PLG49_09700"
     CDS             5298..6389
                     /gene="aroB"
                     /locus_tag="PLG49_09700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOQ17618.1"
                     /translation="MNEICINTPSSAYPIYFSDNFEDIINALKKFFPSDRKICIVTDE
                     NVNKYYGQTLLEIVSSAYKEVYKCVFPAGESNKNLNTVQEIYKFFIEKHLDRNSLILA
                     LGGGVTGDMAGFAAATYMRGIPFVQIPTTLLSQVDSSVGGKVGVDFLQHKNMIGAFYQ
                     PAFVYINLDTLKTLPKEQLSSGVAEVIKHGLILDKSYFEFLESSYDSFFKLDHEVLEK
                     IIRRSCELKGSVVSRDEKEAGLRAILNFGHTLGHAIETLLNFKLFHGQCVSVGMVGAA
                     YLSYINGLISKEDLKRIEKVLASYDLPVRIEELNKEVIYNQMLLDKKTKNNIIHFILL
                     SSIGKAVRVSHVNTDQIFSAIAYIQNDVL"
     gene            6531..7166
                     /locus_tag="PLG49_09705"
     CDS             6531..7166
                     /locus_tag="PLG49_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOQ17619.1"
                     /translation="MDDKMIKELEAILLKEKAEAEVQLRKMKENGSKTSLLEWTGELS
                     SYDNHPADIGSESFEMEKMMTLNNNQQKLLDDINDAIEKIEKGTYGICETCSSEIDYE
                     RLKAIPYARECINCAKARLTNEDEYQRRRPIEEELLEPIIDNGLVQGDELDPALEVYR
                     HLQVYGSADNMQDMGGPIKDYDDIHNIYDASIESIEEMDVLTNKDRKNSLY"
BASE COUNT         2649 a          886 c         1414 g         2233 t
ORIGIN      
        1 ctccttcgat tcatttaatc cgggcagata tcgaaacaga agtatcgcca atcgtaggaa
       61 ctgaaaaaca aagtgaagaa ctggtaaatt atttaatgag tgaatttgaa actgatccaa
      121 ggaagatatg ggaatctaat atttttggaa aatctcttca cgaactggtc aatgaagggc
      181 tccagaacaa actgtaccgt atgcctgaag atgctcaaat gaaattacaa gaaactttgc
      241 aaaaaataat taatgaggga catggcggtc taatttgcat tattttataa agatttgata
      301 ggcaaataat catgattttg atggggctaa agtgctaagg aactgccctt gacaataaga
      361 gagatatggt ataaaattat tattaaaaat acacaataat actaatatat ataatattac
      421 aaatttttct tttatctata aataataccc tagggggcag aatggaggct gaagatgagg
      481 gaagaagtat attttcagtt taatggcaaa gaagttcttc atagcgattt gattaaacaa
      541 tttaaagaaa aatggaagca ggataattta aaaattaaag acgtaaaatc cctgaagtta
      601 tattataatg ccgatgagga aaagtgctat tatgttgtta atgacaaaga aagcggaact
      661 ttgtaaaaca ctcttatcag agtgtttttt tacttgtttt ttgggactca atccgttata
      721 ttagtaaata aatagtataa taactattag gtgtgaggat tatatggcaa aaaaaaaata
      781 caaaaaaatt aaaagacaac ataaaagaag aattgtctta ggtttatttt tatcttcatg
      841 tgtcattgta tatttaattg gtgccttatt aagatttaaa agtattaccc ccttatctat
      901 cgaagttgct caaataggag aaattgacag gaatatacat ctaagtggta tcatcgtcag
      961 ggatgaaaaa gttgtaaaaa gtaaagaaga aggcaatatt acttatttag cttttgaagg
     1021 tgaaaaggta aaaccaggta caaaaatttg tatggtagga gatagtactg tcatcgcaaa
     1081 aattcaggaa gatattaaaa aaatcaatga agatattctt aaagagcaaa accaaagaaa
     1141 agaattttct ttagtaagaa aagatattaa aaatattaat tatcaaattg atgatttagt
     1201 tcataaatat atgagtacat attcaaacag gaattattct caattatatg ctattaaaag
     1261 ttctattgaa ttagaaatag aaaagaaaca gtctgtttta gcgcaagaaa attcctctgc
     1321 ccttcaggaa ttagtggcct taaaaaaaga atatgaaaat aaattattta acaattctga
     1381 tgttattaca gcacctaaag gtggtattgt ttcttattat tatgatggcc tggaagaaaa
     1441 gttttcttta gataattttg ctcagttcag tgaaaaagat ttaaagactt ctgctgagtt
     1501 agttgattta agccaaaatc ataatgtgaa aaaggattct cctgttttta aaattgtcga
     1561 taattattca tggtatatct tagggacttt ttccaaagaa ttggtagaag attaccaggt
     1621 tggggatatg atagccataa aacttaatgg aattcatgat tctaacttaa aagcagaaat
     1681 ttataattta aagcccctta atgatgagac gatgctcttt attttaagat gtactgaaca
     1741 aatgcctcca cttatgtttg gcagaaatat tgagttcgaa attctcaggg ataattatga
     1801 aggaattaag attcctgtta atgctatagt tgaaaaagta tttttgaagg tacctaaaaa
     1861 atatcttcag gactcgggaa gcaataaggt aatcattaaa agacttcctg ataaagatga
     1921 attgattccg gtaaaaattg aatatgaaga cgataatcat gtttatattc ttgaagattt
     1981 ttcatctgta aagctttatg atactatcgt tttaccagaa aatgcgcaag aagaatatgt
     2041 cattaatcaa agtgagtcaa aggaaggcgt atttgttgta aatggtggta tcgtacgttt
     2101 taaaaccatc aaaattttag cgcagaatga tgaatatgct attgtcgatg gtagtttaaa
     2161 caatggtatt aaaatatatg accaaattgt atctaatgca aaaaatgtta aagaaaatca
     2221 gttgatcaat caatttaata tcattaataa tcaacagtag accaatgaaa aggcaggatg
     2281 gtgattgaat gagtgatatt aaaaagaatc tggaagtgat taaagaaaaa attgacaggg
     2341 ctgcacaaaa aagtggccgt aaaggaacag atataactgt tattgcagta accaaaacca
     2401 ttgatgtagc aagaatcaat gaattaatat cttatgggat tcaaaatatt ggggaaaata
     2461 aagcccagga gctgagagat aaatatccgc agatagatcc ttcagtcaac tggcatttta
     2521 tcggtcatct tcaaagcaat aagattaaat atattattga taaagtgaaa ttaatccatt
     2581 cagtagatag tgaaaagctg gcttataata ttaatgaagc agctaaaaag catgataaag
     2641 tcatggatat tctgcttcaa gtcaatgtgg ctgaagatga aaataagttt ggtttatctt
     2701 ataaggaagt agaaccatta ttatacaaat taaaagattt aaagaatatt cgtgtaaggg
     2761 gtcttatgac catcgttcca tatgatgaag aagctgaaaa taacagactt tattttagaa
     2821 ggatgaagca attagcggtt gacattgata atcaaaacat cgataatata agtatggaat
     2881 ttctttctat gggaatgaca aatgactttg agatagctat tgaagaaggc tcaaatatgg
     2941 tacgtatagg aacgggtatt ttcggtgcca gaaattataa tatgtaagga ggtattcaca
     3001 tggcaaagtt cattgacaaa atgatggatg taatgggatt tggcagcgat gaagactttg
     3061 aagaagaagt agaagaaatt gaagaaaaga ctgaagtacc taacattagg agtttacgtt
     3121 ctcgtagtgg cggtaattca aaaattgtaa atatccatac aaatattaaa atggaagttg
     3181 tcataacgga tcctgaggca tatgaagaag ctcaggaaat atgtgaccat attaaaagta
     3241 aaaagcctgt agtgattaac cttgaaaata tggaccgcca gacagcacaa cgtgtgatgg
     3301 attttttaag cggtgcttgt tatgcactga acggtgatgt gcaaagagta gctaataata
     3361 tctttattat tgctcctgaa aatgtagata ttgctaatag ttttagagaa gaattaaaaa
     3421 cgaaaggaat tattcttccc tgggcgaatg gtggaatgaa ataacggagg ataataatgt
     3481 tgggcattgt tttatactat cttgcagtat attttttcag actattagaa attcttattt
     3541 ttatacgggt tgttttatcc tggcttcctg ttaaacggca gcatactttt gttcaattaa
     3601 tatataaatt aacggaacct ttactggcgc cgtcacggaa tttgctggag aagtctgcga
     3661 ttggtgcaag caggctcatg gttgattttt caccagtgat aacactcatt gttttagatt
     3721 ttatcaggat gtgtatttta tattttattg gtaagtattt aattttttaa ggacaaaatt
     3781 ttcatcatat gttggtgatt acttctaatg attaataaag atttatacct cggaaagttt
     3841 tatgacttgg aggaaaaaat aacagcaagc aatattttag ataaagtaaa tatttgttta
     3901 agagatcatg tcaatacttt tacaaatttt attgatatgt ataagatagg caagtatttg
     3961 gagatgcttt cttcctttag ggatataaac cttcgggctt ttggcggata tccggacagt
     4021 gagcggaata ttattggttt atctccctgt tataaagaat tggaggaaag cgattttcct
     4081 attgcagcca ttgagattaa tttaagaaat ccaaaagcat atcctgtttc tcacagggat
     4141 tacctggggt cggtgttatc tttagggata gaccgaaaaa aaataggaga tattttggta
     4201 caggagaata aagccattgt atttgctttt gaagaagttg ctgattatat attaaatcat
     4261 ttaaccagga ttagaaatat aggggccagt gcacaaagaa tttctttaaa tgatataaag
     4321 gtgcccacgc ctgaaataaa agaagtcata tctacagtat cttctttaag ggcagatgcc
     4381 gttttaagtt ctggctttaa tttatcgagg tcaaaagtgg ttgaattaat taaagctgag
     4441 aaagcattaa ttaatggcag tattgcagaa ccttcttcaa ctgttcaatg tggggattat
     4501 ttaactttaa gaggatatgg aaaaattaaa ttatctgaag taaagggtaa aactaaaaag
     4561 ggtcgtatta gtgtcgtaat tcactgttat aaataggagg aacataatat gctcacacca
     4621 ttagacattg aaacccgaga gtttaaaaga aagccatatg gttattctat tgaagaagtg
     4681 gacaagtttt taattgaagt catcaattgt tatgaaaggc tttataagga gaatattgaa
     4741 ttaaaagaca aaatttctat tttaaacgac ggtatcagtc attacaaatc tttggaggaa
     4801 acgcttcaaa gtactttggt gcttgctgaa aagacagcag aagaaacaaa atcggctgct
     4861 tatcaaaaag gtgagcaaat tattaatgcg gccgaaatta aggccaatca gattttagaa
     4921 caggcaaaac aagaagtctt taaaatcaaa caagagatag agcgtttgaa aaaacagtat
     4981 gttgctgcaa aaattcagat gatgcagatt ttaaacagtc agttgcaatt attagagcaa
     5041 tcttctttgt ttgaggaaga agagagtgat aataatcaat tggtaaagcg taaagatgat
     5101 aaactcaatt cagatgaagg taaagaaacg aatcagtata aagatataga tatcagtaaa
     5161 ggattgaaaa agtttgatga agacaatgaa gatatggaat ctgaccaggg agaagctgga
     5221 ttaatgtttg atgattacga agattacaaa gaattaaatc aacaatttag attagcaaga
     5281 agagacgagg atgctatatg aacgaaatct gtataaatac gccatcttcg gcatatccta
     5341 tttatttttc cgataatttt gaggatataa taaatgcttt aaagaaattt tttccttccg
     5401 atagaaaaat ctgcattgta accgatgaaa atgttaataa atactatgga cagacactgt
     5461 tggaaattgt ttcttctgct tataaagaag tgtataaatg tgtttttcct gcaggagaaa
     5521 gcaataaaaa tctaaatact gttcaggaga tttataagtt ttttatagaa aaacatctgg
     5581 acagaaattc cctaatcctt gctttaggcg gaggggttac tggtgatatg gctggttttg
     5641 ctgcagcaac ttatatgaga gggattcctt ttgtccaaat accaacaact cttttatctc
     5701 aggtagatag tagtgttggt ggtaaggtcg gggtagattt tcttcagcac aaaaatatga
     5761 ttggagcttt ttatcaacca gcttttgttt atattaattt agatactttg aaaactttgc
     5821 caaaagagca attgtcttct ggtgttgcag aagtcataaa acatggactt atattagaca
     5881 aatcttattt tgaatttttg gagtcttcat atgattcatt ttttaaatta gatcatgaag
     5941 ttttagaaaa aattatcagg cgctcctgtg aattgaaagg aagcgttgta tccagggatg
     6001 aaaaggaagc aggtttaagg gcaatattaa actttggtca tacccttgga cacgctattg
     6061 aaacccttct taattttaag cttttccatg gacagtgcgt ttctgttggt atggttggag
     6121 cagcctattt atcgtacatc aatggtttaa tctcaaaaga agatttaaaa cggattgaaa
     6181 aagttttagc ttcttatgat ttgcctgtca ggatagaaga actgaataaa gaagttattt
     6241 ataatcaaat gttgctggat aagaaaacaa aaaataatat tattcatttt attttgttgt
     6301 cgtctattgg aaaagcggtt agagtaagtc atgtaaatac tgaccagatt ttttccgcta
     6361 ttgcttacat acaaaacgac gtgctttagc acgtttttct tatgtgttgc ctccagtttc
     6421 cattatgctt tttatcagcc tgggaaagct aaagataaga aggctgatgg gaaaaggatg
     6481 aagtataaag gaaaaaagta taaaaaatta aataaaatgg aggcaagtat atggacgata
     6541 aaatgataaa agagttagag gcgattttat taaaagaaaa ggctgaagcg gaagttcaac
     6601 ttagaaaaat gaaagaaaat ggatcaaaaa cttccctttt agaatggact ggggaattat
     6661 ccagttatga taatcatcct gcggacattg gcagcgaaag tttcgaaatg gaaaagatga
     6721 tgacattaaa taataatcag caaaaactct tggatgatat caatgatgct attgaaaaaa
     6781 tagaaaaggg aacctacggt atttgcgaaa cttgttcttc tgaaattgat tatgagcgtt
     6841 taaaggcaat tccttatgca agagaatgta ttaactgcgc taaggcacgc cttacaaatg
     6901 aagatgaata tcaaagaagg cgtccaattg aagaggagct tttagaacca ataattgata
     6961 atggattagt ccaaggagat gaactggatc ctgccctgga agtttacagg catttacagg
     7021 tttatggttc agctgataat atgcaggata tgggaggacc aattaaagat tacgatgaca
     7081 ttcataatat ttatgatgct tcaattgaat ccatcgaaga gatggatgtg ttaacgaata
     7141 aggatagaaa gaactcttta tattaaatac tatgtaaaaa at
//
LOCUS       DAOSUD010000096         6948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5300_length_6948_cov_23.325693, whole genome shotgun sequence.
ACCESSION   DAOSUD010000096 DAOSUD010000000
VERSION     DAOSUD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_6948_cov_23.325693"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..445
                     /locus_tag="PLG49_09710"
     CDS             71..445
                     /locus_tag="PLG49_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOQ17620.1"
                     /translation="MQNLSQLLQTGDWKGEKHVPVIHIPQNVKAGEPIEIKVSIGDEI
                     KHPNELEHHIKWIKLFYFKKDGKFPVEIASFDFVSHGEGGVITEPVASVNVKLNDSGF
                     IYALAYCNIHGLWENKEEIVFE"
     gene            549..1652
                     /locus_tag="PLG49_09715"
     CDS             549..1652
                     /locus_tag="PLG49_09715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ17621.1"
                     /translation="MFTNFIQNLYPIFIEEDFELLSEGEEFDIFSDENEMIRFAKREN
                     TMLYIVNIWNMEKVDLDTFIFRKNAFKKKLEQVFEELHCSYLIVLNLLVTDEEIPFIK
                     PEEFQPNKEIYTISWIIDLQYKKLIIPKKEIDDVLNIRELIHKVFRIIDPSLEKEADD
                     LITRKNERKKRYFLRQRTNNTFLTYVFIILNVIIWLLMELNGGSEDINTLLLFGANEP
                     FLVIERQQYWRLITSTFLHIGISHLLYNTFALYIFGSRIEKYYGKVAFLIIYFISGMI
                     GSMASLIFSDTVSAGASGAIYGLMGALLMIAYKTKKEVDGLNAFTILIMIMLGVAFGF
                     LMPFIDNPAHIGGLIAGMISGRLILQKINKKNY"
     gene            1767..2312
                     /locus_tag="PLG49_09720"
     CDS             1767..2312
                     /locus_tag="PLG49_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOQ17622.1"
                     /translation="MLKLFYVCFYIGIIYTAATFILGYIFDFMGLDGDIGFDGDFFGY
                     GISPIKPVVIAVFLTIFGGVGIIAHKNNLTSRLSLILALVIAISISFLIFRFILVPLH
                     RMQSKEVVEQNELIGHIAKAVLTIKEDECGKIVYTINGNTYSAPARSENKETIDKGEE
                     VVIVNIEHNIFYVKKLKEEGF"
     gene            2313..3758
                     /locus_tag="PLG49_09725"
     CDS             2313..3758
                     /locus_tag="PLG49_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ17623.1"
                     /translation="MDSALMIAAVVVGVIILLILSITMMWKKVPQDKALVITGLKKRV
                     ISGGGGFVIPLLERTDVISLENMKIVVRTEGALTEQGVDIIADGVAVIKVKSDRESIL
                     AAVEQFNTGSEKTTINFIKDTANDVLEGKLREIISKMTVEEIYKDREKFASQVQEVAA
                     VDLAEMGLEIKAFTIRDISDKNGYLEALGKKRIAEVKRDANIAEAEANKETKIKTAEA
                     ARFGEEARIKAETQIAEAAKEKELRIQAFRKEQETEKAKADLAYEIETSKIQQEVEKE
                     KMQIQIVKKEKEIELAEQEAIRKEKELEATIIKQADAEKYREEKRAEALRYQEIQEAM
                     AKAEAIKAMGAAKAEASRLEGMAEVEIIREKGKAEAEAMLKKAEAYKLYNEAAVTEMI
                     IDKIPEIARAIAEPLSKVEKIVIVDSGNGEGKGASKITGYVTDIISSLPETVEALTGV
                     DLKKIVSNRDLFKELENKKDIIEKEDQVNNE"
     gene            3864..5117
                     /locus_tag="PLG49_09730"
     CDS             3864..5117
                     /locus_tag="PLG49_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOQ17624.1"
                     /translation="MNAEILAVGTELLLGNIVNTNAQYIAKRLADIGIDVYFQSVVGD
                     NSGRLYKAFELALQRADIVITTGGLGPTQDDLTKEIAAEISGKKLLLDDKALRMIEEY
                     FSRMGRTMTENNKKQAYIPEASKVFYNYRGTAPGCAIEMGGKIIILLPGPPREMEEMF
                     ERSVIPYLSQFQEGVLASKILRVCGVGESKVEELLADLLDSQTNPTIALYAKFGEVSI
                     RITAKAETEEKAKDLICPVENEIRKILGNHIYGEGDQNLEDVFGELLIKHKKSIAVAE
                     SCTGGLLAGRLINYPGISAVFKEGVVAYSNEAKIRRLGVSEATLSQYGAVSKETALEM
                     AKGICIKAGTSIGISTTGIAGPGGGSIEKPVGLVYAGLCIDGELYSVKFNFTGKRQEI
                     RERTVISVLDWARKILIEKLNSGKF"
     gene            5270..6328
                     /gene="recA"
                     /locus_tag="PLG49_09735"
     CDS             5270..6328
                     /gene="recA"
                     /locus_tag="PLG49_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ17625.1"
                     /translation="MTSERQKALDAAISQIQKQFGKGSIMKLGDTIDNDLSTISTGSL
                     SLDIALGVGGIPRGRIVEIFGPESSGKTTVALHMIAQTQKAGGIAAFIDAEHALDPSY
                     AEKLGVNINELYISQPDYGEQALEIAETMVRSGAIDIVIVDSVAALVPKAEIEGEMGD
                     SHMGLQARLMSQALRKLTGIVNKSKCIVVFINQLRDKVGVIFGSPETTTGGRALKFYA
                     SIRLDVRKVDTLKQNNDFVGNRTRVKVVKNKVAPPFKQAEFDIMYGEGISREGDILDL
                     AVEEDIIQKSGAWYSYEGNRLGQGRENAKEFLKQNPALCDEIENLIRQRHGLPLIPVV
                     ASEKEILENVSDNQENND"
     gene            6437..6919
                     /locus_tag="PLG49_09740"
     CDS             6437..6919
                     /locus_tag="PLG49_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOQ17626.1"
                     /translation="MDEVQDRDFLKAKNDAYKFLSYKMRTKKEVMEKLISKGYGEDTI
                     NAVIELLERYKYIDDEAFAYAYARDSIQLKRRGRHKLIYDLKNKGVSQEIIDKILTYT
                     DLNEMENVNILIYKKIGNKKIGDLDYKEKNKLYQFLIRRGFSYDIIQKALGNQKDIFE
                     "
BASE COUNT         2480 a          886 c         1490 g         2092 t
ORIGIN      
        1 ttaataaatt attaagtgta taatttaata tactaaggaa gtaatattaa tattataagg
       61 agggtttaat atgcaaaatt taagtcaatt gttgcaaact ggagattgga aaggagaaaa
      121 acatgtacct gttattcaca tccctcaaaa tgttaaagct ggagaaccaa tagaaataaa
      181 agtatctatt ggggatgaaa tcaagcatcc taatgaatta gaacatcata ttaagtggat
      241 taaactattt tatttcaaaa aagacggaaa gtttcctgtt gaaattgctt cctttgactt
      301 tgtatctcat ggggaaggcg gagtcattac agagcctgta gcttctgtta acgtaaaact
      361 aaatgattca ggttttattt atgccctggc ttattgtaat attcatggtc tttgggaaaa
      421 taaagaagag attgttttcg aataattggt acggcattgc cgtacttttt tatttgattt
      481 aaaggcacga attaggtagt atataattat agcatataga aatatcagca aataataagg
      541 agtgacctat gtttacgaat tttatacaga atctttatcc catttttata gaagaagatt
      601 ttgaattgtt atctgaaggt gaagaatttg atattttttc agatgaaaac gaaatgatcc
      661 gctttgcaaa aagagaaaat acaatgcttt atattgttaa tatatggaat atggaaaagg
      721 ttgatttaga tacttttatc tttagaaaaa atgcttttaa gaaaaagtta gagcaggtgt
      781 ttgaagaact tcactgcagt tatcttattg tcttgaattt attggtgacg gatgaggaga
      841 ttccctttat caagccggaa gagtttcaac ccaataagga aatttataca atatcctgga
      901 ttattgattt gcagtataaa aaactgatta tacctaaaaa ggaaattgac gatgttctta
      961 atatcagaga gttaattcac aaagtttttc ggataattga cccgtctttg gaaaaagaag
     1021 cagatgattt gataacaaga aagaatgaga ggaaaaaaag atatttttta aggcaaagaa
     1081 cgaataatac ttttttgacc tatgttttta ttatccttaa tgtaatcata tggcttttaa
     1141 tggagcttaa tggtggttca gaagatatta atactttatt attatttgga gcaaatgagc
     1201 cttttttagt catagaaaga cagcagtact ggagattaat tacatccacc tttttacaca
     1261 ttggaattag tcatttattg tacaatactt ttgcccttta tatttttgga agcaggatag
     1321 aaaaatatta tgggaaagta gcttttttaa ttatttattt tatttcaggt atgattggca
     1381 gtatggctag tttaatattt agtgataccg tttcagcagg ggcttcaggt gctatttatg
     1441 gtttaatggg tgctttactg atgattgctt ataaaacgaa aaaagaagta gatggcttaa
     1501 atgcatttac gattttaatt atgattatgt tgggcgtagc atttggtttt cttatgccct
     1561 ttatcgataa tccagcccat ataggtggtt taattgcagg aatgatttca ggcagactca
     1621 ttctacaaaa aataaataag aaaaattatt gatttgaaat tttttcaaaa aaaacttgac
     1681 atgaatatta tagagttgta gtatatataa tatagacatc aactctatat atagatatat
     1741 tttagtatat ctcgggggtt gatcatatgc ttaagctttt ttatgtatgt ttttatattg
     1801 ggattattta taccgctgca acttttatcc ttggctatat ttttgatttc atgggtttag
     1861 atggggacat cggttttgac ggagattttt ttggctatgg gatatctccc ataaagccag
     1921 tggttattgc agtatttctg actatatttg ggggagtagg tattattgcg cacaaaaata
     1981 atttaacaag ccgactaagc cttattcttg cgcttgttat agcaatcagc atttcatttt
     2041 taattttccg ctttatttta gttcctctgc acaggatgca aagcaaggag gtggtcgaac
     2101 aaaatgaact aatcggtcat attgcaaaag ctgtattaac gattaaagaa gatgaatgtg
     2161 gtaaaatcgt ttatacaata aatggtaata catattcagc cccagcaagg tcagaaaata
     2221 aagaaaccat tgataaggga gaggaagtcg tcatagtgaa cattgaacat aatatatttt
     2281 atgtaaaaaa acttaaagag gaggggtttt aaatggattc tgctttaatg atagcggccg
     2341 ttgttgtggg ggttattatt cttttaatcc tttctattac catgatgtgg aaaaaagttc
     2401 cacaagataa ggccttggtc attactggtc taaagaaaag agttatatcc ggtggtgggg
     2461 gctttgttat ccctctttta gaaagaaccg acgttatttc cttagaaaat atgaaaattg
     2521 ttgtaagaac tgaaggcgct ttaactgaac agggtgttga tattattgct gacggcgttg
     2581 cagtgatcaa ggttaaatcg gatagagaat cgattttagc tgctgttgag caatttaata
     2641 caggttctga aaaaacaacc attaatttca taaaagacac ggctaatgac gttttagaag
     2701 gtaaattaag agaaattatt tctaaaatga ctgtggaaga aatttataaa gacagagaaa
     2761 aatttgcttc ccaggttcag gaagttgctg ctgtggactt ggctgaaatg ggtttagaaa
     2821 taaaagcctt taccattaga gatatttcag ataaaaacgg ctatctggaa gctttaggta
     2881 aaaaacgtat tgcagaagta aaaagagatg ccaatattgc tgaagcagaa gccaataaag
     2941 aaacaaaaat aaaaacagct gaagcggcgc gttttggtga agaagcaagg ataaaagcgg
     3001 aaacacaaat tgcagaagcg gcaaaagaaa aagaattaag aatccaggcc tttagaaaag
     3061 aacaggaaac agaaaaagcg aaagccgacc tggcttatga gattgaaacc agcaagattc
     3121 aacaagaagt tgaaaaagaa aaaatgcaaa ttcaaattgt aaagaaagaa aaagaaattg
     3181 aacttgctga gcaagaagcc atcagaaaag aaaaagaatt agaagcaacg attattaaac
     3241 aggcagatgc tgaaaagtac cgtgaagaaa aacgggcaga agccttaaga tatcaggaaa
     3301 tccaggaagc catggcaaaa gctgaagcca ttaaggcaat gggagctgca aaagctgaag
     3361 ccagccgctt agaagggatg gcagaagttg aaattatccg tgaaaagggt aaagctgaag
     3421 cagaagcaat gcttaaaaag gcagaagctt ataaattata taatgaggct gccgtaacag
     3481 aaatgattat cgataagata ccagaaattg caagagcgat tgcagaacct ctttcaaaag
     3541 ttgaaaagat tgtgattgta gattcaggca atggtgaagg caagggcgcc tcaaaaataa
     3601 caggttatgt aacagacatt atatccagcc tgccggaaac tgtggaagct ttaacaggcg
     3661 ttgatttaaa aaagattgta agtaacagag acttatttaa agagttagaa aataaaaaag
     3721 acattattga aaaagaagat caagtaaata atgagtaata aaaaagataa aagataaaag
     3781 aaaaatctta tgctaatttt tcttttatct tttatttttt tgggttaaaa taggattgaa
     3841 tttaaaggtt taggaggaag tttatgaacg cagagatctt ggcagttggt acagaattat
     3901 tgctagggaa tattgtcaat acaaatgccc agtatattgc aaaacgcctg gcagacatcg
     3961 ggatagatgt ttactttcag tcagttgttg gtgataattc tggtcgctta tataaagctt
     4021 ttgaattagc gctgcaaagg gcagatattg tcattacgac cggaggactg ggacctacac
     4081 aagatgattt gaccaaggaa attgctgctg aaatttcagg gaaaaagctt cttttagatg
     4141 ataaggcctt aaggatgata gaagaatatt ttagccggat gggaagaaca atgactgaaa
     4201 ataataaaaa acaagcctat attcctgaag cttccaaagt attttataat tacaggggaa
     4261 cagctcctgg ttgtgccatt gaaatggggg ggaagattat tattcttctt cctggtcctc
     4321 caagagaaat ggaagagatg tttgaaagaa gtgtcattcc ttatttatct caatttcaag
     4381 aaggtgtcct tgcttcaaaa attctgaggg tttgcggtgt tggagagagt aaagtagaag
     4441 aactgcttgc tgatttgctg gacagtcaga cgaatccgac gattgctcta tatgcaaaat
     4501 ttggagaagt tagcatccgt attacagcta aagctgagac agaggagaaa gcaaaggatt
     4561 taatctgtcc tgttgaaaat gaaatcagaa agattctggg taatcatata tatggagaag
     4621 gagaccagaa tttagaggac gtttttggag aattattaat taaacataaa aagagcatag
     4681 cagtagctga atcctgtacg ggaggtctgc ttgcggggcg attaattaat tatccaggaa
     4741 tttctgcagt ttttaaagaa ggagtcgtgg cttatagtaa tgaagctaaa ataagaagac
     4801 tgggagtaag tgaagctact ttaagtcaat atggtgcggt cagtaaagaa acagctcttg
     4861 aaatggcaaa gggaatatgc ataaaagcag gaaccagtat tggcatttcg acgacaggaa
     4921 ttgccggacc aggaggtggg agtattgaaa aacctgtagg tctggtatat gctggccttt
     4981 gcattgatgg agaactttat tcagtaaaat ttaattttac cggaaagcgg caggaaataa
     5041 gagaacggac tgttatttct gttttagact gggctagaaa gatactgata gaaaaattaa
     5101 attccgggaa attttaatct ttaatattga cggataaaaa gaaataagct aaaataagaa
     5161 cataagttcg attgtttatc tggcttgatt ttggatataa ctctaaagag attaaaatat
     5221 aatgaaaggt ggtgttcccg gaagatgcaa ggcatcgggc gggatcaata tgacaagtga
     5281 aagacaaaaa gctttggatg ctgcaataag tcaaatacaa aagcaatttg gtaaaggctc
     5341 aatcatgaaa cttggagaca ccatcgataa tgatctttca accatttcta cgggttcgtt
     5401 aagtctggat attgccttag gagtcggtgg tattccaaga ggaaggattg ttgaaatatt
     5461 tggacctgaa tcttcaggga aaacgactgt tgctcttcat atgattgcgc aaacacaaaa
     5521 agctggagga atagctgcat ttattgatgc agagcatgct ttggatcctt cgtatgctga
     5581 aaaattaggt gtcaatatca atgaattata tatttcccag cctgattatg gggaacaagc
     5641 tttagaaatt gctgaaacga tggtacgttc cggggctatt gacattgtta ttgtggactc
     5701 ggttgctgct ttagtcccta aagctgaaat cgaaggagaa atgggagaca gtcatatggg
     5761 tttacaagcc cgcttaatgt cccaggctct tagaaagtta acgggtattg taaataaatc
     5821 aaaatgtatt gtggttttta ttaatcagct tcgtgataaa gtcggtgtaa tctttggaag
     5881 tccggaaacg acgactggtg gtcgtgccct taaattctat gcatccatcc gtttagatgt
     5941 aagaaaagtt gataccttaa agcaaaataa cgattttgtt ggtaatcgta caagggtaaa
     6001 agtagttaaa aataaagttg cccctccttt taaacaggca gaatttgata ttatgtatgg
     6061 tgaaggcatt tcaagagaag gggatatatt ggatttggca gttgaagaag atattattca
     6121 aaaaagcggt gcatggtatt cctatgaagg aaaccgttta ggtcagggaa gagaaaacgc
     6181 aaaagaattt ttaaagcaaa atcctgcttt gtgtgatgag attgaaaacc tgattagaca
     6241 acgacatgga ttgcctttaa ttcctgttgt tgcaagcgag aaggaaattt tagagaatgt
     6301 ttcagataat caggaaaaca atgattaagt ctctttttta aaagagactc tctttttata
     6361 ttaaaatctt gaatttagaa cgagataaac agcttaacga attaaaaacg gaccagttgt
     6421 tttttggggt gatgggatgg atgaagttca ggacagagat tttttaaagg caaagaatga
     6481 tgcctataaa tttttatctt acaagatgag aacaaaaaaa gaagttatgg aaaaattaat
     6541 ttctaaagga tatggggaag acacaattaa tgcagttatt gagttattag aacgatataa
     6601 atacattgat gatgaagcct ttgcctatgc ttatgcccgg gatagtatcc agttgaaacg
     6661 aaggggaagg cataagttaa tttatgattt aaaaaataaa ggggttagtc aggaaattat
     6721 tgataaaatc cttacttata cagacttaaa tgagatggaa aatgtcaata ttttaattta
     6781 taaaaaaatc ggaaataaaa aaattgggga tttagattat aaagaaaaga ataaattgta
     6841 ccaatttttg ataagaagag gtttttctta tgatatcatt caaaaagcct taggaaatca
     6901 gaaagatatt ttcgaataaa ccattattaa atggatataa tgttcatt
//
LOCUS       DAOSUD010000097         6721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5472_length_6721_cov_22.863636, whole genome shotgun sequence.
ACCESSION   DAOSUD010000097 DAOSUD010000000
VERSION     DAOSUD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_6721_cov_22.863636"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..401)
                     /locus_tag="PLG49_09745"
     CDS             complement(18..401)
                     /locus_tag="PLG49_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ17627.1"
                     /translation="MADASLNKIFNFYQEVPLSSSLEPFISKNERVHFAVKTIRDVAV
                     FTDKRILIADKQGLTGKKIEYYSIPYKSIVTYSIETAGTFDLDSEIKLVLSGGISIEL
                     KFYKDKNMSNLLLKVYQIINDYIIQ"
     gene            653..1063
                     /locus_tag="PLG49_09750"
     CDS             653..1063
                     /locus_tag="PLG49_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ17628.1"
                     /translation="MGKQFGDRLRELRIERGMTQEELAREFNTGKASISHYESNRRLP
                     NANNINLFAEFFNVSIDYIMGKSDVRNPYITREQLVISPLSIKDKNQYQTFIEQIQLF
                     FMDEEIPEEDKEALFKDISKLFWEAKEICRQKTN"
     gene            complement(1144..1359)
                     /locus_tag="PLG49_09755"
                     /pseudo
     CDS             complement(1144..1359)
                     /locus_tag="PLG49_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1546..1989)
                     /locus_tag="PLG49_09760"
     CDS             complement(1546..1989)
                     /locus_tag="PLG49_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOQ17629.1"
                     /translation="MAYSDRELLARIIKCEAGGEGENGMKAVATVVMNRVRVPFGEYH
                     RVCQGDIRKVIYQEGQFDCVRSEIRGQLNPQTIWANPPEQIHYDIADWALSGNRLFTV
                     GYSLWYFNPFQPDCPYIFPRNGTGSFQVRVGDHCFYNPTELYAQT"
     gene            complement(2091..3287)
                     /locus_tag="PLG49_09765"
     CDS             complement(2091..3287)
                     /locus_tag="PLG49_09765"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17630.1"
                     /translation="MKRKKNISLLVLFSIFLLVVPVTAIAQKQTLESDENMAELYQVN
                     YSAENGTITANIKNDEKVPEGTEVTFTAQANEGYTFDYWAVKNENDELISKSTEMPFM
                     IIVNENIKVEAVIFEGNAENPTVDYVRKEVENFKDNYIWSYGKTVDQAYAEINVKIDE
                     LKEKLKLDSKEIFVTTVKHNNNGYVEVHIVSAIEGKNERVRIYSSECLKAIEAINAIN
                     ALKLTWDTDMSTTIKNYENNEELQNIVKKYKDEGLDIILVHDELTIYYVVKNDSKYVK
                     TGRYITNAVGGPEYELFKKTLQEEMMAKDLIWTPDLTVDELKKKIRNETKDIILNANK
                     QLREMNSKYRFQLDFRVNYYNNTQIVETLYAGVKIENSADQRAQYIPIANPKLNELIV
                     EGKNAD"
     gene            complement(3511..4002)
                     /locus_tag="PLG49_09770"
     CDS             complement(3511..4002)
                     /locus_tag="PLG49_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17631.1"
                     /translation="MIQYTIRPIKASEIDLLQDFLYEAIFQKDGEALLPKSIIHQPEI
                     KIYIEDFGRPHDLCLVAELDQKVAGAVWTRVFSNKAKSYAYIDERTPELAISLYKEYR
                     GQGIGTALMKKMLELLREKGYKQVSLSVQKDNYAFKMYKNLGFEIIKDLEKECIMIYH
                     LME"
     gene            complement(4023..5231)
                     /locus_tag="PLG49_09775"
     CDS             complement(4023..5231)
                     /locus_tag="PLG49_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOQ17632.1"
                     /translation="MMLIEGKHNTAKVFTDIIEESAIQQIKLLCDQEFTKGSKIRIMP
                     DVHTGAGCTIGTTMTIKDCIVPNLVGVDIGCGMEVVKIKNSYIELEKLDKLIHSKIPA
                     GFNIRKTPHKYADSIDLEKLRCKDEGKINLDRAIKSIGTLGGGNHFIEAGKDEAGQIY
                     IVIHSGSRHLGNEVAKFYQDQAYKSLNKNTSADIERLIAEYKAAGRQKEIQSAIAALR
                     AQVLTDIPKSLAYVSGQLMEDYLHDMELVQRFAYLNRKAMMEEIIKGMKLKIEDQFTT
                     IHNYIDTTNMILRKGAVSAQKGEKLLIPINMRDGSLICIGKGNEDWNYSAPHGAGRLM
                     SRNVAKNSFTVSEFKKEMKGIYTTTINKDTLDECPMAYKRMEDIVNNIQDTVEIVNRI
                     KPIYNFKAAE"
     gene            complement(5306..6586)
                     /locus_tag="PLG49_09780"
     CDS             complement(5306..6586)
                     /locus_tag="PLG49_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17633.1"
                     /translation="MSSKKRISFVMVITIILFMFPSVLSAEMTDLAEEAASIEIEMSE
                     VYKEIVEDNKNAEETDKNAAKEFVVNYSVKNGIISTSIASGQKVPAGTVLTFYVWPDE
                     GYVFDYWLVKDEEGKVLSKVKTGTLKYEIKADIIIEAVLFEGSAVNPTVSYARKAIEN
                     LNYTWPYGKTMGEVYKEITAKVNNLKEILNLDSKRINISVVEIYGAGRIEIQISSQVE
                     KKKEVVIIDSPEVIRLKEIFSEIDALNLTWDTDKTTTIMNLKNNEELQAIIEKYKEQG
                     INIEIHHDPLKVFLVAKNDSNFVKTGYYMARSIKSPEYEVFNDAMQEFYNKNFFWSED
                     LTIEEMREKIREESKDIVINANKRLRKINTSYRISNVFDETYYKDDNRTGIVTTLRPG
                     LELRISPNERYSSFFYLINPKAEELINAYENADN"
BASE COUNT         2077 a         1271 c          848 g         2525 t
ORIGIN      
        1 ataaaattaa tttaaactta ttgaatgata tagtcattaa taatttgata aacttttaat
       61 aaaagattac tcatattttt atccttataa aattttaatt caatactaat gcctcctgac
      121 aaaactaatt ttatctcaga atctaaatca aaagttcctg ctgtttctat agaatatgta
      181 acgatacttt tataaggaat cgaatagtat tcaattttct tgcctgtcaa accttgttta
      241 tctgctatca ggatccgttt atcagtaaat actgccacat ctctgatggt ttttactgca
      301 aaatgaaccc tttcattttt agaaataaac ggctcaagac ttgaagaaag aggaacttcc
      361 tgataaaaat taaagatttt gtttaaactt gcatcagcca taataatacc tccatcatta
      421 aattttctta aatcttctaa gtgtgtttta tatatattaa cgcattccct gactaaatat
      481 acatcttata ataaatttta tataaattac tataatttgt attatcccaa attgtttctt
      541 atttgtattt atggagttta atatagtgta tttacaaaat ataatataca tttaagaaat
      601 tggtatttat ttttgtaaca ttcattgata taataaaatg ggtgatgtgt atatgggtaa
      661 acaatttggt gatcgtttaa gggaattacg gatagaaaga ggaatgactc aggaagaact
      721 ggccagggaa tttaatactg gaaaagcttc tatttctcac tacgaaagta accgaagact
      781 tcctaacgct aataacatta atttatttgc agaattcttt aatgtttcaa tcgattacat
      841 catgggaaaa agtgacgtaa gaaacccata catcacaaga gaacagcttg taatatctcc
      901 tctttctata aaggataaaa atcagtacca aacctttata gaacaaatcc aactcttttt
      961 tatggatgaa gagattcctg aagaagataa agaagctctc ttcaaagata tctcaaaact
     1021 cttttgggaa gcaaaagaaa tatgcaggca aaaaactaat taacaaatga aaagatttct
     1081 agcaaaagct agaaatcttt ttaatttaag cattaaagat gtatcatttt aaatactcat
     1141 gcgttaataa taaattctta caaattttat ggaataaata tctgctaaca ccaggtcatc
     1201 ggtttccggc tctctcagaa taatataact gatcccgaca tctactaaag tcccttccct
     1261 gtctaccagc atattggtgc caattagaaa ctctactttt acatacttcc ctatctgctg
     1321 tcttaaataa ccttgagtat aattaatatc ttgttgtaca ggtgatcctg gttccatctc
     1381 aaaatctacc ggtgcaggac tttccctgtc tgctgctggt acagattttt tttggatgat
     1441 attataagct ggatacaatt ttttgtaacc tggtcccggc ccacaagtcc ttttgaattt
     1501 ataattcata tcattgcctc ctagctttat atttaaattt ttcgattatg tttgagcata
     1561 aagctcagtt ggattataaa agcaatgatc tccaaccctt acctgaaaac ttcctgtacc
     1621 attccttggg aaaatatatg gacaatctgg ctgaaaagga ttgaaatacc ataatgaata
     1681 gccaacggta aaaagacggt tgccggataa tgcccagtca gcaatatcat aatgaatttg
     1741 ttctggtgga tttgcccaaa tcgtttgggg attgagctgt cctctaattt ctgaccttac
     1801 acagtcaaat tgaccttcct gataaatgac ttttcttata tctccctgac atactctatg
     1861 atattctcca aaagggactc ttactctatt cataacaact gtcgccaccg ctttcatgcc
     1921 gttttctcct tctcctccag cttcacattt tataattctt gctaataatt ccctgtcaga
     1981 ataagccaca caagcacctc ctctttttaa cgtctactat aatttattca tttcactaga
     2041 tattgttaca tttactaaaa taataaaaca aaacaaaaaa cgcggatgaa ttaatccgcg
     2101 tttttacctt ccacaataag ctcattcaat ttaggatttg ctataggtat atactgagct
     2161 ctctgatcag ctgaattctc tatctttaca ccagcataca atgtttcaac tatctgtgta
     2221 ttattataat aatttacccg gaaatcaagt tgaaaacgat attttgaatt catttctctt
     2281 aactgcttat tagcatttaa aataatatct ttcgtttcat ttctaatttt ctttttcaat
     2341 tcatcaactg tcaaatccgg agtccagatt aaatcctttg ccatcatttc ttcttgtaaa
     2401 gtctttttaa acagttcata ttctggtcct cctacagcat ttgttatata tctgccagtc
     2461 tttacgtact tactgtcatt tttcactaca tagtaaattg ttaattcatc atggactaat
     2521 ataatatcaa gaccttcatc tttatatttt ttaacgatat tttgcagttc ctcattgttt
     2581 tcataatttt ttattgtagt actcatatct gtatcccagg taagtttcaa tgcatttatt
     2641 gcattaattg cttcgatggc ttttaaacat tcagaagaat aaatccttac tcgttcgttc
     2701 tttccttcga ttgccgaaac tatatgtact tccacatatc cattattatt atgctttaca
     2761 gtagttacaa atatctcttt actatcaagc ttaagtttct cttttaattc atcaatcttc
     2821 acattaattt ctgcataagc ctgatcgact gtttttccat atgaccaaat ataattatct
     2881 ttgaaatttt caacttcttt tctcacataa tcaaccgttg gattttctgc attcccttca
     2941 aatataaccg cttcaacttt tatattttca tttacaatga tcataaaagg catttctgta
     3001 cttttagata tgagttcgtc attttcattt tttactgccc aatagtcaaa tgtataacct
     3061 tcatttgcct gtgcagtaaa agtaacctcc gtgccttccg gtactttttc atcattttta
     3121 atatttgccg taatggtacc attttctgct gaataattta cctgatataa ttcagccatg
     3181 ttttcatcag attctaaggt ttgtttctgc gctatggcgg taacaggaac tactaaaaga
     3241 aagatagaaa ataaaactaa taagctaata tttttttttc ttttcatttc ttttcctccc
     3301 aaagtataat agtaatttca agtattatat aatacattta aaattgtttc aagtattttt
     3361 aataaattaa atactaaaat attctaatat ttttacaaaa aatacttaaa agattgacca
     3421 actccttttt tagaaaaata aaaacatcca gccatctaaa aatttacagc cggatgcttt
     3481 tatagcaata aaagagaaat taagcattca tcattccatt aaatgataaa tcataatgca
     3541 ctctttctct aagtctttaa tgatttcaaa acccaaattt ttatacatct taaatgcata
     3601 attatctttt tgcacagata aagacacttg tttataaccc ttttccctga gcaattctaa
     3661 cattttcttc atcaatgctg ttcctattcc ctgaccgcga tattccttat ataaggaaat
     3721 ggccaattca ggtgtcctct catctatata ggcataactt ttagccttgt tgctaaatac
     3781 cctggtccaa actgcacctg ccactttttg gtcaagttca gcaaccaggc ataaatcatg
     3841 aggtctgcca aagtcctcta tataaatctt tatttctggt tgatgaataa tagatttggg
     3901 taataaagct tctccgtcct tttgaaaaat tgcttcatag agaaagtcct gtaacaaatc
     3961 aatttccgat gctttaatgg gtcttatggt atattgaatc ataaaaaagc tcctttgaat
     4021 ccttattctg ctgctttaaa attataaata ggttttattc ggttaacaat ttctacagta
     4081 tcctggatat tgtttacgat atcctccatt cttttatagg ccatgggaca ttcatccaaa
     4141 gtatctttat taattgtagt cgtataaatc cccttcattt ctttcttaaa ttctgaaacc
     4201 gtaaaagaat ttttggcaac atttctggac attaatctgc ctgcaccatg tggagctgaa
     4261 taattccaat cttcgttgcc tttcccgata cagattaatg aaccgtctct catatttatc
     4321 ggaataagca gcttttcgcc tttttgggct gaaacagcac cttttctgag aatcatattg
     4381 gtcgtatcaa tataattatg aatggttgta aattggtctt caatctttag tttcattcct
     4441 tttatgattt cttccatcat agctttccga ttaagataag caaacctctg aactaattcc
     4501 atatcatgca gataatcctc catcaattgc cctgaaacat atgccagaga ttttgggata
     4561 tccgtcagaa cttgtgctct taaagctgct atagctgact ggatttcttt ttgcctgcct
     4621 gccgctttat attctgcgat taatctttca atatcggcac tggtattttt atttaatgat
     4681 ttatatgcct ggtcctgata gaattttgca acctcattgc ccaaatgtct tgaacctgaa
     4741 tgaatgacaa tatagatctg ccctgcttca tctttcccag cttcaatgaa atgattacct
     4801 cctccaagtg tcccaataga tttaatggct ctgtcaagat taattttccc ttcatcctta
     4861 caccttagtt tttctaaatc tatactgtca gcatatttgt gaggtgtctt tctgatatta
     4921 aaaccagcag gaattttgga atgaatcaat ttgtccagtt tttctaattc tatataggaa
     4981 ttcttaattt ttaccacttc catgccacaa ccaatatcaa cacctactaa attgggtaca
     5041 atacagtctt ttatggtcat cgttgtccct atggtacaac ctgcccctgt atgaacatca
     5101 ggcatgattc tgatttttga acccttcgta aattcctgat cacataaaag ttttatctgt
     5161 tgaattgcac tttcttcaat aatatcggta aaaacctttg ccgtattgtg tttcccttcg
     5221 attaacatca tactatcccc tttttttata ctattgttat tataagacta atctgtaaaa
     5281 atagctataa aaataaatgc ggattttaat tatccgcatt ttcatacgca tttatcaact
     5341 cttcagcttt tggatttatc aagtaaaaaa atgagctata tctttcattt ggagaaatcc
     5401 ttaattctaa accaggtcgt aaagtcgtta ctatccctgt acgattatca tctttgtaat
     5461 acgtttcatc aaatacatta gaaatacgat agctggtatt aatttttctt agtcgtttat
     5521 tggcatttat aactatatcc tttgattctt cccttatctt ctccctcatt tcttcgatag
     5581 tcaagtcttc tgaccaaaag aaatttttat tgtaaaactc ttgcatagca tcgttaaaca
     5641 cttcatattc cggacttttt atacttctag ccatatagta tccggtttta acaaaattac
     5701 tatcattttt tgcaaccaag aaaactttca aaggatcatg gtgaatttca atattaattc
     5761 cctgctcttt atatttttct atgattgcct gaagttcttc gttgtttttt aagttcatta
     5821 ttgtcgttgt tttgtccgta tcccaagtta gatttaaagc atcaatttct gaaaaaattt
     5881 cttttaagcg tattacttca ggagaatcaa tgattacgac ttctttcttt ttttccactt
     5941 gagaagagat ttgtatttca atacgtccgg ctccatatat ttcaacaacg gatatgttta
     6001 ttcttttaga atctaaatta agtatttctt tcaaattatt gacttttgca gtgatttctt
     6061 tataaacctc acccattgtt tttccatagg gccatgtata attaagattt tcaatagctt
     6121 ttctggcata gctgaccgtt ggatttacag cactgccttc aaaaagaaca gcttcaatta
     6181 tgatgtcagc ttttatttca tatttcaatg ttcctgtctt taccttagat aaaacctttc
     6241 cctcttcatc ttttactaac caataatcaa aaacatatcc ttcatcaggc catacataaa
     6301 aagtaagtac cgtaccagca ggcacctttt gcccacttgc aatactggtt gaaatgatac
     6361 catttttaac agaataattt acaacaaatt ctttggctgc atttttatcc gtctcttcag
     6421 catttttgtt gtcctctaca atttctttat atacttcact catttctatt tctatggatg
     6481 cagcttcttc tgccaaatct gtcatttcag ctgataatac tgaaggaaac ataaaaagta
     6541 taatagtaat aaccataaca aaagatatcc tttttttact gctcattttt ctttctcctt
     6601 tattatttag taataataaa ataattcaac ttttttaaag aagtatataa caagtctctt
     6661 tctttataat ttattaaaat attaatttct ttagaacaaa ataaatatca atcccttttt
     6721 g
//
LOCUS       DAOSUD010000098         6678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5512_length_6678_cov_23.936887, whole genome shotgun sequence.
ACCESSION   DAOSUD010000098 DAOSUD010000000
VERSION     DAOSUD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5512_length_6678_cov_23.936887"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLG49_09785"
     CDS             complement(<1..223)
                     /locus_tag="PLG49_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17634.1"
                     /translation="MEKMIFCQSCGMPMNGNENLFATNADGSKNEDYCIYCYKDGAFT
                     ADITMNEMIEFCVPHVVKNNTGMTEEEARQ"
     gene            complement(536..1732)
                     /locus_tag="PLG49_09790"
     CDS             complement(536..1732)
                     /locus_tag="PLG49_09790"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ17635.1"
                     /translation="MLGGLLIYRKLLRDGMIQRVERFLSDLEHQSKSKILLQTEYYNI
                     ISRLLEYGFKNNVKSSLWKNYIFDLILEDENVFTLACERSRSIDIGIEMLAKRDLSIL
                     IKYININWDSLAIKLGIEPMKTVSIGKKDNLTVDEVYNMLLNYYHQNGSGILGKYKAF
                     RWEDKLVGIEYPDEVRLSDIVGYEHQKKTLTDNTEKFLEGKKANNVLLFGDRGTGKSS
                     SVKALLNEYAHRGLRLIELNKNQLVDYYKITSFVRNRNKYFIIFMDDLSFEEYETEYK
                     YLKALIDGSVEKKPDNLLIYATSNRRHLVKETWSDREKTEEVHISDSVQEKISLADRF
                     GIVLTYTSPSQEEYLKIVEALAEKHNLPVPKTELREKALQWQAWHNGRSGRTAKQFID
                     YLMGKL"
     gene            complement(1870..2592)
                     /locus_tag="PLG49_09795"
     CDS             complement(1870..2592)
                     /locus_tag="PLG49_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOQ17636.1"
                     /translation="MIQAKSYSTKNIVSCGLLAGISIILTRVFSYTIPIAGFPALRIG
                     FGDIPVIISGMLFGPVAGALTGTVSDVLGFMINPMGAFYIPGMTISAALRGFIPGLFY
                     WAVKNNKIKLNFHFINIIFSILMVLGAIYVPLSQNEGASTIALIFYGIIAFVFIMLPV
                     VLGIIIKTDDGLYSFDKILFVVTFCYYMISLLLNTYWLSLAYNKGFLIFLPGRIIAGL
                     VIIPLHAIIIFVLSRWFKYMKI"
     regulatory      complement(2670..2776)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            2955..3107
                     /locus_tag="PLG49_09800"
     CDS             2955..3107
                     /locus_tag="PLG49_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17637.1"
                     /translation="MKKSLNHYLISLLFLIFIPLLCIGCSRKKDDPYTAEEILQYANE
                     LYHQEF"
     assembly_gap    3476..3575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3576..3843
                     /locus_tag="PLG49_09805"
     CDS             <3576..3843
                     /locus_tag="PLG49_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17638.1"
                     /translation="FTYDKEISNPLADDSRIPNFVVYKKLKDHEKKSPYKTEVLVAEI
                     PYATFGENFEYEEILNRLQYEYIEKVKSGLFEDETIPEDMLSQP"
     gene            complement(3952..4656)
                     /locus_tag="PLG49_09810"
     CDS             complement(3952..4656)
                     /locus_tag="PLG49_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17639.1"
                     /translation="MKVSAVRNYKSPAYPTRAELKELPDYASEKSWNDKLLKTSLAMA
                     MTATLLNGCTNNGYPFDPFGGEVLSGEIAAPYILTEEEALKIIYEEASAAGIHLKADA
                     YSVDHLKLVADKNVIKSLYPGFSDTFIEDTENWFNEAIAQVNETEISFLVDGYDEEKK
                     IGIEYISDEDDELRMVNDFNLKALVNYSLEQSETDKYIKVISAEGYNSSREDAIEDLK
                     KQLQEFFDFLRAEGVL"
     gene            complement(4678..5808)
                     /locus_tag="PLG49_09815"
     CDS             complement(4678..5808)
                     /locus_tag="PLG49_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ17640.1"
                     /translation="MNISLHLTNRCNFSCKYCYVNKENPIDMSWDTAKKAIDMAVKMS
                     STKTGISFFGGEPLLRKDLIKEIVTYCEEIHQKTNIKFYYNMTSNGTLLDEDFLIYAR
                     QKRISIALSHDGIREAHDANRVTKNQKVSFDMVDEKCELLLKYNPYASVIMVLNPNTA
                     EYVYKSVVHLFNKGFKYIIMSMNYGAAWDDKALKVLKNQYKKLSKLYYEKSMANEKFF
                     LAPFDYKILSHVDNEKFKSSPCSLGKSHLTITADGSIYPCTQLINDENLLMGHVESGI
                     TRTAFYEDKEASKKPCLECAIKDRCNHTCSCINKQASGDIYKISPVLCAHEKMIIFIT
                     DALAAKLYKAKNKTFLNKHYNELYPFTSVIEDLLKKKEEKTQ"
     gene            complement(5825..6448)
                     /locus_tag="PLG49_09820"
     CDS             complement(5825..6448)
                     /locus_tag="PLG49_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17641.1"
                     /translation="MLLTACEKEEQGSYTIQNEETEKFSFFDKGKKLEKRRKEILESR
                     MLLMPTAIMQPYQLTEEEAWNIIERKAREYGLEFEHNSMTIDNIVLELDNYNNPEKDL
                     MPKEDLKQLKESHSLTLDGYDETKKVGIEYIALPTDLTKKELNLEELIYIYQLSGLLN
                     DALKKNHEDKHIKIIIGTTYNKKNVEEILEKQLDEFFTQLKEEKIIE"
BASE COUNT         2127 a         1243 c          902 g         2306 t
ORIGIN      
        1 tttgtcgggc ttcttcttct gtcattcccg tattattctt taccacatgc ggaacacaaa
       61 attcaatcat ttcattcatc gtaatgtctg cagtaaaagc cccgtcttta tagcaataaa
      121 tgcaatagtc ctcattctta ctaccatccg cattggtagc aaataagttt tcattcccgt
      181 tcataggcat tccacaactt tggcaaaaaa tcattttttc catatgcatc cccctaaaca
      241 attcattttt ttacgggttc attataaagc cctttaactg acaacagcct gtcatatttt
      301 aaaataaata ttttctgctt agaacttttt tagttatcag taactattct ggactcaata
      361 aatagttcat atcgaaacga gttcttgttc ctctcttgtt cttcttgcaa ttcttctaat
      421 tttttctttc ttttttttat tttgacactt gctttttact aatattcctt atctcatatt
      481 aaaagggaca gattcacatt ttaatacctg taatctgtcc ctgagccttt aaaaattata
      541 atttccccat taaataatca ataaattgct tagcagttct tcctgaacgg ccattatgcc
      601 atgcctgcca ctgaagtgcc ttttctctta attctgtttt tggtacaggc aggttatgtt
      661 tttcagcaag tgcttctact atcttcagat attcttcctg gctggggctg gtataagtaa
      721 gaacaatacc aaatcgatct gccagagaaa ttttttcctg aacagaatct gagatatgta
      781 cctcttccgt tttttccctg tcgctccagg tttctttaac taagtgtctt ctgttagaag
      841 tcgcataaat aagcagatta tctggcttct tttctacact gccatcaatc agagctttca
      901 aatacttata ctcggtttcg tattcttcga aagataaatc atccataaaa atgatgaaat
      961 acttatttct gtttcttaca aaagatgtaa ttttgtaata gtcaaccagt tgatttttat
     1021 ttaattctat taagcgaaga cccctgtggg catattcatt aagcagagct ttaacagaag
     1081 aagattttcc tgtacccctg tctccaaaaa gcaaaacatt gttagctttt ttaccttcta
     1141 aaaatttttc tgtattatct gttaaggttt tcttttgatg ttcataacca acaatatcac
     1201 ttagccttac ctcatcgggg tattcaatac caactaattt atcttcccat ctgaatgctt
     1261 tatatttgcc taatattcca cttccatttt ggtgatagta atttaacagc atattataaa
     1321 cttcatcaac ggttaaatta tcttttttcc ctatactcac agttttcata ggttctattc
     1381 ccagtttaat ggcaagagaa tcccagttaa tattgatata tttaattaaa atagacagat
     1441 ctcttttagc cagcatttcg ataccaatat caatgcttct gcttctttca caggcaaggg
     1501 taaaaacatt ctcatcttca agaattaaat caaaaatata attcttccac aaagaacttt
     1561 taacattatt tttaaatcca tattccaaca acctggaaat aatattgtag tattccgtct
     1621 gtagtaaaat cttactttta ctttgatgtt ccaaatctga aagaaagcgt tcaactcttt
     1681 gaatcatgcc atctcttaaa agtttacggt atattaataa accaccaagc ataataaccc
     1741 cccatttgag ataccaaagt atataaataa aagcctatga cgtcaactgg caagtacaaa
     1801 ttctagtacc ctgtgaaatg cacatcatag actttatctt tttgtatgag cattacaaaa
     1861 tgaaaaaact caaattttca tatatttaaa ccatctggac aaaacaaata taataatggc
     1921 gtgtaaaggg ataataacca aacctgcgat gattctgcca ggtaaaaaaa tcaaaaatcc
     1981 tttattataa gccaaagaca gccaataagt attcagcaac agtgatatca tataataaca
     2041 aaaggtaaca acaaacaaaa tcttgtcaaa tgaatagagg ccatcatctg tctttatgat
     2101 tattcccagc acaactggca acattataaa aacaaaggca ataatcccat aaaatattaa
     2161 agcaatagtg gatgcccctt cgttttggga taatggtaca tatatagcac caagaaccat
     2221 caaaatacta aagatgatat tgataaaatg aaaatttaac tttattttat tgtttttaac
     2281 agcccaataa aacaatccgg gaataaaacc cctcaaggcc gcacttatcg tcattcctgg
     2341 tatataaaaa gcgcccatgg ggttaatcat aaaacccaga acatcactga ccgttcctgt
     2401 taatgccccg gcaactggtc caaaaagcat tccacttatg ataacgggaa tatccccaaa
     2461 accaatgcga agggcaggaa atcccgcaat tggaattgta tatgaaaaaa ctcttgtcag
     2521 aataatgcta atcccagcaa gtagtccaca ggaaacaatg tttttcgtgc tgtaagactt
     2581 cgcttgtatc acgctctccc tcctttcctt agtatgatat gacagtagta gcgcacacca
     2641 cactaaagaa agaagctctt taatgtaatg tcagcggaag cgataaagta tcgcagccct
     2701 ggataaaacc caacaggctt tcgtttaagg gcaacttccc atcccttaac acttaacgca
     2761 ctttacctac tctgtcatta cataaaatat tctataacaa tctgtaataa aatgcaataa
     2821 aaatcactat ttaattaatg aactattctt tttaattgac gttaaaattt aattataata
     2881 aaataaaaaa ataataattt caaaaattat gaaatgctaa taaaaattta taagaattga
     2941 aaggataaaa agatatgaaa aaatctttaa accattacct catttcatta ttattcctta
     3001 tttttattcc attattatgt atcggttgca gccgcaaaaa agatgatcct tatactgcag
     3061 aagaaatctt acaatatgcc aatgaattat atcatcagga attttaaatc gtatcgatga
     3121 atgaagaata caaggaaaat tcttctgttt actacgaagc ggattatatt ttaagcccta
     3181 aagacaatta agatttcact tttagtattt catctaatgt tgaacaaacc aggcatctgg
     3241 ataggagtgc ttattttgga ccttatgaaa aaacaatctt ttcacactat tttaatgata
     3301 aaatgaatta tcacaaaaat gaaattgaag aattagctgc ttcctatgat gaatacattg
     3361 acgagtatga aagagctttc gtccaggaat tcaaggattt agaaaaagtt gctgactttt
     3421 tttataaagc caatcaaata tatgccttta catacgataa agaaatatcc aacccnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttta catacgataa agaaatatcc
     3601 aaccccttag cagatgatag tcgtattcca aattttgttg tatataaaaa gcttaaagac
     3661 catgaaaaaa aatctcctta caaaacagaa gtcctggtcg ctgaaatacc ttatgcaaca
     3721 tttggtgaaa attttgaata tgaagaaatc cttaatagac ttcaatatga atatattgaa
     3781 aaagtaaaat ctggtctttt tgaagatgaa accattccgg aagatatgct atcccagcct
     3841 tgaacttctt acgaaaaatc tttgcataaa atggcttatg ccatttcata aattcttgtt
     3901 aaaaaaacct tgctgataac cattaagatt atcaacaagg ttttttctgt tttataaaac
     3961 cccttcagct cttaaaaaat cgaagaattc ctgaagttgt tttttcagat cttctatagc
     4021 gtcctctctt gaactattat aaccttccgc agaaatcacc ttaatatatt tatctgtttc
     4081 actttgttct aaactatagt tgactaatgc tttcaaatta aagtcgttaa ccatccttaa
     4141 ttcgtcatct tcatcgctga tatactctat gccgattttt ttctcttcat cataaccatc
     4201 cacaagaaaa gaaatttctg tctcattgac ctgggctata gcctcattga accaattctc
     4261 tgtatcttct atgaaagtat ctgaaaatcc tgggtacaag cttttgataa catttttatc
     4321 cgcaaccagt tttaaatgat ctactgaata agcatctgct tttaaatgga tacctgcagc
     4381 actggcttct tcatagataa tttttaaggc ttcttcctct gtaaggatat aaggggctgc
     4441 aatttctcct gacaacactt ctccgccaaa aggatcaaaa ggatatccat tattagtaca
     4501 tccatttaac aaggtagctg tcattgccat ggctaaagac gtttttaaca acttatcatt
     4561 ccaacttttt tctgaggcat agtcaggtaa ttccttaagc tctgccctgg tgggataagc
     4621 tgggctttta tagtttctta cggcgctaac cttcatcgta ttctcctctc taaacattta
     4681 ctgggtcttt tcttcctttt tctttaacag atcttctatc acagaggtaa atggatacag
     4741 ctcgttataa tgtttattaa gaaatgtctt atttttagct ttatataatt tggcagcaag
     4801 ggcatcggta ataaaaataa tcattttttc atgagcgcac aatactggtg aaatcttata
     4861 gatatctccg cttgcctgtt tgttaataca actgcaggta tggttgcatc tgtccttaat
     4921 ggcacattct aaacatggtt ttttagaagc ttctttatct tcataaaaag cagttcttgt
     4981 aatacctgac tctacatgac ccattaaaag attttcgtca ttaataagct gggtacaagg
     5041 ataaatactc ccgtctgctg taatggttaa gtgactttta cctaaactgc agggactgct
     5101 tttaaatttt tcattatcaa catgagataa aattttataa tcaaaaggtg ccagaaaaaa
     5161 tttttcattg gccatggatt tttcataata taatttactt aattttttat actggttttt
     5221 caggactttt aatgctttat catcccaggc agcaccataa ttcatagaca taatgatata
     5281 tttaaagccc ttattaaaga gatgaacaac actcttgtaa acatattccg cagtattagg
     5341 atttaaaacc ataatgactg aagcataggg attgtatttt aaaagtaatt cacacttttc
     5401 atcaaccatg tcaaaggaaa ctttttgatt tttggtaacc ctgttggcat catgggcttc
     5461 tctgatgcca tcatggctta aagcaatgct gattcgtttt tgcctggcat aaataagaaa
     5521 atcttcatcc aataatgttc cattactggt catgttataa taaaacttta tatttgtttt
     5581 ttgatgtatt tcttcacaat aagttacaat ttccttaatt aagtcctttc tcagaagagg
     5641 ttctccacca aaaaaggata tccctgtctt agtggaagac atcttaaccg ccatatctat
     5701 agcctttttt gctgtatccc aactcatatc aatgggattt tccttattaa catagcagta
     5761 tttacaggag aaattgcagc ggtttgtaag atgaaggcta atattcataa aataatctcc
     5821 ttagtcattc aataattttt tcttctttta attgggtaaa aaattcatcc aactgcttct
     5881 caagaatttc ttcaacattt ttcttattat aagttgttcc aataatgatt ttgatatgtt
     5941 tatcttcatg gtttttttta agcgcatcat tcaacagacc tgataactga tagatataaa
     6001 ttaattcctc taaatttaat tcttttttag ttaaatcggt tggaagtgcg atatattcta
     6061 tacctacttt ttttgtttca tcatatccgt ccaaagtcag ggaatgactt tcttttagct
     6121 gctttaaatc ttctttaggc attaaatctt tttccggatt attataatta tccaattcaa
     6181 gaacgatatt gtctatcgtc atagaattat gctcaaattc aagaccatac tccctggctt
     6241 ttctttcaat aatattccag gcctcttctt ctgtaagctg ataaggctgc ataattgccg
     6301 tgggcatcaa aagcattctg gattccaaaa tctcttttct acgcttttct aattttttac
     6361 ccttgtcaaa aaatgaaaat ttttcagttt cttcattctg aatagtgtag gatccttgtt
     6421 cctccttttc gcaggctgtc agcagcatcg ataaaataag tcccaccgct gaagctttta
     6481 taactttttt attccattgc ctgcatattt gcattacaaa agaagaaaag aaggtttttt
     6541 taatatttca tttttgctgg gatagtcagg tctgtatttt ttattgactg gtttgatttt
     6601 catagatttc cgccaaattt ttctcattaa gataattata tattatagta aaaattttgt
     6661 caatccatca gtctttta
//
LOCUS       DAOSUD010000099         6567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5604_length_6567_cov_28.117475, whole genome shotgun sequence.
ACCESSION   DAOSUD010000099 DAOSUD010000000
VERSION     DAOSUD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_6567_cov_28.117475"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLG49_09825"
     CDS             <1..840
                     /locus_tag="PLG49_09825"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HOQ17642.1"
                     /translation="IDALWIGGKSMAEQYQKEKKSIFRRWWFWLLVILLIIIFGSMLG
                     DDDSNKEPVPQEQTKTQEDVNQEDKVEEEPQEKEPEKVQEPDEEVPDTAQTQQEEVSF
                     TNVFVQSDMGITLVYGEVTNNDTKAHSFTLKVSFYDKNNNLIGTAVGAVNDLNGGATK
                     IFAATGTDDVANAASYKVQVDTMIETKENQDELISFSNALVRSDLGITTVDVEATNND
                     TKSHTFTVNIGFYDKDNKLIGVATGAMNDLGPQETKTLSAIANGDLSTAASHKIFIDT
                     MVE"
     gene            complement(888..2990)
                     /locus_tag="PLG49_09830"
     CDS             complement(888..2990)
                     /locus_tag="PLG49_09830"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ17643.1"
                     /translation="MVKKELLLEGLDCANCALKIENKVKDIPGVKNAQLDFISQKLTL
                     EIHDENEIDTIIDKTTKIAKSIESNISVRSNLEKETEEKEENKIKSILLASSILLFLL
                     AYLLPVSDLFKNILFIVSYLFVGTEILLKAMKNILKGQIFDENFLMSIATVGAFLIGE
                     FPEAASVMIFYQIGEFFQDMAIDKSRKSIKALMDIRPDYANLKINHEIKRVAPEEVKV
                     GDLIIIKPGEKIPLDGIIIEGNSSLDTSALTGESLPREVKVNDEVLSGSINQEGLLTV
                     KVTQLFAESTVSKILNLVQNASTKKAPTENFITKFAKYYTPVIVAIAALLAVIPPLFI
                     PGASFSNWIYRALIFLVISCPCALVISIPLGFFGGIGRASKEGILIKGSNYLEALHNA
                     HTIIFDKTGTLTKGIFKVTEIQTRGNLSKEQLLEYAALAESFSNHPIALSIIQAYGKE
                     IDKSRIKQVDEISGYGLKVIIDDNELLLGNEKLMAKHNIKVDSNNTGTLVYIALNGNY
                     EGKIVISDEIKEDSPAAIEKLKALGIKKTIMLTGDNHSTAEIIARELKIDEFYSQLLP
                     QDKLEKLEELLKEKGAKEKLVFVGDGINDAPVLARADIGISMGTLGSDAAIEASDIVL
                     MTDEPSKIATAIRIAKKTQCIVWENIIFALGVKCFVLVLGALGIASMWAAVFADVGVA
                     LIAILNAIRILNTKKIYN"
     gene            complement(3008..3367)
                     /locus_tag="PLG49_09835"
     CDS             complement(3008..3367)
                     /locus_tag="PLG49_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ17644.1"
                     /translation="MANKDIKKCDCTIIHEDVVNKVKENMPPEENLYDLAELFKVFGD
                     STRIRILYALSASPMCVCDIAALLNMSQSAISHQLRILKQARLVKYKKEGKVVYYSLD
                     DDHVNQIFAHGLEHVKE"
     gene            complement(3521..3859)
                     /locus_tag="PLG49_09840"
     CDS             complement(3521..3859)
                     /locus_tag="PLG49_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17645.1"
                     /translation="MSCGYGYGYGYGGILNQLSRYIGETVTIYTASGGQSGSGFTGVL
                     IAVNPYYVTLVTGKGPAPSCPLGNSCGKYGHYPYGGGYGSYKYDGIYSTGSITNIPVY
                     MIVSFVHNTL"
     gene            complement(3899..4282)
                     /locus_tag="PLG49_09845"
     CDS             complement(3899..4282)
                     /locus_tag="PLG49_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17646.1"
                     /translation="MPQANIFSRGDLAALLSKHIGETVTIFTASGGQSGSGFTGVILG
                     VTDCCVRLLTRIGPPPGCSLSNSCANFNPGYGCGSHNPYGSYYPADKPGAISAGGWNG
                     YPVSTLGSIADIPIDTIVSFVHNAV"
     gene            4527..5579
                     /locus_tag="PLG49_09850"
     CDS             4527..5579
                     /locus_tag="PLG49_09850"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOQ17647.1"
                     /translation="METMIMKIDYDDMDLSIIKEAAQIIRNGGIVAFPTETVYGLGAN
                     ALDQESVKKIYLAKGRPSDNPLIVHISSIEELYPLVESIKLKAKILMDLFWPGPLTLI
                     FPKSKIIPDSITAGLSTVAVRMPDNKIARELIKYSGVPIAAPSANISGSPSPTKAEHV
                     IKDLNGKIDMILDGGSVRVGLESTVLDVTRDTPVILRPGGITKEMLEKAIGEVLIDSA
                     IISEKEFVDTPMAPGMKYKHYSPKAKLVIVEGEINKVIEKINSLAKENKEQKLKVGIM
                     ATEQTRENYQADLILSVGDRDKAETIAAKLFDTLRAFDEAGIDIIFSESFSYEEIGFA
                     IMNRLKKAAGYNILRV"
     gene            5594..6055
                     /locus_tag="PLG49_09855"
     CDS             5594..6055
                     /locus_tag="PLG49_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOQ17648.1"
                     /translation="MDCKKKIIFLCTGNTCRSPMAEALAKDEAQKNSLDLDIRSRGIA
                     VFFPSHASPHAIEAMKDYGIDLSRHISKGIEEEDIQNADIILTMTNAHKRALQDRFPE
                     VHGKIFTLKEFVGESGDIEDPFGGSLELYKNCAKTLSELIKKLMIKLNEMC"
     gene            6126..6554
                     /gene="rpiB"
                     /locus_tag="PLG49_09860"
     CDS             6126..6554
                     /gene="rpiB"
                     /locus_tag="PLG49_09860"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOQ17649.1"
                     /translation="MIAIGSDHGGYALKEEIVKYLKEQNIDYKDIGCYSEESCDYPQY
                     GKAVAQLVASGECEKGILICGTGIGISIAANKVKGIRAALCHDCFSAQAAREHNNANI
                     LAMGARVIGPGLAIKIVETFLATDFSNDERHVRRISQIEE"
BASE COUNT         2189 a         1073 c         1299 g         2006 t
ORIGIN      
        1 atagatgcat tatggatagg aggaaaaagt atggcagaac agtaccaaaa agagaaaaaa
       61 tctattttta gaagatggtg gttttggtta ttagtaatat tacttattat tatatttggg
      121 agtatgctgg gtgatgacga tagtaacaag gagcctgttc ctcaggaaca gacgaagact
      181 caggaagatg ttaatcaaga ggataaggtt gaagaagaac cacaagaaaa ggaacctgaa
      241 aaggtccaag agccagatga ggaagtacca gatactgccc agacacagca agaagaggtt
      301 tcatttacaa atgtattcgt tcagtccgat atgggaatta ctttggttta tggtgaagta
      361 accaataatg acacgaaggc tcattccttt acactaaagg ttagttttta tgataaaaat
      421 aataatttaa tcggtacggc tgtgggagca gtcaatgatt taaacggagg ggcaacaaaa
      481 atatttgctg caactgggac agatgatgta gccaatgctg caagttataa agtccaggtg
      541 gatacgatga ttgaaacaaa agaaaatcaa gatgagctga ttagtttttc caatgcgctt
      601 gtaaggtcgg atttaggcat tactacagta gatgtggaag ctaccaataa tgatacaaaa
      661 agccatacat ttacggtcaa tataggtttt tatgataaag acaacaaact aatcggcgta
      721 gcaacaggtg cgatgaatga tttaggtccc caggaaacta aaaccctttc tgcaattgcc
      781 aatggggatt tatcaacggc agcttcccat aaaatcttca tagataccat ggtagaataa
      841 aaaatggagc ctgtctttcc aaatggatag acaggctttt agcgtaatta attataaatt
      901 tttttggtat tcaggattct tatggcattt agaatagcaa tcaaggcaac gcccacatcg
      961 gcaaatacgg ctgcccacat actggcaata cccaaagctc cgagaactag tacaaagcat
     1021 tttacaccca gggcaaagat aatattttcc cagacaatac attgggtttt tttggcaatt
     1081 ctaatagcag ttgcaatttt tgaaggttca tcggtcatca ggactatatc ggaagcttct
     1141 atagcagcat cagaacctaa agtacccatt gaaataccga tatctgccct ggcaagaacg
     1201 ggggcatcgt tgattccatc gccgacaaag accagttttt cttttgctcc tttttctttt
     1261 aagagttctt ctaatttttc cagtttatcc tggggcaaaa gctgactgta aaattcatca
     1321 attttaagtt cccgagcaat aatttcagca gtagaatgat tgtctcctgt taacatgatt
     1381 gtttttttaa tgcctaaagc ctttagtttt tctatggcag caggagagtc ttcttttatt
     1441 tcatcggaaa tgacaatctt tccttcataa ttgccattaa gagcaatata aacaagagtt
     1501 cctgtgttat ttgaatcaac tttaatatta tgcttagcca ttaatttttc attgcctaac
     1561 agaagttcgt tgtcatcgat gataacttta aggccatagc ctgaaatttc atccacttgc
     1621 tttattctgc ttttatcaat ttcttttcca taggcctgta taattgaaag agcaatggga
     1681 tgatttgaaa aactttcagc taaagcggca tattccagta actgttcttt tgataaattt
     1741 cccctggtct ggatttctgt aactttaaag attcctttcg ttaaagtccc tgttttatca
     1801 aatatgatgg tatgagcatt gtgaagggct tctaaataat tgctgccttt aatgagaata
     1861 ccttcttttg aggcccgacc tattcctcca aaaaatccaa ggggaatgga aataaccagg
     1921 gcgcagggac aagatataac aaggaaaatc agagcccggt aaatccagtt ggaaaagctt
     1981 gcacccggga taaaaagagg aggaattacg gctaaaaggg ctgcaatagc tacaattaca
     2041 ggcgtataat attttgcaaa tttagtgata aaattttcgg ttggtgcttt ttttgtactg
     2101 gcgttttgaa ctaagtttaa tattttggag acagtagatt cagcaaaaag ctgagtaact
     2161 ttaaccgtta acaagccttc ttgatttata gatccagaaa gcacttcatc attcactttc
     2221 acttctctgg ggagagattc tcctgttaaa gcggaggtat caaggctgga atttccttca
     2281 ataatgatac cgtcaagtgg aatcttttca ccaggtttta tgatgataag gtctccaact
     2341 tttacttctt caggagctac tcttttgatt tcgtgattga ttttcagatt agcataatct
     2401 gggcggatgt ccattaaggc tttaatggat tttcttgatt tatcaattgc catatcctga
     2461 aagaattctc caatttgata aaaaatcatt acagaagcag cttcaggaaa ttccccgatt
     2521 aaaaaggcac ctactgtggc aatagacatt aagaagtttt catcaaaaat ttgtccttta
     2581 aggatattct tcatagcttt cagcagaatt tctgttccta caaataaata gctaacaata
     2641 aaaagtatat ttttaaacaa atcagaaaca ggaagcaaat aagctaaaag aaaaagcaat
     2701 atactgcttg caagaaggat gcttttgatt ttattttctt ctttttcttc tgtttctttt
     2761 tctaaattgc tacggacaga aatattggat tctatagatt ttgcaatttt tgtagtttta
     2821 tctataatcg tatctatttc attttcatcg tggatttcca gtgtaagttt ttgactgata
     2881 aagtcaagct gggcattttt aaccccggga atatctttca ctttattttc aatttttaaa
     2941 gcacaatttg cgcaatctaa accttccagt aataattctt tttttaccat ataaacctcc
     3001 tgactaacta ttccttaacg tgttctaatc catgtgcaaa gatttgattg acatggtcat
     3061 catccaggga atagtaaacc acttttcctt cttttttata tttaactaat cttgcttgtt
     3121 taagaattct gagttgatga gagatagcag actgactcat attaagaagt gctgcaatat
     3181 cacagacaca catgggagaa gccgataagg cgtagagtat ccgtattctt gtactgtctc
     3241 caaagacttt aaacaattca gccaaatcat ataaattttc ttctgggggc atattttctt
     3301 taactttatt tacaacatct tcatgaataa tagtacaatc gcatttttta atatccttat
     3361 ttgccattga ttttctcctt tcaaacaaat atatgaataa ctgttcatat gtacatttat
     3421 attatattac cagacttata aaatgtcaaa aaaaatcttg aataaacaag atttttaaaa
     3481 tattaaaatt tgttaaaaga ggagggaatt tctcttatga ttaaagggtg ttatgaacaa
     3541 atgagacaat catgtatacg ggaatattgg ttattgaacc agtggaataa atgccatcat
     3601 atttgtagga accataacct cctccatagg gataatggcc gtatttaccg caactgtttc
     3661 caagaggaca agaaggtgcg ggaccctttc ctgttactaa ggttacataa taagggttaa
     3721 cagctatcag aacacctgta aaacctgagc cagactgacc gccgctggcc gtatagatgg
     3781 taactgtttc tccaatatag cggcttaatt gattaaggat tccaccatat ccatagccgt
     3841 atccgtatcc acaggacatt atttagcctc cttttactgg attaatcatt aaagattttt
     3901 ataccgcatt gtgaacgaaa gaaacaatgg tatctatagg aatatctgca atagagccca
     3961 gagtggaaac tgggtaaccg ttccatccac cagcagaaat tgcccctggt ttatcagccg
     4021 gataatagga accataagga ttatggctgc cacatccata gccaggattg aaatttgcac
     4081 aggagttact aagggaacag cctggaggag gaccaatacg ggtaagaagt cttacacagc
     4141 agtctgttac tccaagaata acacctgtaa agcccgaacc agattgacct ccgctggcag
     4201 taaagatggt aacagtttct ccgatgtgct ttgaaagaag agcagcaaga tctcctctgg
     4261 agaaaatatt agcttgcggc atatcattac ctcctttatt tgttgttata ctataatata
     4321 tgggggcttt tatcaaaagg ttacgaataa agtcaaaaaa ataatttttt tttataaaaa
     4381 tgtatttaaa caagttggga aagagctctt gggaaggggt aaaaggactt gctaaaaaat
     4441 gataagaata ttaaatatta agttgaattc tatgttacaa taggggtaaa ataaacgata
     4501 gaaagtgaca caagggaagc gaaaatatgg agacaatgat aatgaaaatt gattatgatg
     4561 atatggattt aagtattatt aaagaagctg ctcaaatcat cagaaatggg ggaattgttg
     4621 cttttcccac ggaaaccgtt tatggtttag gcgcaaatgc attggatcaa gaaagtgtta
     4681 aaaaaatata tcttgctaaa ggcaggcctt ctgataatcc actgatagtt catatttctt
     4741 caattgaaga actgtatcct ctggtagagt ctataaaatt aaaagcgaaa attttaatgg
     4801 acttattttg gccaggacct cttaccttaa ttttccctaa atccaagatt attcctgatt
     4861 ctattacggc aggactttca acggtcgctg tcagaatgcc ggataataag attgccagag
     4921 aattaattaa atattcaggc gttcctatag ctgcaccttc tgccaatatt tctggtagtc
     4981 ccagtccgac aaaggctgaa catgtgatta aggatttaaa cgggaaaatt gatatgattt
     5041 tagatggcgg aagtgtaagg gttggtcttg aatcaaccgt tttggatgtg accagggata
     5101 ctcctgtgat tttaagacct ggtggtatta caaaggaaat gttagaaaag gctatagggg
     5161 aagttttaat tgattctgca attatctctg aaaaagaatt tgttgacaca cctatggctc
     5221 caggcatgaa atataagcac tattcaccaa aggcaaagct tgttattgtg gaaggagaaa
     5281 tcaataaagt cattgaaaaa ataaattccc tggctaaaga aaataaagaa caaaaattaa
     5341 aggtaggaat catggcgaca gaacagacca gggaaaatta tcaggcggat ttgatattat
     5401 cggtcgggga cagagataaa gcagaaacca ttgcagcaaa gctgtttgac accttgagag
     5461 cttttgatga agcaggcatt gatattattt tttcagaaag tttttcttat gaagaaattg
     5521 gttttgccat tatgaacaga ctaaaaaagg cagccggcta taatatttta agagtctaaa
     5581 ggtggggtgc atggtggatt gtaagaagaa aataatattt ttatgtacag gtaatacttg
     5641 cagaagtcct atggcagagg ccttagcaaa agacgaagcc caaaaaaatt ctcttgattt
     5701 agatatccgt tcaagaggca ttgcagtatt ttttccttcc catgccagtc cccatgctat
     5761 tgaagcaatg aaagactatg gcattgattt aagcaggcat atatcaaaag ggatagaaga
     5821 agaagatatt caaaatgcag atattatttt aaccatgacc aatgcccaca aaagagcatt
     5881 gcaggacaga tttccagaag ttcatggaaa aattttcacc ttaaaagagt ttgttgggga
     5941 atcaggagat atagaagacc cttttggggg cagccttgaa ctttataaaa attgtgcaaa
     6001 aacgcttagt gaattgatta aaaagctgat gataaaatta aacgaaatgt gttaaattat
     6061 ggtttgctat gcggttatag aagtggtatt atttaataaa aattatagta atggagggct
     6121 agaatatgat agcaatagga agtgaccatg gaggatatgc tttaaaagaa gaaatcgtaa
     6181 aatacttaaa ggaacagaat atcgactata aagatatcgg ttgttattct gaagagtcct
     6241 gcgattatcc acaatatggt aaggcagttg ctcaacttgt tgcgagtggg gaatgtgaaa
     6301 aaggtatcct tatttgcggt acgggaatag gtatatcgat tgctgccaat aaagttaaag
     6361 gcatcagggc agctttatgt catgattgtt tttcagctca ggcggcaagg gagcataaca
     6421 atgcgaacat attagctatg ggcgcaagag ttataggtcc cggacttgca attaagattg
     6481 tagagacttt tttggctact gattttagta atgatgaaag acatgtaaga cgtatttcac
     6541 aaattgaaga ataataattg aaattat
//
LOCUS       DAOSUD010000100         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5694_length_6445_cov_23.719405, whole genome shotgun sequence.
ACCESSION   DAOSUD010000100 DAOSUD010000000
VERSION     DAOSUD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5694_length_6445_cov_23.719405"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1196
                     /locus_tag="PLG49_09865"
                     /pseudo
     CDS             376..1196
                     /locus_tag="PLG49_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1367..1807
                     /locus_tag="PLG49_09870"
     CDS             1367..1807
                     /locus_tag="PLG49_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17650.1"
                     /translation="MKKAIIISLYTVLGILIVMCIIVYKILFSVSVPVDVSFAETAYI
                     RAEEYGEMPEIYTQIKQEDLEFLKSIFNRTAWKETPSCPCNGVELIFKSEKKDLIIYP
                     ADDGCPTMRIEEDGKDYYFHITDEDNEKFKKMLEGYGIIYNFGI"
     gene            2002..2535
                     /locus_tag="PLG49_09875"
     CDS             2002..2535
                     /locus_tag="PLG49_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17651.1"
                     /translation="MYVNDFNQDGKEELAVILYVGSGTGVSIDELHIISLSEEEYIFT
                     ESENETIIPNPMYFKDIVYESDRYHSQLLKEIQLKSYYNSDELWVDVHIGDKKRSVKI
                     GPFEEKLTVKDDTPVLGNIVSFDVEEGRLKAKFALGLLFESWASPYYIGDIYPDVNYQ
                     MGEFSLENLYFSELEHE"
     gene            2776..2910
                     /locus_tag="PLG49_09880"
     CDS             2776..2910
                     /locus_tag="PLG49_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17652.1"
                     /translation="MKCFQDAWNDWLSTDNPYAIEDREMIETDNSRYMNLIGITGKLK
                     "
     gene            2997..3416
                     /locus_tag="PLG49_09885"
     CDS             2997..3416
                     /locus_tag="PLG49_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17653.1"
                     /translation="MNKRKIRIYMIIFSFALILFIIGGMRLIKNVETVNPKFPEKYDV
                     VIIDHGQECWIMDDKEDIERFIKASKRVSEHVGEAPDWYILRFVFDHKQVFSIPYDDI
                     SYSQEAADIINSYIHNKKRYFHYKEKEAVLPITLIII"
     gene            3943..5973
                     /locus_tag="PLG49_09890"
     CDS             3943..5973
                     /locus_tag="PLG49_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ17654.1"
                     /translation="MKKHQGKMSGIFWRIFATSMLCMIIPMLASVPVVSRVSRKYFQE
                     AASNALLSTAIEKRNQMELALSNIEKQAQVIANQPLLVDALYEASVHSKEPDEAVLKK
                     ISQNLEYNYELGEGLFENIFLMYRNRDIADGIGGKSVGWEDEVIGSAESLLVREARIS
                     PTTGRPVMTIVAPIKKNERHLGTIAMAIELSNVSEKIISSNSIDENLNNFFLNTSGLV
                     ISAEDPNYLLTLNFGEEPDLQDFYKNIEAKKTGVGFFTFEGVDYIAAYSHSSRYGMYF
                     ISYKPVKVYKGMLSTMNQLLQGVVLFAIIIATLIIYFSSKGLAKPILAVTEQAESLAQ
                     GNLFMKVPEKFLKRKDELGRLSNSFGVMILNLKTIIQQIADASNQVAASSQELYASGE
                     QVGKAAENVGNRIMEIAAGAEEQSAQIDSALRSLKSLIQQIDEVNEKTDHMEKTTGNI
                     IKDISRGSQSANESVNKINNLKMDTEEISKVIAELGHNSNQIGQIIELISGIAEQTNL
                     LALNAAIEAARAGEAGKGFSVVADEIRKLAEESADASDRIAELIVEIRKGVDNAVHMM
                     DNGIKSVDSSVEAIQENGDIFAAINKEAEQLKNIVADVTRSVRKMTESSLSFERTMQE
                     INNVSQDFALNSQDVSASSEEQIAVTEEIVTFSKSLATMAEELSALISHFKF"
     gene            6155..>6445
                     /locus_tag="PLG49_09895"
     CDS             6155..>6445
                     /locus_tag="PLG49_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ17655.1"
                     /translation="MQIEVKIDRSCTEPKVIIVTDKITDEINHLMKKLSDDSPQMLSG
                     FKDDMLEIIDESDIISVYACSGKVFDITEKGEYTLRLKLYEIEERLDKNYFVR"
BASE COUNT         2240 a          832 c         1346 g         2027 t
ORIGIN      
        1 ataatgtcat ggactattta aagaattcga tgcagaaaaa tgattttcta atggtgaatt
       61 taatggctgc accgaataag gaaggttttt ataaaaaatt tggttttatt gaaagaccta
      121 atgaaaatat gggtgcaggc atggttatgt atcttaagaa ggaataagaa aagtactgag
      181 aattagataa ggcgttgtcg tattattcag gcacagatta attgtttttg tatgagaaaa
      241 gtcacctttt gtcttaaaaa gataaaaagg tgacttttat ttgtgatgga tattgtttat
      301 tttttaattt ttacacagca tatcaaaggg tttgcttgaa taatattact taaggatttt
      361 ttcaaggggg atactatggg tcattatatt gatgtggatc acaaagtcag gctatttgca
      421 gaagatctaa atccacaggg taaaaaacca ttgttttttt acatggctgg ccggcaaatc
      481 ataatctgtt tgaataccag ttagacaaat tcccgggatg ggggtaccat tgtattggaa
      541 ttgatacaag aggttttggt aaatcggata agccctatta tggatacgat tacaatactt
      601 tatcagatga tcttagaact gtggtggaaa cattaggttt acatgatttt attttagcag
      661 ggcattctac gggcggtgcc atagctgcca ggtatatggg acgacataag ggattcgggg
      721 tatctaagct ggtccttatt gcagcggcgg taccaagttt aattaagctt ccaagttttc
      781 cttacggcgc agaaaaacag actgtcttag aaattattaa ggggacttat aatgaccggc
      841 cgcaaatgct tagggacttt gggaacaatt ttttctttca gcatacttca caggcttttt
      901 cagattggtt ttttaattta ggcttgcagg ctgcaggctg ggcgacggca gcaattgccc
      961 agacatggat aaaggaaacg ctttttacag atttagaagt gattcatgtg cccactttaa
     1021 ttgttcacgg tattcatgat aaaatagtgc cttttgaact tgctgaaata cagcataaaa
     1081 tgatccggac ttcaacgtta gtggctcttg aatattctgg tcatggatgt ttttatgatc
     1141 aaaaggacga attcaatact ttattaagaa aatttattga aaatcaaaac ggataatatg
     1201 atcttaaaag ctcctctaag gagctttttt gttgactaca aaacaatatt aaagtatcat
     1261 ttattataaa tattacgaaa attaaaagcc ctttaaattt aaggaaataa aaataaaggg
     1321 aaaacacgat tagagaaaca gattcatttc gtacggaggc gggtagatga aaaaagcaat
     1381 aatcatttca ttgtatacgg ttttaggtat tcttattgta atgtgtatta ttgtttataa
     1441 gatactgttt tctgtttcag ttcctgtaga tgtaagtttt gcagaaacag catatataag
     1501 agcagaggaa tatggagaaa tgccagaaat ttatacgcag attaagcagg aagatttaga
     1561 gtttttaaaa tctatattta ataggactgc atggaaagaa acaccaagtt gcccatgtaa
     1621 tggggtggag ttaattttta agtcagaaaa aaaagacctc atcatttatc ctgctgatga
     1681 tggctgcccc acaatgcgta tagaagaaga tggaaaagat tattattttc atataacaga
     1741 tgaggacaat gaaaaattta agaaaatgtt agaagggtat ggaatcatct ataattttgg
     1801 gatatagtct gaagtttctg cagatgagat gacaggaaaa tccagtgtct tagaaggtgc
     1861 cagatagtgt tatgaaggtg tttcagtagt aaaagaaaaa aagaatatgg tatcaaagat
     1921 cgtggggtta ttctatatgt aaataataag ggtcattact ttgattggag ctatttaacg
     1981 tccagattta ttcttcctga aatgtatgtt aatgatttta atcaggatgg aaaagaagaa
     2041 ttagcggtta ttttgtatgt tggttcaggg acaggcgtat cgattgatga actgcatatt
     2101 atttcgttat cagaagagga atatattttt acagaatctg agaatgaaac aatcattcca
     2161 aatcctatgt atttcaaaga tattgtttat gaaagtgacc gctatcattc acagcttctt
     2221 aaagaaatcc agttaaaatc ttattataat tctgatgagt tatgggtgga tgttcatatt
     2281 ggagataaga aacgttctgt aaaaataggt ccttttgaag aaaagctgac tgtaaaagat
     2341 gatacacctg ttttagggaa cattgtctct tttgatgtgg aagaaggcag attaaaagca
     2401 aaatttgctt taggcttatt atttgagtcc tgggcaagtc catattatat tggagatatt
     2461 tatcctgatg tcaattacca aatgggggaa ttcagcttag aaaatcttta tttttcagaa
     2521 ctagagcatg aatgattttt tttgtaattt aataaaagat attttgaact gcaatgaaga
     2581 taaagaatag ctaacataag tgttattaag tttgaaaagt taacattaag aatataaaaa
     2641 taaaattatt aaataaaatc ataatgtaat atggaggatt aagagtgaga agaagaagcc
     2701 ttagccacct ggcattcaat agattggtgg aggcctaaat ttgaaaatga actcaaggac
     2761 ttaacgattt gggagatgaa atgtttccag gatgcttgga atgactggct gtcaacagac
     2821 aatccttatg ccatagaaga tagagaaatg atagaaacgg ataatagcag atatatgaat
     2881 ctcattggca taacaggaaa attaaaatag tgtctgctat gattttagaa agtgttttag
     2941 agcagatgaa ggctgccctt ttttgagatt tgaatactga cgaataagga ggaaaaatga
     3001 ataaaaggaa aatcagaatt tatatgataa ttttttcttt tgctttaatc ctttttatca
     3061 ttgggggaat gcgtttaata aagaacgtag aaacagtcaa tcctaaattt cctgaaaaat
     3121 atgatgtggt tatcattgat catgggcagg aatgctggat aatggatgat aaagaggaca
     3181 ttgaaagatt tataaaggct tcaaagaggg tttcagagca tgttggagaa gctccggact
     3241 ggtatatttt aagatttgta tttgatcata aacaagtgtt ttcaattccc tatgatgata
     3301 tcagttattc ccaagaagcg gcagatatta ttaattctta tatccataac aaaaaaagat
     3361 attttcatta taaagagaaa gaggcagttt tgccgataac attgattatt atttagatta
     3421 tttaaggtct gattcaagga gagcttttat tttcgagaga gaactatccg attcagaatg
     3481 tgaaaaacat ggtttagtaa ggccatagtt caattaaaca gtcatagttt catattttat
     3541 atttcatatg aaaggctttt atctttcgcc agcattattg acttagagcg tcagaatttt
     3601 atgctgtcgt tggataagcc ttttttgttt attgatgtga gattggttgt gctttatttg
     3661 ctaaacagtg attaaataag tttagtctta aaatttataa caatataggc caaaagtatc
     3721 aattgaatag atcaaaaaaa tgtcgaacaa tataatatac taaatttaag caaaattata
     3781 aatatattaa aaagtaaaaa catctattag catcagggta cattcttgta aacgccaggt
     3841 tttactagaa attttcttgt aatgacgagc catttagtta cggtttaatt atcgagacca
     3901 ttttatgaat gagctttatc attattaagg aggcggtagg atatgaagaa gcatcagggg
     3961 aaaatgtcag gaattttttg gagaattttt gcaacatcta tgctttgtat gatcatacct
     4021 atgttagcca gtgtgcctgt agtaagccgt gtttcaagga agtattttca ggaagcagca
     4081 agtaatgctt tactaagtac tgcaattgaa aaaagaaatc aaatggaatt agccctttct
     4141 aatattgaaa aacaggcaca agttattgct aatcaacctt tattagtgga tgctttatat
     4201 gaagcttctg ttcattcaaa agagcccgat gaagcagttc taaagaagat atcccagaat
     4261 ctggaataca attatgaatt gggagaaggg ctttttgaaa atatatttct tatgtatagg
     4321 aatagagata ttgctgatgg tattggaggt aaatcggttg gctgggaaga tgaagttata
     4381 ggcagtgctg aaagtttact agttcgagaa gcaaggatat ctcctactac tggtcgtcct
     4441 gttatgacaa ttgttgcacc cataaaaaag aatgagaggc atctcgggac catagctatg
     4501 gccatagaat taagcaatgt atcggagaaa attatttcta gcaattctat agatgagaat
     4561 ttaaacaatt tttttttaaa tacttcgggc cttgtgattt cagctgagga tcccaactat
     4621 cttttgactt taaattttgg tgaagagcca gatttgcagg atttttataa aaatattgaa
     4681 gccaaaaaaa cgggggtagg cttttttact tttgaaggtg tcgattatat tgctgcttac
     4741 agccacagca gccgatatgg tatgtatttt atctcttata aacctgtaaa ggtctataag
     4801 ggtatgttaa gtaccatgaa ccagttactg caaggcgttg ttttatttgc cattatcata
     4861 gcaacgttaa tcatttactt ttcttcaaag ggtttggcaa aaccgatatt ggcggttact
     4921 gaacaggcag aatcgttagc tcagggcaac ttgtttatga aggtacctga aaagtttcta
     4981 aagcgaaaag atgagttggg aaggctgtct aattcttttg gtgtgatgat tcttaattta
     5041 aaaacaatca ttcagcagat agcagatgca tcgaatcaag ttgctgcatc aagccaggag
     5101 ttgtatgcct ctggtgagca ggttggtaag gcagcagaaa atgtgggcaa tagaatcatg
     5161 gaaatagcag ctggagcaga agaacaatca gcacaaattg attctgcttt aagaagttta
     5221 aaaagcttaa ttcagcagat tgatgaagta aatgagaaaa cagatcatat ggaaaaaacc
     5281 actggaaata taataaaaga tatttcaagg ggaagccagt cagcaaatga atcggttaat
     5341 aaaatcaata atctgaaaat ggatacagag gaaatttcga aggttatcgc tgagttggga
     5401 cataattcta atcaaatagg acaaattatt gagttgatta gtgggatagc agagcagacc
     5461 aatttacttg ccttaaatgc agccattgaa gcggcaagag ctggagaagc cggaaaagga
     5521 tttagtgttg tcgccgatga aattagaaaa ttggcagaag aatcagcaga tgccagcgac
     5581 aggatagctg aattaattgt agaaataaga aaaggtgtag ataatgctgt tcatatgatg
     5641 gataacggta ttaaatctgt agattccagt gtagaggcta ttcaggaaaa tggagatatt
     5701 tttgcagcaa ttaacaaaga agcggaacag ctcaaaaata ttgtggcgga tgttacaaga
     5761 agcgtaagaa aaatgaccga aagcagtcta agctttgaac gaacgatgca ggagatcaat
     5821 aatgttagcc aggattttgc cttaaattca caagatgttt cagcatccag tgaagaacaa
     5881 attgctgtca cagaagaaat tgtaactttc tcaaaatcac tggcaacgat ggctgaagaa
     5941 ttatctgctt taattagcca ctttaaattt taaaattcaa tataaaaatg ggtattaaag
     6001 gtcttgtatc tgagtgggat taatcataat cccactttgt tattatatag ctgttatctt
     6061 accatctgta gcataaaaaa aaccagctat aaaagaataa tggaaagtaa ttgttggttt
     6121 ttttataatg taattgcaga aagggggcag ctaaatgcaa attgaggtta aaatagaccg
     6181 ttcgtgtact gagccaaaag tgattattgt tacagataag attacagatg aaattaatca
     6241 tctcatgaaa aaattgtcag atgattcacc acaaatgctt tcaggcttta aggatgacat
     6301 gctggaaatt atagatgaat ccgatatcat ttctgtatat gcttgctcag gaaaggtttt
     6361 tgatattact gaaaagggtg agtatacttt acgacttaaa ctttatgaaa tagaagaacg
     6421 actggacaag aattattttg tacgg
//
LOCUS       DAOSUD010000101         6395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5738_length_6395_cov_24.442587, whole genome shotgun sequence.
ACCESSION   DAOSUD010000101 DAOSUD010000000
VERSION     DAOSUD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5738_length_6395_cov_24.442587"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(650..901)
                     /locus_tag="PLG49_09900"
     CDS             complement(650..901)
                     /locus_tag="PLG49_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17656.1"
                     /translation="MNEAPRVEFLSPKIKNVIQNDNIKKSAKIISEGQKQGTIKDGNP
                     VALASAFWAAIMGVCQIAIQKQDFIYPNPEWIVDIIRRG"
     gene            1259..1735
                     /locus_tag="PLG49_09905"
     CDS             1259..1735
                     /locus_tag="PLG49_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOQ17657.1"
                     /translation="MIKSTLILKISVFIMGIPVIFVCVLGIIEFISKGPFNPKYALIL
                     YPIFIVQYIAAIPFYFGLYQAYKILRLIDKGEAFSELTVLALKKIKYCAFTISGLYIL
                     VMPCYYFFAKLDDAPGILFINWIIIFTSIVIGFLGSVLQRLLKEAIEIKTENELTV"
     gene            1746..1958
                     /locus_tag="PLG49_09910"
     CDS             1746..1958
                     /locus_tag="PLG49_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ17658.1"
                     /translation="MAIIVNIDVMLAKRKMSVTELSEKVGITMANLSILKNGKAKAIR
                     FSTLEAICKALNCQPGDILEYKKDYK"
     gene            complement(1993..2859)
                     /locus_tag="PLG49_09915"
     CDS             complement(1993..2859)
                     /locus_tag="PLG49_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ17659.1"
                     /translation="MHEVIAKGILSFKNGMNIYRGCTHGCIYCDSRSKCYQMNHAFED
                     IEVKINAPELLEDVLRRKRKKCMISTGSMTDPYIPLEMNLQYMRKCLEIIEKYGFGIN
                     IITKSKRILRDLDLLKRINEKAKCVVSMTLTTYDDSLCKILEPNVSTTSERFETLMIL
                     KDHGIPTVVWLSPILPYINDKEENIRGILDYCLKAKVKGIICFGICLTLREGNREYFY
                     QKLDKYFPGLKARYIREFGNSYVLYSRNHHYLMNIIKRTCKENNIMFESEKIFSYLNE
                     FPKGYEQLSFDL"
     gene            complement(2900..3400)
                     /locus_tag="PLG49_09920"
     CDS             complement(2900..3400)
                     /locus_tag="PLG49_09920"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ17660.1"
                     /translation="MFLKKALNKLKGAKIVMKNKISKIQTNLWFDTEAEEAAKFYTSI
                     FKNAKINKIIRNGNTEGKVMTVQFELEGQEFVALNGGPQFKFTEAISFIVNCKTQEEI
                     DYYWEKLSENGDKKAQVCGWLKDKFGVSWQIVPDNLADMLNDPNREKSERVMKAMLQM
                     KKIDLN"
     gene            complement(3418..5730)
                     /locus_tag="PLG49_09925"
     CDS             complement(3418..5730)
                     /locus_tag="PLG49_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17661.1"
                     /translation="MCCLNEKKMKDFIQVASARQISKIWIFTHEIVYYWGLKSLEEIF
                     QTIDIFIPEIYEFVDIAKMKKRSYEIKKNRLVEKSVSNILPLGFNSFYIEDTIPEMVT
                     IRNEFRKAFVSVSSGIEKTEAWLYPYALKAEADANLEALIYFKSKGESLISSNKLSEI
                     WRKSLCKLVESYDFLHRNNDVIINKAKKKELSLVELAQIYKNTNYYFFQRTREVESDF
                     IFSTFFRAVEWFELHEFEPWINVYTQDVSRVFHSKIDHEYSLLTRLFYFCRSDLLIRK
                     TKFSLEALLHILCVGYIDDSKPWKIIRDKDTIVDYLPYASMLVFAWHRIKPTNINNEI
                     FNQALLLLLQTQLVSGAWPLTLNDKEESIFSTYLAMTALSVTKPNGYQRYVNNAKKWL
                     LSQQNEVGCWYIEGGPSVMINILCLEAIKLSEGSNLVTYNLQENTSNIKSNATIKDKQ
                     NIEEIKKYIHEFIRKNIYELNSLHKVFLAWLDTKEYETVNSDLLEDLKKNVSIIITTV
                     TNIETYMLHNSLMPIQGFNKIIRLFKNDNTYYIGKFGNYNIVNVVSGMGSYTVGGSAD
                     VLKNAIVHWEPKLIISLGIAFGIDIKKQKLGDVLISSTIIPYDKNSKIENGILILRDP
                     WFPQISTFLKDRIRNLAYFRNKRFRTHFGCIITGENLIDDPNFKEMILDATNTYNIIG
                     GEMEAYGVFKTASENQIRHCLTIKGVCDWAEGKNALSDDQDENTNIKEKLQLFAASNA
                     VYVCKKFFSEDYAFTDIGIKSENEKCIKSV"
     gene            complement(5957..6346)
                     /locus_tag="PLG49_09930"
     CDS             complement(5957..6346)
                     /locus_tag="PLG49_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17662.1"
                     /translation="MLFPEDTYEELYELYYSFLTNEKKHKEIGYLYQANNKFVAFINF
                     SIRYEYVNGTNTSPVLFIEALYVLEEYRKQGIARKLINHAENYAKENGIKQIASDCLI
                     DNTISELFHKSCGFKETERVIFFVKSI"
BASE COUNT         2207 a         1031 c          738 g         2419 t
ORIGIN      
        1 ttattttacc atttctttca ttcttctcca atctctatga ggggtgatta tgtatattat
       61 attcatttat tagtttattt tgtcactata aataaaatta cattaatcat cataataacg
      121 gtaacgacaa ttttaggaag tggtttaaca atgtagcgac taattaaata actaaaaatt
      181 attgtcataa tagaaacaat tccaaactta actataaccg gacttgtcca atcatttaat
      241 aataaatata atgtacaaga tacatattat aagaattttt tgctatcgga ctaattaaag
      301 gtaaaggttt attccaatat ttaaatgaag ctgcccaaaa cattcctaca aatgatagta
      361 tccataaagg atataatatt acatgaaaaa ttttatccac caataaggga ttggatactt
      421 cactaagatt tttaaaaact aacatattaa aaccaaacaa catcaataaa aaataataca
      481 ctcatcataa atacatcaca taaaaacata accactatag tttttatcat gaaatggcca
      541 aaaatctact gctgatgaat aactagcccc tgcatgaaaa attaaaacca agatgattat
      601 taaagaacgc aaattatcca tgaaatgtag acgatttgta ttcatttatc taaccccttc
      661 ttataatatc tacaatccat tcaggattag gataaataaa atcttgtttt tgaattgcga
      721 tttgacaaac tcccataatt gctgcccaaa acgctgaagc aagagctaca ggattaccat
      781 ctttaatagt tccttgtttt tggccttcag agattatctt tgctgatttt ttgatattat
      841 cattttgaat gacatttttt atcttaggtg ataaaaattc tacacgtgga gcttcattca
      901 taagcacaat tttgcgctat taagtattaa ttttgctgca ttactaaaaa actctaatgg
      961 ttcataagct gttttattta acaatgattt agatttttct atccctaaca ttaccaggtt
     1021 ttcatataaa ttctcttaga aggaaaataa ttaaacatta agcctttact aatacctgca
     1081 gtttgggcta tgtcggtaat ttttgtagcc gaaaaaccct tttcaacaaa caaatctagc
     1141 gcaatactaa gtatttgtta attaaatgtc aagacattat tgttttttaa taaattttta
     1201 ttgataatcg ataaaatatg tattataata ttattaaaaa taatgcgagg tgtaatttat
     1261 gataaaatca acactaatat taaaaatatc agttttcata atgggaattc cagttatttt
     1321 tgtatgtgta ttaggaatta tcgagtttat cagtaaagga ccatttaatc caaaatatgc
     1381 attaatcttg tatcctatat ttattgtaca atacatagca gcaatcccat tttacttcgg
     1441 cctttatcag gcgtataaaa ttctacgttt aattgataaa ggcgaagctt tctctgaatt
     1501 aacagtactg gctttaaaga aaattaaata ttgtgctttt acgattagtg gattatatat
     1561 tttagtaatg ccatgttact actttttcgc caagttagat gacgcacctg gtatattatt
     1621 tattaattgg attataatct ttacctctat agttatcgga ttccttggtt ctgtacttca
     1681 aagactacta aaagaagcaa ttgagataaa aacagaaaat gaattaacgg tataaggtga
     1741 aaaagatggc aataattgtt aatattgatg tgatgttagc taagcgaaaa atgagtgtta
     1801 cagaactttc tgaaaaagtt ggaataacga tggcaaatct ttctatttta aaaaatggaa
     1861 aagctaaagc aattcgcttt tccacattag aagctatttg taaagcatta aattgtcaac
     1921 caggcgatat attagaatat aaaaaagatt ataaataata aaaacctccc taattgggag
     1981 gtttaaatca ttttataaat cgaaacttaa ttgttcatat cctttaggaa attcgtttaa
     2041 ataagaaaaa attttttcgc tttcaaacat aatattattt tccttacaag ttctttttat
     2101 gatattcatt aaataatgat gattcctact atataacaca taagagttac caaattctct
     2161 tatatatctt gcttttaatc ctgggaagta tttatctagt ttttgataaa aatattcacg
     2221 atttccttct cttaatgtta aacaaatacc aaaacatata atacctttaa ctttagcttt
     2281 gaggcaataa tccagtatac cacgaatatt ttcttcttta tcattgatat atggtagtat
     2341 gggactaagc catactacag taggaattcc atgatctttt aaaatcatta atgtttcaaa
     2401 tctttcactt gtcgttgata cattaggctc taatatttta caaagtgaat catcatatgt
     2461 tgttaatgtc attgacacaa cacacttagc tttttcatta atccgtttta ataaatctaa
     2521 atcacgtaat attcgctttg atttagttat tatattaatg ccaaaaccat atttttcaat
     2581 aatttctaaa cattttctca tatattgtaa attcatttct aaaggaatat aaggatctgt
     2641 catggaacct gtacttatca tacatttttt tctttttctt cttaaaacat cttcaagtaa
     2701 ttctggtgca ttaatcttta cttcaatatc ttcaaaagca tgattcattt gataacattt
     2761 actcctagaa tcacaataaa tacaaccatg agtacaaccc cgatatatat tcatcccatt
     2821 tttaaatgat aagatgcctt tagctattac ttcatgcata acctaaccct ttcttttaac
     2881 ccttatacgc ttgttttaac taatttaaat cgattttttt catttggagc atggccttca
     2941 taactctttc tgatttttct cgattcggat catttaacat atctgctaaa ttatcaggaa
     3001 ctatttgcca ggaaacacca aatttatctt taagccaacc gcatacttga gcctttttat
     3061 ctccattttc agataatttt tcccaataat aatctatttc ttcttgagtc ttacaattta
     3121 cgataaatga aattgcttcc gtaaatttaa attgaggacc accatttaat gcgacaaact
     3181 cttgtccttc taattcaaat tgaacggtca ttacttttcc ttctgtattt ccattacgaa
     3241 tgattttatt aatctttgcg tttttgaata ttgaagtata aaacttagct gcttcctcag
     3301 cttctgtatc aaaccacaaa ttagtttgaa tttttgagat tttatttttc attacaattt
     3361 tcgctccttt taatttattt aatgcttttt tcagaaacat ttttttacca accaaattta
     3421 tacagattta atacattttt cattctcact ctttatccca atgtcagtaa aagcatagtc
     3481 ttctgaaaaa aattttttac atacatacac tgcattactt gccgcaaata gttgcagttt
     3541 ttcttttata ttagtatttt catcttgatc atctgataaa gcattttttc cttcagccca
     3601 atcacaaacc ccttttatag ttaggcagtg acgtatttga ttttcacttg ctgttttaaa
     3661 aacaccatat gcctccattt ctccaccaat aatgttatag gtatttgttg catctaaaat
     3721 catctcttta aagtttggat catcaattaa attttcacct gttataatac atccaaaatg
     3781 tgttctaaat cttttatttc taaaataagc taggtttctt atcctatcct taagaaatgt
     3841 gctgatttgt ggaaaccaag gatctctaag tattaaaatg ccattttcaa tttttgaatt
     3901 tttatcataa ggaattattg tagatgaaat tagcacatca ccaagttttt gtttttttat
     3961 gtcaatacca aaggcaatgc caagtgatat aattagcttt ggttcccagt gaacaatggc
     4021 attcttaaga acatctgcgg aaccacctac agtgtatgaa cccattccag aaacaacatt
     4081 aactatattg taattcccaa actttcctat gtagtatgta ttatcgtttt taaataatct
     4141 tatgatttta ttaaagcctt gaattggcat tagactgtta tgcaacatat aagtttctat
     4201 attcgtaact gttgtaataa taatactaac attctttttc aaatcctcta ataaatcact
     4261 attaactgtt tcatattctt tagtatctaa ccatgctaaa aaaactttat gtaagctgtt
     4321 taattcatat atattttttc ttataaattc gtggatatat ttcttgattt cttcaatgtt
     4381 ttgtttatcc tttattgtag cattggattt tatattggat gtattttctt gtaaattata
     4441 tgtaactaag ttactacctt ctgataattt aattgcttct agacacaaaa tgttaatcat
     4501 aactgatggg ccaccttcaa tataccaaca accaacttca ttttgttgac ttaataacca
     4561 ttttttagcg ttattaacgt atctttgata cccgtttggt tttgttacag acaatgctgt
     4621 cattgctaag tatgtagaaa aaatgctttc ttctttatca ttcaatgtca gcggccaggc
     4681 tcctgaaact aattgagttt gtaataataa caataatgct tggttaaaga tctcgttatt
     4741 gatgtttgtt ggtttaatac gatgccaagc aaaaactaac atactagcat atggtaaata
     4801 atcaacaata gtatctttgt cccttataat tttccatggc ttagaatcat caatatatcc
     4861 tacacacaat atatgcaaaa gtgcttctaa gctaaacttc gttttgcgta ttaataaatc
     4921 cgatctacaa aaataaaaca atcgagttag taatgaatac tcatgatcta ttttactatg
     4981 aaatactctt gatacgtctt gtgtatagac attaatccat ggttcaaatt catgtagttc
     5041 aaaccactct accgccctaa aaaaagtaga aaaaataaaa tctgattcaa cttctcgagt
     5101 acgttgaaaa aaataataat tcgtattttt atatatttga gcaagctcaa ctaatgataa
     5161 ttcttttttc ttggctttat tgattattac atcattattt cgatgcaaaa aatcatagga
     5221 ttcaacaagt ttacacaatg attttctcca tatctcactt aatttattag aggatataag
     5281 tgattctccc ttgcttttaa aatatataag agcttcaaga ttagcatctg cttctgcttt
     5341 taacgcgtat gggtaaagcc atgcctctgt tttttcaatc ccagatgaga ccgatacaaa
     5401 agcttttcta aattcatttc gtatagtaac catttcagga atagtgtctt caatataaaa
     5461 cgaattaaac cctaaaggta atatattact gaccgatttt tccactaatc tatttttctt
     5521 tatttcatat gatctttttt tcattttagc tatatcgaca aattcataga tttctggaat
     5581 aaaaatatca attgtctgaa aaatctcctc taaagatttc aaaccccaat aatagacaat
     5641 ttcatgcgta aagatccaaa tttttgatat ttgtctagca cttgctacct gaataaaatc
     5701 tttcatcttt ttttcattca agcaacacat tctcttcacc tcacaaaccc catttcacaa
     5761 aatacttaaa tttgtaagtt gccattggta ttattactat ttactttaaa taataacact
     5821 cttacaaatt agcaaaatag aaatttaaat cttttaaaat ataatataaa ttttaaatac
     5881 atttatatat atatattaat ataatttaga tataataata attcaagaat cttttacaaa
     5941 aatatcttta aaatacttaa atacttttca caaaaaatat taccctttct gtttctttaa
     6001 aaccacaact cttatggaat aattcactaa ttgtattatc tattaaacaa tctgatgcga
     6061 tttgttttat accattttct ttcgcataat tttctgcatg atttattaat ttacgggcaa
     6121 ttccttgctt tcgatattct tctaaaacat ataatgcttc aataaataaa acaggtgaag
     6181 tattagtgcc attaacatat tcataacgaa tagaaaaatt gataaatgcg acaaacttat
     6241 tatttgcttg atataaataa ccgatttctt tatgtttttt ttcattagtt aaaaaactat
     6301 aatataattc atacaattct tcatatgtat cttcgggaaa taacataatc acccctcata
     6361 gagattggag aagaatgaaa gaaatggtaa aataa
//
LOCUS       DAOSUD010000102         6222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_5899_length_6222_cov_23.908383, whole genome shotgun sequence.
ACCESSION   DAOSUD010000102 DAOSUD010000000
VERSION     DAOSUD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_6222_cov_23.908383"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..1221
                     /locus_tag="PLG49_09935"
     CDS             493..1221
                     /locus_tag="PLG49_09935"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOQ17663.1"
                     /translation="MSLVQLKKLIKESSNIVFLGGAGVSTESNIPDFRSDKGIYSDKN
                     TQFAPETILSHSFFITNTELFYSFYKNKMIYKDAKPNAAHYALAKLEKMGKLRAVITQ
                     NIDGLHQAAGSINVLELHGSVHRNFCMDCGEFYNLDDVINSQETIPYCKKCKGIIKPD
                     VVLYEEPLNSDILEKAVGYVSESEILIVGGTSLLVYPAAGLVNYFRGKALVLINKSET
                     PYDSHADYVIHDSIGKVLTELADF"
     gene            1251..2474
                     /locus_tag="PLG49_09940"
     CDS             1251..2474
                     /locus_tag="PLG49_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HOQ17664.1"
                     /translation="MRILHTSDWHLGKHLEGYSRLEEQEKFIEELIEIIDRESVDLVI
                     IAGDIYDNGNPSAKAEKLFYSSLKRITKGGKIPALIIAGNHDNPDRLVAASPLAYEQG
                     IILLGTPNSKAVAGDYDYYRIVDAGEGYIEIEKNNEHLLALTLPYPSEKRLNEMISES
                     LEEEEQQKSYSQKIGSILENLSSKFREDTINIVVGHFYVAGGEVSDSERPVQLGGSYV
                     VEADVFPQKAQYIALGHLHRPQKVKGTDLEAYYSGSPIQYSKSEISYAKSVYIVDLLP
                     GQKAVVKEILLKNYKPIEVWKCDSIEEAIMKCKENSDKDVWVYLEIKTDRVLLQSEIK
                     EMRRLKKDIVEIVPVFNDEEEELEIEDIREKTLEELFVDFYYHQKQVKPTEEMIELFL
                     SIMNEGEEEDEAGTP"
     gene            2455..5979
                     /locus_tag="PLG49_09945"
     CDS             2455..5979
                     /locus_tag="PLG49_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ17665.1"
                     /translation="MKPVRLKIKGLNSYIEEQVIDFNRLTERGLFGIFGPTGSGKSTI
                     LDAIILALYGEIPRETKEFVNPEADSLYIYYEFSMKYGGERKNYVVERTFKRTEEGIR
                     KSKDARFYLKKDENEIEIIAESINLVTKEIEKTIGLKAADFTRSVVLPQGKFSEFLRL
                     TGAERRSMLERIFGLEKYGRFLGEKIKKTRDKKREELLNIESQLAVYGNITEEDYKAL
                     EESLKQQLEEEKQLRKEYDEINKQYEKYQSIWELQKELILYEDKLKELDEKEATYLNI
                     EKCLKRAEDAEKIKPHIDELEKIQAKAEETCKAMDMLKKRYDSLCKQKEETEGKYLEA
                     LNEKNIKHSLLLQKQANLDQAILLLEKKRLMESERNQLRQDYLKNSEAIKKEEEKEEK
                     IKEQLLEIEETLKKIEEEKEKIYIEPAFREEIQKAYEIEKEYLSLLTSQKETLEKVQQ
                     LAISIEKSKNQLEKWLKEKEEKAKNLTKVNEELKKLKEIPSSQELIFEKHKYIEALKS
                     QEKILTEQLKKKEAEQNRLIEISKALKEKEVQKENLFNEMEKLRKKLDQIKEEIKSLE
                     KTNMAAILASELKEGEACPVCGSTSHHPVHFDFDEEKYHDLLEQEKRYIQQFQEIEEK
                     YHDLVIKLAEIYNEKNQSETELKKTDDCVNEQEINKIKEKLSEEEKNLESLIFQISSI
                     EKALREKEKELSEIKEQLNMIEIEEARIQEGILRDEKQYKDLEQKSKELEEQVRKTTR
                     EYNAIKEKLQSDRIEEKIKQIIEWDKKRLSLEEKEKNLREEMKNKEKEKEELAKWISK
                     LNQDNAVIKQSGTEKSAMIKTWEEEIQKLSEGREPYQYKDEISKIIQGLIDKEAQLKE
                     VFEKIKAEEERVKESHIKLKTQWEALNQNQTEKNEKLSVLFQEYHFSNAEDVLKAWLE
                     KDKKKSLEEDLRNYKEMHNEVKNNIIRIRQRIGEDKISQEEWEKLKERKSNKAAELEM
                     KTKRIAVIQEKLNILIKDLEKVAELNRQKAKLDRIFSLLDELTRLVQGYKFVEYIASS
                     QLKYIAKEASKTLKQITMGRYALELDSTGNFVIRDDFNGGIRRSASTLSGGETFLTSL
                     SLALALSSQIQLKNASPLEFFFLDEGFGTLDSHLLDLVMSALERLHSQQLSVGIISHV
                     EELKNRIPVKLIVEPAEQGIRGSRVRIE"
BASE COUNT         2517 a          807 c         1241 g         1657 t
ORIGIN      
        1 atttttttac taagtgcatt tttaattccc ccttcttatt aaattattta aattgaatcc
       61 tatcatctgc aaaagtactt tttattgctt tgaccaaaag attttttgat ctataatctt
      121 tttttgcata atatatcgtt atcgccacaa aaactaattg aactataaga atacgggcaa
      181 catctgttct ttcggattat tagccaatat gattataacg tatttctact catctatttt
      241 tctactcatc tatgattctt tttataaatc tgaatataag ataataaaac aaaaataaag
      301 aggattttag ggttaaaagc aaaggcttat taacaaaggt ttattaacaa aggcagtaaa
      361 ataaatgaat accacttatt aagattaact ccattataga tataaaatta agaataatca
      421 ataaaattta acaaaattca tattgtagta taatatcagt atggaggaaa atatttaaaa
      481 gggggtaatc ctatgagcct ggttcagcta aaaaaactta taaaagaaag cagcaatatt
      541 gtctttttag gtggtgccgg tgtatcgact gagagtaata tacctgactt tagaagcgat
      601 aaaggaattt atagtgataa aaatacacaa tttgcgcctg aaaccatctt gagtcattct
      661 ttttttataa cgaacacaga gttattttat tctttttata agaacaaaat gatttataaa
      721 gatgcaaaac ctaatgcagc ccattatgct ctggctaaat tagaaaagat gggaaaatta
      781 agagcagtta ttacacaaaa tattgacggg cttcatcagg cagctggcag tataaatgtc
      841 ctggagttgc atggttctgt ccacagaaat ttttgtatgg attgtggtga attttataac
      901 ctggatgatg ttattaatag tcaggaaacg attccttatt gtaaaaaatg caaagggatt
      961 ataaagcctg atgtggtgct ttatgaagag ccattaaatt ctgatattct ggaaaaggca
     1021 gtaggctatg tgtctgagag tgaaatttta attgtaggtg ggacatctct tttagtatat
     1081 cctgcggctg gtctggttaa ctatttccgg gggaaggctt tggttttaat taataaatca
     1141 gaaacccctt atgacagtca tgcagattac gtgattcacg acagtattgg aaaggtatta
     1201 acggaactgg ctgattttta atacagcact ataataggag agatgatatt atgagaatac
     1261 ttcatacatc tgactggcat ttgggaaagc acctggaagg ttacagcaga ttggaagagc
     1321 aagaaaagtt tattgaagaa ttaatagaaa ttattgacag agaaagcgtt gatttagtca
     1381 ttattgccgg agacatctat gataatggta atccttctgc aaaagctgaa aaactttttt
     1441 attcttcttt aaagaggata acaaaaggag ggaagattcc tgctttaatc atagcaggca
     1501 atcatgataa tccggacagg ctggtggcag caagtcccct tgcctatgaa caggggatta
     1561 tcctgttggg aacacctaac agtaaagcag tagccgggga ttatgattac tatcgtattg
     1621 ttgatgcggg tgaaggttat atagaaatag aaaaaaacaa tgagcattta ctggctttaa
     1681 cccttcctta tcccagtgaa aaaaggctta atgaaatgat tagcgaaagc ctggaggaag
     1741 aagaacagca aaaaagttat tcgcaaaaaa taggcagtat attagagaat ctttcttcta
     1801 aattcaggga ggatacgatt aatattgtgg tcggtcattt ttatgtggcc gggggcgagg
     1861 ttagtgattc tgaacgacct gttcaattag gaggaagtta tgtggttgaa gcagatgtct
     1921 ttcctcagaa agctcagtac atagcccttg ggcatttgca tagacctcaa aaggtaaaag
     1981 gaacagattt agaagcctat tattcaggtt cccccattca atatagtaaa agtgaaatat
     2041 cttatgcaaa atctgtttat attgtagatc ttttgccagg acaaaaggca gtagtaaaag
     2101 aaattttatt aaaaaattac aaacccatag aagtatggaa atgtgacagc attgaagaag
     2161 ccataatgaa atgtaaagaa aacagcgata aagatgtatg ggtctatttg gagataaaaa
     2221 cggacagggt gctgttacaa tctgaaatca aggaaatgag gcgcttaaaa aaggatattg
     2281 ttgagattgt gcctgttttt aatgatgaag aagaagaact ggaaatagaa gatatccgag
     2341 aaaagacgct ggaagaatta tttgtagatt tttattatca tcaaaaacaa gtcaagccaa
     2401 cggaagaaat gatagaactt tttctttcca ttatgaatga aggagaggaa gaagatgaag
     2461 ccggtacgcc ttaaaataaa aggattaaat agctatatcg aagaacaagt catcgatttt
     2521 aaccgtttaa ccgaaagagg gctttttggg atttttggtc ctacaggaag cggaaagtcc
     2581 accattttag atgctattat ccttgccttg tatggggaaa tacccaggga aacaaaagaa
     2641 tttgttaatc cggaagctga cagcctttat atatattacg aattttctat gaaatatgga
     2701 ggtgaaagaa aaaattatgt tgtagaaaga acttttaaac gcacagaaga aggtattaga
     2761 aaatcaaaag atgccagatt ttatttaaag aaggatgaaa atgaaataga aataattgct
     2821 gaaagcatta atcttgtgac aaaagaaatc gaaaaaacca ttggtcttaa agcagcagat
     2881 tttacccgtt ctgttgtttt gccccaggga aaattcagtg aatttttaag gcttaccggg
     2941 gctgaaagac gcagtatgct agaacggatc tttgggttag aaaaatacgg aaggtttttg
     3001 ggagaaaaaa taaaaaagac acgagataaa aaaagagaag aattattgaa catcgaatct
     3061 cagcttgctg tatacggtaa tataacagaa gaagattata aagcactcga agaaagctta
     3121 aaacagcagt tggaagaaga aaagcaatta agaaaagaat atgatgaaat aaataagcaa
     3181 tatgaaaagt atcaaagtat ttgggaattg caaaaggaat taatcttata tgaggacaag
     3241 ctaaaagaat tggatgaaaa agaagcgact tatttaaata tagagaagtg tttaaaaaga
     3301 gcagaagatg ctgaaaaaat aaagcctcat atcgatgaac tggaaaaaat ccaagcaaaa
     3361 gcagaagaaa cctgtaaggc tatggacatg cttaaaaaac gctatgacag tctctgcaag
     3421 cagaaagaag aaacagaagg taagtatttg gaagctttaa atgagaaaaa tattaagcat
     3481 tctcttcttc ttcaaaaaca agccaatttg gatcaggcga ttttgctttt agaaaagaaa
     3541 aggctgatgg agtcagagag aaatcaatta agacaggact atttgaagaa cagtgaagct
     3601 ataaagaaag aagaagaaaa agaagaaaag ataaaagagc agcttttaga aattgaagaa
     3661 acattaaaga agatagaaga ggaaaaagaa aagatatata ttgagcctgc tttcagggaa
     3721 gaaatacaaa aggcttatga aatagaaaaa gaatatctga gtttattaac ctctcaaaaa
     3781 gaaacgctgg aaaaagttca gcaattagcc ataagtattg aaaaatctaa aaatcaatta
     3841 gaaaaatggt taaaggaaaa agaagaaaaa gcaaaaaatc ttacgaaagt caatgaagaa
     3901 ttgaaaaagt taaaggaaat accttccagt caggaattga tatttgaaaa acacaaatac
     3961 attgaggcat taaaaagtca ggaaaaaata ctcacagaac agttaaagaa aaaagaagca
     4021 gaacaaaacc gtctcattga gattagcaaa gctttaaaag aaaaagaagt tcaaaaagaa
     4081 aatttattca atgaaatgga aaagctaagg aaaaaattag atcaaataaa agaagaaata
     4141 aaaagtttgg aaaaaaccaa catggctgct atcctggctt ctgagcttaa agaaggagaa
     4201 gcttgtcctg tttgtggttc aacttctcat catccagttc attttgattt tgatgaagaa
     4261 aaatatcatg atttattaga gcaagaaaaa cgatacatcc agcaatttca ggagattgaa
     4321 gaaaaatatc atgatttggt tataaagctt gcagaaattt acaatgaaaa aaatcaatct
     4381 gaaacggaat tgaaaaagac agatgattgc gttaatgagc aagaaatcaa taaaataaag
     4441 gaaaaattat ctgaggaaga gaaaaattta gaatccctaa tatttcaaat cagcagtata
     4501 gaaaaagcac tgagagaaaa agagaaagag ctatcagaga taaaagaaca attaaatatg
     4561 atcgaaattg aagaagccag aatccaggaa ggaatcctca gagatgaaaa gcaatataaa
     4621 gatttagagc aaaagtctaa agaattagaa gaacaagttc gtaaaacgac cagggaatat
     4681 aatgcaatta aagaaaaatt gcagtcagac cgtatagaag aaaaaataaa gcaaataata
     4741 gaatgggata agaaaagact ttccctggag gaaaaggaaa aaaacctgag agaagaaatg
     4801 aaaaataaag aaaaagaaaa agaagagctt gcgaaatgga tttcaaaact taaccaggac
     4861 aatgcagtga ttaaacaaag cgggacagaa aagtcagcaa tgatcaagac ctgggaagaa
     4921 gaaatacaga agttgtctga aggaagagaa ccctatcagt acaaggatga aatatcaaaa
     4981 atcattcagg gattaataga taaagaagcc caattaaaag aagtatttga aaagataaaa
     5041 gctgaagaag aaagggtaaa agaaagtcat attaagctta aaacgcaatg ggaagctctg
     5101 aatcaaaatc agacagaaaa aaatgaaaaa ctgtctgtac tttttcagga atatcatttt
     5161 tccaatgcag aagatgttct taaagcctgg cttgaaaaag ataagaaaaa aagtctggaa
     5221 gaggatttaa gaaactataa agaaatgcat aatgaagtca aaaataatat cataagaatt
     5281 cgtcaaagaa ttggagaaga caaaatcagc caggaagaat gggaaaaatt aaaagaaaga
     5341 aaaagtaata aagcagcaga attagaaatg aaaacaaaaa ggatagctgt catccaggaa
     5401 aagctgaata tcttaataaa agatttagaa aaagtagcgg aattaaacag gcaaaaagca
     5461 aaactggaca gaatctttag tttgctggac gaattgacca gattagtgca aggctataaa
     5521 tttgttgaat atattgctag cagtcaatta aaatacattg caaaagaagc ttcaaaaact
     5581 ttaaaacaaa ttacgatggg cagatatgct ttagaattag acagtacagg taattttgtt
     5641 ataagggatg attttaatgg tggtatcagg cgttctgcca gcactttatc tggcggggaa
     5701 acttttctga cttctctttc cctggcctta gccctctcat cgcagattca attaaaaaat
     5761 gcatcaccgt tggaattctt tttcctggat gaaggttttg ggaccttgga cagtcattta
     5821 ctcgatcttg ttatgtctgc cctggaaaga cttcattctc aacagctctc agtaggtatt
     5881 atcagtcatg tggaagagct taaaaacagg attcctgtta agcttattgt tgaacctgct
     5941 gaacaaggga tcagaggtag cagggtaaga attgaataat cttctatctt ataaaaagtt
     6001 atgcctaaag tcagttttac tgcttttata aggataaaat aatctccgga tgtttataaa
     6061 gtgatgactg gcccaaaaga gtctgaccag atcaggaaca aatatttttt ggatatataa
     6121 gcatccggag gtaaaaaagc tgttaaatct gtctatgttt ttttcaatta cttgatatat
     6181 cgttataaaa aagggctata aataaaaatt aaaaaataaa tt
//
LOCUS       DAOSUD010000103         6016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6122_length_6016_cov_24.357658, whole genome shotgun sequence.
ACCESSION   DAOSUD010000103 DAOSUD010000000
VERSION     DAOSUD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6122_length_6016_cov_24.357658"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLG49_09950"
     CDS             <1..299
                     /locus_tag="PLG49_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17666.1"
                     /translation="SMPLLFAAGSDFFGNYIKILDKTHRHIDIDLIPYKLNRKIYFFP
                     QSFLALASYIKKAEFFKALEILAHIITAKEGIFHFKNPVPFLNYIRSLAKRNQS"
     gene            315..1112
                     /locus_tag="PLG49_09955"
     CDS             315..1112
                     /locus_tag="PLG49_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ17667.1"
                     /translation="MKVCLHIHSEYSHDSKIPVRKIIEYAKSLGYDVIAITDHNTVRG
                     SIEAKKYGDNKIKIIPGAEFSTQYGHILAYFIDDSIEKNTPRITKQQYDFYELVKNVK
                     HVNGLLILAHPFNSRLKEKMEILRYLDGIERYNSRIDSFYWRRKSEKLIRTLLNQENL
                     TYLAGTDAHSLEELKHCFVVTEDFELTTDSFKKALSKKNIIYYKPNVNFNIARAKFHN
                     TKHISPKFLMINGVRFLFGIIENIYNKTTGCRQYETLCFSKENQSKI"
     gene            1069..2403
                     /locus_tag="PLG49_09960"
     CDS             1069..2403
                     /locus_tag="PLG49_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ17668.1"
                     /translation="MKLYALARKINQRFKEKQKERAGRFLSPVRRIEKFSVNDDEKVV
                     SMTFDDGPMNMPINPLIDPKYEGWGLTRVLIDIMKEYDARGTFNIIGTTEYNYPDRPG
                     RLHKPDWSGIRHDHYPQFGKDKYAGAKNQKELIHMLLKNGHEVSNHGFKHVLFGKNNI
                     IYSRREHFSNIDEVVSDLKQLHDMIKKEFNYEMVMSRPPHYIDKMPDGFTSYDAYALI
                     KYDYLAASFDGGGWMPTVGDYKEDVRRMVEPIKRLLQEDENALKGHIIFQKDGYNMSL
                     MTPIASALKEQLEILKEYGYKVIPVRELKEKYCFEDFNDKTKNFEIARELDKKGFIIG
                     YKSNEFKPDKGLTIGEMLMMTLTKENYRNLLSRIYSSGRDKYLYLRQPYYIAFLHYNY
                     LDWMDRYNHPARDEDIRRFFYESFGITPKVKGNRIKRFEYLEALNEIKNLVK"
     gene            2601..4511
                     /locus_tag="PLG49_09965"
     CDS             2601..4511
                     /locus_tag="PLG49_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOQ17669.1"
                     /translation="MKKTRRLLGLFSILFILFNIYPEKLYAKDNILETELNIMINEYP
                     LKYHKKPIVKNGRILVPLRSIFEAMGIEVNWDPAERAVYAWKDDYFVKVVIDSTLTLR
                     NEEIYELDQSAIIYKDSTYVPLRFIGEAFGGEVIWDELSKTATVRTEFTTTPVIREFS
                     QMDIYIDNILTKSNYKPITYNGIEMIPIEPVLKAMGIKKYMDEENNEIVCIKNNDELR
                     INLTSNVVVLNNQYIYPVNIIFDYQGMLYLPLSLLEQAFKARTVWNSSANKVNIFNKE
                     LPFVLPVLSKESIEDAVIPQNAPTPVPKGNTRLMVSDNPENLNASSIPGETATLWQDA
                     IKEQEDYVEHIVFGYHKNQFDFPVAVGITIENLSKDNDIEIVNPKGVARYTSRDWGIY
                     DVGLKLAELSITDLLPPVLMKDLVVKAGETKVMDYFYVNTANLVSFQHQFVIKKKSGT
                     GNLNYVIRTVVSKKDEIPLESIKTEPLILDPAQRHPRGSWPFARLERELPLYEAGSSQ
                     IAYSISNGITDNIFSAETSFGKEYGTVGNIGHYAVINKIKIPVANYTGSSKTIQIRLN
                     PRGGRYGAVVKTNEGYFRVPEMSSKEVTKIIEYSLEDGQEEILEFEISNAGGSSLPIA
                     INIITLEEENYH"
     gene            4646..5767
                     /locus_tag="PLG49_09970"
     CDS             4646..5767
                     /locus_tag="PLG49_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOQ17670.1"
                     /translation="MLMEYLNALFFIFVAEMGDKTQILAMMFATKYKMSKVLLGVLIG
                     SFLNHGMAVLFGSLIGGVIPENVLQIIAGSAFIFFALWGLKEEDDEEEEKTSKKLGPV
                     FTVAAAFFIGELADKTQLTAITLAVNADYPFIVLLGTVSGMLLTSSLGIIIGSKLGDK
                     IPELALKMISFGVFLIFGTLKLYTAIPSQYINFYSISAFILFISISSVLLCRPILERR
                     KKGEKSLYLKTASSLQEYMKQMKDNIDDICLGENQCGKCLKEGCIIGYTREIIQEGME
                     DKEPCLEEAVHIEQFSMEKHFDETKLIESLCCTFRYLLDNYDDEHKRNIANAIRNNLE
                     LALFGEALEFNGDEESYLEQIKEKDISTWELILKSFQSV"
BASE COUNT         2168 a          793 c         1166 g         1889 t
ORIGIN      
        1 ggagtatgcc tctattattt gcagctggtt ctgatttttt tggcaattat attaagattt
       61 tagataaaac ccatagacat attgatattg atttgattcc atacaaatta aacaggaaaa
      121 tatatttttt tcctcaaagt tttttggctt tggcttctta tataaaaaaa gctgaatttt
      181 ttaaggcatt agaaatactt gcgcatatta tcaccgcaaa agaagggata tttcatttta
      241 aaaatccggt tccttttttg aattatatcc gttctttagc aaagagaaat caatcttaag
      301 ataggaagga ttttatgaaa gtttgcctgc atatacattc ggagtattct catgattcaa
      361 agattcctgt tagaaaaata atcgaatatg caaagagttt aggctatgat gttatagcta
      421 ttacagatca caatacggtc aggggtagta tagaagcaaa aaaatatgga gataataaga
      481 ttaaaattat tcctggagca gaattttcta cacaatatgg tcacatttta gcctatttca
      541 tagatgattc tattgaaaaa aatacgccca ggattactaa gcagcaatat gacttttatg
      601 aattggtgaa gaatgttaaa catgtaaacg gacttcttat tttagcgcat ccttttaaca
      661 gcagattaaa agaaaagatg gaaatattaa gatatctgga tggtattgaa agatataatt
      721 cccgtataga ttctttttac tggagaagga aatcagaaaa attaatacga actttattaa
      781 atcaagagaa tttgacttat cttgcgggta cggatgctca ttctttagaa gaattaaagc
      841 actgctttgt ggtaacagaa gactttgaat taactacaga ttcttttaaa aaagctttaa
      901 gcaaaaaaaa catcatatat tataagccta atgtgaattt taatattgcc agggcgaaat
      961 ttcataatac aaagcatatt agtccaaagt ttttaatgat aaatggcgtg agatttctct
     1021 ttggcattat tgagaatatc tataataaga caacagggtg caggcaatat gaaactttat
     1081 gctttagcaa ggaaaatcaa tcaaagattt aaagaaaagc aaaaagaaag agcaggcaga
     1141 tttttgtcgc ctgttagaag aattgaaaaa ttttcagtta atgatgatga aaaagtagtt
     1201 tctatgacct ttgatgatgg tccaatgaat atgcccatta atccacttat agacccaaaa
     1261 tacgaaggct ggggtcttac cagggtctta atagatatta tgaaagaata tgatgccagg
     1321 ggtactttta atattattgg aacgacggaa tataattatc cggacagacc aggcaggctt
     1381 cacaagcccg actggagtgg tatcaggcat gaccattatc ctcagtttgg aaaagataaa
     1441 tatgctgggg caaaaaatca aaaagaattg attcatatgt tgttaaagaa tggtcatgag
     1501 gtttcaaatc atggctttaa gcatgtttta tttggcaaaa acaatattat ttatagcagg
     1561 cgagaacatt tttctaatat cgatgaagtt gtatcagatt taaagcaatt acacgatatg
     1621 ataaagaaag aatttaatta tgaaatggtt atgtcaagac caccgcatta tattgataaa
     1681 atgccagatg gttttacatc ttatgacgct tatgctttaa ttaaatacga ttatctggca
     1741 gccagttttg atggtggggg ctggatgcct acggttggtg actataaaga agacgtcaga
     1801 aggatggttg agccaataaa aagattgctt caggaagatg aaaatgcctt aaaaggccat
     1861 attatctttc aaaaagatgg ttacaatatg tctttaatga cccctattgc ttcagcttta
     1921 aaagaacaac tggaaatttt aaaagaatat ggctataagg tgatacccgt cagggaacta
     1981 aaagaaaaat attgcttcga ggattttaac gataaaacaa aaaattttga aatagccagg
     2041 gagttagata aaaaaggttt tattattgga tataaaagta atgaatttaa accagataaa
     2101 ggattaacca ttggtgaaat gcttatgatg actttaacca aagaaaatta caggaatctg
     2161 ttaagcagga tttatagctc aggaagggat aaatatttat atttacgaca gccatattat
     2221 atagcttttc tgcattataa ttacttagat tggatggata gatataatca tccggcaaga
     2281 gacgaagaca tcaggagatt tttttatgaa agctttggaa tcactccaaa agtaaaggga
     2341 aacaggataa aacgctttga atatttagag gccttaaatg aaataaaaaa ccttgtaaaa
     2401 taaaccggaa aaccggtttt tatttttggc aatatttaag gaagaagtaa ataaatttaa
     2461 cgggtaaaat ttagttgaaa gtttaaataa ttttatagat tccaaagtca ttaagatgaa
     2521 ttggcaagtt ctaattttta tggtaacatg ttgacataac tagtttacaa ctaaaatgat
     2581 tattgctaga gagggatgcc atgaaaaaaa caagaagatt attaggactt ttttcaatac
     2641 tctttatact ttttaacatt tacccggaaa aactatatgc aaaggataat attttagaaa
     2701 ctgagttaaa tattatgatt aatgaatatc ccctaaaata tcataaaaaa cctatagtaa
     2761 aaaacggcag aattttggtt cctttacgtt ctatttttga agccatgggg attgaagtga
     2821 attgggatcc ggctgaaaga gcagtttatg catggaagga tgactatttt gttaaagttg
     2881 ttattgattc cactttaaca ttaagaaatg aggagattta tgaattagat cagtcagcaa
     2941 tcatttataa agacagtaca tatgtgcctt taaggtttat aggagaagct tttggtggag
     3001 aagtaatctg ggatgagtta agcaaaacag caactgtaag gacagaattt acgacgacac
     3061 ctgttataag ggaatttagt cagatggata tttacataga taatatatta acgaaaagca
     3121 actataagcc aattacatac aatgggatag aaatgattcc catagaaccc gttttaaaag
     3181 ccatgggtat aaaaaaatat atggatgaag aaaacaatga aatagtgtgt attaaaaata
     3241 acgatgaatt aagaattaat ttaaccagta atgttgtagt cctcaataat caatatattt
     3301 atcctgtaaa tatcattttt gattaccagg ggatgctgta tttgccgctt agcttacttg
     3361 aacaagcttt taaggcaagg acagtatgga atagttcagc aaacaaagtc aatattttta
     3421 ataaagaact tccgtttgta ttacctgtat tgagcaaaga aagtatcgaa gatgctgtta
     3481 ttccacaaaa tgctccaaca ccagttccaa aagggaatac aagattaatg gtgagtgata
     3541 atcccgagaa tttaaatgca agttctatac ctggtgaaac agctacactt tggcaagatg
     3601 caataaaaga gcaagaagat tatgtagagc acattgtgtt tggttaccat aagaatcaat
     3661 ttgattttcc agttgctgtg gggataacca ttgaaaattt atctaaggac aatgatatag
     3721 agatagtaaa ccctaaaggg gttgccagat atacaagcag agattggggt atatatgatg
     3781 tgggtctaaa acttgcagaa ttaagcatca ctgatttatt gcctccggtt ctgatgaaag
     3841 acttagttgt aaaagctggc gaaacaaaag tcatggatta tttttatgtc aacacagcaa
     3901 atttagttag ttttcaacat caatttgtca ttaagaaaaa atcagggact ggtaatctga
     3961 attatgtaat ccgtacagtg gtcagtaaaa aggatgaaat tcctttagaa tccattaaaa
     4021 cagaaccttt gattttggat cctgcccaaa ggcatccgag aggaagctgg ccatttgcta
     4081 gattggagag agaacttcct ttatatgaag cgggtagttc tcaaattgct tattccatat
     4141 ccaatggtat aacggataat attttttctg cagaaacgag ttttgggaaa gaatatggta
     4201 cggttggtaa tattggtcat tatgcagtga ttaacaagat aaaaattcca gttgccaatt
     4261 atactggttc ttcgaaaacc attcaaattc gtcttaatcc caggggtggc cgttatggtg
     4321 ccgtcgttaa gactaatgag ggttatttca gggtgcctga gatgagttct aaagaagtta
     4381 caaaaattat tgaatattcc ttggaagatg gtcaggagga gattcttgaa ttcgagataa
     4441 gcaacgcagg ggggtccagc cttcctatag cgataaatat cattaccctt gaggaagaga
     4501 attaccatta aaatgaaatc tatccacatt ttgtggataa aatgtggata actggtaaaa
     4561 aaagggtaat aaatattcac attgagattg caaattgatc aaatttgtga tattgttttt
     4621 ttgttataca aggagatgaa gaacaatgtt aatggaatat ttaaatgccc tgttttttat
     4681 ttttgtggct gaaatggggg ataagactca aattttggcc atgatgtttg cgacgaaata
     4741 taaaatgtct aaggtacttt taggggtttt aattggttca tttttgaacc atggcatggc
     4801 tgttttattt ggttctttaa ttgggggagt gatacctgaa aatgtattgc aaattatagc
     4861 aggttctgca tttattttct ttgctctgtg gggattaaaa gaagaggacg atgaggaaga
     4921 agaaaagacg tctaaaaagc tgggtcctgt ttttacagta gcagctgctt tttttattgg
     4981 agaattagcg gataaaacgc agttaacagc catcaccctg gcagtcaatg ctgactatcc
     5041 ttttattgtt ttattgggca cagtcagtgg tatgctttta accagtagtt taggaattat
     5101 tataggaagt aaactggggg ataaaattcc tgaattggct ttgaaaatga tttcctttgg
     5161 cgtattttta atttttggga ctctgaaact ttatactgcc attcccagtc aatatatcaa
     5221 tttttatagt ataagtgctt ttatactttt tataagtatt tccagcgtgc tgctttgcag
     5281 acctatactg gaaaggcgca aaaaaggcga aaagtcttta tatttaaaaa cagcttccag
     5341 tttgcaggaa tatatgaagc aaatgaaaga taacatcgac gatatctgtc tgggggaaaa
     5401 tcagtgcgga aaatgtttaa aggaaggctg tattataggt tatacaagag aaatcattca
     5461 ggaaggtatg gaagataagg aaccttgttt agaagaagca gttcatattg aacagttttc
     5521 catggaaaaa cactttgatg aaacaaaact aatagaaagt ttatgctgta cctttcgcta
     5581 tcttttagat aattatgatg atgaacataa aagaaatatt gccaatgcca taagaaataa
     5641 tctggagctg gccttatttg gtgaagccct tgaatttaat ggagatgagg aaagctattt
     5701 agagcagatt aaagaaaaag acatatctac ctgggaactc atattaaaaa gttttcagtc
     5761 tgtatagact gaaaactttt tttgtgccaa aaactacctc tttaaaatta ttaagaaaat
     5821 gttacaataa tataagatta gttaatactt ttgatataag ttttaataaa attgaaaagg
     5881 atgataataa tgaaaaaaat catagcaagc attgcctttg cattcatgat gattatattg
     5941 ccaacaaagg ttaatgcatc ttcctatatt atgcatacag taaaatccgg agaaacatta
     6001 aatagtatat caaaag
//
LOCUS       DAOSUD010000104         5967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6183_length_5967_cov_25.602673, whole genome shotgun sequence.
ACCESSION   DAOSUD010000104 DAOSUD010000000
VERSION     DAOSUD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_5967_cov_25.602673"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLG49_09975"
     CDS             <1..804
                     /locus_tag="PLG49_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOQ17671.1"
                     /translation="FIDYCKLVLYPDEKLENERNYLLLIKKGLKAQCGEMIKEDIKIQ
                     ITTAPKPYITKTFPEQSAKWVSIYPKITAEMSEELSDAIVTIDGVKQKVKIKGNKLEL
                     LPWEVLRANKEYEVQIQGVSKYGEKTDLYSLKFTTMPLKEDQLWVEVVLKEKHEVLIH
                     KGEEVIRRMPASGGKPEEPSVLGTFYIQDRGPSFFSPRFNEGALYWVRIVDQYLFHGI
                     PRDNHWNIIQSELEKIGKPASHGCIRLLDEDAKWFYENIPHGTMVIIHE"
     gene            905..2665
                     /locus_tag="PLG49_09980"
     CDS             905..2665
                     /locus_tag="PLG49_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOQ17672.1"
                     /translation="MNIVLSTLNAKYIHTSLALRSIRAYCKEFKENIKILEFTINHQE
                     NYILNEIFKAKPDILGFSCYIWNIEQTMDLVKSLKKVLPSATIILGGPEVSYDGEALL
                     KEHQEIDLIVLGEGEATFYDILKSFRDKKGDLADVEGIIYRINGKIIKTGERKLLDLN
                     EIAFPYEDFDALSNQIIYYESTRGCPYQCQYCLSSRDKKVRFLSLERVYADLQHFLDA
                     GVKQVKFVDRTFNCNKKHALSIWKYLMDKDNGKSNFHFEISADILDEEALELLSKARP
                     GLFQFEIGVQTTNRDVLKQIEREMDFDKLTAIVKKIKGYKNIHQHLDLIAGLPGENYP
                     SFKKSFNDVYLLYPEQLQLGFLKVLKGSKMRYNAEEYGLVYKDKAPYEILYTKELSYE
                     EVLKLKMIEEMLELYYNSGHFYYSIRYISSYFDSPFDFYEALAKYWEKKGYHHINHSK
                     ISLFTILLSFYQEKVKRNEEIFKELLKFDMHLLEKVKKFPEWMHSLNDKYKMEIRNFY
                     HNEENIKHFLPKLMGSSPKDISRKVHLELFSINITEIIKNIELGREIEEVREETVAVL
                     FDYLSKDEILGHANFYTVII"
     gene            2823..3476
                     /gene="nth"
                     /locus_tag="PLG49_09985"
     CDS             2823..3476
                     /gene="nth"
                     /locus_tag="PLG49_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ17673.1"
                     /translation="MKNKVKKILQLLDEHYPENTKCYLNYDTPWQLLIATILSAQCTD
                     DRVNLVTKDLFKKYTSLSDFANADIKELEQDIHSTGFYRNKAKNIILSARQLLEKYNG
                     QLPADIEKLTQLPGVGRKTANVILGNIFNIPSIVVDTHVKRVSNRLGLTTNTDPVKIE
                     FDLMKILPKSHWIRYNTQIIAHGRKICTARNPKCQACFIQPYCSYGQALIKGDLALH"
     gene            3493..3858
                     /locus_tag="PLG49_09990"
     CDS             3493..3858
                     /locus_tag="PLG49_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ17674.1"
                     /translation="MFVKKSYFKILGLGLLTGLVNGLFGAGGGTIVVPAMQKFLAVEE
                     HDSHANAIAVILPLSLMSTLIYSRHLKVDWNALFYVSLGGIIGGILGAKLLSKLSASW
                     LHKIFGFFMIVASIRMITG"
     gene            3855..4199
                     /locus_tag="PLG49_09995"
     CDS             3855..4199
                     /locus_tag="PLG49_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ17675.1"
                     /translation="MILFIIGLLSGLISGMGIGGGTVLIPALTIFLNFGQQIAQNINL
                     LYFIPTALAALVIHVKNKRIKKELITFLIAGGIIGAFAGSYIAVSLDAVILRKLFGFF
                     LLFMGISEVRKK"
     gene            4269..5117
                     /gene="mltG"
                     /locus_tag="PLG49_10000"
     CDS             4269..5117
                     /gene="mltG"
                     /locus_tag="PLG49_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ17676.1"
                     /translation="MNTIKNKLMDYGIWILLTLSILLLIIALILVYQTYDLLAEYNSF
                     NVSQKEIIPVIGTNVTKRKEIELVARRGDSYKKIENFLESNNILNPGELKRYLVNEKM
                     SLEGIVFEGSYHIPRPSSPEEVVSILTYPYKEWVETEYSKYEGIDKSPLEIITIASMI
                     EKEAALDEERPIIAGVIYNRLNKNMKLQIDATVIYALGEHKTRLSWEDLEVNSPYNTY
                     IVYGLPPTPICTPGSRSIEAALNPASHSYYYYVLAGYGNSHHNFSETYEEHLKNVQVY
                     LSSIEK"
     gene            complement(5167..5805)
                     /locus_tag="PLG49_10005"
     CDS             complement(5167..5805)
                     /locus_tag="PLG49_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ17677.1"
                     /translation="MKLPVFFSLFILFIIFYNIRLRKADKKIQKENEDFLEKERKAMF
                     VRRKPLDDLNYIKISPDGLPKLKEDDINSTYEKQAYQYQQEAFDYFNKPMLNLDGINN
                     TDLKLQYGPANLNTLIEYEQNFHRLQETLFKWGNALYKANRFDEAILVLEKAISIESD
                     LSKIYILLAELYTKTNNKDKLLDLNKITEKKARNNMIFRKVQTHIHNLLEEN"
BASE COUNT         2077 a          796 c         1094 g         2000 t
ORIGIN      
        1 tttatagatt attgtaagct tgttttatat ccggacgaaa aattagaaaa tgaaaggaat
       61 tatttactgc tgattaaaaa aggtttaaaa gcccagtgtg gagagatgat taaggaagat
      121 ataaagattc aaattactac agcaccaaag ccatatatta ccaagacctt tcctgagcaa
      181 tcagccaaat gggtttccat atatcctaag attacagcag agatgagtga agaattatcc
      241 gatgccattg ttacgataga cggtgtcaaa cagaaagtaa agataaaagg caataaactg
      301 gaacttttgc catgggaagt attaagggcc aataaagagt atgaagttca aattcaaggt
      361 gtatcaaaat atggggagaa gacagatctt tatagcttaa aatttacgac catgccttta
      421 aaagaagatc agttatgggt agaagttgtt ttaaaagaaa agcatgaagt tttaattcat
      481 aaaggggaag aagttataag aagaatgcct gcttcgggag gaaaaccaga agagccatca
      541 gttctgggaa cattttatat tcaggatagg ggaccaagct ttttctctcc cagatttaat
      601 gaaggcgcct tatattgggt cagaattgta gaccaatatt tattccatgg gatacctcgg
      661 gataatcatt ggaatattat tcagtcagag ttagaaaaaa tcggcaaacc agccagtcat
      721 ggatgtatca ggttattgga tgaagatgca aaatggtttt atgaaaatat tccccatggg
      781 accatggtga ttatacatga ataactgaat aaaaccggtg tctgcagctt atctgtttaa
      841 gttaactgac aggcgccgtt ttttgttata taattaagta aaactgttat aaggagcgtt
      901 tttcatgaat attgttttat ccaccctgaa tgcaaagtat atacatactt ctttagcttt
      961 aagaagtata agggcttatt gtaaagaatt taaggagaat attaaaattc ttgagtttac
     1021 gataaatcat caggaaaatt atattttaaa tgaaatattt aaagccaaac cagatatttt
     1081 gggtttttcc tgttatatat ggaatattga acaaactatg gatctggtca aaagcctgaa
     1141 gaaggtacta ccttctgcca cgattattct tggaggtccg gaggtttcct atgatggtga
     1201 agccttatta aaggagcatc aagaaataga cctgattgtc cttggagaag gtgaagctac
     1261 tttttatgat attcttaaat ctttcagaga taagaagggt gatttggctg atgtcgaggg
     1321 aataatttat agaataaatg ggaaaataat taaaactgga gaaagaaaac tattagattt
     1381 aaatgaaatc gcttttcctt atgaagattt tgatgctttg tctaatcaaa ttatatatta
     1441 tgaatcaacc aggggttgtc cttatcaatg tcaatactgc ttgtcttcca gggataaaaa
     1501 agtcaggttt ttatcattag aaagagtata tgctgattta cagcattttt tagatgcggg
     1561 cgtaaaacag gttaaatttg tagacaggac ttttaattgt aataaaaaac atgctttatc
     1621 catatggaaa tacttaatgg ataaggacaa tggaaagagc aattttcatt ttgaaatttc
     1681 agccgatatt ttagatgagg aagctttaga attattatcg aaagcaagac ctggtctttt
     1741 tcaatttgaa ataggggttc agacaaccaa cagggatgtc ttaaagcaga ttgaaagaga
     1801 aatggatttt gataaattaa cagcgattgt taaaaaaata aaaggttata agaatatcca
     1861 tcaacatctg gatttgattg ccgggcttcc tggggaaaac tatccatctt ttaaaaaatc
     1921 ctttaatgat gtttatttat tatatccaga acaattgcaa ctgggtttct taaaagtact
     1981 caagggttct aaaatgagat ataacgcgga ggaatatggc ctggtatata aagataaagc
     2041 gccttatgaa attctttata caaaagagct ttcttatgaa gaagtcttaa aattaaaaat
     2101 gattgaagaa atgttggagc tttattataa ttcgggacat ttttattaca gcatcagata
     2161 tattagttct tattttgatt cgccttttga tttttatgaa gctttagcaa agtattggga
     2221 gaaaaaaggt tatcatcata tcaatcacag caaaatatct ttatttacaa ttttactttc
     2281 cttttatcaa gaaaaagtaa aaaggaatga agaaatattt aaagaactgt taaaatttga
     2341 tatgcatctg cttgaaaagg ttaaaaaatt tccggaatgg atgcattcat tgaatgacaa
     2401 atataaaatg gaaataagga atttctatca taatgaagaa aatattaagc atttcttacc
     2461 caaattaatg ggaagttctc caaaagatat aagcaggaaa gttcacctgg agttattttc
     2521 cataaatatt acagagatta taaaaaatat agaacttgga agagaaatag aagaagttag
     2581 agaggaaact gttgctgttc tttttgatta tctttctaaa gatgagattt taggacatgc
     2641 taacttttat acagtcatta tatgataaac attatttccc aaataatgat atcatttccc
     2701 aggaaataat aggcatatat ttctattatt tcccaagtta tatatatttt gtttaaaaag
     2761 ataataaaat atttaattaa ttgtaattat gtcgaaaaat aaaagaaggg gttttccagg
     2821 caatgaaaaa caaggtcaaa aaaatcttac aactactcga tgaacattat cctgaaaata
     2881 cgaaatgcta tttaaactat gatacgccct ggcagcttct aattgcaaca attttaagtg
     2941 cccagtgtac agacgacagg gtaaatctgg tgacgaaaga tttatttaaa aaatatactt
     3001 ccttatctga ttttgcaaat gcagatataa aagaactgga acaagatatt cattctacag
     3061 gtttttacag gaataaggct aaaaatatta tcttatctgc cagacagctt cttgaaaagt
     3121 ataacggaca attgccagct gatatagaaa agttaactca attacccggg gtcggaagaa
     3181 aaacagccaa tgtaatttta ggcaatatct ttaatattcc cagcatcgtt gtggacacgc
     3241 atgttaaaag agtatctaac agactgggac ttacgaccaa tacagatcct gtaaagattg
     3301 aatttgattt aatgaagatt cttccaaaat ctcattggat tagatataat acacagatta
     3361 ttgctcatgg aagaaaaatt tgtaccgcaa gaaatccaaa atgtcaggcc tgtttcatac
     3421 agccttactg ttcttatggg caagcattga ttaaggggga tttggcgtta cattaatcgt
     3481 caaatcggag gaatttttgt gaaaaaaagt tattttaaga ttctgggatt aggtttatta
     3541 acaggattgg ttaatggtct ttttggtgca ggaggaggga ccattgttgt tcctgctatg
     3601 caaaagtttc tggcggttga agagcatgat tcccatgcca atgccattgc tgttatttta
     3661 cccttgtcac ttatgagtac attgatttat tcaagacatc ttaaagtgga ttggaatgct
     3721 cttttttatg tttctttggg cggcattatt ggaggaatcc ttggggctaa attattaagc
     3781 aaactttcag caagctggct tcataagatt tttggatttt ttatgattgt tgcttctata
     3841 aggatgatta caggatgata ttatttatca ttggtttgct ctcgggatta ataagtggga
     3901 tgggtattgg aggaggaacg gttttaattc ctgccctgac tattttttta aattttggtc
     3961 agcaaattgc tcaaaatatt aatttgcttt atttcatacc aactgccctt gcagctctgg
     4021 ttattcatgt aaaaaataaa agaattaaaa aagaattaat tactttcctg attgcaggtg
     4081 gtattattgg ggcatttgca ggttcttata ttgccgtttc attagatgct gtaattctta
     4141 gaaaattatt tggttttttt cttcttttca tggggataag tgaggtcagg aagaaataga
     4201 atgaatttac aagctaaaaa aactgtaata atataaagtt aaaaatttac ctttgttcag
     4261 ggagaaaaat gaatacaatt aaaaataaac tcatggatta tgggatatgg attttgctga
     4321 ccctgtctat attactttta attattgctt taatattggt ttatcagact tatgatttac
     4381 tggcagaata caattcattt aatgtaagtc agaaagaaat aattcctgtt attggaacca
     4441 atgtgacaaa aagaaaggaa atagaactgg tcgcaagaag aggagacagc tataaaaaaa
     4501 tagaaaattt tttagaaagt aataatatat taaatcctgg ggaactgaaa cgttatttag
     4561 tgaatgaaaa aatgagtttg gaagggattg ttttcgaagg cagttatcat atcccacggc
     4621 ctagttctcc agaagaagtg gtgagtattt taacctatcc ttataaggag tgggtggaaa
     4681 cagaatatag caaatatgaa ggaatcgaca aaagtccctt agaaattatt acgattgctt
     4741 caatgataga aaaagaagcg gccttagatg aggaaaggcc aattattgca ggagtcattt
     4801 ataaccgttt aaataagaat atgaagctgc aaattgatgc tacagtcatt tatgctttag
     4861 gagaacacaa gacaagatta tcctgggaag atttagaagt caattctcct tataatacat
     4921 atattgttta cgggcttccc ccgacaccga tttgcactcc gggttccaga tccattgaag
     4981 cagctctcaa tcctgcttct cattcatatt attattatgt tttagccgga tatggaaaca
     5041 gccatcacaa tttttctgaa acatacgaag agcatttaaa aaatgtacaa gtatatttgt
     5101 caagcataga aaaatagcat gaaattcata ctattttttt atgggaatat tgattttttc
     5161 aaaggattag ttttcctcta acaaattatg gatatgtgtt tggacttttc taaatatcat
     5221 gttattccta gctttttttt ctgttatttt gtttaaatct aagagtttgt ccttattatt
     5281 ggtctttgta tagagttctg caaggaggat gtaaatttta cttaaatcag attcgatact
     5341 tatggctttt tcaagtacca gaatggcttc gtcaaaccgg ttggctttat ataaagcatt
     5401 tccccatttg aataaggttt cctgtaaacg atgaaagttt tgctcgtatt cgattaaagt
     5461 gtttaaattt gcaggtccgt actggagctt taaatctgta ttatttatac cgtctaaatt
     5521 gagcattggc ttattgaaat aatcaaaagc ttcctgttga tattgatagg cttgtttttc
     5581 atacgtagag tttatatcat cttctttaag cttaggaagt ccgtcaggag atatttttat
     5641 gtaatttaaa tcatcaagag gctttcttct tacaaacatg gcttttcttt ctttttctaa
     5701 aaaatcttca ttttctttct ggattttttt atcggcttta cggagcctga tgttataaaa
     5761 aataataaat aaaataaaca gactaaaaaa aacaggtaat ttcataagcc tcttcctttt
     5821 tattatgatg tttttagtta ttatattctt ttttaatgaa aatgaaaagt agttttgatt
     5881 attaatcttc tgattaactc aataaaagta aaaaattaca ttttatttaa taacttttaa
     5941 aaaagtatta attataatta aattaat
//
LOCUS       DAOSUD010000105         5742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6414_length_5742_cov_23.285212, whole genome shotgun sequence.
ACCESSION   DAOSUD010000105 DAOSUD010000000
VERSION     DAOSUD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_5742_cov_23.285212"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..781
                     /locus_tag="PLG49_10010"
     CDS             242..781
                     /locus_tag="PLG49_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ17678.1"
                     /translation="MNIGEKIKLLRMANGLTQEELANRCELTKGFISQVERDLTSPSI
                     ASLVDILECLGTNLREFFNETEDEKIVFQKDDIFIQEDTENKCEIHWIVPNAQKNNME
                     PILMILDEGGQSMIHDPHEGEEFGFVLSGTIVIHIGNKKYKAKKGDSFYFKPSRTHFI
                     SNGGKTKAKVLWVCTPPSF"
     gene            806..1849
                     /locus_tag="PLG49_10015"
     CDS             806..1849
                     /locus_tag="PLG49_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17679.1"
                     /translation="MAEHIIELVDISKTFDSTEVLKTINLYIRKNEFLTLLGPSGCGK
                     TTTLRIIGGFEQPTTGKVLFEGKDITSLPPYKRRVNTVFQRYALFSHLSVEENIAFGL
                     KIKKLDKATIKEKVRNALKLVNLEGYENRSVDSLSGGQQQRIAIARALVNEPDVLLLD
                     EPLGALDLKLRQDMQVELKRMQKQLGITFIYVTHDQEEALTMSDTIVVMKDGLIQQIG
                     TPEDIYNEPKNAFVADFIGESNIIPGIMKKDCLVEFCNTIFDCVDKGFGVNEPVDVVI
                     RPEDVEIYPDDSGKLQGIVESVTFKGVHYEMIIRTDSFAFTAHNTLMFPQNSKVSISV
                     APDNIHIMKKAVN"
     gene            1849..2673
                     /locus_tag="PLG49_10020"
     CDS             1849..2673
                     /locus_tag="PLG49_10020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17680.1"
                     /translation="MKRRFLAYPYLLWMLIFIVVPLLLVLFYSFTTATEDGFAFTLDN
                     FKTFMQPIYLKVLWRSLALAAISTIVCLILGYPAALILSSRQMSRKSFLLFLFVLPMW
                     MNFLLRTYAWLTLLEKNGFINTILSVFGIEPIQLLYNDGAVVLGMVYNFLPFMVLPIY
                     SVLKKIDPSVIEAAEDLGANQVTVFRKVIFPLSLPGVFSGISMVFMPAVTTFVISRLL
                     GGGQYMLIGNLIEKQFTAVYDWHFGSAVSIVMMVIILISMGILSKYDNDREGGGLW"
     gene            2667..3455
                     /locus_tag="PLG49_10025"
     CDS             2667..3455
                     /locus_tag="PLG49_10025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17681.1"
                     /translation="MVKNAINRIYLFIIFLFLYAPIMVLIVFSFNNSKSRSHWNGFTL
                     RWYIELFQDPVIKKALFYTIAIALLSSLIATIVGTIAAIGIHSFNDWKKTLILNINYI
                     PILNPDIVTGVSLMILFIFARIKLGFWTMLLAHITFNIPYVILSVLPKLKQLNSHLYE
                     AALDLGATPLYAFFKVILPEIRPGIVTGALLAFTLSVDDFVISFFNTSSGVTNLSITI
                     FSMARTGINPKINALSTIMFLTILILLFIVNIRTSKEVKEEKAK"
     gene            3452..4522
                     /locus_tag="PLG49_10030"
     CDS             3452..4522
                     /locus_tag="PLG49_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ17682.1"
                     /translation="MKKKKFLIVLSALLIMVAMVTACGNGSQGASDKNSNKQVLNVYN
                     WGDFIAEGVIEAFEEKYGIKVNYDTFATNEDMYVKIKSGGSHYDVVIPSDYMIERMIR
                     EGMLQKIDFNNVPNFELIDDQFKYLDYDPNNEYSVPYMWGTVGILYNTTMVDEPVDSW
                     DILWNEKYANQILMLDSLRDSIAVALIKSGYSINTRDLNELEAAKQALIEQKPLVLAY
                     VGDEVKDKMIGGEAALALVWSGEAMSAIKENPDLAYAIPKEGSNLWVDAMAIPANAQN
                     KEAAELFINFMCETDIAYKNADYIGYSTAHKEAVKLLPEEILNNLAGYPPIEDLENCV
                     VFKDLGEFTKEYDRIWTEIMAQ"
     gene            complement(4547..5539)
                     /locus_tag="PLG49_10035"
     CDS             complement(4547..5539)
                     /locus_tag="PLG49_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOQ17683.1"
                     /translation="MKKVAVIFTGGTISMKLDENIQAAIPALSPEELLTMLMGIKKMA
                     EIEAIHFGSFPGPHITPQKMLELSKLTAKIIKRPDITGVVVTHGTDNMEESAYFLDLI
                     LDEKKPVVFTGAMRNSSELGYDGPSNLAAAIATVCARESMDKGVLVVMNNQVYAADEV
                     TKTHTLALDTFQSMDFGPLGIVDQDKVIYYRERKNKKHIRTDKIEASVGLIKVASGMD
                     SSLIKSLIDLGMKGIVLEGMGRGNIPPDMVEGVKYAISKNIPVVLVSRCPKGRVLDTY
                     GYPGGGKELRNLGVILGDNLSGQKARIKLMLILGLTKDMDEIKSLFEEGLYTTK"
BASE COUNT         1802 a         1015 c         1003 g         1922 t
ORIGIN      
        1 tcattaagat aattatatat tatagtaaaa attttgtcaa tccatcagtc ttttaatgtt
       61 attttattga aaaaataaat tgaaatgtaa tttatatttt tcccagataa ttttttaatt
      121 tttttattaa aaagcattga ctttatgcga aatatatgta tataataaac cgtttcattt
      181 gtttagcatc tctaaacaaa aagtttagct attcaaaacc aaaaagaaac agggtgtatt
      241 tatgaatatt ggtgaaaaaa taaaattact gagaatggca aatggattaa ctcaagagga
      301 attagccaac cgttgtgaac tgacaaaagg ctttatttct caagttgaaa gagatttaac
      361 ttctccttct attgcctctt tagtggatat tttagagtgt ttaggaacaa atttaagaga
      421 attttttaat gagacagaag atgaaaaaat cgtatttcaa aaagacgata tttttatcca
      481 ggaagatact gaaaataaat gtgaaatcca ttggattgtt cctaatgcac aaaaaaataa
      541 catggaacct attttaatga tattggatga aggaggccaa tccatgattc atgaccctca
      601 tgaaggggaa gaattcggat ttgtgctgtc aggaaccatt gtcattcata ttggaaataa
      661 aaagtacaag gcaaaaaagg gtgacagttt ctattttaag ccttccagaa cccattttat
      721 atctaatggg ggaaaaacga aggcaaaagt actttgggtt tgcacccctc ccagtttcta
      781 aatatctgat aaaggaggaa ccgctgtggc tgaacatatc attgaattag tggatatctc
      841 aaaaactttt gattctaccg aagttcttaa aaccattaat ctttatatca gaaaaaacga
      901 atttctgacc ctgcttggtc ccagtggctg cggtaaaacc accacattaa gaattattgg
      961 cggttttgag caaccaacaa ccggtaaggt tctttttgaa ggaaaagata ttacttctct
     1021 tcctccttac aaaagaagag ttaacaccgt atttcaaaga tatgccttat tttctcattt
     1081 gagtgtggaa gaaaatattg cttttggttt aaaaataaaa aaattagata aagcaaccat
     1141 taaagaaaaa gttcgcaatg ctttaaagct cgttaacctg gaaggatatg aaaaccgatc
     1201 tgtggattct ttaagtggag gacaacagca aagaattgcc atagcaaggg ctctggtgaa
     1261 tgaacctgat gtacttttgc ttgatgaacc tttaggtgct ttagatttaa aattaaggca
     1321 agacatgcag gtagaactga aaagaatgca aaagcaactt ggaattacct ttatttatgt
     1381 aacccatgac caggaagaag ccttaaccat gtccgatacc attgtagtca tgaaagacgg
     1441 acttattcag caaataggaa cccccgaaga tatttacaac gagcctaaaa atgcctttgt
     1501 ggcagatttt attggtgaaa gtaatattat tcctggcatt atgaaaaaag attgcctggt
     1561 agaattttgt aatacgattt ttgactgtgt tgataagggc tttggagtca atgaaccagt
     1621 cgatgtcgtt ataagacctg aagacgtgga aatctatccg gatgattccg gtaaacttca
     1681 aggtatcgtt gaatctgtaa cttttaaagg ggtacattat gaaatgatta ttcgtacgga
     1741 ttcatttgca tttacagccc acaatacact gatgttccca cagaattcca aggtttctat
     1801 cagtgtagca ccagataaca tccacatcat gaaaaaggcg gtgaactaat gaaaaggcgc
     1861 tttctggctt atccttatct gctttggatg cttatcttta tcgttgtacc gcttcttctt
     1921 gtgctgtttt atagttttac gacggctaca gaagatggtt ttgcctttac attagataat
     1981 tttaagactt ttatgcagcc tatttatctt aaagttttat ggcgttctct ggctttagcg
     2041 gctatcagca cgattgtatg tcttatttta ggctatcctg ccgccctgat tttgtccagc
     2101 aggcaaatga gcaggaaaag tttcttattg ttcttatttg ttctgcccat gtggatgaat
     2161 tttttgcttc ggacttatgc ctggcttact ttgttagaaa aaaacggatt tatcaatacg
     2221 attttatctg tttttggcat agaacccata caactgctat ataatgatgg tgctgttgtt
     2281 ctgggtatgg tttataactt cctgccattt atggttttac ccatttactc ggtactgaaa
     2341 aaaatcgatc ccagtgtcat cgaagctgcc gaagatttag gagcgaatca agtgacggtt
     2401 tttagaaaag tcatattccc tctaagtctt ccaggtgtct tttcaggcat ttccatggta
     2461 tttatgcctg ccgtaaccac ctttgttatt tcaagactgc ttggcggcgg tcaatacatg
     2521 ttaattggta acctgattga aaaacaattt actgctgtat acgactggca ctttggttct
     2581 gctgtatcga ttgtcatgat ggtcatcatt ttaattagta tgggcatttt atcaaaatat
     2641 gataatgacc gtgaaggagg gggattatgg taaaaaatgc aatcaaccgg atttacttgt
     2701 ttattatctt tttgtttctc tatgcaccta ttatggtcct tattgttttt tcctttaata
     2761 attccaaatc ccgctcccat tggaatggtt ttaccttaag atggtatatt gaattgtttc
     2821 aggaccctgt cattaaaaaa gcattgtttt atacaatcgc aattgcactt ttgtcttctc
     2881 ttatcgcaac cattgtaggg actattgctg ccataggtat acacagtttc aatgactgga
     2941 aaaaaacttt aatattaaat ataaactata ttccaatact caatccggac atcgtcacgg
     3001 gtgtatctct tatgatttta tttatctttg caagaattaa attaggcttt tggactatgc
     3061 ttttggcaca cattaccttt aatattcctt atgttatatt atcggtattg ccaaaactaa
     3121 agcagttaaa cagccattta tatgaggctg ccctggacct tggggcaaca cccttatatg
     3181 ccttttttaa agtcattctt ccggaaatca ggccaggtat tgtaactggt gctttattag
     3241 cttttacttt gtctgttgat gactttgtta ttagcttttt taacaccagt tctggtgtca
     3301 ccaatttatc catcaccatt ttttcaatgg caagaaccgg tattaatcca aaaattaatg
     3361 ccctgtctac cattatgttt ttaaccatat taattttatt atttattgtt aatataagaa
     3421 catcaaaaga agtgaaggag gaaaaagcaa aatgaaaaaa aagaaatttc ttattgtctt
     3481 gtcagcttta ttaatcatgg ttgctatggt tacggcttgt gggaatggtt cacaaggcgc
     3541 ttctgacaag aattccaata aacaagtttt aaatgtttat aactggggag attttatcgc
     3601 tgaaggagtt attgaagcat ttgaagaaaa gtatggcatt aaagtaaatt acgatacctt
     3661 tgccaccaat gaagatatgt atgttaagat taagtctggt ggaagtcatt atgatgttgt
     3721 cattccttcc gactatatga ttgaaagaat gattcgggaa ggcatgcttc aaaaaatcga
     3781 ttttaataat gtccccaatt ttgaacttat cgatgatcag tttaaatacc ttgattatga
     3841 cccaaataac gaatattccg taccttatat gtggggaact gtcggtattc tttataatac
     3901 aaccatggta gatgaacctg tagacagctg ggatatcctt tggaacgaaa agtatgccaa
     3961 tcaaatcctt atgttagaca gtctgagaga ttctattgct gttgccctta ttaaatcagg
     4021 ctattccatc aataccagag acttgaatga attagaagca gcaaaacaag ctttaattga
     4081 acaaaaacct ctggttttag cctatgtagg cgatgaagta aaagacaaaa tgattggcgg
     4141 ggaagctgcc cttgctcttg tttggtctgg agaagccatg tctgctatta aagaaaatcc
     4201 ggatttagct tatgccattc ctaaagaagg ttcaaatctc tgggttgacg ccatggcaat
     4261 accagcaaac gcacaaaata aagaagctgc tgaattattt attaatttta tgtgcgaaac
     4321 agacattgct tacaaaaatg ctgactatat tggttattct acagcacata aagaagctgt
     4381 aaaattatta cctgaagaaa tattaaacaa cttagccggt tatcctccta ttgaagattt
     4441 agaaaattgt gttgtattta aagatctagg ggaatttaca aaagaatacg acagaatttg
     4501 gacagaaatt atggctcaat aaaaggatgg tcaatgacca tcctttttat tttgtcgtat
     4561 ataaaccttc ttcaaagagg ctttttattt catccatgtc cttagtcaat cctaaaatca
     4621 gcataagttt tatcctggcc ttttgccctg acagattatc ccccaaaatg acacccagat
     4681 ttcttaactc ttttcctccg cccggataac catatgtatc taataccctc cctttaggac
     4741 atctggatac caatacaacc ggtatatttt tagaaatggc gtactttacc ccctccacca
     4801 tatccggagg aatattgccc cttcccattc cttcaagaac aattcctttc atccctaaat
     4861 caataagact tttaattaaa gaagaatcca tccctgaagc tacttttata agaccaacac
     4921 tggcttctat tttatccgtt ctgatgtgtt ttttattttt tctctcccga taataaatca
     4981 ctttatcctg gtctacgatt cctaaaggtc caaaatccat gctctgaaag gtatctaaag
     5041 ccagcgtatg ggtttttgtc acttcatctg ctgcataaac ctgattgttc atcaccacca
     5101 gtaccccttt atccatagac tccctggcac atacggtggc aatggcagct gctaaattag
     5161 aaggaccgtc atagcctagt tcagaactgt ttctcatggc tccagtaaaa acaacaggct
     5221 ttttttcatc taaaattaaa tctaaaaaat aagcactttc ctccatatta tctgtcccat
     5281 gagttaccac tactccagtg atatccggac gttttataat cttagctgta agctttgaca
     5341 attccaacat cttttgcggc gtaatatgag gcccaggaaa acttccaaaa tgaatggctt
     5401 ctatttcagc catctttttt atacccatta acatggttaa tagctcttca ggagataagg
     5461 caggtatagc agcctgtatg ttttcatcca gcttcataga aatggttcca cctgtaaaga
     5521 tcactgcaac ctttttcaaa gaaatgcctc cttttgtgca ttaaaagata tgttccattt
     5581 tatcagactt ttataaaatt agaattgaat catagcattt attttattca agaagttgat
     5641 tttatttaat tgttgcagat aactttttaa aacacagttc gctattttta cttttcattt
     5701 ttgttatgct ataataaaat tataaaaaat gactaaatga ta
//
LOCUS       DAOSUD010000106         5707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6449_length_5707_cov_26.291401, whole genome shotgun sequence.
ACCESSION   DAOSUD010000106 DAOSUD010000000
VERSION     DAOSUD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_5707_cov_26.291401"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1188
                     /locus_tag="PLG49_10040"
     CDS             211..1188
                     /locus_tag="PLG49_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOQ17684.1"
                     /translation="MSGTYIFTLFYKSLNYSFPVGLIINMVTIIICMIIRNTNVLEKI
                     RNRVNKFNNIFVIVLFNIVFVAVAFLYVWENNKDIVWNYSFVWLFAVACWTIINFYIL
                     FQTIKIKEQEEKLYIHERYVPFLTELVEEARRKQHDYANHLNAIYGLTELDDSNLCKK
                     EIRKYLKGLIRDFKTLDKILAIREPVLSAIIYSKRALAESQNISFKLEIINQIPHYPV
                     EDYELVEILGNLLDNAIEAINLNNTEFAKEVILTLGEEEEKKVIEVKNSGEPFELKNI
                     ETLFEKGFSTKKGKHRGYGLYNVKKVVNKYNGTIELSLADNYTIFRVLF"
     gene            complement(1180..1320)
                     /locus_tag="PLG49_10045"
     CDS             complement(1180..1320)
                     /locus_tag="PLG49_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOQ17685.1"
                     /translation="MKKTLLSSQSILFSLASFFVIAAGILTKRCSIFMWGEPKCPKSL
                     LK"
     gene            complement(1317..1886)
                     /locus_tag="PLG49_10050"
     CDS             complement(1317..1886)
                     /locus_tag="PLG49_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOQ17686.1"
                     /translation="MSLKYLINLFAYKIYKEKILPHSDIRKMIYGMTVLSNELIKIII
                     LIIFFKLIGHLPLFLFSFCILMSIRTYSGGLHFENSIACFFFSLVFFLLSVVILPKFF
                     LLNFYHGLIIGLSGVLILSLNSPRPSALRPIINKKRRKTFKLLSALSSTICLVILLTL
                     IKSPIFFTCGIWTIFLQAMQLFLGKEEKE"
     gene            2306..3097
                     /locus_tag="PLG49_10055"
     CDS             2306..3097
                     /locus_tag="PLG49_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ17687.1"
                     /translation="MIESIQNKHIKWLIALQKNRKNREKEKLFIAEGIKIIEEIPADW
                     EIINLFVSSSFIREHHSFIKDIKIDKKNIIEVADKVFNAISDTVTPQGILAVCRQKIF
                     SLEDILSVKNGFFIILEEIQDPGNLGTIIRTADAAGADAIFLTKGSVDLYNPKVIRST
                     MGSIFHLPIFTEMDIDTLIEELKNSNVSILSAHLKGNLYPYQYNLKKASALLIGNESK
                     GIKESTAQKTDALLKIPMLGKAESLNAAVAASILMYEVVRQRIEI"
     gene            3128..4213
                     /locus_tag="PLG49_10060"
     CDS             3128..4213
                     /locus_tag="PLG49_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17688.1"
                     /translation="MEKIMRSIVTFILIVTLSLGFFNLSWADEILKDEIPQSMKTAIT
                     EVLEDLATALGKVDYASYLELFHKKSPIYTMHYSELKETLNILSSYELNYEVESIKFI
                     EHNNKEIKVEVDILVQSHKNDEYYDRKNTFELIFREDNGKIKIYDMIIRNIEFLDEKD
                     KINSVLGKKRLYYNDGSLAYEGYVKNGLPDGEGIRYYNNGNIMYEGTFKEGEMTGKGI
                     LYDEQGNKVYEGEVKNGLPDGEGTAYYYSGKVYYKGYWKNGLFNRRGKLHYENGLTMY
                     IGEFSEGKAEGKGKGFYENGILCYEGEMKNNYSHGFGIEYYETGEIMYEGEFAYGLPH
                     GKGKKYDKEGNIIFSGYWENGSMVTDK"
     gene            4599..5048
                     /locus_tag="PLG49_10065"
     CDS             4599..5048
                     /locus_tag="PLG49_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOQ17689.1"
                     /translation="MSFWQGFSSKVKNIAQNVAKKSGDMVEVTKINLSISTEEENIKK
                     LYTEIGKYCYEIYEKNVRFDNTINELCEKIKAHKNAIESLNEKINELKNVVKCQACGN
                     VLPRDSVYCAKCGVKIEIKEAQIEAETEKNSMIIEANQEVQNRNSSL"
     gene            5135..>5707
                     /locus_tag="PLG49_10070"
     CDS             5135..>5707
                     /locus_tag="PLG49_10070"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOQ17690.1"
                     /translation="MEKEVLPQKIKFNLNTDFLKLIAAVTMFIDHIGAVLFPDILMFR
                     IIGRISFPLFAYCLVVGFLYTSNIKKYALRLLIFGIISQPIHVFTFDVSWAQLNILPT
                     LLMGLILLYCLREKKWFIFILLIVSVFFINFNYGIEGILLIILFYVFRNHTFAALIST
                     SLLLIKPLFYGHIQGFAVFCIPFIFFHTNVK"
BASE COUNT         2102 a          637 c         1031 g         1937 t
ORIGIN      
        1 attgggtcaa tttttaaccg gcgattcggt caaaaatagc ccggcgttga catcttggca
       61 ttattatccg taataaccat taatcataat ataaatttta tttttatcta tctggctatc
      121 ttagtggtta ctaaaataat gtttaaggtt ccgtttagct ctattttctt ttcttttatc
      181 atatctttta tgattattat tggggttcaa atgtcaggaa cgtacatttt taccttattt
      241 tataaatcgc tgaattatag ttttccagtt ggtttaatca ttaatatggt aacgattata
      301 atttgtatga ttattagaaa tacgaatgtt ttagaaaaaa ttagaaacag agttaacaaa
      361 tttaataata tttttgtgat tgttttattt aatattgttt ttgttgcggt tgctttttta
      421 tatgtctggg agaataataa agatattgtt tggaattata gttttgtatg gttatttgca
      481 gttgcatgtt ggacaattat taatttctat attttatttc aaaccataaa aataaaagag
      541 caagaagaaa aattatatat acatgaaaga tatgtccctt ttttaaccga attagtagaa
      601 gaagcaagaa gaaagcagca cgattatgcc aatcatctta atgctattta tggtctgact
      661 gaattggatg attctaattt atgtaaaaaa gaaataagaa aatatcttaa aggattaatt
      721 agagatttta agacattaga taaaatattg gctataagag aaccagtttt aagtgctatt
      781 atatatagta agagagcttt agcagaatct caaaatatca gttttaaatt ggaaattatt
      841 aaccaaattc ctcattaccc agtagaggat tacgaattgg tggaaatatt gggaaattta
      901 ctagataatg ccatagaagc cattaattta aataacacag aatttgcaaa ggaggttatt
      961 ttgactttag gagaggagga ggaaaagaaa gttatcgaag taaaaaattc aggagaacct
     1021 tttgagttga agaacataga aactcttttt gaaaaaggtt tttcaacaaa aaaaggaaag
     1081 catcgtggtt atggccttta taatgtaaaa aaagtcgtta acaaatataa tggcaccatc
     1141 gagctttcct tagcagataa ttatacaatt tttagagtct tattttaata agctttttgg
     1201 gcatttaggt tcgccccaca tgaagataga acagcgttta gttaagattc ctgcagcgat
     1261 tacgaagaaa gatgcgagtg agaataaaat agattgagaa gaaagcaggg tttttttcat
     1321 tctttttcct cctttcctaa aaataattgc attgcctgca ggaatatggt ccatattccg
     1381 caggtgaaga atattgggct tttgatgaga gtcagtagaa ttactagaca aattgtagat
     1441 gaaagggcgg atagtaattt gaaggttttc cttctttttt tattaataat agggcgtaaa
     1501 gctgatggcc gtggggaatt tagggagagg atgaggacac cgcttagccc tattattaat
     1561 ccatgataaa agtttagaag aaagaattta ggtaaaataa caacggataa taaaaagaaa
     1621 acaagcgaga agaaaaaaca agcgatagag ttttcaaagt gcaatcctcc agaataggtt
     1681 cttatggaca ttaaaataca gaatgaaaat aaaaataaag gaagatgtcc aattagttta
     1741 aagaaaatga tgaggattat aatttttatg agttcattgg ataggacggt cataccataa
     1801 atcatttttc gtatgtcact gtggggtaat attttttcct tataaatttt ataagcaaat
     1861 aaattaataa gatatttcag tgacatttat catcccgtct ttcgtttttt ctatggaaat
     1921 tgtagcacat aacgttatat ttgtgcgtaa aatgtaatga aatggtagtt aaatgtaata
     1981 aaagataaaa atatgttatt tcgttacatt tcactaccag ttcgtaacat ttaagtggca
     2041 atatttaggt tttcaagaga gatatatcat gacagctgtc atggtatttt tttattttct
     2101 tttaaagatg aataaaaaac agtgaaaaat gaaatattct atcaaattct gtatttttga
     2161 cttttttatt cattgtgtta taatatttaa gatttaataa gactttggtc ccatatggaa
     2221 tagcataaaa aagaatgatt ttatgatgat ttggtcataa tgaaaaagaa aataatgata
     2281 tggagaaatt gaaagaagtg tttttatgat tgaaagcatt cagaacaaac atataaagtg
     2341 gcttatcgct ctgcaaaaaa acagaaagaa ccgtgaaaaa gaaaaattgt ttatagcaga
     2401 agggataaag attattgaag aaattccggc agattgggaa atcattaatc tttttgtttc
     2461 cagttcgttt attagggagc atcattcctt tataaaagat ataaaaattg ataaaaaaaa
     2521 tatcatagaa gtagcagata aagtatttaa tgcaatttcc gatacggtta caccgcaagg
     2581 catattagct gtatgcagac aaaaaatttt ttctttagaa gatattttat ctgttaaaaa
     2641 tggatttttt attatccttg aagaaataca agatccgggt aatcttggga ccattataag
     2701 gactgcagat gcagcaggag cagatgcaat ttttttaaca aaaggcagtg tggatttata
     2761 caatccaaag gtgatacgct caacaatggg ttcaattttc catctgccta tttttacaga
     2821 aatggatatt gacacgctta tagaagaatt gaaaaatagt aacgtttcaa ttttgtcagc
     2881 tcatttaaag ggaaatttat acccttatca atacaattta aaaaaggcca gtgctctttt
     2941 aatcggaaat gagtcaaaag ggataaaaga gtctactgct caaaaaacag acgctttatt
     3001 aaaaataccg atgctgggaa aagctgaatc attaaatgcc gcagtagcag cttctatcct
     3061 tatgtatgaa gttgtaaggc agagaattga aatataaaat gattacaaga gaaaaggaga
     3121 aataatcatg gaaaagatca tgagaagtat tgtcactttt atattgatag taacgctcag
     3181 tctgggattt tttaatttat cctgggcaga tgaaatttta aaagatgaaa ttcctcagtc
     3241 catgaagaca gcaataacag aggtattgga agatttagcg acagctttag ggaaagtaga
     3301 ttatgcttct tatttagaat tgtttcataa gaaaagtccc atttatacga tgcattattc
     3361 tgaattaaaa gagacgctta atattttatc ctcttatgag ttaaattatg aagttgaaag
     3421 tattaaattt attgagcata ataataaaga gataaaggtg gaagttgata tattggttca
     3481 atctcataaa aatgatgaat attacgaccg taaaaatact tttgaactca tttttaggga
     3541 agataatgga aaaataaaaa tatatgatat gattataaga aatattgaat ttcttgatga
     3601 aaaagataaa attaacagcg ttttaggcaa aaaaagactt tattataatg atgggagtct
     3661 tgcatatgaa ggttatgtaa aaaatggtct tcctgatgga gaaggaatca gatattataa
     3721 taatggtaat atcatgtatg aaggaacttt taaagaagga gaaatgacag gaaaagggat
     3781 actttatgat gaacagggaa ataaggttta tgagggagaa gtaaagaatg gtcttcccga
     3841 tggtgaagga acagcatatt attatagtgg aaaagtttat tataaaggat attggaaaaa
     3901 tggcttattt aacagaagag gtaaattgca ttatgaaaac ggcttaacaa tgtatattgg
     3961 agaattttcg gagggtaaag cagaaggtaa aggtaaaggc ttttatgaaa atggtattct
     4021 ttgttatgag ggtgagatga aaaataatta ttctcatgga ttcggaattg aatattatga
     4081 aacaggagaa atcatgtatg aaggagaatt tgcctatgga cttcctcatg gtaagggcaa
     4141 aaaatatgat aaagaaggca atatcatttt ttcaggatat tgggaaaatg gatcgatggt
     4201 aaccgataaa taaaaaaggt tgtgcattaa aggttcagca ccgttagtgc acagcttttt
     4261 tattttcgaa ggtttttatg attttcatgc ttcgaagctt tcagcttcat atcaatttag
     4321 aatttgtctt agtttataat tagttaaatt aaattcttgt cagctatttt ttataaaatt
     4381 ctttttgaag attaagaaca gaaaaaatta atatttattg tattaaaata tttaaattat
     4441 taagcaaata ttaaatagtg gaaattaatt ataaatacat gtataataat taataacttt
     4501 tacttttatc tattattttt gataagctgt ttgatggatt tttatatatc cggggatgtt
     4561 tttaaatccc gtaaaatttt aaagaggaga tgagcaatat gtcattttgg caaggttttt
     4621 caagtaaggt taaaaatatc gctcaaaatg tagcaaaaaa atcaggtgac atggttgaag
     4681 tgacaaagat aaatttgagt atcagtacgg aagaagaaaa tattaaaaag ctttatacag
     4741 aaatcggaaa atattgttac gaaatatatg aaaagaatgt gagattcgac aacacaatca
     4801 atgaactatg tgaaaagata aaggcacata agaatgcaat tgaaagtctt aatgaaaaga
     4861 taaatgaatt aaaaaatgtt gtaaaatgcc aggcgtgtgg taatgtactt ccaagagaca
     4921 gtgtttattg tgctaaatgt ggggtaaaaa tagaaataaa ggaagctcaa atagaggcag
     4981 aaacagagaa gaatagtatg atcattgaag caaatcagga agttcaaaat agaaactcat
     5041 cgttataatt aataaatttg tgttataata agggggtatt attatatcat gatatccctt
     5101 tttttattga ttgaatactg gtacaggaga acttatggaa aaagaagtat taccccagaa
     5161 aattaaattt aatttaaata cagatttttt aaaattaata gctgctgtca ccatgttcat
     5221 tgatcatatc ggtgctgtct tatttcctga tatccttatg ttcagaataa taggcagaat
     5281 ttctttccct ttatttgctt attgtctggt ggttggtttt ctttatacaa gcaatataaa
     5341 aaagtatgct ttaagattat tgatattcgg aataatatct cagcccatac atgtttttac
     5401 ctttgatgtt tcctgggctc aattaaatat cctgccaact ctattaatgg gcttaattct
     5461 tctttattgt ctcagggaaa agaaatggtt tatatttatt ttactcattg tatccgtttt
     5521 ttttattaat tttaattatg gcatagaagg cattcttctt atcatcttat tttatgtctt
     5581 cagaaatcat acttttgctg cactaatatc aaccagtctg ttgttaatca aaccactttt
     5641 ttatggtcat atacaaggtt ttgctgtttt ttgcattcct ttcatttttt ttcatacaaa
     5701 tgttaaa
//
LOCUS       DAOSUD010000107         5601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6566_length_5601_cov_23.074829, whole genome shotgun sequence.
ACCESSION   DAOSUD010000107 DAOSUD010000000
VERSION     DAOSUD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_5601_cov_23.074829"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..237
                     /locus_tag="PLG49_10075"
     CDS             73..237
                     /locus_tag="PLG49_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17691.1"
                     /translation="MSKKTNKTNNNNKNNNNVNVDFANELTPNKTNKTNNNNNNNNNN
                     NNNNNRNNCK"
     gene            407..2921
                     /locus_tag="PLG49_10080"
                     /pseudo
     CDS             407..2921
                     /locus_tag="PLG49_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(2989..4524)
                     /gene="spoVB"
                     /locus_tag="PLG49_10085"
     CDS             complement(2989..4524)
                     /gene="spoVB"
                     /locus_tag="PLG49_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HOQ17692.1"
                     /translation="MNKKNIITGALILTSANFITRILGFVYRIYMSNLIGAEGMGLYQ
                     LIFPIYLLAWTISSSGISLSVSKYVAQENAKKEYGNSARILKASVILSAGTGLMISFF
                     IFFFASFLANSVIKEARTYLSLKILAFCIPFMSAASAIKGYFYGLQEMFKPAAAQVIE
                     QISRMAVIALLASLFIHKGLSYACALAVLGICAGEIISFIYIFFAYRYDKNRKRHVKK
                     ASISMLKAYYLLLSMAIPLTVSKFITSFLSSIENIMIPQKLQHFGMTANEAIGIYGQF
                     SGMAMPLIFFPSILIGALSMTLVPAVSEAKAVRNTRQIHLTTSKSIHFTSLIGIGATC
                     IFLVFSKELGIAFYNQENVGDMLYSMAWICPFFYLQATLSGILNGLGQQMILFRNNII
                     GSFISIGFIYFLIPKLGLLGFIWAVIASSITVTLLHLGNVLKFTSISLDLNKWIIRPA
                     LAAAATGLTSKYILNHYLLSRFSLKSSLFIVLVILALMYVFFLFLVQSITMEDIKLLR
                     KNH"
     gene            4700..5509
                     /locus_tag="PLG49_10090"
     CDS             4700..5509
                     /locus_tag="PLG49_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOQ17693.1"
                     /translation="MDLNEWKRFLIPYEQAVEELKVKFMGIREEYIRLEEYSPIEYVT
                     GRVKKISSILEKAQRKGIPLEEIEKKIEDIAGVRIICQFVKDIDKVVKLIRERDGKDL
                     RIVEERDYIKNIKPSGYRSYHIIIKYPLQTIRGEKEILTEIQIRTLAMNFWATIEHSL
                     RYKYKKDMPDYIHKRLLSAAEAAFTLDKEMSEIREEVLKAQEQFEARSNMIAEIFTNI
                     QNLSKINEIKEAGKLYQKFWEIWEEGDIEKLEDFNRKLDILAEIYGAQRLH"
BASE COUNT         1993 a          810 c         1161 g         1637 t
ORIGIN      
        1 aaataattct gttttttttt gcttatacta atgctgtaat cataattaaa taatataagg
       61 aggctttctt taatgagcaa aaagactaat aaaaccaata ataataacaa gaacaataat
      121 aatgtaaatg tagattttgc aaatgaatta acccctaata agactaataa aacaaataac
      181 aacaataata acaacaataa taacaacaat aacaataacc gtaataattg caaataagat
      241 aaaaaatccc cgtgatcatc atggggattt tttctgattt tgaataaata aataatatta
      301 ataattacta tcaattaaat attgacctta gattaaaata attgtataat aaactcaacc
      361 cctcgtaggg aggggtataa aaattgagcg gggaatgata gatattatgg aaaaaaaaca
      421 atttgatata tctggtatga cttgcgcagc ttgtgccagg gctgtggaaa aagtgtaagt
      481 aagctggacg gcatagtaga agccaacgtc aattttgctt ctgaaaaatt aaatgtccag
      541 tatgatgaaa ataaacttaa aatagagcaa attattcaag ctgttgaaca tgcaggttat
      601 aaagctgagg aatctgttga aaataagaaa aaagacgata aagataagga aataataagt
      661 ttaagaaata agctgatttt tgctgcagtt tttacaattc ctttattgta tatttctatg
      721 gggcatatga taggtctacc tttacctgga tttttaacag ggcatgaaaa cgctttaaat
      781 tttgctttga ttcaattcat cctgaccata cctgtggtca ttgcgggtta taaattttat
      841 actgtaggat tcaggacttt gtttaaagct tctcccaaca tggattctct tattgccctg
      901 ggaactggag cagccattct ttatggtctc tttgccattt ataaaattat gacaggtact
      961 catgaagaag tgattgctta ttccatggat ttatattttg aatcggcagc cgtgattatt
     1021 actttaattc ttttgggcag atattttgaa gccttagcca aaggcaggac ttcagaagcc
     1081 attaaaaaac tgatgggtct tgccccaaaa acggctgtga ttataaaaga aggcaaagaa
     1141 gtagaaatcg ctattgaaga agtaaaggtc ggagatatta ttgtcgtaaa acctgggcaa
     1201 aaaatacccg tagacggaga tgttattaaa ggaacaacgg cagtggatga atctatgtta
     1261 acaggtgaaa gtattccggt agagaaaaaa acaggggatc cggtcgtggg tgctacaatt
     1321 aataaaacgg gttcaataca atttaaagca acaaaagttg gtaaagatac tgtgttagcc
     1381 cagattgtaa gactggtgga agaagcccag gggtcaaaag cgcctattgc aaaaatggtc
     1441 gatgtcatta gcagttattt tgtaccagtt gttttagtca ttgcttttgc tgcaggcgta
     1501 ctctggtatc tttccggaga atctttggtt ttttctttaa ccatgttgat ttcggtattg
     1561 gtgattgcct gtccttgtgc cctgggttta gcaacaccga ctgctattat ggtgggtact
     1621 ggaaaaggag ctgaatatgg tgttttaatt aaaagtggta cagctctgga aacggctcat
     1681 aaagtcaata cgattgtttt tgataaaaca ggaaccctta cagaaggaag accagaacta
     1741 acggatatta tttcttatca tggcataaca gaagatgaac tcttacagct ttctgcttcc
     1801 gctgaaaaag cttcagaaca tcctctggga gaagccattg taagaaaggc tcaggaaaaa
     1861 aactttctct ttttgaaaca gaagagttta atgcaattcc cggtcatggc attgaagtta
     1921 agataaatga gcaaaaaatt attctgggca ataaaaaact aatgctggat agacaggtgg
     1981 atatccatga agctgaaaaa acggcagacc gtctggcttt ggaaggtaaa agtcccatgt
     2041 atgttgctgc tgacaaccgt ttgctgggta ttattgccgt ggcagatgtt gttaagggaa
     2101 atagtattaa agccattaaa aagctccatg atatgggaat tgaagtggtt atgattacag
     2161 gggacaataa aaggacagcc gaagcgattg ctaagcaagt tggtataaac agggttctgg
     2221 cagaagtcct gccacaagat aaggccaaag aagttaagaa aattcaagaa gaaggtaaaa
     2281 aagtggccat ggtaggagat ggtattaatg atgccccagc cttagcgatg gcagatgtgg
     2341 gtattgccat tggttccgga acggacgtgg ctatggaatc ggctgatata gtcctgatga
     2401 aaagcgatat tttagatgtg gtaacggccc tgcagctaag taaaagtacc ataagaaata
     2461 ttaaacaaaa tctcttttgg gcctttttct acaacagtgc aggtatacct cttgcagcag
     2521 gtctttttta cctttttggc ggacccaaac taaatccaat gtttgcagca gcagccatga
     2581 gttttagttc tgtatcggtg gtattaaatg ctctgcgttt aaaagctttt aagcctgatg
     2641 ctgataaaga aaatgaaagt tataaaaaag ttttaaaaag tgaagagaaa gagggcgata
     2701 tgatgaaaaa gaagctttat attgaaggta tgagttgtca acactgtgtg aatcatgtgc
     2761 aaaaagcttt aagccaaatt tcaggtattg atgctgttaa tgtggacttg gaaaataaat
     2821 atgctatagt ggatatgaaa gaagaagttt ctgatgattt attaaaacag gctgttgttg
     2881 atgaagcagg ttatgaactt gtaaagattg aaaaactata attaaaaaag tccgcctaag
     2941 aggcggactt ttttagaatt tgaaatcttc tggttaaaca agagattttt aatgattttt
     3001 tcttagtaat tttatatctt ccattgtaat gctttgaact aaaaagagga aaaaaacata
     3061 cataagagca aggatgacca gaactataaa taagctgctt tttagggaaa aacgggataa
     3121 aagataatgg ttcaggatat attttgatgt taaacctgta gcggcagccg ccagagcagg
     3181 tcttattatc cacttgttta aatccaggct aatagaggta aattttaaga catttcctaa
     3241 atgaagcagg gtaacggtta tggaactggc aatgactgcc catataaagc ctaaaagtcc
     3301 tagtttgggt attaaaaagt agataaatcc gatactaatg aaagaaccaa tgatattgtt
     3361 tctaaataaa atcatttgct ggccaagacc atttaagatg ccggacaagg ttgcctgaag
     3421 ataaaaaaag gggcaaatcc aggccatgga ataaagcata tcaccgacgt tttcctgatt
     3481 ataaaaggca atgcccagtt cttttgaaaa aactaaaaag atacaagtag caccaattcc
     3541 aattaaagaa gtaaagtgaa tggattttga agttgtcaga tgaatttgtc tggtatttct
     3601 tacggctttt gcttcagaaa cagcgggaac taaggtcata gacagtgctc ctataagaat
     3661 agaagggaaa aaaattaaag gcatagccat accgctgaat tggccgtaaa taccaatggc
     3721 ttcattggca gtcatgccaa aatgttgaag cttttgtgga atcataatat tttcaatcga
     3781 actgagaaaa gaggtaataa atttgctgac ggttaaagga atagccatag acagtaaaag
     3841 atagtatgct ttcagcatgc tgatagaagc ttttttcaca tgtctttttc tgtttttatc
     3901 atagcgatat gcaaagaata tataaatgaa agaaatgatt tctccggcac agataccaag
     3961 gactgcaagg gcacaggcat aggaaagacc tttgtgaata aatagtgagg ccagtagagc
     4021 aatcactgcc attctgctta tttgttctat gacctgggct gctgctggct taaacatttc
     4081 ctgtaagcca taaaaatagc cttttatggc tgaagcagcg gacatgaagg gaatgcaaaa
     4141 agctaaaatt tttaaagaaa gataagttct ggcttctttt ataactgaat ttgccagaaa
     4201 ggatgcaaaa aagaagataa aaaatgaaat cataagacca gttcctgcag acagaataac
     4261 ggatgccttt aatatccggg cactgttgcc gtattctttt tttgcatttt cctgggcaac
     4321 atattttgaa acgcttaagg agataccaga actagatatg gtccaggcca aaagataaat
     4381 tggaaagatt aattgataga gccccattcc ttctgcacca attaagtttg acatatatat
     4441 acggtaaaca aagcccagaa ttcttgtaat aaaattagca gaagtcagaa tcagggctcc
     4501 tgtaataatg ttttttttat tcattcaata ccctctttcc atgggacggt attacaattt
     4561 attcgttttc aagttgtact atgacaaata atattctatt gttattgtta attgactgga
     4621 ttttctatat aattaaaatt ggagtaacag agtgtgttga aatgtttgct ttaataattg
     4681 aaataaggaa gtgagcaaca tggatctgaa tgagtggaaa aggtttttga ttccttatga
     4741 acaagcggta gaagagctta aagttaaatt tatgggtatt cgggaagaat atatcaggtt
     4801 agaggaatat tctcccattg aatatgtcac tggcagggta aaaaagatat ccagtatttt
     4861 ggaaaaagct caaagaaaag gaattcccct cgaagaaatt gaaaaaaaaa tcgaggatat
     4921 tgctggagta agaattatat gccagtttgt taaagacatt gataaagtgg taaaactcat
     4981 tcgtgaaaga gatggaaaag atttaagaat tgttgaagaa agggattata taaaaaatat
     5041 taaacccagt ggctatagaa gttatcatat tataatcaaa tatcccttac aaaccattcg
     5101 tggtgaaaaa gaaattttaa cggagattca aatccgtact ttggccatga atttttgggc
     5161 aacgattgaa cattcattaa ggtacaaata taaaaaggat atgccggatt acattcataa
     5221 gagactgctc agtgctgcag aagcggcatt tactttagat aaagaaatgt ctgaaataag
     5281 agaagaagtc ttaaaagcgc aggaacaatt tgaagccaga agtaatatga ttgctgaaat
     5341 ctttacgaat atccagaatc tatctaaaat caacgaaatt aaagaagcag ggaaattata
     5401 tcaaaagttt tgggaaattt gggaagaagg agacatagaa aaattagaag attttaatag
     5461 aaagttagat atactggcag aaatatatgg agcacaaagg ctccattaat tcgaaggttt
     5521 ttggccgaag tactggtagt aatctacttt tattcggcca ttataaagct ttcttttttt
     5581 attggctttt ttgccaaaat a
//
LOCUS       DAOSUD010000108         5584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6586_length_5584_cov_23.896907, whole genome shotgun sequence.
ACCESSION   DAOSUD010000108 DAOSUD010000000
VERSION     DAOSUD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_5584_cov_23.896907"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1157)
                     /gene="hemW"
                     /locus_tag="PLG49_10095"
     CDS             complement(18..1157)
                     /gene="hemW"
                     /locus_tag="PLG49_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOQ17694.1"
                     /translation="MDKCVGLYIHIPFCKAKCYYCDFTSFDNQNEKMVPYVFALCEEI
                     KQYGEILQDRQVDTIFIGGGTPTVLPLALLEKILKSLKENFKIESSFEFTVESNPGTL
                     NKELLYLLKEYRVNRLSMGLQATQNKILKSLGRIHSLEDFYNNYHLARQMGYQNINVD
                     LIFSLPDQTKKEWADTLKYIIKSEPEHISCYSLIIEENSYFGRLKKEGKLKEASDELD
                     RELYHYARKILTENKYHHYEISNFSKKGFESRHNMNCWKYKPYVGMGLGAHSFINGQR
                     YHNSYNLDKYIQSQGKIESIKEEIESIPLTMQYGEYIFLGLRLTEGISIKDFESQFNI
                     SFHALFGEKVKKLIEEGLLIKKQDRIQLSLKGLDLANIVFAEFLP"
     gene            complement(1163..2971)
                     /gene="lepA"
                     /locus_tag="PLG49_10100"
     CDS             complement(1163..2971)
                     /gene="lepA"
                     /locus_tag="PLG49_10100"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOQ17695.1"
                     /translation="MVLSKQERTRNFCIIAHIDHGKSTLADRIIQKTGLLTEREMQDQ
                     ILDNMDLERERGITIKSQAVRLVYKAEDGEEYIFNLIDTPGHVDFNYEVSRSLAACEG
                     AVLVVDAAQGIEAQTLANVYLAIEHNLEIVPVINKIDLPSANPENAKREIEDIIGIPA
                     EDAPLISAKMGINIEAVLEKIVKEIPSPQGDENAPLKALIFDSVYDSYKGVIAFIRIK
                     EGKIKKDMRIRMMSTGKEFEVNEVGVFGPGQFIVTNELTAGDVGYITASIKNVKDSRV
                     GDTITDADNPASEPLPGYKKVNPMVYCGMYPADGAKYEDLREALEKLQLNDAALVFEP
                     ENSVALGFGFRCGFLGLLHMEIIQERLEREYNLDLVTTAPSVIYKVHKTNDEIIELSN
                     PTDLPDPSEIKYMEEPVVKAEIIVPTEYIGSVMELCQERRGEYIGMDYLEETRAVLTY
                     HLPLNEIIYDFFDALKSRTRGYASFDYELIGYQESDLVKLDILVNREVVDALSFIVHK
                     DKAYERGRKIAEKLKQEIPRHLFEIPIQAAIGNKIIARETISALRKDVLAKCYGGDIT
                     RKKKLLEKQKEGKKRMRQIGSVEVPQSAFMSVLKLE"
     gene            complement(3133..4419)
                     /locus_tag="PLG49_10105"
     CDS             complement(3133..4419)
                     /locus_tag="PLG49_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOQ17696.1"
                     /translation="MVRIHTISLKKIKTQLWIGLLLLLVIGIIKLQEDKKLTGDIHLD
                     RQITKYLKDQTLDSRTYKKILEETIPYVEDYSNLGHPSESVFSKSFLLFTGIHWDNLN
                     SIIEIIIPTAKYFNIYSQEYAQNIAEIEGNFEEYKNNSSIIQDEEDYYLIEEESTEHP
                     DFSGIKYSREQLSDINFLQKNLYNFEGNLVLTQKELPAVNLINKDLTIEKKNNQPQIL
                     IFHTHSQEAYSDSSPGDLDEGVVGLGEKLAKELHERYGVGVLHHKGQYDVVNGKIMRE
                     GSYERMEPALRKILKDNPSIEVVIDIHRDGLPDDVRLVTNINGKPTAKVMFVNGICKT
                     MLNNKLVDIKSLPNPYIEENLAFSLQMQLKGNELYPGLLRKIYIKPYRYSLHMCPKSL
                     MIEVGANTNTYEEAENAIEPLCEILAEVLGLEESNY"
     gene            complement(4489..5502)
                     /gene="gpr"
                     /locus_tag="PLG49_10110"
     CDS             complement(4489..5502)
                     /gene="gpr"
                     /locus_tag="PLG49_10110"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOQ17697.1"
                     /translation="MSEKSKELEAPVFNIRTDLAIETREMIRKEQDVEIPGVKVNIEE
                     DREKQIKVSWVEVLDAEGEKQMGKPVGNYVTIESPMMKENDIDAHEEIIKILAKQLVK
                     ILNLDDDAFILVVGLGNWNITPDALGPKVVSKIFVTRHLLDHIPEQVDESVRPVSAIA
                     PGVMGLTGIETSEIIEGVVKKIKPDLVIAIDALASRKTSRVNTTIQIADTGVHPGSGV
                     GNSRRGLNEETLGVPVIALGVPTVVDAATLVNDTMDHLIDAMLKEANPDKEFYKMLKN
                     LNEQEKYQLIREVLNPYVGNLFVTPKEIDSVIDRLANIIANAINIALHPGIDLKDINR
                     FTY"
BASE COUNT         1669 a         1134 c          789 g         1992 t
ORIGIN      
        1 agatattatt taattattta tggtaaaaat tctgcaaata cgatattggc taaatccagc
       61 cctttcaggc tcaattgaat acggtcttgt ttttttatta aaagtccttc ttcaataagc
      121 tttttgacct tttctccaaa aagtgcatga aaggaaatat taaattggct ttcaaagtct
      181 tttatcgata tcccttcagt aagacgcagg cccaaaaaaa tatactctcc atattgcata
      241 gttaaaggaa tgctttcaat ttcttcttta atgctctcga tttttccttg actttgaata
      301 tatttatcta aattatagct attgtgataa cgctgaccat ttataaaaga atgagctccc
      361 agacccattc ctacataagg cttgtatttc caacaattca tattatgcct gctttcaaat
      421 ccttttttgg aaaaattcga aatttcatag tgatgatatt tattttctgt taagattttt
      481 cttgcataat ggtacaattc tctatctagc tcatcggaag cttcttttag cttaccttct
      541 ttttttaatc ttccgaaata gctgttttct tcaataatta agctataaca agaaatatgc
      601 tctggttcgc tttttatgat atattttaaa gtatcggccc attctttttt agtctggtca
      661 ggaagagaaa aaattaagtc tacattgatg ttttgatagc ccatttgtct ggccaagtga
      721 taattgttat aaaaatcttc cagactatga atcctgccaa gactttttaa aatcttattt
      781 tgagtggcct gaagccccat actcaaacgg ttcacacgat attctttcag taaatagagc
      841 agttctttgt ttaaggtacc cggattactt tctacagtaa attcaaaaga agattctatt
      901 ttaaaatttt ccttcagact ttttaatatt ttctccaata aagcaagagg aagaacggtt
      961 ggagttcctc ctcctataaa aatcgtatcc acttgcctgt cttgaagaat ttccccatat
     1021 tgctttattt cttcgcataa agcaaaaaca tagggaacca tcttttcatt ttgattgtcg
     1081 aaagaagtga aatcacaata ataacatttt gctttacaaa aaggtatatg aatatataat
     1141 cctacacatt tgtccatagg ctctactcca attttaaaac actcataaaa gcagattgag
     1201 gaacttctac actgccaatc tgacgcattc ttttctttcc ttctttttgc ttttccaaaa
     1261 gctttttctt acgggtaatg tctcccccgt aacattttgc taagacgtct tttcttaaag
     1321 cactaatggt ttctcttgca ataattttat tgccaatagc cgcctgaata ggaatttcaa
     1381 ataaatgcct tggtatttcc tgctttaatt tttctgcaat ttttctgccc cgttcataag
     1441 ctttgtcctt atgaacaata aaacttaagg catcaactac ttctctgtta accaaaatat
     1501 ctaacttaac caggtcagat tcctgatagc caattaattc ataatcaaaa gaagcataac
     1561 ccctggttct tgattttaat gcatcaaaaa aatcatagat aatttcatta aggggcagat
     1621 gataagtcag tacagccctg gtctcttcaa gatagtccat tccaatgtat tctccccgtc
     1681 tttcctggca tagttccatg actgaaccaa tatattccgt aggaacaata atctctgcct
     1741 taacaacggg ttcttccata tattttattt ccgaaggatc cggtaaatcc gttggattag
     1801 acaattcaat gatttcatca ttggttttat gcactttata tataacgctg ggtgcagtcg
     1861 tcaccaggtc taaattatat tctctttcta aacgttcctg aataatttcc atatgcagca
     1921 aacctaaaaa accacatcta aaaccaaaac ctaaagcaac agagttttct ggttcaaata
     1981 ctaaagccgc atcatttaat tgaagttttt ctaaagcttc tcttaaatct tcatattttg
     2041 ccccatctgc cggatacata ccgcaataaa ccatgggatt gactttctta taccctggca
     2101 aaggctcaga ggctggatta tctgcatcgg taatggtatc tcctactctg gaatctttta
     2161 cattttttat gctggccgta atataaccta catctccagc cgttaattca ttcgttacaa
     2221 taaattggcc cggaccaaaa acgccaactt cattaacttc aaattctttt cccgtagaca
     2281 tcattctaat ccgcatatct ttttttattt tgccttcttt gattcttata aaggcaataa
     2341 cgcctttata agaatcatat acagaatcaa aaattaatgc ctttaaaggt gcattttcat
     2401 caccttgcgg acttggaatc tcttttacaa ttttctctaa aacagcttct atattgatgc
     2461 ccattttagc tgaaatcaaa ggggcatctt ctgcaggaat tcctatgata tcctcaattt
     2521 ctctttttgc attttcggga tttgcactgg gtaaatcaat tttattaatg acggggacaa
     2581 tttctaaatt atgctctatt gccaaataga cattagccaa agtttgtgcc tcaatcccct
     2641 gggctgcatc tacaactaaa acagcacctt cacacgctgc caggctcctg gaaacctcat
     2701 aattaaaatc gacatggccg ggtgtatcaa tcagattaaa tatgtattct tctccatctt
     2761 ctgctttata taccaatctt acggcctgtg attttatcgt tattccccgt tccctttcca
     2821 aatccatatt gtccaatatt tgatcctgca tttctctttc cgttaataaa cctgtctttt
     2881 gaattatacg atccgccaaa gtagactttc catgatcaat atgagcaata atacaaaaat
     2941 ttcgagttct ttcctgttta gaaaggacca tataatgtgt tcctccttac cataatctaa
     3001 aagcaaatct ataaagaaaa cagacttttc caataaaagg ggaatacaag tagttaaaag
     3061 ctacttgtat tatccaagat tatatcatat ttttaatact tcgatatatt ataacacagg
     3121 ccaacttaag gctcaatagt ttgattcttc aagtcctaaa acttccgcca gaatttcaca
     3181 taaaggctct atggcatttt ctgcttcctc gtaggtattg gtatttgctc caacttctat
     3241 cattaaactt ttcggacaca tatgtaagga ataacgataa ggcttaatat aaatttttct
     3301 aagcaacccc gggtacaact cattcccttt aagctgcatc tgcaaactga aagctaaatt
     3361 ttcttcaata taaggattgg gaagactttt aatgtcaacc agcttattgt taagcatggt
     3421 tttacatatc ccattgacaa acatcacttt ggcggttggc tttccattaa tattcgtcac
     3481 caagcggacg tcatccggaa gtccatccct gtgtatgtct attaccactt caattgaagg
     3541 attatccttt aaaatttttc tcaaagcagg ttccatccgt tcataactgc cttctctcat
     3601 tatttttcca ttaaccacat catattgtcc tttgtgatgt agtacgccga ctccatatct
     3661 ttcatgaagt tctttagcca atttttcccc aagtccgaca actccttcgt ctaaatctcc
     3721 cggactactg tctgaatatg cttcctggga atgggtatga aaaataagaa tttgaggctg
     3781 attgtttttt ttctctattg ttaaatcttt gttgataaga ttaaccgcag gtaattcttt
     3841 ttgagttaaa actaaattcc cctcaaaatt atataagttt ttttgcaaaa aattaatatc
     3901 tgacaattgt tctctgctat atttaatacc tgaaaaatca ggatgctctg tactttcttc
     3961 ttctataaga taatagtctt cttcatcttg aatgattgaa gaattatttt tatactcttc
     4021 aaaatttcct tctatttctg cgatattttg agcatattcc tgactataga tattaaaata
     4081 ctttgccgtt gggataataa tttctataat gctgtttaaa ttatcccagt gaatccccgt
     4141 aaatagtaaa aaagatttgg aaaaaacact ttcggaagga tgtccaagat ttgaatagtc
     4201 ttctacgtaa ggtattgttt cctccagtat tttcttgtaa gtccttgaat ctaaagtttg
     4261 gtccttcaga tattttgtaa tctgcctgtc caaatgaata tcacctgtaa gctttttatc
     4321 ttcttgcagc ttaataatac ctattacaag aagtaaaagc aggccaatcc ataattgtgt
     4381 cttaattttt tttaaactga ttgtatgaat cctaaccaaa aaaattgccc cccaataata
     4441 tgaattgata ttatatatat tattaaagaa gggggaatta tatgataatt aataagtaaa
     4501 gcggttaata tcttttaaat caattccggg atgaagggca atattgattg cattggcaat
     4561 aatattggcc agtctgtcaa taacactgtc aatttcttta ggtgtaacaa ataaatttcc
     4621 aacataagga tttaatactt ctctgatgag ctggtatttt tcctgctcat tcagattttt
     4681 taacatttta taaaactctt tatcaggatt agcctctttt aacatggcat caattaaatg
     4741 atccattgta tcattaacca aagttgctgc atcaacgacc gtaggtactc caagagcaat
     4801 aacaggaacg cctaaagttt cttcatttaa tcccctgcga ctgtttccaa cgcctgaacc
     4861 aggatgtact ccagtatctg caatttgaat agttgtatta acacgactgg ttttcctgga
     4921 agccagggca tcaatcgcaa tcaccagatc cggctttatt tttttgacaa ccccttcaat
     4981 gatttcactg gtttcgatgc ctgttaaacc cattacacca ggagcaatag cacttacagg
     5041 acgaacggat tcatccacct gttcaggaat atgatccaac aaatgacgtg tcacgaaaat
     5101 ttttgacact actttgggac caagggcatc tggcgtaatg ttccaattgc ccagacccac
     5161 cacaagaata aaggcatcgt catctaaatt taaaattttg accagttgtt ttgctaatat
     5221 tttaataatt tcttcatggg catcgatatc attttctttc atcatgggac tttcaatggt
     5281 aacataatta cctactggtt tgcccatctg cttttctcct tcagcatcca atacttcaac
     5341 ccaactcact ttaatctgtt tttccctgtc ttcttctatg tttactttaa cgccaggaat
     5401 ttctacatcc tgttcttttc ttatcatttc cctggtctct attgctaagt cagtacggat
     5461 attaaatact ggtgcttcca attcttttga tttttctgac atattaccaa ctccctgttc
     5521 tttaataaaa gatatgatat tgcgacataa aagtattttt tccagttatt tttcttatat
     5581 tcat
//
LOCUS       DAOSUD010000109         5530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6657_length_5530_cov_21.307763, whole genome shotgun sequence.
ACCESSION   DAOSUD010000109 DAOSUD010000000
VERSION     DAOSUD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_5530_cov_21.307763"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..992)
                     /locus_tag="PLG49_10115"
     CDS             complement(105..992)
                     /locus_tag="PLG49_10115"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOQ17698.1"
                     /translation="MNNNLDELKRKYEEIEVPQNTLELVEKTIKKAKKKEKALNIFKD
                     FGATLVAIVAILTILVNLNSNIAYGLSKLPILNVVVEVVNFRNYESKHKDMEANLNIP
                     EISVKDEEGKILEESTKKINASIEEYTNKLISRYQEEVEATGGEGLQTVNLDYEIITN
                     NERLFSIRFNELIVMASGNEKVKIFHLDKQTGEVIILKDLFREDANFKKIISDNIKEQ
                     MKKRMEEDENLTYWIDSEVPEWDFKSINDDTTFYINEKGKLVIVFDEYEVAPGFMGVQ
                     EFEIPTEIIKDFAKDGFIS"
     gene            complement(985..1476)
                     /locus_tag="PLG49_10120"
     CDS             complement(985..1476)
                     /locus_tag="PLG49_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ17699.1"
                     /translation="MASKKMKMYVEEEILKSYKSMYRLAYTYVKNEADALDVVQESVY
                     KAIRNASKIKHYEYIKSWLYRIVINTSLDILHKRNKESLVEKMTEEIKEETNRDLDLI
                     NSLQNLTDKERLVIVLRFFEDMKINDIAGVLHENPNTIKSILYRALKKLRVDMEKGGY
                     KNE"
     gene            complement(1718..2950)
                     /locus_tag="PLG49_10125"
     CDS             complement(1718..2950)
                     /locus_tag="PLG49_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOQ17700.1"
                     /translation="MSKSTQTAVSTQEKIILGLQHVLAMFGATVLVPMLTGLDPAIAI
                     LSAGLGTLVFHLCTKGMVPVFLGSSFAFINAIALVLKDEGLGAVKGGIIVAGLIYVLM
                     AGIIKLFGVEKVRSFFPPIVAGPIIMVIGLNLSPTAINNSLFNQGSFDLKYLLVSSIV
                     LITMIVVSIFAKGFFKLVPILIAVAVGYLVSLPLGLVDTAVIADARWFGIENFEQIIT
                     LPEFTLSGVLAIAPIALVVFIEHIGDVTTNSAVVGKDFFKEPGVHRTMLGDGLGTLVA
                     GLIGGPANTTYSENTGVLAVTKVYDPAILRIAAVFAIIISMLGKVGAIISCIPTPVMG
                     GISIVLFGMIASVGVRILIEAKLDFSYSRNLLIAAVILVCGIGIESIPVYGNLRLSGL
                     TIAAILGVALNKLLPENI"
     gene            complement(2976..3518)
                     /gene="pyrR"
                     /locus_tag="PLG49_10130"
     CDS             complement(2976..3518)
                     /gene="pyrR"
                     /locus_tag="PLG49_10130"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOQ17701.1"
                     /translation="MTEYKILLDEKAIQRALTRISHEIIERNKGVDKLLLIGIKTRGI
                     PLAQRIAQKIFEVENVQVPVDTIDITFYRDDLKHRSIQPVVHNTNLKHDVNDKTVVLV
                     DDVIYTGRTVRAAIDALIDAGRPQFIQLAVLIDRGHRELPIRPDFVGKNIPTSKSEIV
                     RVLLDETDNQNMVILEKTMP"
     gene            complement(3844..4773)
                     /locus_tag="PLG49_10135"
     CDS             complement(3844..4773)
                     /locus_tag="PLG49_10135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ17702.1"
                     /translation="MDFFEFFVEAEDAGKRIDVYLSEKIENCSRSYIQKLINEKRIQV
                     NQKATRSNYKLKHKDLLYVEIPEAEPIEIQAEDIPLDIVYEDEDVIVINKAQGMVVHP
                     AAGHYSGTLVNALLFHCRDGLSGINGSLRPGIVHRLDKDTSGILIAAKNDRAHQELTI
                     QLKERAIVRKYHAIVYNNLVEDYGTINAPIGRDPKDRKKMAVTVKNSKEAITHFRVLE
                     RFDDFTYIEARLETGRTHQIRVHMKHIGHPVLGDDTYGPKKQPFKLNGQMLHARFLGF
                     YHPSTREYMEFEAPLPDYFNELLNLLRNREKKL"
     gene            complement(4867..5469)
                     /gene="lspA"
                     /locus_tag="PLG49_10140"
     CDS             complement(4867..5469)
                     /gene="lspA"
                     /locus_tag="PLG49_10140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ17703.1"
                     /translation="MDDLYYVFCFEGERKLVVTFILAVLSLVALDQFSKYKAVVHLKE
                     MRTFPIIQNVFHFTYVENEGAAFGILQNKRLFFIMITLVVLLGISYYYLKLPKKKPYN
                     RIRLCLILIFSGALGNLIDRIRLGYVVDFFDFRLINFPVFNVADVCVVIGTIFLSLLI
                     LFLTEDEMEVGKTKIEKAADTHKEELFKDEIKMISGDEKQ"
BASE COUNT         1866 a         1116 c          654 g         1894 t
ORIGIN      
        1 aagaacctat gaccgttcca tagaaaacct gtataatgat tataaaaaca gataaaaaaa
       61 cagctgcagt aatggatata ctgcagctgt ttttttaata tattttaact tataaaacca
      121 tcttttgcaa agtctttaat tatttctgtt ggaatttcaa actcctgtac gcccatgaaa
      181 cccggagcga cttcatattc atcaaaaacg attactagtt ttcctttctc attgatataa
      241 aaagttgtat catcattaat acttttaaaa tcccattctg gaacttcact atctatccaa
      301 taggttaaat tttcatcttc ttccatgcgt tttttcatct gctctttgat attatcggat
      361 atgatctttt taaaattagc atcttctcta aaaagatcct ttaatataat cacttctccg
      421 gtttgcttat ccagatgaaa aattttaacc ttttcattgc cgctggccat gacaatcagc
      481 tcattaaaac gtatagaaaa taatctttca ttattcgtaa tgatttcata atctaagttt
      541 actgtttgta agccttctcc tcctgtagcc tccacttctt cctgatatct ggaaattaat
      601 ttattggtat actcctctat ggatgcattt atctttttag tactctcttc caatattttc
      661 ccttcttcat cctttacact tatttctggt atattaaggt ttgcttccat atctttatgt
      721 ttactttcat aattgcgaaa atttacaact tcaacaacaa cattaagtat aggaagcttt
      781 gaaagaccat aagcaatatt ggaatttaaa ttaacaagaa ttgtcagaat agctactata
      841 gctaccagag ttgcaccaaa atctttaaaa atatttaatg ctttttcttt ctttttggct
      901 ttttttatag ttttttcaac aagttccaaa gtattttgag gaacttcaat ctcttcgtat
      961 tttcttttta attcatctaa gttattattc atttttataa cctccttttt ccatatcaac
     1021 ccgtaatttt ttcaatgccc ggtaaagaat tgatttaata gtattcggat tttcgtgtaa
     1081 aactccagca atatcattaa ttttcatgtc ttcaaaaaac ctcagcacta ttacaagacg
     1141 ctctttatct gtcagatttt gcaacgaatt tataagatct aagtctctgt ttgtttcttc
     1201 tttaatctct tccgtcattt tctctactaa actttcttta tttctcttat gtaatatatc
     1261 taaagaggtg ttaattacta tcctgtatag ccaggatttt atgtattcat agtgcttaat
     1321 tttcgacgca tttctaatgg ccttatatac actttcttgt accacatcta aagcatctgc
     1381 ttcatttttc acataagtat aagctaagcg gtacatggat ttataggatt ttaatatttc
     1441 ttcttcaaca tacattttca tcttctttga agccattgag gtcctccttt ctatccataa
     1501 gaccggctaa tcgtttaaaa agtttcaata aatgcttatt tttttgcata acaaaaacac
     1561 ctctgttcat tttattaaca gagatgtttt aattaaaaaa attgccacaa gaaaacacca
     1621 gctataaata aaccataaat atttaaaatt gatcatttaa aagaaatata cagttctaat
     1681 gtatattcac tgacaacata tcaaacacat tttagaatta tatattttct ggtaacaact
     1741 tatttagagc gactcctaaa attgccgcaa tcgtcaaacc tgaaagcctt aagttcccat
     1801 aaacaggaat actctcaatc cctattccgc ataccaaaat aacagcagca atcaaaagat
     1861 tacgactata tgaaaaatct aactttgctt caattaaaat tctaaccccg acactggcaa
     1921 tcatcccaaa taaaacaata ctaattccac ccattactgg tgtcggaata caactgataa
     1981 ttgcacctac ttttcccaac atactaatga taattgcaaa aactgctgct attctaagaa
     2041 ttgcaggatc atataccttt gtaactgcta aaacaccagt attttcgcta taagtcgtat
     2101 ttgccggtcc accaattaaa cctgccacaa gtgtacctaa accatcacct aacattgtcc
     2161 tgtgaacacc aggttcttta aagaaatctt tacctactac agcactattc gtggtaacgt
     2221 ccccaatatg ttcaataaat acaaccaaag caattggtgc tattgctaaa accccactta
     2281 atgtaaattc tggtaaagta ataatttgtt caaaattttc aatgccaaac catcttgcat
     2341 cagcaatcac ggctgtatcc accaaaccta aaggtaaaga aaccagatat ccaacagcaa
     2401 ctgctattaa tattggcacc agtttaaaaa atcccttagc aaaaatagaa acaacaatca
     2461 ttgtaataag aacaattgaa gaaaccagca aatattttaa atcaaatgaa ccttgattaa
     2521 acagactgtt attgattgcc gtaggactta aattaagacc aataaccata ataatcggtc
     2581 ctgcaacaat tggtgggaaa aaggaacgta ccttttcaac accaaacaat ttaataattc
     2641 ctgccataag gacatatatt aaacctgcaa caattatacc accttttaca gctcctaaac
     2701 cttcatcctt aagaaccagt gctattgcat taataaaagc aaaactagaa cccaaaaaaa
     2761 caggtaccat ccccttggta cataaatgaa aaaccaatgt tcctaatcct gctgaaagaa
     2821 ttgcaatggc aggatccagg ccagtcaaca tagggactag cacagttgcc ccaaacatcg
     2881 ccaaaacatg ttgaaggcct aaaataattt tttcttgagt tgacacagca gtttgtgttg
     2941 attttgacat aaaattcctc ccaactatta tttttctaag gcattgtttt ttctaagata
     3001 accatgttct gattatccgt ttcatccagt agtaccctta caatttcact ttttgaggtt
     3061 ggtatattct taccaacaaa atccggccgt ataggaagtt ccctatgtcc cctgtcaatt
     3121 aaaacagcca actgaataaa ttgaggcctt cctgcatcaa tcagagcatc aattgctgcc
     3181 ctgaccgttc ttccagtata gataacatca tccactaaaa caaccgtttt atcatttaca
     3241 tcatgtttaa gatttgtatt atgaacgacc ggctgaatag aacggtgttt taaatcatcc
     3301 ctgtaaaaag taatatctat cgtatctaca ggcacctgaa cattttccac ttcaaatatt
     3361 ttttgagcaa ttctttgagc aagaggaatc cctctggttt taatacctat cagtaaaagt
     3421 ttatctacac ctttatttct ttctataatt tcatgagata ttctggtaag tgccctttga
     3481 atagcctttt cgtctaataa aattttatac tccgtcaaaa atacacctcc tcaacagaaa
     3541 atatcttcag aatacaatca ttctaaacca agatattctt aaatcataat acccgctaat
     3601 tgaaattttc taaaaatata acaccctcaa aaaagcttga ataattcctg aacaagtctc
     3661 ctaaaatcat tggaatccta ataaagcaaa atttatctat tgaaaaaacc cacaaaaaaa
     3721 cctcatagcg agagctagag gccataatac tcatataagc agcatatgac atccttatta
     3781 gtctctcgga ctaatttaaa ggaattttat tatttaaaat atatcacagc ttaaactaat
     3841 tgtctataat tttttttctc tattccgaag taaatttaaa agttcattaa aataatctgg
     3901 taatggcgct tcaaactcca tatactccct ggttgaagga tgataaaagc ctaaaaatct
     3961 cgcatgcaac atttgcccat ttaatttaaa tggttgcttt ttaggtccat aagtatcatc
     4021 ccctaataca ggatgtccaa tatgcttcat atgaactctt atttgatggg ttctgcctgt
     4081 ctccagcctg gcttcaatat aagtaaaatc atcaaacctt tctaaaactc tgaaatgggt
     4141 aatagcttcc ttcgaatttt taactgttac agccattttt ttacgatctt ttggatctct
     4201 cccaattgga gcattaatcg taccataatc ctcaacaaga ttattgtaaa caatggcatg
     4261 atattttctt acaatagcac gttcttttaa ttgtattgtc aattcctgat gagcacgatc
     4321 attcttagct gcaatcaaaa tgcctgaggt atccttatca agccggtgaa caattcctgg
     4381 tctcaaagag ccattaatac ctgataagcc atcacgacaa tgaaacaata atgcattcac
     4441 caaagtccca ctgtaatgac ctgctgcagg atgaaccacc ataccctgag ctttattaat
     4501 gactataaca tcttcatcct cataaacaat atctaaaggg atatcttccg cttgtatttc
     4561 aataggttct gcctcaggaa tttccacata aagcagatcc ttatgcttaa gtttataatt
     4621 tgacctggtc gctttctgat taacctggat tcttttttca ttaattaatt tttgtatata
     4681 ggatctggag caattttcga ttttttctga taaatataca tcaatccttt ttcccgcatc
     4741 ttctgcttca acaaaaaatt caaaaaaatc cattactcaa cttcctttta tttcatcatt
     4801 ataattatct gttctttttt tatattttta ccatatcctg aatgttttat atcttatctt
     4861 aaatggttat tgcttttcat ccccgcttat cattttaatt tcatccttaa ataattcttc
     4921 tttatgagta tccgctgctt tttctatttt agtctttcca acttccattt catcttcagt
     4981 taaaaataaa attaaaagac ttagaaaaat ggttccgata acaacacata catctgctac
     5041 attaaataca ggaaaattaa tcaatcgaaa atcaaaaaaa tcaacgacat aacctaaacg
     5101 gattcggtct attaaattac ctaacgcccc ggaaaaaatc aaaataagac ataacctaat
     5161 ccgattataa ggttttttct taggcaattt taaatagtaa tatgaaatac caagtaaaac
     5221 aacaagcgta atcattataa aaaataatct tttattttgc agtataccaa aagctgcacc
     5281 ttcattttcc acataagtaa aatggaatac attttgaata attggaaaag tacgcatttc
     5341 ttttaaatgt acgactgctt tgtattttga aaactgatca agtgcaacca aagacaatac
     5401 agctaatata aacgtaacta ccaattttct ctctccctca aaacaaaaaa cataatataa
     5461 atcatccatt aatcaatttt ttacatagta tttaatataa agagttcttt ctatccttat
     5521 tcgttaacac
//
LOCUS       DAOSUD010000110         5445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6774_length_5445_cov_22.565492, whole genome shotgun sequence.
ACCESSION   DAOSUD010000110 DAOSUD010000000
VERSION     DAOSUD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6774_length_5445_cov_22.565492"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..874
                     /locus_tag="PLG49_10145"
     CDS             98..874
                     /locus_tag="PLG49_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17704.1"
                     /translation="MKEKKSFLIITLTQLIFFSLTVTYFIGSDYLLPFILQYFILLIA
                     FIFLPKSKGFILLIGYITLPLLGLLFFAFSREWNLLFQFKIIYYHIISIINASLIYMS
                     AYKIRTLQNENMTLNLELEELRRYVKDSKLLTKSEFEQRSELIITAMKRRKEEGYKVY
                     FSLEKANPHIQKALFDSLTSIAVETFRKEYDLIGQISDYSFVVLLQNTNQKGMELALN
                     RYFTAVERKIDLNENKMIIDITRVGSNIEGEDKLENPVFD"
     gene            852..2066
                     /locus_tag="PLG49_10150"
     CDS             852..2066
                     /locus_tag="PLG49_10150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17705.1"
                     /translation="MKTLFLINSLVFSFILLYYTILSLFGLYYRLKKKNRPKLANYPS
                     VDLLIPAHNEGKVLQQTLEAMVKLDYPGPLHIYLLNDNSVDETEEIAEAFDKAYKNLH
                     HIRVPVGEPKGKSRVLNYGLSISDSEYFAVYDADNQPEKESLKLLVEAAENTPNAAGA
                     VGYVKTINANKNILTRLIAQEFQVFQLLMQCGRWFLFKTGSLTGTNMLVKRSIIEEVG
                     QYDVYAIAEDAELTLRITSKGKILPIVPESVTWEQEPEKLAVFIKQRTRWLQGNLYIL
                     EKIFHSFDYYKGRTLVHTFQQILIYIVFLFFLLTSHAWFIAGLFGLTTFSVKLPLLLI
                     WYASYLFYVAQLLSAQAAEESFSPLNIIIGFISYFTYSQLFLFLYFRSLFLYLKAKRK
                     KQIISWDKTLRF"
     gene            complement(2113..3063)
                     /locus_tag="PLG49_10155"
     CDS             complement(2113..3063)
                     /locus_tag="PLG49_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ17706.1"
                     /translation="MKLVSSLDFGDGHLEKLKQKFNRIDFYIYNNIEEAKEQLFDAEI
                     FVTYGFDINEELIEKMEKLKWIQCMSTGIDALPLNKLREKGILLTSVRGIHGIPIAEY
                     VMAVILNHRIEAFRFYEQQKNKIWKRKADLEELFGKTICIIGTGTIGKEIARKAKAFD
                     MKTIGVNTQGRSVEYFDELYSKADLNEALSKGDFVVQTAPLTKESYKMIGIEQFKKMK
                     PTAFFINIGRGQTVDEKALIEALEKKLIKAAAIDVFEKEPLAYDSPLWTMDNVFVTPH
                     ISGLSDMYMNRAVPILEQNLRVYLYGQGEYINKIDLEKAY"
     gene            complement(3151..3828)
                     /locus_tag="PLG49_10160"
     CDS             complement(3151..3828)
                     /locus_tag="PLG49_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17707.1"
                     /translation="MGAYFFILFAVIFLVFLNIIQIKKHSNIIIRLVCFFLFIVTGLR
                     YITLLLFTFNFPVGILKFLAYFYYGSMIGLTIPSIVALWYTIPRFRKKINEKRLLLFL
                     TPLFIFYFFMLYLQPLEIVIKTGIGYGLRLLFPWNLFVSIYYGFFVMIILLLCVSGFR
                     WYKYKFPRSQYLLFFLSYLLLLVDGLTILFNRTNTFQPFIISEAFVLIALLYAFSTKP
                     IKDKNKR"
     gene            3976..5382
                     /locus_tag="PLG49_10165"
     CDS             3976..5382
                     /locus_tag="PLG49_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ17708.1"
                     /translation="MKNKIKNLFLKRIEKIYQLLNDTQKNVLSIAWPVLVELLLGSLF
                     GMIDMIMLGRIPDPDLSAASIAAVGLTNQPLYIGLSLVQALNVGGTAITARYIGAQKT
                     DKIPNVLSHIILLTLGLLAIPLSILGIRFSEEIIHFMGAKKDTLELAVLYFRIVLIGF
                     IFQSYNFAISAALRGAGNTKTTLRVNLSVNLINVIGNAVLIYGLFGFPALGIAGAGIS
                     TTFSNILASIILTIYLLRTKGIIQLKLKNGFRLDKNIVFNLVKIGIPASLEQMALRIG
                     ILLFVKTVADLGTVVFASHQICLSILSLSFNPGQAFGIAASSLVGQNLGARNFSQAEE
                     CAKEARKMGSIISTMMAILFFIFGPQLASLYSSIPEVISNASKTLKIIAFVQPFQSSQ
                     LILSGGLRGAGDTFWPLISTFIGVLGIRLILAALFIKVFHFGLTGAWIAVFIDQFVRW
                     GIIYIRFNTGKWKTVTIQ"
BASE COUNT         1792 a          957 c          811 g         1885 t
ORIGIN      
        1 tttttattgt ttttttattg ataaaataca ttttttatta tataataata tttattgttt
       61 aatttctaaa ctatttacag catgggagag acttgacatg aaagaaaaaa aatccttttt
      121 gattataacg ttgacacaat taatcttttt tagtctaacc gtaacatatt ttattggttc
      181 ggattatctt ttacctttta ttctacaata ctttattttg ttaattgctt ttatttttct
      241 tcccaaaagc aaaggtttta ttttattaat tggctacatt acccttcccc ttttagggct
      301 tttatttttt gcctttagca gggaatggaa tttactattt caatttaaaa ttatatacta
      361 tcatattatt tctattatca atgccagctt aatatatatg tccgcctata aaatacgcac
      421 acttcaaaat gaaaacatga ccttaaatct tgaactggaa gaactcaggc gttacgttaa
      481 agattctaaa ttattaacaa aaagtgaatt tgagcaaaga agcgaactta ttattactgc
      541 catgaaaagg cgaaaagaag aaggttataa agtctatttt tctttagaaa aggcaaatcc
      601 ccatattcaa aaagcacttt ttgacagctt aacatctata gctgttgaaa ctttccgtaa
      661 agaatatgat ttaatcggac aaatcagtga ttattccttt gttgttttat tacaaaacac
      721 aaatcaaaaa ggaatggaac ttgccttaaa cagatatttt acagccgttg aaagaaaaat
      781 tgacttaaat gagaataaaa tgatcataga tataacaaga gttggctcaa atatagaagg
      841 ggaggataag cttgaaaacc ctgtttttga ttaactccct tgttttttct tttattttgc
      901 tgtattacac cattctctcc ctttttggtt tatattatag acttaaaaag aaaaacaggc
      961 caaagttagc caattatccc agtgttgatc ttttgattcc ggcccataat gaaggcaagg
     1021 ttcttcagca aactttagag gccatggtta aactcgatta tcctggcccg ctccatattt
     1081 atctcttaaa cgataattct gtggatgaaa cagaagaaat tgctgaagct tttgataaag
     1141 cctataaaaa tctccatcat attagagttc ctgttggaga accaaaagga aaatcccggg
     1201 tattgaatta tggtctaagt atttctgatt cagaatattt tgcagtttat gatgccgaca
     1261 atcagcctga aaaagaaagc ttaaaattgt tggtagaagc agcagaaaat actccaaatg
     1321 ctgcaggcgc tgtcggttat gtaaaaacca ttaatgccaa taaaaatata ctcacccgcc
     1381 tcattgctca ggagtttcaa gttttccaac ttttaatgca gtgcgggaga tggtttttat
     1441 ttaaaacggg ttctttgaca ggaaccaata tgttagtcaa acgttccatt atagaagaag
     1501 ttggccaata cgatgtttat gccattgctg aagatgctga attaacctta agaattactt
     1561 caaaaggaaa gatcctgccc atcgtccctg aatccgtaac ctgggaacaa gagcctgaaa
     1621 aattagctgt atttataaaa caacgaaccc gatggctcca aggcaattta tatatactgg
     1681 aaaaaatctt tcattccttt gattattata agggaagaac tttagtccat accttccagc
     1741 aaattctgat atatattgtc tttttatttt tcttactaac ttcccacgcc tggtttatag
     1801 ctggcctttt tggactgaca actttttctg ttaagcttcc tttactgctt atttggtatg
     1861 cttcctattt attctatgtg gcacaattat taagtgctca ggctgcagaa gaaagttttt
     1921 caccccttaa tattattatt ggttttattt catatttcac ttattcacag ctttttctct
     1981 ttttatattt cagaagtcta tttttatatc tcaaagcaaa aagaaaaaaa caaattattt
     2041 cctgggataa aaccctgcgc ttttaaaaaa gagaagctta agcttctctt ttttacatca
     2101 gtaaaaattt tactaataag ccttttctaa atctatttta ttgatatatt ctccctggcc
     2161 atataaataa actcttaaat tttgttctaa aataggtact gcacggttca tatacatatc
     2221 tgacaggcca gaaatatgag gcgtcacaaa aacattatcc atggtccata aaggactgtc
     2281 ataagctaaa ggttcctttt caaatacatc gatagcagca gcttttatta actttttctc
     2341 taaggcttca attaaagctt tttcatctac agtttgtcct cgtccaatat taataaaaaa
     2401 agcagtaggc ttcatttttt taaactgttc aatgcctatc atcttataac tttcttttgt
     2461 taaaggtgca gtttgtacaa caaaatcccc ttttgacaaa gcctcgttta agtcagcctt
     2521 cgaataaagt tcatcaaaat attcgacact cctgccctga gtattaaccc caatggtttt
     2581 catatcaaaa gctttggctt tccttgcaat ttcttttcca attgtgcctg tcccaataat
     2641 gcatatcgtt ttaccaaaaa gctcctctaa atccgctttt cttttccaaa ttttattttt
     2701 ctgctgttca taaaatctaa aggcttctat gcggtgattt aatatgaccg ccatgacata
     2761 ttcagctatt ggaatgccat gtatgcctct cacactggtc aataaaattc ctttttctct
     2821 aagcttattt aagggaaggg catcaatacc tgtagacata cactgaatcc attttaactt
     2881 ttccattttt tcaataagct cttcgtttat gtcaaaacca taagtaacaa aaatctctgc
     2941 atcaaataac tgttcttttg cttcttctat attattatag atataaaaat caatcctgtt
     3001 aaacttttgc tttaactttt ccaaatgtcc gtctccaaaa tcaagcgaac taacaagctt
     3061 catagcagca ttcccccatc ataattttta agtaacaaat acattcttta ttattttctc
     3121 attgattata atcaatatga ctaaatatgt ctatcgcttg tttttatcct ttatcggttt
     3181 tgttgaaaaa gcatataaga gagcaatcaa tacaaatgct tccgaaataa taaagggctg
     3241 aaaagtattt gttctgttaa ataatatagt caatccatcc actaagagca ataggtaact
     3301 taaaaaaaac aataaatatt ggcttcttgg aaatttatac ttataccaac gaaagcctga
     3361 tacacataat aataagatta tcattacaaa aaacccataa taaatactta caaaaagatt
     3421 ccagggaaat aataaacgaa gaccatatcc tattcctgtt ttaataacga tttctaaagg
     3481 ttgcaaataa agcatgaaga aataaaaaat gaataatggc gtcaaaaaca acaaaagtct
     3541 tttttcgttt atttttttcc taaaacgggg aatagtatac cacaaagcaa ctatggatgg
     3601 aatcgtaagc ccaatcatag aaccataata aaagtaagca agaaatttca gtataccaac
     3661 tggaaaatta aaagtaaaaa gcaataaggt aatatatctt aatcctgtaa caataaataa
     3721 gaaaaagcat accagacgga tgataatatt ggaatgcttt ttaatttgta tgatattaag
     3781 aaacactaaa aaaattacag caaaaagaat aaaaaaatat gcccccatat tccccctcct
     3841 gttctttata ctattttatg tctctggaac tcagaccatg caaagaatac ggaaaagctg
     3901 gcataatgaa taactttatg atacaatgat tccctggaac acttgcgcaa aaataataac
     3961 agaaagaaga ttaatatgaa aaataaaatt aaaaatttgt ttttaaagag aattgaaaaa
     4021 atctatcaac ttttaaacga tacacaaaaa aatgttcttt ctatcgcatg gcctgtatta
     4081 gtagaattac tgctaggctc tttatttgga atgatagaca tgattatgct gggacgtatt
     4141 cctgatcctg atctgtctgc tgcatccatt gcggcagtgg gtttaaccaa ccagccctta
     4201 tatataggtc tttctctggt tcaggctctt aacgtaggag gtacagcaat tactgcacgc
     4261 tatatcgggg ctcaaaaaac cgataaaatt ccaaatgttc tgagtcatat tattttatta
     4321 accttaggtc ttctggcgat tcccctttcc attctcggca tccgtttttc ggaagaaatc
     4381 atacatttta tgggggctaa aaaagatact ttagaattag ctgttttata ttttagaatt
     4441 gttttaattg gttttatctt tcaatcttat aattttgcca tttcggctgc cttaagaggt
     4501 gccggtaaca ccaaaaccac cttgcgagtc aatctgagtg tgaacttaat taatgtcatt
     4561 ggaaatgctg ttttaatcta tggtcttttt ggatttcctg ccttaggtat tgccggggct
     4621 ggtatttcta ctactttttc aaatattctt gccagtatta tcctgaccat ttatctttta
     4681 agaacaaaag gaattataca actaaaatta aaaaatggat tccgactgga taaaaatata
     4741 gtattcaact tggtaaaaat cggaattcct gcatctttag aacaaatggc tttaagaatt
     4801 ggaatattgc tatttgtaaa aaccgttgca gatcttggta ctgtagtatt tgcttcccat
     4861 caaatatgtt taagcatctt aagtttatcc tttaatcctg gacaagcttt tggtattgct
     4921 gcttcttccc tcgtaggcca gaatcttggg gcaagaaact tttcccaggc agaagaatgc
     4981 gcaaaagaag caagaaaaat gggctcaatt atttctacaa tgatggctat tcttttcttt
     5041 atctttggcc ctcaactggc ttccttatat tccagtatac cagaagttat ttccaatgct
     5101 tctaaaacat taaaaattat cgcttttgta cagccttttc agtcttctca attgatttta
     5161 tccggtggac tcaggggagc tggtgatact ttttggcctc ttatatcaac tttcattggg
     5221 gtcttaggaa tccggctgat tcttgcggct ttatttatca aagtatttca ttttggtctt
     5281 acaggtgcat ggatagcagt atttattgac caatttgtcc gctggggaat catctatatc
     5341 cgctttaaca ctggaaaatg gaaaacggtt actattcaat aaaatagtaa ccgttttttg
     5401 tttaaaagtt aaaacttatg ttcttaaatc atttttataa tcgat
//
LOCUS       DAOSUD010000111         5406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6823_length_5406_cov_27.905251, whole genome shotgun sequence.
ACCESSION   DAOSUD010000111 DAOSUD010000000
VERSION     DAOSUD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6823_length_5406_cov_27.905251"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLG49_10170"
     CDS             <1..592
                     /locus_tag="PLG49_10170"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ17709.1"
                     /translation="INSWIMTIEDSEIIPFGTIHPGYEDWKNEIRRLASMGIKGIKFH
                     PDYQNFYVDDPKLFKIYEEIFSHNLIALFHAGIDIGLPEPCHCTPGRLKNLINHFKGA
                     VIIAAHMGGYDCWDDVEKYLLGEEIYFDTSYSSHLLEPELMTRLIKEHGVNKILFGTD
                     SPWGHQKKEIEFIHKLSLTEEEKLAILGKNAEKLLK"
     gene            632..2548
                     /locus_tag="PLG49_10175"
     CDS             632..2548
                     /locus_tag="PLG49_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOQ17710.1"
                     /translation="MNVNAMIHTNDNCIGCNRCIAFCPIPDANKAIEINGKNAIHVDG
                     EKCIGCGNCMEACMHDARDYHDDTRKFFNDLKNGKSISLIVAPSIRTNFINEYKNLFG
                     YLKSLGVHWIYDTSFGADITTWAYLKYIKERRLTGTISQPCPVVVNYIEKYKPELLEK
                     LAPVHSPMMCTAIYMRKYKRIDDAFAFISPCIAKKNEIEDPNTYNLIQYNVTFKKLRE
                     YLRQNQVKLSDYPPYDFDGMEGELGAVFPKHGGLRENVEFHLGGKGWIRQIEGQKEAY
                     KYLREYGKRIKQPKELPILVDILNCRHGCNFGTGTTKEPTVDDVDLILHYKKMQSTRD
                     KKGKLKKNYDLFKMFDKKLKASDFERHYSNKFIKLYEVSEEEIEAAFAKLNKTTDEDR
                     QINCTACGYESCLNMAEAIAKGNNYEKNCIHYNKKLVELEKEEITKKNNEIEEMFNRV
                     FKMSEERKRASEELKKDIEAISQALEQVTKASEETARVLDKISGETNDVVEQVHELKR
                     IVDLINKNINKYVENTQVIVSISEQTNLLALNASIESARAGEQGRGFAVVAEEVRKLA
                     EQTKLTAETAQMNNISTLPNLEKIISMSESFLSKMEEINEAIQSIAASTEEITSQSEE
                     IASTAAAIINKNDV"
     gene            2679..3458
                     /locus_tag="PLG49_10180"
     CDS             2679..3458
                     /locus_tag="PLG49_10180"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17711.1"
                     /translation="MTPIVELKSVAKTYHTPVGETKALENLSLTINKGEFISIVGPSG
                     CGKSTILSLIAGLIQPSDGEVLVFGSPVTGTSSKIGYMLQKDHLFEWRSILSNVYLGL
                     EIQKKLTDENKAYVNKLLDIYGLADFKNRFPSELSGGMRQRVALIRTLAIRPEILLLD
                     EPFSALDYQTRLIVSDEIGRIIKKESKTAILVTHDIAEAISMADKVVVLTERPGKIKA
                     SHDIKLSIDNRTPFKSREAPEFREYFNIIWKELKTHESESY"
     gene            3439..4251
                     /locus_tag="PLG49_10185"
     CDS             3439..4251
                     /locus_tag="PLG49_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17712.1"
                     /translation="MKVKAINYSKDHLKYLSAIKRREKIIKASQIFILVSFLLLWEMA
                     ANFGWIDAFIFSQPSKILKTLWEMALDGSIFLHTGISLFETVAGFLLSTLLGTSIAVL
                     LWWNNFILRVTDPYLVILNSLPKTALAPILIVWMGNNMKSILFTAVLMSIVVTALTVL
                     NGFQEVDQDKIKLIESFGGNKKDVLIKVMIPANVPTIINALKVNVGLSLVGVMVGEFL
                     VAKAGLGYLIIYGSQIFKLDWVMLSIVVLAFLAALLYKMVLILERRFLKWRE"
     gene            complement(4317..5243)
                     /locus_tag="PLG49_10190"
     CDS             complement(4317..5243)
                     /locus_tag="PLG49_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOQ17713.1"
                     /translation="MNFQSIFNQILILFLLLVIGYGAKKRNVFDDSTIKGISSILVKL
                     TLPALIINSLTQEQATAQSIKESGLILLLSFFIYLLSFIIALLVPKLIASKKEETGIF
                     EFTLLFSNVGFMGYPVVQAVLGDEALFYVSFYNLPFNVFVFTIGLWMLTKGKKHQKKF
                     DWKLFINTGTISVIIGFILFLFSIPIPAPITGCFDILGSMTTPLSMLVIGGLLANTES
                     KYLFSNWRIYVISFTRLIIIPLIIYGMLSPFVYHKYLLGVPVIIGAMPAAANTAILAS
                     EYGANSELASRIVFISTLFSVITIPLISLLLI"
BASE COUNT         1967 a          729 c         1048 g         1662 t
ORIGIN      
        1 tattaattca tggataatga ctatagaaga ttcagagatt ataccatttg gaaccattca
       61 tcctggatat gaggactgga aaaatgaaat cagaagactg gcttctatgg gaattaaagg
      121 cataaaattt catccggatt atcaaaattt ttatgtagat gaccctaaat tatttaaaat
      181 ttatgaagaa atatttagtc acaatttaat tgctttattt catgcaggga ttgatatagg
      241 cttaccagag ccttgtcatt gtacacccgg aagattaaaa aatttaatta atcactttaa
      301 aggtgctgtc attattgcag cacatatggg ggggtatgat tgttgggatg atgtagaaaa
      361 gtatttatta ggagaagaga tttattttga tacttcttat agttcgcatc ttcttgaacc
      421 agaattaatg accagactaa taaaagaaca cggggttaat aaaatattat ttggcacgga
      481 ttcgccgtgg ggacatcaaa aaaaggaaat tgaatttatt cataagcttt ccttaactga
      541 ggaagaaaaa ttagctattt tagggaaaaa tgcagagaaa ttattgaaat aatagcttct
      601 cttgcaataa atatatgtag ggggaacgag aatgaacgtg aatgcaatga ttcatactaa
      661 tgataattgt attggctgta accggtgtat tgctttttgc ccaattcccg atgccaataa
      721 ggcaattgaa atcaatggga aaaatgccat ccatgttgac ggggaaaaat gtattggttg
      781 cgggaattgt atggaagcct gtatgcacga tgccagggat tatcatgatg ataccagaaa
      841 atttttcaat gacttaaaga acggtaagag tatttcactc attgtagcgc catctatcag
      901 gaccaatttt ataaatgagt ataaaaattt atttggttat ttaaaatctt taggtgttca
      961 ttggatatat gatacttctt ttggagcgga tattacaacc tgggcatacc tgaagtatat
     1021 taaagaaaga agactgacgg gaaccatttc tcaaccttgc cctgtagtgg ttaattacat
     1081 agaaaaatac aaaccagaat tattggaaaa attagcacct gtacatagcc cgatgatgtg
     1141 tacggcaata tatatgagaa aatacaaaag aatagatgat gcttttgctt ttatttcccc
     1201 atgtattgca aaaaagaatg aaatagaaga tccaaatact tacaatttaa tacaatataa
     1261 tgttactttt aaaaaattac gggagtatct aagacaaaat caagttaagc tttctgatta
     1321 tcctccttat gactttgatg gtatggaagg agaactgggt gcagtatttc ccaagcatgg
     1381 cggacttagg gaaaatgtag aatttcattt aggcggtaaa ggctggatcc gtcaaattga
     1441 aggtcaaaag gaagcttata agtatttaag agaatacgga aagagaataa agcaacctaa
     1501 ggaattgcct attctggtag atattttaaa ctgcagacat ggctgcaatt ttgggacagg
     1561 aacaacaaaa gagccgactg tagacgatgt ggatttaatt cttcactata agaagatgca
     1621 aagtaccagg gataaaaaag ggaaattaaa aaagaactat gacttattta aaatgtttga
     1681 taaaaaatta aaagcttcag attttgaaag acactacagt aataaattca taaagcttta
     1741 tgaagtcagt gaagaagaaa tagaagctgc ctttgctaaa ttgaacaaaa cgactgatga
     1801 agaccgacag attaattgta cagcttgtgg atatgaatct tgcttgaata tggcagaagc
     1861 aattgccaaa ggtaataatt atgagaagaa ttgtatccat tataataaaa agttagttga
     1921 gttggaaaaa gaagagatta caaaaaagaa taatgaaata gaagaaatgt ttaacagggt
     1981 attcaagatg agcgaagaaa gaaaaagagc cagtgaggag ctaaaaaaag atatagaggc
     2041 aatatctcag gctttagaac aggttactaa agcaagtgaa gagactgcaa gagtattaga
     2101 caaaataagt ggtgagacca atgatgtagt tgaacaagtc catgaattga aaagaattgt
     2161 ggatttaatt aataaaaata ttaataaata tgttgaaaat actcaggtta ttgtaagtat
     2221 ttcagaacaa accaacttat tagctttaaa tgcttctata gaatccgcaa gagccggcga
     2281 acaaggaaga ggttttgctg ttgtagcgga agaagtaaga aaactggcag aacagaccaa
     2341 attgactgca gaaacagcac aaatgaataa tatttctact ttgcccaatt tagaaaaaat
     2401 tatcagcatg tctgaatcat ttttatcaaa aatggaagaa atcaatgagg cgattcaaag
     2461 tattgctgca agtactgagg aaattacttc tcagtcagaa gaaattgcat caacagctgc
     2521 agcaatcatt aataaaaatg acgtttaaaa aattatcatt atttaaaaag ctgtcttttt
     2581 caagtaaaga cagctttttt atttcggaat ttgtcacatt taatcgcata ttttaatata
     2641 ctaattattg aaagtgccca ttttaaggag gctggtttat gactcctatt gtagaactta
     2701 aatcggttgc caaaacttat cacactcctg tgggagagac taaagcattg gagaatctta
     2761 gtcttacgat taataaagga gagtttatca gtatagtagg tcccagtggt tgcggaaaat
     2821 ctaccatttt gtcattgatt gcaggactta ttcaaccttc tgatggagaa gttttagttt
     2881 ttggaagtcc cgtaacagga acttcttcta aaataggtta tatgcttcaa aaagatcatt
     2941 tgtttgaatg gcgttctatt ctaagtaatg tctatttagg acttgaaatt caaaagaaat
     3001 taacagatga aaacaaagct tatgtaaaca agctattaga tatttatggt ttggctgatt
     3061 ttaaaaaccg ttttccttcg gagctatcag gcgggatgag gcaaagggtg gctcttatca
     3121 gaactcttgc aataagacct gaaatattgt tattggatga acctttttca gcattggatt
     3181 atcagaccag attaattgtt tccgatgaaa ttggcaggat tattaagaaa gaaagtaaaa
     3241 cagccatact cgtgactcat gatattgcag aagctattag tatggcagat aaagttgtag
     3301 tactgactga aaggcctgga aaaatcaaag cgagtcacga tatcaaactt tccattgata
     3361 acaggacacc ttttaaatca agagaggctc cagaatttag agagtatttt aacatcatat
     3421 ggaaggagct gaaaacccat gaaagtgaaa gctattaatt attccaaaga tcatttaaag
     3481 tatttaagcg ctataaaaag acgggaaaaa atcataaaag ccagtcagat atttatttta
     3541 gtatcctttt tattgttgtg ggaaatggct gcaaattttg gctggattga tgcatttata
     3601 tttagtcagc caagtaaaat attaaagact ttatgggaaa tggccttaga tggaagcatt
     3661 tttcttcata caggcataag tctttttgaa acggttgcag gttttttact gagcacttta
     3721 ctggggacat ccatagccgt tttgctatgg tggaataatt ttattttaag agtaacagac
     3781 ccatatctgg ttatcttaaa tagtcttccc aaaacagccc tggcaccgat tcttattgtt
     3841 tggatgggca ataatatgaa atctattctt tttacagcag ttttaatgtc cattgttgta
     3901 actgccttga ctgtattaaa tggttttcag gaagtggatc aggataaaat taaattaatc
     3961 gaaagttttg gaggtaacaa aaaggacgtt ttaataaaag tgatgattcc agcgaatgtt
     4021 ccaactatta ttaatgcatt aaaagttaat gtaggtttgt cgcttgtagg agttatggta
     4081 ggagaatttt tagttgctaa agcaggtcta ggctatctga ttatttatgg aagtcagatt
     4141 tttaaactgg actgggttat gttaagcata gttgttttag cctttttagc ggctttatta
     4201 tataaaatgg ttttaatcct ggaaagacgt tttttaaaat ggagagaata aattctcata
     4261 aaaaatattc acttaaaaaa gtgaatattt ttgtgtaaaa ctttaagaag atttttttag
     4321 attaataaca atgaaataag aggaatggtt atgactgaaa ataaagtaga aataaatacg
     4381 attctcgacg ccaattcact gtttgcgcca tattctgaag ccaggatggc agtattagcg
     4441 gctgctggca tggctccaat aatgacaggc acaccaagca gatatttgtg ataaacaaaa
     4501 ggtgacaaca ttccgtatat tattaaaggg attataatta atcgggtaaa actaatgaca
     4561 taaattcgcc aatttgaaaa gagatacttg ctttcagtat tggccagaag accgccgata
     4621 accagcatag ataagggcgt agtcatggaa cctaagatat caaagcaacc ggttattggg
     4681 gcgggtattg gaattgaaaa aagaaataat ataaaaccaa taatgaccga tatggttccg
     4741 gtattaataa acaatttcca gtcaaatttt ttttggtgtt ttttcccttt tgttagcatc
     4801 caaagaccaa tggtaaaaac gaagacatta aaaggcaagt tataaaaaga gacataaaaa
     4861 agagcttcat cacccaaaac tgcctggaca acaggatagc ccataaagcc tacattggaa
     4921 aatagcaaag tgaattcaaa aatgccagtt tcctcttttt ttgaagcaat taattttggg
     4981 acaagcagtg cgatgataaa agataataga taaataaaaa aagataagag taaaataaga
     5041 ccactctctt ttatactttg ggcagttgct tgctcctggg ttaaagagtt gataattaaa
     5101 gcagggaggg taagctttac taaaatactg ctgattcctt ttatagtaga atcatcaaag
     5161 acgtttcttt tttttgctcc atatccaata accaataata aaaataaaat gagtatttgg
     5221 ttaaatatac tttgaaagtt catgtcaatc cccttttctg tgtgtattaa taagttttct
     5281 tatttcatat tagtatataa taatatagaa gagaagcaaa aatataaaat ttaaacggat
     5341 tatcatataa ttttatatat tgtttaacag ttttctttat ttctttaaaa tttttgttag
     5401 gttgta
//
LOCUS       DAOSUD010000112         5371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_6864_length_5371_cov_28.541949, whole genome shotgun sequence.
ACCESSION   DAOSUD010000112 DAOSUD010000000
VERSION     DAOSUD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6864_length_5371_cov_28.541949"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..2460
                     /locus_tag="PLG49_10195"
     CDS             196..2460
                     /locus_tag="PLG49_10195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17714.1"
                     /translation="MKKKIYLCFSLVLLLLLISYKIYIVCFGLNFQSLNYNNIKKIQE
                     KCKEKDEITFAVIGDIKSSVERFDKKHIGMLNKEEVDFIISTGNALADGSRNKYRILN
                     KSISKLNIPFITAVGEGEVSEDGALRYYKHFGPLYFSFATEDSYFIFMDTTGHTSQAW
                     QKDWLIEELDKASHYKNRFVFMNKPLYKPDNIEASQVKDLCIKDKEFCNFLIKTFSEK
                     NVTGVFSSNIGVYDQKIIEGVPYYITGGAGGPLLLDNPNSYYHYLKVKLSGDNAVVEV
                     KRFDEQTAGGFWESLSSKVFYLYSLFKINFVNILLVINIIYILISLIYIKFTKEVDYY
                     SDFDINIDEGYQNKKLNIAMFTNNYFPFVGGVPISIERLSRGLRKLGHNVYIFAPEYP
                     QGYNDDESVIRCKLITYYETDQFDFPIVNVFLKEIEREFLAKNIDIVHVHHPFGMGHK
                     GVKLGRKHKLPVVFTYHTRFEKYAHNVPIGSEFFKSTIPHKIIKKFAQDCSLIIAPTQ
                     SAKDYLKQIGVIRPVEVMPTGIEFESYEKMDFNKVENIRKVLATEGEVLLISVSRLTK
                     EKNLSFLLDGIKYVRENTKKKFKCLIVGDGPEKQKLQETIDNYNLRDTVLLLGTIDPK
                     EIIYYYEAANIFVFASRSETQGMVLLEAMAGHCPVVAVRSSGTDDMIIEGENGFKTKE
                     DIKLWGEKVIELIENDELRETMSQKAYDFSKGFSIDNMAKKAEKVYYGLLKMQEEMLA
                     FDKNAAKVGGDYHS"
     gene            2645..3127
                     /locus_tag="PLG49_10200"
     CDS             2645..3127
                     /locus_tag="PLG49_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17715.1"
                     /translation="MCVITTENRRGGYISNICKKFGYTPIQVPDESKDGNYFLRIRKI
                     IKDEAQASVAITFDGPLGPLHEPKVFPFAVALFAKRRVVPVSIYVKRKIILKHRWDKF
                     IIPLPFNDINVYVHDPITVDKQDLKDEFEKLRKEAKEMMLEWEKNFQIKKEAKNETTN
                     "
     gene            3111..4163
                     /gene="hydE"
                     /locus_tag="PLG49_10205"
     CDS             3111..4163
                     /gene="hydE"
                     /locus_tag="PLG49_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOQ17716.1"
                     /translation="MKRLIDKLYKQNNLEDKELLELLKGINEEDIVYLKLKALETRKK
                     YYGDKVFLRGLIEFTNYCSRNCSYCGIQAGNKYAKRYRLTLDQILECCETGYILGYRT
                     FVLQGGEDPYYSDEKMVEIIKQIKNKYPDCAITLSLGEKSYESYKKYYEAGADRYLLR
                     HETADKELYEKIHPGASFENRIQCLWNLKEIGYQVGAGFMVGLPNQTYEHYVKDLRFL
                     KELEPHMVGIGPFIPHKDTIFRDEKAGTVEMTTILLALVRLLLPTVLLPSTTALGTID
                     PIGREKGLEAGANVIMPNLSPVDVRDKYALYDGKICTGDEAAECRMCIENRIRKAGFT
                     LDLSRGDHLMFSGAVN"
     gene            4173..>5371
                     /gene="hydF"
                     /locus_tag="PLG49_10210"
     CDS             4173..>5371
                     /gene="hydF"
                     /locus_tag="PLG49_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOQ17717.1"
                     /translation="MNQTPRANRKHIVLYGKTNSGKSSIFNALLGQDVSIVSSVKGTT
                     TDPIIKSMELIPFGPVVVIDTAGLDDKTELGELRIKQSLKMLQRADYALYIMDANDID
                     EMAYKKMADDFKKFNIAHSLVINKVDSITEEKVQALKEKYKEAVFVSVHDEDSITHLK
                     NVLIDKLKSQETEKTILGDLVPYNGTVVLVIPLDSEAPKGRLILPQVQVIRDCLDHGI
                     KSYVVRDTELEEALKDLKQIDLVITDSQIFKKVDSLVPEEIMLTSFSILFARYKGELD
                     VFLEGISAIKNLSENAKILIAESCSHTSSHEDIGKVKIPSMLQKKLGKRLVFEFTAGH
                     DFPEDLKQYDLIIHCGGCMMNEKAMKTRIKICQENHIKITNYGIVLAYLTGILDRSIK
                     IFQKNNL"
BASE COUNT         1921 a          685 c         1060 g         1705 t
ORIGIN      
        1 tttatatatt gtttaacagt tttctttatt tctttaaaat ttttgttagg ttgtaatttt
       61 ggagaataaa tattaaaata aaacttaaag cttatggatt ttctatgctt gtaatgatta
      121 aacgattaga gtatcatatg aaaatagagt aggaaatgaa tagcagggga taatccttat
      181 caggaggctt aagtaatgaa aaaaaagatt tatttatgct ttagtttagt tcttttgtta
      241 ttgctaattt cttataaaat atatattgtt tgtttcggct tgaacttcca atctttaaat
      301 tataataata taaaaaagat acaagaaaag tgtaaagaaa aagacgaaat tacttttgca
      361 gtaataggag acattaagag ttctgtagaa agatttgata agaaacatat tggtatgctt
      421 aataaagaag aggtagattt tattatttca acaggaaatg ctttagcaga tggttccaga
      481 aataaatacc gtattttaaa taaatccatc agcaaattaa atatcccttt tattacagca
      541 gtaggagaag gggaagtctc tgaagatggt gcgttgaggt actataagca ttttgggccc
      601 ctttactttt cttttgctac ggaagacagt tattttattt ttatggatac aacaggccat
      661 acatcacagg catggcaaaa agattggttg attgaagaac ttgataaagc aagccattat
      721 aaaaatcgat ttgttttcat gaataaacct ctttacaaac cagataatat cgaagcttca
      781 caagttaagg atttatgtat taaagacaag gaattttgta attttttaat taaaactttt
      841 tcagaaaaga atgtaacagg cgtattttct tcgaatattg gggtatatga tcaaaaaata
      901 attgaaggcg ttccttatta cattactggc ggcgccggag gtccgctttt attggacaat
      961 ccaaacagtt attatcatta tttaaaggta aagctttctg gtgataatgc tgttgttgaa
     1021 gttaaaaggt tcgatgaaca aaccgccgga ggcttttggg aatctctgtc gagtaaggtt
     1081 ttttatttat attctttatt taagattaat tttgttaata tcctgctggt tatcaatata
     1141 atatatattc ttatttcatt aatttatatc aagtttacaa aagaagtgga ttattatagc
     1201 gactttgaca tcaatataga tgaaggttat caaaataaaa aactgaatat tgcaatgttt
     1261 actaataatt actttccttt tgtaggggga gttcccattt ctattgaaag actttcaaga
     1321 ggacttagaa agttaggtca taatgtatat atttttgctc ccgaataccc ccagggatat
     1381 aacgatgatg agagtgtaat ccggtgtaaa ctgattacat attatgagac ggatcaattt
     1441 gattttccta ttgtaaatgt gttccttaaa gaaattgaaa gagaattcct tgcaaaaaat
     1501 atagatattg ttcatgtaca tcatcctttt ggtatggggc ataaaggggt taaactgggc
     1561 agaaaacata aattgccggt tgtatttacc tatcatacca gatttgaaaa atatgcgcac
     1621 aatgtgccta taggaagtga atttttcaaa agcacgattc ctcataaaat cattaaaaag
     1681 ttcgctcagg attgcagttt gattattgcg cctacgcaaa gtgctaaaga ttatttaaaa
     1741 caaatagggg ttataagacc agttgaagtg atgccaacag gtattgagtt tgaatcatat
     1801 gaaaaaatgg actttaacaa agtagaaaat atacgtaaag tgcttgcaac tgaaggagaa
     1861 gttttattga tttctgtatc aagattaaca aaagagaaga acttatcttt ccttcttgat
     1921 ggaataaaat atgtccgtga aaatacaaag aagaaattta aatgccttat tgttggagat
     1981 ggtcctgaaa agcagaagtt acaagagact attgataatt ataaccttcg tgatacggtt
     2041 ttgttgttag gtaccattga ccctaaagaa ataatttatt attatgaagc tgcgaatatt
     2101 tttgtttttg cttcccgttc cgaaacacaa gggatggtac ttttagaagc gatggcaggt
     2161 cattgtcctg tcgttgctgt tcgatccagt gggaccgatg atatgattat agaaggggaa
     2221 aacggattta aaacaaaaga agatataaaa ctttggggag aaaaagtaat tgaattaatt
     2281 gaaaacgatg agcttagaga gacgatgagt caaaaggctt atgatttttc taaaggattt
     2341 tcaattgaca atatggccaa aaaagctgaa aaggtatatt atggcctttt aaagatgcaa
     2401 gaagaaatgc ttgcctttga taaaaatgca gcaaaagtag gaggagatta ccatagttaa
     2461 tgcttttatt caaaacatcc ttagtttttt ctttacaagc tatatccgtt tagtatataa
     2521 gagtagtaaa ataatcatag aaggtcatgg ggaatttttt aaatcagact atgaagaaaa
     2581 atttgttttt tctttttggc atggttgtag ttatagcttt tatccccttt taagaggaga
     2641 aaagttgtgt gttattacaa ctgaaaacag aagaggcggt tatatatcaa atatatgtaa
     2701 aaaatttggt tatacaccta tccaggttcc tgatgaatca aaagatggaa attatttttt
     2761 gaggattaga aaaataatta aagatgaagc acaggcaagt gtagcaatca cttttgatgg
     2821 tcctttaggt cctttacatg agcctaaagt ttttcctttt gccgtagcgc tttttgcaaa
     2881 aagacgagtc gttcctgtat ccatatatgt gaaaagaaaa atcattttaa aacatagatg
     2941 ggataaattt attattcccc ttccttttaa tgatattaat gtatatgttc atgatccgat
     3001 tactgtagat aagcaagatt taaaagatga gtttgaaaag ctaaggaaag aagcaaaaga
     3061 aatgatgtta gagtgggaaa aaaattttca aattaagaaa gaggctaaaa atgaaacgac
     3121 taattgataa attatacaaa caaaataatc ttgaagataa agagttactt gaacttctta
     3181 aaggaataaa tgaagaagat attgtttatc ttaaactcaa agctttagaa acgagaaaaa
     3241 aatattatgg agataaagtt tttttaagag gattaattga atttacaaat tattgcagca
     3301 gaaattgcag ctactgcgga attcaggctg ggaataaata cgctaaaagg tacagactga
     3361 cccttgatca aattctagaa tgttgtgaga caggctatat tttaggatat aggacttttg
     3421 tacttcaagg tggggaagat ccctattaca gcgatgaaaa aatggttgaa atcattaaac
     3481 aaataaaaaa caaatatcca gactgtgcca ttaccctttc tttaggggaa aaatcctatg
     3541 agtcttataa aaaatattat gaagcaggcg cagaccgcta tcttttaagg catgaaacgg
     3601 ctgataagga attgtacgaa aagatccatc cgggagcatc gtttgaaaac aggatccagt
     3661 gtttgtggaa tttaaaagaa attggctatc aagtcggagc aggttttatg gtggggttgc
     3721 ctaatcagac ttatgagcat tacgttaaag atttaagatt tttaaaagaa ttagagcctc
     3781 acatggttgg cattggtccg tttataccgc ataaagatac gatttttagg gatgaaaagg
     3841 caggtacagt tgaaatgaca acgatattac ttgctctggt aagactttta ctgcctacgg
     3901 tcttgttgcc atctacaact gctttgggaa ccatagatcc tataggaaga gaaaaaggtt
     3961 tagaagctgg ggcaaacgtt attatgccta atttgtctcc tgtcgatgta agagataagt
     4021 atgctcttta tgatggtaaa atctgtacag gggatgaagc agctgaatgc cgaatgtgca
     4081 ttgaaaatag aatcagaaaa gcaggtttta cccttgatct ttccagagga gatcatttaa
     4141 tgtttagcgg ggcagttaat taggaggatt atatgaatca aacaccaaga gcaaacagaa
     4201 aacacattgt tttatatgga aaaaccaatt caggcaagtc ttcgattttt aatgctctcc
     4261 tgggacaaga tgtttctatt gtgtcttctg taaaaggaac cacgacagat ccaattatta
     4321 agtccatgga attaatacct tttggtccgg tggttgtcat cgatactgca ggtcttgatg
     4381 ataagacaga gttgggggag ttaagaataa aacaaagtct taaaatgctt cagagggcag
     4441 attatgccct ttatattatg gatgccaatg atattgatga aatggcctat aaaaaaatgg
     4501 cagatgactt taaaaagttt aatatagctc attctttagt cattaataag gtggattcca
     4561 taactgaaga aaaagtacag gccttaaaag aaaagtataa agaagcagtt tttgtatcgg
     4621 ttcatgatga agactctata actcatttga aaaatgtatt aatagataaa ttaaaaagcc
     4681 aggaaacaga aaaaacgata ctgggcgatt tagtacctta taatggtaca gtcgtcctgg
     4741 ttataccgct tgattctgaa gcgcctaagg gcaggctgat tttacctcag gttcaggtga
     4801 ttagagattg tttagaccat ggaattaaaa gttatgtggt aagagataca gaattagaag
     4861 aagcattaaa ggatttaaaa cagattgact tggttataac ggattcacaa atttttaaga
     4921 aggttgacag cttggtgccc gaagaaatta tgttaacaag tttttcgatt ttatttgcca
     4981 gatataaagg ggaattggat gtttttttag aaggcatttc agcaataaaa aatttaagtg
     5041 agaatgcaaa gatattaatt gctgaaagct gtagtcacac aagttcccat gaggatatag
     5101 gcaaggtaaa aattccttct atgttgcaaa aaaaactggg gaaacgtctg gtgtttgaat
     5161 ttacagcagg acacgatttt ccagaggatt taaagcaata tgatttaatc attcattgtg
     5221 gcggatgtat gatgaatgaa aaagcaatga aaaccagaat taaaatttgt caggaaaatc
     5281 atattaagat tacaaactat ggaattgtat tggcatatct tacagggatt ttagacagga
     5341 gtataaaaat ttttcaaaag aataacttaa a
//
LOCUS       DAOSUD010000113         5261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_7015_length_5261_cov_18.398579, whole genome shotgun sequence.
ACCESSION   DAOSUD010000113 DAOSUD010000000
VERSION     DAOSUD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7015_length_5261_cov_18.398579"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="PLG49_10215"
     CDS             complement(<1..1918)
                     /locus_tag="PLG49_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ17718.1"
                     /translation="MQDVFAELNERQKEAVMSTEGYVRVVAGPGSGKTKTLTARYVYL
                     VKELGINPSNILCVTFTNKAAQEMKKRIRKTIGEGYDTGLITTYHGFCCRVLKEDINR
                     LYYPNNFIILDEEDQKAVIKEIYEELNLDRRDGTYEEILKYISLRKNNDIRYVKLLSD
                     PGNNFDINDAENLYDKIFKIYLKKQQKIYGLDFNDLINFTIVLFIDFPEILKKWQIRL
                     SYIQVDEFQDSTRKEFWLVEQLNKEHGNLFVVGDPDQTIYEWRGANPQLLVDFDKYFP
                     SCKTVILEQNYRSTPEILKASNSLIKKNKIRIEKEMFTLNNSWIKVIHYHGKDEKDEI
                     KWITERIKYLNENLGVPKKNIAILYRAHYVSRYIEQGLIEAGIDYTIYGGIKFFQRKE
                     IKDVLAYLRLIAYGDDFSFSRVVNQPSRGIGKQKINYLREKAEQEGLSLYEALKKYHT
                     DSVFKSTKATEFIETIQYFKINYRSYAVSELLQDVLKRTGYEEYLRKEGDQERLDNIA
                     ELFYSIVSLENNNEEELTLEDYLQQIALYTEADRVDEKDSVKLMTIHVAKGLEFPYVF
                     VCGLSEDIFPSTRSLEERKERALEEERRLAYVAMTRAMKELYLTESEGITRNGLKKYP
                     SRFLFEIEENLLNRI"
     gene            complement(2276..>2929)
                     /locus_tag="PLG49_10220"
     CDS             complement(2276..>2929)
                     /locus_tag="PLG49_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOQ17719.1"
                     /translation="GDLTQPQSDTYGNLALKKAKETGAIFPVIAKWDENQYEYLGKWQ
                     VIDWEYSPLHGFPAHYRFLLRRIEPPETEARKAKEIICSQGNLHPQKVSYVTERILRD
                     TRVIQELKVLYNYTCQVCNIPLNRGIGDPIVEGHHIMPLGKPHNGPDIPENVVILCPN
                     HHALFDMGVITIDVAQRRVIHIDPNDSLNGSVLVLKHTINEEFVNYHNKYIFKGLRW"
     assembly_gap    2930..3029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3211..3864)
                     /locus_tag="PLG49_10225"
     CDS             complement(3211..3864)
                     /locus_tag="PLG49_10225"
                     /inference="COORDINATES: protein motif:HMM:NF036920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaWI family type II restriction enzyme"
                     /protein_id="HOQ17720.1"
                     /translation="MIFTVFYYIIFVAKSKGEEFGKMYKKDENIRMRQSYVSKEGTKY
                     EDFVIQLLQSDNYIRANAVIGKGAKGENTEKILLEEGISVDTELLKIKYNSNIISRSF
                     LLDNDVVVYSKPKRAIVCVISVKKSFRERGGETAYWMVKKKESNKPYKYILVTPDNDN
                     ELFKPHEPQKRNKWRSILTAEMDGVFVVKEGNENIEYEEPFFKVGKRHLIKFVKAQI"
     gene            3907..4881
                     /locus_tag="PLG49_10230"
     CDS             3907..4881
                     /locus_tag="PLG49_10230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ17721.1"
                     /translation="MKYLKKENTLEKRIRLDQKVIHAIESIALKKGESFSLAINKILE
                     EYIKEKKIFCSFDKFYSEHPNLKFDLDNKIKVFENDFITVDLTQWKGKINLIVTSPPY
                     NVGLDYNSHNDSNSYEDYLEFSKKWLTKAYDLLAEDGRMCLNIPLDKNKNGLKSVYAD
                     IVTIAKEVGFKYQSTIIWNEQNISRRTAWGSWLSASAPYVIAPVETIILLYKHQWKRK
                     NKGTSTVEKEQFIEWTNGVWTFNGENRKKIGHPAPFPVELPRRCIKLFSFIEDIVLDP
                     FLGSGSTLIAALEEGRQGIGIEIDSSYVELAIKRIKEYSGKQMKLQIQ"
BASE COUNT         1653 a          962 c          745 g         1801 t
ORIGIN      
        1 caattctatt aagcaaattt tcttctattt caaacaaaaa tcttgaagga tactttttaa
       61 gaccatttcg tgttattccc tctgattcgg ttaagtacaa ttctttcatc gcgcgcgtca
      121 tagcaacgta tgccagacga cgttcttcct caagtgcacg ttctttacgt tcttccaaag
      181 aacgagtgct tgggaaaata tcttcgctta atccacatac aaacacatag ggaaattcca
      241 acccttttgc aacatggata gtcattagtt ttacagaatc cttttcatcc actctgtccg
      301 cttccgtata caaagcaatc tgctgcaaat aatcttccag cgtaagttct tcttcgttat
      361 tattttcaag agaaacaatt gaataaaaca attcggctat attatccagt ctttcctggt
      421 ctccttcttt ccgcaaatat tcttcatagc cggttctttt caacacatcc tgcaacagtt
      481 ccgaaacagc ataagaacga taattaattt taaaatattg tattgtttca ataaactctg
      541 tagcttttgt gcttttaaat accgaatcgg tatggtattt tttcaatgct tcatacaacg
      601 aaagcccttc ttgttcagct ttctctctta aataatttat cttttgcttc ccaatacctc
      661 tgctgggttg atttactact ctagaaaaag aaaaatcgtc tccatacgct attaatctca
      721 aataagccag tacatccttt atttcttttc tctgaaaaaa ttttataccg ccataaattg
      781 tataatcaat accagcttct ataagtccct gctcaatata ccgtgagaca taatgggccc
      841 tgtacaaaat tgctatattc ttcttcggta ctcctagatt ttcgtttaaa tactttatac
      901 gttccgttat ccactttatt tcatcctttt catcctttcc gtgataatgt attaccttta
      961 tccaagaatt attaagtgta aacatctctt tctctattct tatcttgttt ttctttatta
     1021 aagaattact ggctttcaat atttcgggag ttgaacgata gttttgttcc aggataactg
     1081 ttttacatga aggaaaatat ttatcaaaat caacaagtaa ctgcggatta gctcctcgcc
     1141 attcatatat ggtctgatct ggatctccca ccacaaacaa attcccgtgt tctttattca
     1201 actgttctac aagccaaaat tcctttcttg tgctatcttg aaactcatca acctgtatgt
     1261 aactcaagcg tatctgccat tttttcagta tctcagggaa atcaatgaaa agaactattg
     1321 taaaattaat caaatcgtta aaatccagac catatatttt ttgctgtttt tttaagtata
     1381 tcttaaatat tttatcatat agattttccg cgtcatttat atcaaaatta ttgccgggat
     1441 ctgacaataa ttttacgtat cttatgtcat tatttttacg taaagaaata tattttaata
     1501 tttcttcgta agtaccgtcc cgcctgtcca gatttaattc ctcatatatt tcttttataa
     1561 ctgctttttg atcttcttca tccaaaatta taaagttatt gggataatat aaacgattaa
     1621 tgtcttcttt taaaaccctg cagcaaaaac cgtgatatgt tgttatcaaa cccgtatcat
     1681 acccttctcc tattgtcttt cttattctct ttttcatttc ctgtgcagct ttgtttgtaa
     1741 aagttacgca taatatattt gatggattaa tgcccagttc tttaaccaaa tatacatatc
     1801 ttgcggttaa tgttttggtt ttgcctgacc ccggccccgc tacaactctt acgtatcctt
     1861 ctgtagacat aacagcttct ttttgccttt catttaattc ggcaaataca tcttgcatca
     1921 cttacccctt ctctcaagca gttttttaaa gtcaatcttt catcccattt tggttactct
     1981 cctgcccaaa atttcaataa accttccttt tttgtttacg tatttatatt accacaaaaa
     2041 gccattcatg gcaatagacg gacaaaggag ggattgaatg cgaaatattc tctggattca
     2101 cctttccgat cccggtagac cgggttgcac gaggtgaacc gtataagttt aaatccacaa
     2161 ttaacataca aacctatagg cacaaaacca caacaaaaaa gagcggctaa ccgcacatta
     2221 atatcgaccg cctgcgagaa atggtgtttt tgttcttaat caagaataac aaattttacc
     2281 atcttaatcc tttgaaaata tatttattgt gataatttac aaattcttca tttatagtat
     2341 gttttaaaac aaggacactg ccatttaaac tatcatttgg gtctatatga ataacacgtc
     2401 tttgagcaac atctatagtt ataactccca tatcaaatag agcatgatga tttgggcaca
     2461 aaattaccac attctccgga atatctggtc cattatgcgg tttacctaga ggcataatat
     2521 ggtgaccttc tacaatgggg tcaccaatcc ctctgtttaa aggaatattg caaacttgac
     2581 acgtgtagtt gtataaaact ttaagctctt gaataactct ggtatctcta agaatacgtt
     2641 ctgtcacata acttactttt tgagggtgaa gattaccttg tgaacaaata atttctttag
     2701 cttttcttgc ttctgtttct ggtggttcaa ttcttcttag taaaaatcga taatgggctg
     2761 gaaatccatg taaaggcgaa tattcccaat caatgacttg ccacttacct aaatattcat
     2821 attgattttc atcccacttg gctataacag gaaatatagc tccggtttct ttggctttct
     2881 ttaaagcaag attaccatat gtatcagatt gaggttgggt taggtctccn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agtcttcact taaaagttca tcatgatatt
     3061 tccgtttttc tccactaaaa gaagcgcata ttgataaaat ttgccctccc cgctgatgaa
     3121 tacctttttg gttattatga taacgtcgtg cttcttgcca agtgagcaaa atgaatcctc
     3181 tcctcctttt taaagctaga gtaaagtaat ttaaatttga gcttttacga acttaataag
     3241 atgacgtttc cctactttaa aaaatggctc ttcatattct atattctcgt taccttcttt
     3301 aacaacaaac actccatcca tttctgcagt caatatgctc ctccatttat ttcttttttg
     3361 aggttcatga ggtttaaata gttcattatc attatcagga gtgactagaa tatatttgta
     3421 aggtttgtta ctttctttct tttttaccat ccaatatgca gtttctcctc ctctttcgcg
     3481 gaaggacttt tttactgaaa ttacacacac tatagctctt tttggcttac tatacacaac
     3541 aacatcatta tccaacaaaa agctgcgtga tataatattt gaattatatt taatttttaa
     3601 caattcggta tcaactgata ttccttcttc tagtaatatt ttttctgtat tttctccttt
     3661 tgctccctta ccaattacag catttgccct gatatagtta tcactttgaa gaagttgaat
     3721 cacaaaatct tcatacttag ttccttcttt acttacatac gactgtctca ttcttatgtt
     3781 ttcatctttt ttatacattt ttccaaattc ctctcctttt gactttgcaa caaatatgat
     3841 ataataaaat acggtaaaaa tcattgccat actttttatg gctataaaat tgggaggtaa
     3901 ttaaagatga aataccttaa aaaagaaaat actctggaaa aaagaataag attagatcaa
     3961 aaagtgatac atgctataga aagcattgcg ttaaaaaaag gtgaaagctt tagtcttgct
     4021 ataaacaaaa ttttagagga atacataaaa gaaaagaaaa tattttgttc ttttgataag
     4081 ttttatagtg aacatcctaa cttaaagttt gatttggata ataaaataaa agtatttgaa
     4141 aacgatttta taactgtaga tttaacccaa tggaagggaa aaattaacct aatcgtaaca
     4201 tcaccacctt ataatgtggg acttgattat aattcgcaca acgactcaaa tagttatgaa
     4261 gattatcttg aatttagtaa aaaatggctt actaaagctt atgatttact tgcagaagat
     4321 ggaagaatgt gtttgaacat tcctctagat aaaaataaaa atggtctaaa aagcgtttat
     4381 gctgacatag ttacaatcgc taaagaagta ggatttaaat accaaagtac tatcatttgg
     4441 aacgaacaaa atatctctcg taggacagcg tggggttcgt ggttatcagc atctgcacct
     4501 tacgtaattg cccctgttga aaccattata cttctataca aacatcaatg gaaaagaaaa
     4561 aataaaggta catctacagt tgaaaaagaa caatttatag aatggacaaa tggtgtgtgg
     4621 acttttaatg gagaaaatag aaaaaagatc ggacatcctg caccttttcc tgttgaatta
     4681 ccaagaagat gtataaaact tttcagtttt atagaagata ttgtgcttga cccatttttg
     4741 ggaagtggct ctactttgat cgctgccctt gaagaaggta ggcaaggaat agggattgaa
     4801 atagattcct cttacgtaga attagctatc aaacggataa aagaatattc tggaaaacaa
     4861 atgaaattac aaatccagta attaagtaaa tcattctgct aaaaaatgca atttaaatac
     4921 aattaatatt atatatggaa attttgatgg cgtcttattg aaaagcatgt cgcataggga
     4981 ccgtccatgg agagaagcca gaaaagggct agcaccggat caaccttcaa acaaagtaat
     5041 ttcaaagatg agtataaaaa attattttaa agaaataaaa gagatctttg atataaataa
     5101 ttttgctaat ataaaaaaag tataaggctt tatgaggacg ttttgttcgg gtaaatcgga
     5161 atttaacaca aatataattg cttacaagaa gactccttat ttatacactc ccgctccaaa
     5221 tagagctaca gcaagtacag gaacggctta aaaggcttag a
//
LOCUS       DAOSUD010000114         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_7654_length_4847_cov_25.232888, whole genome shotgun sequence.
ACCESSION   DAOSUD010000114 DAOSUD010000000
VERSION     DAOSUD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7654_length_4847_cov_25.232888"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..580)
                     /locus_tag="PLG49_10235"
     CDS             complement(56..580)
                     /locus_tag="PLG49_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17722.1"
                     /translation="MYYNQSFYQADYRMPSNSMNFQALKDFQTHIPQSYRPARLRIDD
                     KISELIEEAAKMERELFSYDKKIMDLLENEADQKKILRISINQRKHEKMFGEIYQGIT
                     GRKLLLDLKDFDIKEEDIRKIIDYRIFKGFDAIDFYRKIYFLLMPLEWKQIMYEILGD
                     EQNDIIKYIYYLNK"
     gene            777..1538
                     /locus_tag="PLG49_10240"
     CDS             777..1538
                     /locus_tag="PLG49_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOQ17723.1"
                     /translation="MKKAFRLLFPVFLVIILYMISLSSVFADIKKHSTFAQATVKQAE
                     ITAFLLNVRTGPDTSYPVISRLRKGQIVNVLGKLNDWYVVHLPDDSVGVISSKYTKPY
                     SYNENVLNNIENNQIVTTSSNDTVSIQEKEKIMLEYINNERKKVGLSPYILDDTLSKI
                     ASIKAKDMAENKYFGHVSPTYGSPFEMMKQFGISYKIAGENIAGNNSVKNAHDSFMNN
                     PGNKANILSKNYEKIGIGIAEDERYGYVYVQIFMK"
     gene            complement(1600..2730)
                     /locus_tag="PLG49_10245"
     CDS             complement(1600..2730)
                     /locus_tag="PLG49_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOQ17724.1"
                     /translation="MKAAIIYGGMKGKDGLLNSIVSIISETLKELHVEVEEILLDNYH
                     IDYYYGKSNKDIQQIANKINDSAGVIFVSNTILAAPSGIIKVFLENLGHLSYKNMIEG
                     KYGFSVCFTDSLGERESAEYILKAWEMLGGIEGGKIALWVKDMEGLKNLRESIEKRVE
                     AFYKIFRQPGQKIPNSYNQSVTMTVNEKKISGWTVPKIDLDEINKQNNQEETVQVKNS
                     SFEDFSKVQEKDIKELTDFFKQQMFSAGNELAINAYGTYAKPSAMPVVEHRKTCKQMT
                     KNLPHYFQPHLAADFVGSFQFNITGKESFDGYIVIDKGECSYFDGIKEDADITMIMEE
                     DIWVDILRGKISAQKAFMTGQLKVRGNFVLLNRLDQLFKKMS"
     gene            complement(2755..4305)
                     /locus_tag="PLG49_10250"
     CDS             complement(2755..4305)
                     /locus_tag="PLG49_10250"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOQ17725.1"
                     /translation="MLTKKWFKTVAMIMLLCLICIACSNDKNKSSDREEEPVVVILDE
                     SSKKEEKVKKETSKDSNTTYKYTDYKVNLKIDPEKRTFEGIEKVNFTNKTEDYFETIL
                     FNLYLNAYQSNSQVKPYTLESEDKIYRDGFDEGYIEIESVLINNTEAAFNQSETFLEL
                     LLQEPVSPEEEIEITIQFKGKVPKASHEIGANDKVMWMGNFLPVLAVYDEDGWSRYSY
                     YPIGDSFYTRISNYSVTVTTPKDYTVVGTGEETEQENEKEKVTTIEAKMVRDFALAVS
                     NQFNVETREVQDGININLYYTSKMGVDGENVLETAEKTLKLYGEILGSYPYTELDIIE
                     GTLYNSSGAEYSALVVADYKVLRGISSQEIIAHLVGHQWFYGVVGSNPVKEPWLDEAI
                     VTYLTNYIFYSDEEIERLMQNKRREINKNIGVLRVTRLNSELRDFSTWESYYNIHYYR
                     GEIMLHALRKRMGDEKFREFLKKYYERYAYKIANKEAFMEVAEEVYDSSLKMFFYQWM
                     DAEKLPKF"
     gene            complement(4394..>4847)
                     /locus_tag="PLG49_10255"
     CDS             complement(4394..>4847)
                     /locus_tag="PLG49_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17726.1"
                     /translation="YKYLRKNYKIKELNLIKSLDRFVEEFVRIINLSRAFSRFLGNPV
                     ISIDPLVVDLRCYKENKLRKSLKERNGVYEQGYFEVVDISPDCSITIKKKPKGRYAKL
                     LLDDDLVFYLKKGDILQLKITRRFFFTYWEIEEIKSCYLKEAKKYLLG"
BASE COUNT         1573 a          924 c          590 g         1760 t
ORIGIN      
        1 tatagcaata ttatagcata ttttttgaaa agtaaaaata aaaaaattgg ctaagttatt
       61 tgttaaggta ataaatatat tttataatat cattttgttc atctcctaaa atttcataca
      121 ttatttgttt ccattcaaga ggcatcagta aaaaataaat cttacgataa aagtcaatag
      181 catcaaagcc tttaaaaatc ctgtaatcga ttatttttct tatatcttcc tcttttatgt
      241 caaaatcctt taaatccaat aatagctttc ttcctgttat gccctgatat atttccccaa
      301 acattttttc atgctttctt tgattaatcg aaatccggag tatctttttt tgatcagctt
      361 cattttctaa taaatccata atttttttat catatgaaaa aagttctctt tccattttag
      421 ctgcttcttc aataagctca gaaattttat catcgattct caatcttgcc ggccggtatg
      481 attgaggaat atgggtctgg aaatctttca aagcttgaaa attcatgcta ttcgaaggca
      541 tccggtaatc tgcctgataa aagctttgat tataatacat actatttccc cctaacatct
      601 ctccttagtt tctctttata tatttattct taaattcatt tttaatgaca acctttttca
      661 taaccgtttt ttattatttc tatcataagc cctcttaaat ttcattttct agcataaaat
      721 gatttagaat aacaaataat gataataata aattaatgag cagaggatgt gattaaatga
      781 aaaaagcgtt ccgtttactc tttcctgttt ttcttgtcat catcctgtat atgatttctc
      841 tttcatccgt ttttgctgat ataaaaaaac acagtacttt tgcccaagcc acagtcaagc
      901 aggcagaaat tactgccttt ttgcttaacg tacggacagg accggatact tcttatcctg
      961 ttatatcccg tttaagaaaa ggacaaatcg taaatgtttt gggcaaatta aatgactggt
     1021 atgtggtaca cttacccgat gattctgtag gcgttattag cagcaaatat acaaaacctt
     1081 atagctacaa tgaaaatgtc ttaaataata tagaaaataa tcagattgta acaaccagtt
     1141 ccaatgatac agtcagtatc caggaaaaag aaaaaattat gttagaatat atcaataatg
     1201 aaagaaaaaa agtgggtctt agtccttata ttctcgatga taccctttct aaaattgctt
     1261 caatcaaagc aaaagatatg gcagaaaata aatactttgg tcatgtttcc ccgacttatg
     1321 gttctccctt tgagatgatg aaacaatttg gaatttctta taaaatcgct ggagaaaaca
     1381 ttgcaggaaa caattcggtc aaaaatgccc atgattcttt tatgaacaat ccaggcaata
     1441 aagcaaatat cttaagtaaa aactatgaaa aaatagggat tggtattgca gaagatgaaa
     1501 gatatggtta tgtgtacgtt caaatattta tgaagtaaaa aggatattgt ataaaataaa
     1561 ccggaacttg tatcttcaat atacaagttc cggtcttttt taagacattt ttttaaataa
     1621 ttgatccaat ctatttaata atacaaaatt acctctgact tttaactgtc cagtcataaa
     1681 agctttttgt gcagaaattt ttccccttaa aatatcaacc caaatatctt cttccataat
     1741 catggtaata tctgcatctt ccttaatacc atcaaaataa gaacattctc ctttatcaat
     1801 cacgatataa ccatcaaaac tttctttacc cgtaatatta aactgaaagc tgccaacaaa
     1861 atctgcagct aaatggggtt gaaagtaatg gggaagattt ttagtcattt gcttacaagt
     1921 ttttctatgt tcaacaactg gcatagctga aggctttgca taggttccat aagcatttat
     1981 agctaattca tttccggcag aaaacatctg ttgtttgaaa aaatccgtta attctttaat
     2041 atctttttct tgcacttttg aaaaatcttc aaaggaactg ttctttacct gaaccgtttc
     2101 ttcctgatta ttctgcttgt ttatttcatc cagatcaatt tttggcacag tccagccaga
     2161 tatctttttc tcattaactg tcatggtcac agactggtta taactattgg gaattttttg
     2221 accaggttgc ctgaatatct tataaaaggc ttcaactctt ttttcaatac tttctctcaa
     2281 gttttttaag ccttccatat ctttaaccca taaagcaatt tttccgcctt ctatgccacc
     2341 taacatttcc catgccttta aaatatattc tgcactttct ctttctccta aactatctgt
     2401 aaaacaaact gaaaatccat atttaccttc tatcatattt ttataagaca agtgacctaa
     2461 attttctaaa aagaccttaa taattcccga aggagcagct aatatggtat tggaaacaaa
     2521 aataacccca gcagaatcat taattttgtt ggcaatttgc tgaatgtctt tattactttt
     2581 tccataataa taatcaatat gataattgtc taataaaatt tcttctacct caacatgcag
     2641 ttcttttaaa gtttctgaaa taatactcac tatagaattt aataaaccat cttttccttt
     2701 catcccacca taaataatag ctgctttcat tctcccaccc cctgcaaaca aggattagaa
     2761 cttaggtaac ttttctgcat ccatccattg ataaaagaac atctttagag aagaatcata
     2821 aacttcttct gctacttcca taaaagcttc tttattagct attttgtagg catatctttc
     2881 ataatatttt ttaagaaatt ccctgaattt ttcatcgccc atcctcttgc gcaatgcatg
     2941 aagcataatt tcgcccctat aataatgaat attataataa gactcccaag tcgaaaaatc
     3001 tcttaattca ctattcaagc gggttactct cagcacaccg atgtttttat taatttctct
     3061 gcgcttattt tgcatgagtc tttctatttc ttcatcagaa taaaaaatat aatttgtaag
     3121 atacgttaca atggcttcat ctaaccacgg ttcttttaca ggattgcttc ctacaacacc
     3181 ataaaaccac tgatgcccaa ctaaatgagc aatgatttcc tgactggaaa tacctctcaa
     3241 taccttgtaa tctgcaacta ctaaggccga atactctgca cctgaagaat tatacaaggt
     3301 tccttcaata atatcaagct ccgtatatgg ataggaacct aaaatctcac catataactt
     3361 taaggttttt tctgctgttt ctaatacatt ttctccatct acacccatct tactggtata
     3421 gtataaattt atatttatac catcctgaac ttcccgggtt tcaacattaa actgattgct
     3481 tacagcaagg gcaaaatccc ggaccatttt tgcttctatc gtagtaactt tttctttttc
     3541 attttcctgt tctgtttcct caccagttcc tacaaccgta taatctttag gagttgtaac
     3601 cgttactgaa taattagata tccttgtata aaacgaatca cctataggat aataagaata
     3661 acggctccag ccatcttcat cataaactgc taatacggga aggaaatttc ccatccacat
     3721 aaccttatca ttagcaccta tctcgtgaga agcttttgga accttacctt taaattgtat
     3781 cgttatttct atttcttcct ccggactgac cggttcttgc aaaagcagtt caagaaaagt
     3841 ctcactttga ttaaaagcag cttctgtatt gttaattaaa acagattcta tttcaatata
     3901 accttcatca aaaccatccc tgtatatctt gtcttcagat tctaaggtat aaggctttac
     3961 ctggctgtta ctctgataag cattaagata aagattaaat aaaatggttt caaaataatc
     4021 ttcggttttg ttagtaaaat taactttttc tatcccttca aaagttcttt tttcaggatc
     4081 tatttttaaa ttaaccttat aatccgtata tttataagtt gtattactat ctttactggt
     4141 ctcttttttg actttttctt ctttttttga agactcgtct aaaataacca caacgggttc
     4201 ttcttctcta tctgaacttt tatttttatc attggaacaa gcaatacata taagacacaa
     4261 tagcataatc attgcaacag tcttaaacca ttttttagtc aacaaataaa caccccccta
     4321 ttttgtcgta aaccactccc agtttattat cttacatttt tttataaaag taaaggaaat
     4381 tgtttttctc accttatccc aaaagatact ttttagcttc ttttaagtaa cagcttttta
     4441 tttcttctat ctcccaataa gtaaaaaaaa atcttctggt tatttttaac tgaagaatat
     4501 cccccttttt aagataaaaa accaggtcat catctaaaag cagcttggca tatctgcctt
     4561 taggtttttt ctttatggtt atagaacaat ccggagaaat atctaccact tcaaaatagc
     4621 cttgctcata aaccccattt ctttctttta aactctttct taacttattt tccttataac
     4681 accttaaatc gactaccaaa ggatcgatgc tgattacagg attgcccaga aatctggaaa
     4741 aagctcttga taaattaata atccgtacaa attcttctac aaatctatct aaggatttaa
     4801 ttaaatttaa ttctttaatt ttataatttt ttcttagata cttataa
//
LOCUS       DAOSUD010000115         4734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_7833_length_4734_cov_22.874332, whole genome shotgun sequence.
ACCESSION   DAOSUD010000115 DAOSUD010000000
VERSION     DAOSUD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_4734_cov_22.874332"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..719
                     /locus_tag="PLG49_10260"
     CDS             477..719
                     /locus_tag="PLG49_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOQ17727.1"
                     /translation="MIEKVMGYFLGYIDKSGLYVIKPRLEHASSFQNGYTLGEWVTAF
                     NSNLTRIIIAEGDKMIFALRNKLYYGYFIILFLSTL"
     gene            complement(680..1315)
                     /locus_tag="PLG49_10265"
     CDS             complement(680..1315)
                     /locus_tag="PLG49_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17728.1"
                     /translation="MDNHLTIELRLNIEDIKEYTFYNIHKHLKAIPALCISAMLHILL
                     GILITASVEVIIFDTILKSPSPSILFDLGIAITLIIGLWPLLLPVYSILVFFIAYLSR
                     KKKMMNHKTTEPLIHINFNKDKIIIKNLDLNTLNHLNWDEVFQIIDFPALIEISFKST
                     SEKKPLSSERITNLKMLLLIPKRCFKNQADLDNFLNLISQRVDKNKIIKYP"
     gene            complement(1399..2187)
                     /locus_tag="PLG49_10270"
     CDS             complement(1399..2187)
                     /locus_tag="PLG49_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOQ17729.1"
                     /translation="MGKNELTISKVMIKILRLFITTIAALIAVLLLINFIPFIKRSSF
                     NIGLTLAVLICIFFFDKEKLHDFGLKISKESLSLFIKGLAYALISFLISLIAVYFYCL
                     FFEGTKLDILPNRSNISSLSLLSFYVLVAFSEELYFRGYILSNIKRAGSNIPAVIISS
                     FFFMIMHLVNPQASLTMLSGTFVISIYLSVLYLKTNSLWSCIGFHFAWNYLQDSVMLT
                     PTEGPEWIAVAVFTLILFYLCFYYKFLKSVNRKQGNSPEESATN"
     gene            2456..3184
                     /locus_tag="PLG49_10275"
     CDS             2456..3184
                     /locus_tag="PLG49_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ17730.1"
                     /translation="MNFKNKIVIVSGTSSGIGKACAELLLEKEAMVVGLDIKEPSISH
                     ARYIHYLMDVSDEKEVMGLIKDIDEEFGKADGLVNCAGIFINAKPFYELDLSEWNKVI
                     DTNLTGIFILSKHIAKIMMKHNKGKIVNISCIRSKIFRPKMAEYAASKAAVVALTSAM
                     ALDLAPYNIQVNSVAPGFTYTAMTAKSFDDPKIREFSESIIPAGRIAKAEDIANVVIF
                     LLSEMSDYINGETIFVDGAYRISK"
     gene            3205..3648
                     /locus_tag="PLG49_10280"
     CDS             3205..3648
                     /locus_tag="PLG49_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ17731.1"
                     /translation="MTDMNPSKELRERIRKLEKELGVLKDSQLSCCGVTLAQCHALVE
                     IGRAGRISLSELARLLNLENSSMSRTVDKLVTKGMVKRDIDPVDRRYLVINLTARGEE
                     VYRKIEENMNLFFEKIYLALPAEKRNDVLESLNVLLASVEKVRDE"
     gene            3973..4665
                     /locus_tag="PLG49_10285"
     CDS             3973..4665
                     /locus_tag="PLG49_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17732.1"
                     /translation="MKKMSVLLAIIILLFGVTACGTGKESASSGNLTSEVSNNATKTL
                     EENQEEPDSSADEKEEKKEDSSVELPYTIMFPMHSLYVKAPNSYLIEYGATTFIVVEG
                     YTLIRLTSDSFVDASTINEPSEILDAYFDSFTYNANQFGSGDPDRFDIRESREVEIDG
                     RKFWRFEGDLINKLPSGDEIRYFMVGYTFFWDDTPFQITATVFTEEPEQAEIDKARAY
                     VDEMVKTVRDKR"
BASE COUNT         1732 a          571 c          881 g         1550 t
ORIGIN      
        1 cacaatcaca ggcagaaatg gctgaaaaac taatgcatct ggtagagaaa tttagagttt
       61 aggcagagga attcattttt taaatataaa tatttaaaga tttaaaccct tgatatggct
      121 tagcctatca agggtctttt atttaaagaa aatatttagc agggatttat ggtttttgac
      181 tactaaaaag ttaaaaaaga aatttgaaaa aatttatggg gtatctcaag taaaaattct
      241 tgaaaaaatt tttagttaaa atcagactta aatggtaaat gaaataactg tacaatgtac
      301 gctaaatatt ctgtatatta taattgcttt aaaataatac aatagattat gacaagttta
      361 acagtaagca aagaacaatg ctgttaattt ttattgcatc aatcattttt cataattatt
      421 gatacaaaat taaatggtag catccgtata gttatttaaa gattataagg agtgagatga
      481 ttgaaaaggt catgggctat tttttaggtt atattgataa aagtggtctt tatgttataa
      541 agcctcggct tgaacatgca tcgtcattcc aaaatggtta taccttagga gaatgggtaa
      601 ctgcttttaa cagtaatttg accaggatta tcattgcaga aggagataaa atgatttttg
      661 cattgaggaa caaattgtat tatggatatt ttataatttt atttttatca actctttgag
      721 agataagatt tagaaagtta tctaaatctg cttgattttt aaaacatctt tttggaataa
      781 gtaataacat ttttaaatta gtaattcttt cagacgataa aggttttttt tcggatgttg
      841 atttaaaact aatttcaatt aaagcaggga aatctatgat ctgaaaaacc tcgtcccaat
      901 tgagatgatt aagggtattt aagtcaaggt ttttaataat gattttatcc ttattaaaat
      961 ttatatggat taatggctct gttgttttat gattcatcat tttcttcttg cgagataaat
     1021 aagcaataaa aaatacaaga atactgtaca ctggaagaag taatggccaa agtccgataa
     1081 ttaatgttat agcgattcct aagtcaaaga gaatagatgg tgatgggctt tttaaaatag
     1141 tatcaaaaat gattacttca acagaagcag ttatcaatat gcccagaagt atgtgtaaca
     1201 tagcactgat gcaaagggca gggattgctt ttaagtgttt atgtatgtta taaaaggtat
     1261 attctttaat atcctctata ttaaggcgta gttcaatggt taaatggtta tccatatttt
     1321 ttctcctaaa tatacatact attaatttta ttataaataa ttttttgctt atctcaagta
     1381 agaatatcta ataaggcatt aatttgttgc agactcctct ggcgaatttc cttgtttgcg
     1441 attgacagat tttaaaaatt tataataaaa gcaaaggtag aaaagaatta aagtaaaaac
     1501 tgctacagct atccattcag gtccttctgt tggtgtgagc atgaccgaat cctgcaggta
     1561 attccatgca aaatgaaatc caatgcagct ccacaggctg tttgttttaa gatacaggac
     1621 ggataaatat atgcttatga caaaggtgcc tgagagcatc gtcaggcttg cctgaggatt
     1681 aaccaggtgc ataatcatga aaaagaatga tgaaataatg acagccggga tatttgaacc
     1741 tgctcttttt atatttgaga gaatataacc cctaaagtat aattcttcac taaaagcgac
     1801 taagacatag aaactcagta aagataaact gcttatattg gacctgttcg gtaatatatc
     1861 aagctttgtg ccctcaaaaa ataaacaata gaaatatact gcgattaaac tgataagaaa
     1921 agatattaga gcatatgcta aacctttaat gaataaagat aagctttctt ttgaaatttt
     1981 aagaccaaaa tcgtggagtt tttctttatc aaagaagaaa atgcatatta aaacagccag
     2041 agtaagacct atattgaaag atgatctttt tataaaagga ataaagttta ttaaaagcag
     2101 aacggctatt aaggcagcaa tcgtcgttat aaataatcgc aaaattttaa tcattacttt
     2161 tgagatggtt aattcatttt ttcccatttt tttctccttt caaaagaata aatgatgata
     2221 cgataagaga attaggattg ttatgaatta ttgaaatttt atattaacat aaaaatttac
     2281 gatagatttt agtccaggtc tgaaaaatga ttcattcaat ttaatacagg gaaaaattgt
     2341 taggctggta ttggagattt agtcataata attttatcta aattttttaa aatagttgca
     2401 tattctaata tttgtatgat acaattatta aaaattttat attggaggaa gaagaatgaa
     2461 ttttaaaaat aagattgtta ttgtttcagg gaccagttca ggcattggta aagcttgtgc
     2521 agaactgctt cttgaaaaag aagcaatggt tgtaggttta gacataaaag agccttcaat
     2581 aagtcatgca agatatatac attatttaat ggatgtcagt gatgaaaaag aagtcatggg
     2641 attaataaag gatattgatg aagagtttgg taaagcagat ggcctggtta attgtgccgg
     2701 catttttata aatgctaagc ccttttatga attggatttg tctgagtgga ataaagtgat
     2761 agatacaaat ttaacaggga tattcattct ttccaagcat atagcaaaaa tcatgatgaa
     2821 acacaataaa gggaaaatcg tcaatatcag ctgcataaga tctaaaattt tcagacctaa
     2881 aatggcggaa tatgctgcgt caaaagctgc agtcgttgca ttgacatcag caatggcttt
     2941 ggatttagca ccttataata tacaagtcaa ttctgttgcc cctggcttta cttatacagc
     3001 tatgacagca aaatcatttg atgatccaaa aataagggaa ttctctgaaa gcatcattcc
     3061 agccggaaga attgcaaaag ctgaagatat agccaatgtt gttatatttt tattatcgga
     3121 gatgtcagat tatattaatg gtgaaacgat atttgtggat ggtgcttata gaatatctaa
     3181 ataaaaaaga tatgggggaa gtatatgacc gatatgaatc cttcaaaaga attaagagaa
     3241 agaatacgaa aacttgaaaa agaacttggc gttttaaaag attctcagct ttcttgctgt
     3301 ggtgtgactc tggcccaatg ccatgcatta gtagaaatag gacgggcagg aaggatttct
     3361 ttgagtgaac tggccaggct ccttaatctt gaaaacagct caatgagccg gacagttgat
     3421 aaactggtaa caaaaggtat ggtgaagcgg gatattgatc cagtggacag gagatatctt
     3481 gttataaatc ttacagcaag aggagaagaa gtttatagaa agattgaaga aaacatgaat
     3541 ttattctttg aaaaaattta tctggcttta cctgctgaaa aaagaaacga tgtattagaa
     3601 agtctaaatg ttttactggc gtctgttgaa aaagtaagag atgagtaaga taaagggact
     3661 gaaaatttga ggatgtgtat aaaaacaatt taaagactgt tattcaacat ttaaatgttg
     3721 tttgacagtc ttttatgtta ctattgtctg ataataatat atggttttga taatttccta
     3781 tattgacaag aattataaaa catatttcaa tgattggtgt tttttaaatg tttttatcaa
     3841 aaaatatata aaattaaagg gtattcacgt tgatatgaat attacttttt ttgatagcta
     3901 tgtgtttgtt tagtattaaa tgaaattaag tatataaaaa ataataaata attatggaag
     3961 gggctaaaga caatgaagaa aatgagtgtt ttgcttgcaa tcattatttt actttttgga
     4021 gtgaccgctt gtgggactgg gaaagaaagt gcaagctcag gtaatctgac cagtgaagta
     4081 agtaataatg caacaaaaac attagaagag aaccaagaag agccagatag cagtgcagat
     4141 gaaaaggaag aaaaaaaaga agacagttca gttgaactac catatactat aatgtttccc
     4201 atgcatagct tgtatgtgaa agcacctaat tcatatctga ttgaatatgg ggctacaact
     4261 tttattgttg ttgaaggata tacacttatt agattaacat ctgattcatt tgtagatgca
     4321 agtacaatta atgaacccag tgaaattctg gatgcatatt ttgattcttt tacatataat
     4381 gcgaaccaat ttggttcagg tgatccagac aggtttgata taagggagag cagagaagtt
     4441 gaaatagatg gtagaaaatt ctggagattt gaaggagatc ttataaataa actaccttct
     4501 ggtgacgaaa taaggtattt tatggttgga tatacgtttt tctgggatga tactccattt
     4561 cagattactg ctacagtatt tactgaagaa cctgaacaag cggaaataga taaagcaagg
     4621 gcatatgttg atgagatggt taagacagta agagataaac gctagtaaaa tgtgttacta
     4681 aaaggctgtt attcaacttt tgttgagtaa cagccttttt ataaaaaagc ctat
//
LOCUS       DAOSUD010000116         4650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_7976_length_4650_cov_22.003264, whole genome shotgun sequence.
ACCESSION   DAOSUD010000116 DAOSUD010000000
VERSION     DAOSUD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7976_length_4650_cov_22.003264"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLG49_10290"
     CDS             complement(<1..800)
                     /locus_tag="PLG49_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17733.1"
                     /translation="MSIIGGELFFINQNYKYVDNSLFDFLNNFNTNLYLYSSGRDALY
                     SIFNHIKDKKIWVPNFLCESIFKPIIKNNLRYEFYNVNDRLEAILQFKPAKDDYILII
                     NFFGFVDENLYKFSVDNKLNVISDTTHLIFNEERFKNVLSKSKFVVGSLRKSFALPDG
                     GYVLSNDHLVIKNKPREEFTYLRTYGLFSRYFSSINNFNSDENFIVLKKAESVLDKAT
                     DFGYKISYLSENILKCINLNDYIDQINKNFNILNKKLNKNYIFYSQYFP"
     gene            complement(797..1789)
                     /locus_tag="PLG49_10295"
     CDS             complement(797..1789)
                     /locus_tag="PLG49_10295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17734.1"
                     /translation="MNVSFTDKIGNLRNSLYFSKSYNEIFAKYDNSHFVIMKLNKNNK
                     ESYLPLLLHEINDYYEGYSSYGYGGIVGNIDHLSKDDFRKIIEFCKSNKIIDIFIRNA
                     PFLNNHKIIPDEFNFFNRVTYIKELKLYKSFDEFTRVLNQKLRWSINAALKNNLEVNI
                     KKYSEITDKDLDNFYSLYNKVMNIRKASEYYYFPKEFFQNTFNSLKEKIELFYIAVDG
                     RFIAASMFMLDDDYVHYHFSASDRDFSKLQPMELLLSKAIYVYGAKGYKYLHLGGGLS
                     PDASDGLSRFKKKFADFESEFYISKIICDKDIYDYLRNYYNVKNNLFLIKDAKL"
     gene            complement(1792..2895)
                     /locus_tag="PLG49_10300"
     CDS             complement(1792..2895)
                     /locus_tag="PLG49_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOQ17735.1"
                     /translation="MVFIKPILLDAPNLGELEKKYLCDCIDSTFVSSVGENIRIFENK
                     FAEYLRAKKTVSTQSGTAAIHLALYELGIGPGDEVIVPVLTFVATVNPIKYVGATPVF
                     VDVDPETWNMDPNEVEKHITNKTKAIIPVHLYGNPCDMDKIMEISKKYNIPVIEDATE
                     SLGATYKDKFTGTFGEINAFSFNGNKIMTTGGGGMIVTNDEEKADHLKFLVNQARDES
                     KGYYHPEIGFNYRMTNLEASLGLAQFQRLPDFLNKKRKFAEIYKQGFENNEKITFQKS
                     YEGAVSSYWLPSIKVESHKTISEIQKELKEKGIPTRRIFVPIVEFPPYREYKKEQYKN
                     AYEIYEKGLNLPGSTMNDFEQIEYIINMLKEVI"
     gene            complement(2901..3872)
                     /locus_tag="PLG49_10305"
     CDS             complement(2901..3872)
                     /locus_tag="PLG49_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOQ17736.1"
                     /translation="MKVLVTGSEGFIGSHLTELLVEKGFEVKGFVRYNFKNDWGWLED
                     SKYKNEIEVYSGDIRDYDSVHDAMKDVDVVFHLAALIGIPYSYISPLAYIKTNTEGTY
                     NVLEACKKLNIERIIHTSTSEIYGTAQYVPIDEKHPYNPQSPYAASKAGADHLALSYY
                     RSFGTPVTIIRPFNTFGPRQSARAVIPTIISQILAGKERIKLGNLSPTRDLNYVKDIA
                     NGFITVGLHEKTIGDVFNLGTGEEISIGNLAQKIIELIGKNVEIIEDTQRIRPEKSEV
                     ERLLSNPEKAKKIAGWEPKYSLEEGLKETIEWIQENLQYYKTDIYNI"
     gene            complement(3877..>4650)
                     /locus_tag="PLG49_10310"
     CDS             complement(3877..>4650)
                     /locus_tag="PLG49_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17737.1"
                     /translation="EFQHGVVSKTHPAYNYGEAILNSEEYKKYTPDYFLTYGEYWNNQ
                     INIPGKAYVVGNPHFHESIKRYKDIEEEKGIILIVSQWTLTEEFVEIAKFLANNLKDK
                     KIVFKMHPGEMRNYNLIKPLEAYRNIEIQKDGDIYELLAKYESIVGCYSTTVFEALAF
                     KKRIYLLDNSYSKNNIPEEIGIRFKNFPELKNLIKNKVKNENEPDIEYYFNSNWQENY
                     KKFIEQEIGITINNKNEKIFFKISQQKQKDSKYFEQTED"
BASE COUNT         1432 a          790 c          579 g         1849 t
ORIGIN      
        1 gggaaatatt gtgaataaaa aatataattt ttatttaact tcttatttaa tatattaaaa
       61 tttttattta tttgatctat ataatcgttt aaatttatac attttaagat attttctgat
      121 aaatagctaa ttttataacc aaaatcagtt gccttatcca aaaccgattc agcttttttt
      181 agaacgataa aattttcatc gctattaaaa ttgttaatac tactgaagta tctagaaaaa
      241 agcccatatg ttcgcaaata cgtgaactct tctctcggtt tatttttaat aactaaatga
      301 tcattcgata gaacataccc accatcaggc aaagcaaaac tctttcttaa actccctact
      361 acaaattttg acttagatag tacatttttg aatcgttctt cgttaaatat taaatgtgtt
      421 gtgtctgaaa taacatttaa tttattatca acactaaatt tatataaatt ttcatcaaca
      481 aaaccgaaga aattgataat taaaatgtaa tcatcttttg caggtttaaa ttgtaggatg
      541 gcttctaatc tatcattaac attataaaat tcatatctta aattattttt aattattggt
      601 ttaaaaatag attcgcacaa aaaattaggt acccatattt ttttatcttt tatatgatta
      661 aatatagaat ataatgcatc tcttccagat gaatacaagt acaaattagt attgaaatta
      721 tttaaaaaat cgaataaact attatcaaca tatttataat tttgatttat aaaaaataac
      781 tctccaccta ttatactcat aatttagcat cctttattaa aaacaaatta tttttcacgt
      841 tgtaatagtt tcgtaaataa tcatatatgt ccttgtcaca gattattttt gaaatataaa
      901 actctgattc gaaatctgcg aatttctttt tgaatcttga aagaccgtca ctggcatctg
      961 gagataatcc cccacctaaa tgcaaatatt tatacccttt tgccccatac acataaatag
     1021 cttttgacaa taatagttcc ataggctgga gttttgagaa atctcggtct gaagcactaa
     1081 aatgataatg aacatagtca tcatcaagca tgaacattga agcagctata aacctaccat
     1141 caacggcaat ataaaataat tcaatttttt ctttcaatga gttaaatgta ttttgaaaaa
     1201 attcttttgg gaagtaataa tattcgctcg cttttctaat attcataact ttgttataga
     1261 gactataaaa attgtccaaa tctttatctg ttatctcgct atactttttt atattaactt
     1321 ccaaattatt ctttagtgca gcattaatag accatctcag tttttgattt aaaactcttg
     1381 taaattcatc aaaagattta tataacttta attcttttat ataagtgact ctgttaaaaa
     1441 aattaaattc atctgggatg atcttatgat tatttaaaaa aggggcattc cttataaaaa
     1501 tatcgattat tttattactt ttacaaaatt caattatttt tctaaaatca tctttgctta
     1561 aatggtcgat atttcctact attcctccat aaccatagct actgtaacct tcataataat
     1621 cgtttatttc atgcaatagc aaagggaggt aactttcttt gttgttttta tttaatttca
     1681 taatgacaaa atgactgtta tcgtatttgg caaaaatttc attataactt ttactaaaat
     1741 aaagagaatt tctcaaattt ccaattttat cagtaaaaga tacattcact tctatatgac
     1801 ctcttttaac atattaatta tatattcaat ctgttcaaaa tcattcattg ttgaacctgg
     1861 taaatttagt cctttttcat atatttcata ggcatttttg tattgttctt tcttatactc
     1921 cctatacggg ggaaattcta caattggcac aaagattctt cgtgtgggta tccctttttc
     1981 ttttagttct ttttgaattt ctgatatagt tttatgggat tcaactttta ttgaaggtaa
     2041 ccaatatgaa cttacagcac cttcataaga cttttgaaaa gttatttttt cgttgttttc
     2101 aaaaccttgc ttgtatattt ctgcaaactt tctttttttg tttaggaagt ctggcaatct
     2161 ttgaaattga gctaagccta agcttgcttc taagtttgtc attctgtaat taaatcctat
     2221 ttctggatga taatagcctt tgctttcgtc tcgagcttgg ttgactaaaa acttaagatg
     2281 atcagccttt tcttcgtcgt ttgttacaat catgcctcca ccgccagtcg tcatgatctt
     2341 atttccgttg aaactaaatg catttatttc cccaaaggtc ccagtgaatt tatccttata
     2401 tgttgcccct aaactctctg ttgcatcttc gataactggt atattatatt tttttgatat
     2461 ttccattatt ttgtccatat cgcaggggtt accgtataga tgaacgggta ttatagcctt
     2521 tgttttgttt gttatatgct tttccacttc atttggatcc atattccaag tttcaggatc
     2581 aacatctaca aaaactggtg ttgccccaac gtattttata gggtttactg ttgctacaaa
     2641 cgtaagtacg ggtacgatga cttcatcacc tggaccaatt cctaattcat acaaagcaag
     2701 gtgaatcgca gcggttccac tttgagttga taccgttttt tttgctctta aatattcagc
     2761 gaatttattt tcaaatattc taatgttttc cccaacagag gaaacaaaag tagagtcaat
     2821 acaatcgcac aaatactttt tttctaattc ccctaaattt ggtgcatcaa gtaatatggg
     2881 ttttataaaa accacctcta ttaaatatta tatatgtcag ttttgtaata ctgtagattt
     2941 tcttgaatcc attctattgt ttcttttaat ccttcttcta aagaatactt tggttcccat
     3001 cctgcaattt tttttgcttt ttctgggttg gataataacc tctctacttc agatttttct
     3061 ggtcttattc tttgagtatc ttcgattatt tcaacatttt ttccaattaa ttctatgatt
     3121 ttttgggcta aattacctat tgatatttct tcccctgttc caagattaaa tacatctcct
     3181 attgtttttt cgtgtaaacc aacggttata aacccatttg caatgtcttt tacataattt
     3241 aggtcccttg taggggataa attaccaagt tttattcgtt ctttacctgc caagatttga
     3301 cttattatgg ttggaataac ggctctcgcg gactgcctgg ggccaaatgt gttgaatggc
     3361 cttataatag taacaggtgt tccgaaggat ctgtagtaag ataatgccaa atgatctgct
     3421 ccagctttac tggctgcata cggcgattgt ggattatacg ggtgtttttc atctatcggc
     3481 acgtattgtg ctgtcccgta tatttcactc gttgatgtgt ggatgattct ttctatattc
     3541 aattttttac atgcttctaa tacattgtat gttccttctg tgtttgtctt tatgtaagca
     3601 agtggagaga tgtatgaata aggaattccg attaaagcag ccaaatgaaa tacaacatca
     3661 acatctttca tagcatcatg cacagaatca taatccctta tgtctccact gtagacttct
     3721 atctcatttt tatatttaga atcttcaagc cagccccaat cgtttttaaa attataccgc
     3781 acaaatcctt taacttcaaa gcccttttca actaagagtt cggttaagtg tgaaccgata
     3841 aatccttctg accctgtaac tagtactttc attaattcaa tcctccgttt gttcaaaata
     3901 tttactatct ttttgtttct gttgactaat tttaaaaaag attttttcat ttttattatt
     3961 gatagtaata cctatttctt gctctataaa cttcttataa ttttcttgcc aattactatt
     4021 gaaataatat tcaatatccg gttcgttttc atttttaacc ttatttttta ttaaattctt
     4081 cagctcagga aaatttttaa atcttattcc aatttcttca ggtatattat ttttggaata
     4141 actgttatcc aataggtata ttcttttctt aaatgccaaa gcttcaaaaa ctgtcgtaga
     4201 ataacaccca actatacttt cgtattttgc taatagttcg taaatatcgc catctttttg
     4261 aatttcaata tttctatatg cttctagagg ttttatcaga ttataatttc tcatttctcc
     4321 aggatgcatt ttaaaaacta tttttttatc ttttaaatta ttcgctaaaa attttgcaat
     4381 ctcaacaaat tcttccgtta aagtccattg agacacaatc aaaataatac ctttctcttc
     4441 ttcaatatcc ttgtatctct ttatactttc atgaaaatgt ggattaccaa caacatatgc
     4501 ttttcctgga atgttgattt gattattcca atattctcca tatgttaaaa aataatccgg
     4561 ggtatatttt ttatattctt ctgaattcaa tatcgcttct ccataattat aagcaggatg
     4621 cgttttcgaa acaacaccat gttgaaattc
//
LOCUS       DAOSUD010000117         4628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8024_length_4628_cov_21.227640, whole genome shotgun sequence.
ACCESSION   DAOSUD010000117 DAOSUD010000000
VERSION     DAOSUD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_4628_cov_21.227640"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..597)
                     /locus_tag="PLG49_10315"
     CDS             complement(31..597)
                     /locus_tag="PLG49_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ17738.1"
                     /translation="MNKLTDQLKKEKVLVKFLINSAGFGKIGTFSELPLEEQMAMIDV
                     NIKALTTVTYVVLPYLAKGAKIIQVASSAAFLPQPGFNVYAATKAYVHHFSKALSIEL
                     KHRNIQVLALCPGPVKTEFFTIASKDGKPADWKTKFMVNPRDVVSKALKDAESNKMES
                     VYSLPMKLLKIISRIIPQELILKYFKWR"
     gene            complement(627..791)
                     /locus_tag="PLG49_10320"
     CDS             complement(627..791)
                     /locus_tag="PLG49_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17739.1"
                     /translation="MKKIAIVTGASSGLGSHFVRQLDLKYDLDEIWMLARREDKMKEI
                     ASKLKKPKPF"
     gene            complement(852..1169)
                     /locus_tag="PLG49_10325"
     CDS             complement(852..1169)
                     /locus_tag="PLG49_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17740.1"
                     /translation="MELIQSIFWILALWWLLSNVVTAIKVIQIQKAIEKNNELNRNQP
                     KNIELDTKADDPAEVEMVEDKVCGKVIEKSRAYQLFHEGKLFYFCSWDCRQTYIQEYI
                     KQS"
     gene            complement(1198..1371)
                     /locus_tag="PLG49_10330"
     CDS             complement(1198..1371)
                     /locus_tag="PLG49_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17741.1"
                     /translation="MSPMKCPVCHQDNLGLVGRIRYYCPDCNVEIVVKGRVLEIYEIK
                     KDGGLKLVKLLAS"
     gene            complement(1462..1728)
                     /locus_tag="PLG49_10335"
     CDS             complement(1462..1728)
                     /locus_tag="PLG49_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOQ17742.1"
                     /translation="MKESDKKEVINLLKTAKGQLDAIIRMTEEDRYCIDISKQILAVQ
                     ALLKKSNLTVLRQHIHGCVRNALLTGEGEQKIEEIINILDKYIK"
     gene            complement(1798..3069)
                     /locus_tag="PLG49_10340"
     CDS             complement(1798..3069)
                     /locus_tag="PLG49_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOQ17743.1"
                     /translation="MKDWKIETIAVQGGYTPQNSEPRILPIYQSTTYKYDSAEHVAKL
                     FDLEEEGHMYTRISNPTLAALEEKVAQLEGGVGALSTASGQAATTLAILNICQSGEHI
                     VAASNLYGGTFTLLASTFKKFGIEVSFVDPDSSAEEIKKHFRPNTKALFGETIGNPSL
                     NILDFEKFSSIAKEMGVPFIVDNTFATPYLCKPLEHGANVVVHSATKYFDGHATSVGG
                     IIVDGGNFDWTNGKYPGFTEADPTYHGVKYVEQFGKQAYIVKARVQLLRDLGSTLSPF
                     NAFLINLGLETLHLRMERHSENALKLAQFLQGHPKVEWVNYPGLESHRSYALAKKYLP
                     RGCSGVLTFGVKGGKEAGMKFINRVKLAALVVHVADARTSVIHPASTTHRQLTKEEQI
                     SSGVTEDLIRVSVGIENIEDIISDFDQALNS"
     gene            complement(3209..4477)
                     /locus_tag="PLG49_10345"
     CDS             complement(3209..4477)
                     /locus_tag="PLG49_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOQ17744.1"
                     /translation="MRILRKGMRGSDVAELQSILQLIGYRPGAIDGIFGNQTEEAVKA
                     FQRNNALTADGIVGPATYAKLEPILLGYDLYTIKKGDTLFNIAQQYHTTVYAITTANP
                     DINPFFLNVGEEIIVPYGIDLVQTNIPYTYDVMEKDIRGLQMRYPFLEVSTIGQSAEG
                     RNLYLIKLGSGPREVFYNASHHANEWITSPVLMKWVENFSKAFARDGTMRGYPARTIW
                     EQATIYIVPMVNPDGVELVINGVSAENTRYRQLVEWNNGNTDFSNWKANIRGVDLNRN
                     YDASWEEYKEVESLLGIDGPGPYLYGGPYPESEPESKAVADFTRSRNFRLVLAYHTQG
                     EVIFWNYRNLQPPEALRIGEIFASVSGYSLDEPVGPALYAGYKDWFIKEYRKPGYTIE
                     VGRGVNPLPISQFDKIYEDNEELLLLAALV"
BASE COUNT         1397 a          934 c          675 g         1622 t
ORIGIN      
        1 ttctaaatta ttgtttataa taattatatc ttaacgccat ttaaaatatt ttaaaataag
       61 ctcctgaggg ataattcttg aaataatttt taaaagtttc atcggtaaac tatatacaga
      121 ttccatttta ttagactcag catcctttaa agcttttgat acaacatccc tgggattaac
      181 cataaatttt gttttccagt cagcgggctt tccgtctttt gaagcaattg taaaaaattc
      241 tgtttttact ggtcctggac acagggctaa tacttggatg tttctatgtt ttaattcaat
      301 gctcagtgct ttactaaaat gatgtacata agcttttgta gcagcatata cgttaaaacc
      361 cggttgaggt aaaaaagccg ctgaagaggc cacttgtata atctttgccc cttttgccag
      421 gtatggcaaa acaacataag ttacagttgt taaagccttt atattaacgt ctatcatggc
      481 catttgttct tccagtggta attctgaaaa tgtccctatt tttccaaaac ccgcactatt
      541 aattaaaaat ttaactaata ctttttcttt ttttaattga tccgttaatt tattaatttc
      601 ttcaagattg gataaatcac attttattaa aacggctttg gtttttttag cttactggca
      661 atttctttca ttttatcttc tcttcttgcc agcatccaaa tttcatctaa atcatatttt
      721 aaatccaatt gtcgtacaaa atgactgccc aatccagaag atgccccggt tacaattgca
      781 atttttttca tatggacctc catgtacttt tatactttta gtatattatg ttcaaaaagc
      841 agtcctgatt attaggactg cttgatatac tcctgaatat atgtttgccg gcaatcccag
      901 ctgcaaaaat aaaacagctt accttcatga aagagctgat aagcccggct tttttcaata
      961 actttaccac aaaccttatc ttctaccatt tcaacttctg ctggatcatc tgctttcgta
     1021 tccagctcta tattttttgg ctgattccta tttaattcat tattcttttc tattgctttt
     1081 tggatttgaa tgactttgat tgccgtgaca acattagata agagccacca taaagcaaga
     1141 atccaaaaaa tgctttgaat aagttccata ataacaaaat atctcctttc atttccatta
     1201 ggatgctagt agctttacta actttaaacc tccatctttc ttaatttcat atatttctaa
     1261 taccctaccc ttaacaacaa tttcaacatt acaatcggga caataatatc tgatacggcc
     1321 tactaaacct aaattatctt gatggcatac aggacacttc attgggctca catcctattc
     1381 cttctataat acaataaatc atacatatat attataatat ataaatatta aataattgtg
     1441 aacaaaattt aaacaagcgt attattttat atatttatca agaatattga tgatttcttc
     1501 tattttctgt tcaccttcac cagttaataa tgcatttctt acacagccat gaatatgctg
     1561 ccttaataca gtaagattac tttttttaag taatgcctgt acagctagta tttgcttcga
     1621 gatatcaatg caatatctgt cttcttccgt catacgtatg atggcatcca gttgtccttt
     1681 tgctgttttt aataaattaa tcacttcttt tttatcggat tctttcaatc ttcattcact
     1741 cctcattttt aataaaagca aggattaaat taaaagcccc ttttggggac ttttaattta
     1801 actattaagt gcctgatcga aatcagaaat aatatcttca atattttcaa tcccgacaga
     1861 tacacggatt aagtcttctg ttacaccaga agatatttgt tcttcttttg ttaattgcct
     1921 gtgggttgtg ctggccggat gaataacaga agttcttgcg tctgccacat gcacgactaa
     1981 agctgctaat tttacacgat taataaattt cattccagct tcttttccgc cttttacacc
     2041 aaaagttaat accccactgc agcccctagg taaatatttc ttcgctaaag cataagaacg
     2101 atggctctca agaccaggat aattcaccca ctctacctta ggatgaccct gaagaaattg
     2161 ggcaagttta agagcatttt cactgtgcct ttccattctg agatgtaagg tttctaaacc
     2221 taagttaatt aaaaatgcat taaatgggct taaggttgaa cctaaatctc ttaaaagttg
     2281 tactctggct tttacaatat atgcttgttt tccaaactgc tcaacatatt ttacaccatg
     2341 ataagtcgga tcggcttcag taaatccagg atacttgcca ttggtccagt caaaattgcc
     2401 cccatcaaca ataatccctc cgacactggt tgcatgacca tcaaaatact ttgttgctga
     2461 atgaacaacg acattggctc catgttccag tggcttgcaa agataagggg tggcaaaggt
     2521 attatcgaca ataaaaggta ctcccatttc cttagcgatg gaagaaaatt tttcaaaatc
     2581 taatatatta agacttggat tgcctattgt ttctccaaat aaggcttttg tatttggtct
     2641 gaaatgcttt ttaatctctt ctgctgaact gtccggatcg acaaaactaa cctctatacc
     2701 aaacttctta aaagtgctgg caagcaaagt aaaggtacct ccatataaat tgcttgctgc
     2761 aacgatatgt tctccggact gacaaatatt taaaatggct aaagttgtag ctgcttgtcc
     2821 tgaagctgtc gataaagccc caactcctcc ttctaactgg gctacctttt cttctaaggc
     2881 tgccagggta ggattactga ttcttgtata catatgacct tcttcttcta aatcaaatag
     2941 ctttgctaca tgttctgcac tgtcatactt atatgtagta ctttgataaa taggcaagat
     3001 tctgggttca gaattttgtg gtgtatatcc tccctgaacg gcaatggttt ctattttcca
     3061 atctttcatc cctaaacctc caattcttaa aatcaggaaa gctctccctt tggagagcta
     3121 taaccttatg ttattatatt cattatacca taaattaaac ttacaatagc atgaattttt
     3181 atataatgac aagaataatg taaatctctt ataccaaagc tgccagaagt aataattctt
     3241 cgttatcttc ataaatctta tcaaattggg aaataggtaa cggatttact ccccggccga
     3301 cttcaatggt ataacctggt tttctgtatt ctttaataaa ccagtcttta taacctgcat
     3361 ataaagcagg tcctactggt tcgtccaaac tatagccact gaccgatgcg aagatttctc
     3421 caattcttaa agcttccggc ggctgaagat ttctgtaatt ccaaaatatt acttctcctt
     3481 gagtatgata tgctaaaaca agacggaaat tacggcttct ggtaaaatcc gcaacagctt
     3541 tactttctgg ctcggattcc ggataaggtc cgccatacaa ataaggacca ggaccatcaa
     3601 tgcccaaaag ggactctacc tctttatatt cttcccagga agcatcataa tttctgttta
     3661 aatcgactcc acgaatattg gctttccaat tggaaaaatc cgtattccca ttgttccact
     3721 cgacaagttg cctatatctt gtattttcag cagatacacc gtttataacc aattcaacgc
     3781 catcaggatt taccatgggt acaatataaa tggtggcttg ttcccaaatt gttctggctg
     3841 gatatccccg catcgtacca tctcttgcaa aagctttaga aaagttttca acccatttca
     3901 ttaaaacagg acttgtaatc cattcattgg catggtgaga ggcattataa aagacttcgc
     3961 ggggtccact gccaagtttt attaaatata aattcctgcc ttctgcactt tggccaattg
     4021 tagaaacctc cagaaatgga tatcgcattt gtaaaccccg gatatctttt tccataacat
     4081 cataagtgta aggaatattg gtttgaacca aatcaattcc ataaggaaca ataatttctt
     4141 ccccaacatt caaaaaaaag ggatttatat ccggatttgc tgttgtaatt gcataaaccg
     4201 ttgtatgata ttgctgggca atattaaata atgtatctcc ttttttaatg gtatataggt
     4261 catatccaag taaaataggt tctaattttg cataggttgc tggtcctacg ataccatccg
     4321 ctgttaaagc attattcctt tgaaaagctt tcactgcttc ttctgtctga ttgccaaaaa
     4381 ttccatcaat tgcgcccggt ctatagccga ttaattgaag aatactttgt agttcagcta
     4441 cgtctgatcc tctcatccct ttcctgagaa ttctcacata tctcaccctt ttttattatt
     4501 caaatattat tcttatatta ttttataaag ttccgttaaa attggtgtct tgaaaatgag
     4561 taataacaac tctctaattt tattttattt ctctttttta aaacatagaa ttaaatagca
     4621 cgctttta
//
LOCUS       DAOSUD010000118         4621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8037_length_4621_cov_25.190758, whole genome shotgun sequence.
ACCESSION   DAOSUD010000118 DAOSUD010000000
VERSION     DAOSUD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_4621_cov_25.190758"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLG49_10350"
     CDS             complement(<1..1401)
                     /locus_tag="PLG49_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOQ17745.1"
                     /translation="MSKVIFSNDYIKLIEEDDGFYIESYKKGYTLEEFNQILSDFPQI
                     RITNFVVLRNTILNAPKFQTKFAEKRERVEITVTKDHLKAFATLYIPASELTEENREK
                     IALEILEALQDKGIVYGIIQDALLKEFKPKIRFLIAQGKLPVEGKPSVIKMYEIQEPK
                     PKITEDGKVNHYELNLINKVERGGWLGERIDATPGEPGKSVFGEEIPAKPGKQLPLLY
                     DRNSVEEVYDKEKGITTLIAKKPGAVFYKDDVIYVYDYLEIEGDVSFETGNINFDGFV
                     NIKGTIEDNFSVEAKHDIEVLGSMGIGGINKIYSKDGCIYIRGGIAGKNKARIYCKQN
                     LYTKFASDCIIECEGTVHIGFYAINCYIKAKQVIFESKNSRIIGGRIDAEIKVVANEV
                     GNRTEIPTYIHVKGFNRKKFKDTFDQINDSIKEINEDLAQLKHRAAIYRGSGNLSEKH
                     QYEFKRILKDISNLREE"
     gene            1598..4081
                     /locus_tag="PLG49_10355"
     CDS             1598..4081
                     /locus_tag="PLG49_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOQ17746.1"
                     /translation="MTIKKKKSNCLLMLLVAILLLTSCLNFNQKNKNKTIDEENNRIP
                     STESKDRYSLETGRDMTVSRALAAKMIALTYNDIGDIKSMDREIKYKDVQPGDWYDIY
                     INAVTVQGFMNGKGDTFEPLEPLTYADAQTLLERLGKGKYKIKMNLSQEESKRYITYK
                     DWLNIYLKLIAKLAEEKSIEEAYGIKERDLVVVATPANSTEVKAWQMGTDKGIYSFSG
                     FSMDSCIDKQIKVLLKDNEIFAFLGIESDAPKISNVYITDCNNKEVSIFVGGVNRTYQ
                     AIKDFSMAKEKIADIKIQTGKITNLETFEKEIRGKVLRADSSTIEIEDLGNIPLTNDY
                     KLYNISANKVQWSSLSNLIVGEDIARFIMKGDKIAAALIDKKAVPQKMRVVLHKTNFS
                     GLTHSDVRISSEDSFKVCIGGEEINFKPGEVFDIKSVEAKLSDSTPRVRIITHSPDNK
                     IKILSIQRNGDNPQYRGTIDIVKLSGGNYSIINELSLEEYLYSVIPSEMPSSYGLEAA
                     KVQAVTARSYAYSQFYSNRFHAYGGNVDDSVSCQVYNNVPENDISIQAVKATQNEALF
                     YEGNVVSANFFSTSCGYTANSGEVWADSVDKSFPSYTPPYLTAKKQFEGKDFGDLRKE
                     ENIKSFLKSKDIDAFDKEFGWFRWQVSMSLEELSASINANLKSRYEANPKLIKTLDEN
                     NIFRSRPIESIGQLKKIDIIKRGEGGNIMEMVLIGSEATIKVVTEYNVRFLLQPKQYI
                     KGRDNIIIYLHNGNKMSNYSIMPSTFFVIEPIEDKDGKLMGYTFYGGGNGHGVGMSQN
                     GVKGMVDKGYNYKEILKHYYPGTEVKKIY"
     gene            4251..>4621
                     /locus_tag="PLG49_10360"
     CDS             4251..>4621
                     /locus_tag="PLG49_10360"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOQ17747.1"
                     /translation="MEKELEILTHIQENEYITQREIAEKTGLSLGAVNLLLKKMIKTG
                     LVKIERLNARTLKYILTPEGIKEKTAKTYRYIVKTYENIVRIQAAVKVIIEKQKDKGI
                     KKIYLYGNQDEVYNILRMAVS"
BASE COUNT         1580 a          738 c          850 g         1453 t
ORIGIN      
        1 ttcttctctt agattggaga tatcttttaa aattctttta aattcatatt gatgtttttc
       61 tgagaggttg ccgctgcctc tgtatatagc agcccggtgt tttaactggg ctaaatcttc
      121 attaatctct tttatagaat cattgatttg atcaaaagta tctttaaatt ttttacggtt
      181 aaagccttta acatggatat aagttggtat ttctgtacgg tttccgactt cattggcaac
      241 aacttttatt tcagcgtcaa ttcttccgcc aataattctg ctgtttttag actcaaagat
      301 gacttgtttt gcttttatat agcaattgat tgcataaaag ccgatatgaa cggttccttc
      361 acattctata atacagtcgg aggcaaactt ggtatatagg ttttgcttgc aatagatacg
      421 ggctttattt ttaccggcaa tgccgccgcg gatatagatg cagccgtctt tgctgtaaat
      481 tttattgata ccgccaatac ccatagaacc caggacttca atatcatgtt ttgcttcaac
      541 cgaaaaatta tcttcaatgg ttcccttgat attaacaaaa ccatcaaaat taatatttcc
      601 tgtttcaaag gatacgtcac cttcaatttc taagtaatcg tacacataga tgacatcgtc
      661 tttataaaat acggcaccgg gtttttttgc aatgagtgtt gttatgcctt tttctttatc
      721 atagacttct tctactgaat ttctgtcata gagcagagga agctgtttcc ccggttttgc
      781 aggaatttct tcaccaaaaa cagattttcc cggttctcct ggtgtagcat caattctttc
      841 accaagccaa ccgcctctct caactttatt aattaagttg agctcataat ggtttacctt
      901 gccgtcttca gtaattttag gttttggttc ctgaatttca tacattttaa taacggaagg
      961 tttcccttca acaggaagtt ttccctgagc aataagaaat cttattttag gcttaaattc
     1021 ttttaacagg gcgtcctgga taataccata aacaatgcct ttgtcctgta aggcttctaa
     1081 gatttcgagg gctatttttt ctctgttttc ttccgttaat tcagatgctg gaatatataa
     1141 agtagcaaat gcttttaaat ggtctttggt aacggtaatt tcgacccgct ctcttttttc
     1201 agcaaatttt gtctgaaact ttggtgcatt gaggatggta tttcttaaga caacaaaatt
     1261 ggtaatccgg atttgcggga agtctgataa tatttgatta aactcttcta atgtgtaacc
     1321 ttttttataa gactctatat aaaatccatc atcttcttcg attaatttaa tataatcatt
     1381 tgaaaaaatg acctttgaca tattgctctc ccccccttac taaaattctc cttatctata
     1441 ttatacattg tttttacaaa taaataaatt gctaatctgt agaattataa gtttttttgt
     1501 ccttttttgc gacagggcga ggtatttaca aaaaatggca gttgtattac tataaaaata
     1561 gtagttctgc tttcattata taagggggtc aatacagatg acaatcaaaa aaaagaagtc
     1621 taattgcctg ttgatgcttt tggttgctat tttactgctt acttcttgtt taaatttcaa
     1681 tcaaaaaaat aaaaataaaa caattgatga agaaaacaat cgtattccca gcacagaaag
     1741 caaagatcgt tattctttgg agacaggcag agatatgacg gtttcaagag ctttggcagc
     1801 taaaatgatt gctttgacct ataacgatat tggagatata aaaagtatgg acagggaaat
     1861 caaatataaa gatgtccaac ccggggactg gtatgatatt tatattaatg ctgtcactgt
     1921 tcaaggcttt atgaatggaa aaggtgatac ctttgaaccc ctagaacctt taacttatgc
     1981 cgatgctcaa acgcttttag aaaggctagg aaaaggcaaa tacaaaatca aaatgaactt
     2041 atcccaggaa gaaagcaaaa ggtacattac ttataaagac tggttgaata tttatctgaa
     2101 attaattgca aaactggctg aagaaaaaag tatagaagaa gcttatggta ttaaagaaag
     2161 ggatttggtt gttgtggcta ctcctgccaa tagtacagaa gtaaaagcat ggcagatggg
     2221 aactgacaag ggcatttata gtttttcagg cttttctatg gattcttgta ttgacaagca
     2281 aataaaagtt ttattaaaag acaatgaaat ctttgctttt ttaggtatag aaagcgatgc
     2341 cccaaaaatc agtaatgtct acataacaga ttgtaacaat aaagaagttt ctatatttgt
     2401 agggggcgtc aacaggactt atcaagcaat taaagatttt tccatggcaa aagaaaaaat
     2461 tgcagatata aaaatccaaa caggaaaaat taccaatctt gaaacctttg aaaaagaaat
     2521 aagaggaaag gttttaagag cagattccag cacgatagaa atagaagact taggcaatat
     2581 tcctctgaca aacgattata aactatataa tatttctgca aataaggttc aatggagcag
     2641 tttaagcaat ttaattgttg gagaagatat cgcaagattt attatgaaag gcgataaaat
     2701 cgctgctgcc ctaattgata aaaaagcagt tcctcaaaag atgcgggtcg tcttacataa
     2761 gacgaacttt tctggcttaa ctcattctga tgtcaggatt agttccgagg atagctttaa
     2821 agtctgtata gggggagaag aaataaactt taaaccaggt gaggtctttg atattaaatc
     2881 tgttgaagct aaattatccg attctacgcc cagagttaga attattaccc attctccaga
     2941 taataaaata aaaattttat ccattcaaag aaatggagac aatccgcaat acagaggaac
     3001 aatagatata gtaaaactgt cgggaggcaa ctatagcatc atcaatgagc tttctctgga
     3061 agaatattta tatagcgtta ttccaagtga aatgccttct tcttatggtc ttgaagcggc
     3121 taaagttcag gcagttacag caagaagtta tgcttatagc cagttttatt ccaaccgctt
     3181 tcatgcttat gggggcaatg tagatgattc agtctcctgt caagtttata ataatgttcc
     3241 cgaaaacgat atttccatac aagccgtaaa agccactcaa aatgaggcct tattttatga
     3301 aggtaacgtt gtcagtgcca actttttttc cacttcctgt ggttatacag ccaattcagg
     3361 agaagtctgg gcagattctg tggataaatc ttttccgagt tatacaccac cttatctgac
     3421 tgccaaaaaa caatttgaag gaaaagattt tggagattta aggaaagaag aaaacattaa
     3481 aagcttttta aaatcaaaag acattgatgc ttttgataag gaatttggct ggttcagatg
     3541 gcaagtcagt atgagcctgg aagaattatc tgcttctatc aatgcaaatt taaaaagcag
     3601 gtacgaagcc aatccaaaac tgataaaaac cttagatgaa aataatattt ttagaagccg
     3661 tcccatagaa agtattggcc agcttaaaaa aatcgatatt ataaaaaggg gcgagggtgg
     3721 caacattatg gaaatggttt tgataggttc tgaggcaact ataaaggtcg tcacagaata
     3781 taatgttcgt tttcttcttc agcccaaaca gtatataaaa ggcagggata atattatcat
     3841 ctatttgcat aacggaaaca aaatgtccaa ttacagcatt atgcccagta ccttttttgt
     3901 gattgaacct atagaagata aagatggcaa actcatgggc tatacttttt atggcggagg
     3961 caatggtcat ggggttggta tgagccaaaa tggtgtaaaa ggcatggtgg ataaggggta
     4021 taactataaa gaaattctta aacattatta tcccggaaca gaagtaaaaa aaatatacta
     4081 aaaataaaaa cattaagcct tccagatgga aaaataagta aatctatcag gaaggccttt
     4141 ttaatatgaa aaatttgtcc agtggatgag ttaaagggaa tattcattga caaaggcatt
     4201 caggtaaagt acaataagaa tgttcaaacc atgaacaagg agcggtatac atggaaaaag
     4261 aattagaaat cctgacccat attcaggaaa atgagtatat tactcagcgg gaaatagcag
     4321 aaaaaacagg tctgtcttta ggtgctgtta atttattgct taaaaaaatg ataaaaacgg
     4381 gtctggttaa aatcgaaagg ttaaatgcca gaacattaaa gtacatatta acacctgaag
     4441 gtataaaaga aaaaactgca aaaacttatc gctatatcgt aaagacttat gaaaacattg
     4501 tccgtattca ggctgctgtc aaagtgatta ttgaaaagca aaaagataaa ggaattaaga
     4561 agatatatct gtacggtaat caggatgagg tctataatat attacgaatg gctgtatcgg
     4621 a
//
LOCUS       DAOSUD010000119         4590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8091_length_4590_cov_24.140022, whole genome shotgun sequence.
ACCESSION   DAOSUD010000119 DAOSUD010000000
VERSION     DAOSUD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8091_length_4590_cov_24.140022"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..904)
                     /gene="yabG"
                     /locus_tag="PLG49_10365"
     CDS             complement(74..904)
                     /gene="yabG"
                     /locus_tag="PLG49_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOQ17748.1"
                     /translation="MLKVGDIVVRKSYGKDLLFRIIEINRYGIVKLAGLNYRILADAP
                     WEDLEKVYPDLSYAYDEISIQKVEKNIRSILKKREEKDANRNMTRNAILKKPGKVLHL
                     DGDSEYLKICLEYYNKLNIQVIGKNIEEKEHPGRILFLLREYKPDILVITGHDSIVKG
                     TKDYKDLSNYRNSKYFVEAVKKAREYEPDMDQLVIFAGACQSNYEALIEAGANFASSP
                     ERVMIHALDPVFICEKIAYSSVQNLLSIEDILENTITGIKGIGGFQTRGKYREGAPSY
                     "
     gene            complement(990..2711)
                     /locus_tag="PLG49_10370"
     CDS             complement(990..2711)
                     /locus_tag="PLG49_10370"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOQ17749.1"
                     /translation="MELYNQWLEDTYFDEETKNELRSIADNEKEIQERFYKELEFGTG
                     GLRGIIGAGTNRMNKYTVRKATQGLANYILAHKEYDNKKGVAIAYDSRRFSKEFAQEA
                     ALVLNGNGIKTYLFKELAPTPELSFAVRHLDCIAGIVITASHNPPEYNGYKVYWSDGA
                     QVPPPYDEEIIKEVQKVTDFSQVKVKDKSEAEKEGLFNIIDEEIHDAYVEAVKAQSIN
                     GDIIKKLADNFTIVYTPLHGTGNKPVRRVLKELGFKNVIVVPEQEKPDPDFSTVSYPN
                     PEDPKAFELAIKLAKENNADIIIGTDPDADRVGVLVRDENGEYNVLTGNMTGVLLAEY
                     LLSAKKSKGTLPDNGIIIKTIVSTKMADAVAKAYNVALMEVLTGFKFIGEKIKEFEKT
                     GEYEYLFGFEESYGSLVGTYARDKDAVVASMLACELAAVYKDKGMTLYEGLQELYKKY
                     GYYKESLKSITLEGIEGQEKIQSLLASVRNNPPSEIAGMKVLEIRDYKEQVAKNLLTS
                     ETRKIVLPKSNILYFVLENEAWFCIRPSGTEPKVKIYFGVKGSSEEDANKKLDQLVND
                     IMNNIIQ"
     gene            complement(2770..4170)
                     /locus_tag="PLG49_10375"
     CDS             complement(2770..4170)
                     /locus_tag="PLG49_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOQ17750.1"
                     /translation="MDNRKTDRNNINKDRLDKPGTSKSSKSYASRRSSAKSQSDFIKF
                     MVLTGLGGLFVLLLMFGIFFSMMSGDEKKENKNNSNVSQEEKNLEDNSSSAPSGEVEK
                     ITGVVSKEAQGNVIEIVNVNTGEGIILKIDTGTELKDAYGEAMTLKEFKIGDIIQTKY
                     DKGNRVAEYFRISAEGWEKTGVKDFKIDKEAKTIEVGSNLYSYTDKIITTNKNGLITI
                     NDISSKDSLTLKGYQDKVWYINVEKSHGFLKINAADDEEGTIEIGNHISMNLSEAKNI
                     ELEEGRHKIVIKKEDYEPLVKNIDIEAGQTYEIDLKEMKKKVGKLQVIAKGLSDYKIY
                     IDDIEYPANEIILLEYGEYNLVVRKEGYKDWSRKFKIQQEMNRIELALEKEPEAGLVK
                     LVVDTDPQGAEVYIDGEYMGISPVECQLRFGQYNLSIKKEGYSPIHFPVNLEEGRKER
                     QFLFTLQEDNSTIVED"
     gene            complement(4324..>4590)
                     /locus_tag="PLG49_10380"
     CDS             complement(4324..>4590)
                     /locus_tag="PLG49_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17751.1"
                     /translation="LKKIMKKHKWDIKIASELLSIYTKKIDISEIKNILIGLLIFPDD
                     FWKLCNRYYNSRKVRAFQNPVNKLSFIVNQKENQDKFIKFIETL"
BASE COUNT         1360 a          935 c          566 g         1729 t
ORIGIN      
        1 attataccaa aatctctcat taactgtcaa tagataatag taattatgtt atacttagaa
       61 ttttcaaaaa tcgttaatag ctcggggccc cttccctata tttgcccctg gtttgaaaac
      121 cacctatacc ctttatccca gtaatggtat tttctaaaat atcctctata gaaagaagat
      181 tttgcacaga cgaataagca attttttcgc aaataaaaac agggtctaag gcatgaatca
      241 tcactctttc aggagaactt gcaaaatttg ctcctgcctc aattaaagct tcataatttg
      301 attgacaggc tcctgcaaag ataaccaatt gatccatatc aggctcatat tctcttgcct
      361 tttttacggc ttcaacaaaa tactttgaat ttctataatt acttaagtct ttatagtctt
      421 ttgtcccctt tacaattgaa tcatgccctg ttatgaccaa tatatctggt ttatattccc
      481 gcagtaaaaa taaaatcctg cctggatgct ctttttcttc aatatttttt cctattactt
      541 gtatgtttaa tttattataa tattcaagac atatctttaa atattccgaa tccccgtcca
      601 ggtgcagaac ctttcccggt ttttttaata tggcattcct tgtcatattt ctattggcgt
      661 ctttttcttc tctttttttt aagatgctcc ggatattttt ctctaccttt tgtatactga
      721 tttcatcata agcatatgaa aggtccggat aaactttttc taaatcttcc catggagcat
      781 cagccaaaat cctataattt aagcctgcaa gtttgacaat accgtagcgg tttatttcaa
      841 taattctaaa aagcaaatcc tttccatagg actttctgac aactatatcc cccactttta
      901 acatataaat tctcccttca acagttccat aattcatatt atgttgtcag aaagcttctg
      961 tgaataaaaa aatgagcaca ttaatgtgct cattgaatga tattattcat gatatcattt
     1021 actaattggt ctaacttctt attagcatct tcttcactgc ttcctttaac accaaagtaa
     1081 atttttactt ttggttctgt tccagaaggt ctgatacaaa accaagcttc attttctaaa
     1141 acaaagtata aaatatttga tttgggcaat actatttttc ttgtttcact ggtcaaaagg
     1201 tttttggcaa cttgttcttt ataatctctg atttccagca ctttcatacc cgctatttca
     1261 ctggggggat tatttctgac agaagccagc agggattgaa tcttttcttg tccttctatt
     1321 ccttcaaggg taatggattt taagctttct ttatagtagc catatttttt atataattcc
     1381 tgcaagcctt catataaagt catgccttta tccttataaa ccgctgccag ttcacaagcc
     1441 agcatggaag caacaacagc gtctttatct cttgcataag ttcccaccaa agaaccatag
     1501 ctttcttcaa agccaaaaag atattcatat tcaccagttt tttcaaattc ttttattttt
     1561 tctccaataa acttaaagcc tgttaatact tccattaaag ccacattata tgcttttgca
     1621 accgcatcag ccattttggt cgaaacaata gtcttaatga taataccatt atcaggaaga
     1681 gttccttttg atttttttgc tgataataaa tactctgcca gtaaaacacc tgtcatattt
     1741 ccagttaata cattatattc tccattttca tctctgacta aaaccccaac tctgtcagca
     1801 tccgggtctg taccaataat aatatccgca ttgttttctt ttgctaattt aattgccaac
     1861 tcaaaggctt ttggatcttc cggatttgga taagataccg ttgaaaaatc aggatcgggc
     1921 ttttcttgct ccggaaccac aataacattt ttaaaaccaa gttcttttaa aactcttctg
     1981 acaggcttat tacctgttcc atgcaaaggc gtataaacaa tcgtaaagtt atcagcaagc
     2041 tttttaataa tatccccgtt aatactttga gcttttacgg cttccacata agcatcatga
     2101 atttcttcat caataatatt aaataagcct tctttttcag cttcagactt atcttttacc
     2161 ttaacttgac taaaatcagt tactttctga acttctttta tgatttcttc atcataaggt
     2221 ggaggaactt gcgccccgtc tgaccagtat accttatatc cgttatattc cgggggattg
     2281 tggcttgctg ttatcacaat tccggcaatg cagtctaaat gtcttactgc aaaagataat
     2341 tcaggtgttg gagcaagttc tttaaataaa taagttttaa tcccattgcc atttagtaca
     2401 agagccgctt cctgggcaaa ttctttagaa aaacgccttg aatcatacgc aatggctaca
     2461 ccttttttat tgtcgtattc tttgtgtgct aaaatgtaat tggcaagacc ttgagttgct
     2521 ttcctgacag tatatttatt catccggttc gtacctgcac caataatccc tctaagacct
     2581 ccggttccaa attcaagttc tttataaaat ctttcttgaa tttccttttc attgtctgca
     2641 atagatctta attcattttt tgtttcttca tcaaaataag tatcttctaa ccattgattg
     2701 taaagctcca tgtaattcaa ctgtattacc tcctaaaaag caatataaaa atatctgatt
     2761 attttatttt taatcctcaa ctattgtact attgtcttcc tgaagagtaa ataaaaattg
     2821 tctttctttt cttccttctt ctaaattgac tggaaaatga atcggactat aaccctcttt
     2881 cttgatacta agattatatt gtccaaaacg cagttgacat tcaactggag aaatacccat
     2941 atattcgcca tcaatataaa cttcagctcc ttggggatct gtatcaacca ccagttttac
     3001 taaaccagcc tcaggttctt tttcaagcgc cagctcaatc cggttcattt cctgttggat
     3061 tttgaacttc ctgctccagt ctttataacc ttctttccga acaactaaat tatattcacc
     3121 atattcgagt aaaataattt cattggcagg atattctata tcatcaatat aaattttata
     3181 atcagaaagt ccctttgcaa tgacctgtaa ctttcctacc ttcttcttca tttcttttaa
     3241 gtcaatttca taagtttgcc ctgcttcaat atcaatattt tttaccaaag gttcataatc
     3301 ttctttttta atgacaattt tatggcgtcc ttcttctaat tcaatatttt ttgcttcact
     3361 taagttcatt gaaatatgat tacctatttc aattgttcct tcttcatcgt cagcagcatt
     3421 aatcttaaga aatccatggg atttttcaac atttatatac caaaccttat cctggtaacc
     3481 ttttaaagta aggctgtctt tagaactgat atcatttatg gtaataaggc cattcttatt
     3541 ggttgtaatg attttatctg tataagaata aagattactt cctacttcaa tagtctttgc
     3601 ttctttatca attttaaaat ctttaacacc tgttttttcc catccttcag cactaatcct
     3661 gaaatattca gccacacggt tgcctttatc atatttcgtt tgaataatat ctccaatttt
     3721 aaattctttt aaagtcattg cttccccata agcatctttt aattctgttc ccgtatcaat
     3781 cttaagaata atgccttcgc ccgtgttgac attaactatt tctatgacat ttccctgggc
     3841 ttcttttgac acaacacctg tgattttttc aacttcacca ctgggtgcac tggaagaatt
     3901 atcttccaaa ttcttttctt cctggctaac attagaatta ttcttatttt cttttttctc
     3961 atcaccactc atcatggaga aaaagattcc aaacattaaa agcaatacga ataggcctcc
     4021 cagacctgtt aataccataa atttaataaa atcagactgg gatttggcac tactacgcct
     4081 agatgcataa gacttactgc tcttagaagt ccctggctta tctaaacgat ctttatttat
     4141 gttatttcta tctgtcttgc gattatccat aaataatccc tcactttagt tcttaaaatt
     4201 cttacccgta tattatacac taataaaaac ataaaaaaaa gaaagctttt ttagcttaca
     4261 taaacagacc atcaaaaact taacttaaaa tcattaacga tttattctta ttttatatat
     4321 ttcttataaa gtttcaataa attttataaa tttgtcctga ttttcttttt gattaacaat
     4381 aaaagataat ttattaaccg gattttgaaa ggccctgacc tttctgctgt tataatatct
     4441 gttgcagagt ttccaaaagt catcaggaaa aattaaaaga ccaatcaata tattttttat
     4501 ttcactgata tctatctttt ttgtataaat ggaaagcaat tcactggcta ttttaatatc
     4561 ccatttatgc tttttcataa ttttctttaa
//
LOCUS       DAOSUD010000120         4582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8104_length_4582_cov_27.219571, whole genome shotgun sequence.
ACCESSION   DAOSUD010000120 DAOSUD010000000
VERSION     DAOSUD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8104_length_4582_cov_27.219571"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..586)
                     /locus_tag="PLG49_10385"
     CDS             complement(8..586)
                     /locus_tag="PLG49_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ17752.1"
                     /translation="MEEKRYTISEASDLLELESHVLRYWEEELNLNIPRNEMGHRYYT
                     KKEMDIFEKIKELKGRGLQLKAIKSALDDLNEKEENYSLKLAESNAINIANPNDERVK
                     KFQMMLRNLFKEALVEYNRLFKEELKEDLKRDLREEISCQIRSIELSHELKEKERYRK
                     LDEAIREVQNMRKESAVAVTVKKPWYKQVFGR"
     gene            complement(743..2074)
                     /gene="dnaB"
                     /locus_tag="PLG49_10390"
     CDS             complement(743..2074)
                     /gene="dnaB"
                     /locus_tag="PLG49_10390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ17753.1"
                     /translation="MEESKLSRIPPHDIEAEQSVLGSMIIDHEAIVTASEILRGEDFY
                     RPDHKIIYEACIDLFNQNSPIDLVTLKSRLEEMGLLEQVGGISYLSHLAASVPTSAHI
                     KQYAKIVESKSILRRLIKACQDISAVSFEAKEDVNDILNQAEQSIFHILQNKHTEDFS
                     PMREVLLSAFEKIEEIYRNQGKVTGIPTGFIDLDYKTAGFQPSDLILIAARPSMGKTA
                     FALNIAQYAAVRQKVPVAIFSLEMSKEQLVNRMLCAEAMIDSQKLRTGSLEQEDWVKI
                     ARSMGPLSEAPIYIDDTPGISVMEMRAKCRRLKLEKGLGMIFIDYLQLMTANRRFESR
                     QQEISEISRSLKALAREMEAPVVALSQLSRACEARADHRPMLSDLRESGAIEQDADVV
                     MFLYRDEYYHPDTEKKNQAEVIIAKQRNGPTGTVDLIWLGQYTKFVNMQKS"
     gene            complement(2095..2541)
                     /gene="rplI"
                     /locus_tag="PLG49_10395"
     CDS             complement(2095..2541)
                     /gene="rplI"
                     /locus_tag="PLG49_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ17754.1"
                     /translation="MKVILLKDVKNHGKEGDIVNVSDGYAKNFLIPKGLAVEATKAAE
                     NELKLKKKAEEKRKQEELDLANELRGKIEGKAISIQVKSGENGKLFGSVTSKEIAVEV
                     KNQLGLDIDKKKIQLDEPIKTLGNHIVTIKLHPKVIAQLTVKILEQ"
     gene            complement(2544..>4582)
                     /locus_tag="PLG49_10400"
     CDS             complement(2544..>4582)
                     /locus_tag="PLG49_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ17755.1"
                     /translation="NIINERKEFLEAIMGNSSNDSFFRKVFFLPQFYIVILYVFAVII
                     LLFNYMFGFTAITLCVLISLLVYRFAKKAEKKEQIIAETNENVIYENSLWDFPVPFVM
                     VDSQGLIQWYNSKFEDMIKEKTIVNKNISSFFADIRFDIFPEANKEITKEVNFDDKTY
                     KLSLYKIKQRVTFEDNEYYGIYFIDNTENAILKEESLKQKLSVGLILIDNYEEVMQNM
                     DDVRKNLLLALIDRRINAWVEEVNGILKKVDRDQYFVLFYSKYLELFKKKKFEILDQV
                     REINAGNELPVTLSIGIGINGKDLPQAMEYAKAAMDLALGRGGDQAVIKDDDKYSFYG
                     GKTREVEKSTRVKARMKAYAFRELMEETDEVIIMGHKNPDVDCLGAAVGVYRCAKFLG
                     KKTHIVLNEPNFAIKGAYDKLLESGEYEDRLFITSDEALNIIKDTTLLVIVDVHRPSY
                     TECPELLELSKKIVVFDHHRRSEEFIENAVLIYLEPFISSTCEMITEILQYVVDKIKL
                     NPLEADMLLAGITIDTKNFVFKTGVRTFEAAAFLKRNGADSTRVRMLFQNDMEAYKAK
                     AEAVRHAEIYKGDIAISVAPLNVENIAVIAAQAADELLSISGIKASFVLSVVGDEILI
                     SARSLGDINVHIIMEKLGGGGHMTVAGAQLKESSIEDVLQKLKSTIDDYLEEGE"
BASE COUNT         1331 a          978 c          580 g         1693 t
ORIGIN      
        1 ggttttttta tctgccaaat acttgcttat accaaggttt tttaacggta acagctacgg
       61 ctgattcttt tctcatattt tggacttctc tgatggcttc atctaatttt cgatatcttt
      121 ctttttcttt taattcatga cttaattcta tagatcttat ctgacaggat atttcttccc
      181 ttaaatcccg ctttaagtct tccttcaatt cttctttaaa gagcctgtta tattccacta
      241 aagcttcttt aaataaattc ctgagcatca tttgaaattt ctttaccctt tcatcattag
      301 gattggcaat attgattgca ttactctctg ctaatttcag actataattt tcttcctttt
      361 catttaaatc atcaagagca gatttaatgg ctttaagttg cagtcctctg cctttaagtt
      421 ctttaatttt ctcaaaaata tccatctcct ttttggtata atatctatga cccatttcat
      481 ttcttgggat atttaaattt aattcttctt cccaatagcg gagcacatga ctttctaatt
      541 ctaataaatc agaagcctct gatatcgtat atcttttttc ttccatttct tcacctcttt
      601 ttaattaatt tccagttata ttcatagtat accataaatt tacattaatt caataaaaat
      661 tttgtacaag ccttaaaatt tttttaaatg aaaaaagctg cccaaggcag cttaatcttg
      721 aatttaaagt tatctattaa gtttaactct tttgcatatt tacaaacttt gtatattgtc
      781 caagccaaat taaatctaca gttcctgtcg gaccatttct ctgcttagca ataatgactt
      841 cagcctgatt tttcttttct gtatccgggt ggtaatactc atccctatat aaaaacataa
      901 ccacatctgc atcttgttca attgctccgg attctcttaa atcagataac atcggtctat
      961 ggtctgctct tgcctcacaa gctctgctaa gctgtgataa agccactacc ggagcttcca
     1021 tctctcttgc cagtgctttg agagagcggg atatttctga tatttcttgc tgcctggatt
     1081 caaaccttct attggcagtc atcagttgaa ggtagtcaat gaaaatcata cctaaacctt
     1141 tttctaactt taatcttctg cactttgccc tcatttccat aactgaaatt ccaggcgtat
     1201 catcaatata aataggtgct tccgacaaag gtcccatgga tctcgctatt ttaacccaat
     1261 cttcttgttc cagagagcct gtccttaatt tttgcgaatc aatcattgct tctgcacaaa
     1321 gcattcgatt aaccaactgt tcctttgaca tttccaaact aaaaatagca acgggtactt
     1381 tctggcgtac ggcagcatat tgggcaatat taagggcaaa tgcagttttt cccatagaag
     1441 gtcttgctgc aatcagaatt aaatctgaag gctggaatcc tgcagtttta taatccaaat
     1501 caataaaacc tgtcggtatt cctgtcacct ttccctggtt tctataaatt tcttctatct
     1561 tctcaaaagc actcaataaa acttctctca tcggagaaaa gtcttctgta tgtttgtttt
     1621 gcaaaatatg aaaaatactt tgttctgcct gatttaatat atcattgaca tcttctttgg
     1681 cttcaaaact cacagcagaa atatcctgac aagctttaat taatcttctg aggattgact
     1741 tactttcaac aattttagca tattgcttaa tatgggctga tgttgggaca ctggccgcca
     1801 aatgcgataa ataactaata cctcctactt gctccaaaag tcccatctct tccagacggc
     1861 tttttagtgt caccaaatca ataggtgaat tctgattaaa taaatctatg caagcttcat
     1921 aaataatttt atggtcgggt ctgtaaaaat cctctcccct taatatttcg gatgctgtta
     1981 caatcgcctc atgatcaata atcatagacc ctaataccga ttgctcagcc tctatatcat
     2041 gtgggggaat tcttgacaat ttagattcct ccataatact ccccccttat ttttttattg
     2101 ttccaaaatt ttaaccgtaa gttgggcaat caccttcgga tgtaatttaa tcgttacgat
     2161 atgattgcct aaagttttga taggctcatc cagttgaatc tttttcttat caatatccag
     2221 gcctaattga tttttaactt ccacagcgat ttctttagaa gttactgaac caaaaagctt
     2281 accgttctcc cctgatttta cctggatgct tattgctttt ccttctattt ttcctcgtaa
     2341 ttcattagcc aaatccaatt cttcttgttt tcttttttcc tctgctttct tttttaattt
     2401 taattcattt tctgcagcct tagttgcctc aacagctaaa cctttaggga ttaagaaatt
     2461 tttagcatat ccatcactaa cattaacaat gtcgccttct ttaccatgat ttttaacatc
     2521 ttttaatagt atcactttca tactcattct ccttcctcta aatagtcgtc tatcgtgctc
     2581 tttaactttt gtaatacatc ttctatggag gattctttaa gctgtgcccc agccacagtc
     2641 atatgtcctc ctccacccag cttttccata ataatgtgta cattaatatc ccctaaagaa
     2701 cgggcactga ttaaaatttc gtctccgacc acagacagta caaaagaagc ctttatacct
     2761 gatatactca gcaattcatc tgcagcttgt gctgctataa cagcgatatt ttcaacattt
     2821 aatggggcaa ctgaaatggc aatgtcacct ttataaattt ctgcatgcct tacagcctct
     2881 gcttttgcct tataagcttc catatcattc tggaatagca ttcgtaccct tgtactgtct
     2941 gccccgtttc gctttaaaaa ggcagccgct tcaaaggttc ttacgcctgt cttaaaaacg
     3001 aaatttttcg tatcaattgt aatgcctgct aaaagcatat cggcttcaag aggatttaac
     3061 ttgattttat caaccacata ttgtaatatc tcggttatca tttcacaagt tgaagaaata
     3121 aaaggttcta aataaattaa cacagcattt tcaataaact cttcacttct tctgtgatga
     3181 tcaaaaacaa caattttctt acttaattct aaaagctcag gacattctgt ataactaggt
     3241 ctgtgaacat ccacaataac taaaagtgtt gtatctttta taatatttaa tgcctcatca
     3301 ctggtaataa acaatctgtc ttcgtattct ccagactcta atagtttatc ataagcgcct
     3361 ttaattgcaa aattgggctc atttaaaaca atgtgtgttt ttttgcctaa aaacttagca
     3421 cacctgtaaa cacctacagc agcaccaaga caatccacat ccgggttctt atgacccata
     3481 ataatgactt catccgtctc ttccataagt tctctaaaag catacgcttt cattctggct
     3541 tttacgcggg tacttttttc aacctccctg gtttttcctc cataaaaaga atatttatca
     3601 tcatctttaa taactgcctg atcccctcct ctgcctaatg ctaaatccat tgctgctttg
     3661 gcatattcca tggcctgagg aaggtctttc ccattaattc ctatacctat acttaaagta
     3721 acgggcagtt catttcctgc attgatttct cttacctgat ccaatatttc aaactttttt
     3781 ttcttaaata actccaggta tttactataa aacaaaacaa aatactggtc tctgtcaacc
     3841 tttttaagaa ttccattgac ttcttctacc caggcattta tcctcctgtc aattaaagcc
     3901 agtaataaat ttttccttac gtcatccata ttttgcatga cttcttcata gttgtctatc
     3961 agtatcagtc ctactgataa cttttgtttt aaactttctt cttttaatat ggcgttttcc
     4021 gtattgtcta taaaataaat accatagtat tcattatcct caaatgttac cctttgcttt
     4081 attttatata ggcttaactt atatgtttta tcgtcaaaat taacttcttt agtaatttcc
     4141 ttatttgctt caggaaatat atcaaagcga atatcagcaa aaaaactact gatattttta
     4201 tttacaatag ttttttcctt tatcatatcc tcaaattttg aattatacca ttgaatcaat
     4261 ccctggctgt ccaccattac aaaaggaacg ggaaaatccc ataaagaatt ttcataaatg
     4321 acattttcat tagtttcagc tattatttgt tctttttttt ctgctttttt agcaaaacgg
     4381 taaactaata aactgattaa aacacaaaga gttattgctg taaagccaaa catataatta
     4441 aataataaaa tgattactgc aaacacatat aatataacaa tataaaattg aggcagaaaa
     4501 aagactttcc tgaaaaacga atcattactg ctattaccca taattgcctc caaaaattcc
     4561 tttctttcat taataatatt at
//
LOCUS       DAOSUD010000121         4545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8180_length_4545_cov_22.920435, whole genome shotgun sequence.
ACCESSION   DAOSUD010000121 DAOSUD010000000
VERSION     DAOSUD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_4545_cov_22.920435"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..371
                     /locus_tag="PLG49_10405"
     CDS             162..371
                     /locus_tag="PLG49_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ17756.1"
                     /translation="MENNQGNKLGKKIKKLRTDLGLSQDELARKADIPYTTLTKIETG
                     VIKKPSVYVVAKIAKALNISIEELI"
     gene            387..1745
                     /locus_tag="PLG49_10410"
     CDS             387..1745
                     /locus_tag="PLG49_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOQ17757.1"
                     /translation="MNNVSLKKEDIEELKSDTKKLYSFVKTENNGRLFFVLNKLGKLP
                     NDFDDAPLLYLLDHKDAKIRYLAIKNLAKLGKFSLFPVFKEVIANDPSADVRREATSA
                     IGRLRNSKAILFLIELLKDSDPEVVMQAIRGLLVFKSNQLVAKELKKLKDHPNEIIQD
                     VIKKEFFPTSPKKRIFRHTEFPDFLRNKVVLGDVQEIIKYVPDESVHLTFTSPPYYNA
                     RDYSIYQSYEQYLKFLERVFKEVHRITKEGRFFILNTSPIIIPRVSRQHASRRYPIPY
                     DIHPLLVKMGWEFIDDIVWVKPEASVKNRNAGFLQHRKPLAYKPNARTEMVMVYRKKT
                     DKLIDWNIKQYDRKTVEESKVTGDYETSNVWEIDPVFDKTHSAVFPLELCKRVIKFYS
                     FKGDLVFDPFAGSGTLGKAAQILGRYFFLTEIDPKYFERIKENLGFIDVNYSIFNNSK
                     KL"
     gene            1750..2664
                     /locus_tag="PLG49_10415"
     CDS             1750..2664
                     /locus_tag="PLG49_10415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="HOQ17758.1"
                     /translation="MSITLSSEVTKNIIKKLLRGEDYRIEVVTLINAEFLQYAIEFFK
                     KIVDAKLKNENITADWYKKEFLNEKLSSDEIAINSGLNKKTIANMYNSARKEIVIEAA
                     FEHYETLYNSIQKLIEDDGDLNITLTIKLKGVSVDLNISESLIVINTLAVKRAALRGG
                     LWSTAGKRVEKPLMETLCLIFGVDRSNYRIKSKNTDNDLSFEREIDFYLVKGDEEYKC
                     EVKLMGKGNPESADSVIARDSKVFVADKLSQTNKNQLDHLGVEWVELRSSNGYLRFAT
                     VLDNLEIPHRNITGFTEEELENIFKQIF"
     gene            2670..3920
                     /locus_tag="PLG49_10420"
     CDS             2670..3920
                     /locus_tag="PLG49_10420"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17759.1"
                     /translation="MSIQKQFEQFYENIKLTSSQKEDAKKKYNGVCKKLHDHYYPDSE
                     YNGSTKLLIGSYGKRTNIRPPRDVDVLFIMPDDKFDQYDDNESNGQSQLLQDIKNILS
                     EKYSATEKIKGWGKVVLIQFADGTHNIELLPAWEKDNGKFIIPNTENGGFWETWDPRA
                     EINKINDSDKKTNGKTRALIRMIKKWSENCSVKLKSFQIEEKVVDFFSSNDSDREYSI
                     LVRDFFGFFCDLANEKIKSHLKTAHNRACKACHLENEDKIEEAIDEWKKIFGSGFPSL
                     KGSTKSYQEEKPALADYSHCEPLRWPFEKIYRVGIDAYIYTENKAKRLGGINSDGRVL
                     PPGLYLKFIARTNAKGIFKYYWQVVNTGEAAKRNNDLRGNIFEGNQVRGEHTKYQGKH
                     WIECFIVQNNICVARSGKFFVNIR"
     gene            3932..4528
                     /locus_tag="PLG49_10425"
     CDS             3932..4528
                     /locus_tag="PLG49_10425"
                     /inference="COORDINATES: protein motif:HMM:NF037287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HOQ17760.1"
                     /translation="MDKVNLQVIRESFGRVAYSHKTHEKAAEIEDRNSKIVKWANIVL
                     TALTSGSLVSTIVTNQNAFLYISAFLSSLMLGFTLFQLNFNPAEIAEKHRYVAKELWY
                     IREKYINLMADIINGKLSDELIISKRDQLIEELKFIYKFAPQTNSKAYEKARKALKIN
                     EELTFSDEEIDQFLTSELKLKKNLPPSSAKPSNPPLAD"
BASE COUNT         1747 a          577 c          848 g         1373 t
ORIGIN      
        1 aaaaactgcc aaagaacctg aaaaaatatc ggctcgaact ttattttggc caaaagcggg
       61 ctttttctca tctttgcctt gtaaaaattt acttgctttt attatatttg atagtataat
      121 aatgaaagaa gaaaatatca acaatattat gatatcaaaa tatggagaat aatcaaggga
      181 ataaattagg caagaaaatc aaaaagctaa gaactgatct tggtttgtct caagacgagc
      241 tcgcccgaaa agccgatata ccttatacca ctttaaccaa gattgaaaca ggggttatta
      301 aaaagccttc tgtgtatgtg gtggcgaaga ttgcaaaggc gttgaatatt tcaattgagg
      361 aattaatata aataaattta gtaaatatga ataatgtaag cttaaaaaaa gaagatattg
      421 aagaattaaa atctgacacg aaaaagctat attcattcgt gaaaacagaa aataatggta
      481 ggttgttttt tgtattaaat aaattaggta aattaccaaa tgattttgac gatgccccac
      541 ttttgtattt gttagatcat aaggatgcaa agattagata tttagctatt aaaaatttgg
      601 ccaaactggg aaaattttcg ttatttcctg tatttaaaga agttattgct aatgatccaa
      661 gtgcggatgt aagaagagaa gcgacttctg ctataggtcg attacgtaac tcaaaagcca
      721 ttcttttcct tattgaatta ttaaaagact ctgatccaga agtagtcatg caagctatta
      781 ggggccttct tgtttttaaa agtaatcagt tagttgcaaa agaattgaaa aaactaaaag
      841 atcatcctaa tgaaataatt caagatgtta ttaaaaaaga attttttcca acatcaccta
      901 aaaagagaat ttttaggcac acagaatttc ctgactttct tagaaacaaa gtggtccttg
      961 gcgatgtgca agaaataatt aaatatgtac cggatgaatc agtccatctt acttttacat
     1021 caccacctta ctataatgct agagattatt ccatttatca aagctatgaa caatacttga
     1081 aatttttgga aagagttttt aaagaagttc atagaattac taaagaaggc cgtttcttta
     1141 ttttaaatac ttcgcctatt atcatacctc gtgttagcag acaacatgcc agtaggcgat
     1201 acccaattcc ttatgatatt cacccactgc ttgtaaaaat gggctgggaa tttatagacg
     1261 atattgtttg ggtaaaacct gaagcaagtg taaaaaatcg caatgccgga tttttacaac
     1321 atagaaaacc acttgcttac aaacctaatg caagaacaga gatggtcatg gtatacagaa
     1381 aaaaaacaga taagctgata gattggaata taaagcaata tgataggaaa acagtagaag
     1441 aaagtaaagt aactggagat tacgaaactt ccaatgtctg ggaaattgat cctgtattcg
     1501 acaaaacaca tagtgcggtt tttccattag aactttgtaa aagagtgata aaattttatt
     1561 ctttcaaggg agatttagtt tttgatcctt ttgctggtag tggtacttta gggaaggctg
     1621 ctcaaattct aggtagatat tttttcttaa cagagataga tccaaaatat tttgaaagaa
     1681 ttaaagaaaa ccttggtttt atagatgtaa attatagtat ttttaataat tctaaaaaat
     1741 tgtaacaata tgagtattac attatcatca gaagttacaa aaaatattat caaaaaatta
     1801 ttaaggggtg aagactatcg tattgaggta gttactctta ttaatgctga atttttacaa
     1861 tatgcaatag aattttttaa aaaaattgtt gacgcaaaat taaaaaacga gaatatcaca
     1921 gcagattggt acaagaaaga atttttgaat gaaaaattgt catccgacga gatagctatc
     1981 aattcgggac ttaataaaaa gacaattgct aatatgtaca attcagccag aaaagaaatt
     2041 gtcattgaag cagcttttga acattacgaa accttatata attcaattca aaaattaatt
     2101 gaggatgatg gtgatttaaa cataactcta actataaagt tgaaaggagt aagtgttgat
     2161 ttaaatataa gtgagagcct tattgtcatt aacacacttg cggtaaaaag agcagcttta
     2221 agaggtggtt tatggagtac agcgggcaaa agagtagaaa agccattaat ggaaactctt
     2281 tgtttaatat ttggagtaga tagaagcaat tacagaatca aaagcaaaaa tactgataat
     2341 gatttaagtt ttgaaaggga aattgatttt tatttggtaa aaggagatga agagtataaa
     2401 tgtgaagtta aacttatggg caagggtaat ccagaaagtg cggattcagt gatagctaga
     2461 gatagtaagg tttttgtggc agataaactg tcgcaaacta acaaaaatca acttgatcat
     2521 ttaggcgttg aatgggttga attgaggtct tcaaatggat atttacgctt tgcaacagtg
     2581 ttagataatt tagaaatacc acacagaaat ataaccggat ttactgaaga agaattagag
     2641 aatatattta agcaaatttt ttagttaaaa tgagtattca aaaacaattt gaacaatttt
     2701 atgaaaatat aaaacttaca tcttcacaaa aagaagatgc aaagaaaaaa tataatggag
     2761 tgtgtaaaaa actacacgat cattattatc ctgatagcga gtataatgga agcacaaaat
     2821 tattaattgg atcttatggt aaacgcacga atatcaggcc ccccagagat gtagatgttt
     2881 tatttattat gcctgatgat aaatttgatc aatatgatga taatgaatca aacggtcaat
     2941 cacagttatt gcaagacata aagaatatac ttagcgaaaa atattctgct acagaaaaaa
     3001 ttaagggttg gggtaaggtt gtattaatcc aatttgcaga cggcacccac aatatagaat
     3061 tgcttccagc ttgggaaaaa gacaatggta agtttattat ccccaatact gaaaatggtg
     3121 gattttggga aacatgggac ccaagggcgg agataaataa aataaatgat tcagataaaa
     3181 aaacaaatgg taagacaagg gctctaatta gaatgattaa aaaatggtcg gagaactgct
     3241 cagtaaaatt aaaatccttt caaatagaag aaaaggtagt tgactttttt tcttctaatg
     3301 attcagacag agaatactca attttagtga gagatttctt tggcttcttt tgtgatttag
     3361 caaacgaaaa aataaaaagt catctaaaga ctgcgcacaa tagagcttgt aaggcttgtc
     3421 atttggagaa tgaagataaa attgaagaag ctattgatga atggaaaaag atatttggta
     3481 gcggtttccc ttccttgaag ggttcaacaa agagttatca ggaagaaaaa ccggctttag
     3541 ctgattattc gcattgcgag ccgttaagat ggccttttga aaagatatat agagtaggta
     3601 ttgatgcgta tatttataca gaaaataaag cgaaaagatt aggaggtatt aattcagacg
     3661 gaagagtttt accaccaggt ctttatctta aatttattgc aagaacaaac gcaaagggta
     3721 tttttaaata ttattggcag gttgttaata ctggagaagc agcaaaacgg aataatgatt
     3781 taaggggaaa tatttttgaa ggcaaccaag ttcgagggga acatactaaa tatcaaggaa
     3841 aacattggat tgaatgtttt attgttcaaa ataatatttg tgttgcgaga agtggcaaat
     3901 tttttgtaaa cataagataa cttaaatttt tatggataaa gttaatttac aagtaattag
     3961 agagtctttc ggaagggttg catattctca taaaacacat gagaaagcag ctgagatcga
     4021 ggatcgaaat agtaagatag tgaagtgggc taacattgtt ctaaccgcgt taacttcagg
     4081 aagtttggtt agtacaattg ttactaatca gaatgctttt ctttatataa gtgctttttt
     4141 atcaagctta atgttaggtt ttacactttt tcagttaaat tttaaccctg cagaaatagc
     4201 agagaaacat cggtatgtag caaaagagct ttggtacata agagaaaaat acattaatct
     4261 aatggctgat ataataaatg gaaaattaag tgatgaattg ataatttcaa aaagagatca
     4321 attaattgaa gaattaaaat ttatatacaa gtttgctcct caaacaaatt caaaggctta
     4381 cgaaaaagct agaaaagcgc tcaaaattaa tgaagaatta acattttcag atgaggaaat
     4441 tgatcagttt ttaacatctg agttaaaatt aaaaaagaat ttgccgccaa gctcggcgaa
     4501 gccgagcaat ccgcctttgg cggattagaa aaacttgaaa tcgtc
//
LOCUS       DAOSUD010000122         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8604_length_4354_cov_25.260060, whole genome shotgun sequence.
ACCESSION   DAOSUD010000122 DAOSUD010000000
VERSION     DAOSUD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8604_length_4354_cov_25.260060"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..404
                     /locus_tag="PLG49_10430"
     CDS             132..404
                     /locus_tag="PLG49_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17761.1"
                     /translation="MNQEYQATTGYGEQSIGVGEWLVTMLLLNIPILGLILALIWGFS
                     SNVNKSKQNFAKAYLILLIIVLILGAIFSSVIFGMLAAALGGSMSY"
     gene            complement(462..3989)
                     /gene="nifJ"
                     /locus_tag="PLG49_10435"
     CDS             complement(462..3989)
                     /gene="nifJ"
                     /locus_tag="PLG49_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ17762.1"
                     /translation="MSKVMKTMDGNTAAAHVSYAFTEVAGIFPITPSSPMAEYVDEWA
                     ANGKKNIFGQAPKVIEMQSEGGAAGTFHGSLAAGALTTTYTASQGLLLMIPNMYKVAG
                     ELLPGVIHVSARAVASHALSIFGDHSDVMACRQTGFAMLASTNVQEVIDLGSVAHLSA
                     IKGRVPFLHFFDGFRTSHEIQKVELIDYEDYKKLIDMDAVKAFRKNALNPERPVLRGT
                     AQNPDIFFQAREACNPFYDALPAVVEEYMNEINKITGRNYGLFNYYGADDADRVIIAM
                     GSVVDTIEETVDYLNSKGEKVGLVKVRLYRPFSIEHFLKVVPKTVKKIAVLDRTKEPG
                     AIGEPLYLDVCTAFMGQSSQPVIVGGRYGLGSKDTTPAQIVAVFENLKQDEPKNNFTI
                     GIVDDVTHKSLPTGEELDITGEGTISCKFWGLGSDGTVGANKNSIKIIGDHTDMYVQA
                     YFSYDSKKSGGVTISHLRFGKKPIRSTYLIKKADFVACHNPSYVDKYDMVSDLKTGGT
                     FLLNCDWTVEELEEKLPAKMKKILAERKAKFYIIDGVSIAKEIGLGNRVNTVLQAAFF
                     KLANIIPIEDAVKYMKDAIVASYGKKGEKVVNMNFAAVDRGVVDIVEVKVPAHWTDLS
                     VEEEAAATKDVPEFVAKIMEPMNRQEGDNLPVSAFVGREDGTFPQGTSKYEKRGIAVE
                     VPEWQMDKCIQCNQCSFVCPHAVIRPFLLNEEEVNNAPEGFTSKKAIGKGLENYQYRI
                     QVSVLDCTGCGSCAQVCPAKEKALIMKPIETQTAQIENWEYAMTLSKKENPLNVATVK
                     GSQFEQPLLEFSGACAGCGETPYAKLLTQLFGDRMYIANATGCSSIWAGSAPSTPYTT
                     NEKGHGPAWANSLFEDNAEFGFGMYMAVKQIRNKLKDLAEEAQSLDVSDEIKVALKDW
                     VDNMYDAAGSKKATLTLVPLLEAYNGSGRAKEIFEEILEKKEFLVKKSHWILGGDGWA
                     YDIGFGGLDHVLASGEDINVLVFDTEVYSNTGGQSSKATPTGSIAKFAAAGKPIKKKD
                     LGRMMMSYGYVYVAQVAMGANQNQLIKALIEAESYPGPSLIIAYSPCINHGIKGGLTC
                     SQEETKKAVEAGYWHLYRYNPLLKEEGKNPFILDSKVPTADYREYIMNEVRYSALTRT
                     FPEVAEKLFEQSEKDAKERFEAYQKLAEQ"
BASE COUNT         1298 a          852 c          675 g         1529 t
ORIGIN      
        1 attcaaaaga tgcaatccca agaagtttac tgaaaattgc gagtttataa tttaatctca
       61 aaaattgttg acattataaa aattatgtta tttaataatc ttataagatt actataagga
      121 ggattataat tatgaatcaa gaataccagg caacaactgg ctatggcgag caatctatag
      181 gtgttggtga atggttagta acaatgcttc ttctaaacat tcctatatta ggtcttattc
      241 ttgctcttat atggggattt tcttccaatg ttaataagag caaacaaaat ttcgctaaag
      301 cataccttat tttgttgata atcgttctaa ttttaggagc aatattctct tcagttattt
      361 ttggtatgtt agcagcagct cttggtggtt caatgagcta ttaatattta ttaaaaaata
      421 tataaaaaag cttctgtgtt ttacacagaa gcttttttat tttattgttc tgctagcttt
      481 tgatatgctt caaatctttc tttagcatct ttttcggatt gttcaaatag tttttctgct
      541 acttcaggga aggttcttgt aagagcagaa tatcttactt cattcattat atattcccta
      601 tagtcagcag ttggtacttt agaatctaag ataaatggat tctttccttc ttctttaaga
      661 agaggattat atctataaag atgccaataa ccagcttcta cagctttttt ggtttcttcc
      721 tgagaacaag tcaaaccacc tttaattcca tggttgatac atggtgaata agcaataata
      781 agtgatggtc ctggataact ttctgcttca attaaagctt tgattaactg attttgattt
      841 gctcccatag caacttgtgc tacgtaaaca tatccataac tcatcatcat tcttccaagg
      901 tctttcttct tgattggttt tcctgctgca gcaaatttag caatggaacc agttggtgta
      961 gcttttgaag attgtccgcc tgtatttgag tatacttctg tatcaaatac aagaacgtta
     1021 atatcttcac cagaagctaa tacatgatca aggccaccaa atccaatgtc ataagcccaa
     1081 ccgtctccac ctaaaatcca gtgagacttc ttcactaaga attctttctt ttcaagaatt
     1141 tcttcaaaaa tttcttttgc tctgccacta ccattgtaag cttctaataa tggcactaat
     1201 gttaaggtag ctttctttga acctgcagca tcatacatat tgtctaccca atccttaagg
     1261 gcaactttga tttcatcaga aacatctaag ctttgagctt cttctgcaag atcttttaat
     1321 ttgtttctga tttgttttac agccatatac ataccaaagc caaattcagc attgtcttca
     1381 aacaaggaat ttgcccatgc tggtccatga cccttttcgt tggttgtata tggagttgaa
     1441 ggtgcacttc ctgcccaaat ggaagaacat cctgttgcat ttgcaatata cattctatct
     1501 ccaaacaatt gagttaacag cttagcatat ggtgtttcac cacaacctgc acaagcaccg
     1561 gagaattcaa gcaatggttg ttcaaattga ctgcccttaa cggttgcaac atttaatgga
     1621 ttttccttct tagataaggt cattgcatat tcccaatttt caatttgtgc tgtttgagtt
     1681 tcaattggct tcataattaa tgctttttct ttagcaggac atacttgggc acagcttccg
     1741 caacctgtac agtctaaaac agatacctga attctgtatt gatagttttc taaaccttta
     1801 ccaattgcct tcttggatgt aaatccttca ggtgcattat taacttcttc ttcattgagt
     1861 aagaaaggac ggataactgc atgtggacat acaaatgaac attggttaca ttgaatacat
     1921 ttatccattt gccattcagg tacttctaca gcaattcctc tcttttcgta ttttgaagta
     1981 ccttgtggga aggttccatc ttctctgcca acaaatgcgc taactgggag attgtctcct
     2041 tcttgtctgt tcataggttc cataatctta gcaacaaact ctggtacatc ttttgtagcc
     2101 gctgcttctt cttctacgga aagatctgtc cagtgagcag gaaccttaac ttctacaata
     2161 tctactacac ctctgtctac agcagcaaag ttcatattaa caactttttc acctttttta
     2221 ccataagaag ctacaatagc atctttcatg tattttactg cgtcttcaat tggaataata
     2281 ttcgctaatt taaagaatgc tgcctgtaat acagtattta cacggtttcc taggccaatt
     2341 tcctttgcaa tggaaacccc gtcaatgata tagaatttag cttttctttc tgcaagaatc
     2401 tttttcattt ttgcaggtaa tttttcttct aattcttcta cagtccagtc acagtttaaa
     2461 aggaatgtgc cgcctgtttt taagtcagat accatatcat atttatctac ataagaagga
     2521 ttatggcatg caacaaaatc tgccttctta attaaatatg tggagcggat tggtttttta
     2581 ccaaaacgga ggtgagaaat cgttacacca ccagacttct tggagtcata ggagaaataa
     2641 gcctgtacat acatatctgt atgatctcca ataatcttaa tggagttttt attcgcacct
     2701 acagttccgt cagagccaag tccccagaac ttgcagctaa ttgttccttc tcctgtaatg
     2761 tctaattctt caccagttgg aagagattta tgggtaacat catcaacaat accaatagta
     2821 aagttgtttt taggttcatc ctgcttaagg ttttcaaata ctgccacaat ttgagcaggt
     2881 gtagtatctt ttgaacctaa gccgtaacgg ccaccaacga taaccggttg actggattga
     2941 cccataaatg ctgtacatac atcaagatat aatggttctc caattgcacc tggttctttt
     3001 gttctgtcta atacagcaat cttcttcact gtcttaggta ctacctttaa gaaatgttca
     3061 attgagaaag gtctgtataa acgtaccttt acaagaccca ccttttctcc cttgctgttc
     3121 aagtaatcta cagtttcttc aatagtgtct actacagaac ccattgcaat aattactctg
     3181 tcagcatcat ctgctccata ataattaaat aatccataat ttcttcctgt aattttgttg
     3241 atttcattca tatattcttc tacaacagca ggaagcgcat catagaaagg attgcatgct
     3301 tctcttgcct ggaagaagat atctggattt tgtgctgttc ctctaagtac aggacgttca
     3361 ggatttaaag cattctttct gaatgctttt acagcatcca tatcaattaa ttttttgtag
     3421 tcttcatagt ctattaattc aactttttgt atttcatgag atgttctgaa tccatcgaag
     3481 aaatgaagga aaggtactct tcctttaatc gcactaaggt gtgctactga gcctaaatca
     3541 ataacttctt gtacgtttgt tgacgctaac attgcaaaac ccgtttgacg gcatgccata
     3601 acgtctgaat gatctccaaa aatggaaagc gcatggcttg caacagcacg ggctgaaaca
     3661 tgaataacgc caggtaaaag ttctcctgca accttataca tattaggaat catcagcaat
     3721 aatccctgag aagctgtata agtagttgtt aatgcacctg ctgctaaaga accatgaaag
     3781 gtaccagctg ctccgccttc tgattgcatc tcaattactt taggtgcctg accaaaaata
     3841 tttttcttac cgtttgctgc ccattcatca acatattcag ccataggtga agatggtgtg
     3901 attgggaaaa taccagctac ttcggtaaat gcataggaaa catgcgcagc tgccgtattt
     3961 ccatccattg tcttcatcac ttttgacatc attttacccc ctagaataat ataattattt
     4021 aaatacaata atatcagtat attgtataca catattataa catagaaaca caattagtaa
     4081 atagatttct ttagcttttt cccaatacat gataacatta taacaatctt ttttcaatat
     4141 ttccgtcagc ctgttattac aattttatct ttaaaaatct tcctttagct gccctatttt
     4201 aagaatttct aaggctacga caaatttcaa tcttattttt aaatcatgat taaataaaaa
     4261 cactaatata aggatatatt agtgtctgtc aaatttttta taaaattaat ttaaacttat
     4321 tgaatgatat agtcattaat aatttgataa actt
//
LOCUS       DAOSUD010000123         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8653_length_4329_cov_25.552176, whole genome shotgun sequence.
ACCESSION   DAOSUD010000123 DAOSUD010000000
VERSION     DAOSUD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8653_length_4329_cov_25.552176"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1792
                     /locus_tag="PLG49_10440"
     CDS             77..1792
                     /locus_tag="PLG49_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOQ17763.1"
                     /translation="MIELLQELLNKLSIVVFFAFFLTKSSLFQRYFLKKKINMADKIF
                     FSIAFGCIGILATYWGIPIENTLANSRSISILLAGILGGPLVGTGAGLIAGIHRMLIP
                     EGRFTAIACGISTILAGVIAGISKKYIDEKQKKWPYGLVLAIIVECLQLLLILLISKP
                     FDKALQVVKILFFSMTLINVLGITMILMIIEQFFGEQEKAGSEKAQLALNIATQTLPY
                     LRKGLTEQSALNTANIIYSMTDASAVAITDTNKILVHVGLGSDHHKKNDPIDTKITRD
                     VLSRGLFMVAQEKCEINCSNEDCPLKSVIVVPLKMQDNIIGSLKIYKSSGNSINPSDI
                     ELAKGLGHLFSTQLELSQIDYQKELLIESEIKALQAQIQPHFIFNALNTIAVFCRTNP
                     NKARELILNLSNYLRNSFKNQGEFIPLSKEIESVQAYLSIEQARFSERLNVAYDIKAD
                     MDIFIPPLILQPLVENAIKHGILSNIEGGTVKISAFSEDDEAVIIVEDTGVGMSKTQI
                     HALLNNKYSNQGVGLTNVINRLQCIYGRKPEIESEIGKGTKITIKIPKIPKERGELFE
                     NSISG"
     gene            1770..2528
                     /locus_tag="PLG49_10445"
     CDS             1770..2528
                     /locus_tag="PLG49_10445"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ17764.1"
                     /translation="MRIVLVDDEKPALEELSYIINKYSFIEISGTFTNPAEALEHILK
                     EKPDVVFLDIEMPEINGFTIAREITNAEIDTIIVFVTAFDEYALKAFEVNAVDYILKP
                     YDEKRIDQTVKRLKNISKQQKKENSQKVIQKILEMQELRPINKLPVWKNNRILLLNPE
                     DILYCTMEDGKSIIVTENERYVSDGKLIYLEEILRRDGFFRCHRSFLVNLNAISEVIP
                     WFNNTYIIKVRGLEDEIPISRRHMKEFKKKLNIS"
     gene            complement(2801..3220)
                     /locus_tag="PLG49_10450"
     CDS             complement(2801..3220)
                     /locus_tag="PLG49_10450"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ17765.1"
                     /translation="MKKTGQKKFDNYSTIIGENTILESEYLKSNETVRIDGKYKGEII
                     LESDLVIGEKGLVEGSVKANNIEISGTVVGDINSKELVYLTATAKVKGNISCNNLVID
                     DGAKISGFCNSGNLSNSSDAITVNDNQETKSKKNKAV"
     gene            complement(3232..4233)
                     /locus_tag="PLG49_10455"
     CDS             complement(3232..4233)
                     /locus_tag="PLG49_10455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ17766.1"
                     /translation="MKRNKKRKHLLSNRFTIMIVPNSSKKVIKLSLPHWMSNILVSFF
                     FILILALCSSIGYLFYQQSQIAHYTNIIEEQKAEIAALMKKNENYLAELTSLQDKTEE
                     VINQLETLNHFKKMIYEKVNKSTNNTMQESLSKIEYYEYSPYESSGGMISYQGGGETF
                     DFDEIAKNLKETVDSSKYKVDTEFKELKDLDQFIEEITPYLEAYPSILPAKGRITSEM
                     GWRRNPFSSSRSEFHAGIDIAADIGTDVVATGNGTVIYAAYKNGYGYLVIIDHGFGIH
                     TYYAHNSSLLVEEGDNVKRGDIIAKSGSTGYSTGPHVHYEIRLNNVPQDPLEYIINH"
BASE COUNT         1456 a          624 c          766 g         1483 t
ORIGIN      
        1 ctgaattaat ataaaaaata gatttgtcat attaacaata aaactatttt tattagaaat
       61 agtaggggat gaaatgttga tagaactatt acaagaatta ttgaataagt taagcattgt
      121 tgtatttttt gcatttttcc tcacaaaatc aagtttgttt caaagatatt ttttgaagaa
      181 aaaaataaat atggcggata aaattttctt ttctattgcc ttcggttgta ttggaatatt
      241 ggcaacttat tggggtatac ctattgaaaa taccttagcc aattcccgtt caatcagtat
      301 acttttagca ggtattctgg gtgggccttt ggtaggaacg ggagcaggtt taattgccgg
      361 gattcaccgg atgcttatac ctgaaggcag gttcactgcc atcgcttgtg gaatatctac
      421 aattttagct ggagttattg ctggaatttc taaaaagtat attgatgaaa aacagaaaaa
      481 atggccttac ggacttgttt tagcaattat tgtggaatgc ttacaattgt tgttaatttt
      541 attaatttca aaacctttcg ataaagccct gcaagttgtg aagattttat tcttttcaat
      601 gaccctcatt aatgtgttgg gaattactat gattcttatg ataattgagc aattttttgg
      661 tgagcaagaa aaagcaggca gtgaaaaggc ccagttggca ttaaatattg ctacccagac
      721 cttgccttat ttaagaaaag gtctgacaga acaatctgct ttaaatacag ccaatattat
      781 atattccatg accgatgctt ctgcagttgc tataacagat acgaacaaaa tattggttca
      841 tgtaggttta ggttcggatc atcacaaaaa aaatgatcct atagacacaa aaattacaag
      901 agatgttttg agcagaggct tgtttatggt tgcccaggaa aagtgtgaaa ttaattgcag
      961 caatgaagat tgtccgttaa aatcagtcat tgttgtaccc ttaaaaatgc aggataacat
     1021 tataggaagt cttaaaatat ataaaagttc aggaaacagt attaatcctt ctgatattga
     1081 attagccaaa ggtttaggtc atttattttc aactcaattg gaattaagcc aaattgacta
     1141 tcaaaaagaa ttattaatcg agtcagaaat caaggctttg caagcgcaaa tacaaccgca
     1201 tttcatattt aatgcattaa atacaatagc agttttttgc cggacaaatc ccaataaagc
     1261 aagagaatta atcttaaatt tgagcaatta cttaagaaat agttttaaaa atcaaggaga
     1321 gtttattcct ttgtcaaaag aaatagaatc agttcaggct tatttatcca tagagcaggc
     1381 ccgtttttct gaacgactga atgttgctta tgatataaag gcggacatgg atatctttat
     1441 accaccgctt attttacagc ctctggtgga aaatgcaatt aaacatggta ttctttctaa
     1501 tattgaagga ggtacggtaa aaatttcagc tttttctgaa gatgatgagg cagtcattat
     1561 cgttgaagac acaggcgttg gaatgagtaa aactcaaatc catgcattgc tgaataataa
     1621 gtatagtaat cagggggttg gtctgacaaa tgttattaac cgccttcaat gtatttatgg
     1681 cagaaagcca gaaatcgaaa gcgagattgg aaaaggaaca aaaataacca tcaagatacc
     1741 taaaatacct aaagagcggg gtgaactttt tgagaatagt attagtggat gatgaaaaac
     1801 cggcattaga ggagctaagc tatattatta ataaatatag ttttatcgaa atatcaggta
     1861 cttttacaaa tcctgccgaa gccttggagc atatccttaa ggaaaaaccc gatgttgtat
     1921 ttttagatat cgaaatgccg gagatcaatg gttttactat tgcaagagaa ataaccaatg
     1981 cagaaataga taccatcatt gtttttgtaa cagcttttga tgaatatgct cttaaagctt
     2041 ttgaggtaaa tgccgtagat tacattttaa agccttatga tgaaaaaaga atcgatcaaa
     2101 ccgtcaagcg tttaaaaaat ataagcaagc agcaaaaaaa agaaaacagt cagaaggtca
     2161 ttcaaaaaat actcgagatg caagagttaa gaccaatcaa caagttgccc gtttggaaaa
     2221 ataaccgaat attgttatta aatcctgaag atatattata ttgcaccatg gaagatggta
     2281 aaagcattat tgttacagaa aacgaaaggt atgtttcgga cgggaaactc atttatttag
     2341 aggaaatttt aaggcgggat ggctttttta gatgccacag aagtttctta gttaatttaa
     2401 atgcaattag tgaagtcata ccttggttta ataatacgta tattattaaa gtaagaggtt
     2461 tagaggatga aataccaatt agtagaaggc atatgaaaga atttaagaaa aaattaaaca
     2521 tttcttgacc gtttaaatga atattttacc tttcaaatta ttttttttgt cttttataga
     2581 ttatttttat atttttatat tgaagcatgt tattatatag atggtggttg ctgtatatgg
     2641 tgaatttggg gtatgtaaaa agatttttta ctgatttgga tggggacaaa aacttagaga
     2701 gagaaaattg atcgtgattt tgttatgttg ggactcatat tcttttataa gacgatttaa
     2761 ccttctacta atagtagaag gttttttctt tgaaaaaaaa ttatacagct ttattctttt
     2821 ttgattttgt ttcctgatta tcatttacag tgattgcatc agaactattt gataagtttc
     2881 ctgaattaca aaaacctgat atttttgcac cgtcgtcgat gactaagtta ttacagctaa
     2941 tattaccttt aacttttgca gtggctgtca gatatactaa ctcttttgaa ttgatatctc
     3001 ctacaactgt tccgcttatt tcaatgttat ttgctttcac agaaccttcg acaagtcctt
     3061 tttctcctat aactaaatca ctttctaata taatttctcc tttatattta ccatcaatac
     3121 ggactgtttc attacttttt agatattcag attctaaaat tgtattttct ccaatgatgg
     3181 ttgaataatt gtcaaatttt ttttgtccag tctttttcat agaaattcct cctaatgatt
     3241 tatgatatat tctaaaggat cttgagggac attatttaaa cgaatttcat aatgaacatg
     3301 aggaccggtg ctatatccgg ttgaaccaga tttagcaatg atatcaccgc gtttaacgtt
     3361 atctccttct tccactaaga gggaagaatt gtgggcatag tatgtatgta ttccaaagcc
     3421 atgatcaata atgactaaat aaccataacc atttttataa gcagcatata tgacggtacc
     3481 gtttcctgta gcaacaacgt ctgtgccaat atcggcagca atatcaattc cggcatgaaa
     3541 ttcacttctg gaagaactaa agggatttct cctccaaccc atttctgaag ttatcctccc
     3601 tttagctgga agaatagaag gataagcttc caaataaggg gtaatttctt caataaattg
     3661 atctaaatct ttaagttctt taaattcagt gtctacttta tatttacttg aatcaacagt
     3721 ttccttaagg ttttttgcaa tttcatcgaa gtcaaaagtt tcgccgcctc cctgatagga
     3781 aatcattcca ccagagcttt cataagggga gtattcatag tattctattt tgcttaagct
     3841 ttcctgcatc gtattattgg tggatttatt gactttttca taaatcattt ttttaaaatg
     3901 attaagggtt tccaattggt tgataacttc ttcggtctta tcttgtaaag aagttaattc
     3961 agcaaggtaa ttttcgtttt ttttcattag ggcagctatt tcggcttttt gctcttcgat
     4021 gatgttggta tagtgggcaa tttgactttg ttgataaaaa agatagccta tggaagaaca
     4081 aagagcaaga ataagaatga aaaagaatga tacgagtata tttgacatcc agtggggtaa
     4141 agacagcttg atgacttttt ttgagctatt cgggacaatc ataattgtaa aacggtttga
     4201 aagcaggtgt ttacgttttt tatttctttt cattggttat caccttaaaa attacttttt
     4261 aattattaag aattgatgat aaaaatataa aatattataa catgaattga taaaaaatgt
     4321 aacaatttt
//
LOCUS       DAOSUD010000124         4216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_8903_length_4216_cov_26.485701, whole genome shotgun sequence.
ACCESSION   DAOSUD010000124 DAOSUD010000000
VERSION     DAOSUD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_4216_cov_26.485701"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            670..2496
                     /gene="glmS"
                     /locus_tag="PLG49_10460"
     CDS             670..2496
                     /gene="glmS"
                     /locus_tag="PLG49_10460"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOQ17767.1"
                     /translation="MCGIVGYIGNKKAKPILIDGLKKLEYRGYDSAGIAILNSGEINV
                     VKSKGRIAVLEDKLKDISLEGTIGIGHTRWATHGEPSDINSHPHISKNGKIAVVHNGI
                     IENYIQIKEFLLAKGYQFKSETDTEVIAHLIEYNYEGDLIKAVIKTLNELEGAYALGV
                     LSTDNPDMFVAAKKDSPLIVGLGQNENYIASDIPAILKYTRDILILEEKEVACISSDK
                     VEIYNLLGEKQQREVFHVDWDVSAAEKGGFEHFMIKEIFEEPAAIKATISPRIKDNEI
                     VLDNLIITDEIINNCEKIHIIACGTAYHAGVVGKYIIEKLARIPVEVEVASEFRYRDP
                     IINEKHFTIVISQSGETLDTLMAMREAKKKGSLTFAIVNVVGSSIAREADDVLYTWAG
                     PEIAVASTKAYNTQLAALYLLALTFAKRKGLLDQHSYLNYLSQLQELPNMITSVLNEK
                     KEIQKFASEHFNAKSIFYIGRGLDYALSMEGSLKLKEISYIHSEAYAGGELKHGTIAL
                     IEEGTLVVASLTQEQLFEKMIGNIREVKARGAVVLAIAQEKNADEVAKIADAVITIPN
                     VDSVLAPIVAVTTMQLFAYYVAVMKGNEVDKPRNLAKSVTVE"
     gene            3554..4111
                     /locus_tag="PLG49_10465"
     CDS             3554..4111
                     /locus_tag="PLG49_10465"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOQ17768.1"
                     /translation="MSRVNLEPKPFMYPMPVLIIGTYDENGVPNAMNAAWGIITDYQE
                     ITISLAKEHKTAKNLLATKAFTVSIATEDMVIPCDYVGIESGNKVPNKLEIAGFHARK
                     SEFVNAPLIEELPMTLECKVKSYEDEILVGEIVNISVDERILTDGKIDPKKLKPITYD
                     PVNHAYISLGEKVGNAFKDGLKLKK"
BASE COUNT         1497 a          596 c          858 g         1265 t
ORIGIN      
        1 ccgtataatt ccctatttat gataattata ggtattagct atctattaaa ttcaatatat
       61 tctaagtata ttatggttta ttaaaaattt gataaaaata aatatatgta acatttattt
      121 ctttcgataa ttttcaactt tagaaggaaa cagaagaaag gtttctttac ttttctatgt
      181 ttcaatagtt gaagaaagat taaaataatt ctgcgctttt gtattttata gcaaaaaaaa
      241 atgttataga atataataga tagggaatgc agattggaac tgtcgggaag gaggtgtatg
      301 atttaagaag acagtaaata aagagcgcca gaactaagga ttatcttatc cttagttgac
      361 gaggagaggg cagtgatcga agttttcggc ggaaaccctc cggtgtgcca ccaccgaata
      421 aatactacaa atcctgatgg cgacatcagg gacagaatgt atttaagtgg tcccttctgc
      481 atttcttaat attcccattt aatgatcaaa ttttatctgg tttaatatga aatgtcagat
      541 ttttgaagtt tctggcctta cttttgtgta ctcatttttt gagtgctata ttagctaagg
      601 tctggaaagt gtgtctgcaa atatttcagc cagattaata aaattaagaa atcatgaagg
      661 agcgttaaaa tgtgcggaat agttggttat attggaaata aaaaagcaaa acccatactt
      721 atagatggtc ttaaaaagct tgaatacaga ggttatgact ctgcaggtat tgcgatttta
      781 aacagtggag aaatcaatgt tgttaaatca aaaggacgaa ttgctgttct tgaagataaa
      841 ttaaaggaca taagtcttga aggaacgatt ggtataggac atacccgttg ggcaacccat
      901 ggagaaccca gtgatatcaa ttcacatcct catataagta aaaacggaaa aattgcggtc
      961 gttcataacg ggattataga aaactatatt caaattaagg agtttttatt ggctaagggc
     1021 tatcagttta aatcggaaac agatacagaa gtgattgccc acctcattga atataattat
     1081 gaaggtgatt taataaaagc tgttataaaa actttaaatg aattagaagg agcttatgcc
     1141 ctgggggtac tgtctactga taatcctgac atgtttgtcg cagcaaaaaa agacagtcca
     1201 ttgattgtag gtcttggtca gaatgaaaat tatattgcat cggatattcc tgcaatcctg
     1261 aaatatacgc gggatatatt aattctcgaa gaaaaggaag ttgcatgtat tagtagtgac
     1321 aaggttgaaa tttataacct gctgggagaa aaacagcagc gtgaagtatt ccacgtggac
     1381 tgggatgttt cagcagccga aaaaggtgga tttgaacatt ttatgataaa agaaatcttt
     1441 gaagaacctg cggctataaa agcaaccatt agtcccagaa tcaaagacaa tgaaatagta
     1501 ttggataact tgataatcac cgatgagatt atcaataact gcgaaaagat tcatattatt
     1561 gcctgtggaa cggcctatca cgcaggagtt gtaggaaagt atattatcga aaagcttgca
     1621 agaattcctg ttgaggtaga agtcgcatcg gaattcagat acagggatcc tattattaat
     1681 gaaaaacatt tcaccatcgt tatcagtcaa tcaggtgaga ctcttgatac gctcatggca
     1741 atgcgtgaag ccaagaaaaa gggctcatta acctttgcta ttgtcaatgt agttggaagt
     1801 tccattgcaa gagaagcaga tgatgtgttg tatacctggg cgggtcctga aatagcagtc
     1861 gcttcaacaa aggcatacaa cacccaactt gcagccttat accttttggc tttgactttt
     1921 gcaaaaagga aaggtcttct tgatcagcat agttatctta actatttgag ccagctgcag
     1981 gaactaccca atatgataac aagtgtatta aacgagaaaa aagaaataca aaaatttgcc
     2041 agtgagcatt ttaatgcaaa aagtattttc tatataggca ggggcttaga ttatgctcta
     2101 tctatggaag gttccttaaa attaaaagaa atttcctata tccattcgga agcctatgct
     2161 ggaggagaac tcaagcatgg aaccattgcc cttattgaag aaggaacttt ggttgttgct
     2221 tccttaactc aggaacagct ttttgaaaaa atgataggaa atattcgtga agtcaaagca
     2281 agaggtgctg ttgtgctggc tattgcccag gagaaaaatg cagacgaagt tgccaaaata
     2341 gcagatgcgg ttattaccat tccaaatgta gattctgttt tggcaccaat cgtagccgta
     2401 acaaccatgc agctttttgc ttattatgtg gctgtcatga aaggtaatga agtagacaaa
     2461 ccaagaaatc tggctaaaag tgtgacggtg gaatgaaaat aaacttcact ttataaaaat
     2521 attgttgttg tataatacaa ataaactcca ctccgtaata ataaacttcg ctctataaaa
     2581 ataaagttca ttccaaaatc ccgtagtaga tgcaggagaa atcttcattt gctgcgggtt
     2641 ttgtttttgc aagcaaaagg ggctgaaagg gttaaggtat aagggataag actgaaggta
     2701 ttgattttaa aggtattaag agtattaatt attgataccg gaattgaaaa aagaattgaa
     2761 gaaatacata aaaaaatgaa accttatacc tttttccttt caccttatcc ctatttggaa
     2821 ccgactttat tatatggaaa gaattagggg agtgggcata aggcgagaag ggataagggt
     2881 ttgagggaaa atcgtttgga atggagttta ttttaaatat acaaaatata gcttttctaa
     2941 aatcccgtag atagtgaaga aaaatcttcg cttctgcggg gtttgctttt gttgagatta
     3001 aagcttaaga tttttgcaat taagcgtaga aaaattaata tggaatagcc aagttgactc
     3061 tgtaataatc aaatagatct tattataata gagttataaa aaagtagata ttcttaaaat
     3121 ttatagtgaa aaataaaatt atgtattaaa tatttacagg atatttcgag agattatttc
     3181 cctgagggaa agagagaacg attatgaaga aatggacttt gctggtgttt atcatttcat
     3241 gttgctttgc ctgaagactc gcttaaaggt aagcaaatag gaattattga tggtgacaaa
     3301 aagcataagg tctttgagct aaaaggttat tcatccgatg aatgaattat agaatattac
     3361 gatgttatga tgtcagctta taatttatat aaagcagata cagtaaccaa aatacctgac
     3421 gaattaaaat agcattcaaa aaagtaagga gacgtttctc ttgcttctgc attgaacagg
     3481 ttttcccaaa agagcaggag aattgttctt tatttttctt aaaaatagtt tttactaagg
     3541 agtgaagtta gaaatgagta gagtaaattt ggaacccaaa ccttttatgt atcccatgcc
     3601 agttttaatt ataggcacat atgatgaaaa tggtgttccc aatgccatga atgcggcctg
     3661 gggaatcata acggattatc aggaaataac gattagttta gcaaaggagc ataaaacagc
     3721 aaaaaattta ttagctacaa aagcttttac ggttagcatt gcaacagaag atatggtaat
     3781 tccctgtgat tatgttggga ttgaatcagg gaataaagtc cccaacaagc ttgaaatagc
     3841 aggctttcat gcaagaaaaa gtgaatttgt aaacgcacct ctgattgaag agttgccgat
     3901 gacccttgag tgtaaggtaa aaagttatga ggacgaaatt cttgttggtg aaatcgtcaa
     3961 tattagtgta gacgaaagaa ttttaacaga tggcaaaata gatcctaaaa agctaaagcc
     4021 aattacgtat gatccggtaa atcatgccta tataagtctt ggagagaaag taggcaatgc
     4081 atttaaggat ggtttgaagt tgaaaaaata gcaaaaataa aatacgggga cgttcgttaa
     4141 attgctttcc catcctttct cttgacatca tgaagttaat atattagact tagtaattaa
     4201 tcagataaaa attaaa
//
LOCUS       DAOSUD010000125         4156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9022_length_4156_cov_24.365764, whole genome shotgun sequence.
ACCESSION   DAOSUD010000125 DAOSUD010000000
VERSION     DAOSUD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9022_length_4156_cov_24.365764"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..988)
                     /locus_tag="PLG49_10470"
     CDS             complement(5..988)
                     /locus_tag="PLG49_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17769.1"
                     /translation="MIPKVIHYFWFGRGKKNDVIERCMDSWKVHLPDYEIKEWNEDNF
                     NVHINNFTLQAYQMKKWAFVSDYARLHILYHYGGIYLDTDLEITNSLDPFLDYEGFMG
                     FFSNFDISGGIIGAAKGNKFIKKLLTYYENKNFIDGNGKIDEKTNVEIITEISKKHFG
                     FVGENQTQQILDMTIFPKEYFTVRDVNVKNYAIHHYNASWLSHEEMNKHLKSYRKYYE
                     FTVKWLDNLINNKIDLTPYKNKCIAIFGKGYIGELLVSYLQNNNVPIRQIIDTKFAND
                     TYKDIPIVSSDTISDEVNLIIITPIHSFESIYLSLKEKSCADIISIENLLS"
     gene            complement(1027..2061)
                     /locus_tag="PLG49_10475"
     CDS             complement(1027..2061)
                     /locus_tag="PLG49_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ17770.1"
                     /translation="MRAVRIDGQTPGGKRNKLKDALPLDTPYMVQFFPIYACNFKCGY
                     CIHSVPVSQRRYITDKTAMDFNLYKKCIDGLSKFPNKVKMIRFAGTGEPLLHKDIAKM
                     VEYAVKMNVAEAVDIVTNGLLLEPDLSDKLIAAGLSKLRVSIQGVTSGKYEEISGKKV
                     GISKIVDNLSYFYNAKQKSENTTQLYIKIIDCALEEGDEEKFLNMFGDICDIIAIEHL
                     LPAVPQIEYKDKFNMDSDNLLTQNGNRILEAQVCPQPFYMMQINPEGNIVPCCAMETA
                     KVIGNVNDNDLYSLWVGEEFNKFRRNLLSLNKQIYPVCKSCESYKYSMFEEDFLDDAR
                     LKLLDLYVSR"
     gene            complement(2058..3080)
                     /locus_tag="PLG49_10480"
     CDS             complement(2058..3080)
                     /locus_tag="PLG49_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ17771.1"
                     /translation="MASEIKPIYGTERIKLSKVIPLKTPFSIFVFPTTYCNFKCVYCA
                     HSLGLDAMKKTYDFVPENMSMETYGKFIHQMKKFPDKLKMLCLMGQGEPLINKDIPVM
                     VKMAKDAEIAERVEIISNGSLLDKTMSDRLIEAGLDTLRISLQGLNSKKYKDICGANI
                     NFDDFMDNIRYFYAHKNNTNLFVKIMDVCLEEGEEEKFYRLFEDCSDRMYIEKMLPAY
                     DGVEITKNMKVEYDRYGRKHEKRNVCPLPFYMLGIFPNGDVEPCDTIYKPIVLGNINN
                     SELIDMWNGEKLREFWKLQLKGNRLQNDKCKVCCAPDDVSHPEDVLDDDVEEILKRLE
                     TMESNI"
     gene            complement(3114..>4156)
                     /locus_tag="PLG49_10485"
     CDS             complement(3114..>4156)
                     /locus_tag="PLG49_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOQ17772.1"
                     /translation="RLWKLMKAEFKPNYDTNRKKLADIIPLAAPFTVYIEQTRYCNFK
                     CFYCIHATRDKEDGEFRKLGFSVKHMDFEMYKTIVSQLKEFTEPIKRIVFSGLGEPLM
                     NPRLPEMVKLAVEAKIADRVEIITNGLLLTPETSRKLIDAGITNINISVQGVSKERYK
                     ETCGVEIDFDEYVKNLSYLYSIKGNTQIYIKAIDATLKSKEEEEKFFNIFGNICDKIY
                     IEHLIVMQQQMGELKKIVDGTKNFYNEELDLNRKVCAQSFYFLQIGCDYDTFPCPVPG
                     LPKSLSMGNIKDNTIKEIWNGEKRREHLRTMLSYQKDSIPECNNCTCFNAINNPLENL
                     DPDAPRLLKLFE"
BASE COUNT         1181 a          804 c          621 g         1550 t
ORIGIN      
        1 aatcttatga taacagattc tcaattgata taatatctgc gcaagatttc tcttttaacg
       61 ataaatatat gctttcaaag ctatgaatgg gcgtaataat tataagatta acttcatcac
      121 taattgtatc cgatgataca atggggatat ctttgtaggt gtcatttgca aactttgtat
      181 ctattatctg ccttattggc acattattat tttggagata gcttacgagc agttcgccaa
      241 tatatccctt gccaaatatg gcaatgcatt tatttttgta aggtgttaaa tctatcttgt
      301 tattaataag gttatcaagc catttaactg taaactcata atactttcta tagctcttta
      361 aatgtttatt catttcttca tgcgaaagcc aggaagcatt atagtgatga atggcataat
      421 tttttacatt aacatctctg actgtaaaat attctttggg gaaaatagtc atgtccagta
      481 tttgctgcgt ttggttttct cctacaaagc caaaatgttt tttggaaatc tcagtaataa
      541 tttcaacatt agttttttcg tcgatttttc catttccatc aataaaattt ttattttcat
      601 aataagttaa cagcttttta atgaatttat ttccttttgc tgctccaata attcctccgc
      661 taatatcaaa atttgaaaaa aaacccataa agccttcata atctaaaaac gggtctaatg
      721 aatttgttat ttccaaatct gtatctaaat agatacctcc ataatgatat aaaatatgta
      781 atctggcata gtcggataca aaagcccact ttttcatctg ataagcctgt aaagtaaaat
      841 tattgatatg cacattaaaa ttatcctcat tccattcttt aatttcgtaa tctggcaagt
      901 gaactttcca actgtccata catcgttcga ttacgtcatt ttttttgcct cttccaaacc
      961 aaaagtaatg aatgacttta ggtatcattg cagctttatc ctccttaatt gtttagaagt
     1021 ataaatttac cgagaaacat ataaatccaa caatttcagt ctggcatcgt ccaaaaaatc
     1081 ctcttcaaac attgagtact tataagattc acagcttttg cacacgggat atatttgttt
     1141 gtttagtgac agcaggtttc ttctgaattt gttgaattct tcacccaccc aaaggctgta
     1201 taaatcattg tcattaacgt ttcctattac tttggctgtt tccattgcac aacatggcac
     1261 aatatttcct tccggattaa tctgcatcat gtaaaatggc tgtgggcaaa cttgagcttc
     1321 taaaatacgg tttccgtttt gagttaaaag attatccgaa tccatgttaa atttgtcttt
     1381 gtactcaatt tgcgggacag ccggcaaaag atgttcaatt gcgataatat cgcaaatatc
     1441 tccgaacata tttaaaaatt tttcctcgtc tccttcttcc aatgcacaat cgattatttt
     1501 tatatataac tgtgtggtgt tttcgctttt ttgcttggca ttataaaaat aagacaaatt
     1561 atctacaatt tttgatatgc cgactttttt accggaaatc tcttcatatt ttccagatgt
     1621 aacaccttgt attgatactc taagctttga cagtccggca gcaattaatt tatcagacaa
     1681 atccggctcg agaagcaaac cgttagttac aatatcaact gcttctgcca cgttcatttt
     1741 aactgcgtat tcaaccatct tggcaatatc cttgtgcaat aaaggttctc cggtaccggc
     1801 aaaacgaatc attttaactt tattggggaa cttgcttaag ccatcaatac attttttata
     1861 taggttaaag tccattgcgg ttttatccgt tatataacgc ctttggctaa caggcaccga
     1921 atgaatacaa tagccgcatt taaaattgca ggcatatatc ggaaaaaatt gaaccatata
     1981 tggtgtatct aaaggaagtg catcctttaa tttattccgt tttccgcccg gagtttgtcc
     2041 gtcaattctt acagctctca tatattactc tccattgttt ccagtctttt taatatttct
     2101 tcaacatcat cgtccaatac atcttcagga tgggaaacat cgtccggagc acagcatact
     2161 ttgcatttat cgttctgtaa tctgttgcct tttaattgta atttccaaaa ttctcttaat
     2221 ttttctccgt tccacatgtc gattaattcg gagttgttta tattgcccag cactataggc
     2281 ttataaattg tgtcacaagg ttccacatcc ccgttgggga aaattcccaa catataaaac
     2341 ggcagaggac acacatttct tttttcatgt tttcttccgt atcggtcgta ctctaccttc
     2401 atatttttgg ttatttccac accatcgtat gccggcagca ttttttcgat atacattctg
     2461 tctgagcaat cttcaaaaag ccggtaaaac ttttcttctt cgccttcttc caggcatacg
     2521 tccataatct ttacaaataa atttgtgttg tttttatgtg cataaaaata tcgtatattg
     2581 tccataaaat catcgaaatt aatattagcg ccgcatatgt ctttatattt ttttgaattt
     2641 agaccttgca gtgagattct taaggtatcc aaaccggctt ctatcaatcg atctgacatt
     2701 gttttgtcaa ggagtgaacc gttactgata atttccactc tttctgcaat ttctgcatct
     2761 ttagccatct ttaccatgac aggaatgtct ttattaatca atggctctcc ttgtcccatt
     2821 aaacataaca ttttcagttt gtccggaaat tttttcattt ggtgaataaa ttttccgtat
     2881 gtctccattg acatgttttc agggacaaaa tcataagtct ttttcatggc gtccaatccc
     2941 aaagaatgag cacagtatac gcacttgaaa ttacaatagg ttgtcggaaa aacaaatatg
     3001 ctaaaaggtg tttttaaagg aatgacttta gacagtttta ttctttctgt tccataaatg
     3061 ggtttaattt ccgatgccat tttattctcc ttttaagtca aatttttaca cttttattca
     3121 aacagtttta aaagtctggg cgcatccggg tccaaattct ccagtggatt gttaatcgca
     3181 ttaaagcatg tacaattgtt gcactcaggt atgctgtctt tctgatagct gagcattgtt
     3241 cttaaatgtt ctcttctttt ttcaccgttc caaatttctt ttatagtatt atcctttata
     3301 tttcccatgg ataaactttt tggcaaaccc ggtacagggc aggggaaggt atcgtaatcg
     3361 catccaattt gtaagaaata gaaagattgg gcacatactt ttctgtttaa atccaattcc
     3421 tcattgtaaa agtttttagt gccgtccact atttttttga gttcacccat ttgctgctgc
     3481 atgacaatta aatgttctat ataaattttg tcgcatatat ttccaaaaat attgaaaaac
     3541 ttttcttctt cttccttgga ttttagtgta gcatctattg cttttatata tatttgtgta
     3601 ttacctttaa tactgtatag ataactaagg ttcttaacgt actcatcaaa gtcaatttct
     3661 actccgcaag tttctttgta tctctccttg cttacaccct ggacggatat gttaatgttt
     3721 gtgattcctg catcaatcag ttttcttgat gtttccggtg ttaataataa tccatttgta
     3781 attatttcca ctctatcggc tatttttgct tccacagcga gcttgaccat ttcaggcagc
     3841 ctgggattca ttagaggttc gcccagacct gaaaatacta ttctctttat tggttctgta
     3901 aattctttaa gctgactgac gattgttttg tacatttcaa aatccatatg ctttaccgaa
     3961 aatcccagct tgcgaaattc tccgtcctct ttatctcttg tggcatgtat acaataaaaa
     4021 catttgaaat tacagtacct ggtttgttct atatacaccg taaacggtgc agcaagaggt
     4081 ataatatcgg ccagcttctt tctgtttgtg tcataattcg gcttaaattc ggctttcatt
     4141 agtttccaca accttt
//
LOCUS       DAOSUD010000126         4062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9250_length_4062_cov_27.837285, whole genome shotgun sequence.
ACCESSION   DAOSUD010000126 DAOSUD010000000
VERSION     DAOSUD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_4062_cov_27.837285"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2815..3747)
                     /locus_tag="PLG49_10490"
     CDS             complement(2815..3747)
                     /locus_tag="PLG49_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17773.1"
                     /translation="MYNYLIFAVFLLILVLSGIVTTRSISKISSYLRLGHFAAGFIIM
                     AVTTGIPELVVGISSVMEGVPEVSLGNVLGSNVVNLSLIIGTAVLIAGGVSFEDDEIG
                     KWLVYPFFLAILPIFLTLDGVLSRVDGLFLIIIFLGYFLLVYRQSGFEEEEKVGAGEF
                     LLSLILFSAGLIILLISSRYLVHYAVLIALEMGVPVFFISIILISFGTSLPELVFETI
                     SILHGYKALAIGDLMGSTIVNSTLILGGVSVMGPILVTDLTDFQIVSIFLILLIFIFI
                     MFLKSKRGITRQKALLLIIIYVIFLFLSVITKSP"
BASE COUNT         1454 a          741 c          690 g         1177 t
ORIGIN      
        1 tggatgaacc caataacaat attactcaaa tactctacaa taccaaacat cagcctccta
       61 ttttatccaa catccaatat atactttttt tttatcagat aataaatgat aaggttagaa
      121 actggccaaa ggaaaaaatg ggataagtat cctatttcag agaagaggtt ctggtcaaag
      181 ccctcaaaag agctgaagaa gaaggatgag aaagtaggta agttgagata caagacttct
      241 ctccccaaat tccttcaaaa atcctaaact tcaaccaaac tgacttcaaa ctgaacaaaa
      301 acagactttc actcccgaaa gttggcaaag tcaaagtagc ccccatcgat agccatgaaa
      361 ggagtaataa tgaaaaagag taaaagcggg aaatgctttg caatctttca agttgaaccg
      421 atcatcactc accatctggc aaaatcgtcg gccttgacat cgataaaacc gactaccgta
      481 ttgactgtga tggtgtaaca ctcgaaaatc ctctattctt cgacaagaca ataaagaata
      541 taaaagaaag cgcgaaaaca atttgacaag gaagggttca aaagggagaa agcaaaaagg
      601 aaagtatgcc aagcttttta cgagaagctc gaaaaaaacc agagaaataa cttcctccac
      661 aaactctccc attaccacat tgacaaatac aacgtaatac tcctcgaaaa ttggatatca
      721 gaaaaaaggc caaaaggagc agtagtacgc tgaacaggca cgctcttaat tcttcctaga
      781 gtaaattttt cagcatactc ttctataagg ctgaatgagc cggtagacaa attgtagatt
      841 cccagttaaa agattactaa ggcttgtgcc atctgcagtt accttccaga aacggaatta
      901 aagaataaag gtttctcaca tccgagctac ggctggaaag ctgacaggga ttacaaagcc
      961 tctttaaact tgctgcatgt ggggctggga cattccccga acgtctgtgg agagaagacc
     1021 tttaccattt tctgatatag tagaagagtg atcggagggg caaatcctcc ccaaagaagc
     1081 aagaaacctt ccctcagtaa ggaagggtaa tttactcatc ctcatataac tatggtatgg
     1141 gctgctctct cacctaatct ttgatttttt ttatcagagt tgacgataat tatgattcct
     1201 acaagaccaa agaatagtaa atcaatgacc cttaatatat ttcgaacaag actttgtccc
     1261 ccatcttata gtttcaattg tattttcatc cacgaccttt attgaaagta accatttatc
     1321 ttttgacctt taccagcaag cccttttaag atgaagaaat agtcatatta taagatccgt
     1381 aatgaaacct aatatccaag catagtaata ttaagattat aggcgaggaa acatactatg
     1441 aaaccggcaa taaatgataa tatcgagaat ataatgaaat caataatgaa tgctacaatt
     1501 ccccgcgata aacttgcata ctcgaataat tttttttatc gcagattttg caaaaaattg
     1561 gcgccttcag agttgttagt tctgcaattt gggcgaatat tctcttccaa ccataaaaag
     1621 atcccccttt gatttttact cttatagtaa tggtgttatg attttttatt aaggtgaaag
     1681 tataaactca taaatatggt tgtgcctttt tggagaaatt ctgattctgc ggaaatcata
     1741 gtgtagagat cgcatatcag ataattaatc ttgaggggtg atcccataat atatccaaat
     1801 gaaaaggaat taataatgtt tatatttgtg gcgtttaaat agctggatga ttctaccagc
     1861 ttatttcatt tgcaattgga gtttaacaca ctcaacatca attaatggcc ttgctgcgtt
     1921 ttaagatgcg gctagaaatt taggtatggg aattcacgtc agctgttggt tttatgtctg
     1981 atttgtttaa aatgtttgat ttgaggagtg ctccatatta ccttgtagaa tttcttcttc
     2041 tggataaaaa tatccgaatc ttatcatgga tttatttgat gttaaaagat tttaaggcgt
     2101 cgattgaatg gaaccaatca taatgcccta tacaaaaatt atgcgcacaa aaaaaaaaca
     2161 agtgcggagg aaaaattaca aaagaaagaa gtcttaatga tagtagaagc aagggtaaat
     2221 gttatattgg ttcatattag ttggcttggc atgagatcag gaaaaaatta cattcccaca
     2281 gccaacatat catttacttt aaattcttat taaataaatt tccaattcac atcaaaatcc
     2341 aaaaaaataa aaccctaatt tcaattatat gataggccaa gacacaccct taaacttatc
     2401 cgtatcaaac tggcttttgg gaaatatcca caattttatt caaatatttt tttctaagat
     2461 ctgaacttat agtaactgct aaacccctca ccaacactgc aaaaaagaaa tcagaatgta
     2521 tataacctat aaaaaaaggc taaaaacatg caaaaattac aaattaccac agcattagaa
     2581 gggcctgata aacgttgaag atcttaaaaa aaagaggata taagatttag gtttaaggag
     2641 aagaatgcgc agagaatatc ctgtacctcg ataaaagtta ctatggtctg ggacgataaa
     2701 atttcttgca agctataatc tccccccccc tttttggagt gttgagtatt ccagaaaaat
     2761 gttataagta gaattctatg atttatagcg gctttttaca aggccatttc atctttaagg
     2821 agatttagtg attacactta aaaataagaa gataacataa atgattatta gcagtagggc
     2881 tttttggcgt gttatacccc tcttactttt tagaaacatt ataaaaataa aaatcagtaa
     2941 tattagaaag attgagacga tctgaaaatc tgttagatct gtgactagga ttggtcccat
     3001 aactgatacc ccgcccaaaa ttagggtaga gttgactatt gtgcttccca ttaggtcacc
     3061 tattgccagg gccttgtagc cgtgaagtat tgaaatggtc tcaaaaacaa gctctggaag
     3121 agaagtgcca aaagagatca atattatact tataaagaaa actggaaccc ccatctcaag
     3181 ggcaataagt accgcataat gaaccaaata tctagaactt atcaataaaa ttattaaacc
     3241 agctgaaaac aagattaaag ataatagaaa ttctcctgca cctacttttt cctcttcttc
     3301 aaaaccactt tgcctataaa ccagtaagaa gtatccaagg aatatgataa ttaaaaatag
     3361 accatcaaca cgagataata caccatccaa agtcaagaag ataggtagaa tggctaagaa
     3421 aaaggggtaa acaagccact tgccgatttc atcatcctca aaactaaccc ctccagcaat
     3481 taacactgca gtacctataa ttagggataa attaacaaca ttagaaccta aaacgtttcc
     3541 caaggaaact tcgggcacgc cctccataac agagcttata ccaaccacca attcaggtat
     3601 acccgttgta acagccatta taataaaacc agcagcaaaa tgacccaatc tgagatatga
     3661 agatatctta gaaatggatc ttgtagttac tatcccactt aaaacaagta taagcaaaaa
     3721 tacagcgaaa attaaataat tgtacatgga atggtgtata tgaattatta atatttatag
     3781 atcatggatg tatacttaat taggaagaat ccagtttttt cccttcactg ggagtttcag
     3841 agaaatattg gtaggatgaa ttttttgtgg ttttctcctt ttttaggttg gaggaatttt
     3901 caccgacctt ttggggggct gataatcttt ttttgggaag atctcactct aaatccatta
     3961 ggaagcagta aagttcttcc tccacattcc tttttttcct ttttgctttt ttgggagatt
     4021 tttttatctt tacgggttca acctcgggga aaccaccata ct
//
LOCUS       DAOSUD010000127         4010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9387_length_4010_cov_22.476865, whole genome shotgun sequence.
ACCESSION   DAOSUD010000127 DAOSUD010000000
VERSION     DAOSUD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9387_length_4010_cov_22.476865"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..664
                     /locus_tag="PLG49_10495"
     CDS             74..664
                     /locus_tag="PLG49_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOQ17774.1"
                     /translation="MKSLKGTQTEKNLMKAFMGECQARMRYTYYASTAKKEGYVQISN
                     IFTETADNEKEHAKRFYKFLAADPEIHDTTIEITGEYPVAFSSTKENLLAAAAGENEE
                     WEQMYPAFADIAEKEGFPEIATVFRNIASAEKRHETRFRKLAANIENGTVFKKDKTVL
                     WKCNNCGYVHEGPEAPTSCPACAHPQAHFEVFVETY"
     gene            complement(743..1570)
                     /locus_tag="PLG49_10500"
     CDS             complement(743..1570)
                     /locus_tag="PLG49_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOQ17775.1"
                     /translation="MDKIICMKNVSKEFKVLNRREGLKGSFLDLFSRDYKIVKAVNQV
                     NLEIEQGEIVGYLGPNGAGKSTTIKMMTGVLEPTSGEIIVDGRVPCKDRNRHAQNIGV
                     VFGQRSQLWWALPAIESFKILKDMYQVKGLGQFAFGVLVLSYAWKNLSLGLSLIIIIK
                     LIVSLITASLFMIAIMNIAAASCFWLINSGYIMVMAFRFKDYAKYPVSIFNSAFKLIF
                     TFVIPIAFIAYYPSLIFLRPDEIPLLTWLSPFIGILFFYLSYLIWMKGARSYSGTGS"
     gene            complement(1751..2254)
                     /locus_tag="PLG49_10505"
     CDS             complement(1751..2254)
                     /locus_tag="PLG49_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOQ17776.1"
                     /translation="MFEVGEYIVYGSNGVCKVEAVGKLKVPGMSKDKLYYTLSPYNAK
                     GSKIFTPVDNKKVVMRPVISKKEAIELIENIKNIDLLEIPDESRIEEEYKEALRKCDC
                     RELVKLIRTINKRISVRLKEGKKITAGDEKYLQMAEEYLLSELSIPLEMEKEQVKELI
                     LKEVGNF"
     gene            complement(2319..3161)
                     /locus_tag="PLG49_10510"
     CDS             complement(2319..3161)
                     /locus_tag="PLG49_10510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOQ17777.1"
                     /translation="MNLINKYFINSKGLRIFYRTDVPFDAKAIVIISHGYAEHSGHYI
                     ELCDFLVKNNFGVYAIDHRSHGQSEGKRGHIDSFFDFISDFDALVSMIKENHPDKAIF
                     TFGHSMGGLITFVYALVHPERNMKGQVFSGPALAAPWGLYKVPMTFYDIVGKTIPKAK
                     VYRIVKRRASRNYQYFKEFQNDPYDLKYSTIGFLKEFLYKGISWAQENAEKYDLPCLF
                     LQGTADKIIPYERVQSTFERISSKDKTLKLYEHCYHELIHEPEREEIMKDMLEWLEER
                     IKKN"
     gene            complement(3205..3924)
                     /locus_tag="PLG49_10515"
     CDS             complement(3205..3924)
                     /locus_tag="PLG49_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ17778.1"
                     /translation="MVNSYLENYVVFDLETTGCNPVKDYIIEIGVLKIVNGKIHSSFN
                     ELINPGIAIPNYITAITGITDGMVKGKPFIDEVLQDFLAFCQEDYILGHNILFDFKFI
                     KAKCHQKGYAFNKKALDTLEISRKHLRQLPSRSLGALCQYYGIELSHAHRAVHDAFAT
                     HQLFQCLKRDFFAMSPSSFEPKEMVWTPPKMEMITDKQKKYLQSLLRTYKINLNREVE
                     SLTKSEASRVIDHILSEYRYK"
BASE COUNT         1337 a          779 c          575 g         1319 t
ORIGIN      
        1 ttacttttca tttttgttat gctataataa aattataaaa aatgactaaa tgatataagg
       61 aggtatgctg cagatgaaat ctttaaaggg tacacaaacg gaaaaaaatt taatgaaggc
      121 atttatgggg gaatgccagg caagaatgcg ctatacatat tatgcatcaa cggcaaaaaa
      181 agaaggttat gtgcagattt caaacatatt tactgaaaca gccgataatg aaaaagaaca
      241 tgcaaagaga ttctataagt ttttagctgc tgatcccgaa attcatgata ccacaataga
      301 aattaccggc gaatatcctg ttgcttttag cagcacaaaa gaaaatttac tggcagcggc
      361 ggctggagaa aacgaagaat gggaacaaat gtatccggca tttgcagata tagctgaaaa
      421 agaaggtttc ccggaaatcg ctacggtatt cagaaatatt gcttctgctg aaaaaagaca
      481 tgaaacccgt tttagaaaat tagctgctaa catagaaaat ggtacagtat ttaaaaaaga
      541 taaaacggta ttatggaaat gcaataactg cggatatgtt catgaaggac cagaagcacc
      601 aacatcctgt cctgcttgtg cccatcctca ggcgcatttt gaggtatttg ttgaaaccta
      661 ttaatataac agatgagctg ttgcactcat tcataaacgt gcaacagctt tttttatcta
      721 ttatctgcag caatttttta ttttatgaac cggttccgct ataggacctt gcccccttca
      781 tccaaatcag gtatgaaaga tagaaaaata atattcctat aaagggagac aaccaggtta
      841 aaagtggtat ttcatcaggt cttaaaaaga tcagactggg atagtaagca ataaaagcaa
      901 taggaatgac aaaagtaaaa atcaacttaa aagcagaatt aaaaatactg acaggatatt
      961 tagcgtagtc tttaaagcga aaagccatca ccataatata acctgaatta ataagccaga
     1021 agcaacttgc tgctgcgata ttcataatgg caatcataaa taaagatgcc gtaataaggc
     1081 ttacgataag ctttataata atgattaaag acagacccag acttaaattt ttccaggcat
     1141 agcttaacac caaaacacca aatgcaaact gacccaaacc cttaacctga tacatatctt
     1201 ttaaaatttt aaaagattca atggctggca aggcccacca caactgagaa cgctgtccaa
     1261 atacaacacc tatattttga gcatgccggt ttctgtcttt acagggaacc ctgccatcta
     1321 caataatttc tccggatgtg ggttccaaaa cgcccgtcat catttttatg gtcgtacttt
     1381 ttcctgcccc gttaggtccc aaataaccta caatttcccc ttgctctatt tccaaattca
     1441 cttgattaac agcctttaca attttatagt cccttgagaa caaatccaaa aaactgccct
     1501 taagaccttc tcttctattt aatactttaa attctttact cacatttttc atgcaaataa
     1561 tcttatccat tgctttcacc ttcaatcttt attggcccaa ataatataat tacttatctt
     1621 acaatctatt taataagttg tcaatattaa gaaaataatt aaaatattaa taaaaaaata
     1681 gagaaataaa ccgtgtttat ttctccataa atcctttctt attaagcgat tatccaagaa
     1741 acctgttctt ttaaaaattt cctacctctt ttaaaatcaa ttctttcact tgttcctttt
     1801 ccatttctaa gggaatggac aattccgata ataaatattc ctcagccatt tgcagatatt
     1861 tttcatcgcc agccgttatt ttttttcctt ccttcaaacg gacactgata cgcttattaa
     1921 ttgtacggat taacttaacc aattccctgc aatcacattt tcttaaagct tccttatact
     1981 cttcttcaat ccggctttca tcaggaattt ctaataaatc aatattctta atgttttcaa
     2041 tcagttcaat ggcttctttt ttgctaataa caggtctcat aaccactttt ttattgtcta
     2101 ctggtgtaaa tatcttactt cctttagcat tataagggga taaagtataa tacaatttgt
     2161 cctttgacat gccgggtacc tttaattttc caactgcttc aaccttacaa acgccattac
     2221 tgccgtaaac gatatattct cctacttcga acatgaaaag cctcctttct attcttataa
     2281 acatttgcta taggattcat gaacactttt tacctttgtt aattcttttt aattctttct
     2341 tccagccatt ccaacatatc cttcataatt tcttctcttt cgggctcgtg gatcaattca
     2401 tgataacaat gttcatagag ctttaaggtt ttgtctttcg aagaaattct ttcaaatgta
     2461 ctttgaactc tttcataagg aatgatttta tccgctgtcc cttgtaagaa aagacaaggt
     2521 aaatcatatt tttctgcatt ttcctgtgcc caactgatac ctttatataa aaattctttt
     2581 aaaaaaccta tggtcgagta ttttaaatca taagggtcat tttgaaattc cttaaaatac
     2641 tgataattcc ttgaagctct tctcttaaca atccggtaaa cttttgcttt tggaatggtt
     2701 ttacccacaa tatcataaaa ggtcatgggt accttgtata agccccaggg cgctgctaaa
     2761 gccggaccag aaaatacctg acctttcata tttctttcag gatgcacaag ggcataaaca
     2821 aaagtaatta agcctcccat actgtgacca aaggtaaaaa ttgctttatc aggatgattt
     2881 tctttaatca ttgaaacaag tgcatcaaaa tccgagatga aatcaaaaaa tgaatcaata
     2941 tgacctcttt tgccttcaga ctgaccatga cttctatggt caatagcata gacaccaaag
     3001 ttatttttaa ctaaaaaatc acacaattct atgtagtgac cagaatgctc tgcataaccg
     3061 tgactgataa taacaatcgc ttttgcatca aaggggacat ccgttctgta aaaaatccgc
     3121 aaacctttag aattaataaa gtatttatta attaaattca taacgctctc cttttcgaaa
     3181 tttaacatat gccttttttc aagcctactt atatctatat tcagacaata tatgatctat
     3241 aaccctgctg gcttctgatt ttgttaaact ttccacctct ctgttaagat ttatcttata
     3301 agtcctcaac aaactttgta aatatttctt ttgtttatct gttatcattt ccattttagg
     3361 gggagtccat accatctcct taggttcaaa ggacgaaggg ctcatagcaa aaaagtctct
     3421 ttttaggcat tgaaataatt gatgcgttgc aaaagcgtca tgaactgccc tgtgggcatg
     3481 ggataattct attccatagt attgacaaag agctcccagg cttctgctgg gaagctgcct
     3541 taaatgtttt cttgaaattt ctaaagtatc taaggctttc ttattaaaag catatccttt
     3601 ttgatgacat ttggctttga taaatttaaa gtcaaataaa atattatgac ctaaaatata
     3661 atcctcctga caaaaggcca aaaaatcctg aagcacttca tcaataaacg gcttgccttt
     3721 taccatacca tctgttatac ccgttattgc cgtaatataa ttaggaattg ctataccagg
     3781 attaatcagt tcattgaaac tggaatggat ttttccattg acaatcttca ggacacctat
     3841 ttcaatgata taatctttaa ctggattgca tccagtagtt tctaaatcaa atacaacata
     3901 attttccaaa tagctgttaa ccattgctat acctttctga atttataata aaagcttata
     3961 tatattatat catttcctcg actatcattc tagtaaagtc tttagaaaac
//
LOCUS       DAOSUD010000128         3976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9471_length_3976_cov_22.395817, whole genome shotgun sequence.
ACCESSION   DAOSUD010000128 DAOSUD010000000
VERSION     DAOSUD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9471_length_3976_cov_22.395817"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..559
                     /locus_tag="PLG49_10520"
     CDS             248..559
                     /locus_tag="PLG49_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17779.1"
                     /translation="MKKVQVENSDNGADIYVYIENNSSQNITVQVKEMSINGYMIDPI
                     FSCDVLAGKKAYDIITFLENELEKNDISSIDEIELSFDIFDTLTFETILESDAVVINF
                     K"
     gene            739..2256
                     /locus_tag="PLG49_10525"
     CDS             739..2256
                     /locus_tag="PLG49_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17780.1"
                     /translation="MGLESIYDVISRSINSEGKLDLNFMLPFKKPSPTEMRFIPGAKD
                     GIGLYHFNLRTPKSAANKIFKLLKSDWKKGDSDSQEKVAEILNRYGSWTVIDYVLHLI
                     RDKQKEIDPKNIFYYACKLAFESTNEELVKLGICLFGLLDLSNEEEIMNRLFILALYE
                     EFTLYVLATISNSPKGNDIVFKIAQKVDGWGKIHAVEYLEPATDEIREWILRKGCANA
                     VMDAYLGLECAIKGDLINALKRDSFDEELFEGISVIIDALLDEGPVDGISVYEHTEEA
                     LKLYLRIATEHAFTIIHLWRLLNLQAWLENATISNRDELKKICDNIVNREAWKEMIME
                     ILNKPDDELLFYAINAGLRLNMDIIEFVFRAVKKNPVEHFGYLHILYKNPEYANELTK
                     IYEELLPLDAMAAGMGNFLFPENYAKEHHCLEFILQELKNYPEMGEKLVETGLKSPVV
                     RERIAACKVIEEWSKRLNQNLDTISPGLYAILKEIARIEVDKDSRQLMKKLLRTK"
     gene            complement(2543..3004)
                     /locus_tag="PLG49_10530"
     CDS             complement(2543..3004)
                     /locus_tag="PLG49_10530"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOQ17781.1"
                     /translation="MKTLENIFVITLFLIYLGLWKIKQLKQIKNTGVDPAVIGKSTSS
                     IQKYFYQCTNILTLYAVLMIVLHSSQFQLGSLFSRITVLDQFLFDLIGFFTGLMGLSL
                     CFYAQVKMGNSWRVGVDTNAKTDLITTGLYKYIRNPTYLGLFLLNMGLWLI"
     gene            complement(3287..3727)
                     /locus_tag="PLG49_10535"
     CDS             complement(3287..3727)
                     /locus_tag="PLG49_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17782.1"
                     /translation="MYIYPNVIEGTAFTTMELIEYVDKGPIPNRNGIYELLAFIKLKV
                     NDQVISDNDSPFNKILRRLCRIFTDISNYSYLAKLEPGDTCEIPGKEGEENICLIFDE
                     YKKADHAFIIHGHLFGFLVCIEIFRSEMEYAVAARFAYLLHPTL"
BASE COUNT         1429 a          557 c          717 g         1273 t
ORIGIN      
        1 tttatatttt aaggcgttcg gctttttgtg tctacactaa tatactaaca ccagaggcct
       61 ggatatagca ggaatagaag taataaaaga tatagaatta agatttgaag tatataacac
      121 ggatacatgg gatacttatt ttgaatcaga tccgattgtt ataacaacta cagcggttcc
      181 atcttatgtt caaaaatacg atgacagtgg ttttgtagca gtcgatcaaa atggtataaa
      241 aattgtaatg aaaaaggtgc aggttgaaaa cagtgataat ggtgcagata tttatgtata
      301 tatagagaat aacagcagtc aaaatattac agtacaagta aaagaaatgt ccattaatgg
      361 ttatatgatc gatccgatat tctcatgtga tgttttagct ggtaagaagg catatgacat
      421 aataactttt cttgaaaacg aattagaaaa aaatgatata agctctatag atgaaataga
      481 attgtcattt gatatatttg atacgcttac atttgaaacg attttagaat ccgatgcagt
      541 tgttattaat tttaaatgaa gcaaggggac ggttccctgt ttgattttga gtttaaagaa
      601 taacagatta ttgtaaggaa gcacaaggac tgccttgtgc tttcttttca ggatttatgc
      661 taatggaaaa aatttaaata tcttttgtaa gggaggaatc ttcttgttct tagattaaat
      721 tgaaagtgag ggataaccat gggtttagaa agtatatacg atgtcatcag tagaagtata
      781 aattccgaag ggaaactaga tttaaatttt atgcttccat ttaaaaaacc ttcgccaact
      841 gaaatgcggt ttattcctgg tgcaaaagat ggaattggtt tatatcattt taatttaaga
      901 acccccaagt cagctgcaaa taaaattttt aaacttttaa aaagtgattg gaaaaagggc
      961 gacagtgact cacaggaaaa agttgctgaa atccttaata ggtatggaag ttggacagtt
     1021 atagattacg ttcttcattt aattcgggat aaacaaaaag agattgatcc caaaaatata
     1081 ttttattatg cctgcaagct tgctttcgaa tcaacaaatg aggaattggt taaattagga
     1141 atttgcttat ttggtttact tgatttgagt aacgaagaag aaattatgaa tagacttttt
     1201 atacttgcat tatatgaaga gttcaccctt tatgttcttg ccacaatctc aaattcaccc
     1261 aaagggaatg atattgtctt taaaattgca caaaaggttg atggatgggg taaaattcat
     1321 gcagtagagt acttagaacc agcaacagat gaaatacgag aatggatttt acgcaaaggc
     1381 tgtgccaatg cagttatgga tgcttatctt ggactggaat gtgccatcaa aggggatttg
     1441 ataaatgctc taaaacggga ttcttttgat gaggagcttt ttgaaggcat tagtgtgata
     1501 atagatgccc tattagatga aggacccgtt gatggtatca gcgtttatga gcacactgag
     1561 gaagcattaa agctttattt gaggatagct acagaacatg cttttaccat aatacatcta
     1621 tggcggctcc ttaatttgca agcttggtta gaaaacgcca caataagtaa cagagatgaa
     1681 ttgaaaaaaa tctgcgataa tattgtaaat agagaagcat ggaaagaaat gatcatggaa
     1741 attcttaata aacctgatga tgaactgtta ttttatgcaa tcaatgcagg tttgagactg
     1801 aatatggata ttatcgaatt cgtttttaga gcggtaaaga aaaatccagt tgaacatttt
     1861 ggttaccttc atattcttta taaaaatcca gaatatgcaa atgaattaac gaaaatatat
     1921 gaagaacttt taccactgga tgccatggca gcaggaatgg gcaattttct ttttccagag
     1981 aactatgcaa aggagcatca ttgtctggag tttatacttc aggaacttaa aaattatcct
     2041 gaaatgggcg aaaagttggt agagacagga ttaaaatcac ctgtggtaag agaaaggatt
     2101 gctgcatgta aagtgataga ggaatggagt aagagactaa atcaaaattt agatactatc
     2161 tcacctggct tgtatgctat tctaaaagaa atcgcacgta ttgaagtgga taaggatagc
     2221 aggcaattga tgaaaaaatt acttcgaact aaatgaaaca aggaaacaca gagacgattc
     2281 cttgtttcat tttttgttta aagaataaac aggttattat aaaactgcca aatgtttttc
     2341 attagaagga aaagttcaaa aattcagaaa aaatcaaaag aactgccttc taatatataa
     2401 aaggaatata tcttttcgtt ctttttttat attctatgta ctttgagcca tgcattgagc
     2461 ttaagtaatc ctcttcacag cgcacttgga tatccagtaa tagtacaaaa actaaattaa
     2521 acagaaatac agtccaggtt ggtcaaataa gccaaagacc catatttaat aaaaataatc
     2581 ctagatatgt tggatttcta atgtatttat ataagccggt cgtaatcaaa tcagtttttg
     2641 cattcgtgtc aactccaact ctccatgagt tgcccatttt tacctgagcg taaaaacata
     2701 gagacaaacc cataagacca gtaaaaaaac caatcagatc aaatagaaat tggtctaaaa
     2761 cggtaattct gctaaacaaa gaacccagtt ggaactggct tgaatgtaaa acaatcatta
     2821 aaacggcata gagggttaga atatttgtgc actgataaaa atatttttga atacttgatg
     2881 tggattttcc tataacagca gggtctacac cagtattctt tatttgtttt agctgtttaa
     2941 ttttccataa ccctaaatag ataagaaata atgtgatgac aaatatgttt tctagagttt
     3001 tcataatatc tcctcctgat aatatttttt tggcataaag cttttcatct tgtatgagaa
     3061 aaggacatta tactttttta aagtaaagaa cgaacatatg aacaattatt catatattac
     3121 ataatattat gtccagttca aaatgtctag aaggtaggaa ttcagaaagg aacaattaat
     3181 aagataaatc agataaaaaa agtaatgttt taaaagagga taaaaggagg gtattaattg
     3241 atgtaaaaga ggataatatc tggctggtat caaactggaa gttaacttat aaggttggat
     3301 gaagcaaata tgcaaagcga gcggctacag catattccat ttcacttcta aaaatctcta
     3361 tacatactaa aaagccaaat aggtgcccat ggataataaa tgcatggtca gcctttttgt
     3421 attcatcaaa aataaggcaa atattttctt ctccttcttt accaggtatt tcacatgtgt
     3481 cacctggttc taactttgct agatatgagt agttactgat atctgtaaag attctgcaca
     3541 agcgtctcaa tattttatta aaaggagaat cattatcaga tattacctga tcattgactt
     3601 taagttttat aaaagctaaa agctcatata tgccatttct gttaggaatg ggacctttat
     3661 ctacatattc aattaattcc attgtcgtaa atgcagtacc ctcgatgacg ttaggataga
     3721 tatacatatc tgctaaaccg ccaagacaaa atggaatgag tgaatgagaa actaaatgga
     3781 atggttatcc taatattctg tcaatatcct tttctttctt ttcaaagaat tcatttaatt
     3841 tttcctcaga aatgttttga ttagatcttt cattataagc actcattttt tatcctcctg
     3901 tatttatatt tagaaagtta tccgtataat tccctattta tgataattat aggtattagc
     3961 tatctattaa attcaa
//
LOCUS       DAOSUD010000129         3943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9555_length_3943_cov_19.588992, whole genome shotgun sequence.
ACCESSION   DAOSUD010000129 DAOSUD010000000
VERSION     DAOSUD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_3943_cov_19.588992"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..733)
                     /locus_tag="PLG49_10540"
     CDS             complement(44..733)
                     /locus_tag="PLG49_10540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17783.1"
                     /translation="MNIGIFTDTYYPQINGVVTSIRTLEKELNKRGHKVYIFTTSNPN
                     SKRALPRVFRLPSMPFIFLPSHRVGVLYSPKAVRIVKQLDLDIIHTQTEFSLGLFGKM
                     LSKQLGIPIVHTYHTMYEDYAHYISKGKLTEFTPKMARSLSRLFCNRCDAVIAPTNKV
                     KELLLEYGVRKPIEVIPTGINFEPFKKENYDQAEIIELRKQFGIQESDPVVLFVGRVA
                     KEKSIDVVIPI"
     gene            complement(751..1386)
                     /locus_tag="PLG49_10545"
     CDS             complement(751..1386)
                     /locus_tag="PLG49_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17784.1"
                     /translation="MKIPLFFSLFILFVVFFNIKLRKASKEIENSNANFWKRERESMF
                     ARKKSLDHLNYISVSLNDLPMLKEEELFSDAEKKAYSHQKTALSLASKPMLNLSGISN
                     TDLKLQYGPANLENLMSYEQNYENLIKSLLNWGRYLKEANRTSDAIAVLEKAVSIGSD
                     LSQNYILLSDLYRETQQIDKLLSLKNEVENRLKQNAMQKKVLQYIDNILNM"
     gene            1587..1970
                     /locus_tag="PLG49_10550"
     CDS             1587..1970
                     /locus_tag="PLG49_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ17785.1"
                     /translation="MKKKEFLYWIPSIIWMIIIFILSSMTGSDLQRTFPFFSDFNWGH
                     FVAYFVLAITYYWALRRKVKHPKIFFVIIFLSVLYGITDEFHQYFTPSRVPDVNDLLA
                     DAIGASLAAILLSIWQKKRNTRLKL"
     gene            1987..>3943
                     /locus_tag="PLG49_10555"
     CDS             1987..>3943
                     /locus_tag="PLG49_10555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ17786.1"
                     /translation="MFEADVIEEFIKIRDRIIEQAYGHLNENQRKAVLTGEGPQMIIA
                     GPGSGKTHVIMHRLHYLISYGMVYNTRKIPEGITKEDIDILKGDIHHPRSKELLNYYA
                     IDPRSILVITFTKAAAEEMKSRFFNMKDVNGTRIKSILFGTFHSVFFRILRNAYGYHV
                     DQVLKEDEKRQVLKKIAEELEIEYQDEQEFLDDLENEIGLIKNDLIELSYYNSMTLPS
                     EQFRKIVSYYEQYKSQHQKIDFDDMLYDCYILLREHKNILSFWSNRYKYILIDEFQDI
                     NKVQYETIKLLSAPNNHLFIVGDDDQSIYKFRGARPEFLLHFPQDFKDSGRVTLDINY
                     RSTRKIVDISNSVISKNVHRYPKEIRTINPKGDEPVFIQSEDSEEEALHIAEWILNWR
                     KKGLSYRDIAVIFRTNLQARALVDIMLDLNIPFYLRDEIPNVYEHWVAKDLLAYIKLS
                     KDIKDNESLERIINKPKRYISKGVIFEACKKGGILFENIYKTPQLQTWQVNRLEELKF
                     HLDCMKNKKPGEAIVYIRKHIGYDDYLAEYAQYKNISVKGLKELLDEIQEAAKHYDSF
                     EEWMYHIQNVGEEIKEGKRRIIGEVDAVTLSTMHGAKGLEFEAVCIAGAVEGVIPHNK
                     STSPAELEEERRLFYVGITRAKKYLAIS"
BASE COUNT         1334 a          594 c          723 g         1292 t
ORIGIN      
        1 tatgctaaca tattcgcatt ttttctccaa acctattagg gggctaaata ggaatcacta
       61 catcaatact tttctctttt gccactcttc caacaaacag aaccactgga tcagattctt
      121 gtattccaaa ttgctttctg agttctataa tttctgcttg atcatagttt tcttttttga
      181 aaggttcaaa gttgatacca gtggggatta cttcaatggg ttttctcacc ccgtattcca
      241 gtaagagctc ttttacttta tttgttggag caattactgc atcgcatcgg ttgcagaaca
      301 gacgacttaa agaacgtgcc atttttggag taaactccgt gagctttccc tttgaaatat
      361 aatgcgcata atcttcatac atagtatggt aagtatgtac aataggaatt cccagctgtt
      421 tagaaagcat tttaccaaaa agccctaatg aaaactctgt ttgggtatga ataatatcta
      481 aatcaagctg ttttacaatt ctcacagctt taggggaata taatacaccc accctgtgag
      541 aaggaaggaa aataaaaggc atgctgggaa gtctgaaaac tctgggtaga gctctcttag
      601 aattaggatt tgaagtagta aaaatatata ccttgtgacc ccgtttattc agttcttttt
      661 ctaaagtacg aatagaggtc acaacgccgt taatctgtgg ataataagta tcagtaaaaa
      721 ttccaatatt cataagaacg ctcctattct ttacatattt aatatgttgt caatatattg
      781 gagaactttt ttttgcatag cattttgctt cagacggttt tccacttcat tttttaaact
      841 taataattta tctatttgct gtgtttcacg gtataaatca gatagcaaaa tataattttg
      901 gcttaaatct gagccaattg aaacagcttt ttcaagaact gctatagcat cgcttgttcg
      961 atttgcttct tttaaatatc tcccccaatt cagtaatgat tttataagat tttcatagtt
     1021 ttgttcataa ctcatcaagt tttccagatt ggcaggacca tattgcagtt tcaaatccgt
     1081 attgcttatg ccgcttaagt taagcattgg tttagaagcc aaagacaaag cggttttttg
     1141 atggctgtaa gccttttttt cggcatcaga aaataattct tcctctttaa gcataggaag
     1201 atcatttaaa gataccgaaa tatagttcaa atgatctaag gattttttac gtgcaaacat
     1261 ggactcccgt tctcttttcc agaagtttgc atttgaattt tcgatttctt tgcttgcttt
     1321 acgtaatttt atattaaaaa atacgacaaa aagaataaaa agactaaaaa acaatggtat
     1381 tttcataaaa accttcctta cataattatt ttctgcgctt attatagtat atattttcca
     1441 taaagtctat gacataagaa ttataaaata tattttttac aaatagtgat agacataggc
     1501 tgaaaatctc cttattccgt aaaatcatat atttttatac aaagtgcgtt tctcctatgg
     1561 tataataacc ccaaggagag atgatgatga agaaaaaaga atttttatac tggattccgt
     1621 ccataatttg gatgattatt atttttattt tgtcctcaat gactggaagt gatttacaaa
     1681 gaacatttcc ttttttttct gactttaatt gggggcattt tgtagcttat tttgttttgg
     1741 ctattactta ttattgggca ttgcgcagga aagttaaaca tccgaaaata ttttttgtga
     1801 ttatcttttt atctgttttg tatggcatta ctgacgaatt tcaccagtat tttactcctt
     1861 ccagggtacc ggatgtcaac gatttactgg cagatgccat tggggcttct ttagccgcaa
     1921 ttttattaag tatatggcag aagaaaagaa atacaagact aaaattatag atcagtagga
     1981 gaaactatgt ttgaagcaga tgtgattgaa gaatttatta agatcagaga tagaattata
     2041 gagcaagcct atggacattt aaatgaaaat caaagaaaag cagtgttaac tggagaaggc
     2101 cctcagatga tcattgcagg tcctggctcc gggaaaaccc atgttattat gcacagatta
     2161 cattatctta tatcctatgg gatggtttat aatacacgta aaataccaga aggcattact
     2221 aaagaagata ttgatatact gaaaggcgat atccatcacc ccagatcaaa agaattgtta
     2281 aattattatg ctatagatcc cagaagtatt ttggttatta cttttacaaa agctgctgca
     2341 gaagaaatga agtcaagatt ttttaatatg aaagatgtaa atggtactcg aatcaaaagt
     2401 attttatttg gaacgtttca ttctgttttt tttagaattc taagaaatgc ttacgggtat
     2461 cacgtagacc aagtacttaa agaagatgaa aagcgccagg ttttaaagaa gatagcagag
     2521 gaacttgaaa tagaatatca ggatgagcag gagtttctgg atgatttaga gaatgaaatt
     2581 ggtcttatta aaaacgattt aatcgagctg tcttactata attctatgac tttaccatct
     2641 gagcaattta gaaaaatagt gtcttattat gagcaatata aatcccagca tcaaaaaatt
     2701 gattttgacg atatgcttta tgattgctat atacttcttc gggagcataa aaatattctt
     2761 tctttttgga gcaatcgata taaatatata ttaatcgatg aatttcagga tattaataaa
     2821 gttcagtacg aaacgatcaa acttttaagt gctcctaata atcatctgtt cattgtagga
     2881 gatgatgatc aaagcattta taaattcaga ggagcaagac cagagttttt gcttcatttt
     2941 ccacaggatt tcaaggactc gggaagagta actttagata taaattatcg ctctacgagg
     3001 aaaattgttg atataagcaa ttctgtaatc agtaaaaatg tgcatagata tccaaaagaa
     3061 atcagaacga ttaacccaaa gggcgatgaa ccagttttta ttcaatcgga agatagtgaa
     3121 gaagaagctc ttcacattgc agaatggatt cttaactgga ggaaaaaagg cctgtcttat
     3181 cgggatattg cagtaatctt cagaaccaac cttcaggcga gagctttggt agatattatg
     3241 ctggatttaa atattccttt ttatcttagg gatgaaattc caaatgttta tgaacattgg
     3301 gtggcaaaag accttttagc gtatatcaaa ctatctaaag atatcaagga taatgaaagt
     3361 ttagaaagga ttattaataa accaaagcga tatatcagta aaggtgtaat ttttgaagcg
     3421 tgtaaaaagg gtggaattct ctttgagaat atatataaaa cacctcagtt gcaaacatgg
     3481 caggtaaatc gactggaaga acttaaattt catttggatt gcatgaaaaa taaaaagcca
     3541 ggagaagcta ttgtctatat caggaaacat ataggatatg atgattacct ggcggagtat
     3601 gctcaatata aaaacataag cgttaaaggt ttaaaggaat tattggatga aattcaagag
     3661 gcggccaagc attatgatag tttcgaggaa tggatgtacc atattcaaaa tgtaggagaa
     3721 gagatcaaag aaggcaaacg cagaattata ggagaagtgg atgctgttac attatctacc
     3781 atgcatggag ccaaaggtct tgaatttgaa gcggtttgca ttgcaggagc tgtagaaggg
     3841 gttattcctc ataataaaag tacttcccca gctgaattgg aagaagagag aaggctgttt
     3901 tatgtgggga tcacaagagc aaagaaatac ttggcaatat caa
//
LOCUS       DAOSUD010000130         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_9767_length_3870_cov_24.744168, whole genome shotgun sequence.
ACCESSION   DAOSUD010000130 DAOSUD010000000
VERSION     DAOSUD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_3870_cov_24.744168"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PLG49_10560"
     CDS             <1..1198
                     /locus_tag="PLG49_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ17787.1"
                     /translation="LGMFDECEYDKISYLENDSKEHHEIALMAARKSVVLLKNDGILP
                     LDKNKIKTIAVIGPNANSREILKANYCGTASKYITLLEGIQNSVSEETRVLYSEGCHL
                     FKDRVEPLALPNDRISEAKAIARLSDVVILCLGLDANLEGEEGDTGNSYAAGDKLDLN
                     LPGLQQMLLEEIIKIGKPVILLLSSGSALAIDYAHEHCNAILQTWYPGALGGQAVAEL
                     LFGKYSPSGKLPITFYSQKNKLPDFSDYSMKNRTYRYIEEEPLYPFGYGLTYGKVELS
                     NLKVDKVQIRENDIITISVDVENQSDWELEEVIQVYAKVTGTEYEVLNHKLCAFKRIK
                     LEKAEKRTVSLELKAENIKVVDYHGNRIFDGDKVILFVGTSQPDKRSKALTGQEVCQI
                     ELPLLK"
     gene            complement(1225..2511)
                     /gene="serS"
                     /locus_tag="PLG49_10565"
     CDS             complement(1225..2511)
                     /gene="serS"
                     /locus_tag="PLG49_10565"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ17788.1"
                     /translation="MLDLKFIRENPEIVKQNIQKKFQDHKLPLVDEVIALDLERRTSQ
                     QEADNLRANRKKISKEIGNLMAQGKKEEAEELKKQVTANAERLAELEEKERVLTEKIQ
                     NILMVIPNIIDPSVPIGKDDSENVELQRYGEPVIPDFEIPYHIDIMESFSGIDLDSAR
                     RVSGNGFYYLTGDIARLHSAVLTYARDFMIDRGFTYCLPPFMIRSNVVTGVMSFEEMD
                     AMMYKIEGEDLYLIGTSEHSMIGKYIDTIIDEETLPHTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVICKPEDSMDWFNKLWQNTVDIFRSLDIPVRTIECCSGDLADLK
                     VKSIDVEAWSPRQQKYFEVGSCSNLGDAQARRLKIRVYGKEGKYYAHTLNNTVIASPR
                     MLIAFLENNLNKDGSVNIPKVLQPYMGNKTKLIPGK"
     misc_binding    complement(2581..2779)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2963..3835
                     /locus_tag="PLG49_10570"
     CDS             2963..3835
                     /locus_tag="PLG49_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ17789.1"
                     /translation="MRIQSLSVCVPNKGCVNNCKFCVSHMRNDDYKNMMDENLPFYDL
                     YEKDYIRRLEFARDNGCNTVMLTGNAEPQQNRAFLQRFGTMNSNLSKPFRWIEMQTTG
                     VMIDEPYLRFLRNHVGVSTLSLSVSSFDNEQNRDYNGTSLKNKVDLIEFCNSVKKYDF
                     NLRISINLTDAFNDWSAARIFKYCKEVFNADQITFRVLYRSGSDTAQDRWIAEHKAKD
                     ELIDEIRNYILNKGRKLEKLEFGAIKWSVDEMSTVLDEDCMSEEAKESVKYLILRPNC
                     KLYSKWDDKGSLVF"
BASE COUNT         1239 a          617 c          739 g         1275 t
ORIGIN      
        1 gttaggcatg tttgatgaat gtgagtacga taagatttct tatctggaaa atgacagtaa
       61 agagcatcac gaaatcgctc ttatggcagc aagaaaatcc gttgttctct taaaaaatga
      121 tggaatatta ccccttgata aaaataaaat taaaacgatt gcagttattg gtcctaatgc
      181 caatagccgt gaaatcctga aagcaaatta ttgtggcacc gcctcaaaat atataactct
      241 tttggaaggc attcaaaata gtgtcagtga ggaaacacgg gtactttatt cagaaggttg
      301 ccacttgttt aaagacagag tagagccttt ggcacttcca aatgacagaa tcagtgaagc
      361 aaaagcaatt gcacgtttat ctgatgtagt gattttgtgt ctgggattag atgctaattt
      421 agaaggagaa gaaggcgata ctggaaacag ctatgcagca ggtgataaat tagatcttaa
      481 tttaccaggc ttacaacaaa tgcttttaga agaaataatt aagattggta aaccagtgat
      541 attgctttta agttcaggta gcgctttagc cattgattat gcccatgaac attgcaatgc
      601 gattttacag acatggtatc ctggtgctct tggtgggcag gcagtagcag aattattatt
      661 tggcaaatat tcaccgtcag gaaaacttcc gattaccttt tatagtcaga aaaataagtt
      721 gcctgatttt agcgattatt ctatgaaaaa cagaacttac aggtatattg aagaagagcc
      781 cctttatcct tttggttatg gacttactta tggaaaagtt gagctttcca atcttaaggt
      841 ggataaagtt caaataagag aaaatgacat tattaccata tctgtagacg tagaaaacca
      901 atctgactgg gagcttgaag aagttattca agtatacgca aaagtaacag gaacggaata
      961 tgaggtttta aatcataagt tatgtgcatt taagagaatt aagcttgaaa aagctgaaaa
     1021 aagaacagtt tcattagagc ttaaggcaga aaatataaaa gtggtagatt atcatggtaa
     1081 tcggatattt gatggagata aagtgatttt atttgttgga accagtcagc cggataaaag
     1141 aagtaaagca cttacaggtc aggaagtgtg ccagatagaa ttacccctct taaaatagta
     1201 aaaaagccgc attttgcggc ttttttattt gccgggtatt aattttgttt tattgcccat
     1261 gtaaggttgc agtactttag gaatattaac ggaaccatct ttattgagat tgttttccaa
     1321 aaaggcaata agcattcttg gagaagcaat aacggtatta tttaaagtat gggcatagta
     1381 tttgccttct tttccataaa cacggatttt caggcggcga gcctgggcat ctcccagatt
     1441 agaacagctt cctacttcaa agtatttttg ctgtcttgga gaccaggctt ccacgtcaat
     1501 ggacttcact tttaaatcgg ctaaatcccc tgaacagcat tctatggttc ttacgggaat
     1561 atctaaactt ctgaaaatat caacggtgtt ttgccataac ttgttaaacc agtccatact
     1621 gtcttctggt ttacaaataa caatcatttc ttgtttttcg aattggtgaa ttctatatac
     1681 gcctctttct tcgataccat gtgccccttt ttcttttcta aagcagggag aatagctggt
     1741 taatgtatga ggaagggttt cttcgtcaat gatggtatcg atatatttac caatcataga
     1801 gtgttcactg gttcctataa ggtataaatc ttccccttca attttgtaca tcatcgcatc
     1861 catttcttca aagctcataa cgcctgtaac aacattgctt cgaatcataa aaggaggaag
     1921 acaataagta aaaccacgat ctatcataaa atctctggca taggtaagta ctgcagaatg
     1981 cagccttgca atatctccgg ttaaataata aaaaccattt ccggaaaccc ttctggcact
     2041 atcaaggtcg attcctgaaa aactttccat aatatcaata tgatagggga tttcaaaatc
     2101 tggtataacc ggttcgccat atcgttgcaa ttctacgttt tcactgtcat ctttaccgat
     2161 tggaacactg gggtcaataa tatttggaat gaccattagt atgttttgaa ttttttctgt
     2221 taaaactctt tccttttctt cgagttccgc cagacgttcg gcgttggctg tgacttgttt
     2281 ttttaattct tctgcttctt cttttttacc ttgtgccatt aagttaccaa tttctttaga
     2341 gatttttttt ctgtttgctc ttaaattatc ggcttcctgt tggcttgttc ttctttcaag
     2401 atctaaagca attacttcat caaccagagg aagcttatgg tcctgaaatt ttttttggat
     2461 gttttgtttt acgatttcag gattttctct tatgaatttt aaatccaaca tcatcatcac
     2521 ctcttcgaag tattcaattt agattataat acaataaaaa aacctttcaa cccattatat
     2581 atatgggacg aaaggatccg cgttgccacc caaattgctg cttaaaaaac agccactcta
     2641 aagtaggata aaggttacta accattcgac ttttcgccga cagctccaaa agtggaaaga
     2701 tttgttgtta caccggttta caccagccac cggctctctt aagaaacaag caaatcgtag
     2761 cttttatcat cgctgtttat ccatattcgt ctgttacaat gtattatatt atcttttttt
     2821 ttagattgca agccataatt gtgttaaaag taaataaaag ttataccaaa atattattaa
     2881 taaaaacttt tgtttttatt tgcatcctat tttttatttg tatagaataa tgaataagag
     2941 ttttagtatg ggagggaaag ggatgcggat tcaatcttta tcagtatgtg ttcctaataa
     3001 aggatgtgtg aataattgta aattttgtgt atcccatatg agaaatgacg actataaaaa
     3061 tatgatggat gaaaacttac ctttttatga tctttatgaa aaagactata taagaagatt
     3121 agagtttgcc agagataatg gttgtaatac ggttatgtta acaggcaatg cggagcccca
     3181 gcaaaacaga gcttttttac agagattcgg aaccatgaac agtaatctga gtaagccttt
     3241 cagatggata gaaatgcaga cgaccggggt catgatagac gagccttatt taagattttt
     3301 gcgtaatcat gtcggtgtaa gtacccttag cctttcggtt tcttcttttg ataatgaaca
     3361 aaatagagat tataatggca caagtcttaa gaataaagta gatttgattg aattttgtaa
     3421 tagtgtaaag aaatatgatt ttaatttaag aatttctatt aatctaacgg atgcatttaa
     3481 tgactggtct gcggctcgga tatttaaata ttgtaaagag gtatttaatg cagatcaaat
     3541 cactttcagg gttttatacc gttccggttc tgatacagct caggacagat ggattgccga
     3601 gcataaggca aaagatgaat taattgatga aataagaaat tacatattaa ataagggaag
     3661 aaagttagaa aaactggaat ttggcgctat taaatggtcg gtggacgaga tgtcaacggt
     3721 attggatgag gattgtatga gtgaagaagc gaaagaatcg gtaaaatatc ttatcctaag
     3781 acctaattgt aaactttatt ctaaatggga tgataaaggt agtttggttt tctaattttt
     3841 aatggtaacc ttatttgtgg acatattttt
//
LOCUS       DAOSUD010000131         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10390_length_3676_cov_23.305164, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000131 DAOSUD010000000
VERSION     DAOSUD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10390_length_3676_cov_23.305164"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..830)
                     /locus_tag="PLG49_10575"
     CDS             complement(357..830)
                     /locus_tag="PLG49_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17790.1"
                     /translation="MKEDNKTIIDYIGQIFANFGVMILILMALSFIVGDQAKPYSRLF
                     AMADQGLSFETLSQLLLLAVIISLGEILFLTVKWIKNLSVAVRIICFLLMIFITVVIF
                     SIVFSWFSVKDIRAWIGFLLSFSICTAISTAIAALNEKMENKKMEEALKRFHKKR"
     gene            complement(827..1273)
                     /locus_tag="PLG49_10580"
     CDS             complement(827..1273)
                     /locus_tag="PLG49_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ17791.1"
                     /translation="MKMRIEIDINEKYEDLSVEIKSPRLTPDIEKMISMLRILGMQIL
                     GKKDNETFVLDVAEILYIEAVERDTFLYTENEVYESDFKLYELEQQLTERDFFRVSKS
                     TLINLKKVKSLKADLNRRIRVTMVNGEQIIASRMYADELRKRLGIK"
     gene            complement(1270..1962)
                     /locus_tag="PLG49_10585"
     CDS             complement(1270..1962)
                     /locus_tag="PLG49_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17792.1"
                     /translation="MRNICIIFKKQLKDTLKNKTILIQFILFPLMTVVMENSIQLEGM
                     AEHFFAKLFSIMYIGMAPLAAVSSIISEEKEKNTLRVLIMANVKPWQYLMGIGLYVWS
                     ICMLGAGVIGLATAFNHEQFIFFMSVMGLGFMISILAGAAVGILAHNQMMATSVVMPM
                     MMVLSFLPMISMFNEKVEKVAKITYTGQLREILYNMSWDLILNNSIVVIGINAICFLA
                     LFTIAYRKNGLE"
     gene            complement(1974..2822)
                     /locus_tag="PLG49_10590"
     CDS             complement(1974..2822)
                     /locus_tag="PLG49_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ17793.1"
                     /translation="MIEVNNVHKKFNQKVVLKGIHLKISKGEIFGLLGPSGAGKTTLI
                     KILTGQLEFEEGSVKILNKSVKELTGEDRKKLGIMMEQFGLYDRFSCMDNLKIFAEIY
                     RIPYNKVKESLSMVGLEAAAKIPACKLSKGMRSRLQLARVFMHTPQIIFLDEPTSGLD
                     PATSRAIHQMIIAKKEEGCTIFLTTHDMEEATKLCDHVALLNEGKIVEYGSPQEICTK
                     YNHQKRIRLRLYDGRNLDFTHAKDSASEIYSLLLKEQVETIHSSEPNLETVFLELTGR
                     CLQDNE"
     gene            complement(2992..3582)
                     /locus_tag="PLG49_10595"
     CDS             complement(2992..3582)
                     /locus_tag="PLG49_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17794.1"
                     /translation="MSLIVEVPINPKGKTARFYDDRVEFNEKVILYKDIEGLTAGTLG
                     TTVHTYIGIPVGRSFDGGVRFRTKDGKNHNIVMNSFAMFGIPIIRNPKKYEKLFYPLY
                     EAVYSIVAKNMAKPYIDKIRAGATVEVSGFLINSAEAKPVKSRKPIVITKANYRDYQV
                     RDLGDVIVFDRSGDVLWQFTKPNALLIPYIFDEIFA"
BASE COUNT         1196 a          754 c          486 g         1240 t
ORIGIN      
        1 cacaattatt ttgtgttaat caatcaaaat gcacgtttat tattcattct aatcttttta
       61 acaaagttac accttaaagt tttataactt tttattattt ttcttttgca taaaaaaata
      121 cttccccaca gacagccttt tttgacggtc tgtctgaaag tatcctgtca ggtacttgtt
      181 aaaatattta atctgctgtt tatttataat ggaaatggaa gattaaatat acaaaaacgg
      241 ataaaatcta ctatcccata agcaatccta gcagaagtaa tatcaccttt cttttataat
      301 aaagtaaaga tacaaacaac tctttgataa gttcattcca gcgtttaaaa aatatttcat
      361 cgttttttat ggaaacgttt cagggcttcc tccatttttt tattttccat cttttcattt
      421 aatgctgcga ttgccgtact aattgcagta catattgaaa aggaaagcaa aaatccaatc
      481 catgctctga tatctttaac agaaaaccat gaaaaaacga ttgaaaatat tacaactgtt
      541 ataaatatca ttaacaaaaa acaaataatc cttacggcta cagaaagatt ttttatccac
      601 tttactgtaa gaaatagtat ttctcctaaa gaaataatga ccgccaataa aagcaactgt
      661 gataaagttt caaaagacag accctggtct gccatagcaa ataatctgga atatggtttt
      721 gcctggtcac caacgataaa actaagtgcc ataagaatta atatcattac tccaaaattg
      781 gcaaaaatct gtccaatata atctataatg gtcttattat cttccttcat tttattccta
      841 acctctttct taattcatct gcatacatac gggatgcaat aatctgttcc ccgtttacca
      901 tcgtcacccg gattctccgg ttcaagtccg cctttaagct ctttactttc tttaaattaa
      961 tcagagtaga cttacttact ctgaaaaagt cccgttccgt taactgctgc tctaattcat
     1021 atagtttaaa atctgattca tacacttcgt tttcagtata taaaaaggta tctctttcta
     1081 cagcttcgat atacaaaatt tcagctacat ccagcacaaa agtctcatta tctttcttcc
     1141 ctaaaatttg catacctaaa atacgcagca tggatatcat tttttcgata tccggcgtta
     1201 acctgggact tttgatttct actgataaat cttcatattt ttcattaata tcaatttcta
     1261 tcctcatctt cattccaatc cattctttct ataagcaatg gtaaataaag ccaaaaaaca
     1321 aatggcattg attcctatga cgactatgct attatttaaa atcaaatccc atgacatatt
     1381 gtaaaggatt tctcttaact gcccggtata agttatcttc gctacctttt ctactttttc
     1441 attaaacata gaaatcatgg gcaaaaaaga taaaaccatc atcattggca taacaacaga
     1501 agttgccatc atttgattat gggccaatat gcctaccgct gcccctgcta aaatcgaaat
     1561 cataaatcca agacccatca cagacataaa aaagataaac tgctcatgat taaaagctgt
     1621 ggccaatcct ataacgcctg ctcccagcat acaaatactc catacataca gcccgattcc
     1681 catcaaatac tgccagggct tcacattggc cataatcaaa acccgcaggg tatttttttc
     1741 tttttcttcc gatatgatag aagatacagc tgctaaagga gccataccaa tatacataat
     1801 ggaaaaaagc tttgcaaaaa aatgttctgc catgccttca agttgaattg aattttccat
     1861 caccacagtc atcaggggaa ataaaataaa ttgaattaaa atcgtcttgt tttttaaagt
     1921 atccttaagt tgttttttaa aaataataca gatatttctc atgctaatcc ctcctattca
     1981 ttatcctgta agcagcgccc tgttaattct aaaaacactg tttctaaatt gggttccgaa
     2041 gaatgaatgg tttcaacctg ctctttaagg agtaatgaat agatttcact ggcagaatcc
     2101 ttagcatgag tgaaatccag atttcttcca tcatataaac gaagccttat ccttttttga
     2161 tgattatatt ttgtgcaaat ctcctgtggt gatccatact cgacaatttt tccttcatta
     2221 agaagggcga catgatcaca aagctttgtg gcttcttcca tatcatgtgt cgtcaaaaaa
     2281 atggtacagc cttcttcttt tttagcaata atcatctgat gaattgccct gcttgttgct
     2341 ggatctaatc cactggttgg ttcatctaaa aatataattt gaggggtatg cataaaaacc
     2401 cttgccagct gtaatctgct gcgcattcct tttgacagct tacaggctgg gatcttagct
     2461 gcagcctcca gcccaaccat ggataaactc tcttttactt tattataagg aattctataa
     2521 atctctgcaa atatctttaa attatccata catgaaaacc gatcataaag accaaattgc
     2581 tccatcataa tgccaagttt ttttctgtct tctcctgtca attcttttac tgacttattt
     2641 aaaattttta cactgccctc ttcaaattca agttgacctg tcagtatttt tattaatgtg
     2701 gtctttcctg ccccagaagg accaagaagg ccaaaaattt caccttttga tatcttaaga
     2761 tggattcctt ttaaaacaac tttttggtta aattttttat gaacattgtt cacttcaatc
     2821 atttgccaat gcctcctttc tttagcccca gcgtatcata aaaaaagcct gtctttctca
     2881 cattttgcat aagttgcact tttgacaaca caagatgcca aaaataaaag ccgtcaaaag
     2941 cattcagctt gacggctctt atcaatctta ttatattttt tcaagttttt attaagcaaa
     3001 aatttcatca aagatatatg gaattaatag tgcattgggc ttggtaaatt gccacaaaac
     3061 atcacctgac ctatcaaaga caatgacatc tccaagatct ctaacctgat aatcccggta
     3121 atttgctttg gtaattacaa taggctttct tgactttacc ggctttgcct ctgcactgtt
     3181 aattaaaaat ccggaaactt ccactgtagc accagccctt attttatcaa tatatggctt
     3241 tgccatattt tttgccacaa tagaataaac ggcctcatac aatgggtaaa agagtttttc
     3301 atatttttta ggatttctta taatgggtat tccaaacatt gcaaaggaat tcataacgat
     3361 attatgattt ttgccatctt tcgttctaaa acgaacacct ccatcaaaac tgcgtcctac
     3421 aggaatacca atatatgtat gaactgttgt tcctaaagta cctgctgtca aaccttcaat
     3481 atccttatac agaatgactt tttcgttaaa ctctacacgg tcatcataaa aacgagcagt
     3541 ttttcctttg ggattaatgg gcacttctac aatcaacgac atatagattt cctctccctt
     3601 atagtaagat atataaataa atcatcaaaa caagccaaaa agttaaagtg aaatataaat
     3661 tcttgtcatc aaacag
//
LOCUS       DAOSUD010000132         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10625_length_3600_cov_27.293935, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000132 DAOSUD010000000
VERSION     DAOSUD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10625_length_3600_cov_27.293935"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..943)
                     /gene="galU"
                     /locus_tag="PLG49_10600"
     CDS             complement(56..943)
                     /gene="galU"
                     /locus_tag="PLG49_10600"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOQ17795.1"
                     /translation="MKIRKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQFIVEEA
                     VESGIEDIIIVTGRSKRSIEDHFDKSIELELELQKKGKEDLLELTRSVSDLANIHYIR
                     QKEPKGLGHAILTARNFIGNEPFAVLLGDDIIVSEKPCLKQMIEVFNEYNTSILGVQQ
                     VPYEDVIKYGIIKGKNIRDRIYEVSDMVEKPKREEAPSNVAILGRYIITPTIFDFLEK
                     QETGAEGEIQLTDALKNLCKVEDIFAYDFIGKRYDVGNKMGFLQATVEFALRRDDLRE
                     EFLEYLNYILNNLNDINPK"
     gene            complement(974..1492)
                     /locus_tag="PLG49_10605"
     CDS             complement(974..1492)
                     /locus_tag="PLG49_10605"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOQ17796.1"
                     /translation="MENNQTGQKVILSITGIQKYDDFDNSIEVVAPGKYYVKDNKQYL
                     AYKERNEGASQDILTTVKIEHDKISVMRFGGINTNMTFELNKKHTTFYDTEHGALTVS
                     VTAKKIRVELDEEEAKGEIEVVYEFEIDHMLIGINILYIKMHPTSENENNASCEDLSL
                     DDAFLNDYLQEK"
     gene            complement(1574..2380)
                     /gene="murI"
                     /locus_tag="PLG49_10610"
     CDS             complement(1574..2380)
                     /gene="murI"
                     /locus_tag="PLG49_10610"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOQ17797.1"
                     /translation="MDSRPIGVFDSGVGGLTVVQEIMNQLPNEEIIYFGDTARVPYGS
                     KSKETVTKYSKQIIRFLLTKNIKAIIIACNTASSNSLEEVRKTFDIPIIGVVKPGAVM
                     AYKTTKNKRIGIIGTEATIRSESYSKEIKKHDKEIETFSVPCPLFVPLAEEGWTDNEI
                     SYLVAKKYLEPFADLEIDTLVLGCTHYPLLKDVIQNVVGNKVQLINPALETAKDLKEL
                     LSAGNALREDKTAPNHSFYVSDNTSKFEQIAAIALKKSIPPVEIIDIEQY"
     gene            complement(2395..3465)
                     /locus_tag="PLG49_10615"
     CDS             complement(2395..3465)
                     /locus_tag="PLG49_10615"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOQ17798.1"
                     /translation="MNSKKTVVVLFGGQSSEHDVSRISASTIIKNMDPNKYFVLTVGI
                     TKEGKWMLYNGPVNHIETGEWEKFATPAFISPDATQKALIKFAGDRYKTIPIDVVFPV
                     LHGLYGEDGTVQGLLEMAQIPYVGCGVLASSVSMDKVYTKMIVEKENIAQAKYLVIYK
                     EDLNDIEPIVSKVNEKIGYPCFIKPSNAGSSIGITKAHNQEELINGLHIAAKHDRKIL
                     VEEAIVGREVECAVLGNLEVKASSVGEIIAAAEFYDYDAKYNNSESKTIINPDIPTEI
                     AEEIREKSVRIFKAVDGSGLSRVDFFIEKDTNRVIFNEINTMPGFTSISMYPMLWENA
                     GIPIGKLIDTLIELAIERVQNK"
BASE COUNT         1073 a          727 c          481 g         1319 t
ORIGIN      
        1 tggattacca tgagttttct atatattacg gcattttatt ttacaacttc cccttttatt
       61 tggggtttat atcatttaaa ttattcaaaa tataattaag atactctaaa aattcttccc
      121 ttaaatcatc tcttcttagg gcaaattcaa cggttgcttg taaaaatccc attttattac
      181 caacatcata tcgttttcca ataaaatcat aagcaaaaat atcttctact ttacataaat
      241 ttttcagggc atctgttaac tgaatttctc cttcagctcc cgtttcttgc ttttcaagaa
      301 aatcaaaaat cgtaggtgta ataatatatc tgccaagaat agcaacattg gaaggtgctt
      361 cttccctttt cggcttttct accatgtcac ttacttcata aattctgtcc cgaatatttt
      421 taccctttat aataccatat tttattacat cttcataagg aacttgctgt acacctaaaa
      481 tagaagtatt atattcatta aatacttcta tcatctgctt taaacaaggt ttttcagaaa
      541 caataatgtc atctcctaat aaaacagcaa aaggttcatt ccctataaaa tttcttgcgg
      601 ttaaaatagc atgtcctaag cctttgggct ctttttggcg gatatagtga atattcgcta
      661 aatctgaaac gcttctggtt aattccagta gatcttcttt accttttttt tgcaattcta
      721 attccagttc tatggattta tcaaaatggt cttcaatcga tcttttactt ctacctgtta
      781 cgataataat atcttcaatt ccggattcta cagcctcttc tacaataaat tgaattgttg
      841 gcttatctac aatgggaagc atttctttgg gttgtgcttt ggtcgcaggc aaaaatcttg
      901 ttccgagacc tgctgcaggt ataattgctt ttctaatttt cattgtctca gctcctatat
      961 gatgtattta tatttacttt tcctgaagat aatcatttaa aaaagcatca tccaaactga
     1021 ggtcctcaca ggaagcattg ttttcatttt cactggttgg atgcattttt atgtaaagaa
     1081 tattgattcc tataagcatg tgatctattt caaattcata aacaacctct atttctcctt
     1141 tggcttcttc ttcatctaac tccacacgga ttttctttgc ggttaccgaa accgttaaag
     1201 ccccgtgttc tgtatcataa aaagtagtat gctttttatt caattcaaaa gtcatattgg
     1261 tgttaattcc accaaatctc attacggata tcttgtcatg ctctatttta accgtcgtta
     1321 aaatatcttg agaagcacct tcatttcttt ctttataggc aagatattgt ttattgtctt
     1381 ttacataata tttccccggc gcaacaactt caattgaatt gtcaaagtca tcatactttt
     1441 gaattcccgt aatcgataag atgactttct gtcctgtctg gttattctcc atatattcac
     1501 tccttgttaa atcctagtgt cattataccg ataaaaaagg caatttacaa ggaaagaaag
     1561 ctaagattat tttttaatat tgctctatat ctataatttc taccggagga atagattttt
     1621 ttagagcaat ggctgctatt tgttcaaatt tactggtatt gtcactcaca taaaaagaat
     1681 gattaggagc agttttatct tctcgtaaag cattgcctgc acttaataat tcttttaaat
     1741 cttttgccgt ttcaagagct ggattaatta actgtacctt attgcctacc acattttgta
     1801 taacatcctt taacaaggga taatgggtac agccaagaac taaagtgtca atttctaaat
     1861 ccgcaaaagg ctctaaatat ttttttgcca ctaaataact aatttcatta tccgtccagc
     1921 cctcttcagc caaaggcaca aaaagagggc atggaacaga aaaggtctct atttctttat
     1981 catgcttttt tatttcttta ctataacttt ctgaacggat ggtcgcttcc gttccaataa
     2041 ttccaatccg cttatttttt gttgttttat aagccattac tgcccctggc tttacaacac
     2101 ctataatagg aatatcaaaa gttttcctga cttcctctaa actattggaa cttgctgtat
     2161 tgcaagcaat aataattgcc ttaatatttt tggtcagtaa aaatcgaatg atctgctttg
     2221 aatattttgt aacagtttct ttggacttgc ttccataagg aaccctggcg gtatctccaa
     2281 aataaatgat ttcttcattg ggcagttgat tcataatttc ctgaaccact gtaagtcctc
     2341 cgaccccgga atcaaatacc cctatcggac gggaatccat ctatacacct cttctcattt
     2401 gttttgcact ctttctatag ctaattcaat taaagtatca attagcttgc ctattggtat
     2461 gccagcattt tcccaaagca ttggatacat gctgattgaa gtaaagcctg gcatcgtatt
     2521 aatttcatta aaaataaccc tgttcgtatc tttttcaata aaaaagtcca ctcttgaaag
     2581 accagaaccg tcaactgctt taaaaattct aaccgatttt tctcttattt cttctgcgat
     2641 ttctgtagga atatccggat taatgatggt ttttgattca ctattattat attttgcatc
     2701 ataatcataa aattcagcag ctgcaataat ttctcccaca gacgaagcct taacttccag
     2761 attgcctaaa accgcacatt cgacttcccg gcctacaata gcttcttcta ctaaaatctt
     2821 tctgtcatgc tttgccgcta tatgaagacc attaatcagt tcttcctgat tatgcgcctt
     2881 tgtaattcct atggaagaac ctgcattaga tggcttaata aaacaaggat aacctatctt
     2941 ttcattaacc tttgatacaa taggctctat gtcattcaaa tcttctttat aaattacaag
     3001 atatttagcc tgagcgatat tttctttttc aacaatcatc ttggtatata ccttatccat
     3061 agaaacactg gaggccagga caccacagcc cacataagga atttgggcca tttctaaaag
     3121 tccctgaacc gttccatctt caccatacaa accatgaagt acaggaaaaa ccacatctat
     3181 aggtatcgtt ttatacctgt ctcccgcaaa tttaattaaa gctttttgtg tggcatcagg
     3241 actaataaaa gcaggtgtag caaacttttc ccattctcct gtttcaatat gattaaccgg
     3301 accattatat aacatccatt ttccttcttt agtaatcccc acagttaaaa caaaatattt
     3361 attaggatcc atattcttta taattgtgga agcagatatc cgtgaaacat catgttctga
     3421 agattgtccc ccaaataaca caacaactgt ttttttacta ttcatgatta aatccctctt
     3481 tccaaattat cgatattact attctatgat ttaggctcat catattcaac aaatatttgt
     3541 ttttatttcc ataccatttt aatgttttat atgtatgaat tcaagtcatt ttataaatac
//
LOCUS       DAOSUD010000133         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10650_length_3591_cov_26.860860, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000133 DAOSUD010000000
VERSION     DAOSUD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10650_length_3591_cov_26.860860"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLG49_10620"
     CDS             complement(<1..395)
                     /locus_tag="PLG49_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ17799.1"
                     /translation="MSELIIMSDSSCDIPDSVIKEYNIKLIPYYVSFDQKNYYKERIE
                     LSIKDFYEKLRKENVFPKTSLPSVDDYMNAFTPLIQEGKGIICFCLTSKFSGSYQAAV
                     NAKNILLEEYPDAKIEVINSIQATGGQGLV"
     gene            565..1002
                     /locus_tag="PLG49_10625"
     CDS             565..1002
                     /locus_tag="PLG49_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOQ17800.1"
                     /translation="MLTPSLQDYLEEIYRLSIKSDEIRVSDIAEVLEVSLPSVVKALH
                     KLNSRGYILYQPYEPVKLTEKGIREGKYLVQRNQLLKDFLRVLKTNSDIEKEAESMEH
                     YLSLSTIQAIEKFYHFMKDNPDIQDRYDVYLQKTNENYIFEND"
     gene            complement(1068..2420)
                     /locus_tag="PLG49_10630"
     CDS             complement(1068..2420)
                     /locus_tag="PLG49_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOQ17801.1"
                     /translation="MSDYSHTLSADQFRDHIVTSLYKRAKEIEEAVVTYSKENKSTLD
                     DKIDSILTSRLFGFPIMLLILALIFWITIVGANFPSKILADLLFSLEDKLTELFINFN
                     APSRLHGIVVLGMYRTAAWVISVMLPPMAIFFPCFTLLEDLGYLPRVAFNLDRIFKKA
                     GAHGKQALTMAMGFGCNAAGVISSRIIDSPRERLIAILTNNFVPCNGRFPTLIAISMI
                     LIGASSDSLLNSLFASGLVVLLILLGIAMTFLISFIASKSFLKGTPSRFSLELPPYRK
                     PQIGKIIIRSIFDRTLFVLGRAVAVALPAGAITWLLANISIGNTTILNHIANFLDPFA
                     LWIGLDGVILLAFFLGLPANEIVLPIIFMIYLSTGSMIEIDSIEEMRQLFLAKGWTHL
                     TCLNVMLFSLLHFPCGTTLWTIKKETQSVKWALASFIIPTLTAILICFISTQIASLFF
                     "
     gene            complement(2407..3180)
                     /locus_tag="PLG49_10635"
     CDS             complement(2407..3180)
                     /locus_tag="PLG49_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOQ17802.1"
                     /translation="MGLTCNACKRKAMQDTYHIKKSNPNQMIIALAGNPNTGKSTIFN
                     ELTGLKQHTGNWPGKTVTNAWGIFKHKNQEFILVDLPGTYSLFPNSAEEEIARDFICF
                     SKPDATVIVVDASCLERNLNLVLQIIEITHTVILCVNLLDEARRKKIKVDLEKLSVEL
                     GIPVVGTIARDKKGLEDLKDIIYKVCSKKIIPHPKMTTYPKEIEDEIQKLIPMIEPLV
                     KNKINPRWVALQMLDGDLRILEKITNYPNCKGGCHDVRL"
     gene            complement(3191..3436)
                     /locus_tag="PLG49_10640"
     CDS             complement(3191..3436)
                     /locus_tag="PLG49_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ17803.1"
                     /translation="MYEKYIPLHQVHIGKTCIIKELKATGSQRRRLLDLGFTQDTPIT
                     VIRRSPLGDPTAYLIKKSIIALRQEEASKILVEIIRN"
BASE COUNT         1188 a          588 c          674 g         1141 t
ORIGIN      
        1 acaagaccct gaccaccagt tgcctgaata gagttaatga cttctatttt ggcatctgga
       61 tattcttcaa gaagaatatt tttagcatta acagcggctt gataagaacc gctaaattta
      121 ctggtaagac aaaaacaaat aatacctttg ccttcttgaa taagaggagt aaaggcattc
      181 atataatcgt ctacactggg cagggatgtt ttaggaaaaa cattttcttt tcttaatttt
      241 tcataaaagt ctttaatact taattctatt ctctctttat aatagttttt ttgatcaaag
      301 gacacatagt atggaataag tttgatattg tattctttta tgacagaatc gggtatatca
      361 caggaactat cggacataat aataagttca gacatgaata gtcctccatt cgcttgcaga
      421 tattcagtac tattgtaata tgcgtattta ttaatgtcaa ttcagttttt actggataag
      481 ttttaagctg ggatattgaa ggaatatcct gtatattttt tgaatatatt ggttgtgaat
      541 aaaaaatgat tggagtttca aaatatgtta acaccaagtc ttcaggatta tttagaggaa
      601 atttatagat tgtcgattaa atccgatgaa ataagggtat ctgatattgc agaagtactg
      661 gaggtgtcct taccgtctgt tgtaaaagcc ctccataaat tgaacagcag aggttatatc
      721 ttgtatcagc cttatgagcc ggtaaaacta acggaaaaag gaattcggga aggaaaatat
      781 ctggttcaaa gaaatcaatt attaaaagat tttttaaggg ttctaaaaac caattcagat
      841 attgaaaaag aagccgaatc aatggaacat tatttatctt tatcaacgat acaagccata
      901 gaaaagtttt atcactttat gaaggacaat ccggatattc aagacaggta tgatgtatat
      961 ctccaaaaaa caaatgaaaa ttatattttt gaaaatgatt agttctataa aaaacaaaaa
     1021 tataaggaaa aatttaattt taccttatat tttttgcaaa ctattgatta gaaaaataag
     1081 gaagcaatct gggtgcttat gaaacatatc aatatggctg taagggttgg aatgatgaag
     1141 gaagccaggg cccatttgac actttgtgtt tcttttttta tggtccacag agttgttccg
     1201 catggaaagt gaagaagaga aaataacatc acatttagac aggttagatg agtccagccc
     1261 ttagctagaa acagttgtct catttcttca atactgtcga tttcaatcat agaacccgta
     1321 gataagtaaa tcataaaaat aatgggtaat acaatttcgt tagctggcaa accaagaaag
     1381 aaagctaaga gaatgacacc atccaggcct atccatagcg caaaaggatc caggaagttg
     1441 gcaatatggt tcaatatggt tgtatttcca atgctaatat tggcaaggag ccaggtgatg
     1501 gcacctgcag gaagagccac agccacagcc cgcccaagaa caaataaagt acggtcgaag
     1561 atggagcgaa tgatgatttt tccaatttga ggttttctgt aagggggcag ttccaggctg
     1621 aatctggaag gtgttccttt caggaaactt ttagatgcaa taaaggatat aaggaatgtc
     1681 atggcaattc ccaataaaat cagtaaaacg accagaccgg aagcaaacag ggaatttaat
     1741 aaagaatcag aggatgctcc aattaatatc atggagatag caattaaagt agggaatctt
     1801 ccattacagg ggacaaaatt attggtaaga atagcaatga gtcgctctct tggtgaatca
     1861 ataatacggc tggatataac gcctgctgca ttacagccaa agcccatagc catggttaaa
     1921 gcttgtttgc catgggctcc ggctttttta aatattcggt ctaaattgaa agcaaccctg
     1981 ggaagataac ccaagtcttc taagagagta aagcaaggga aaaaaatagc cataggaggt
     2041 agcataacgg agatgaccca tgctgcagta cggtacattc ctaagactac aattccatgc
     2101 agcctggagg gcgcattaaa attaataaat agttctgtta atttatcttc taaggaaaac
     2161 aaaagatctg ccaggatttt ggaggggaaa tttgctccaa caattgttat ccaaaaaatc
     2221 aatgccaata ttagaagcat aataggaaag ccaaataatc ttgaagttag aatagaatcg
     2281 attttatcat caagcgtcga tttattttct ttagaatagg tcactacggc ttcttcaatt
     2341 tctttagcac gcttataaag actggtgaca atatggtctc tgaattgatc agccgacagg
     2401 gtatggctat agtctgacat catggcatcc tcctttgcaa ttaggataat tggttatttt
     2461 ttcaagaatt ctcaggtctc catccagcat ttgaagagct acccagcggg gatttatttt
     2521 gtttttaaca agaggctcaa tcataggaat cagtttttgg atttcatctt caatttcttt
     2581 tggataagtt gtcatttttg gatgaggaat aattttttta gaacagactt tgtaaatgat
     2641 atcttttaaa tcttctaatc ctttcttgtc tcttgctatt gttcctacta cagggatacc
     2701 taattccaca gaaagttttt ctaaatcaac ttttattttt ttccttcttg cttcgtctaa
     2761 aagattgacg cataaaataa cggtatgcgt tatttctatg atttgtaaaa ccaaattaag
     2821 atttctttcc agacaactgg catctaccac aattacggta gcatcgggct tactaaaaca
     2881 aataaaatcc cttgcaattt cttcttcagc agagtttggg aagagggaat aagttcccgg
     2941 taaatcaact aaaataaatt cttgattttt atgcttaaaa ataccccatg cattagtaac
     3001 agtttttcca ggccaattgc ctgtatgttg ttttagtccg gtcaattcgt taaaaatagt
     3061 acttttacct gtgtttggat tacccgctaa ggcaataatc atttgatttg gattgctttt
     3121 tttgatatga tatgtgtctt gcatggcttt tcttttacaa gcgttacagg ttaatcccat
     3181 aaaaatcccc ctaattcctt atgatttcta ctaaaatttt tgaagcttct tcctgacgta
     3241 aggcgataat actttttttt attaaataag cagtagggtc tcccaggggg ctgcggcgga
     3301 taaccgtaat gggggtatcc tgggtaaagc ctaaatcaag aagtctcctt ctttggctgc
     3361 ctgttgcttt taattcttta atgatgcagg tcttgcctat atgaacttga tgaagaggaa
     3421 tatatttttc gtacaaaaca aaccgcccct ttttataatg ttagtcttgg caaatatgct
     3481 atcttagcac agtatcattt tattctgcaa agaagaaaag gaaacttgtc tctagaaaat
     3541 ggcagaaaaa gatcactgag cttttatcga cagcaaaatt tgattaattt t
//
LOCUS       DAOSUD010000134         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10776_length_3553_cov_24.848485, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000134 DAOSUD010000000
VERSION     DAOSUD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10776_length_3553_cov_24.848485"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..594)
                     /gene="rnpB"
                     /locus_tag="PLG49_10645"
     ncRNA           complement(237..594)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG49_10645"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(678..2351)
                     /locus_tag="PLG49_10650"
     CDS             complement(678..2351)
                     /locus_tag="PLG49_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ17804.1"
                     /translation="MIETATNDFVKVTFSNGEVEKYPKGIKLEEIVKEKQPFYKYTIV
                     AAKVGNNMEELRHEITEDCHLEFIDLTTTDGMRIYQRSLSFLLFYVVRTLIKDGNVII
                     HHSVGPGFYCELIGVDHLNQELLNKIENKMRELVEQDLPFNKMSIPLEEAMKIFEENG
                     MDDKKNLFKYRRSSMVNLYELGGVKNYFYGYMVPSTGFLKHFRLILDGSGFILQFPTT
                     KDPTKVPEFVPSPKLSQIFLEYKNWGKILRVDTVGALNDVIANQDIRSLIMTAEALHE
                     KKIAQIADMIAEKKDSIAVVLIAGPSSSGKTTFAQRLAIQLRVNGLRPHAISIDDYFV
                     NREFTPRDENGEYDFESLESIDLELFNHDLKRLINGEVVEIPSFNFKTGKREYKGRKI
                     KMESNDILIIEGIHGLNERLTKDIHKDNKFKIYISALTQLNIDDHNRIPTTDTRLLRR
                     IVRDNQYRGTSAERTIELWPSVRRGEEKNIFPYQEEADVMFNSALIYELSVLKQYAEP
                     LLFRIEPQSPCYSEAKRLFKFLDYFLGVSSEAVPQNSIIREFIGGSCFH"
     gene            complement(2415..3221)
                     /locus_tag="PLG49_10655"
     CDS             complement(2415..3221)
                     /locus_tag="PLG49_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOQ17805.1"
                     /translation="MAVKCKDIIRKMNEFADERLAEDWDNVGLLVGDLEQEIKTVLVA
                     LDAVPQVICEAIELKADLIITHHPLIFRAIKSVTKGNPLGDSIYQLIQNNIGLYCAHT
                     NLDIAYGGTNDSLAQILELENISLLEEISEEEGKKYGMGRIGTLKKPLKLKDYALFVK
                     EQLNLSDIRIAGDMDKIINKVAITTGSGMSYLKSAIMKKADLFITGDIKFHDAQEAQK
                     YGICLLDAGHYGTEVIIVKVIKDYLERIQEENFQFKVLLSKVNGEPFITL"
     gene            complement(3212..>3553)
                     /locus_tag="PLG49_10660"
     CDS             complement(3212..>3553)
                     /locus_tag="PLG49_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016429 - tRNA
                     (adenine-N1-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HOQ17806.1"
                     /translation="IQPQLDQNEVRKYLHSINHKIINEQLVYEDNKYYWIMSSEAGKE
                     KYDKEIFYLYSKILIEKKDPILKQYIEKEIKKNKNILERLNHPLTEHTLRRKKEIEKN
                     IRDCEEVLLWL"
BASE COUNT         1036 a          723 c          504 g         1290 t
ORIGIN      
        1 aaatcattaa tttactttat tattacaact gaatagatct gcttaaattc taaattcatt
       61 tttatattaa acatgcatca tttggtaatt ttacaaatta taagcctggt cgttataaat
      121 tttgccaaaa aaataagcat cctttattgg atgcccacct tatcacgtca aaaaattact
      181 aatcctttaa aataaaaaac atctacaaag tagatgtttc aaatgattca ataaagtcta
      241 tgagcaaatc tgtaagccga gttctgttgt atacggtcat ctatctaggc atatagttac
      301 ctatatgctc cagcaaccta cccgagaacg tgacgagcag ccacaaatgt tctcctatac
      361 ggtcttgctc caggtggggt ttacagagcc tcccaagtcg ccatgggagc ggtgagctct
      421 tacctcacct ttccaccctt actgccttat tataataaga cagcggttta tttctgttgc
      481 actttcctta gagtcgcctc cactgggtgt tacccagcac cctgctctgc ggagctcgga
      541 ctttcctcaa gtacgacctt tcggtactgt acttgcgacc gtacgatttg ctcattaata
      601 tatttttttc taataacata ataacatgct tataaaagta aatcaatgta atttatttac
      661 atttttatta tatatttcta gtgaaagcaa ctacctccaa taaattctct gataatagaa
      721 ttttgtggaa cagcttcact gcttacccct aagaaataat ccaggaattt aaatagcctt
      781 tttgcttcag aatagcaagg agattgaggc tcaattctaa aaagcaaagg ttcagcatat
      841 tgctttaata cggataattc ataaatcagt gcagagttaa acataacatc tgcttcttcc
      901 tggtatggga aaatattttt ttcttctcct cttcttacag aaggccacaa ttctatggtt
      961 ctttctgcac ttgttcccct gtactgatta tcccttacaa ttcttctcag cagtctggta
     1021 tccgttgtag gtatcctgtt gtgatcatca atatttaact gggtcagagc actaatgtaa
     1081 attttaaatt tattatcttt atgtatatcc tttgttaacc tttcgttcaa tccgtgaata
     1141 ccttcgatga tgaggatatc gttactttcc atttttatct ttctgccctt gtattctctt
     1201 tttcccgtct taaaattaaa ggaaggtatt tcaaccactt caccattaat taatcttttt
     1261 aaatcatgat taaacagttc tagatcaatg gattccaaag attcaaaatc atattcccca
     1321 ttttcatccc ggggggtaaa ttccctgtta acaaaataat cgtctatgga aattgcgtga
     1381 gggcgcaagc cgtttactct aagctgaatg gccagtcttt gagcgaaggt cgtcttaccg
     1441 gaagaagaag gcccggcaat taagaccacg gctatagaat cttttttttc tgcaatcata
     1501 tcagcaatct gggcaatttt cttttcatgg agtgcttcag ccgtcataat taatgaccta
     1561 atatcctgat ttgctattac atcgtttaaa gcccctaccg tgtcaactct taagatcttt
     1621 ccccagtttt tatattctaa aaaaatttga gacagttttg gtgacggtac aaattctggt
     1681 accttcgttg gatctttagt cgttgggaat tgcaaaataa aacctgaacc gtctaaaatc
     1741 aatcgaaaat gctttaaaaa tcccgtgcta ggcaccatgt agccataaaa ataattcttt
     1801 acgcctccca gttcatatag atttaccatg gaagaacgtc tgtatttaaa caagtttttc
     1861 ttatcatcca ttccattttc ctcaaagatt ttcatggctt cctctaaagg aatggacatt
     1921 ttattaaaag gcaaatcttg ttcgaccagt tctctcattt tgttttctat tttatttaac
     1981 aactcctggt ttaaatgatc tacccctatc agttcacagt aaaaacctgg acccacagaa
     2041 tgatgaataa taacattgcc gtcttttatt aatgttctga caacgtaaaa aagtaagaaa
     2101 gataaactcc tttgataaat tctcattccg tcagtagtcg ttaaatctat aaattccaaa
     2161 tgacagtctt cagtaatttc atgtcttagc tcttccatat tatttccaac cttagcagca
     2221 acaatggtat acttgtaaaa gggctgtttt tcttttacaa tttcttctaa tttgattccc
     2281 ttgggatatt tttctacctc tccattagaa aaagttactt ttacgaaatc atttgttgca
     2341 gtttctatca taacgattct ccttccatcg cagaataatg gcttctacct cttattacta
     2401 cagatcttcc gaccttataa tgttataaaa ggttccccat ttacttttga aagcaatact
     2461 ttaaactgaa aattttcttc ctgaatacgc tcaagataat cttttataac ctttacgatg
     2521 ataacttctg tcccataatg accggcatct aaaagacaga ttccatattt ctgagcttcc
     2581 tgggcatcgt gaaatttaat atctcccgtt ataaataaat ctgctttttt cattatagca
     2641 gatttcagat aagacatgcc acttcctgtt gtaatggcta ctttattaat tatcttatcc
     2701 atatcgcctg caatccgaat atccgaaaga ttaagctgtt ctttaacaaa taaagcatag
     2761 tctttaagct taagaggctt ttttaaagtc cctattctgc ccattccata ctttttgcct
     2821 tcttcctctg atatttcttc caaaagggat atattttcta attccagtat ttgggctaag
     2881 ctatcatttg tacctccata ggcaatatcc agattcgtgt gggcacagta gagaccaata
     2941 ttattttgaa tcagctgata aatgctgtct cccaaagggt ttccctttgt aacagacttt
     3001 attgccctaa aaattaaagg gtgatgggta atgattaaat ctgcttttaa ttctatggct
     3061 tcacaaatga cttggggtac agcatctaaa gccactaaaa cggtcttgat ttcttgttcc
     3121 aaatctccga ctaacaatcc aacattgtcc cagtcttcag ctaatctttc atcagcaaat
     3181 tcattcattt ttcttatgat atctttacat tttacagcca taaaagcacc tcttcacaat
     3241 ccctgatgtt tttttctatt tcttttttcc gccttaacgt atgttctgtt aaaggatgat
     3301 tcagtcgttc caatatattt ttgttctttt ttatttcctt ttcaatgtat tgctttaaaa
     3361 tgggatcttt tttttctata agaattttac tgtacaaata gaagatttct ttatcatatt
     3421 tttctttacc tgcttctgaa ctcataatcc aataatattt attatcttca tatacaagct
     3481 gctcattaat tattttatga ttaatactat gtaaatattt tctgacttca ttttggtcca
     3541 attgaggctg aat
//
LOCUS       DAOSUD010000135         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10807_length_3545_cov_22.717479, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000135 DAOSUD010000000
VERSION     DAOSUD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10807_length_3545_cov_22.717479"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLG49_10665"
     CDS             <1..933
                     /locus_tag="PLG49_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ17807.1"
                     /translation="KKTMFVSMEAKYQKLKRKFYIVMAILVLLIIANLYYIYLNYDYL
                     AFKHLISQNYIYTDSLDKLFERELKRDVNGQYYKYFDNLVISIVTREIREEQPDPYTY
                     QYLPEQYKKSKENEKKKADQSYVDELDENIVYLKLTNYSKHTKNFVKNEKETLEKYTN
                     IIIDLRDNPGGDVFALNYIADLFLPEDNIISYDNTRSKLFSKTVKSKKKKQLSYDKII
                     ILQNNDTASSAEGFIAALKDNLDNVILIGDTSYGKGTGQIIIPLKKNFAVKATTMFWN
                     TPKNQNVQGTGIEPDIRYTEDDIIEYAVKYIKEK"
     gene            complement(954..3095)
                     /gene="rnr"
                     /locus_tag="PLG49_10670"
     CDS             complement(954..3095)
                     /gene="rnr"
                     /locus_tag="PLG49_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOQ17808.1"
                     /translation="MEERKQLILDLMSHKDYRPMKIKELSMLLQVPNHERAALEEILN
                     QLIQEGKIIRTKKDKYSLPETLNMVIGIFQGNAKGYGFLLLEDSDANDIFIPADKVNG
                     AMHKDKVLCKIIKAEHGGRKAEGEIVQILERGSEKVVGTYEKSKNFGFVIPDGKNFSK
                     DIFIPKGDDLGAVTGHKVVVKITDWGKDRRNPEGEIIEILGHINDPGTDILSIIKEFD
                     MPMDFPEEVMKEIESVPDELSEEDIKGREDLRAIRMVTIDGEDAKDLDDAISLEKLPN
                     GLYRLGVHIADVSHYVRENTALDKEAYKRGTSVYLVDRVIPMLPHKLSNGICSLNAGE
                     DRLALSCIMDIDSAGVVKNHRVVESVINVNERMTYTNVKKILVDKDEELISKYKDYVD
                     FFKLMEELCIILREKRIKRGAIDFNFEEAKIILDEKGKAVDVQIVERNIATRIIEEFM
                     LVCNETIAEDFYWRGVPFIYRTHEDPDSEKILTLSSFIHNFGYRIKGASKVHPKDLQK
                     ILFDIEGKPEEAVISRLVLRSLQQARYTYTNDGHFGLAAKNYCHFTSPIRRYPDLQIH
                     RIIKAVIHNKLDEKTTKRLNKIMPEVAKHCSIRERVADDAERQTEQLKKVEYMMDKIG
                     ERFDGIISGLTSWGMYVELPNTVEGLVHVTEMDDDYYIFDEAHYLLIGEKRKKIYRIG
                     DKVKVKVLNADLIQRTIDFSLVLPEDDIEEE"
     gene            complement(3226..3468)
                     /gene="secG"
                     /locus_tag="PLG49_10675"
     CDS             complement(3226..3468)
                     /gene="secG"
                     /locus_tag="PLG49_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOQ17809.1"
                     /translation="MSTLTIVLSVVYVILALILITAVMLQEGKSAGLGTLSGSNSDTF
                     WGKNKAYSLEGQLEKFTKILAVLFIVLAVVLVVLIK"
BASE COUNT         1168 a          696 c          478 g         1203 t
ORIGIN      
        1 aaaaaaacca tgtttgtttc tatggaagcc aaataccaga agctaaaaag gaaattttat
       61 attgtaatgg ctattttagt tctcctgatt attgcaaatc tatactatat ttatttgaat
      121 tatgattact tagctttcaa acatttaatt agtcaaaact atatttacac agacagtctg
      181 gacaaactct ttgagagaga attaaaaaga gatgttaatg gacaatatta caaatacttt
      241 gacaatcttg tcatatctat tgtaacacgg gaaatcagag aagagcaacc tgacccttac
      301 acttatcaat acttacctga acagtacaaa aaaagtaagg aaaatgaaaa aaagaaagcg
      361 gatcaatcct atgttgatga gctagacgaa aatattgttt atctcaaact caccaattac
      421 tctaaacaca ccaaaaattt tgtaaaaaat gaaaaagaga ctttggaaaa atacacaaat
      481 atcattattg acctcaggga taatcccggc ggagatgttt ttgccttaaa ttatatagcc
      541 gatctgttcc tgcctgaaga taatattatt tcttatgata atacccggtc aaaattattt
      601 agtaaaaccg taaaaagtaa aaagaaaaaa caattaagtt acgataaaat cattattctg
      661 caaaataatg atactgccag ctctgctgaa ggctttattg cagctctgaa agacaattta
      721 gataatgtta tattaatagg cgataccagt tatggtaaag gaactggaca aatcatcatc
      781 cctttaaaaa agaattttgc agtaaaggca acaaccatgt tttggaatac gcctaaaaat
      841 caaaatgtcc agggaacagg gattgaacct gatattaggt atacagagga tgatattata
      901 gaatatgctg ttaagtatat aaaagaaaaa tagctcaatg agctattttt cttttattcc
      961 tcttcaatat catcctctgg cagtactaaa gaaaagtcaa ttgttctttg aataagatct
     1021 gcattaagca cttttacttt taccttatct ccaatccgat aaatcttttt tctcttttct
     1081 ccaataagta aataatgcgc ttcatcaaag atataataat catcatccat ttctgtcaca
     1141 tgaacaagac cctcaaccgt attgggcagt tccacataca taccccagga agtcagacca
     1201 gaaataatcc cgtcaaacct ttctccgatt ttatccatca tatactccac ttttttcaat
     1261 tgttctgttt gcctttcagc atcgtcagca actctttccc ttatagaaca gtgttttgca
     1321 acctcaggca ttattttatt tagcctctta gtggtttttt cgtctaattt attatgaatg
     1381 accgctttaa taattcgatg aatttgtaaa tccggatatc ttctaatggg ggaagtaaaa
     1441 tgacagtaat tctttgctgc caaaccaaag tgtccatcat ttgtataagt atatcttgcc
     1501 tgttgcaggg agcgcagtac caagcggctg ataactgctt cctctggctt tccttcaata
     1561 tcaaaaagta tcttttgtaa atcctttgga tgaactttgc tggcaccttt gatacgatag
     1621 ccgaaattgt gaataaaact tgataaagtc aggatttttt ctgaatccgg atcttcatga
     1681 gtacgataaa taaaaggaac acctctccaa taaaaatctt ctgcaatcgt ttcattacac
     1741 actagcataa actcttcaat aattctggta gcaatatttc tttctacgat ttgtacatca
     1801 acagcctttc ctttttcatc taaaataatt tttgcttctt cgaaattaaa atcaattgcc
     1861 cctcttttaa tcctcttttc cctcagaata atacacaact cttccatgag tttgaaaaag
     1921 tcaacgtaat ctttatattt gctgataagt tcttcatctt tgtccactaa aattttcttt
     1981 acatttgtat aagtcatccg ttcattgaca ttaataacag attccacgac tctatggttt
     2041 tttactacac ccgcactgtc tatatccatg atacagctaa gtgctaacct gtcctcccct
     2101 gcatttaaag aacatattcc attggaaagc ttatgaggaa gcatagggat tactctgtcc
     2161 accaaataaa cactggttcc ccttttataa gcttctttat ctaaagctgt attttctctt
     2221 acataatggg atacatctgc aatatgaact cctaaacggt ataaaccatt gggtaacttt
     2281 tctaaagaaa tggcatcatc caaatccttt gcatcttcac catctatggt aaccattctt
     2341 atagctctta agtcttctct tcccttgata tcctcttcac tgagttcatc tggaacagat
     2401 tctatttctt tcatgacttc ttcaggaaag tccataggca tgtcaaattc tttaataata
     2461 gataaaatat ccgttccggg atcattgata tgacccagaa tttctatgat ttcgccttct
     2521 ggatttcttc tgtccttacc ccagtcggtt attttaacaa ctactttatg ccctgtaaca
     2581 gcgcctaaat cgtctccttt aggaataaaa atatctttgg aaaagttttt tccatcagga
     2641 atgacaaaac caaaattctt acttttctcg tatgtaccta cgactttctc tgaacctctt
     2701 tcaagaatct gtacaatttc cccttctgcc tttcttccgc catgctctgc ttttataatt
     2761 ttgcataaga ctttatcctt atgcatagca ccattgacct tatcggcagg aataaagata
     2821 tcattggcat cactgtcttc taacagtaaa aatccataac ctttagcatt accctgaaaa
     2881 atacctatta ccatgtttaa agtttcgggg agggaatatt tatctttttt ggtgcggata
     2941 atttttcctt cctgaatgag ctggtttaag atctcttcca aagcagctct ttcatgattt
     3001 ggtacttgta acaacatact aagctcttta attttcatcg gtctgtaatc tttatgactc
     3061 attaaatcca gaatcaattg ttttctttct tccatttttt ctattgtatc caatccagtc
     3121 acctcgtata aaaaactatt tatctttagt atatcaattc ttaaaaaaag taaacataaa
     3181 ataaaacaaa acacacttat tttcaaagtg tgttatttta atgtcttatt taattaatac
     3241 aactaaaaca acagcaagaa caataaataa tactgcaagg attttagtaa acttttcaag
     3301 ctgaccttct aaggaatacg ctttattttt tccccaaaaa gtatctgaat ttgatccaga
     3361 aagagtacct aaacctgcag attttccttc ctgaagcatt acagccgtaa taagaattaa
     3421 agccaatatt acataaacta ctgataatac aatcgttagt gtgctcattt ctacccctcc
     3481 taagttttta acatcaacat tttagcataa agtaataaat tgtgcaataa aaataagcaa
     3541 atact
//
LOCUS       DAOSUD010000136         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10891_length_3523_cov_24.126874, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000136 DAOSUD010000000
VERSION     DAOSUD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10891_length_3523_cov_24.126874"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..223
                     /locus_tag="PLG49_10680"
     tRNA            150..223
                     /locus_tag="PLG49_10680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Met,seq:cat)
     gene            237..310
                     /locus_tag="PLG49_10685"
     tRNA            237..310
                     /locus_tag="PLG49_10685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:271..273,aa:Pro,seq:cgg)
     gene            515..3034
                     /gene="pbp4b"
                     /locus_tag="PLG49_10690"
     CDS             515..3034
                     /gene="pbp4b"
                     /locus_tag="PLG49_10690"
                     /inference="COORDINATES: protein motif:HMM:NF002968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HOQ17810.1"
                     /translation="MMKKKSIFLSGFLVLFIITVLLVFSNNKIIKSFYSAPHDEELTD
                     ISDNILEGSESKEEDFDTEYLSYIDTFIESEIDKGFPGAVLLVMKDGKVVKHTAYGYK
                     LKYENGIELSNPIAMTEDTLFDLASNTKIYATTLAIIKLVDEGRIHLTDPVEKYLPLF
                     KDKETDPIKMKKYVTIEHLLNHSSGLPANFKFYDPKSAKDFYSLDREKTIQYLMEIPL
                     EYEPGTKDVYSDIGFMILGVIIEEVTGQSLDQYVEEKIYAPLGLKRTVFCPLKKGFKK
                     EEIAATELNGNTRDFNIDFPNIRTYTLQGEVHDEKAYYSMGEVAGHAGLFSTAKELGI
                     LSQMILNGGKFGDYYLADEETIRTFMEPSAVTSFRTLGWNRASWEGQENKFGEYAGPD
                     TIGHTGWTGTFTHIDPEKNIIVILLTNKKHSPVTYTDGGKIDGFKGDYFETGKYGKIL
                     TMVYNAYYKKHPEERPPVRLGIDRINQYQTLFQGKKIGLITNHTGMNSQGEVSIDVLY
                     EKFNLIALFSPEHGIRGNQEAGTSVEHSIDEKTGLPIYSLYGQTKRPTEEMMKDIDIL
                     AFDMQDVGARFYTYISTMAYAMEACAQYDKTFVVFDRPNPIGGKVEGNCLDSSYWSFI
                     GVYSIPQRHGMTVGELARLFNEEYGIGCKLVVIPMTRYSRDLYYEECGLNVWQPPSPN
                     MPAVDTALVYSGTCIFEGTNISEGRGTDKPFEIIGAPWINGEVLADEMERLNLPGVQF
                     RAITFVPAENKYKGQSCEGVELIITDRKQYNSVLTGLYLLNTIQKLYPEKIEYRNEHF
                     DALTGEPLLRSGNYSVEEIEWQWIEEARDFAEMSKKYYLYQ"
     gene            complement(3096..>3523)
                     /locus_tag="PLG49_10695"
     CDS             complement(3096..>3523)
                     /locus_tag="PLG49_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17811.1"
                     /translation="ICKNNPDLAKTKKQLKIILVSSIILYALLIVTEIIFYGFEARTF
                     LKSFLNLCVISLFSIGIMNGLRVFALLLSISSLRSLLSLVISIFKFDSIHSSHIIYLL
                     LFGLIHIILLVLYLYIVFSPKMQDYELALRSITYTDTHQ"
BASE COUNT         1200 a          493 c          730 g         1100 t
ORIGIN      
        1 ttttggcctt gcttttttgt ttcactcata gagcagtaca gttcatttat taagaaacaa
       61 taaaccgttg acaatcaact aaatctcatg ctattatatt taagcaattg actaagtagt
      121 ttgttatttt tataaaagaa tatgtatatg gcggagtagc tcagttggct agagcatacg
      181 gttcataccc gtagtgtcgg cggttcaaat ccgtcctccg ctattcattt tttttacggg
      241 gtgtggctca gtttggcaga gcgcttggtt cgggactaag aggtcgcagg ttcaaatcct
      301 gtcaccccga ttattgcctg ttggttattc ttaactgaca ggcatatttt ttttattaag
      361 ctcgtagcca tattaaagat agactgatag aaaaggatca gcgcggatct ttggtacttt
      421 tcgcttggaa aacagtaaaa tttgtagtat tatggaaaca gtaaggtaga aaatcagctt
      481 tttttgatat ttaatagcat agaagggata tattatgatg aaaaaaaaga gcattttttt
      541 atcaggcttt ttagttttgt ttattataac tgtcttactt gttttttcaa ataataaaat
      601 aataaaaagc ttttattctg ctcctcatga tgaggagcta acagatatat cagataatat
      661 tttagaagga agtgaatcca aggaagagga ttttgataca gaatatttat cttatattga
      721 tacttttatt gaatctgaaa tagataaggg gtttccaggg gccgttttgc tggttatgaa
      781 agatggaaaa gttgtaaaac atactgctta tggatacaaa ttaaaatatg agaatggaat
      841 agaattaagc aatcccatag ctatgactga ggatacttta tttgatttgg catccaacac
      901 aaagatttat gcaacgacat tggccatcat aaagttagtg gatgaaggca gaatccattt
      961 aacagatcca gtagaaaagt atttgcctct ttttaaagat aaagaaacag atcccattaa
     1021 gatgaaaaaa tatgttacta tagaacatct attaaatcac agctccggat tgcctgccaa
     1081 ttttaaattc tatgatccaa aatcggcgaa agatttttat tctcttgaca gggaaaaaac
     1141 aattcaatat cttatggaaa ttcctcttga atatgagccc ggtactaaag atgtctacag
     1201 cgatattggt tttatgattt taggggtaat tatagaagag gttacaggtc aaagcctgga
     1261 tcaatatgtt gaagaaaaga tttatgcacc actgggactt aaaaggacag tattttgccc
     1321 tttaaaaaaa ggttttaaaa aagaggagat tgctgctacg gagcttaatg gaaataccag
     1381 agattttaat atagatttcc ctaatataag aacttatact ttgcaagggg aagttcacga
     1441 tgagaaagcc tattacagta tgggggaagt tgcaggtcat gccgggcttt tttctacggc
     1501 aaaagagctg ggaatactgt cgcaaatgat tttaaatgga ggaaagtttg gagattatta
     1561 tctggctgat gaagaaacaa ttcggacttt tatggagcct tctgctgtaa catcttttag
     1621 aactttaggc tggaacaggg cttcatggga gggacaagaa aataagtttg gtgaatatgc
     1681 aggtcctgat accattggtc atactggctg gacaggtact tttacccata ttgatcctga
     1741 aaaaaatatc attgttattt tactgaccaa taaaaaacat agtccagtga cttatacgga
     1801 tggcggaaag atagatggtt ttaaaggaga ttattttgaa acgggaaaat atggaaagat
     1861 tttaactatg gtctataatg cttactataa aaaacatcca gaggaaagac cgccagttcg
     1921 tttaggaatt gacagaatca atcagtatca gacacttttt caaggcaaaa aaataggctt
     1981 gattaccaat catacgggaa tgaacagcca gggggaggtc agcattgatg ttttatatga
     2041 aaagtttaat ttgatcgcat tattttctcc agaacatggt atcagaggta atcaggaagc
     2101 agggacaagt gttgagcaca gtatagatga aaaaacgggt ctgcccattt atagtttgta
     2161 tggacaaaca aaacgaccga cagaagaaat gatgaaagat atagatattt tggcttttga
     2221 tatgcaggat gtcggggcaa gattttatac ctatatttcc accatggctt atgctatgga
     2281 ggcttgtgcc caatatgata aaacttttgt agtttttgac aggccaaatc cgattggtgg
     2341 caaagttgaa ggtaattgct tagattcttc ttattggtct tttattggtg tttattccat
     2401 tcctcagcga cacgggatga cagtaggaga attagcaaga ctatttaatg aagaatatgg
     2461 aataggttgt aagcttgttg ttattccaat gactcgctac agtagggatt tatattatga
     2521 agaatgtggt ttgaatgtct ggcagccacc ttctcccaat atgcctgcag tggatacagc
     2581 tttagtttat tcagggacct gtatatttga aggaaccaat atttctgaag gaaggggaac
     2641 ggataagcct tttgaaatca tcggggcacc ctggattaat ggagaagttc tggcggatga
     2701 aatggaaaga ttaaatttgc ctggtgttca atttagggct attacctttg ttcctgcgga
     2761 aaataaatat aagggacagt cctgcgaagg agtagagctt attataacag acagaaagca
     2821 atataactca gtgctgacag gtttgtattt attgaataca atacaaaaat tatatcctga
     2881 aaaaatagag tatagaaatg agcattttga tgctttaaca ggcgaaccgc tacttcgttc
     2941 gggaaattat tctgttgaag aaatagaatg gcaatggata gaagaagcca gggattttgc
     3001 agagatgtca aaaaaatatt atttatatca atagcctatc agagtttatc tgataggcta
     3061 tttaatatga aaagaatttt aaataaaaaa attaattatt gatgcgtatc tgtgtaagta
     3121 attgatctta atgcgagttc gtaatcttgc attttagggc taaagacaat ataaagatac
     3181 agaacgagta aaatgatgtg aataagcccg aaaagaagta aataaatgat atggctgctg
     3241 tgtatagaat cgaatttaaa aatgcttatc actaaactta acaaacttct taaagagctg
     3301 atggaaagaa gaagggcaaa tactcttaaa ccattcatga ttcctattga aaataaggaa
     3361 ataacacaca aatttaaaaa agactttaaa aatgtccttg cttcgaatcc ataaaaaatg
     3421 atctctgtaa cgattaatag agcatagagg ataatagaac ttacaagtat tatttttagt
     3481 tgttttttag ttttagctaa gtcaggattg ttcttacata tat
//
LOCUS       DAOSUD010000137         3508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_10948_length_3508_cov_28.860411, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000137 DAOSUD010000000
VERSION     DAOSUD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10948_length_3508_cov_28.860411"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLG49_10700"
     CDS             complement(<1..904)
                     /locus_tag="PLG49_10700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ17812.1"
                     /translation="MTEENIKITPAKGRPMLNWVGKRPLDYVKGFPAQLVECFDPLNV
                     NKIPQTPNYESLKDNWHNLLFHGDNKDVLATLLEKGFRGKIDLIYIDPPFKSGADYVR
                     KVELRGVKNSNKLDNDDASILQQTMYFDIWNNDNYLQFMYERLLLLKELLSETGSIYV
                     HLDSYIGHYIKILMDEIFGQGNFRSSIIWNTSVPQVAGFKWLSNGWIYSQAMILYYTK
                     SKTNYVFNKQYIEVKQKSGDISKKPISDIWNDIKNFAGIFGAKDSKTGFQTQKPEALL
                     ERIIKASSNPDDIVLDCFIGSGTTA"
     gene            complement(910..>1571)
                     /locus_tag="PLG49_10705"
     CDS             complement(910..>1571)
                     /locus_tag="PLG49_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17813.1"
                     /translation="NHSNELKKQLEGQIKNYEIIEEVIEQSIAIVKKEGFIKEERENN
                     ELVYTAEIIYHKDKAEKLFAYYEDLKKINSLDFSFHYNPYNFDSQPEKDFFINLLEDL
                     NQNPDDVEDIYFTGAITDPNKTDFFFEYKGVDERWHTYTPDFLIRKKDGKIIIVEIKM
                     EKLKGNNIDGENGLKATAVKNIQHLNPDKIKFEMLFTDSDDIGISNLKKIKEIIYKQW
                     R"
     assembly_gap    1572..1671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1672..3201)
                     /locus_tag="PLG49_10710"
     CDS             complement(<1672..3201)
                     /locus_tag="PLG49_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOQ17814.1"
                     /translation="MKKELFIPNKLKEEVSKWRETSYDCDYPVISEILDYNVVISNSG
                     EKSLRFLRQPQFDALEVYWYLRIVKDTPHVLDIYKNLYSSKELLEALGLKHLVEDLTD
                     DLVSETLVNDILNKVKYDDNFVKEHKLESLRETLTLNYPSYIFALAMGAGKTVLIGAI
                     IATEFAMALEYQDNKFVKNVLVFAPGKTILGALRELSDVPYEKVLPPRLYKEFITSVK
                     FTYTRDGESDIPIIKGSSFNIIVTNTEKIRIQKQAITKAILKDIKIPFSNNDESKKDQ
                     IKEEVANLRLQTIASLPNLAIFSDEAHHTYGNTLDKGLKKVRKTVDYLAYNTNVLVVV
                     NTTGTPYYKKQVLKDVVFWYGLSQGIKDGILKEVRGNIVGYEEVSDEEFIKDVVNDFF
                     DNYENVKIYDGSPAKLAIYFPKVDDLIKSKPIVEKTLVERGLDPSIILEVCNESPDEV
                     KDLFDNRINDSKLQYRVFLLVNKGTEGWNCLSLFATALARKIKSSNNFCLQAATRCLR
                     QI"
     gene            complement(3229..>3508)
                     /locus_tag="PLG49_10715"
     CDS             complement(3229..>3508)
                     /locus_tag="PLG49_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17815.1"
                     /translation="WDIYNSLKHSCYPLESHFVLFTKDIIGEKHEMLLYIFDGNEKYY
                     LPIWGGWVAPEGAEDYAKSIENPFITLIKAIGNDIIDNLKTLNEILTI"
BASE COUNT         1063 a          595 c          417 g         1333 t
ORIGIN      
        1 cagctgtagt tcctgaacct ataaaacaat ctaaaacaat atcatcggga ttagaggaag
       61 cttttataat tctttcaagt agcgcttctg gtttttgagt ttgaaaacct gtttttgaat
      121 cttttgctcc aaaaataccc gcaaaatttt ttatatcatt ccatatatcg ctaatcggtt
      181 tttttgaaat gtctcctgat ttttgtttaa cttcaatata ttgtttgtta aaaacataat
      241 ttgtttttga ttttgtataa taaagaatca ttgcttgtga atagatccag ccatttgata
      301 gccatttaaa acctgccacc tgtggaacag aagtattcca tattattgaa cttctaaaat
      361 tcccctgacc aaatatttca tccattaaaa ttttaatata atgccctata tatgaatcga
      421 gatgcacata aatgcttcca gtttcactca ataattcctt taataataat aatctttcat
      481 acataaactg taaataatta tcgttgttcc atatatcaaa atacattgtt tgttgtaaga
      541 tagaagcatc atcattgtct aacttatttg aatttttaac tcctcttagt tctacctttc
      601 tgacataatc agctccgctt ttaaaaggcg gatctatata tataagatct attttacctc
      661 taaaaccttt ttctaagaga gttgctaaaa cgtctttgtt atctccatga aataaaagat
      721 tatgccaatt atctttaagg ctttcataat taggtgtttg gggaatttta tttacattaa
      781 gaggatcaaa acattctact aattgagcag gaaaaccttt gacataatcc aaaggtcttt
      841 taccaaccca gttaagcata ggtcttcctt tagcaggcgt tattttaata ttttcttcag
      901 tcattcagtt tacctccatt gcttataaat tatttctttg atttttttca aattactaat
      961 tccaatatca tcactatcgg taaatagcat ctcgaatttt attttatcag ggtttaagtg
     1021 ttgaatattt ttaactgcag tagcttttag accattttcc ccatctatat tattgccctt
     1081 tagtttctcc atttttattt ctacaatgat tattttccca tctttttttc ttattaaaaa
     1141 gtcaggtgta taagtatgcc atcgttcgtc gacaccttta tactcgaaaa agaaatcagt
     1201 cttattagga tcagttattg caccagtaaa ataaatatct tccacatcat caggattttg
     1261 attgagatct tctaaaagat tgataaaaaa atctttctct ggttgtgaat caaagttata
     1321 cggattataa tgaaaggaga aatctaaact attaatcttt tttaaatctt cataataagc
     1381 aaataacttt tcagccttat ctttatggta aattatttct gcagtatata ctaattcgtt
     1441 attttctctt tcttctttaa taaatccttc tttttttaca atagctatac tttgctcgat
     1501 tacctcttct attatttcat aattttttat ttgtccttct aattgctttt tcaattcatt
     1561 agaatgatta annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatttgtct
     1681 caaacagcga gttgcagctt gtagacaaaa attattactt gatttaattt ttcttgctaa
     1741 agcagttgca aatagtgata aacaattcca tccttctgtt cctttattta caagaagaaa
     1801 cacacgatat tgaagttttg aatcatttat tctattatca aaaagatcct ttacttcgtc
     1861 tggtgattca ttgcacactt ccaaaattat tgaagggtcc aaccctcttt caaccaatgt
     1921 tttttcaaca ataggtttag attttattaa atcatctact tttgggaaat aaatagctag
     1981 ttttgctgga cttccatcat atatttttac attttcataa ttgtcaaaaa aatcattaac
     2041 aacatctttt atgaattctt cgtcactaac ctcttcatag ccaacaatgt ttcctctaac
     2101 ctcttttaaa atcccatctt taatcccttg agataaacca taccaaaaaa ctacatcttt
     2161 caatacttgt ttcttataat aaggcgttcc cgtcgtatta acaacgacca agacgtttgt
     2221 attgtatgcc aaataatcaa ctgttttcct tacttttttt aaacctttat caagagtgtt
     2281 tccgtatgta tgatgggcct catcagaaaa gatggctagg tttggaagcg aggcaatggt
     2341 ctgaagtctt aaatttgcta cctcttcttt tatttgatct tttttgcttt catcattatt
     2401 agaaaaaggt atttttatat cctttaatat agcctttgtt atagcttgtt tttgaattct
     2461 tattttttcc gtattagtaa ctattatatt aaaactactc cctttaataa taggaatatc
     2521 tgattctccg tctcgtgtat aagtgaattt tacagaggtt ataaattctt tatacaatcg
     2581 tggaggcaag actttttcat aaggtacatc tgataattct cttaaagccc ctaaaatagt
     2641 ttttccggga gcaaaaacta aaacattttt tacaaacttg ttgtcttgat attccagagc
     2701 catggcaaat tccgtcgcta tgatggcacc aataagaact gttttaccgg ctcccatggc
     2761 taaagcaaaa atatagctgg gataattcag agttaaagtt tctctcaaag attccaattt
     2821 gtgttcctta acaaaattat cgtcatattt tactttattt agaatatcat tgactaaagt
     2881 ttctgatacc aagtcatctg taagatcttc tactaaatgt tttaatccta atgcctctaa
     2941 aagttctttg cttgaatata aatttttata tatatctaat acgtgcggag tatcttttac
     3001 tattcttaaa taccaataaa cctccaaagc atcgaattgt ggttgcctta aaaatctcaa
     3061 acttttttct cccgaattac taataacaac attataatct aatatttctg atataacagg
     3121 atagtcacaa tcatagcttg tttctcgcca tttgcttact tcttctttca atttatttgg
     3181 tataaataat tcttttttca tttcaacacc tctacttaga ctttagattt aaatagtcaa
     3241 aatctcatta agcgttttta gattatcaat tatatcgttc cctatggctt ttattagtgt
     3301 aataaaaggg ttttctatac tttttgcata atcttcagca ccttcaggag ctacccatcc
     3361 accccaaata gggagataat atttttcatt tccatcaaat atataaagca acatttcatg
     3421 tttttcgcct attatatctt tggtaaaaag gacgaaatgt gattcgagag ggtaacatga
     3481 atgttttaaa gaattataaa tatcccat
//
LOCUS       DAOSUD010000138         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_11066_length_3481_cov_22.920607, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000138 DAOSUD010000000
VERSION     DAOSUD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11066_length_3481_cov_22.920607"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1031
                     /gene="era"
                     /locus_tag="PLG49_10720"
     CDS             126..1031
                     /gene="era"
                     /locus_tag="PLG49_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ17816.1"
                     /translation="MALDFRSGFVTLIGRTNVGKSTLMNQLVGEKVAIMSNKPQTTRN
                     KIQSILTRENYQIIFIDTPGIHKPKHKLGQYMVQAAKNTLNEVDLILILVEASKTVGK
                     GDQYVIEQLENINTPVFLVINKIDTVHKEELLEIIDIYKDLYDFDEIIPVSALTGENC
                     DVLLDEISKRLPEGPQYFPPDMITDQPERQLVAELIREKALHLLEEEIPHGIAVGVEL
                     MKKRENKDIVDINAVIYCEKDSHKAIIIGKQGAMLKEIGTKARQEIERLLGSKVFLQL
                     WVKVRKNWRDDSFSLRNFGFDSKNI"
     gene            1086..2441
                     /gene="mgtE"
                     /locus_tag="PLG49_10725"
     CDS             1086..2441
                     /gene="mgtE"
                     /locus_tag="PLG49_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ17817.1"
                     /translation="MEKERLLENIKTALQNKDSRVLFEEIDELHPADIVEILPDLEEQ
                     EQKDLFKLLPHDVAAEVLNAVDGDTFAYILDNLTEEKTSEILDEMSLDDVADILGELS
                     ESEQTKIIEFFDHDDATDIRDLMQYHEDTAGGIMTTEFVSVREDMTINQAIEELRSME
                     KKAETIYYVYVTDYIGVLVGVLSLREIIMSPPDLLVSEVMQSNVIYVNVETDQEEVAK
                     LVSKYDLLAIPVVDNETILQGIITVDDILDVIEEEATEDIFRIAGTGEMEYDESDSLA
                     GHLKSSVKSRLPWLLITLVGSFVTGAVISGFESQISSNVALVKFMPALTGMGGNIGTQ
                     SSTLTVRGIATGKIDTKEVSKILLRESAVGIFIGFICSICISLVAGFVFKQPQLGPIV
                     GIAMTTNMLTAATIGTLAPLIFKNIGIDPAVASAPFISTTIDITGNIIYFSLVKLLTG
                     I"
     gene            2548..2718
                     /locus_tag="PLG49_10730"
     CDS             2548..2718
                     /locus_tag="PLG49_10730"
                     /inference="COORDINATES: protein motif:HMM:NF025372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HOQ17818.1"
                     /translation="MTDKLFWNLFKRTGNIEAYLALKEYNKVMTNMDIESSKELSPEI
                     GHKDKKSDGKEK"
     gene            2721..3473
                     /gene="recO"
                     /locus_tag="PLG49_10735"
     CDS             2721..3473
                     /gene="recO"
                     /locus_tag="PLG49_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ17819.1"
                     /translation="MRQFKTKGIILREVYVGESDKILKIFTKLKGKISVSAKGSRKNK
                     SPLAAGSQLFSYSDFLIYSGRNYNIIQQIDIIKTFHGIGKDIIKLTYASYFLELLDGV
                     TEEEETNEELLLLILKTLSVLEKSNRNPRLISVIYELRLMSLIGYMPQVLECVNCGSK
                     EKIHHFNIKMGGVLCDDCMHEDYYAYKLSQSGWYTLKYIIHSDLSKLFSFDLDEFILK
                     ELEIISEKYLSYHIEKDFQTLEFIKTNLMDLQ"
BASE COUNT         1244 a          464 c          713 g         1060 t
ORIGIN      
        1 ataatataat aaagattggt ttaacttttt gattgttatg gaatgccatt aaaaatggag
       61 gaaacgtatt tatataaaat agcaataaag gataatataa ataaaagaga aaaggaggaa
      121 tagcattggc attagatttt cgttcaggat ttgtaacgct tataggaaga acgaatgtag
      181 gaaaatcgac cttaatgaat caactggttg gagaaaaagt ggccattatg tccaataaac
      241 ctcagacgac cagaaataaa atccaaagta tcttaacaag agaaaactat cagattattt
      301 ttattgatac gcccggtatt cataaaccca agcataaatt aggccagtat atggtacagg
      361 cagccaagaa tacgctcaat gaagttgatt taattttaat ccttgtggaa gcttcaaaaa
      421 cagtgggcaa gggagaccag tacgttattg aacaattaga aaatatcaat acacctgtgt
      481 ttttggtgat taataaaatt gataccgttc ataaggaaga attgttagaa attattgata
      541 tttacaagga tttatatgat tttgatgaga tcattccagt gtcagccctg acgggagaaa
      601 actgcgatgt tcttcttgac gagatatcca aaaggttacc tgaaggacct caatattttc
      661 cgccagatat gattacagac cagcctgaaa gacaactggt agcagaatta atccgggaaa
      721 aagctttaca tttgttagaa gaagaaattc ctcatgggat tgcggtggga gtagaattaa
      781 tgaaaaaaag agaaaacaaa gatatcgtcg atattaatgc agtgatttat tgtgaaaaag
      841 attctcataa agccattatt attggaaagc aaggtgctat gttgaaagaa atcggaacaa
      901 aagcccgcca ggaaatagaa agacttttag gatccaaggt gtttttacag ctttgggtaa
      961 aggttagaaa aaattggaga gatgattctt tcagcttaag aaattttggt ttcgattcaa
     1021 aaaatatttg aaaatgcagc agcgctataa tacaataaaa tagtgtaaag aaatggggtg
     1081 aaggtatgga aaaggaacgt ttgctggaaa atattaagac tgctcttcaa aataaggata
     1141 gcagggtatt atttgaagag attgatgagc tgcatcctgc tgatatcgtt gaaatattgc
     1201 cggatttaga agaacaggaa caaaaagact tgtttaaact tctcccccat gatgttgctg
     1261 cggaagtttt aaatgcggtc gatggagata cttttgcata tattttagat aatcttacgg
     1321 aagaaaagac cagtgaaata ctggatgaaa tgtccttaga cgacgtagcg gatattttag
     1381 gagaattatc tgagagtgaa cagacaaaaa ttattgaatt ttttgatcat gatgatgcaa
     1441 cggatatccg tgatttgatg cagtatcatg aagatactgc cggtggaatt atgaccaccg
     1501 aatttgtttc tgtaagagaa gatatgacca ttaatcaggc tatagaagaa ttaaggagta
     1561 tggaaaaaaa agcagagacc atatactatg tttatgttac agattatatt ggtgtactgg
     1621 taggggtctt atctcttagg gaaatcatta tgagtccacc tgatttactg gtctctgaag
     1681 tcatgcagag taatgtcatt tatgttaatg tagaaacgga ccaggaagaa gttgctaaac
     1741 tcgtttcaaa atatgactta ctggccattc ctgttgttga taacgagacg attttacaag
     1801 ggattattac cgttgacgat atccttgatg ttattgagga agaagcaacg gaagatattt
     1861 ttagaattgc cggtactggt gagatggaat acgatgagag tgacagtctg gctggtcact
     1921 taaaatcttc agtaaaatca aggcttccat ggcttctgat tactttagtt ggaagttttg
     1981 tcacaggtgc tgttatttca ggctttgaaa gccagatcag cagtaatgta gccctggtta
     2041 agtttatgcc agcgctgaca ggaatgggag gaaatattgg tactcagtcg tcaaccctta
     2101 cggtaagggg aattgccaca gggaaaattg atacaaaaga agtatcaaaa attttacttc
     2161 gtgaatctgc cgtagggata tttattggtt ttatatgtag tatttgcatt agtttagtag
     2221 caggttttgt tttcaaacaa cctcaattag ggcctattgt aggcattgcg atgacaacca
     2281 acatgttaac ggctgctacc attggaactt tagcaccact aatatttaag aatataggaa
     2341 ttgaccctgc ggttgcttcg gcgccattta tttcaacgac gattgatatt acaggaaata
     2401 ttatttattt cagcttagta aaattattaa ctggaatata aaatagattt ttgtaaatag
     2461 atgatgaact ggtaactttt tttgcagtat attaagagaa gttgagggaa ccctaatatt
     2521 aacaaattag gaataggggg actcaatatg acggataaac tattctggaa tttatttaaa
     2581 agaacaggaa atattgaagc atatttagct ttaaaagagt ataataaagt aatgactaat
     2641 atggatatag aaagctcaaa ggaactgtct ccagaaatcg gccataaaga taaaaaatca
     2701 gatggaaaag agaaataatc atgagacaat ttaaaacaaa aggaattata ttaagagaag
     2761 tttatgtggg tgagtcagat aaaatactca agatatttac aaaattaaaa gggaagattt
     2821 ctgtttctgc aaaagggtcc agaaaaaaca aaagcccttt ggcagcaggt agtcaattat
     2881 tctcttattc tgatttttta atatattcag gcagaaatta caatattata caacaaatag
     2941 atattattaa gacatttcat ggcataggta aagatatcat taaattaacc tatgcttctt
     3001 attttttaga acttcttgac ggggtcacag aagaagaaga aaccaacgaa gaacttttgc
     3061 ttttgatttt aaaaactctc tctgtgcttg aaaaaagtaa tagaaatccc aggctgattt
     3121 ctgttattta cgaacttaga ttaatgtctt taattggcta tatgccacag gttttagaat
     3181 gtgtcaattg tggcagcaag gaaaagattc atcattttaa cataaagatg ggcggggttc
     3241 tttgcgatga ttgtatgcat gaagattatt atgcttacaa gctttcgcaa tcaggctggt
     3301 atacgctgaa atatattatt cattctgact tgtcaaaact atttagtttt gatttagatg
     3361 aatttattct aaaagaattg gaaataatct cagaaaaata tttatcttat catattgaaa
     3421 aagattttca gactttagaa tttatcaaga caaatttaat ggatttgcag taaatcatta
     3481 t
//
LOCUS       DAOSUD010000139         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_11626_length_3346_cov_23.366150, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000139 DAOSUD010000000
VERSION     DAOSUD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11626_length_3346_cov_23.366150"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..592)
                     /locus_tag="PLG49_10740"
     CDS             complement(176..592)
                     /locus_tag="PLG49_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOQ17820.1"
                     /translation="MKSYRKELYFNTPTRRAFINITPQIEECLRESGIKEGLLLCNAM
                     HITASVFINDDEPGLHRDFERFLEKLAPEKPYDQYDHNGFEDNADAHLKRTIMGREVV
                     VAITDGKLDFGPWEQIFYGEFDGKRTKRVLVKIIGE"
     gene            complement(594..950)
                     /locus_tag="PLG49_10745"
     CDS             complement(594..950)
                     /locus_tag="PLG49_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17821.1"
                     /translation="MSKKILAIVALAEKLLSSEEKVDWDKELDFFARWIGYFQHERLV
                     HLIVTCLFSLLTILSMMINLYSSSLATLILFLLFFILLIPYIGHYYLLENKTQYLYDL
                     YDKMIEKAYEAKKGGN"
     gene            complement(989..3124)
                     /locus_tag="PLG49_10750"
     CDS             complement(989..3124)
                     /locus_tag="PLG49_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ17822.1"
                     /translation="MQNGLVLNDYIVCPKCNYRLSLKKIGIIFCSSCNQLIEVKMDKD
                     KNLIISADILFKNGVRFKCLGIRENGYARGEVLIPNKFRFVGECICEHGKIVKGKGKI
                     TYVNNDKYNGEIVDDTPHGNGIMIWKNGDAYTGEWKEGLPCGNGIMTYETGARYEGSF
                     KDGLPNGYGIMTWKNGSWYKGNFKNKMYHGQGKYFDISNVIEEGEFNNNKFWKGIRYE
                     KISDRWMKITIENGKPVSSEPCEGPVLDEEASSIYELVKELKARLKECNKIAAYLEHY
                     YKDQNQLQGLNIFKEIVQHIGIIIIQVIASNREVCFSHVRLFNEVFGFKYTKEEFETF
                     VNSVLMDWSKKYIGDIPYYFELCLNKDVETGTNDSRILFSHYNFFALSFLSIDHKIDY
                     VEMDFITDYFMKIERLLNEKKIRPLENGEYYLKGKTNEKSLENYFDELNALIGLQKVK
                     QDVRTLVNFIRVQKLRKERNLPVPNMSLHLVFTGNPGTGKTTVARILAAIYKEIGVLT
                     KGHLIEVDRSGLVGGYVGQTAIKTKEVIEKALDGVLFIDEAYTLASSSEQDYGQEAID
                     TLLKAMEDYRDRLIVIVAGYPDPMEKFLSSNPGLRSRFNKYIHFDDYSADELLQIFEL
                     IVKKNGYILLNEVREYMRKVFEDLKHKAETNIDPSYHFSNGREVRNIFEKLVSIQANR
                     ISSGFVTDTELMTITLEDCKQLFERIKEN"
BASE COUNT         1071 a          639 c          380 g         1256 t
ORIGIN      
        1 ataaatcgac ctagttcgat tttttgtatt atatttttac tttggtatat attattttac
       61 aatatcatcc tcaataattc aatcttttaa tagtttaaga ataactttta acaatttttt
      121 taattaaaaa actcatgaat ctttgaaaag attcatgagt tttaagaaaa gttttttatt
      181 ctccaatgat tttaactaat actcttttcg ttctttttcc gtcaaattcc ccataaaaaa
      241 tttgctccca gggaccaaag tcaagttttc catcggttat tgctacaaca acttccctgc
      301 ccataatggt tcttttaaga tgggcatctg cattatcttc aaaaccatta tggtcatact
      361 ggtcataagg cttttctggt gctaattttt cgagaaatct ttcaaaatct ctgtgaagtc
      421 ctggttcatc gtcattaata aatacgctgg ctgttatatg catagcatta cagagcaaaa
      481 gtccttcttt aatcccactt tctcttaaac attcttctat ttgtggggta atattgataa
      541 aagctcttct cgtaggcgta ttaaagtata gttcttttct ataggatttc aaattaattt
      601 cctccttttt ttgcttcgta ggctttttca atcattttat catataaatc atacaaatat
      661 tgtgttttat tttcaagtaa ataataatga ccgatataag gaattaataa aataaaaaac
      721 aagagaaaaa gaattaaagt tgcaagggaa gaactataaa gatttatcat catactcaag
      781 attgttaaaa gagaaaataa gcaggttaca attaaatgaa caagacgctc atgttggaaa
      841 tatcctatcc agcgggcaaa aaaatcaagc tctttatccc aatctacctt ttcttctgat
      901 gataatagct tttctgctaa agcgacaatc gctaatattt tcttactcat aattttcccc
      961 catatattga ccccgtcaaa ttaatctttt aattttcttt tattctttcg aataattgtt
     1021 tacagtcttc taaagtaatt gtcatcagtt cagtgtctgt aacaaagccg ctgctaatcc
     1081 tatttgcctg tattgaaaca agtttttcaa aaatatttct tacttcccgg ccattactga
     1141 aatgataaga aggatctata tttgtttcgg ctttatgttt taaatcttca aaaacttttc
     1201 tcatatactc tcttacttca tttaataata tatagccatt tttcttcaca atcagctcaa
     1261 agatttgcaa gagctcatca gcgctataat catcaaaatg aatatattta ttaaatcttg
     1321 acctaagtcc tggatttgag ctaaggaatt tttccattgg atcaggataa ccggcaacaa
     1381 ttacaattaa cctgtctctg taatcttcca tggcttttaa taaagtatct atagcttcct
     1441 gaccataatc ctgttctgaa ctgcttgcca gagtataagc ctcatctata aataatacac
     1501 catctaaagc cttctcaatg acttctttgg ttttgatggc agtctgacca acataaccac
     1561 caactaagcc ggaacggtca acttctatca gatgcccttt ggtcaatact ccaatttctt
     1621 tataaatagc tgctaaaatt cttgcaactg tcgtcttacc tgtacctgga tttcccgtaa
     1681 aaactaagtg taaagacata ttaggtactg gaagatttct ttcttttctt agtttttgca
     1741 ccctaataaa gttaactaag gtacgaacat cttgctttac cttttgcaga ccaattaatg
     1801 catttaattc atcaaaatag ttttcgagac tcttttcatt tgtttttccc ttcaaataat
     1861 attccccgtt ttctaatggt cggatttttt tctcatttaa aagcctttca attttcataa
     1921 aataatctgt aataaaatcc atctcaacat agtcaatttt gtggtcaatg gataagaaag
     1981 ataacgcaaa aaagttatag tgggaaaata aaattcttga atcatttgta ccagtctcta
     2041 catctttatt caaacataat tcaaagtaat aagggatatc tcctatatac tttttactcc
     2101 aatccataag aacactatta acaaatgtct caaactcttc ttttgtatat ttaaaaccaa
     2161 atacttcatt aaataacctg acatgactaa aacaaacttc tctgttactt gctataacct
     2221 gaataataat gataccaata tgctggacaa tttctttaaa tatattcaag ccttgtagtt
     2281 gattttgatc cttgtaataa tgttcaagat aagctgcaat tttattacat tctttcagac
     2341 gggcttttaa ttcttttacc agttcataaa tgctgctggc ttcttcgtcc aatacaggcc
     2401 cttcacaggg ttctgaagat accggcttgc cattttcaat cgtaattttc atccatctgt
     2461 cacttatttt ttcatatcga atacccttcc aaaatttatt attattaaac tctccttctt
     2521 cgattacatt tgaaatatca aaatatttgc cttgtccatg atacatttta tttttaaaat
     2581 tccctttata ccaggaacca tttttccatg tcataatacc atatccattg ggcaaaccat
     2641 ctttaaaact accctcatac cttgctcctg tctcataagt catgatacca ttaccacaag
     2701 gtaagccttc tttccattca cccgtataag catcaccatt tttccatatc ataatgccat
     2761 ttccgtgtgg tgtatcatct acaatttccc cattatattt atcattattt acataagtaa
     2821 tttttccttt accttttaca attttcccat gttcacagat acattcccct acaaagcgga
     2881 acttattggg tattaaaact tctcccctgg cataaccatt ttctcttatc cctaaacatt
     2941 taaaacgaac accattctta aacaaaatat cagcagaaat aattaaattc ttgtccttat
     3001 ccatttttac ttctattaat tgattgcaac tggaacaaaa aatgatgcct atttttttta
     3061 aactcaaacg ataattacat ttgggacaga caatgtaatc attcaaaaca agcccatttt
     3121 gcatagtcat ttcctcctat aaaagggtct ttatgaaaaa cataattatt taccaatatt
     3181 catacttatg gaaacttgct ttttattata acatatatca taaagattat taatggattt
     3241 cagctttttt tgctttttta aaatattaaa ataatttata actttaacta caaaattgtt
     3301 acatttttta tcaattcatg ttataatatt ttatattttt atcatc
//
LOCUS       DAOSUD010000140         3339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_11655_length_3339_cov_22.912911, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000140 DAOSUD010000000
VERSION     DAOSUD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11655_length_3339_cov_22.912911"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="PLG49_10755"
     CDS             <1..2204
                     /locus_tag="PLG49_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated protein Csx11"
                     /protein_id="HOQ17823.1"
                     /translation="KIADILNRNEIIEDIKNNLTEKFEVKIPIGNVIYEDNNGIYFTF
                     PELNNNSELLAEECAKEALKIIYNISNNEIWPFFTLSRPSRSLTILAGELKFAAENRN
                     IPKMSPRLFIDGKEEKLLDSPDIPTPKDEDEDKNKVDICPVCKFRAKSIENERCDVCE
                     ERKIGRLNEWLSNRENTIWLDEVADTNNRIALLTLNFDLDKWLDGTMIGTIFSQTFED
                     WKNSNDAIKFFNNKQNTQKLEKQGIKIDDTFINLSKNLLKIITDENITKDPGFKSSII
                     NTFYEDIKSSQTEGDENYVRNFINNIKERIMPAQFNSCNLQKLIFTQNPSPARLYRIW
                     KETKEFFDLVTQNIKSNIYQNKWKRINFSVDYNELKSKLKQNATIEDNTPYIIKIKAL
                     TPENVLVFHSTNGEFYTIESLEKFRYGEKSGIEAIKVAFSNGINWISTEDAPDINLLQ
                     NDQNVKINNPIIEEYYYPFIEITKSPLSLRLLVPASDAIKILESIVKLYNQRFEKVIG
                     KLPLNMGLLVANRKFPLYVLLEAGGRMFGNKEFKDPDLMDVWWGINGLGNDEYYSFYP
                     MKKLDDHEYNLDDLSRLSKEKKFALYPGYFDFDSLLGTNDRYNIFYKDKKRASKDYKL
                     YSKRPYYFYQFAEMMDLWEILTNNISSSQINFIEEMFTNKLREWRNVSDANQEEVFTK
                     FAEATIKDAFSENWDNLRIETQDFILNSAYNGLLLDTIILFNHTIKGGQTK"
     gene            2201..3115
                     /gene="cmr4"
                     /locus_tag="PLG49_10760"
     CDS             2201..3115
                     /gene="cmr4"
                     /locus_tag="PLG49_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOQ17824.1"
                     /translation="MNEPKKYFGITLDPVYIGAGGYRLGMVDNTIVRDPATDVPKIPG
                     TSLAGAIREFATVYTMTNACQSENDEKEKRKYAEEEVKKYFGDENRQGMVRFYDGQII
                     FFPVSSIQGTVWLTTRDILEYWLGEIKDPQGEKIKIPDKIEDNAYAIKGIDSSKPLNL
                     GWLLLDTQNINGGKEITLPPELDKFLKRIVIVSDKLFSHIVNDNLEVRTSVRIDPETG
                     TAAEGALFTYEAIPRGTILGFEMVTDTRKGDNVESIVNLIEATFPYLKLLGLGGMGTR
                     GFGRVKFFTLKNNKQSNNKMEGNENDKS"
     gene            3102..>3339
                     /locus_tag="PLG49_10765"
     CDS             3102..>3339
                     /locus_tag="PLG49_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17825.1"
                     /translation="MTNLDYICAKIGQNLVVKADENIFRKALGILQEDGVYAMFLWLE
                     NEDSEIRKKLNSLLNENEIKSKLLSDSKSFPDDFK"
BASE COUNT         1297 a          485 c          609 g          948 t
ORIGIN      
        1 aaaaaattgc cgatattctg aatagaaacg aaattattga agatatcaaa aataacttga
       61 cagaaaaatt tgaagttaaa ataccaatcg gaaatgtcat ttatgaagat aataatggaa
      121 tctattttac attccctgaa ttgaataaca attcagaact acttgcagaa gaatgtgcaa
      181 aagaagcttt gaagattatt tataatatta gcaataatga aatatggccc tttttcacct
      241 tgagcaggcc atccagaagc ctcactatcc ttgctggaga attgaaattt gctgctgaaa
      301 atagaaatat cccaaagatg agcccaagac tttttattga tggaaaagaa gaaaaattat
      361 tagatagtcc tgatatacca actccaaaag atgaagatga agataaaaat aaagtagata
      421 tttgtcctgt atgtaaattc agagcaaaat ctatagaaaa tgaaagatgt gatgtttgtg
      481 aagaaagaaa aataggcaga ttaaatgaat ggcttagcaa cagagaaaat accatctggc
      541 tcgacgaggt tgccgacacc aacaatcgta ttgcattgct taccttgaac tttgaccttg
      601 ataaatggct tgatgggaca atgattggta caattttttc gcaaactttt gaggactgga
      661 aaaatagtaa tgatgctatt aagttcttca ataataaaca aaatacacaa aagttggaaa
      721 aacaaggtat aaaaattgac gatactttta ttaacttatc aaaaaatctt ttaaaaatta
      781 ttaccgatga aaatattaca aaagatcctg gttttaaaag tagtataata aatacgtttt
      841 acgaagacat aaaatcaagc caaacagaag gagatgagaa ttatgtaaga aattttatta
      901 acaacataaa agaacgcata atgcctgccc aattcaattc atgtaatttg caaaaattaa
      961 ttttcactca aaacccatct ccagctcgtt tataccgtat ctggaaagag acaaaagaat
     1021 tctttgattt agttactcag aatataaaat caaatatcta tcaaaataaa tggaaaagaa
     1081 taaacttttc tgtggattat aatgaattaa aatctaaact aaagcaaaat gcaacaatag
     1141 aagataatac tccttacata ataaaaatca aagccttaac gcctgagaat gttctcgtat
     1201 ttcattccac aaatggtgaa ttttatacca ttgaatctct tgaaaagttt agatatggag
     1261 aaaaatcagg cattgaggca attaaggtgg cttttagtaa cggaataaac tggatttcaa
     1321 cagaagatgc ccctgacatc aatctattac agaatgatca aaatgtaaaa attaataatc
     1381 caattataga agaatactat tatcccttta ttgaaattac aaaatcaccg ttatctctaa
     1441 gattacttgt accggcatca gatgctataa aaattctgga gtcgatcgta aaattatata
     1501 atcaaaggtt tgaaaaagta attgggaaat tacctcttaa catgggtttg cttgttgcga
     1561 acagaaaatt ccccctttat gtgcttctgg aagccggagg tagaatgttt ggaaataaag
     1621 aattcaaaga tcccgatttg atggatgtgt ggtggggtat aaatggactt gggaatgatg
     1681 aatattacag tttttatcca atgaaaaaac tcgatgatca tgaatataat cttgatgatc
     1741 tgtcccgctt atctaaagag aaaaaatttg cattatatcc cggatacttc gactttgatt
     1801 cgcttctggg taccaacgat agatacaata tcttctacaa ggacaaaaag agagccagta
     1861 aagattataa actctattcc aaacgacctt attattttta tcaatttgca gaaatgatgg
     1921 acttatggga aatattaaca aacaatattt ccagctcgca gattaatttt atagaagaaa
     1981 tgtttacaaa caaactgcga gaatggcgta atgtcagtga tgcaaaccag gaagaggttt
     2041 ttacaaaatt tgcagaggct acgattaaag atgctttcag tgaaaactgg gataatttaa
     2101 gaatagaaac gcaagatttt attttaaact ctgcatataa cggtctttta ctcgatacaa
     2161 taattttatt caatcataca ataaaagggg gtcaaacaaa atgaatgaac ctaaaaaata
     2221 tttcggaata acccttgatc cggtgtatat cggtgccggt ggatatagac tcggtatggt
     2281 agataatacc attgtcagag atcctgccac agatgtgcca aaaataccag ggacttccct
     2341 tgcgggtgca ataagagagt tcgctactgt ctatacaatg acaaatgcct gccaaagtga
     2401 aaatgatgaa aaagagaaga gaaaatatgc tgaagaagaa gtcaaaaaat attttggtga
     2461 cgaaaacaga cagggaatgg tgagattcta cgacggacaa atcatctttt ttcccgtatc
     2521 ttctattcaa ggaacagtct ggttgactac cagggatata cttgaatact ggctgggaga
     2581 aataaaagac ccacaaggag agaaaataaa gattcctgat aaaatcgaag ataatgccta
     2641 tgcaataaag ggaatcgata gctctaaacc actaaatctt ggctggcttt tattggatac
     2701 tcagaatatt aacggaggaa aagaaattac attgccgccg gaacttgata aattcttaaa
     2761 aaggattgtg attgtttcag ataaattatt ttctcatatt gtaaatgaca atttagaagt
     2821 gcgcacatct gtaaggatcg atccagaaac aggaactgct gcagaaggtg cattattcac
     2881 ttatgaagca ataccaagag gaacgattct gggatttgaa atggttacag ataccagaaa
     2941 aggagataat gtagagagca tcgttaattt gatagaagcg acgtttccat acctaaaact
     3001 tcttggacta ggtggaatgg gaacgcgagg ttttggaagg gttaagtttt tcaccttaaa
     3061 aaacaataag cagtcaaata ataaaatgga gggtaacgaa aatgacaaat cttgattata
     3121 tatgtgcaaa aattggtcag aatttagtag taaaagctga cgagaatatt tttagaaaag
     3181 ctcttggtat tttacaagaa gatggtgtat atgccatgtt tctctggctg gaaaatgaag
     3241 atagtgaaat aagaaaaaag ctaaacagtt tactaaatga gaatgaaata aaaagcaaac
     3301 ttctttcaga ttctaaaagt tttcctgatg actttaaag
//
LOCUS       DAOSUD010000141         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_12717_length_3112_cov_26.214917, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000141 DAOSUD010000000
VERSION     DAOSUD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12717_length_3112_cov_26.214917"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..523)
                     /locus_tag="PLG49_10770"
     CDS             complement(59..523)
                     /locus_tag="PLG49_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOQ17826.1"
                     /translation="MLLEDQKSHEEVCIKQYRDYASRAKDPQLKQLFNDYAAKEQEHL
                     NTINQLLNGQMPQMSSSQGQQGNQNSQGMSMGQNNNQSASQGQNYNQADALLCQHMLM
                     TEKYVSGTYDTTIFEFQCPNIRQILNHIQKEEQEHGQGIFDYMYQHGMYTPQ"
     gene            720..1721
                     /locus_tag="PLG49_10775"
     CDS             720..1721
                     /locus_tag="PLG49_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ17827.1"
                     /translation="MNRKKTCILMLLIMVMIGACFSGCSKEDLTVVRLNEVVHSVFYA
                     PQYVAIEKGFFEEEGLKIELTSGWGADKSMAALISGDADIGMMGTEAAIYVYNQGKEN
                     YGVIFAQQTQRAGNFLISRENEKDFQWTDLKGKTVIGGRPGGMPQMVFEYILKQNGLK
                     PFEDVEIITNLQFTATAGAFVSGTGDYTIEFEPTASTLEEQNNGYVVASLGTASGKVP
                     YTVFMASKEYIENNPEIIQKFTNAFYKGQVWVKEHSPEEIAEVIHPQFKETDKELLVK
                     IIRRYKEQDTWCETPIMDPEGLELLQDILESAGELDKRVPYDAIVTTEFAEKAIENN"
     gene            complement(1782..2969)
                     /locus_tag="PLG49_10780"
     CDS             complement(1782..2969)
                     /locus_tag="PLG49_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOQ17828.1"
                     /translation="MGVKITESVTWVGIRDWELRTFHGEEYSTHRGSSYNCYLVRDEK
                     TALIDTVWSPFDKEFVANLKKEIDLNEIDYIIINHGEVDHSGALLQLMNAIPETPIYC
                     TKNAATSLKGQYHKDWNFNVVKTGDKLSLGKKELIFIEARMLHWPDSMFTYLTGENIL
                     FSNDAFGQHYATEEIYNDLADQCALYQEAIKYYANILTPFSALVTEKIKEVLSFNLPL
                     NMICTSHGVIWRDNPAQIIEKYLSWADNYQENQITIIYDTMWDNTKKMAEAIKKGIRA
                     ADSEVVIKLFNAGKTDKNDIITEVFKSKAILVGSSTINNGILSSVAGILEMIKGLAFK
                     NKKAAAFGSYGWGGQAVKIISNLLKESGFELINEGKKVMWTPDENALKECEEFGAQIA
                     KAF"
BASE COUNT          961 a          569 c          503 g         1079 t
ORIGIN      
        1 aatataaata aaaaccatat tacattatgt aatacggttt ttatttaaaa aagtctattt
       61 attgtggcgt atacattcca tgctgataca tataatcaaa aattccttgt ccatgttctt
      121 gctcttcttt ttgaatatga tttaatattt gacggatatt ggggcattga aattcaaaaa
      181 tagtcgtatc atatgtccct gaaacatatt tttctgtcat cagcatatgt tgacatagaa
      241 gtgcatctgc ctgattataa ttttgtccct gactggcaga ctgattattg ttttgtccca
      301 tagacatacc ctgactgttt tgatttcctt gctgtccctg agatgaagac atctgaggca
      361 tttgtccatt aagtagctga ttaatggtat ttaaatgttc ctgctctttt gcagcataat
      421 cattgaatag ctgttttaat tgaggatctt ttgcccgact ggcataatcc ctgtactgtt
      481 taatgcatac ttcttcatgg cttttttgat cttctaaaag cattctttct ttttgtgtta
      541 attgaacttt ttgtgcaaat tgaaccgtca aattaaacgc ctcccgtttt attttttgcc
      601 ctttttatta tttccataaa aagattaaat atgtaagcta aattaaaatc tcctcttcaa
      661 tcacgaatac atccccttga tgaatatata tattatatat tcaaaaaggg ggaaattata
      721 tgaacaggaa gaaaacatgc attcttatgc ttcttatcat ggttatgatc ggtgcgtgtt
      781 tttcaggatg cagcaaagaa gatttgacag ttgtaagact gaatgaagtt gtacattctg
      841 tattctacgc tcctcaatat gtggcaatag aaaaaggatt ttttgaagaa gaaggcctta
      901 aaatcgaact tacatcaggc tggggtgctg ataaatccat ggcagcccta atttcaggcg
      961 acgccgatat aggaatgatg ggaactgaag cagcaattta tgtttacaat cagggaaaag
     1021 aaaactatgg cgttatcttt gctcaacaaa ctcaaagagc aggtaacttt ttaatcagcc
     1081 gtgagaatga aaaagatttc caatggaccg accttaaagg taaaaccgtt attggcggtc
     1141 gtcccggtgg aatgcctcaa atggtctttg agtatatttt aaaacaaaat ggcttaaaac
     1201 cttttgaaga cgttgaaatt attacaaatt tgcaattcac tgcaacggca ggggcctttg
     1261 tcagcggcac aggcgattat accattgaat ttgaaccaac cgcttctact cttgaagaac
     1321 aaaacaacgg ttatgttgtt gcttcattag gaactgccag tggtaaagtt ccttatactg
     1381 tctttatggc atctaaagaa tatattgaaa ataatccaga aatcattcag aaattcacca
     1441 atgcttttta taaaggtcaa gtatgggtta aagaacactc tccggaagaa attgcagaag
     1501 ttatccatcc tcaatttaaa gaaaccgata aagaactttt agttaaaatt attagaagat
     1561 ataaggaaca agatacctgg tgtgaaacac ccatcatgga tccggaagga cttgaactgc
     1621 ttcaggatat tttagaaagc gccggagaat tagataagag agtaccttat gacgctatcg
     1681 tcaccacaga atttgcagaa aaagccatcg aaaataacta aatggtaaat atgatataga
     1741 cagaaaaacc atggttataa ccatggtttt tctctttcat attaaaaagc ttttgcaatc
     1801 tgagcgccaa attcttcaca ttcttttaag gcattttcat ctggtgtcca catcactttc
     1861 tttccttcgt taatcaactc aaaaccgctt tcttttaaaa gattagagat aatttttaca
     1921 gcttgtcctc cccagccata gcttccgaat gctgctgctt ttttgttctt aaaagctaaa
     1981 ccttttatca tttctaaaat gcctgcaacg gaagataaga ttccattgtt aatcgtcgaa
     2041 gagcccacca gaattgcttt tgacttaaat acttctgtga taatatcatt tttatctgtt
     2101 ttgccagcat taaaaagttt gataaccact tcactgtcag cagcccggat tccttttttg
     2161 attgcttcag ccattttttt cgtattgtcc cacatcgtat cataaataat tgtgatttga
     2221 ttttcctgat aattgtctgc ccaacttaag tacttttcta taatctgggc aggattatcc
     2281 cgccatataa cgccatggct tgtacaaatc atattaagag gcaaattaaa actcaggact
     2341 tctttaatct tttctgtcac caaagcacta aaaggtgtca ggatattggc ataatatttt
     2401 atagcttcct ggtaaagtgc gcattgatcc gctaagtcat tataaatttc ttctgtagca
     2461 taatgctgac caaaagcatc attactaaat aaaatatttt ctcctgtaag ataagtgaac
     2521 atactgtcag gccagtgaag cattctggct tcaataaaaa tcaattcttt ttttcctaaa
     2581 cttaatttat cacctgtctt taccacgtta aaattccagt ctttgtgata ttggcctttt
     2641 aaggaagttg ctgcattttt tgtacaatat attggagttt caggtattgc attcatcaat
     2701 tggagcagtg caccagagtg gtcaacttct ccatgattaa taataatata atcaatttca
     2761 tttaaatcta tttctttttt caagtttgct acaaattctt tatcaaatgg actccatacg
     2821 gtatcgatta aagctgtttt ttcatccctt accaggtagc aattatagga agaacccctg
     2881 tgagtcgaat attcttcacc atgaaaggtc cgaagttccc agtctcttat tccaacccag
     2941 gtcacacttt ccgtaatttt aactcccatc tttcatcctc cttgttatta tttttattta
     3001 gaaaaagttt gctgtttata gaaaattttt aagccgcaat ttaaaccctt ttaagctttt
     3061 aacatatatt atttagtatt aattcaattg ttttttctat tcaaattgat at
//
LOCUS       DAOSUD010000142         3089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_12843_length_3089_cov_26.333223, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000142 DAOSUD010000000
VERSION     DAOSUD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12843_length_3089_cov_26.333223"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1701)
                     /locus_tag="PLG49_10785"
     CDS             complement(328..1701)
                     /locus_tag="PLG49_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOQ17829.1"
                     /translation="MASYIKGLDKDKELFISKLSTQAKENNYIDPALYAKYEVKRGLR
                     DISGKGVLAGLTEVGEVHAYVLDENELIPVPGRLIYRGIDIYDLTKGFLEENRFGFEE
                     VAYLLLSGQLPSEEILKEFKAFLEDSQAMDDVFLRNTILEIPSKDIMNTLARGVLTLY
                     NYDNNPDDISIENVIRQSLELIAKLPLIAVYGYQAYVHKYLNQSLVLHSPKAGLSIAE
                     NILHMLRPDSKYSELEAVILDLLLVLHAEHGGGNNSTFTTHVVTSTGTDTYSAIAAAI
                     ASLKGPRHGGANIKVRQMFNVMKEELKDWEDEDEIRHYLLKILRKEAFDRSGLIYGIG
                     HAVYSISDPRSVILKGYAEQLAKEKGYDEEYQLYERVERLAPEVISKERKMYKGVCAN
                     VDYYSGLVYNMLGIPGELFTPLFAVSRVVGWCAHRIEELVNPGKVVRPAFKSVAERKE
                     YIPLKNR"
     gene            complement(1812..2633)
                     /locus_tag="PLG49_10790"
     CDS             complement(1812..2633)
                     /locus_tag="PLG49_10790"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOQ17830.1"
                     /translation="MFFEIIKVIIIGIVEGITEWLPISSTGHMILLDELIKLNASDAF
                     KEMFLVVIQLGAILAVILLYFNKLNPFSVKKTAQEKKETLQIWYKVIIGVIPAAIFGL
                     ILEDWLDEHFYNYITVAIMLIIYGILFIVIENKNKDKKSTINDFNHLSYGTAFSIGLF
                     QVLALIPGTSRSGATILGAILLGTSRYIAAEYSFFLSIPVMFGASALKLIKFGFDFST
                     MEIVLLLTGMSVAFFVSVIAIKFLLGYVKKNDFKAFGWYRIVLGLLVIGYFFLFK"
BASE COUNT         1083 a          576 c          372 g         1058 t
ORIGIN      
        1 aatctttaag tctttcaata cttgtaaaag gcctccttta gataagattt gactcgtaag
       61 ctttaatatt aatccatttt cttattgttg attctatcaa acatatttct atcgcacaat
      121 aaatattaat aacatatttt tgtattatta tatcactttt ttattaagtc attctcttaa
      181 tcataagcta tgaactattc ttaagcattg tactttgctc aaaacaaata ttctgcttaa
      241 aaaaacggca taaccggtaa tatacccagc tatgccgatg tttctttagg aaatcataac
      301 tgctgaaaaa caagctaaat gtgcttatta tctgtttttt aaaggaatat attcttttct
      361 ttctgcaaca ctcttaaatg ccggacggac tactttacca gggtttacta attcttcgat
      421 acggtgagca caccagccta ctactctgga aacagcaaaa agcggtgtaa acaattcccc
      481 tggaataccc aacatattat aaaccagtcc ggaataataa tcgacattag cacaaacacc
      541 tttgtacatt ttacgctctt tagaaattac ttccggagcc aaacgttcta ctctttcgta
      601 taattgatat tcttcatcat aacccttttc ttttgccaat tgctcagcat acccctttag
      661 aattacagat cttgggtcag aaattgaata aactgcatgt ccaataccat aaataagacc
      721 agaacggtca aaagcttctt ttctcaaaat tttaagcaga taatgcctga tttcatcctc
      781 gtcttcccaa tctttcaatt cttctttcat tacattaaac atttgtctta ctttaatatt
      841 ggctccacca tgtcttggtc cttttaagga agcaatggca gctgcaatag cagaatatgt
      901 atctgttcca gttgaagtaa ccacatgggt tgtaaatgta gaattattgc ctcctccatg
      961 ctcagcatga agaaccaata acaagtctaa tataactgct tcgagttctg agtacttaga
     1021 atccggacgc aacatatgca aaatattttc cgcaatactc aaacctgctt tgggactatg
     1081 aagcactaaa ctttgattta aatatttatg aacataggcc tgataaccat aaacagctat
     1141 taaaggtaat tttgcaatta actccaggct ttgtcttata acattttcaa tagaaatatc
     1201 atctggatta ttatcataat tatataaagt taatacacct ctggctaaag tattcattat
     1261 atccttgctt ggtatctcta aaattgtatt tctgaggaaa acatcatcca ttgcctgaga
     1321 atcttcaagg aatgctttaa actcttttaa aatttcttct gaaggcaact gtccgcttaa
     1381 taacaagtat gcaacttctt caaaaccaaa tctgttttct tctaaaaatc cttttgttaa
     1441 atcataaata tctatacccc ggtaaattaa cctgccagga acaggtatta attcattttc
     1501 atcaagaaca taggcatgga cttctcccac ctcagtaaga cctgccaaaa ctccttttcc
     1561 gctgatatca cgaaggcctc ttttcacttc atatttagca tacagtgcag gatcaatata
     1621 attattttct ttggcttgcg tacttaattt actaataaat aattctttat ccttatctaa
     1681 tccttttata taactcgcca tattatcgcc tcctttttta tctgttaaaa ataatcatat
     1741 catgcaagaa attctcaaag caagcatatt tttattaaaa tataaaaaaa cttaacaaaa
     1801 tattgaaata tctatttgaa taagaagaaa tatccaatta ccaatagacc taaaacaatt
     1861 ctataccagc caaaagcttt aaaatcattc tttttaacat atccaagtaa aaacttaatt
     1921 gcaatgacag atacaaaaaa ggctacactc attcctgtta ataataaaac gatttccatc
     1981 gtactaaagt caaatccaaa ttttatcagt tttaatgcgc tggctccaaa catgacagga
     2041 atagataaga aaaaagaata ttctgctgca atatatctgg atgtacccag tagtatcgct
     2101 ccaagaatcg tagcacctga acgggaagtt ccgggaatta aggccaatac ttgaaataaa
     2161 ccaatagaaa atgccgtacc ataacttaaa tgattaaaat cattaattgt agacttctta
     2221 tccttatttt tattttcaat aacaataaaa agaattccat atataattaa cataattgca
     2281 acggttatat aattataaaa atgttcatcc agccaatctt caagaattag accaaatatc
     2341 gcagcaggta taacacctat aattacttta taccaaattt gcaaggtttc tttcttttcc
     2401 tgagctgtct tttttactga aaaaggattt aatttattaa aatataaaag aataacagcc
     2461 aatattgctc ctaattgaat aactactaaa aacatttctt taaacgcatc agaagcattt
     2521 agtttaataa gctcatccag taaaatcata tgaccagtgc tgctaatagg cagccactct
     2581 gtaattcctt cgacaattcc gataataatc acttttatga tttcaaaaaa catttttgac
     2641 cctcctgctt ttaacaatta gattaaaata ccgttattaa cattatgata aaaatcatag
     2701 caccccctag taaataattc aagtatcttt tcaaaatgcg taatccatca tgtttagaaa
     2761 atgtttaaat ttttatcaat aaagtaagca ttattataaa accatcatcc tagtctttta
     2821 tgcttaataa aaattatgtt tgcaaaaaat ttagtattcc atattatctt gtaatttttt
     2881 taagtctctg aaaataaact tgtaatataa tagattccaa tcgttgtaat tagtttgtat
     2941 cttttgaatc ttaagctaat tattgactat ttttttatat cttatataat caaagtagaa
     3001 aaaaaatggg agtgagtaat atgaataaaa taacaaaact attgcctttg atcctgatgg
     3061 caacactttt gttatctgct tgtgaccca
//
LOCUS       DAOSUD010000143         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_12862_length_3086_cov_31.167931, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000143 DAOSUD010000000
VERSION     DAOSUD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12862_length_3086_cov_31.167931"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1403)
                     /locus_tag="PLG49_10795"
     CDS             complement(378..1403)
                     /locus_tag="PLG49_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOQ17831.1"
                     /translation="MPEERINKEHDLGYKHLLTHKKTFIQLLRSFVKEDWVNEIDESQ
                     VMLVDKSYILQDFREKESDIVYRLKIKDRNVIFYCLLELQSSVDYQMPIRLLFYMIEI
                     WRDILKNTENHKIERKGFKLPSIVPIVLYNGVNNWTACRNFKEILDGNEMFSNHVLDF
                     NYILFDVNRYKEEELLGFSNLISGVFLLDQKIDSDQLKDRLVKLIKILKDLSDDEFGI
                     FKGWLKWILKPRISEELRSKIDDILENSKPMEVENMVYNLANTIDEMTQKALEKGIEK
                     GIEKGIEKGIEKGIEKGIEKGIEKGVLKVAKSALEKGADLDFVAQITDLDIEVVKKLK
                     KEIEGTR"
     gene            complement(1614..1727)
                     /locus_tag="PLG49_10800"
                     /pseudo
     CDS             complement(1614..1727)
                     /locus_tag="PLG49_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(2064..>3086)
                     /locus_tag="PLG49_10805"
     CDS             complement(2064..>3086)
                     /locus_tag="PLG49_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ17832.1"
                     /translation="IRVILQNKGSYLVLFFGIFLSSFLLMFGIGLKPLMDYYVDTIDD
                     TLPYEYQYILKGPVEAEGGEKVLIYSLETEYYLTGNNVEVTFYGIQEDSEIFTDFTIP
                     TEENHIVVSDSFAKKLKVDVGDEIIFEDTVYEKKYTLVVDEICPYTNNLGAYMSIEDL
                     CKLLDKDAGTFNCYVSQEKLDIDDAYIAKFMNRSDFLGAAKQMMISFGSVIAIINVFS
                     VFAYLVLMYLLTKIVIDKNSLYISFMKVFGYEKKEIRRLYLDASAIVVLVSLLICLPL
                     EAYLIKMVLIYISSLIEGYMDFYLPASVYLQIVVIGAVAYFAINALHLQKVKRIPMNE
                     ALKNRE"
BASE COUNT          950 a          659 c          362 g         1115 t
ORIGIN      
        1 attttatatt ttatcataga aagcttattt tacagagttt ttttcacagt ctccaaagga
       61 acgttgatac tactatacat aatataatta aaatcattct tatggcaaca ataagctttt
      121 ccttattact cattaatgac ctcaatctct ttcatacttt ttctcataat catacctaaa
      181 actgctctgt aatcataaga aatgtcatat tataacattt ttcaattatt ggtttttcta
      241 ttttccagaa actccaaatg tctttaaaca agtttcacaa tcaaaacaga ttctaataaa
      301 aatacagggg agtaatcccc ccttgttttt gcttaagcat gctggtataa atacttttga
      361 tacactgaaa aaagatatta tcttgtcccc tcgatttctt tctttagctt cttaactact
      421 tctatatcca aatcagtaat ttgggcaaca aaatctaaat ctgccccttt ttccagtgct
      481 gactttgcaa cttttaatac tcccttttca attccttttt caattccctt ttctattcct
      541 ttttcaattc ccttttctat tcccttttct attccctttt ctattccctt ttctagagct
      601 ttctgagtca tttcgtctat tgtattagca agattgtaaa ccatattttc cacctccata
      661 ggtttcgaat tttccagtat atcatcaatt ttcgatcgca attcttccga tattctcggc
      721 tttaaaatcc atttaagcca acctttaaat ataccaaact catcatccga aaggtccttc
      781 aatatcttta taagttttac caatctgtct ttcagttgat ccgaatctat tttctgatcc
      841 agtaaaaaaa ctccggatat aagatttgaa aatcctagta gctcctcttc cttatacctg
      901 ttcacatcaa acaatatata attaaaatcc agtacatgat ttgaaaacat ttcattgcca
      961 tcaagtattt ctttaaaatt tctgcatgct gtccaattgt ttacaccatt atatagtact
     1021 ataggtacta tagatggaag tttgaatcct ttccgctcta tcttatgatt ctctgtgttt
     1081 tttagtatat cacgccatat ctctatcata tagaacagca atcttatcgg catttgataa
     1141 tctactgacg attgcaattc caacaaacaa taaaatatta cattcctgtc ctttattttt
     1201 agcctataaa ctatatctga ttctttttct ctaaaatctt gtagaatata acttttgtct
     1261 accagcatga cctggctttc atctatttcg ttgacccaat cctctttaac aaaacttctt
     1321 aatagttgga taaaggtctt tttatgcgtt aataaatgct tgtatcctaa gtcatgctcc
     1381 ttatttatcc tttcttccgg cattctttta cctcttttta ttccttttat ctatagtata
     1441 ccatttttca tctactgtga aaatatttat taaaaagctt ggcttcaatc attccttatg
     1501 cttttcatat agtcttatga gttctatatc ccgataccta tctttgattt ttcaaataga
     1561 tagattatta atttataatc cagtcggcaa taaattcacc tgtatcagtt ttataaagcc
     1621 atttctacct cttcttcctc ttttgctcct ataacaacca gcgcaataaa atatccttcc
     1681 attggatata atgtacataa tgatttaccg ctattttgat attttaccat tcacaacacc
     1741 ttttcattat agatcaatcc atactttacc atttccaact ctgatatggg atacaatgca
     1801 aaatagaagc tttaaaagaa taatttttca ttatttcgta ttttatctaa ttcaataatt
     1861 ttcgtccaca ttattttact gataaaagct cgggacatca agtcccttcg cttttgcctc
     1921 tttcatttca ttccaaaaga ccaaataaaa actcgggaca tcgagtccct tcgctttttg
     1981 gtcatttcac ttcgtgaaat cccactgata attacgagac ttcaagaagt ctcgtaatta
     2041 tgcaatgtat atttcgaata gctttattcc ctgttcttca acgcctcatt catcggtatt
     2101 ctcttcactt tctgaagatg taaagcatta atcgcaaaat aggccactgc tccaattacg
     2161 acaatctgca aataaaccga agccggcaga taaaaatcca tatatccttc aattaatgaa
     2221 gatatgtata taagaaccat tttaatcaaa tatgcttcga gcggaaggca aatcagcaag
     2281 gaaaccagaa ccacaattgc tgaagcatcc aaatacagtc tcctgatttc ttttttctca
     2341 tatccgaaaa ccttcataaa agaaatataa agagagttct tgtcaatgac aattttggtc
     2401 agaaggtaca tcaataccaa ataggcaaat accgagaaca catttataat ggcaataaca
     2461 cttccaaagg aaatcatcat ctgctttgcc gccccaagaa aatcgcttct gttcatgaat
     2521 tttgcaatat aagcatcgtc aatatccagt ttttcctggg atacatagca gttaaaggtg
     2581 ccagcatcct tgtcgagaag cttacacaaa tcttcaatgc tcatatatgc ccccagattg
     2641 ttggtatatg ggcagatttc atccaccacc aaagtgtatt ttttctcgta aacggtatcc
     2701 tcaaatatga tttcatcccc tacatcaact tttagctttt ttgcaaagga atcggatact
     2761 acaatgtgat tttcctccgt cggtattgta aaatcagtaa aaatttcact gtcttcctgt
     2821 attccgtaaa aggtgacttc cacattattt cctgtaagat aatactcagt ctctaaagaa
     2881 taaattaata ccttttctcc cccttccgct tcaaccgggc cctttaatat atactgatat
     2941 tcatagggca gagtatcatc aatcgtatcc acataataat ccataagagg ctttaagcct
     3001 atgccaaaca tcagcaggaa gctggataaa aagatcccga aaaacagaac caaataactt
     3061 cctttgtttt gaaggataac acgaat
//
LOCUS       DAOSUD010000144         2989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_13387_length_2989_cov_30.970007, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000144 DAOSUD010000000
VERSION     DAOSUD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13387_length_2989_cov_30.970007"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..716
                     /locus_tag="PLG49_10810"
     CDS             135..716
                     /locus_tag="PLG49_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17833.1"
                     /translation="MRDFKGLCKWIDDTSKIPESVQNFRAQLRSSAYTFKDVKKPNSS
                     KYIGVFNLLFRNEAKDFYRNAPISFNELEDHHIFPKNFVRRHNADVEWDTVLNRTLIL
                     DKTNREISNKSPAEFIMQSIKNRVEAGKISEYQAEADLKESLKAHFIDEEMYLILKGT
                     DDSLTTEQIEVNFTRFIEKREQLINERIQSLIN"
     gene            1070..2773
                     /locus_tag="PLG49_10815"
     CDS             1070..2773
                     /locus_tag="PLG49_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ17834.1"
                     /translation="MKISGVRIHNFRSIKDQSFTLKDFSLLVGANNCGKSNIIDAIRL
                     FYGKNVKFVTENDFPKFDTTDKNSWVEMEFKLTDHEYKTLDDNYKQPGNKLKLRRCFF
                     ENGEVKSDSQSNIYGYKKDELSKDLFYGWKNVAQAKLGNIIYVPATSEVNDYTKLTGP
                     SPFRDLLDFVMTNIETSDAFQSFLSSCNQFMENLHSGQLPQGFSVMDLENLITSELED
                     WQFRFNIGISELKPKDIIKMMFCPSLTDEYLENKPVDMNYVGQGLQRHLIYALIKASI
                     TLPKPQEDPIKNHQFSPDLTLILFEEPEAFLHPLQQETLNRNLCQLAQEENQQVLIST
                     HSPHFVSKNINKLESIIKLNKQSGETLLKQIDEPVMNEIIEGNTDFKKIVDKRNTNGS
                     NQNSAIDEDDEEREKILYMLYLDPDRACSFFADNVLICEGITEKAFIDYLFEQGKISF
                     KEKANIINAGGKYQIPRFMDLFQALGIEFAVLFDGDNDKKNHEAINNYIEEQCHKNGV
                     PVHKFSKDLEDFLGVERVSNKKDYLKPLNLLWKYEHNCIDEKKVRDFISLVESLVALP
                     K"
BASE COUNT         1047 a          535 c          575 g          832 t
ORIGIN      
        1 acaacaccat acggggtgct gcaggctgtg ccgtactaaa tgccgagatg tacgaggcga
       61 cgaacaaatt acacccataa ttttggagtt ctgcattttc tgaacgttat tcaagttcta
      121 cagaaacctt gattatgaga gatttcaaag gcctatgcaa atggatcgac gacacgtcaa
      181 agattcctga gtctgttcaa aactttagag cacagctacg gtcatctgca tacacattta
      241 aagatgttaa aaaacctaat agctcaaagt atatcggtgt gtttaatctg ctatttagaa
      301 acgaagccaa ggatttctac agaaatgcgc ccatatcatt taacgaactc gaagatcacc
      361 acatattccc gaaaaatttt gtacggcggc acaatgcaga tgtggagtgg gacaccgtat
      421 taaacaggac cttgattcta gataagacaa acagagagat ttccaataag agccctgcag
      481 aatttataat gcaaagcata aagaaccgtg tagaagcagg gaaaatctca gaataccaag
      541 cagaagctga cttaaaagag tccctaaaag cacactttat tgatgaggaa atgtacctta
      601 tccttaaagg aacagatgac tcccttacca ccgaacaaat agaagtaaat ttcacgcgtt
      661 tcatagaaaa aagagaacaa ctcataaacg agagaattca gtcgttgata aattaatgga
      721 aacatggtct tacctatttc tttcttgaat agatctgcta attaagatct gaggaaaaca
      781 tcaactccaa gaagacatta gaggtattgg cattacaaga ataggcacta ctatactggt
      841 attatcaaac cagtaacctc tgtccacatg cgatattttt gggcacaggt atggaagaac
      901 acttttttca gtgtatattt ctggcagctg gcagcgctta gacatccaga ttagtgggaa
      961 acttttcaat atccaatgta agtactattg cattgcaact taaaaggaat atggtagcct
     1021 gaataaaggt tcttcatttt tgagcataaa atgccaggga ggtctaatca tgaaaataag
     1081 cggggtcaga atccataatt ttcgatcgat aaaagatcaa tcgtttacct taaaagattt
     1141 ttcactttta gtaggtgcga ataattgcgg taaatctaat attattgatg caataaggct
     1201 cttttacgga aaaaatgtta agttcgtcac tgaaaatgac tttcccaaat tcgacaccac
     1261 cgataaaaac agttgggtcg agatggaatt taagctaact gatcacgaat acaaaaccct
     1321 tgatgacaat tataaacaac ctggaaacaa attaaagttg cgcagatgct tctttgaaaa
     1381 tggcgaagtt aaatctgata gccagagcaa catatacgga tataaaaaag acgaattatc
     1441 taaagacctt ttctatggct ggaagaacgt cgcacaagca aaactaggga atatcatata
     1501 cgttccagca acaagcgaag tcaatgacta cacaaagctg acagggcctt ctccatttcg
     1561 cgatttgctt gatttcgtaa tgacaaatat tgaaacaagc gatgctttcc aatcattttt
     1621 aagctcctgc aatcaattca tggaaaattt gcacagcgga cagttacctc aaggtttttc
     1681 agtaatggat ttggaaaatc ttatcacttc ggaactagaa gattggcaat ttagatttaa
     1741 tatcggtatc tctgagctta aacctaagga catcataaag atgatgtttt gtccttcctt
     1801 aacagatgaa tatcttgaaa acaaaccagt tgatatgaat tacgtggggc agggcttgca
     1861 aaggcatcta atttatgccc ttatcaaggc gtcaataacc ctacccaaac cacaagaaga
     1921 cccaattaag aatcaccaat tttcaccaga tttgacgcta atcttatttg aagaaccaga
     1981 agcatttctt catccactgc agcaggaaac gctcaacagg aacttatgtc agcttgcaca
     2041 agaggagaat cagcaagtac taatttccac gcattcacca cattttgtta gcaaaaacat
     2101 caacaaacta gaaagcatta taaagttgaa caaacaatca ggagaaactt tgttaaagca
     2161 aatagatgag ccggtaatga acgagatcat agagggcaat actgacttta agaagattgt
     2221 tgataagcgt aatacgaatg gatcaaatca aaacagtgct attgatgaag acgacgaaga
     2281 aagagagaaa attttgtata tgctctattt agaccctgat agagcttgct ccttcttcgc
     2341 agataatgta ctgatctgtg aaggaataac tgaaaaagcg tttatagatt acctttttga
     2401 acaaggcaag atctccttta aagaaaaggc caacataatc aatgctggag gtaagtatca
     2461 gataccccgg ttcatggatc tttttcaggc tttgggcata gagttcgcag ttttgttcga
     2521 cggagacaat gataaaaaga atcacgaagc gattaacaat tacattgaag aacaatgcca
     2581 taaaaatgga gttcctgtac acaagttttc taaagatttg gaagattttc ttggagtgga
     2641 gcgtgtatca aataagaaag actacctgaa gcccttaaat ctgctgtgga aatatgagca
     2701 taattgtatt gacgagaaga aggtaagaga ttttattagc ctagttgaga gtttagttgc
     2761 tctgccaaaa tagtattcat agtattgttt atagatctac agccgtttat acgtgtaaca
     2821 ttttgctatt ttttgtcatt atcatacacg catgaaacct cgttgaatag gaatcgtctt
     2881 tgagtaaagc aaaaagttct tcttaaataa cctttttata tctttttgcc cttctgagtt
     2941 gttttattag ttagatgcgg ctaaagtggt gttatggtgg acgaaataa
//
LOCUS       DAOSUD010000145         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_13507_length_2967_cov_16.046360, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000145 DAOSUD010000000
VERSION     DAOSUD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13507_length_2967_cov_16.046360"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1611
                     /gene="aldA"
                     /locus_tag="PLG49_10820"
     CDS             163..1611
                     /gene="aldA"
                     /locus_tag="PLG49_10820"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOQ17835.1"
                     /translation="MKRYKLFINGEFVESTSNRMIKVINPANEEVISEVPEGSVEDTR
                     KAIDAAFDSQKKWEKLPAIERAKYLRKIADGIRKNSKMLTETLMEEQGKIRSLAETEI
                     NFTADYIDYMSEWARRIEGEIIPSDKPNENILLYKAPIGVVAGILPWNFPFFLIARKM
                     APALITGNTIVIKPSSETPNNAFEFAKIVAESGLPKGVFNLVTGRGSVVGEELASNPK
                     VGMVSFTGSVEAGSRIMQLAAKNITKVSLELGGKAPAIVMDDANIDDAVKYIKQSRVI
                     NTGQVCNCAERVYVHEKVADEFIEKMVKAMQETKYGEPLKEDDIEMGPLISKSALESV
                     KGFVERAIKQGAKLLTGGEDDKFKKGFYYKATVLANCTQDMEIIQKEIFGPVLPIVTF
                     KDLDQAIEYANDCEYGLTSSIYTQNLDVMMRAANEIKFGETYVNRENFEAMQGFHAGW
                     RKSGIGGDDGKHGVEEFLNTHVIYIQYDTKKK"
     gene            1633..2928
                     /gene="larA"
                     /locus_tag="PLG49_10825"
     CDS             1633..2928
                     /gene="larA"
                     /locus_tag="PLG49_10825"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOQ17836.1"
                     /translation="MKVEIPYGRETITLSLSDKLNTVLLKKSEVGGLRNVKTRIKKAL
                     LNPIDSPSLLNLAKGKQDCCIVISDVTRPVPNSLLLPLLIEELKASGIKKENICILIA
                     NGSHDPVPEEMYEELIGKEVLKENIEIINHNAYDDNQLKELGETSSGCPVKVNKRYFE
                     SELKICTGLIEPHFMAGYSGGRKSICPGIVGIETLKVFHGVQTMGDPRSKSCQLENNP
                     VDKIAQEVALMAGCDFILNVTLNEKKEITGIFAGDIFKAHQLGCNFVANNSIVEIDSP
                     VDIVITSNGGYPLDQNFYQTVKGLVEAAEILKPSGVIIMASKCERGIGKSEFIELLLE
                     LKEKGIEEFLKGHETSETFKSDQWEVQKLTQVLEKTNNIFMMSSLKDEEYKYTFTSKI
                     NYIEEGLEKAIKLKGINAKIAIIPEGPYVLGKIKSAKER"
BASE COUNT         1163 a          382 c          599 g          823 t
ORIGIN      
        1 tatcttctga actagaggct ttagttaaga gatttaaaat ataattatta aaaaggctgt
       61 gaaataaaaa atttgataaa gaagattcta taatatttgt caaatacgag ttttttgttt
      121 taaaggaacc taaaaactat ttttgatggg gaggtataag gtatgaaaag atataaactg
      181 tttattaatg gtgaatttgt ggaatcaacc tcaaacagaa tgataaaagt tataaaccca
      241 gccaacgaag aagtaatttc tgaagtacct gaaggaagcg tcgaagacac aagaaaagca
      301 atcgatgcag cctttgattc acaaaaaaag tgggaaaagt tgccagcaat agaaagggca
      361 aaatatctaa gaaaaatagc agatggaata agaaaaaatt ctaaaatgtt gacagagact
      421 ctaatggaag agcaaggtaa gattagaagc ctagcagaaa cagagattaa ttttactgca
      481 gattatattg attacatgtc agaatgggct agaagaatag aaggagagat cattccgagc
      541 gataaaccta acgaaaatat attgttatac aaagctccaa taggtgtagt tgctggcata
      601 ttaccttgga attttccttt ctttttaatc gcaagaaaaa tggctcctgc tttaataaca
      661 gggaatacta tagttataaa accaagttct gaaactccaa acaatgcttt tgagtttgca
      721 aaaatagtgg cagaaagtgg ccttccaaaa ggtgtgttca acttagtaac aggcagagga
      781 tcagttgtag gtgaagagct agcttcaaat ccgaaagttg gaatggtaag ttttacaggt
      841 agtgtagaag caggctcgcg aataatgcaa ttagctgcaa aaaatattac aaaagtttca
      901 ctagaattag gtggaaaagc tccagcgatt gttatggatg atgccaatat agacgatgct
      961 gtgaaatata taaagcaatc aagagttata aatacaggcc aagtttgtaa ctgtgctgaa
     1021 agagtttatg tacatgaaaa agtcgctgat gaatttatag aaaagatggt aaaagcaatg
     1081 caagaaacaa agtatggaga acctttaaaa gaagatgaca tagagatggg acctttaatc
     1141 agcaaaagtg ctcttgagtc agttaaaggt tttgtagagc gagcgataaa acaaggggca
     1201 aaattactaa caggcggaga agatgataag tttaaaaaag gattctatta caaagcaaca
     1261 gttctagcta attgtaccca agacatggaa ataatacaga aagaaatctt tggccctgtt
     1321 cttcctatag tcacttttaa agatcttgat caggctattg aatatgcaaa cgattgtgaa
     1381 tatggtttaa cttcatctat ttatactcaa aatcttgatg ttatgatgag agcagctaat
     1441 gaaataaagt ttggagaaac ctatgtaaat agagaaaatt ttgaagccat gcaaggtttt
     1501 catgcaggtt ggagaaaatc aggaattggt ggggatgatg gcaagcatgg tgtagaggaa
     1561 tttttaaata cccatgttat atatatccag tatgatacaa aaaagaaata aaatttaagg
     1621 agaactataa aattgaaagt cgaaattccg tatggaaggg aaacgataac tctatcctta
     1681 agtgataaat taaatacagt gttattaaaa aagtcagagg tggggggatt aagaaatgtc
     1741 aaaactcgta taaaaaaagc acttttaaat cccattgaca gcccttcact tctcaatcta
     1801 gcaaaaggaa aacaagattg ttgtattgtc atatcagatg tcacacgacc tgttcctaat
     1861 tctttacttc tgccgctatt aattgaagaa ttaaaagcca gtggcataaa aaaagagaac
     1921 atatgcatat taattgctaa tggatcacat gatcctgttc cagaagaaat gtatgaagaa
     1981 ttgataggta aagaagtatt aaaagaaaac atagaaataa taaaccataa cgcttatgat
     2041 gacaaccaat taaaagaact tggagaaact tcaagtggat gtcctgtaaa agttaataaa
     2101 cgttattttg agtctgaatt aaaaatttgt actggtctaa ttgagccgca tttcatggca
     2161 ggatattctg gaggtagaaa atctatatgc cctggaatcg ttggaataga gacactaaaa
     2221 gtttttcacg gtgtacaaac aatgggagac ccacgatcaa aaagttgtca acttgaaaac
     2281 aatccagttg ataaaattgc acaggaagta gctttaatgg caggatgtga tttcatttta
     2341 aatgtaactt taaatgaaaa aaaagaaata actggaatat ttgcaggaga catatttaaa
     2401 gctcaccagc taggatgtaa ttttgtagca aataattcga ttgtagaaat agattctcct
     2461 gtcgatattg ttattacctc aaatggtggg taccctcttg atcaaaattt ttatcaaaca
     2521 gtaaaagggt tagttgaggc tgctgaaatt ttaaagccat ctggtgtaat aattatggcc
     2581 tcaaaatgtg aaagaggaat tggaaaaagt gaatttattg aattactttt agaactgaaa
     2641 gaaaaaggga ttgaagagtt tttgaaaggt catgagactt ccgaaacatt taaaagcgat
     2701 caatgggagg tacaaaaatt aacccaagta ttagaaaaga ctaataatat ttttatgatg
     2761 tcatcgttaa aagatgaaga atataaatac acgtttactt ctaaaattaa ttatatagaa
     2821 gaaggcttag aaaaggccat taaactaaaa gggattaacg ctaagattgc tataattcca
     2881 gaaggtccat atgttttggg taagataaaa agtgcaaagg agcgttaatg aatgaacata
     2941 gtggtattag taaaacaagt gccatca
//
LOCUS       DAOSUD010000146         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_13988_length_2883_cov_23.505304, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000146 DAOSUD010000000
VERSION     DAOSUD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13988_length_2883_cov_23.505304"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..804)
                     /locus_tag="PLG49_10830"
     CDS             complement(178..804)
                     /locus_tag="PLG49_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOQ17837.1"
                     /translation="MAVSFDERLRSKLGEEVKIYNIWDDFLAINPNEVGEFTIDNRNR
                     LFFDIKNAELAGVDMIVTTCSTLTPVVEMIRPFIKVPVVAIDDEMARLAVTFGERVLI
                     LATAESTIGPTSSKIAKEAEKAKVKIDLSTKVCKEAFLAMKTMDMEKHDKLLLEMAKD
                     FKAYDCIVLAQASMAHLEKAIHEICACPVLSSPDLCIKQVSELLKMIK"
     gene            complement(854..1267)
                     /locus_tag="PLG49_10835"
     CDS             complement(854..1267)
                     /locus_tag="PLG49_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ17838.1"
                     /translation="MYYQYKKENTIAVPEPFKRYITPLFMADDPVIKETNFSVHITEW
                     EPGCKIDLHAHPDAMEAMYCMSGEGRVKIDGKEYPFTPGSMIVAPPTITHEIENTGKE
                     MLRVFCVFSPPVTGEDLRKRAYAAVEEAKNQSQKE"
     gene            complement(1298..2668)
                     /locus_tag="PLG49_10840"
     CDS             complement(1298..2668)
                     /locus_tag="PLG49_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ17839.1"
                     /translation="MAAKNDFTQGSIPGNILSIAIPMTLAQLISVLYNIVDRIYIGRI
                     PENATLSFTGVGITLPIVTIIIAFANLFGMGGAPLCSIARGSGDNEKAEEIMGNSFAL
                     LLISGVLLTISGFLFKKPLLYLFGASDATFPFANAYITIYLLGTIFVMIGLGMNNFIN
                     SQGFAKKGMLTVLIGAILNIILDPIFIFVLNMGVKGAAIATVISQFVSACWVFKFLTG
                     KETILKLKVKNLRLRKNLVSKITFLGLSGFVMAITNSATQIMCNVCLRIYGGDLYVGV
                     MTVLNSIREVITLPAKGLTDGAQPVISYNYGAGDYKRVKSAIKFMSVLCISYMVISWL
                     ILHSFPAFFIKIFNKDTELVSAGLLPLRLFFFGYFMMSLQFSGQSVFVALGKSKQAVF
                     FSLFRKVIIVIPLTIVLPKFFNLGVNGVFLAEPISNFIGGIASFSTMLFIISREFKSS
                     RSLELN"
BASE COUNT          940 a          562 c          471 g          910 t
ORIGIN      
        1 ttatatatat tatatcattt cctcgactat cattctagta aagtctttag aaaactaaaa
       61 atttggcatt ttattgtaat tataaacaga aaagtctgat caaaaatata aaaaaagccc
      121 ttatcttcat ccgtgctttt gataattata cggattttac gataagagca attgtactta
      181 ttttatcatt tttaatagtt cactgacttg tttaatacat aaatccgggc tggataaaac
      241 cgggcaggca caaatttcat gaatggcttt ctccaaatgg gccatagaag cttgagccaa
      301 tacaatacag tcataagctt tgaaatcttt tgccatttcc agcagtagtt tgtcgtgttt
      361 ttccatatcc atagttttca tggctaaaaa agcttctttg cagacttttg ttgataaatc
      421 aatttttact tttgcctttt cggcttcttt agcaatttta gaactcgtag gacctatggt
      481 actttctgct gtagcgagaa tgagaactct ttctccgaaa gttacagcta atcttgccat
      541 ttcatcgtca atggccacaa caggcacttt aataaaggga cgaatcattt ccacaacagg
      601 agttaaggtt gagcaagtgg tgacaatcat atccactccg gctaactcag cattttttat
      661 atcaaaaaat aaacggtttc tgttatcaat agtaaattcc ccaacttcat ttgggttaat
      721 tgctaaaaaa tcatcccaaa tattatatat ctttacttct tcaccgagtt tagagcgaag
      781 cctctcatca aaactgactg ccacactttt taccgtatgg attaatgcta tactcttaat
      841 aagattacct cctttactcc ttttgtgatt gatttttagc ttcttctact gctgcatagg
      901 cacgttttct taggtcttct cctgtaacag ggggagagaa tacacaaaat acgcgtaaca
      961 tttctttacc tgtgttttca atttcatgtg ttattgttgg aggggcaaca atcattgagc
     1021 ctggggtaaa tggatattct tttccgtcaa tttttactct gccttcccca gacatgcaat
     1081 acatagcttc catggcatcg ggatgggcat gcaaatcgat tttacaacct ggttcccatt
     1141 ccgtaatgtg tacggaaaaa ttcgtttctt ttataaccgg atcatcggcc ataaaaagag
     1201 gggtaatata gcgcttaaaa ggttctggta cagcaatggt attttccttt ttatattgat
     1261 aatacataag gcttcactct cctgtttatc cgtcatttta gtttagctcc aaagacctgg
     1321 aactcttaaa ttcccgtgaa ataataaata acatggttga aaaactcgct attcctccaa
     1381 taaaatttga aatgggttca gccaaaaaga cgccattgac gccgagatta aagaatttgg
     1441 gcaaaacgat ggtcaggggg atgacaataa tcacttttct gaagagagag aaaaatactg
     1501 cctgtttgga tttgcccaaa gccacaaaga ccgattggcc tgaaaattgt aaagacatca
     1561 taaagtaacc aaaaaagaat aatcttaaag gcaggaggcc ggcactcact aattccgtat
     1621 ccttattgaa aatctttata aaaaatgcag ggaaagaatg caggattaac catgaaataa
     1681 ccatataaga aatacagagt acagacataa acttaatagc ggatttgact cttttataat
     1741 ccccggcccc ataattgtag ctaatcaccg gctgggcacc atcggttaat ccttttgctg
     1801 gtaaagtaat gacttcccgg atagaattca ggaccgtcat gacccctaca taaaggtcac
     1861 cgccataaat cctaagacaa acgttacaca taatctgggt cgcactgttg gtaatggcca
     1921 taacaaaacc agacaagccc aggaaagtaa ttttagatac taagttcttt cttaacctta
     1981 aatttttaac ttttagtttt aatatggttt cctttccggt caaaaattta aagacccaac
     2041 aggctgatac aaattgagaa ataaccgttg ctatagcagc gccttttacg cccatattca
     2101 atacaaaaat aaatatagga tccagtataa tgttcaatat tgcaccaatc aatacggtta
     2161 gcattccttt tttagcaaaa ccctgggaat ttataaaatt attcattcca agacctatca
     2221 taacaaaaat tgtacctaaa agatatatcg taatataagc attggcaaaa ggaaaagtgg
     2281 catcactggc gccaaataaa tataataagg gtttcttaaa taaaaaacct gaaatggtca
     2341 gtaaaacacc tgatattaag agcaaagcaa aggagttacc catgatttct tcagcttttt
     2401 cattatcacc actacccctg gcaatggaac ataggggggc acctcccatg ccaaacaaat
     2461 tggcaaaagc aatgataatg gtgacaattg gcaaagttat gcctactcca gtaaaagaga
     2521 gggtagcatt ttctggaatc ctgccaatat aaattctgtc aacgatatta taaaggacgc
     2581 taattaattg tgccaaggtc attggaattg caatgcttaa tatatttcct ggaatactgc
     2641 cttgtgtaaa atcatttttt gctgccatat ctttctgctt ccttcaatga tattttaaaa
     2701 atatttctaa gctgctataa atataaaatt tacagcagct taagttaatc atttatacca
     2761 attcccaagc taagttcaat gtacgctttg catttgctat aacaatttcg ctcttttcat
     2821 ttccgaaggg ctttacttca aaacttacaa tccggcgttc tttatcattt aaataaccta
     2881 ttt
//
LOCUS       DAOSUD010000147         2839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_14277_length_2839_cov_26.897629, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000147 DAOSUD010000000
VERSION     DAOSUD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14277_length_2839_cov_26.897629"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1079)
                     /locus_tag="PLG49_10845"
     CDS             complement(12..1079)
                     /locus_tag="PLG49_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17840.1"
                     /translation="MKKIKASTFDIAGVYIGTVIGAGFASGQEIMKFFTSYGYKGIYG
                     ILLSGVLFSILGWGILEMGYVYKIRDYRGFIYFMMGDFLGNIMEWIVSLFMFISFCTM
                     LAGAGALFKQQFQWPAIVGILIMAALCFLVFLYDIKGIIVINSILVPFLFIGGIYLGI
                     HSIIYKDTAVFSSILMGSFHFFTNNWLSSSLVYVSYNILTAAVILSSLFPAIKSVKEA
                     KRGGILGGAALTLLGLSVVIATLIYYDKIEGLEIPILGIVVNYGQHIEYIFLLVLIAA
                     IFTTAVANGYGFLTRFCDEFDLTYKSVIPYFMLTSVLVALIGFSTMVTKLYSFFGYIG
                     IFELFIILIYFIHYKINKKRI"
     gene            complement(1138..2577)
                     /gene="glgA"
                     /locus_tag="PLG49_10850"
     CDS             complement(1138..2577)
                     /gene="glgA"
                     /locus_tag="PLG49_10850"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOQ17841.1"
                     /translation="MRENQLKVLFVSSEVSPFVKTGGLGDVAGALPYALKKSGIDIRV
                     VMPKYKSIPAEFTKDFKSKADFPVFLDWRKHKASIYEYNKDLPVYFIGNDYYFDREDL
                     YSYEDDHERFAFFCKAVLKMLIRIDFIPDIIHCNDWQTGPIPLLLKENYHDDSFYNSI
                     LTLFTIHNLQYQGIFGKESLRILGVPDWYFNPEHIEFYGNINYMKAGLQYSDAISTVS
                     KTYAREIMTKEYGYGLEGILQKRSQKLYGIVNGIDYKLYNPESDDSLYVKYSPDKIEL
                     KEENKKALQKELNLPQKDVPLISIISRLVEQKGFDLIAEQMDELMKEDIQMIILGTGE
                     ERYERLFSHMEERFPEKVRAKIYFDLDLAQKIYGGSDLFLMPSLFEPCGLGQLISLRY
                     GTIPVVRHTGGLADTIEEFNKETASGNGFVFNEYNSYAMLAAIRRAIAAYHDKESWKK
                     LIRNAMASNHSWEDSAKEYLNLYKELASK"
BASE COUNT         1028 a          552 c          363 g          896 t
ORIGIN      
        1 acatttccgt tttatatccg ttttttattt attttataat gaataaaata aataagaata
       61 atgaaaagct caaaaatccc aatatatcca aaaaaggaat aaagcttcgt gaccatggtt
      121 gaaaaaccaa taagagcaac cagaactgac gttaacataa agtatggaat gaccgattta
      181 taggttaaat caaattcatc acaaaacctt gttaaaaatc catacccatt agccactgcg
      241 gttgtaaaga tggcagcaat cagtaccagt aaaaatatat actctatatg ctgaccataa
      301 ttcacgacta tcccaagaat aggaatctcc aaaccttcta tcttgtcata ataaattaaa
      361 gtggcaatca caacacttaa tcctaataag gtcagagccg ctcctcctaa aatacctccc
      421 cgttttgctt cttttacaga tttaatcgca ggaaaaagac tcgatagtat aaccgcagcc
      481 gttagtatat tgtaagatac ataaactaaa gaagaactaa gccaattatt cgtaaaaaaa
      541 tgaaaagagc ccattaatat ggatgaaaaa acagccgtgt ccttataaat gatactatgt
      601 attcctaaat agatacctcc aataaataag aaaggcacta aaatgctatt aattactata
      661 atacctttta tgtcatataa aaatacaaga aaacacaaag ctgccatgat taaaattcct
      721 acaatggctg gccattgaaa ttgctgctta aacagggccc ctgcccctgc cagcatagta
      781 caaaaactta taaacataaa taaagaaacg atccattcca taatattgcc taaaaaatcc
      841 cccatcataa aataaatgaa gccccggtaa tccctaattt tatagacata gcccatctct
      901 aaaatacccc agcccaatat ggaaaataaa acgcccgaaa gcaaaatacc ataaatcccc
      961 ttatatccat aggaagtaaa aaatttcata atttcctgac ctgaggcaaa gcctgcacca
     1021 ataacagttc caatatatac acccgctata tcaaaagttg aagcttttat ttttttcata
     1081 aaaaaatccc ccatatattt gtttgtaaaa atatatgaaa gattttattt tttcattcta
     1141 tttagacgcc agttctttat aaagatttaa atattctttc gctgaatctt cccatgaatg
     1201 atttgaagcc atggcattac gaattaattt cttccagctt tctttatcat gataagctgc
     1261 tatagctctt cttatagcag caagcatggc ataagaattg tattcattaa agacaaagcc
     1321 atttccactg gcagtttctt tattaaactc ttcgatggta tctgccaaac ctccagtatg
     1381 ccttacaaca gggatagttc catatcttaa gctaatcagt tgcccaaggc cacaaggttc
     1441 gaataaagat ggcatcaaaa acaaatcact gccaccgtag attttttgcg ccaggtccaa
     1501 atcaaaataa attttagctc gtactttctc agggaatctt tcttccatat gactaaacag
     1561 gcgctcatac ctttcttcac cagtgcccag aataatcatt tggatatctt ctttcatcag
     1621 ttcatccatc tgctctgcaa ttaaatcaaa acctttttgc tcaaccagtc ttgaaataat
     1681 actaataaga ggtacatcct tttgcggcag attcaattct ttttgcagag cctttttatt
     1741 ttcttccttt aattcaattt tatcaggaga atatttaaca taaaggctgt catcactttc
     1801 aggattatat agcttataat caatcccatt gacaatgcca taaagctttt gacttcgctt
     1861 ttgaaggata ccttctaaac catagccata ttcttttgtc ataatttctc ttgcataagt
     1921 cttactaact gtactgatgg catctgaata ttgcaagcca gccttcatat aattgatatt
     1981 tccataaaac tctatatgtt caggattaaa ataccaatct ggtaccccta aaattcttaa
     2041 actttctttt ccaaatatcc cttgatactg caaattatga atggtaaaca aggttaaaat
     2101 tgaattataa aaagaatcat catgataatt ttcttttaat aacaggggaa ttggaccggt
     2161 ctgccaatcg ttacaatgaa taatatctgg aataaaatct attcttatga gcatttttaa
     2221 aactgccttg caaaaaaagg caaagcgctc atgatcatct tcataactat ataagtcttc
     2281 tctgtcaaaa taataatcat tcccaataaa atagactgga aggtccttat tgtattcata
     2341 aatggatgcc ttatgctttc tccaatctaa aaaaaccgga aaatctgctt tgcttttaaa
     2401 gtccttagta aattcagcag gaatagattt atattttggc attactaccc taatatctat
     2461 acctgatttt tttaatgcat aaggaagtgc tcctgctaca tcaccaagtc ccccggtttt
     2521 aacaaaaggt gaaacctctg aagatacaaa taaaaccttt agttgatttt cacgcattaa
     2581 tatcccccta cttttcaatg attctacaat agtccatttt tagtctatta tattttataa
     2641 atttaatatt gaagaacttg tctataaatt ctgcactatt caagtctagc attgcggtct
     2701 tttaatgtca atgaagaaaa agagctaaaa aatcacaaaa attgatattg gttaaaaaat
     2761 taacaatcat agataagaaa tatcattata tatttcacag gaatttataa aataagattt
     2821 aaaagatata aaaatatta
//
LOCUS       DAOSUD010000148         2684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_15337_length_2684_cov_26.737162, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000148 DAOSUD010000000
VERSION     DAOSUD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15337_length_2684_cov_26.737162"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..891)
                     /locus_tag="PLG49_10855"
     CDS             complement(217..891)
                     /locus_tag="PLG49_10855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOQ17842.1"
                     /translation="MRTKDEKQQGLSQLPGKSILYSKYLKRPLDFILALFALIIFSPF
                     LILIAVLVRIKLGSPVIFKQKRPGLNEKIFTLYKFRTMTDEKDEKGELLPDEIRLTKF
                     GKFLRSTSLDELPELFNILKGDMSFVGPRPLLVQYLPLYNEHQKRRHEVRPGLSGWAQ
                     VNGRNAISWEEKFDYDVEYVDHISFILDCKIIFMTIKKVLKREGISSETSVTMEPFKG
                     SKVNYK"
     gene            complement(891..2003)
                     /locus_tag="PLG49_10860"
     CDS             complement(891..2003)
                     /locus_tag="PLG49_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ17843.1"
                     /translation="MAKRIYLSSPHMSDESYEKAYIQEAFDTNGIAPLGKNVDEFEKE
                     LAAKVGIKAAAALSSGTAAIHMALKAAGVGEGDIVLCQDLTFSASANPIIYQNATPVF
                     IDSDYETWNMDPKKLEEAFEKYPNAKAVVVVHLYGLAANLDEIISLCKKHHAMLIEDA
                     AESLGTLYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNNEERIAKVRFWATQARD
                     PARYYQHSELGYNYRMSNVVAGIGRGQLKVLDKRVEKKKYIFEFYKRELEHLEGLEMM
                     PVNEWNQPNFWLSCIILRGKVRPINVIEALEAENIESRPIWKPMHMQPFYEKYDFIGE
                     GVSEDIFNNGVCLPSDTKMTDEDLKRVCDIIKGLWN"
     gene            complement(2055..>2684)
                     /locus_tag="PLG49_10865"
     CDS             complement(2055..>2684)
                     /locus_tag="PLG49_10865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ17844.1"
                     /translation="VGVDINVFNPERFNEDIKNRLRQELKIQKKPLIGFVGRMIREKG
                     VLELVEAFVKVRKAIPCQLLLVGSAELGERDENTLKQIKAYIADNKLEQDIIMPGHRE
                     DIPELLSIMDIFVLPSYREGMPVSLLEAMAMELPVVATDIRGCREEVDGTCGILVPKA
                     DVNSLVKALEYLLKNPLEAKAKGKNGRKRVLDLFTIEESVKKQINYFKD"
BASE COUNT          767 a          590 c          392 g          935 t
ORIGIN      
        1 cttataactt caataacaat attatgagaa taacttcctt caagtaaaac tctatcacct
       61 gctattccga ttcctaatat aggattttta gcaattgcct caataattaa atctgttatt
      121 acctcttttg tcaaagtcat cacaaaagtg agctatcatt aacacatctt tagccattat
      181 tattctcctt atttatcatg ataaataact tataaattat ttataattta ctttacttcc
      241 cttaaacggt tccatggtca ctgacgtttc tgaactgatt ccttctcttt tcagcacctt
      301 ttttatcgtc atgaaaatga ttttacaatc cagtataaaa cttatatgat ctacatactc
      361 aacatcataa tcaaattttt cttcccagct gatggcattc cggccattta cctgggccca
      421 gccggataag ccaggtctta cttcatgccg gcgtttttgg tgttcgttat acaagggtaa
      481 gtactgcacc agtaaaggtc tgggacctac aaaactcata tctcctttta gaatattaaa
      541 tagttccgga agttcgtcta aggaagttga ccttaagaac ttaccaaatt tagttaaccg
      601 gatttcatct gggagtaatt cacctttttc atccttttcg tcagtcatgg ttctaaattt
      661 atacaaagta aatatttttt catttaaacc aggtcttttt tgtttaaaga ttactggact
      721 gcccagttta atccttacca gtacagcgat aagtattaaa aaagggctga aaatgataag
      781 ggcaaaaaga gcaaggataa aatccaaagg cctttttaaa tatttgctgt aaagtatgct
      841 cttcccgggc agttgggata agccttgttg tttttcatcc ttagtacgca ttaattccac
      901 aaccctttta taatatcaca aacacgtttt aaatcttcat cggtcatttt tgtatcactt
      961 ggaaggcata cgccattatt gaatatatcc tcagaaactc cttcaccgat aaaatcatat
     1021 ttttcataga aaggctgcat atgcatcggt ttccatatag gtctggattc aatattttcg
     1081 gcttccaaag cttctataac gtttataggt ctgactttac ctcttaaaat aatacagctt
     1141 agccaaaagt ttggctgatt ccactcattt actggcatca tttccaaacc ttcaaggtgt
     1201 tcaagttctc ttttataaaa ctcaaaaata tattttttct tttctaccct tttatcaagt
     1261 acttttagct ggcctcttcc tatgcctgct accacattac tcatacgata attgtaaccc
     1321 aattcactgt gttgataata tcttgccgga tctctggcct gtgtagccca aaatctcact
     1381 tttgctattc tttcttcatt gttgctgacc aacataccac caccagaagt ggtaataatt
     1441 ttattaccgt taaaggaata aattccgtaa tcaccaaagg tccctgtata tttgccctta
     1501 tacaaggttc ctaatgattc tgccgcatct tctataagca tagcatgatg ctttttacac
     1561 aaacttatta tttcatccag atttgcagca agaccgtaaa gatgaacaac tacaacggcc
     1621 tttgcattcg gatatttttc aaaggcttct tctaattttt tggggtccat attccatgtt
     1681 tcatagtcac tgtctatgaa cactggtgta gcattttgat aaataatggg attggctgag
     1741 gcagaaaaag ttaaatcctg acaaaggaca atatctcctt cgcccactcc tgccgccttt
     1801 aaagccatat ggatagctgc tgttcctgaa gataacgctg ctgcagcttt gattccaacc
     1861 tttgctgcca attctttttc aaattcatcg acgtttttac caagaggggc tatcccattg
     1921 gtatcgaatg cttcttgtat atatgctttt tcatagcttt catcactcat atgaggcgat
     1981 gaaagataga tcctttttgc catcttaatt ctcctttaca ttagctcgtt ttaagctgtt
     2041 aaatgaacgc tcacttaatc tttaaaataa ttgatttgtt tttttacaga ttcctctata
     2101 gtaaacaagt ccaagactct ttttcttcca ttttttcctt tagcctttgc ttccagagga
     2161 tttttcagta aatactccag tgcttttact aagctgttaa catcagcctt tggaaccaaa
     2221 ataccacaag ttccatcaac ttcttctctg cagcctctga tatccgtagc caccactggc
     2281 agttccatgg ccatagcctc cagcaaggat actggcatgc cttcacgata agaaggaagg
     2341 acaaaaatat ccatgatact taatagttct ggaatatctt ccctatgacc aggcataatg
     2401 atatcttgtt ctaatttatt gtctgcaata tacgctttta tttgcttcag ggtattttcg
     2461 tctctttcac ccaactctgc agaacccacc agcagaagct gacagggaat cgcttttctt
     2521 actttaacaa aagcttctac cagttccaaa accccttttt ctcttatcat tcgcccgaca
     2581 aaaccaatca agggcttttt ttgtatcttt aattcctgac gcagacgatt tttaatgtcc
     2641 tcattaaagc gctcaggatt aaagacattg atgtcaacac caac
//
LOCUS       DAOSUD010000149         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_15601_length_2650_cov_22.141811, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000149 DAOSUD010000000
VERSION     DAOSUD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15601_length_2650_cov_22.141811"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..505)
                     /locus_tag="PLG49_10870"
     CDS             complement(35..505)
                     /locus_tag="PLG49_10870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17845.1"
                     /translation="MRYELAGKEELLKIHNIVQQTIKTVYPKYYPKEVVEFFCEHHNQ
                     DAILKDIEERRVGVLRVNDEIVATGSYENDHITRVYVLPKHQNKGYGTFIIKSIESEI
                     SKKYHKAFLDASLPGVHLYEKLGYKTLEHKKYFVKNDVVLVYDIMVKELYRHSL"
     gene            complement(1403..>2650)
                     /locus_tag="PLG49_10875"
     CDS             complement(1403..>2650)
                     /locus_tag="PLG49_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ17846.1"
                     /translation="DITGLYNTKKLFYDLTDLIEKGNRFTIISVKLTNIEGIGKYVDF
                     NLVQKLIKLLISDMKAKFEHLSMYSSSDDELILILPDDCQYFDKIKDLVNKYSTAVKI
                     DGFEVRMPLKAGIYNFGGKTESPIEIYNKSRVASEQGEINESGIYYYDVQIDNERKEL
                     YEIAGSLHKALNNNEFYLVYQPKINIQENKISGVEVLLRWDRGDKKPVGPGVFIDVAE
                     KIGLIRDISKYVIENVIKQIKIWEKENISIHCSINLTAQELTDQELIEWAVGKIESHD
                     IDRSMIEIEITERVLNKEFEKINKHLEYLREIGYKISIDDFGTGYNSLMMIGMIHYDV
                     LKIDKFFLDRMCNFENRELVRKIIDYVHVLDKKVVAEGVETEEQLNFLKEYGCDEVQG
                     YYFSKPLLAEDFAKFYKEFYKMN"
BASE COUNT          861 a          504 c          310 g          975 t
ORIGIN      
        1 ttcagaattg atttcaggaa taaaaatttt tccgtcataa tgaatgtctg tacaattcct
       61 ttaccataat atcgtaaacc agtactacat cattttttac gaaatatttt ttatgctcta
      121 atgttttata acccagtttt tcatacaaat gaacgccagg caaggaagca tccaaaaaag
      181 ctttatgata ttttttgctt atttcagatt caatagattt tataataaat gtaccataac
      241 ccttattctg atgttttggc aacacataaa ccctggtaat atgatcattt tcataacttc
      301 ctgtagccac aatttcatca tttactctaa ggactcctac ccgtctctct tctatgtctt
      361 ttaatatggc gtcctgatta tgatgttcac aaaaaaattc aacgacttcc ttcgggtaat
      421 attttggata aaccgttttt atggtttgct ggacaatatt atgtattttt agtagttctt
      481 ctttccctgc taattcatat ctcatctttt tccctctcca acacatcaga ttttgtaacc
      541 aaaaccttaa agattaagta agccgttatg cccaaatata atctgtacag tttataaatc
      601 tcacatttgt aatatttaat gactcaaaaa gaacctttac tttttcagtg acagttattc
      661 caaaagtatc tatatgataa aaaaaatctg agccatccca tgaatcaagg ggaaaaaaat
      721 accctttttg tttaattatt ttcgtttgat ttggcaatat aacctctttt ttttcagcta
      781 aatttctatc cgccttaagt gcttttccaa taatataaaa ggcatgataa tttttaattt
      841 tttcattagt ttttgtatga atgattaccg gatatgaccc ccatccagtt atactatatt
      901 cttttaatgc tttaacaacc ttatctgata ctaaataaaa tgcaggagca cccgcaacaa
      961 taaaatcaaa agcctttttg ccgccgactt caacaaaaat ttctggcctt atatttataa
     1021 aatccccaca aaaaattttt tacggtcata tgttgcaatt tcactgttat ttataggtgc
     1081 gacagtcaga gcacagttca acattgcatc tgaaaaacaa taaaaatgtt catactgcgg
     1141 cataaaacac atgctaacca tctactcccc ttatttaaca gaaaattaaa ttcatactga
     1201 tttttaagta ttttaacata tcttttttcc ccctgcaaag aaaattccat aaagtttttt
     1261 ccaggcatgc taatcattat aaatgaaaaa tgaggacttc agttcaggct ttccttgata
     1321 tataaaagcc tctaaaaaat ttgaagtcct caaacataaa gggtgtgtct gtaattgtaa
     1381 ataagaaaga aatcatattc acttaattca ttttataaaa ctctttataa aattttgcaa
     1441 aatcttctgc taataaaggt ttactaaagt aatagccttg tacctcatca catccatatt
     1501 cctttaaaaa attaagttgt tcttctgttt caacaccttc tgcaacaact tttttatcca
     1561 atacatggac ataatctatt atttttcgaa ccaattccct gttttcaaag ttgcacattc
     1621 tgtctaagaa aaatttgtca atttttaaga catcataatg gatcatacca atcatcatta
     1681 atgaattata acccgtacca aaatcgtcaa tggatatttt ataaccaatt tctctcagat
     1741 actcaaggtg cttattaatt ttttcaaatt ctttatttaa aaccctttct gttatttcaa
     1801 tttcaatcat ggatctgtca atatcatggc tttcaatctt ccctacggcc cattcaatta
     1861 attcttgatc cgtcagttcc tgagctgtta agttaataga acaatggata gaaatatttt
     1921 ctttttccca tatttttatc tgtttaataa cattttcgat gacatacttt gatatatccc
     1981 taattaaccc aattttttca gcaacatcta taaatacacc aggacctaca ggttttttat
     2041 ctcccctgtc ccatcggagt aatacttcaa cccccgatat tttattttcc tgaatattaa
     2101 ttttgggctg gtatacaaga taaaattcat tgttatttaa agccttatgc aatgaacctg
     2161 cgatttcata caattctttt cgttcattat cgatctgaac gtcataatag taaattcccg
     2221 actcattaat ttctccctgc tcactggcta ctctggattt attataaatt tcaatgggag
     2281 actcagtttt tcctccaaaa ttgtaaatgc ctgccttaag aggcatccta acctcaaaac
     2341 catcaatttt aactgccgta gaatatttat ttaccagatc ttttatttta tcaaaatact
     2401 ggcagtcatc aggaagaatt aatattaatt cgtcatcaga agaggaatac atagacagat
     2461 gttcaaattt agctttcata tcgcttataa gaagctttat aagtttttga accagattga
     2521 aatcgacgta tttccctatt ccttcaatat tcgttaattt tacagaaata attgtaaagc
     2581 ggtttccttt ttcaattaaa tccgtcagat cataaaaaag tttcttagta ttgtataggc
     2641 cagtaatatc
//
LOCUS       DAOSUD010000150         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_16110_length_2591_cov_25.906940, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000150 DAOSUD010000000
VERSION     DAOSUD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16110_length_2591_cov_25.906940"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1361
                     /locus_tag="PLG49_10880"
     CDS             144..1361
                     /locus_tag="PLG49_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOQ17847.1"
                     /translation="MSTLSKEQVKELVKGNNFQTVSDVSAYLKDIFKDIIQELLEAEL
                     ETKLGYAKDDVENKNTDNSRNGYSSKTIKSEFGEVDIQVPRDRKGEFRPKIIPKYQRD
                     VSGIEEKVMALYARGMSTRDISEQIQELYGFSLSAEMVSKITDRIAPEIKQWQQRPLE
                     PIYPFIFMDAIHYKVRDDGRIVNRAAYVVLGVSAEGSKDILGIWIGDNESSKFWLGVL
                     NDLKNRGVQDVLIFCVDGLTGIKEAINAAYPKAEVQRCIIHQLRNSFKYVSYKDVKAF
                     SNDFKEVYRAINEEVALEKFCELKSKWGKEYPYAIRSWENNWDVISPFYKFSEEIRKI
                     IYTTNIIEGLHRQFRKVTKTKTMFPSDSSLEKMLYLASMNVTKKWTQRYKNWDRVLSQ
                     LMIQYPGRLESYI"
     gene            complement(1622..1927)
                     /locus_tag="PLG49_10885"
     CDS             complement(1622..1927)
                     /locus_tag="PLG49_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOQ17848.1"
                     /translation="MNKANINNCTIVQDLLPLYYDNVCSEESKELVREHITECPTCRK
                     IADELNNKNSEKRLSNEAGNVLKRHARRETKKSAIVGGVVAGILMISLIVCLIWEYK"
BASE COUNT          923 a          427 c          448 g          793 t
ORIGIN      
        1 gatacaaaac ttacaaatgc agttcttgag ggaataaata gtattataca aactgagaat
       61 gtctaaaatt ttgtgtataa gagaatcaaa agcctccttc ttggcaagaa ttatagaaat
      121 aataaccaag gaggagattt tttatgtcaa cactatcaaa agagcaggta aaggaattag
      181 ttaagggaaa taattttcag actgtttcag atgtgagtgc atatttgaaa gatattttca
      241 aggacattat tcaagagctt cttgaagcag aacttgaaac aaaattgggt tatgcaaaag
      301 atgatgttga aaacaaaaat acagataata gtcgaaacgg atattcatca aagaccataa
      361 aaagcgagtt tggagaagtt gatattcaag taccaagaga ccgaaaagga gagtttcggc
      421 cgaaaattat acctaagtac cagcgagatg tttcaggaat tgaagaaaaa gttatggctt
      481 tatacgctag aggaatgtct acacgagata ttagtgagca aattcaagaa ctatatggct
      541 ttagtttgtc agctgaaatg gttagcaaga tcacagacag aatagctccg gagatcaagc
      601 agtggcaaca aaggccgcta gagcctatat atccgttcat ttttatggat gcaattcact
      661 acaaggttcg agatgacgga agaatagtaa atcgtgcagc atatgttgtt cttggtgttt
      721 cagctgaagg aagtaaagat attctaggaa tatggattgg tgataacgaa tcatctaaat
      781 tttggcttgg agtcttaaat gaccttaaga acagaggtgt acaggatgtt ctaatatttt
      841 gtgttgatgg actgacaggc ataaaagaag caattaatgc agcatatcca aaggccgagg
      901 tgcagagatg cataatacat cagcttcgaa actcttttaa atatgtgtca tataaagatg
      961 ttaaagcttt tagtaatgac tttaaggaag tataccgtgc tattaacgaa gaagtagcgt
     1021 tagagaagtt ttgtgaactt aagagcaaat ggggaaagga atatccttat gcaatacgaa
     1081 gttgggaaaa taattgggat gtgataagtc cgttttacaa attttcagaa gaaatcagaa
     1141 aaataattta tactacgaat ataattgaag gactgcatcg acagttccgt aaagttacga
     1201 aaacaaaaac aatgttccca agcgacagtt cacttgaaaa gatgttgtat ttagcatcga
     1261 tgaatgttac aaaaaagtgg actcaacgct ataagaattg ggatcgggtg ctaagccaat
     1321 tgatgatcca gtatccaggt agattggaaa gttatattta aaagcgttca cccccaccgc
     1381 cctcaagggc tcgtcctcat tgccggacgg gctagacccc acaaattgaa aaacggtcga
     1441 aaaaatgagc ggaatgcacc attatgaata attattgcca gattaaatca agtttatgta
     1501 taaaacttaa taaatctggc aataatatat aatgtaaaga tagttaagag taatattctg
     1561 taaaccttaa aaacttgaaa ttacttgtct agaaggattg cataaaattc catacacaga
     1621 attatttata ttcccaaatc agacatacta tcagcgaaat catcagaatg cctgccacaa
     1681 cacctccaac tatagctgac tttttcgtct ctcttcgggc atgtcttttc aaaacgtttc
     1741 cggcttcatt actcaaccgt ttctctgaat ttttattatt taattcatcg gcaattttcc
     1801 tgcatgttgg gcactctgta atatgctctc ttaccagttc cttactctct tcgctacata
     1861 cattatcata atatagtggc aggagatcct gtacgattgt gcaattattt atatttgctt
     1921 tattcatctg ttttcatcct ttccactagt ttcagtcatt gacggtaata tgtcactctg
     1981 accaaacttt ctgtttttcg aaatacattg caaatattct actctcttca tattatttta
     2041 tcaaacttgc actgattttt gtgttttatc ctatattgtt tcgttatcaa atatccatag
     2101 tagaaccata tataaaaaaa caataggaca aggctccaaa ttggatagtc tattattata
     2161 gtttcataac taatgcgatt attactatag tcagcgaaaa aacgctacat actaataacc
     2221 acttgttttt tctatagaga attaagttta tgaatgacga tagaaaaaag gaataatcca
     2281 ttataatatc aaaacactta ctatcagaat ccacttaagt gccattcctt cataaaaatc
     2341 ccccataatt gcagacgttg cagctaagaa aacaattata ttcagtatat ttacttattt
     2401 tcattatact tttcctttcg taatcgtctt gataagaagg cggataatct ttatccttta
     2461 atcaaataaa tatcataaac ttcaaaactt agtaaaccgc tatctcccca ctggacagac
     2521 tcattaacaa cctaataaaa aaaaacagta ccgaaaaaaa tcaattttaa tttgtatctt
     2581 tattatcata t
//
LOCUS       DAOSUD010000151         2588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_16138_length_2588_cov_21.311488, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000151 DAOSUD010000000
VERSION     DAOSUD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16138_length_2588_cov_21.311488"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..741
                     /gene="smpB"
                     /locus_tag="PLG49_10890"
     CDS             268..741
                     /gene="smpB"
                     /locus_tag="PLG49_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ17849.1"
                     /translation="MTKTNSDIKIIAQNKKAYHDYFIEDTYEAGISLTGTEVKSVRMG
                     KVSIKESYIRIKDGEVFILNMHINPYEKGNIFNKDPLRTRKLLLHKREISKLMGAVAQ
                     KGYTIVPLKVYLKGSLVKVLIGVAKGKKLYDKREDIAKKDQRRQAEKEFKVKNLY"
     gene            751..1102
                     /gene="ssrA"
                     /locus_tag="PLG49_10895"
     tmRNA           751..1102
                     /gene="ssrA"
                     /locus_tag="PLG49_10895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1334..2026
                     /locus_tag="PLG49_10900"
     CDS             1334..2026
                     /locus_tag="PLG49_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOQ17850.1"
                     /translation="MKTIGLIGGMSWESTMVYYQLINEVVKNQLGGLHSAKLLLYSID
                     FYEIEQCQVKGEWDKAADILSAVAQNLEKAGADFIVICTNTMHKVVPQIQGRIKIPVL
                     HIAEATAEELKAHNIVKVGLLGTKYTMTQEFYKEKLMSEGIEVVIPNDDDIERINDVI
                     YHELCLGIISDQSKQEYLRIINQMAKKGADGILLACTEIGMLIKQEDTSLPVFDTTQI
                     HARRAALFAIND"
     gene            complement(2495..>2588)
                     /locus_tag="PLG49_10905"
     CDS             complement(2495..>2588)
                     /locus_tag="PLG49_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ17851.1"
                     /translation="LALREWKCPNCGAIHDRDVNAAKNLLKLAM"
BASE COUNT          916 a          383 c          493 g          796 t
ORIGIN      
        1 atttcctttt tagcttctgg tatttggctt ccatagaaac aaacatggtt tttttcgtat
       61 taacataata acctcctaat tagttctatt aaatatagtt tattgcatct aataataaag
      121 tacaataacc atttttatgg aatcaaatta tgatgaaaga tattcaatta attgaaaaaa
      181 aaggtttctg caaagacttg aaatcgctta aaaatatgtt ataataaatc ttgcacccta
      241 aatgggaaga cgggggagaa tattcaaatg acaaagacaa attcagatat taagattatt
      301 gcacaaaata aaaaagcata tcatgattat tttatcgaag atacttatga agcggggatt
      361 tcccttacag gaacagaagt aaaatctgtc cgcatgggta aagtcagcat caaagaaagc
      421 tatatccgta taaaagacgg agaggttttc atattgaata tgcatattaa tccttatgaa
      481 aaaggcaaca ttttcaataa agatccccta agaacaagaa agttactgct tcataaaaga
      541 gaaatctcca agttgatggg tgcagttgct caaaaaggct ataccattgt tcctcttaaa
      601 gtatatttaa aaggaagctt agttaaagtt ttaattggtg tagcaaaagg taagaaatta
      661 tacgataaac gggaagacat agcaaagaag gatcaaaggc gtcaagctga aaaagaattt
      721 aaagttaaaa acttgtatta gcttatttac gggggtgtac tggtttcgac gggaatcacg
      781 tatatcgggg cagccattcg cagaatccgg agctgcgtca acaatctgga acttaaatat
      841 aaacgcagaa gaaaattacg cttttgctgc ttaattaaaa gcagctgtct ctcttagatt
      901 cccgcagtct aagaagaggc accgaccagg cgggcaactt tctctcttaa gctttgcgga
      961 gagatcgtat tgatgaagct accaaggtaa aaagcctgtc gttaggcgtt ttaccaaggg
     1021 aattttaaaa taacgactat aatggaagag accacgatat atggattttc ggacgggggt
     1081 tcaactcccc ccacctccat cgcatctatc cacgtcattt tttggcgtgg atttattaaa
     1141 ttttgtttcg tttccggtat ctatataaag aaataatgcc ttattggaga aaaattctct
     1201 aataaggctt ttttgaactc gttctgacag ttaagaaagg tatttatttt attaaatttt
     1261 atgctagact tgataagaaa aaatttatat tttaataaag tggttaaaga ataatttatg
     1321 gagggataat ctattgaaga caatcggttt aatcggaggt atgagttggg aaagtacgat
     1381 ggtatattat caactgataa atgaagtcgt aaaaaatcaa ctcggaggtt tgcattctgc
     1441 aaaactactt ttatacagca ttgattttta tgaaatagaa cagtgtcagg ttaaaggaga
     1501 atgggataaa gctgctgata ttttatctgc tgttgcgcaa aaccttgaga aagctggtgc
     1561 agattttatt gtgatttgca ccaatacaat gcataaagtc gttccacaaa ttcaaggcag
     1621 aataaagatt ccagttttac atattgctga agcgacagca gaggaattaa aagctcataa
     1681 catagttaaa gtaggtctat tgggaacaaa atatactatg acccaggagt tctataaaga
     1741 aaaattaatg agtgaaggaa ttgaagtggt gattcctaat gatgatgaca ttgaacggat
     1801 taatgatgtg atatatcatg aattatgctt aggaatcatt tcagaccagt cgaaacagga
     1861 atatttgcgt attattaatc aaatggcaaa gaagggagca gatggaattc ttttagcttg
     1921 tactgaaatt ggaatgctga ttaaacaaga agatacatct ttgcctgttt tcgatacaac
     1981 tcaaattcat gcaagaagag cagccttgtt tgccattaat gattaaagaa agaataaaat
     2041 gacataagag cagatatttc aaagatagat tatatgaatc atgttaaaaa ggtcttttag
     2101 aaacttgatt ttgttcttgt tgtatatcat tttaacggat ttatttcaaa gtacatttaa
     2161 atatcgggct atagattaaa tttgtcccag gcgctatgta aatcattctt ttgataatag
     2221 cgtctttttt tatgaactac attattgaaa gtttatttta ttaagataca gtaacatcaa
     2281 tgaaatgccc taatcataaa atattaaata gaatgtgttt tatgggggga agaaatgaaa
     2341 ataacagaaa aatttttgga gaaaaatgtg tatagcagac caggaacaaa agtgaactac
     2401 cgccacttat agaagtggca gcttcgtggt caaggtaact accgttacca gataccccac
     2461 gctcaaaggg ctgttccttc cccaggctta ccatctacat ggctaatttt agtaggttct
     2521 ttgctgcgtt tacatctcta tcatgtatag caccacaatt tgggcatttc cattctctta
     2581 gtgctaaa
//
LOCUS       DAOSUD010000152         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_16573_length_2533_cov_24.729217, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000152 DAOSUD010000000
VERSION     DAOSUD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16573_length_2533_cov_24.729217"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLG49_10910"
     CDS             <1..958
                     /locus_tag="PLG49_10910"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOQ17852.1"
                     /translation="KLLPLILMATLLLSACDPNKLQFFINAKTGDNYTYNVTIDQTTK
                     SEILGLETRTKQTISYDYSVNVDDVKSNGDLELTYTYDRIRFNMNMDDIEVSFDSDSA
                     TSGDEISKIYKSFIGKQFNVIMTKYGEIKEVKGMDEFLDTLTEPFKSGNTDIFGLDYL
                     NQLKDSLSGHFSEESIKNDMGLITRYMPKKKVNVGDSWTIKESIDKSNIKIDAEITYT
                     LEKLEDEIAYIIISGTYSTNEPSKISYDALKANVNLEGNIEGNIQADINNGFIFEGEI
                     TQNIKSNMDFGAMDLSKLGIEGNLEIPMETTLIMKFSSKKAN"
     gene            complement(1351..>2533)
                     /locus_tag="PLG49_10915"
     CDS             complement(1351..>2533)
                     /locus_tag="PLG49_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOQ17853.1"
                     /translation="KDSLMSEMKVVRDGVIVFVSICIIIAVLVGLIISLSISKQLKNL
                     MHLMGKVEQGDLTVNASIKGNNELGKLSQSFNQMIGNVRNLIVKTEEVVKEVEENTNI
                     IRNSSNQSAQAAAQVANAVNELAEGSTEQARQADNTNALMEKLSEEINRSVRRINDII
                     NTIQEMEKSRDYLNNTIVQLNDKTKIVIESSNIINEKINNLSEETKEVIKVVQVIDSI
                     SEQTNLLALNAAIEAARAGEAGKGFSVVADEIRKLSVETKASTEIIRKIIANIGEKTL
                     SAVSTVEDSEKIFNEEKAIVLKAQDAFEEMAVLIKKVVEQIEDINQNLAVIEEQKNQT
                     IEATGHIAAIIEESAASIEEVTATSEEQTSSAEQLAYLAEKLTHAVENLKDTLLMFKI
                     K"
BASE COUNT          837 a          426 c          358 g          912 t
ORIGIN      
        1 aaaactattg cctttgatcc tgatggcaac acttttgtta tctgcttgtg acccaaataa
       61 attgcaattt tttatcaatg ctaaaacagg ggacaactat acatacaatg tcaccataga
      121 tcaaacaaca aaatctgaaa ttttgggact ggaaacgaga acaaaacaaa ccatatccta
      181 tgattattct gttaatgtgg atgatgttaa atcaaatggt gacttagaat taacttacac
      241 ctatgaccga atccgtttta acatgaatat ggatgatata gaagtttcat ttgattctga
      301 ctctgctact tcaggcgatg aaataagtaa aatatataaa agttttattg gaaaacaatt
      361 taatgttata atgacaaaat acggtgaaat aaaagaagtt aaaggcatgg atgaatttct
      421 tgatacatta actgaaccat ttaaatctgg caatactgat atttttggtt tagactattt
      481 aaatcaatta aaagattccc tttcaggcca ttttagtgaa gaatccatca aaaatgacat
      541 gggacttatt acaagataca tgcctaagaa aaaagtaaat gtaggagatt cctggactat
      601 taaagaaagt attgataagt caaatataaa aattgatgct gaaatcactt atactctgga
      661 aaaattagaa gatgaaattg cttatattat aataagcggc acatattcaa ccaatgagcc
      721 ttctaagatt agttatgatg cattaaaagc taatgtaaat ctggaaggaa atattgaagg
      781 aaatattcag gcagatatta ataatggttt catttttgaa ggtgaaatca cacaaaatat
      841 aaaaagtaat atggattttg gagctatgga cttaagtaag ttaggtatcg aaggcaattt
      901 agagatccct atggaaacta ctctaattat gaaattttct tcaaaaaaag ccaattaatt
      961 aagcttattt acaatttcat cataaatgat ataatatgat aaaggaagta gccttagctg
     1021 cttcctttta taccctattc acttattaca gatatatcaa attgatttga tattcggtag
     1081 tatgttttga aaatagggtt ttttatataa aatttatgcg ttaaaccaaa tactttttgt
     1141 gcctccaaaa cattaaattc caaaaaacaa agtgaaagac tgtctttcta ttgaaaggaa
     1201 tgaaataaaa ttaactgctc attcatgtaa aagtgaatag ggtataatta aaagccggtt
     1261 agaaaatttc ttccctaacc ggctttaatt tttctttatt ataaatccgt atcaactttt
     1321 ttcttgcttt cctataatga aaaagaaata ttatttaatt ttaaacatta ataaagtgtc
     1381 tttgagattt tccaccgcat gtgtcaactt ttctgctaaa taagccagtt gctccgcaga
     1441 actggtttgt tcttcactgg tagcagtaac ttcttctatt gaagctgcag attcttcaat
     1501 tatagctgct atgtgccctg ttgcttcaat ggtttgattt ttctgttctt ctattacagc
     1561 cagattttga ttgatatcct caatttgctc aacaactttt ttaatcaata ctgccatttc
     1621 ttcaaaagca tcctgagcct tcaaaacaat ggctttttct tcgttaaata ttttttcaga
     1681 atcttctaca gtcgatacag cactaagggt cttttcacca atattcgcta taatttttct
     1741 aattatttct gtagaagctt ttgtttcaac agataatttt ctgatttcat ctgccactac
     1801 agaaaatcct ttgcctgcct ctcctgctct tgccgcttct atagcagcat ttagcgccag
     1861 caggttggtt tgttcactaa tagaatcaat cacttgaaca acttttatca cttcttttgt
     1921 ttcttcactt aagttattta ttttttcatt aataatattg gatgattcta taacaatttt
     1981 tgttttatcg tttaactgaa caatcgtgtt attcagataa tctctggatt tttccatttc
     2041 ctgaatcgta ttgataatat cattaatccg tctgacagaa cgattaattt cctctgacag
     2101 tttttccatt aatgcatttg tattgtctgc ctgccttgct tgctcggttg aaccttccgc
     2161 aagttcgttt acagcatttg caacttgagc cgcggcttgt gcggactgat tagaactatt
     2221 tctaatgata ttggtatttt cctccacctc ttttaccact tcttctgtct taacaataag
     2281 atttctgacg tttcctatca tctgattaaa gctttgagat aattttccta actcattatt
     2341 tcctttaatt gaggcattta cagttaaatc accctgttca acttttccca ttaaatgcat
     2401 taaatttttt aattgttttg aaatacttaa agaaataatc aaacccacta acacagcaat
     2461 aataatacaa atgctaacaa atacaataac gccgtctcta actactttca tctcactcat
     2521 taatgaatct tta
//
LOCUS       DAOSUD010000153         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_17140_length_2472_cov_34.396359, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000153 DAOSUD010000000
VERSION     DAOSUD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17140_length_2472_cov_34.396359"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..665)
                     /locus_tag="PLG49_10920"
     CDS             complement(126..665)
                     /locus_tag="PLG49_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17854.1"
                     /translation="MSSNQETELSQNQEKGKQASVSGFAHELIAAGILMKRYQNVSLV
                     DLPLSPYDLIIVLKAEDGSENIIRAQVKTATKSISFTGGTRGGVDREYKSGVKIYRQS
                     KKTSDVVIGVTPRLNDEGDKGYDLYFVPTILIEKLNQNSISINKIPFLKNNYDMLKKC
                     KDENEVSSLYEKYKHLLDK"
     gene            complement(687..2087)
                     /locus_tag="PLG49_10925"
     CDS             complement(687..2087)
                     /locus_tag="PLG49_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOQ17855.1"
                     /translation="MQVSDYYPISLDEIEAKVLKKLKRNWMQKCEDVEPLDNVKEITN
                     DFGEKIIRVDGEIPVDLPINNGDRFLFISYDQSSLTHGLHKYPAKFFPELPRWLIKRY
                     SKDKDLVIDPFMGSATTNVEALLARRHSVGIDISPFSRFLAKVKVTPINEQELERCKK
                     GMLRLLVNYRPEKVANEDIPTFPYRDNWFNKEIILELAYIKKCIENLDTSEDIKDFFK
                     ICFSSIIRSVSNADDNCTRTVIRKKLKKTVNPSDALRRFAEVILTNIPKMIEFSNLCP
                     KNISVTFPEDMDARNIKYENETFDLALTSPPYANAVDYPRMHQLETYWLGFAKGSLAT
                     LKKKQVGTESVTAKKYKDLHLTGIKEADKVITRIYNKDPRRAYIAYKYLMDMETNLKE
                     IYRVLKSNGKYVIVVGNNKIRGEVFENWKYIISMAENIGFVLENYFASEIIKHFIKVP
                     REERIDTDWILVLTKR"
BASE COUNT          725 a          456 c          398 g          893 t
ORIGIN      
        1 aagtcccctc aataagcttg tgtcggcgag gaagagggtg gggagggtga aagatgcgaa
       61 gaaataagag ataacgttgg attaatatca aggttgtttc aatttgtctg gcatattttt
      121 attttttatt tatctagtaa atgtttatat ttctcatata aagatgagac ctcattttca
      181 tccttgcatt tttttaacat atcgtaattg ttctttaaaa atggtatttt attgattgag
      241 atactattct ggttaagttt ttcaattaat attgtgggaa caaagtaaag atcatatccc
      301 ttatctccct catcattcaa tcgaggagtt actccgatga caacatcgga tgttttttta
      361 gattgtctat aaatcttaac gcctgatttg tattccctat cgactccgcc cctagtgcca
      421 ccagtgaaag atatagattt agtagcagtt tttacctgag cgcggataat attttctgaa
      481 ccatcttcag cttttagaac tatgattaag tcgtagggcg atagaggcaa atccactaag
      541 gagacatttt ggtatcgctt cattaaaatc ccagcagcta taagctcatg ggcaaatcca
      601 cttactgatg cttgtttccc cttttcttga ttttggctca gttcggtttc ttgattgcta
      661 ctcattttaa tccactacct cgctttctat cttttcgtta acaccaaaat ccaatctgta
      721 tctatacgct cttctcgtgg tactttaatg aaatgcttaa taatttctga cgcgaaataa
      781 ttttctaata caaatcctat attttctgcc atcgaaatta tatatttcca attttcaaaa
      841 acttcacctc ttattttatt attgccgaca actataacgt attttccatt ggacttcaaa
      901 actctgtaaa tttcttttaa gttggtttcc atatccatca aatacttata agctatgtat
      961 gctctgcgag ggtccttgtt atatatcctt gtaatcactt tatcggcctc ttttatccca
     1021 gttaagtgta aatctttgta ctttttagca gttacacttt ccgttcctac ctgttttttc
     1081 tttaatgttg caagagagcc cttagcaaaa cctaaccaat aagtttctag ctgatgcatc
     1141 cttggatagt ccactgcatt agcataaggt ggagaagtta gtgcaagatc aaaagtttca
     1201 ttttcgtatt ttatatttct cgcatccata tcttcgggaa aagtaacgct tatatttttg
     1261 gggcacaaat tagaaaattc tatcatttta gggatatttg taagaatgac ctctgcaaac
     1321 ctcctgagtg catcagaggg gttcactgtt tttttaagct ttttgcgtat cactgttcta
     1381 gtgcaattgt catcggcatt ggaaacggag cgaattatag atgaaaaaca tattttaaaa
     1441 aaatctttaa tatcttcact agtatctaaa ttttcaatgc attttttaat atatgctaat
     1501 tctaatatga tttctttatt aaaccaatta tcacgataag gaaaggtagg tatatcttca
     1561 ttagctactt tttcggggcg ataattcacc aataggcgta acattccttt tttgcacctt
     1621 tcaagctctt gttcatttat cggcgtaact ttgaccttag cgagaaacct ggaaaaggga
     1681 gaaatgtcta tgccaacaga atgtcttctt gctaaaagtg cttctacatt tgttgtggca
     1741 ctgcccataa atgggtcaat aactaagtct ttatcttttg aatagcgttt tattaaccaa
     1801 cgtggaagtt cgggaaaaaa tttagctgga tatttatgca aaccatgcgt caaactagat
     1861 tgatcataac ttatgaacaa aaatctatct ccattgttaa ttggtagatc gaccggtatc
     1921 tcgccatcaa ctcgaataat tttctcacca aaatcatttg taatttcttt cacgttgtcc
     1981 agaggttcga catcttcgca tttttgcatc cagtttcgtt ttagtttttt taagactttt
     2041 gcctctattt catcaagtga gataggataa taatctgata cctgcatttg aaagtattca
     2101 ccccttttat gtttagaatt tgatgtttac cgttttacta acataagaaa ttctagtcta
     2161 agcttgtgtt gcaagtaaaa cagctttaaa agaaatttaa tagacttaac attatcagcg
     2221 atactaaatt tagctacaaa atcacacaga tttattaaaa catacgctaa tactagaaag
     2281 gtattattgc cataactaag ggctattgaa atgatatcat gaatatgtcc aaattagaag
     2341 gtcaaaaatg tacaacatga ttgcttttcg cggcttcacc ccccaagaaa tttattttct
     2401 gggaaaggtg gggcggctca gtttcgcaaa ctctgccctt tttctatgag tccacagatc
     2461 atcaccattc ct
//
LOCUS       DAOSUD010000154         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_19096_length_2285_cov_23.545291, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000154 DAOSUD010000000
VERSION     DAOSUD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19096_length_2285_cov_23.545291"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1431)
                     /locus_tag="PLG49_10930"
     CDS             complement(88..1431)
                     /locus_tag="PLG49_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOQ17856.1"
                     /translation="MRKKFTLGIHFDVYSGRAVLLDISNGELVSSKVFEYPKKAGINQ
                     LPDGTSLGENCCLGHPSNYLYAIKSLISELLKDANVYPSQVIALGINFDSYTIISADN
                     EEPENIGGDSFLVEAPDWITMELTGELVSSSIYPLGEKIGELNQMTSMNTGLLVGTCL
                     AVPASNAHVTLPALGVTEAGEMVILLERSIRQFILSKEKKELPGLCSLIEDRLIPGYF
                     TYETMQPEAGEIFDSFLEQFVNKEYHEAAENEGIDLLTYLIKKAELLRPGESGLLVLD
                     WWSGNKSILDNKDLSGLIIGLGLKTKPEAIFRALMEAVAFGTLKIIKNFREYGIPVEK
                     LYLCGELVEKNPLLMQIFSDVCNMEIYTSAFSHPTAVGSAIMASVAAGKALGGYDNVF
                     EAAKKISKLNDTVYRPIAENVEIYKELFIEYEKLYNYFGHNENTIMKRLKEIKER"
     gene            complement(1591..2280)
                     /gene="araD"
                     /locus_tag="PLG49_10935"
     CDS             complement(1591..2280)
                     /gene="araD"
                     /locus_tag="PLG49_10935"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOQ17857.1"
                     /translation="MLTELKKIVLEANLALVRHNLVIFTWGNVSGIDREKGLVVIKPS
                     GVKYEELTEDKLVVVDLAGNIVEGSLRPSSDTPTHLVLYKHFPDIGGVVHTHSPWATS
                     WAQAGKSITAVGTTHGDYFYGNIPCTREMTAEEIEGNYEEETGKVVVETFRNLNPMEI
                     PGVLVHSHGPFSWGKDPLEAVHNSVVMEEVAKMTYHTMMLSPGKEAMDQKLLDKHFLR
                     KHGKNAYYGQN"
BASE COUNT          697 a          498 c          310 g          780 t
ORIGIN      
        1 atcaaacaag tatataattt ttctttcaat catagtatgc ctgatttcag tcaataattt
       61 actgggatag tctgccacgg aaaagtttta tctttctttt atttctttta accttttcat
      121 aatcgtattt tcattatgac caaaataatt ataaagtttt tcatattcta taaaaagctc
      181 tttgtaaatt tcaacatttt cagcaattgg tctgtaaaca gtatcgttta attttgatat
      241 cttttttgct gcctcaaaaa cattatcata tccacccaaa gctttacctg cagctactga
      301 agccataatt gcagaaccaa ccgcagtagg atgagaaaaa gcggaagtat atatttccat
      361 attgcataca tcactaaaaa tttgcataag caaaggattt ttttcgacca attccccgca
      421 aaggtataat ttctcaacgg gaattccata ttctctaaag ttttttataa tctttaatgt
      481 gccaaaagcc acagcctcca tcaatgccct aaagattgct tcaggttttg tctttaaacc
      541 taaaccaatg attaaaccac tcaaatcctt attatccaga atactcttat ttccgctcca
      601 ccaatcaagg acaagaagcc ctgattcacc aggtcttaat agttctgctt tctttattaa
      661 ataagttaaa agatcaatgc cttcattttc tgctgcttca tgatattctt tatttacaaa
      721 ttgttcaaga aagctgtcaa aaatctctcc tgcctctggc tgcattgttt cataagtaaa
      781 ataacctggt atcagtctgt cctcaatcag actacacaaa ccagggagtt ctttcttttc
      841 ttttgaaaga atgaactgtc tgatagacct ttccagaaga attaccattt ccccggcttc
      901 tgttacccca agagcaggaa gagtcacatg ggcattactg gctggaacag ctaaacaagt
      961 tccgacaaga agacctgtat tcatcgatgt catttggttt aattcgccga ttttttctcc
     1021 taaaggataa atggaactac ttaccaactc tcctgtaagt tccatcgtta tccagtcggg
     1081 cgcttcaact aaaaagctgt ccccacctat attctccggt tcctcattat ctgcagaaat
     1141 aattgtataa gaatcaaaat taatccctaa agcaatcact tgtgaaggat atacattagc
     1201 atcttttaat aattcagata ttaaagattt tatggcataa aggtaattag aaggatgacc
     1261 aaggcagcag ttttccccta aacttgttcc atccggaagt tgattgatac cagccttttt
     1321 aggatattca aatacttttg aagacactag ctctccattt gaaatatcca ataaaactgc
     1381 tcttcccgaa taaacatcaa aatgaattcc cagtgtaaat ttttttctca tgattctccc
     1441 cctaagtagc ttttaccatc aatctctgct cttttgcgac attttacatc cttcattaac
     1501 catttttaag gcatatcctt atatagtatt ttgtcattaa taagataata ttgcaagaat
     1561 ttattaaaat ttattagaat ttatcaacca ttaattctgt ccgtaatatg catttttgcc
     1621 atgtttcctt aaaaaatgct tatccaacag tttttgatcc attgcttctt taccaggaga
     1681 aagcatcatg gtatgataag tcatttttgc tacttcttcc ataaccacgg aattatgaac
     1741 agcttctaaa ggatcttttc cccatgaaaa aggtccgtga ctatgaacca atacccctgg
     1801 tatctccata ggatttaaat ttctaaaggt ttcaaccact actttacctg tctcttcttc
     1861 ataatttcct tctatttctt ctgctgtcat ttctcttgta cagggaatat ttccataaaa
     1921 ataatccccg tgagtagttc ccacagctgt tatactcttg ccagcctgtg cccatgaagt
     1981 agcccatggg gaatgggtat gcaccacgcc acctatatca gggaaatgct tatataatac
     2041 cagatgcgtc ggcgtatcag aagaaggcct taaactgcct tctacaatat ttccagctaa
     2101 atcaactaca actaatttat cttctgtcag ttcttcatac tttaccccgc ttggttttat
     2161 caccaccaaa cctttttccc tgtcaatacc gctaacattt ccccaggtaa aaattacaag
     2221 attgtgtcta accagagcta agtttgcctc taaaacaatt ttctttaatt ccgtcaacat
     2281 attat
//
LOCUS       DAOSUD010000155         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_20315_length_2182_cov_25.863188, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000155 DAOSUD010000000
VERSION     DAOSUD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20315_length_2182_cov_25.863188"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLG49_10940"
     CDS             <1..557
                     /locus_tag="PLG49_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ17858.1"
                     /translation="IERDGDTRKDYQPERDFIVRNIVKDIRHNKELIAEIDDELRKLI
                     PLTGYKLHTMPGIDLVTEAQIISEIGDINRFPDSDKLDRFMGLAPVQFSSAGKGKNQR
                     CRNGNRALNAIFHFLAIQMVAISASGKPRHPVFREYFEQKVKEGKNKPQALMCMARRL
                     VRIIYGMMKTKTEYRPYEKVDGKN"
     gene            1243..1446
                     /locus_tag="PLG49_10945"
     CDS             1243..1446
                     /locus_tag="PLG49_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ17859.1"
                     /translation="MFWSGLVIGIFIGAGLGILVMSMAISVGRRDYDADYRTYYETEH
                     SEELVPDLEDKDKKIDRSSDESL"
     gene            1579..2115
                     /locus_tag="PLG49_10950"
     CDS             1579..2115
                     /locus_tag="PLG49_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ17860.1"
                     /translation="MIKRSIHSERMLIRDLEEGDFSFIKQFYEDYHFYKYAIGSFKEM
                     TKDHFNALLEELKRQDDYFYCVSKLMDTDVYVGAIKGSLHFGHERILWIHLLMIDKKY
                     LRKGYGRELFETIVKYFKEILNIKWIYISVAQKNVSGHSFWIKQGFSDFKTIHPAQNE
                     SNNAFGDVMIYRKNAQEI"
BASE COUNT          773 a          290 c          456 g          663 t
ORIGIN      
        1 tgattgaaag ggatggagac acaagaaagg actatcagcc cgaaagagat tttattgtga
       61 gaaacattgt aaaggatatc aggcacaaca aggagttgat tgccgaaatt gacgatgaac
      121 taagaaagtt gatacctttg acaggctata agctacatac aatgcctggt atcgaccttg
      181 ttacagaagc gcagattata tctgaaatcg gagatattaa ccgcttcccc gattccgata
      241 agctggatcg gtttatgggc ttggcaccgg tgcaattcag ctctgccgga aagggtaaaa
      301 accaaagatg caggaatggc aatagggcac taaatgcgat atttcacttt cttgcaatcc
      361 agatggtagc aatatctgcc tcaggaaagc ctagacaccc ggtatttcga gaatacttcg
      421 agcagaaggt taaagagggc aagaacaagc cacaggcgct tatgtgcatg gcaaggcggc
      481 ttgtgaggat aatttatggt atgatgaaaa ccaagacaga atatagaccg tatgaaaaag
      541 ttgatggaaa gaattgattt tgaaaaaagt aggctgaaag ctttgaaagt attttcttct
      601 tttgattgag atattggagc ggaaataata taatggtttt agtccttgta gtgagggaac
      661 gagtaagact tggacttctc cagataaata tgtaggtaaa acagcaaacg ctatcgaagc
      721 acgataccca ggtaaagtcg ttgatgttaa taaaattgta tacagacccg atggtacgat
      781 tctgaccgac tttgacatag aattggataa tactataata caagtaaaag ccggtacagc
      841 aaagggtcta acttcacaaa taacaaaaac tgctgcggga acgaccaaag aagtaatagg
      901 gtttgccaga attaaatcct tcttcagcat ggatgtctgg cagaaatagt tatggtcagt
      961 ttggtgatgg taaaataatc aacagtagtg taccagtcca ggtgaaattc caataatgct
     1021 ttgataatct gtttgaagat gaaataatat ttttaaggaa aataattggt cataaattat
     1081 ttaaagataa aaacggtatc tgttaacaga taccgttttt aaataagaaa agttacagtt
     1141 tttatctttt tctaaaatct gtttgaatga aaaaatttat aaagacatat tctaatctta
     1201 taaaattcaa tttaatactt agccgggaaa gagggattgg atatgttttg gtctggcctt
     1261 gttataggta tttttattgg agcaggtctt gggattttag tcatgtctat ggcaatttct
     1321 gtaggacgaa gagattatga tgccgattac agaacttact atgaaacaga acattcagaa
     1381 gaactggttc ccgatttaga ggataaggac aaaaaaatag acaggagcag tgatgaaagc
     1441 ttatagagga ttctataggc ttttttattt tataaaaaat ttgcttatca ataattagaa
     1501 agctttaacc aattctttaa tttgccccag gattttttta tgttatatta aaaatatatt
     1561 attggttagg ggtaaggtat gattaagaga agtattcatt cagaaagaat gttaataagg
     1621 gatttggaag aaggagattt ttcttttatt aagcagtttt atgaggatta tcatttttat
     1681 aaatatgcca ttgggtcatt taaggagatg actaaagatc attttaatgc cctgcttgaa
     1741 gaactaaaaa gacaagatga ttatttttac tgtgtttcaa aactcatgga tacagatgtt
     1801 tatgttggtg caattaaagg ctctttgcat tttggccatg aaagaattct ttggattcat
     1861 ttgcttatga ttgacaagaa atatttaaga aaaggttatg gcagggaact gtttgaaaca
     1921 attgttaaat attttaaaga aatattaaat attaaatgga tttatatcag tgtagcacaa
     1981 aaaaatgtat ctggtcattc cttttggata aagcaaggat tttcggactt taagacaatt
     2041 catcctgccc aaaatgaatc caataatgcc tttggtgacg tgatgattta tcgtaaaaat
     2101 gctcaagaaa tttaaaataa gataacattt gggaagttgt aaaataaaat gccgtaatat
     2161 atagaaaact catggtaatc ca
//
LOCUS       DAOSUD010000156         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_20992_length_2131_cov_26.266859, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000156 DAOSUD010000000
VERSION     DAOSUD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20992_length_2131_cov_26.266859"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..826)
                     /locus_tag="PLG49_10955"
     CDS             complement(131..826)
                     /locus_tag="PLG49_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOQ17861.1"
                     /translation="MNLFDLLILIFLAFSCWVAYKRGLVLSLFKLVSRIISIFLSYKL
                     YPVVSAFLREMTPLYENIKGKISPSLLPESTAQANTLAGQSAIINNLGIPGFVKSSLI
                     ENNNPEIYKILNVDGLKDYIAGYIANICINIISLILVFIIVSIALRIIIGILDLISKL
                     PVLSSINNLFGLIFGLISGLINIWFFFIILFILQPIPAFEKVFVYLDQSLIAKSLYEY
                     NLLLKWISGIFVF"
     gene            complement(844..2031)
                     /locus_tag="PLG49_10960"
     CDS             complement(844..2031)
                     /locus_tag="PLG49_10960"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOQ17862.1"
                     /translation="MSETKQKKKKGQMIFLILLLIVVGFLAYLELSGSKLHLSFLTGN
                     QPTLYFEKKWLDINLDERAIFNIYSNDLIQCTPDGIKRISQDGQEVWNQTYSMTTPKL
                     KVNEPYIAVGEEEGKKIIVLNDKGLVYSVTTEEPIIDFSISSGGYLTYIGETKDGHTI
                     KVFDNKGNNIGLNRITYIEDAGYPLTSIISKDAKAMPISYFNPATNGLRSNIICLDLT
                     EYGILKEDLIDYGVEEEGTIIPTLYYLGNNTLVAIGDDRILWIDENKNINREFLTNRI
                     QAIPWNIESKRNLSSYLVLALAEPLPGVQGEEKGTLVFYNAKGKKEHSYQAEGDVTYL
                     YADDSMVIVGVNRNFYGLDTKGRELFKYTALKDVKEILPLKNGKKVAIISKDSVDLME
                     IKR"
BASE COUNT          696 a          373 c          308 g          754 t
ORIGIN      
        1 ctttacgata cctgctgtca taaaaaagtc acccgtcccg ggtgactttt tttatatcaa
       61 tcttttgctt gtttttaaag aaaaaaatac ttgattagca aagaatagtt caaagttata
      121 ttttattttt ttaaaaaaca aatattcctg aaatccattt taaaagaaga ttatattcat
      181 acaaagactt tgcaatcagg ctttgatcca gataaacaaa gactttttca aaggccggga
      241 ttggctgtaa aatgaataaa atgataaaga aaaaccagat atttattaga cctgaaatca
      301 agccaaagat tagaccaaat aggttattga ttgaactaag cacaggtaat tttgatataa
      361 ggtctaaaat accaatgata attcttaaag caatggatac gatgataaat actaaaatta
      421 atgaaataat attgatacat atattggcaa tgtaacctgc aatataatcc tttaatccgt
      481 ctacgtttaa aattttatag atttcgggat tattgttttc gattaaggag ctttttacaa
      541 aaccaggtat tcctaaatta ttgataatcg cactctgccc agctaaggta ttggcttgtg
      601 ctgtactttc cggtaaaagc gatggagaaa ttttaccttt tatattttca taaaggggag
      661 tcatttctct taaaaaagca ctgacaacag gatacaattt ataggataaa aatattgaaa
      721 taatacggga taccaattta aataaagaaa gaactaaccc tcttttataa gctacccagc
      781 aactgaaagc caaaaaaatt aagattaaaa gatcaaatag attcatgact tttcccccaa
      841 atcttatctt tttatttcca ttaaatctac agaatcctta gatattatgg caactttttt
      901 tccatttttt aatgggagta tttctttgac atcttttagg gccgtatatt taaataattc
      961 tctgcctttg gtatctaatc catagaaatt tctgtttact ccaacaataa ccatgctgtc
     1021 atcggcatat aaataggtca catccccttc cgcctgatag ctgtgttctt ttttgccttt
     1081 tgcattgtaa aatacaaggg ttcccttttc ttctccctgt acacctggaa gaggctctgc
     1141 caatgccaat actaaataag aggataaatt tcttttactt tctatattcc aggggattgc
     1201 ttgtattcgg tttgttaaaa attccctatt gatattttta ttttcatcaa tccaaagaat
     1261 cctgtcatcg cctatggcca ctaatgtatt atttcccaga taataaaggg ttggtataat
     1321 ggttccttct tcttcaaccc cataatcgat gaggtcttct tttaaaatac cgtattcggt
     1381 taaatccaga caaataatat ttgatcttaa gccattggtt gccgggttaa aatagctgat
     1441 tggcattgcc ttggcatctt tagaaataat agatgtcaga ggataaccgg catcttctat
     1501 ataagtaatt ctatttaaac cgatattatt tcccttatta tcaaaaacct tgatggtatg
     1561 accgtctttt gtttctccta tataggttaa atagcctcct gaactaattg aaaaatctat
     1621 aataggttct tctgtcgtca cactataaac caaaccttta tcattcagaa caataatttt
     1681 cttgccttcc tcttctccta ctgcaatgta aggttcattg acttttaatt tgggtgttgt
     1741 catggaatat gtctgattcc agacttcctg tccatcttga gatatacgct tgattccatc
     1801 aggggtgcat tgaattaaat cattagaata aatattaaat attgcccgct catctaaatt
     1861 gatatccagc catttttttt caaaataaag ggtgggctga tttccagtta gaaaagaaag
     1921 atgaagctta cttcctgaca gctccagata agctaagaag cctaccacga ttagtaaaag
     1981 aataagaaag atcatttgtc ctttcttttt cttttgtttt gtctcactca acttttttcc
     2041 ccctaatcat ccaagaagta aatttataaa aagttattta ttagtatcgt actataagtt
     2101 atttatgatt tcaacatttc cgttttatat c
//
LOCUS       DAOSUD010000157         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_21554_length_2093_cov_29.570167, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000157 DAOSUD010000000
VERSION     DAOSUD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_2093_cov_29.570167"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1061
                     /locus_tag="PLG49_10965"
     CDS             81..1061
                     /locus_tag="PLG49_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ17863.1"
                     /translation="MEINEKNIQEFSTRFLEIEKAISEVIIGQKDIIKKVLMAIIAGG
                     NVLLEGLPGLGKTQLVKTIGKVMDLQFSRIQFTPDLMPADVVGTNIIVKKPDGTNEFQ
                     FQPGPVFSNIVLADEINRATPKTQSAMLEAMQEKTVTVGNNTYYLPSPFFVLATQNPL
                     EMEGTYPLPEAQMDRFMFKLDVKFPTQEELAEIVRLTTGKEAPEARQVCDKNTLIEMG
                     VIAKEVPVATAVYEYAMSVVLKLHPESENAPEATKKYIHYGPSPRGAQAIITAAKINA
                     LIDGRYNVSFDDIKEVALPTLRHRIFLNFEALSDKVTADDLILEVLKDGK"
     gene            1064..1936
                     /locus_tag="PLG49_10970"
     CDS             1064..1936
                     /locus_tag="PLG49_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ17864.1"
                     /translation="MPKLVEVFNKDYIGKLESFSIYMDTLLMNGYSGARKSKAKGMSL
                     EFSDFRPYVLGDDIRRIDWNSYGRFDKLFIKLFMEEKQGSINIFIDSSASMQAGEPEK
                     LFYAKQLAASIAYIALRNMDLVNLFAFSDEITNEKKNIQTKNLFPDLLDFLDNLPVNK
                     ESRLVNALNKIKNIPMTKGISFIISDFFSEDGYEEAVKLLQYKKQQIVLVQILSPEEI
                     DPTIRGNLRLVDSESGKAKDIELTQDRINNYKKALEKFKNQIQTFCTKRGQQYIFLST
                     DIPILLGLENCLRR"
BASE COUNT          723 a          305 c          404 g          661 t
ORIGIN      
        1 ttctttttta tttttagttg acttagcttt tttactcggc tacaatgata ttaaaaaaat
       61 tgaggcaatg gtggttaaat atggagatta atgagaaaaa cattcaagaa ttttcaacaa
      121 gatttttgga aatagaaaag gctatatcag aagtcattat tggacaaaaa gatattatta
      181 aaaaggtttt aatggctatt attgcgggag gaaatgtgct tttagaaggt cttccgggac
      241 tgggtaagac acagttggta aagaccatag gaaaggtaat ggatttacag ttttcaagaa
      301 ttcagtttac accagattta atgcctgccg atgttgtagg tacaaatatt attgtaaaaa
      361 agcctgatgg aacaaatgaa tttcaatttc agccagggcc tgttttttct aacattgttt
      421 tagccgatga aataaacagg gcaactccaa aaacccaaag tgccatgtta gaagccatgc
      481 aggaaaaaac cgtaaccgtt ggaaataata cttattattt accaagtcca ttttttgttt
      541 tggcaacaca aaatcctttg gagatggaag gcacatatcc tttgcctgag gctcaaatgg
      601 acagatttat gtttaaattg gatgtaaaat ttccaacgca ggaagagctt gctgaaattg
      661 tacgcttaac aactggaaaa gaggcgcctg aagccagaca agtttgtgat aaaaatactt
      721 taattgaaat gggagtaatt gcaaaggaag tgcctgttgc gaccgcagta tatgaatatg
      781 ccatgagtgt tgtattaaaa cttcatcctg aatctgaaaa tgccccggaa gcaacgaaaa
      841 agtatattca ttacgggcca agccccagag gagctcaggc gattattaca gctgcaaaaa
      901 ttaatgcttt aattgatggt cgttataatg tatcttttga tgatataaaa gaagtagccc
      961 tgccaacttt aaggcatcgt attttcctga actttgaagc tctttcagac aaagtaacag
     1021 cagatgattt gattttggaa gtattaaagg acggtaaata attatgccaa agttggttga
     1081 ggttttcaac aaagattata ttggtaaatt agaatctttt tcaatttata tggatacgct
     1141 tcttatgaat ggttatagcg gtgccagaaa gtctaaagca aagggaatgt cccttgaatt
     1201 ttcagatttc cgtccctatg tcttaggaga cgacatcaga agaatagact ggaatagtta
     1261 cggtcgattt gataagttat ttataaaact gtttatggaa gaaaagcagg gttccattaa
     1321 tatttttata gatagcagtg cttccatgca ggccggagaa cccgaaaaac tgttttatgc
     1381 caagcaatta gcagcttcta tagcctatat tgccttaaga aatatggacc ttgttaattt
     1441 atttgccttt agtgatgaga ttaccaatga aaagaaaaat attcaaacga aaaacttgtt
     1501 tcctgatttg ctggattttt tggataactt gcctgtaaat aaggaatcca gactggtgaa
     1561 tgcgttaaat aaaataaaaa atattcccat gacaaaggga atttctttta ttatttctga
     1621 tttcttttca gaagatggtt atgaagaggc agtgaagttg cttcaataca aaaagcagca
     1681 gattgtgctg gttcagattt tatctcccga ggaaatcgat ccgacaatcc gtgggaattt
     1741 aagattagtc gattctgaaa gtgggaaagc aaaagatatc gaattaacac aagacagaat
     1801 caataattac aaaaaggccc tcgaaaaatt caaaaatcaa atacaaactt tttgtacaaa
     1861 aagagggcag caatatatat tcctttctac agatattcct attttacttg gtctggaaaa
     1921 ttgtttaaga agataaaaag atgcttttag catctttttt acattttaca gataattatg
     1981 cttaaatcat caatagaaca tctgctttca ggattatcat tgtaaaactt atttaggata
     2041 atttcaataa tttcttctgg tggatattct ttatattgaa gaataagttt ttt
//
LOCUS       DAOSUD010000158         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_22350_length_2039_cov_22.126512, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000158 DAOSUD010000000
VERSION     DAOSUD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22350_length_2039_cov_22.126512"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..895
                     /gene="rph"
                     /locus_tag="PLG49_10975"
     CDS             155..895
                     /gene="rph"
                     /locus_tag="PLG49_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOQ17865.1"
                     /translation="MERIDKREYAELRPVRITRHYIKYPEGSVLIEMGDTKIICNATV
                     EEKVPPFRKGTGEGWVTAEYSMLPRATEVRNSRDISRLKMNSRATEIQRLIGRSLRSV
                     MDFSLLGEKSIILDCDVIQADGGTRTASITGAFVALADACHYLLEQGSIEKMPIKSFV
                     SAISVGIVEGIPMLDLCYDEDSRAEVDMNIVMTDKGEYVEIQGTGEKAPFSSETFNQL
                     IELGSIGIKQLIHNQKEVLGDLSKLIGG"
     gene            899..1507
                     /locus_tag="PLG49_10980"
     CDS             899..1507
                     /locus_tag="PLG49_10980"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOQ17866.1"
                     /translation="MRRIIFATKNQGKIAEIQEIMKELNLEILSLESAGIDTDIIENG
                     SSFEENAIIKVKEIMKLTNDIVMADDSGLEIDYLNGAPGVYSSRFEGENTPYDIKNNK
                     ILSMLEGVPDEKRTARFVCVIAAGFPNGEIITTKGTIEGRIGYEIKGENGFGYDPIFY
                     VPEYNMTTAQMSPQLKNQISHRAKALNQMKAQLRIRLGEILE"
     gene            1504..2022
                     /locus_tag="PLG49_10985"
     CDS             1504..2022
                     /locus_tag="PLG49_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ17867.1"
                     /translation="MRLLILSDSHNHLYYARKVIEKLKDRIDLVIHLGDHDKDALVLN
                     SEFPEINLDYVKGNCDFDSSSPSEKMIHLSGKKILMLHGHNHHVKWGYDRIAYYGQEK
                     EADILLFGHTHVPFMDYYGKMLLLNPGSISLPRATSIPTFGIINFEKEGKVEVAIMTV
                     NKNASIDRLRSF"
BASE COUNT          717 a          273 c          444 g          605 t
ORIGIN      
        1 ataaaaattt ttattcgaat tatatcttgt gataattttg ttttatggta aaataagtac
       61 ggttatacag cataactttt taactgtgat aaaatgaaga atagcaatat atttttcggt
      121 gaagaatagc caatagagaa aggatgaatg tattttggaa agaattgata aaagagaata
      181 tgctgagcta aggcctgtaa gaataactcg tcactatata aaatatccgg aaggttctgt
      241 actgattgag atgggagata cgaaaattat ttgtaatgca acggtagaag agaaagttcc
      301 tccttttaga aaaggaacag gagaaggttg ggtaacagca gaatattcta tgttaccgag
      361 ggctacagaa gtaagaaatt ccagagacat cagtcgtttg aagatgaata gccgtgctac
      421 tgaaatccaa agactgattg gccgttctct tcgctctgtt atggattttt ctttgctggg
      481 agaaaagtcc attattctcg attgtgatgt gatacaggct gacggaggta cccggacggc
      541 gtcaattact ggtgcatttg tggctttggc cgatgcctgt cactatcttt tagaacaagg
      601 cagcattgaa aaaatgccca ttaaaagttt tgtttctgct atcagcgtcg gtattgtgga
      661 aggaatacct atgctggatt tatgctatga tgaagacagc agggcagaag ttgatatgaa
      721 tattgttatg accgataagg gggaatatgt agaaattcag gggactgggg aaaaggctcc
      781 tttttcttca gagactttta atcaactgat tgaattgggc agtataggta ttaaacaatt
      841 aattcataat caaaaagaag ttttaggaga tttatcaaag cttattgggg gttaaacgat
      901 gagaagaatt atttttgcaa caaaaaatca aggaaaaatt gcagaaatcc aagaaattat
      961 gaaagaatta aacttagaga tcctttcttt agaatcagca gggattgata cagacattat
     1021 agaaaatggt tctagctttg aagaaaatgc aataattaaa gttaaagaaa ttatgaaact
     1081 gacaaatgac attgtcatgg cagatgactc gggcctggaa atagattatt tgaatggggc
     1141 accgggtgta tattcttcca gatttgaggg agaaaatacc ccgtatgata ttaaaaataa
     1201 taagatttta agcatgttag aaggggtacc agatgaaaaa agaactgcaa gatttgtttg
     1261 tgtgattgct gcaggttttc caaatgggga aatcattacc acaaaaggta cgattgaagg
     1321 caggataggc tacgaaataa aaggtgaaaa tggctttggt tatgacccta tattttatgt
     1381 tccagagtat aatatgacca ctgctcaaat gtccccacaa ttaaaaaatc aaatcagtca
     1441 cagggcaaag gcattaaatc agatgaaagc acaactcaga atcagactgg gggaaatact
     1501 tgaatgaggt tattgatttt aagtgatagc cataatcatt tatactatgc gagaaaggtt
     1561 atagaaaaat taaaagacag aatagatctg gtgattcatc tgggagatca tgataaagat
     1621 gctttagtat taaattctga atttcctgag attaatttgg attacgtaaa aggaaactgc
     1681 gactttgaca gttcttctcc ctcagaaaaa atgattcatc tgtcagggaa aaagatactt
     1741 atgcttcatg gtcataatca tcatgtgaaa tggggctatg acagaatagc ttattatggt
     1801 caggaaaaag aagcggatat tcttttattt ggtcatactc atgtaccgtt catggattat
     1861 tatggaaaga tgcttttatt aaatcctgga agcattagtt tacccagggc tacgagtatt
     1921 ccaacttttg gaataatcaa ttttgaaaaa gaaggtaaag tagaggttgc tattatgaca
     1981 gtcaacaaaa acgcttccat cgacagattg aggagctttt gattttttta aaaaactat
//
LOCUS       DAOSUD010000159         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_24283_length_1924_cov_25.373462, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000159 DAOSUD010000000
VERSION     DAOSUD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24283_length_1924_cov_25.373462"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..>1924
                     /locus_tag="PLG49_10990"
     CDS             264..>1924
                     /locus_tag="PLG49_10990"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOQ17868.1"
                     /translation="MDEFIKQLDPNLDYISHEIKDGKCYITVTSNRKEVTCPFCGWPS
                     SRIHSTYNRTFQDLPIQGNKVFIIIRNRKFFCDNSDCNHTTFAERFDFISYKAKKTRR
                     LEDEIVRLSINCSSITASKILKENVVDIGKSTVCNLLKKKETPVIDKKTITAVCIDDF
                     AIKKRRSYGTIMVDIFTHQILDMIDSRDYETVCEWLKSYPNLRIISRDGSVTYNNAIT
                     GAHPEALQISDRFHLLKNLTSYVTEYLKKRLKPQVSIQSVSQEIKEVETIKQANENRK
                     LTLKEKYEKIKQLSLEGRCKTEICRSLNMDIRAYDKLIAMTPEEREKLFQTKNMIIHE
                     EKVKQKMERINEVRELKRIGLSNIEISRRTGLDRKTVRRYLDENFNPVHASYGKKRNG
                     KLTPYIKTIDEYLERGMMGSYIVEKIHEMGYDGSSSTVRHYMTDWKKRRKKYYDRSRE
                     DGTKTEIIKRENIFKLLYHPIEKVKIISEKQFEMICKEYPFFEKIYKITWEFKDMLTN
                     KNIEAFDKWIERAKNLNIPEINSFINGVERDMEAVRNAIKYEYSNG"
BASE COUNT          793 a          255 c          364 g          512 t
ORIGIN      
        1 tgagcgacca tattgccaag cgtgacggta ccaagatttt cattgaaatc agtacagcag
       61 atgccacaga agaggatata aagaatctct acgaccttct ttccaagctg tttgacgaaa
      121 caaaggaaaa ttactcacca atggttaatt acatggttgt cagtggtaaa atttaattgg
      181 ttctttccag ggttaattga attaaatgac aatatatgat aaaataaatg aaaattaaac
      241 aaaagaaaag aggattatat tccatggatg aatttattaa acaattagac ccaaacttag
      301 actatattag tcatgaaata aaagatggca aatgctatat aacagtaact tccaatcgaa
      361 aagaagtaac atgcccattt tgtggctggc catcatccag aatacattcc acatataaca
      421 gaacctttca ggaccttcca atacaaggta ataaggtatt tattattata cgtaacagaa
      481 aatttttttg tgataattct gattgtaatc atactacctt tgcagaaagg tttgatttta
      541 tctcctataa agcgaagaaa acccgccgtc ttgaggatga aattgtgcga ctatcaataa
      601 attgcagttc cattacagca tcaaaaatac taaaggaaaa tgttgtggat attggtaaaa
      661 gtacagtttg taatctttta aaaaaaaaag aaacaccggt aattgataaa aagactataa
      721 cagctgtttg tattgatgat tttgctatta aaaagcgtag aagctatgga acaattatgg
      781 tagacatttt tacgcatcaa atacttgata tgattgactc aagggattat gagactgttt
      841 gtgagtggtt aaaatcatat ccaaatcttc gtataatatc aagagatggg tctgtcacct
      901 ataataatgc aattacaggt gcacacccgg aagctttaca aataagtgac cgttttcatt
      961 tgctaaagaa tttgacttcc tatgtaacag agtatctaaa aaagagatta aaaccgcaag
     1021 tttcaataca atctgtcagt caggaaatta aagaggtaga aacaataaaa caggcgaatg
     1081 agaacagaaa actcacattg aaagaaaaat atgaaaagat aaaacaactt tcattagaag
     1141 gaagatgtaa gacagaaatt tgccgaagct taaatatgga tatacgagct tatgataaat
     1201 taatagcaat gacccctgaa gaaagggaaa agttgttcca gacaaaaaat atgatcatac
     1261 atgaagaaaa agtaaagcaa aaaatggaac gtataaatga ggtgcgggag ttaaagagaa
     1321 taggtttgag taatatagag atatccagac gtaccggact tgatagaaaa acagttagaa
     1381 gatatcttga tgaaaacttt aatccggttc atgcttccta tggcaaaaag agaaatggga
     1441 aactgacacc atatataaaa acgattgacg aataccttga gagaggaatg atgggttcat
     1501 atattgtgga aaagatacat gaaatgggat atgatggttc gtcatcaact gtgcggcatt
     1561 atatgacaga ctggaagaaa cggagaaaaa aatattacga tagaagtaga gaagatggga
     1621 caaaaacaga aataataaaa agagaaaata tatttaagct attgtaccac ccaatagaaa
     1681 aagtaaaaat aatcagtgag aaacaatttg aaatgatatg caaagaatat ccgttttttg
     1741 aaaaaatata taaaataaca tgggaattta aggatatgct aactaataaa aatattgaag
     1801 ccttcgataa atggatagag cgggctaaaa atctaaacat accggaaata aacagtttta
     1861 taaatggagt tgaacgagat atggaggctg taaggaatgc gataaaatat gaatatagta
     1921 atgg
//
LOCUS       DAOSUD010000160         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Defluviitaleaceae bacterium isolate AMR_MDS_2198
            NODE_27735_length_1750_cov_20.093805, whole genome shotgun
            sequence.
ACCESSION   DAOSUD010000160 DAOSUD010000000
VERSION     DAOSUD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864265
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Defluviitaleaceae bacterium (wastewater metagenome)
  ORGANISM  Defluviitaleaceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Defluviitaleaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:55:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,146
            CDSs (with protein)               :: 2,146
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 12 of 23
            Pseudo Genes (incomplete)         :: 12 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Defluviitaleaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27735_length_1750_cov_20.093805"
                     /isolate="AMR_MDS_2198"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660712"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1179
                     /locus_tag="PLG49_10995"
     CDS             310..1179
                     /locus_tag="PLG49_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOQ17869.1"
                     /translation="MKRKFKDLGIAILYTLTALGMQLLTTLAVTLLGGFLIGYNYTIN
                     NPEESLSEELIVEFVNNFVTEVTPYILIIASLSAFVIYLLVIKLYNKSFIKYTNFTKP
                     KMSLLALSAIIGIALNLAVGLIISLTGAETYFPEHEEVINSIVGELAIQNIIVTAITA
                     PLFEEILFRGLIFRQVKKSFPVAAAVIIQSILFALLHGNTLQMLYTFILGVSAALVCQ
                     WGDSLWNAIVLHIFYNGTSLILTIINMDFTLLTALITMTISSVIYIAGMIICYKKRCD
                     IQTAFVNNYIASI"
     gene            1373..1726
                     /locus_tag="PLG49_11000"
     CDS             1373..1726
                     /locus_tag="PLG49_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOQ17870.1"
                     /translation="MDYHEPIELLDKRARDIARMLHSLKEEVEAVNWYHQRVSASDNP
                     EVKEILAHNRDEEIEHAVMAIEWLRRNAPGWDEELRKYLFTTGPIVGLEEEANSFDKP
                     LSLKNSQLNIGSMKN"
BASE COUNT          631 a          278 c          273 g          568 t
ORIGIN      
        1 ataaaaataa aaaaacatcg ggtaaactgt aaaataaacg atttaaaaga attaataaca
       61 aattatattt tatcattatt tttaaaaaaa gaaaataaat tttaaaaaaa gctcaaataa
      121 agtatttttt agaattatta ataagtatat tataaattcc tataaagaac gctcattctt
      181 gatatgtgta aaaaatattg aaataattat aaatcgaaga attgttcttt acaaattaaa
      241 tacttattca ttaaaataca tagagatggt tttttatcgt ctatttacaa aaattatgga
      301 ggtcttcaaa tgaaaagaaa atttaaagat ttaggtattg ccattcttta tactttaact
      361 gctttgggga tgcaattatt aacaacatta gcggttacat tattgggagg atttcttatt
      421 ggatataatt ataccataaa caatccggaa gaaagcttgt ctgaggaatt gattgtcgaa
      481 ttcgtcaata actttgtcac tgaggtgact ccctatattt taatcatcgc ttctttatcc
      541 gcttttgtga tttatttgct ggttataaaa ctatataata aaagttttat aaaatataca
      601 aattttacta aacctaaaat gtctttgtta gccctgtctg ccataattgg aattgcatta
      661 aaccttgcag taggcttaat tattagcctg acaggtgcag aaacttattt ccctgaacat
      721 gaagaagtta tcaattctat cgtaggagaa ttagcaatac aaaacatcat tgttacagcg
      781 attaccgcac ctttatttga agaaatactt ttccggggac tcattttcag gcaagtaaaa
      841 aaatctttcc ctgttgcagc cgcagtaatt attcaatcta tactttttgc cttattgcat
      901 ggaaataccc ttcaaatgct ttatactttc attttaggag tatctgcagc acttgtctgc
      961 cagtggggag attctctttg gaatgccatt gtcctgcata tattttataa tggaacatcc
     1021 ctgattttaa ctattatcaa catggatttt accctgttaa ccgccttaat cacaatgacc
     1081 atttcctctg tcatttatat cgccggcatg attatttgtt ataagaagcg atgtgatatt
     1141 cagacagcat ttgtaaacaa ttatatagca agtatatagc acgccagccc tgattatttg
     1201 acaaactaaa atatataact tcaatacata aaagaaccgc ttaaggttct ttttttattg
     1261 actgctgatt cctaatttat aaataaatac agtccatcct ttgaataata aaataaatac
     1321 ggcataaaat agctatgata aatatacaat ccaactaaag aggaggcata tgatggacta
     1381 ccatgaacct atagaattac tggacaaaag agcaagggat attgccagaa tgcttcatag
     1441 tttaaaagaa gaagttgaag cagtaaactg gtatcaccag agagtttctg cttctgacaa
     1501 tcctgaagtt aaagaaatac ttgcacataa ccgggacgaa gaaatcgaac atgccgtcat
     1561 ggcaatagaa tggctaagaa gaaatgcgcc aggctgggat gaagaactcc gtaaatactt
     1621 atttaccact ggtcccattg ttggactgga agaagaagct aattcttttg acaaacccct
     1681 aagcttaaaa aactcacagc tcaatatcgg cagtatgaaa aactaaagga ggcattgtaa
     1741 tggacatttt
//
